Myosin regulatory light chain

Overview
NameMyosin regulatory light chain
Smed IDSMED30019325
Length (bp)1094
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Myosin regulatory light chain (SMED30019325) t-SNE clustered cells

Violin plots show distribution of expression levels for Myosin regulatory light chain (SMED30019325) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myosin regulatory light chain (SMED30019325) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myosin regulatory light chain (SMED30019325) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019325

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30019325SMESG000036544.1 Contig3175GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30019325SMESG000036544.1 Contig3175GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30019325SMESG000068156.1 SMESG000064685.1 SMESG000043474.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000058575.1 SMESG000053533.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig246GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30019325SMESG000068156.1 SMESG000064685.1 SMESG000043474.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000058575.1 SMESG000053533.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig246GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30019325SMESG000068156.1 SMESG000064685.1 SMESG000043474.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000058575.1 SMESG000053533.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig246GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30019325SMESG000000175.1 Contig246GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30019325SMESG000000175.1 Contig246GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30019325SMESG000000175.1 Contig246GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30019325SMESG000000175.1 Contig3175GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30019325SMESG000000175.1 Contig3175GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30019325SMESG000000175.1 Contig2068GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30019325SMESG000000175.1 Contig2068GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30019325SMESG000000175.1 Contig2068GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
protonephridiaSMED30019325SMESG000000175.1 dd_Smed_v4_383_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019325SMESG000000175.1 dd_Smed_v4_383_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30019325SMESG000000175.1 dd_Smed_v4_383_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019325SMESG000000175.1 dd_Smed_v6_383_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30019325SMESG000000175.1 dd_Smed_v6_383_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019325SMESG000000175.1 dd_Smed_v6_383_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 216.468 bits (550), Expect = 2.534e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 216.468 bits (550), Expect = 2.534e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 216.468 bits (550), Expect = 2.534e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 216.468 bits (550), Expect = 2.534e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Human
Match: MYL12B (myosin light chain 12B [Source:HGNC Symbol;Acc:HGNC:29827])

HSP 1 Score: 216.468 bits (550), Expect = 2.534e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-4 (Myosin regulatory light chain [Source:UniProtKB/Swiss-Prot;Acc:Q09510])

HSP 1 Score: 223.787 bits (569), Expect = 1.257e-72
Identity = 107/171 (62.57%), Postives = 138/171 (80.70%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGT--INFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            MAS KT  + R R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK+  E +I+ M+ +A G   INFTMFLTLFGEK+TG+DPEE I NAF CFD +++G ++EEHLR+L+T+MG+R+++EQVDELF  API  G+F+Y EFT+M+KHG K++D
Sbjct:    1 MASRKTVNR-RQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASLGKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLNEEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKDKD 170          
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-1 (Myosin regulatory light chain 1 [Source:UniProtKB/Swiss-Prot;Acc:P19625])

HSP 1 Score: 133.65 bits (335), Expect = 1.117e-37
Identity = 66/148 (44.59%), Postives = 100/148 (67.57%), Query Frame = 3
Query:  171 SMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHL-RDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEE 608
            + F +  IQEFKEAF ++DQNKDG ID  DL ++  S+G+ A ++ I+ M+K+A G INFT+FLTLFGE++TG+DPE TI+ AFA FD +D G + E+ L + L    G+   +++V  ++ G  PI  G+ +Y+ F  +I  G ++E
Sbjct:   19 AQFDQKTIQEFKEAFGIMDQNKDGIIDKSDLKDLYASMGQIAPDSQIDAMIKEASGPINFTVFLTLFGERLTGTDPEATIIGAFAMFDKKDCGKIKEDDLIKILQNKRGEPLDEDEVKAMYKGKPPIEGGEVDYKAFAHLITTGAQDE 166          
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: mlc-2 (Myosin regulatory light chain 2 [Source:UniProtKB/Swiss-Prot;Acc:P19626])

HSP 1 Score: 133.265 bits (334), Expect = 1.337e-37
Identity = 66/148 (44.59%), Postives = 100/148 (67.57%), Query Frame = 3
Query:  171 SMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHL-RDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEE 608
            + F +  IQEFKEAF ++DQNKDG ID  DL ++  S+G+ A ++ I+ M+K+A G INFT+FLTLFGE++TG+DPE TI+ AFA FD +D G + E+ L + L    G+   +++V  ++ G  PI  G+ +Y+ F  +I  G ++E
Sbjct:   19 AQFDQKTIQEFKEAFGIMDQNKDGIIDKSDLKDLYASMGQIAPDSQIDAMIKEASGPINFTVFLTLFGERLTGTDPEATIVGAFAMFDKKDCGKIKEDDLIKILQNKRGEPLDEDEVKAMYKGKPPIEGGEVDYKAFAHLITTGAQDE 166          
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 109.383 bits (272), Expect = 8.948e-29
Identity = 59/142 (41.55%), Postives = 89/142 (62.68%), Query Frame = 3
Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 146          
BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 85.5001 bits (210), Expect = 2.640e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 3
Query:  273 LTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
            + SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    1 MRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 110          
BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339504)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3
Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339503)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3
Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0070877)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3
Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0339502)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3
Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Fly
Match: sqh (gene:FBgn0003514 transcript:FBtr0345294)

HSP 1 Score: 211.846 bits (538), Expect = 8.858e-68
Identity = 99/172 (57.56%), Postives = 133/172 (77.33%), Query Frame = 3
Query:  105 MASTKT---RKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M+S KT   R   + R QR TSNVF+MF + QI EFKEAFNMIDQN+DGF++ +DL +ML SLGK+  + Y++ M+ +A G INFTMFLTLFGE++ G+DPE+ I NAF CFD E+ G + E+ LR+L+T+MGDR+TDE VDE++  API NG F+Y EFT+++KHG K++D
Sbjct:    1 MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl12.2 (myosin, light chain 12, genome duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9028])

HSP 1 Score: 221.861 bits (564), Expect = 1.054e-71
Identity = 108/169 (63.91%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIKNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl12.2 (myosin, light chain 12, genome duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-9028])

HSP 1 Score: 221.861 bits (564), Expect = 1.054e-71
Identity = 108/169 (63.91%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIKNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 221.476 bits (563), Expect = 2.182e-71
Identity = 107/169 (63.31%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + TGF+ E+HLRDL+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 221.476 bits (563), Expect = 2.182e-71
Identity = 107/169 (63.31%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + TGF+ E+HLRDL+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 221.476 bits (563), Expect = 2.182e-71
Identity = 107/169 (63.31%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + TGF+ E+HLRDL+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDATGFIHEDHLRDLLTTMGDRFTDEEVDELFREAPIDKKGNFNYVEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl9 (myosin light chain 9 [Source:NCBI gene;Acc:100124811])

HSP 1 Score: 218.009 bits (554), Expect = 5.203e-70
Identity = 101/158 (63.92%), Postives = 131/158 (82.91%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+GK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E +GF++E+HLR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG KE+D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFINEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKEKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: myl12b (myosin light chain 12B [Source:NCBI gene;Acc:448060])

HSP 1 Score: 212.616 bits (540), Expect = 6.603e-68
Identity = 100/158 (63.29%), Postives = 127/158 (80.38%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E  G + E+ LR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:   15 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGIGSIQEDTLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYNEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: ap5z1 (adaptor related protein complex 5 subunit zeta 1 [Source:Xenbase;Acc:XB-GENE-6034870])

HSP 1 Score: 172.17 bits (435), Expect = 3.056e-52
Identity = 82/164 (50.00%), Postives = 117/164 (71.34%), Query Frame = 3
Query:  126 KKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEED 611
            KK + R +   SNVFSMF + QIQEFKEAF ++DQN+DGFID +DL +   +LG+ +     +EEMLK+A G INFT+FLT+FGEK+ G+DPEETILNAF  FDPE TG +  E++R+++ +  +R+T E+VD++F    P   G  NY+    +I HG+++E+
Sbjct:    4 KKAKKRAEGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRLNVKNEELEEMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGLLKSEYIREMLMTQAERFTSEEVDQMFTAFPPDVTGNLNYKNLVHIITHGEEKEE 167          
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: ap5z1 (adaptor related protein complex 5 subunit zeta 1 [Source:Xenbase;Acc:XB-GENE-6034870])

HSP 1 Score: 174.481 bits (441), Expect = 4.621e-52
Identity = 83/170 (48.82%), Postives = 120/170 (70.59%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEED 611
            A+  + KK + R +   SNVFSMF + QIQEFKEAF ++DQN+DGFID +DL +   +LG+ +     +EEMLK+A G INFT+FLT+FGEK+ G+DPEETILNAF  FDPE TG +  E++R+++ +  +R+T E+VD++F    P   G  NY+    +I HG+++E+
Sbjct:   76 AANTSPKKAKKRAEGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKEDLRDTFAALGRLNVKNEELEEMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGLLKSEYIREMLMTQAERFTSEEVDQMFTAFPPDVTGNLNYKNLVHIITHGEEKEE 245          
BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Match: tnrc6c (trinucleotide repeat containing 6C [Source:Xenbase;Acc:XB-GENE-6044444])

HSP 1 Score: 157.147 bits (396), Expect = 2.595e-46
Identity = 78/157 (49.68%), Postives = 109/157 (69.43%), Query Frame = 3
Query:  147 QRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPIS-NGKFNYREFTKMIKHGKKEED 611
            QR +SNVFSMF + QIQEFKEAF+ IDQN+DG I   DL E    LGK + NE  ++EMLK+  G INFT+FL+LFGEK+ G+DPE++IL AF   DP  TG ++++ L+ L+ +  D++T E+VD++F   PI   G  +Y+    +I HG ++ED
Sbjct:   16 QRGSSNVFSMFEQSQIQEFKEAFSCIDQNRDGIITKSDLKETYMQLGKMNVNEDELDEMLKEGKGPINFTVFLSLFGEKLNGTDPEDSILAAFKILDPNATGNINKDELKQLLMTQADKFTAEEVDQMFAVTPIDVAGNIDYKSLCYIITHGDEKED 172          
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Gm49909 (predicted gene, 49909 [Source:MGI Symbol;Acc:MGI:6270609])

HSP 1 Score: 216.468 bits (550), Expect = 1.739e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12b (myosin, light chain 12B, regulatory [Source:MGI Symbol;Acc:MGI:107494])

HSP 1 Score: 216.468 bits (550), Expect = 1.739e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12b (myosin, light chain 12B, regulatory [Source:MGI Symbol;Acc:MGI:107494])

HSP 1 Score: 216.468 bits (550), Expect = 1.739e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl9 (myosin, light polypeptide 9, regulatory [Source:MGI Symbol;Acc:MGI:2138915])

HSP 1 Score: 214.927 bits (546), Expect = 6.812e-69
Identity = 100/156 (64.10%), Postives = 129/156 (82.69%), Query Frame = 3
Query:  147 QRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E +GF+ E+HLR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG K++D
Sbjct:   16 QRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Match: Myl12a (myosin, light chain 12A, regulatory, non-sarcomeric [Source:MGI Symbol;Acc:MGI:1914518])

HSP 1 Score: 211.46 bits (537), Expect = 1.833e-67
Identity = 98/158 (62.03%), Postives = 128/158 (81.01%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+GK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E  G + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEAIGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|Q09510|MLRH_CAEEL (Myosin regulatory light chain OS=Caenorhabditis elegans OX=6239 GN=mlc-4 PE=1 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 1.598e-71
Identity = 107/171 (62.57%), Postives = 138/171 (80.70%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGT--INFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            MAS KT  + R R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK+  E +I+ M+ +A G   INFTMFLTLFGEK+TG+DPEE I NAF CFD +++G ++EEHLR+L+T+MG+R+++EQVDELF  API  G+F+Y EFT+M+KHG K++D
Sbjct:    1 MASRKTVNR-RQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASLGKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLNEEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKDKD 170          
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|A4IF97|ML12B_BOVIN (Myosin regulatory light chain 12B OS=Bos taurus OX=9913 GN=MYL12B PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 1.333e-69
Identity = 106/170 (62.35%), Postives = 137/170 (80.59%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            M+S K + K + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:    1 MSSKKAKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 170          
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P24032|MLRN_CHICK (Myosin regulatory light chain 2, smooth muscle minor isoform OS=Gallus gallus OX=9031 PE=2 SV=2)

HSP 1 Score: 217.238 bits (552), Expect = 6.704e-69
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|P18666|ML12B_RAT (Myosin regulatory light chain 12B OS=Rattus norvegicus OX=10116 GN=Myl12b PE=1 SV=3)

HSP 1 Score: 216.468 bits (550), Expect = 1.178e-68
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGRINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Match: sp|Q3THE2|ML12B_MOUSE (Myosin regulatory light chain 12B OS=Mus musculus OX=10090 GN=Myl12b PE=1 SV=2)

HSP 1 Score: 216.468 bits (550), Expect = 1.217e-68
Identity = 101/158 (63.92%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + E++LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A0X3Q2Q1 (Myosin regulatory light chain sqh OS=Schistocephalus solidus OX=70667 GN=SQH PE=4 SV=1)

HSP 1 Score: 260.381 bits (664), Expect = 2.565e-83
Identity = 118/169 (69.82%), Postives = 148/169 (87.57%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 611
            M++TK++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +EAY++EMLKQA G INFTMFLTLFGEKM G DPE++ILNAFACFDP +TG+++EE LR+LMT+MGDRWTD++VDELFHGAPI +G+FNYREFTKMI HG+ +E+
Sbjct:    1 MSATKSKKRGRARTQRYTSNVFSMFTEAQINEFKEAFSIIDGSKDGIIDQSDLEEVFRSMGKNPSEAYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEDSILNAFACFDPNNTGYINEERLRELMTTMGDRWTDDKVDELFHGAPIKDGEFNYREFTKMIMHGQDDEE 169          
BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A5K3EJQ3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 4.846e-83
Identity = 125/174 (71.84%), Postives = 148/174 (85.06%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNP 623
            MASTKT+K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+  EAY+EEMLKQA G INFTMFLTLFGEKM G DPE+TILNAFA FDP + G ++EE LR+LMT+MGDRWTDE+VDELFHGAPI +G+FNYREFTKMI HG++ +ED N+P
Sbjct:    1 MASTKTKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPTEAYLEEMLKQAPGCINFTMFLTLFGEKMMGCDPEDTILNAFAQFDPNNKGVINEERLRELMTTMGDRWTDEKVDELFHGAPIQDGEFNYREFTKMIIHGQQDDEDQNHP 174          
BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A087VX66 (Myosin regulatory light chain 2 smooth muscle OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000041600 PE=4 SV=1)

HSP 1 Score: 260.381 bits (664), Expect = 5.681e-83
Identity = 126/176 (71.59%), Postives = 148/176 (84.09%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNPNL 629
            MA  K++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +E Y++EMLKQA G INFTMFLTLFGEKM G DPEETILNAFA FDP +TG +SEE LRDLMT+MGDRWTDE+VDELFHGAPI NG FNYREFTKMI HG++ +ED N+P+L
Sbjct:    1 MAFAKSKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPSEEYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEETILNAFAQFDPNNTGVISEERLRDLMTTMGDRWTDEKVDELFHGAPIKNGDFNYREFTKMIMHGQQDDEDQNHPSL 176          
BLAST of Myosin regulatory light chain vs. TrEMBL
Match: A0A068X1P7 (Myosin regulatory light chain 2 smooth muscle OS=Echinococcus granulosus OX=6210 GN=EgrG_000041600 PE=4 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 6.985e-83
Identity = 126/176 (71.59%), Postives = 148/176 (84.09%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNPNL 629
            MA  K++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +E Y++EMLKQA G INFTMFLTLFGEKM G DPEETILNAFA FDP +TG +SEE LRDLMT+MGDRWTDE+VDELFHGAPI NG FNYREFTKMI HG++ +ED N+P+L
Sbjct:    1 MAFAKSKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPSEEYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEETILNAFAQFDPNNTGVISEERLRDLMTTMGDRWTDEKVDELFHGAPIKNGDFNYREFTKMIMHGQQDDEDQNHPSL 176          
BLAST of Myosin regulatory light chain vs. TrEMBL
Match: W6USV8 (Myosin regulatory light chain sqh OS=Echinococcus granulosus OX=6210 GN=EGR_00670 PE=4 SV=1)

HSP 1 Score: 258.455 bits (659), Expect = 1.249e-82
Identity = 125/174 (71.84%), Postives = 146/174 (83.91%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKK-EEDLNNP 623
            MA  K++K+ R RTQRYTSNVFSMF+E QI EFKEAF++ID +KDG ID  DL E+  S+GK+ +E Y++EMLKQA G INFTMFLTLFGEKM G DPEETILNAFA FDP +TG +SEE LRDLMT+MGDRWTDE+VDELFHGAPI NG FNYREFTKMI HG++ +ED N+P
Sbjct:    1 MAFAKSKKRVRSRTQRYTSNVFSMFTEAQINEFKEAFSLIDGSKDGIIDQGDLEEVFRSMGKNPSEEYLDEMLKQAPGCINFTMFLTLFGEKMMGCDPEETILNAFAQFDPNNTGVISEERLRDLMTTMGDRWTDEKVDELFHGAPIKNGDFNYREFTKMIMHGQQDDEDQNHP 174          
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000048105.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:9819022:9829334:-1 gene:ENSAMXG00000037886.1 transcript:ENSAMXT00000048105.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 219.935 bits (559), Expect = 1.089e-70
Identity = 107/169 (63.31%), Postives = 134/169 (79.29%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API   G FNY  FT+++KHG K++D
Sbjct:   24 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVAFTRILKHGAKDKD 189          
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: myl9b (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:103045271])

HSP 1 Score: 219.164 bits (557), Expect = 1.106e-70
Identity = 106/169 (62.72%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAFACFD E +G + E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDDYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000046001.1 (myosin regulatory light chain 2, smooth muscle minor isoform-like [Source:NCBI gene;Acc:103039566])

HSP 1 Score: 218.779 bits (556), Expect = 1.433e-70
Identity = 106/169 (62.72%), Postives = 134/169 (79.29%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGF+D +DL +ML SLGK+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+TDE+VDELF  API     FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFVDKEDLHDMLASLGKNPTDDYLEAMMTEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTDEEVDELFREAPIDKKSNFNYVEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: myl9a (myosin regulatory light polypeptide 9-like [Source:NCBI gene;Acc:103022187])

HSP 1 Score: 218.779 bits (556), Expect = 2.052e-70
Identity = 105/169 (62.13%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAF CFD + +GF+ E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    7 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFTCFDEDGSGFIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYTEFTRILKHGAKDKD 172          
BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Match: ENSAMXT00000051744.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000067.1:2135412:2141173:-1 gene:ENSAMXG00000034506.1 transcript:ENSAMXT00000051744.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 162.54 bits (410), Expect = 6.910e-49
Identity = 79/156 (50.64%), Postives = 113/156 (72.44%), Query Frame = 3
Query:  150 RYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R +SNVFSMF + QIQEFKEAF +IDQN+DGFID +DL +   SLGK +  +  +E+MLK+A G INFTMFL LFGEK+ G+DPE+TILNAF  FDPE  G++  E L+ ++ +  ++++ E+V ++F  + I S G  +YR    +I HG+++E+
Sbjct:    1 RASSNVFSMFEQTQIQEFKEAFTLIDQNRDGFIDKEDLKDTYASLGKLNVKDNELEDMLKEASGPINFTMFLNLFGEKLHGTDPEDTILNAFKLFDPEAKGYIHTEELKHVLMTQANKFSAEEVRQMFQSSNIDSAGNLDYRSLCYIITHGEEQEE 156          
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: myl9a (myosin, light chain 9a, regulatory [Source:ZFIN;Acc:ZDB-GENE-041010-120])

HSP 1 Score: 201.06 bits (510), Expect = 3.615e-64
Identity = 102/173 (58.96%), Postives = 133/173 (76.88%), Query Frame = 3
Query:  102 TMASTKTRKKH--RPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            TM+S +T+ K   R R QR TSNVF+MF + QIQEFKEAFN++DQN+DG ID +DL +ML SLGK   E  +EEM+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD + TG ++E+H R+LMT+MGDR++D QVD+L   API   G+ NY EFT ++KHG +E+D
Sbjct:    4 TMSSRRTKGKSSFRKRPQRATSNVFAMFDKSQIQEFKEAFNIMDQNRDGLIDREDLQDMLASLGKTPTEEMLEEMMGEASGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEDGTGRINEDHFRELMTTMGDRFSDAQVDQLLAAAPIDQRGELNYLEFTHILKHGDREKD 176          
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 109.768 bits (273), Expect = 2.083e-29
Identity = 58/142 (40.85%), Postives = 90/142 (63.38%), Query Frame = 3
Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  G++S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF +M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146          
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 109.383 bits (272), Expect = 2.336e-29
Identity = 58/142 (40.85%), Postives = 90/142 (63.38%), Query Frame = 3
Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  G++S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF +M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146          
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.383 bits (272), Expect = 3.030e-29
Identity = 58/142 (40.85%), Postives = 90/142 (63.38%), Query Frame = 3
Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  G++S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF +M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMM 146          
BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 84.7297 bits (208), Expect = 9.015e-20
Identity = 53/155 (34.19%), Postives = 84/155 (54.19%), Query Frame = 3
Query:  144 TQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEML----KQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKH 593
             QR  S      SE Q QE KEAF++ D +  G ID  +L   + +LG +  +  I++M+    K   G INF  FL +  +KM+  D  E IL AF  FD ++TG +S ++L+ +   +G+  TDE++ E+   A    +G+ N  EF +++K 
Sbjct:   25 AQRKKSGPKPELSEEQKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMSEKDSREEILKAFRLFDDDETGKISFKNLKRVAKELGETLTDEELQEMIDEADRDGDGEINEEEFLRVMKK 179          
BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 87.0409 bits (214), Expect = 3.413e-21
Identity = 44/143 (30.77%), Postives = 80/143 (55.94%), Query Frame = 3
Query:  171 SMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMI 587
            S  +E QI EFKEAF + D++ +G I   +L  ++ SLG   +EA + +++ +        I F+ FL L   ++  +D E+ +L AF  FD    G +S   L+ ++TS+G++ TD +VD++       +G+ N ++F  ++
Sbjct:    3 SNLTEEQIAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMLREVSDGSGEINIQQFAALL 145          
BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Match: CDC31 (Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation [Source:SGD;Acc:S000005783])

HSP 1 Score: 64.3142 bits (155), Expect = 5.606e-13
Identity = 45/155 (29.03%), Postives = 77/155 (49.68%), Query Frame = 3
Query:  135 RPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG----KDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKM 584
            + R+   +  + S   E Q QE  EAF++ D N DGF+D+ +L   + +LG    K      I+E   +    + +  F  + GEK+   DP + I  AF  FD + TG +S ++LR +   +G+  TDE++  +     +  +G+ N  EF  +
Sbjct:    3 KNRSSLQSGPLNSELLEEQKQEIYEAFSLFDMNNDGFLDYHELKVAMKALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEKILKRDPLDEIKRAFQLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAI 157          
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO44829 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUY1])

HSP 1 Score: 203.756 bits (517), Expect = 4.359e-65
Identity = 101/170 (59.41%), Postives = 132/170 (77.65%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISN-GKFNYREFTKMIKHGKKEED 611
            MAS KT+K  + R  R TSNVF+MF + QIQEFKEAFNMIDQN DGFID +DL +ML SLGKD  + Y+EEM+K++ G INFTMFLTLFGEK+ G+DPE+ I NAFACFD +  G + E+ L++ M SMGDR++DEQVD++   API   G  +Y +FT+++KHGKK+++
Sbjct:    1 MASRKTKKTTKKRAHRATSNVFAMFDQSQIQEFKEAFNMIDQNHDGFIDKEDLHDMLASLGKDPTDGYLEEMVKESPGPINFTMFLTLFGEKLNGTDPEDVIRNAFACFDEDGNGRIHEDLLKEAMMSMGDRFSDEQVDDILRDAPIDKEGFLDYSKFTRILKHGKKDDN 170          
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO44767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUX1])

HSP 1 Score: 195.282 bits (495), Expect = 8.325e-62
Identity = 96/171 (56.14%), Postives = 132/171 (77.19%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVG-TINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPIS-NGKFNYREFTKMIKHGKKEED 611
            MAS KT+K  + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK  N+ ++++M+++A G TINFTMFLTLFGEK+ G+DPE+ I NAF CFD E  G + E+ L D++TS+GDR++++++DE+ H  P+   G  +Y  +T+M+K GKKE+D
Sbjct:    1 MASRKTKKTTKKRAQRATSNVFAMFDQTQIQEFKEAFNMIDQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLNGTDPEDVIKNAFGCFDEEGDGRIHEDKLCDMLTSVGDRFSEDEIDEMLHDCPVDKKGYIDYITYTQMLKRGKKEDD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO25626 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVY3])

HSP 1 Score: 162.925 bits (411), Expect = 1.101e-49
Identity = 82/133 (61.65%), Postives = 105/133 (78.95%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVG-TINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDR 500
            MAS KT+K  + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SLGK  N+ ++++M+++A G TINFTMFLTLFGEK+ G+DPE+ I NAF CFD E  G + E+ L D++TS+GDR
Sbjct:    1 MASRKTKKTTKKRAQRATSNVFAMFDQTQIQEFKEAFNMIDQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLNGTDPEDVIKNAFGCFDEEGDGRIHEDKLCDMLTSVGDR 133          
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO42774 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0P5])

HSP 1 Score: 160.229 bits (404), Expect = 1.662e-48
Identity = 81/146 (55.48%), Postives = 105/146 (71.92%), Query Frame = 3
Query:  174 MFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPIS-NGKFNYREFTKMIKHGKKEE 608
            MF + QIQEFKEAFNMIDQN+DGFID +DL  +  SLGK  N+ Y+E+ML +A G INFTMFLTLFGEK++G+DPE+ I NAF  FD E  G + EE L+ L  SM DR T E+ +++    P +  G+  Y+EFT +IK+G KE+
Sbjct:    1 MFEQSQIQEFKEAFNMIDQNRDGFIDKNDLKAVFDSLGKLVNDEYVEDMLNEASGPINFTMFLTLFGEKISGTDPEDVIRNAFGSFDLEGKGSIDEEKLKRLCMSMSDRMTAEEWEDMMDECPTTKQGEILYKEFTDIIKNGPKED 146          
BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Match: EDO35925 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK46])

HSP 1 Score: 117.087 bits (292), Expect = 1.157e-31
Identity = 62/158 (39.24%), Postives = 97/158 (61.39%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFH--GAPISNGKFNYREFTKMIKHGKKEE 608
            R  R  SN F+   + QI+ FKEAFN+IDQ+++G I  DDL   L+SLG+      I++M+ +   + NFT FL++FG +M  +DPE+ IL AF+C D    G +S + LR+L+T+MG R     V +LF   GA   +G  +Y +  +++K+G  ++
Sbjct:   18 RVNRVPSNFFTALDQSQIRLFKEAFNIIDQDRNGIITRDDLRGTLSSLGQKPTFEEIDDMM-EGKPSFNFTSFLSMFGLRMANTDPEDIILRAFSCLDDGGDGKLSAKMLRELLTTMGQRMKYSDVTQLFDDVGAD-QDGNLDYVKLVRLLKNGANDQ 173          
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl9a (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:101167032])

HSP 1 Score: 221.476 bits (563), Expect = 3.045e-71
Identity = 105/172 (61.05%), Postives = 138/172 (80.23%), Query Frame = 3
Query:  102 TMASTKTR-KKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            TM+S + + K  + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAFACFD E +G + E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:   16 TMSSKRAKGKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKD 187          
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl9a (myosin regulatory light polypeptide 9 [Source:NCBI gene;Acc:101167032])

HSP 1 Score: 219.55 bits (558), Expect = 8.200e-71
Identity = 106/169 (62.72%), Postives = 135/169 (79.88%), Query Frame = 3
Query:  108 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+ ++ Y+E M+ +A G INFTMFLT+FGE++ G+DPE+ I NAFACFD E +G + E+HLR+L+T+MGDR+TDE+VDELF  API   G FNY EFT+++KHG K++D
Sbjct:    6 AKGKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPSDEYLEGMMSEAPGPINFTMFLTMFGERLNGTDPEDVIRNAFACFDEEGSGVIHEDHLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYAEFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: myl12.2 (myosin regulatory light chain 2, smooth muscle minor isoform [Source:NCBI gene;Acc:101165346])

HSP 1 Score: 217.624 bits (553), Expect = 5.231e-70
Identity = 104/171 (60.82%), Postives = 134/171 (78.36%), Query Frame = 3
Query:  105 MASTKTRKKH-RPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            M+S + + K+ + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLGK+  + Y+E M+ +A G +NFTMFLT+FGEK+ G+DPE+ I NAFACFD E TG + EE LR+L+T+MGDR+TDE VDELF  API   G FNY  FT+++KHG K++D
Sbjct:    1 MSSKRAKGKNTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEAMMNEAPGPVNFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGLIQEEFLRELLTTMGDRFTDEDVDELFREAPIDKKGNFNYVAFTRILKHGAKDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: ENSORLT00000014284.2 (myosin regulatory light chain 2, smooth muscle minor isoform [Source:NCBI gene;Acc:101159052])

HSP 1 Score: 216.083 bits (549), Expect = 1.780e-69
Identity = 100/158 (63.29%), Postives = 130/158 (82.28%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 611
            R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGF+D +DL +ML SLGK+ N+ Y+E M+ +A G INFTMFLT+FGEK+ G+DPE+ I NAFACFD E TGF+ E++LR+L+T+MGDR+T+E+VDELF  API     FNY EFT+++KHG +++D
Sbjct:   14 RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFVDKEDLHDMLASLGKNPNDDYLEAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGFIQEDYLRELLTTMGDRFTEEEVDELFREAPIDKKSNFNYVEFTRILKHGARDKD 171          
BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Match: MYL2 (myosin regulatory light chain 2, ventricular/cardiac muscle isoform [Source:NCBI gene;Acc:101162219])

HSP 1 Score: 160.229 bits (404), Expect = 8.407e-48
Identity = 76/163 (46.63%), Postives = 119/163 (73.01%), Query Frame = 3
Query:  126 KKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGK-DANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHG-APISNGKFNYREFTKMIKHGKKEE 608
            KK + R++  +SNVFSMF + QIQEFKEAF ++DQN+DGFID +DL +   +LG+ +  +  I++MLK+A G INFT+FLT+FGEK+ G+DPEETILNAF  FDPE TG + ++ + +++T+  DR++ E+++++F    P   G  +Y+    +I HG++++
Sbjct:    4 KKAKKRSEGASSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRLNVKQEEIDDMLKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGTGTLKKDFVTEMLTTQADRFSAEEMEQMFVAFPPDVAGNLDYKNLVHIITHGEEKD 166          
BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000000175.1 (SMESG000000175.1)

HSP 1 Score: 363.999 bits (933), Expect = 9.803e-128
Identity = 174/175 (99.43%), Postives = 175/175 (100.00%), Query Frame = 3
Query:  105 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEEDLNNPNL 629
            MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDP+DTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEEDLNNPNL
Sbjct:    1 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPDDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEEDLNNPNL 175          
BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000066078.1 (SMESG000066078.1)

HSP 1 Score: 181.03 bits (458), Expect = 6.495e-56
Identity = 82/156 (52.56%), Postives = 118/156 (75.64%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEE 608
            R QR TSNVF MF + QIQEFKEAF MIDQN+DGFID +DL +M  SLG+  ++  ++EML ++ G +NFTMF+ LFGEK++GSDPEE++ NAF  FD ++ GF++EE+++DL+ +MGD +T E++ + +  AP++ GK +Y  F + IK GK +E
Sbjct:   12 RAQRATSNVFGMFPQNQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPSDKELQEMLSESPGPLNFTMFINLFGEKLSGSDPEESLRNAFKMFDSDNKGFLAEEYIKDLLENMGDNFTAEEIRQTWKEAPLTGGKLDYEAFVRQIKRGKDDE 167          
BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000026825.1 (SMESG000026825.1)

HSP 1 Score: 174.096 bits (440), Expect = 2.900e-53
Identity = 79/156 (50.64%), Postives = 113/156 (72.44%), Query Frame = 3
Query:  141 RTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEE 608
            R QR TSNVF MF + QIQEFKEAF M+DQN+DGFID +DL +M  SLG+   +  + EML+++ G +NFTMF+ LFGEK+ G+DPEE + NAF  FD  +  ++ EE+++DL+ +MGD +T E++ + +  AP+S GK +Y  F + IK GK++E
Sbjct:   12 RAQRATSNVFGMFPQQQIQEFKEAFTMVDQNRDGFIDIEDLKDMYASLGRTPTDKELREMLEESPGPLNFTMFINLFGEKLNGTDPEEALRNAFKMFDSNNKEYLEEEYIKDLLQNMGDNFTPEEIRQTWKEAPLSGGKLDYEAFVRQIKRGKEDE 167          
BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000034095.1 (SMESG000034095.1)

HSP 1 Score: 109.383 bits (272), Expect = 9.382e-29
Identity = 59/142 (41.55%), Postives = 89/142 (62.68%), Query Frame = 3
Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 146          
BLAST of Myosin regulatory light chain vs. Planmine SMEST
Match: SMESG000010769.1 (SMESG000010769.1)

HSP 1 Score: 109.383 bits (272), Expect = 9.382e-29
Identity = 59/142 (41.55%), Postives = 89/142 (62.68%), Query Frame = 3
Query:  177 FSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLGKDANEAYIEEMLKQ----AVGTINFTMFLTLFGEKMTGSDPEETILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMI 587
             +E QI EFKEAF++ D++ DG I   +L  ++ SLG++  EA +++M+ +      GTI+F  FLT+   KM  +D EE I  AF  FD +  GF+S   LR +MT++G++ TDE+VDE+   A I  +G+ NY EF  M+
Sbjct:    5 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMM 146          
The following BLAST results are available for this feature:
BLAST of Myosin regulatory light chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MYL12B2.534e-6963.92myosin light chain 12B [Source:HGNC Symbol;Acc:HGN... [more]
MYL12B2.534e-6963.92myosin light chain 12B [Source:HGNC Symbol;Acc:HGN... [more]
MYL12B2.534e-6963.92myosin light chain 12B [Source:HGNC Symbol;Acc:HGN... [more]
MYL12B2.534e-6963.92myosin light chain 12B [Source:HGNC Symbol;Acc:HGN... [more]
MYL12B2.534e-6963.92myosin light chain 12B [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mlc-41.257e-7262.57Myosin regulatory light chain [Source:UniProtKB/S... [more]
mlc-11.117e-3744.59Myosin regulatory light chain 1 [Source:UniProtKB... [more]
mlc-21.337e-3744.59Myosin regulatory light chain 2 [Source:UniProtKB... [more]
cmd-18.948e-2941.55Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
cmd-12.640e-2040.91Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sqh8.858e-6857.56gene:FBgn0003514 transcript:FBtr0339504[more]
sqh8.858e-6857.56gene:FBgn0003514 transcript:FBtr0339503[more]
sqh8.858e-6857.56gene:FBgn0003514 transcript:FBtr0070877[more]
sqh8.858e-6857.56gene:FBgn0003514 transcript:FBtr0339502[more]
sqh8.858e-6857.56gene:FBgn0003514 transcript:FBtr0345294[more]
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BLAST of Myosin regulatory light chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myl12.21.054e-7163.91myosin, light chain 12, genome duplicate 2 [Source... [more]
myl12.21.054e-7163.91myosin, light chain 12, genome duplicate 2 [Source... [more]
myl9a2.182e-7163.31myosin, light chain 9a, regulatory [Source:ZFIN;Ac... [more]
myl9a2.182e-7163.31myosin, light chain 9a, regulatory [Source:ZFIN;Ac... [more]
myl9a2.182e-7163.31myosin, light chain 9a, regulatory [Source:ZFIN;Ac... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myl95.203e-7063.92myosin light chain 9 [Source:NCBI gene;Acc:1001248... [more]
myl12b6.603e-6863.29myosin light chain 12B [Source:NCBI gene;Acc:44806... [more]
ap5z13.056e-5250.00adaptor related protein complex 5 subunit zeta 1 [... [more]
ap5z14.621e-5248.82adaptor related protein complex 5 subunit zeta 1 [... [more]
tnrc6c2.595e-4649.68trinucleotide repeat containing 6C [Source:Xenbase... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gm499091.739e-6963.92predicted gene, 49909 [Source:MGI Symbol;Acc:MGI:6... [more]
Myl12b1.739e-6963.92myosin, light chain 12B, regulatory [Source:MGI Sy... [more]
Myl12b1.739e-6963.92myosin, light chain 12B, regulatory [Source:MGI Sy... [more]
Myl96.812e-6964.10myosin, light polypeptide 9, regulatory [Source:MG... [more]
Myl12a1.833e-6762.03myosin, light chain 12A, regulatory, non-sarcomeri... [more]
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BLAST of Myosin regulatory light chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q09510|MLRH_CAEEL1.598e-7162.57Myosin regulatory light chain OS=Caenorhabditis el... [more]
sp|A4IF97|ML12B_BOVIN1.333e-6962.35Myosin regulatory light chain 12B OS=Bos taurus OX... [more]
sp|P24032|MLRN_CHICK6.704e-6963.92Myosin regulatory light chain 2, smooth muscle min... [more]
sp|P18666|ML12B_RAT1.178e-6863.92Myosin regulatory light chain 12B OS=Rattus norveg... [more]
sp|Q3THE2|ML12B_MOUSE1.217e-6863.92Myosin regulatory light chain 12B OS=Mus musculus ... [more]
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BLAST of Myosin regulatory light chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3Q2Q12.565e-8369.82Myosin regulatory light chain sqh OS=Schistocephal... [more]
A0A5K3EJQ34.846e-8371.84Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A087VX665.681e-8371.59Myosin regulatory light chain 2 smooth muscle OS=E... [more]
A0A068X1P76.985e-8371.59Myosin regulatory light chain 2 smooth muscle OS=E... [more]
W6USV81.249e-8271.84Myosin regulatory light chain sqh OS=Echinococcus ... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000048105.11.089e-7063.31pep primary_assembly:Astyanax_mexicanus-2.0:16:981... [more]
myl9b1.106e-7062.72myosin regulatory light polypeptide 9 [Source:NCBI... [more]
ENSAMXT00000046001.11.433e-7062.72myosin regulatory light chain 2, smooth muscle min... [more]
myl9a2.052e-7062.13myosin regulatory light polypeptide 9-like [Source... [more]
ENSAMXT00000051744.16.910e-4950.64pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myl9a3.615e-6458.96myosin, light chain 9a, regulatory [Source:ZFIN;Ac... [more]
CALM32.083e-2940.85calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
CALML32.336e-2940.85calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145... [more]
ENSPMAT00000006008.13.030e-2940.85pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-... [more]
CETN19.015e-2034.19centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866][more]
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BLAST of Myosin regulatory light chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
CMD13.413e-2130.77Calmodulin; Ca2+ binding protein that regulates Ca... [more]
CDC315.606e-1329.03Calcium-binding component of the spindle pole body... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO448294.359e-6559.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447678.325e-6256.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO256261.101e-4961.65Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
EDO427741.662e-4855.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359251.157e-3139.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Myosin regulatory light chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myl9a3.045e-7161.05myosin regulatory light polypeptide 9 [Source:NCBI... [more]
myl9a8.200e-7162.72myosin regulatory light polypeptide 9 [Source:NCBI... [more]
myl12.25.231e-7060.82myosin regulatory light chain 2, smooth muscle min... [more]
ENSORLT00000014284.21.780e-6963.29myosin regulatory light chain 2, smooth muscle min... [more]
MYL28.407e-4846.63myosin regulatory light chain 2, ventricular/cardi... [more]
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BLAST of Myosin regulatory light chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000000175.19.803e-12899.43SMESG000000175.1[more]
SMESG000066078.16.495e-5652.56SMESG000066078.1[more]
SMESG000026825.12.900e-5350.64SMESG000026825.1[more]
SMESG000034095.19.382e-2941.55SMESG000034095.1[more]
SMESG000010769.19.382e-2941.55SMESG000010769.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019325 ID=SMED30019325|Name=Myosin regulatory light chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1094bp
CTTGTTTAAATCCATCAATACAATTTGTATGTATTATTCATATTTTAACA
ACTAATTTCCTATTTAAGTCAAAATTTCTTTTTAATAATATCATTTAATT
AACTATGGCATCTACAAAAACAAGAAAGAAGCATAGACCAAGAACCCAAA
GATATACTAGCAATGTATTTTCAATGTTCAGTGAACCTCAGATTCAAGAA
TTTAAAGAAGCATTCAATATGATTGACCAGAATAAAGACGGCTTTATTGA
TCACGATGATCTAGTGGAAATGCTTACTTCTCTTGGCAAAGATGCCAATG
AAGCATACATTGAAGAAATGCTCAAGCAAGCGGTGGGAACAATCAATTTT
ACTATGTTCTTAACATTATTTGGTGAAAAAATGACTGGGAGTGATCCGGA
AGAAACGATTTTGAATGCGTTTGCTTGCTTTGATCCAGAGGACACGGGAT
TCGTCTCCGAAGAACATCTTCGAGATTTAATGACTTCAATGGGGGATAGA
TGGACAGACGAACAAGTCGATGAATTATTCCACGGAGCACCGATATCAAA
TGGTAAATTCAACTATAGAGAATTTACAAAAATGATTAAGCATGGAAAGA
AAGAAGAAGATTTAAATAATCCGAATCTTTAAGTGACTCAATCGTCTAAT
TGAATTTGAATAAAAGTTATTAATTTATAGTTTTTTTAAAATTAAATGTT
ATATTTCTGTTTTCATAATATTTTATTTTCACTTGGAATTTAGTTGTTTG
CAAATTTGAAATAGGATGTTAAGATGTTTATCAAATTATTATGAATCGAT
TTATTTATGTGCATATGGTATCTCGTGAATTATTAGCTTTCATTTCATGC
ATAATTGATTGATGTGGAGGTAGCTATTGAACAAATCTGTGTGTTCTTGT
TATTTGTGTGCTATTGTAGCAAAAACGGTGTCTTGTTCACATGCGAACAT
TTATTAAGACGTATGCATTGTTTACGTTTCATAGGTTAGTTTGTTTTGAT
AAAGGTGACAAATTTTATTTTCAAATTTTTTTGATAAACTTGTCCGTTTT
TGTAATTTTTTTGCAAGAGATATTGATTTAAGTCTTTAAAAAAA
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protein sequence of SMED30019325-orf-1

>SMED30019325-orf-1 ID=SMED30019325-orf-1|Name=SMED30019325-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=176bp
MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDH
DDLVEMLTSLGKDANEAYIEEMLKQAVGTINFTMFLTLFGEKMTGSDPEE
TILNAFACFDPEDTGFVSEEHLRDLMTSMGDRWTDEQVDELFHGAPISNG
KFNYREFTKMIKHGKKEEDLNNPNL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000035Category 3 cell
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0000281mitotic cytokinesis
GO:0002119nematode larval development
GO:0007110meiosis I cytokinesis
GO:0008105asymmetric protein localization
GO:0010172embryonic body morphogenesis
GO:0030010establishment of cell polarity
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0016459myosin complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 101..129
e-value: 0.15
score: 21.2
coord: 32..60
e-value: 2.5E-7
score: 40.4
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 32..60
e-value: 3.2E-8
score: 32.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 97..132
score: 12.198
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 28..63
score: 15.88
IPR002048EF-hand domainCDDcd00051EFhcoord: 32..89
e-value: 4.51887E-8
score: 45.6165
IPR002048EF-hand domainCDDcd00051EFhcoord: 105..162
e-value: 6.74931E-7
score: 42.5349
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 95..162
e-value: 2.5E-18
score: 68.1
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 14..94
e-value: 3.6E-23
score: 83.4
NoneNo IPR availablePANTHERPTHR23049:SF66MYOSIN REGULATORY LIGHT POLYPEPTIDE 9coord: 4..169
NoneNo IPR availablePANTHERPTHR23049MYOSIN REGULATORY LIGHT CHAIN 2coord: 4..169
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 41..53
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 25..164