Sperm associated antigen 6
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019318 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13
Homology
BLAST of Sperm associated antigen 6 vs. Ensembl Human
Match: SPAG6 (sperm associated antigen 6 [Source:HGNC Symbol;Acc:HGNC:11215]) HSP 1 Score: 825.083 bits (2130), Expect = 0.000e+0 Identity = 397/506 (78.46%), Postives = 451/506 (89.13%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQ +AELA RPQNIETLQ AGVM+LLR LLLDVVP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGA+AHLAQM+LNPD KLK QI SAL+Q++KHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN +TLIREIAKHTPELSQL+VNAGGVAA++D IG KGN LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQIGRHTPEH++AVA N LP LL Y+ + SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGS L EYIN IN+CYPEEIV+YYSPGYS+ LL+RV+SYQP Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVRYYSPGYSDTLLQRVDSYQP 506
BLAST of Sperm associated antigen 6 vs. Ensembl Human
Match: SPAG6 (sperm associated antigen 6 [Source:HGNC Symbol;Acc:HGNC:11215]) HSP 1 Score: 751.895 bits (1940), Expect = 0.000e+0 Identity = 363/467 (77.73%), Postives = 415/467 (88.87%), Query Frame = 2 Query: 179 AGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 AGVM+LLR LLLDVVP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGA+AHLAQM+LNPD KLK QI SAL+Q++KHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN +TLIREIAKHTPELSQL+VNAGGVAA++D IG KGN LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQIGRHTPEH++AVA N LP LL Y+ + SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGS L EYIN IN+CYPEEIV+YYSPGYS+ LL+RV+SYQP Sbjct: 15 AGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVRYYSPGYSDTLLQRVDSYQP 481 HSP 2 Score: 130.568 bits (327), Expect = 5.233e-32 Identity = 84/273 (30.77%), Postives = 152/273 (55.68%), Query Frame = 2 Query: 98 YQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDK-DDMVKKNCATLIREIAKHTPE 913 Y+K+ ++ + + + PQ + + G ++ L L D P +++ AA AL +A H+ +L+ AVV +P LV + E + K+ A+ L +AKHSPELAQ+VVD GA+ L + D +K L +++H+ LA+ VV+A P+++ C+++ D +K+ A++ + +I KH+P+L+Q VV+AG +A + + + + L +A HS +LA V+ ++ P + CL ++ +D +K A + +I +HTPE Sbjct: 74 YKKAAAFVLRAVGK--HSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPE 344
BLAST of Sperm associated antigen 6 vs. Ensembl Human
Match: SPAG6 (sperm associated antigen 6 [Source:HGNC Symbol;Acc:HGNC:11215]) HSP 1 Score: 745.347 bits (1923), Expect = 0.000e+0 Identity = 362/468 (77.35%), Postives = 412/468 (88.03%), Query Frame = 2 Query: 131 IAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSP 1534 +AELA RPQNIETLQ AGVM+LLR LLLDVVP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGA+AHLAQM+LNPD KLK QI SAL+Q++KHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN +TLIREIAKHTPELSQL+VNAGGVAA++D IG KGN LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQIGRHTPEH++AVA N LP LL Y+ + SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGS L EYIN IN+CYPEEIV + P Sbjct: 2 VAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVSKFFP 469
BLAST of Sperm associated antigen 6 vs. Ensembl Human
Match: SPAG6 (sperm associated antigen 6 [Source:HGNC Symbol;Acc:HGNC:11215]) HSP 1 Score: 713.761 bits (1841), Expect = 0.000e+0 Identity = 351/455 (77.14%), Postives = 395/455 (86.81%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGG 1426 MSQRQVLQVFEQYQK+RTQFVQ +AELA RPQNIETLQ AGVM+LLR LLLDVVP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGA+AHLAQM+LNPD KLK QI SAL+Q++KHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN +TLIREIAKHTPELSQL+VNAGGVAA++D IG KGN LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQIGRHTPEH++AVA N LP LL Y+ + SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVL P R GG Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVL--FPWIFRYTSAEGG 453
BLAST of Sperm associated antigen 6 vs. Ensembl Human
Match: SPAG6 (sperm associated antigen 6 [Source:HGNC Symbol;Acc:HGNC:11215]) HSP 1 Score: 221.861 bits (564), Expect = 3.919e-68 Identity = 107/130 (82.31%), Postives = 121/130 (93.08%), Query Frame = 2 Query: 182 GVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVP 571 GVM+LLR LLLDVVP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVP Sbjct: 117 GVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVP 246 HSP 2 Score: 128.257 bits (321), Expect = 3.897e-33 Identity = 96/256 (37.50%), Postives = 137/256 (53.52%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVV-PSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVL-RAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFP 823 MSQRQVLQVFEQYQK+RTQFVQ +AELA RPQNIETLQ AG P+ + P + AAL + L + S G + + ++S Q F +A L + + L S + G + L L + P +++ +A LG +A +N LA++VV +P LV + E + K+ AA L + KHSP LAQ +VD GA+ L + + D +K AL IA+H+ +L++ VV+A P Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGEPGARTPVAPRALSPRRLRPAALPVELLGSRS--------VGTGVRKSIHSF--QRVFGGQAERVKLPDGCGRCALSLFPSSLHTGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVP 246 HSP 3 Score: 63.929 bits (154), Expect = 9.665e-11 Identity = 35/124 (28.23%), Postives = 70/124 (56.45%), Query Frame = 2 Query: 686 GAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVI 1057 G ++ L ++L+ +++ AL ++A ++ DLAE VV+ +I P ++ L +++ KK A ++R + KH+P+L+Q IV+ G + +V + + + L Y+A H+ LS +V+ Sbjct: 117 GVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVV 240 HSP 4 Score: 62.003 bits (149), Expect = 4.534e-10 Identity = 37/113 (32.74%), Postives = 60/113 (53.10%), Query Frame = 2 Query: 608 LKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGV 946 +++ AA AL + ++ DLA+ VV + L + + K+ L + KHS LA+ +V+ ++ CL+D D VK+ A +R IA+H ELSQ +V+AG V Sbjct: 133 IQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAV 245
BLAST of Sperm associated antigen 6 vs. Ensembl Celegans
Match: ima-3 (Importin subunit alpha-3 [Source:UniProtKB/Swiss-Prot;Acc:Q19969]) HSP 1 Score: 60.077 bits (144), Expect = 2.491e-9 Identity = 71/358 (19.83%), Postives = 145/358 (40.50%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSED------LQNKSKKALKHILQKCVYLEALEP---LLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTS----GGLKKVQDMK 1450 ++ G L LV CL DP ++ +AW L IA ++ Q+VV+AGAVPL + + +L + + AL +I P ++ G + L Q + NP+ + R + + + + V I PA+ + +D + + + + E Q+++ A V +V +G + + +G + ++ + V+ S GVL+ ++ I W + I + + V ++P ++ R D + N + + +++ V L L P +L I++ V+ + +L +A + GGL K+++++ Sbjct: 104 LIGSGILPVLVQCLSSTDPNLQFEAAWALTNIASGTSEQTQAVVNAGAVPLFLQLLSCGNLNVCEQSVWALGNIIGDGPHFRDYCLELGILQPLLQFI-NPEIPIGFLRNVTWVIVNLCRCKDPAPSPAVVRTILPALSLLIHHQDTNILIDTVWALSYLTDGGNEHIQMVIEAQVVTHLVPLLGHVDVKVQTAALRAVGNIVTGTDEQTQLVLDS-GVLRFMPGLLAHYKEKINKEAVWFVSNITAGNQQQVQDVFDAGIMPMIIHLLDRGDFPTQKEAAWAISNVTISGRPNQVEQMVKLGVLRPFCAMLSCTDSQIIQVVLDGINNILKMAGEAAEQVTSEIEECGGLDKIENLQ 459 HSP 2 Score: 54.299 bits (129), Expect = 1.820e-7 Identity = 42/186 (22.58%), Postives = 83/186 (44.62%), Query Frame = 2 Query: 251 ALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDIC-KHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVV 805 AL L + ++ V++ ++ LV L D + AA + + + E Q V+D G L + L + + + + W + I N Q Q V DAG +P+++ + D ++ AA A+S++ P+ + +V G + M+ D+++ + + + I K + + AE V Sbjct: 258 ALSYLTDGGNEHIQMVIEAQVVTHLVPLLGHVDVKVQTAALRAVGNIVTGTDEQTQLVLDSGVLRFMPGLLAHYKEKINKEAVWFVSNITAGNQQQVQDVFDAGIMPMIIHLLDRGDFPTQKEAAWAISNVTISGRPNQVEQMVKLGVLRPFCAMLSCTDSQIIQVVLDGINNILKMAGEAAEQVT 443
BLAST of Sperm associated antigen 6 vs. Ensembl Celegans
Match: ima-2 (Importin subunit alpha-2 [Source:UniProtKB/Swiss-Prot;Acc:P91276]) HSP 1 Score: 55.8398 bits (133), Expect = 6.007e-8 Identity = 63/268 (23.51%), Postives = 117/268 (43.66%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAG-AVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVD---AGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 V+ CG L ALV L + V+ +AW L I + + + V+AG +PL+ L V + + A A+++I S L V+ A+ HL + V R I A + + +H A + V + ++ ++ D V+++ + + E +L +G + +V F E + N+ P + LG VA +++L+ +VI + ++ + I+ W + I T + +AV N+LP L+ Sbjct: 128 VIHCGLLQALVQALSVENERVQYEAAWALTNIVSGSTEQTIAAVEAGVTIPLIHLSVHQS-AQISEQALWAVANIAGDSSQLRDYVIKCHGVEALMHLMEKVDQLGDSHVRTIAWAFSNMCRHKNPHAPLEVLRVLSKGLVKLVQHTDRQVRQDACWAVSYLTDGPDEQIELARESGVLPHVVAFFKEAE-NLVAPALRTLGNVATGNDSLTQAVIDLGSLDEILPLMEKTRSSSIVKECCWLVSNIIAGTQKQIQAVLDANLLPVLI 393
BLAST of Sperm associated antigen 6 vs. Ensembl Fly
Match: Kap-alpha1 (gene:FBgn0024889 transcript:FBtr0074881) HSP 1 Score: 60.8474 bits (146), Expect = 2.152e-9 Identity = 57/261 (21.84%), Postives = 112/261 (42.91%), Query Frame = 2 Query: 203 PLLLDVVPS----IQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYK-KAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIRE-IAKHTPELSQLIVNAGGVAAIVDFI 967 P+ +D++ S +Q+ A ALG +A S ++ IL L++ L+ DR + A + L + + A L L L+ D V + W +GY++ Q+V+DAG LV + P + A A+ +I Q ++ A+ ++ ++ + +K++ ++ IA + + + ++ A IFP ++ ++ + +K A I + T E +V G V + DF+ Sbjct: 183 PIFIDLLSSPHDDVQEQAVWALGNIAGDSPMCRDHLLGSGILEPLLHVLSNSDRITMIRNAVWTLSNLCRGKSPPADFAKISHGLPILARLLKYTDADVLSDTCWAIGYLSDGPNDKIQAVIDAGVCRRLVELLLHPQQNVSTAALRAVGNIVTGDDQQTQVILGYNALTCISHLLHSTAETIKKESCWTISNIAAGNREQIQALINANIFPQLMVIMQTAEFKTRKEAAWAITNATSSGTHEQIHYLVQVGCVPPMCDFL 443 HSP 2 Score: 59.3066 bits (142), Expect = 6.387e-9 Identity = 50/242 (20.66%), Postives = 109/242 (45.04%), Query Frame = 2 Query: 494 SAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPD--TKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 +AWTL IA +Q + V++AGAVP+ + + P ++ A AL +I SP ++ +G + L ++ N D T ++ +++ L+ + + A+ + P + LK D V + I ++ + Q +++AG +V+ + + N+S + +G + + + ++ + +S + ++ E I + W + I E +A+ N+ P+L+ Sbjct: 158 AAWTLTNIASGTSQQTKVVIEAGAVPIFIDLLSSPHDDVQEQAVWALGNIAGDSPMCRDHLLGSGILEPLLHVLSNSDRITMIRNAVWT-LSNLCRGKSPPADFAKISHGLPILARLLKYTDADVLSDTCWAIGYLSDGPNDKIQAVIDAGVCRRLVELLLHPQQNVSTAALRAVGNIVTGDDQQTQVILGYNALTCISHLLHSTAET-IKKESCWTISNIAAGNREQIQALINANIFPQLM 397
BLAST of Sperm associated antigen 6 vs. Ensembl Fly
Match: Kap-alpha3 (gene:FBgn0027338 transcript:FBtr0301189) HSP 1 Score: 56.6102 bits (135), Expect = 4.056e-8 Identity = 73/339 (21.53%), Postives = 136/339 (40.12%), Query Frame = 2 Query: 233 QQTAALALGR--LANHSDDLALAVVKGDILPQLVYSLAEQDR-FYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNN--DCEDHILAATAWALGQIG-RHTPEHSKAVAKQNVLP---KLLRC 1219 QQ AA+ R L++ + +++ DILP LV L + + + A++ L +A + VV GA+ + L P V E + W LG I L V+ G V PLL + + R + ++C++ T + L ++ + DT + A++ + D ++V+E+ + P ++ L + + V+ + I + E +Q+++N ++ + K I + L + A +++ +VI V L I N E AWA+ + E + K+ V+P LL C Sbjct: 82 QQLAAVQAARKLLSSDKNPPINDLIQSDILPILVECLKQHNHTMLQFEAAWALTNIASGTSAQTNEVVAAGAVPLFLQLLNSPAPNVCEQAVWALGNIIGDGPLLRDFVIKHGVVQPLLSFIKPDIPITFLRNVTWVIVNLCRNKDPAPPTATIHEILPALNVLIHHTDTNILVDTVWAISYLTDGGNDQIQMVIESGVVPKLIPLLGNSEVKVQTAALRAVGNIVTGSDEQTQVVLNYDALSYFPGLLSHPKEKIRKEAVWFLSNITAGNQSQVQAVI---NVGLLPKIIENLSKGEFQTQKEAAWAISNLTISGNREQVFTLIKEGVIPPFCDLLSC 417
BLAST of Sperm associated antigen 6 vs. Ensembl Fly
Match: Kap-alpha3 (gene:FBgn0027338 transcript:FBtr0082106) HSP 1 Score: 56.6102 bits (135), Expect = 4.056e-8 Identity = 73/339 (21.53%), Postives = 136/339 (40.12%), Query Frame = 2 Query: 233 QQTAALALGR--LANHSDDLALAVVKGDILPQLVYSLAEQDR-FYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNN--DCEDHILAATAWALGQIG-RHTPEHSKAVAKQNVLP---KLLRC 1219 QQ AA+ R L++ + +++ DILP LV L + + + A++ L +A + VV GA+ + L P V E + W LG I L V+ G V PLL + + R + ++C++ T + L ++ + DT + A++ + D ++V+E+ + P ++ L + + V+ + I + E +Q+++N ++ + K I + L + A +++ +VI V L I N E AWA+ + E + K+ V+P LL C Sbjct: 82 QQLAAVQAARKLLSSDKNPPINDLIQSDILPILVECLKQHNHTMLQFEAAWALTNIASGTSAQTNEVVAAGAVPLFLQLLNSPAPNVCEQAVWALGNIIGDGPLLRDFVIKHGVVQPLLSFIKPDIPITFLRNVTWVIVNLCRNKDPAPPTATIHEILPALNVLIHHTDTNILVDTVWAISYLTDGGNDQIQMVIESGVVPKLIPLLGNSEVKVQTAALRAVGNIVTGSDEQTQVVLNYDALSYFPGLLSHPKEKIRKEAVWFLSNITAGNQSQVQAVI---NVGLLPKIIENLSKGEFQTQKEAAWAISNLTISGNREQVFTLIKEGVIPPFCDLLSC 417
BLAST of Sperm associated antigen 6 vs. Ensembl Fly
Match: Kap-alpha3 (gene:FBgn0027338 transcript:FBtr0082108) HSP 1 Score: 56.6102 bits (135), Expect = 4.056e-8 Identity = 73/339 (21.53%), Postives = 136/339 (40.12%), Query Frame = 2 Query: 233 QQTAALALGR--LANHSDDLALAVVKGDILPQLVYSLAEQDR-FYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNN--DCEDHILAATAWALGQIG-RHTPEHSKAVAKQNVLP---KLLRC 1219 QQ AA+ R L++ + +++ DILP LV L + + + A++ L +A + VV GA+ + L P V E + W LG I L V+ G V PLL + + R + ++C++ T + L ++ + DT + A++ + D ++V+E+ + P ++ L + + V+ + I + E +Q+++N ++ + K I + L + A +++ +VI V L I N E AWA+ + E + K+ V+P LL C Sbjct: 82 QQLAAVQAARKLLSSDKNPPINDLIQSDILPILVECLKQHNHTMLQFEAAWALTNIASGTSAQTNEVVAAGAVPLFLQLLNSPAPNVCEQAVWALGNIIGDGPLLRDFVIKHGVVQPLLSFIKPDIPITFLRNVTWVIVNLCRNKDPAPPTATIHEILPALNVLIHHTDTNILVDTVWAISYLTDGGNDQIQMVIESGVVPKLIPLLGNSEVKVQTAALRAVGNIVTGSDEQTQVVLNYDALSYFPGLLSHPKEKIRKEAVWFLSNITAGNQSQVQAVI---NVGLLPKIIENLSKGEFQTQKEAAWAISNLTISGNREQVFTLIKEGVIPPFCDLLSC 417
BLAST of Sperm associated antigen 6 vs. Ensembl Fly
Match: Kap-alpha3 (gene:FBgn0027338 transcript:FBtr0082107) HSP 1 Score: 56.6102 bits (135), Expect = 4.056e-8 Identity = 73/339 (21.53%), Postives = 136/339 (40.12%), Query Frame = 2 Query: 233 QQTAALALGR--LANHSDDLALAVVKGDILPQLVYSLAEQDR-FYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNN--DCEDHILAATAWALGQIG-RHTPEHSKAVAKQNVLP---KLLRC 1219 QQ AA+ R L++ + +++ DILP LV L + + + A++ L +A + VV GA+ + L P V E + W LG I L V+ G V PLL + + R + ++C++ T + L ++ + DT + A++ + D ++V+E+ + P ++ L + + V+ + I + E +Q+++N ++ + K I + L + A +++ +VI V L I N E AWA+ + E + K+ V+P LL C Sbjct: 82 QQLAAVQAARKLLSSDKNPPINDLIQSDILPILVECLKQHNHTMLQFEAAWALTNIASGTSAQTNEVVAAGAVPLFLQLLNSPAPNVCEQAVWALGNIIGDGPLLRDFVIKHGVVQPLLSFIKPDIPITFLRNVTWVIVNLCRNKDPAPPTATIHEILPALNVLIHHTDTNILVDTVWAISYLTDGGNDQIQMVIESGVVPKLIPLLGNSEVKVQTAALRAVGNIVTGSDEQTQVVLNYDALSYFPGLLSHPKEKIRKEAVWFLSNITAGNQSQVQAVI---NVGLLPKIIENLSKGEFQTQKEAAWAISNLTISGNREQVFTLIKEGVIPPFCDLLSC 417
BLAST of Sperm associated antigen 6 vs. Ensembl Zebrafish
Match: spag6 (sperm associated antigen 6 [Source:ZFIN;Acc:ZDB-GENE-040704-53]) HSP 1 Score: 824.698 bits (2129), Expect = 0.000e+0 Identity = 393/506 (77.67%), Postives = 447/506 (88.34%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQ+LQVFEQYQK+RTQFVQT+A+LA RPQN ETLQ AGVM+LLRPLLLD VP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSPELAQ+VVDCGA+DALVI LEEFDP VKE +AW +G IARHN QL+Q+VVDAG VPLLVLC+QEP++ LKR+AASALSDI KHSP+LAQTVVD GAIAHLAQM+LNPD KLKRQ+FSAL QIAKHSVD+AE+VVEAEIFPA L CLKD D+ V+KN ATLIREI KHTPELSQ+IVN GGVAA++D++G++KGN+ LPGIMMLGYVAAHSE L+M+VIVS+GV QL+ + EDH+ AA AWA GQIGRHTPEH+K VA NV PKLL YL + SEDLQ K+KK LK ILQKC YL ALEPLL +AP NILKHV+ QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGSA+ EYIN INNCYPEEIV+YYSPGYSE LLER+E YQP Sbjct: 1 MSQRQILQVFEQYQKARTQFVQTVADLATRPQNSETLQNAGVMSLLRPLLLDAVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPELAQAVVDCGAVDALVISLEEFDPGVKEAAAWAIGNIARHNGQLSQAVVDAGVVPLLVLCIQEPEIALKRVAASALSDIAKHSPELAQTVVDTGAIAHLAQMILNPDAKLKRQVFSALGQIAKHSVDVAEMVVEAEIFPAALVCLKDPDEYVRKNVATLIREITKHTPELSQMIVNVGGVAAVIDYLGDSKGNVRLPGIMMLGYVAAHSENLAMAVIVSKGVPQLAICLEEEQEDHVKAAIAWAFGQIGRHTPEHAKVVAVANVFPKLLNLYLDTESSEDLQVKAKKGLKSILQKCTYLPALEPLLYEAPSNILKHVICQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSAIQEYINAINNCYPEEIVRYYSPGYSETLLERIEKYQP 506
BLAST of Sperm associated antigen 6 vs. Ensembl Zebrafish
Match: spag6 (sperm associated antigen 6 [Source:ZFIN;Acc:ZDB-GENE-040704-53]) HSP 1 Score: 805.823 bits (2080), Expect = 0.000e+0 Identity = 386/498 (77.51%), Postives = 439/498 (88.15%), Query Frame = 2 Query: 86 VFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 VFEQYQK+RTQFVQT+A+LA RPQN ETLQ AGVM+LLRPLLLD VP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSPELAQ+VVDCGA+DALVI LEEFDP VKE +AW +G IARHN QL+Q+VVDAG VPLLVLC+QEP++ LKR+AASALSDI KHSP+LAQTVVD GAIAHLAQM+LNPD KLKRQ+FSAL QIAKHSVD+AE+VVEAEIFPA L CLKD D+ V+KN ATLIREI KHTPELSQ+IVN GGVAA++D++G++KGN+ LPGIMMLGYVAAHSE L+M+VIVS+GV QL+ + EDH+ AA AWA GQIGRHTPEH+K VA NV PKLL YL + SEDLQ K+KK LK ILQKC YL ALEPLL +AP NILKHV+ QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGSA+ EYIN INNCYPEEIV+YYSPGYSE LLER+E YQP Sbjct: 23 VFEQYQKARTQFVQTVADLATRPQNSETLQNAGVMSLLRPLLLDAVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPELAQAVVDCGAVDALVISLEEFDPGVKEAAAWAIGNIARHNGQLSQAVVDAGVVPLLVLCIQEPEIALKRVAASALSDIAKHSPELAQTVVDTGAIAHLAQMILNPDAKLKRQVFSALGQIAKHSVDVAEMVVEAEIFPAALVCLKDPDEYVRKNVATLIREITKHTPELSQMIVNVGGVAAVIDYLGDSKGNVRLPGIMMLGYVAAHSENLAMAVIVSKGVPQLAICLEEEQEDHVKAAIAWAFGQIGRHTPEHAKVVAVANVFPKLLNLYLDTESSEDLQVKAKKGLKSILQKCTYLPALEPLLYEAPSNILKHVICQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSAIQEYINAINNCYPEEIVRYYSPGYSETLLERIEKYQP 520
BLAST of Sperm associated antigen 6 vs. Ensembl Zebrafish
Match: kpna7 (karyopherin alpha 7 (importin alpha 8) [Source:ZFIN;Acc:ZDB-GENE-040426-2023]) HSP 1 Score: 61.6178 bits (148), Expect = 1.475e-9 Identity = 53/247 (21.46%), Postives = 102/247 (41.30%), Query Frame = 2 Query: 416 QSVVDCGALDALVICL-EEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAI-AHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQI 1150 + +++ G L V L DP ++ +AW+L +A + Q VV+ GAVP + + P L + A AL +I ++D G I A LA++ + R + L+ + ++ ++ P+I+ L D + + I + E +I+ AG + +V+ +G + + P + +G + + S+ L + GVL + + AWA+ I Sbjct: 110 KEIIEAGLLTRFVEFLGRNDDPTLQFEAAWSLTNVASGTSWHTQQVVEHGAVPAFIALLASPLLNISEQAVWALGNIAGDGSSYRDALIDCGVIPALLARLTPDAPVGYLRNLTWTLSNLCRNKNPFPRFSAVQQMLPSIIQLLHHSDKSILSDACWAISYLTDGPNERIDVIIKAGILTRLVELLGFEELAVVTPALRSIGNIVSGSD-LQTQAAIDAGVLSALPKLMRHQKSSVQKEAAWAVSNI 355
BLAST of Sperm associated antigen 6 vs. Ensembl Zebrafish
Match: kpna5 (karyopherin alpha 5 (importin alpha 6) [Source:NCBI gene;Acc:553205]) HSP 1 Score: 61.2326 bits (147), Expect = 1.823e-9 Identity = 60/267 (22.47%), Postives = 114/267 (42.70%), Query Frame = 2 Query: 179 AGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYK-KAASFVLRAV--AKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIRE-IAKHTPELSQLIVNAGGVAAIVDFI 967 G + + LL +Q+ A ALG +A + + V+ ILP L LA+ +R + A + L + K+ P V C L L L DP V + W L Y++ Q+V+D+G LV + D + A A+ +I Q +++ A+ L ++ +P +K++ ++ I + + V+++ +FP ++ L+ + +K A I + TPE + +V+ + + D + Sbjct: 169 TGAVPIFIELLNSEYEDVQEQAVWALGNIAGDNAECRDYVLNCGILPSLQQLLAKSNRLTTTRNAVWALSNLCRGKNPPPDFAKVSPC--LSVLSRLLFSSDPDVLADACWALSYLSDGPNDKIQTVIDSGVCRRLVELLMHSDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIKKEACWTVSNITAGNRAQIQAVIDSNVFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVSLNTIKPMCDLL 433 HSP 2 Score: 50.0618 bits (118), Expect = 5.869e-6 Identity = 60/283 (21.20%), Postives = 117/283 (41.34%), Query Frame = 2 Query: 368 ASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVP-LLVLCVQEPDLGLKRIAASALSDICKHS---PDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQ-IGRHTPEHSKAVAKQNVL 1201 A++ L +A + + + V++ GA+ + L V+E + W LG IA NA+ V++ G +P L L + L R A ALS++C+ PD A+ + ++ L++++ + D + AL+ ++ D + V+++ + ++ L D V + I +Q+I+N + ++ + K +I + + A + +VI S L I E AWA+ TPE + + N + Sbjct: 148 AAWALTNIASGTFQHTKVVIETGAVPIFIELLNSEYEDVQEQAVWALGNIAGDNAECRDYVLNCGILPSLQQLLAKSNRLTTTRNAVWALSNLCRGKNPPPDFAKV---SPCLSVLSRLLFSSDPDVLADACWALSYLSDGPNDKIQTVIDSGVCRRLVELLMHSDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIKKEACWTVSNITAGNRAQIQAVIDSNVFPVLIEILQK-AEFRTRKEAAWAITNATSGGTPEQIRYLVSLNTI 426
BLAST of Sperm associated antigen 6 vs. Ensembl Zebrafish
Match: kpna6 (karyopherin alpha 6 (importin alpha 7) [Source:ZFIN;Acc:ZDB-GENE-130530-626]) HSP 1 Score: 58.5362 bits (140), Expect = 1.171e-8 Identity = 61/267 (22.85%), Postives = 114/267 (42.70%), Query Frame = 2 Query: 179 AGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRF-YKKAASFVLRAV--AKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIRE-IAKHTPELSQLIVNAGGVAAIVDFI 967 AG + + LL +Q+ A ALG +A S V+ +ILP L+ L + R + A + L + K P + V C L L L D + + W L Y++ + Q+V+D+G LV + D + + A+ +I Q V++ A+ L ++ + ++++ ++ I + + V++A IFP ++ L+ + +K A I + TPE + +VN G + + D + Sbjct: 167 AGAVPVFIELLNSDFEDVQEQAVWALGNIAGDSAVCRDYVLNCNILPPLLVLLTKSTRLTMTRNAVWALSNLCRGKSPPPDFEKVSPC--LPVLSRLLFSSDSDLLADACWALSYLSDGPNEKIQAVIDSGVCRRLVELLMHSDYKVASPSLRAVGNIVTGDDIQTQVVLNCSALPCLLHLLSSAKESIRKEACWTISNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVNLGCIKPLCDLL 431 HSP 2 Score: 53.1434 bits (126), Expect = 6.366e-7 Identity = 45/169 (26.63%), Postives = 82/169 (48.52%), Query Frame = 2 Query: 242 AALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAK---HSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSD-ICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKL 736 A AL L++ ++ AV+ + +LV L D K AS LRAV Q V++C AL L+ L ++++ + WT+ I N Q+V+DA P+L+ +Q+ + ++ AA A+++ +P+ + +V+ G I L ++ D+K+ Sbjct: 273 ACWALSYLSDGPNEKIQAVIDSGVCRRLVELLMHSDY---KVASPSLRAVGNIVTGDDIQTQVVLNCSALPCLLHLLSSAKESIRKEACWTISNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVNLGCIKPLCDLLTVMDSKI 438
BLAST of Sperm associated antigen 6 vs. Ensembl Xenopus
Match: ufd1 (ubiquitin recognition factor in ER associated degradation 1 [Source:Xenbase;Acc:XB-GENE-963687]) HSP 1 Score: 830.861 bits (2145), Expect = 0.000e+0 Identity = 396/508 (77.95%), Postives = 458/508 (90.16%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPAL 1585 MSQRQVLQVFEQYQ++RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQT+ALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSP+LAQS+VDCGA+D LVICLE+FDP VKE +AW LGYI+RHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSD+ KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKD D+ VKKN +TLIREIA+HTPELSQLIVNAGGVAA++D IG GN+ LPGIMMLGYVAAHSE L+M+VI+S+GV QL+ + + EDHI AA AW+LGQIGRHTPEH++AVA NVLPKLL Y+ D SEDLQ K+KKALK+ILQKC +L ALEPLL DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKK+Q++ + GS L EYIN INNC+PEEIV+YYSPGYS++LLERV+ YQP + Sbjct: 1 MSQRQVLQVFEQYQRARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTSALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPQLAQSIVDCGAVDTLVICLEDFDPGVKESAAWALGYISRHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDLSKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDPDEYVKKNSSTLIREIARHTPELSQLIVNAGGVAAVIDCIGNCSGNVRLPGIMMLGYVAAHSENLAMAVIISKGVPQLAICLSEEAEDHIKAAAAWSLGQIGRHTPEHARAVAVANVLPKLLSLYMDTDSSEDLQVKAKKALKNILQKCTHLPALEPLLHDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKIQEIVADTGSPLQEYINTINNCFPEEIVRYYSPGYSQSLLERVDHYQPVI 508
BLAST of Sperm associated antigen 6 vs. Ensembl Xenopus
Match: ufd1 (ubiquitin recognition factor in ER associated degradation 1 [Source:Xenbase;Acc:XB-GENE-963687]) HSP 1 Score: 798.505 bits (2061), Expect = 0.000e+0 Identity = 382/492 (77.64%), Postives = 442/492 (89.84%), Query Frame = 2 Query: 110 RTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPAL 1585 RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQT+ALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSP+LAQS+VDCGA+D LVICLE+FDP VKE +AW LGYI+RHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSD+ KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKD D+ VKKN +TLIREIA+HTPELSQLIVNAGGVAA++D IG GN+ LPGIMMLGYVAAHSE L+M+VI+S+GV QL+ + + EDHI AA AW+LGQIGRHTPEH++AVA NVLPKLL Y+ D SEDLQ K+KKALK+ILQKC +L ALEPLL DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKK+Q++ + GS L EYIN INNC+PEEIV+YYSPGYS++LLERV+ YQP + Sbjct: 4 RTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTSALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPQLAQSIVDCGAVDTLVICLEDFDPGVKESAAWALGYISRHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDLSKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDPDEYVKKNSSTLIREIARHTPELSQLIVNAGGVAAVIDCIGNCSGNVRLPGIMMLGYVAAHSENLAMAVIISKGVPQLAICLSEEAEDHIKAAAAWSLGQIGRHTPEHARAVAVANVLPKLLSLYMDTDSSEDLQVKAKKALKNILQKCTHLPALEPLLHDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKIQEIVADTGSPLQEYINTINNCFPEEIVRYYSPGYSQSLLERVDHYQPVI 495
BLAST of Sperm associated antigen 6 vs. Ensembl Xenopus
Match: ufd1 (ubiquitin recognition factor in ER associated degradation 1 [Source:Xenbase;Acc:XB-GENE-963687]) HSP 1 Score: 778.089 bits (2008), Expect = 0.000e+0 Identity = 372/478 (77.82%), Postives = 430/478 (89.96%), Query Frame = 2 Query: 89 FEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVK 1522 FEQYQ++RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQT+ALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSP+LAQS+VDCGA+D LVICLE+FDP VKE +AW LGYI+RHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSD+ KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKD D+ VKKN +TLIREIA+HTPELSQLIVNAGGVAA++D IG GN+ LPGIMMLGYVAAHSE L+M+VI+S+GV QL+ + + EDHI AA AW+LGQIGRHTPEH++AVA NVLPKLL Y+ D SEDLQ K+KKALK+ILQKC +L ALEPLL DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKK+Q++ + GS L EYIN INNC+PEEIV+ Sbjct: 13 FEQYQRARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTSALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPQLAQSIVDCGAVDTLVICLEDFDPGVKESAAWALGYISRHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDLSKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDPDEYVKKNSSTLIREIARHTPELSQLIVNAGGVAAVIDCIGNCSGNVRLPGIMMLGYVAAHSENLAMAVIISKGVPQLAICLSEEAEDHIKAAAAWSLGQIGRHTPEHARAVAVANVLPKLLSLYMDTDSSEDLQVKAKKALKNILQKCTHLPALEPLLHDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKIQEIVADTGSPLQEYINTINNCFPEEIVR 490
BLAST of Sperm associated antigen 6 vs. Ensembl Xenopus
Match: ufd1 (ubiquitin recognition factor in ER associated degradation 1 [Source:Xenbase;Acc:XB-GENE-963687]) HSP 1 Score: 776.933 bits (2005), Expect = 0.000e+0 Identity = 373/480 (77.71%), Postives = 430/480 (89.58%), Query Frame = 2 Query: 89 FEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYY 1528 FEQYQ++RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQT+ALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSP+LAQS+VDCGA+D LVICLE+FDP VKE +AW LGYI+RHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSD+ KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKD D+ VKKN +TLIREIA+HTPELSQLIVNAGGVAA++D IG GN+ LPGIMMLGYVAAHSE L+M+VI+S+GV QL+ + + EDHI AA AW+LGQIGRHTPEH++AVA NVLPKLL Y+ D SEDLQ K+KKALK+ILQKC +L ALEPLL DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKK+Q++ + GS L EYIN INNC+PEEIV Y Sbjct: 13 FEQYQRARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTSALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPQLAQSIVDCGAVDTLVICLEDFDPGVKESAAWALGYISRHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDLSKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDPDEYVKKNSSTLIREIARHTPELSQLIVNAGGVAAVIDCIGNCSGNVRLPGIMMLGYVAAHSENLAMAVIISKGVPQLAICLSEEAEDHIKAAAAWSLGQIGRHTPEHARAVAVANVLPKLLSLYMDTDSSEDLQVKAKKALKNILQKCTHLPALEPLLHDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKIQEIVADTGSPLQEYINTINNCFPEEIVSPY 492
BLAST of Sperm associated antigen 6 vs. Ensembl Xenopus
Match: ufd1 (ubiquitin recognition factor in ER associated degradation 1 [Source:Xenbase;Acc:XB-GENE-963687]) HSP 1 Score: 775.778 bits (2002), Expect = 0.000e+0 Identity = 373/480 (77.71%), Postives = 430/480 (89.58%), Query Frame = 2 Query: 89 FEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYY 1528 FEQYQ++RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQT+ALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSP+LAQS+VDCGA+D LVICLE+FDP VKE +AW LGYI+RHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSD+ KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKD D+ VKKN +TLIREIA+HTPELSQLIVNAGGVAA++D IG GN+ LPGIMMLGYVAAHSE L+M+VI+S+GV QL+ + + EDHI AA AW+LGQIGRHTPEH++AVA NVLPKLL Y+ D SEDLQ K+KKALK+ILQKC +L ALEPLL DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKK+Q++ + GS L EYIN INNC+PEEIV Y Sbjct: 10 FEQYQRARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTSALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPQLAQSIVDCGAVDTLVICLEDFDPGVKESAAWALGYISRHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDLSKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDPDEYVKKNSSTLIREIARHTPELSQLIVNAGGVAAVIDCIGNCSGNVRLPGIMMLGYVAAHSENLAMAVIISKGVPQLAICLSEEAEDHIKAAAAWSLGQIGRHTPEHARAVAVANVLPKLLSLYMDTDSSEDLQVKAKKALKNILQKCTHLPALEPLLHDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKIQEIVADTGSPLQEYINTINNCFPEEIVSPY 489
BLAST of Sperm associated antigen 6 vs. Ensembl Mouse
Match: Spag6l (sperm associated antigen 6-like [Source:MGI Symbol;Acc:MGI:1354388]) HSP 1 Score: 841.262 bits (2172), Expect = 0.000e+0 Identity = 404/506 (79.84%), Postives = 455/506 (89.92%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQ +AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW LGYIARHN +L+Q+VVDAGA+PLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN TLIREIAKHTPELSQLIVNAGGVAA++D IG KGNI LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQ+GRHTPEH++AVA N LP LL Y+ P+ SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGS L EYIN INNCYPEEIV+YYSPGYS+ LL+RV+SYQP Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALGYIARHNTELSQAVVDAGAIPLLVLCIQEPEIALKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNACTLIREIAKHTPELSQLIVNAGGVAAVIDCIGSCKGNIRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSICLSEEPEDHIKAAAAWALGQLGRHTPEHARAVAVTNTLPVLLSLYMSPESSEDLQLKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINNCYPEEIVRYYSPGYSDTLLQRVDSYQP 506
BLAST of Sperm associated antigen 6 vs. Ensembl Mouse
Match: Spag6 (sperm associated antigen 6 [Source:MGI Symbol;Acc:MGI:3040687]) HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0 Identity = 388/506 (76.68%), Postives = 446/506 (88.14%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFE YQ +RT+FVQ +AE A +PQNIETLQ AG+M+LLRPLLLDVVP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+ YKKAA+FVLRAV KHSP+LAQ+ VDCGALD+LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP+ LKRIAASALSDI KHSP+LAQTVVD GAIAHLAQM+LNPD KLK+Q+ SAL+ IAKHSVDLAE+VVEAEIFP +LTCLKDKDD VKKN TLIREIAKHTPELSQLIVNAGGVAA++D IG KGNI LPGIMMLGY+AAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQ+GRHTPEH++AVA N LP LL Y+ P+ SEDLQ KSKKA+K+I+QKC YL ALEP L DAPPNILK+V QFSKVLP++ +ARRLFVTSGGLKK+Q++K EPGS L EYIN IN+CYPEEIV+YYSPGYS+ LL+R++SYQP Sbjct: 1 MSQRQVLQVFELYQNARTRFVQMVAEQATKPQNIETLQNAGIMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNCVYKKAAAFVLRAVGKHSPQLAQATVDCGALDSLVICLEDFDPGVKEAAAWALAYIARHNAELSQAVVDAGAVPLLVLCIQEPETALKRIAASALSDISKHSPELAQTVVDVGAIAHLAQMILNPDEKLKQQVLSALSHIAKHSVDLAEMVVEAEIFPVVLTCLKDKDDFVKKNACTLIREIAKHTPELSQLIVNAGGVAAVIDCIGSCKGNIRLPGIMMLGYMAAHSENLAMAVIISKGVPQLSDCLSEEPEDHIKAAAAWALGQVGRHTPEHARAVAITNTLPVLLALYMSPESSEDLQVKSKKAIKNIIQKCTYLPALEPFLYDAPPNILKYVAGQFSKVLPHDSKARRLFVTSGGLKKIQEIKAEPGSILQEYINNINSCYPEEIVRYYSPGYSDILLQRIDSYQP 506
BLAST of Sperm associated antigen 6 vs. Ensembl Mouse
Match: Spag6 (sperm associated antigen 6 [Source:MGI Symbol;Acc:MGI:3040687]) HSP 1 Score: 256.144 bits (653), Expect = 9.216e-83 Identity = 133/166 (80.12%), Postives = 152/166 (91.57%), Query Frame = 2 Query: 131 IAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAAS 628 +AE A +PQNIETLQ AG+M+LLRPLLLDVVP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+ YKKAA+FVLRAV KHSP+LAQ+ VDCGALD+LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP+ LKRIAAS Sbjct: 2 VAEQATKPQNIETLQNAGIMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNCVYKKAAAFVLRAVGKHSPQLAQATVDCGALDSLVICLEDFDPGVKEAAAWALAYIARHNAELSQAVVDAGAVPLLVLCIQEPETALKRIAAS 167 HSP 2 Score: 72.4034 bits (176), Expect = 1.810e-14 Identity = 50/159 (31.45%), Postives = 89/159 (55.97%), Query Frame = 2 Query: 404 PELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCAT 880 P+ +++ + G + L L + P +++ +A LG +A +N LA++VV +P LV + E + K+ AA L + KHSP LAQ VD GA+ L + + D +K ALA IA+H+ +L++ VV+A P ++ C+++ + +K+ A+ Sbjct: 9 PQNIETLQNAGIMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNCVYKKAAAFVLRAVGKHSPQLAQATVDCGALDSLVICLEDFDPGVKEAAAWALAYIARHNAELSQAVVDAGAVPLLVLCIQEPETALKRIAAS 167 HSP 3 Score: 55.8398 bits (133), Expect = 1.019e-8 Identity = 30/101 (29.70%), Postives = 63/101 (62.38%), Query Frame = 2 Query: 656 PDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIV 958 P +T+ +AG ++ L ++L+ +++ AL ++A ++ DLAE VV+ +I P ++ L +++ + KK A ++R + KH+P+L+Q V+ G + ++V Sbjct: 9 PQNIETLQNAGIMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNCVYKKAAAFVLRAVGKHSPQLAQATVDCGALDSLV 109 HSP 4 Score: 53.5286 bits (127), Expect = 6.071e-8 Identity = 39/122 (31.97%), Postives = 63/122 (51.64%), Query Frame = 2 Query: 608 LKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGE 973 +++ AA AL + ++ DLA+ VV + L + + K+ L + KHS LA+ V+ +++ CL+D D VK+ A + IA+H ELSQ +V+AG V +V I E Sbjct: 35 IQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNCVYKKAAAFVLRAVGKHSPQLAQATVDCGALDSLVICLEDFDPGVKEAAAWALAYIARHNAELSQAVVDAGAVPLLVLCIQE 156
BLAST of Sperm associated antigen 6 vs. Ensembl Mouse
Match: Kpna7 (karyopherin alpha 7 (importin alpha 8) [Source:MGI Symbol;Acc:MGI:2141165]) HSP 1 Score: 61.2326 bits (147), Expect = 1.767e-9 Identity = 58/247 (23.48%), Postives = 111/247 (44.94%), Query Frame = 2 Query: 296 VVKGDILPQLVYSL-AEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQE--PDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAA 1027 +++ ++P+LV L A + A++VL +A + E ++VV GA+ L+ L V E + W LG IA A+ V+ A+P L+ + + P L+ I+ + LS++C++ + L Q++L+ D ++ AL+ + K + VV I P ++ + + + C I I T E +Q+ ++AG + + + K +I + + VAA Sbjct: 107 IIEAGLIPKLVDFLKATPHPKLQFEAAWVLTNIASGTSEQTRAVVKEGAIQPLIELLCSPHLTVSEQAVWALGNIAGDCAEFRDCVISNNAIPHLINLISKGIPITFLRNISWT-LSNLCRNKDPYPSESAVRQMLPPLCQLLLHRDNEILADTCWALSYLTKGGKEYIHHVVTTGILPRLVELMTSSELSISIPCLHTIGNIVAGTDEQTQMAIDAGMLKVLGQVLKHPKTSIQVLAAWTMSNVAA 352 HSP 2 Score: 53.1434 bits (126), Expect = 6.505e-7 Identity = 56/267 (20.97%), Postives = 114/267 (42.70%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEF-DPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLN--PDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 +++ G + LV L+ P ++ +AW L IA ++ ++VV GA+ L+ + P L + A AL +I + V+ AI HL ++ P T L R I L+ + ++ ++ P + L +D+ + + + + K E +V G + +V+ + ++ +IS+P + +G + A ++ + + G+L++ + I AW + + P H N+LP L+ Sbjct: 107 IIEAGLIPKLVDFLKATPHPKLQFEAAWVLTNIASGTSEQTRAVVKEGAIQPLIELLCSPHLTVSEQAVWALGNIAGDCAEFRDCVISNNAIPHLINLISKGIPITFL-RNISWTLSNLCRNKDPYPSESAVRQMLPPLCQLLLHRDNEILADTCWALSYLTKGGKEYIHHVVTTGILPRLVELMTSSELSISIPCLHTIGNIVAGTDE-QTQMAIDAGMLKVLGQVLKHPKTSIQVLAAWTMSNVAA-GPRHQVEQLLCNLLPILV 370
BLAST of Sperm associated antigen 6 vs. Ensembl Mouse
Match: Kpna7 (karyopherin alpha 7 (importin alpha 8) [Source:MGI Symbol;Acc:MGI:2141165]) HSP 1 Score: 61.2326 bits (147), Expect = 1.767e-9 Identity = 58/247 (23.48%), Postives = 111/247 (44.94%), Query Frame = 2 Query: 296 VVKGDILPQLVYSL-AEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQE--PDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAA 1027 +++ ++P+LV L A + A++VL +A + E ++VV GA+ L+ L V E + W LG IA A+ V+ A+P L+ + + P L+ I+ + LS++C++ + L Q++L+ D ++ AL+ + K + VV I P ++ + + + C I I T E +Q+ ++AG + + + K +I + + VAA Sbjct: 107 IIEAGLIPKLVDFLKATPHPKLQFEAAWVLTNIASGTSEQTRAVVKEGAIQPLIELLCSPHLTVSEQAVWALGNIAGDCAEFRDCVISNNAIPHLINLISKGIPITFLRNISWT-LSNLCRNKDPYPSESAVRQMLPPLCQLLLHRDNEILADTCWALSYLTKGGKEYIHHVVTTGILPRLVELMTSSELSISIPCLHTIGNIVAGTDEQTQMAIDAGMLKVLGQVLKHPKTSIQVLAAWTMSNVAA 352 HSP 2 Score: 53.1434 bits (126), Expect = 6.505e-7 Identity = 56/267 (20.97%), Postives = 114/267 (42.70%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEF-DPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLN--PDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 +++ G + LV L+ P ++ +AW L IA ++ ++VV GA+ L+ + P L + A AL +I + V+ AI HL ++ P T L R I L+ + ++ ++ P + L +D+ + + + + K E +V G + +V+ + ++ +IS+P + +G + A ++ + + G+L++ + I AW + + P H N+LP L+ Sbjct: 107 IIEAGLIPKLVDFLKATPHPKLQFEAAWVLTNIASGTSEQTRAVVKEGAIQPLIELLCSPHLTVSEQAVWALGNIAGDCAEFRDCVISNNAIPHLINLISKGIPITFL-RNISWTLSNLCRNKDPYPSESAVRQMLPPLCQLLLHRDNEILADTCWALSYLTKGGKEYIHHVVTTGILPRLVELMTSSELSISIPCLHTIGNIVAGTDE-QTQMAIDAGMLKVLGQVLKHPKTSIQVLAAWTMSNVAA-GPRHQVEQLLCNLLPILV 370
BLAST of Sperm associated antigen 6 vs. UniProt/SwissProt
Match: sp|Q9JLI7|SPAG6_MOUSE (Sperm-associated antigen 6 OS=Mus musculus OX=10090 GN=Spag6 PE=1 SV=1) HSP 1 Score: 841.262 bits (2172), Expect = 0.000e+0 Identity = 404/506 (79.84%), Postives = 455/506 (89.92%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQ +AELA RPQNIETLQ AGVM+LLRPLLLDVVP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW LGYIARHN +L+Q+VVDAGA+PLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+ SAL+QIAKHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN TLIREIAKHTPELSQLIVNAGGVAA++D IG KGNI LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQ+GRHTPEH++AVA N LP LL Y+ P+ SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGS L EYIN INNCYPEEIV+YYSPGYS+ LL+RV+SYQP Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALGYIARHNTELSQAVVDAGAIPLLVLCIQEPEIALKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNACTLIREIAKHTPELSQLIVNAGGVAAVIDCIGSCKGNIRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSICLSEEPEDHIKAAAAWALGQLGRHTPEHARAVAVTNTLPVLLSLYMSPESSEDLQLKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINNCYPEEIVRYYSPGYSDTLLQRVDSYQP 506
BLAST of Sperm associated antigen 6 vs. UniProt/SwissProt
Match: sp|O75602|SPAG6_HUMAN (Sperm-associated antigen 6 OS=Homo sapiens OX=9606 GN=SPAG6 PE=2 SV=1) HSP 1 Score: 825.083 bits (2130), Expect = 0.000e+0 Identity = 397/506 (78.46%), Postives = 451/506 (89.13%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQ +AELA RPQNIETLQ AGVM+LLR LLLDVVP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAV KHSP+LAQ++VDCGALD LVICLE+FDP VKE +AW L YIARHNA+L+Q+VVDAGAVPLLVLC+QEP++ LKRIAASALSDI KHSP+LAQTVVDAGA+AHLAQM+LNPD KLK QI SAL+Q++KHSVDLAE+VVEAEIFP +LTCLKDKD+ VKKN +TLIREIAKHTPELSQL+VNAGGVAA++D IG KGN LPGIMMLGYVAAHSE L+M+VI+S+GV QLS + + EDHI AA AWALGQIGRHTPEH++AVA N LP LL Y+ + SEDLQ KSKKA+K+ILQKC YL ALEP L DAPPNILKHVV QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGS L EYIN IN+CYPEEIV+YYSPGYS+ LL+RV+SYQP Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAKHTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPEDHIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVRYYSPGYSDTLLQRVDSYQP 506
BLAST of Sperm associated antigen 6 vs. UniProt/SwissProt
Match: sp|G5EB89|KAPA_EMENI (Importin subunit alpha OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=kapA PE=1 SV=1) HSP 1 Score: 66.6254 bits (161), Expect = 3.113e-10 Identity = 72/322 (22.36%), Postives = 136/322 (42.24%), Query Frame = 2 Query: 266 ANHSDDLALAVVKGDI---LPQLVYS-LAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 A SDD A A+ + ++ LP++V ++Q +A + + ++K + V++ G + V L V+ +AW L IA +AQ Q V++AGAVP+ V + P+ ++ A AL +I SP V++AGA+ L ++ N K+ R L+ + + A P + + DD V + I ++ E Q ++ AG +V+ + ++ P + +G + + + VI++ G L + +D I W + I ++V ++P L+ Sbjct: 66 AAESDDEASAI-ESELNVELPEMVKGVFSDQIEAQIQATTKFRKLLSKERNPPIERVIETGVVSRFVEFLRSPHTLVQFEAAWALTNIASGSAQQTQVVIEAGAVPIFVELLSSPEPDVREQAVWALGNIAGDSPQCRDFVLNAGALRPLLTLI-NDGRKISMLRNATWTLSNFCRGKTPQPDWNTIAPALPVLAKLIYMLDDEVLIDACWAISYLSDGPNEKIQAVIEAGIPRRLVELLMHASTSVQTPALRSVGNIVT-GDDVQTQVIINCGALPALLSLLSSTKDGIRKEACWTISNITAGNSSQIQSVIDAGIIPPLV 384 HSP 2 Score: 55.0694 bits (131), Expect = 1.149e-6 Identity = 47/181 (25.97%), Postives = 81/181 (44.75%), Query Frame = 2 Query: 242 AALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICK---HSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAK 775 A A+ L++ ++ AV++ I +LV L + A + + Q +++CGAL AL+ L +++ + WT+ I N+ QSV+DAG +P LV + D ++ A A+S+ PD + +V G I L ++ PD K+ + L I K Sbjct: 272 ACWAISYLSDGPNEKIQAVIEAGIPRRLVELLMHASTSVQTPALRSVGNIVTGDDVQTQVIINCGALPALLSLLSSTKDGIRKEACWTISNITAGNSSQIQSVIDAGIIPPLVHLLANGDFKTRKEACWAISNATSGGLQKPDQIRYLVTQGCIKPLCDLLACPDNKIIQVALDGLENILK 452
BLAST of Sperm associated antigen 6 vs. UniProt/SwissProt
Match: sp|B9DHT4|ARIA_ARATH (ARM REPEAT PROTEIN INTERACTING WITH ABF2 OS=Arabidopsis thaliana OX=3702 GN=ARIA PE=1 SV=2) HSP 1 Score: 65.855 bits (159), Expect = 5.924e-10 Identity = 64/270 (23.70%), Postives = 122/270 (45.19%), Query Frame = 2 Query: 329 YSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEE------------FDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLG--------LKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQ 1078 +S E DR K A+ VL +AK+ +L +VD GA+ AL+ L+ ++ V++GSA+ LG +A + + +VD GA+P LV ++ G + R AA A++++ + + V G I L +++ D+K++R AL +A + D +VE P ++ L +D + +I + +P + + ++ AG + ++ + ++LG A+ + IV RG ++ Sbjct: 61 FSWQESDRAAAKRATQVLAELAKNE-DLVNVIVDGGAVPALMTHLQAPPYNDGDLAEKPYEHEVEKGSAFALGLLA-IKPEYQKLIVDKGALPHLVNLLKRNKDGSSSRAVNSVIRRAADAITNLAHENSSIKTRVRVEGGIPPLVELLEFSDSKVQRAAAGALRTLAFKNDDNKNQIVECNALPTLILMLGSEDAAIHYEAVGVIGNLVHSSPHIKKEVLTAGALQPVIGLLSSCCPESQREAALLLGQFASTDSDCKVH-IVQRGAVR 327 HSP 2 Score: 58.9214 bits (141), Expect = 8.506e-8 Identity = 57/191 (29.84%), Postives = 89/191 (46.60%), Query Frame = 2 Query: 131 IAELANRPQNIET-LQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAH 700 I LA+ +I+T ++ G + L LL +Q+ AA AL LA +DD +V+ + LP L+ L +D A V+ + SP + + V+ GAL ++ L P + +A LG A ++ +V GAV L+ +Q PD+ LK ++A AL LAQ + IAH Sbjct: 180 ITNLAHENSSIKTRVRVEGGIPPLVELLEFSDSKVQRAAAGALRTLAFKNDDNKNQIVECNALPTLILMLGSEDAAIHYEAVGVIGNLVHSSPHIKKEVLTAGALQPVIGLLSSCCPESQREAALLLGQFASTDSDCKVHIVQRGAVRPLIEMLQSPDVQLKEMSAFALG-------RLAQDAHNQAGIAH 363
BLAST of Sperm associated antigen 6 vs. UniProt/SwissProt
Match: sp|B6HJ92|KAPA_PENRW (Importin subunit alpha OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=kapA PE=1 SV=1) HSP 1 Score: 63.929 bits (154), Expect = 2.117e-9 Identity = 75/334 (22.46%), Postives = 139/334 (41.62%), Query Frame = 2 Query: 236 QTAALALGRLA--NHSDDLALAVVKGDI---LPQLVYS-LAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 Q + +G +A SDD A A+ + ++ LPQ+V ++Q +A + + ++K + V++ G + V L V+ SAW L IA +AQ Q V++AGAVP+ V + P+ ++ A AL +I SP V+ AGA+ L ++ N KL R L+ + + A P + + DD V + I ++ + Q ++ AG +V+ + ++ P + +G + + + VI++ G L + +D I W + + +AV ++ L+ Sbjct: 54 QDGRIGVGGMAAATESDDEASAI-ESELNVELPQMVKGVFSDQVEEQIQATTKFRKLLSKERNPPIERVIETGVVARFVEFLRSPHTLVQFESAWALTNIASGSAQQTQVVIEAGAVPIFVELLSSPEPDVREQAVWALGNIAGDSPHCRDFVLGAGALRPLLNLI-NDGRKLSMLRNATWTLSNFCRGKTPQPDWNTIAPALPVLAKLIYMLDDEVLIDACWAISYLSDGANDKIQAVIEAGIPRRLVELLMHASTSVQTPALRSVGNIVT-GDDVQTQVIINCGSLPALLSLLSSTKDGIRKEACWTISNVTAGNSSQIQAVIDAGIIAPLI 384
BLAST of Sperm associated antigen 6 vs. TrEMBL
Match: A0A1I8HT46 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024813g1 PE=4 SV=1) HSP 1 Score: 897.501 bits (2318), Expect = 0.000e+0 Identity = 427/506 (84.39%), Postives = 478/506 (94.47%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLD+VP+IQQTAALALGRLAN++D+LA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSPELAQ+VVDCGALDALVICLEEFDP VKE +AW LGYIARH+A+LAQSVVDAGAVPLLVLCVQEPDL LKRIAASALSDI KHSP+LAQTVVDAGAIAHLAQMVLNPD KLKRQ+FSALAQIAKHSVDLAE+VVEAEIFPA+LTCLKD D+ V+KNCATLIRE+AKHTPELSQLIVNAGGVAA+VDF+G+++GN+ LPG+MMLGYVAAHSE L+M++IVSRGVL L ++ + EDHI AA AW+LGQIGRH+PEH+K+VA+ NVLPKLLR YLRPDVSEDLQ KSKKALKHILQKCV+LEALEPLLQ+APPNILKHVVAQFSKVLP+NP++RRLFVTSGGLKK+Q+++ EPGSAL+EYIN INNCYPEEIVKYYSPGYS+ALLER+E Y P Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDIVPTIQQTAALALGRLANYNDELAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPELAQAVVDCGALDALVICLEEFDPGVKESAAWALGYIARHSAELAQSVVDAGAVPLLVLCVQEPDLSLKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMVLNPDAKLKRQVFSALAQIAKHSVDLAEMVVEAEIFPAVLTCLKDPDEYVRKNCATLIREVAKHTPELSQLIVNAGGVAAMVDFVGDSRGNVRLPGVMMLGYVAAHSENLAMAIIVSRGVLGLDAVLQTEPEDHIQAAAAWSLGQIGRHSPEHAKSVAQANVLPKLLRAYLRPDVSEDLQIKSKKALKHILQKCVHLEALEPLLQEAPPNILKHVVAQFSKVLPHNPKSRRLFVTSGGLKKIQEIRAEPGSALAEYINTINNCYPEEIVKYYSPGYSDALLERIEHYLP 506
BLAST of Sperm associated antigen 6 vs. TrEMBL
Match: A0A1I8GNF6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003556g1 PE=4 SV=1) HSP 1 Score: 896.345 bits (2315), Expect = 0.000e+0 Identity = 426/506 (84.19%), Postives = 479/506 (94.66%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLD+VP+IQQTAALALGRLAN++D+LA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSPELAQ+VVDCGALDALVICLEEFDP VKE +AW LGYIARH+A+LAQSVVDAGAVPLLVLCVQEPDL LKRIAASALSDI KHSP+LAQTVVDAGAIAHLAQMVLNPD KLKRQ+FSALAQ+AKHSVDLAE+VVEAEIFPA+LTCLKD D+ V+KNCATLIRE+AKHTPELSQLIVNAGGVAA+VDF+G+++GN+ LPG+MMLGYVAAHSE L+M++IVSRGVL L ++ + EDHI AA AW+LGQIGRH+PEH+K+VA+ NVLPKLLR YLRPDVSEDLQ KSKKALKHILQKCV+LEALEPLLQ+APPNILKHVVAQFSKVLP+NP++RRLFVTSGGLKK+Q+++ EPGSAL+EYIN INNCYPEEIVKYYSPGYS+ALLER+E+Y P Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDIVPTIQQTAALALGRLANYNDELAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPELAQAVVDCGALDALVICLEEFDPGVKESAAWALGYIARHSAELAQSVVDAGAVPLLVLCVQEPDLSLKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMVLNPDAKLKRQVFSALAQMAKHSVDLAEMVVEAEIFPAVLTCLKDPDEYVRKNCATLIREVAKHTPELSQLIVNAGGVAAMVDFVGDSRGNVRLPGVMMLGYVAAHSENLAMAIIVSRGVLGLDAVLQTEPEDHIQAAAAWSLGQIGRHSPEHAKSVAQANVLPKLLRAYLRPDVSEDLQIKSKKALKHILQKCVHLEALEPLLQEAPPNILKHVVAQFSKVLPHNPKSRRLFVTSGGLKKIQEIRAEPGSALAEYINTINNCYPEEIVKYYSPGYSDALLERIENYLP 506
BLAST of Sperm associated antigen 6 vs. TrEMBL
Match: A0A267DQ56 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008678g1 PE=4 SV=1) HSP 1 Score: 894.804 bits (2311), Expect = 0.000e+0 Identity = 427/506 (84.39%), Postives = 475/506 (93.87%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQVLQVFEQYQK+RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLD+VP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSPELAQ+VVDCGALDALVICLEEFDP VKE +AW LGYIARH +LAQ+VVDAGAVPLLVLCVQEPDL LKRIAASALSDI KHSP+LAQTVVDAGAIAHLAQMVLNPD KLKRQ+FSALAQI+KHSVDLAE+VVEAEIFPA+LTCLKD D+ V+KNCATLIRE+AKHTPELSQLIVNAGGVAAIVDF+G+++GN+ LPG+MMLGYVAAHSE L+M+VIVSRGVL L ++ + EDHI AA AW+LGQIGRH+PEH+KAVA+ NVLPKLLR YLRPDVSEDLQ KSKKALKHILQKCV+LEALEPLLQ+AP NILKHVVAQFSKVLP+NP++RRLFVTSGGLKK+Q+++ EPGSAL+EYIN INNCYPEEIVKYYSPGYS++LLERVE Y P Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDIVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPELAQAVVDCGALDALVICLEEFDPGVKESAAWALGYIARHGPELAQAVVDAGAVPLLVLCVQEPDLSLKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMVLNPDAKLKRQVFSALAQISKHSVDLAEMVVEAEIFPAVLTCLKDPDEYVRKNCATLIREVAKHTPELSQLIVNAGGVAAIVDFVGDSRGNVRLPGVMMLGYVAAHSENLAMAVIVSRGVLTLDAVLQTESEDHIQAAAAWSLGQIGRHSPEHAKAVAQANVLPKLLRAYLRPDVSEDLQIKSKKALKHILQKCVHLEALEPLLQEAPANILKHVVAQFSKVLPHNPKSRRLFVTSGGLKKIQEIRAEPGSALAEYINTINNCYPEEIVKYYSPGYSDSLLERVEHYLP 506
BLAST of Sperm associated antigen 6 vs. TrEMBL
Match: A0A1I8HVJ4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 887.1 bits (2291), Expect = 0.000e+0 Identity = 423/500 (84.60%), Postives = 471/500 (94.20%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLER 1561 MSQRQVLQVFEQYQK+RTQFVQT+AELA RPQNIETLQ AGVM+LLRPLLLD+VP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSPELAQ+VVDCGALDALVICLEEFDP VKE +AW LGYIARH +LAQ+VVDAGAVPLLVLCVQEPDL LKRIAASALSDI KHSP+LAQTVVDAGAIAHLAQMVLNPD KLKRQ+FSALAQI+KHSVDLAE+VVEAEIFPA+LTCLKD D+ V+KNCATLIRE+AKHTPELSQLIVNAGGVAAIVDF+G+++GN+ LPG+MMLGYVAAHSE L+M+VIVSRGVL L ++ + EDHI AA AW+LGQIGRH+PEH+KAVA+ NVLPKLLR YLRPDVSEDLQ KSKKALKHILQKCV+LEALEPLLQ+AP NILKHVVAQFSKVLP+NP++RRLFVTSGGLKK+Q+++ EPGSAL+EYIN INNCYPEEIVKYYSPGYS++LLER Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQTVAELATRPQNIETLQNAGVMSLLRPLLLDIVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPELAQAVVDCGALDALVICLEEFDPGVKESAAWALGYIARHGPELAQAVVDAGAVPLLVLCVQEPDLSLKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMVLNPDAKLKRQVFSALAQISKHSVDLAEMVVEAEIFPAVLTCLKDPDEYVRKNCATLIREVAKHTPELSQLIVNAGGVAAIVDFVGDSRGNVRLPGVMMLGYVAAHSENLAMAVIVSRGVLTLDAVLQTESEDHIQAAAAWSLGQIGRHSPEHAKAVAQANVLPKLLRAYLRPDVSEDLQIKSKKALKHILQKCVHLEALEPLLQEAPANILKHVVAQFSKVLPHNPKSRRLFVTSGGLKKIQEIRAEPGSALAEYINTINNCYPEEIVKYYSPGYSDSLLER 500
BLAST of Sperm associated antigen 6 vs. TrEMBL
Match: K1PQU8 (Sperm-associated antigen 6 OS=Crassostrea gigas OX=29159 GN=CGI_10011025 PE=4 SV=1) HSP 1 Score: 883.248 bits (2281), Expect = 0.000e+0 Identity = 424/507 (83.63%), Postives = 473/507 (93.29%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPA 1582 MSQRQVLQVFEQYQK+RT FVQT+AELA+RPQNIETLQ AGVM+LLRPLLLD+VP+IQQTAALALGRLAN++DDLA AVVKGDILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHSP+LAQSVVDCGALDALVICLEEFDP VKE +AW LGYIARHNA+LAQ+VVDAGAVPLLVLC+QEP+L LKRIAASALSDICKHSP+LAQTVVDAGAIAHLAQM+LNPD KLKRQ+FSAL+QI+KHSVDLAE+VVEAEIFPA+LTCLKD D+ VKKN ATLIREIAKHTPEL+QLIVNAGGVAA+VD++GE+KGNI LPGIMMLGYVAAHSE L+M+VIVS+GV+QL+ + + EDHI AA AWALGQIGRHTPEH+KAVA NVLPKLL+CYLR D SEDLQ KSKKALK+ILQKCV+L ALEPLL DAPPNILKHVV QFSKVLP++ +ARRLFVTS GLKK+Q++K EPGSAL+EYIN INNCYPEEIVKYYSPGYS+ LLERVE YQP+ Sbjct: 1 MSQRQVLQVFEQYQKARTGFVQTVAELASRPQNIETLQNAGVMSLLRPLLLDIVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSPQLAQSVVDCGALDALVICLEEFDPGVKESAAWALGYIARHNAELAQAVVDAGAVPLLVLCIQEPELSLKRIAASALSDICKHSPELAQTVVDAGAIAHLAQMILNPDAKLKRQVFSALSQISKHSVDLAEMVVEAEIFPAVLTCLKDPDEYVKKNVATLIREIAKHTPELAQLIVNAGGVAAVVDYVGESKGNIRLPGIMMLGYVAAHSENLAMAVIVSKGVVQLAIALSEEPEDHIQAAAAWALGQIGRHTPEHAKAVAVANVLPKLLQCYLRADASEDLQTKSKKALKNILQKCVHLPALEPLLHDAPPNILKHVVGQFSKVLPHDSKARRLFVTSAGLKKIQEIKAEPGSALAEYINTINNCYPEEIVKYYSPGYSDTLLERVEQYQPS 507
BLAST of Sperm associated antigen 6 vs. Ensembl Cavefish
Match: spag6 (sperm associated antigen 6 [Source:NCBI gene;Acc:103022863]) HSP 1 Score: 828.55 bits (2139), Expect = 0.000e+0 Identity = 394/507 (77.71%), Postives = 454/507 (89.55%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPA 1582 MSQRQVLQVFEQYQK+RTQFVQT+A+LA RPQNIETLQ AGVM+LLRPLLLD VP++QQTAALALGRLAN++DDLA A+VK DILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHS ELAQ+VVDCGALDALV+ +EEFDP VKE +AW LGYIARHNAQLAQ+VVDAGAVPLLVLC+QEP++ LKRIAAS LSDI KHSP+LAQTVVD GAIAHLAQM LNPD KLKRQ+FSAL QI+KHSVDLAE+VVEAEIFPA+L+ LKD D+ VKKN T++REI KHTPELSQ+IVNAGGVAA++D++G+++GN+ LPGIMMLGY+AAHSE L+M+VIVS+GV QL+ + EDHI AATAWA GQIG+HTPEH++AVA NVLPKLL YL SEDLQ K+KKALK+ILQKC YL ALEPLL +AP NILKHV+ QFSKVLP++ +ARRLFVTSGGLKKVQ++K EPGSAL E+IN INNCYPEEIV+YYSPGYSE LLERV++YQP+ Sbjct: 1 MSQRQVLQVFEQYQKARTQFVQTVADLATRPQNIETLQNAGVMSLLRPLLLDAVPTVQQTAALALGRLANYNDDLADAIVKADILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSAELAQAVVDCGALDALVVSVEEFDPGVKEAAAWALGYIARHNAQLAQAVVDAGAVPLLVLCIQEPEIALKRIAASTLSDIAKHSPELAQTVVDTGAIAHLAQMTLNPDAKLKRQVFSALGQISKHSVDLAEMVVEAEIFPAVLSSLKDPDEYVKKNVTTMMREITKHTPELSQMIVNAGGVAAVIDYLGDSRGNVRLPGIMMLGYIAAHSENLAMAVIVSKGVPQLAICMAEESEDHIKAATAWAFGQIGQHTPEHARAVAAANVLPKLLNLYLDTQSSEDLQVKAKKALKNILQKCTYLPALEPLLYEAPSNILKHVICQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSALQEHINAINNCYPEEIVRYYSPGYSEVLLERVDNYQPS 507
BLAST of Sperm associated antigen 6 vs. Ensembl Cavefish
Match: kpna5 (karyopherin alpha 5 (importin alpha 6) [Source:NCBI gene;Acc:553205]) HSP 1 Score: 61.6178 bits (148), Expect = 1.068e-9 Identity = 61/267 (22.85%), Postives = 114/267 (42.70%), Query Frame = 2 Query: 179 AGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYK-KAASFVLRAV--AKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIRE-IAKHTPELSQLIVNAGGVAAIVDFI 967 G + + LL +Q+ A ALG +A + + V+ ILP L LA+ +R + A + L + K+ P V C L L L DP V + W L Y++ Q+V+D+G LV + D + A A+ +I Q +++ A+ L ++ +P +K++ ++ I + + V++A IFP ++ L+ + +K A I + +PE + +V+ + + D + Sbjct: 173 TGAVPIFIELLNSEYEDVQEQAVWALGNIAGDNAECRDFVLNCGILPSLQQLLAKSNRLTTTRNAVWALSNLCRGKNPPPDFSKVSPC--LSVLSRLLFSSDPDVLADACWALSYLSDGPNDKIQAVIDSGVCRRLVELLMHSDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIKKEACWTVSNITAGNRAQIQTVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGSPEQIRYLVSLNCIKPMCDLL 437
BLAST of Sperm associated antigen 6 vs. Ensembl Cavefish
Match: kpna7 (importin subunit alpha-5-like [Source:NCBI gene;Acc:103041448]) HSP 1 Score: 60.8474 bits (146), Expect = 1.803e-9 Identity = 56/255 (21.96%), Postives = 106/255 (41.57%), Query Frame = 2 Query: 395 KHSPELAQSVVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAI-AHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIG 1153 + +P L + VV+ G L V L D P ++ +AW+L +A + Q VV+ GAVP + + P L + A AL +I P ++D I A LA++ + R + L+ + ++ ++ P+I+ L D V + I + E +I+ G + +V+ + + + P + +G + + S+ + + I GVL + ++ AWA+ I Sbjct: 104 ERNPPLKE-VVEAGLLSRFVEFLSRNDNPTLQFEAAWSLTNVASGTSWHTQQVVEHGAVPAFISLLASPMLNISEQAVWALGNIAGDGPSYRDALIDCNVIPALLARLTPDAPVGYLRNLTWTLSNLCRNKNPFPRFSAVQQMLPSIIQLLHHSDKSVLSDACWAISYLTDGPNERIDVIIKTGILPRLVELLTFEELAVVTPALRSIGNIVSGSDVQTQAAI-DAGVLTALPKLMRHPKPNVQKEAAWAVSNIA 356
BLAST of Sperm associated antigen 6 vs. Ensembl Cavefish
Match: kpna3 (karyopherin subunit alpha 3 [Source:NCBI gene;Acc:103046682]) HSP 1 Score: 60.8474 bits (146), Expect = 1.841e-9 Identity = 58/295 (19.66%), Postives = 120/295 (40.68%), Query Frame = 2 Query: 143 ANRPQNIETLQCAGVMNLL-RPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALV------------------------------------------ICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA 898 ++R I+ L +G++ +L + L D PS+Q AA AL +A+ + AVVK + +P + L + + A + L + P+ V+ G + L+ +C+++ + + + W L Y+ + Q V+D+G VP LV + ++ ++ A A+ +I + + Q V++ ++H ++ +P K+ ++ L+ I + + V++A + P I+ L D +K A I + Sbjct: 103 SDRNPPIDDLIKSGILPILVKCLERDDNPSLQFEAAWALTNIASGTSAQTQAVVKSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFINPSIPITFLRNVTWVIVNLCRNKDPPPPMETVQEVRGVCVCVKDLNCCILVDTVWALSYLTDGGNEQIQMVIDSGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDVLSHFPNLLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHQLAKGDFGTQKEAAWAISNLT 397 HSP 2 Score: 56.9954 bits (136), Expect = 3.147e-8 Identity = 55/247 (22.27%), Postives = 109/247 (44.13%), Query Frame = 2 Query: 98 YQKSRTQFVQTIAELANRPQNIETLQ-----CAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDIC-KHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQI----AKHSVDLAEIVVE 808 + ++ T + + + P +ET+Q C V +L +L+D V AL L + ++ V+ ++P LV L+ Q+ + AA + + + E Q V++C L L + + + W L I N Q Q+V+DAG +P+++ + + D G ++ AA A+S++ D + +V I ++ D+++ + + L I + + +AEI+ E Sbjct: 218 FLRNVTWVIVNLCRNKDPPPPMETVQEVRGVCVCVKDLNCCILVDTV--------WALSYLTDGGNEQIQMVIDSGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDVLSHFPNLLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHQLAKGDFGTQKEAAWAISNLTISGRKDQVEYLVQQNVIPPFCNLLSVKDSQVVQVVLDGLKNILIMAGEEASTIAEIIEE 456 HSP 3 Score: 50.0618 bits (118), Expect = 4.601e-6 Identity = 53/274 (19.34%), Postives = 111/274 (40.51%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPD 1234 ++ G L LV CLE D P+++ +AW L IA + Q+VV + AVPL + + P + A AL +I P V+ G + L + NP + R + + + ++ D + + + C+KD + + + + + E Q+++++G V +V + + + + +G + ++ + V+ + ++ + E I W L I + +AV ++P ++ + D Sbjct: 112 LIKSGILPILVKCLERDDNPSLQFEAAWALTNIASGTSAQTQAVVKSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFI-NPSIPITFLRNVTWVIVNLCRNK-DPPPPMETVQEVRGVCVCVKDLNCCILVDTVWALSYLTDGGNEQIQMVIDSGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDVLSHFPNLLTHPKE-KINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHQLAKGD 382
BLAST of Sperm associated antigen 6 vs. Ensembl Cavefish
Match: kpna6 (karyopherin subunit alpha 6 [Source:NCBI gene;Acc:103046157]) HSP 1 Score: 60.4622 bits (145), Expect = 2.561e-9 Identity = 62/267 (23.22%), Postives = 114/267 (42.70%), Query Frame = 2 Query: 179 AGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRF-YKKAASFVLRAV--AKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIRE-IAKHTPELSQLIVNAGGVAAIVDFI 967 AG + + LL +Q+ A ALG +A S V+ +ILP L+ L + R + A + L + K P + V C L L L D + + W L Y++ + Q+V+D+G LV + D + A A+ +I Q V++ A+ L ++ + ++++ ++ I + + V++A IFP ++ L+ + +K A I + TPE + +VN G + + D + Sbjct: 163 AGAVPVFIQLLNSEFEDVQEQAVWALGNIAGDSAVCRDFVLNCNILPPLLMLLTKSTRLTMTRNAVWALSNLCRGKSPPPEFEKVSPC--LPVLSRLLFSSDSDLLADACWALSYLSDGPNEKIQAVIDSGVCRRLVELLMHSDYKVASPALRAVGNIVTGDDIQTQVVLNCSALPCLLHLLSSAKESIRKEACWTISNITAGNRAQIQTVIDANIFPVLIEVLQKAEFRTRKEAAWAITNATSGGTPEQIRYLVNLGCIKPLCDLL 427
BLAST of Sperm associated antigen 6 vs. Ensembl Sea Lamprey
Match: kpna7 (karyopherin alpha 7 (importin alpha 8) [Source:ZFIN;Acc:ZDB-GENE-040426-2023]) HSP 1 Score: 63.1586 bits (152), Expect = 8.655e-11 Identity = 83/381 (21.78%), Postives = 152/381 (39.90%), Query Frame = 2 Query: 494 SAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKL-----KRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQN-------KSKKALKHILQKCVYLEALEPLLQ--------------DAPPNILK--HVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSE-YINIINNCYPEEIVKYYSPGYSEA 1549 +AW L IA ++ ++VVD GAVP V + P + A AL +I P L V+ GA+ L ++ PD + R + ++ + ++ + PA++ L +D V+ + + ++ + +++V AG V + + + + P + +G + ++ + +VI + GVL + ++ AWAL I E AV ++P L+ + + + S + HI+ V + A+EPLL DA NI + V Q K+ + GGL K++ ++ A+ + +NII+ KY+S SEA Sbjct: 115 AAWALTNIASGTSEQTKAVVDGGAVPAFVALLSSPHAHISEQAVWALGNIAGDGPVLRDLVIKYGAVEPLLALMAMPDLSMLSVSYLRNLTWTISNLCRNKKPPPPEEAVLMLLPALVRLLHHEDREVQTDATWALSYLSDGPNDRIEMVVAAGVVPRLAQLLASPELTVLTPALRTVGNIVTGTDQQTQAVI-NTGVLPVFQNLLRHQRSNVQKEAAWALSNITAGHAEQINAVITAGLIPPLVEVLHKGEFKAQREAVWAVTNLTSGGTVDHIV-YVVQVGAMEPLLSLLEVKDSSTVLIILDAITNIFQAGDKVGQTDKLC-------MMVEELGGLDKIEHLQSHENEAVYKAALNIID--------KYFSEDDSEA 478 HSP 2 Score: 60.4622 bits (145), Expect = 4.753e-10 Identity = 58/271 (21.40%), Postives = 122/271 (45.02%), Query Frame = 2 Query: 296 VVKGDILPQLVYSLAEQD-RFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIA-----ASALSDICKHS----PDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYV-AAHSETLSMSVIVSRGVL 1075 ++ ++P+ V L +D + A++ L +A + E ++VVD GA+ A V L + E + W LG IA L V+ GAV L+ + PDL + ++ +S++C++ P+ A ++ + L +++ + D +++ AL+ ++ D E+VV A + P + L + V + I T + +Q ++N G + + + + N+ L + A H+E ++ +++ G++ Sbjct: 90 IISAGLIPRFVQFLGHEDCSQIQFEAAWALTNIASGTSEQTKAVVDGGAVPAFVALLSSPHAHISEQAVWALGNIAGDGPVLRDLVIKYGAVEPLLALMAMPDLSMLSVSYLRNLTWTISNLCRNKKPPPPEEAVLML----LPALVRLLHHEDREVQTDATWALSYLSDGPNDRIEMVVAAGVVPRLAQLLASPELTVLTPALRTVGNIVTGTDQQTQAVINTGVLPVFQNLLRHQRSNVQKEAAWALSNITAGHAE--QINAVITAGLI 354 HSP 3 Score: 47.7506 bits (112), Expect = 5.368e-6 Identity = 45/182 (24.73%), Postives = 85/182 (46.70%), Query Frame = 2 Query: 311 ILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQI-AKHSVDLAEIVVEAEIFPAILTCLKDKD 853 +LP LV L +DR + A++ L ++ + + VV G + L L + V + T+G I Q Q+V++ G +P+ ++ +++ AA ALS+I + V+ AG I L +++ + K +R+ A+ + + +VD VV+ +L+ L+ KD Sbjct: 227 LLPALVRLLHHEDREVQTDATWALSYLSDGPNDRIEMVVAAGVVPRLAQLLASPELTVLTPALRTVGNIVTGTDQQTQAVINTGVLPVFQNLLRHQRSNVQKEAAWALSNITAGHAEQINAVITAGLIPPLVEVLHKGEFKAQREAVWAVTNLTSGGTVDHIVYVVQVGAMEPLLSLLEVKD 408
BLAST of Sperm associated antigen 6 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005568.1 (pep scaffold:Pmarinus_7.0:GL477092:38612:43101:1 gene:ENSPMAG00000005012.1 transcript:ENSPMAT00000005568.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 57.7658 bits (138), Expect = 3.402e-9 Identity = 58/280 (20.71%), Postives = 112/280 (40.00%), Query Frame = 2 Query: 395 KHSPELAQSVVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPD-----TKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLR 1216 KH P ++ G + V CL+ D P ++ +AW L IA + VV AGAVP LV V P + A AL +I + VV++GA+ L ++ N + L R + L+ + ++ + + P ++ L D ++ + A + + T E L+ G +V +G + P + +G + ++ V++ G L + + + +I WA+ + + + + ++P L+ Sbjct: 8 KHPP--FNDIIAAGLVPEFVACLDRDDCPLLQFEAAWALTNIASGTGEQTAIVVAAGAVPALVRLVSSPHSHISEQAVWALGNIAGDGAEHRDLVVNSGAVGPLVALLENCNIDEFHVGLLRNLTWTLSNLCRNKNPAPSVTTVLMLLPTMVRLLHHADHEIEADAAWALSYLTDGTNERIHLVQAQGVTPRLVQLLGCDVPAVVTPCLRAIGNMVTGTDE-QTQVVLKAGALDMFPMLLRHPKSNIQKEATWAVSNVAAGPIDQIEQIIAAGLVPHLIH 284 HSP 2 Score: 53.1434 bits (126), Expect = 8.504e-8 Identity = 57/250 (22.80%), Postives = 106/250 (42.40%), Query Frame = 2 Query: 296 VVKGDILPQLVYSLAEQD-RFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLC----VQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAA 1027 ++ ++P+ V L D + A++ L +A + E VV GA+ ALV + + E + W LG IA A+ VV++GAV PL+ L + E +GL R LS++C++ + + +++ + D +++ AL+ + + + +V + P ++ L V C I + T E +Q+++ AG + + K NI + VAA Sbjct: 15 IIAAGLVPEFVACLDRDDCPLLQFEAAWALTNIASGTGEQTAIVVAAGAVPALVRLVSSPHSHISEQAVWALGNIAGDGAEHRDLVVNSGAVGPLVALLENCNIDEFHVGLLRNLTWTLSNLCRNKNPAPSVTTVLMLLPTMVRLLHHADHEIEADAAWALSYLTDGTNERIHLVQAQGVTPRLVQLLGCDVPAVVTPCLRAIGNMVTGTDEQTQVVLKAGALDMFPMLLRHPKSNIQKEATWAVSNVAA 264
BLAST of Sperm associated antigen 6 vs. Ensembl Sea Lamprey
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:ZFIN;Acc:ZDB-GENE-030131-5584]) HSP 1 Score: 56.225 bits (134), Expect = 1.197e-8 Identity = 43/185 (23.24%), Postives = 84/185 (45.41%), Query Frame = 2 Query: 251 ALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDIC-KHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIV 802 AL L + ++ V+ ++P LV L+ Q+ + AA + + + E Q V++C AL L + + + W L I N Q Q+V+DAG +P+++ + + D G ++ AA A+S++ D + +V I ++ D ++ + + L I K + + E + Sbjct: 268 ALSYLTDGGNEQIQMVIDSGVVPYLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHAKEKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHHLAKGDFGTQKEAAWAISNLTISGRKDQVEYLVRENVIPPFCALLAVKDPQVVQVVLDGLNNILKMAGEEVETI 452 HSP 2 Score: 52.7582 bits (125), Expect = 1.500e-7 Identity = 34/153 (22.22%), Postives = 70/153 (45.75%), Query Frame = 2 Query: 440 LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA 898 L AL + + D + + W L Y+ + Q V+D+G VP LV + ++ ++ A A+ +I + + Q V++ A++H ++ + K+ ++ L+ I + + V++A + P I+ L D +K A I + Sbjct: 247 LPALCVLIHHTDINILVDTVWALSYLTDGGNEQIQMVIDSGVVPYLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHAKEKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHHLAKGDFGTQKEAAWAISNLT 399 HSP 3 Score: 48.9062 bits (115), Expect = 2.206e-6 Identity = 60/282 (21.28%), Postives = 122/282 (43.26%), Query Frame = 2 Query: 143 ANRPQNIETLQCAGVMNLL-RPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAV--AKHSPELAQSVVDCGALDALVICLEEFDPAVK----EGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEI---FPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIV 958 ++R I+ L +G++ +L + L + PS+Q AA AL +A+ + AVVK + +P + L + + A + L + A P+ V+ G + L L +PA+ W + + R+ +P L + + D+ + ALS + + Q V+D+G + +L ++ + + K++ A+ I + + ++V+ + FPA+LT K+K + K + I + Q +++AG + I+ Sbjct: 102 SDRNPPIDDLINSGILPILVQCLEREDNPSLQFEAAWALTNIASGTSAQTQAVVKSNAVPLFLKLLHSPHQNVCEQAVWALGNIIAAGDGPQCRDYVISLGVVKPL---LAFINPAIPITFLRNVTWVIVNLCRNKDPPPPMETIQEILPALCVLIHHTDINILVDTVWALSYLTDGGNEQIQMVIDSGVVPYLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHAKEK---INKEAVWFLSNITAGNQQQVQAVIDAGLIPMII 377
BLAST of Sperm associated antigen 6 vs. Ensembl Sea Lamprey
Match: kpna6 (karyopherin alpha 6 (importin alpha 7) [Source:ZFIN;Acc:ZDB-GENE-130530-626]) HSP 1 Score: 45.4394 bits (106), Expect = 7.291e-6 Identity = 30/121 (24.79%), Postives = 62/121 (51.24%), Query Frame = 2 Query: 416 QSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSD-ICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAK 775 Q +++C AL L+ L ++K+ + WT+ I + Q+V+DA P+L+ +Q+ + ++ AA A+++ +P+ + +V+ G I L ++ D K+ + + L I K Sbjct: 44 QVILNCSALPCLLHLLSNPKESIKKEACWTISNITAGSRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRFLVEQGCIKPLCDLLTVMDAKIVQVALNGLENILK 164
BLAST of Sperm associated antigen 6 vs. Ensembl Yeast
Match: SRP1 (Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation [Source:SGD;Acc:S000005133]) HSP 1 Score: 58.9214 bits (141), Expect = 1.004e-9 Identity = 62/304 (20.39%), Postives = 125/304 (41.12%), Query Frame = 2 Query: 314 LPQLVYSLAEQDRFYKKAASFVLRAV--AKHSPELAQSVVDCGALDALVICLEEFDPAVKE-GSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLR 1216 LPQ+ L D + +A+ R + +H P + V+ G + LV + E P + + +AW L IA + + VVDA AVPL + + + +K A AL ++ S D V+ A+ + + + L R L+ + + + V ++ P + + D + I ++ E Q +++ +V+ + + P + +G + ++ L V+++ GVL + + +++I W + I E +AV N++P L++ Sbjct: 89 LPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPI-DVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGND-LQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVK 390 HSP 2 Score: 56.6102 bits (135), Expect = 5.763e-9 Identity = 66/292 (22.60%), Postives = 119/292 (40.75%), Query Frame = 2 Query: 95 QYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPS-IQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFI 967 Q Q S T + I +RP I+ + AGV+ L + + P +Q AA AL +A+ + VV D +P + L K+ A + L VA S + V+ C A++ ++ P++ + WTL + R V + A+P L + D A A+S + + Q V+D L +++ + T ++ A+ I + ++V+ A + PA+ L + +KK I I E Q +++A + +V + Sbjct: 102 QEQLSATVKFRQILSREHRPP-IDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLL 392 HSP 3 Score: 50.447 bits (119), Expect = 4.850e-7 Identity = 52/255 (20.39%), Postives = 108/255 (42.35%), Query Frame = 2 Query: 296 VVKGDILPQLVYSLAE-QDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVI 1057 V++ ++P+LV + E Q + A++ L +A + + VVD A+ + L VKE + W LG +A + V+ A+ ++ L R A LS++C+ V + A+ LA+++ + DT+ A++ ++ + + V++ I ++ L + +V+ + I +Q+++NAG + A+ + K NI + + A + +VI Sbjct: 126 VIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVI 380 HSP 4 Score: 50.447 bits (119), Expect = 5.619e-7 Identity = 50/229 (21.83%), Postives = 97/229 (42.36%), Query Frame = 2 Query: 242 AALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICK---HSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSV-----------DLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLI 886 A A+ L++ + AV+ I +LV L+ + + A + + + Q V++ G L AL + L +K+ + WT+ I N + Q+V+DA +P LV ++ + K+ A A+S+ PD+ + +V G I L ++ D ++ AL I K + A+ + +A I C ++++D + + +I Sbjct: 277 ACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKII 505
BLAST of Sperm associated antigen 6 vs. Ensembl Nematostella
Match: EDO42228 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S260]) HSP 1 Score: 858.596 bits (2217), Expect = 0.000e+0 Identity = 413/506 (81.62%), Postives = 461/506 (91.11%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MS RQVLQVFEQYQKSRT FVQT+AELA+RPQNIETLQ AGVM+LLRPLLLD+VP+IQQTAALALGRLAN++DDLA AVVK DILPQLVYSLAEQ+RFYKKAA+FVLRAVAKHS + AQ VVDCGALDALVICLEEFDP VKE +AW LGYIARHNA+L+Q+VVDAGAVPLLVLC+QEP+L LKRIA+SALSDICKHSP+LAQTVVDAGAIAHLAQM+LN D KLKRQ+FSAL+QIAKHSVDLAE+VVEAEIFPA+L CLKD D+ V KN ATLIREIAKHTPEL+QLIVNAGGVAA+VD++G+ GN+ LPG+MMLGYVAAHSE L+MSVIVS+GV+QLS + EDHI AA AWALGQIGRHTPEH+KAVA NVLPKLL+CYLRPD SEDLQ K+KKALK+ILQKC +L ALEPLL DAPPNILKHVV QF+KVLP++ +ARRLFVTSGGLKKVQ++K EPGSAL EYIN INNCYPEEIV+YYSPGYS+ LLERVE YQP Sbjct: 1 MSTRQVLQVFEQYQKSRTSFVQTVAELASRPQNIETLQNAGVMSLLRPLLLDIVPTIQQTAALALGRLANYNDDLAEAVVKADILPQLVYSLAEQNRFYKKAAAFVLRAVAKHSAQHAQCVVDCGALDALVICLEEFDPGVKEAAAWALGYIARHNAELSQAVVDAGAVPLLVLCIQEPELALKRIASSALSDICKHSPELAQTVVDAGAIAHLAQMILNTDAKLKRQVFSALSQIAKHSVDLAEMVVEAEIFPAVLNCLKDPDEYVCKNVATLIREIAKHTPELAQLIVNAGGVAAVVDYVGDKSGNVRLPGVMMLGYVAAHSENLAMSVIVSKGVVQLSISIAEESEDHIQAAAAWALGQIGRHTPEHAKAVAVANVLPKLLQCYLRPDSSEDLQTKAKKALKNILQKCTHLPALEPLLHDAPPNILKHVVGQFAKVLPHDAKARRLFVTSGGLKKVQEIKAEPGSALHEYINTINNCYPEEIVRYYSPGYSDRLLERVEQYQP 506
BLAST of Sperm associated antigen 6 vs. Ensembl Nematostella
Match: EDO30648 (Importin subunit alpha [Source:UniProtKB/TrEMBL;Acc:A7T055]) HSP 1 Score: 83.1889 bits (204), Expect = 6.679e-17 Identity = 67/311 (21.54%), Postives = 141/311 (45.34%), Query Frame = 2 Query: 314 LPQLVYSL-AEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPD 1234 LP +V +L + D + V + ++K V++ G + LV L+ FD P ++ +AW L IA ++ ++V + GAVP+ V + + A AL +I P + TV+ GA+ L ++ P T ++ R + L+ + ++ ++ ++ PAI + D V + + + E QL++++G V +V+ +G + N+ P + +G + + + +VI + + ++ + + +I+ AWA+ + T + +AV ++P ++ R D Sbjct: 72 LPVVVGNLQSGDDNLMFTSVQSVRKMLSKEKSPPIDDVINAGLIPMLVKLLDRFDLPLLQFEAAWALTNIASGTSEQTKAVQEGGAVPMFVKLLSSVHDNVAEQAVWALGNIAGDGPLMRNTVIACGALGPLLALI-KPSTSIQFLRNVTWTLSNLCRNKNPPPDVTAVQQVLPAIARLILSDDKEVLADTCWALSYLTDGVNERIQLVLDSGVVPKLVELLGYPEVNVLTPALRAIGNIVTGRDDQTQAVIDTGALFYFRALLTHK-KSNIIKEAAWAISNVTAGTAQQIQAVMDSGLVPLIVDILRRGD 380 HSP 2 Score: 62.003 bits (149), Expect = 3.567e-10 Identity = 66/302 (21.85%), Postives = 132/302 (43.71%), Query Frame = 2 Query: 122 VQTIAELANRPQN--IETLQCAGVMNLLRPLL--LDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDAL--------------------------------VICLEEFDPAV--------KE---GSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLI 886 VQ++ ++ ++ ++ I+ + AG++ +L LL D+ P +Q AA AL +A+ + + AV +G +P V L+ + A + L +A P + +V+ CGAL L V +++ PA+ KE + W L Y+ + Q V+D+G VP LV + P++ + A A+ +I D Q V+D GA+ + ++ + + + ++ A++ + + + V+++ + P I+ L+ D +K +I Sbjct: 91 VQSVRKMLSKEKSPPIDDVINAGLIPMLVKLLDRFDL-PLLQFEAAWALTNIASGTSEQTKAVQEGGAVPMFVKLLSSVHDNVAEQAVWALGNIAGDGPLMRNTVIACGALGPLLALIKPSTSIQFLRNVTWTLSNLCRNKNPPPDVTAVQQVLPAIARLILSDDKEVLADTCWALSYLTDGVNERIQLVLDSGVVPKLVELLGYPEVNVLTPALRAIGNIVTGRDDQTQAVIDTGALFYFRALLTHKKSNIIKEAAWAISNVTAGTAQQIQAVMDSGLVPLIVDILRRGDFKARKEAIWVI 391
BLAST of Sperm associated antigen 6 vs. Ensembl Nematostella
Match: EDO29161 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4F4]) HSP 1 Score: 55.8398 bits (133), Expect = 2.048e-8 Identity = 42/173 (24.28%), Postives = 82/173 (47.40%), Query Frame = 2 Query: 296 VVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDIC-KHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEA 811 V++ ++ LV L+ Q+ + AA + + + E Q V++ GAL L +K+ + W L I N Q V+DAG +PL++ ++ + ++ AA A+S++ + +V A+ HL +++ DT++ + + L I K + D A ++ A Sbjct: 169 VIESGVVQYLVPLLSHQEIKLQTAALRAVGNIVTGTDEQTQVVLNHGALSHFTGLLTHHKEKIKKEAVWFLSNITAGNQSQVQEVIDAGLIPLIIQTLETGEFQTQKEAAWAISNMTVSGRREQVAVLVHCNAVEHLCKLLDVRDTQVVQIVLDGLNNILKMAGDKATVIATA 341 HSP 2 Score: 53.5286 bits (127), Expect = 1.022e-7 Identity = 34/153 (22.22%), Postives = 72/153 (47.06%), Query Frame = 2 Query: 440 LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA 898 L AL + + D + + W L Y+ + Q V+++G V LV + ++ L+ A A+ +I + + Q V++ GA++H ++ + K+K++ L+ I + + V++A + P I+ L+ + +K A I + Sbjct: 133 LPALALLIHHQDLDILVDTVWALSYLTDGGSHQIQLVIESGVVQYLVPLLSHQEIKLQTAALRAVGNIVTGTDEQTQVVLNHGALSHFTGLLTHHKEKIKKEAVWFLSNITAGNQSQVQEVIDAGLIPLIIQTLETGEFQTQKEAAWAISNMT 285 HSP 3 Score: 50.0618 bits (118), Expect = 1.624e-6 Identity = 56/266 (21.05%), Postives = 112/266 (42.11%), Query Frame = 2 Query: 434 GALDALVICLEEFDP-AVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAE---IVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRC 1219 G L LV CL+ D +++ +AW L IA ++ Q+VV+AGAVP + + P + A AL +I P L V+ G + L + V NP + Q +A A I+ +V +++ ++ PA+ + +D + + + + QL++ +G V +V + + + + +G + ++ V+++ G L + ++ I W L I + V ++P +++ Sbjct: 3 GILPILVQCLQREDNHSLQFEAAWALTNIASGTSEQTQAVVNAGAVPFFLKLLNSPRQHVCEQAVWALGNIVGDGPVLRDFVIGYGVVKPLLKFV-NPGVPFQTQKEAAWA-ISNMTVSGRREQPLLLLFQVLPALALLIHHQDLDILVDTVWALSYLTDGGSHQIQLVIESGVVQYLVPLLSHQEIKLQTAALRAVGNIVTGTDE-QTQVVLNHGALSHFTGLLTHHKEKIKKEAVWFLSNITAGNQSQVQEVIDAGLIPLIIQT 265
BLAST of Sperm associated antigen 6 vs. Ensembl Nematostella
Match: EDO31940 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWI7]) HSP 1 Score: 54.6842 bits (130), Expect = 5.687e-8 Identity = 55/269 (20.45%), Postives = 110/269 (40.89%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEFDP-AVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRC 1219 ++ G L LV CL+ D +++ +AW L IA ++ Q+VV+AGAVP + + P + A AL +I P L V+ G + L + V NP + R + L + ++ I E+ PA+ + +D + + + + QL++ +G V +V + + + + +G + ++ V+++ G L + ++ I W L I + V ++P +++ Sbjct: 103 LIKSGILPILVQCLQREDNHSLQFEAAWALTNIASGTSEQTQAVVNAGAVPFFLKLLNSPRQHVCEQAVWALGNIVGDGPVLRDFVIGYGVVKPLLKFV-NPGVPITFLRNVTWVLVNLCRNKEPPPPIDTIREVLPALALLIHHQDLDILVDTVWALSYLTDGGSHQIQLVIESGVVQYLVPLLSHQEIKLQTAALRAVGNIVTGTDE-QTQVVLNHGALSHFTGLLTHHKEKIKKEAVWFLSNITAGNQSQVQEVIDAGLIPLIIQT 369 HSP 2 Score: 51.2174 bits (121), Expect = 7.362e-7 Identity = 31/135 (22.96%), Postives = 66/135 (48.89%), Query Frame = 2 Query: 440 LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLK 844 L AL + + D + + W L Y+ + Q V+++G V LV + ++ L+ A A+ +I + + Q V++ GA++H ++ + K+K++ L+ I + + V++A + P I+ L+ Sbjct: 237 LPALALLIHHQDLDILVDTVWALSYLTDGGSHQIQLVIESGVVQYLVPLLSHQEIKLQTAALRAVGNIVTGTDEQTQVVLNHGALSHFTGLLTHHKEKIKKEAVWFLSNITAGNQSQVQEVIDAGLIPLIIQTLE 371
BLAST of Sperm associated antigen 6 vs. Ensembl Nematostella
Match: EDO38337 (Importin subunit alpha [Source:UniProtKB/TrEMBL;Acc:A7SD78]) HSP 1 Score: 53.1434 bits (126), Expect = 2.124e-7 Identity = 58/310 (18.71%), Postives = 126/310 (40.65%), Query Frame = 2 Query: 296 VVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELA-QSVVDCGALDALVICLE-EFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 + +G + +V ++ D + AA+ R + P V+ CG + V L+ E + A++ +AW L IA + + V+AGAVP + ++ P ++ A AL +I S D V++ G + + L T+L R + AL+ + + + + P + L D V + + + E Q ++++G +V+ + + ++ + +G + + + ++ + L + ++ ++ I W + I +AV N++P L+ Sbjct: 69 MAEGPLFQDMVQAIISDDVEMQLAATQRFRKILSKEPNPPIDDVIKCGVIPKFVEFLQREDNSALQFEAAWALTNIASGTSMQTRETVNAGAVPCFIKLLESPKEEVQEQAVWALGNIAGDSADCRDYVLNCGVLTPFLNL-LTKSTRLSMTRNLVWALSNLCRGRHPPPDFAKVSPCLPLLSRLLFSTDAEVLADTCWALSYLLDGPNEKIQAVIDSGVCRRLVELLDHNQNSVVSAALRAVGNIVTGDDIQTQVILNCHALPNLLHLLSS-TKESIRKEACWTISNITAGNRLQIQAVIDANIIPMLI 376
BLAST of Sperm associated antigen 6 vs. Ensembl Medaka
Match: spag6 (sperm associated antigen 6 [Source:NCBI gene;Acc:101168253]) HSP 1 Score: 729.169 bits (1881), Expect = 0.000e+0 Identity = 346/506 (68.38%), Postives = 427/506 (84.39%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQP 1579 MSQRQ++QVFEQYQKSR QFVQTI++LA RPQNIE L AGVM+LLRPLLLDVVP +Q TAALALGRLA+ S+ LA AVVK +ILP +V S+ +RF KKAA+FVLRAV+KHSPEL+Q+VVDCG +DALV CL EFDP VKE +AW+LG ARHNA L+Q+VV+AGAVPLLVLC+ EP++ LKRIAAS LSDICKH+P+LA VVDAGAI HLAQ++L+ DTKLKRQ+FSAL+QIAKHS DL+E+V E +FPA +TCLKD D+ V+KN TL+RE+AK T ELSQL+VN GG+ A++D++ + G++ LPGIMMLGYVA+HSE+L+M+VI+S+GV QL+ + + EDHI AAT W++GQIG+HTP+H+KAVA N+LPK+L+ Y SEDLQ KSKKALK ILQKC +L ALEPLL DAP ILKHV+ QFSKVLP++ +ARRLFVTSGGLKKVQ+++ EPGS L E+IN IN+C+PEEIV+Y+SPGYSE LLER+E+YQP Sbjct: 1 MSQRQIIQVFEQYQKSRMQFVQTISDLAARPQNIEILHNAGVMSLLRPLLLDVVPGVQHTAALALGRLADSSNSLAEAVVKENILPDVVQSVTSHNRFQKKAAAFVLRAVSKHSPELSQAVVDCGGVDALVQCLMEFDPGVKEAAAWSLGCTARHNATLSQTVVNAGAVPLLVLCLLEPEMALKRIAASTLSDICKHTPELAHAVVDAGAIPHLAQLILSRDTKLKRQVFSALSQIAKHSADLSEMVAEVGVFPAAMTCLKDPDEYVRKNVTTLMREMAKQTAELSQLVVNCGGLGAVIDYLNDCSGSLRLPGIMMLGYVASHSESLAMAVILSKGVSQLAVCLSEEPEDHIKAATVWSIGQIGQHTPDHAKAVATANLLPKILKLYTDASSSEDLQAKSKKALKSILQKCTHLPALEPLLYDAPSKILKHVMCQFSKVLPHDSKARRLFVTSGGLKKVQEIQAEPGSVLQEHINAINSCFPEEIVRYHSPGYSEVLLERLENYQP 506
BLAST of Sperm associated antigen 6 vs. Ensembl Medaka
Match: kpna2 (importin subunit alpha-1 [Source:NCBI gene;Acc:101160561]) HSP 1 Score: 65.0846 bits (157), Expect = 9.096e-11 Identity = 67/324 (20.68%), Postives = 132/324 (40.74%), Query Frame = 2 Query: 395 KHSPELAQSVVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTK-----LKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHI-----LQKCVYL---EALEPLL 1324 KH P ++ G + V L D P ++ ++W L IA + +VV+ GA+P + V P L + A AL +I L V+ GA+A L ++ PD R + L+ + ++ I ++ PA++ L D V + + + + + +++ G + +V +G + + P + +G + ++ + +V+ + + + + + +I AW L I + V ++P L+ +R D Q ++ A+ + +Q+ VYL LEPLL Sbjct: 63 KHPP--IDQMISAGLIPKFVAFLGLMDFPPIQFEASWALTNIASGTSDQTAAVVEGGAIPAFINLVTSPHLHISEQAVWALGNIAGDGSALRDRVIKHGAVAPLLSLLAVPDLNVFTPGFLRNVTWTLSNLCRNKNPSPPISAVQQVMPALIRLLHHDDREVLADACWAVSYLTDGSNDRIEIVAGTGLIPRLVKLLGFEELPVITPALRAIGNIVTGTDEQTQAVLDAGALFMFPKLLRHK-KPNIQKEAAWTLSNITAGKDSQIQEVIDAGLVPYLVDLLVRADYKT--QKEAVWAVTNFTSGGTVQQVVYLVQANVLEPLL 381 HSP 2 Score: 53.5286 bits (127), Expect = 3.355e-7 Identity = 64/266 (24.06%), Postives = 115/266 (43.23%), Query Frame = 2 Query: 296 VVKGDILPQLVYSLAEQDRF--YKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDL-----GLKRIAASALSDICKH---SPDLA--QTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVI 1057 ++ ++P+ V L D F + AS+ L +A + + +VV+ GA+ A + + + E + W LG IA + L V+ GAV L+ + PDL G R LS++C++ SP ++ Q V+ A L +++ + D ++ A++ + S D EIV + P ++ L ++ V I I T E +Q +++AG + + K NI L + A ++ VI Sbjct: 70 MISAGLIPKFVAFLGLMD-FPPIQFEASWALTNIASGTSDQTAAVVEGGAIPAFINLVTSPHLHISEQAVWALGNIAGDGSALRDRVIKHGAVAPLLSLLAVPDLNVFTPGFLRNVTWTLSNLCRNKNPSPPISAVQQVMPA-----LIRLLHHDDREVLADACWAVSYLTDGSNDRIEIVAGTGLIPRLVKLLGFEELPVITPALRAIGNIVTGTDEQTQAVLDAGALFMFPKLLRHKKPNIQKEAAWTLSNITAGKDSQIQEVI 329
BLAST of Sperm associated antigen 6 vs. Ensembl Medaka
Match: kpna2 (importin subunit alpha-1 [Source:NCBI gene;Acc:101160561]) HSP 1 Score: 65.0846 bits (157), Expect = 1.045e-10 Identity = 67/324 (20.68%), Postives = 132/324 (40.74%), Query Frame = 2 Query: 395 KHSPELAQSVVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTK-----LKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHI-----LQKCVYL---EALEPLL 1324 KH P ++ G + V L D P ++ ++W L IA + +VV+ GA+P + V P L + A AL +I L V+ GA+A L ++ PD R + L+ + ++ I ++ PA++ L D V + + + + + +++ G + +V +G + + P + +G + ++ + +V+ + + + + + +I AW L I + V ++P L+ +R D Q ++ A+ + +Q+ VYL LEPLL Sbjct: 102 KHPP--IDQMISAGLIPKFVAFLGLMDFPPIQFEASWALTNIASGTSDQTAAVVEGGAIPAFINLVTSPHLHISEQAVWALGNIAGDGSALRDRVIKHGAVAPLLSLLAVPDLNVFTPGFLRNVTWTLSNLCRNKNPSPPISAVQQVMPALIRLLHHDDREVLADACWAVSYLTDGSNDRIEIVAGTGLIPRLVKLLGFEELPVITPALRAIGNIVTGTDEQTQAVLDAGALFMFPKLLRHK-KPNIQKEAAWTLSNITAGKDSQIQEVIDAGLVPYLVDLLVRADYKT--QKEAVWAVTNFTSGGTVQQVVYLVQANVLEPLL 420 HSP 2 Score: 53.9138 bits (128), Expect = 3.024e-7 Identity = 59/240 (24.58%), Postives = 104/240 (43.33%), Query Frame = 2 Query: 368 ASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDL-----GLKRIAASALSDICKH---SPDLA--QTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVI 1057 AS+ L +A + + +VV+ GA+ A + + + E + W LG IA + L V+ GAV L+ + PDL G R LS++C++ SP ++ Q V+ A L +++ + D ++ A++ + S D EIV + P ++ L ++ V I I T E +Q +++AG + + K NI L + A ++ VI Sbjct: 134 ASWALTNIASGTSDQTAAVVEGGAIPAFINLVTSPHLHISEQAVWALGNIAGDGSALRDRVIKHGAVAPLLSLLAVPDLNVFTPGFLRNVTWTLSNLCRNKNPSPPISAVQQVMPA-----LIRLLHHDDREVLADACWAVSYLTDGSNDRIEIVAGTGLIPRLVKLLGFEELPVITPALRAIGNIVTGTDEQTQAVLDAGALFMFPKLLRHKKPNIQKEAAWTLSNITAGKDSQIQEVI 368
BLAST of Sperm associated antigen 6 vs. Ensembl Medaka
Match: kpna4 (karyopherin subunit alpha 4 [Source:NCBI gene;Acc:101173644]) HSP 1 Score: 60.077 bits (144), Expect = 3.386e-9 Identity = 70/329 (21.28%), Postives = 130/329 (39.51%), Query Frame = 2 Query: 65 SQRQVLQVFEQYQKSRTQFVQTIAELA-------NRPQNIETLQCAGVMNLLRPLL-LDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGA-------------------------------------------LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA 898 SQ L+ Q S Q VQ A A +R I+ L +G++ +L L D PS+Q AA AL +A+ + + AVV+ + +P + L + + A + L + P+ V+ G L AL + + D ++ + W L Y+ + Q V+D+G VP LV + ++ ++ A A+ +I + + Q V++ A++H ++ +P K+ ++ L+ I + + V++A++ P I+ L D +K A I + Sbjct: 68 SQNTSLEAIVQNATSDNQGVQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVHCLDRDDNPSLQFEAAWALTNIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISPSIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVSILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDAKLVPMIIHLLDKGDFGTQKEAAWAISNLT 396 HSP 2 Score: 55.0694 bits (131), Expect = 1.356e-7 Identity = 53/274 (19.34%), Postives = 111/274 (40.51%), Query Frame = 2 Query: 422 VVDCGALDALVICLEEFD-PAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPD 1234 ++ G L LV CL+ D P+++ +AW L IA ++ Q+VV + AVPL + + P + A AL +I P V+ G + L + +P + R + + + +H + EI PA+ + D + + + + E Q+++++G V +V + + + + +G + ++ + V+ + ++ + E I W L I + +AV ++P ++ + D Sbjct: 110 LIKSGILPILVHCLDRDDNPSLQFEAAWALTNIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFI-SPSIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVSILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKE-KINKEAVWFLSNITAGNQQQVQAVIDAKLVPMIIHLLDKGD 381
BLAST of Sperm associated antigen 6 vs. Ensembl Medaka
Match: KPNA2 (karyopherin subunit alpha 2 [Source:NCBI gene;Acc:101159727]) HSP 1 Score: 60.077 bits (144), Expect = 3.563e-9 Identity = 88/422 (20.85%), Postives = 165/422 (39.10%), Query Frame = 2 Query: 332 SLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALV--ICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPD-----TKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQ------NKSKKALKHILQKCVYLEALEPLLQ--------------DAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSAL-SEYINIINNCYPEE 1513 +L Q + + A +F+ R + SP + ++ G + LV + L E +P ++ +AW L IA + +SV+ GAVP V + P + + A AL +I D V+ G + L ++ D + R + AL+ + +H + ++ P + L D V + + + E +++V+ V +V + +I P + +G + ++ + V+ + G L + + +I AWA+ I + V ++P L+ + D + N + + V+ ALEPL+Q DA NIL Q SK + R L GGL ++ ++ A+ +NII+ + +E Sbjct: 83 NLEHQVQAVQAARAFLSR---EKSPPV-DGIIAAGLIPTLVGFLALSE-NPPIQFEAAWALTNIASGTSHQTKSVIAGGAVPAFVSLISSPHQHISQQAIWALGNIAGDGADSRDLVIHHGGLQPLLALLETSDLSVFGSSYLRNVTWALSNLCRHKNPAPPLASVRQMLPVLAHLLHHDDREVLADTCRALSHLTDGANERIEMVVDTDCVPRLVQLLSCEDVSIVTPALRTIGNIVTGTDEQTQCVL-NAGALAVFPVLLGYPNPNIQKEAAWAVSNITAGKSSQIQEVINAGLVPMLVDILQQGDYKTQREAVWAVTNYTSGGTVDQVAYLVHCNALEPLIQLLHIKDSRVILVILDAIDNIL-----QMSKDTGEVEKVRLLIEGLGGLDTLEALQSHDNEAVYKSSLNIIDKYFSQE 493 HSP 2 Score: 58.5362 bits (140), Expect = 1.068e-8 Identity = 48/184 (26.09%), Postives = 88/184 (47.83%), Query Frame = 2 Query: 251 ALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSP-DLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEI 799 AL L + +++ VV D +P+LV L+ +D A + + + E Q V++ GAL + L +P +++ +AW + I + Q V++AG VP+LV +Q+ D +R A A+++ D +V A+ L Q++ D+++ I A+ I + S D E+ Sbjct: 268 ALSHLTDGANERIEMVVDTDCVPRLVQLLSCEDVSIVTPALRTIGNIVTGTDEQTQCVLNAGALAVFPVLLGYPNPNIQKEAAWAVSNITAGKSSQIQEVINAGLVPMLVDILQQGDYKTQREAVWAVTNYTSGGTVDQVAYLVHCNALEPLIQLLHIKDSRVILVILDAIDNILQMSKDTGEV 451
BLAST of Sperm associated antigen 6 vs. Planmine SMEST
Match: SMESG000018022.1 (SMESG000018022.1) HSP 1 Score: 1035.4 bits (2676), Expect = 0.000e+0 Identity = 508/508 (100.00%), Postives = 508/508 (100.00%), Query Frame = 2 Query: 62 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPAL 1585 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPAL Sbjct: 1 MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLLDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLLRCYLRPDVSEDLQNKSKKALKHILQKCVYLEALEPLLQDAPPNILKHVVAQFSKVLPNNPQARRLFVTSGGLKKVQDMKVEPGSALSEYINIINNCYPEEIVKYYSPGYSEALLERVESYQPAL 508
BLAST of Sperm associated antigen 6 vs. Planmine SMEST
Match: SMESG000055942.1 (SMESG000055942.1) HSP 1 Score: 56.9954 bits (136), Expect = 2.020e-8 Identity = 78/374 (20.86%), Postives = 151/374 (40.37%), Query Frame = 2 Query: 110 RTQFVQTIAELANRPQN--IETLQCAGVMNLLRPLL-LDVVPSIQQTAALALGRLANHSDDLALAVVKGDILP---------------QLVYSLAE--------QD------------RFYK--------KAASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA-KHTPELSQLIVNAGGVAAIVDFIGETKG---NISLPGIMMLGYVAAHSETLSMSVIVSRGVLQL 1081 R + +Q +L + +N I+ L +G+M +L L D P+IQ AA AL +A+ + + AVV+ +P Q V++L +D +F+K + ++V+ + ++ V L AL ++ D + + W L Y+ Q V++A V LV + D+ ++ A A+ +I + + Q V++ A++H Q++ + K+ ++ L+ I + + + V++ + P I+ L D +K A +I + TP+ +V G + + + L GI+ + VA L ++I G L L Sbjct: 78 RLEAIQAARKLLSSDKNPPIDNLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFFKCRCTNNLLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQEQVQAVIDYSLIPLIIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCKMLSVRDAQVVQVVLDGILNILKVANDQSDLVCTMIEECGGLDL 451 HSP 2 Score: 50.447 bits (119), Expect = 2.533e-6 Identity = 62/312 (19.87%), Postives = 119/312 (38.14%), Query Frame = 2 Query: 419 SVVDCGALDALVICLE-EFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQ-------------------------------------------MVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCED-HILAATAWALGQIG-RHTPEHSKAVAKQNVLPKLLR 1216 +++ G + LV CL+ + P ++ +AW L IA ++ +VV AGAVPL + + + A AL +I P L + G +A L + ++ N DT + AL+ + D ++V+EA+I ++ L D V+ + I T E +Q ++N ++ + +K I+ + L + A ++ + ++ ++ L I++ D D AW + + TP+ V +Q V+P + Sbjct: 99 NLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFFKCRCTNNLLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQE-QVQAVIDYSLIPL-IIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCK 408
BLAST of Sperm associated antigen 6 vs. Planmine SMEST
Match: SMESG000058125.1 (SMESG000058125.1) HSP 1 Score: 56.225 bits (134), Expect = 4.013e-8 Identity = 76/374 (20.32%), Postives = 142/374 (37.97%), Query Frame = 2 Query: 110 RTQFVQTIAELANRPQN--IETLQCAGVMNLLRPLL-LDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGA-------------------------------------------LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA-KHTPELSQLIVNAGGVAAIVDFIGETKG---NISLPGIMMLGYVAAHSETLSMSVIVSRGVLQL 1081 R + +Q +L + +N I+ L +G+M +L L D P+IQ AA AL +A+ + + AVV+ +P + L + A + L + P L + G L AL ++ D + + W L Y+ Q V++A V LV + D+ ++ A A+ +I + + Q V++ A++H Q++ + K+ ++ L+ I + + + V++ + P I+ L D +K A +I + TP+ +V G + + + L GI+ + VA L ++I G L L Sbjct: 72 RLEAIQAARKLLSSDKNPPIDNLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFLNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQEQVQAVIDYSLIPLIIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCKMLSVRDAQVVQVVLDGILNILKVANDQSDLVCTMIEECGGLDL 445 HSP 2 Score: 53.5286 bits (127), Expect = 2.866e-7 Identity = 59/286 (20.63%), Postives = 115/286 (40.21%), Query Frame = 2 Query: 368 ASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCED-HILAATAWALGQIG-RHTPEHSKAVAKQNVLPKLLR 1216 A++ L +A + E +VV GA+ + L V E + W LG I L + G V PLL + + R + ++C++ V + + L ++ N DT + AL+ + D ++V+EA+I ++ L D V+ + I T E +Q ++N ++ + +K I+ + L + A ++ + ++ ++ L I++ D D AW + + TP+ V +Q V+P + Sbjct: 119 AAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFLNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQE-QVQAVIDYSLIPL-IIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCK 402 HSP 3 Score: 50.447 bits (119), Expect = 2.648e-6 Identity = 52/268 (19.40%), Postives = 110/268 (41.04%), Query Frame = 2 Query: 419 SVVDCGALDALVICLE-EFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 +++ G + LV CL+ + P ++ +AW L IA ++ +VV AGAVPL + + + A AL +I P L + G +A L + LN D + R + + + ++ V +E+ PA+ +++ D + + + + + Q+++ A V +V + + + +G + ++ + V+ + + + E I W L I E +AV +++P ++ Sbjct: 93 NLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKF-LNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKE-KINKEAVWFLSNITAGNQEQVQAVIDYSLIPLII 358
BLAST of Sperm associated antigen 6 vs. Planmine SMEST
Match: SMESG000058125.1 (SMESG000058125.1) HSP 1 Score: 56.225 bits (134), Expect = 4.124e-8 Identity = 76/374 (20.32%), Postives = 142/374 (37.97%), Query Frame = 2 Query: 110 RTQFVQTIAELANRPQN--IETLQCAGVMNLLRPLL-LDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGA-------------------------------------------LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA-KHTPELSQLIVNAGGVAAIVDFIGETKG---NISLPGIMMLGYVAAHSETLSMSVIVSRGVLQL 1081 R + +Q +L + +N I+ L +G+M +L L D P+IQ AA AL +A+ + + AVV+ +P + L + A + L + P L + G L AL ++ D + + W L Y+ Q V++A V LV + D+ ++ A A+ +I + + Q V++ A++H Q++ + K+ ++ L+ I + + + V++ + P I+ L D +K A +I + TP+ +V G + + + L GI+ + VA L ++I G L L Sbjct: 61 RLEAIQAARKLLSSDKNPPIDNLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFLNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQEQVQAVIDYSLIPLIIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCKMLSVRDAQVVQVVLDGILNILKVANDQSDLVCTMIEECGGLDL 434 HSP 2 Score: 53.5286 bits (127), Expect = 2.544e-7 Identity = 59/286 (20.63%), Postives = 115/286 (40.21%), Query Frame = 2 Query: 368 ASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCED-HILAATAWALGQIG-RHTPEHSKAVAKQNVLPKLLR 1216 A++ L +A + E +VV GA+ + L V E + W LG I L + G V PLL + + R + ++C++ V + + L ++ N DT + AL+ + D ++V+EA+I ++ L D V+ + I T E +Q ++N ++ + +K I+ + L + A ++ + ++ ++ L I++ D D AW + + TP+ V +Q V+P + Sbjct: 108 AAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFLNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQE-QVQAVIDYSLIPL-IIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCK 391 HSP 3 Score: 50.447 bits (119), Expect = 2.502e-6 Identity = 52/268 (19.40%), Postives = 110/268 (41.04%), Query Frame = 2 Query: 419 SVVDCGALDALVICLE-EFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 +++ G + LV CL+ + P ++ +AW L IA ++ +VV AGAVPL + + + A AL +I P L + G +A L + LN D + R + + + ++ V +E+ PA+ +++ D + + + + + Q+++ A V +V + + + +G + ++ + V+ + + + E I W L I E +AV +++P ++ Sbjct: 82 NLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKF-LNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKE-KINKEAVWFLSNITAGNQEQVQAVIDYSLIPLII 347
BLAST of Sperm associated antigen 6 vs. Planmine SMEST
Match: SMESG000058125.1 (SMESG000058125.1) HSP 1 Score: 56.225 bits (134), Expect = 4.493e-8 Identity = 76/374 (20.32%), Postives = 142/374 (37.97%), Query Frame = 2 Query: 110 RTQFVQTIAELANRPQN--IETLQCAGVMNLLRPLL-LDVVPSIQQTAALALGRLANHSDDLALAVVKGDILPQLVYSLAEQDRFYKKAASFVLRAVAKHSPELAQSVVDCGA-------------------------------------------LDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIA-KHTPELSQLIVNAGGVAAIVDFIGETKG---NISLPGIMMLGYVAAHSETLSMSVIVSRGVLQL 1081 R + +Q +L + +N I+ L +G+M +L L D P+IQ AA AL +A+ + + AVV+ +P + L + A + L + P L + G L AL ++ D + + W L Y+ Q V++A V LV + D+ ++ A A+ +I + + Q V++ A++H Q++ + K+ ++ L+ I + + + V++ + P I+ L D +K A +I + TP+ +V G + + + L GI+ + VA L ++I G L L Sbjct: 78 RLEAIQAARKLLSSDKNPPIDNLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFLNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQEQVQAVIDYSLIPLIIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCKMLSVRDAQVVQVVLDGILNILKVANDQSDLVCTMIEECGGLDL 451 HSP 2 Score: 53.1434 bits (126), Expect = 3.436e-7 Identity = 59/286 (20.63%), Postives = 115/286 (40.21%), Query Frame = 2 Query: 368 ASFVLRAVAKHSPELAQSVVDCGALDALVICLEEFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAV-PLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLKRQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCED-HILAATAWALGQIG-RHTPEHSKAVAKQNVLPKLLR 1216 A++ L +A + E +VV GA+ + L V E + W LG I L + G V PLL + + R + ++C++ V + + L ++ N DT + AL+ + D ++V+EA+I ++ L D V+ + I T E +Q ++N ++ + +K I+ + L + A ++ + ++ ++ L I++ D D AW + + TP+ V +Q V+P + Sbjct: 125 AAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKFLNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKEKINKEAVWFLSNITAGNQE-QVQAVIDYSLIPL-IIYHLDKGDFQTQKEAAWVISNLTISGTPQQVAFVVQQGVIPPFCK 408 HSP 3 Score: 50.447 bits (119), Expect = 3.064e-6 Identity = 52/268 (19.40%), Postives = 110/268 (41.04%), Query Frame = 2 Query: 419 SVVDCGALDALVICLE-EFDPAVKEGSAWTLGYIARHNAQLAQSVVDAGAVPLLVLCVQEPDLGLKRIAASALSDICKHSPDLAQTVVDAGAIAHLAQMVLNPDTKLK--RQIFSALAQIAKHSVDLAEIVVEAEIFPAILTCLKDKDDMVKKNCATLIREIAKHTPELSQLIVNAGGVAAIVDFIGETKGNISLPGIMMLGYVAAHSETLSMSVIVSRGVLQLSSIFNNDCEDHILAATAWALGQIGRHTPEHSKAVAKQNVLPKLL 1213 +++ G + LV CL+ + P ++ +AW L IA ++ +VV AGAVPL + + + A AL +I P L + G +A L + LN D + R + + + ++ V +E+ PA+ +++ D + + + + + Q+++ A V +V + + + +G + ++ + V+ + + + E I W L I E +AV +++P ++ Sbjct: 99 NLIQSGIMPILVECLDNDAHPNIQFEAAWALTNIASGTSEQTNAVVQAGAVPLFLKLLGSTHSNVCEQAVWALGNIIGDGPTLRDYAISLGVVAPLLKF-LNADVPITFLRNVTWVIVNLCRNKDPPPSPVTISELLPALRFLIQNIDTNILVDTVWALSYLTDGGNDQIQMVIEADIVRFLVPLLSHNDVKVQTAALRAVGNIVTGTDEQTQYVLNFDALSHFPQLLQHSKE-KINKEAVWFLSNITAGNQEQVQAVIDYSLIPLII 364 The following BLAST results are available for this feature:
BLAST of Sperm associated antigen 6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Sperm associated antigen 6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Sperm associated antigen 6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of Sperm associated antigen 6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Sperm associated antigen 6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Sperm associated antigen 6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019318 ID=SMED30019318|Name=Sperm associated antigen 6|organism=Schmidtea mediterranea sexual|type=transcript|length=1664bpback to top protein sequence of SMED30019318-orf-1 >SMED30019318-orf-1 ID=SMED30019318-orf-1|Name=SMED30019318-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=509bp MSQRQVLQVFEQYQKSRTQFVQTIAELANRPQNIETLQCAGVMNLLRPLLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|