Protein FAM47E

Overview
NameProtein FAM47E
Smed IDSMED30019223
Length (bp)1400
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein FAM47E (SMED30019223) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein FAM47E (SMED30019223) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein FAM47E (SMED30019223) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein FAM47E (SMED30019223) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30019223

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30019223h1SMcG0018697 dd_Smed_v4_10346_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019223h1SMcG0018697 dd_Smed_v4_10346_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019223h1SMcG0018697 dd_Smed_v4_10346_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019223h1SMcG0018697 dd_Smed_v4_10346_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30019223h1SMcG0018697 dd_Smed_v6_10346_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30019223h1SMcG0018697 dd_Smed_v4_10346_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Protein FAM47E vs. Ensembl Human
Match: FAM47E (family with sequence similarity 47 member E [Source:HGNC Symbol;Acc:HGNC:34343])

HSP 1 Score: 103.605 bits (257), Expect = 1.594e-23
Identity = 101/386 (26.17%), Postives = 168/386 (43.52%), Query Frame = 1 
Query:  187 NKNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGI-IKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNK-------SPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDD---NKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
            +KN    P  L +   +F +   DD R G PP       + ++G       F+     R  +L P  KRQ+  +  K   V SK +P +  R+  +E++E  L  HPLALY +LE ++P E+  ++++++DPD  +      ED  +    + + +K    +  K        P     V+     NA +W+   EEK          K+ ++ EN     + +   S          +I+E  +   F   YETKP+    +H ++L  +P EL+ +V L    +     K+     + K        +   +YGAWYL  K WKK+   E L DP++    ++E  K ++      LA L G   F +FI     R P+FL+ +
Sbjct:   30 HKNGLKFPTSLHSRQLVFPRKGLDDFRKGCPPCTGLVTQVPVEG-------FLPQIYHRAPQLAP-KKRQI--KLLKEADVLSKLSPAQQARKAFLEDVEAHLTPHPLALYLNLEEAMPIELLSKVLEVLDPDRKL------EDTWAYCQDTRKGMKEPTKLLKKHSTQVYLGPSKKTSVS-----NAGQWLY--EEKP--------HKMDLLHENGPRPGLHENGIS----------DIDEEFILKQFDIDYETKPSHDA-LHTMKLNQVPLELKRSVGLSKLQETEFFQKLGYERKLQKPQNPYKPKWVKMRYGAWYLNPKLWKKQRVDEPLVDPEVSHKAQEENFKKELQEQEELLADLHGTVAFKDFILSRGYRTPRFLENM 373
[Close]
BLAST of Protein FAM47E vs. Ensembl Human
Match: FAM47E-STBD1 (FAM47E-STBD1 readthrough [Source:HGNC Symbol;Acc:HGNC:44667])

HSP 1 Score: 87.4261 bits (215), Expect = 3.323e-18
Identity = 90/352 (25.57%), Postives = 153/352 (43.47%), Query Frame = 1 
Query:  187 NKNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGI-IKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNK-------SPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDD---NKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKV 1209
            +KN    P  L +   +F +   DD R G PP       + ++G       F+     R  +L P  KRQ+  +  K   V SK +P +  R+  +E++E  L  HPLALY +LE ++P E+  ++++++DPD  +      ED  +    + + +K    +  K        P     V+     NA +W+   EEK          K+ ++ EN     + +   S          +I+E  +   F   YETKP+    +H ++L  +P EL+ +V L    +     K+     + K        +   +YGAWYL  K WKK+   E L DP++    ++E  K ++
Sbjct:   30 HKNGLKFPTSLHSRQLVFPRKGLDDFRKGCPPCTGLVTQVPVEG-------FLPQIYHRAPQLAP-KKRQI--KLLKEADVLSKLSPAQQARKAFLEDVEAHLTPHPLALYLNLEEAMPIELLSKVLEVLDPDRKL------EDTWAYCQDTRKGMKEPTKLLKKHSTQVYLGPSKKTSVS-----NAGQWLY--EEK--------PHKMDLLHENGPRPGLHENGIS----------DIDEEFILKQFDIDYETKPSHDA-LHTMKLNQVPLELKRSVGLSKLQETEFFQKLGYERKLQKPQNPYKPKWVKMRYGAWYLNPKLWKKQRVDEPLVDPEVSHKAQEENFKKEL 339
[Close]
BLAST of Protein FAM47E vs. Ensembl Human
Match: FAM47E (family with sequence similarity 47 member E [Source:HGNC Symbol;Acc:HGNC:34343])

HSP 1 Score: 80.8777 bits (198), Expect = 2.524e-16
Identity = 69/274 (25.18%), Postives = 120/274 (43.80%), Query Frame = 1 
Query:  541 SEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNK-------SPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAEN-------EHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDD---NKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
            S++  ++++++DPD  +      ED  +    + + +K    +  K        P     V+     NA +W+   EEK          K+ ++ EN       E++ +   +FC WV   G   ++I+E  +   F   YETKP+    +H ++L  +P EL+ +V L    +     K+     + K        +   +YGAWYL  K WKK+   E L DP++    ++E  K ++      LA L G   F +FI     R P+FL+ +
Sbjct:   26 SQLLSKVLEVLDPDRKL------EDTWAYCQDTRKGMKEPTKLLKKHSTQVYLGPSKKTSVS-----NAGQWLY--EEKP--------HKMDLLHENGPRPGLHENVCKAVSDFCKWVTTFG--ISDIDEEFILKQFDIDYETKPSHDA-LHTMKLNQVPLELKRSVGLSKLQETEFFQKLGYERKLQKPQNPYKPKWVKMRYGAWYLNPKLWKKQRVDEPLVDPEVSHKAQEENFKKELQEQEELLADLHGTVAFKDFILSRGYRTPRFLENM 275
[Close]
BLAST of Protein FAM47E vs. Ensembl Human
Match: FAM47A (family with sequence similarity 47 member A [Source:HGNC Symbol;Acc:HGNC:29962])

HSP 1 Score: 55.8398 bits (133), Expect = 1.338e-7
Identity = 42/172 (24.42%), Postives = 87/172 (50.58%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNKNQANHPKF--LLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLI-QHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKI 657
            PWY  +   K   K +    +F  + T +W+F+K+  DD R G P S +D++ + +    + P       +R  +  P + +   K+  K   ++SK +P +  R+  +E++E  L+ +HPLA+YP+L   +P ++  +++  +DP+  +      EDA +      ++ ++
Sbjct:   19 PWYCNKRPSKCFAKCKHRRLRFPPMDTQNWVFVKEGMDDFRYGCP-SPEDTL-VCRRDEFLLPKI----SLRGPQADPKSGQ---KKLLKKAALFSKLSPAQLARKAFVEQVEAQLMAKHPLAMYPNLGEDMPPDLLLQVLKHLDPEREL------EDAWACCETQEKTTEV 175
[Close]
BLAST of Protein FAM47E vs. Ensembl Human
Match: FAM47A (family with sequence similarity 47 member A [Source:HGNC Symbol;Acc:HGNC:29962])

HSP 1 Score: 55.8398 bits (133), Expect = 1.455e-7
Identity = 43/173 (24.86%), Postives = 87/173 (50.29%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNKNQANHPKF--LLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESK-DIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLI-QHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKI 657
            PWY  +   K   K +    +F  + T +W+F+K+  DD R G P S +D++   +        F+  K  +R  +  P + +   K+  K   ++SK +P +  R+  +E++E  L+ +HPLA+YP+L   +P ++  +++  +DP+  +      EDA +      ++ ++
Sbjct:   19 PWYCNKRPSKCFAKCKHRRLRFPPMDTQNWVFVKEGMDDFRYGCP-SPEDTLVCRRD------EFLLPKISLRGPQADPKSGQ---KKLLKKAALFSKLSPAQLARKAFVEQVEAQLMAKHPLAMYPNLGEDMPPDLLLQVLKHLDPEREL------EDAWACCETQEKTTEV 175
[Close]
BLAST of Protein FAM47E vs. Ensembl Zebrafish
Match: zgc:158260 (zgc:158260 [Source:ZFIN;Acc:ZDB-GENE-061201-24])

HSP 1 Score: 121.709 bits (304), Expect = 2.883e-30
Identity = 117/408 (28.68%), Postives = 180/408 (44.12%), Query Frame = 1 
Query:  145 KFPWYKERLQLKYLN--KNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINP------NFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSK-TAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDV--------NVSNKSPIDSPKVTERFPPNAFKWISKKE---EKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDE 1308
            KFP ++E  + KY+   + +      L +  W FL    DD RD YP      V   + K +++P      N   S  ++R KL            SKN V YSK     +TQR++ I+ +E  L QHPLALYPHLE  +  E+FE+++ ++DPDM +   E+     +     +R  K +V        + S KSP D  K T     NA+KW   KE   E +  N  Q+++  +M           K FC W+  LGG+++++ E T+  LF S YE K TL++PI                        NK++   S  K + +     +HF+       ++ +K K        PK + + K+  H  +    N EL  + G   F EFI     R P+ + +
Sbjct:    5 KFPRFREHHKPKYIRDLRQKQQLSGSLDSHRWCFLSPGLDDFRDHYPTVY--KVPFTQCKCALSPVVFGLLNHACSVKLQRKKL------------SKNQVCYSKQNLQSQTQREF-IDAVEHKLKQHPLALYPHLESGMIPELFEQVLSVLDPDMCMKG-ELSLTKKTEKHLEDRRAKCEVSTQEILKRSTSIKSPKDESKDTR--ARNAYKWQELKEIDKEYQTANAKQSQEDKQMFV---------KFFCEWITSLGGETSDLRESTILDLFKSDYEKKTTLTLPI------------------------NKIAHQPS-GKCHSSVK---EHFKDHCVKELTEAYKPKILKTAYDSPKALADVKRNLH-FEPSKQNKELKQIHGIHAFREFIINKGVRMPRVISD 356
[Close]
BLAST of Protein FAM47E vs. Ensembl Xenopus
Match: stbd1 (family with sequence similarity 47 member E [Source:NCBI gene;Acc:100127812])

HSP 1 Score: 234.572 bits (597), Expect = 1.458e-71
Identity = 151/414 (36.47%), Postives = 222/414 (53.62%), Query Frame = 1 
Query:  136 SETKFPWYKERLQLKYLNKN--QANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKL--PPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSAS-----------EIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKE-EKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNV-SLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIED 1326
            S  + PWYKERL  K+L ++  + N      +  W FL    DD RDGYPP  D +   +       PN      I   +L  P +A++++ +RF+K    +SK  PL+  R+  I EIE GL QHPLALYPHLE  LP+E+FEE+V+IIDP+M + +S           E E D      +  +S K   ++S ++ + S +   +   N + W+SK E E  K ++    ++    A +E++K V KE+C+WV  LGG+  NI E T+ SLFASG++TKP LS+PIHV+EL ++PPELR +  + PT+        N            ++   +YGAWYL  K WKK+   E L+DPK     K    K  +   + EL  L G   F +F++    R P+FL+++   ED
Sbjct:   11 SRPRHPWYKERLHKKFLKESNPKLNFSGAFNSHHWKFLPKGLDDFRDGYPPPSDTTHSQL-------PNCTGPAPILNSRLNDPALAEKKLKQRFTKEQTCFSKLLPLQQARREYIAEIEYGLTQHPLALYPHLEEGLPAELFEEVVEIIDPEMRIRSSAGSYEHVLEEQEDEGDTSRHEQQETQSAK-SRDLSTRTSVMSEQSRSK---NPYVWLSKDEDECVKEDKKSKIKRSLTPALDENVKSVAKEYCNWVVSLGGEQCNINESTIFSLFASGFDTKPALSIPIHVVELNNVPPELRVSAGATPTQSALGAQRRNRREQYQEPIYQPSWMKTKYGAWYLDPKTWKKQTINESLKDPKDEDINKNVHSKGSLNPKDEELLQLYGTAAFKDFLKNRGYRSPEFLNKLFPEED 413
[Close]
BLAST of Protein FAM47E vs. Ensembl Mouse
Match: Fam47e (family with sequence similarity 47, member E [Source:MGI Symbol;Acc:MGI:2686227])

HSP 1 Score: 92.0485 bits (227), Expect = 8.763e-20
Identity = 99/396 (25.00%), Postives = 174/396 (43.94%), Query Frame = 1 
Query:  154 WYKERLQLK-YLNKNQANHPKFLLTDDWIFLKDCSDDCRDGYPP--SIDDSVG--IIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIE-----LTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
            WY   L  K ++ +N+     FL +  W+F+K+  D+ R   PP  S  D+    I +G  S  P                 KRQ      K   + SK   L    +  +E++E    QHPL LYP L  +LP+E+  ++++++DP+  +      ED  +    + + +K    +  K         +     + +W+ +    EK ++  +  K +++ ++ H + VT +FC W  DLG  S+ IEE  V   F  GY+T+ +    + +         + PP LR +    ++P  +  ++     +V           +YGAWYL +  WK++ A E L DP +    +    K ++      LA L+G   F +FI     R P+FL+++
Sbjct:   26 WYLGNLPPKRFIRRNRLTCQGFLNSRHWVFVKE--DNFRKDCPPHQSPKDAFLPLIHRGAPSATPE----------------KRQ--STLLKGATLLSK---LSKAGKAFLEDVEAEAAQHPLTLYPQLTEALPAELLLQVLEVLDPERKL------EDVWAYCQDTRKPMKEPTELVGKRSSQERPPKKTLISRSGQWLCE----EKSSKVDSLYKDRLLHDDVH-RGVT-DFCHWAEDLG--SSAIEEEFVLQQFDIGYQTRRSCDALLRLRLNQDKLFINRPPSLREHCGHWSEPRSSGRANPQKPKRV---------KMRYGAWYLNTSLWKRQRADEPLVDPMVSHKAQDSTFKEQLQEQGELLAGLRGTAAFKDFILSRGYRMPRFLEKI 375
[Close]
BLAST of Protein FAM47E vs. Ensembl Mouse
Match: Fam47c (family with sequence similarity 47, member C [Source:MGI Symbol;Acc:MGI:1918114])

HSP 1 Score: 89.7373 bits (221), Expect = 6.591e-19
Identity = 106/398 (26.63%), Postives = 176/398 (44.22%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNKNQANHPKFLLTDD---WIFLKDCSDDCRDGYPPSIDDSVGIIKG-KGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPS--EIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGS-----INKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
            PW+K+RL  K   K++    KF  + D   W+F+K+  DD R G PPS      II+G K    P    S ++          R+  K   ++  ++S  +  +  R+  ++  E  L Q PL  +P LE  +PS  E+ E +++ +D D  + A   + D      K   SV +  +V+ +S         RFP  + K+   +E+K    R +N  K  +     HI +   +FC+WV   G     I+E  +   F  GYE KP  +    + +++ +PP+LR    L +K  + + S   +     + K ++    N    +YGAWYL    WKK    E L DP+ +F  +  ++    I     +  L G   F +FI       P  L+++
Sbjct:   26 PWFKDRLPSKCFAKHKQEQHKFPTSLDGRRWVFVKEGLDDFRKGCPPS---EGMIIRGPKDGFLPTI--SHEVHH------GSRKSQKNRCQDLSLFSSLSLAQQARKAFVKRTEAKLTQRPLVFWP-LEDGIPSDIELLENVLEALDTDQKLEA---KVDHCEDRRKYIHSVHLP-SVAEESSEKEEPEPHRFP-GSRKYSMLQEKKP---RKKNPAKQSLYYH--HIPKGVYDFCAWVNSFG--DLGIDEHFMMKQFDIGYECKPVYTDSA-IKKISLLPPDLRFCRRL-SKVKEIRFSIQEANFERKLRKPFDPYKSNRDKIRYGAWYLKPYLWKKLVNDEPLMDPEELFELEGGKYSKPDI-----IEDLYGTIAFKDFILSKGYSMPAILEKL 392
[Close]
BLAST of Protein FAM47E vs. Ensembl Mouse
Match: Fam47e (family with sequence similarity 47, member E [Source:MGI Symbol;Acc:MGI:2686227])

HSP 1 Score: 70.4774 bits (171), Expect = 3.424e-13
Identity = 52/190 (27.37%), Postives = 90/190 (47.37%), Query Frame = 1 
Query:  757 EKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIE-----LTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
            EK ++  +  K +++ ++ H + VT +FC W  DLG  S+ IEE  V   F  GY+T+ +    + +         + PP LR +    ++P  +  ++     +V           +YGAWYL +  WK++ A E L DP +    +    K ++      LA L+G   F +FI     R P+FL+++
Sbjct:   57 EKSSKVDSLYKDRLLHDDVH-RGVT-DFCHWAEDLG--SSAIEEEFVLQQFDIGYQTRRSCDALLRLRLNQDKLFINRPPSLREHCGHWSEPRSSGRANPQKPKRV---------KMRYGAWYLNTSLWKRQRADEPLVDPMVSHKAQDSTFKEQLQEQGELLAGLRGTAAFKDFILSRGYRMPRFLEKI 233
[Close]
BLAST of Protein FAM47E vs. Ensembl Mouse
Match: Fam47e (family with sequence similarity 47, member E [Source:MGI Symbol;Acc:MGI:2686227])

HSP 1 Score: 51.9878 bits (123), Expect = 2.644e-7
Identity = 25/74 (33.78%), Postives = 39/74 (52.70%), Query Frame = 1 
Query: 1090 FQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
             +YGAWYL +  WK++ A E L DP +    +    K ++      LA L+G   F +FI     R P+FL+++
Sbjct:   99 MRYGAWYLNTSLWKRQRADEPLVDPMVSHKAQDSTFKEQLQEQGELLAGLRGTAAFKDFILSRGYRMPRFLEKI 172
[Close]
BLAST of Protein FAM47E vs. UniProt/SwissProt
Match: sp|Q6ZV65|FA47E_HUMAN (Protein FAM47E OS=Homo sapiens OX=9606 GN=FAM47E PE=2 SV=3)

HSP 1 Score: 103.605 bits (257), Expect = 7.656e-23
Identity = 101/386 (26.17%), Postives = 168/386 (43.52%), Query Frame = 1 
Query:  187 NKNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGI-IKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNK-------SPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDD---NKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEV 1311
            +KN    P  L +   +F +   DD R G PP       + ++G       F+     R  +L P  KRQ+  +  K   V SK +P +  R+  +E++E  L  HPLALY +LE ++P E+  ++++++DPD  +      ED  +    + + +K    +  K        P     V+     NA +W+   EEK          K+ ++ EN     + +   S          +I+E  +   F   YETKP+    +H ++L  +P EL+ +V L    +     K+     + K        +   +YGAWYL  K WKK+   E L DP++    ++E  K ++      LA L G   F +FI     R P+FL+ +
Sbjct:   30 HKNGLKFPTSLHSRQLVFPRKGLDDFRKGCPPCTGLVTQVPVEG-------FLPQIYHRAPQLAP-KKRQI--KLLKEADVLSKLSPAQQARKAFLEDVEAHLTPHPLALYLNLEEAMPIELLSKVLEVLDPDRKL------EDTWAYCQDTRKGMKEPTKLLKKHSTQVYLGPSKKTSVS-----NAGQWLY--EEKP--------HKMDLLHENGPRPGLHENGIS----------DIDEEFILKQFDIDYETKPSHDA-LHTMKLNQVPLELKRSVGLSKLQETEFFQKLGYERKLQKPQNPYKPKWVKMRYGAWYLNPKLWKKQRVDEPLVDPEVSHKAQEENFKKELQEQEELLADLHGTVAFKDFILSRGYRTPRFLENM 373
[Close]
BLAST of Protein FAM47E vs. UniProt/SwissProt
Match: sp|Q5JRC9|FA47A_HUMAN (Protein FAM47A OS=Homo sapiens OX=9606 GN=FAM47A PE=2 SV=3)

HSP 1 Score: 55.8398 bits (133), Expect = 6.988e-7
Identity = 43/173 (24.86%), Postives = 87/173 (50.29%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNKNQANHPKF--LLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESK-DIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLI-QHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKI 657
            PWY  +   K   K +    +F  + T +W+F+K+  DD R G P S +D++   +        F+  K  +R  +  P + +   K+  K   ++SK +P +  R+  +E++E  L+ +HPLA+YP+L   +P ++  +++  +DP+  +      EDA +      ++ ++
Sbjct:   19 PWYCNKRPSKCFAKCKHRRLRFPPMDTQNWVFVKEGMDDFRYGCP-SPEDTLVCRRD------EFLLPKISLRGPQADPKSGQ---KKLLKKAALFSKLSPAQLARKAFVEQVEAQLMAKHPLAMYPNLGEDMPPDLLLQVLKHLDPEREL------EDAWACCETQEKTTEV 175
[Close]
BLAST of Protein FAM47E vs. UniProt/SwissProt
Match: sp|A6NHR8|FA47D_HUMAN (Putative protein FAM47D OS=Homo sapiens OX=9606 GN=FAM47DP PE=5 SV=3)

HSP 1 Score: 51.9878 bits (123), Expect = 6.391e-6
Identity = 82/396 (20.71%), Postives = 162/396 (40.91%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNKNQANHPKF--LLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEF--CSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQ----HFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVA 1314
            PWY ++   KY+ K +    +F  + T +W+F+K+  D    G P   D  +  +         F+  K   R        RQ  K+  K   ++SK  P +   +  +EE +L + +HPLA+YP+L   +P ++  +++ ++DP+  +      E A +      +++K     +   P  +P       P   +    KE    L +       + ++++   +  +++     W  D+G     ++E ++ +LF    + + T      + ++     EL+  + L  + D+ + S     ++  + A  ++       +YG WYL  K  KK  + + L DPKL+     E            L  L GP  F +FI       P  ++ + 
Sbjct:   19 PWYCDKPPSKYIAKRKHRRLRFPPMDTQNWVFVKESMDSFHYGCPSPEDMLICRLN-------EFLLPKISHRGPQADPKSRQ--KKLLKKVALFSKLLPAQPAWKAFVEEAQL-MAKHPLAMYPNLGEDMPPDLLLQMLKLLDPERKL------EKAWAYCEGREKTIK---EPTKPEPPKAPVSHHFLEPPKIRASCLKE----LLQEDTPSTTECVSDSLQHRYTSRKMHDFKWARDMG-----VDEESIRNLFDFTPKWRATYED-QQIKKIKEWVSELQYRIKL-DEMDEVESSQEKDWDRKLQMAPNSYTAQCVKMRYGVWYLKPKLGKKLRSDQPLIDPKLLLEKPDEPDI---------LDDLYGPIAFKDFILSKGYEMPGIIERLC 375
[Close]
BLAST of Protein FAM47E vs. TrEMBL
Match: A0A1S3IVV8 (protein FAM47E OS=Lingula unguis OX=7574 GN=LOC106167962 PE=4 SV=1)

HSP 1 Score: 305.064 bits (780), Expect = 1.355e-95
Identity = 178/408 (43.63%), Postives = 257/408 (62.99%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNK-NQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISK-KEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKS--NNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLP-TKPDDNKVSDNGSINKVYETASGNFQ----HFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTN-KRKPKFLDEVAEIEDQIEKEK 1344
            PWYKER+  KY+      +  K L+  DW+F+KD  DD RDG PP +DD   +IK      PN + S+     K P  A+R   +RF+K +V YS+  P   +R+ +I+EIE GL QHPLALYPHLE  +P E+FEE+VDI+DP+MNV   +  +D     S+S +S  +D  V  +   +  ++ +    N ++W+ + +E+++K  + Q +++    +++EHIK VTK+FC WVA LGG+S  NNIEE T+TSLFASGYETKP LSVPIHV+ELT++PPELR + ++P T+  +NK        K     SGN++      +YGAWYLP+K W+K+P  E LQDPK + + ++ E K K    + ELA L G K F +FI++    RKP+FL  VAEI+ +  +E+
Sbjct:   23 PWYKERIYTKYIKSLKPTDTSKMLIGKDWMFIKDGLDDFRDGLPPQVDDGDIMIKSSKGPAPNLMGSEHSTPVKQP--AQR---RRFNKYEVCYSRVTPQMQKRREHIDEIEYGLTQHPLALYPHLEECMPPELFEEVVDILDPEMNVDPGDESDD-----SESEKSESMDQKVDEEPQNEQEEMEDDRIRNPYRWLPRKEEKEKKDKKKQTDKRPSSPSQDEHIKTVTKDFCDWVAGLGGESNKNNIEESTITSLFASGYETKPALSVPIHVVELTNVPPELRQSAAVPLTQQQNNKNLPTAEQVKKDWIHSGNYEPSWVPHKYGAWYLPTKLWQKRPVDEPLQDPKALKDLEKSETKKKSNEKDHELAPLHGSKAFMDFIDRKKVVRKPEFLIHVAEIQRKAAEEE 420
[Close]
BLAST of Protein FAM47E vs. TrEMBL
Match: V4A8C9 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_150620 PE=4 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 7.958e-90
Identity = 173/401 (43.14%), Postives = 248/401 (61.85%), Query Frame = 1 
Query:  157 YKERLQLKYLNK-NQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIE--EDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTK---------PDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIE 1323
            YKERL+ KY+      +  K L+  DW+FLKD  DD RDG PP        IKG    +P  I S +     + P+ +  V  RF K  V +S+  P + Q++ +IEEIE GL+QHPLALYPHLE S+P+E+FE++VDI+DP+MN+ + E +  +D  ++    N    ID   S K        + R   N ++WIS+K+EK+  N+ +NE++    AE EHI+ VTK+FC+WVADLGG+SNNIEE T+TSLFASGYETKP LSVPIHV+ELT++PPELR +  +  +         P + + +     +  YE +      F+YG+WYLP K WKK+   ++L DPK + N +  + K K  SLN +L+++ G + F EFI +   RK +FL++V+EI+
Sbjct:   14 YKERLRTKYIKSLTPGDTSKCLIGKDWMFLKDGLDDYRDGLPPLAKGEEVFIKGDKGPSPCIIGSSE----SIEPVKRPAVKNRFDKYQVCFSRITPQQQQKREHIEEIEYGLLQHPLALYPHLEESVPTELFEDVVDILDPEMNIDSEEEDSIKDDSTIRDAENLHKSIDTPSSAKKTEAESDNSFR---NCYRWISRKDEKKD-NKKKNERRPSSPAEKEHIENVTKDFCNWVADLGGESNNIEESTITSLFASGYETKPALSVPIHVVELTNVPPELRMSAPVQAQAQTQNTKNLPAEEEKTKPWKYSGAYEPSC---VKFKYGSWYLPPKTWKKREWNDQLMDPKELKNQEMSDSKKKSQSLNEDLSNMHGAEAFMEFINRKGLRKIEFLEQVSEIQ 403
[Close]
BLAST of Protein FAM47E vs. TrEMBL
Match: A0A267DPR8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005114g4 PE=4 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 9.696e-90
Identity = 164/426 (38.50%), Postives = 246/426 (57.75%), Query Frame = 1 
Query:  148 FPWYKERLQLKYLNKNQANHP-KFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKI---------DVNVSNKSPIDSPKVTERFPPNAF-KWISKKEEKEKLNRAQNEQ-KLKM-IAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVY---------ETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIEDQIEKEKMQSKE 1359
             PWYKER+ LKY       +P K  +  DW+F+KD  DD RDG PP  + SV I      + P              PI +     R++K ++ +SK  PL+ +++ +I E+E GL+QHPLALYPHLE S+P E++++IVD++DP+M +  +E  ++A  +S+       I         D N S  +  +  K ++      F  WI K+EE       ++   +L+   A++ HI++VTK+FC WVA LGG++NNIEE TV SLFASGYETKP LSVPIHV+ELTSIPPELR N++LP  P  ++  +   + + Y         ++    +  F+YGAWYL  + W+K PAG+ L+DP+   + KQ E + +   +N  L+ L G + F +F++K + RKP+FL +V  I    E E+ + +E
Sbjct:   18 IPWYKERINLKYSKATGPGNPGKVNVGKDWVFVKDGLDDFRDGLPPITEGSVVIRNPNAGLGPAL----KSHNTGATPIKQSVNDGRYTKEEICFSKQTPLQRKQRQHISELEYGLVQHPLALYPHLEESVPPEVYDDIVDLLDPEMTLLETEGVDEATLLSADGQHQASIRHQEVQTSADSNGSGGASGERAKSSKSADGRHFYSWIKKREEAAATAAGRDADARLRQEPAKDPHIEKVTKDFCEWVASLGGEANNIEESTVMSLFASGYETKPALSVPIHVVELTSIPPELRQNIALP--PQRHQDREEAEMEREYLRRLGKEAGDSTDPGWMKFRYGAWYLEPETWRKLPAGQPLEDPEERKDTKQLEARQRAQEMNETLSSLHGARSFIDFMKKKDTRKPEFLTQVEHIIAHREAEEARRQE 437
[Close]
BLAST of Protein FAM47E vs. TrEMBL
Match: A0A1I8HU61 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 5.155e-89
Identity = 161/413 (38.98%), Postives = 240/413 (58.11%), Query Frame = 1 
Query:  148 FPWYKERLQLKYLNKNQANHP-KFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKI---------DVNVSNKSPIDSPKVTERFPPNAF-KWISKKEEKEKLNRAQNEQ-KLKM-IAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVY---------ETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEI 1320
             PWYKER+ LKY       +P K  +  DW+F+KD  DD RDG PP  + SV I      + P              PI +     R++K ++ +SK  PL+ +++ +I E+E GL+QHPLALYPHLE S+P E++++IVD++DP+M +  +E  ++A  +S+       I         D N S  +  +  K ++      F  WI K+EE       ++   +L+   A++ HI++VTK+FC WVA LGG++NNIEE TV SLFASGYETKP LSVPIHV+ELTSIPPELR N++LP  P  ++  +   + + Y         ++    +  F+YGAWYL  + W+K PAG+ L+DP+   + KQ E + +   +N  L+ L G + F +F++K + RKP+FL +V  I
Sbjct:   22 IPWYKERINLKYSKATGPGNPGKVNVGKDWVFVKDGLDDFRDGLPPITEGSVVIRNPNAGLGPAL----KSHNTGATPIKQSVNDGRYTKEEICFSKQTPLQRKQRQHISELEYGLVQHPLALYPHLEESVPPEVYDDIVDLLDPEMTLLETEGVDEATLLSADGQHQASIRHQEVQTSADSNGSGGASGERAKSSKSADGRHFYSWIKKREEAAATAAGRDADARLRQEPAKDPHIEKVTKDFCEWVASLGGEANNIEESTVMSLFASGYETKPALSVPIHVVELTSIPPELRQNIALP--PQRHQDREEAEMEREYLRRLGKEAGDSTDPGWMKFRYGAWYLEPETWRKLPAGQPLEDPEERKDTKQLEARQRAQEMNETLSSLHGARSFIDFMKKKDTRKPEFLTQVEHI 428
[Close]
BLAST of Protein FAM47E vs. TrEMBL
Match: R7V2T3 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_228322 PE=4 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 5.583e-89
Identity = 173/402 (43.03%), Postives = 243/402 (60.45%), Query Frame = 1 
Query:  151 PWYKERLQLKYLNK-NQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASE----------IEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQN-EQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEI 1320
            PWYKERL+ KY+      +  KFL   +W F+KD  DD RDG PP + +   I   KG ++PN   S    +    P A+R    RF+K+++ YS+  PL+ QR+ +I EIE GL QHPLALYPHLE  LP ++FE++VDI+DP+MN+++ E           E D+ +  S  N    I    S K    +    E    N ++W+ KK+EKEK ++ +  E++    +E+ HIK VTKEFC WV+ LGG SNNIEE T+TSLFASGYETKP LSVPIHV+ELT++PPELR + S+P      K  +N   +K       ++  F+YGAWYL  + W+K  A E L+DPK + + +  E K K  +L+ +LA + G K F +FI++   R+P+FL+ V  I
Sbjct:   23 PWYKERLRTKYIKSLGPRDTSKFLNGKNWTFMKDGLDDFRDGLPPPLPNGCMIEANKG-LSPNICGSSADLKSVKQPAARR----RFNKHEICYSRVTPLQQQRRDHIGEIEYGLTQHPLALYPHLEECLPPDMFEDVVDILDPEMNLASDEEDEEIFEELDAERDSHA-DSLGNTGFIIPDRGSYKDDESNDGSEEVKVRNPYRWLPKKDEKEKKDKKKTAERRNHSPSEDSHIKNVTKEFCDWVSGLGGDSNNIEESTITSLFASGYETKPALSVPIHVVELTNVPPELRMSASVPPSQHTQKTDENKLKDKTQSAYEPSWLKFRYGAWYLKPQSWRKMRADEPLRDPKELKDDQMSEAKKKSANLDFDLAPMHGAKSFMDFIDRKATREPEFLNRVKTI 418
[Close]
BLAST of Protein FAM47E vs. Ensembl Cavefish
Match: zgc:158260 (family with sequence similarity 47 member E [Source:NCBI gene;Acc:103046655])

HSP 1 Score: 176.407 bits (446), Expect = 5.303e-50
Identity = 132/419 (31.50%), Postives = 203/419 (48.45%), Query Frame = 1 
Query:  151 PWYKERLQLKYLN----KNQANHPKFLLTD-DWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNV---------SASEI--EEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIEDQIEKEKMQSKE 1359
            PWYKERL+ K+L     K Q        TD  W FL    DD RDGYP ++ D V   + +  I P      +  R   P      + KRF+K    YS+   L+  R+  +E +E  L  HPLALYPHL   L  E+FE+++ ++DP+M V         S  E+  EE+  +       + + +  +S  +   + +V      N +K     + + K N+  +   L   A++E I EVTK FC WVA LGG+++++ E T+ SLF  G+E KP++  P+HV       P+   + S P   D         +NKVY + +       YGAWYL  K WKK+PA E L+DP +  +    E + +    + EL  + G + F EFI     R P+FL  +   E++ ++ + +  +
Sbjct:   19 PWYKERLRTKFLKDPLKKKQQQCGALDATDRGWRFLNPGQDDFRDGYP-ALQDEV-FTQPQCRIAPPLFGLVNQAR---PATV---LKKRFNKEQACYSRINCLRQTRRQYVEAVEQKLSSHPLALYPHLTSGLQPELFEDVLSVLDPEMCVERESDVISFSKEEVSREENVATCEGPEQETSREETKLSIGTVSSADEVKGCSSRNPYKLPETMDTQPKGNQMVSNTFLPSPAQDEDITEVTKLFCDWVALLGGETSSLTESTIHSLFFCGFEKKPSIRSPVHV-------PKDDSSRSAPKLKDPE-------LNKVYPSNTKT----TYGAWYLDPKTWKKRPASEPLRDPSVTEDL---ELELQPTEKDKELKEIHGTQAFREFIISKGLRMPRFLSSLFPSEEEEQEHRTRGTD 408
[Close]
BLAST of Protein FAM47E vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003541.1 (pep scaffold:Pmarinus_7.0:GL479924:9210:16916:1 gene:ENSPMAG00000003235.1 transcript:ENSPMAT00000003541.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 221.476 bits (563), Expect = 2.204e-67
Identity = 124/339 (36.58%), Postives = 192/339 (56.64%), Query Frame = 1 
Query:  430 SKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPP-----------NAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQ-----YGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVA-EIEDQIEKEKMQSKEEDKN*IVNRNKH 1395
            S+  PL+  R+  +EE E GL +HPLALYPHL+ SL  E+FEE+V+++DP+M    + ++ED      +      +D N + +     P   E  P            N ++W+ K E   K +R    +     +++E +K VT +FC WVA LGG+S+ IEE T+ SLFASGYETKP L+VPIHV+EL ++PPELR   S      D+  S N +    ++T +G  Q  Q     YGAWYLP   WKKKP  E L+DP ++   +  E K +  +++T+L HL G   F +F+++   R+P+FL++V+ E+ ++  +E+  + +  K   V + + 
Sbjct:  105 SRLTPLQQARRALVEETECGLREHPLALYPHLQQSLDPELFEEVVNLLDPEMRRFEASLQEDVEPPPVEQEDEEDMD-NANGEDTKPEPYPIEDRPTTISSTREGRYQNPYRWLPKVEGSSKEDRRGRRKPPSGSSQDEDLKRVTHDFCDWVASLGGESDVIEESTLMSLFASGYETKPPLTVPIHVVELANVPPELR---SAAGATPDSAPSANANGTSAHKTGAGPQQQPQSGRMCYGAWYLPPATWKKKPQNEPLEDPNVVQEREASEAKKRATAIDTQLLHLHGALAFRKFVDEKGARRPQFLEQVSMELSEEDGREQTSAGKARKRQTVEKGRQ 439
[Close]
BLAST of Protein FAM47E vs. Ensembl Nematostella
Match: EDO35420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLJ7])

HSP 1 Score: 287.73 bits (735), Expect = 8.811e-93
Identity = 169/419 (40.33%), Postives = 242/419 (57.76%), Query Frame = 1 
Query:   88 MSHCYRPLTFTEFKKYSETKFPWYKERLQLKYLN-KNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNV-----------SASEIEEDAMSVSSKSNRSVK-IDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQ-NEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFL 1302
            M+H Y  L     K   +   PWYKERLQ KY+  + +      L+   W FL+  SDD RDG PP    +  + +      P    S+     K       Q  K+F+K + VYSK  PL+ QR+ ++E+IE  L QHPLAL+PHLE SLP ++FE++V ++DP+MN+           +  + EEDA   S + ++S K +D   S++S     + ++R     +KW+ K +E +K  RA+ + +K      +  I++VT+EFCSWVA LGG+SNNIEE TV SLFASGYETKP LSVPIHV+ELT++PPELR N   P+KP D +++D  +  K +     ++   +YGAWYL  K WK +P  E+L DPK   +    E K K   L+  LA + G KL  +F+++ N R P+FL
Sbjct:    1 MAHKYDLLLVKTQKDDKKRLQPWYKERLQTKYIQPRCKRGKSDTLVGSQWTFLERGSDDFRDGLPPEAFYNY-VKRNDLGFGPTLTGSEQSYDGK-------QEMKKFTKTEAVYSKALPLQQQRREHVEDIEFNLTQHPLALFPHLEESLPPDVFEDVVGVLDPEMNIDSDAEDDTFEDTTEDSEEDAKPASVRGSQSAKSMDSKQSSES-----RQSKR---PTYKWLPKHDENQKEERARASRKKPDSPTTDTRIEDVTREFCSWVAGLGGESNNIEESTVMSLFASGYETKPALSVPIHVVELTNVPPELRMNTGSPSKPSDTRIADT-TTTKPFPGYQPSWVKVKYGAWYLNPKTWKARPDQEQLLDPKEQQDKTLSEAKVKSQKLDAVLATMHGAKLLKDFVDRKNTRVPEFL 402
[Close]
BLAST of Protein FAM47E vs. Planmine SMEST
Match: SMESG000026097.1 (SMESG000026097.1)

HSP 1 Score: 871.692 bits (2251), Expect = 0.000e+0
Identity = 426/428 (99.53%), Postives = 428/428 (100.00%), Query Frame = 1 
Query:   88 MSHCYRPLTFTEFKKYSETKFPWYKERLQLKYLNKNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIEDQIEKEKMQSKEEDKN 1371
            MSHCYRPLTFTEFKKYSETKFPWYKERLQLKYLNKNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVK+DVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGG+SNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIEDQIEKEKMQSKEEDKN
Sbjct:    1 MSHCYRPLTFTEFKKYSETKFPWYKERLQLKYLNKNQANHPKFLLTDDWIFLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAKRQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSLPSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKLDVNVSNKSPIDSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKEFCSWVADLGGQSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPELRGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKKPAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKRKPKFLDEVAEIEDQIEKEKMQSKEEDKN 428
The following BLAST results are available for this feature:
BLAST of Protein FAM47E vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FAM47E1.594e-2326.17family with sequence similarity 47 member E [Sourc... [more]
FAM47E-STBD13.323e-1825.57FAM47E-STBD1 readthrough [Source:HGNC Symbol;Acc:H... [more]
FAM47E2.524e-1625.18family with sequence similarity 47 member E [Sourc... [more]
FAM47A1.338e-724.42family with sequence similarity 47 member A [Sourc... [more]
FAM47A1.455e-724.86family with sequence similarity 47 member A [Sourc... [more]
back to top
BLAST of Protein FAM47E vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Protein FAM47E vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Protein FAM47E vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1582602.883e-3028.68zgc:158260 [Source:ZFIN;Acc:ZDB-GENE-061201-24][more]
back to top
BLAST of Protein FAM47E vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
stbd11.458e-7136.47family with sequence similarity 47 member E [Sourc... [more]
back to top
BLAST of Protein FAM47E vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Fam47e8.763e-2025.00family with sequence similarity 47, member E [Sour... [more]
Fam47c6.591e-1926.63family with sequence similarity 47, member C [Sour... [more]
Fam47e3.424e-1327.37family with sequence similarity 47, member E [Sour... [more]
Fam47e2.644e-733.78family with sequence similarity 47, member E [Sour... [more]
back to top
BLAST of Protein FAM47E vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q6ZV65|FA47E_HUMAN7.656e-2326.17Protein FAM47E OS=Homo sapiens OX=9606 GN=FAM47E P... [more]
sp|Q5JRC9|FA47A_HUMAN6.988e-724.86Protein FAM47A OS=Homo sapiens OX=9606 GN=FAM47A P... [more]
sp|A6NHR8|FA47D_HUMAN6.391e-620.71Putative protein FAM47D OS=Homo sapiens OX=9606 GN... [more]
back to top
BLAST of Protein FAM47E vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3IVV81.355e-9543.63protein FAM47E OS=Lingula unguis OX=7574 GN=LOC106... [more]
V4A8C97.958e-9043.14Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A267DPR89.696e-9038.50Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8HU615.155e-8938.98Uncharacterized protein OS=Macrostomum lignano OX=... [more]
R7V2T35.583e-8943.03Uncharacterized protein OS=Capitella teleta OX=283... [more]
back to top
BLAST of Protein FAM47E vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1582605.303e-5031.50family with sequence similarity 47 member E [Sourc... [more]
back to top
BLAST of Protein FAM47E vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000003541.12.204e-6736.58pep scaffold:Pmarinus_7.0:GL479924:9210:16916:1 ge... [more]
back to top
BLAST of Protein FAM47E vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Protein FAM47E vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO354208.811e-9340.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Protein FAM47E vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Protein FAM47E vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000026097.10.000e+099.53SMESG000026097.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019223 ID=SMED30019223|Name=Protein FAM47E|organism=Schmidtea mediterranea sexual|type=transcript|length=1400bp
TGTAAATTTATTTTATTTGAATTTTATTAAATTTTCATATTTGCTAAACT
TATTGATATTTGTAATAAACATTGTTATTTATTTTTAATGTCGCACTGTT
ACCGACCTCTGACATTTACTGAATTCAAAAAATATTCCGAAACTAAATTT
CCATGGTACAAAGAAAGACTTCAGTTAAAATATTTAAATAAAAATCAAGC
TAATCATCCAAAATTTTTATTAACTGACGATTGGATTTTTTTAAAGGATT
GTAGTGATGATTGTAGGGATGGCTATCCACCATCAATCGATGATTCCGTT
GGAATTATAAAAGGTAAAGGAAGTATAAATCCAAATTTTATAGAATCAAA
GGATATTCGAAGAGATAAACTGCCACCAATCGCAAAACGTCAAGTACCTA
AACGATTTTCCAAAAACGATGTGGTTTATTCCAAAACTGCACCTTTGAAA
ACTCAGCGTCAATATAACATCGAAGAAATAGAATTGGGTCTTATTCAACA
CCCACTGGCACTTTATCCTCATTTAGAATTATCTTTACCTTCTGAGATAT
TTGAGGAGATCGTCGACATCATAGATCCTGATATGAATGTTTCTGCATCG
GAAATTGAAGAAGATGCTATGTCTGTGAGTTCCAAGTCAAATAGATCGGT
GAAAATTGACGTCAATGTATCTAATAAATCTCCCATTGACAGTCCGAAAG
TCACAGAAAGATTCCCACCAAATGCTTTTAAATGGATTTCTAAGAAGGAA
GAGAAAGAAAAATTAAATCGGGCTCAGAATGAACAAAAACTCAAAATGAT
AGCAGAGAATGAACACATCAAAGAAGTTACTAAAGAATTCTGTTCTTGGG
TAGCTGATTTAGGAGGTAAGTCAAATAATATTGAAGAGCCAACTGTTACG
AGCCTCTTCGCCAGTGGTTATGAAACTAAACCAACCTTGTCGGTCCCGAT
TCATGTCATTGAGCTAACTAGTATTCCACCAGAGTTAAGAGGTAACGTTA
GTCTTCCAACGAAACCAGATGACAACAAAGTTTCGGATAATGGTTCCATA
AATAAAGTTTATGAAACCGCAAGTGGAAATTTCCAACACTTCCAATATGG
TGCTTGGTATCTACCAAGTAAGTTCTGGAAGAAAAAGCCTGCTGGTGAAA
AATTGCAAGATCCGAAACTTATGTTCAATTTCAAACAAGAAGAGCATAAG
GCCAAAGTTATATCACTCAATACAGAATTGGCACATTTGCAGGGTCCTAA
ATTATTCAGTGAATTTATTGAAAAAACTAATAAAAGAAAACCCAAATTTC
TTGACGAGGTCGCTGAAATAGAAGATCAAATTGAAAAAGAAAAAATGCAA
TCAAAAGAAGAAGATAAGAACTAAATTGTTAATAGAAATAAACATTTCTG
back to top

protein sequence of SMED30019223-orf-1

>SMED30019223-orf-1 ID=SMED30019223-orf-1|Name=SMED30019223-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=429bp
MSHCYRPLTFTEFKKYSETKFPWYKERLQLKYLNKNQANHPKFLLTDDWI
FLKDCSDDCRDGYPPSIDDSVGIIKGKGSINPNFIESKDIRRDKLPPIAK
RQVPKRFSKNDVVYSKTAPLKTQRQYNIEEIELGLIQHPLALYPHLELSL
PSEIFEEIVDIIDPDMNVSASEIEEDAMSVSSKSNRSVKIDVNVSNKSPI
DSPKVTERFPPNAFKWISKKEEKEKLNRAQNEQKLKMIAENEHIKEVTKE
FCSWVADLGGKSNNIEEPTVTSLFASGYETKPTLSVPIHVIELTSIPPEL
RGNVSLPTKPDDNKVSDNGSINKVYETASGNFQHFQYGAWYLPSKFWKKK
PAGEKLQDPKLMFNFKQEEHKAKVISLNTELAHLQGPKLFSEFIEKTNKR
KPKFLDEVAEIEDQIEKEKMQSKEEDKN*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR032743FAM47
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 394..428
NoneNo IPR availablePANTHERPTHR46449:SF5ZGC:158260coord: 20..425
NoneNo IPR availablePANTHERPTHR46449FAMILY NOT NAMEDcoord: 20..425
IPR032743FAM47 familyPFAMPF14642FAM47coord: 21..177
e-value: 5.4E-9
score: 36.0