SMED30019370
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019370 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30019370 aligns in the following genomic locations:
Homology
BLAST of SMED30019370 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 172.17 bits (435), Expect = 5.247e-51 Identity = 96/112 (85.71%), Postives = 97/112 (86.61%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGEV 318 VDNSSV PE KSISNEFIE NQSNI DSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSI EYREMPSKL P NSSSI DIQHLSNRSPVDN+SVIPETGEV Sbjct: 368 VDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIINEYREMPSKLSPINSSSI-DIQHLSNRSPVDNSSVIPETGEV 478 HSP 2 Score: 133.265 bits (334), Expect = 2.541e-37 Identity = 75/102 (73.53%), Postives = 80/102 (78.43%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPE 306 V NS + PEKSISNEFIEYRNQSNIIDSVLINKSQTSIRY ESDKTFVLSNAEDGQNI+DKSI E +M SKL KN LSN+S VDN+SV PE Sbjct: 616 VKNSLLLPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKN---------LSNKSQVDNSSVFPE 708 HSP 3 Score: 114.39 bits (285), Expect = 1.037e-30 Identity = 78/134 (58.21%), Postives = 87/134 (64.93%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQN--IADK-----SIFTEYR----------------EMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGE 315 VDNSSV PE KSISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N+ +N + K S F E + E+P KLPP NSSSI DIQHLSNRSPVDN+SVIPETGE Sbjct: 247 VDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSNSERSILSEIPPKLPPINSSSI-DIQHLSNRSPVDNSSVIPETGE 379 HSP 4 Score: 108.997 bits (271), Expect = 7.966e-29 Identity = 73/108 (67.59%), Postives = 84/108 (77.78%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLS------NRSPVDNNSVIPE 306 VDNSSVFPEKSISNEFIE NQS+IIDSVLIN SQTSIRYLESDKTFVLSNAED +NIAD+SI E E+PS L +SSS+ +IQ LS N+S +D + +I E Sbjct: 700 VDNSSVFPEKSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAED-KNIADQSIINEISEIPSNLSSIDSSSV-EIQALSEFIENRNQSNIDGSVIINE 805 HSP 5 Score: 93.5893 bits (231), Expect = 2.022e-23 Identity = 55/86 (63.95%), Postives = 65/86 (75.58%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSS 240 VDNSSV PE KSISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N+++GQN+ADKSI E E+ KL +NS+ Sbjct: 466 VDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNENPEITYKLSRENST 551 HSP 6 Score: 68.1662 bits (165), Expect = 1.736e-14 Identity = 39/48 (81.25%), Postives = 40/48 (83.33%), Query Frame = 1 Query: 175 ADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGEV 318 ADKSI E MPSKL P NSSSID IQHLSNRSPVDN+SVIPETGEV Sbjct: 215 ADKSIINE--NMPSKLHPINSSSID-IQHLSNRSPVDNSSVIPETGEV 259 HSP 7 Score: 62.003 bits (149), Expect = 2.314e-12 Identity = 41/73 (56.16%), Postives = 46/73 (63.01%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDG---QNIADKSIFTEYREM 210 VDNSSV PEK ISN FIE +NQSNI S+LINK + I L S+ N E G NIA+KSI EY EM Sbjct: 859 VDNSSVIPEKPISNRFIENQNQSNIDRSILINKLRLPIECLGSN------NDESGLENHNIANKSIINEYPEM 925 HSP 8 Score: 44.2838 bits (103), Expect = 3.639e-6 Identity = 41/91 (45.05%), Postives = 52/91 (57.14%), Query Frame = 1 Query: 43 EFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDG---QNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPE 306 EFIE RNQSNI SV+IN+ Q + S+ E G NIA+KSI E + S L +SS I +I L+ +S VDN+SVIPE Sbjct: 787 EFIENRNQSNIDGSVIINEFQLPMECSGSNTN------EPGLANHNIANKSIINENQ---SNLSSIDSSGI-EILALTEQSQVDNSSVIPE 867
BLAST of SMED30019370 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 172.17 bits (435), Expect = 5.670e-51 Identity = 96/112 (85.71%), Postives = 97/112 (86.61%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGEV 318 VDNSSV PE KSISNEFIE NQSNI DSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSI EYREMPSKL P NSSSI DIQHLSNRSPVDN+SVIPETGEV Sbjct: 363 VDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIINEYREMPSKLSPINSSSI-DIQHLSNRSPVDNSSVIPETGEV 473 HSP 2 Score: 133.265 bits (334), Expect = 2.694e-37 Identity = 75/102 (73.53%), Postives = 80/102 (78.43%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPE 306 V NS + PEKSISNEFIEYRNQSNIIDSVLINKSQTSIRY ESDKTFVLSNAEDGQNI+DKSI E +M SKL KN LSN+S VDN+SV PE Sbjct: 611 VKNSLLLPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKN---------LSNKSQVDNSSVFPE 703 HSP 3 Score: 114.005 bits (284), Expect = 1.132e-30 Identity = 78/134 (58.21%), Postives = 87/134 (64.93%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQN--IADK-----SIFTEYR----------------EMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGE 315 VDNSSV PE KSISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N+ +N + K S F E + E+P KLPP NSSSI DIQHLSNRSPVDN+SVIPETGE Sbjct: 242 VDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSNSERSILSEIPPKLPPINSSSI-DIQHLSNRSPVDNSSVIPETGE 374 HSP 4 Score: 108.997 bits (271), Expect = 8.201e-29 Identity = 73/108 (67.59%), Postives = 84/108 (77.78%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLS------NRSPVDNNSVIPE 306 VDNSSVFPEKSISNEFIE NQS+IIDSVLIN SQTSIRYLESDKTFVLSNAED +NIAD+SI E E+PS L +SSS+ +IQ LS N+S +D + +I E Sbjct: 695 VDNSSVFPEKSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAED-KNIADQSIINEISEIPSNLSSIDSSSV-EIQALSEFIENRNQSNIDGSVIINE 800 HSP 5 Score: 93.2041 bits (230), Expect = 2.164e-23 Identity = 55/86 (63.95%), Postives = 65/86 (75.58%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSS 240 VDNSSV PE KSISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N+++GQN+ADKSI E E+ KL +NS+ Sbjct: 461 VDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNENPEITYKLSRENST 546 HSP 6 Score: 67.781 bits (164), Expect = 1.788e-14 Identity = 39/48 (81.25%), Postives = 40/48 (83.33%), Query Frame = 1 Query: 175 ADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGEV 318 ADKSI E MPSKL P NSSSID IQHLSNRSPVDN+SVIPETGEV Sbjct: 210 ADKSIINE--NMPSKLHPINSSSID-IQHLSNRSPVDNSSVIPETGEV 254 HSP 7 Score: 62.003 bits (149), Expect = 2.477e-12 Identity = 41/73 (56.16%), Postives = 46/73 (63.01%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDG---QNIADKSIFTEYREM 210 VDNSSV PEK ISN FIE +NQSNI S+LINK + I L S+ N E G NIA+KSI EY EM Sbjct: 854 VDNSSVIPEKPISNRFIENQNQSNIDRSILINKLRLPIECLGSN------NDESGLENHNIANKSIINEYPEM 920 HSP 8 Score: 44.2838 bits (103), Expect = 3.857e-6 Identity = 41/91 (45.05%), Postives = 52/91 (57.14%), Query Frame = 1 Query: 43 EFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDG---QNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPE 306 EFIE RNQSNI SV+IN+ Q + S+ E G NIA+KSI E + S L +SS I +I L+ +S VDN+SVIPE Sbjct: 782 EFIENRNQSNIDGSVIINEFQLPMECSGSNTN------EPGLANHNIANKSIINENQ---SNLSSIDSSGI-EILALTEQSQVDNSSVIPE 862
BLAST of SMED30019370 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1) HSP 1 Score: 172.17 bits (435), Expect = 5.781e-51 Identity = 96/112 (85.71%), Postives = 97/112 (86.61%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGEV 318 VDNSSV PE KSISNEFIE NQSNI DSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSI EYREMPSKL P NSSSI DIQHLSNRSPVDN+SVIPETGEV Sbjct: 364 VDNSSVIPETGEFIEKSISNEFIENLNQSNINDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIINEYREMPSKLSPINSSSI-DIQHLSNRSPVDNSSVIPETGEV 474 HSP 2 Score: 132.88 bits (333), Expect = 2.720e-37 Identity = 75/102 (73.53%), Postives = 80/102 (78.43%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPE 306 V NS + PEKSISNEFIEYRNQSNIIDSVLINKSQTSIRY ESDKTFVLSNAEDGQNI+DKSI E +M SKL KN LSN+S VDN+SV PE Sbjct: 612 VKNSLLLPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYSESDKTFVLSNAEDGQNISDKSIINENSKMSSKLSHKN---------LSNKSQVDNSSVFPE 704 HSP 3 Score: 114.005 bits (284), Expect = 1.143e-30 Identity = 78/134 (58.21%), Postives = 87/134 (64.93%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQN--IADK-----SIFTEYR----------------EMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGE 315 VDNSSV PE KSISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N+ +N + K S F E + E+P KLPP NSSSI DIQHLSNRSPVDN+SVIPETGE Sbjct: 243 VDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSIPNENPEMLSKLPHTNSAFREMQSISLNENSNSERSILSEIPPKLPPINSSSI-DIQHLSNRSPVDNSSVIPETGE 375 HSP 4 Score: 108.997 bits (271), Expect = 8.281e-29 Identity = 73/108 (67.59%), Postives = 84/108 (77.78%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLS------NRSPVDNNSVIPE 306 VDNSSVFPEKSISNEFIE NQS+IIDSVLIN SQTSIRYLESDKTFVLSNAED +NIAD+SI E E+PS L +SSS+ +IQ LS N+S +D + +I E Sbjct: 696 VDNSSVFPEKSISNEFIENLNQSDIIDSVLINNSQTSIRYLESDKTFVLSNAED-KNIADQSIINEISEIPSNLSSIDSSSV-EIQALSEFIENRNQSNIDGSVIINE 801 HSP 5 Score: 93.2041 bits (230), Expect = 2.207e-23 Identity = 55/86 (63.95%), Postives = 65/86 (75.58%), Query Frame = 1 Query: 1 VDNSSVFPE------KSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDGQNIADKSIFTEYREMPSKLPPKNSS 240 VDNSSV PE KSISNEFIE RNQSNIIDS LINKSQ+S R +S+KTFVL N+++GQN+ADKSI E E+ KL +NS+ Sbjct: 462 VDNSSVIPETGEVIEKSISNEFIENRNQSNIIDSGLINKSQSSKRSFQSNKTFVLRNSDEGQNVADKSIPNENPEITYKLSRENST 547 HSP 6 Score: 67.781 bits (164), Expect = 1.805e-14 Identity = 39/48 (81.25%), Postives = 40/48 (83.33%), Query Frame = 1 Query: 175 ADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPETGEV 318 ADKSI E MPSKL P NSSSID IQHLSNRSPVDN+SVIPETGEV Sbjct: 211 ADKSIINE--NMPSKLHPINSSSID-IQHLSNRSPVDNSSVIPETGEV 255 HSP 7 Score: 62.003 bits (149), Expect = 2.477e-12 Identity = 41/73 (56.16%), Postives = 46/73 (63.01%), Query Frame = 1 Query: 1 VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDG---QNIADKSIFTEYREM 210 VDNSSV PEK ISN FIE +NQSNI S+LINK + I L S+ N E G NIA+KSI EY EM Sbjct: 855 VDNSSVIPEKPISNRFIENQNQSNIDRSILINKLRLPIECLGSN------NDESGLENHNIANKSIINEYPEM 921 HSP 8 Score: 44.2838 bits (103), Expect = 3.820e-6 Identity = 41/91 (45.05%), Postives = 52/91 (57.14%), Query Frame = 1 Query: 43 EFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSNAEDG---QNIADKSIFTEYREMPSKLPPKNSSSIDDIQHLSNRSPVDNNSVIPE 306 EFIE RNQSNI SV+IN+ Q + S+ E G NIA+KSI E + S L +SS I +I L+ +S VDN+SVIPE Sbjct: 783 EFIENRNQSNIDGSVIINEFQLPMECSGSNTN------EPGLANHNIANKSIINENQ---SNLSSIDSSGI-EILALTEQSQVDNSSVIPE 863 The following BLAST results are available for this feature:
BLAST of SMED30019370 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30019370 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30019370 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30019370 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30019370 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019370 ID=SMED30019370|Name=SMED30019370|organism=Schmidtea mediterranea sexual|type=transcript|length=320bpback to top protein sequence of SMED30019370-orf-1 >SMED30019370-orf-1 ID=SMED30019370-orf-1|Name=SMED30019370-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp VDNSSVFPEKSISNEFIEYRNQSNIIDSVLINKSQTSIRYLESDKTFVLSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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