FHA domain-containing protein

Overview


Name	FHA domain-containing protein
Smed ID	SMED30019275
Length (bp)	1810

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of FHA domain-containing protein (SMED30019275) t-SNE clustered cells

Violin plots show distribution of expression levels for FHA domain-containing protein (SMED30019275) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of FHA domain-containing protein (SMED30019275) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for FHA domain-containing protein (SMED30019275) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019275

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parenchyma	SMED30019275	h1SMcG0022239	dd_Smed_v4_13269_0_1	dd_Smed_v4	PMID:27063937 Scimone et al., 2016
pigment cell	SMED30019275	h1SMcG0022239	SmedASXL_016771	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
X1 cell	SMED30019275	h1SMcG0022239	SmedASXL_016771	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30019275	h1SMcG0022239	SmedASXL_016771	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
cephalic ganglia	SMED30019275	h1SMcG0022239	dd_Smed_v4_13269_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30019275	h1SMcG0022239	dd_Smed_v4_13269_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30019275	h1SMcG0022239	dd_Smed_v6_13269_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
body wall musculature	SMED30019275	h1SMcG0022239	dd_Smed_v4_13269_0_1	dd_Smed_v4	PMID:30471994 Scimone et al., 2018
zeta neoblast	SMED30019275	h1SMcG0022239	dd_Smed_v4_13269_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
neoblast	SMED30019275	h1SMcG0022239	SMED30019275	smed_20140614	PMID:27523733 Lei et al., 2016
neoblast	SMED30019275	h1SMcG0022239	SMED_09673_V2_1	GPL14150_gene_models	PMID:22385657 Wagner et al., 2012
neoblast	SMED30019275	h1SMcG0022239	SMED_09673_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012

Note: Hover over icons to view figure legend

Homology

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BLAST of FHA domain-containing protein vs. Ensembl Human
Match: NBN (nibrin [Source:HGNC Symbol;Acc:HGNC:7652])

HSP 1 Score: 110.538 bits (275), Expect = 1.335e-24
Identity = 87/317 (27.44%), Postives = 143/317 (45.11%), Query Frame = 1

Query:   94 FLPSVTYTIGRVSADVILTH-TSISRKHAVLIWEC---------DLNYITIDDCSKFGTFINKAPLINK-KGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLVNEISLI--------------LKGINKRLINDSEIGFAVMLASVDEYCNP 969
             L  V Y +GR +  +++ +  SISR HAVL             ++  +T+ D SK+GTF+N+  + N     +K GD +TFG   G      +       S L  + +  L Q +  +GG  + N  ++  ++VM ++K T K +CALI    IVK ++    +   +   Q  P  + + P +  P      +D     +RK +F  K F FL  ++   LS AV+ GGG A L+T+ +  + +  +    C        V T + N  +LI              L+    R I ++EIG AV+  +   YC+P
Sbjct:   18 LLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKDNSKYGTFVNEEKMQNGFSRTLKSGDGITFGVF-GSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQP-PQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTC-------VVDTGITNSQTLIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDP 325

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Human
Match: NBN (nibrin [Source:HGNC Symbol;Acc:HGNC:7652])

HSP 1 Score: 84.7297 bits (208), Expect = 1.510e-16
Identity = 65/244 (26.64%), Postives = 107/244 (43.85%), Query Frame = 1

Query:  280 IKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLVNEISLI--------------LKGINKRLINDSEIGFAVMLASVDEYCNP 969
            +K GD +TFG   G      +       S L  + +  L Q +  +GG  + N  ++  ++VM ++K T K +CALI    IVK ++    +   +   Q  P  + + P +  P      +D     +RK +F  K F FL  ++   LS AV+ GGG A L+T+ +  + +  +    C        V T + N  +LI              L+    R I ++EIG AV+  +   YC+P
Sbjct:    9 LKSGDGITFGVF-GSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQP-PQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTC-------VVDTGITNSQTLIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDP 243

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Human
Match: NBN (nibrin [Source:HGNC Symbol;Acc:HGNC:7652])

HSP 1 Score: 62.003 bits (149), Expect = 1.069e-10
Identity = 44/159 (27.67%), Postives = 72/159 (45.28%), Query Frame = 1

Query:  277 NIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGG 753
             +K GD +TFG   G      +       S L  + +  L Q +  +GG  + N  ++  ++VM ++K T K +CALI    IVK ++    +   +   Q  P  + + P +  P      +D     +RK +F  K F FL  ++   LS AV+ GG
Sbjct:    8 TLKSGDGITFGVF-GSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQ-PPQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGG 164

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BLAST of FHA domain-containing protein vs. Ensembl Fly
Match: nbs (gene:FBgn0261530 transcript:FBtr0306039)

HSP 1 Score: 100.908 bits (250), Expect = 8.849e-22
Identity = 80/309 (25.89%), Postives = 142/309 (45.95%), Query Frame = 1

Query:  112 YTIGRVSADVILTHT-SISRKHAVLIWECDLN---YITIDDC-SKFGTFI--------NKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGIN--QILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVK---LAHCDSIVMQ-----EAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNP 969
            YTIGR++ D+I+    SISR HA L+ + + +    + I+D  S++GTFI         K P       +  G  + FGA   +    + +      S L+ ++   L + L  +GG +  N  ++  ++ M+ +  T K++ A+++   IV   + + M+   + I+  +  P P+ Y P           +D    P+R  LF  K F F+ ++   +    V   G          C+D +  V+   L   D IV+Q     ++  T  +N I  IL+   +R+I + EIG A++  S+ E+CNP
Sbjct:   20 YTIGRLATDLIVAQDLSISRNHAQLLIQTEADGDDTLHIEDLGSRYGTFIFPKNSQKPRKVPA-KTSTPLPVGTRLRFGANMSI-WQVTQLKLVTTVSALTRSEVQELTKMLEPMGGTVTSNWTEECSHLTMNEVSVTVKLLHAMLENKPIVTFPYWRKMLQAAQSIHVKEGWPQPEDYQP---------TNIDVTWRPERTRLFAGKTFVFMNRKHFDMYGSVVQKAGAT--------CKDINSGVRKTFLTKSDVIVIQYVPSSQSQATESINSIQDILEQNGRRIIQEYEIGMALIHCSITEFCNP 309

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Fly
Match: nbs (gene:FBgn0261530 transcript:FBtr0306040)

HSP 1 Score: 97.0561 bits (240), Expect = 1.366e-20
Identity = 80/311 (25.72%), Postives = 142/311 (45.66%), Query Frame = 1

Query:  112 YTIGRVSADVILTHT-SISRKHAVLIWECDLN---YITIDDC-SKFGTFI--------NKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGIN--QILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVK---LAHCDSIVMQ-----EAVTTNLVNEIS--LILKGINKRLINDSEIGFAVMLASVDEYCNP 969
            YTIGR++ D+I+    SISR HA L+ + + +    + I+D  S++GTFI         K P       +  G  + FGA   +    + +      S L+ ++   L + L  +GG +  N  ++  ++ M+ +  T K++ A+++   IV   + + M+   + I+  +  P P+ Y P           +D    P+R  LF  K F F+ ++   +    V   G          C+D +  V+   L   D IV+Q     ++  T  +N I    IL+   +R+I + EIG A++  S+ E+CNP
Sbjct:   20 YTIGRLATDLIVAQDLSISRNHAQLLIQTEADGDDTLHIEDLGSRYGTFIFPKNSQKPRKVPA-KTSTPLPVGTRLRFGANMSI-WQVTQLKLVTTVSALTRSEVQELTKMLEPMGGTVTSNWTEECSHLTMNEVSVTVKLLHAMLENKPIVTFPYWRKMLQAAQSIHVKEGWPQPEDYQP---------TNIDVTWRPERTRLFAGKTFVFMNRKHFDMYGSVVQKAGAT--------CKDINSGVRKTFLTKSDVIVIQYVPSSQSQATESINSIQDRYILEQNGRRIIQEYEIGMALIHCSITEFCNP 311

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Zebrafish
Match: nbn (nibrin [Source:NCBI gene;Acc:544655])

HSP 1 Score: 137.887 bits (346), Expect = 1.286e-33
Identity = 103/337 (30.56%), Postives = 161/337 (47.77%), Query Frame = 1

Query:   55 WKF---ENKNKNIVKYFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDP--DHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTN-----------LVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKEN 1005
            WK    E+  ++++   L    Y +GR + +++LT+  SISR HAVL        +T+ D SK+GTF+N   L       ++ G  +TFG  Q    L    +   +VCS+ + N  +V L Q +  +GG L+ +      ++VM  +K T K +CAL+    IVK  F  A+    K + Q LP P  + + P+I  P      +D    P+RK LF  K F FL+ +++  LS AV  GGG++ LL +      +L V L    S  + + ++ N            ++ +  IL     R I +SE+G A +  S   YCNP  +L     K N
Sbjct:    2 WKLQPTESGGESVI--LLAGQEYVVGRKNCEILLTNDQSISRVHAVLT--VTEQAVTLKDSSKYGTFVNGEKLESGSTKTLQTGYKITFGVFQSKFSLEKECI---VVCSSCVDNEGKVTLSQDIRSVGGRLVSSWTSDCTHLVMPTVKVTIKTICALLCCRPIVKPAFFSALS---KAVQQKLPLPKAERFRPQIDEPSLARDDVDLSARPERKSLFKGKTFLFLSSKQMKRLSVAVSCGGGVSQLLDEG-----ALPVSLLESSSTCVLDMISGNSQPVISPASKKWLDSVGQILHRKGLRFITESEVGLAAIHVSNQTYCNPCSSLQSESVKTN 323

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Zebrafish
Match: nbn (nibrin [Source:NCBI gene;Acc:544655])

HSP 1 Score: 137.887 bits (346), Expect = 1.371e-33
Identity = 103/337 (30.56%), Postives = 161/337 (47.77%), Query Frame = 1

Query:   55 WKF---ENKNKNIVKYFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDP--DHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTN-----------LVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKEN 1005
            WK    E+  ++++   L    Y +GR + +++LT+  SISR HAVL        +T+ D SK+GTF+N   L       ++ G  +TFG  Q    L    +   +VCS+ + N  +V L Q +  +GG L+ +      ++VM  +K T K +CAL+    IVK  F  A+    K + Q LP P  + + P+I  P      +D    P+RK LF  K F FL+ +++  LS AV  GGG++ LL +      +L V L    S  + + ++ N            ++ +  IL     R I +SE+G A +  S   YCNP  +L     K N
Sbjct:    2 WKLQPTESGGESVI--LLAGQEYVVGRKNCEILLTNDQSISRVHAVLT--VTEQAVTLKDSSKYGTFVNGEKLESGSTKTLQTGYKITFGVFQSKFSLEKECI---VVCSSCVDNEGKVTLSQDIRSVGGRLVSSWTSDCTHLVMPTVKVTIKTICALLCCRPIVKPAFFSALS---KAVQQKLPLPKAERFRPQIDEPSLARDDVDLSARPERKSLFKGKTFLFLSSKQMKRLSVAVSCGGGVSQLLDEG-----ALPVSLLESSSTCVLDMISGNSQPVISPASKKWLDSVGQILHRKGLRFITESEVGLAAIHVSNQTYCNPCSSLQSESVKTN 323

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Zebrafish
Match: nbn (nibrin [Source:NCBI gene;Acc:544655])

HSP 1 Score: 137.502 bits (345), Expect = 1.680e-33
Identity = 103/337 (30.56%), Postives = 161/337 (47.77%), Query Frame = 1

Query:   55 WKF---ENKNKNIVKYFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDP--DHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTN-----------LVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKEN 1005
            WK    E+  ++++   L    Y +GR + +++LT+  SISR HAVL        +T+ D SK+GTF+N   L       ++ G  +TFG  Q    L    +   +VCS+ + N  +V L Q +  +GG L+ +      ++VM  +K T K +CAL+    IVK  F  A+    K + Q LP P  + + P+I  P      +D    P+RK LF  K F FL+ +++  LS AV  GGG++ LL +      +L V L    S  + + ++ N            ++ +  IL     R I +SE+G A +  S   YCNP  +L     K N
Sbjct:    2 WKLQPTESGGESVI--LLAGQEYVVGRKNCEILLTNDQSISRVHAVLT--VTEQAVTLKDSSKYGTFVNGEKLESGSTKTLQTGYKITFGVFQSKFSLEKECI---VVCSSCVDNEGKVTLSQDIRSVGGRLVSSWTSDCTHLVMPTVKVTIKTICALLCCRPIVKPAFFSALS---KAVQQKLPLPKAERFRPQIDEPSLARDDVDLSARPERKSLFKGKTFLFLSSKQMKRLSVAVSCGGGVSQLLDEG-----ALPVSLLESSSTCVLDMISGNSQPVISPASKKWLDSVGQILHRKGLRFITESEVGLAAIHVSNQTYCNPCSSLQSESVKTN 323

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Zebrafish
Match: nbn (nibrin [Source:NCBI gene;Acc:544655])

HSP 1 Score: 60.4622 bits (145), Expect = 2.005e-10
Identity = 47/154 (30.52%), Postives = 78/154 (50.65%), Query Frame = 1

Query:   55 WKF---ENKNKNIVKYFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKV 495
            WK    E+  ++++   L    Y +GR + +++LT+  SISR HAVL        +T+ D SK+GTF+N   L       ++ G  +TFG  Q    L    +   +VCS+ + N  +V L Q +  +GG L+ +      ++VM  +K T K+
Sbjct:    2 WKLQPTESGGESVI--LLAGQEYVVGRKNCEILLTNDQSISRVHAVLT--VTEQAVTLKDSSKYGTFVNGEKLESGSTKTLQTGYKITFGVFQSKFSLEKECI---VVCSSCVDNEGKVTLSQDIRSVGGRLVSSWTSDCTHLVMPTVKVTIKI 148

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Xenopus
Match: adcy9 (adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-999257])

HSP 1 Score: 124.02 bits (310), Expect = 5.387e-29
Identity = 100/319 (31.35%), Postives = 162/319 (50.78%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWEC---------DLNYITIDDCSKFGTFINKA---PLINKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDH--YLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAML---LTDSDCRDQSLR--VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYR 975
            Y L    Y +GR +  +++    SISR HA L              ++ +TI D SK+GT ++     P + +  ++K  D V FG       +  + V ++  +VCS+ L N ++  LKQ L  +GG++L N  +K  ++VM+++K T K +CALI    I+K D+ + +++    I Q  P P++  ++P I  P      LD   N KRK +F +K F FL  ++   LS AVL GGG A L   LTD+   + S    + +A  ++ + +   T   ++    +L     R I ++EIG AV+  S + YCNP R
Sbjct:   15 YILTGTDYVVGRKNCAILIPDDQSISRCHATLSVSYPSASLSQTNAVSVLTIKDSSKYGTTVSGERMQPAVPR--SLKPSDEVIFGC-----FHSKYRVEYEPLVVCSSCLDNTEKNSLKQNLIHLGGHMLNNWTEKCTHLVMTSIKVTIKTICALICCKPIIKPDYFRELIN---AIQQKRPLPEYRSFIPPIDEPSLNPESLDLSENVKRKSIFKDKVFLFLNTKQYMKLSPAVLLGGGKAHLLESLTDTSVLENSTTCVIDVAMTETQLSETQSTQPWISSTLDLLHSKGLRAIPEAEIGLAVINVSTEIYCNPRR 323

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Xenopus
Match: adcy9 (adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-999257])

HSP 1 Score: 123.635 bits (309), Expect = 6.958e-29
Identity = 100/319 (31.35%), Postives = 162/319 (50.78%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWEC---------DLNYITIDDCSKFGTFINKA---PLINKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDH--YLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAML---LTDSDCRDQSLR--VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYR 975
            Y L    Y +GR +  +++    SISR HA L              ++ +TI D SK+GT ++     P + +  ++K  D V FG       +  + V ++  +VCS+ L N ++  LKQ L  +GG++L N  +K  ++VM+++K T K +CALI    I+K D+ + +++    I Q  P P++  ++P I  P      LD   N KRK +F +K F FL  ++   LS AVL GGG A L   LTD+   + S    + +A  ++ + +   T   ++    +L     R I ++EIG AV+  S + YCNP R
Sbjct:   15 YILTGTDYVVGRKNCAILIPDDQSISRCHATLSVSYPSASLSQTNAVSVLTIKDSSKYGTTVSGERMQPAVPR--SLKPSDEVIFGC-----FHSKYRVEYEPLVVCSSCLDNTEKNSLKQNLIHLGGHMLNNWTEKCTHLVMTSIKVTIKTICALICCKPIIKPDYFRELIN---AIQQKRPLPEYRSFIPPIDEPSLNPESLDLSENVKRKSIFKDKVFLFLNTKQYMKLSPAVLLGGGKAHLLESLTDTSVLENSTTCVIDVAMTETQLSETQSTQPWISSTLDLLHSKGLRAIPEAEIGLAVINVSTEIYCNPRR 323

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Xenopus
Match: adcy9 (adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-999257])

HSP 1 Score: 123.635 bits (309), Expect = 7.402e-29
Identity = 100/319 (31.35%), Postives = 162/319 (50.78%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWEC---------DLNYITIDDCSKFGTFINKA---PLINKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDH--YLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAML---LTDSDCRDQSLR--VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYR 975
            Y L    Y +GR +  +++    SISR HA L              ++ +TI D SK+GT ++     P + +  ++K  D V FG       +  + V ++  +VCS+ L N ++  LKQ L  +GG++L N  +K  ++VM+++K T K +CALI    I+K D+ + +++    I Q  P P++  ++P I  P      LD   N KRK +F +K F FL  ++   LS AVL GGG A L   LTD+   + S    + +A  ++ + +   T   ++    +L     R I ++EIG AV+  S + YCNP R
Sbjct:   15 YILTGTDYVVGRKNCAILIPDDQSISRCHATLSVSYPSASLSQTNAVSVLTIKDSSKYGTTVSGERMQPAVPR--SLKPSDEVIFGC-----FHSKYRVEYEPLVVCSSCLDNTEKNSLKQNLIHLGGHMLNNWTEKCTHLVMTSIKVTIKTICALICCKPIIKPDYFRELIN---AIQQKRPLPEYRSFIPPIDEPSLNPESLDLSENVKRKSIFKDKVFLFLNTKQYMKLSPAVLLGGGKAHLLESLTDTSVLENSTTCVIDVAMTETQLSETQSTQPWISSTLDLLHSKGLRAIPEAEIGLAVINVSTEIYCNPRR 323

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Xenopus
Match: adcy9 (adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-999257])

HSP 1 Score: 123.635 bits (309), Expect = 7.637e-29
Identity = 100/319 (31.35%), Postives = 162/319 (50.78%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWEC---------DLNYITIDDCSKFGTFINKA---PLINKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDH--YLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAML---LTDSDCRDQSLR--VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYR 975
            Y L    Y +GR +  +++    SISR HA L              ++ +TI D SK+GT ++     P + +  ++K  D V FG       +  + V ++  +VCS+ L N ++  LKQ L  +GG++L N  +K  ++VM+++K T K +CALI    I+K D+ + +++    I Q  P P++  ++P I  P      LD   N KRK +F +K F FL  ++   LS AVL GGG A L   LTD+   + S    + +A  ++ + +   T   ++    +L     R I ++EIG AV+  S + YCNP R
Sbjct:   29 YILTGTDYVVGRKNCAILIPDDQSISRCHATLSVSYPSASLSQTNAVSVLTIKDSSKYGTTVSGERMQPAVPR--SLKPSDEVIFGC-----FHSKYRVEYEPLVVCSSCLDNTEKNSLKQNLIHLGGHMLNNWTEKCTHLVMTSIKVTIKTICALICCKPIIKPDYFRELIN---AIQQKRPLPEYRSFIPPIDEPSLNPESLDLSENVKRKSIFKDKVFLFLNTKQYMKLSPAVLLGGGKAHLLESLTDTSVLENSTTCVIDVAMTETQLSETQSTQPWISSTLDLLHSKGLRAIPEAEIGLAVINVSTEIYCNPRR 337

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Mouse
Match: Nbn (nibrin [Source:MGI Symbol;Acc:MGI:1351625])

HSP 1 Score: 122.865 bits (307), Expect = 9.157e-29
Identity = 97/316 (30.70%), Postives = 152/316 (48.10%), Query Frame = 1

Query:   94 FLPSVTYTIGRVSADVILTH-TSISRKHAVL--------IWECD-LNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLV------NEISLILKGINK---RLINDSEIGFAVMLASVDEYCNP 969
             L  V Y +GR +  +++ +  SISR HAVL        + + D +  +TI D SK+GTF+N+  +       +K GD VTFG          F V ++  +VCS+ L  + + +L Q +  +GG    N  ++  ++VMS +K T K +CALI    I+K ++    +   +   Q  PD + + P I  P      +D     +RK +F  K F FL  ++   LS AV  GGG A L+ + D  +QS       C  +V      T L+        I LI+  + +   R I ++EIG AV+  + + YCNP
Sbjct:   18 LLAGVEYVVGRKNCGILIENDQSISRNHAVLTVNFPVTSLSQTDEIPTLTIKDNSKYGTFVNEEKMQTGLSCTLKTGDRVTFGV-----FESKFRVEYEPLVVCSSCLDVSGKTVLNQAILQLGGLTANNWTEECTHLVMSAVKVTIKTICALICGRPIIKPEYFSEFLKAVESKKQP-PDIESFYPPIDEPAIGSKSVDLSGRHERKQIFKGKTFVFLNAKQHKKLSSAVAFGGGEARLMAEDDEEEQSFFSAPGTC--VVDVGITNTQLIISHSQKKWIHLIMDTLQRNGLRPIPEAEIGLAVIFMTTENYCNP 325

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Mouse
Match: Nbn (nibrin [Source:MGI Symbol;Acc:MGI:1351625])

HSP 1 Score: 121.709 bits (304), Expect = 1.066e-28
Identity = 98/317 (30.91%), Postives = 154/317 (48.58%), Query Frame = 1

Query:   94 FLPSVTYTIGRVSADVILTH-TSISRKHAVL--------IWECD-LNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVT-TNLV------NEISLILKGINK---RLINDSEIGFAVMLASVDEYCNP 969
             L  V Y +GR +  +++ +  SISR HAVL        + + D +  +TI D SK+GTF+N+  +       +K GD VTFG          F V ++  +VCS+ L  + + +L Q +  +GG    N  ++  ++VMS +K T K +CALI    I+K ++    +   +   Q  PD + + P I  P      +D     +RK +F  K F FL  ++   LS AV  GGG A L+ + D  +QS       C   V+   +T T L+        I LI+  + +   R I ++EIG AV+  + + YCNP
Sbjct:   18 LLAGVEYVVGRKNCGILIENDQSISRNHAVLTVNFPVTSLSQTDEIPTLTIKDNSKYGTFVNEEKMQTGLSCTLKTGDRVTFGV-----FESKFRVEYEPLVVCSSCLDVSGKTVLNQAILQLGGLTANNWTEECTHLVMSAVKVTIKTICALICGRPIIKPEYFSEFLKAVESKKQP-PDIESFYPPIDEPAIGSKSVDLSGRHERKQIFKGKTFVFLNAKQHKKLSSAVAFGGGEARLMAEDDEEEQSFFSAPGTC---VVDVGITNTQLIISHSQKKWIHLIMDTLQRNGLRPIPEAEIGLAVIFMTTENYCNP 325

[Close]

BLAST of FHA domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9DE07|NBN_CHICK (Nibrin OS=Gallus gallus OX=9031 GN=NBN PE=1 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.501e-33
Identity = 97/315 (30.79%), Postives = 155/315 (49.21%), Query Frame = 1

Query:   94 FLPSVTYTIGRVS-ADVILTHTSISRKHAVLI---------WECDLNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHC-------DSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYR 975
             L    Y +GR + A +I    SISR HAVL              +  +TI D SK+GTF+N + L     +++ GD V FG          F V ++  +VCS+ L  A +  L + +  +GG ++     +  +++M ++K T K +CALI    IVK +F   ++   +   Q LP P+ + P +  P      +D   +P+RK +F+ K F FLT ++   L  AV+ GGG A L+ + + ++ SL V    C       +S ++      N  + I  +L+  N R I ++EIG AV+  S + YCNP R
Sbjct:   16 LLVGTEYVVGRKNCAFLIQDDQSISRSHAVLTVSRPETTHSQSVSVPVLTIKDTSKYGTFVNGSKLSGASRSLQSGDRVNFGV-----FESKFRVEYESLVVCSSCLDVAQKTALNEAIQQLGGLVVNEWTKECTHLIMESVKVTVKTICALICGRPIVKPEFFSELMKAVQSRQQ-LPTPESFYPSVDEPAIGIDNMDLSGHPERKKIFSGKTFVFLTAKQHKKLGPAVILGGGEAKLMAE-ERKETSLLVSPEVCVVDVGVTNSQILGSESMRNWTDSILAVLESNNLRAIPEAEIGLAVIFMSTEIYCNPQR 323

[Close]

BLAST of FHA domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5I2W8|NBN_DANRE (Nibrin OS=Danio rerio OX=7955 GN=nbn PE=2 SV=2)

HSP 1 Score: 137.502 bits (345), Expect = 1.268e-32
Identity = 103/337 (30.56%), Postives = 161/337 (47.77%), Query Frame = 1

Query:   55 WKF---ENKNKNIVKYFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDP--DHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTN-----------LVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKEN 1005
            WK    E+  ++++   L    Y +GR + +++LT+  SISR HAVL        +T+ D SK+GTF+N   L       ++ G  +TFG  Q    L    +   +VCS+ + N  +V L Q +  +GG L+ +      ++VM  +K T K +CAL+    IVK  F  A+    K + Q LP P  + + P+I  P      +D    P+RK LF  K F FL+ +++  LS AV  GGG++ LL +      +L V L    S  + + ++ N            ++ +  IL     R I +SE+G A +  S   YCNP  +L     K N
Sbjct:    2 WKLQPTESGGESVI--LLAGQEYVVGRKNCEILLTNDQSISRVHAVLT--VTEQAVTLKDSSKYGTFVNGEKLESGSTKTLQTGYKITFGVFQSKFSLEKECI---VVCSSCVDNEGKVTLSQDIRSVGGRLVSSWTSDCTHLVMPTVKVTIKTICALLCCRPIVKPAFFSALS---KAVQQKLPLPKAERFRPQIDEPSLARDDVDLSARPERKSLFKGKTFLFLSSKQMKRLSVAVSCGGGVSQLLDEG-----ALPVSLLESSSTCVLDMISGNSQPVISPASKKWLDSVGQILHRKGLRFITESEVGLAAIHVSNQTYCNPCSSLQSESVKTN 323

[Close]

BLAST of FHA domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9R207|NBN_MOUSE (Nibrin OS=Mus musculus OX=10090 GN=Nbn PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 6.405e-28
Identity = 97/316 (30.70%), Postives = 152/316 (48.10%), Query Frame = 1

Query:   94 FLPSVTYTIGRVSADVILTH-TSISRKHAVL--------IWECD-LNYITIDDCSKFGTFINKAPL-INKKGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLV------NEISLILKGINK---RLINDSEIGFAVMLASVDEYCNP 969
             L  V Y +GR +  +++ +  SISR HAVL        + + D +  +TI D SK+GTF+N+  +       +K GD VTFG          F V ++  +VCS+ L  + + +L Q +  +GG    N  ++  ++VMS +K T K +CALI    I+K ++    +   +   Q  PD + + P I  P      +D     +RK +F  K F FL  ++   LS AV  GGG A L+ + D  +QS       C  +V      T L+        I LI+  + +   R I ++EIG AV+  + + YCNP
Sbjct:   18 LLAGVEYVVGRKNCGILIENDQSISRNHAVLTVNFPVTSLSQTDEIPTLTIKDNSKYGTFVNEEKMQTGLSCTLKTGDRVTFGV-----FESKFRVEYEPLVVCSSCLDVSGKTVLNQAILQLGGLTANNWTEECTHLVMSAVKVTIKTICALICGRPIIKPEYFSEFLKAVESKKQP-PDIESFYPPIDEPAIGSKSVDLSGRHERKQIFKGKTFVFLNAKQHKKLSSAVAFGGGEARLMAEDDEEEQSFFSAPGTC--VVDVGITNTQLIISHSQKKWIHLIMDTLQRNGLRPIPEAEIGLAVIFMTTENYCNP 325

[Close]

BLAST of FHA domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9JIL9|NBN_RAT (Nibrin OS=Rattus norvegicus OX=10116 GN=Nbn PE=1 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 1.334e-26
Identity = 95/314 (30.25%), Postives = 152/314 (48.41%), Query Frame = 1

Query:   94 FLPSVTYTIGRVSADVILTH-TSISRKHAVL--------IWECD-LNYITIDDCSKFGTFINKAPLINK-KGNIKDGDIVTFGAQPGVDLNCSF-VAFD--IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIV----MQEAVTTNLVNEISLILKGINK---RLINDSEIGFAVMLASVDEYCNP 969
             L  V Y +GR +  +++ +  SISR HAVL        + + D +  +TI D SK+GTFIN+  + N     +K GD VTFG          F V ++  +VCS+ L  + + +L Q +  +GG    +  ++  ++ MS++K T K +CALI    IVK ++    +   +   Q  P+ + + P I  P      +D     +RK +F  K F FL  ++   L  AV+ GGG A L+ +    +QS       C   V     Q  +T +    I LI+  + +   R I ++EIG AV+  + + YCNP
Sbjct:   18 LLAGVEYIVGRKNCAILIENDQSISRNHAVLRVNFPVTSLSQTDEIPTLTIKDNSKYGTFINEEKMQNGLSSTLKTGDRVTFGV-----FESKFRVEYEPLVVCSSCLDVSGKTVLNQAILQLGGLTANSWTEECTHLAMSSVKVTIKTICALICGRPIVKPEYFSEFLKAVESKTQP-PEIESFYPPIDEPAIGNKSVDLSGRRERKQIFKGKTFVFLNAKQHKKLGSAVVFGGGEARLMAEGGEEEQSFFSAPGTCVVDVGITNTQLIITDSQRKWIHLIMDILQRHGLRPIPEAEIGLAVIFMTTESYCNP 325

[Close]

BLAST of FHA domain-containing protein vs. UniProt/SwissProt
Match: sp|O60934|NBN_HUMAN (Nibrin OS=Homo sapiens OX=9606 GN=NBN PE=1 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 6.411e-24
Identity = 87/317 (27.44%), Postives = 143/317 (45.11%), Query Frame = 1

Query:   94 FLPSVTYTIGRVSADVILTH-TSISRKHAVLIWEC---------DLNYITIDDCSKFGTFINKAPLINK-KGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLVNEISLI--------------LKGINKRLINDSEIGFAVMLASVDEYCNP 969
             L  V Y +GR +  +++ +  SISR HAVL             ++  +T+ D SK+GTF+N+  + N     +K GD +TFG   G      +       S L  + +  L Q +  +GG  + N  ++  ++VM ++K T K +CALI    IVK ++    +   +   Q  P  + + P +  P      +D     +RK +F  K F FL  ++   LS AV+ GGG A L+T+ +  + +  +    C        V T + N  +LI              L+    R I ++EIG AV+  +   YC+P
Sbjct:   18 LLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKDNSKYGTFVNEEKMQNGFSRTLKSGDGITFGVF-GSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQP-PQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTC-------VVDTGITNSQTLIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDP 325

[Close]

BLAST of FHA domain-containing protein vs. TrEMBL
Match: A0A4S2MI43 (FHA domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000762 PE=4 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 7.494e-40
Identity = 102/316 (32.28%), Postives = 164/316 (51.90%), Query Frame = 1

Query:  112 YTIGRVSADVILTHTSISRKHAVLIWEC----DLNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLD---YKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSI--------------VMQEAVTTNLVNEISLI---LKGINKRLINDSEIGFAVMLASVDEYCNPYRALPK 987
            +T+GR+ AD+I+   S+SRKHA L  +      L +I + D SKFGTF+N  PL   K  +K GD VTFG QPGV+ +      ++  S+++ A+R  +K   +++ G L+  C D   ++VM +L  T KVVCAL+  + IV   +++  ++    I  + P+ +++LP +   ++  +  D   + PN KR  LF  K F+ LT    S LS  V  GGG  +L+  S+   Q    +   C ++              V+       L +   ++   L+GI +R I +SE+GFAV+  S + YCNP    P+
Sbjct:   26 FTVGRIDADIIIPDKSVSRKHAGLEIQAAQSGGLEFI-LHDHSKFGTFVNGIPLNGSKRILKKGDKVTFGVQPGVNFSLQETVLNVAVSSMNKAERQRIKTITNLLHGELVPTCTDSCTFLVMPHLVVTVKVVCALLAMVPIVTPLYMEKFLEAASYIPFVRPNFENFLPPVR--EERLVDSDAIRFLPNAKRPSLFMGKVFFVLTHAHFSELSPIVRLGGGKVVLIDSSEAFVQHYEEQSGTCGNVERDLQEVLRREGSCVIHAQSVPGLEDWQRVVYSALRGIRRRPILESELGFAVLYISTNGYCNPETKCPE 338

[Close]

BLAST of FHA domain-containing protein vs. TrEMBL
Match: A0A4V6AQV8 (Nibrin Nijmegen breakage syndrome protein 1-like protein OS=Collichthys lucidus OX=240159 GN=D9C73_016161 PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 4.181e-35
Identity = 112/323 (34.67%), Postives = 155/323 (47.99%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVIL-THTSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLI-NKKGNIKDGDIVTFGAQPGVDLNCSFVAF---DIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDP--DHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDC-RD-----QSLRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKENNNPK 1017
            Y L S  Y +GR + D++L +  SISR HA L        +T+ D SK+GTF+N   L  N   N+K GD VTFG          F       +VCS+ L N  +  L Q L  +GG L+        ++VM   K T K +CAL+    IVK +F     +  K   Q LP P  + ++PEI  P      ++    P RK LF  K F FL+ ++L  LS AV  GGG + LL +    RD     QS  +      S  +  + TT   N +  I++    R+I +SEIG A + AS D+YCNP R +  S      NP+
Sbjct:   15 YLLSSKDYVVGRKNCDILLPSDQSISRAHAHLT--ATGQTLTLKDTSKYGTFVNGQQLTANTPANLKSGDNVTFGV-----FESKFSVEHQKPVVCSSCLDNDGKTSLTQALLAVGGKLVNTWTQDCTHLVMPTAKVTIKTICALLCCRPIVKPEF---FSELGKAAQQKLPPPKAESFIPEIDEPSLSKEDVNLGAIPVRKQLFAGKTFIFLSAKQLKRLSAAVGFGGGRSQLLEEGSLPRDVLESPQSCVIDATTGSSQPLLSSSTTEWANTVKNIVQRKGLRVIAESEIGLAAIYASCDQYCNPSRLITDSESLPKVNPR 327

[Close]

BLAST of FHA domain-containing protein vs. TrEMBL
Match: A0A1S3K6I0 (nibrin OS=Lingula unguis OX=7574 GN=LOC106179244 PE=4 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 6.986e-35
Identity = 102/308 (33.12%), Postives = 164/308 (53.25%), Query Frame = 1

Query:  118 IGRVSADVILTH-TSISRKHAVLIWECDLNYI---------TIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIH----NPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDS--DCRDQSLRVK---LAHCDSIVMQEAVTTN---LVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYR 975
            IGR   D+++    S+SRKHAV I     N++         TI D SKFGTF+N +  I  +  +KDGD + FGAQ    +      F +  S L N+ +  +K  +  +GG++L +   + + +VMS +  T KVVCALI Q  IV  +++  +++ +   N+ LP+PD ++P +     NPD+    + +K NP R+ LF+  +F FL+ ++   LS AV   GG  +L+ +   D  D+ L  +   + +C+     + ++ N    V  +   LK   +R I D+E+GFAV+  S D YCNP R
Sbjct:   23 IGRKDCDILIEGDQSMSRKHAVAIVVHPENHLPFPHKPPVLTIKDTSKFGTFVNGS-RIQGERELKDGDEIKFGAQSSFRVKRE--PFIVTTSCLDNSVKKSVKGVIRQLGGHVLGDWRRECDMLVMSTISVTIKVVCALISQKPIVTPEYLTQLLEAYDQ-NKQLPNPDDFIPALAELLLNPDE----VSFKKNPARQTLFSGMKFIFLSPKQFRKLSIAVELAGGTPVLMEEGTDDNNDKVLIERGTCVMYCEPPTNPQLLSKNARDWVTHVQRYLKRHKQRQIQDAELGFAVLYCSTDSYCNPER 322

[Close]

BLAST of FHA domain-containing protein vs. TrEMBL
Match: A0A4W6BPE5 (FHA domain-containing protein OS=Lates calcarifer OX=8187 GN=nbn PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.060e-34
Identity = 110/323 (34.06%), Postives = 158/323 (48.92%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLI-NKKGNIKDGDIVTFGAQPGVDLNCSFVA---FDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILP--DPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDC-RD-----QSLRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKENNNPK 1017
            Y L    Y +GR + D++LT+  SISR HA L        +T+ D SK+GTF+N   L  N   N+K GD VTFGA      N  F       +VCS+ L N  +  L Q L  +GG L+    +   ++ M ++K T K + AL+    IVK +F     +    + Q LP   P+ ++PEI  P      ++ K  P RK LF  K F FL+ ++L  LS AV  G G + LL +    RD     QS  V +    S  +  +  T+  N +  I++    R+I +SEIG A + AS D+YCNP   +P S       P+
Sbjct:   15 YLLARKEYVVGRKNCDILLTNDQSISRAHAHLT--ATDQILTLKDTSKYGTFVNSQRLTDNTPVNLKSGDSVTFGA-----FNSKFSVDHQKPVVCSSCLDNDGKASLSQALLALGGKLVNTWTEDCTHLAMPSVKVTIKTISALLCCRPIVKPEF---FSELHNAVQQKLPPPKPESFIPEIDEPSLSKEDVNLKVIPGRKHLFTEKTFIFLSAKQLKRLSAAVSFGRGKSQLLEEGSLPRDLLESPQSCVVDVMTGSSQPLLSSSATDWANSVKNIVERKGLRVIPESEIGLAAIYASCDKYCNPSSVMPDSESAPKVKPR 327

[Close]

BLAST of FHA domain-containing protein vs. TrEMBL
Match: A0A4W6BIH3 (FHA domain-containing protein OS=Lates calcarifer OX=8187 GN=nbn PE=4 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 5.644e-34
Identity = 110/323 (34.06%), Postives = 158/323 (48.92%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLI-NKKGNIKDGDIVTFGAQPGVDLNCSFVA---FDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILP--DPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDC-RD-----QSLRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKENNNPK 1017
            Y L    Y +GR + D++LT+  SISR HA L        +T+ D SK+GTF+N   L  N   N+K GD VTFGA      N  F       +VCS+ L N  +  L Q L  +GG L+    +   ++ M ++K T K + AL+    IVK +F     +    + Q LP   P+ ++PEI  P      ++ K  P RK LF  K F FL+ ++L  LS AV  G G + LL +    RD     QS  V +    S  +  +  T+  N +  I++    R+I +SEIG A + AS D+YCNP   +P S       P+
Sbjct:   15 YLLARKEYVVGRKNCDILLTNDQSISRAHAHLT--ATDQILTLKDTSKYGTFVNSQRLTDNTPVNLKSGDSVTFGA-----FNSKFSVDHQKPVVCSSCLDNDGKASLSQALLALGGKLVNTWTEDCTHLAMPSVKVTIKTISALLCCRPIVKPEF---FSELHNAVQQKLPPPKPESFIPEIDEPSLSKEDVNLKVIPGRKHLFTEKTFIFLSAKQLKRLSAAVSFGRGKSQLLEEGSLPRDLLESPQSCVVDVMTGSSQPLLSSSATDWANSVKNIVERKGLRVIPESEIGLAAIYASCDKYCNPSSVMPDSESAPKVKPR 327

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Cavefish
Match: nbn (nibrin [Source:NCBI gene;Acc:103021427])

HSP 1 Score: 147.517 bits (371), Expect = 7.017e-37
Identity = 95/303 (31.35%), Postives = 149/303 (49.17%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLI-NKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLR------VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNP 969
            Y LP   Y +GR + D++L +  SISR HA L        +T+ D SK+GTF+N   L  N    + DGD +TFG  Q    L  + +   +VCS+ + N  +  L + +  +GG L+ + +    ++VM  +K T K +CAL+    IVK +F   +    +   ++LP   ++ PEI  P      +D  P P+RK LF +K F FL  +++  LS+A+  GGG+  LL +       L+      + +    S  +    T   V+ +  +L     R I +SEIG A +  S   YCNP
Sbjct:   15 YLLPGKEYVVGRKNCDILLANDQSISRVHAQLT--VADQTVTLKDSSKYGTFVNDEQLTTNSTKTVHDGDRLTFGVFQSKFRLKLNSM---VVCSSCVDNEGKAALSKAVQSVGGRLVNSWSPDCTHLVMPTVKVTIKTICALLCCRPIVKAEFFTELSRAVQRKEKVLPAAQNFKPEIDEPSLTKQDVDLGPRPERKSLFRDKTFVFLNVKQMKRLSQAITCGGGVTQLLEEGSLPLPLLQSTGTCVIDVGAGASQALVPPATKKWVDSVGQVLHRNGLRFIAESEIGLAAIYVSNQTYCNP 312

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Cavefish
Match: nbn (nibrin [Source:NCBI gene;Acc:103021427])

HSP 1 Score: 147.517 bits (371), Expect = 7.928e-37
Identity = 95/303 (31.35%), Postives = 149/303 (49.17%), Query Frame = 1

Query:   91 YFLPSVTYTIGRVSADVILTH-TSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLI-NKKGNIKDGDIVTFGA-QPGVDLNCSFVAFDIVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLR------VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNP 969
            Y LP   Y +GR + D++L +  SISR HA L        +T+ D SK+GTF+N   L  N    + DGD +TFG  Q    L  + +   +VCS+ + N  +  L + +  +GG L+ + +    ++VM  +K T K +CAL+    IVK +F   +    +   ++LP   ++ PEI  P      +D  P P+RK LF +K F FL  +++  LS+A+  GGG+  LL +       L+      + +    S  +    T   V+ +  +L     R I +SEIG A +  S   YCNP
Sbjct:   15 YLLPGKEYVVGRKNCDILLANDQSISRVHAQLT--VADQTVTLKDSSKYGTFVNDEQLTTNSTKTVHDGDRLTFGVFQSKFRLKLNSM---VVCSSCVDNEGKAALSKAVQSVGGRLVNSWSPDCTHLVMPTVKVTIKTICALLCCRPIVKAEFFTELSRAVQRKEKVLPAAQNFKPEIDEPSLTKQDVDLGPRPERKSLFRDKTFVFLNVKQMKRLSQAITCGGGVTQLLEEGSLPLPLLQSTGTCVIDVGAGASQALVPPATKKWVDSVGQVLHRNGLRFIAESEIGLAAIYVSNQTYCNP 312

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004190.1 (pep scaffold:Pmarinus_7.0:GL476745:100200:113784:1 gene:ENSPMAG00000003821.1 transcript:ENSPMAT00000004190.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 110.923 bits (276), Expect = 1.227e-26
Identity = 92/319 (28.84%), Postives = 149/319 (46.71%), Query Frame = 1

Query:  112 YTIGRVSADVILTH-TSISRKHAVLI---WECDLN------YITIDDCSKFGTFINKAPLIN-KKGNIKDGDIVTFGAQPGVDLNCSFVAFD--IVCSTLSNAD-RVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDH--YLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNL------------VNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALP 984
            + +GR    +++ +  SISR HAVL     E  L        +++ D SK+GTF+N   L +  +  +  GD++ FG    V  +   VA+   +VCS+  +A+ + +L   L  +GG+ +        ++VM+++K T K +CALI  I IV+  F   +V   +  +   P P H  + P    P      LD  P P+R+ LF  K F FL  ++   L+  +   GG A+ L     R +  R   A C  +V  EA  + L              ++ + L  +  R+I ++E+G AV+  S   YCNP   LP
Sbjct:   29 HVVGRKDCGILIGNDQSISRVHAVLTVSHAESSLARPSCRPALSVKDSSKYGTFVNGEKLTSGSERALCCGDLLKFG----VFESKYRVAYSPLVVCSSCLDAESKHVLSSALLALGGHFVSQWTSDCTHLVMTSIKVTVKTICALITSIPIVQPVFFSTLVTAIRSRS---PQPSHSSFWPLADEPGINPGDLDLTPRPERRLLFQGKTFQFLHAKQHKRLAVPIALAGGAALCLCSPGQRAEP-RHGEAFC--VVEPEAGCSQLSSQVLAQDARAWAAQVGVRLHTLGLRMITEAEVGLAVVYCSTHTYCNPSARLP 337

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Nematostella
Match: EDO41630 (Nibrin [Source:UniProtKB/TrEMBL;Acc:A7S415])

HSP 1 Score: 120.168 bits (300), Expect = 2.443e-28
Identity = 98/330 (29.70%), Postives = 154/330 (46.67%), Query Frame = 1

Query:   58 KFENKNKNIVKYFLPSVTYTIGRVSADVIL-THTSISRKHAVLIW---------ECDLNYITIDDCSKFGTFINKAPLINKKG-NIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLL-ENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEA--------VTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNP-YRALP 984
            K ++ N+  V+ F+    Y +GR    +++    SISRKHAV               L  +T+ D SK+G  +N   L +     + +GD++ FG         S++      S L    + +LK+ +  +GG+LL E   D   ++VM  L  T KV+ AL     IV  +FV  M+   +  N+ L D  +YLP I        G  + PNP RK LF+ K F FL++++     EAV   GG   L   +DC   S  +  ++C  ++M +A         T   + +   +LK    R I +SE+G A++  S+  YCNP   A+P
Sbjct:   10 KAQDINQGTVR-FIVDKEYVVGRKDCQILINGDPSISRKHAVFTIGFNISDLGDTSKLPEVTVRDTSKYGVSVNGTKLESGGSCQLANGDVILFGTH-NSSYRLSYIQTVFATSCLDTNKKKILKKQIHKLGGHLLSEWRRDCTTHLVMEGLTFTVKVIQALASCCPIVTPEFVDEMIKSSEE-NRPLQDYRNYLPPIKEETIKSDGEKFLPNPDRKSLFSGKNFVFLSKKQYKRTFEAVEQCGGRTSLYESADCLVDSENLVASNC-CVMMVDAKERESLAPATRQFIGQAMDVLKRHRMRPIPESELGLAILQVSLSTYCNPNMEAVP 335

[Close]

BLAST of FHA domain-containing protein vs. Ensembl Medaka
Match: nbn (nibrin [Source:NCBI gene;Acc:101172437])

HSP 1 Score: 116.316 bits (290), Expect = 1.059e-26
Identity = 93/311 (29.90%), Postives = 141/311 (45.34%), Query Frame = 1

Query:   70 KNKNIVKYFLPSVTYTIGRVSADVIL-THTSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFD---IVCST-LSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLR------VKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNP 969
            +N     Y      Y +GR +  ++L +  SISR HA L        +T+ D SK+GTF+     +    N+  GD VTFG       +  F       +VCS+ L N  +  L + L  +GG L+ + +    ++ M  +K T K + AL+    IVK +F   +    +   +  P P+ + PEI  P      ++      RK LF  K F FL  ++L  LS AV  GGG + LL +       L+      V +    S ++  A  T   N +  +L     R+I +SEIG A + AS D+YCNP
Sbjct:    8 ENGGETHYIFCGKEYVVGRKNCAILLPSDQSISRAHANLT--ATDQTLTLKDSSKYGTFVRNQ-RVTSPVNLTPGDEVTFGV-----FHSKFRVIHQKLVVCSSCLDNDGKASLTEALLALGGKLVNSWSLDCTHLAMPTVKVTIKTISALLCCCPIVKPEFFSELNSAVQQ-KRPPPKPESFNPEIDEPVLSDENVNLGAVSARKQLFRGKTFIFLNAKQLKRLSGAVRFGGGSSQLLEEGALPCNLLQSPNTCVVDILTASSQILLPASITEWANSVKNLLARKGLRVIPESEIGLAAIYASCDQYCNP 309

[Close]

BLAST of FHA domain-containing protein vs. Planmine SMEST
Match: SMESG000022990.1 (SMESG000022990.1)

HSP 1 Score: 1141.33 bits (2951), Expect = 0.000e+0
Identity = 577/581 (99.31%), Postives = 577/581 (99.31%), Query Frame = 1

Query:   49 MPWKFENKNKNIVKYFLPSVTYTIGRVSADVILTHTSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKENNNPKQNPFPSLDKLPCKEEPSTNHPNKQVHNNSLGELRAHDINAIDFXXXXXXXXXXXXXXXXXXXNSNAHQHSLRRNQENNSPKPNLSVSKFFNLSTTNLPTMKRKFAMHSHDEFNNDFDNISIPGLQRIPANTLILEPTSRTIFSNFQDEEQNAHNIFPKKRKMNNTTDRSVSSCKIQNMSQTTKLVTDKQLFDLQLIREIPTRIQINPANEMDIRSCMENVKFAQLIKEKYIERKNYKMFIKSSPIYIERNSVPLVQFR 1791
            MPWK ENKNKNIVKYFLPSVTYTIGRVSADVILTHTSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKENNNPKQNPFPSLDKLPCKEEPSTNHPNKQVHNNSLGELRAHDINAIDFDRDRDQIITISDTDSESDTNSNAHQHSLRRNQENNSPKPNLSVSKFFNLSTTNLPTMKRKFAMHSHDEFNNDFDNISIPGLQRIPANTLILE TSRTIFSNFQDEEQNAHNIFPKKRKMNNTTDRSVSSCKIQNMSQTTKLVTDKQLFDLQLIREIPTRIQINPANEMDIRSC ENVKFAQLIKEKYIE KNYKMFIKSSPIYIERNSVPLVQFR
Sbjct:    1 MPWKLENKNKNIVKYFLPSVTYTIGRVSADVILTHTSISRKHAVLIWECDLNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVAFDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVVCALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLDYKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQSLRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLASVDEYCNPYRALPKSHDKENNNPKQNPFPSLDKLPCKEEPSTNHPNKQVHNNSLGELRAHDINAIDFDRDRDQIITISDTDSESDTNSNAHQHSLRRNQENNSPKPNLSVSKFFNLSTTNLPTMKRKFAMHSHDEFNNDFDNISIPGLQRIPANTLILESTSRTIFSNFQDEEQNAHNIFPKKRKMNNTTDRSVSSCKIQNMSQTTKLVTDKQLFDLQLIREIPTRIQINPANEMDIRSCTENVKFAQLIKEKYIECKNYKMFIKSSPIYIERNSVPLVQFR 581

The following BLAST results are available for this feature:

BLAST of FHA domain-containing protein vs. Ensembl Human
BLAST of FHA domain-containing protein vs. Ensembl Celegans
BLAST of FHA domain-containing protein vs. Ensembl Fly
BLAST of FHA domain-containing protein vs. Ensembl Zebrafish
BLAST of FHA domain-containing protein vs. Ensembl Xenopus
BLAST of FHA domain-containing protein vs. Ensembl Mouse
BLAST of FHA domain-containing protein vs. UniProt/SwissProt
BLAST of FHA domain-containing protein vs. TrEMBL
BLAST of FHA domain-containing protein vs. Ensembl Cavefish
BLAST of FHA domain-containing protein vs. Ensembl Sea Lamprey
BLAST of FHA domain-containing protein vs. Ensembl Yeast
BLAST of FHA domain-containing protein vs. Ensembl Nematostella
BLAST of FHA domain-containing protein vs. Ensembl Medaka
BLAST of FHA domain-containing protein vs. Planmine SMEST

BLAST of FHA domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
NBN	1.335e-24	27.44	nibrin [Source:HGNC Symbol;Acc:HGNC:7652]	[more]
NBN	1.510e-16	26.64	nibrin [Source:HGNC Symbol;Acc:HGNC:7652]	[more]
NBN	1.069e-10	27.67	nibrin [Source:HGNC Symbol;Acc:HGNC:7652]	[more]

BLAST of FHA domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of FHA domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
nbs	8.849e-22	25.89	gene:FBgn0261530 transcript:FBtr0306039	[more]
nbs	1.366e-20	25.72	gene:FBgn0261530 transcript:FBtr0306040	[more]

BLAST of FHA domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
nbn	1.286e-33	30.56	nibrin [Source:NCBI gene;Acc:544655]	[more]
nbn	1.371e-33	30.56	nibrin [Source:NCBI gene;Acc:544655]	[more]
nbn	1.680e-33	30.56	nibrin [Source:NCBI gene;Acc:544655]	[more]
nbn	2.005e-10	30.52	nibrin [Source:NCBI gene;Acc:544655]	[more]

BLAST of FHA domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
adcy9	5.387e-29	31.35	adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-99...	[more]
adcy9	6.958e-29	31.35	adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-99...	[more]
adcy9	7.402e-29	31.35	adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-99...	[more]
adcy9	7.637e-29	31.35	adenylate cyclase 9 [Source:Xenbase;Acc:XB-GENE-99...	[more]

BLAST of FHA domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Nbn	9.157e-29	30.70	nibrin [Source:MGI Symbol;Acc:MGI:1351625]	[more]
Nbn	1.066e-28	30.91	nibrin [Source:MGI Symbol;Acc:MGI:1351625]	[more]

BLAST of FHA domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9DE07\|NBN_CHICK	2.501e-33	30.79	Nibrin OS=Gallus gallus OX=9031 GN=NBN PE=1 SV=1	[more]
sp\|Q5I2W8\|NBN_DANRE	1.268e-32	30.56	Nibrin OS=Danio rerio OX=7955 GN=nbn PE=2 SV=2	[more]
sp\|Q9R207\|NBN_MOUSE	6.405e-28	30.70	Nibrin OS=Mus musculus OX=10090 GN=Nbn PE=1 SV=1	[more]
sp\|Q9JIL9\|NBN_RAT	1.334e-26	30.25	Nibrin OS=Rattus norvegicus OX=10116 GN=Nbn PE=1 S...	[more]
sp\|O60934\|NBN_HUMAN	6.411e-24	27.44	Nibrin OS=Homo sapiens OX=9606 GN=NBN PE=1 SV=1	[more]

BLAST of FHA domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4S2MI43	7.494e-40	32.28	FHA domain-containing protein OS=Opisthorchis feli...	[more]
A0A4V6AQV8	4.181e-35	34.67	Nibrin Nijmegen breakage syndrome protein 1-like p...	[more]
A0A1S3K6I0	6.986e-35	33.12	nibrin OS=Lingula unguis OX=7574 GN=LOC106179244 P...	[more]
A0A4W6BPE5	4.060e-34	34.06	FHA domain-containing protein OS=Lates calcarifer ...	[more]
A0A4W6BIH3	5.644e-34	34.06	FHA domain-containing protein OS=Lates calcarifer ...	[more]

BLAST of FHA domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
nbn	7.017e-37	31.35	nibrin [Source:NCBI gene;Acc:103021427]	[more]
nbn	7.928e-37	31.35	nibrin [Source:NCBI gene;Acc:103021427]	[more]

BLAST of FHA domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000004190.1	1.227e-26	28.84	pep scaffold:Pmarinus_7.0:GL476745:100200:113784:1...	[more]

BLAST of FHA domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of FHA domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO41630	2.443e-28	29.70	Nibrin [Source:UniProtKB/TrEMBL;Acc:A7S415]	[more]

BLAST of FHA domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
nbn	1.059e-26	29.90	nibrin [Source:NCBI gene;Acc:101172437]	[more]

BLAST of FHA domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000022990.1	0.000e+0	99.31	SMESG000022990.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30019275 ID=SMED30019275|Name=FHA domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1810bp
AAATATTTAACTTTATATTGTAATTGGCTTTATTAAAATATATTCTAAAT
GCCTTGGAAGTTTGAAAATAAAAATAAAAATATTGTTAAATATTTTCTTC
CATCCGTTACATATACAATTGGCAGAGTATCTGCAGATGTCATTCTTACC
CACACGTCTATCAGCCGAAAACATGCAGTTTTAATTTGGGAATGTGATCT
CAATTATATTACAATTGATGATTGCTCCAAATTTGGCACATTTATTAATA
AAGCACCTTTAATTAACAAGAAAGGAAATATTAAAGATGGCGATATCGTT
ACTTTTGGTGCTCAACCCGGAGTTGATTTAAATTGTTCATTTGTAGCTTT
TGATATCGTCTGTTCGACTCTGTCTAATGCAGACAGAGTTTTGTTAAAAC
AATATTTGTCTATTATCGGTGGGAATTTGTTAGAAAATTGTAATGATAAA
GTTGAATATATGGTTATGAGTAATTTAAAAGCTACATTTAAGGTTGTATG
TGCTTTAATCAAGCAAATAAAAATTGTTAAAATTGATTTTGTCAAGGCAA
TGGTGGATTATTTTAAAGGAATTAATCAAATTTTACCCGATCCCGATCAT
TACTTGCCAGAAATTCACAATCCAGATGATTGCTTTATTGGATTGGATTA
TAAACCGAATCCTAAGCGCAAATGTTTATTCAACAACAAACAGTTTTATT
TTTTAACTCAAGAAAAACTTTCTCTACTCAGTGAAGCTGTATTGTCCGGT
GGGGGAATTGCAATGCTGCTCACCGATTCCGATTGCCGTGATCAATCGCT
GAGAGTAAAACTTGCCCATTGTGATAGTATTGTAATGCAGGAGGCCGTTA
CAACAAATCTCGTGAATGAAATATCTCTCATTCTGAAAGGAATAAACAAG
CGGTTAATTAATGATTCTGAAATCGGATTCGCCGTTATGCTCGCTTCTGT
TGATGAATATTGCAACCCGTACAGAGCCCTTCCCAAATCGCACGACAAAG
AAAACAACAATCCCAAACAAAATCCGTTTCCTTCACTCGACAAATTGCCT
TGCAAAGAGGAGCCTTCTACAAACCATCCAAACAAGCAAGTGCACAATAA
TTCGCTGGGTGAACTTCGTGCGCACGACATAAACGCCATTGATTTTGATC
GCGATCGCGACCAGATCATAACTATCAGTGATACGGATAGTGAAAGCGAT
ACCAATAGCAATGCTCATCAACATTCACTCAGAAGGAATCAGGAAAACAA
TTCTCCCAAACCAAATTTATCGGTTTCGAAATTCTTTAACTTATCCACAA
CTAATTTGCCGACAATGAAACGGAAATTTGCAATGCATAGTCATGACGAA
TTTAATAATGATTTTGACAATATTTCCATTCCTGGACTGCAAAGGATTCC
AGCTAATACACTGATCCTAGAACCTACCAGTAGAACCATTTTCAGCAACT
TCCAAGATGAAGAACAAAATGCTCATAATATATTTCCAAAGAAAAGAAAA
ATGAACAATACTACTGACAGATCTGTAAGCAGTTGCAAAATACAAAATAT
GTCTCAAACGACAAAATTAGTAACTGATAAACAGTTATTCGACCTACAGC
TGATCAGAGAGATTCCAACGAGAATTCAGATAAATCCCGCCAATGAAATG
GATATTAGATCTTGCATGGAAAATGTCAAATTTGCCCAACTGATCAAAGA
AAAATATATTGAACGTAAAAATTACAAAATGTTTATAAAGAGTTCTCCAA
TTTATATTGAACGTAATTCCGTGCCGCTCGTTCAGTTTCGTTAATAAATA
TTAAACTTTC

protein sequence of SMED30019275-orf-1

>SMED30019275-orf-1 ID=SMED30019275-orf-1|Name=SMED30019275-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=582bp
MPWKFENKNKNIVKYFLPSVTYTIGRVSADVILTHTSISRKHAVLIWECD
LNYITIDDCSKFGTFINKAPLINKKGNIKDGDIVTFGAQPGVDLNCSFVA
FDIVCSTLSNADRVLLKQYLSIIGGNLLENCNDKVEYMVMSNLKATFKVV
CALIKQIKIVKIDFVKAMVDYFKGINQILPDPDHYLPEIHNPDDCFIGLD
YKPNPKRKCLFNNKQFYFLTQEKLSLLSEAVLSGGGIAMLLTDSDCRDQS
LRVKLAHCDSIVMQEAVTTNLVNEISLILKGINKRLINDSEIGFAVMLAS
VDEYCNPYRALPKSHDKENNNPKQNPFPSLDKLPCKEEPSTNHPNKQVHN
NSLGELRAHDINAIDFDRDRDQIITISDTDSESDTNSNAHQHSLRRNQEN
NSPKPNLSVSKFFNLSTTNLPTMKRKFAMHSHDEFNNDFDNISIPGLQRI
PANTLILEPTSRTIFSNFQDEEQNAHNIFPKKRKMNNTTDRSVSSCKIQN
MSQTTKLVTDKQLFDLQLIREIPTRIQINPANEMDIRSCMENVKFAQLIK
EKYIERKNYKMFIKSSPIYIERNSVPLVQFR*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0006302	double-strand break repair
GO:0007095	mitotic G2 DNA damage checkpoint
GO:0000723	telomere maintenance
GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0007049	cell cycle
GO:0007093	mitotic cell cycle checkpoint
GO:0051321	meiotic cell cycle

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000014	zeta neoblast
PLANA:0000240	body wall musculature
PLANA:0000420	parapharyngeal region
PLANA:0000429	neoblast
PLANA:0002075	pigment cell
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell
PLANA:0002142	parenchyma

Vocabulary: **INTERPRO**
Term	Definition
IPR043014	Nibrin_BRCT2_sf
IPR036420	BRCT_dom_sf
IPR040227	Nibrin-rel
IPR000253	FHA_dom
IPR001357	BRCT_dom
IPR032429	Nibrin_BRCT2
IPR008984	SMAD_FHA_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **cellular component**
Term	Definition
GO:0000781	chromosome, telomeric region
GO:0005634	nucleus
GO:0030870	Mre11 complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000253	Forkhead-associated (FHA) domain	SMART	SM00240	FHA_2	coord: 21..71 e-value: 7.0E-9 score: 45.5
IPR000253	Forkhead-associated (FHA) domain	PFAM	PF00498	FHA	coord: 22..87 e-value: 4.7E-14 score: 52.5
IPR000253	Forkhead-associated (FHA) domain	PROSITE	PS50006	FHA_DOMAIN	coord: 22..71 score: 10.291
IPR000253	Forkhead-associated (FHA) domain	CDD	cd00060	FHA	coord: 15..88 e-value: 3.72798E-15 score: 69.3374
None	No IPR available	GENE3D	G3DSA:2.60.200.20		coord: 3..93 e-value: 2.3E-17 score: 64.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 378..408
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 376..408
None	No IPR available	CDD	cd17741	BRCT_nibrin	coord: 103..172 e-value: 2.00816E-11 score: 57.9973
IPR043014	Nibrin, second BRCT domain superfamily	GENE3D	G3DSA:3.40.50.10980		coord: 199..309 e-value: 5.7E-22 score: 80.2
IPR032429	Nibrin, second BRCT domain	PFAM	PF16508	NIBRIN_BRCT_II	coord: 205..307 e-value: 4.7E-13 score: 49.5
IPR036420	BRCT domain superfamily	GENE3D	G3DSA:3.40.50.10190		coord: 96..193 e-value: 4.7E-7 score: 31.8
IPR036420	BRCT domain superfamily	SUPERFAMILY	SSF52113	BRCT domain	coord: 100..171
IPR040227	Nibrin-related	PANTHER	PTHR12162	NIBRIN-RELATED	coord: 13..466
IPR008984	SMAD/FHA domain superfamily	SUPERFAMILY	SSF49879	SMAD/FHA domain	coord: 12..88

Main menu

FHA domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Owlery

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