TLC domain-containing protein

Overview
NameTLC domain-containing protein
Smed IDSMED30019291
Length (bp)1012
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TLC domain-containing protein (SMED30019291) t-SNE clustered cells

Violin plots show distribution of expression levels for TLC domain-containing protein (SMED30019291) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TLC domain-containing protein (SMED30019291) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TLC domain-containing protein (SMED30019291) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019291

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30019291h1SMcG0019268 Contig2332GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30019291h1SMcG0019268 dd_Smed_v4_7852_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019291h1SMcG0019268 dd_Smed_v4_7852_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30019291h1SMcG0019268 dd_Smed_v6_7852_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Human
Match: CLN8 (CLN8 transmembrane ER and ERGIC protein [Source:HGNC Symbol;Acc:HGNC:2079])

HSP 1 Score: 167.548 bits (423), Expect = 4.104e-49
Identity = 90/257 (35.02%), Postives = 148/257 (57.59%), Query Frame = 2 
Query:  167 VIFASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIA------NGDVKRK 913
            ++ A  +  +GVF+    L S L  TYR L A+EK+FW +   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++  +V +   H+   + L+LEM+TPF+ + W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A      NG + RK
Sbjct:   27 LMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQLLRK 283
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Human
Match: CLN8 (CLN8 transmembrane ER and ERGIC protein [Source:HGNC Symbol;Acc:HGNC:2079])

HSP 1 Score: 167.548 bits (423), Expect = 4.104e-49
Identity = 90/257 (35.02%), Postives = 148/257 (57.59%), Query Frame = 2 
Query:  167 VIFASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIA------NGDVKRK 913
            ++ A  +  +GVF+    L S L  TYR L A+EK+FW +   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++  +V +   H+   + L+LEM+TPF+ + W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A      NG + RK
Sbjct:   27 LMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQLLRK 283
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Human
Match: CLN8 (CLN8 transmembrane ER and ERGIC protein [Source:HGNC Symbol;Acc:HGNC:2079])

HSP 1 Score: 167.548 bits (423), Expect = 4.104e-49
Identity = 90/257 (35.02%), Postives = 148/257 (57.59%), Query Frame = 2 
Query:  167 VIFASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIA------NGDVKRK 913
            ++ A  +  +GVF+    L S L  TYR L A+EK+FW +   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++  +V +   H+   + L+LEM+TPF+ + W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A      NG + RK
Sbjct:   27 LMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQLLRK 283
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Human
Match: CLN8 (CLN8 transmembrane ER and ERGIC protein [Source:HGNC Symbol;Acc:HGNC:2079])

HSP 1 Score: 167.548 bits (423), Expect = 4.104e-49
Identity = 90/257 (35.02%), Postives = 148/257 (57.59%), Query Frame = 2 
Query:  167 VIFASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIA------NGDVKRK 913
            ++ A  +  +GVF+    L S L  TYR L A+EK+FW +   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++  +V +   H+   + L+LEM+TPF+ + W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A      NG + RK
Sbjct:   27 LMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQLLRK 283
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Human
Match: CLN8 (CLN8 transmembrane ER and ERGIC protein [Source:HGNC Symbol;Acc:HGNC:2079])

HSP 1 Score: 167.548 bits (423), Expect = 4.104e-49
Identity = 90/257 (35.02%), Postives = 148/257 (57.59%), Query Frame = 2 
Query:  167 VIFASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIA------NGDVKRK 913
            ++ A  +  +GVF+    L S L  TYR L A+EK+FW +   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++  +V +   H+   + L+LEM+TPF+ + W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A      NG + RK
Sbjct:   27 LMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQLLRK 283
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Zebrafish
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:ZFIN;Acc:ZDB-GENE-060906-3])

HSP 1 Score: 155.992 bits (393), Expect = 4.760e-45
Identity = 91/240 (37.92%), Postives = 143/240 (59.58%), Query Frame = 2 
Query:  203 FIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVG-KMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNF--QNPIANGDVKRK 913
            F+   L + L +TYR L AKEK+FW +   RA+FG+ G   G+ A+     L +D + G+  +S   I  +  FF+FE++ + +S+  +  F + L +HH+ AL  +  AVV   + HF     L+LEM+TPF+ I W LLK   S T  WK NQ++++H+FH R V+  Y W+V   ++  + T +P    +L + GLFL+TF + P WT++KT QL+ P D+NF  Q+P  NG ++ +
Sbjct:   25 FLCHVLSTLLFQTYRSLSAKEKVFWDLAATRAVFGIQGIVAGLRALMEESVLFSDKILGQEDWSWFNILTSTGFFLFENMALHMSNVVFRSFDLPLAVHHFFALAGFAGAVVWNWQGHFLPMVTLLLEMSTPFTCISWMLLKAGWSKTVFWKANQWMMIHMFHCRMVVSYYMWWVCLNHWEEMNTHIPLPQRLLFFTGLFLLTFFLNPIWTHKKTLQLLNPVDWNFHNQSPPENGPLQEQ 264
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Zebrafish
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:ZFIN;Acc:ZDB-GENE-060906-3])

HSP 1 Score: 155.606 bits (392), Expect = 5.826e-45
Identity = 91/240 (37.92%), Postives = 143/240 (59.58%), Query Frame = 2 
Query:  203 FIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVG-KMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNF--QNPIANGDVKRK 913
            F+   L + L +TYR L AKEK+FW +   RA+FG+ G   G+ A+     L +D + G+  +S   I  +  FF+FE++ + +S+  +  F + L +HH+ AL  +  AVV   + HF     L+LEM+TPF+ I W LLK   S T  WK NQ++++H+FH R V+  Y W+V   ++  + T +P    +L + GLFL+TF + P WT++KT QL+ P D+NF  Q+P  NG ++ +
Sbjct:   25 FLCHVLSTLLFQTYRSLSAKEKVFWDLAATRAVFGIQGIVAGLRALMEESVLFSDKILGQEDWSWFNILTSTGFFLFENMALHMSNVVFRSFDLPLAVHHFFALAGFAGAVVWNWQGHFLPMVTLLLEMSTPFTCISWMLLKAGWSKTVFWKVNQWMMIHMFHCRMVVSYYMWWVCLNHWEEMNTHIPLPQRLLFFTGLFLLTFFLNPIWTHKKTLQLLNPVDWNFHNQSPPENGPLQEQ 264
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Zebrafish
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:ZFIN;Acc:ZDB-GENE-060906-3])

HSP 1 Score: 155.992 bits (393), Expect = 6.694e-45
Identity = 91/240 (37.92%), Postives = 143/240 (59.58%), Query Frame = 2 
Query:  203 FIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVG-KMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNF--QNPIANGDVKRK 913
            F+   L + L +TYR L AKEK+FW +   RA+FG+ G   G+ A+     L +D + G+  +S   I  +  FF+FE++ + +S+  +  F + L +HH+ AL  +  AVV   + HF     L+LEM+TPF+ I W LLK   S T  WK NQ++++H+FH R V+  Y W+V   ++  + T +P    +L + GLFL+TF + P WT++KT QL+ P D+NF  Q+P  NG ++ +
Sbjct:   42 FLCHVLSTLLFQTYRSLSAKEKVFWDLAATRAVFGIQGIVAGLRALMEESVLFSDKILGQEDWSWFNILTSTGFFLFENMALHMSNVVFRSFDLPLAVHHFFALAGFAGAVVWNWQGHFLPMVTLLLEMSTPFTCISWMLLKAGWSKTVFWKANQWMMIHMFHCRMVVSYYMWWVCLNHWEEMNTHIPLPQRLLFFTGLFLLTFFLNPIWTHKKTLQLLNPVDWNFHNQSPPENGPLQEQ 281
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Xenopus
Match: arpc3 (actin related protein 2/3 complex subunit 3 [Source:Xenbase;Acc:XB-GENE-494115])

HSP 1 Score: 178.718 bits (452), Expect = 2.167e-53
Identity = 99/265 (37.36%), Postives = 156/265 (58.87%), Query Frame = 2 
Query:  146 SFTENLIVIFASLISIIGVFIFSCLFSQLVK-TYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV--VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPI-------ANGDVKR 910
            S+   LI+I AS +  + +F+   L S L+  TY  L  KEK+FW +   RA+FG+     G+ A+     L  D + G+  +S  TI I + FF+FE+L + LS+F +    +LL +HH+ A + ++ AV+  +G  H+     L+LEM+TPF+ I W LLK   SDT  WK NQ+I++H+FH R V+  + W+V   N++     +PF ++   + GL ++T ++ PYWT++KTQQL+ P D+NF+          ANG +KR
Sbjct:   30 SWNTCLILIGASCLGYMFLFVLCHLVSILISATYCSLCPKEKVFWDLAATRAVFGIHCIYAGLRALLVDPVLTADTITGQQSWSQLTILIAVGFFLFENLALHLSNFIFWTCDILLAVHHFFAFLGFLGAVLYSIGG-HYLAMLTLLLEMSTPFTCISWMLLKAGCSDTLFWKVNQWIMIHMFHCRMVLTYHLWWVCLYNWDRFKNSLPFVYIAFFFTGLAILTIILNPYWTHKKTQQLLTPVDWNFKGTSVKSSSLKANGRIKR 293
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Mouse
Match: Cln8 (ceroid-lipofuscinosis, neuronal 8 [Source:MGI Symbol;Acc:MGI:1349447])

HSP 1 Score: 159.458 bits (402), Expect = 3.627e-46
Identity = 89/251 (35.46%), Postives = 145/251 (57.77%), Query Frame = 2 
Query:  176 ASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGET---WFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYV-TAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            A  +  +GVF+    L S L  TYR L AKEK+FW++   RA+FGV     G+WA+     L  D   G+    WF    I+    FF FE++ V LS+ ++  F + L +HH  A + ++ +A+ +   H+   + L+LEM+TPF+ I W LLK   SD+  WK NQ++++H+FH R ++  + W+V + +++++ + +      L   GL L+T ++ PYWT++KTQQL+ P D+NF    A G  + +
Sbjct:   30 AGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFH---ITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQER 277
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Mouse
Match: Cln8 (ceroid-lipofuscinosis, neuronal 8 [Source:MGI Symbol;Acc:MGI:1349447])

HSP 1 Score: 56.6102 bits (135), Expect = 1.049e-9
Identity = 38/98 (38.78%), Postives = 51/98 (52.04%), Query Frame = 2 
Query:  176 ASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGET---WFSHCTISITIAFFMFESLVVTLS 457
            A  +  +GVF+    L S L  TYR L AKEK+FW++   RA+FGV     G+WA+     L  D   G+    WF    I+    FF FE++ V LS
Sbjct:   30 AGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFH---ITTATGFFFFENVAVHLS 124
[Close]
BLAST of TLC domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9UBY8|CLN8_HUMAN (Protein CLN8 OS=Homo sapiens OX=9606 GN=CLN8 PE=1 SV=3)

HSP 1 Score: 167.548 bits (423), Expect = 1.971e-48
Identity = 90/257 (35.02%), Postives = 148/257 (57.59%), Query Frame = 2 
Query:  167 VIFASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIA------NGDVKRK 913
            ++ A  +  +GVF+    L S L  TYR L A+EK+FW +   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++  +V +   H+   + L+LEM+TPF+ + W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A      NG + RK
Sbjct:   27 LMVAGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWDLAATRAVFGVQSTAAGLWALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAFLGFLGCLVNLQAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFLVGLALLTLIINPYWTHKKTQQLLNPVDWNFAQPEAKSRPEGNGQLLRK 283
[Close]
BLAST of TLC domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6AYM9|CLN8_RAT (Protein CLN8 OS=Rattus norvegicus OX=10116 GN=Cln8 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 1.183e-46
Identity = 91/251 (36.25%), Postives = 149/251 (59.36%), Query Frame = 2 
Query:  176 ASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAI-GYSEDLNNDVVNGETWFSHCTISITIA--FFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYV-TAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            A  +  +GVF+    L S L  TYR L AKEK+FW++   RA+FG+     G+WA+ G     +N  +  + W   C   IT A  FF FE+  V LS+ ++  F + L +HH  A + ++ +AV +   H+   + L+LEM+TPF+ + W LLK   S +  WK NQ++++H+FH R ++  + W+V +++++++ + +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P A GD + +
Sbjct:   30 AGFVFYLGVFVVCHQLSSSLNATYRSLLAKEKVFWNLAATRAVFGIQSTAAGLWALLGDPVLYSNKALGQQNW---CWFHITTATGFFFFENAAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAVNLRAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSHSLFWKVNQWLMIHMFHCRMILTYHMWWVCFQHWDALASSLYLPHLALFLFGLALLTVIINPYWTHKKTQQLLNPVDWNFAQPEAKGDRQER 277
[Close]
BLAST of TLC domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5JZQ7|CLN8_CANLF (Protein CLN8 OS=Canis lupus familiaris OX=9615 GN=CLN8 PE=1 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 4.773e-46
Identity = 93/256 (36.33%), Postives = 148/256 (57.81%), Query Frame = 2 
Query:  176 ASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPI--------ANGDVKRK 913
            A  +  +GVF+    L S L  TYR L A+EK+FW++   RA+FGV     G+WA+     L+ D   G+  +    I+    FF FE++ V LS+  +  F + L +HH  A + ++ +VV +G  H+   S L+LE +TPF+ I W LLK   S++  WK NQ++++H+FH R V+  + W+V + +++ +++ +    L L   GL L+T ++ PYWT++KTQQL+ P D+NF  P         ANG V +K
Sbjct:   30 AGFVFYLGVFVVCHQLSSSLNATYRSLVAREKVFWNLAATRAVFGVQSTAAGLWALLVDPVLHADKARGQQNWCWFHIATATGFFFFENVAVHLSNVLFRTFDLFLAIHHLFAFLGFLGSVVNLGAGHYLAMSTLLLEASTPFTCISWMLLKAGWSESLFWKLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLALFLVGLGLLTLVINPYWTHKKTQQLLNPVDWNFAQPAPRSSRPAGANGQVPQK 285
[Close]
BLAST of TLC domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9QUK3|CLN8_MOUSE (Protein CLN8 OS=Mus musculus OX=10090 GN=Cln8 PE=1 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 2.537e-45
Identity = 89/251 (35.46%), Postives = 145/251 (57.77%), Query Frame = 2 
Query:  176 ASLISIIGVFIF-SCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGET---WFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYV-TAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            A  +  +GVF+    L S L  TYR L AKEK+FW++   RA+FGV     G+WA+     L  D   G+    WF    I+    FF FE++ V LS+ ++  F + L +HH  A + ++ +A+ +   H+   + L+LEM+TPF+ I W LLK   SD+  WK NQ++++H+FH R ++  + W+V + +++++ + +      L   GL L+T ++ PYWT++KTQQL+ P D+NF    A G  + +
Sbjct:   30 AGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFH---ITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQER 277
[Close]
BLAST of TLC domain-containing protein vs. TrEMBL
Match: A0A210QHV3 (Protein CLN8 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT11830 PE=4 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 1.380e-78
Identity = 125/258 (48.45%), Postives = 170/258 (65.89%), Query Frame = 2 
Query:  152 TENLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQ----NPIANGDVKRK 913
            +  L  I AS I    V++ S +F  L KTYR LK+KEKIFW++ +VR ++G F   VGVWAI +  +L  D V   T  S+  ++ ++ FF+FE   + +SD  Y    V+LN+HHW+AL+ +V  + +   H+FG  GL+LEM+TPFSAICWTLLK   + T LWK NQF+LVH FH+RSV+E Y WYVTY+N+  I + +P    + +Y  L LVTFLMTPYWTY+KTQQ+I P D+NF+    N   NG V +K
Sbjct:   20 STKLFFICASFIFFGTVYLLSAVFGSLTKTYRNLKSKEKIFWNLAVVRGVYGSFCTVVGVWAIFFDTELLKDPVFSTTPTSYFALATSLGFFVFECGTLLISDMVYKKASVMLNVHHWMALLGFVLLMYLDSAHYFGVRGLLLEMSTPFSAICWTLLKASKAHTLLWKANQFLLVHTFHLRSVVECYIWYVTYQNWERIWSAMPTTMFISLYSQLVLVTFLMTPYWTYKKTQQMITPVDWNFEDSGKNKSENGSVSKK 277
[Close]
BLAST of TLC domain-containing protein vs. TrEMBL
Match: K1PTP9 (Protein CLN8 OS=Crassostrea gigas OX=29159 GN=CGI_10018253 PE=4 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 4.775e-75
Identity = 116/259 (44.79%), Postives = 167/259 (64.48%), Query Frame = 2 
Query:  146 SFTENLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQ----NPIANGDVKR 910
             +T  +  IFAS +    +++ + L S    TYR L+ KEK+FW + +VRA++G+F   VGVWAI    +L  D V   +  S+  +++T+ FF+FE   + +SD  Y    +LLN+HHWL+L+ Y T +V    H FG  GL+LEM+TPFSAICWT+LK   +D+  W  NQF+LVH FH+RSV+E + WY+TY+++  I + +P      +Y  L LVTFLMTPYWTY+KTQQ+I P D+NF+    N   NG+ K+
Sbjct:   18 KWTVKVTFIFASFLFFAALYLLTGLVSMTTWTYRNLREKEKMFWKLAVVRAMYGIFCTVVGVWAIFVDTELEKDPVFATSPTSYFALTVTVGFFVFECGAILISDIVYRKVSILLNIHHWLSLLGYSTLMVTEAAHCFGTKGLILEMSTPFSAICWTVLKAGKADSLFWHANQFMLVHTFHLRSVVECFMWYLTYQHWERIWSAMPLSIFSFLYIQLILVTFLMTPYWTYKKTQQMITPVDWNFEDSEANKARNGEAKK 276
[Close]
BLAST of TLC domain-containing protein vs. TrEMBL
Match: A0A2T7NTJ6 (TLC domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_14974 PE=4 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 2.589e-72
Identity = 114/245 (46.53%), Postives = 160/245 (65.31%), Query Frame = 2 
Query:  200 VFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQN-------PIANGDVKRK 913
            VF+ S + +    TY+ L+ KEKIFW++ +VRA FG F   VG WAI    +++ DVV G T  SH  + +T+ FF+FE   +TLSD ++  F  LLNLHHWL+L+ Y   + +   H FGC GL+LEM+TPFSA+CWTLLK     + LWK NQF+LVH FH+R+V+E  FWY+TY+N+  I   +P    + +Y  L LV+ +MTPYWTY+KT Q+I P D+NF++         ANG+ + +
Sbjct:   54 VFVLSAIVASATLTYQKLRLKEKIFWNLALVRASFGFFSMFVGAWAIFTHTEMDKDVVFGTTSTSHFALLVTVGFFLFECGALTLSDIFFKSFSFLLNLHHWLSLVGYSLVIYIEAGHCFGCKGLILEMSTPFSALCWTLLKAGKERSLLWKANQFLLVHAFHLRNVVECLFWYITYQNWTYIRENMPTPIFLALYVQLTLVSLIMTPYWTYKKTVQMINPIDWNFESQKRPRNMSYANGNAETE 298
[Close]
BLAST of TLC domain-containing protein vs. TrEMBL
Match: A0A1S3K4K2 (protein CLN8 OS=Lingula unguis OX=7574 GN=LOC106178704 PE=4 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 1.431e-70
Identity = 118/257 (45.91%), Postives = 164/257 (63.81%), Query Frame = 2 
Query:  158 NLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGET---WFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQN----PIANGDVK 907
             L +I +S +    V++ S   + +  TYR L+ KEK+FW++++VRA+FGV+  C GVWA+ +  +L  DVV   T   WFS C   + + FF+FE   + +SD  +     LLNLHHWLAL+ YV  +V    HFFG  GL+LEM+TPFSA+CWTLLKC +SDT LWK NQF+LVH FH RS++E Y W  +Y+N+  I   +P      +Y  L L+ F+MTPYWTY+KT QL+   D+NF++       NG +K
Sbjct:   22 QLEIIGSSFLFFFVVYLASAFIASMTWTYRNLRIKEKVFWNLSVVRAVFGVYSTCAGVWAVWWDTELKEDVVFATTPTSWFSLC---LCVGFFLFECSAMFVSDVIFRQANPLLNLHHWLALLGYVLVMVYRSSHFFGTVGLLLEMSTPFSALCWTLLKCGLSDTLLWKLNQFVLVHTFHCRSLLECYLWLQSYRNWERIWQAMPTPIFTALYVQLTLIFFIMTPYWTYKKTVQLVNHRDWNFEDTETTKETNGSLK 275
[Close]
BLAST of TLC domain-containing protein vs. TrEMBL
Match: A0A210QHQ7 (Protein CLN8 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT11829 PE=4 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 2.154e-70
Identity = 117/240 (48.75%), Postives = 155/240 (64.58%), Query Frame = 2 
Query:  206 IFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQ----NPIANGDVKRK 913
            + S     L KTYR L+ KEKIFW++ +VR  +G+F   VG+WAI Y  +L  D V   T  S+  ++ T+ FF+FE   + +SDF +    VLLN+HHWL+L  Y   + +   H FG  GL+LEM+TPFS ICWTLLK   +D+ LWK NQF LVH FH+RSV E Y WY+TY+N+  I + +P    + +Y  L LVTFLMTPYWTY+KTQQ+I P D+NF+    N   NG V +K
Sbjct:   38 VVSGTLGSLTKTYRNLRQKEKIFWNLAVVRGAYGMFCIVVGIWAIFYDTELVKDPVFSTTPTSYFALTTTVGFFVFECGTLLISDFVFKKASVLLNIHHWLSLSGYSLLLYLDSTHCFGTKGLILEMSTPFSGICWTLLKAGQADSLLWKANQFFLVHTFHLRSVAECYLWYLTYQNWERIWSAMPTAMFISLYTQLVLVTFLMTPYWTYKKTQQMITPVDWNFEDSNKNKSQNGSVTKK 277
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Cavefish
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:NCBI gene;Acc:103023421])

HSP 1 Score: 162.155 bits (409), Expect = 2.191e-47
Identity = 88/235 (37.45%), Postives = 139/235 (59.15%), Query Frame = 2 
Query:  218 LFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV---VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            L + L  TYR L AKEK+FW +   RA+FGV  A  G+ A+     + ++ V G+T +S   +   ++FF+FE++ +  S   +  F + L +HH  AL  +  AVV   VG  HF     L+LEM+TPF+ I W LLK   + T  WK NQ++++H+FH R V+  Y W+V++ ++  +   +P   L++ + GL L+TFL+ P WT++KT QL+ P D+NF N   + +  R+
Sbjct:   50 LSTTLSLTYRSLPAKEKVFWDLAATRAMFGVQSAVAGLRALIEDGPVFDEKVTGQTGWSWFNVLTAVSFFLFENVALHSSSVAFRSFDLPLAVHHAFALAGFAGAVVWEHVG--HFLPMVTLLLEMSTPFTCISWMLLKAGWARTLFWKANQWLMIHMFHCRMVLTYYMWWVSWSHWTDLSIHIPLPQLLVFFSGLALLTFLINPIWTHKKTMQLLNPVDWNFANKPTSENGPRE 282
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Sea Lamprey
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:ZFIN;Acc:ZDB-GENE-060906-3])

HSP 1 Score: 164.081 bits (414), Expect = 7.953e-49
Identity = 88/240 (36.67%), Postives = 137/240 (57.08%), Query Frame = 2 
Query:  200 VFIFSCLFSQ-LVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKM-HFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            VF+ + L SQ L  TY+ L ++E+ FW +   RA+FGV     G+W +     L   +V  + W+S   I  T+ FF+FE+L + +S   +GG  + L +HH L+   ++T V+     H+     L+LEM+TPF+ I W LLK   + T LW+ NQ +++H+FH R  +  Y W+ +++N   ++  V    L++ Y GL ++T  + PYWTY+KT QL  P D+NF    AN DV R 
Sbjct:   39 VFLITHLVSQALSVTYKSLHSQERAFWSLAATRAVFGVQATFAGLWTLLADRWLFEGLVWRQCWWSWALILTTVGFFLFENLALHVSSAVFGGLDLGLAVHHALSAGGFLTMVLWDSTGHYLAIVILLLEMSTPFTCISWMLLKAGYAKTLLWRVNQMVMIHMFHGRMFLTYYLWWESWRNRQDLVNHVSLPTLVIFYTGLLVLTVYLNPYWTYKKTLQLANPVDWNF----ANKDVNRN 274
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Sea Lamprey
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:ZFIN;Acc:ZDB-GENE-060906-3])

HSP 1 Score: 163.696 bits (413), Expect = 1.444e-48
Identity = 88/240 (36.67%), Postives = 137/240 (57.08%), Query Frame = 2 
Query:  200 VFIFSCLFSQ-LVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKM-HFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            VF+ + L SQ L  TY+ L ++E+ FW +   RA+FGV     G+W +     L   +V  + W+S   I  T+ FF+FE+L + +S   +GG  + L +HH L+   ++T V+     H+     L+LEM+TPF+ I W LLK   + T LW+ NQ +++H+FH R  +  Y W+ +++N   ++  V    L++ Y GL ++T  + PYWTY+KT QL  P D+NF    AN DV R 
Sbjct:   39 VFLITHLVSQALSVTYKGLHSQERAFWSLAATRAVFGVQATFAGLWTLLADRWLFEGLVWRQCWWSWALILTTVGFFLFENLALHVSSAVFGGLDLGLAVHHALSAGGFLTMVLWDSTGHYLAIVILLLEMSTPFTCISWMLLKAGYAKTLLWRVNQMVMIHMFHGRMFLTYYLWWESWRNRQDLVNHVSLPTLVIFYTGLLVLTVYLNPYWTYKKTLQLANPVDWNF----ANKDVNRN 274
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Medaka
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:NCBI gene;Acc:101156618])

HSP 1 Score: 160.999 bits (406), Expect = 7.254e-47
Identity = 80/222 (36.04%), Postives = 128/222 (57.66%), Query Frame = 2 
Query:  236 KTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQN-PIAN 895
            +TY  L +KEK+FW++   RA FG+  +  G+ A+    DL+ D V G+  +S   +     FF FE++ +  S   +G F + L  HH+ AL  +  AVV   + HF     L+LEM+TPF+ I W LLK   + T  W+ NQ++++H+FH R V+  Y W++   +++ + T V     +L + GL L+T ++ P WT++KT QL+ P D+NF N P+  
Sbjct:   46 QTYNSLLSKEKVFWNLAATRAAFGIQSSVAGLRALTEDSDLSRDKVRGQEDWSWFHLLTATGFFFFENVALHTSSVVFGSFDIPLATHHFFALAGFAGAVVWDSQGHFLAMVTLLLEMSTPFTCISWMLLKAGWARTLFWRANQWVMIHMFHCRMVLTYYMWWIAVTHWSELSTYVALSQRLLFFSGLALLTLIINPIWTHKKTMQLLNPVDWNFDNKPVPE 267
[Close]
BLAST of TLC domain-containing protein vs. Ensembl Medaka
Match: cln8 (CLN8 transmembrane ER and ERGIC protein [Source:NCBI gene;Acc:101156618])

HSP 1 Score: 160.999 bits (406), Expect = 3.794e-46
Identity = 80/222 (36.04%), Postives = 128/222 (57.66%), Query Frame = 2 
Query:  236 KTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVV-VGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQN-PIAN 895
            +TY  L +KEK+FW++   RA FG+  +  G+ A+    DL+ D V G+  +S   +     FF FE++ +  S   +G F + L  HH+ AL  +  AVV   + HF     L+LEM+TPF+ I W LLK   + T  W+ NQ++++H+FH R V+  Y W++   +++ + T V     +L + GL L+T ++ P WT++KT QL+ P D+NF N P+  
Sbjct:  112 QTYNSLLSKEKVFWNLAATRAAFGIQSSVAGLRALTEDSDLSRDKVRGQEDWSWFHLLTATGFFFFENVALHTSSVVFGSFDIPLATHHFFALAGFAGAVVWDSQGHFLAMVTLLLEMSTPFTCISWMLLKAGWARTLFWRANQWVMIHMFHCRMVLTYYMWWIAVTHWSELSTYVALSQRLLFFSGLALLTLIINPIWTHKKTMQLLNPVDWNFDNKPVPE 333
[Close]
BLAST of TLC domain-containing protein vs. Planmine SMEST
Match: SMESG000026561.1 (SMESG000026561.1)

HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0
Identity = 257/258 (99.61%), Postives = 258/258 (100.00%), Query Frame = 2 
Query:  140 MFSFTENLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 913
            MFSFTENLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMS+TKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK
Sbjct:    1 MFSFTENLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTIVRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFESLVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMTTPFSAICWTLLKCRMSETKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNYNSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPIANGDVKRK 258
The following BLAST results are available for this feature:
BLAST of TLC domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CLN84.104e-4935.02CLN8 transmembrane ER and ERGIC protein [Source:HG... [more]
CLN84.104e-4935.02CLN8 transmembrane ER and ERGIC protein [Source:HG... [more]
CLN84.104e-4935.02CLN8 transmembrane ER and ERGIC protein [Source:HG... [more]
CLN84.104e-4935.02CLN8 transmembrane ER and ERGIC protein [Source:HG... [more]
CLN84.104e-4935.02CLN8 transmembrane ER and ERGIC protein [Source:HG... [more]
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BLAST of TLC domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TLC domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TLC domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cln84.760e-4537.92CLN8 transmembrane ER and ERGIC protein [Source:ZF... [more]
cln85.826e-4537.92CLN8 transmembrane ER and ERGIC protein [Source:ZF... [more]
cln86.694e-4537.92CLN8 transmembrane ER and ERGIC protein [Source:ZF... [more]
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BLAST of TLC domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
arpc32.167e-5337.36actin related protein 2/3 complex subunit 3 [Sourc... [more]
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BLAST of TLC domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Cln83.627e-4635.46ceroid-lipofuscinosis, neuronal 8 [Source:MGI Symb... [more]
Cln81.049e-938.78ceroid-lipofuscinosis, neuronal 8 [Source:MGI Symb... [more]
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BLAST of TLC domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9UBY8|CLN8_HUMAN1.971e-4835.02Protein CLN8 OS=Homo sapiens OX=9606 GN=CLN8 PE=1 ... [more]
sp|Q6AYM9|CLN8_RAT1.183e-4636.25Protein CLN8 OS=Rattus norvegicus OX=10116 GN=Cln8... [more]
sp|Q5JZQ7|CLN8_CANLF4.773e-4636.33Protein CLN8 OS=Canis lupus familiaris OX=9615 GN=... [more]
sp|Q9QUK3|CLN8_MOUSE2.537e-4535.46Protein CLN8 OS=Mus musculus OX=10090 GN=Cln8 PE=1... [more]
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BLAST of TLC domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QHV31.380e-7848.45Protein CLN8 OS=Mizuhopecten yessoensis OX=6573 GN... [more]
K1PTP94.775e-7544.79Protein CLN8 OS=Crassostrea gigas OX=29159 GN=CGI_... [more]
A0A2T7NTJ62.589e-7246.53TLC domain-containing protein OS=Pomacea canalicul... [more]
A0A1S3K4K21.431e-7045.91protein CLN8 OS=Lingula unguis OX=7574 GN=LOC10617... [more]
A0A210QHQ72.154e-7048.75Protein CLN8 OS=Mizuhopecten yessoensis OX=6573 GN... [more]
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BLAST of TLC domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cln82.191e-4737.45CLN8 transmembrane ER and ERGIC protein [Source:NC... [more]
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BLAST of TLC domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cln87.953e-4936.67CLN8 transmembrane ER and ERGIC protein [Source:ZF... [more]
cln81.444e-4836.67CLN8 transmembrane ER and ERGIC protein [Source:ZF... [more]
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BLAST of TLC domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TLC domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TLC domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cln87.254e-4736.04CLN8 transmembrane ER and ERGIC protein [Source:NC... [more]
cln83.794e-4636.04CLN8 transmembrane ER and ERGIC protein [Source:NC... [more]
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BLAST of TLC domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000026561.10.000e+099.61SMESG000026561.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019291 ID=SMED30019291|Name=TLC domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1012bp
ATAAAGTTCTGTGCGTATTCAGTTCAACGTCATCGGCTGAAATGATCTAC
ATTCCAAGTTGCATCATATGATTATATAATTTGATAAATTAGTAATAAAA
GTGATGGATGACAATAAATAATTTATTGAAATTTAGTAAATGTTCTCCTT
TACTGAAAACTTAATTGTGATATTCGCTTCACTCATATCAATTATTGGAG
TCTTCATATTTTCATGTCTTTTCAGTCAGCTAGTGAAAACCTACAGATGC
TTGAAAGCGAAAGAGAAAATATTCTGGCACATGACAATCGTGAGAGCTTT
ATTTGGTGTCTTTGGGGCTTGCGTTGGAGTCTGGGCTATCGGATATTCAG
AAGACCTCAACAATGATGTCGTGAATGGAGAAACGTGGTTCAGTCATTGC
ACCATATCGATAACGATTGCGTTTTTCATGTTTGAATCTTTAGTGGTGAC
ATTATCAGATTTTTATTACGGTGGTTTCCAGGTTTTGTTGAACCTTCACC
ATTGGCTCGCGCTGATGAACTATGTGACCGCCGTTGTAGTTGGGAAAATG
CATTTTTTCGGTTGCAGTGGCCTGGTTTTAGAAATGACTACTCCATTTTC
AGCGATCTGTTGGACTTTGCTCAAATGTCGAATGAGTGATACAAAACTCT
GGAAAACTAATCAGTTTATCCTTGTTCATTTGTTTCACGTGAGATCGGTC
ATTGAAGTATATTTCTGGTATGTCACTTACAAAAATTATAATTCAATAAT
CACTCAAGTTCCTTTTTACTTTCTTATGCTCATCTACGGTGGTTTGTTTT
TAGTGACCTTCCTTATGACTCCCTATTGGACTTACAGAAAGACCCAACAA
CTTATCATGCCATTTGATTTCAATTTTCAGAATCCAATAGCAAATGGAGA
TGTAAAAAGAAAATAAATGAATCCTAACATATATAAATGTTCTTTCGTTT
TAGCCTTTATAACTGATGTTTATTTCACATAGAGAGAATATATTGTTTTT
ACTTAAAAAAAA
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protein sequence of SMED30019291-orf-1

>SMED30019291-orf-1 ID=SMED30019291-orf-1|Name=SMED30019291-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=259bp
MFSFTENLIVIFASLISIIGVFIFSCLFSQLVKTYRCLKAKEKIFWHMTI
VRALFGVFGACVGVWAIGYSEDLNNDVVNGETWFSHCTISITIAFFMFES
LVVTLSDFYYGGFQVLLNLHHWLALMNYVTAVVVGKMHFFGCSGLVLEMT
TPFSAICWTLLKCRMSDTKLWKTNQFILVHLFHVRSVIEVYFWYVTYKNY
NSIITQVPFYFLMLIYGGLFLVTFLMTPYWTYRKTQQLIMPFDFNFQNPI
ANGDVKRK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR006634TLC-dom
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006634TRAM/LAG1/CLN8 homology domainSMARTSM00724lag1_27coord: 44..243
e-value: 0.0057
score: 10.8
IPR006634TRAM/LAG1/CLN8 homology domainPFAMPF03798TRAM_LAG1_CLN8coord: 47..232
e-value: 7.6E-8
score: 32.3
IPR006634TRAM/LAG1/CLN8 homology domainPROSITEPS50922TLCcoord: 41..243
score: 16.214
NoneNo IPR availablePANTHERPTHR13439:SF7PROTEIN CLN8coord: 8..252
NoneNo IPR availablePANTHERPTHR13439CT120 PROTEINcoord: 8..252
NoneNo IPR availableTMHMMTMhelixcoord: 45..67
NoneNo IPR availableTMHMMTMhelixcoord: 210..232
NoneNo IPR availableTMHMMTMhelixcoord: 82..104
NoneNo IPR availableTMHMMTMhelixcoord: 10..32
NoneNo IPR availableTMHMMTMhelixcoord: 173..195
NoneNo IPR availableTMHMMTMhelixcoord: 139..161
NoneNo IPR availableTMHMMTMhelixcoord: 116..135