BHLH domain-containing protein

Overview
NameBHLH domain-containing protein
Smed IDSMED30019204
Length (bp)742
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of BHLH domain-containing protein (SMED30019204) t-SNE clustered cells

Violin plots show distribution of expression levels for BHLH domain-containing protein (SMED30019204) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of BHLH domain-containing protein (SMED30019204) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for BHLH domain-containing protein (SMED30019204) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30019204

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30019204 DN306140ncbi_smed_estsPMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30019204 DN306140smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30019204 DN306140ncbi_smed_estsPMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30019204 DN306140smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30019204 DN306140ncbi_smed_estsPMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30019204 DN306140smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30019204 DN306140ncbi_smed_estsPMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30019204 DN306140smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Human
Match: ATOH8 (atonal bHLH transcription factor 8 [Source:HGNC Symbol;Acc:HGNC:24126])

HSP 1 Score: 68.5514 bits (166), Expect = 6.413e-13
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL++L + D + + S+  F++ V +   T+  E + K+
Sbjct:  244 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSADHSNLSFSECVQRCTRTLQAEGRAKK 318
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Fly
Match: net (gene:FBgn0002931 transcript:FBtr0290323)

HSP 1 Score: 72.0182 bits (175), Expect = 2.782e-14
Identity = 34/79 (43.04%), Postives = 51/79 (64.56%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSE 679
            HTIS+A+E+LRQ VP+Y ++ KLSKL +LR+AC YI +LS++  ED + + S       +  +  TI  E K+K+   E
Sbjct:  282 HTISAAYETLRQAVPAYASTQKLSKLSVLRVACSYILTLSRMAGEDYSADQSVPSIATCLEAVTSTIQTEGKVKRKKDE 360
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Fly
Match: net (gene:FBgn0002931 transcript:FBtr0330637)

HSP 1 Score: 72.0182 bits (175), Expect = 2.782e-14
Identity = 34/79 (43.04%), Postives = 51/79 (64.56%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSE 679
            HTIS+A+E+LRQ VP+Y ++ KLSKL +LR+AC YI +LS++  ED + + S       +  +  TI  E K+K+   E
Sbjct:  282 HTISAAYETLRQAVPAYASTQKLSKLSVLRVACSYILTLSRMAGEDYSADQSVPSIATCLEAVTSTIQTEGKVKRKKDE 360
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Zebrafish
Match: atoh8 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:561606])

HSP 1 Score: 65.855 bits (159), Expect = 2.346e-12
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   +  +  F + V +   T+  E + K+
Sbjct:  189 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHRNMSFRECVEQCTRTLQAEGRSKK 263
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Zebrafish
Match: atoh8 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:561606])

HSP 1 Score: 65.855 bits (159), Expect = 2.348e-12
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   +  +  F + V +   T+  E + K+
Sbjct:  189 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHRNMSFRECVEQCTRTLQAEGRSKK 263
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Xenopus
Match: ATOH8 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:100490889])

HSP 1 Score: 67.3958 bits (163), Expect = 7.072e-13
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL++L + D +V+ S+  F + V +   T+  E + K+
Sbjct:  169 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSVDHSNLSFPECVEQCTRTLQSEGRSKK 243
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Mouse
Match: Atoh8 (atonal bHLH transcription factor 8 [Source:MGI Symbol;Acc:MGI:1918343])

HSP 1 Score: 68.9366 bits (167), Expect = 3.724e-13
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL++L + D + + S+  F++ V +   T+  E + K+
Sbjct:  245 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSADHSNLSFSECVQRCTRTLQAEGRAKK 319
[Close]
BLAST of BHLH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q99NA2|ATOH8_MOUSE (Protein atonal homolog 8 OS=Mus musculus OX=10090 GN=Atoh8 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.605e-12
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL++L + D + + S+  F++ V +   T+  E + K+
Sbjct:  245 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSADHSNLSFSECVQRCTRTLQAEGRAKK 319
[Close]
BLAST of BHLH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q96SQ7|ATOH8_HUMAN (Protein atonal homolog 8 OS=Homo sapiens OX=9606 GN=ATOH8 PE=2 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 3.079e-12
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL++L + D + + S+  F++ V +   T+  E + K+
Sbjct:  244 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSADHSNLSFSECVQRCTRTLQAEGRAKK 318
[Close]
BLAST of BHLH domain-containing protein vs. UniProt/SwissProt
Match: sp|D2CLZ9|ATOH8_DANRE (Protein atonal homolog 8 OS=Danio rerio OX=7955 GN=atoh8 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.773e-11
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   +  +  F + V +   T+  E + K+
Sbjct:  189 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHRNMSFRECVEQCTRTLQAEGRSKK 263
[Close]
BLAST of BHLH domain-containing protein vs. TrEMBL
Match: A0A2R7VTR1 (BHLH domain-containing protein OS=Oncopeltus fasciatus OX=7536 GN=OFAS_OFAS001232 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.389e-13
Identity = 37/75 (49.33%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP+Y ++ KLSKL +LRIA  YI +LS++I ED +V+ S     + V+K++ TI  E K+K+
Sbjct:  101 HTISAAFETLRRAVPAYSHNQKLSKLSVLRIAASYILTLSRVIGEDYSVDQSEPSIEECVDKVSNTIQMEGKLKK 175
[Close]
BLAST of BHLH domain-containing protein vs. TrEMBL
Match: A0A0L8HXY6 (BHLH domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22003818mg PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.109e-12
Identity = 38/75 (50.67%), Postives = 53/75 (70.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VPSY  + KLSKL ILRIACCYI +L++L N D + E ++  F+D V+    TI  E + ++
Sbjct:  626 HTISAAFENLRRAVPSYSCNQKLSKLAILRIACCYILALARLANMDYSAEQTAMTFSDCVDLCTRTIQTEGRARR 700
[Close]
BLAST of BHLH domain-containing protein vs. TrEMBL
Match: A0A182GBF5 (BHLH domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG006926 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.219e-12
Identity = 36/79 (45.57%), Postives = 53/79 (67.09%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSE 679
            HTIS+A+E LRQ VP+Y ++ KLSKL ILRIAC YI +LS++  +D + ++S    +D +  +  TI  E K+K+   E
Sbjct:  253 HTISAAYEKLRQAVPAYSDAQKLSKLSILRIACSYILTLSRMAGDDYSADASEPSIDDCIEMVTKTIQTEGKIKKKKDE 331
[Close]
BLAST of BHLH domain-containing protein vs. TrEMBL
Match: A0A1J1I0M8 (CLUMA_CG007430, isoform A OS=Clunio marinus OX=568069 GN=CLUMA_CG007430 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.313e-12
Identity = 34/79 (43.04%), Postives = 54/79 (68.35%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSE 679
            HTIS+A+++LR+ +PSY N+ KLSKL +LR+AC YI +LS++  ED +V+ S     D ++ +  TI  E K+++   E
Sbjct:  255 HTISAAYDTLRKAIPSYSNTQKLSKLSVLRVACSYILTLSRMTGEDFSVDQSEPPVQDCIDSVTRTIQTEGKLRRKKDE 333
[Close]
BLAST of BHLH domain-containing protein vs. TrEMBL
Match: R7U7G9 (BHLH domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_157958 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.329e-12
Identity = 38/75 (50.67%), Postives = 49/75 (65.33%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFESLR+ VPSY  + +LSKL ILRIA  YI +LS+L + D + + S   F D V+    TI  E K K+
Sbjct:   18 HTISAAFESLRRAVPSYSYNQRLSKLAILRIAGSYITALSRLADLDYSADQSEPTFADCVDTCTRTIQAEGKAKR 92
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000042212.1 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:103039142])

HSP 1 Score: 67.781 bits (164), Expect = 3.574e-13
Identity = 36/75 (48.00%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   + S+  F + V +   T+  E + K+
Sbjct:  186 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHSNMSFRECVEQCTRTLQAEGRSKK 260
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000052339.1 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:103039142])

HSP 1 Score: 67.781 bits (164), Expect = 3.574e-13
Identity = 36/75 (48.00%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   + S+  F + V +   T+  E + K+
Sbjct:  186 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHSNMSFRECVEQCTRTLQAEGRSKK 260
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000053423.1 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:103039142])

HSP 1 Score: 67.781 bits (164), Expect = 3.574e-13
Identity = 36/75 (48.00%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   + S+  F + V +   T+  E + K+
Sbjct:  186 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHSNMSFRECVEQCTRTLQAEGRSKK 260
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000052689.1 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:103039142])

HSP 1 Score: 67.781 bits (164), Expect = 3.574e-13
Identity = 36/75 (48.00%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL + D   + S+  F + V +   T+  E + K+
Sbjct:  186 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLADLDYTPDHSNMSFRECVEQCTRTLQAEGRSKK 260
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Nematostella
Match: EDO36266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ30])

HSP 1 Score: 61.2326 bits (147), Expect = 1.812e-12
Identity = 32/75 (42.67%), Postives = 44/75 (58.67%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HT+S AFESLR+ VPSY    KLSKL ILR+A  YI +L+ L   D + + S   F + V +    +  E + K+
Sbjct:   16 HTMSEAFESLRKAVPSYSQDQKLSKLAILRLATSYISALADLTESDTSAK-SLKHFAECVEQCTRALQAEGRAKR 89
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000044230.1 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:101168275])

HSP 1 Score: 65.4698 bits (158), Expect = 4.299e-12
Identity = 35/75 (46.67%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL   +++  S    F + V++   T+  E + K+
Sbjct:  283 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLA--ELHPGSDRSGFTECVDQCTRTLQAEGRSKK 355
[Close]
BLAST of BHLH domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000027875.1 (atonal bHLH transcription factor 8 [Source:NCBI gene;Acc:101168275])

HSP 1 Score: 65.4698 bits (158), Expect = 4.993e-12
Identity = 35/75 (46.67%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQ 667
            HTIS+AFE+LR+ VP Y    KLSKL ILRIAC YI SL+QL   +++  S    F + V++   T+  E + K+
Sbjct:  283 HTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLAQLA--ELHPGSDRSGFTECVDQCTRTLQAEGRSKK 355
[Close]
BLAST of BHLH domain-containing protein vs. Planmine SMEST
Match: SMESG000018830.1 (SMESG000018830.1)

HSP 1 Score: 291.967 bits (746), Expect = 2.759e-101
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 2 
Query:  173 MMITNDYLNVYNFNNISWMILNNPSLPLEQNPKPLDFSDVKSTSQSDFSETASFVSEKTENRSNIICXXXXXXXXXXXXXXXXXXXXXXXHTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSEK 682
            MMITNDYLNVYNFNNISWMILNNPSLPLEQNPKPLDFSDVKSTSQSDFSETASFVSEKTENRSNIICVKKRVKVRQVRRMEANARERNRVHTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSEK
Sbjct:    1 MMITNDYLNVYNFNNISWMILNNPSLPLEQNPKPLDFSDVKSTSQSDFSETASFVSEKTENRSNIICVKKRVKVRQVRRMEANARERNRVHTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYVNKLAITIAQESKMKQFLSEK 170
[Close]
BLAST of BHLH domain-containing protein vs. Planmine SMEST
Match: SMESG000056587.1 (SMESG000056587.1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.945e-21
Identity = 46/75 (61.33%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:  443 HTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESS-SDQFNDYVNKLAITIAQESKMK 664
            HTIS+AFE+LR  VP Y NS KLSKLGILRIACCYIESL+QL+     V+   +  F+  + KLA TIA ESK+K
Sbjct:  162 HTISAAFETLRHAVPGYSNSPKLSKLGILRIACCYIESLNQLLQTSDKVDPQFNSNFSKCIQKLAQTIASESKLK 236
The following BLAST results are available for this feature:
BLAST of BHLH domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ATOH86.413e-1346.67atonal bHLH transcription factor 8 [Source:HGNC Sy... [more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
net2.782e-1443.04gene:FBgn0002931 transcript:FBtr0290323[more]
net2.782e-1443.04gene:FBgn0002931 transcript:FBtr0330637[more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
atoh82.346e-1246.67atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
atoh82.348e-1246.67atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ATOH87.072e-1348.00atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Atoh83.724e-1346.67atonal bHLH transcription factor 8 [Source:MGI Sym... [more]
back to top
BLAST of BHLH domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q99NA2|ATOH8_MOUSE2.605e-1246.67Protein atonal homolog 8 OS=Mus musculus OX=10090 ... [more]
sp|Q96SQ7|ATOH8_HUMAN3.079e-1246.67Protein atonal homolog 8 OS=Homo sapiens OX=9606 G... [more]
sp|D2CLZ9|ATOH8_DANRE1.773e-1146.67Protein atonal homolog 8 OS=Danio rerio OX=7955 GN... [more]
back to top
BLAST of BHLH domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2R7VTR11.389e-1349.33BHLH domain-containing protein OS=Oncopeltus fasci... [more]
A0A0L8HXY61.109e-1250.67BHLH domain-containing protein OS=Octopus bimaculo... [more]
A0A182GBF51.219e-1245.57BHLH domain-containing protein OS=Aedes albopictus... [more]
A0A1J1I0M81.313e-1243.04CLUMA_CG007430, isoform A OS=Clunio marinus OX=568... [more]
R7U7G91.329e-1250.67BHLH domain-containing protein OS=Capitella teleta... [more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSAMXT00000042212.13.574e-1348.00atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
ENSAMXT00000052339.13.574e-1348.00atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
ENSAMXT00000053423.13.574e-1348.00atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
ENSAMXT00000052689.13.574e-1348.00atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO362661.812e-1242.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of BHLH domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000044230.14.299e-1246.67atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
ENSORLT00000027875.14.993e-1246.67atonal bHLH transcription factor 8 [Source:NCBI ge... [more]
back to top
BLAST of BHLH domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000018830.12.759e-101100.00SMESG000018830.1[more]
SMESG000056587.12.945e-2161.33SMESG000056587.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019204 ID=SMED30019204|Name=BHLH domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=742bp
CACCAAAAACAACTATGAGGAACAGGCACACACGCATCAGTGCAATCTGT
CGGTACTCGACATCTGTTATCATTTTTGGCTTCAAATTTACTGAATTGAT
TTTAATTGGTCAGAATTTATTCTATATCTCTATATAATAAATTGTTTAGA
GGATTTATATTTTCGAATCGCAATGATGATTACTAATGATTATTTGAATG
TTTATAATTTCAACAATATTTCTTGGATGATTCTCAACAATCCTTCTTTA
CCACTTGAACAAAATCCAAAGCCATTAGATTTTAGTGATGTCAAATCTAC
TTCACAATCTGATTTTTCGGAAACTGCAAGTTTTGTCAGCGAAAAAACTG
AAAACAGATCAAATATTATCTGTGTGAAAAAACGAGTAAAAGTTCGACAA
GTTCGCAGAATGGAAGCCAATGCCAGAGAAAGAAATCGTGTTCATACAAT
CAGTTCAGCATTCGAAAGTCTTCGACAAGTAGTACCGTCATATTACAATT
CATATAAACTTTCTAAATTGGGAATCTTGAGAATAGCTTGCTGCTACATT
GAATCATTAAGTCAGCTAATTAATGAAGATGTTAACGTCGAAAGTTCTTC
GGACCAATTCAATGATTACGTGAACAAACTTGCGATAACCATTGCCCAGG
AAAGCAAAATGAAACAATTCTTATCAGAAAAATAAATAAAATTTTAATAA
AATTATTTAACGATGGCTGTGATAATATTTTGTAAATATATA
back to top

protein sequence of SMED30019204-orf-1

>SMED30019204-orf-1 ID=SMED30019204-orf-1|Name=SMED30019204-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=171bp
MMITNDYLNVYNFNNISWMILNNPSLPLEQNPKPLDFSDVKSTSQSDFSE
TASFVSEKTENRSNIICVKKRVKVRQVRRMEANARERNRVHTISSAFESL
RQVVPSYYNSYKLSKLGILRIACCYIESLSQLINEDVNVESSSDQFNDYV
NKLAITIAQESKMKQFLSEK*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR032660ATOH8
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO:0030154cell differentiation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 83..135
e-value: 8.3E-14
score: 61.9
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 78..129
e-value: 1.3E-15
score: 57.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 77..129
score: 15.04
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 75..133
e-value: 3.41367E-14
score: 61.8463
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 73..140
e-value: 5.2E-21
score: 75.9
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 76..137
IPR032660Protein atonal homologue 8PANTHERPTHR19290:SF102PROTEIN ATONAL HOMOLOG 8coord: 37..164
NoneNo IPR availablePANTHERPTHR19290BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATEDcoord: 37..164