Ast4 protein

Overview
NameAst4 protein (CCW36842.1)
Smed IDSMED30019357
Length (bp)898
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ast4 protein (SMED30019357) t-SNE clustered cells

Violin plots show distribution of expression levels for Ast4 protein (SMED30019357) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ast4 protein (SMED30019357) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ast4 protein (SMED30019357) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019357

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 20

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30019357h1SMcG0003811 SmedASXL_012035SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019357h1SMcG0003811 SmedASXL_012035SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30019357h1SMcG0003811 SmedASXL_012035SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019357h1SMcG0003811 SmedASXL_012035SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003812 Contig34786uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003812 Contig34786newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003811 Contig34786uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003811 Contig34786newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30019357h1SMcG0003810 dd_Smed_v4_76_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30019357h1SMcG0003810 dd_Smed_v4_76_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003812 Contig34786uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003812 Contig34786newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003811 Contig34786uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019357h1SMcG0003811 Contig34786newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30019357h1SMcG0003810 dd_Smed_v4_76_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30019357h1SMcG0003810 dd_Smed_v4_76_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30019357h1SMcG0003811 dd_Smed_v6_76_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019357h1SMcG0003811 dd_Smed_v6_76_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30019357h1SMcG0003811 OX_Smed_1.0.07444ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30019357h1SMcG0003811 OX_Smed_1.0.07444ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ast4 protein vs. Ensembl Human
Match: TLL2 (tolloid like 2 [Source:HGNC Symbol;Acc:HGNC:11844])

HSP 1 Score: 87.0409 bits (214), Expect = 1.992e-18
Identity = 62/194 (31.96%), Postives = 97/194 (50.00%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTT---TYDFDSIMQYPSEAFSCNGK--DTILKK---NGGK--IPFNMEVTKLDAKKVRDYYGC 600
            WP  ++ Y    ++    +   ++ ++ +EK    C+ F+E ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRDQ + I   N+  G   NF   E  ++++   TYDFDSIM Y    FS  G   DTIL +   NG +  I   + +++ D  + R  Y C
Sbjct:  159 WPGGVIPYVIGGNFTGSQRAIFKQAMRHWEKH--TCVTFIE-RTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFS-RGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKC 348
[Close]
BLAST of Ast4 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 85.1149 bits (209), Expect = 4.196e-18
Identity = 63/193 (32.64%), Postives = 92/193 (47.67%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLGG---GCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCNG-KDTIL--KKNGGKIPFNMEVTKL---DAKKVRDYYGC 600
            WP  ++ Y    ++    +   ++ ++ +EK    C+ F+E +SD  + +V      GC + VG   N P +I++   C     V HE  H++GF HEHTRPDRD  + I   N+  G   NF   E  +   L   YDFDSIM Y    FS     DTIL  + + G  P   + T+L   D  + R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIE-RSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast4 protein vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 85.8853 bits (211), Expect = 4.730e-18
Identity = 63/193 (32.64%), Postives = 94/193 (48.70%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCN-GKDTILKK---NGGKIPFNM--EVTKLDAKKVRDYYGC 600
            WP+ ++ +    ++    +   R+ ++ +EK    C+ F+E ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD+ + I   N+  G   NF   E  +   L  TYDFDSIM Y    FS     DTI+ K   NG K P      ++K D  + R  Y C
Sbjct:  130 WPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLE-RTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKC 319
[Close]
BLAST of Ast4 protein vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 85.5001 bits (210), Expect = 5.922e-18
Identity = 63/193 (32.64%), Postives = 94/193 (48.70%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCN-GKDTILKK---NGGKIPFNM--EVTKLDAKKVRDYYGC 600
            WP+ ++ +    ++    +   R+ ++ +EK    C+ F+E ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD+ + I   N+  G   NF   E  +   L  TYDFDSIM Y    FS     DTI+ K   NG K P      ++K D  + R  Y C
Sbjct:  130 WPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLE-RTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKC 319
[Close]
BLAST of Ast4 protein vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 85.5001 bits (210), Expect = 7.369e-18
Identity = 63/193 (32.64%), Postives = 94/193 (48.70%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCN-GKDTILKK---NGGKIPFNM--EVTKLDAKKVRDYYGC 600
            WP+ ++ +    ++    +   R+ ++ +EK    C+ F+E ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD+ + I   N+  G   NF   E  +   L  TYDFDSIM Y    FS     DTI+ K   NG K P      ++K D  + R  Y C
Sbjct:  130 WPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLE-RTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKC 319
[Close]
BLAST of Ast4 protein vs. Ensembl Celegans
Match: nas-14 (Zinc metalloproteinase nas-14 [Source:UniProtKB/Swiss-Prot;Acc:Q19269])

HSP 1 Score: 93.5893 bits (231), Expect = 3.131e-21
Identity = 67/217 (30.88%), Postives = 105/217 (48.39%), Query Frame = -2 
Query:   58 DMVLSSQDVLRINGFKAAQGVHWPNKL-----LRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD----VYATVVLGGGCSANVGY-NKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGC---KLTTTYDFDSIMQYPSEAFSCNGKDTIL-KKNGGKIPFNMEVTKLDAKKVRDYYGC 666
            D +L   ++ R     A   V +P+KL     + Y  +    +  +  I +    Y+ +   C+RFV +  D    +Y    +  GCS+ VG    N  +S+ + D C ++  + HE  H LGF HEH+R DRD F+ I   N+  G+  NF          L   YD++S+M Y   AFS NGK TI+ K N   +    +++++D+KKV   Y C
Sbjct:   98 DPLLDEDEIFRKPFHSALNLVTYPDKLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTK--TCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAGGNQTVSLEV-DKCFSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQC 311
[Close]
BLAST of Ast4 protein vs. Ensembl Celegans
Match: nas-7 (Zinc metalloproteinase nas-7 [Source:UniProtKB/Swiss-Prot;Acc:P55113])

HSP 1 Score: 87.8113 bits (216), Expect = 1.976e-19
Identity = 59/197 (29.95%), Postives = 94/197 (47.72%), Query Frame = -2 
Query:   58 NGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG--GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLT---TTYDFDSIMQYPSEAFSCNGKDTILKK-NGGKIPFNMEVTKLDAKKVRDYYGC 630
            NG   A  + WPN  + Y   P Y    +  + + +K Y ++   C+RFV  ++     + +G   GC + VG      + ++L + C+    + HE  H++GF HEH R DRD F+ I + N+  G  + F   +  K +     YD+ SI+ Y S AFS NG  T+L K     I    + + +D  K+   Y C
Sbjct:   80 NGVSRAAKL-WPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRFVPRQTGEPDYLFIGKVDGCFSEVGRTSGVQV-LSLDNGCMEYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDISKINRMYNC 272
[Close]
BLAST of Ast4 protein vs. Ensembl Celegans
Match: nas-1 (Zinc metalloproteinase nas-1 [Source:UniProtKB/Swiss-Prot;Acc:O62243])

HSP 1 Score: 85.8853 bits (211), Expect = 2.136e-19
Identity = 59/189 (31.22%), Postives = 87/189 (46.03%), Query Frame = -2 
Query:  127 DMVLSSQDVLRINGFK-AAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG--GGCSAN---VGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLT---TTYDFDSIMQYPSEAFSCNGKDTILKKNG 666
            DM +  Q   R  G   AA+   WPN  + Y    +Y    +  +      Y K    C+RFV +       +V+    GC A+   VG  +     ++L D C++   + HE  H++GF+HEH R DRD ++ I + NV  G   +F       L+     YD+ SIM Y +   S NGK+TI  KN 
Sbjct:   57 DMRIPFQRFKRGGGVAVAAEKDKWPNGRVPYILSAAYTSAQRAVLARAFDTYAKR--TCIRFVPKSPADKDYIVIQKLDGCYADFSRVGGRQQ----VSLADECIDYATIIHELMHVIGFIHEHQREDRDSYVSILYQNVIQGANTDFDKLSNLGLSYYGEHYDYSSIMHYEANEGSRNGKNTIEAKNS 239
[Close]
BLAST of Ast4 protein vs. Ensembl Celegans
Match: nas-12 (Zinc metalloproteinase nas-12 [Source:UniProtKB/Swiss-Prot;Acc:Q9XTD6])

HSP 1 Score: 84.7297 bits (208), Expect = 1.911e-18
Identity = 68/227 (29.96%), Postives = 103/227 (45.37%), Query Frame = -2 
Query:   76 GDMVLSSQDVLRINGFK----AAQGV--------HWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKS-DVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTT-------YDFDSIMQYPSEAFSCNGKDTILK---------KNGGKIPFNMEVTKLDAKKV 669
            GDM+L+   ++R    K    + +GV         W N ++ Y   P Y    K  +   L+ +E+   +C +FVE  + + Y  +V   GC + VG       +++L   C+    + HE  H +GF HEH RPDRD F+++++ NV  G   NF      KL T+       YDF SIM Y   AF     DT  +         K G  +  NM+ T  D +K+
Sbjct:   48 GDMLLTPAQLIRYENSKDSDLSIRGVSIKGSSMNRWSNNIVPYVISPQYSPAQKQILVSSLRYFERV--SCFKFVERTTQNDYLFIVPLDGCYSYVG-KIGGRQTLSLAADCIADYIIWHEMMHAIGFEHEHQRPDRDSFIRVDYANVIPGQMINFD-----KLKTSHVEYPDIYDFKSIMHYDGYAF--GRVDTARRVRLATMTPLKPGVTLEDNMKFTATDIEKL 264
[Close]
BLAST of Ast4 protein vs. Ensembl Celegans
Match: nas-15 (Zinc metalloproteinase nas-15 [Source:UniProtKB/Swiss-Prot;Acc:P55115])

HSP 1 Score: 85.5001 bits (210), Expect = 2.289e-18
Identity = 62/187 (33.16%), Postives = 95/187 (50.80%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFV-EEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFY-LCEGC--KLTTTYDFDSIMQYPSEAFSCNGKDTIL-KKNGGKIPFNMEVTKLDAKKVRDYYGC 600
            WP   + YT    Y   +++ I   ++ Y      C+R+V +E +DV Y  +    GC + VG       S++L   C+ +  + HE  H +GF HE +R DRD  + I + N+ +G+   F     G    L T YD+ SIM Y ++AFS NG+ T++ KKNG  I      +K+D  K+   YGC
Sbjct:  123 WPEGRIPYTISSQYSSYSRSLIAASMQEYASH--TCIRWVPKEAADVNYVHIYPDRGCYSMVGKMGGK-QSLSLGSGCIQKGIILHELMHAVGFFHEQSRTDRDDHITIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGATIGQRNGFSKVDKFKINTLYGC 306
[Close]
BLAST of Ast4 protein vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 94.7449 bits (234), Expect = 8.791e-23
Identity = 67/246 (27.24%), Postives = 117/246 (47.56%), Query Frame = -2 
Query:   64 ISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRING------FKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVL---GGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTT---TYDFDSIMQYPSEAFSCNGKDTILK-KNGGKIPFNMEVTKLDAKKVRDYY 762
            + ++ L+V+  L S+  G  +  IK+      D++L S+ +    G       K+    +W  + L Y++   +   +   I   +   E     C++F   +      VV+   G GC + VGY      ++ L   C++ R ++HE  H LGF H H+ P RD++++I+  N+ SG  +NF       +T     YD+DSIM Y   AFS NG+ TI+  K+  KI    +++  D + ++  Y
Sbjct:    1 MHYLALAVIFGLGSSANG--RPSIKLQE---DDIILISEQLQYFEGNLEGRVVKSWSEYYWKGRTLVYSYAGGFSSLDIASIESAMA--EISSKTCVKFRRTEYKREPQVVIQKEGSGCWSYVGYLGRADQTLNLGSGCMSNRTIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAFSKNGQSTIVPLKSHAKIGQATQMSPKDVQTLKRMY 239
[Close]
BLAST of Ast4 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 92.8189 bits (229), Expect = 2.879e-21
Identity = 65/219 (29.68%), Postives = 105/219 (47.95%), Query Frame = -2 
Query:   58 KISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG--GGCSANVGYNKNYPLSITLPDP-CVNRRNVK-HEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK-LTTTYDFDSIMQYPSEAFSCNGKDTIL--KKNGG-KIPFNMEVTKLDAKKVRDYYGC 690
            ++ ++  GDM++   D++  NG    Q   WPN ++ Y    ++  ++   I   +  Y +    C+RFV+  S+     + G   GC ++VG        + L  P C++R     HE  H LGF+HE  R +RD ++ I++ NV S   NNF      +     YD+ S+M Y   AFS NG+ TIL  + NG  K+      +  D +K+   Y C
Sbjct:   88 ELGSYLEGDMLVPQTDLIMKNGLPT-QSSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVG-RVGGKQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302
[Close]
BLAST of Ast4 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 92.8189 bits (229), Expect = 2.879e-21
Identity = 65/219 (29.68%), Postives = 105/219 (47.95%), Query Frame = -2 
Query:   58 KISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG--GGCSANVGYNKNYPLSITLPDP-CVNRRNVK-HEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK-LTTTYDFDSIMQYPSEAFSCNGKDTIL--KKNGG-KIPFNMEVTKLDAKKVRDYYGC 690
            ++ ++  GDM++   D++  NG    Q   WPN ++ Y    ++  ++   I   +  Y +    C+RFV+  S+     + G   GC ++VG        + L  P C++R     HE  H LGF+HE  R +RD ++ I++ NV S   NNF      +     YD+ S+M Y   AFS NG+ TIL  + NG  K+      +  D +K+   Y C
Sbjct:   88 ELGSYLEGDMLVPQTDLIMKNGLPT-QSSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVG-RVGGKQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302
[Close]
BLAST of Ast4 protein vs. Ensembl Fly
Match: CG15253 (gene:FBgn0028948 transcript:FBtr0080782)

HSP 1 Score: 85.5001 bits (210), Expect = 3.074e-19
Identity = 69/219 (31.51%), Postives = 108/219 (49.32%), Query Frame = -2 
Query:   58 GDMVLS--SQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD--VYATVVLG-GGCSANVGY-NKNYPLSIT---LPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNF--YLCEGCK-LTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGK---IPFNMEVTKLDAKKVRDYYGC 669
            GDMV S  S+++ R   ++      WPN+++ Y  +    ++++N I     I + E  +CL F E  +D   Y  V    GGC + +GY N+   L++    +   C     + HEF H LGF H+ +  DRD +++I   N+  G+  NF  Y  E        YD+ S+M Y   AFS NG+ TIL    GK   I   +E+++ D +K+   Y C
Sbjct:   38 GDMVPSGSSRNIWRNETYR------WPNRIIYYHINSYIDEEHRNHI--VSAIQKIESISCLTFKEATTDQKYYVNVTSEEGGCFSYIGYLNRVQQLNLQNNEIGVGCFRLYTIVHEFLHALGFFHQQSAADRDDYVQIVEENITEGMEFNFDKYTEETVNDFGEKYDYGSVMHYGPYAFSKNGERTILALEEGKEDVIGQRLELSETDIRKLNAIYKC 248
[Close]
BLAST of Ast4 protein vs. Ensembl Fly
Match: CG15254 (gene:FBgn0028949 transcript:FBtr0343718)

HSP 1 Score: 82.4185 bits (202), Expect = 3.740e-18
Identity = 72/251 (28.69%), Postives = 119/251 (47.41%), Query Frame = -2 
Query:   58 LSVVIELCSAQCGC---TQKGIKI-----SNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD--VYATVVL-GGGCSANVGY-NKNYPLSI---TLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNF--YLCEGCK-LTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPF---NMEVTKLDAKKVRDYYGC 747
            L++V+   ++ C     TQ  I+      + +  GDMV S +     NG +  +   WPN+++ Y  +     +++N I   ++I E+   +CL F E  +D   Y  V    GGC + VGY N+   L++    L   C     + HEF H LGF H+ +  +RD +++I   N+  G   NF  Y  E  +     YD+ S++ Y + AFS NG+ TI+    G        +++T+ D  K+   Y C
Sbjct:    6 LTLVVIFLASSCSAAPTTQNRIETDPELTAGYIEGDMVPSPEGR---NGLRN-ETFRWPNRIVYYYINRDIDTEHRNHILRGIRIIEQ--SSCLVFKEATTDQEYYVNVTSEAGGCYSYVGYRNRVQQLNLQTYALDTGCFRLGTIVHEFLHALGFYHQQSTWNRDDYVRIAEENITEGTEGNFNKYDNETVEDYGEPYDYSSVLHYTAYAFSKNGEMTIVPLQEGAEELMGQRLQMTQSDINKLNVMYKC 250
[Close]
BLAST of Ast4 protein vs. Ensembl Zebrafish
Match: CU326366.2 (pep chromosome:GRCz11:10:42887204:42898220:-1 gene:ENSDARG00000068593.5 transcript:ENSDART00000099270.5 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU326366.2)

HSP 1 Score: 85.1149 bits (209), Expect = 8.920e-19
Identity = 63/188 (33.51%), Postives = 93/188 (49.47%), Query Frame = -2 
Query:   58 WPNK----LLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVG-YNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTIL-KKNGGKIPFNMEVTKLDAKKVRDYYGC 600
            WP K     + Y+      DK  + I   LK+  K+   C++F    ++  Y        C++ VG      P+   L  P  N  N+ HE  H LG  HEH+RPDRD+++ I + N+  G  +NF + +G  L   YD DSI+ Y  + FS NG  TI+ KK G KI     ++ LD +++R  Y C
Sbjct:   27 WPEKDGEVSVPYSIASGLEDKTGH-ILAALKMVSKK--TCVKFHHHTTEEDYLHFKPDRMCASLVGCAGGEQPI---LVGPKCNAGNICHEILHSLGLYHEHSRPDRDKYITILYDNIMPGKESNFKVKKGNTLGLEYDLDSILHYGDDCFSRNGNHTIIPKKKGVKIGQRTHMSVLDVERLRRLYHC 208
[Close]
BLAST of Ast4 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 83.1889 bits (204), Expect = 3.925e-18
Identity = 53/183 (28.96%), Postives = 87/183 (47.54%), Query Frame = -2 
Query:   58 NKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVGYNK-NYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEV--TKLDAKKVRDYYGC 594
            N  + Y+   +Y D +   I+E +++ E++   C+RFV       Y  +    GC + +G       +S+  PD C       HE  H LGF+HE +R DRD+++ I + N+      NF   +   L T YD+ S+M +   AFS +G+ TI+ K    +     +  + LD  K+   Y C
Sbjct:   94 NVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQPKTGCWSYLGARGGRQTISLQSPD-CTGSGVTVHELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEPTIVPKRNWNVKIGQRLGPSDLDIMKINKLYSC 273
[Close]
BLAST of Ast4 protein vs. Ensembl Zebrafish
Match: bmp1a (bone morphogenetic protein 1a [Source:ZFIN;Acc:ZDB-GENE-060818-1])

HSP 1 Score: 85.5001 bits (210), Expect = 4.064e-18
Identity = 61/192 (31.77%), Postives = 89/192 (46.35%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVL--GGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCN-GKDTILKK---NGGKIPFNM--EVTKLDAKKVRDYYGC 600
            WP  ++ Y    ++    +   R+ ++ +EK    C+ F+E  ++    V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD+ + I   N+  G   NF   E  +   L   YDFDSIM Y    FS     DTIL +   NG + P      ++K D  + R  Y C
Sbjct:  119 WPEGVIPYVISGNFSGSQRAIFRQAMRHWEKH--TCVTFIERTTEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDEHVSIIRDNIQPGQEYNFLKMEPGEVDSLGEVYDFDSIMHYARNTFSRGIFLDTILPRYDVNGVRPPIGQRTRLSKGDIAQARKLYKC 308
[Close]
BLAST of Ast4 protein vs. Ensembl Zebrafish
Match: bmp1a (bone morphogenetic protein 1a [Source:ZFIN;Acc:ZDB-GENE-060818-1])

HSP 1 Score: 85.5001 bits (210), Expect = 4.076e-18
Identity = 61/192 (31.77%), Postives = 89/192 (46.35%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVL--GGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCN-GKDTILKK---NGGKIPFNM--EVTKLDAKKVRDYYGC 600
            WP  ++ Y    ++    +   R+ ++ +EK    C+ F+E  ++    V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD+ + I   N+  G   NF   E  +   L   YDFDSIM Y    FS     DTIL +   NG + P      ++K D  + R  Y C
Sbjct:  119 WPEGVIPYVISGNFSGSQRAIFRQAMRHWEKH--TCVTFIERTTEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDEHVSIIRDNIQPGQEYNFLKMEPGEVDSLGEVYDFDSIMHYARNTFSRGIFLDTILPRYDVNGVRPPIGQRTRLSKGDIAQARKLYKC 308
[Close]
BLAST of Ast4 protein vs. Ensembl Zebrafish
Match: tll1 (tolloid-like 1 [Source:NCBI gene;Acc:474335])

HSP 1 Score: 83.5741 bits (205), Expect = 2.006e-17
Identity = 63/194 (32.47%), Postives = 94/194 (48.45%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGC---KLTTTYDFDSIMQYPSEAFSCNGK--DTIL---KKNGGK--IPFNMEVTKLDAKKVRDYYGC 600
            WP  ++ Y    ++    +  +++ ++ +EK+   C+ F+E K+D  + +V      GC + VG   N P +I++   C     V HE  H++GF HEHTRPDRD  + I   N+  G   NF   E      L   YDFDSIM Y    FS  G   DTIL    +NG +  I     ++K D  + +  Y C
Sbjct:  166 WPGGVIPYVIGGNFTGSQRAMLKQAMRHWEKQ--TCVTFIE-KTDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRDNIQPGQEYNFIKMEPGDVNSLGEPYDFDSIMHYARNTFS-RGMFLDTILPSRDENGVRPAIGQRTRLSKGDISQAKKLYRC 355
[Close]
BLAST of Ast4 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 94.3597 bits (233), Expect = 4.411e-21
Identity = 58/178 (32.58%), Postives = 83/178 (46.63%), Query Frame = -2 
Query:   58 YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVE-EKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFS-CNGKDTILKKNGGKIPFNM--EVTKLDAKKVRDYYGC 579
            YT D  Y +   N I   +++Y      C++FV     D Y  +  G GC + +G      +       C +     HE  H LGF+HEH+R DRD ++ I +  +  G   NF   E   L TTYD+ SIM YP+ AFS   G++TI+ K    +       +T LD  K+   Y C
Sbjct:   85 YTLDEQYSNNEVNTIMTAMQVYATL--TCVQFVPYTDEDDYIAITSGDGCWSYMGRQGGAQVVSVQKTYCTSEGTTMHELNHALGFVHEHSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 260
[Close]
BLAST of Ast4 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 93.9745 bits (232), Expect = 6.067e-21
Identity = 58/178 (32.58%), Postives = 83/178 (46.63%), Query Frame = -2 
Query:   58 YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVE-EKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFS-CNGKDTILKKNGGKIPFNM--EVTKLDAKKVRDYYGC 579
            YT D  Y +   N I   +++Y      C++FV     D Y  +  G GC + +G      +       C +     HE  H LGF+HEH+R DRD ++ I +  +  G   NF   E   L TTYD+ SIM YP+ AFS   G++TI+ K    +       +T LD  K+   Y C
Sbjct:  125 YTLDEQYSNNEVNTIMTAMQVYATL--TCVQFVPYTDEDDYIAITSGDGCWSYMGRQGGAQVVSVQKTYCTSEGTTMHELNHALGFVHEHSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 300
[Close]
BLAST of Ast4 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 91.6633 bits (226), Expect = 3.896e-20
Identity = 58/178 (32.58%), Postives = 83/178 (46.63%), Query Frame = -2 
Query:   58 YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVE-EKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFS-CNGKDTILKKNGGKIPFNM--EVTKLDAKKVRDYYGC 579
            YT D  Y     N I   +++Y      C++FV     + Y  +  G GC + +G      +       C +     HE  H LGF+HEH+R DRD ++ I +  + SG   NF   E   L TTYD+ SIM YP+ AFS   G++TI+ K    +       +T LD  K+   Y C
Sbjct:   90 YTLDKQYSSDQINTITSAMEVYSTL--TCVQFVPYTDEEDYIAITSGDGCWSFMGRQGGAQVVSVEKGYCTSEGTTMHELNHALGFVHEHSRSDRDNYVNIMYQYISSGDDVNFEKMETNNLDTTYDYRSIMHYPAWAFSNTTGQNTIVAKPNPNVTIGAGNTMTSLDIIKINRLYEC 265
[Close]
BLAST of Ast4 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 90.8929 bits (224), Expect = 7.428e-20
Identity = 57/178 (32.02%), Postives = 81/178 (45.51%), Query Frame = -2 
Query:   58 YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVE-EKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFS-CNGKDTILKKNGGKIPF--NMEVTKLDAKKVRDYYGC 579
            YT D  Y     N I   +++Y      C++FV     + Y  +  G GC + +G      +       C +     HE  H LGF+HE +R DRD ++ I +  +  G   NF   E   L TTYD+ SIM YP+ AFS   G++TI+ K     P      +T LD  K+   Y C
Sbjct:   90 YTLDKQYSSDQINTITSAMEVYSTL--TCVQFVPYTDEEDYIAITSGDGCWSYMGRQGGAQVVSVEKGYCTSEGTTMHELNHALGFVHEQSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKLNPNTPLGPGSTMTNLDITKINRLYQC 265
[Close]
BLAST of Ast4 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 90.5077 bits (223), Expect = 8.645e-20
Identity = 57/178 (32.02%), Postives = 81/178 (45.51%), Query Frame = -2 
Query:   58 YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVE-EKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFS-CNGKDTILKKNGGKIPF--NMEVTKLDAKKVRDYYGC 579
            YT D  Y     N I   +++Y      C++FV     + Y  +  G GC + +G      +       C +     HE  H LGF+HE +R DRD ++ I +  +  G   NF   E   L TTYD+ SIM YP+ AFS   G++TI+ K     P      +T LD  K+   Y C
Sbjct:   90 YTLDKQYSSDQINTITSAMEVYSTL--TCVQFVPYTDEEDYIAITSGDGCWSYMGRQGGAQVVSVEKGYCTSEGTTMHELNHALGFVHEQSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKLNPNTPLGPGSTMTNLDITKINRLYQC 265
[Close]
BLAST of Ast4 protein vs. Ensembl Mouse
Match: Tll2 (tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044])

HSP 1 Score: 87.4261 bits (215), Expect = 1.210e-18
Identity = 63/194 (32.47%), Postives = 97/194 (50.00%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTT---TYDFDSIMQYPSEAFSCNGK--DTILKK---NGGK--IPFNMEVTKLDAKKVRDYYGC 600
            WP  ++ Y    ++    +   ++ ++ +EK    C+ FVE ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRDQ + I   N+  G   NF   E  ++++   TYDFDSIM Y    FS  G   DTIL +   NG +  I   + +++ D  + R  Y C
Sbjct:  156 WPGGVIPYVIGGNFTGTQRAIFKQAMRHWEKH--TCVTFVE-RTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFS-RGVFLDTILPRRDDNGVRPTIGQRVRLSQGDIAQARKLYKC 345
[Close]
BLAST of Ast4 protein vs. Ensembl Mouse
Match: Tll2 (tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044])

HSP 1 Score: 85.1149 bits (209), Expect = 7.432e-18
Identity = 51/152 (33.55%), Postives = 78/152 (51.32%), Query Frame = -2 
Query:  163 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTT---TYDFDSIMQYPSEAFS 600
            WP  ++ Y    ++    +   ++ ++ +EK    C+ FVE ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRDQ + I   N+  G   NF   E  ++++   TYDFDSIM Y    FS
Sbjct:  156 WPGGVIPYVIGGNFTGTQRAIFKQAMRHWEKH--TCVTFVE-RTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFS 304
[Close]
BLAST of Ast4 protein vs. Ensembl Mouse
Match: Tll1 (tolloid-like [Source:MGI Symbol;Acc:MGI:106923])

HSP 1 Score: 83.1889 bits (204), Expect = 2.882e-17
Identity = 64/194 (32.99%), Postives = 92/194 (47.42%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCNGK--DTIL--KKNGGKIPFNMEVTKL---DAKKVRDYYGC 600
            WP  ++ Y    ++    +   ++ ++ +EK    C+ F E +SD  + +V      GC + VG   N P +I++   C     V HE  H++GF HEHTRPDRD  + I   N+  G   NF   E  +   L   YDFDSIM Y    FS  G   DTIL  + + G  P   + T+L   D  + R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFTE-RSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFS-RGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast4 protein vs. Ensembl Mouse
Match: Bmp1 (bone morphogenetic protein 1 [Source:MGI Symbol;Acc:MGI:88176])

HSP 1 Score: 83.1889 bits (204), Expect = 3.204e-17
Identity = 63/193 (32.64%), Postives = 94/193 (48.70%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFY---LCEGCKLTTTYDFDSIMQYPSEAFSCN-GKDTILKK---NGGK--IPFNMEVTKLDAKKVRDYYGC 600
            WP+ ++ +    ++    +   R+ ++ +EK    C+ F+E ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD+ + I   N+  G   NF    + E   L  TYDFDSIM Y    FS     DTI+ K   NG K  I     ++K D  + R  Y C
Sbjct:  135 WPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLE-RTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEVQEVESLGETYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPSIGQRTRLSKGDIAQARKLYKC 324
[Close]
BLAST of Ast4 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 75.0998 bits (183), Expect = 8.767e-15
Identity = 47/179 (26.26%), Postives = 86/179 (48.04%), Query Frame = -2 
Query:  139 DVLRINGFK--AAQGVHWPNKL-----LRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCV--NRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTIL 645
            D++R + F+  +     WP  +     + +     Y + ++  I +    +E+    C+RFV       + +++   GC + VG +    + ++L   C+   R  V HE  H+LGF HEH+R DRD+++++ +  +  G   NF       +   YD+ S+M Y   AFS  G+ TI+
Sbjct:   77 DIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGGMQV-VSLAPTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTII 252
[Close]
BLAST of Ast4 protein vs. UniProt/SwissProt
Match: sp|Q19269|NAS14_CAEEL (Zinc metalloproteinase nas-14 OS=Caenorhabditis elegans OX=6239 GN=nas-14 PE=2 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 3.983e-20
Identity = 67/217 (30.88%), Postives = 105/217 (48.39%), Query Frame = -2 
Query:   58 DMVLSSQDVLRINGFKAAQGVHWPNKL-----LRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD----VYATVVLGGGCSANVGY-NKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGC---KLTTTYDFDSIMQYPSEAFSCNGKDTIL-KKNGGKIPFNMEVTKLDAKKVRDYYGC 666
            D +L   ++ R     A   V +P+KL     + Y  +    +  +  I +    Y+ +   C+RFV +  D    +Y    +  GCS+ VG    N  +S+ + D C ++  + HE  H LGF HEH+R DRD F+ I   N+  G+  NF          L   YD++S+M Y   AFS NGK TI+ K N   +    +++++D+KKV   Y C
Sbjct:   98 DPLLDEDEIFRKPFHSALNLVTYPDKLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTK--TCVRFVPKTDDDFDYIYVKRNVAFGCSSYVGRAGGNQTVSLEV-DKCFSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLSEMDSKKVNKLYQC 311
[Close]
BLAST of Ast4 protein vs. UniProt/SwissProt
Match: sp|P31579|LCE_ORYLA (Low choriolytic enzyme OS=Oryzias latipes OX=8090 GN=lce PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.670e-18
Identity = 62/213 (29.11%), Postives = 102/213 (47.89%), Query Frame = -2 
Query:   58 GDMVL-SSQDVLRINGFKAAQGVHWP---NKLLR--YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD-VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 669
            GD+VL  +++ ++   F A     WP   N +++  Y    +Y    K  IR  +K + ++   C+ FV   ++  Y ++    GC + +GY  +  + +     C+    ++HE  H LGF HEHTR DRDQ +KI + N+     +NF   +   L T YD+ SIM Y   AF  + K+TI  +      I     ++ +D  +V   Y C
Sbjct:   63 GDLVLPKTRNAMKC--FGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQRFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast4 protein vs. UniProt/SwissProt
Match: sp|P55113|NAS7_CAEEL (Zinc metalloproteinase nas-7 OS=Caenorhabditis elegans OX=6239 GN=nas-7 PE=2 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 2.514e-18
Identity = 59/197 (29.95%), Postives = 94/197 (47.72%), Query Frame = -2 
Query:   58 NGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG--GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLT---TTYDFDSIMQYPSEAFSCNGKDTILKK-NGGKIPFNMEVTKLDAKKVRDYYGC 630
            NG   A  + WPN  + Y   P Y    +  + + +K Y ++   C+RFV  ++     + +G   GC + VG      + ++L + C+    + HE  H++GF HEH R DRD F+ I + N+  G  + F   +  K +     YD+ SI+ Y S AFS NG  T+L K     I    + + +D  K+   Y C
Sbjct:   80 NGVSRAAKL-WPNARIPYAISPHYSPHERALLAKAVKQYHEK--TCIRFVPRQTGEPDYLFIGKVDGCFSEVGRTSGVQV-LSLDNGCMEYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDISKINRMYNC 272
[Close]
BLAST of Ast4 protein vs. UniProt/SwissProt
Match: sp|O62243|NAS1_CAEEL (Zinc metalloproteinase nas-1 OS=Caenorhabditis elegans OX=6239 GN=nas-1 PE=3 SV=2)

HSP 1 Score: 85.8853 bits (211), Expect = 2.717e-18
Identity = 59/189 (31.22%), Postives = 87/189 (46.03%), Query Frame = -2 
Query:  127 DMVLSSQDVLRINGFK-AAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG--GGCSAN---VGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLT---TTYDFDSIMQYPSEAFSCNGKDTILKKNG 666
            DM +  Q   R  G   AA+   WPN  + Y    +Y    +  +      Y K    C+RFV +       +V+    GC A+   VG  +     ++L D C++   + HE  H++GF+HEH R DRD ++ I + NV  G   +F       L+     YD+ SIM Y +   S NGK+TI  KN 
Sbjct:   57 DMRIPFQRFKRGGGVAVAAEKDKWPNGRVPYILSAAYTSAQRAVLARAFDTYAKR--TCIRFVPKSPADKDYIVIQKLDGCYADFSRVGGRQQ----VSLADECIDYATIIHELMHVIGFIHEHQREDRDSYVSILYQNVIQGANTDFDKLSNLGLSYYGEHYDYSSIMHYEANEGSRNGKNTIEAKNS 239
[Close]
BLAST of Ast4 protein vs. UniProt/SwissProt
Match: sp|Q9WVM6|TLL2_MOUSE (Tolloid-like protein 2 OS=Mus musculus OX=10090 GN=Tll2 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.466e-18
Identity = 63/194 (32.47%), Postives = 97/194 (50.00%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTT---TYDFDSIMQYPSEAFSCNGK--DTILKK---NGGK--IPFNMEVTKLDAKKVRDYYGC 600
            WP  ++ Y    ++    +   ++ ++ +EK    C+ FVE ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRDQ + I   N+  G   NF   E  ++++   TYDFDSIM Y    FS  G   DTIL +   NG +  I   + +++ D  + R  Y C
Sbjct:  156 WPGGVIPYVIGGNFTGTQRAIFKQAMRHWEKH--TCVTFVE-RTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFS-RGVFLDTILPRRDDNGVRPTIGQRVRLSQGDIAQARKLYKC 345
[Close]
BLAST of Ast4 protein vs. TrEMBL
Match: A0A060Q6Y6 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast4 PE=2 SV=1)

HSP 1 Score: 496.893 bits (1278), Expect = 1.099e-176
Identity = 238/241 (98.76%), Postives = 239/241 (99.17%), Query Frame = -2 
Query:   58 MLLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 780
            M LKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKN IREFLKIYEKELGNCLRF+EEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC
Sbjct:    1 MTLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNAIREFLKIYEKELGNCLRFLEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 241
[Close]
BLAST of Ast4 protein vs. TrEMBL
Match: A0A060Q715 (Metalloendopeptidase (Fragment) OS=Schmidtea mediterranea OX=79327 GN=Ast7 PE=2 SV=1)

HSP 1 Score: 382.104 bits (980), Expect = 2.357e-131
Identity = 179/236 (75.85%), Postives = 200/236 (84.75%), Query Frame = -2 
Query:   58 IISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 765
            +I F+ L  +  LC AQCGCTQKGIKI++FNVGDMVLS  ++L+ING+KAA G  WP   LRYTFDPS+PDKNKNP+R+FLK YEKELGNC++F EEKS V+ATVV GGGCSANVGYN  YPL +TL DPCVN+RNVKHEFTH LGF HEHTRPDRD  L I+F NV   LC NFYLCEGCK TT YDFDSIMQYPSEAF+CNGK+TIL+KNGGKI FNMEVTKLDAKKVRDYYGC
Sbjct:    1 VICFL-LITLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 235
[Close]
BLAST of Ast4 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 5.950e-49
Identity = 91/248 (36.69%), Postives = 140/248 (56.45%), Query Frame = -2 
Query:   58 LKTIISFITLSVVIELCSAQCGC-TQKGIK--ISNFNVGDMVLSSQDVLRINGFKA--AQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATV-VLGGGCSANVGYN--KNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSC-NGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 774
            ++++   + +   +   S QCGC  QK +K  + +  +GDMVL   D+  INGFKA  +    WPN  + ++F P++P   K  +RE L   +K+LGNC++F E  +  Y  V  L  GC + +GY    N PL++  P    ++  VKHEF H LGFMH H R DRD  + I++  + +  C+ F  CEGC L   Y+ +S+M YPS  F+C  G++ + K++GG I +N   ++ D   VR +Y C
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast4 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.727e-45
Identity = 87/250 (34.80%), Postives = 141/250 (56.40%), Query Frame = -2 
Query:   58 LKTIISFITLSVVIELCSAQCGCTQKGIKI-----SNFNVGDMVLSSQDVLRINGFKA--AQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATV-VLGGGCSANVGYN--KNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCN-GKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 774
            ++ +I  +   VVI+  + QCGC ++ +K+      N  +GDM+L S D+  INGFK   A    WPN ++ YTF  ++P      +R+ L   +K+L NC++F E ++  Y  V     GC + +G     + PL++  P    ++  +KHEF H LGFMH H R DRD  + I++  + +  C+ F  C+GC+    Y+ +S+M Y SE F+CN  +  ++KKNGG I +N E T  D + ++++Y C
Sbjct:    1 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 250
[Close]
BLAST of Ast4 protein vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 6.940e-44
Identity = 89/248 (35.89%), Postives = 131/248 (52.82%), Query Frame = -2 
Query:   58 LKTIISFITLSVVIELCSAQCGCTQKGI---KISNFNVGDMVLSSQDVLRINGFKAAQ--GVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATV-VLGGGCSANVGYNKNYPLSITLPDP-CVNRR-NVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSC-NGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 774
            ++  +  I L +  E+ S QCGC +K +      N +VGDM+LS  D+  INGFK  Q     WP   + + F   +P   +N +++ L   + +LGNC++F E     +  V     GC A +G        + L +P C+N +  +KHEF H LGFMH H R DRD F+ I + N+ S  C NF  CEGC +   Y+  S+M Y S+AF C  G +T+++KNG  I +N   T  D   ++  Y C
Sbjct:    5 MQKFLILIVLVLANEVAS-QCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 251
[Close]
BLAST of Ast4 protein vs. Ensembl Cavefish
Match: hce2l2 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103038533])

HSP 1 Score: 87.8113 bits (216), Expect = 8.688e-20
Identity = 54/180 (30.00%), Postives = 92/180 (51.11%), Query Frame = -2 
Query:   58 LRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVGYNKNYP-LSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKN--GGKIPFNMEVTKLDAKKVRDYYGC 585
            + YT  P Y +++K  I++ +++ E++   C+R+V       +  +    GC + VG N     LS+  P+ C+    V HE  H LGF+HE +R DRD ++ I + N+      NF   +   +   YD+ SIM +   A+S +G  TI++K    GK+  +   ++LD  K+   Y C
Sbjct:  117 IAYTLSPLYSEEDKMKIKQGMELIERK--TCVRYVPRTHQRDHLDIQPRSGCWSYVGANGGRQVLSLQTPE-CIGSAVVAHELMHALGFLHEQSRADRDNYVTILWSNIWKDRTRNFAKFKTNNMDMPYDYKSIMHFGKYAYSEDGNPTIVQKQNWNGKLGQSFGPSELDILKINKLYQC 293
[Close]
BLAST of Ast4 protein vs. Ensembl Cavefish
Match: ENSAMXT00000035014.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 85.1149 bits (209), Expect = 4.966e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = -2 
Query:   58 CLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 495
            C+RFV+  +++ Y ++    GC +++G      +       CV    V+HE  H LGF HEHTR DRD+++ I + N+   +  NF       L T YD+ S+M Y   AF+ NG +TI  +     +I   ++++ +D +++   Y C
Sbjct:   99 CIRFVDRSTEIDYLSIENRDGCYSSLGRTGGKQVVSLSTRGCVYHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTLYDYSSVMHYGKTAFTINGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 247
[Close]
BLAST of Ast4 protein vs. Ensembl Cavefish
Match: npsn (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191946])

HSP 1 Score: 85.5001 bits (210), Expect = 5.825e-19
Identity = 64/213 (30.05%), Postives = 101/213 (47.42%), Query Frame = -2 
Query:  139 ITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSS--QDVLRINGFKAAQGVHWP-----NKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRF-VEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTIL 753
            +T+S +IE  +   G T +   I     GD+++    Q+  R    K +    WP     N  + Y     +  K K+ I   LK +EK    C+RF   ++ + Y  V    GC + VG              C++++ ++HE  H LGF HE  R DRDQ ++I + N+  G  +NF   +   L T YD+ S+M Y  +AFS NG+ T++
Sbjct:   44 LTVSTIIEKANRNIGQTPEDPTI---RFGDILVDDGLQNADRCTSRKPS--CMWPRNSDGNTYIPYIISNQFRPKQKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQSLEGCYSFVGRTGGGQTLSLNRRGCLHKKIIQHELLHALGFHHEQCRSDRDQHIRILYENIIPGEEHNFDKEDTNNLNTPYDYGSVMHYERDAFSRNGQPTMV 249
[Close]
BLAST of Ast4 protein vs. Ensembl Cavefish
Match: ENSAMXT00000033223.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 85.1149 bits (209), Expect = 5.919e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = -2 
Query:   58 CLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 495
            C+RFV+  +++ Y ++    GC +++G      +       CV    V+HE  H LGF HEHTR DRD+++ I + N+   +  NF       L T YD+ S+M Y   AF+ NG +TI  +     +I   ++++ +D +++   Y C
Sbjct:  107 CIRFVDRSTEIDYLSIENRDGCYSSLGRTGGKQVVSLSTRGCVYHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTINGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255
[Close]
BLAST of Ast4 protein vs. Ensembl Cavefish
Match: ENSAMXT00000056363.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 85.1149 bits (209), Expect = 5.919e-19
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = -2 
Query:   58 CLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 495
            C+RFV+  +++ Y ++    GC +++G      +       CV    V+HE  H LGF HEHTR DRD+++ I + N+   +  NF       L T YD+ S+M Y   AF+ NG +TI  +     +I   ++++ +D +++   Y C
Sbjct:  107 CIRFVDRSTEIDYLSIENRDGCYSSLGRTGGKQVVSLSTRGCVYHGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTINGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255
[Close]
BLAST of Ast4 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.9593 bits (206), Expect = 2.387e-18
Identity = 63/193 (32.64%), Postives = 92/193 (47.67%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLG---GGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCK---LTTTYDFDSIMQYPSEAFSCN-GKDTILKK--NGGKIPFNMEVTKL---DAKKVRDYYGC 600
            WP  ++ Y    ++    K   R+ ++ +EK    C+ F+E ++D  + +V      GC + VG     P +I++   C     V HE  H++GF HEHTRPDRD  + I   N+  G   NF   E  +   L  +YDFDSIM Y    FS     DTIL +  + G  P   + T+L   D  + R  Y C
Sbjct:   22 WPGGVIPYIVGGNFTGSQKAMFRQAMRHWEKH--TCVTFIE-RTDEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFSRGIFLDTILPRPDDSGVRPAIGQRTRLSQGDIAQARKLYRC 211
[Close]
BLAST of Ast4 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 2.496e-9
Identity = 29/95 (30.53%), Postives = 40/95 (42.11%), Query Frame = -2 
Query:   94 CVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGC-KLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTK 375
            C     V HE  H +G++HE +RPDRD F+ +  GNV  G    F +          YD  S+M Y     +C  +D       G +    EV  
Sbjct:   21 CEGVGTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRACGTRDRPCGIPAGIVRCECEVCS 115
[Close]
BLAST of Ast4 protein vs. Ensembl Nematostella
Match: EDO39627 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9K8])

HSP 1 Score: 112.464 bits (280), Expect = 1.663e-29
Identity = 73/210 (34.76%), Postives = 108/210 (51.43%), Query Frame = -2 
Query:   58 GDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEE-KSDVYATVVL-GGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLT---TTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNME-VTKLDAKKVRDYYGC 669
            GD+VL+ +   ++N   A +  HW N ++ Y  DPS    +         I E     C+RF     SD Y   +  GGGC + VG +      ++L   C ++  V HEF H LGF HE +RPDRDQF++I+  N+  G+ +NF       +T   T YD+ S+M Y + AFS NGK TI+ K  G I      ++ +D K++  +Y C
Sbjct:   27 GDIVLTDEQKTQLNSRAAIKTNHWTNGVVPYELDPSISSSSYAVSVINSAIAEYNQKTCIRFRPRVPSDKYGLKIFKGGGCWSYVGMSMP-TQQLSLAGGCWSKGIVIHEFAHALGFYHEQSRPDRDQFVEIKTENIRDGVASNFKKHSFNYVTTHGTPYDYGSVMHYGAYAFSKNGKPTIVAKQPGVILGQRRGLSDIDTKQINIHYRC 235
[Close]
BLAST of Ast4 protein vs. Ensembl Nematostella
Match: EDO33956 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQR8])

HSP 1 Score: 108.997 bits (271), Expect = 3.127e-28
Identity = 70/208 (33.65%), Postives = 104/208 (50.00%), Query Frame = -2 
Query:   58 VLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD-VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNF----YLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 660
              SSQD  R           WPN ++ Y FD S  +K +  +     + E E   C+RFV+  ++  Y +   G GC  +VG+  NY   I++ D C  +  + HE  H +GF HE +RPDRD ++++ + NV  GL + F    +  E     + YDF SIM YP  AFS NG+ TI  +    GK P+ + ++  DA +    Y C
Sbjct:   30 AASSQDKTR-----------WPNAVIPYEFDCSIGNK-QECLSTSQSMAEWESKTCIRFVKRTTETAYLSFFRGQGCWGHVGHTANYKSQISIGDNCDFQHVMTHEIGHSVGFWHEQSRPDRDNYIQVLWENVVDGLHDAFEKRKWGTEVTDYGSKYDFASIMHYPFTAFSKNGRPTIKAIADMQGKTPY-VALSADDAMQTNAMYKC 224
[Close]
BLAST of Ast4 protein vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 107.457 bits (267), Expect = 6.137e-28
Identity = 72/218 (33.03%), Postives = 104/218 (47.71%), Query Frame = -2 
Query:   58 GDMVLSSQDVLR------INGFKAAQGV---HWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD-VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGC---KLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFN-MEVTKLDAKKVRDYYGC 669
            GDM L+    L       I+   A  G+    WP  ++ YT D S  ++  N IR  +K++      C+RF E  ++  YA    G GCS+ VG        I+L   C     V HE  H LGF HE +RPDRD ++ I + N+   +  NF +        L T YD+ S+M Y   AFS NG+ TI  K  G    N  +++K+D +++   Y C
Sbjct:    9 GDMRLTDAQRLAAQMGQDIDSPSARGGIKTGRWPEGVMAYTIDRSLSNRAMNAIRAGMKMWTDN--TCIRFKERTNEYAYARFWRGRGCSSMVG-RTGRRQDISLAGGCWYPGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQQMNLMYKC 223
[Close]
BLAST of Ast4 protein vs. Ensembl Nematostella
Match: EDO34806 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNB7])

HSP 1 Score: 103.605 bits (257), Expect = 5.254e-27
Identity = 60/160 (37.50%), Postives = 87/160 (54.37%), Query Frame = -2 
Query:   58 IYEKELGNCLRFVEEKSD--VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLT---TTYDFDSIMQYPSEAFSCNGKDTIL-KKNGGKIPFNMEVTKLDAKKVRDYYGC 519
            I E     C+R   +K+    Y ++  GGGC + VG   +    ++L   C ++  V HE  H LGF HE +RPDRD F+ I+F N+  G   NF +    ++T   TTYDF+S+M Y   AFS NG+ TI+ KK G  +     ++ LD  +V  +YGC
Sbjct:    7 IAEYAQKTCIRLRPKKAGETAYLSIFKGGGCWSYVGRTGS-KQQLSLAGGCWHKGIVIHEIAHALGFFHEQSRPDRDHFVTIKFENIQDGKEGNFRMHSTSQVTTHGTTYDFESVMHYGGRAFSKNGQPTIVAKKAGVNLGQRHGLSHLDTIQVNIHYGC 165
[Close]
BLAST of Ast4 protein vs. Ensembl Nematostella
Match: EDO33955 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQR7])

HSP 1 Score: 104.76 bits (260), Expect = 7.057e-27
Identity = 63/188 (33.51%), Postives = 96/188 (51.06%), Query Frame = -2 
Query:   58 WPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD-VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNV----HSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 600
            WPN ++ Y FD S  +K +  +     + E E   C+RFV+  ++  Y +   G GC  ++G+  NY   I++ D C  +  + HE  H +GF HEH RPDRD ++++ + NV    H       +  E     + YDF SIM YP  AFS NG+ TI  +    GK P+ + ++  DA +    Y C
Sbjct:    1 WPNAVIPYEFDCSIGNK-QECLSTSQSMAEWESKTCIRFVKRTTETAYLSFFRGQGCWGHLGHTANYKSQISIGDNCDFQYVMSHEIGHSVGFWHEHNRPDRDNYIQVLWENVVDEFHDAFEKRKWGTEVTDYGSQYDFASIMHYPFTAFSKNGRPTIKAIADMQGKTPY-VALSADDAMQTNAMYKC 186
[Close]
BLAST of Ast4 protein vs. Ensembl Medaka
Match: alv (alveolin [Source:NCBI gene;Acc:100049214])

HSP 1 Score: 88.9669 bits (219), Expect = 2.022e-20
Identity = 61/204 (29.90%), Postives = 98/204 (48.04%), Query Frame = -2 
Query:   58 DMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 666
            D+ ++  D+L ++  + A  + W   ++ YT       + ++ I+E   +       C+RF E   +  Y  +  G GC++ VG +     S+     C +  N+ HE  H +G  HEHTR DRDQF+ + + N+  G   NF +  G  L   YDF SIM Y +  FS +G  TI    G  I     +++LD +K+   Y C
Sbjct:   53 DLSVAEGDIL-LSDDRNAVNMLWAQGVIPYTISMELAHR-QSEIKEAFSMITS--ATCIRFQERTYEANYLDIKDGDGCASYVGCSGG-AQSVYFGSTC-SVGNLCHELVHAVGMYHEHTRYDRDQFISVIWDNIKPGKQGNFDVKMGDTLNLPYDFGSIMHYGTSYFSKDGSPTIQPNEGVNIGQRKRLSQLDIQKINTLYRC 250
[Close]
BLAST of Ast4 protein vs. Ensembl Medaka
Match: alv (alveolin [Source:NCBI gene;Acc:100049214])

HSP 1 Score: 88.9669 bits (219), Expect = 2.100e-20
Identity = 61/204 (29.90%), Postives = 98/204 (48.04%), Query Frame = -2 
Query:   58 DMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 666
            D+ ++  D+L ++  + A  + W   ++ YT       + ++ I+E   +       C+RF E   +  Y  +  G GC++ VG +     S+     C +  N+ HE  H +G  HEHTR DRDQF+ + + N+  G   NF +  G  L   YDF SIM Y +  FS +G  TI    G  I     +++LD +K+   Y C
Sbjct:   64 DLSVAEGDIL-LSDDRNAVNMLWAQGVIPYTISMELAHR-QSEIKEAFSMITS--ATCIRFQERTYEANYLDIKDGDGCASYVGCSGG-AQSVYFGSTC-SVGNLCHELVHAVGMYHEHTRYDRDQFISVIWDNIKPGKQGNFDVKMGDTLNLPYDFGSIMHYGTSYFSKDGSPTIQPNEGVNIGQRKRLSQLDIQKINTLYRC 261
[Close]
BLAST of Ast4 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 86.6557 bits (213), Expect = 1.864e-19
Identity = 62/213 (29.11%), Postives = 102/213 (47.89%), Query Frame = -2 
Query:   58 GDMVL-SSQDVLRINGFKAAQGVHWP---NKLLR--YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD-VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 669
            GD+VL  +++ ++   F A     WP   N +++  Y    +Y    K  IR  +K + ++   C+ FV   ++  Y ++    GC + +GY  +  + +     C+    ++HE  H LGF HEHTR DRDQ +KI + N+     +NF   +   L T YD+ SIM Y   AF  + K+TI  +      I     ++ +D  +V   Y C
Sbjct:   63 GDLVLPKTRNAMKC--FGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQRFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast4 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 86.6557 bits (213), Expect = 1.882e-19
Identity = 62/213 (29.11%), Postives = 102/213 (47.89%), Query Frame = -2 
Query:   58 GDMVL-SSQDVLRINGFKAAQGVHWP---NKLLR--YTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSD-VYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTI--LKKNGGKIPFNMEVTKLDAKKVRDYYGC 669
            GD+VL  +++ ++   F A     WP   N +++  Y    +Y    K  IR  +K + ++   C+ FV   ++  Y ++    GC + +GY  +  + +     C+    ++HE  H LGF HEHTR DRDQ +KI + N+     +NF   +   L T YD+ SIM Y   AF  + K+TI  +      I     ++ +D  +V   Y C
Sbjct:   63 GDLVLPKTRNAMKC--FGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQRFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast4 protein vs. Ensembl Medaka
Match: mnep (nephrosin [Source:NCBI gene;Acc:100049315])

HSP 1 Score: 84.7297 bits (208), Expect = 8.467e-19
Identity = 59/195 (30.26%), Postives = 89/195 (45.64%), Query Frame = -2 
Query:   58 AAQGVHWP-----NKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDV-YATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFN--MEVTKLDAKKVRDYYGC 618
             A+G  WP     N  + +     +  + ++ I + L+ +E     C+RF   +S   +  +    GC + VG      +   +   CV    ++HE  H LGF HE TR DRD  ++I   NV SG  +NF   E   L T YD++SIM Y   AFS N + TI+      +P     E++  D  +V   Y C
Sbjct:   81 TARGCLWPKSSDGNVYVPFRISGQFSSRERDTILQGLRSFEGS--TCIRFTPHQSQRDFVDIQSRTGCWSFVGRRGGGQVVSLMRQGCVFMGTIQHELLHALGFNHEQTRSDRDSHVRILLQNVISGQEHNFRKIETRNLGTPYDYNSIMHYGRFAFSRNREPTIVPIPDPNVPIGRATEMSSNDILRVNRLYEC 273
[Close]
BLAST of Ast4 protein vs. Planmine SMEST
Match: SMESG000080471.1 (SMESG000080471.1)

HSP 1 Score: 496.893 bits (1278), Expect = 3.056e-180
Identity = 238/241 (98.76%), Postives = 239/241 (99.17%), Query Frame = -2 
Query:   58 MLLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 780
            M LKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKN IREFLKIYEKELGNCLRF+EEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC
Sbjct:    1 MTLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNAIREFLKIYEKELGNCLRFLEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 241
[Close]
BLAST of Ast4 protein vs. Planmine SMEST
Match: SMESG000080475.1 (SMESG000080475.1)

HSP 1 Score: 387.111 bits (993), Expect = 6.632e-137
Identity = 180/241 (74.69%), Postives = 202/241 (83.82%), Query Frame = -2 
Query:   58 MLLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 780
            M+LK ++    L  +  LC AQCGCTQKGIKI++FNVGDMVLS  ++L+ING+KAA G  WP   LRYTFDPS+PDKNKNP+R+FLK YEKELGNC++F EEKS V+ATVV GGGCSANVGYN  YPL +TL DPCVN+RNVKHEFTH LGF HEHTRPDRD  L I+F NV   LC NFYLCEGCK TT YDFDSIMQYPSEAF+CNGK+TIL+KNGGKI FNMEVTKLDAKKVRDYYGC
Sbjct:    1 MILKIVVICFLLITLFSLCYAQCGCTQKGIKIADFNVGDMVLSPVEILKINGYKAADGEKWPKNELRYTFDPSFPDKNKNPVRDFLKAYEKELGNCIKFKEEKSGVFATVVTGGGCSANVGYNSRYPLKVTLADPCVNKRNVKHEFTHSLGFQHEHTRPDRDNNLNIQFNNVKKNLCANFYLCEGCKTTTPYDFDSIMQYPSEAFTCNGKETILRKNGGKIAFNMEVTKLDAKKVRDYYGC 241
[Close]
BLAST of Ast4 protein vs. Planmine SMEST
Match: SMESG000016662.1 (SMESG000016662.1)

HSP 1 Score: 372.474 bits (955), Expect = 4.089e-131
Identity = 171/241 (70.95%), Postives = 202/241 (83.82%), Query Frame = -2 
Query:   58 MLLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRINGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 780
            M ++ +  FI L V IELC +QCGC+QKGIK++NFN+ DM+LS +D+LRINGFKAAQ   WP+K  RY FDPSYPD+NKN IRE+LKIYE ELGNCL+F E+KSDV+ATV++G GCS+++GY+KN  L ITLPD CVN+RNVKHEFTHLLGFMHEHTRPDRDQ+LKI   NV    C+NF+LCEGCK +T YDFDSIMQYPS+AF+CNGK+TILK NG KI  NMEVTKLD KKVRDYY C
Sbjct:    1 MFVENLFCFIILGVGIELCYSQCGCSQKGIKVTNFNIDDMILSEKDILRINGFKAAQAALWPDKTARYIFDPSYPDRNKNRIREYLKIYEGELGNCLKFYEDKSDVHATVIMGKGCSSSIGYDKNKELRITLPDVCVNKRNVKHEFTHLLGFMHEHTRPDRDQYLKILLENVSPAYCHNFFLCEGCKTSTPYDFDSIMQYPSKAFTCNGKETILKINGDKIRSNMEVTKLDVKKVRDYYSC 241
[Close]
BLAST of Ast4 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 172.555 bits (436), Expect = 1.655e-52
Identity = 91/248 (36.69%), Postives = 140/248 (56.45%), Query Frame = -2 
Query:   58 LKTIISFITLSVVIELCSAQCGC-TQKGIK--ISNFNVGDMVLSSQDVLRINGFKA--AQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATV-VLGGGCSANVGYN--KNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSC-NGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 774
            ++++   + +   +   S QCGC  QK +K  + +  +GDMVL   D+  INGFKA  +    WPN  + ++F P++P   K  +RE L   +K+LGNC++F E  +  Y  V  L  GC + +GY    N PL++  P    ++  VKHEF H LGFMH H R DRD  + I++  + +  C+ F  CEGC L   Y+ +S+M YPS  F+C  G++ + K++GG I +N   ++ D   VR +Y C
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast4 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 159.844 bits (403), Expect = 5.782e-48
Identity = 83/213 (38.97%), Postives = 122/213 (57.28%), Query Frame = -2 
Query:   58 FNVGDMVLSSQDVLRINGFKA--AQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFVEEKSDVYATV-VLGGGCSANVGYN--KNYPLSITLPDPCVNRRNVKHEFTHLLGFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQYPSEAFSC-NGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC 678
              +GDMVL   D+  INGFKA  +    WPN  + ++F P++P   K  +RE L   +K+LGNC++F E  +  Y  V  L  GC + +GY    N PL++  P    ++  VKHEF H LGFMH H R DRD  + I++  + +  C+ F  CEGC L   Y+ +S+M YPS  F+C  G++ + K++GG I +N   ++ D   VR +Y C
Sbjct:    1 MQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 213
The following BLAST results are available for this feature:
BLAST of Ast4 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TLL21.992e-1831.96tolloid like 2 [Source:HGNC Symbol;Acc:HGNC:11844][more]
TLL14.196e-1832.64tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
BMP14.730e-1832.64bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
BMP15.922e-1832.64bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
BMP17.369e-1832.64bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
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BLAST of Ast4 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-143.131e-2130.88Zinc metalloproteinase nas-14 [Source:UniProtKB/S... [more]
nas-71.976e-1929.95Zinc metalloproteinase nas-7 [Source:UniProtKB/Sw... [more]
nas-12.136e-1931.22Zinc metalloproteinase nas-1 [Source:UniProtKB/Sw... [more]
nas-121.911e-1829.96Zinc metalloproteinase nas-12 [Source:UniProtKB/S... [more]
nas-152.289e-1833.16Zinc metalloproteinase nas-15 [Source:UniProtKB/S... [more]
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BLAST of Ast4 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG118658.791e-2327.24gene:FBgn0028947 transcript:FBtr0080753[more]
CG67632.879e-2129.68gene:FBgn0039069 transcript:FBtr0084378[more]
CG67632.879e-2129.68gene:FBgn0039069 transcript:FBtr0334497[more]
CG152533.074e-1931.51gene:FBgn0028948 transcript:FBtr0080782[more]
CG152543.740e-1828.69gene:FBgn0028949 transcript:FBtr0343718[more]
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BLAST of Ast4 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CU326366.28.920e-1933.51pep chromosome:GRCz11:10:42887204:42898220:-1 gene... [more]
hce2l23.925e-1828.96high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
bmp1a4.064e-1831.77bone morphogenetic protein 1a [Source:ZFIN;Acc:ZDB... [more]
bmp1a4.076e-1831.77bone morphogenetic protein 1a [Source:ZFIN;Acc:ZDB... [more]
tll12.006e-1732.47tolloid-like 1 [Source:NCBI gene;Acc:474335][more]
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BLAST of Ast4 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
golt1b4.411e-2132.58golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
golt1b6.067e-2132.58golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
golt1b3.896e-2032.58golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
golt1b7.428e-2032.02golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
golt1b8.645e-2032.02golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
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BLAST of Ast4 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tll21.210e-1832.47tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044][more]
Tll27.432e-1833.55tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044][more]
Tll12.882e-1732.99tolloid-like [Source:MGI Symbol;Acc:MGI:106923][more]
Bmp13.204e-1732.64bone morphogenetic protein 1 [Source:MGI Symbol;Ac... [more]
Astl8.767e-1526.26astacin-like metalloendopeptidase (M12 family) [So... [more]
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BLAST of Ast4 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q19269|NAS14_CAEEL3.983e-2030.88Zinc metalloproteinase nas-14 OS=Caenorhabditis el... [more]
sp|P31579|LCE_ORYLA1.670e-1829.11Low choriolytic enzyme OS=Oryzias latipes OX=8090 ... [more]
sp|P55113|NAS7_CAEEL2.514e-1829.95Zinc metalloproteinase nas-7 OS=Caenorhabditis ele... [more]
sp|O62243|NAS1_CAEEL2.717e-1831.22Zinc metalloproteinase nas-1 OS=Caenorhabditis ele... [more]
sp|Q9WVM6|TLL2_MOUSE8.466e-1832.47Tolloid-like protein 2 OS=Mus musculus OX=10090 GN... [more]
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BLAST of Ast4 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A060Q6Y61.099e-17698.76Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7152.357e-13175.85Metalloendopeptidase (Fragment) OS=Schmidtea medit... [more]
A0A060Q7865.950e-4936.69Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7142.727e-4534.80Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
I1ZIC36.940e-4435.89Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
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BLAST of Ast4 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l28.688e-2030.00low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000035014.14.966e-1930.87high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
npsn5.825e-1930.05low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000033223.15.919e-1930.87high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
ENSAMXT00000056363.15.919e-1930.87high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
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BLAST of Ast4 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007253.12.387e-1832.64pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
ENSPMAT00000004913.12.496e-930.53pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
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BLAST of Ast4 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ast4 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO396271.663e-2934.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO339563.127e-2833.65Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414206.137e-2833.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348065.254e-2737.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO339557.057e-2733.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ast4 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alv2.022e-2029.90alveolin [Source:NCBI gene;Acc:100049214][more]
alv2.100e-2029.90alveolin [Source:NCBI gene;Acc:100049214][more]
lce1.864e-1929.11protease [Source:NCBI gene;Acc:100049451][more]
lce1.882e-1929.11protease [Source:NCBI gene;Acc:100049451][more]
mnep8.467e-1930.26nephrosin [Source:NCBI gene;Acc:100049315][more]
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BLAST of Ast4 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000080471.13.056e-18098.76SMESG000080471.1[more]
SMESG000080475.16.632e-13774.69SMESG000080475.1[more]
SMESG000016662.14.089e-13170.95SMESG000016662.1[more]
SMESG000072791.11.655e-5236.69SMESG000072791.1[more]
SMESG000072791.15.782e-4838.97SMESG000072791.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30019357
GenBankCCW36842.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019357 ID=SMED30019357|Name=Ast4 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=898bp
AAACAATGCTAAGGTATTTATTTATATCTGTTTAATATTCATTTAATCAT
GTTGTTAGCATCCGTAGTAGTCTCGGACTTTCTTTGCGTCCAATTTTGTT
ACTTCCATATTGAATGGGATTTTTCCTCCATTTTTCTTCAAAATTGTGTC
TTTTCCATTGCAAGAGAAAGCTTCCGATGGATATTGCATGATACTGTCAA
AATCATAAGTTGTTGTCAATTTGCATCCTTCACACAAATAGAAGTTATTG
CATAATCCTGAATGAACATTTCCGAATTCTATTTTCAAAAATTGGTCTCT
GTCCGGTCGAGTGTGCTCATGCATAAATCCGAGTAAATGAGTGAATTCGT
GCTTGACATTTCTTCTATTGACACATGGATCAGGCAAAGTAATTGACAGA
GGATAATTTTTATTGTATCCAACATTGGCTGAGCATCCTCCACCCAAAAC
TACAGTGGCGTAAACATCACTCTTTTCCTCTACGAATCTCAAACAGTTTC
CCAATTCCTTTTCATAGATCTTCAAGAATTCTCTGATTGGATTTTTGTTT
TTATCGGGATATGAGGGATCAAATGTATATCTGAGCAATTTGTTTGGCCA
ATGAACTCCTTGAGCAGCTTTGAATCCGTTTATTCTCAAAACGTCTTGAG
ATGACAAAACCATATCCCCGACATTGAAATTGGATATTTTAATTCCCTTT
TGAGTGCAACCGCATTGAGCACTGCACAGTTCAATTACGACTGAAAGAGT
TATGAAAGAAATAATAGTTTTCAACAACATTGTTATGAATAAATGTATTT
TGTGACGGCATTTTATGTAGTTTGCACCGGATGTGGAAACGATCTAACCC
ATTAGAGTTATATTTATTGATCAATTTAATACAATTTCTTGATCGGAC
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protein sequence of SMED30019357-orf-1

>SMED30019357-orf-1 ID=SMED30019357-orf-1|Name=SMED30019357-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=242bp
MLLKTIISFITLSVVIELCSAQCGCTQKGIKISNFNVGDMVLSSQDVLRI
NGFKAAQGVHWPNKLLRYTFDPSYPDKNKNPIREFLKIYEKELGNCLRFV
EEKSDVYATVVLGGGCSANVGYNKNYPLSITLPDPCVNRRNVKHEFTHLL
GFMHEHTRPDRDQFLKIEFGNVHSGLCNNFYLCEGCKLTTTYDFDSIMQY
PSEAFSCNGKDTILKKNGGKIPFNMEVTKLDAKKVRDYYGC*
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Aliases
The feature 'Ast4 protein' has the following synonyms
Synonym
Ast4
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001506Peptidase M12APRINTSPR00480ASTACINcoord: 191..206
score: 49.27
coord: 227..240
score: 21.9
coord: 155..172
score: 46.02
coord: 136..154
score: 41.14
IPR001506Peptidase M12APFAMPF01400Astacincoord: 60..241
e-value: 1.6E-33
score: 115.9
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 57..196
e-value: 2.2E-7
score: 40.5
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 21..241
e-value: 6.7E-47
score: 161.9
NoneNo IPR availablePANTHERPTHR10127DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAININGcoord: 38..241
NoneNo IPR availablePROSITEPS51864ASTACINcoord: 55..241
score: 31.349
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.851
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 56..241