Myosin regulatory light chain

Overview


Name	Myosin regulatory light chain
Smed ID	SMED30019325
Length (bp)	1094

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Myosin regulatory light chain (SMED30019325) t-SNE clustered cells

Violin plots show distribution of expression levels for Myosin regulatory light chain (SMED30019325) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myosin regulatory light chain (SMED30019325) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myosin regulatory light chain (SMED30019325) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019325

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 20

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30019325	h1SMcG0013220	Contig246	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30019325	h1SMcG0013220	Contig246	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30019325	h1SMcG0013220	Contig246	GPL15192	PMID:23079596 Forsthoefel et al., 2012
pharynx	SMED30019325	h1SMcG0013220	Contig2068	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30019325	h1SMcG0013220	Contig2068	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30019325	h1SMcG0013220	Contig2068	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30019325	h1SMcG0013220	Contig3175	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30019325	h1SMcG0013220	Contig3175	GPL15192	PMID:23079596 Forsthoefel et al., 2012
Smed sexual biotype	SMED30019325	h1SMcG0013220	Contig39702	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019325	h1SMcG0013220	Contig39702	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30019325	h1SMcG0013220	dd_Smed_v4_383_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30019325	h1SMcG0013220	dd_Smed_v6_383_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
Category 3 cell	SMED30019325	h1SMcG0013220	dd_Smed_v6_383_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
parenchymal cell	SMED30019325	h1SMcG0013220	dd_Smed_v6_383_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 216.468 bits (550), Expect = 2.534e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-4 (Myosin regulatory light chain [Source:UniProtKB/Swiss-Prot;Acc:Q09510])

HSP 1 Score: 223.787 bits (569), Expect = 1.257e-72
Identity = 107/171 (62.57%), Postives = 138/171 (80.70%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGT--INFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            MAS KT  + R R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK+  E +I+ M+ +A G   INFTMFLTLFGEK+TG+DPEE I NAF CFD +++G ++EEHLR+L+T+MG+R+++EQVDELF  API  G+F+Y EFT+M+KHG K++D
Sbjct:    1 MASRKTVNR-RQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASLGKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLNEEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKDKD 170

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-1 (Myosin regulatory light chain 1 [Source:UniProtKB/Swiss-Prot;Acc:P19625])

HSP 1 Score: 133.65 bits (335), Expect = 1.117e-37
Identity = 66/148 (44.59%), Postives = 100/148 (67.57%), Query Frame = 3

Query:  171 SMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHL-RDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEE 608
            + F +  IQEFKEAF ++DQNKDG ID  DL ++  S+G+ A ++ I+ M+K+A G INFT+FLTLFGE++TG+DPE TI+ AFA FD +D G + E+ L + L    G+   +++V  ++ G  PI  G+ +Y+ F  +I  G ++E
Sbjct:   19 AQFDQKTIQEFKEAFGIMDQNKDGIIDKSDLKDLYASMGQIAPDSQIDAMIKEASGPINFTVFLTLFGERLTGTDPEATIIGAFAMFDKKDCGKIKEDDLIKILQNKRGEPLDEDEVKAMYKGKPPIEGGEVDYKAFAHLITTGAQDE 166

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-2 (Myosin regulatory light chain 2 [Source:UniProtKB/Swiss-Prot;Acc:P19626])

HSP 1 Score: 133.265 bits (334), Expect = 1.337e-37
Identity = 66/148 (44.59%), Postives = 100/148 (67.57%), Query Frame = 3

Query:  171 SMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHL-RDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEE 608
            + F +  IQEFKEAF ++DQNKDG ID  DL ++  S+G+ A ++ I+ M+K+A G INFT+FLTLFGE++TG+DPE TI+ AFA FD +D G + E+ L + L    G+   +++V  ++ G  PI  G+ +Y+ F  +I  G ++E
Sbjct:   19 AQFDQKTIQEFKEAFGIMDQNKDGIIDKSDLKDLYASMGQIAPDSQIDAMIKEASGPINFTVFLTLFGERLTGTDPEATIVGAFAMFDKKDCGKIKEDDLIKILQNKRGEPLDEDEVKAMYKGKPPIEGGEVDYKAFAHLITTGAQDE 166

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 109.383 bits (272), Expect = 8.948e-29
Identity = 59/142 (41.55%), Postives = 89/142 (62.68%), Query Frame = 3

Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 146

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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 85.5001 bits (210), Expect = 2.640e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 3

Query:  273 LTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
            + SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    1 MRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 110

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BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339504)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3

Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339503)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3

Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0070877)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3

Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339502)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3

Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0345294)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3

Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172

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BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl12.2 (myosin, light chain 12, genome duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9028])

HSP 1 Score: 221.861 bits (564), Expect = 1.054e-71
Identity = 108/169 (63.91%), Postives = 135/169 (79.88%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIKNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 171

[Close]

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIKNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 221.476 bits (563), Expect = 2.182e-71
Identity = 107/169 (63.31%), Postives = 135/169 (79.88%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + TGF+ E+HLRDL+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 172

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Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + TGF+ E+HLRDL+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 172

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Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + TGF+ E+HLRDL+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 172

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl9 (myosin light chain 9 [Source:NCBI gene;Acc:100124811])

HSP 1 Score: 218.009 bits (554), Expect = 5.203e-70
Identity = 101/158 (63.92%), Postives = 131/158 (82.91%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+GK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E +GF++E+HLR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG KE+D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFINEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKEKD 171

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl12b (myosin light chain 12B [Source:NCBI gene;Acc:448060])

HSP 1 Score: 212.616 bits (540), Expect = 6.603e-68
Identity = 100/158 (63.29%), Postives = 127/158 (80.38%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E  G + E+ LR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:   15 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGIGSIQEDTLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYNEFTRILKHGAKDKD 172

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: ap5z1 (adaptor related protein complex 5 subunit zeta 1 [Source:Xenbase;Acc:XB-GENE-6034870])

HSP 1 Score: 172.17 bits (435), Expect = 3.056e-52
Identity = 82/164 (50.00%), Postives = 117/164 (71.34%), Query Frame = 3

Query:  126 KKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEED 611
            KK + R +   SNVFSMF + QIQEFKEAF ++DQN+DGFID +DL +   +LG+ +     +EEMLK+A G INFT+FLT+FGEK+ G+DPEETILNAF  FDPE TG +  E++R+++ +  +R+T E+VD++F    P   G  NY+    +I HG+++E+
Sbjct:    4 KKAKKRAEGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRLNVKNEELEEMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGLLKSEYIREMLMTQAERFTSEEVDQMFTAFPPDVTGNLNYKNLVHIITHGEEKEE 167

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: ap5z1 (adaptor related protein complex 5 subunit zeta 1 [Source:Xenbase;Acc:XB-GENE-6034870])

HSP 1 Score: 174.481 bits (441), Expect = 4.621e-52
Identity = 83/170 (48.82%), Postives = 120/170 (70.59%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEED 611
            A+  + KK + R +   SNVFSMF + QIQEFKEAF ++DQN+DGFID +DL +   +LG+ +     +EEMLK+A G INFT+FLT+FGEK+ G+DPEETILNAF  FDPE TG +  E++R+++ +  +R+T E+VD++F    P   G  NY+    +I HG+++E+
Sbjct:   76 AANTSPKKAKKRAEGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRLNVKNEELEEMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGLLKSEYIREMLMTQAERFTSEEVDQMFTAFPPDVTGNLNYKNLVHIITHGEEKEE 245

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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: tnrc6c (trinucleotide repeat containing 6C [Source:Xenbase;Acc:XB-GENE-6044444])

HSP 1 Score: 157.147 bits (396), Expect = 2.595e-46
Identity = 78/157 (49.68%), Postives = 109/157 (69.43%), Query Frame = 3

Query:  147 QRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPIS-NGKFNYREFTKMIKHGKKEED 611
            QR +SNVFSMF + QIQEFKEAF+ IDQN+DG I   DL E    LGK + NE  ++EMLK+  G INFT+FL+LFGEK+ G+DPE++IL AF   DP  TG ++++ L+ L+ +  D++T E+VD++F   PI   G  +Y+    +I HG ++ED
Sbjct:   16 QRGSSNVFSMFEQSQIQEFKEAFSCIDQNRDGIITKSDLKETYMQLGKMNVNEDELDEMLKEGKGPINFTVFLSLFGEKLNGTDPEDSILAAFKILDPNATGNINKDELKQLLMTQADKFTAEEVDQMFAVTPIDVAGNIDYKSLCYIITHGDEKED 172

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Gm49909 (predicted gene, 49909 [Source:MGI Symbol;Acc:MGI:6270609])

HSP 1 Score: 216.468 bits (550), Expect = 1.739e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12b (myosin, light chain 12B, regulatory [Source:MGI Symbol;Acc:MGI:107494])

HSP 1 Score: 216.468 bits (550), Expect = 1.739e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl9 (myosin, light polypeptide 9, regulatory [Source:MGI Symbol;Acc:MGI:2138915])

HSP 1 Score: 214.927 bits (546), Expect = 6.812e-69
Identity = 100/156 (64.10%), Postives = 129/156 (82.69%), Query Frame = 3

Query:  147 QRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E +GF+ E+HLR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG K++D
Sbjct:   16 QRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12a (myosin, light chain 12A, regulatory, non-sarcomeric [Source:MGI Symbol;Acc:MGI:1914518])

HSP 1 Score: 211.46 bits (537), Expect = 1.833e-67
Identity = 98/158 (62.03%), Postives = 128/158 (81.01%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+GK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E  G + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEAIGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|Q09510|MLRH_CAEEL (Myosin regulatory light chain OS=Caenorhabditis elegans OX=6239 GN=mlc-4 PE=1 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 1.598e-71
Identity = 107/171 (62.57%), Postives = 138/171 (80.70%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGT--INFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            MAS KT  + R R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK+  E +I+ M+ +A G   INFTMFLTLFGEK+TG+DPEE I NAF CFD +++G ++EEHLR+L+T+MG+R+++EQVDELF  API  G+F+Y EFT+M+KHG K++D
Sbjct:    1 MASRKTVNR-RQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASLGKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLNEEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKDKD 170

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|A4IF97|ML12B_BOVIN (Myosin regulatory light chain 12B OS=Bos taurus OX=9913 GN=MYL12B PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 1.333e-69
Identity = 106/170 (62.35%), Postives = 137/170 (80.59%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            M+S K + K + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:    1 MSSKKAKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 170

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P24032|MLRN_CHICK (Myosin regulatory light chain 2, smooth muscle minor isoform OS=Gallus gallus OX=9031 PE=2 SV=2)

HSP 1 Score: 217.238 bits (552), Expect = 6.704e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P18666|ML12B_RAT (Myosin regulatory light chain 12B OS=Rattus norvegicus OX=10116 GN=Myl12b PE=1 SV=3)

HSP 1 Score: 216.468 bits (550), Expect = 1.178e-68
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGRINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|Q3THE2|ML12B_MOUSE (Myosin regulatory light chain 12B OS=Mus musculus OX=10090 GN=Myl12b PE=1 SV=2)

HSP 1 Score: 216.468 bits (550), Expect = 1.217e-68
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

[Close]

BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A0X3Q2Q1 (Myosin regulatory light chain sqh OS=Schistocephalus solidus OX=70667 GN=SQH PE=4 SV=1)

HSP 1 Score: 260.381 bits (664), Expect = 2.565e-83
Identity = 118/169 (69.82%), Postives = 148/169 (87.57%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M++TK++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +EAY++EMLKQA G INFTMFLTLFGEKM G DPE++ILNAFACFDP +TG+++EE LR+LMT+MGDRWTD++VDELFHGAPI +G+FNYREFTKMI HG+ +E+
Sbjct:    1 MSATKSKKRGRARTQRYTSNVFSMFTEAQINEFKEAFSIIDGSKDGIIDQSDLEEVFRSMGKNPSEAYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEDSILNAFACFDPNNTGYINEERLRELMTTMGDRWTDDKVDELFHGAPIKDGEFNYREFTKMIMHGQDDEE 169

[Close]

BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A5K3EJQ3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 4.846e-83
Identity = 125/174 (71.84%), Postives = 148/174 (85.06%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNP 623
            MASTKT+K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+  EAY+EEMLKQA G INFTMFLTLFGEKM G DPE+TILNAFA FDP + G ++EE LR+LMT+MGDRWTDE+VDELFHGAPI +G+FNYREFTKMI HG++ +ED N+P
Sbjct:    1 MASTKTKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPTEAYLEEMLKQAPGCINFTMFLTLFGEKMMGCDPEDTILNAFAQFDPNNKGVINEERLRELMTTMGDRWTDEKVDELFHGAPIQDGEFNYREFTKMIIHGQQDDEDQNHP 174

[Close]

BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A087VX66 (Myosin regulatory light chain 2 smooth muscle OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000041600 PE=4 SV=1)

HSP 1 Score: 260.381 bits (664), Expect = 5.681e-83
Identity = 126/176 (71.59%), Postives = 148/176 (84.09%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNPNL 629
            MA  K++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +E Y++EMLKQA G INFTMFLTLFGEKM G DPEETILNAFA FDP +TG +SEE LRDLMT+MGDRWTDE+VDELFHGAPI NG FNYREFTKMI HG++ +ED N+P+L
Sbjct:    1 MAFAKSKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPSEEYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEETILNAFAQFDPNNTGVISEERLRDLMTTMGDRWTDEKVDELFHGAPIKNGDFNYREFTKMIMHGQQDDEDQNHPSL 176

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BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A068X1P7 (Myosin regulatory light chain 2 smooth muscle OS=Echinococcus granulosus OX=6210 GN=EgrG_000041600 PE=4 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 6.985e-83
Identity = 126/176 (71.59%), Postives = 148/176 (84.09%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNPNL 629
            MA  K++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +E Y++EMLKQA G INFTMFLTLFGEKM G DPEETILNAFA FDP +TG +SEE LRDLMT+MGDRWTDE+VDELFHGAPI NG FNYREFTKMI HG++ +ED N+P+L
Sbjct:    1 MAFAKSKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPSEEYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEETILNAFAQFDPNNTGVISEERLRDLMTTMGDRWTDEKVDELFHGAPIKNGDFNYREFTKMIMHGQQDDEDQNHPSL 176

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BLAST of Myosin regulatory light chain vs. TrEMBL
Match: W6USV8 (Myosin regulatory light chain sqh OS=Echinococcus granulosus OX=6210 GN=EGR_00670 PE=4 SV=1)

HSP 1 Score: 258.455 bits (659), Expect = 1.249e-82
Identity = 125/174 (71.84%), Postives = 146/174 (83.91%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNP 623
            MA  K++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +E Y++EMLKQA G INFTMFLTLFGEKM G DPEETILNAFA FDP +TG +SEE LRDLMT+MGDRWTDE+VDELFHGAPI NG FNYREFTKMI HG++ +ED N+P
Sbjct:    1 MAFAKSKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPSEEYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEETILNAFAQFDPNNTGVISEERLRDLMTTMGDRWTDEKVDELFHGAPIKNGDFNYREFTKMIMHGQQDDEDQNHP 174

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000048105.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:9819022:9829334:-1 gene:ENSAMXG00000037886.1 transcript:ENSAMXT00000048105.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 219.935 bits (559), Expect = 1.089e-70
Identity = 107/169 (63.31%), Postives = 134/169 (79.29%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API   G FNY  FT+++KHG K++D
Sbjct:   24 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVAFTRILKHGAKDKD 189

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: myl9b (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:103045271])

HSP 1 Score: 219.164 bits (557), Expect = 1.106e-70
Identity = 106/169 (62.72%), Postives = 135/169 (79.88%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAFACFD E +G + E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDDYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKD 171

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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000046001.1 (myosin regulatory light chain 2, smooth muscle minor isoform-like [Source:NCBI gene;Acc:103039566])

HSP 1 Score: 218.779 bits (556), Expect = 1.433e-70
Identity = 106/169 (62.72%), Postives = 134/169 (79.29%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGF+D +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API     FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFVDKEDLHDMLASLGKNPTDDYLEAMMTEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKSNFNYVEFTRILKHGAKDKD 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: myl9a (myosin regulatory light polypeptide 9-like [Source:NCBI gene;Acc:103022187])

HSP 1 Score: 218.779 bits (556), Expect = 2.052e-70
Identity = 105/169 (62.13%), Postives = 135/169 (79.88%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + +GF+ E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDGSGFIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYTEFTRILKHGAKDKD 172

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000051744.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000067.1:2135412:2141173:-1 gene:ENSAMXG00000034506.1 transcript:ENSAMXT00000051744.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 162.54 bits (410), Expect = 6.910e-49
Identity = 79/156 (50.64%), Postives = 113/156 (72.44%), Query Frame = 3

Query:  150 RYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R +SNVFSMF + QIQEFKEAF +IDQN+DGFID +DL +   SLGK +  +  +E+MLK+A G INFTMFL LFGEK+ G+DPE+TILNAF  FDPE  G++  E L+ ++ +  ++++ E+V ++F  + I S G  +YR    +I HG+++E+
Sbjct:    1 RASSNVFSMFEQTQIQEFKEAFTLIDQNRDGFIDKEDLKDTYASLGKLNVKDNELEDMLKEASGPINFTMFLNLFGEKLHGTDPEDTILNAFKLFDPEAKGYIHTEELKHVLMTQANKFSAEEVRQMFQSSNIDSAGNLDYRSLCYIITHGEEQEE 156

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 201.06 bits (510), Expect = 3.615e-64
Identity = 102/173 (58.96%), Postives = 133/173 (76.88%), Query Frame = 3

Query:  102 TMASTKTRKKH--RPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            TM+S +T+ K   R R QR TSNVF+MF + QIQEFKEAFN++DQN+DG ID +DL +ML SLGK   E  +EEM+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD + TG ++E+H R+LMT+MGDR++D QVD+L   API   G+ NY EFT ++KHG +E+D
Sbjct:    4 TMSSRRTKGKSSFRKRPQRATSNVFAMFDKSQIQEFKEAFNIMDQNRDGLIDREDLQDMLASLGKTPTEEMLEEMMGEASGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEDGTGRINEDHFRELMTTMGDRFSDAQVDQLLAAAPIDQRGELNYLEFTHILKHGDREKD 176

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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 109.768 bits (273), Expect = 2.083e-29
Identity = 58/142 (40.85%), Postives = 90/142 (63.38%), Query Frame = 3

Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  G++S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF +M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 109.383 bits (272), Expect = 2.336e-29
Identity = 58/142 (40.85%), Postives = 90/142 (63.38%), Query Frame = 3

Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  G++S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF +M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.383 bits (272), Expect = 3.030e-29
Identity = 58/142 (40.85%), Postives = 90/142 (63.38%), Query Frame = 3

Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  G++S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF +M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 84.7297 bits (208), Expect = 9.015e-20
Identity = 53/155 (34.19%), Postives = 84/155 (54.19%), Query Frame = 3

Query:  144 TQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEML----KQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKH 593
             QR  S      SE Q QE KEAF++ D +  G ID  +L   + +LG +  +  I++M+    K   G INF  FL +  +KM+  D  E IL AF  FD ++TG +S ++L+ +   +G+  TDE++ E+   A    +G+ N  EF +++K 
Sbjct:   25 AQRKKSGPKPELSEEQKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMSEKDSREEILKAFRLFDDDETGKISFKNLKRVAKELGETLTDEELQEMIDEADRDGDGEINEEEFLRVMKK 179

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BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 87.0409 bits (214), Expect = 3.413e-21
Identity = 44/143 (30.77%), Postives = 80/143 (55.94%), Query Frame = 3

Query:  171 SMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMI 587
            S  +E QI EFKEAF + D++ +G I   +L  ++ SLG   +EA + +++ +        I F+ FL L   ++  +D E+ +L AF  FD    G +S   L+ ++TS+G++ TD +VD++       +G+ N ++F  ++
Sbjct:    3 SNLTEEQIAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMLREVSDGSGEINIQQFAALL 145

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: CDC31 (Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation [Source:SGD;Acc:S000005783])

HSP 1 Score: 64.3142 bits (155), Expect = 5.606e-13
Identity = 45/155 (29.03%), Postives = 77/155 (49.68%), Query Frame = 3

Query:  135 RPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG----KDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKM 584
            + R+   +  + S   E Q QE  EAF++ D N DGF+D+ +L   + +LG    K      I+E   +    + +  F  + GEK+   DP + I  AF  FD + TG +S ++LR +   +G+  TDE++  +     +  +G+ N  EF  +
Sbjct:    3 KNRSSLQSGPLNSELLEEQKQEIYEAFSLFDMNNDGFLDYHELKVAMKALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEKILKRDPLDEIKRAFQLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAI 157

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO44829 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUY1])

HSP 1 Score: 203.756 bits (517), Expect = 4.359e-65
Identity = 101/170 (59.41%), Postives = 132/170 (77.65%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISN-GKFNYREFTKMIKHGKKEED 611
            MAS KT+K  + R  R TSNVF+MF + QIQEFKEAFNMIDQN DGFID +DL +ML SLGKD  + Y+EEM+K++ G INFTMFLTLFGEK+ G+DPE+ I NAFACFD +  G + E+ L++ M SMGDR++DEQVD++   API   G  +Y +FT+++KHGKK+++
Sbjct:    1 MASRKTKKTTKKRAHRATSNVFAMFDQSQIQEFKEAFNMIDQNHDGFIDKEDLHDMLASLGKDPTDGYLEEMVKESPGPINFTMFLTLFGEKLNGTDPEDVIRNAFACFDEDGNGRIHEDLLKEAMMSMGDRFSDEQVDDILRDAPIDKEGFLDYSKFTRILKHGKKDDN 170

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO44767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUX1])

HSP 1 Score: 195.282 bits (495), Expect = 8.325e-62
Identity = 96/171 (56.14%), Postives = 132/171 (77.19%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVG-TINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPIS-NGKFNYREFTKMIKHGKKEED 611
            MAS KT+K  + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK  N+ ++++M+++A G TINFTMFLTLFGEK+ G+DPE+ I NAF CFD E  G + E+ L D++TS+GDR++++++DE+ H  P+   G  +Y  +T+M+K GKKE+D
Sbjct:    1 MASRKTKKTTKKRAQRATSNVFAMFDQTQIQEFKEAFNMIDQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLNGTDPEDVIKNAFGCFDEEGDGRIHEDKLCDMLTSVGDRFSEDEIDEMLHDCPVDKKGYIDYITYTQMLKRGKKEDD 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO25626 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVY3])

HSP 1 Score: 162.925 bits (411), Expect = 1.101e-49
Identity = 82/133 (61.65%), Postives = 105/133 (78.95%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVG-TINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDR 500
            MAS KT+K  + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK  N+ ++++M+++A G TINFTMFLTLFGEK+ G+DPE+ I NAF CFD E  G + E+ L D++TS+GDR
Sbjct:    1 MASRKTKKTTKKRAQRATSNVFAMFDQTQIQEFKEAFNMIDQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLNGTDPEDVIKNAFGCFDEEGDGRIHEDKLCDMLTSVGDR 133

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO42774 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0P5])

HSP 1 Score: 160.229 bits (404), Expect = 1.662e-48
Identity = 81/146 (55.48%), Postives = 105/146 (71.92%), Query Frame = 3

Query:  174 MFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPIS-NGKFNYREFTKMIKHGKKEE 608
            MF + QIQEFKEAFNMIDQN+DGFID +DL  +  SLGK  N+ Y+E+ML +A G INFTMFLTLFGEK++G+DPE+ I NAF  FD E  G + EE L+ L  SM DR T E+ +++    P +  G+  Y+EFT +IK+G KE+
Sbjct:    1 MFEQSQIQEFKEAFNMIDQNRDGFIDKNDLKAVFDSLGKLVNDEYVEDMLNEASGPINFTMFLTLFGEKISGTDPEDVIRNAFGSFDLEGKGSIDEEKLKRLCMSMSDRMTAEEWEDMMDECPTTKQGEILYKEFTDIIKNGPKED 146

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO35925 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK46])

HSP 1 Score: 117.087 bits (292), Expect = 1.157e-31
Identity = 62/158 (39.24%), Postives = 97/158 (61.39%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFH--GAPISNGKFNYREFTKMIKHGKKEE 608
            R  R  SN F+   + QI+ FKEAFN+IDQ+++G I  DDL   L+SLG+      I++M+ +   + NFT FL++FG +M  +DPE+ IL AF+C D    G +S + LR+L+T+MG R     V +LF   GA   +G  +Y +  +++K+G  ++
Sbjct:   18 RVNRVPSNFFTALDQSQIRLFKEAFNIIDQDRNGIITRDDLRGTLSSLGQKPTFEEIDDMM-EGKPSFNFTSFLSMFGLRMANTDPEDIILRAFSCLDDGGDGKLSAKMLRELLTTMGQRMKYSDVTQLFDDVGAD-QDGNLDYVKLVRLLKNGANDQ 173

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl9a (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:101167032])

HSP 1 Score: 221.476 bits (563), Expect = 3.045e-71
Identity = 105/172 (61.05%), Postives = 138/172 (80.23%), Query Frame = 3

Query:  102 TMASTKTR-KKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            TM+S + + K  + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAFACFD E +G + E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:   16 TMSSKRAKGKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKD 187

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl9a (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:101167032])

HSP 1 Score: 219.55 bits (558), Expect = 8.200e-71
Identity = 106/169 (62.72%), Postives = 135/169 (79.88%), Query Frame = 3

Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAFACFD E +G + E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKD 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl12.2 (myosin regulatory light chain 2, smooth muscle minor isoform [Source:NCBI gene;Acc:101165346])

HSP 1 Score: 217.624 bits (553), Expect = 5.231e-70
Identity = 104/171 (60.82%), Postives = 134/171 (78.36%), Query Frame = 3

Query:  105 MASTKTRKKH-RPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            M+S + + K+ + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G +NFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + EE LR+L+T+MGDR+TDE VDELF  API   G FNY  FT+++KHG K++D
Sbjct:    1 MSSKRAKGKNTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPVNFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGLIQEEFLRELLTTMGDRFTDEDVDELFREAPIDKKGNFNYVAFTRILKHGAKDKD 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: ENSORLT00000014284.2 (myosin regulatory light chain 2, smooth muscle minor isoform [Source:NCBI gene;Acc:101159052])

HSP 1 Score: 216.083 bits (549), Expect = 1.780e-69
Identity = 100/158 (63.29%), Postives = 130/158 (82.28%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGF+D +DL +ML SLGK+ N+ Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+T+E+VDELF  API     FNY EFT+++KHG +++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFVDKEDLHDMLASLGKNPNDDYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTEEEVDELFREAPIDKKSNFNYVEFTRILKHGARDKD 171

[Close]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: MYL2 (myosin regulatory light chain 2, ventricular/cardiac muscle isoform [Source:NCBI gene;Acc:101162219])

HSP 1 Score: 160.229 bits (404), Expect = 8.407e-48
Identity = 76/163 (46.63%), Postives = 119/163 (73.01%), Query Frame = 3

Query:  126 KKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEE 608
            KK + R++  +SNVFSMF + QIQEFKEAF ++DQN+DGFID +DL +   +LG+ +  +  I++MLK+A G INFT+FLT+FGEK+ G+DPEETILNAF  FDPE TG + ++ + +++T+  DR++ E+++++F    P   G  +Y+    +I HG++++
Sbjct:    4 KKAKKRSEGASSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRLNVKQEEIDDMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGTLKKDFVTEMLTTQADRFSAEEMEQMFVAFPPDVAGNLDYKNLVHIITHGEEKD 166

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000000175.1 (SMESG000000175.1)

HSP 1 Score: 363.999 bits (933), Expect = 9.803e-128
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 3

Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEEDLNNPNL 629
            MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDP+DTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEEDLNNPNL
Sbjct:    1 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPDDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEEDLNNPNL 175

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000066078.1 (SMESG000066078.1)

HSP 1 Score: 181.03 bits (458), Expect = 6.495e-56
Identity = 82/156 (52.56%), Postives = 118/156 (75.64%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEE 608
            R QR TSNVF MF + QIQEFKEAF MIDQN+DGFID +DL +M  SLG+  ++  ++EML ++ G +NFTMF+ LFGEK++GSDPEE++ NAF  FD ++ GF++EE+++DL+ +MGD +T E++ + +  AP++ GK +Y  F + IK GK +E
Sbjct:   12 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 167

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000026825.1 (SMESG000026825.1)

HSP 1 Score: 174.096 bits (440), Expect = 2.900e-53
Identity = 79/156 (50.64%), Postives = 113/156 (72.44%), Query Frame = 3

Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEE 608
            R QR TSNVF MF + QIQEFKEAF M+DQN+DGFID +DL +M  SLG+   +  + EML+++ G +NFTMF+ LFGEK+ G+DPEE + NAF  FD  +  ++ EE+++DL+ +MGD +T E++ + +  AP+S GK +Y  F + IK GK++E
Sbjct:   12 RAQRATSNVFGMFPQQQIQEFKEAFTMVDQNRDGFIDIEDLKDMYASLGRTPTDKELREMLEESPGPLNFTMFINLFGEKLNGTDPEEALRNAFKMFDSNNKEYLEEEYIKDLLQNMGDNFTPEEIRQTWKEAPLSGGKLDYEAFVRQIKRGKEDE 167

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000034095.1 (SMESG000034095.1)

HSP 1 Score: 109.383 bits (272), Expect = 9.382e-29
Identity = 59/142 (41.55%), Postives = 89/142 (62.68%), Query Frame = 3

Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 146

[Close]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000010769.1 (SMESG000010769.1)

HSP 1 Score: 109.383 bits (272), Expect = 9.382e-29
Identity = 59/142 (41.55%), Postives = 89/142 (62.68%), Query Frame = 3

Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 146

The following BLAST results are available for this feature:

BLAST of Myosin regulatory light chain vs. Ensembl Human
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
BLAST of Myosin regulatory light chain vs. Ensembl Fly
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
BLAST of Myosin regulatory light chain vs. TrEMBL
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
BLAST of Myosin regulatory light chain vs. Ensembl Yeast
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
BLAST of Myosin regulatory light chain vs. Planmine SMEST

BLAST of Myosin regulatory light chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
MYL12B	2.534e-69	63.92	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	2.534e-69	63.92	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	2.534e-69	63.92	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	2.534e-69	63.92	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]
MYL12B	2.534e-69	63.92	myosin light chain 12B [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mlc-4	1.257e-72	62.57	Myosin regulatory light chain [Source:UniProtKB/S...	[more]
mlc-1	1.117e-37	44.59	Myosin regulatory light chain 1 [Source:UniProtKB...	[more]
mlc-2	1.337e-37	44.59	Myosin regulatory light chain 2 [Source:UniProtKB...	[more]
cmd-1	8.948e-29	41.55	Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630...	[more]
cmd-1	2.640e-20	40.91	Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sqh	8.858e-68	57.56	gene:FBgn0003514 transcript:FBtr0339504	[more]
sqh	8.858e-68	57.56	gene:FBgn0003514 transcript:FBtr0339503	[more]
sqh	8.858e-68	57.56	gene:FBgn0003514 transcript:FBtr0070877	[more]
sqh	8.858e-68	57.56	gene:FBgn0003514 transcript:FBtr0339502	[more]
sqh	8.858e-68	57.56	gene:FBgn0003514 transcript:FBtr0345294	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl12.2	1.054e-71	63.91	myosin, light chain 12, genome duplicate 2 [Source...	[more]
myl12.2	1.054e-71	63.91	myosin, light chain 12, genome duplicate 2 [Source...	[more]
myl9a	2.182e-71	63.31	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
myl9a	2.182e-71	63.31	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
myl9a	2.182e-71	63.31	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9	5.203e-70	63.92	myosin light chain 9 [Source:NCBI gene;Acc:1001248...	[more]
myl12b	6.603e-68	63.29	myosin light chain 12B [Source:NCBI gene;Acc:44806...	[more]
ap5z1	3.056e-52	50.00	adaptor related protein complex 5 subunit zeta 1 [...	[more]
ap5z1	4.621e-52	48.82	adaptor related protein complex 5 subunit zeta 1 [...	[more]
tnrc6c	2.595e-46	49.68	trinucleotide repeat containing 6C [Source:Xenbase...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Gm49909	1.739e-69	63.92	predicted gene, 49909 [Source:MGI Symbol;Acc:MGI:6...	[more]
Myl12b	1.739e-69	63.92	myosin, light chain 12B, regulatory [Source:MGI Sy...	[more]
Myl12b	1.739e-69	63.92	myosin, light chain 12B, regulatory [Source:MGI Sy...	[more]
Myl9	6.812e-69	64.10	myosin, light polypeptide 9, regulatory [Source:MG...	[more]
Myl12a	1.833e-67	62.03	myosin, light chain 12A, regulatory, non-sarcomeri...	[more]

BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q09510\|MLRH_CAEEL	1.598e-71	62.57	Myosin regulatory light chain OS=Caenorhabditis el...	[more]
sp\|A4IF97\|ML12B_BOVIN	1.333e-69	62.35	Myosin regulatory light chain 12B OS=Bos taurus OX...	[more]
sp\|P24032\|MLRN_CHICK	6.704e-69	63.92	Myosin regulatory light chain 2, smooth muscle min...	[more]
sp\|P18666\|ML12B_RAT	1.178e-68	63.92	Myosin regulatory light chain 12B OS=Rattus norveg...	[more]
sp\|Q3THE2\|ML12B_MOUSE	1.217e-68	63.92	Myosin regulatory light chain 12B OS=Mus musculus ...	[more]

BLAST of Myosin regulatory light chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0X3Q2Q1	2.565e-83	69.82	Myosin regulatory light chain sqh OS=Schistocephal...	[more]
A0A5K3EJQ3	4.846e-83	71.84	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]
A0A087VX66	5.681e-83	71.59	Myosin regulatory light chain 2 smooth muscle OS=E...	[more]
A0A068X1P7	6.985e-83	71.59	Myosin regulatory light chain 2 smooth muscle OS=E...	[more]
W6USV8	1.249e-82	71.84	Myosin regulatory light chain sqh OS=Echinococcus ...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000048105.1	1.089e-70	63.31	pep primary_assembly:Astyanax_mexicanus-2.0:16:981...	[more]
myl9b	1.106e-70	62.72	myosin regulatory light polypeptide 9 [Source:NCBI...	[more]
ENSAMXT00000046001.1	1.433e-70	62.72	myosin regulatory light chain 2, smooth muscle min...	[more]
myl9a	2.052e-70	62.13	myosin regulatory light polypeptide 9-like [Source...	[more]
ENSAMXT00000051744.1	6.910e-49	50.64	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9a	3.615e-64	58.96	myosin, light chain 9a, regulatory [Source:ZFIN;Ac...	[more]
CALM3	2.083e-29	40.85	calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449]	[more]
CALML3	2.336e-29	40.85	calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145...	[more]
ENSPMAT00000006008.1	3.030e-29	40.85	pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-...	[more]
CETN1	9.015e-20	34.19	centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866]	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
CMD1	3.413e-21	30.77	Calmodulin; Ca2+ binding protein that regulates Ca...	[more]
CDC31	5.606e-13	29.03	Calcium-binding component of the spindle pole body...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO44829	4.359e-65	59.41	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO44767	8.325e-62	56.14	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO25626	1.101e-49	61.65	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8...	[more]
EDO42774	1.662e-48	55.48	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35925	1.157e-31	39.24	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
myl9a	3.045e-71	61.05	myosin regulatory light polypeptide 9 [Source:NCBI...	[more]
myl9a	8.200e-71	62.72	myosin regulatory light polypeptide 9 [Source:NCBI...	[more]
myl12.2	5.231e-70	60.82	myosin regulatory light chain 2, smooth muscle min...	[more]
ENSORLT00000014284.2	1.780e-69	63.29	myosin regulatory light chain 2, smooth muscle min...	[more]
MYL2	8.407e-48	46.63	myosin regulatory light chain 2, ventricular/cardi...	[more]

BLAST of Myosin regulatory light chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000000175.1	9.803e-128	99.43	SMESG000000175.1	[more]
SMESG000066078.1	6.495e-56	52.56	SMESG000066078.1	[more]
SMESG000026825.1	2.900e-53	50.64	SMESG000026825.1	[more]
SMESG000034095.1	9.382e-29	41.55	SMESG000034095.1	[more]
SMESG000010769.1	9.382e-29	41.55	SMESG000010769.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30019325 ID=SMED30019325|Name=Myosin regulatory light chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1094bp
CTTGTTTAAATCCATCAATACAATTTGTATGTATTATTCATATTTTAACA
ACTAATTTCCTATTTAAGTCAAAATTTCTTTTTAATAATATCATTTAATT
AACTATGGCATCTACAAAAACAAGAAAGAAGCATAGACCAAGAACCCAAA
GATATACTAGCAATGTATTTTCAATGTTCAGTGAACCTCAGATTCAAGAA
TTTAAAGAAGCATTCAATATGATTGACCAGAATAAAGACGGCTTTATTGA
TCACGATGATCTAGTGGAAATGCTTACTTCTCTTGGCAAAGATGCCAATG
AAGCATACATTGAAGAAATGCTCAAGCAAGCGGTGGGAACAATCAATTTT
ACTATGTTCTTAACATTATTTGGTGAAAAAATGACTGGGAGTGATCCGGA
AGAAACGATTTTGAATGCGTTTGCTTGCTTTGATCCAGAGGACACGGGAT
TCGTCTCCGAAGAACATCTTCGAGATTTAATGACTTCAATGGGGGATAGA
TGGACAGACGAACAAGTCGATGAATTATTCCACGGAGCACCGATATCAAA
TGGTAAATTCAACTATAGAGAATTTACAAAAATGATTAAGCATGGAAAGA
AAGAAGAAGATTTAAATAATCCGAATCTTTAAGTGACTCAATCGTCTAAT
TGAATTTGAATAAAAGTTATTAATTTATAGTTTTTTTAAAATTAAATGTT
ATATTTCTGTTTTCATAATATTTTATTTTCACTTGGAATTTAGTTGTTTG
CAAATTTGAAATAGGATGTTAAGATGTTTATCAAATTATTATGAATCGAT
TTATTTATGTGCATATGGTATCTCGTGAATTATTAGCTTTCATTTCATGC
ATAATTGATTGATGTGGAGGTAGCTATTGAACAAATCTGTGTGTTCTTGT
TATTTGTGTGCTATTGTAGCAAAAACGGTGTCTTGTTCACATGCGAACAT
TTATTAAGACGTATGCATTGTTTACGTTTCATAGGTTAGTTTGTTTTGAT
AAAGGTGACAAATTTTATTTTCAAATTTTTTTGATAAACTTGTCCGTTTT
TGTAATTTTTTTGCAAGAGATATTGATTTAAGTCTTTAAAAAAA

protein sequence of SMED30019325-orf-1

>SMED30019325-orf-1 ID=SMED30019325-orf-1|Name=SMED30019325-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=176bp
MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDH
DDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEE
TILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNG
KFNYREFTKMIKHGKKEEDLNNPNL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000026	gut
PLANA:0000034	epidermis
PLANA:0000035	Category 3 cell
PLANA:0000070	intestinal phagocyte
PLANA:0002032	epidermal cell
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding
GO:0046872	metal ion binding

Vocabulary: **biological process**
Term	Definition
GO:0000281	mitotic cytokinesis
GO:0002119	nematode larval development
GO:0007110	meiosis I cytokinesis
GO:0008105	asymmetric protein localization
GO:0010172	embryonic body morphogenesis
GO:0030010	establishment of cell polarity

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm
GO:0005856	cytoskeleton
GO:0016459	myosin complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 101..129 e-value: 0.15 score: 21.2 coord: 32..60 e-value: 2.5E-7 score: 40.4
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 32..60 e-value: 3.2E-8 score: 32.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 97..132 score: 12.198
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 28..63 score: 15.88
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 32..89 e-value: 4.51887E-8 score: 45.6165
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 105..162 e-value: 6.74931E-7 score: 42.5349
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 95..162 e-value: 2.5E-18 score: 68.1
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 14..94 e-value: 3.6E-23 score: 83.4
None	No IPR available	PANTHER	PTHR23049:SF66	MYOSIN REGULATORY LIGHT POLYPEPTIDE 9	coord: 4..169
None	No IPR available	PANTHER	PTHR23049	MYOSIN REGULATORY LIGHT CHAIN 2	coord: 4..169
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 41..53
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 25..164