Stromal interaction molecule 2

Overview
NameStromal interaction molecule 2
Smed IDSMED30019320
Length (bp)2475
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Stromal interaction molecule 2 (SMED30019320) t-SNE clustered cells

Violin plots show distribution of expression levels for Stromal interaction molecule 2 (SMED30019320) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Stromal interaction molecule 2 (SMED30019320) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Stromal interaction molecule 2 (SMED30019320) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019320

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019320h1SMcG0003978 dd_Smed_v4_7756_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Stromal interaction molecule 2 vs. Ensembl Human
Match: STIM2 (stromal interaction molecule 2 [Source:HGNC Symbol;Acc:HGNC:19205])

HSP 1 Score: 245.743 bits (626), Expect = 9.259e-71
Identity = 147/397 (37.03%), Postives = 239/397 (60.20%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 458          

HSP 2 Score: 118.242 bits (295), Expect = 6.296e-27
Identity = 55/151 (36.42%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  +E++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. Ensembl Human
Match: STIM2 (stromal interaction molecule 2 [Source:HGNC Symbol;Acc:HGNC:19205])

HSP 1 Score: 247.284 bits (630), Expect = 4.100e-70
Identity = 147/397 (37.03%), Postives = 239/397 (60.20%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 458          

HSP 2 Score: 117.857 bits (294), Expect = 1.421e-26
Identity = 55/151 (36.42%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  +E++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. Ensembl Human
Match: STIM2 (stromal interaction molecule 2 [Source:HGNC Symbol;Acc:HGNC:19205])

HSP 1 Score: 246.899 bits (629), Expect = 5.991e-70
Identity = 147/397 (37.03%), Postives = 239/397 (60.20%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 458          

HSP 2 Score: 118.242 bits (295), Expect = 1.204e-26
Identity = 55/151 (36.42%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  +E++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. Ensembl Human
Match: STIM2 (stromal interaction molecule 2 [Source:HGNC Symbol;Acc:HGNC:19205])

HSP 1 Score: 239.195 bits (609), Expect = 1.166e-68
Identity = 147/405 (36.30%), Postives = 239/405 (59.01%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKD--------MCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD          EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:    9 TEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEVAASYLIQAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 413          

HSP 2 Score: 117.857 bits (294), Expect = 7.286e-27
Identity = 55/151 (36.42%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  +E++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   11 EDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 161          
BLAST of Stromal interaction molecule 2 vs. Ensembl Human
Match: STIM2 (stromal interaction molecule 2 [Source:HGNC Symbol;Acc:HGNC:19205])

HSP 1 Score: 240.736 bits (613), Expect = 1.042e-67
Identity = 147/405 (36.30%), Postives = 239/405 (59.01%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKD--------MCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD          EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEVAASYLIQAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 466          

HSP 2 Score: 117.857 bits (294), Expect = 1.438e-26
Identity = 55/151 (36.42%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  +E++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. Ensembl Celegans
Match: stim-1 (Stromal interaction molecule 1 [Source:UniProtKB/Swiss-Prot;Acc:G5EF60])

HSP 1 Score: 115.931 bits (289), Expect = 1.625e-27
Identity = 100/366 (27.32%), Postives = 182/366 (49.73%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNK---NDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIA--HSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPG 1699
             Y+ I+++H  +DDD  G +D  E+  F +++++  G  +R +R NK   +D+ I+ ++LW  W  S+   WT E +++WL   V L   V+  K  KIDG  +PR A+ +  FL K+L IK+ V+R+K+ L + D++LFG  K   +  KD +L  L ++     +L         RQ +  Q K+N+L    + LT+L+    E E+    ++ E+ K             E + R  +++  +   A+          P  L+ LL+   E E  F   ++     +M+   +M ++L+KK+ S++ SL++A   +   D +D ++   K  ++++ +   E  +RW  IE L  F +    G
Sbjct:   43 GYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGS-ERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFG-YKDNNNRTKDILLAFLALLLTSLIFLYV-------RQKQKAQQKVNELS---NKLTELKCMETEFEDVQKMLNDERSKRSISDGVVNHTEMENL-RVQLEEAERRLEANSNGSQ----APLALQPLLRRTCENEMAFLEKQRQDCFKEMKEAIEMVDRLQKKQGSVLSSLKLATGAASTSDQVDSKIFALKSRMEKIHTLTRETQERWLQIESLCGFPLLYLNG 391          

HSP 2 Score: 80.1073 bits (196), Expect = 1.140e-15
Identity = 46/151 (30.46%), Postives = 80/151 (52.98%), Query Frame = 2
Query:  152 QNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH-----LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKN 589
            + I+DK+  + I DIH +MD +  G I+ +E+  F   +++++ +       K H     +T D LW++W  S    WT + +V WL  DV+L    +      I+G ++PR A  +  FL   LGI++  +R+K+   + D++LFG   N
Sbjct:   36 EKIRDKLGYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGSERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFGYKDN 186          
BLAST of Stromal interaction molecule 2 vs. Ensembl Celegans
Match: stim-1 (Stromal interaction molecule 1 [Source:UniProtKB/Swiss-Prot;Acc:G5EF60])

HSP 1 Score: 117.472 bits (293), Expect = 2.548e-27
Identity = 103/379 (27.18%), Postives = 189/379 (49.87%), Query Frame = 2
Query:  581 SKNLLSTWEETE-----AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNK---NDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIA--HSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIF 1687
            ++N+  T EE +      Y+ I+++H  +DDD  G +D  E+  F +++++  G  +R +R NK   +D+ I+ ++LW  W  S+   WT E +++WL   V L   V+  K  KIDG  +PR A+ +  FL K+L IK+ V+R+K+ L + D++LFG  K   +  KD +L  L ++     +L         RQ +  Q K+N+L    + LT+L+    E E+    ++ E+ K             E + R  +++  +   A+          P  L+ LL+   E E  F   ++     +M+   +M ++L+KK+ S++ SL++A   +   D +D ++   K  ++++ +   E  +RW  IE L  F + 
Sbjct:   26 TRNVEVTAEEEKIRDKLGYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGS-ERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFG-YKDNNNRTKDILLAFLALLLTSLIFLYV-------RQKQKAQQKVNELS---NKLTELKCMETEFEDVQKMLNDERSKRSISDGVVNHTEMENL-RVQLEEAERRLEANSNGSQ----APLALQPLLRRTCENEMAFLEKQRQDCFKEMKEAIEMVDRLQKKQGSVLSSLKLATGAASTSDQVDSKIFALKSRMEKIHTLTRETQERWLQIESLCGFPLL 387          

HSP 2 Score: 79.7221 bits (195), Expect = 2.837e-15
Identity = 45/147 (30.61%), Postives = 79/147 (53.74%), Query Frame = 2
Query:  152 QNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH-----LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            + I+DK+  + I DIH +MD +  G I+ +E+  F   +++++ +       K H     +T D LW++W  S    WT + +V WL  DV+L    +      I+G ++PR A  +  FL   LGI++  +R+K+   + D++LFG
Sbjct:   36 EKIRDKLGYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGSERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFG 182          
BLAST of Stromal interaction molecule 2 vs. Ensembl Celegans
Match: stim-1 (Stromal interaction molecule 1 [Source:UniProtKB/Swiss-Prot;Acc:G5EF60])

HSP 1 Score: 117.472 bits (293), Expect = 2.828e-27
Identity = 103/379 (27.18%), Postives = 189/379 (49.87%), Query Frame = 2
Query:  581 SKNLLSTWEETE-----AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNK---NDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIA--HSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIF 1687
            ++N+  T EE +      Y+ I+++H  +DDD  G +D  E+  F +++++  G  +R +R NK   +D+ I+ ++LW  W  S+   WT E +++WL   V L   V+  K  KIDG  +PR A+ +  FL K+L IK+ V+R+K+ L + D++LFG  K   +  KD +L  L ++     +L         RQ +  Q K+N+L    + LT+L+    E E+    ++ E+ K             E + R  +++  +   A+          P  L+ LL+   E E  F   ++     +M+   +M ++L+KK+ S++ SL++A   +   D +D ++   K  ++++ +   E  +RW  IE L  F + 
Sbjct:   26 TRNVEVTAEEEKIRDKLGYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGS-ERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFG-YKDNNNRTKDILLAFLALLLTSLIFLYV-------RQKQKAQQKVNELS---NKLTELKCMETEFEDVQKMLNDERSKRSISDGVVNHTEMENL-RVQLEEAERRLEANSNGSQ----APLALQPLLRRTCENEMAFLEKQRQDCFKEMKEAIEMVDRLQKKQGSVLSSLKLATGAASTSDQVDSKIFALKSRMEKIHTLTRETQERWLQIESLCGFPLL 387          

HSP 2 Score: 79.7221 bits (195), Expect = 2.922e-15
Identity = 45/147 (30.61%), Postives = 79/147 (53.74%), Query Frame = 2
Query:  152 QNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH-----LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            + I+DK+  + I DIH +MD +  G I+ +E+  F   +++++ +       K H     +T D LW++W  S    WT + +V WL  DV+L    +      I+G ++PR A  +  FL   LGI++  +R+K+   + D++LFG
Sbjct:   36 EKIRDKLGYEAIRDIHRDMDDDHSGSIDRNESTGFMKEDMQMRGSERTRRENKFHGDDDAITVDDLWEAWFESIERTWTNERLVEWLINDVNLPSIVEAVKAKKIDGKILPRFASPNSDFLNKELGIKSSVYRQKLRLNSLDVVLFG 182          
BLAST of Stromal interaction molecule 2 vs. Ensembl Fly
Match: Stim (gene:FBgn0045073 transcript:FBtr0303567)

HSP 1 Score: 250.366 bits (638), Expect = 3.620e-73
Identity = 151/392 (38.52%), Postives = 230/392 (58.67%), Query Frame = 2
Query:  623 DVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFD-RAKRFNKNDN-HISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYK-----------HADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTNSSF 1759
            + I +LH QLDDD +G +D  E+D+F R+ELKY   ++ R K F+ ND+ HIS +ELW  W  S V+NWT+E   DWL   V+L +YVDLFK +K+ G+ +PRLA  ++ ++   L IK+P+H++KI LKA D++LFGPP++T +  KD IL+TL++  I GCW AY Q++ +KR +  M   M  L  AE +L ++Q  L+ A  +   V++EK      L+   ++     S +S  ++ + K            A+ E  +     P QL+  LQ  YE+E      K+ +A  Q+Q+ ++ CEKLRKK+SSL+G+    H  ++DD+D+ + EA+ A+ +V ++L ER  RW  IE  +   I    GLP L +      NGG  SS 
Sbjct:  145 EAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQRAEQSLQEMQKELERARMEQENVATEK----LDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNELSRAEFELVDNCWSPPPQLQSWLQYTYELESKNHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRSIVEARNALGDVTNELQERLHRWKQIETCLGLNIVNNNGLPYLENVLYGR-NGGLQSSM 531          

HSP 2 Score: 111.309 bits (277), Expect = 3.806e-25
Identity = 48/157 (30.57%), Postives = 97/157 (61.78%), Query Frame = 2
Query:  155 NIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISE-----NIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEE 610
            +++D+  ++ I  +H ++D + +G I+  E+++F   EL+  S  +  +     N   H++   LW++W  S+V+NWTI+    WL   V L +Y  +F  + + G+ +PRLA++++ ++ N+LGI++  H++KI+ KA D++LFGP +   + W++
Sbjct:  137 SVQDRFGMEAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKD 293          
BLAST of Stromal interaction molecule 2 vs. Ensembl Fly
Match: Stim (gene:FBgn0045073 transcript:FBtr0074159)

HSP 1 Score: 250.366 bits (638), Expect = 4.076e-73
Identity = 151/392 (38.52%), Postives = 230/392 (58.67%), Query Frame = 2
Query:  623 DVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFD-RAKRFNKNDN-HISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYK-----------HADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTNSSF 1759
            + I +LH QLDDD +G +D  E+D+F R+ELKY   ++ R K F+ ND+ HIS +ELW  W  S V+NWT+E   DWL   V+L +YVDLFK +K+ G+ +PRLA  ++ ++   L IK+P+H++KI LKA D++LFGPP++T +  KD IL+TL++  I GCW AY Q++ +KR +  M   M  L  AE +L ++Q  L+ A  +   V++EK      L+   ++     S +S  ++ + K            A+ E  +     P QL+  LQ  YE+E      K+ +A  Q+Q+ ++ CEKLRKK+SSL+G+    H  ++DD+D+ + EA+ A+ +V ++L ER  RW  IE  +   I    GLP L +      NGG  SS 
Sbjct:  145 EAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQRAEQSLQEMQKELERARMEQENVATEK----LDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNELSRAEFELVDNCWSPPPQLQSWLQYTYELESKNHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRSIVEARNALGDVTNELQERLHRWKQIETCLGLNIVNNNGLPYLENVLYGR-NGGLQSSM 531          

HSP 2 Score: 111.309 bits (277), Expect = 4.354e-25
Identity = 48/157 (30.57%), Postives = 97/157 (61.78%), Query Frame = 2
Query:  155 NIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISE-----NIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEE 610
            +++D+  ++ I  +H ++D + +G I+  E+++F   EL+  S  +  +     N   H++   LW++W  S+V+NWTI+    WL   V L +Y  +F  + + G+ +PRLA++++ ++ N+LGI++  H++KI+ KA D++LFGP +   + W++
Sbjct:  137 SVQDRFGMEAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKD 293          
BLAST of Stromal interaction molecule 2 vs. Ensembl Zebrafish
Match: stim2b (stromal interaction molecule 2b [Source:ZFIN;Acc:ZDB-GENE-120404-3])

HSP 1 Score: 256.529 bits (654), Expect = 1.016e-73
Identity = 152/391 (38.87%), Postives = 247/391 (63.17%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI-------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTA 1729
            + + ++++H QLDDD DG ++  E+ EF  +++K     ++    ++ D HI+ EELWR WK S V+NWT+EDV++WLK  VEL +Y   F+++K+DG+T+PR+AA +  F+   LKI +  H++K+ LKA D +LFGPP +  Y ++KD IL+  +II +GGCW AY Q++ SK  I  M   +  L  AE++LT+LQ+RL++A+E+N  V+ EKQ +E+K++ E    K+E SR       +  ++ + K+A++E   + +              +PE L++ LQ+ +E+E  ++  KK  A  Q+   KD  EK++KK+SS+ G+L +AHS +LD++D ++ EAK A+ EV + L ER  RW  IE+L  F +    GLP+L ++  A
Sbjct:   63 SLEALRHIHKQLDDDNDGAIEVNESVEFIIEDMK-QHQTNKHSNLHREDQHITVEELWRGWKISEVHNWTLEDVVEWLKESVELPQYERNFREFKVDGNTLPRIAANEPAFMSMQLKILDQRHKQKLNLKALDAVLFGPPLRHHYHWLKDFILMISIIIGVGGCWFAYIQNKSSKIHISQMMKDLESLQHAEESLTELQSRLEKAQEENRTVAVEKQNLEQKMRDEITGAKKEASRLRELREGAECELSRLKYAEEELVQVRMALKRAEKQMQSDWSLPEALQMWLQLTHEVEVQYYNIKKQNAELQLTVAKDEAEKIKKKRSSVFGTLHVAHSSSLDEVDHKILEAKKALSEVTACLRERLHRWQQIERLCGFPVVNNSGLPSLTASLYA 452          

HSP 2 Score: 100.523 bits (249), Expect = 2.368e-21
Identity = 50/145 (34.48%), Postives = 89/145 (61.38%), Query Frame = 2
Query:  155 NIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            N  D+ +L+ +  IH ++D + DG I  +E+ EF   +++ Q   +   N+     H+T + LW+ WK S+V+NWT++DVV WL   V+L +Y + F +  ++G+ +PR+A ++ +F+   L I +  H++K+  KA D +LFGP
Sbjct:   57 NEADRFSLEALRHIHKQLDDDNDGAIEVNESVEFIIEDMK-QHQTNKHSNLHREDQHITVEELWRGWKISEVHNWTLEDVVEWLKESVELPQYERNFREFKVDGNTLPRIAANEPAFMSMQLKILDQRHKQKLNLKALDAVLFGP 200          
BLAST of Stromal interaction molecule 2 vs. Ensembl Zebrafish
Match: stim2a (tromal interaction molecule 2a [Source:ZFIN;Acc:ZDB-GENE-130530-765])

HSP 1 Score: 243.047 bits (619), Expect = 2.493e-67
Identity = 146/398 (36.68%), Postives = 248/398 (62.31%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDE-----------TFNMEVI--VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTN 1750
            + + ++++H ++DDD+DGG++  E+ EF  ++++     ++  + +  D HI+ EELWR+WKSS V+NWT EDVL WL+  VEL +Y   FK+ ++ G+T+PR+A+ +  F+   LKI++  H++K+ LKA D++LFGPP +  ++++KD +LI  +++ IGGCW A+ Q + SK  I  M   +  L  AE +L  LQ +L++A+E+   V+ EK+ +EEK++ E +  +EE +R       ++ ++ +  +A++E             +M+ +  VPE L++ LQ+ +E+E  ++  KK +A  Q+   KD  EK++KK+SS++G+L +AHS +LD +D+ + EAK A+ EV + L ER  RW  IE L  F +    GLP L    TAT+    N
Sbjct:   58 SLEALQHIHREMDDDQDGGIEVEESVEFIIEDMQQQQQANKHSKLHHEDQHITLEELWRSWKSSEVHNWTQEDVLQWLREFVELPQYEKSFKELRVSGNTLPRIASNEPSFMSSYLKIQDQQHKQKLKLKALDVVLFGPPIRPPHNWLKDVLLIVSIVMGIGGCWFAHVQSKSSKVHISKMMKDLESLQRAEQSLRDLQEQLEKAQEEKRTVTVEKKNLEEKMRDEIQGAQEEANRLHRLRDGAVTELSRLWYAEEELEQVRGALKRAEKDMQTLWSVPESLQLWLQLTHEVEVQYYNVKKQSAEQQLITAKDEAEKIKKKRSSVLGTLHVAHSSSLDQVDQRILEAKNALAEVTACLRERLHRWQQIESLCGFPLMKNAGLPNL----TATLYPDPN 451          

HSP 2 Score: 109.768 bits (273), Expect = 4.372e-24
Identity = 53/150 (35.33%), Postives = 93/150 (62.00%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            D+ +L+ +  IH EMD ++DG I  +E+ EF   +++ Q   +    +     H+T + LW+SWK+S+V+NWT +DV+ WL   V+L +Y K F +  ++G+ +PR+A ++ SF+ + L I++  H++K+  KA D++LFGP       W
Sbjct:   55 DRFSLEALQHIHREMDDDQDGGIEVEESVEFIIEDMQQQQQANKHSKLHHEDQHITLEELWRSWKSSEVHNWTQEDVLQWLREFVELPQYEKSFKELRVSGNTLPRIASNEPSFMSSYLKIQDQQHKQKLKLKALDVVLFGPPIRPPHNW 204          
BLAST of Stromal interaction molecule 2 vs. Ensembl Zebrafish
Match: stim1b (stromal interaction molecule 1b [Source:ZFIN;Acc:ZDB-GENE-060531-4])

HSP 1 Score: 228.409 bits (581), Expect = 2.840e-64
Identity = 140/390 (35.90%), Postives = 227/390 (58.21%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I+++H  +DDD DG VD  E D F R++LKY     +   F++ D  I+ E++W +WK+S VYNWT+E+  +WL   VEL +YVD F+K  I G  +PRLA  +   L+  LKI +  H +K+ LKA D +LFGPP   +S +KD +L+  +II +GGCW AY Q+R  +  +  M   ++ L  AE +L  +Q +L  A+E++  V  EK  +E +++SE E  KEE  R       + +++ + K+A+ E   + + +               P+ L+  LQ+ +E+E  ++  KK +A  Q+   K+  EK++KK++S+ G+  +AHS +LDD+D ++  AK A+ EV + L E+  RW  IE L  F I   PG+ +L +A 
Sbjct:   58 SFEAIRSIHKDMDDDADGSVDVTETDGFLREDLKYHDPKGKHNSFHRADLLITVEDMWNSWKASEVYNWTVEEAEEWLTSFVELPQYVDSFRKNVISGKDLPRLAVKNATLLVSVLKIPDRSHAQKLQLKALDTVLFGPPVSRHSHLKDLMLVVSIIIGVGGCWFAYIQNRNYRDHMGKMIKDLDGLQRAEQSLLDMQQKLQIAQEEHHTVEEEKVNLEREMRSEIEAAKEEAQRLRELREGTENELSRRKYAEQELDQVRMALKKAERELELRSGWSPPDALQKWLQLTHEVEVQYYNIKKQSAERQLIVAKEGAEKIKKKRASIFGTFHVAHSSSLDDVDHKILSAKKALGEVTAALREKLHRWQQIESLTNFNIVNNPGMSSLAAAL 447          

HSP 2 Score: 95.1301 bits (235), Expect = 9.697e-20
Identity = 49/140 (35.00%), Postives = 82/140 (58.57%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENI-KAHL--TKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            ++ + I  IH +MD + DG ++  E + F   +L+         +  +A L  T + +W SWK S+VYNWT+++   WL   V+L +Y   F KN I+G  +PRLA+ + + L+++L I + +H +K+  KA D +LFGP
Sbjct:   57 LSFEAIRSIHKDMDDDADGSVDVTETDGFLREDLKYHDPKGKHNSFHRADLLITVEDMWNSWKASEVYNWTVEEAEEWLTSFVELPQYVDSFRKNVISGKDLPRLAVKNATLLVSVLKIPDRSHAQKLQLKALDTVLFGP 196          
BLAST of Stromal interaction molecule 2 vs. Ensembl Zebrafish
Match: stim1b (stromal interaction molecule 1b [Source:ZFIN;Acc:ZDB-GENE-060531-4])

HSP 1 Score: 228.024 bits (580), Expect = 5.249e-64
Identity = 140/390 (35.90%), Postives = 227/390 (58.21%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I+++H  +DDD DG VD  E D F R++LKY     +   F++ D  I+ E++W +WK+S VYNWT+E+  +WL   VEL +YVD F+K  I G  +PRLA  +   L+  LKI +  H +K+ LKA D +LFGPP   +S +KD +L+  +II +GGCW AY Q+R  +  +  M   ++ L  AE +L  +Q +L  A+E++  V  EK  +E +++SE E  KEE  R       + +++ + K+A+ E   + + +               P+ L+  LQ+ +E+E  ++  KK +A  Q+   K+  EK++KK++S+ G+  +AHS +LDD+D ++  AK A+ EV + L E+  RW  IE L  F I   PG+ +L +A 
Sbjct:   58 SFEAIRSIHKDMDDDADGSVDVTETDGFLREDLKYHDPKGKHNSFHRADLLITVEDMWNSWKASEVYNWTVEEAEEWLTSFVELPQYVDSFRKNVISGKDLPRLAVKNATLLVSVLKIPDRSHAQKLQLKALDTVLFGPPVSRHSHLKDLMLVVSIIIGVGGCWFAYIQNRNYRDHMGKMIKDLDGLQRAEQSLLDMQQKLQIAQEEHHTVEEEKVNLEREMRSEIEAAKEEAQRLRELREGTENELSRRKYAEQELDQVRMALKKAERELELRSGWSPPDALQKWLQLTHEVEVQYYNIKKQSAERQLIVAKEGAEKIKKKRASIFGTFHVAHSSSLDDVDHKILSAKKALGEVTAALREKLHRWQQIESLTNFNIVNNPGMSSLAAAL 447          

HSP 2 Score: 95.5153 bits (236), Expect = 9.640e-20
Identity = 49/140 (35.00%), Postives = 82/140 (58.57%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENI-KAHL--TKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            ++ + I  IH +MD + DG ++  E + F   +L+         +  +A L  T + +W SWK S+VYNWT+++   WL   V+L +Y   F KN I+G  +PRLA+ + + L+++L I + +H +K+  KA D +LFGP
Sbjct:   57 LSFEAIRSIHKDMDDDADGSVDVTETDGFLREDLKYHDPKGKHNSFHRADLLITVEDMWNSWKASEVYNWTVEEAEEWLTSFVELPQYVDSFRKNVISGKDLPRLAVKNATLLVSVLKIPDRSHAQKLQLKALDTVLFGP 196          
BLAST of Stromal interaction molecule 2 vs. Ensembl Zebrafish
Match: stim1a (stromal interaction molecule 1a [Source:ZFIN;Acc:ZDB-GENE-060503-914])

HSP 1 Score: 211.46 bits (537), Expect = 2.804e-59
Identity = 145/403 (35.98%), Postives = 232/403 (57.57%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFT--ATINGGTNS 1753
            +++ I+++H Q+DDD +G VD +E D F R++L Y     +   F+ +D  IS E+LW  WKSS VYNWT+++V+ WL  +VEL +YV+  KK    G+ +PRLA  +       LKI +  H +K+ LKA D +LFG P    ++ +KD +L+  ++I +GGCW AY Q+R SK  ++ M   ++ L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E  R       + +++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK  A  Q+Q  K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F +   PGLP+L SA     +  GG  +
Sbjct:   58 SFEAIRSIHKQMDDDDNGNVDVLETDGFLREDLNYHDPKGKHNTFHGDDQFISVEDLWNAWKSSEVYNWTVDEVVKWLIDYVELTQYVEAIKKLNFSGTAMPRLAVKNNTLTQTVLKILDRSHVQKLQLKALDTVLFGAPMMNRHNHLKDFMLVVSIVIGMGGCWFAYIQNRHSKDHMKKMMKDLDSLQRAEQSLHDLQQKLQIAQEEHRSVEVEKVNLEQKLRDEINTAKQEAQRLKELREGTENELSRQKYAEEELEQVRMALKKAEKELESRSSWSPPEALQKWLQLTHEVEVQYYNIKKQNAERQLQVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQALGEVTAALRERLHRWQQIELLTGFTLVHNPGLPSLASALNLDPSFMGGRGT 460          

HSP 2 Score: 80.1073 bits (196), Expect = 3.661e-15
Identity = 35/82 (42.68%), Postives = 56/82 (68.29%), Query Frame = 2
Query:  332 LWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            LW +WK+S+VYNWT+D+VV WL   V+L++Y +   K N +G+ +PRLA+ + +    +L I + +H +K+  KA D +LFG
Sbjct:  114 LWNAWKSSEVYNWTVDEVVKWLIDYVELTQYVEAIKKLNFSGTAMPRLAVKNNTLTQTVLKILDRSHVQKLQLKALDTVLFG 195          
BLAST of Stromal interaction molecule 2 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:NCBI gene;Acc:403095])

HSP 1 Score: 228.024 bits (580), Expect = 7.102e-64
Identity = 146/404 (36.14%), Postives = 233/404 (57.67%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV-----------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L    +E  +++ + ++H Q+DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW +WK+S VYNWT+++V  WL  +VEL +Y + F+K ++ G  +PRLA  +       LK+ +   R+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL++E    K+E  R       + +++ + K+A+ E     V +                 PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PGL AL +A 
Sbjct:   32 KVLCHNADELLSFEAVMSIHKQMDDDANGNVDVEESDEFLREDLNYHDPTAKHSTFHGEDKLISVEDLWNSWKTSEVYNWTVDEVAQWLITYVELPQYEETFRKLQLSGRDMPRLAIANATMTGTLLKMTDRSQRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKDHMKKMMKDLEGLHRAEQSLHDLQERLQKAQEEHRTVEVEKVHLEKKLQNEISLAKQEAHRLRELREGTENELSRQKYAEQEELCTWVRMALKNAEKELESHSNWSAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLLAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIETLCGFQIVNNPGLHALMAAL 435          

HSP 2 Score: 94.3597 bits (233), Expect = 2.924e-19
Identity = 48/163 (29.45%), Postives = 88/163 (53.99%), Query Frame = 2
Query:  110 NLTSNVFSFINENG---QNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
             L + +F F   +     N  + ++ + +  IH +MD + +G ++ +E++EF   +L                  ++ + LW SWK S+VYNWT+D+V  WL   V+L +Y + F K  ++G  +PRLA+++ +    +L + + + R+K+  KA D +LFGP
Sbjct:   19 TLATRLFEFCRIDKVLCHNADELLSFEAVMSIHKQMDDDANGNVDVEESDEFLREDLNYHDPTAKHSTFHGEDKLISVEDLWNSWKTSEVYNWTVDEVAQWLITYVELPQYEETFRKLQLSGRDMPRLAIANATMTGTLLKMTDRSQRQKLQLKALDTVLFGP 181          
BLAST of Stromal interaction molecule 2 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:NCBI gene;Acc:403095])

HSP 1 Score: 228.409 bits (581), Expect = 4.278e-63
Identity = 146/404 (36.14%), Postives = 233/404 (57.67%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV-----------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L    +E  +++ + ++H Q+DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW +WK+S VYNWT+++V  WL  +VEL +Y + F+K ++ G  +PRLA  +       LK+ +   R+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL++E    K+E  R       + +++ + K+A+ E     V +                 PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PGL AL +A 
Sbjct:   57 KVLCHNADELLSFEAVMSIHKQMDDDANGNVDVEESDEFLREDLNYHDPTAKHSTFHGEDKLISVEDLWNSWKTSEVYNWTVDEVAQWLITYVELPQYEETFRKLQLSGRDMPRLAIANATMTGTLLKMTDRSQRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKDHMKKMMKDLEGLHRAEQSLHDLQERLQKAQEEHRTVEVEKVHLEKKLQNEISLAKQEAHRLRELREGTENELSRQKYAEQEELCTWVRMALKNAEKELESHSNWSAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLLAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIETLCGFQIVNNPGLHALMAAL 460          

HSP 2 Score: 94.3597 bits (233), Expect = 3.187e-19
Identity = 45/146 (30.82%), Postives = 82/146 (56.16%), Query Frame = 2
Query:  152 QNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
             N  + ++ + +  IH +MD + +G ++ +E++EF   +L                  ++ + LW SWK S+VYNWT+D+V  WL   V+L +Y + F K  ++G  +PRLA+++ +    +L + + + R+K+  KA D +LFGP
Sbjct:   61 HNADELLSFEAVMSIHKQMDDDANGNVDVEESDEFLREDLNYHDPTAKHSTFHGEDKLISVEDLWNSWKTSEVYNWTVDEVAQWLITYVELPQYEETFRKLQLSGRDMPRLAIANATMTGTLLKMTDRSQRQKLQLKALDTVLFGP 206          
BLAST of Stromal interaction molecule 2 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:NCBI gene;Acc:403095])

HSP 1 Score: 201.445 bits (511), Expect = 1.060e-54
Identity = 132/366 (36.07%), Postives = 209/366 (57.10%), Query Frame = 2
Query:  698 FARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV-----------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            F R++L Y     +   F+  D  IS E+LW +WK+S VYNWT+++V  WL  +VEL +Y + F+K ++ G  +PRLA  +       LK+ +   R+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL++E    K+E  R       + +++ + K+A+ E     V +                 PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PGL AL +A 
Sbjct:    5 FLREDLNYHDPTAKHSTFHGEDKLISVEDLWNSWKTSEVYNWTVDEVAQWLITYVELPQYEETFRKLQLSGRDMPRLAIANATMTGTLLKMTDRSQRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKDHMKKMMKDLEGLHRAEQSLHDLQERLQKAQEEHRTVEVEKVHLEKKLQNEISLAKQEAHRLRELREGTENELSRQKYAEQEELCTWVRMALKNAEKELESHSNWSAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLLAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIETLCGFQIVNNPGLHALMAAL 370          

HSP 2 Score: 83.1889 bits (204), Expect = 7.807e-16
Identity = 35/83 (42.17%), Postives = 55/83 (66.27%), Query Frame = 2
Query:  332 LWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            LW SWK S+VYNWT+D+V  WL   V+L +Y + F K  ++G  +PRLA+++ +    +L + + + R+K+  KA D +LFGP
Sbjct:   34 LWNSWKTSEVYNWTVDEVAQWLITYVELPQYEETFRKLQLSGRDMPRLAIANATMTGTLLKMTDRSQRQKLQLKALDTVLFGP 116          
BLAST of Stromal interaction molecule 2 vs. Ensembl Xenopus
Match: pgm1 (phosphoglucomutase 1 [Source:Xenbase;Acc:XB-GENE-948527])

HSP 1 Score: 173.711 bits (439), Expect = 6.732e-47
Identity = 114/312 (36.54%), Postives = 181/312 (58.01%), Query Frame = 2
Query:  815 YNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKI 1684
            +NWT E+ L WL   VEL +Y   F++  + G+ +PR+A  + + +I  LKI +  HR+K  LKA D++LFGPP +  ++++KD IL   ++I +GGCW AY Q+R SK  I  M   +  L  AE +L +LQ RL++A+E+N  V+ EKQ +E K+  E    K E  R       +  ++ + K+A++E   + +                VPE L+  LQ+ +E+E  ++  KK  A   +   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F I
Sbjct:   14 HNWTEEETLQWLLEFVELPQYEKNFRENSVKGTALPRIAVNEPVLMISQLKITDRSHRQKFQLKALDVVLFGPPTRPPHNWVKDFILTISIVIGVGGCWFAYSQNRTSKEHISQMMKDLEGLQKAEISLLELQDRLEKAQEENRTVAVEKQNLERKMMDEISDAKREAHRLRELREGTECELSRLKYAEEELVQVRMALKKAEKEFELRSNWSVPETLQKWLQLTHEVEVQYYNIKKQNAEMHLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLYRWQQIEKMCGFPI 325          

HSP 2 Score: 67.781 bits (164), Expect = 2.541e-11
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 2
Query:  362 YNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +NWT ++ + WL   V+L +Y K F +N++ G+ +PR+A+++   +I+ L I + +HR+K   KA D++LFGP       W
Sbjct:   14 HNWTEEETLQWLLEFVELPQYEKNFRENSVKGTALPRIAVNEPVLMISQLKITDRSHRQKFQLKALDVVLFGPPTRPPHNW 94          
BLAST of Stromal interaction molecule 2 vs. Ensembl Xenopus
Match: ENSXETT00000030975.1 (stromal interaction molecule 2-like [Source:NCBI gene;Acc:101731659])

HSP 1 Score: 173.711 bits (439), Expect = 6.732e-47
Identity = 114/312 (36.54%), Postives = 181/312 (58.01%), Query Frame = 2
Query:  815 YNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKI 1684
            +NWT E+ L WL   VEL +Y   F++  + G+ +PR+A  + + +I  LKI +  HR+K  LKA D++LFGPP +  ++++KD IL   ++I +GGCW AY Q+R SK  I  M   +  L  AE +L +LQ RL++A+E+N  V+ EKQ +E K+  E    K E  R       +  ++ + K+A++E   + +                VPE L+  LQ+ +E+E  ++  KK  A   +   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F I
Sbjct:   14 HNWTEEETLQWLLEFVELPQYEKNFRENSVKGTALPRIAVNEPVLMISQLKITDRSHRQKFQLKALDVVLFGPPTRPPHNWVKDFILTISIVIGVGGCWFAYSQNRTSKEHISQMMKDLEGLQKAEISLLELQDRLEKAQEENRTVAVEKQNLERKMMDEISDAKREAHRLRELREGTECELSRLKYAEEELVQVRMALKKAEKEFELRSNWSVPETLQKWLQLTHEVEVQYYNIKKQNAEMHLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLYRWQQIEKMCGFPI 325          

HSP 2 Score: 67.781 bits (164), Expect = 2.541e-11
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 2
Query:  362 YNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +NWT ++ + WL   V+L +Y K F +N++ G+ +PR+A+++   +I+ L I + +HR+K   KA D++LFGP       W
Sbjct:   14 HNWTEEETLQWLLEFVELPQYEKNFRENSVKGTALPRIAVNEPVLMISQLKITDRSHRQKFQLKALDVVLFGPPTRPPHNW 94          
BLAST of Stromal interaction molecule 2 vs. Ensembl Mouse
Match: Stim2 (stromal interaction molecule 2 [Source:MGI Symbol;Acc:MGI:2151156])

HSP 1 Score: 248.44 bits (633), Expect = 1.344e-70
Identity = 147/397 (37.03%), Postives = 239/397 (60.20%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTISIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRTVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 458          

HSP 2 Score: 120.939 bits (302), Expect = 1.137e-27
Identity = 56/151 (37.09%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  DE++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. Ensembl Mouse
Match: Stim2 (stromal interaction molecule 2 [Source:MGI Symbol;Acc:MGI:2151156])

HSP 1 Score: 241.506 bits (615), Expect = 3.789e-68
Identity = 147/405 (36.30%), Postives = 239/405 (59.01%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKD--------MCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD          EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTISIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRTVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEVAASYLLQAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 466          

HSP 2 Score: 120.939 bits (302), Expect = 1.257e-27
Identity = 56/151 (37.09%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  DE++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. Ensembl Mouse
Match: Stim1 (stromal interaction molecule 1 [Source:MGI Symbol;Acc:MGI:107476])

HSP 1 Score: 232.261 bits (591), Expect = 1.380e-66
Identity = 146/402 (36.32%), Postives = 236/402 (58.71%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L  + +E  +++ ++N+H  +DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW+ WKSS VYNWT+++V+ WL  +VEL +Y + F+K ++ G  +PRLA T+       LK+ +  HR+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL+ E    K+E  R       + ++  + K+A++E   +   +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PG+ +L +A 
Sbjct:   53 KPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAAL 454          

HSP 2 Score: 101.679 bits (252), Expect = 6.722e-22
Identity = 46/142 (32.39%), Postives = 86/142 (60.56%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            +K++ + + +IH  MD + +G+++ +E++EF   +L                  ++ + LWK+WK+S+VYNWT+D+V+ WL   V+L +Y + F K  + G  +PRLA+++ +    +L + + +HR+K+  KA D +LFGP
Sbjct:   61 EKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGP 202          
BLAST of Stromal interaction molecule 2 vs. Ensembl Mouse
Match: Stim1 (stromal interaction molecule 1 [Source:MGI Symbol;Acc:MGI:107476])

HSP 1 Score: 231.876 bits (590), Expect = 3.706e-65
Identity = 146/402 (36.32%), Postives = 236/402 (58.71%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L  + +E  +++ ++N+H  +DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW+ WKSS VYNWT+++V+ WL  +VEL +Y + F+K ++ G  +PRLA T+       LK+ +  HR+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL+ E    K+E  R       + ++  + K+A++E   +   +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PG+ +L +A 
Sbjct:   53 KPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAAL 454          

HSP 2 Score: 101.293 bits (251), Expect = 1.377e-21
Identity = 46/142 (32.39%), Postives = 86/142 (60.56%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            +K++ + + +IH  MD + +G+++ +E++EF   +L                  ++ + LWK+WK+S+VYNWT+D+V+ WL   V+L +Y + F K  + G  +PRLA+++ +    +L + + +HR+K+  KA D +LFGP
Sbjct:   61 EKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGP 202          
BLAST of Stromal interaction molecule 2 vs. Ensembl Mouse
Match: Stim1 (stromal interaction molecule 1 [Source:MGI Symbol;Acc:MGI:107476])

HSP 1 Score: 232.646 bits (592), Expect = 1.243e-64
Identity = 146/402 (36.32%), Postives = 236/402 (58.71%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L  + +E  +++ ++N+H  +DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW+ WKSS VYNWT+++V+ WL  +VEL +Y + F+K ++ G  +PRLA T+       LK+ +  HR+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL+ E    K+E  R       + ++  + K+A++E   +   +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PG+ +L +A 
Sbjct:   53 KPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAAL 454          

HSP 2 Score: 101.679 bits (252), Expect = 1.490e-21
Identity = 46/142 (32.39%), Postives = 86/142 (60.56%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            +K++ + + +IH  MD + +G+++ +E++EF   +L                  ++ + LWK+WK+S+VYNWT+D+V+ WL   V+L +Y + F K  + G  +PRLA+++ +    +L + + +HR+K+  KA D +LFGP
Sbjct:   61 EKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGP 202          
BLAST of Stromal interaction molecule 2 vs. UniProt/SwissProt
Match: sp|P83094|STIM_DROME (Stromal interaction molecule homolog OS=Drosophila melanogaster OX=7227 GN=Stim PE=1 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 4.086e-72
Identity = 151/392 (38.52%), Postives = 230/392 (58.67%), Query Frame = 2
Query:  623 DVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFD-RAKRFNKNDN-HISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYK-----------HADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTNSSF 1759
            + I +LH QLDDD +G +D  E+D+F R+ELKY   ++ R K F+ ND+ HIS +ELW  W  S V+NWT+E   DWL   V+L +YVDLFK +K+ G+ +PRLA  ++ ++   L IK+P+H++KI LKA D++LFGPP++T +  KD IL+TL++  I GCW AY Q++ +KR +  M   M  L  AE +L ++Q  L+ A  +   V++EK      L+   ++     S +S  ++ + K            A+ E  +     P QL+  LQ  YE+E      K+ +A  Q+Q+ ++ CEKLRKK+SSL+G+    H  ++DD+D+ + EA+ A+ +V ++L ER  RW  IE  +   I    GLP L +      NGG  SS 
Sbjct:  145 EAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQRAEQSLQEMQKELERARMEQENVATEK----LDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNELSRAEFELVDNCWSPPPQLQSWLQYTYELESKNHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRSIVEARNALGDVTNELQERLHRWKQIETCLGLNIVNNNGLPYLENVLYGR-NGGLQSSM 531          

HSP 2 Score: 111.309 bits (277), Expect = 4.365e-24
Identity = 48/157 (30.57%), Postives = 97/157 (61.78%), Query Frame = 2
Query:  155 NIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISE-----NIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEE 610
            +++D+  ++ I  +H ++D + +G I+  E+++F   EL+  S  +  +     N   H++   LW++W  S+V+NWTI+    WL   V L +Y  +F  + + G+ +PRLA++++ ++ N+LGI++  H++KI+ KA D++LFGP +   + W++
Sbjct:  137 SVQDRFGMEAIASLHRQLDDDDNGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKLHKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKD 293          
BLAST of Stromal interaction molecule 2 vs. UniProt/SwissProt
Match: sp|P83093|STIM2_MOUSE (Stromal interaction molecule 2 OS=Mus musculus OX=10090 GN=Stim2 PE=1 SV=2)

HSP 1 Score: 248.44 bits (633), Expect = 9.403e-70
Identity = 147/397 (37.03%), Postives = 239/397 (60.20%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTISIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRTVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 458          

HSP 2 Score: 120.939 bits (302), Expect = 7.955e-27
Identity = 56/151 (37.09%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  DE++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. UniProt/SwissProt
Match: sp|Q9P246|STIM2_HUMAN (Stromal interaction molecule 2 OS=Homo sapiens OX=9606 GN=STIM2 PE=1 SV=2)

HSP 1 Score: 247.284 bits (630), Expect = 1.969e-69
Identity = 147/397 (37.03%), Postives = 239/397 (60.20%), Query Frame = 2
Query:  599 TWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGP-PKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI---------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            T E+  + + ++ +H Q+DDDKDGG++  E+DEF R+++KY    ++    ++ D HI+ E+LW+ WK+S V+NWT+ED L WL   VEL +Y   F+   + G+T+PR+A  +  F+I  LKI +  HR+K+ LKA D++LFGP  +  +++MKD IL   ++I +GGCW AY Q++ SK  +  M   +  L  AE +L  LQ RL++A+E+N  V+ EKQ +E K+  E    KEE  R       +  ++ + ++A+ E   + +                VP+ L+  LQ+ +E+E  ++  K+  A  Q+   KD  EK++KK+S++ G+L +AHS +LD++D ++ EAK A+ E+ + L ER  RW  IEK+  F+I    GLP+L S+ 
Sbjct:   62 TEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSL 458          

HSP 2 Score: 117.857 bits (294), Expect = 6.826e-26
Identity = 55/151 (36.42%), Postives = 95/151 (62.91%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            +D+ +L+ +  IH +MD +KDG I  +E++EF   +++ +   +   ++     H+T + LWK WK S+V+NWT++D + WL   V+L +Y K F  NN+ G+ +PR+A+ + SF+I+ L I + +HR+K+  KA D++LFGP       W
Sbjct:   64 EDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNW 214          
BLAST of Stromal interaction molecule 2 vs. UniProt/SwissProt
Match: sp|Q13586|STIM1_HUMAN (Stromal interaction molecule 1 OS=Homo sapiens OX=9606 GN=STIM1 PE=1 SV=3)

HSP 1 Score: 232.261 bits (591), Expect = 2.146e-64
Identity = 146/402 (36.32%), Postives = 236/402 (58.71%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L  + +E  +++ ++N+H  +DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW+ WKSS VYNWT+++V+ WL  +VEL +Y + F+K ++ G  +PRLA T+       LK+ +  HR+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL+ E    K+E  R       + ++  + K+A++E   +   +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PG+ +L +A 
Sbjct:   53 KPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAAL 454          

HSP 2 Score: 102.449 bits (254), Expect = 4.479e-21
Identity = 47/142 (33.10%), Postives = 87/142 (61.27%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            +K++ + + +IH  MD + +G+++ +E++EF   +L                  ++ + LWK+WK+S+VYNWT+D+VV WL   V+L +Y + F K  ++G  +PRLA+++ +    +L + + +HR+K+  KA D +LFGP
Sbjct:   61 EKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGP 202          
BLAST of Stromal interaction molecule 2 vs. UniProt/SwissProt
Match: sp|P70302|STIM1_MOUSE (Stromal interaction molecule 1 OS=Mus musculus OX=10090 GN=Stim1 PE=1 SV=2)

HSP 1 Score: 231.876 bits (590), Expect = 2.592e-64
Identity = 146/402 (36.32%), Postives = 236/402 (58.71%), Query Frame = 2
Query:  584 KNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKT-YSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            K L  + +E  +++ ++N+H  +DDD +G VD  E+DEF R++L Y     +   F+  D  IS E+LW+ WKSS VYNWT+++V+ WL  +VEL +Y + F+K ++ G  +PRLA T+       LK+ +  HR+K+ LKA D +LFGPP  T ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ RL +A+E++  V  EK  +E+KL+ E    K+E  R       + ++  + K+A++E   +   +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F+I   PG+ +L +A 
Sbjct:   53 KPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAAL 454          

HSP 2 Score: 101.293 bits (251), Expect = 9.633e-21
Identity = 46/142 (32.39%), Postives = 86/142 (60.56%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            +K++ + + +IH  MD + +G+++ +E++EF   +L                  ++ + LWK+WK+S+VYNWT+D+V+ WL   V+L +Y + F K  + G  +PRLA+++ +    +L + + +HR+K+  KA D +LFGP
Sbjct:   61 EKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGP 202          
BLAST of Stromal interaction molecule 2 vs. TrEMBL
Match: A0A1S3HKR8 (stromal interaction molecule homolog OS=Lingula unguis OX=7574 GN=LOC106156136 PE=4 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 3.831e-87
Identity = 168/392 (42.86%), Postives = 248/392 (63.27%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXX-----XXXXXXXXXMSRDSID-DIFKYKHADDETF--------------NMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTAT 1732
             +D I  LH +LDDDK+G VD  E+DEF RDEL+YT  F+R   F+ ND  I+ +ELW  WK S VYNWT++DVLDW+ V V+L +Y + F+   +DG+ +PR+AA    FL   L IKNPVH++K+ LKA D++LFGPPKK +S++KD  L+  ++I IGGCW AY QH++S+  ++ M   ++ L  AEDALT+LQ +L  A++    V  EKQ +E K+K     ++ E  + +++R+  + D+ + K A++E                NM    P  L+  LQ+ YEIE   +  KKL+A  Q+   KD CE++RKK+S+ +GSLR+AHS ++DD+D+ + +A+ +++EV  DL ER QRW  IE+L  F I   PG+  L S    +
Sbjct:   54 GFDAISLLHGKLDDDKNGNVDLSESDEFLRDELQYTDGFERHNIFHGNDKQITVDELWVQWKQSEVYNWTVDDVLDWVTVSVDLPQYAENFRVNAVDGTVLPRIAANSNHFLSGILGIKNPVHKQKLSLKAMDVVLFGPPKKGHSYIKDIALVASLVIAIGGCWFAYIQHKYSQSHMKKMITDLDNLQRAEDALTELQEKLHRAQKSQQEVVQEKQHLEAKMKDEIQDAKLEAERLKLAREGTEADMTRLKLAEEELAQVRSALRQAERELENMNWRPPADLQDWLQLTYEIELTHYNTKKLSAERQLAAAKDACERIRKKRSAFMGSLRLAHSSSIDDVDRRIIDARSSLEEVGRDLQERLQRWRQIEELTGFAILTNPGMAQLSSKLYGS 445          

HSP 2 Score: 127.872 bits (320), Expect = 1.355e-26
Identity = 61/164 (37.20%), Postives = 101/164 (61.59%), Query Frame = 2
Query:  104 TVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNID---ISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSK 586
            T+   +  FS  +E+ +  KDK+    I+ +H ++D +K+G ++  E++EF   EL+     +   I       +T D LW  WK S+VYNWT+DDV+ W+ + VDL +Y + F  N ++G+++PR+A +   FL  ILGI+N  H++K++ KA D++LFGP K
Sbjct:   31 TLKQKAQDFSCPSEDKECNKDKLGFDAISLLHGKLDDDKNGNVDLSESDEFLRDELQYTDGFERHNIFHGNDKQITVDELWVQWKQSEVYNWTVDDVLDWVTVSVDLPQYAENFRVNAVDGTVLPRIAANSNHFLSGILGIKNPVHKQKLSLKAMDVVLFGPPK 194          
BLAST of Stromal interaction molecule 2 vs. TrEMBL
Match: R7TJX0 (SAM domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_147177 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 6.352e-86
Identity = 162/392 (41.33%), Postives = 245/392 (62.50%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIVPE--------------QLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTAT 1732
             Y+ I  LHSQ+DDDK+G +D  E+DEF R+EL+Y  +F+R  RF+ +D+HI+ ++LW  W  S VYNWT++DVLDWL   VEL +Y D F+   + G  +PRLAA    +L   L IK PVHR+KI LK+TD +LFGPPK  ++++KD  L+  ++I IGGCWLAY QH++S+  ++ +   ++ L  AEDALT+LQ++L+ AE +   V  EK+ +E +L  E    K E  R       + ++I + K A++E   + + + E              +L+  LQ+ +EIE   +  KK AA  Q+   K+MCEK+R+K+++ +GS+R+AH+ ++DD D ++   K A++EV+ DL ERS RW  IEK+  F I   PG+  L S   +T
Sbjct:    4 GYEAITRLHSQIDDDKNGNLDWEESDEFVREELEYEDNFERQARFHGSDDHITVDDLWHLWVKSDVYNWTVDDVLDWLVTQVELPQYADSFRINDVGGRILPRLAANGQ-YLTSILGIKTPVHRQKIALKSTDTVLFGPPKPGHNYVKDVALVASLLIAIGGCWLAYVQHKYSQSHLKKVMKDLDTLQRAEDALTELQSKLELAENEQRSVVEEKKSLENRLNHEVNSAKSEAERLRQTREATEEEIGRLKLAEEELVQVRMALAEAEYELERGHGGSLVELQPWLQLTHEIEMKNYEAKKEAADKQLILAKEMCEKIRRKRAAFLGSIRVAHTSSIDDTDSKIMSTKAALEEVKMDLAERSHRWQQIEKICGFNITTNPGIQYLESLLIST 394          

HSP 2 Score: 118.242 bits (295), Expect = 1.986e-24
Identity = 57/144 (39.58%), Postives = 88/144 (61.11%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSK 586
            D +  + I  +HS++D +K+G ++ +E++EF   EL  + N +          H+T D LW  W  S VYNWT+DDV+ WL   V+L +Y   F  N++ G ++PRLA ++  +L +ILGI+   HR+KIA K+ D +LFGP K
Sbjct:    1 DPLGYEAITRLHSQIDDDKNGNLDWEESDEFVREELEYEDNFERQARFHGSDDHITVDDLWHLWVKSDVYNWTVDDVLDWLVTQVELPQYADSFRINDVGGRILPRLA-ANGQYLTSILGIKTPVHRQKIALKSTDTVLFGPPK 143          
BLAST of Stromal interaction molecule 2 vs. TrEMBL
Match: A0A0L8FN83 (SAM domain-containing protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22013405mg PE=4 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 2.483e-82
Identity = 161/383 (42.04%), Postives = 245/383 (63.97%), Query Frame = 2
Query:  596 STWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQ---NTRVSSEK--QXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEV------IVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPAL 1711
            S  ++ E ++ IK LH  +DDD++G VD  E+DEF R EL+YT  F+R   F+ ND  IS E+LW++WK S VYNWT++DV+DWL  HVEL +YV  F+ +KIDGST+PR+AA+     +  + +KN VH++K+ LKA D++LFGPPKK +S++KD  LI  +II IGGCW AY QH++S+  ++ M      L IAE++L +LQ RLD+A+++   N R+   +  ++ +  +K   ++  +E+SR  I++  K   A+      E+        P  L+  LQ+ YEIE      KKLAA +Q++  +D CEKL+KK+++ +G LRIAH  ++D++DK +  A+ +++ V  DL ER  RW  IE+L  F +    GL +L
Sbjct:    6 SCLKDKENFNAIKTLHDLIDDDRNGNVDQTESDEFLRVELQYTDGFERHFTFHGNDGFISVEDLWKSWKQSEVYNWTVDDVVDWLVHHVELPQYVTNFQTHKIDGSTLPRMAASHQ--YLSTIGVKNNVHKQKLSLKAMDVVLFGPPKKGHSYIKDLTLIASLIIAIGGCWFAYLQHKYSQAHVKKMMRDWESLQIAEESLKELQERLDKAQQEQNLNDRLKDVEGIEQPDSDVKLLRKQSLDEISRGKIEEELKQVKANLRKAEKELESRQWSSAPADLQKWLQLTYEIELQHHNNKKLAAENQLREARDCCEKLKKKRNAFMGPLRIAHGNSIDEVDKRILNARASLEVVHQDLQERLGRWRAIERLCAFSVTKNSGLASL 386          

HSP 2 Score: 106.686 bits (265), Expect = 1.215e-20
Identity = 54/152 (35.53%), Postives = 90/152 (59.21%), Query Frame = 2
Query:  140 NENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSK 586
            + N   +KDK     I  +H  +D +++G ++  E++EF   EL+     +       +   ++ + LWKSWK S+VYNWT+DDVV WL   V+L +Y   F  + I+GS +PR+A S     ++ +G++N  H++K++ KA D++LFGP K
Sbjct:    2 DHNPSCLKDKENFNAIKTLHDLIDDDRNGNVDQTESDEFLRVELQYTDGFERHFTFHGNDGFISVEDLWKSWKQSEVYNWTVDDVVDWLVHHVELPQYVTNFQTHKIDGSTLPRMAASHQ--YLSTIGVKNNVHKQKLSLKAMDVVLFGPPK 151          
BLAST of Stromal interaction molecule 2 vs. TrEMBL
Match: A0A0L8FP39 (SAM domain-containing protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22013405mg PE=4 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 6.004e-81
Identity = 161/383 (42.04%), Postives = 245/383 (63.97%), Query Frame = 2
Query:  596 STWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQ---NTRVSSEK--QXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEV------IVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPAL 1711
            S  ++ E ++ IK LH  +DDD++G VD  E+DEF R EL+YT  F+R   F+ ND  IS E+LW++WK S VYNWT++DV+DWL  HVEL +YV  F+ +KIDGST+PR+AA+     +  + +KN VH++K+ LKA D++LFGPPKK +S++KD  LI  +II IGGCW AY QH++S+  ++ M      L IAE++L +LQ RLD+A+++   N R+   +  ++ +  +K   ++  +E+SR  I++  K   A+      E+        P  L+  LQ+ YEIE      KKLAA +Q++  +D CEKL+KK+++ +G LRIAH  ++D++DK +  A+ +++ V  DL ER  RW  IE+L  F +    GL +L
Sbjct:    6 SCLKDKENFNAIKTLHDLIDDDRNGNVDQTESDEFLRVELQYTDGFERHFTFHGNDGFISVEDLWKSWKQSEVYNWTVDDVVDWLVHHVELPQYVTNFQTHKIDGSTLPRMAASHQ--YLSTIGVKNNVHKQKLSLKAMDVVLFGPPKKGHSYIKDLTLIASLIIAIGGCWFAYLQHKYSQAHVKKMMRDWESLQIAEESLKELQERLDKAQQEQNLNDRLKDVEGIEQPDSDVKLLRKQSLDEISRGKIEEELKQVKANLRKAEKELESRQWSSAPADLQKWLQLTYEIELQHHNNKKLAAENQLREARDCCEKLKKKRNAFMGPLRIAHGNSIDEVDKRILNARASLEVVHQDLQERLGRWRAIERLCAFSVTKNSGLASL 386          

HSP 2 Score: 106.686 bits (265), Expect = 3.781e-20
Identity = 54/152 (35.53%), Postives = 90/152 (59.21%), Query Frame = 2
Query:  140 NENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSK 586
            + N   +KDK     I  +H  +D +++G ++  E++EF   EL+     +       +   ++ + LWKSWK S+VYNWT+DDVV WL   V+L +Y   F  + I+GS +PR+A S     ++ +G++N  H++K++ KA D++LFGP K
Sbjct:    2 DHNPSCLKDKENFNAIKTLHDLIDDDRNGNVDQTESDEFLRVELQYTDGFERHFTFHGNDGFISVEDLWKSWKQSEVYNWTVDDVVDWLVHHVELPQYVTNFQTHKIDGSTLPRMAASHQ--YLSTIGVKNNVHKQKLSLKAMDVVLFGPPK 151          
BLAST of Stromal interaction molecule 2 vs. TrEMBL
Match: W4ZB27 (SAM domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.901e-81
Identity = 157/395 (39.75%), Postives = 239/395 (60.51%), Query Frame = 2
Query:  638 LHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXX---XXXXXXXXXMSRDSIDD------------------IFKYKHADDET---FNMEV--IVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGG 1744
            LH Q+DDD DG V+P E+DEF RDELKY  DF+R  + ++ND  IS EELW+ WK S VYNWT++  + WL+  V+L +Y + F+   I+GS +PRLA  +  F+   L I +P+HR+KI LKA D++LFGPPK  ++++KD +L+  +++ +GGCW A+ QHRFS+  ++ +  +M  L  AED+L +LQ +L+ A+E +     EKQ IE++L       +     + R+ I D                  + + + A DE     + E+    P  L+  LQ+ YEIE  ++  K++AA  Q+Q  K+ CEKLR+K++SLIGS RIAH  +LD +D+ +  A+ A+ EV ++L ER+ RW+ IE + +F I   PG   L ++     NG 
Sbjct:  103 LHRQMDDDHDGDVEPSESDEFLRDELKYEDDFERHSQLHRNDKEISAEELWKGWKYSEVYNWTVDKTVKWLEDSVDLPQYAERFQNNGIEGSALPRLATNNGHFMTAILGITDPIHRQKISLKAMDVVLFGPPKPMHNYLKDWLLVISLVVALGGCWFAFIQHRFSQSHVKQLLREMESLSSAEDSLKELQEKLNVAQEDHKSAVREKQSIEKQLTDEIETAKHEARRLERERIGDDTDGNSDLATQLSLAEEELVQVRSALDEAERRLSEEINWSAPMTLQQWLQLTYEIEYRYYVSKRVAAAKQLQVAKEECEKLRRKRASLIGSFRIAHGTSLDAVDQRIVSARTALHEVTAELKERTHRWNQIESVCQFAIMNNPGFQILMASMGGIPNGA 497          

HSP 2 Score: 124.79 bits (312), Expect = 1.856e-25
Identity = 61/154 (39.61%), Postives = 92/154 (59.74%), Query Frame = 2
Query:  161 KDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIK---AHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSK---NLLSTW 604
            KD++  + I  +H +MD + DG++   E++EF   EL+ + + +    +      ++ + LWK WK S+VYNWT+D  V WL   VDL +Y + F  N I GS +PRLA ++  F+  ILGI +  HR+KI+ KA D++LFGP K   N L  W
Sbjct:   92 KDRLGKEGIMLLHRQMDDDHDGDVEPSESDEFLRDELKYEDDFERHSQLHRNDKEISAEELWKGWKYSEVYNWTVDKTVKWLEDSVDLPQYAERFQNNGIEGSALPRLATNNGHFMTAILGITDPIHRQKISLKAMDVVLFGPPKPMHNYLKDW 245          
BLAST of Stromal interaction molecule 2 vs. Ensembl Cavefish
Match: stim1a (stromal interaction molecule 1a [Source:ZFIN;Acc:ZDB-GENE-060503-914])

HSP 1 Score: 232.261 bits (591), Expect = 2.694e-67
Identity = 144/396 (36.36%), Postives = 232/396 (58.59%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATIN 1738
            +++ I+N+H Q+DDD +G VD  E D F R++L Y     +   F+ +D  IS E+LW +WKSS VYNWT+++V++WL+ +VEL +Y + F+K    G+T+PRLA  +    +  LKI +  H +K+ LKA D +LFG P   +++ +KD +L+  ++I +GGCW A+ Q+R SK  ++ M   +  L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E  R       + +++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F I   PGLP+L SA     N
Sbjct:   31 SFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFGAPMMNSHNHLKDFMLVVSIVIGMGGCWFAFIQNRHSKDHMKKMMKDLEGLQRAEQSLHDLQQKLRIAQEEHRTVEVEKVNLEQKLRDEINSAKQEAHRLRELREGTENELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPESLQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQALGEVTAALRERLNRWQQIEILTGFTIVNNPGLPSLASALNLDPN 426          

HSP 2 Score: 98.5969 bits (244), Expect = 3.589e-21
Identity = 48/139 (34.53%), Postives = 82/139 (58.99%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            ++ + I +IH +MD + +G ++  E + F   +L          +       ++ + LW SWK+SKVYNWT+D+VV WL   V+LS+Y + F K N +G+ +PRLA+ + +  + +L I + +H +K+  KA D +LFG
Sbjct:   30 LSFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFG 168          
BLAST of Stromal interaction molecule 2 vs. Ensembl Cavefish
Match: stim1a (stromal interaction molecule 1a [Source:ZFIN;Acc:ZDB-GENE-060503-914])

HSP 1 Score: 233.417 bits (594), Expect = 3.907e-66
Identity = 143/391 (36.57%), Postives = 231/391 (59.08%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I+N+H Q+DDD +G VD  E D F R++L Y     +   F+ +D  IS E+LW +WKSS VYNWT+++V++WL+ +VEL +Y + F+K    G+T+PRLA  +    +  LKI +  H +K+ LKA D +LFG P   +++ +KD +L+  ++I +GGCW A+ Q+R SK  ++ M   +  L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E  R       + +++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F I   PGLP+L SA 
Sbjct:   37 SFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFGAPMMNSHNHLKDFMLVVSIVIGMGGCWFAFIQNRHSKDHMKKMMKDLEGLQRAEQSLHDLQQKLRIAQEEHRTVEVEKVNLEQKLRDEINSAKQEAHRLRELREGTENELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPESLQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQALGEVTAALRERLNRWQQIEILTGFTIVNNPGLPSLASAL 427          

HSP 2 Score: 98.5969 bits (244), Expect = 7.082e-21
Identity = 48/139 (34.53%), Postives = 82/139 (58.99%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            ++ + I +IH +MD + +G ++  E + F   +L          +       ++ + LW SWK+SKVYNWT+D+VV WL   V+LS+Y + F K N +G+ +PRLA+ + +  + +L I + +H +K+  KA D +LFG
Sbjct:   36 LSFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFG 174          
BLAST of Stromal interaction molecule 2 vs. Ensembl Cavefish
Match: stim2b (stromal interaction molecule 2 [Source:NCBI gene;Acc:103030125])

HSP 1 Score: 234.572 bits (597), Expect = 4.978e-66
Identity = 144/389 (37.02%), Postives = 243/389 (62.47%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVI-------------VPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            + + ++++H QLDDD DGG++  E+ EF  +++K     ++    ++ D HI+ EELW+ WKSS V+NWT++D + WL+  VEL +Y   F+++++DG+T+PR+AA +  F+   LKI +  H++K+ LKA D +LFGPP +  ++++KD +L+  +II +GGCW AY Q++ SK  I  M   +  L  AE +L  LQ+RL++A+E+N  V  EKQ +E+K++ E    K+E  R       +  ++ + K+A++E   + +              VPE L++ LQ+ +E+E  ++  KK +A  Q+   K+  EK++KK+ S++G+L +AHS +LD++D ++ EAK A+ EV + L ER  RW  IEKL  F +    GLP+L ++ 
Sbjct:   55 SLEALRHIHKQLDDDNDGGIEVNESVEFIIEDMKQQ-QTNKHSNLHREDQHITMEELWKGWKSSEVHNWTLDDAIQWLRESVELPQYETNFREFRVDGNTLPRIAANEPSFMTMQLKILDQRHKQKLNLKALDAVLFGPPLRPQHNWLKDFVLMVSIIIGVGGCWFAYVQNKSSKIHISRMMKDLESLQSAEQSLMDLQSRLEKAQEENRTVEVEKQNLEQKMRDEISGAKKEAHRLRELREGAECELSRLKYAEEELVQVRMALKRAEKELQSEWSVPEALQMWLQLTHEVEVQYYNIKKQSAELQLNVAKEEAEKIKKKRGSVLGTLHVAHSSSLDEVDHKILEAKKALSEVTACLRERLHRWQQIEKLCGFPVVQNSGLPSLTASL 442          

HSP 2 Score: 103.605 bits (257), Expect = 2.342e-22
Identity = 53/155 (34.19%), Postives = 90/155 (58.06%), Query Frame = 2
Query:  155 NIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQ-----SNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            N  D+ +L+ +  IH ++D + DG I  +E+ EF   +++ Q     SN+   +    H+T + LWK WK+S+V+NWT+DD + WL   V+L +Y   F +  ++G+ +PR+A ++ SF+   L I +  H++K+  KA D +LFGP       W
Sbjct:   49 NEADRFSLEALRHIHKQLDDDNDGGIEVNESVEFIIEDMKQQQTNKHSNLHREDQ---HITMEELWKGWKSSEVHNWTLDDAIQWLRESVELPQYETNFREFRVDGNTLPRIAANEPSFMTMQLKILDQRHKQKLNLKALDAVLFGPPLRPQHNW 200          
BLAST of Stromal interaction molecule 2 vs. Ensembl Cavefish
Match: stim1a (stromal interaction molecule 1a [Source:ZFIN;Acc:ZDB-GENE-060503-914])

HSP 1 Score: 233.032 bits (593), Expect = 5.664e-66
Identity = 143/391 (36.57%), Postives = 231/391 (59.08%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I+N+H Q+DDD +G VD  E D F R++L Y     +   F+ +D  IS E+LW +WKSS VYNWT+++V++WL+ +VEL +Y + F+K    G+T+PRLA  +    +  LKI +  H +K+ LKA D +LFG P   +++ +KD +L+  ++I +GGCW A+ Q+R SK  ++ M   +  L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E  R       + +++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F I   PGLP+L SA 
Sbjct:   47 SFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFGAPMMNSHNHLKDFMLVVSIVIGMGGCWFAFIQNRHSKDHMKKMMKDLEGLQRAEQSLHDLQQKLRIAQEEHRTVEVEKVNLEQKLRDEINSAKQEAHRLRELREGTENELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPESLQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQALGEVTAALRERLNRWQQIEILTGFTIVNNPGLPSLASAL 437          

HSP 2 Score: 98.5969 bits (244), Expect = 6.612e-21
Identity = 48/139 (34.53%), Postives = 82/139 (58.99%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            ++ + I +IH +MD + +G ++  E + F   +L          +       ++ + LW SWK+SKVYNWT+D+VV WL   V+LS+Y + F K N +G+ +PRLA+ + +  + +L I + +H +K+  KA D +LFG
Sbjct:   46 LSFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFG 184          
BLAST of Stromal interaction molecule 2 vs. Ensembl Cavefish
Match: stim1a (stromal interaction molecule 1a [Source:ZFIN;Acc:ZDB-GENE-060503-914])

HSP 1 Score: 233.032 bits (593), Expect = 6.887e-66
Identity = 143/391 (36.57%), Postives = 231/391 (59.08%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I+N+H Q+DDD +G VD  E D F R++L Y     +   F+ +D  IS E+LW +WKSS VYNWT+++V++WL+ +VEL +Y + F+K    G+T+PRLA  +    +  LKI +  H +K+ LKA D +LFG P   +++ +KD +L+  ++I +GGCW A+ Q+R SK  ++ M   +  L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E  R       + +++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK  A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK A+ EV + L ER  RW  IE L  F I   PGLP+L SA 
Sbjct:   55 SFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFGAPMMNSHNHLKDFMLVVSIVIGMGGCWFAFIQNRHSKDHMKKMMKDLEGLQRAEQSLHDLQQKLRIAQEEHRTVEVEKVNLEQKLRDEINSAKQEAHRLRELREGTENELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPESLQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQALGEVTAALRERLNRWQQIEILTGFTIVNNPGLPSLASAL 445          

HSP 2 Score: 98.2117 bits (243), Expect = 8.753e-21
Identity = 48/139 (34.53%), Postives = 82/139 (58.99%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            ++ + I +IH +MD + +G ++  E + F   +L          +       ++ + LW SWK+SKVYNWT+D+VV WL   V+LS+Y + F K N +G+ +PRLA+ + +  + +L I + +H +K+  KA D +LFG
Sbjct:   54 LSFEAIRNIHKQMDDDANGNVDVSETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLESYVELSQYAEAFRKMNFSGTTMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFG 192          
BLAST of Stromal interaction molecule 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002273.1 (pep scaffold:Pmarinus_7.0:GL480247:1536:7869:-1 gene:ENSPMAG00000002068.1 transcript:ENSPMAT00000002273.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 151.754 bits (382), Expect = 6.203e-41
Identity = 78/202 (38.61%), Postives = 126/202 (62.38%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARL 1219
            ++D + ++H+++DDD+DG +D  E+D F R++++Y+    + +RF+  D  I+ +E+W  W +S VYNWT++DV  WL+  VEL +Y++ F +  IDG ++PRLA T+  F+   LKI    HR K+ LKA D++LFG P     S +KD  L   V+  + GCWLA  Q R +   +  +  +++ L  AE +L  +QARL
Sbjct:    3 SFDAVSSIHARMDDDEDGTIDTRESDGFLREDMEYSDPATQHRRFHGGDQTITLKEMWAAWLASDVYNWTVQDVALWLETMVELPQYLENFLENNIDGPSLPRLAITNHTFMTTILKIPERSHRHKLQLKALDVVLFGAPLSAQRSRVKDVALAVAVVAAVTGCWLALVQRRRALEHVARVTSELDSLQKAEQSLLDMQARL 204          

HSP 2 Score: 103.219 bits (256), Expect = 2.461e-24
Identity = 50/146 (34.25%), Postives = 87/146 (59.59%), Query Frame = 2
Query:  167 KIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAH---------LTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            +++   ++ IH+ MD ++DG I++ E++ F      ++ +++ S+    H         +T   +W +W  S VYNWT+ DV  WL   V+L +Y + FL+NNI+G  +PRLA+++ +F+  IL I   +HR K+  KA D++LFG
Sbjct:    1 QLSFDAVSSIHARMDDDEDGTIDTRESDGF------LREDMEYSDPATQHRRFHGGDQTITLKEMWAAWLASDVYNWTVQDVALWLETMVELPQYLENFLENNIDGPSLPRLAITNHTFMTTILKIPERSHRHKLQLKALDVVLFG 140          
BLAST of Stromal interaction molecule 2 vs. Ensembl Nematostella
Match: EDO49682 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFY6])

HSP 1 Score: 230.335 bits (586), Expect = 4.240e-68
Identity = 145/383 (37.86%), Postives = 215/383 (56.14%), Query Frame = 2
Query:  629 IKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV--------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRS 1717
            I  +H  LDDD+DG VD  E+DEF RDEL +  D +R   F+ ND+ I+ E+LW++W SS V+NWT ++V+ WL   V L +Y + FK Y ++GS +PRLA     FL  DL +K+P HRKKI L+A D+ILFGPP   ++F+KD ++   V+I   GCW A +Q R +K+QIE     +  L  AE     LQARL  AEE + R   +K  +E +L+ E +  K E  R       + + + +   A+ E   +   +              PE+L   L + Y  E   F  K+  A  QM   KD C+++RKK++S IGS R+AH   +D++D+++  A+ A+ +V +++ ER +RW  IE L         GLP + S
Sbjct:   10 ISQIHYHLDDDRDGKVDVSESDEFMRDEL-HLSDQNRHNHFHGNDSLITVEDLWKSWVSSEVHNWTSKEVVSWLVDCVHLPQYSEAFKSYGVNGSMLPRLATKHNGFLHADLGVKDPKHRKKISLRAMDVILFGPPASGHNFLKDIVVAFSVLIATVGCWFAVHQKRRAKQQIEQTMRDLKVLQQAEKNFADLQARLAAAEEDHQRAMKQKGDLETRLQEEIDASKFEAERLQDAKDGTKEQLQRLYIAEQELMQVRKALRKAERELEYRSWKAPEELAQWLHITYTKESRHFQMKRTQALKQMTEAKDACDRIRKKRNSFIGSFRMAHDLTIDEVDQQILAARAALADVTNEMEERQRRWYQIEAL--------SGLPIMSS 383          

HSP 2 Score: 107.457 bits (267), Expect = 5.565e-25
Identity = 55/141 (39.01%), Postives = 89/141 (63.12%), Query Frame = 2
Query:  164 DKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRI--QSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGP 580
            +K+    I+ IH  +D ++DG+++  E++EF   EL +  Q+  +      + +T + LWKSW +S+V+NWT  +VV+WL   V L +Y++ F    +NGSM+PRLA     FL   LG+++  HR+KI+ +A D+ILFGP
Sbjct:    3 EKLNFAGISQIHYHLDDDRDGKVDVSESDEFMRDELHLSDQNRHNHFHGNDSLITVEDLWKSWVSSEVHNWTSKEVVSWLVDCVHLPQYSEAFKSYGVNGSMLPRLATKHNGFLHADLGVKDPKHRKKISLRAMDVILFGP 143          
BLAST of Stromal interaction molecule 2 vs. Ensembl Medaka
Match: stim2b (stromal interaction molecule 2 [Source:NCBI gene;Acc:101166502])

HSP 1 Score: 239.965 bits (611), Expect = 9.859e-68
Identity = 142/389 (36.50%), Postives = 241/389 (61.95%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNM-------------EVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            + + ++++H QLDDD DGG++  E+ EF  +++K     ++    ++ D HI+ EELW+ WK+S V+NWT +D + WL+  VEL +Y   F+ +++ G+T+PR+AA +  F+   LKI +  H++K+ LKA D +LFGPP +  +++MKD +L+  ++I +GGCW AY Q++ S+  I  M   +  L  AE +L +LQ RL++A+E+N  V+ EK+ +E+K++ E ++ K+E  R       +  ++ + K+A++E   +             E  VPE L+  LQ+ +E+E  ++  KK +A  Q+   KD  EK++KK+ S+ G+L +AHS +LD++D ++ EAK ++ EV + L ER  RW  IEKL  F I    GLP+L ++ 
Sbjct:   59 SLEALRHIHKQLDDDNDGGIEVNESVEFIIEDMKQQ-QTNKHSNLHREDQHITVEELWKGWKTSEVHNWTTDDAIQWLRESVELPQYEKNFRDFRVTGNTLPRMAANEPSFMSMQLKILDQRHKQKLNLKALDAVLFGPPLRPQHNWMKDFVLMVSIVIGVGGCWFAYMQNKSSRVHISRMMKDLESLQNAEQSLMELQGRLEKAQEENRTVAVEKENLEQKMRDEIDEAKKEAHRLRDLREGAECELSRLKYAEEELVQVRKALKQAEKEMQSERSVPEALQKWLQLTHEVEVQYYNIKKQSAEFQLCVAKDEAEKIKKKRGSVFGTLHVAHSSSLDEVDHKILEAKKSLSEVTACLRERLHRWQQIEKLCGFPIVNNSGLPSLTASL 446          

HSP 2 Score: 102.449 bits (254), Expect = 5.749e-22
Identity = 54/155 (34.84%), Postives = 87/155 (56.13%), Query Frame = 2
Query:  155 NIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQ-----SNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTW 604
            N  D+ +L+ +  IH ++D + DG I  +E+ EF   +++ Q     SN+   +    H+T + LWK WK S+V+NWT DD + WL   V+L +Y K F    + G+ +PR+A ++ SF+   L I +  H++K+  KA D +LFGP       W
Sbjct:   53 NEDDRFSLEALRHIHKQLDDDNDGGIEVNESVEFIIEDMKQQQTNKHSNLHREDQ---HITVEELWKGWKTSEVHNWTTDDAIQWLRESVELPQYEKNFRDFRVTGNTLPRMAANEPSFMSMQLKILDQRHKQKLNLKALDAVLFGPPLRPQHNW 204          
BLAST of Stromal interaction molecule 2 vs. Ensembl Medaka
Match: stim1b (stromal interaction molecule 1 [Source:NCBI gene;Acc:101164993])

HSP 1 Score: 233.802 bits (595), Expect = 1.365e-67
Identity = 144/390 (36.92%), Postives = 225/390 (57.69%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I ++H  +DDD DG VD  E DEF R++L+Y     +   F++ D HIS E++W  WKSS+VYNWT+  V +WL V VEL +Y + F+K ++DG ++PRLA  +       LKI +  H +K+ LKA D +LFGPP    +  KD +L   +++ +GGCW AY Q R S+  +  +   +  L  AE +L  LQ +L +A+E+   V  EK ++EE+L+SE +  K E  R       + +++ + K+A+ E   + + +               PE L+  LQ+ +EIE  ++  KK +A  Q+   K+  EK++KK+SSL G+  +AHS +LDD+D ++  AK A+ EV + L E+  RW  IE L  F +   PGL AL +A 
Sbjct:   43 SFEAICSIHKLMDDDADGSVDTTETDEFLREDLQYKDSKAKQNSFHRADLHISVEDMWNAWKSSIVYNWTVPQVEEWLAVSVELPQYSESFQKLQLDGRSLPRLAVKNTTLTGSLLKIVDRGHAQKLQLKALDTVLFGPPPGLRNRWKDWVLALSILMALGGCWFAYAQTRKSRDDMGKLMKDLEGLQRAEQSLLDLQEKLQQAQEEQRWVQVEKVRVEEELRSEIDSAKHEAQRLRELREGTENELSRQKYAEQELEQVRMALKKAERELESRAHWTPPEALQTWLQLTHEIEVQYYNCKKQSAERQLLQAKEGAEKIKKKRSSLFGTFHVAHSSSLDDVDHKILSAKQALAEVTAALREKLHRWQQIESLTGFALVHNPGLGALATAL 432          

HSP 2 Score: 98.5969 bits (244), Expect = 3.887e-21
Identity = 47/150 (31.33%), Postives = 87/150 (58.00%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQ---SNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEE 610
            ++ + I  IH  MD + DG +++ E +EF   +L+ +   +  +       H++ + +W +WK+S VYNWT+  V  WL + V+L +Y++ F K  ++G  +PRLA+ + +   ++L I +  H +K+  KA D +LFGP   L + W++
Sbjct:   42 LSFEAICSIHKLMDDDADGSVDTTETDEFLREDLQYKDSKAKQNSFHRADLHISVEDMWNAWKSSIVYNWTVPQVEEWLAVSVELPQYSESFQKLQLDGRSLPRLAVKNTTLTGSLLKIVDRGHAQKLQLKALDTVLFGPPPGLRNRWKD 191          
BLAST of Stromal interaction molecule 2 vs. Ensembl Medaka
Match: stim1a (stromal interaction molecule 1 [Source:NCBI gene;Acc:101156837])

HSP 1 Score: 234.572 bits (597), Expect = 2.109e-66
Identity = 146/400 (36.50%), Postives = 235/400 (58.75%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFT--ATINGG 1744
            +++ I+++H Q+DDD +G VD +E D F R++L Y     +   F+ +D  IS E+LW  WK S VYNWT+++++ WL   VEL +YVD F+K   +GST+PRLA  +    +  LKI +  H +K+ LKA D +LFG P    ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E SR       +++++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK +A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK ++ EV + L ER QRW  IE L  F I   PGL +L +A     T  GG
Sbjct:   54 SFEAIRSIHKQMDDDANGNVDIVETDGFLREDLNYHDPKAKHNTFHGDDQFISVEDLWNAWKGSEVYNWTVDEMVKWLITCVELPQYVDAFRKMNFNGSTMPRLAVKNTTLTLSILKILDRSHVQKLQLKALDTVLFGAPLMNRHNHLKDFMLVVSIVIGMGGCWFAYIQNRYSKDHMKKMMKDLEGLQRAEQSLHDLQQKLQIAQEEHRTVEVEKVNLEQKLRDEINAAKQEASRLRELREGTVNELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPESLQKWLQLTHEVEVQYYNIKKQSAERQLLMAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQSLGEVTAALRERLQRWHQIEFLTGFAIVNNPGLSSLIAALNLDPTFMGG 453          

HSP 2 Score: 94.3597 bits (233), Expect = 1.574e-19
Identity = 47/140 (33.57%), Postives = 82/140 (58.57%), Query Frame = 2
Query:  167 KIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRI---QSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            +++ + I  IH +MD + +G ++  E + F   +L     ++  +        ++ + LW +WK S+VYNWT+D++V WL   V+L +Y   F K N NGS +PRLA+ + +  ++IL I + +H +K+  KA D +LFG
Sbjct:   52 RLSFEAIRSIHKQMDDDANGNVDIVETDGFLREDLNYHDPKAKHNTFHGDDQFISVEDLWNAWKGSEVYNWTVDEMVKWLITCVELPQYVDAFRKMNFNGSTMPRLAVKNTTLTLSILKILDRSHVQKLQLKALDTVLFG 191          
BLAST of Stromal interaction molecule 2 vs. Ensembl Medaka
Match: stim1b (stromal interaction molecule 1 [Source:NCBI gene;Acc:101164993])

HSP 1 Score: 234.572 bits (597), Expect = 2.989e-66
Identity = 144/390 (36.92%), Postives = 225/390 (57.69%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAF 1723
            +++ I ++H  +DDD DG VD  E DEF R++L+Y     +   F++ D HIS E++W  WKSS+VYNWT+  V +WL V VEL +Y + F+K ++DG ++PRLA  +       LKI +  H +K+ LKA D +LFGPP    +  KD +L   +++ +GGCW AY Q R S+  +  +   +  L  AE +L  LQ +L +A+E+   V  EK ++EE+L+SE +  K E  R       + +++ + K+A+ E   + + +               PE L+  LQ+ +EIE  ++  KK +A  Q+   K+  EK++KK+SSL G+  +AHS +LDD+D ++  AK A+ EV + L E+  RW  IE L  F +   PGL AL +A 
Sbjct:   61 SFEAICSIHKLMDDDADGSVDTTETDEFLREDLQYKDSKAKQNSFHRADLHISVEDMWNAWKSSIVYNWTVPQVEEWLAVSVELPQYSESFQKLQLDGRSLPRLAVKNTTLTGSLLKIVDRGHAQKLQLKALDTVLFGPPPGLRNRWKDWVLALSILMALGGCWFAYAQTRKSRDDMGKLMKDLEGLQRAEQSLLDLQEKLQQAQEEQRWVQVEKVRVEEELRSEIDSAKHEAQRLRELREGTENELSRQKYAEQELEQVRMALKKAERELESRAHWTPPEALQTWLQLTHEIEVQYYNCKKQSAERQLLQAKEGAEKIKKKRSSLFGTFHVAHSSSLDDVDHKILSAKQALAEVTAALREKLHRWQQIESLTGFALVHNPGLGALATAL 450          

HSP 2 Score: 98.5969 bits (244), Expect = 7.485e-21
Identity = 47/150 (31.33%), Postives = 87/150 (58.00%), Query Frame = 2
Query:  170 IALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQ---SNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEE 610
            ++ + I  IH  MD + DG +++ E +EF   +L+ +   +  +       H++ + +W +WK+S VYNWT+  V  WL + V+L +Y++ F K  ++G  +PRLA+ + +   ++L I +  H +K+  KA D +LFGP   L + W++
Sbjct:   60 LSFEAICSIHKLMDDDADGSVDTTETDEFLREDLQYKDSKAKQNSFHRADLHISVEDMWNAWKSSIVYNWTVPQVEEWLAVSVELPQYSESFQKLQLDGRSLPRLAVKNTTLTGSLLKIVDRGHAQKLQLKALDTVLFGPPPGLRNRWKD 209          
BLAST of Stromal interaction molecule 2 vs. Ensembl Medaka
Match: stim1a (stromal interaction molecule 1 [Source:NCBI gene;Acc:101156837])

HSP 1 Score: 234.572 bits (597), Expect = 4.310e-66
Identity = 146/400 (36.50%), Postives = 235/400 (58.75%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPP-KKTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSR------DSIDDIFKYKHADDETFNMEVIV---------------PEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFT--ATINGG 1744
            +++ I+++H Q+DDD +G VD +E D F R++L Y     +   F+ +D  IS E+LW  WK S VYNWT+++++ WL   VEL +YVD F+K   +GST+PRLA  +    +  LKI +  H +K+ LKA D +LFG P    ++ +KD +L+  ++I +GGCW AY Q+R+SK  ++ M   +  L  AE +L  LQ +L  A+E++  V  EK  +E+KL+ E    K+E SR       +++++ + K+A++E   + + +               PE L+  LQ+ +E+E  ++  KK +A  Q+   K+  EK++KK+++L G+  +AHS +LDD+D ++  AK ++ EV + L ER QRW  IE L  F I   PGL +L +A     T  GG
Sbjct:   54 SFEAIRSIHKQMDDDANGNVDIVETDGFLREDLNYHDPKAKHNTFHGDDQFISVEDLWNAWKGSEVYNWTVDEMVKWLITCVELPQYVDAFRKMNFNGSTMPRLAVKNTTLTLSILKILDRSHVQKLQLKALDTVLFGAPLMNRHNHLKDFMLVVSIVIGMGGCWFAYIQNRYSKDHMKKMMKDLEGLQRAEQSLHDLQQKLQIAQEEHRTVEVEKVNLEQKLRDEINAAKQEASRLRELREGTVNELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPESLQKWLQLTHEVEVQYYNIKKQSAERQLLMAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQSLGEVTAALRERLQRWHQIEFLTGFAIVNNPGLSSLIAALNLDPTFMGG 453          

HSP 2 Score: 94.3597 bits (233), Expect = 1.564e-19
Identity = 47/140 (33.57%), Postives = 82/140 (58.57%), Query Frame = 2
Query:  167 KIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRI---QSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFG 577
            +++ + I  IH +MD + +G ++  E + F   +L     ++  +        ++ + LW +WK S+VYNWT+D++V WL   V+L +Y   F K N NGS +PRLA+ + +  ++IL I + +H +K+  KA D +LFG
Sbjct:   52 RLSFEAIRSIHKQMDDDANGNVDIVETDGFLREDLNYHDPKAKHNTFHGDDQFISVEDLWNAWKGSEVYNWTVDEMVKWLITCVELPQYVDAFRKMNFNGSTMPRLAVKNTTLTLSILKILDRSHVQKLQLKALDTVLFG 191          
BLAST of Stromal interaction molecule 2 vs. Planmine SMEST
Match: SMESG000045881.1 (SMESG000045881.1)

HSP 1 Score: 559.296 bits (1440), Expect = 0.000e+0
Identity = 273/275 (99.27%), Postives = 275/275 (100.00%), Query Frame = 2
Query:  101 YTVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPR 925
            YTVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFT+GELRIQSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEY+DLFKKYKIDGSTIPR
Sbjct:   18 YTVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTNGELRIQSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYIDLFKKYKIDGSTIPR 292          

HSP 2 Score: 137.887 bits (346), Expect = 7.243e-36
Identity = 69/145 (47.59%), Postives = 97/145 (66.90%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYS 1051
            A  VI ++HS++D +KDG ++  EA+EF   EL+   + D ++       H++K+ LW++WK+S VYNWT++DV+ WL + V+L EY  +F K  I+GS IPRLA +DM FLI  L I+N  HR+KI  KA DLILFGP K   S
Sbjct:   42 ALQVINDIHSEMDINKDGEINSDEAEEFTNGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLS 183          
BLAST of Stromal interaction molecule 2 vs. Planmine SMEST
Match: SMESG000052167.1 (SMESG000052167.1)

HSP 1 Score: 515.768 bits (1327), Expect = 4.956e-177
Identity = 252/254 (99.21%), Postives = 253/254 (99.61%), Query Frame = 2
Query:  101 YTVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHV 862
            YTVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFT+GELRIQSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVH 
Sbjct:   18 YTVNLTSNVFSFINENGQNIKDKIALQVINDIHSEMDINKDGEINSDEAEEFTNGELRIQSNIDISENIKAHLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHA 271          

HSP 2 Score: 139.043 bits (349), Expect = 3.914e-35
Identity = 69/145 (47.59%), Postives = 97/145 (66.90%), Query Frame = 2
Query:  617 AYDVIKNLHSQLDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNWKSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLFLIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYS 1051
            A  VI ++HS++D +KDG ++  EA+EF   EL+   + D ++       H++K+ LW++WK+S VYNWT++DV+ WL + V+L EY  +F K  I+GS IPRLA +DM FLI  L I+N  HR+KI  KA DLILFGP K   S
Sbjct:   42 ALQVINDIHSEMDINKDGEINSDEAEEFTNGELRIQSNIDISENIKA---HLTKDLLWKSWKNSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFLINILGIRNGTHRRKIARKAKDLILFGPSKNLLS 183          
BLAST of Stromal interaction molecule 2 vs. Planmine SMEST
Match: SMESG000045892.1 (SMESG000045892.1)

HSP 1 Score: 408.683 bits (1049), Expect = 1.978e-135
Identity = 233/253 (92.09%), Postives = 235/253 (92.89%), Query Frame = 2
Query: 1040 KTYSFMKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAK--------------IAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTNSS 1756
            +TYSFMKDSILITLVI+TIGGCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKH DDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAK              IAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGG   S
Sbjct:    3 ETYSFMKDSILITLVIVTIGGCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKHTDDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKFIDCSNNFSEKYFRIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGNPKS 255          
BLAST of Stromal interaction molecule 2 vs. Planmine SMEST
Match: SMESG000045892.1 (SMESG000045892.1)

HSP 1 Score: 399.053 bits (1024), Expect = 8.015e-132
Identity = 229/248 (92.34%), Postives = 230/248 (92.74%), Query Frame = 2
Query: 1055 MKDSXXXXXXXXXXXXCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAK--------------IAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTNSS 1756
            MKDSILITLVI+TIGGCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKH DDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAK              IAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGG   S
Sbjct:    1 MKDSILITLVIVTIGGCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRVSSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKHTDDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKFIDCSNNFSEKYFRIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGNPKS 248          
BLAST of Stromal interaction molecule 2 vs. Planmine SMEST
Match: SMESG000045892.1 (SMESG000045892.1)

HSP 1 Score: 318.546 bits (815), Expect = 3.479e-101
Identity = 177/196 (90.31%), Postives = 178/196 (90.82%), Query Frame = 2
Query: 1211 ARLDEAEEQNTRVSSEKQXXXXXXXXXXXXXXXXMSRDSIDDIFKYKHADDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAK--------------IAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGTNSS 1756
            +RLDEAEEQNTRVSSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKH DDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAK              IAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGG   S
Sbjct:   29 SRLDEAEEQNTRVSSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKHTDDETFNMEVIVPEQLKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRIAHSGNLDDMDKELQEAKFIDCSNNFSEKYFRIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPGLPALRSAFTATINGGNPKS 224          
The following BLAST results are available for this feature:
BLAST of Stromal interaction molecule 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
STIM29.259e-7137.03stromal interaction molecule 2 [Source:HGNC Symbol... [more]
STIM24.100e-7037.03stromal interaction molecule 2 [Source:HGNC Symbol... [more]
STIM25.991e-7037.03stromal interaction molecule 2 [Source:HGNC Symbol... [more]
STIM21.166e-6836.30stromal interaction molecule 2 [Source:HGNC Symbol... [more]
STIM21.042e-6736.30stromal interaction molecule 2 [Source:HGNC Symbol... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
stim-11.625e-2727.32Stromal interaction molecule 1 [Source:UniProtKB/... [more]
stim-12.548e-2727.18Stromal interaction molecule 1 [Source:UniProtKB/... [more]
stim-12.828e-2727.18Stromal interaction molecule 1 [Source:UniProtKB/... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Stim3.620e-7338.52gene:FBgn0045073 transcript:FBtr0303567[more]
Stim4.076e-7338.52gene:FBgn0045073 transcript:FBtr0074159[more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
stim2b1.016e-7338.87stromal interaction molecule 2b [Source:ZFIN;Acc:Z... [more]
stim2a2.493e-6736.68tromal interaction molecule 2a [Source:ZFIN;Acc:ZD... [more]
stim1b2.840e-6435.90stromal interaction molecule 1b [Source:ZFIN;Acc:Z... [more]
stim1b5.249e-6435.90stromal interaction molecule 1b [Source:ZFIN;Acc:Z... [more]
stim1a2.804e-5935.98stromal interaction molecule 1a [Source:ZFIN;Acc:Z... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
stim17.102e-6436.14stromal interaction molecule 1 [Source:NCBI gene;A... [more]
stim14.278e-6336.14stromal interaction molecule 1 [Source:NCBI gene;A... [more]
stim11.060e-5436.07stromal interaction molecule 1 [Source:NCBI gene;A... [more]
pgm16.732e-4736.54phosphoglucomutase 1 [Source:Xenbase;Acc:XB-GENE-9... [more]
ENSXETT00000030975.16.732e-4736.54stromal interaction molecule 2-like [Source:NCBI g... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Stim21.344e-7037.03stromal interaction molecule 2 [Source:MGI Symbol;... [more]
Stim23.789e-6836.30stromal interaction molecule 2 [Source:MGI Symbol;... [more]
Stim11.380e-6636.32stromal interaction molecule 1 [Source:MGI Symbol;... [more]
Stim13.706e-6536.32stromal interaction molecule 1 [Source:MGI Symbol;... [more]
Stim11.243e-6436.32stromal interaction molecule 1 [Source:MGI Symbol;... [more]
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BLAST of Stromal interaction molecule 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P83094|STIM_DROME4.086e-7238.52Stromal interaction molecule homolog OS=Drosophila... [more]
sp|P83093|STIM2_MOUSE9.403e-7037.03Stromal interaction molecule 2 OS=Mus musculus OX=... [more]
sp|Q9P246|STIM2_HUMAN1.969e-6937.03Stromal interaction molecule 2 OS=Homo sapiens OX=... [more]
sp|Q13586|STIM1_HUMAN2.146e-6436.32Stromal interaction molecule 1 OS=Homo sapiens OX=... [more]
sp|P70302|STIM1_MOUSE2.592e-6436.32Stromal interaction molecule 1 OS=Mus musculus OX=... [more]
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BLAST of Stromal interaction molecule 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3HKR83.831e-8742.86stromal interaction molecule homolog OS=Lingula un... [more]
R7TJX06.352e-8641.33SAM domain-containing protein (Fragment) OS=Capite... [more]
A0A0L8FN832.483e-8242.04SAM domain-containing protein (Fragment) OS=Octopu... [more]
A0A0L8FP396.004e-8142.04SAM domain-containing protein (Fragment) OS=Octopu... [more]
W4ZB278.901e-8139.75SAM domain-containing protein OS=Strongylocentrotu... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
stim1a2.694e-6736.36stromal interaction molecule 1a [Source:ZFIN;Acc:Z... [more]
stim1a3.907e-6636.57stromal interaction molecule 1a [Source:ZFIN;Acc:Z... [more]
stim2b4.978e-6637.02stromal interaction molecule 2 [Source:NCBI gene;A... [more]
stim1a5.664e-6636.57stromal interaction molecule 1a [Source:ZFIN;Acc:Z... [more]
stim1a6.887e-6636.57stromal interaction molecule 1a [Source:ZFIN;Acc:Z... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000002273.16.203e-4138.61pep scaffold:Pmarinus_7.0:GL480247:1536:7869:-1 ge... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Stromal interaction molecule 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO496824.240e-6837.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Stromal interaction molecule 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
stim2b9.859e-6836.50stromal interaction molecule 2 [Source:NCBI gene;A... [more]
stim1b1.365e-6736.92stromal interaction molecule 1 [Source:NCBI gene;A... [more]
stim1a2.109e-6636.50stromal interaction molecule 1 [Source:NCBI gene;A... [more]
stim1b2.989e-6636.92stromal interaction molecule 1 [Source:NCBI gene;A... [more]
stim1a4.310e-6636.50stromal interaction molecule 1 [Source:NCBI gene;A... [more]
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BLAST of Stromal interaction molecule 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000045881.17.243e-3699.27SMESG000045881.1[more]
SMESG000052167.14.956e-17799.21SMESG000052167.1[more]
SMESG000045892.11.978e-13592.09SMESG000045892.1[more]
SMESG000045892.18.015e-13292.34SMESG000045892.1[more]
SMESG000045892.13.479e-10190.31SMESG000045892.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019320 ID=SMED30019320|Name=Stromal interaction molecule 2|organism=Schmidtea mediterranea sexual|type=transcript|length=2475bp
TATTTACTTATTAATTTAATATTTTCATGACTTGAATGTAATTATCAAGA
TGACATTTTTATTTATCAGTCGATTATTTTTAAAGTTGAGTTTATTACTT
TATACTGTAAATTTAACATCCAATGTATTTTCATTTATTAATGAAAATGG
CCAAAATATTAAAGATAAAATCGCTCTTCAAGTTATAAATGATATTCATT
CAGAAATGGATATAAATAAAGATGGCGAGATAAATTCAGATGAAGCTGAA
GAATTTACTCACGGAGAGCTGAGAATTCAATCTAATATTGATATTTCAGA
AAATATCAAAGCTCATTTAACCAAAGACTTATTGTGGAAATCGTGGAAAA
ATAGCAAAGTTTATAATTGGACAATTGACGATGTTGTCACTTGGCTAAAT
ATCGATGTTGATTTATCAGAATATACTAAAGTGTTCTTAAAAAACAACAT
TAATGGATCCATGATTCCTCGATTGGCACTATCTGATATGTCATTTTTAA
TAAACATTCTGGGAATTAGAAACGGCACTCATAGGCGGAAAATCGCTCGA
AAAGCTAAAGATTTAATTTTATTTGGGCCATCTAAAAATTTGTTAAGTAC
ATGGGAAGAAACAGAAGCGTATGATGTTATCAAAAATCTTCATTCACAAC
TTGATGATGATAAAGATGGTGGTGTGGATCCTATTGAAGCGGATGAATTT
GCACGGGATGAATTGAAATATACAGGAGATTTCGATAGAGCCAAACGTTT
CAATAAAAATGACAATCATATCAGTAAAGAAGAGCTGTGGAGAAATTGGA
AATCCAGTTTAGTTTACAATTGGACTATGGAGGACGTTCTTGATTGGTTA
AAAGTACATGTAGAACTTGGCGAATACGTTGACTTGTTCAAAAAGTACAA
AATTGATGGCTCAACTATACCAAGATTGGCAGCAACTGATATGTTATTTC
TGATTAAAGATCTAAAAATTAAAAATCCAGTTCATCGAAAAAAAATTGTT
CTTAAAGCTACAGATTTAATATTATTTGGGCCCCCGAAAAAAACATATTC
GTTCATGAAGGATTCTATTTTAATTACTCTTGTTATTATAACAATTGGAG
GCTGCTGGTTAGCCTACTATCAACACAGGTTTTCAAAAAGACAGATCGAA
ACCATGCAAATGAAAATGAATCAGTTGGGAATAGCTGAAGATGCATTAAC
AAAATTACAAGCTAGATTGGATGAAGCAGAAGAACAAAATACTCGTGTTA
GTTCAGAAAAACAGAAAATTGAAGAAAAGCTGAAATCTGAATTTGAGAAA
TTGAAAGAAGAAATGAGTCGAGATAGTATTGATGATATATTCAAGTATAA
ACATGCGGATGATGAGACATTCAATATGGAAGTAATTGTACCAGAACAAT
TGAAAATTTTGCTGCAAATGAATTATGAAATTGAAGAAAATTTTTTTACT
TTTAAAAAATTGGCTGCTCATCATCAAATGCAAAACGTTAAAGACATGTG
TGAAAAATTGAGAAAAAAGAAAAGTTCATTAATAGGATCATTGCGAATAG
CCCATTCCGGAAATCTTGATGATATGGATAAAGAACTACAGGAAGCAAAA
ATTGCTGTCGATGAAGTAAGAAGTGATTTAGTTGAAAGATCCCAAAGGTG
GGATTACATTGAAAAATTAATAAAATTCAAAATATTTCCTACTCCTGGGT
TACCGGCATTGCGATCAGCGTTCACGGCAACTATAAATGGAGGTACTAAT
TCATCGTTTCCAAAGAATTCAATGATCATTTCACCGTCTTCATCTGCACT
TAGCACGCTAGTAACTGCTAATAATAATACCGGTGCTAATGCTGTCGCTA
CCACTATATCGTCTGTCGCCGCATCCAGCTCCACAATCTCCCGTCCAAAG
AATTTACAATTGTATATGAATGGTTGTTGTTCTGGTGATTCTAGCGAGGT
AACCGATACACCTTCTAGTGTTGGTGCAATCGATGCCCATTGTAGTAATA
ATATCAACAACGATTCATCTATGCTGAATGAGCTTTCAATGCAGAAGTTA
TGGAATGGATCGAATTCTTTTTCTCATTTTTGCATCGCTCCTTCTCCTTG
CTGTGCATTTTCCGATCATTGTCATAGTGCAAATGGATCAATATTAAATA
GTAGAAAAATTAGTCAAGTTTCGCTGCTCACACAGGCTGGAAATGAGTCG
CGCACCAATCTCGCCTCCCATATTAGTAAGGTTCGGCCGTTTGCTTTTCA
CTGGTTGAAAAGGCTGGATAAAACAAAAAAACCCAATAAGAAATGTAAAA
CCGCAAATAAATAGATCAATATATATATAAAATTTATATTTGAGTAGTTT
GTAGAATAATGGATTTTTATGATCCTTGTTTCCAGATCTATTATTAATTT
TATGCATTCCGTAACAGTACACCTTTGTTTCTTTTATTTGTTTTAAATAA
CACACATTGATTGGTTGAAAAAAAA
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protein sequence of SMED30019320-orf-1

>SMED30019320-orf-1 ID=SMED30019320-orf-1|Name=SMED30019320-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=755bp
MTFLFISRLFLKLSLLLYTVNLTSNVFSFINENGQNIKDKIALQVINDIH
SEMDINKDGEINSDEAEEFTHGELRIQSNIDISENIKAHLTKDLLWKSWK
NSKVYNWTIDDVVTWLNIDVDLSEYTKVFLKNNINGSMIPRLALSDMSFL
INILGIRNGTHRRKIARKAKDLILFGPSKNLLSTWEETEAYDVIKNLHSQ
LDDDKDGGVDPIEADEFARDELKYTGDFDRAKRFNKNDNHISKEELWRNW
KSSLVYNWTMEDVLDWLKVHVELGEYVDLFKKYKIDGSTIPRLAATDMLF
LIKDLKIKNPVHRKKIVLKATDLILFGPPKKTYSFMKDSILITLVIITIG
GCWLAYYQHRFSKRQIETMQMKMNQLGIAEDALTKLQARLDEAEEQNTRV
SSEKQKIEEKLKSEFEKLKEEMSRDSIDDIFKYKHADDETFNMEVIVPEQ
LKILLQMNYEIEENFFTFKKLAAHHQMQNVKDMCEKLRKKKSSLIGSLRI
AHSGNLDDMDKELQEAKIAVDEVRSDLVERSQRWDYIEKLIKFKIFPTPG
LPALRSAFTATINGGTNSSFPKNSMIISPSSSALSTLVTANNNTGANAVA
TTISSVAASSSTISRPKNLQLYMNGCCSGDSSEVTDTPSSVGAIDAHCSN
NINNDSSMLNELSMQKLWNGSNSFSHFCIAPSPCCAFSDHCHSANGSILN
SRKISQVSLLTQAGNESRTNLASHISKVRPFAFHWLKRLDKTKKPNKKCK
TANK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0005246calcium channel regulator activity
GO:0005515protein binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000499cell
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0003116parenchymal cell
Vocabulary: biological process
TermDefinition
GO:0006874cellular calcium ion homeostasis
GO:0006516glycoprotein catabolic process
Vocabulary: INTERPRO
TermDefinition
IPR037608STIM
IPR001660SAM
IPR032393SOAR
IPR013761SAM/pointed_sf
IPR006588Peptide_N_glycanase_PAW_dom
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 376..424
NoneNo IPR availableCOILSCoilCoilcoord: 506..526
NoneNo IPR availableGENE3DG3DSA:1.20.5.340coord: 362..446
e-value: 2.0E-10
score: 42.9
NoneNo IPR availableGENE3DG3DSA:1.10.238.180coord: 186..259
e-value: 5.6E-23
score: 83.1
coord: 37..108
e-value: 3.7E-11
score: 45.2
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..25
score: 0.666
NoneNo IPR availableTMHMMTMhelixcoord: 340..357
IPR001660Sterile alpha motif domainSMARTSM00454SAM_4coord: 255..326
e-value: 1.5E-8
score: 44.4
coord: 104..175
e-value: 1.9E-6
score: 37.4
IPR001660Sterile alpha motif domainPFAMPF07647SAM_2coord: 257..323
e-value: 1.6E-12
score: 47.4
coord: 106..172
e-value: 1.3E-10
score: 41.2
IPR001660Sterile alpha motif domainPROSITEPS50105SAM_DOMAINcoord: 107..175
score: 13.804
IPR001660Sterile alpha motif domainPROSITEPS50105SAM_DOMAINcoord: 258..326
score: 13.13
IPR032393Stromal interaction molecule, Orai1-activating regionPFAMPF16533SOARcoord: 447..545
e-value: 4.3E-30
score: 103.7
IPR032393Stromal interaction molecule, Orai1-activating regionCDDcd11722SOARcoord: 451..540
e-value: 1.52222E-31
score: 116.19
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3DG3DSA:1.10.150.50coord: 260..327
e-value: 1.5E-22
score: 82.0
coord: 109..176
e-value: 2.0E-20
score: 75.2
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILYSSF47769SAM/Pointed domaincoord: 247..329
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILYSSF47769SAM/Pointed domaincoord: 96..177
IPR037608Stromal interaction moleculePANTHERPTHR15136FAMILY NOT NAMEDcoord: 194..582
coord: 39..178