SMED30019412

Overview
NameSMED30019412
Smed IDSMED30019412
Length (bp)869
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019412 (SMED30019412) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019412 (SMED30019412) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019412 (SMED30019412) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019412 (SMED30019412) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019412

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30019412h1SMcG0022309 SmedASXL_069112SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30019412h1SMcG0022309 dd_Smed_v4_6474_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30019412h1SMcG0022309 dd_Smed_v4_6438_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019412h1SMcG0022309 dd_Smed_v4_6438_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019412h1SMcG0022309 dd_Smed_v4_6438_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019412h1SMcG0022309 dd_Smed_v4_6438_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30019412h1SMcG0022309 dd_Smed_v6_6474_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parenchymal cellSMED30019412h1SMcG0022309 dd_Smed_v6_6438_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019412h1SMcG0022309 dd_Smed_v4_6438_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30019412h1SMcG0022309 dd_Smed_v4_6438_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019412 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.010632:6332..9098 -4021
Homology
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BLAST of SMED30019412 vs. Planmine SMEST
Match: SMESG000023122.1 (SMESG000023122.1)

HSP 1 Score: 440.269 bits (1131), Expect = 2.229e-156
Identity = 233/237 (98.31%), Postives = 233/237 (98.31%), Query Frame = 1 
Query:   88 QSLKPRKLSCPLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMKKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFREFTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTXXXXXXXXXXXXXXXXXXYIRYLAVIIHPARFIKNLGDG 798
            QSLKPRKLSC LNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVM KKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRE TKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNL DG
Sbjct:  115 QSLKPRKLSCQLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMNKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRECTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNLSDG 351
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BLAST of SMED30019412 vs. Planmine SMEST
Match: SMESG000023122.1 (SMESG000023122.1)

HSP 1 Score: 439.884 bits (1130), Expect = 8.690e-156
Identity = 233/237 (98.31%), Postives = 233/237 (98.31%), Query Frame = 1 
Query:   88 QSLKPRKLSCPLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMKKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFREFTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTXXXXXXXXXXXXXXXXXXYIRYLAVIIHPARFIKNLGDG 798
            QSLKPRKLSC LNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVM KKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRE TKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNL DG
Sbjct:  142 QSLKPRKLSCQLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMNKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRECTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNLSDG 378
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BLAST of SMED30019412 vs. Planmine SMEST
Match: SMESG000023122.1 (SMESG000023122.1)

HSP 1 Score: 434.876 bits (1117), Expect = 4.151e-154
Identity = 233/240 (97.08%), Postives = 233/240 (97.08%), Query Frame = 1 
Query:   88 QSLK---PRKLSCPLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMKKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFREFTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTXXXXXXXXXXXXXXXXXXYIRYLAVIIHPARFIKNLGDG 798
            QSLK   PRKLSC LNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVM KKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRE TKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNL DG
Sbjct:  115 QSLKFIQPRKLSCQLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMNKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRECTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNLSDG 354
[Close]
BLAST of SMED30019412 vs. Planmine SMEST
Match: SMESG000023123.1 (SMESG000023123.1)

HSP 1 Score: 129.798 bits (325), Expect = 1.325e-34
Identity = 75/175 (42.86%), Postives = 108/175 (61.71%), Query Frame = 1 
Query:  148 IFNKDYEIPEYQPEAQTMNGGYITLETFP-FRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMKKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFREFTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEIN 669
            IF  DYE+PEY  + + + GGY +LE F  FR+ NT  +S++F+AMKIREEV N GGV + + +  + + +VK+++ KLW+  +   N  N  LHVGDRI++VN R   D+  F++   N    I I+QRLPKA+VF +D    + + IKL K   +    TK +   W ITEIN
Sbjct:  205 IFAYDYEVPEYSSQVEPLFGGYESLEKFEKFRSDNT--NSSVFLAMKIREEVLNRGGVKVELDEEFLHSFKVKLIENKLWVCHVNINN--NNKLHVGDRIVTVNGRLPKDVFDFQQICHNSRASI-IIQRLPKAKVFAYDMINSNRQGIKLFKEKLYFKINTKFRREKWKITEIN 374
[Close]
BLAST of SMED30019412 vs. Planmine SMEST
Match: SMESG000023120.1 (SMESG000023120.1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.404e-18
Identity = 58/173 (33.53%), Postives = 87/173 (50.29%), Query Frame = 1 
Query:  160 DYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMKKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFREF---TKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEIN 669
            +Y I  ++   Q ++G    L  FP     +  DS+IF   K+REE  + GGV I V    I  +++ V + K+W++     N++  +LHVGD+IIS+N+  V DL  F +F   TK  ED  L + RLPK RVF    +  +        N     + T     +W ITEI+
Sbjct:  108 NYNIDSFRLNVQPLDGNRNNLFHFPIIENTSNLDSDIFYIAKLREESKHPGGVVINVRIADIVGLRIVVSQNKIWVARCPKINKIKGNLHVGDQIISLNDVLVTDLNQFIDFVRSTKKSEDIKLRLLRLPKGRVFALTPHNYNSVIFINPINQLVSFRSTGSS--NWMITEID 278
The following BLAST results are available for this feature:
BLAST of SMED30019412 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019412 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023122.12.229e-15698.31SMESG000023122.1[more]
SMESG000023122.18.690e-15698.31SMESG000023122.1[more]
SMESG000023122.14.151e-15497.08SMESG000023122.1[more]
SMESG000023123.11.325e-3442.86SMESG000023123.1[more]
SMESG000023120.13.404e-1833.53SMESG000023120.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019412 ID=SMED30019412|Name=SMED30019412|organism=Schmidtea mediterranea sexual|type=transcript|length=869bp
CTGTCTTAACGAAATCTATATAAAAATTATATGGATAAATCTGATTCCAA
CTCATTAAATGAGAACAACAGTCCAAATTCCGCAAGCCAATCACTTAAAC
CTCGAAAACTGTCATGCCCACTAAATTTCCTAGATGGCGAAAATGCAATA
TTTAACAAAGACTACGAGATTCCGGAATATCAACCGGAAGCTCAAACGAT
GAATGGCGGCTATATAACATTAGAAACTTTCCCTTTTCGAAATAAAAACA
CATTTAATGACTCAAATATTTTTGTGGCCATGAAAATTAGAGAGGAAGTA
TGCAATTTTGGAGGAGTTACAATTACTGTGGGGAAACATTTGATCGAAAA
CATCCAAGTCAAAGTTATGAAAAAAAAATTATGGATATCGGATATCCAAA
ACTGGAACGAGATGAATGCCAGTTTGCACGTTGGAGACAGAATTATCTCC
GTCAACGAAAGACCGGTAAATGACTTGCAAACCTTTCGGGAGTTTACCAA
AAATCGTGAGGACTACATTTTAATAGTACAACGACTTCCAAAAGCTAGAG
TATTTGTATTCGATAAGAACAAATTAGACGTGAAGACAATAAAATTGGAG
AAAAATAATTTCTTTTTAACAAAACCTACAAAACCGAAAGACTTGGATTG
GGCAATCACAGAAATTAATCGCACAAAAACTGTCAATATAAATGATCAAA
ACATAATTTCCAACATACAATCAGTCATTAGCTCATACATTCGGTACCTT
GCAGTCATTATCCACCCCGCCAGGTTTATCAAGAACCTCGGCGATGGCTA
ATATTTGTCTCCATTGAAATTCAGATCTTTGTCATTTTTGTCATTGTAAA
TATATGTCTTAAAAGAAAA
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protein sequence of SMED30019412-orf-1

>SMED30019412-orf-1 ID=SMED30019412-orf-1|Name=SMED30019412-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=257bp
MDKSDSNSLNENNSPNSASQSLKPRKLSCPLNFLDGENAIFNKDYEIPEY
QPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITV
GKHLIENIQVKVMKKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQ
TFREFTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPT
KPKDLDWAITEINRTKTVNINDQNIISNIQSVISSYIRYLAVIIHPARFI
KNLGDG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000106ciliated epithelial cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036034PDZ_sf
IPR001478PDZ
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
IPR036034PDZ superfamilySUPERFAMILYSSF50156PDZ domain-likecoord: 94..165