SMED30019363

Overview


Name	SMED30019363
Smed ID	SMED30019363
Length (bp)	1407

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30019363 (SMED30019363) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019363 (SMED30019363) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019363

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
testis	SMED30019363	h1SMcG0008287	DN311232.1	smed_ncbi_20240424	PMID:22074376 Chong et al., 2011
spermatocyte	SMED30019363	h1SMcG0008287	DN311232.1	smed_ncbi_20240424	PMID:22074376 Chong et al., 2011
spermatid	SMED30019363	h1SMcG0008287	DN311232.1	smed_ncbi_20240424	PMID:22074376 Chong et al., 2011
testis	SMED30019363	h1SMcG0008287	HO005509.1	smed_ncbi_20240424	PMID:22074376 Chong et al., 2011
spermatocyte	SMED30019363	h1SMcG0008287	HO005509.1	smed_ncbi_20240424	PMID:22074376 Chong et al., 2011
spermatid	SMED30019363	h1SMcG0008287	HO005509.1	smed_ncbi_20240424	PMID:22074376 Chong et al., 2011

Note: Hover over icons to view figure legend

Alignments

SMED30019363 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.002060:51533..52939 +	7035
v31.005455:37014..38420 -	6954

Homology

[Close]

BLAST of SMED30019363 vs. Ensembl Human
Match: KLC4 (kinesin light chain 4 [Source:HGNC Symbol;Acc:HGNC:21624])

HSP 1 Score: 58.9214 bits (141), Expect = 1.200e-8
Identity = 83/327 (25.38%), Postives = 139/327 (42.51%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGE--DD----ELEENPSDSIK---------------P--LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTF 1173
            L  ++SE   +  +V+R+  E   + +E+   Q+  ++    +  +E EK++   + ++R     G  S+++    T +  DD    E EE+PS+ +                P  L  + N  IQ     R+E+A  L    L++L +    + + DVA++  ILA ++  +N Y  A       L IRE  L   HP +     N  +   K   +KEAE     ALEI++  LG  H  +A   N L  +     K      +Y+ AL I +  L  ++  +     +LA C  Y++     EA    E L+  I TR     F
Sbjct:   95 LSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSG-RGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASC--YLKQGKYAEA----ETLYKEILTRAHVQEF 414

[Close]

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGE--DD----ELEENPSDSIK---------------P--LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTF 1173
            L  ++SE   +  +V+R+  E   + +E+   Q+  ++    +  +E EK++   + ++R     G  S+++    T +  DD    E EE+PS+ +                P  L  + N  IQ     R+E+A  L    L++L +    + + DVA++  ILA ++  +N Y  A       L IRE  L   HP +     N  +   K   +KEAE     ALEI++  LG  H  +A   N L  +     K      +Y+ AL I +  L  ++  +     +LA C  Y++     EA    E L+  I TR     F
Sbjct:   95 LSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSG-RGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASC--YLKQGKYAEA----ETLYKEILTRAHVQEF 414

[Close]

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGE--DD----ELEENPSDSIK---------------P--LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTF 1173
            L  ++SE   +  +V+R+  E   + +E+   Q+  ++    +  +E EK++   + ++R     G  S+++    T +  DD    E EE+PS+ +                P  L  + N  IQ     R+E+A  L    L++L +    + + DVA++  ILA ++  +N Y  A       L IRE  L   HP +     N  +   K   +KEAE     ALEI++  LG  H  +A   N L  +     K      +Y+ AL I +  L  ++  +     +LA C  Y++     EA    E L+  I TR     F
Sbjct:   95 LSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSG-RGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASC--YLKQGKYAEA----ETLYKEILTRAHVQEF 414

[Close]

BLAST of SMED30019363 vs. Ensembl Human
Match: KLC4 (kinesin light chain 4 [Source:HGNC Symbol;Acc:HGNC:21624])

HSP 1 Score: 58.5362 bits (140), Expect = 1.568e-8
Identity = 83/327 (25.38%), Postives = 139/327 (42.51%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGE--DD----ELEENPSDSIK---------------P--LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTF 1173
            L  ++SE   +  +V+R+  E   + +E+   Q+  ++    +  +E EK++   + ++R     G  S+++    T +  DD    E EE+PS+ +                P  L  + N  IQ     R+E+A  L    L++L +    + + DVA++  ILA ++  +N Y  A       L IRE  L   HP +     N  +   K   +KEAE     ALEI++  LG  H  +A   N L  +     K      +Y+ AL I +  L  ++  +     +LA C  Y++     EA    E L+  I TR     F
Sbjct:  113 LSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSG-RGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASC--YLKQGKYAEA----ETLYKEILTRAHVQEF 432

[Close]

BLAST of SMED30019363 vs. Ensembl Human
Match: KLC2 (kinesin light chain 2 [Source:HGNC Symbol;Acc:HGNC:20716])

HSP 1 Score: 58.151 bits (139), Expect = 2.374e-8
Identity = 78/295 (26.44%), Postives = 133/295 (45.08%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGE-----ESKQEMKLNTGED---DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q  QK   +E    +LE+E  +++F M  I  L E     E K ++  +T +D   +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQ--QKLQRSEQAVAQLEEEKQHLLF-MSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 353

[Close]

BLAST of SMED30019363 vs. Ensembl Celegans
Match: klc-2 (Kinesin light chain [Source:UniProtKB/Swiss-Prot;Acc:P46822])

HSP 1 Score: 55.0694 bits (131), Expect = 7.895e-8
Identity = 37/116 (31.90%), Postives = 55/116 (47.41%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   FK+AE     ALEI++  LG +H  +A   N L  +     K      +Y+ AL I
Sbjct:  246 DVATMLNILALVYRDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALEIREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEI 361

[Close]

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   FK+AE     ALEI++  LG +H  +A   N L  +     K      +Y+ AL I
Sbjct:  246 DVATMLNILALVYRDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALEIREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEI 361

[Close]

BLAST of SMED30019363 vs. Ensembl Celegans
Match: klc-2 (Kinesin light chain [Source:UniProtKB/Swiss-Prot;Acc:P46822])

HSP 1 Score: 54.6842 bits (130), Expect = 8.696e-8
Identity = 37/116 (31.90%), Postives = 55/116 (47.41%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   FK+AE     ALEI++  LG +H  +A   N L  +     K      +Y+ AL I
Sbjct:  267 DVATMLNILALVYRDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALEIREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEI 382

[Close]

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   FK+AE     ALEI++  LG +H  +A   N L  +     K      +Y+ AL I
Sbjct:  267 DVATMLNILALVYRDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALEIREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEI 382

[Close]

BLAST of SMED30019363 vs. Ensembl Celegans
Match: klc-2 (Kinesin light chain [Source:UniProtKB/Swiss-Prot;Acc:P46822])

HSP 1 Score: 54.299 bits (129), Expect = 1.144e-7
Identity = 37/116 (31.90%), Postives = 55/116 (47.41%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   FK+AE     ALEI++  LG +H  +A   N L  +     K      +Y+ AL I
Sbjct:  246 DVATMLNILALVYRDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALEIREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEI 361

[Close]

BLAST of SMED30019363 vs. Ensembl Fly
Match: Klc (gene:FBgn0010235 transcript:FBtr0331556)

HSP 1 Score: 56.9954 bits (136), Expect = 2.242e-8
Identity = 57/209 (27.27%), Postives = 93/209 (44.50%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIG---FDTFQKKSCYIYFKRHLNDAKGEHLF-----TFYGSFSEY 1266
            DVA++  ILA ++  +N Y  A +     L IR + L + HP +     N  +   K   +K+AE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL+I ++ L  +   +     +LA C  Y++    TEA    E L+  + TR     F     K+  I+    + + + EH F     T YG +  +
Sbjct:  226 DVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGC--YLKQGRYTEA----EILYKQVLTRAHEREFGAIDSKNKPIW---QVAEEREEHKFDNRENTPYGEYGGW 425

[Close]

BLAST of SMED30019363 vs. Ensembl Fly
Match: Klc (gene:FBgn0010235 transcript:FBtr0075951)

HSP 1 Score: 56.9954 bits (136), Expect = 2.366e-8
Identity = 57/209 (27.27%), Postives = 93/209 (44.50%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIG---FDTFQKKSCYIYFKRHLNDAKGEHLF-----TFYGSFSEY 1266
            DVA++  ILA ++  +N Y  A +     L IR + L + HP +     N  +   K   +K+AE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL+I ++ L  +   +     +LA C  Y++    TEA    E L+  + TR     F     K+  I+    + + + EH F     T YG +  +
Sbjct:  226 DVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGC--YLKQGRYTEA----EILYKQVLTRAHEREFGAIDSKNKPIW---QVAEEREEHKFDNRENTPYGEYGGW 425

[Close]

BLAST of SMED30019363 vs. Ensembl Fly
Match: Klc (gene:FBgn0010235 transcript:FBtr0331555)

HSP 1 Score: 56.9954 bits (136), Expect = 2.366e-8
Identity = 57/209 (27.27%), Postives = 93/209 (44.50%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIG---FDTFQKKSCYIYFKRHLNDAKGEHLF-----TFYGSFSEY 1266
            DVA++  ILA ++  +N Y  A +     L IR + L + HP +     N  +   K   +K+AE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL+I ++ L  +   +     +LA C  Y++    TEA    E L+  + TR     F     K+  I+    + + + EH F     T YG +  +
Sbjct:  226 DVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGC--YLKQGRYTEA----EILYKQVLTRAHEREFGAIDSKNKPIW---QVAEEREEHKFDNRENTPYGEYGGW 425

[Close]

BLAST of SMED30019363 vs. Ensembl Zebrafish
Match: klc1a (kinesin light chain 1a [Source:ZFIN;Acc:ZDB-GENE-040426-1599])

HSP 1 Score: 55.0694 bits (131), Expect = 1.259e-7
Identity = 33/93 (35.48%), Postives = 48/93 (51.61%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFL 945
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L
Sbjct:  258 DVATMLNILALVYRDQNKYKEAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNL 350

[Close]

BLAST of SMED30019363 vs. Ensembl Zebrafish
Match: klc1a (kinesin light chain 1a [Source:ZFIN;Acc:ZDB-GENE-040426-1599])

HSP 1 Score: 55.0694 bits (131), Expect = 1.403e-7
Identity = 33/93 (35.48%), Postives = 48/93 (51.61%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFL 945
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L
Sbjct:  258 DVATMLNILALVYRDQNKYKEAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNL 350

[Close]

BLAST of SMED30019363 vs. Ensembl Zebrafish
Match: klc3 (kinesin light chain 3 [Source:ZFIN;Acc:ZDB-GENE-060929-772])

HSP 1 Score: 51.9878 bits (123), Expect = 1.060e-6
Identity = 37/116 (31.90%), Postives = 53/116 (45.69%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A       L IRE+ L   HP +     N  +   K   +KEAE     ALEI++  LG +H  +A   N L  +     K      +Y  AL+I
Sbjct:  234 DVATMLNILALVYRDQNKYKEAASLLNDALAIREKTLGMDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGTDHPDVAKQLNNLALLCQNQGKYQEVEQYYERALHI 349

[Close]

BLAST of SMED30019363 vs. Ensembl Zebrafish
Match: klc4 (kinesin light chain 4 [Source:ZFIN;Acc:ZDB-GENE-050417-350])

HSP 1 Score: 50.8322 bits (120), Expect = 2.861e-6
Identity = 32/94 (34.04%), Postives = 46/94 (48.94%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A       L IRE+ L   +P +     N  +   K   +KEAE     ALEI++  LG+EH  +A   N L 
Sbjct:  247 DVATMLNILALVYRDQNKYKEAAHLLNDALSIREKTLGNDNPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGREHPDVAKQLNNLA 340

[Close]

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A       L IRE+ L   +P +     N  +   K   +KEAE     ALEI++  LG+EH  +A   N L 
Sbjct:  247 DVATMLNILALVYRDQNKYKEAAHLLNDALSIREKTLGNDNPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGREHPDVAKQLNNLA 340

[Close]

BLAST of SMED30019363 vs. Ensembl Xenopus
Match: hira (histone cell cycle regulator [Source:Xenbase;Acc:XB-GENE-976072])

HSP 1 Score: 55.8398 bits (133), Expect = 9.752e-8
Identity = 33/94 (35.11%), Postives = 48/94 (51.06%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   ++EAE     ALEI++  LGK+H  +A   N L 
Sbjct:  246 DVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYREAEPLCKRALEIREKVLGKDHPDVAKQLNNLA 339

[Close]

BLAST of SMED30019363 vs. Ensembl Xenopus
Match: hira (histone cell cycle regulator [Source:Xenbase;Acc:XB-GENE-976072])

HSP 1 Score: 55.8398 bits (133), Expect = 9.765e-8
Identity = 33/94 (35.11%), Postives = 48/94 (51.06%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   ++EAE     ALEI++  LGK+H  +A   N L 
Sbjct:  249 DVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYREAEPLCKRALEIREKVLGKDHPDVAKQLNNLA 342

[Close]

BLAST of SMED30019363 vs. Ensembl Xenopus
Match: hira (histone cell cycle regulator [Source:Xenbase;Acc:XB-GENE-976072])

HSP 1 Score: 55.8398 bits (133), Expect = 9.841e-8
Identity = 33/94 (35.11%), Postives = 48/94 (51.06%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   ++EAE     ALEI++  LGK+H  +A   N L 
Sbjct:  253 DVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYREAEPLCKRALEIREKVLGKDHPDVAKQLNNLA 346

[Close]

BLAST of SMED30019363 vs. Ensembl Xenopus
Match: hira (histone cell cycle regulator [Source:Xenbase;Acc:XB-GENE-976072])

HSP 1 Score: 55.8398 bits (133), Expect = 1.052e-7
Identity = 33/94 (35.11%), Postives = 48/94 (51.06%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   ++EAE     ALEI++  LGK+H  +A   N L 
Sbjct:  249 DVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYREAEPLCKRALEIREKVLGKDHPDVAKQLNNLA 342

[Close]

BLAST of SMED30019363 vs. Ensembl Xenopus
Match: hira (histone cell cycle regulator [Source:Xenbase;Acc:XB-GENE-976072])

HSP 1 Score: 55.8398 bits (133), Expect = 1.068e-7
Identity = 33/94 (35.11%), Postives = 48/94 (51.06%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   ++EAE     ALEI++  LGK+H  +A   N L 
Sbjct:  253 DVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYREAEPLCKRALEIREKVLGKDHPDVAKQLNNLA 346

[Close]

BLAST of SMED30019363 vs. Ensembl Mouse
Match: Klc2 (kinesin light chain 2 [Source:MGI Symbol;Acc:MGI:107953])

HSP 1 Score: 58.151 bits (139), Expect = 1.390e-8
Identity = 78/295 (26.44%), Postives = 132/295 (44.75%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEE-SKQEMKLNTGED-------DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q  QK   +E    +LE+E  +++F M  I  L E+ S  E K +  +D       +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQ--QKLQRSEQAVAQLEEEKQHLLF-MSQIRKLDEDASPNEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 353

[Close]

BLAST of SMED30019363 vs. Ensembl Mouse
Match: Klc2 (kinesin light chain 2 [Source:MGI Symbol;Acc:MGI:107953])

HSP 1 Score: 58.151 bits (139), Expect = 1.400e-8
Identity = 78/295 (26.44%), Postives = 132/295 (44.75%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEE-SKQEMKLNTGED-------DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q  QK   +E    +LE+E  +++F M  I  L E+ S  E K +  +D       +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQ--QKLQRSEQAVAQLEEEKQHLLF-MSQIRKLDEDASPNEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 353

[Close]

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEE-SKQEMKLNTGED-------DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q  QK   +E    +LE+E  +++F M  I  L E+ S  E K +  +D       +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQ--QKLQRSEQAVAQLEEEKQHLLF-MSQIRKLDEDASPNEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 353

[Close]

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEE-SKQEMKLNTGED-------DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q  QK   +E    +LE+E  +++F M  I  L E+ S  E K +  +D       +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQ--QKLQRSEQAVAQLEEEKQHLLF-MSQIRKLDEDASPNEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 353

[Close]

BLAST of SMED30019363 vs. Ensembl Mouse
Match: Klc1 (kinesin light chain 1 [Source:MGI Symbol;Acc:MGI:107978])

HSP 1 Score: 56.225 bits (134), Expect = 5.110e-8
Identity = 38/116 (32.76%), Postives = 56/116 (48.28%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL I
Sbjct:  253 DVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALGI 368

[Close]

BLAST of SMED30019363 vs. UniProt/SwissProt
Match: sp|O88448|KLC2_MOUSE (Kinesin light chain 2 OS=Mus musculus OX=10090 GN=Klc2 PE=1 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 7.648e-9
Identity = 71/291 (24.40%), Postives = 135/291 (46.39%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNI-TLLGEESKQEMKLNTGED---DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q++ ++    +  +E EK++   + ++R +  +L +E K ++  ++ +D   +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEMLPQEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 352

[Close]

BLAST of SMED30019363 vs. UniProt/SwissProt
Match: sp|Q9NSK0|KLC4_HUMAN (Kinesin light chain 4 OS=Homo sapiens OX=9606 GN=KLC4 PE=1 SV=3)

HSP 1 Score: 58.9214 bits (141), Expect = 5.761e-8
Identity = 83/327 (25.38%), Postives = 139/327 (42.51%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGE--DD----ELEENPSDSIK---------------P--LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTF 1173
            L  ++SE   +  +V+R+  E   + +E+   Q+  ++    +  +E EK++   + ++R     G  S+++    T +  DD    E EE+PS+ +                P  L  + N  IQ     R+E+A  L    L++L +    + + DVA++  ILA ++  +N Y  A       L IRE  L   HP +     N  +   K   +KEAE     ALEI++  LG  H  +A   N L  +     K      +Y+ AL I +  L  ++  +     +LA C  Y++     EA    E L+  I TR     F
Sbjct:   95 LSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSG-RGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYEGQLGPDNPNVARTKNNLASC--YLKQGKYAEA----ETLYKEILTRAHVQEF 414

[Close]

BLAST of SMED30019363 vs. UniProt/SwissProt
Match: sp|Q2HJJ0|KLC4_BOVIN (Kinesin light chain 4 OS=Bos taurus OX=9913 GN=KLC4 PE=2 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 5.846e-8
Identity = 82/324 (25.31%), Postives = 139/324 (42.90%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLG---EESKQEMKLNTGED---DELEENPSDSIK------------P--LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTF 1173
            L  ++SE   +  +V+R+  E   + +E+   Q+  ++    +  +E EK++   + ++R     G   EE + +   ++ +D   +E EE+PS+ +             P  L  + N  IQ     R+E+A  L    L++L +       + VA++  ILA ++  +N Y  A       L IRE  L + HP +     N  +   K   +KEAE     ALEI++  LG  H  +A   N L  +     K      +YR AL I +  L  ++  +     +LA C  Y++     EA    E L+  I TR     F
Sbjct:   95 LSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHAAEEKEGDASKDSLDDLFPNEEEEDPSNGLSRGQGAQHSGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPV-VATMLNILALVYRGQNKYKEAALLLNDALSIRESTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALEIREKVLGTNHPDVAKQLNNLALLCQNQGKYEAVERYYRRALAIYEGQLGPDNPNVARTKNNLASC--YLKQGKYAEA----ETLYKEILTRAHVQEF 411

[Close]

BLAST of SMED30019363 vs. UniProt/SwissProt
Match: sp|Q9H0B6|KLC2_HUMAN (Kinesin light chain 2 OS=Homo sapiens OX=9606 GN=KLC2 PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.140e-7
Identity = 78/295 (26.44%), Postives = 133/295 (45.08%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGE-----ESKQEMKLNTGED---DELEENPSDS------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVN---FYRLALNI 1014
            + I+    + ++I   +  L  ++SE   +  +V+R++ E   +  E+   Q  QK   +E    +LE+E  +++F M  I  L E     E K ++  +T +D   +E E++P+ S                  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A     L  + +L   Q  A     +YR AL I
Sbjct:   66 EAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQ--QKLQRSEQAVAQLEEEKQHLLF-MSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVA---KQLSNLALLCQNQGKAEEVEYYYRRALEI 353

[Close]

BLAST of SMED30019363 vs. UniProt/SwissProt
Match: sp|P46824|KLC_DROME (Kinesin light chain OS=Drosophila melanogaster OX=7227 GN=Klc PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 2.372e-7
Identity = 57/209 (27.27%), Postives = 93/209 (44.50%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIG---FDTFQKKSCYIYFKRHLNDAKGEHLF-----TFYGSFSEY 1266
            DVA++  ILA ++  +N Y  A +     L IR + L + HP +     N  +   K   +K+AE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL+I ++ L  +   +     +LA C  Y++    TEA    E L+  + TR     F     K+  I+    + + + EH F     T YG +  +
Sbjct:  226 DVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGC--YLKQGRYTEA----EILYKQVLTRAHEREFGAIDSKNKPIW---QVAEEREEHKFDNRENTPYGEYGGW 425

[Close]

BLAST of SMED30019363 vs. TrEMBL
Match: A0A498MX94 (Kinesin light chain 2 OS=Labeo rohita OX=84645 GN=ROHU_006950 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.378e-9
Identity = 70/262 (26.72%), Postives = 117/262 (44.66%), Query Frame = 1

Query:  247 EIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEENPSDSIKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN 1023
            ++I   +  L  ++SE   +  +V+R+  E   + +E+ S Q + +K    +  +E EK   E+ N + ++ + T   EE   +            E P+  ++ L  +  ++    R+E+A  L    L++L K      + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K    KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I +N
Sbjct:   98 QVIIALSGHLSAVESEKQKLRAQVRRLCQENQWLRDELASTQHKLQKSEQSVAQLEEEKKHLEFMNQIRKLDDDTSPSEEKAAQPSGEVAAQQGGYEIPA-RLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSG-HDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKHKEAEPLCKRALEIREKVLGKFHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYEN 357

[Close]

BLAST of SMED30019363 vs. TrEMBL
Match: A0A498MI54 (Kinesin light chain 2 OS=Labeo rohita OX=84645 GN=ROHU_007515 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.382e-9
Identity = 70/262 (26.72%), Postives = 117/262 (44.66%), Query Frame = 1

Query:  247 EIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEENPSDSIKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN 1023
            ++I   +  L  ++SE   +  +V+R+  E   + +E+ S Q + +K    +  +E EK   E+ N + ++ + T   EE   +            E P+  ++ L  +  ++    R+E+A  L    L++L K      + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K    KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I +N
Sbjct:   98 QVIIALSGHLSAVESEKQKLRAQVRRLCQENQWLRDELASTQHKLQKSEQSVAQLEEEKKHLEFMNQIRKLDDDTSPSEEKAAQPSGEVAAQQGGYEIPA-RLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSG-HDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKHKEAEPLCKRALEIREKVLGKFHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYEN 357

[Close]

BLAST of SMED30019363 vs. TrEMBL
Match: A0A067QTL0 (Kinesin light chain (Fragment) OS=Zootermopsis nevadensis OX=136037 GN=L798_13039 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.637e-9
Identity = 80/325 (24.62%), Postives = 145/325 (44.62%), Query Frame = 1

Query:  202 VQTISDKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMR--NITLLGEESKQEMKLNTGE----------DDELEENPSDSIKP-----------------LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            VQ   D ID    + +++      L+ +++E   +  +V+R+  E   + +E+ + Q++ +     +  +E +K+++  +  MR  +  + G++S  E K +  +          DD+ EE  + S  P                 L  + N  IQ     R+E+A  L    L++L K   +  + DVA++  ILA +++V+N Y  A +     L IRE+ L + HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL I ++ L  +   +     +LA C
Sbjct:   60 VQKSLDMIDLGLGEAQVMMALASHLQIVEAEKQKLRTQVRRLCQENAWLRDELANTQQKLQASEQAVAQLEEDKKHFEFMASMRKYDQDITGDDSSTETKQDKPKQDDPVVDLFPDDDTEERSTMSPTPPSQFAQQVNAGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYSVQNKYKEAANLLNDALAIREKTLGENHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVERYYQRALEIYESKLGPDDPNVAKTKNNLASC 383

[Close]

BLAST of SMED30019363 vs. TrEMBL
Match: A0A553Q0D6 (TPR_REGION domain-containing protein (Fragment) OS=Danionella translucida OX=623744 GN=DNTS_015074 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 3.987e-9
Identity = 78/310 (25.16%), Postives = 134/310 (43.23%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMKLNT------GEDDELEENPSDSI-------------KPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            D I+    + ++I   +  L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + ++   T   EE   E + ++       +DD+    PS  +             + L  +  ++    R+E+A  L    L++L K      + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K    KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I +N L ++   +     +LA C
Sbjct:  944 DDIELGLGEAQVIIALSTHLSAVESEKQKLKAQVRRLCQENQWLRDELANTQHKLQKSEQSVAQLEEEKKHLEFMNQIRKLDEDTSPSEEKPGEKEKDSLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSG-HDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKHKEAEPLCKRALEIREKVLGKFHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYENKLGSDDPNVAKTKNNLATC 1252

[Close]

BLAST of SMED30019363 vs. TrEMBL
Match: A0A553Q0A0 (TPR_REGION domain-containing protein (Fragment) OS=Danionella translucida OX=623744 GN=DNTS_015074 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 3.993e-9
Identity = 78/310 (25.16%), Postives = 134/310 (43.23%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMKLNT------GEDDELEENPSDSI-------------KPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            D I+    + ++I   +  L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + ++   T   EE   E + ++       +DD+    PS  +             + L  +  ++    R+E+A  L    L++L K      + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K    KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I +N L ++   +     +LA C
Sbjct:  954 DDIELGLGEAQVIIALSTHLSAVESEKQKLKAQVRRLCQENQWLRDELANTQHKLQKSEQSVAQLEEEKKHLEFMNQIRKLDEDTSPSEEKPGEKEKDSLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSG-HDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKHKEAEPLCKRALEIREKVLGKFHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYENKLGSDDPNVAKTKNNLATC 1262

[Close]

BLAST of SMED30019363 vs. Ensembl Cavefish
Match: ENSAMXT00000055945.1 (kinesin light chain 2-like [Source:NCBI gene;Acc:111194328])

HSP 1 Score: 61.6178 bits (148), Expect = 8.515e-10
Identity = 77/291 (26.46%), Postives = 125/291 (42.96%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMK------LNTGEDDELEENPSDSIKP----------LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + +  +     EE   E +      L   +DD+    PS  +            L  + N  IQ     R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I ++ L  +   +     +LA C
Sbjct:   85 LSAVESEKQKLRAQVRRLCQENQWLRDELANTQHKLQKSEQNVAQLEEEKKHLEFMNQIRKFDDDASPSEEKPGEKEKDNLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYESKLGADDPNVAKTKNNLATC 374

[Close]

BLAST of SMED30019363 vs. Ensembl Cavefish
Match: ENSAMXT00000055398.1 (kinesin light chain 2-like [Source:NCBI gene;Acc:111194328])

HSP 1 Score: 61.6178 bits (148), Expect = 8.515e-10
Identity = 77/291 (26.46%), Postives = 125/291 (42.96%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMK------LNTGEDDELEENPSDSIKP----------LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + +  +     EE   E +      L   +DD+    PS  +            L  + N  IQ     R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I ++ L  +   +     +LA C
Sbjct:   85 LSAVESEKQKLRAQVRRLCQENQWLRDELANTQHKLQKSEQNVAQLEEEKKHLEFMNQIRKFDDDASPSEEKPGEKEKDNLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYESKLGADDPNVAKTKNNLATC 374

[Close]

BLAST of SMED30019363 vs. Ensembl Cavefish
Match: ENSAMXT00000032282.1 (kinesin light chain 2-like [Source:NCBI gene;Acc:111194328])

HSP 1 Score: 61.6178 bits (148), Expect = 8.515e-10
Identity = 77/291 (26.46%), Postives = 125/291 (42.96%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMK------LNTGEDDELEENPSDSIKP----------LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + +  +     EE   E +      L   +DD+    PS  +            L  + N  IQ     R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I ++ L  +   +     +LA C
Sbjct:   85 LSAVESEKQKLRAQVRRLCQENQWLRDELANTQHKLQKSEQNVAQLEEEKKHLEFMNQIRKFDDDASPSEEKPGEKEKDNLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYESKLGADDPNVAKTKNNLATC 374

[Close]

BLAST of SMED30019363 vs. Ensembl Cavefish
Match: ENSAMXT00000031936.1 (kinesin light chain 2-like [Source:NCBI gene;Acc:111194328])

HSP 1 Score: 61.6178 bits (148), Expect = 8.515e-10
Identity = 77/291 (26.46%), Postives = 125/291 (42.96%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMK------LNTGEDDELEENPSDSIKP----------LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + +  +     EE   E +      L   +DD+    PS  +            L  + N  IQ     R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I ++ L  +   +     +LA C
Sbjct:   85 LSAVESEKQKLRAQVRRLCQENQWLRDELANTQHKLQKSEQNVAQLEEEKKHLEFMNQIRKFDDDASPSEEKPGEKEKDNLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYESKLGADDPNVAKTKNNLATC 374

[Close]

BLAST of SMED30019363 vs. Ensembl Cavefish
Match: ENSAMXT00000044075.1 (kinesin light chain 2-like [Source:NCBI gene;Acc:111194328])

HSP 1 Score: 61.6178 bits (148), Expect = 8.818e-10
Identity = 77/291 (26.46%), Postives = 125/291 (42.96%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMK------LNTGEDDELEENPSDSIKP----------LLIICNEFIQ---DNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAIC 1077
            L  ++SE   +  +V+R+  E   + +E+ + Q + +K    +  +E EK   E+ N + +  +     EE   E +      L   +DD+    PS  +            L  + N  IQ     R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A   N L  +     K      +YR AL I ++ L  +   +     +LA C
Sbjct:   85 LSAVESEKQKLRAQVRRLCQENQWLRDELANTQHKLQKSEQNVAQLEEEKKHLEFMNQIRKFDDDASPSEEKPGEKEKDNLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYESKLGADDPNVAKTKNNLATC 374

[Close]

BLAST of SMED30019363 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009139.1 (pep scaffold:Pmarinus_7.0:GL480151:1445:19017:1 gene:ENSPMAG00000008222.1 transcript:ENSPMAT00000009139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.077 bits (144), Expect = 6.253e-10
Identity = 45/138 (32.61%), Postives = 66/138 (47.83%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI-LDNLSTESFELTYLYISLAIC 1077
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L  +     K      +YR AL+I L  L T+   +     +LA C
Sbjct:  250 DVATMLNILALVYRDQNKYKEAGNLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVETYYRRALDIYLLQLGTDDPNVAKTKNNLASC 387

[Close]

BLAST of SMED30019363 vs. Ensembl Sea Lamprey
Match: klc1a (kinesin light chain 1a [Source:ZFIN;Acc:ZDB-GENE-040426-1599])

HSP 1 Score: 60.077 bits (144), Expect = 6.316e-10
Identity = 90/372 (24.19%), Postives = 160/372 (43.01%), Query Frame = 1

Query:  265 TKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMR----NITLLGEESKQEM------KLNTGEDDELEENPSDS---------------------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDN-LSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRI-----GFDTFQKKSCYIYFKRHLNDAKGEH----LFTFYGSF 1257
            T  L  ++SE   +  +V+R+  E   + +E+ + Q++ ++    +  +E EK++   + +M+    +I+   EE   E        L   E+DE E+    S                     ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK H  +A   N L  +    +K     ++Y+ AL+I  + L  +   +     +LA C  Y++     EA    E L+  I TR      G    + K  +++ +    + KG+H     +  YGS+
Sbjct:   47 TSHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQRSEQAVAQLEEEKKHLEFMNQMKKYDDDISPSQEEKVSEPCKEPLDDLFPNEEDEQEQGSQPSHNAVNSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAASLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKVHPDVAKQLNNLALLCQNQSKYEEVEHYYQRALDIYQSQLGPDDPNVAKTKNNLASC--YLKQGKYKEA----EGLYKDILTRAHEKEFGSVDGENKPIWMHAEER-EEQKGKHKESPPYGEYGSW 410

[Close]

BLAST of SMED30019363 vs. Ensembl Nematostella
Match: EDO43009 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZV0])

HSP 1 Score: 53.9138 bits (128), Expect = 8.552e-8
Identity = 37/116 (31.90%), Postives = 56/116 (48.28%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI 1014
            DVA++  ILA ++  +N Y  A +     L IRE+ L + HP +     N  +   K   +K+AE     ALEI++  LGK+H  +A   N L  +     K      +Y+ AL I
Sbjct:  236 DVATMLNILALVYRDQNKYKEAANLLHDALTIREKTLGEDHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEQYYQRALEI 351

[Close]

BLAST of SMED30019363 vs. Ensembl Medaka
Match: ENSORLT00000016251.2 (kinesin light chain 1 [Source:NCBI gene;Acc:101171520])

HSP 1 Score: 58.9214 bits (141), Expect = 7.216e-9
Identity = 64/255 (25.10%), Postives = 117/255 (45.88%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMR----NITLLGEESKQEMKLNTGED---DELEEN-----------------------PSDSIKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            L  ++SE   +  +V+R+  E   + +E+ + Q++ +K    +  +E EK++   + +++    +++   EE  +E   ++ +D   +E EEN                       P+  ++ L  +  ++    R+E+A  L    L++L K   +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGKEH  +A   N L 
Sbjct:   92 LNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQNVAQLEEEKKHLEFMNQLKKYDEDVSPTQEEKDRETPKDSLDDLFPNEDEENNQGMQHQHNSAAVAAAQQGGYEIPA-RLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALSIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKEHPDVAKQLNNLA 344

[Close]

BLAST of SMED30019363 vs. Ensembl Medaka
Match: klc1a (kinesin light chain 1 [Source:NCBI gene;Acc:101163537])

HSP 1 Score: 55.8398 bits (133), Expect = 5.967e-8
Identity = 34/93 (36.56%), Postives = 48/93 (51.61%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFL 945
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L
Sbjct:  254 DVATMLNILALVYRDQNKYKEAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNL 346

[Close]

BLAST of SMED30019363 vs. Ensembl Medaka
Match: klc1a (kinesin light chain 1 [Source:NCBI gene;Acc:101163537])

HSP 1 Score: 55.8398 bits (133), Expect = 6.350e-8
Identity = 34/94 (36.17%), Postives = 48/94 (51.06%), Query Frame = 1

Query:  667 DVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLG 948
            DVA++  ILA ++  +N Y  A +     L IRE+ L K HP +     N  +   K   +KEAE     ALEI++  LGK+H  +A   N L 
Sbjct:  254 DVATMLNILALVYRDQNKYKEAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLA 347

[Close]

BLAST of SMED30019363 vs. Ensembl Medaka
Match: ENSORLT00000010217.2 (kinesin light chain 2 [Source:NCBI gene;Acc:101159385])

HSP 1 Score: 54.6842 bits (130), Expect = 1.315e-7
Identity = 74/302 (24.50%), Postives = 122/302 (40.40%), Query Frame = 1

Query:  274 LRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMKLNTG---------------EDDELEENPSDSIK--------PL-------LIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI-LDNLSTESFELTYLYISLAIC 1077
            L  ++SE   +  +V+R+  E   + +E+ + Q +       +  +E EK   E+ N + +  +     EE  Q      G               +DD+    PS  +         P+       L+I  ++    R+E+A  L    L++L K+  +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI+   LGK H  +A   N L  +     K      +Y  AL I +  L  +   +     +LA C
Sbjct:   85 LSAVESEKQKLRAQVRRLCQENQWLRDELAATQHKLHCSEQSVAQLEEEKKHLEFMNQIKKFDDDASPAEEKNQSCGDGAGAGEASKDSLDDLFPNDDDQGPAQPSGDVAAQHAGYEIPVRLRTLHNLVI--QYASQGRYEVAVPLCKQALEDLEKSSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIRVKVLGKLHPDVAKQLNNLALLCQNQGKYDEVEYYYTQALEIYVSQLGPDDPNVAKTKNNLATC 383

[Close]

BLAST of SMED30019363 vs. Ensembl Medaka
Match: ENSORLT00000010213.2 (kinesin light chain 2 [Source:NCBI gene;Acc:101159385])

HSP 1 Score: 54.299 bits (129), Expect = 1.967e-7
Identity = 75/313 (23.96%), Postives = 127/313 (40.58%), Query Frame = 1

Query:  241 DYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEK---EYWNVVFRMRNITLLGEESKQEMKLNTG---------------EDDELEENPSDSIK--------PL-------LIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNI-LDNLSTESFELTYLYISLAIC 1077
            + ++I   +  L  ++SE   +  +V+R+  E   + +E+ + Q +       +  +E EK   E+ N + +  +     EE  Q      G               +DD+    PS  +         P+       L+I  ++    R+E+A  L    L++L K+  +  + DVA++  ILA ++  +N Y  A       L IRE+ L K HP +     N  +   K   +KEAE     ALEI+   LGK H  +A   N L  +     K      +Y  AL I +  L  +   +     +LA C
Sbjct:   74 EAQVIIALSGHLSAVESEKQKLRAQVRRLCQENQWLRDELAATQHKLHCSEQSVAQLEEEKKHLEFMNQIKKFDDDASPAEEKNQSCGDGAGAGEASKDSLDDLFPNDDDQGPAQPSGDVAAQHAGYEIPVRLRTLHNLVI--QYASQGRYEVAVPLCKQALEDLEKSSGHD-HPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIRVKVLGKLHPDVAKQLNNLALLCQNQGKYDEVEYYYTQALEIYVSQLGPDDPNVAKTKNNLATC 383

[Close]

BLAST of SMED30019363 vs. Planmine SMEST
Match: SMESG000076903.1 (SMESG000076903.1)

HSP 1 Score: 878.241 bits (2268), Expect = 0.000e+0
Identity = 444/446 (99.55%), Postives = 445/446 (99.78%), Query Frame = 1

Query:   31 MALVRGFEGNNMLPLLLYLERFFNQYSEYFVNEKNRVTQNKPRDSLGSRIHLLSLNYVQTISDKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEENPSDSIKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDNLSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTFQKKSCYIYFKRHLNDAKGEHLFTFYGSFSEYDILDLFEILVKHFKNNPKVIEAIDIWKYLSIQST 1368
            MALVRGFEGNNMLPLLLYLERFF QYSEYFVNEKNRVTQNKPRDSLGSRIHLLSLNYVQTISDKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRIL+EETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEENPSDSIKPLLIICNEFIQDNRFEMAEGLATDKLKELLKNLKNQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDNLSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTFQKKSCYIYFKRHLNDAKGEHLFTFYGSFSEYDILDLFEILVKHFKNNPKVIEAIDIWKYLSIQST
Sbjct:    1 MALVRGFEGNNMLPLLLYLERFFTQYSEYFVNEKNRVTQNKPRDSLGSRIHLLSLNYVQTISDKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILSEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEENPSDSIKPLLIICNEFIQDNRFEMAEGLATDKLKELLKNLKNQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDNLSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTFQKKSCYIYFKRHLNDAKGEHLFTFYGSFSEYDILDLFEILVKHFKNNPKVIEAIDIWKYLSIQST 446

[Close]

BLAST of SMED30019363 vs. Planmine SMEST
Match: SMESG000010480.1 (SMESG000010480.1)

HSP 1 Score: 109.768 bits (273), Expect = 1.397e-25
Identity = 102/422 (24.17%), Postives = 201/422 (47.63%), Query Frame = 1

Query:  175 RIHLLSLNYVQTISD----KIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEEN-----------------PSDSIKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDNL--STESFELTYLYISLAICRIYVEMKISTEADNHLEKLFNLIETRIGFDTFQKKSCYIYFKRHLNDAKG-------EHLFTFYGSFSEYDILDLFEILVKHFKN--NPKVIEAIDIW 1344
            ++  L L+  Q I+D    K++    + E++         +D+E     ++V+R++ E   + +E+  +QK   KL   +   E EK+++  V++MRN                  DDE   N                 P+  +K +  +C E+I++ R+E+A  L  + + ++     +  N D+A++Y+I+AK+H  + +   A  +++ CL++RE++L K H  +G    +      K  + KEAEK  T AL+I++   GK    IA+I++ LG      +K    +N Y++AL I D +  +T+S  +   + ++A    YV++K   + +N  E L  + ++    ++    +    F+  L+ A+G       E+L   YG++      D+ E ++++F N   P   E++ +W
Sbjct:   50 KVSQLILSEKQWITDRTVEKLELGADEIEVVLTLNSFCCIMDAEKVRTRLQVQRLVQENKWLRSELIDLQKRLMKLEESVVAAEAEKQHFQFVYKMRN---------------QQRDDEQIYNYLIDAEDEEDTDPEDEIPA-KVKAVHNLCMEYIREKRYEVAIPLCEEHIHQIKNKQGDNNN-DIATLYSIIAKVHKEQGNLAEAQKSYEDCLKLREKVLGKEHNAVGVTLTSLAQVHYKRNNVKEAEKLATQALKIREKSAGKNTAEIALIHSHLGMYACQLSKWNDVINHYKVALGIYDQMYDNTDSL-IIKAHSNMA--NAYVKLK---QNENACEMLSLVCKSFQQKNSNTSDNSLTIFQTALHHAEGKLADLNAEYLMKTYGAYENAIDKDVLETIIEYFNNVKKPATAESLKVW 448

[Close]

BLAST of SMED30019363 vs. Planmine SMEST
Match: SMESG000043408.1 (SMESG000043408.1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.785e-7
Identity = 74/316 (23.42%), Postives = 140/316 (44.30%), Query Frame = 1

Query:  202 VQTISDKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQE-----MKLNT--------GE----DDELEE----------NPSDS--------IKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDNLSTESFE 1044
            +Q  +DK++    D  +++  ++ L+ ID+E    ++ + ++  E   +  E+ ++Q   +K   +I  +E E ++ + +  +++I  +G ++ QE      +LN         GE    D E E           NPS++        +K L  +  ++    R+E+A  L    L++L +      + DVA++  ILA ++  +N +P A+   K  L IRE  L   H  +     N  +   K K F +AE     +L I++  LG +H  +A   N L  +    NK     ++Y  A+ I  N   +  E
Sbjct:   51 IQHSTDKVNLAIEDANVLKLLSEYLKVIDAERQNHSVNIIKLNEESNWLREEVTNLQSNLRKSEEKIVELEEENKHLHFLLELKSIDDMGADTGQEKSKDLFELNKLDNTNPSDGEFKFPDTEFENKESPNAENVVNPSNASGFELPPRLKTLHNLVIQYASQGRYEVAIPLCKQTLEDLERTSGRN-HPDVATMLNILALVYRDQNKFPDAITLLKEALAIRENTLGPVHVAVAANLNNLAVLYGKQKKFNDAEPLCKRSLIIREQLLGPDHPDVAKQLNNLALLCQNQNKYSEVESYYLRAIKIYTNAYGQQHE 365

[Close]

BLAST of SMED30019363 vs. Planmine SMEST
Match: SMESG000007554.1 (SMESG000007554.1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.309e-6
Identity = 26/89 (29.21%), Postives = 44/89 (49.44%), Query Frame = 1

Query:  661 NLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIA 927
            +LD+A++  I A ++  + +Y  A++     L +RE LL   HP I     N  +   ++   +EAE     AL I++  LG  H  +A
Sbjct:  266 SLDIATLQNIFALVYRDQGNYAEAVNLLNKSLTVRENLLGTNHPLIAITLNNIAILQARIGKLREAENMCRRALAIREQHLGSNHPDVA 354

[Close]

BLAST of SMED30019363 vs. Planmine SMEST
Match: SMESG000042588.1 (SMESG000042588.1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.457e-6
Identity = 70/314 (22.29%), Postives = 142/314 (45.22%), Query Frame = 1

Query:  217 DKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRILNEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEESKQEMKLNTGEDDELEENPS------DSIKPLLIICNEFIQDNRFEMAEGLATDXXXXXXXXXXXQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLDKFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAMIYNFLGKINVLTNKQIVAVNFYRLALNILDNLSTESFELTYLYISLAICRIYVEMKISTEADNHLEKLFN 1140
            + I   + + E+I    K L  +D E   + + VK IL E   + +E++ +Q    K+  +    E EK+++   F MRN      +S   ++  T  +D  +E+         +++ L  + ++FI ++R  +A      K     +    + + +V ++Y +L +++     Y  A + F+  L I +++  K    +G         L + K +KEA      AL I++N   K    I+ +   + + ++  +    +V +Y++ALN LD +  ++ E+  +     + R YVE  I  +A   LE++ N
Sbjct:   65 ESIAVAESELELISHVAKYLLILDLERGKVKLHVKYILKENLWLKSEMYDLQGILMKVEHQFLLAEAEKKHYQYYFNMRNY-----QSTNVVRPETAIEDLDDEDTDPEEEILSNMRALFTVVHQFISESRHTVAIAWC-QKSIGDCQKKYGEMSAEVGAIYNLLGRVYKDFEYYIIAQECFEKALFINQKVYGKNSDQVGITMSLLASILGRRKVYKEALALCKQALTIRENVNNKNSVDISRLQLKIAEYSLELHSWNESVTYYKMALNHLDTVFDQT-EILIIKSYTNLARAYVETNILNKAAECLERVNN 371

The following BLAST results are available for this feature:

BLAST of SMED30019363 vs. Ensembl Human
BLAST of SMED30019363 vs. Ensembl Celegans
BLAST of SMED30019363 vs. Ensembl Fly
BLAST of SMED30019363 vs. Ensembl Zebrafish
BLAST of SMED30019363 vs. Ensembl Xenopus
BLAST of SMED30019363 vs. Ensembl Mouse
BLAST of SMED30019363 vs. UniProt/SwissProt
BLAST of SMED30019363 vs. TrEMBL
BLAST of SMED30019363 vs. Ensembl Cavefish
BLAST of SMED30019363 vs. Ensembl Sea Lamprey
BLAST of SMED30019363 vs. Ensembl Yeast
BLAST of SMED30019363 vs. Ensembl Nematostella
BLAST of SMED30019363 vs. Ensembl Medaka
BLAST of SMED30019363 vs. Planmine SMEST

BLAST of SMED30019363 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
KLC4	1.200e-8	25.38	kinesin light chain 4 [Source:HGNC Symbol;Acc:HGNC...	[more]
KLC4	1.200e-8	25.38	kinesin light chain 4 [Source:HGNC Symbol;Acc:HGNC...	[more]
KLC4	1.200e-8	25.38	kinesin light chain 4 [Source:HGNC Symbol;Acc:HGNC...	[more]
KLC4	1.568e-8	25.38	kinesin light chain 4 [Source:HGNC Symbol;Acc:HGNC...	[more]
KLC2	2.374e-8	26.44	kinesin light chain 2 [Source:HGNC Symbol;Acc:HGNC...	[more]

BLAST of SMED30019363 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
klc-2	7.895e-8	31.90	Kinesin light chain [Source:UniProtKB/Swiss-Prot;...	[more]
klc-2	7.895e-8	31.90	Kinesin light chain [Source:UniProtKB/Swiss-Prot;...	[more]
klc-2	8.696e-8	31.90	Kinesin light chain [Source:UniProtKB/Swiss-Prot;...	[more]
klc-2	8.696e-8	31.90	Kinesin light chain [Source:UniProtKB/Swiss-Prot;...	[more]
klc-2	1.144e-7	31.90	Kinesin light chain [Source:UniProtKB/Swiss-Prot;...	[more]

BLAST of SMED30019363 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Klc	2.242e-8	27.27	gene:FBgn0010235 transcript:FBtr0331556	[more]
Klc	2.366e-8	27.27	gene:FBgn0010235 transcript:FBtr0075951	[more]
Klc	2.366e-8	27.27	gene:FBgn0010235 transcript:FBtr0331555	[more]

BLAST of SMED30019363 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
klc1a	1.259e-7	35.48	kinesin light chain 1a [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
klc1a	1.403e-7	35.48	kinesin light chain 1a [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
klc3	1.060e-6	31.90	kinesin light chain 3 [Source:ZFIN;Acc:ZDB-GENE-06...	[more]
klc4	2.861e-6	34.04	kinesin light chain 4 [Source:ZFIN;Acc:ZDB-GENE-05...	[more]
klc4	2.861e-6	34.04	kinesin light chain 4 [Source:ZFIN;Acc:ZDB-GENE-05...	[more]

BLAST of SMED30019363 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hira	9.752e-8	35.11	histone cell cycle regulator [Source:Xenbase;Acc:X...	[more]
hira	9.765e-8	35.11	histone cell cycle regulator [Source:Xenbase;Acc:X...	[more]
hira	9.841e-8	35.11	histone cell cycle regulator [Source:Xenbase;Acc:X...	[more]
hira	1.052e-7	35.11	histone cell cycle regulator [Source:Xenbase;Acc:X...	[more]
hira	1.068e-7	35.11	histone cell cycle regulator [Source:Xenbase;Acc:X...	[more]

BLAST of SMED30019363 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Klc2	1.390e-8	26.44	kinesin light chain 2 [Source:MGI Symbol;Acc:MGI:1...	[more]
Klc2	1.400e-8	26.44	kinesin light chain 2 [Source:MGI Symbol;Acc:MGI:1...	[more]
Klc2	1.400e-8	26.44	kinesin light chain 2 [Source:MGI Symbol;Acc:MGI:1...	[more]
Klc2	1.400e-8	26.44	kinesin light chain 2 [Source:MGI Symbol;Acc:MGI:1...	[more]
Klc1	5.110e-8	32.76	kinesin light chain 1 [Source:MGI Symbol;Acc:MGI:1...	[more]

BLAST of SMED30019363 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O88448\|KLC2_MOUSE	7.648e-9	24.40	Kinesin light chain 2 OS=Mus musculus OX=10090 GN=...	[more]
sp\|Q9NSK0\|KLC4_HUMAN	5.761e-8	25.38	Kinesin light chain 4 OS=Homo sapiens OX=9606 GN=K...	[more]
sp\|Q2HJJ0\|KLC4_BOVIN	5.846e-8	25.31	Kinesin light chain 4 OS=Bos taurus OX=9913 GN=KLC...	[more]
sp\|Q9H0B6\|KLC2_HUMAN	1.140e-7	26.44	Kinesin light chain 2 OS=Homo sapiens OX=9606 GN=K...	[more]
sp\|P46824\|KLC_DROME	2.372e-7	27.27	Kinesin light chain OS=Drosophila melanogaster OX=...	[more]

BLAST of SMED30019363 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A498MX94	1.378e-9	26.72	Kinesin light chain 2 OS=Labeo rohita OX=84645 GN=...	[more]
A0A498MI54	1.382e-9	26.72	Kinesin light chain 2 OS=Labeo rohita OX=84645 GN=...	[more]
A0A067QTL0	3.637e-9	24.62	Kinesin light chain (Fragment) OS=Zootermopsis nev...	[more]
A0A553Q0D6	3.987e-9	25.16	TPR_REGION domain-containing protein (Fragment) OS...	[more]
A0A553Q0A0	3.993e-9	25.16	TPR_REGION domain-containing protein (Fragment) OS...	[more]

BLAST of SMED30019363 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000055945.1	8.515e-10	26.46	kinesin light chain 2-like [Source:NCBI gene;Acc:1...	[more]
ENSAMXT00000055398.1	8.515e-10	26.46	kinesin light chain 2-like [Source:NCBI gene;Acc:1...	[more]
ENSAMXT00000032282.1	8.515e-10	26.46	kinesin light chain 2-like [Source:NCBI gene;Acc:1...	[more]
ENSAMXT00000031936.1	8.515e-10	26.46	kinesin light chain 2-like [Source:NCBI gene;Acc:1...	[more]
ENSAMXT00000044075.1	8.818e-10	26.46	kinesin light chain 2-like [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30019363 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000009139.1	6.253e-10	32.61	pep scaffold:Pmarinus_7.0:GL480151:1445:19017:1 ge...	[more]
klc1a	6.316e-10	24.19	kinesin light chain 1a [Source:ZFIN;Acc:ZDB-GENE-0...	[more]

BLAST of SMED30019363 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30019363 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO43009	8.552e-8	31.90	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30019363 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000016251.2	7.216e-9	25.10	kinesin light chain 1 [Source:NCBI gene;Acc:101171...	[more]
klc1a	5.967e-8	36.56	kinesin light chain 1 [Source:NCBI gene;Acc:101163...	[more]
klc1a	6.350e-8	36.17	kinesin light chain 1 [Source:NCBI gene;Acc:101163...	[more]
ENSORLT00000010217.2	1.315e-7	24.50	kinesin light chain 2 [Source:NCBI gene;Acc:101159...	[more]
ENSORLT00000010213.2	1.967e-7	23.96	kinesin light chain 2 [Source:NCBI gene;Acc:101159...	[more]

BLAST of SMED30019363 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000076903.1	0.000e+0	99.55	SMESG000076903.1	[more]
SMESG000010480.1	1.397e-25	24.17	SMESG000010480.1	[more]
SMESG000043408.1	1.785e-7	23.42	SMESG000043408.1	[more]
SMESG000007554.1	3.309e-6	29.21	SMESG000007554.1	[more]
SMESG000042588.1	3.457e-6	22.29	SMESG000042588.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30019363 ID=SMED30019363|Name=SMED30019363|organism=Schmidtea mediterranea sexual|type=transcript|length=1407bp
AATCAATAATGATAATAATTATTACATAAAATGGCTTTAGTAAGAGGTTT
TGAAGGAAATAACATGTTACCACTTCTATTATATTTGGAAAGATTTTTCA
ACCAATATTCCGAATATTTTGTAAATGAAAAGAATCGAGTTACCCAAAAT
AAACCAAGAGATTCACTGGGCAGCCGTATACATTTATTAAGTTTAAATTA
CGTCCAAACCATTTCTGACAAAATAGATTTCATCAAGTATGACTATGAAA
TCATTCAAGAATGTACAAAATTGTTGCGTTATATCGACAGTGAAATGGCT
TTTATGACAATGGAAGTTAAAAGGATACTTAATGAAGAGACCGTAATTGA
CAATGAAATTTTTTCAGTGCAGAAAGAACAGAAAAAACTCATTACAGAAA
TCAACAATATGGAATTGGAAAAGGAATACTGGAATGTTGTATTCAGAATG
AGAAATATCACTTTGTTAGGAGAAGAAAGCAAACAAGAAATGAAACTAAA
CACTGGAGAGGATGATGAATTGGAAGAAAACCCTTCGGATTCAATCAAAC
CGCTTTTGATAATCTGCAACGAATTTATTCAAGATAACCGTTTTGAGATG
GCTGAAGGTCTTGCGACAGATAAACTGAAAGAATTGTTGAAAAACTTGAA
AAATCAACCCAACCTTGATGTAGCATCGGTATATGCGATTTTAGCGAAAA
TACATGCAGTAGAAAATGACTACCCATCAGCTTTGGATAATTTTAAATCG
TGTTTAGAAATCCGAGAAAGACTGCTGGATAAGTTTCATCCAGATATTGG
GACTATCAAATACAATTACGGCTTGACTTTGATAAAAGTAAAAGATTTCA
AAGAAGCTGAAAAGCATTTAACGAGCGCACTGGAAATCAAGCAAAACGAA
TTGGGGAAGGAACACAAAACAATTGCAATGATTTATAACTTTTTGGGGAA
AATAAACGTTTTAACAAACAAACAAATCGTTGCTGTCAACTTTTACAGAC
TGGCGTTGAATATTTTGGACAATTTATCAACAGAATCTTTCGAATTGACA
TATTTGTACATTTCTTTGGCGATTTGTCGGATTTATGTGGAAATGAAAAT
TTCTACGGAAGCCGATAATCATTTGGAGAAATTATTTAATTTAATTGAAA
CCCGAATTGGGTTTGATACTTTCCAGAAAAAATCTTGTTACATTTATTTT
AAAAGGCACCTAAATGATGCAAAAGGTGAACATCTTTTTACGTTTTACGG
GAGCTTCTCTGAATATGATATATTAGATTTGTTTGAAATTCTCGTCAAAC
ATTTCAAAAACAATCCAAAGGTCATCGAAGCCATTGATATCTGGAAATAT
TTATCTATACAAAGTACGTAAAAGTTATAAAAATTTAAATAAAATTCATC
TGTGACT

protein sequence of SMED30019363-orf-1

>SMED30019363-orf-1 ID=SMED30019363-orf-1|Name=SMED30019363-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=447bp
MALVRGFEGNNMLPLLLYLERFFNQYSEYFVNEKNRVTQNKPRDSLGSRI
HLLSLNYVQTISDKIDFIKYDYEIIQECTKLLRYIDSEMAFMTMEVKRIL
NEETVIDNEIFSVQKEQKKLITEINNMELEKEYWNVVFRMRNITLLGEES
KQEMKLNTGEDDELEENPSDSIKPLLIICNEFIQDNRFEMAEGLATDKLK
ELLKNLKNQPNLDVASVYAILAKIHAVENDYPSALDNFKSCLEIRERLLD
KFHPDIGTIKYNYGLTLIKVKDFKEAEKHLTSALEIKQNELGKEHKTIAM
IYNFLGKINVLTNKQIVAVNFYRLALNILDNLSTESFELTYLYISLAICR
IYVEMKISTEADNHLEKLFNLIETRIGFDTFQKKSCYIYFKRHLNDAKGE
HLFTFYGSFSEYDILDLFEILVKHFKNNPKVIEAIDIWKYLSIQST*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000208	testis
PLANA:0000221	spermatocyte
PLANA:0000222	spermatid

Vocabulary: **INTERPRO**
Term	Definition
IPR019734	TPR_repeat
IPR011990	TPR-like_helical_dom_sf
IPR013026	TPR-contain_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0003777	microtubule motor activity

Vocabulary: **cellular component**
Term	Definition
GO:0005871	kinesin complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019734	Tetratricopeptide repeat	SMART	SM00028	tpr_5	coord: 215..248 e-value: 0.95 score: 18.5 coord: 299..332 e-value: 270.0 score: 2.4 coord: 257..290 e-value: 11.0 score: 14.6
IPR019734	Tetratricopeptide repeat	PROSITE	PS50005	TPR	coord: 257..290 score: 8.024
IPR019734	Tetratricopeptide repeat	PROSITE	PS50005	TPR	coord: 215..248 score: 8.201
IPR019734	Tetratricopeptide repeat	PROSITE	PS50005	TPR	coord: 299..332 score: 5.045
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	G3DSA:1.25.40.10		coord: 168..397 e-value: 5.1E-25 score: 90.0
IPR011990	Tetratricopeptide-like helical domain superfamily	SUPERFAMILY	SSF48452	TPR-like	coord: 211..343
None	No IPR available	PFAM	PF13424	TPR_12	coord: 213..288 e-value: 8.8E-11 score: 41.9
None	No IPR available	PANTHER	PTHR45783:SF3	KINESIN LIGHT CHAIN	coord: 183..332
None	No IPR available	PANTHER	PTHR45783	FAMILY NOT NAMED	coord: 183..332
IPR013026	Tetratricopeptide repeat-containing domain	PROSITE	PS50293	TPR_REGION	coord: 215..290 score: 11.282

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SMED30019363

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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