SMED30019210
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019210 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Alignments
SMED30019210 aligns in the following genomic locations:
Homology
BLAST of SMED30019210 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 268.085 bits (684), Expect = 1.769e-85 Identity = 151/162 (93.21%), Postives = 156/162 (96.30%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKGANTNEIGALEYKKNXXXXXXXXXGFNNPKGYCYLNC 487 QLKMEDSNSS EK+ LELNRS+ ++K LQ ENNNIKSGFASQIE+LELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTK ANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC Sbjct: 630 QLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKAANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC 791 HSP 2 Score: 53.5286 bits (127), Expect = 1.136e-8 Identity = 35/84 (41.67%), Postives = 57/84 (67.86%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENE 253 Q++++DSN S E+LKLEL +S+++++ LQ++NNNI+S FA QIE+L+ + E + + LQ ++ K + NLQ ENE Sbjct: 531 QMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENE 614 HSP 3 Score: 45.4394 bits (106), Expect = 5.677e-6 Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQ-----------IEKLELKMN------EINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSS------------NNHAECKLKFNQSETEKSILIAE 337 + ++++ N S++ LK +LN+ ++ K LQDEN++IKS S+ IE++ KM E++E N+IL +NY + L +EN+KL+S+ N + E KLKFNQS EK L +E Sbjct: 295 EFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKSELESRKNCDSPTKNANIEQIIFKMKNLNLNEEMDERNQILLHNYDERYL-----------RENKKLESTIKEIEKKNKILQNKYEEYKLKFNQSNHEKQNLTSE 424
BLAST of SMED30019210 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 268.085 bits (684), Expect = 1.798e-85 Identity = 151/162 (93.21%), Postives = 156/162 (96.30%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKGANTNEIGALEYKKNXXXXXXXXXGFNNPKGYCYLNC 487 QLKMEDSNSS EK+ LELNRS+ ++K LQ ENNNIKSGFASQIE+LELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTK ANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC Sbjct: 625 QLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKAANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC 786 HSP 2 Score: 53.5286 bits (127), Expect = 1.113e-8 Identity = 35/84 (41.67%), Postives = 57/84 (67.86%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENE 253 Q++++DSN S E+LKLEL +S+++++ LQ++NNNI+S FA QIE+L+ + E + + LQ ++ K + NLQ ENE Sbjct: 526 QMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENE 609 HSP 3 Score: 45.4394 bits (106), Expect = 5.673e-6 Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQ-----------IEKLELKMN------EINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSS------------NNHAECKLKFNQSETEKSILIAE 337 + ++++ N S++ LK +LN+ ++ K LQDEN++IKS S+ IE++ KM E++E N+IL +NY + L +EN+KL+S+ N + E KLKFNQS EK L +E Sbjct: 290 EFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKSELESRKNCDSPTKNANIEQIIFKMKNLNLNEEMDERNQILLHNYDERYL-----------RENKKLESTIKEIEKKNKILQNKYEEYKLKFNQSNHEKQNLTSE 419
BLAST of SMED30019210 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 268.085 bits (684), Expect = 3.685e-85 Identity = 151/162 (93.21%), Postives = 156/162 (96.30%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKGANTNEIGALEYKKNXXXXXXXXXGFNNPKGYCYLNC 487 QLKMEDSNSS EK+ LELNRS+ ++K LQ ENNNIKSGFASQIE+LELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTK ANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC Sbjct: 630 QLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKAANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC 791 HSP 2 Score: 53.9138 bits (128), Expect = 8.266e-9 Identity = 35/84 (41.67%), Postives = 57/84 (67.86%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENE 253 Q++++DSN S E+LKLEL +S+++++ LQ++NNNI+S FA QIE+L+ + E + + LQ ++ K + NLQ ENE Sbjct: 531 QMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENE 614 HSP 3 Score: 45.8246 bits (107), Expect = 4.341e-6 Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQ-----------IEKLELKMN------EINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSS------------NNHAECKLKFNQSETEKSILIAE 337 + ++++ N S++ LK +LN+ ++ K LQDEN++IKS S+ IE++ KM E++E N+IL +NY + L +EN+KL+S+ N + E KLKFNQS EK L +E Sbjct: 295 EFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKSELESRKNCDSPTKNANIEQIIFKMKNLNLNEEMDERNQILLHNYDERYL-----------RENKKLESTIKEIEKKNKILQNKYEEYKLKFNQSNHEKQNLTSE 424
BLAST of SMED30019210 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1) HSP 1 Score: 267.7 bits (683), Expect = 4.319e-85 Identity = 151/162 (93.21%), Postives = 156/162 (96.30%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKGANTNEIGALEYKKNXXXXXXXXXGFNNPKGYCYLNC 487 QLKMEDSNSS EK+ LELNRS+ ++K LQ ENNNIKSGFASQIE+LELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTK ANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC Sbjct: 625 QLKMEDSNSSNEKMLLELNRSEYKFKSLQIENNNIKSGFASQIEQLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSSNNHAECKLKFNQSETEKSILIAEKSPESQLFIDSTKAANTNEIGALEYKKNIPVIPVPPIGFNNPKGYCYLNC 786 HSP 2 Score: 53.9138 bits (128), Expect = 8.182e-9 Identity = 35/84 (41.67%), Postives = 57/84 (67.86%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLNQSEIDKINLQKENE 253 Q++++DSN S E+LKLEL +S+++++ LQ++NNNI+S FA QIE+L+ + E + + LQ ++ K + NLQ ENE Sbjct: 526 QMQIKDSNYSNERLKLELKQSENKFQSLQNDNNNIESRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENE 609 HSP 3 Score: 45.8246 bits (107), Expect = 4.770e-6 Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQ-----------IEKLELKMN------EINEENKILQNNYSQSKLNQSEIDKINLQKENEKLKSS------------NNHAECKLKFNQSETEKSILIAE 337 + ++++ N S++ LK +LN+ ++ K LQDEN++IKS S+ IE++ KM E++E N+IL +NY + L +EN+KL+S+ N + E KLKFNQS EK L +E Sbjct: 290 EFQLDNLNRSVKNLKSQLNKMGNKCKDLQDENDHIKSELESRKNCDSPTKNANIEQIIFKMKNLNLNEEMDERNQILLHNYDERYL-----------RENKKLESTIKEIEKKNKILQNKYEEYKLKFNQSNHEKQNLTSE 419
BLAST of SMED30019210 vs. Planmine SMEST
Match: SMESG000038519.1 (SMESG000038519.1) HSP 1 Score: 115.931 bits (289), Expect = 1.513e-31 Identity = 83/189 (43.92%), Postives = 111/189 (58.73%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYSQSKLN-----------QSEIDKINLQKENEKLKSS-NNHAECKLKFNQSETEKSI---------LIAEKSPESQLFIDSTK------GANTNEIGALEYKKNXXXXXXXXXGFNNPKGYCYLNC 487 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKS FASQIE+LE + +INEE +ILQN Y + KL Q + + NL + +KL+S +N + ++K + E + ++ L + + S+ ++ + G+ +N Y I PIGF NPKG+C+LNC Sbjct: 118 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSAFASQIEQLESIIKQINEERQILQNKYDECKLRFNQSNHEEQILQWQTEMKNLTTKYDKLQSDRDNEFQAQIKREKREIQSALNAVLKENDRLNSRINSRSRSNLEVKRRDRRSFGSESNMSNVTPYNYQNNNILRDPIGFENPKGFCFLNC 306 HSP 2 Score: 62.003 bits (149), Expect = 8.451e-12 Identity = 46/109 (42.20%), Postives = 72/109 (66.06%), Query Frame = 2 Query: 2 QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMNEINEENKILQNNYS--QSKLNQSEIDKINLQKENEKLKSSNNHAE-----CKLKFNQS 307 QLKM++SN SI+KLKL+L++S+++ K LQ+E NNIKSGFA+QIE+L+ + E + + LQ ++ + KL S+ + L+ ENE +K+ + E C+LK S Sbjct: 17 QLKMDNSNRSIKKLKLQLSQSENKCKSLQNE-NNIKSGFAAQIEQLQSALKESDRKMGSLQRDFGDCKEKLKHSQERNLQLENENETVKTKFHQFEEITKDCQLKMEDS 124 The following BLAST results are available for this feature:
BLAST of SMED30019210 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30019210 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30019210 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30019210 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30019210 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019210 ID=SMED30019210|Name=SMED30019210|organism=Schmidtea mediterranea sexual|type=transcript|length=487bpback to top protein sequence of SMED30019210-orf-1 >SMED30019210-orf-1 ID=SMED30019210-orf-1|Name=SMED30019210-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp QLKMEDSNSSIEKLKLELNRSKDEYKRLQDENNNIKSGFASQIEKLELKMback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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