DUF3421 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019264 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0345371) HSP 1 Score: 98.9821 bits (245), Expect = 1.051e-24 Identity = 54/137 (39.42%), Postives = 76/137 (55.47%), Query Frame = 3 Query: 234 GHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEW-KSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 G +P ++ AG+ ++ RA +NGE +P K+V G+ AY+ + G+EIS ++EVLV H + W S+ GSV +G + EG PLY+GR L GK P H C YIPY QE+R E Y+VL Sbjct: 13 GSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLVGDH------FSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143 HSP 2 Score: 91.2781 bits (225), Expect = 7.367e-22 Identity = 51/145 (35.17%), Postives = 70/145 (48.28%), Query Frame = 3 Query: 210 LSWQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSD-EGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 SW +S G +P ++ G + Y+ R + G PGK+ C YI Y G+E YEVLV Q W ++ G I G+V+GG D +G +Y+GRA LL K P GC Y+PY E K DY++L Sbjct: 76 FSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV-------QPETWIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213 HSP 3 Score: 83.1889 bits (204), Expect = 8.689e-19 Identity = 42/118 (35.59%), Postives = 63/118 (53.39%), Query Frame = 3 Query: 273 YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEW-KSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 Y+ RA + G+ +P K++ + CAY+ Y G E+ +YE+L G Y W + G+V + G + +G PL+IGRA L GK H C YIP+D +E R + Y+VL Sbjct: 173 YVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA------GYGYGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284 HSP 4 Score: 51.9878 bits (123), Expect = 1.023e-7 Identity = 25/66 (37.88%), Postives = 39/66 (59.09%), Query Frame = 3 Query: 216 WQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLV 395 W S+G++P ++V G + +I RA ++G PGKI + +C YI +DG+E+ YEVLV Sbjct: 219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0087792) HSP 1 Score: 98.9821 bits (245), Expect = 1.051e-24 Identity = 54/137 (39.42%), Postives = 76/137 (55.47%), Query Frame = 3 Query: 234 GHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEW-KSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 G +P ++ AG+ ++ RA +NGE +P K+V G+ AY+ + G+EIS ++EVLV H + W S+ GSV +G + EG PLY+GR L GK P H C YIPY QE+R E Y+VL Sbjct: 13 GSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLVGDH------FSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143 HSP 2 Score: 91.2781 bits (225), Expect = 7.367e-22 Identity = 51/145 (35.17%), Postives = 70/145 (48.28%), Query Frame = 3 Query: 210 LSWQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSD-EGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 SW +S G +P ++ G + Y+ R + G PGK+ C YI Y G+E YEVLV Q W ++ G I G+V+GG D +G +Y+GRA LL K P GC Y+PY E K DY++L Sbjct: 76 FSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV-------QPETWIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213 HSP 3 Score: 83.1889 bits (204), Expect = 8.689e-19 Identity = 42/118 (35.59%), Postives = 63/118 (53.39%), Query Frame = 3 Query: 273 YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEW-KSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 Y+ RA + G+ +P K++ + CAY+ Y G E+ +YE+L G Y W + G+V + G + +G PL+IGRA L GK H C YIP+D +E R + Y+VL Sbjct: 173 YVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA------GYGYGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284 HSP 4 Score: 51.9878 bits (123), Expect = 1.023e-7 Identity = 25/66 (37.88%), Postives = 39/66 (59.09%), Query Frame = 3 Query: 216 WQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLV 395 W S+G++P ++V G + +I RA ++G PGKI + +C YI +DG+E+ YEVLV Sbjct: 219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG31086 (gene:FBgn0051086 transcript:FBtr0084989) HSP 1 Score: 93.2041 bits (230), Expect = 7.676e-24 Identity = 55/143 (38.46%), Postives = 73/143 (51.05%), Query Frame = 3 Query: 213 SWQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSV-IGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 SW SNG IP +V AG+ +I RA Y + +P KI+ + AY+ Y +E+ YEVL G YEW + G+V +G + +G LY GR A L GK P HGC YIPYDS+E + Y+VL Sbjct: 5 SWLHFSNGAIPQAAVVAGHDSDGDTIFIGRAFYCNDMLPAKIIPNKGKAYVAYANQEVELENYEVL------SGFNYEWLPAENGEVPPGAVKVGQNVDGETLYAGRGYHAGSLTVGKVHPSHGCLYIPYDSEEVKIFAYEVL 141
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG31086 (gene:FBgn0051086 transcript:FBtr0307341) HSP 1 Score: 93.2041 bits (230), Expect = 7.676e-24 Identity = 55/143 (38.46%), Postives = 73/143 (51.05%), Query Frame = 3 Query: 213 SWQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSV-IGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 SW SNG IP +V AG+ +I RA Y + +P KI+ + AY+ Y +E+ YEVL G YEW + G+V +G + +G LY GR A L GK P HGC YIPYDS+E + Y+VL Sbjct: 5 SWLHFSNGAIPQAAVVAGHDSDGDTIFIGRAFYCNDMLPAKIIPNKGKAYVAYANQEVELENYEVL------SGFNYEWLPAENGEVPPGAVKVGQNVDGETLYAGRGYHAGSLTVGKVHPSHGCLYIPYDSEEVKIFAYEVL 141
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG32633 (gene:FBgn0052633 transcript:FBtr0073800) HSP 1 Score: 93.2041 bits (230), Expect = 1.496e-22 Identity = 54/149 (36.24%), Postives = 77/149 (51.68%), Query Frame = 3 Query: 216 WQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSV-IGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 641 W SNG +P ++V AG+ Y+ RA ++ + +P K++ + AY+ Y +E YEVL G YEW S + G+V +G + +G LY GR A L GK P HGC YIPYDS E + Y+VLC +R + Sbjct: 6 WMHFSNGSVPPNAVVAGHDSDGDTIYVGRAFFSNDMLPAKVIPNKGKAYVAYAREEHELENYEVL------SGYNYEWLSAENGEVPPGAVKVGRNVDGEYLYAGRGYHAGSLTMGKVHPSHGCLYIPYDSDEVKIFAYEVLCQPERWI 148 HSP 2 Score: 73.9442 bits (180), Expect = 2.181e-15 Identity = 45/143 (31.47%), Postives = 71/143 (49.65%), Query Frame = 3 Query: 216 WQKASNGHIPNDSVNAGNS------YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSD-EGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLC 623 W + +IP+ ++ AG+ Y+ R NG+ +P K+V + AY Y E ++ +VL G + W + G++ G + +G PLY+GRA + L GK P HGC YIP+ +E R E+Y+VL Sbjct: 147 WIDTTATNIPDGALVAGHDSNGDTIYVGRVFRNGDLLPAKVVPAKGKAYAAYAQAEHELTDVQVLT------GSGFRWVPASHGNVAPGALSSGPNVDGEPLYVGRAIYCDSLSVGKIHPSHGCIYIPFGGEEVRLENYEVLV 283
BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q66S13|NATT4_THANI (Natterin-4 OS=Thalassophryne nattereri OX=289382 PE=2 SV=1) HSP 1 Score: 51.2174 bits (121), Expect = 2.324e-6 Identity = 44/144 (30.56%), Postives = 64/144 (44.44%), Query Frame = 3 Query: 210 LSWQKASNGHIPNDSVNAGNSYIARAVY-------NGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 L W + NGHIP+++V N+Y+ R Y G Y P K G +C Y Y E + +LV+ NF + EWK G + + +V D LY+ + NK GK H Y+P+ E + +Y VL Sbjct: 76 LEWVRW-NGHIPSNAVKISNTYVGREDYVCRVGCEAGYYTPKK---GPSCFYP-YGFTEQHSKMFHILVNRDNF--EILEWKWKTGGEVPENAVKACRD----LYVAK----NKYGLGKLHQSHHVFYLPWKGTEYKYNEYYVL 204
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8I9L7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020479g1 PE=4 SV=1) HSP 1 Score: 129.028 bits (323), Expect = 5.618e-34 Identity = 66/162 (40.74%), Postives = 98/162 (60.49%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVN-AGNSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFG---GQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKE-DYDVLCFLKR 635 MS KS+ GKG+++ LSW A+NG IP +V+ G+ ++AR +GE +PGK+ G N Y YDG+E C Y+VL +T N G G +EW + G+ K ++I GSD+GRPLY+ +A + + AGK+ P++G Y E+ KE D++VL R Sbjct: 1 MSTFKSSGGKGVDLCLSWVNAANGEIPAGAVSIGGDVFVARCFSDGELLPGKVAAGHNSCYAPYDGEEKCCDTYQVLCETCNHGLTGGPGFEWVAWSGNTAPKHALISGSDQGRPLYVAKANIDGEWCAGKYIPDYGKAYFSCGGAEHEKEDDFEVLVMCTR 162
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H8B2 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012514g1 PE=4 SV=1) HSP 1 Score: 122.094 bits (305), Expect = 1.973e-31 Identity = 65/155 (41.94%), Postives = 96/155 (61.94%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAG-NSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTH-NFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENR-KEDYDVL 620 MS+V +GIE+ LSW ASNG IP +V AG + Y+ARA + GE++PGK+ G AY+ Y +E+ SEYEVLV + G Y+W +G+ + KG+++ G D G PLY+ RA ++ GK QP+ C YI + +E++ +DY+VL Sbjct: 1 MSRVNHNDIEGIELCLSWHPASNGDIPAGAVKAGEDCYVARAKHEGEWVPGKLSAGHTSAYVPYGDEEVPYSEYEVLVSSEIGHFGCGYQWLPAKGNSVPKGALVSGYDNGAPLYVARATFEGEVCCGKAQPDLECAYISWGGKEHKIVDDYEVL 155
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: Q5DHE4 (SJCHGC04453 protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 120.939 bits (302), Expect = 4.991e-31 Identity = 62/151 (41.06%), Postives = 92/151 (60.93%), Query Frame = 3 Query: 189 GKGIEISLSWQKASNGHIPNDSVN-AGNSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFG-GQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKR 635 GKGI+I LSW A++G++P++++N +G+ Y+ R+ ++G +IPGK V G N AY +G+EI EYEVL DT G + YEW+S G + K ++I G PLYI R V ++ GK H C Y P+ E+ DY+VL + K+ Sbjct: 5 GKGIQIPLSWVPATDGNVPHNAINVSGDIYVIRSKHDGHWIPGKAVIGHNTAYYPMNGQEIKTREYEVLCDTSLSGHNKGYEWESESGGNVPKNAIIAGMSGNDPLYIARGSVNGEVCVGKVHYGHNCAYFPWGGNEHSVNDYEVLVWKKK 155
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A267GMN7 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020417g1 PE=4 SV=1) HSP 1 Score: 119.783 bits (299), Expect = 2.050e-30 Identity = 59/151 (39.07%), Postives = 94/151 (62.25%), Query Frame = 3 Query: 183 AAGKGIEISLSWQKASNGHIPNDSVNAGNS-YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTH-NFGGQAYEWKSTRGSVITKGSVIGG-SDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCF 626 A+G +++LSW A G IPN ++ G+ ++ARA + GE IPGK G + AY+ Y G+E + S+YE+L DTH G Y W+ G + +G++IGG S + +P YIGR+++ +++AGK HGC Y P+ QE+R ++++L Sbjct: 14 ASGGNPQVTLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHHAAYVSYGGQEHAVSDYEILCDTHMQCFGCNYTWERCHGRSVPRGALIGGYSADRKPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEILVL 164
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H2V3 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 119.398 bits (298), Expect = 3.627e-30 Identity = 59/151 (39.07%), Postives = 93/151 (61.59%), Query Frame = 3 Query: 183 AAGKGIEISLSWQKASNGHIPNDSVNAGNS-YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTH-NFGGQAYEWKSTRGSVITKGSVIGG-SDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCF 626 A+G +++LSW A G IPN ++ G+ ++ARA + GE IPGK G AY+ Y G+E + S+YE+L DTH G Y W+ G + +G++IGG S + +P YIGR+++ +++AGK HGC Y P+ QE+R ++++L Sbjct: 14 ASGGNPQVTLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHQAAYVSYGGQEHAVSDYEILCDTHMQCFGCNYTWERCHGRSVPRGALIGGYSADRQPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEILVL 164
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000055806.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000027.1:7684441:7697076:-1 gene:ENSAMXG00000043142.1 transcript:ENSAMXT00000055806.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 52.373 bits (124), Expect = 9.767e-8 Identity = 48/146 (32.88%), Postives = 66/146 (45.21%), Query Frame = 3 Query: 204 ISLSWQKASNGHIPNDSVNAGNSYIARAVY-------NGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 +SL W NG +PN +VN N Y R Y G Y P K G C Y Y E S++EVLV+ F + EW+S + SV S +Y+G+ NK GK P H ++P++ +E + YDVL Sbjct: 50 VSLKWV-TWNGSLPNGAVNIYNGYTKRTDYICKYNCEAGFYTPSK---GPYC-YYPYADSEYPASKFEVLVNEDYF--EFLEWRSGSYGSVPPNSVRTCSRGD--IYVGK----NKYGLGKVVPRHEAFFLPWEGKEYWYKKYDVL 182
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000043153.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000027.1:7684441:7688885:-1 gene:ENSAMXG00000043142.1 transcript:ENSAMXT00000043153.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 1.193e-7 Identity = 48/146 (32.88%), Postives = 66/146 (45.21%), Query Frame = 3 Query: 204 ISLSWQKASNGHIPNDSVNAGNSYIARAVY-------NGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 +SL W NG +PN +VN N Y R Y G Y P K G C Y Y E S++EVLV+ F + EW+S + SV S +Y+G+ NK GK P H ++P++ +E + YDVL Sbjct: 87 VSLKWV-TWNGSLPNGAVNIYNGYTKRTDYICKYNCEAGFYTPSK---GPYC-YYPYADSEYPASKFEVLVNEDYF--EFLEWRSGSYGSVPPNSVRTCSRGD--IYVGK----NKYGLGKVVPRHEAFFLPWEGKEYWYKKYDVL 219
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000045654.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000027.1:7684441:7697076:-1 gene:ENSAMXG00000043142.1 transcript:ENSAMXT00000045654.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.9878 bits (123), Expect = 1.420e-7 Identity = 48/146 (32.88%), Postives = 66/146 (45.21%), Query Frame = 3 Query: 204 ISLSWQKASNGHIPNDSVNAGNSYIARAVY-------NGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 +SL W NG +PN +VN N Y R Y G Y P K G C Y Y E S++EVLV+ F + EW+S + SV S +Y+G+ NK GK P H ++P++ +E + YDVL Sbjct: 78 VSLKWV-TWNGSLPNGAVNIYNGYTKRTDYICKYNCEAGFYTPSK---GPYC-YYPYADSEYPASKFEVLVNEDYF--EFLEWRSGSYGSVPPNSVRTCSRGD--IYVGK----NKYGLGKVVPRHEAFFLPWEGKEYWYKKYDVL 210
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000027077.2 (natterin-3-like [Source:NCBI gene;Acc:103027322]) HSP 1 Score: 49.6766 bits (117), Expect = 8.212e-7 Identity = 48/154 (31.17%), Postives = 71/154 (46.10%), Query Frame = 3 Query: 195 GIEISLSWQKASNGHIPNDSVNAGNSYIARAVY-------NGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKS-TRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLK 632 G ++L+W + +G +PN +V N Y R Y G Y P K G C Y + KE E+EVLV+ NF + EWK+ + GSV G G +++G+ NK GK P+ ++P++ E + YDVL K Sbjct: 80 GDNVNLNWT-SWDGSLPNGAVGIYNGYTKRTDYICKYNCEAGFYTPSK---GPYCNY-AWGNKEYHAPEFEVLVNVDNF--EFLEWKAGSYGSVPNYAVRTCG---GIGIFVGK----NKYGLGKVVPQFEAFFLPWEGDEYWYKKYDVLAINK 219
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000034725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000027.1:7691520:7695470:-1 gene:ENSAMXG00000032799.1 transcript:ENSAMXT00000034725.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 49.2914 bits (116), Expect = 8.995e-7 Identity = 47/156 (30.13%), Postives = 64/156 (41.03%), Query Frame = 3 Query: 174 VKSAAGKGIEISLSWQKASNGHIPNDSVNAGNSYIARAVY-------NGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVL 620 V + K ++L W NG +PN +V NSY R Y G Y P K G C Y Y E ++EVLV+ F + EWKS + SV R NK GK P+H ++P++ +E + YDVL Sbjct: 16 VSAPRRKSDNVNLKWV-TWNGLLPNGAVKIYNSYTERTEYICKSDCEAGFYTPSK---GPYC-YYPYGDSEYLALKFEVLVNEDYF--EFLEWKSGSWGSVPPNSV---------------RTCNKYGLGKVHPKHEAFFLPWEGKEYWYKWYDVL 149
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029906.1 (SMESG000029906.1) HSP 1 Score: 322.398 bits (825), Expect = 8.170e-114 Identity = 156/159 (98.11%), Postives = 158/159 (99.37%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAGNSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 641 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAGNSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKS+RGSVI KGSVIGGSDEGRPLYIGRARVA+KLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV Sbjct: 1 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAGNSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSSRGSVIPKGSVIGGSDEGRPLYIGRARVADKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 159
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029910.1 (SMESG000029910.1) HSP 1 Score: 163.696 bits (413), Expect = 3.887e-51 Identity = 74/160 (46.25%), Postives = 111/160 (69.38%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNA-GNSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 641 MSKV + G+ +EIS+SWQ+A +G IP +V A G+ ++ARA +NGEYIPGK+V G + ++CYDGKEI+ S Y+VLVDT YEWK + I ++IGG+D G+PL+I ++ V + + GKF P+HGC ++PY+ E K ++++LC LK+ + Sbjct: 1 MSKVITQYGRAVEISVSWQEAHDGSIPLHAVKADGSCFVARAKHNGEYIPGKLVTGHSHVFVCYDGKEITKSHYQVLVDTSILPTTGYEWKWSEDLEIPPNAIIGGTDNGQPLFIVKSIVEGETVVGKFHPDHGCAHVPYEGLEFEKNEFEILCSLKKHL 160
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074396.1 (SMESG000074396.1) HSP 1 Score: 158.688 bits (400), Expect = 3.107e-49 Identity = 73/161 (45.34%), Postives = 113/161 (70.19%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAG-NSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGG-QAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 641 MSKV S G G+E+SLSW KA+NG IP +V AG +++ARA ++G+YIPGK V G N ++ + G E + + YE+LV++ GG + YEWK T G + KG+++GG ++G PLY+ R+ + ++++ GK+ P+HGC Y+PY +E+R E+ +VLCF + V Sbjct: 1 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 161
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074378.1 (SMESG000074378.1) HSP 1 Score: 158.688 bits (400), Expect = 3.107e-49 Identity = 73/161 (45.34%), Postives = 113/161 (70.19%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAG-NSYIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGG-QAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 641 MSKV S G G+E+SLSW KA+NG IP +V AG +++ARA ++G+YIPGK V G N ++ + G E + + YE+LV++ GG + YEWK T G + KG+++GG ++G PLY+ R+ + ++++ GK+ P+HGC Y+PY +E+R E+ +VLCF + V Sbjct: 1 MSKVSSQYGAGVELSLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTKHV 161
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029913.1 (SMESG000029913.1) HSP 1 Score: 153.68 bits (387), Expect = 2.988e-47 Identity = 72/160 (45.00%), Postives = 110/160 (68.75%), Query Frame = 3 Query: 165 MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAGNS-YIARAVYNGEYIPGKIVRGENCAYICYDGKEISCSEYEVLVDTHNFGGQAYEWKSTRGSVITKGSVIGGSDEGRPLYIGRARVANKLLAGKFQPEHGCTYIPYDSQENRKEDYDVLCFLKRRV 641 MSK+ S G+ +EIS+SW +G+IP+ +V+AG+S Y+ARA + EYIPGK V+G AY+ YDGKEI+ SEY++LVD+ GG + EW TRG+ I S++GG+D RPLYI ++ + +++ GK+ PE C + PY +E+RK ++LC +++ Sbjct: 1 MSKIVSEYGRSVEISVSWIDCQDGNIPDKAVSAGDSCYVARAKHLDEYIPGKFVQGHTSAYVSYDGKEIAKSEYQILVDSSILGGPSIEWHYTRGNQIPYRSIVGGTDNERPLYIAKSAINGEVVVGKYLPEFACGFFPYGGKEHRKSMVEILCCTTKQL 160 The following BLAST results are available for this feature:
BLAST of DUF3421 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of DUF3421 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of DUF3421 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019264 ID=SMED30019264|Name=DUF3421 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=675bpback to top protein sequence of SMED30019264-orf-1 >SMED30019264-orf-1 ID=SMED30019264-orf-1|Name=SMED30019264-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=160bp MSKVKSAAGKGIEISLSWQKASNGHIPNDSVNAGNSYIARAVYNGEYIPGback to top Annotated Terms
The following terms have been associated with this transcript:
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