Glyco_hydro_59M domain-containing protein

Overview
NameGlyco_hydro_59M domain-containing protein
Smed IDSMED30019176
Length (bp)2333
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glyco_hydro_59M domain-containing protein (SMED30019176) t-SNE clustered cells

Violin plots show distribution of expression levels for Glyco_hydro_59M domain-containing protein (SMED30019176) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glyco_hydro_59M domain-containing protein (SMED30019176) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Glyco_hydro_59M domain-containing protein (SMED30019176) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019176

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30019176SMESG000068390.1 SMESG000042600.1 PL030008B10H05ncbi_smed_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30019176SMESG000068390.1 Contig1994GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
protonephridiaSMED30019176SMESG000068390.1 dd_Smed_v4_2699_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30019176SMESG000068390.1 dd_Smed_v4_2699_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019176SMESG000068390.1 dd_Smed_v4_2699_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019176SMESG000068390.1 dd_Smed_v4_2699_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Human
Match: GALC (galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:4115])

HSP 1 Score: 355.525 bits (911), Expect = 1.466e-122
Identity = 222/546 (40.66%), Postives = 320/546 (58.61%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIF-HFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGT-LESPTKK--ILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILS--VMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            W+FPGW+G     PY      A Y + W+   +  ++++  YIG+WNE+ Y+  YIK LR+ L+ +G   VKII +D  WE I++ ++ D+EL + +  IG HYPGT+S+  AKL+GK LWSSEDFST N + GAGCW RILNQNY+NG MTSTIAWNL+A+YY+ LPY RCGLMTA EPWSG+Y V + VW+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S CIRP LP F    Q  T     + +++  +  +W T L       +F   + +   DS G     L  D + TL+T++T +K  +P PP S PFPS Y DDF   YP  +E      Q G FE  T +E P +    L Q +  +PI+W  D    +  I++IG++N +++ +   V I  P       VF+  R+N  G     A G+FFW+ F +  + ++ D+    IY   R+ ++  +WY +T+ +K       ++++ +   T++    P +G+A +GT  F  A FD+F ++
Sbjct:  151 WSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTD-GLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEI-PELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA---SNTISIIGDYNWTNLTIKCDVYIETPD---TGGVFIAGRVNKGGILIRSARGIFFWI-FANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLW--TDIPVNFPKNGWAAIGTHSFEFAQFDNFLVE 682

HSP 2 Score: 106.301 bits (264), Expect = 1.466e-122
Identity = 62/86 (72.09%), Postives = 71/86 (82.56%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIGA+SGGGATS+LL  Y  PY+SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   54 EFDGIGAVSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKK 139
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Human
Match: GALC (galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:4115])

HSP 1 Score: 355.525 bits (911), Expect = 3.511e-122
Identity = 222/546 (40.66%), Postives = 320/546 (58.61%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIF-HFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGT-LESPTKK--ILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILS--VMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            W+FPGW+G     PY      A Y + W+   +  ++++  YIG+WNE+ Y+  YIK LR+ L+ +G   VKII +D  WE I++ ++ D+EL + +  IG HYPGT+S+  AKL+GK LWSSEDFST N + GAGCW RILNQNY+NG MTSTIAWNL+A+YY+ LPY RCGLMTA EPWSG+Y V + VW+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S CIRP LP F    Q  T     + +++  +  +W T L       +F   + +   DS G     L  D + TL+T++T +K  +P PP S PFPS Y DDF   YP  +E      Q G FE  T +E P +    L Q +  +PI+W  D    +  I++IG++N +++ +   V I  P       VF+  R+N  G     A G+FFW+ F +  + ++ D+    IY   R+ ++  +WY +T+ +K       ++++ +   T++    P +G+A +GT  F  A FD+F ++
Sbjct:  125 WSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTD-GLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEI-PELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA---SNTISIIGDYNWTNLTIKCDVYIETPD---TGGVFIAGRVNKGGILIRSARGIFFWI-FANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLW--TDIPVNFPKNGWAAIGTHSFEFAQFDNFLVE 656

HSP 2 Score: 105.145 bits (261), Expect = 3.511e-122
Identity = 49/71 (69.01%), Postives = 56/71 (78.87%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +LL  Y  PY+SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   43 RLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKK 113
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Human
Match: GALC (galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:4115])

HSP 1 Score: 346.665 bits (888), Expect = 1.483e-119
Identity = 211/499 (42.28%), Postives = 296/499 (59.32%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIF-HFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGT-LESPTKK--ILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILS--VMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILK 1848
            W+FPGW+G     PY      A Y + W+   +  ++++  YIG+WNE+ Y+  YIK LR+ L+ +G   VKII +D  WE I++ ++ D+EL + +  IG HYPGT+S+  AKL+GK LWSSEDFST N + GAGCW RILNQNY+NG MTSTIAWNL+A+YY+ LPY RCGLMTA EPWSG+Y V + VW+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S CIRP LP F    Q  T     + +++  +  +W T L       +F   + +   DS G     L  D + TL+T++T +K  +P PP S PFPS Y DDF   YP  +E      Q G FE  T +E P +    L Q +  +PI+W  D    +  I++IG++N +++ +   V I  P       VF+  R+N  G     A G+FFW+ F +  + ++ D+    IY   R+ ++  +WY +T+ +K
Sbjct:   95 WSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTD-GLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEI-PELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA---SNTISIIGDYNWTNLTIKCDVYIETPD---TGGVFIAGRVNKGGILIRSARGIFFWI-FANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIK 581

HSP 2 Score: 105.145 bits (261), Expect = 1.483e-119
Identity = 49/71 (69.01%), Postives = 56/71 (78.87%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +LL  Y  PY+SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   13 RLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKK 83
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Human
Match: GALC (galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:4115])

HSP 1 Score: 355.525 bits (911), Expect = 1.616e-113
Identity = 219/544 (40.26%), Postives = 320/544 (58.82%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIF-HFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGT-LESPTKK--ILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            W+FPGW+G     PY      A Y + W+   +  ++++  YIG+WNE+ Y+  YIK LR+ L+ +G   VKII +D  WE I++ ++ D+EL + +  IG HYPGT+S+  AKL+GK LWSSEDFST N + GAGCW RILNQNY+NG MTSTIAWNL+A+YY+ LPY RCGLMTA EPWSG+Y V + VW+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S CIRP LP F    Q  T     + +++  +  +W T L       +F   + +   DS G     L  D + TL+T++T +K  +P PP S PFPS Y DDF   YP  +E      Q G FE  T +E P +    L Q +  +PI+W  D    +  I++IG++N +++ +   + + +      VF+  R+N  G     A G+FFW+ F +  + ++ D+    IY   R+ ++  +WY +T+ +K       ++++ +   T++    P +G+A +GT  F  A FD+F ++
Sbjct:  128 WSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTDG-LGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEI-PELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA---SNTISIIGDYNWTNLTIKCDVYIET-PDTGGVFIAGRVNKGGILIRSARGIFFWI-FANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLW--TDIPVNFPKNGWAAIGTHSFEFAQFDNFLVE 659

HSP 2 Score: 75.8702 bits (185), Expect = 1.616e-113
Identity = 35/51 (68.63%), Postives = 40/51 (78.43%), Query Frame = 2 
Query:  221 PQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            P FGASLH+LKVEIGGD Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   66 PNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKK 116
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Human
Match: GALC (galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:4115])

HSP 1 Score: 258.455 bits (659), Expect = 2.353e-93
Identity = 131/241 (54.36%), Postives = 170/241 (70.54%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENM 1095
            W+FPGW+G     PY      A Y + W+   +  ++++  YIG+WNE+ Y+  YIK LR+ L+ +G   VKII +D  WE I++ ++ D+EL + +  IG HYPGT+S+  AKL+GK LWSSEDFST N + GAGCW RILNQNY+NG MTSTIAWNL+A+YY+ LPY RCGLMTA EPWSG+Y V + VW+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E M
Sbjct:  148 WSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTDG-LGNLTIIIETM 384

HSP 2 Score: 105.916 bits (263), Expect = 2.353e-93
Identity = 62/86 (72.09%), Postives = 71/86 (82.56%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIGA+SGGGATS+LL  Y  PY+SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   51 EFDGIGAVSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKK 136
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Celegans
Match: C29E4.10 (Putative galactocerebrosidase [Source:UniProtKB/Swiss-Prot;Acc:Q95QT2])

HSP 1 Score: 210.305 bits (534), Expect = 8.862e-65
Identity = 160/498 (32.13%), Postives = 247/498 (49.60%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKII-GADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTN-DPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLST--ISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFL--AGGVFFWLNFTSSEFLISTDINSEKIYDQ--RRINLSYNQWYNMTIIL 1845
            WAFPGW+GN    PY      A Y +NWLK GR+ +N +   +G+WNE+ +   Y+K LR+ L+  GF    I+ G   + + +   + D         IG HYPG    +  + SGK +W+SED+ST N + G GC AR +N N++NGN+T  I+W+L++A+Y  LP+ RCGL    E     +       +  + T   + GWK   + + SG FDGGG+YVT  +   K+ TI +E MS+  S+C   +  P+  K   + ++F  +    F+       ++S N  PSK F          +  I  +LPV+    L+T  +ST K+        +   P  Y DDF++Y  + EP   +PQ GS+ V    +      VQ V    ISWC    I T P AV+  + K + IL+  + +P      S+ LG R N SGC   +    G+F  + F++ +  I +D     I  +   R  + + ++Y  +I L
Sbjct:  128 WAFPGWIGN---TPYDNVTETAEYVVNWLKIGRDTWNFDTFCVGVWNERNFSESYVKELRKILNLNGFNETLIVAGEGFRMDDSYSRLLDKRFINEYDIIGVHYPGGRIPENVQKSGKVIWASEDYSTDNRDTGEGCMARTMNWNFINGNITGMISWHLMSAFYPQLPWYRCGLARIDE---NKFQTEKAFHVLKYITSHVKRGWKI--LRESSGRFDGGGTYVTYTNG--KDSTIFVETMSYKKSLCEYSSPRPYHVKPSQL-IRFKFSEIPSFQGL-----NMSLNFGPSKFF------SKSVNSTITILLPVNSFGILTTLPVSTPKR----VTIKTPLVPLNYHDDFENYEFDEEPKFWMPQKGSWVVRNGRA------VQKVTRPSISWCTSH-IRT-PYAVMA-YRKKNSILNAEVNIPKHSTAKSIILGIRSNCSGCDIEVINCRGIFVEIEFSNKKVTIFSDFVDRSIIAEFKARRKVEFGKFYKFSIHL 590

HSP 2 Score: 57.7658 bits (138), Expect = 8.862e-65
Identity = 54/116 (46.55%), Postives = 75/116 (64.66%), Query Frame = 2 
Query:   41 LICLKLINANIISVTHSID--NLIYYPKFDXXXXXXXXXXXXKLLQTY--KNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKKL 376
             I + LI  +I + T++ID  +      FDG GA+SGGGATSKLL TY  +   + Q+L+ LF    G  L LLKVE+GGD QSTEGTE+SH   + +++ N  YE+QLI E +++
Sbjct:    5 FIKIILILPSIATKTNNIDIKSWRSAQTFDGFGAVSGGGATSKLLFTYFSEKSDRKQVLESLFNKDNG--LQLLKVEMGGDDQSTEGTESSHESEKGKISKN-NYEFQLISEVREI 117
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Zebrafish
Match: galcb (galactosylceramidase b [Source:ZFIN;Acc:ZDB-GENE-040426-2115])

HSP 1 Score: 356.295 bits (913), Expect = 2.860e-124
Identity = 221/546 (40.48%), Postives = 304/546 (55.68%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQT--DLSTNDPSKIFHFEKVIQPDS--SGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGT-LESPTKKI--LVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGWVGN    PY  P+  A+Y + W+   +  ++++  YIG+WNEK ++V YIK LR  LDK G   VKII +D  WE I S +  D EL   I  +G HYPGT +   A  +GK LWSSED+ST N   G GCWARILNQNYVNG MT+TI+WNL+A+YY++LP+ R G+MTA EPWSGNY V + VW T+HTTQFT+PGW Y       GHF  GGSYV L D +  NL+II E M+HD SVCIRP L P+    QN+T      T     Q  +W +  D  T+   K   F+K + P     G     L VD + T +TI+  ++  +P PP S PFP  Y DDF      +E      Q G FE  T L  P   +  L Q V  +PI+W  D     + I+VIG++   D+++S  + + +++    VF+ +R++  G       GVFFW+ +    + I+ D+    +  +         WY +T  L+ +  +   D        N    +P+HG+  +GT  F LA FD+F ++
Sbjct:  133 WAFPGWVGNGKNWPYDFPDITASYVVAWIHGAKKYHDLDIDYIGIWNEKSFNVKYIKLLRYLLDKRGLEKVKIIASDDLWEPITSAVFADKELYDAIEVLGVHYPGTKTVPRALKTGKKLWSSEDYSTFNDNVGGGCWARILNQNYVNGKMTATISWNLVASYYENLPFGRDGIMTANEPWSGNYVVESPVWTTAHTTQFTEPGWTYLQT---VGHFTHGGSYVALTD-QRGNLSIITETMTHDHSVCIRPPLRPYNVTVQNVTFNL-KGTFAFISQLDVWHSKFDFKTD---KTVLFQK-LDPVKVIGGSFSIELDVDEIYTFTTITNGQRGTYPDPPPSAPFPKSYRDDFNVSGNFSEAPNFADQTGVFEYFTNLTDPGPHVFTLRQVVTQRPITWAADAD---QTISVIGDYAWHDVMVSCDVYMEAVQ-TGGVFIAARVDRGGGVIRSTRGVFFWV-YADGTYKITNDLRGLAVLAKGSAGTRARTWYTLT--LRVQGNYASADLNGYSLWKNAVLLNPLHGWVAIGTSSFQLAQFDNFVIE 662

HSP 2 Score: 110.538 bits (275), Expect = 2.860e-124
Identity = 71/121 (58.68%), Postives = 85/121 (70.25%), Query Frame = 2 
Query:   11 MMISNYCLVFLICLKLINANIISVTHSIDNLIYYPKFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            M I N   VFL     I+  +       D+L     FDGIG +SGGGATS+LL  Y+ PY+SQILDYLFKP+FGASL +LKVEIGGD+Q+T+GTE SH HYE + N  RGYEW L+VEAKK
Sbjct:    1 MYIGNQFCVFLSIFLYISGCLGENYDIDDSLGLGQTFDGIGGLSGGGATSRLLVNYEEPYQSQILDYLFKPKFGASLQILKVEIGGDAQTTDGTEPSHMHYEDDENYFRGYEWWLMVEAKK 121
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Zebrafish
Match: galca (galactosylceramidase a [Source:NCBI gene;Acc:449649])

HSP 1 Score: 360.533 bits (924), Expect = 6.251e-124
Identity = 222/544 (40.81%), Postives = 313/544 (57.54%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQP--DSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEV---GTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEV-TNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            WAFPGWVG  T  PY  PN  ANY I W+   +  +N++  YIG+WNEK +D  YIK LR  LD+ GF N+ II AD  W IAS ++ D  L+  +  IG HYPGTN+   A L+ + LWSSED+ST N + GAGCWARILNQNYVNG MTSTI+WN+IA+YY++L + R GLMTA EPWSG+Y V + +W+T+HTTQFTQPGW Y    +  G  + GGSYV L D    NLTII+E M+H+ S CIRP LP F    Q  T +   + A L     +W + L     +      K ++P    +G +   L VD V T++T++TA++  +P PP S PFP  Y+DDF    P  +E      Q G FE     T  S     L Q V ++P++W  D     + I++IG+++ SD+ +S  + + + +    VFL +R++  G +   A GVF+W+ + +  + ++ DI  +++  +         WY +T+ +K   G+F         +  N     P  G+A LGT  F  A FD+F +
Sbjct:  129 WAFPGWVGYGTQWPYFFPNVTANYVITWVMGAKQHHNLDIDYIGIWNEKAFDPTYIKVLRDALDRAGFTNIGIIAADGDWSIASAMLDDPYLNDAVEVIGVHYPGTNTVKEALLTERKLWSSEDYSTYNDDIGAGCWARILNQNYVNGKMTSTISWNVIASYYENLSFGRDGLMTAEEPWSGHYIVESPIWMTAHTTQFTQPGWFY---LQTVGKLNHGGSYVALTD-RKGNLTIIIETMTHEHSQCIRPPLPHFDVSPQIATFELKGSFAHL-ADLQVWYSKLDFKSGNGTLF--KQLRPIRAHNGLLSLKLDVDEVFTITTVTTAQRGFYPEPPKSCPFPKNYTDDFTIDNPPFSEAPYFADQTGVFEYFRNTTDNSSHAFTLRQVVTERPVAWAKDA---DQTISIIGDYSWSDVNVSCDVFIETPK-TGGVFLAARVDQGGESVRQAKGVFYWI-YANGTYRVTNDIGGKRVLAEGLSGTKAGVWYTLTLSVK---GYFATGSLNGFPLWKNAAVLEPKSGWAALGTLSFEYAQFDNFNV 657

HSP 2 Score: 105.145 bits (261), Expect = 6.251e-124
Identity = 61/86 (70.93%), Postives = 72/86 (83.72%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIG +SGGGATS+LL  Y+ PY+SQILDYLFKP FGASLH+LKVEIGGD+Q+T+GTE SH H + E N  RGYEW L+ EAKK
Sbjct:   32 EFDGIGGLSGGGATSRLLVNYEEPYRSQILDYLFKPNFGASLHILKVEIGGDAQTTDGTEPSHMHSKDEGNFFRGYEWWLMKEAKK 117
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Xenopus
Match: vdac2 (voltage-dependent anion channel 2 [Source:Xenbase;Acc:XB-GENE-954839])

HSP 1 Score: 369.392 bits (947), Expect = 4.665e-125
Identity = 222/543 (40.88%), Postives = 320/543 (58.93%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+GN    PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLD+ G  NV II AD  WEIA D++ D  L+  +  IG HYPGT+++  A L+GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    K  GH D GGSYV L D  + NLTI++E MSH++S+CIRP LP +    Q  T     +   L ++  +W + L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + +      VF+ +R++  G  T  A G+FFW+ F    + ++ D+  + I  +    +    W+ +T+ +   N +  ++   + +   +    P++G+A +GT  F  A FD+F ++
Sbjct:  143 WAFPGWIGNGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKVLRHTLDRLGLTNVGIITADGDWEIAHDVLVDPYLNEAVQVIGAHYPGTHAAQDAILTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LKTVGHLDKGGSYVALTD-RLGNLTIVIETMSHNNSICIRPPLPDYNVSAQYATFYLQGSFKNL-QELQVWYSSLDINN-TKPVTFKKLSPLKVQNGSFTLQLGVNEVYTITTLLTGQKGSFPDPPESKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNVTVTSDIYIET-PDTGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLMGQTILCKGLAGVRARSWHTLTLHIDGTNAFGLLNGNPLWK--EVVTGGPLNGWAAIGTSCFEFAQFDNFMIE 672

HSP 2 Score: 100.908 bits (250), Expect = 4.665e-125
Identity = 65/114 (57.02%), Postives = 83/114 (72.81%), Query Frame = 2 
Query:   35 VFLICLKLINANIISVTHSIDNLIYYPK-FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +F++ L L+        +S+D+L    + FDGIGAISGGGATS+LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   18 MFILWLVLLLFPGAPAQYSVDDLGGLGRMFDGIGAISGGGATSRLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 131
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Xenopus
Match: vdac2 (voltage-dependent anion channel 2 [Source:Xenbase;Acc:XB-GENE-954839])

HSP 1 Score: 360.918 bits (925), Expect = 1.987e-122
Identity = 221/544 (40.62%), Postives = 321/544 (59.01%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+GN    PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLDK    +VKII +D  W+ IA  +++D EL R +  IG HYPGT+++  A L+GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    K  GH D GGSYV L D  + NLTI++E MSH++S+CIRP LP +    Q  T     +   L ++  +W + L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + +      VF+ +R++  G  T  A G+FFW+ F    + ++ D+  + I  +    +    W+ +T+ +   N +  ++   + +   +    P++G+A +GT  F  A FD+F ++
Sbjct:  143 WAFPGWIGNGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKLLRYTLDKNNLEHVKIIASDNLWQPIAFHMLQDPELLRVVDVIGAHYPGTHAAQDAILTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LKTVGHLDKGGSYVALTD-RLGNLTIVIETMSHNNSICIRPPLPDYNVSAQYATFYLQGSFKNL-QELQVWYSSLDINN-TKPVTFKKLSPLKVQNGSFTLQLGVNEVYTITTLLTGQKGSFPDPPESKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNVTVTSDIYIET-PDTGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLMGQTILCKGLAGVRARSWHTLTLHIDGTNAFGLLNGNPLWK--EVVTGGPLNGWAAIGTSCFEFAQFDNFMIE 673

HSP 2 Score: 100.908 bits (250), Expect = 1.987e-122
Identity = 65/114 (57.02%), Postives = 83/114 (72.81%), Query Frame = 2 
Query:   35 VFLICLKLINANIISVTHSIDNLIYYPK-FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +F++ L L+        +S+D+L    + FDGIGAISGGGATS+LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   18 MFILWLVLLLFPGAPAQYSVDDLGGLGRMFDGIGAISGGGATSRLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 131
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Xenopus
Match: vdac2 (voltage-dependent anion channel 2 [Source:Xenbase;Acc:XB-GENE-954839])

HSP 1 Score: 361.688 bits (927), Expect = 3.930e-122
Identity = 221/544 (40.62%), Postives = 321/544 (59.01%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+GN    PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLDK    +VKII +D  W+ IA  +++D EL R +  IG HYPGT+++  A L+GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    K  GH D GGSYV L D  + NLTI++E MSH++S+CIRP LP +    Q  T     +   L ++  +W + L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + +      VF+ +R++  G  T  A G+FFW+ F    + ++ D+  + I  +    +    W+ +T+ +   N +  ++   + +   +    P++G+A +GT  F  A FD+F ++
Sbjct:   92 WAFPGWIGNGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKLLRYTLDKNNLEHVKIIASDNLWQPIAFHMLQDPELLRVVDVIGAHYPGTHAAQDAILTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LKTVGHLDKGGSYVALTD-RLGNLTIVIETMSHNNSICIRPPLPDYNVSAQYATFYLQGSFKNL-QELQVWYSSLDINN-TKPVTFKKLSPLKVQNGSFTLQLGVNEVYTITTLLTGQKGSFPDPPESKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNVTVTSDIYIET-PDTGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLMGQTILCKGLAGVRARSWHTLTLHIDGTNAFGLLNGNPLWK--EVVTGGPLNGWAAIGTSCFEFAQFDNFMIE 622

HSP 2 Score: 98.9821 bits (245), Expect = 3.930e-122
Identity = 45/71 (63.38%), Postives = 55/71 (77.46%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   10 RLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 80
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Xenopus
Match: vdac2 (voltage-dependent anion channel 2 [Source:Xenbase;Acc:XB-GENE-954839])

HSP 1 Score: 361.688 bits (927), Expect = 4.798e-122
Identity = 221/544 (40.62%), Postives = 322/544 (59.19%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+GN    PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLDK    +VKII +D  W+ IA  +++D EL R +  IG HYPGT+++  A L+GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    K  GH D GGSYV L D  + NLTI++E MSH++S+CIRP LP +    Q  T  +   + K  ++  +W + L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + +      VF+ +R++  G  T  A G+FFW+ F    + ++ D+  + I  +    +    W+ +T+ +   N +  ++   + +   +    P++G+A +GT  F  A FD+F ++
Sbjct:  104 WAFPGWIGNGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKLLRYTLDKNNLEHVKIIASDNLWQPIAFHMLQDPELLRVVDVIGAHYPGTHAAQDAILTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LKTVGHLDKGGSYVALTD-RLGNLTIVIETMSHNNSICIRPPLPDYNVSAQYATF-YLQGSFKNLQELQVWYSSLDINN-TKPVTFKKLSPLKVQNGSFTLQLGVNEVYTITTLLTGQKGSFPDPPESKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNVTVTSDIYIET-PDTGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLMGQTILCKGLAGVRARSWHTLTLHIDGTNAFGLLNGNPLWK--EVVTGGPLNGWAAIGTSCFEFAQFDNFMIE 634

HSP 2 Score: 98.5969 bits (244), Expect = 4.798e-122
Identity = 45/71 (63.38%), Postives = 55/71 (77.46%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   22 RLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 92
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Xenopus
Match: vdac2 (voltage-dependent anion channel 2 [Source:Xenbase;Acc:XB-GENE-954839])

HSP 1 Score: 361.303 bits (926), Expect = 5.390e-122
Identity = 221/544 (40.62%), Postives = 322/544 (59.19%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+GN    PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLDK    +VKII +D  W+ IA  +++D EL R +  IG HYPGT+++  A L+GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    K  GH D GGSYV L D  + NLTI++E MSH++S+CIRP LP +    Q  T  +   + K  ++  +W + L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + +      VF+ +R++  G  T  A G+FFW+ F    + ++ D+  + I  +    +    W+ +T+ +   N +  ++   + +   +    P++G+A +GT  F  A FD+F ++
Sbjct:   98 WAFPGWIGNGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKLLRYTLDKNNLEHVKIIASDNLWQPIAFHMLQDPELLRVVDVIGAHYPGTHAAQDAILTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LKTVGHLDKGGSYVALTD-RLGNLTIVIETMSHNNSICIRPPLPDYNVSAQYATF-YLQGSFKNLQELQVWYSSLDINN-TKPVTFKKLSPLKVQNGSFTLQLGVNEVYTITTLLTGQKGSFPDPPESKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNVTVTSDIYIET-PDTGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLMGQTILCKGLAGVRARSWHTLTLHIDGTNAFGLLNGNPLWK--EVVTGGPLNGWAAIGTSCFEFAQFDNFMIE 628

HSP 2 Score: 98.5969 bits (244), Expect = 5.390e-122
Identity = 45/71 (63.38%), Postives = 55/71 (77.46%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   16 RLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 86
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Mouse
Match: Galc (galactosylceramidase [Source:MGI Symbol;Acc:MGI:95636])

HSP 1 Score: 373.244 bits (957), Expect = 2.634e-128
Identity = 219/545 (40.18%), Postives = 324/545 (59.45%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQ---PDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFE--VGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            W+FPGW+G     PY      A Y + W+   ++ ++++  YIG+WNE+ +D  YIK LR+ LD +G   V+II +D  WE I+S ++ D EL + +  IG HYPGT +   AK+SGK LWSSEDFST N   GAGCW+RILNQNY+NGNMTSTIAWNL+A+YY++LPY R GLMTA EPWSG+Y V + +W+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S+CIRP LP +    Q  T     +  ++ ++  +W T L T  P +  HF+++      D SG     L  D + TL+T++T +K  +PPPP S PFP+ Y DDF   YP+ +E      Q G FE  +   +   +  L Q +  +PI+W  D    +  I+VIG+ + ++M +   + + + R    VF+  R+N  G     A GVFFW+ F +  + ++ D+     Y     +++  +WY +T+ +K    +  ++  ++ +  N+  + P HG+A +GT  F  A FD+F+++
Sbjct:  151 WSFPGWLGKGFSWPYVNLQLTAYYVVRWILGAKHYHDLDIDYIGIWNERPFDANYIKELRKMLDYQGLQRVRIIASDNLWEPISSSLLLDQELWKVVDVIGAHYPGTYTVWNAKMSGKKLWSSEDFSTINSNVGAGCWSRILNQNYINGNMTSTIAWNLVASYYEELPYGRSGLMTAQEPWSGHYVVASPIWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTDG-LGNLTIIIETMSHQHSMCIRPYLPYYNVSHQLATFTLKGSLREI-QELQVWYTKLGT--PQQRLHFKQLDTLWLLDGSGSFTLELEEDEIFTLTTLTTGRKGSYPPPPSSKPFPTNYKDDFNVEYPLFSEAPNFADQTGVFEYYMNNEDREHRFTLRQVLNQRPITWAADA---SSTISVIGDHHWTNMTVQCDVYIETPRS-GGVFIAGRVNKGGILIRSATGVFFWI-FANGSYRVTADLGGWITYASGHADVTAKRWYTLTLGIKGYFAFGMLNGTILWK--NVRVKYPGHGWAAIGTHTFEFAQFDNFRVE 681

HSP 2 Score: 107.457 bits (267), Expect = 2.634e-128
Identity = 69/118 (58.47%), Postives = 83/118 (70.34%), Query Frame = 2 
Query:   20 SNYCLVFLICLKLINANIISVTHSIDNLIYYPKFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            S   +  L+C  L+      +  S D L    +FDGIGA+SGGGATS+LL  Y  PY+S+ILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HYE + N  RGYEW L+ EAKK
Sbjct:   25 SRVAVPLLLCALLVPGGAYVLDDS-DGLGR--EFDGIGAVSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYELDENYFRGYEWWLMKEAKK 139
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Mouse
Match: Galc (galactosylceramidase [Source:MGI Symbol;Acc:MGI:95636])

HSP 1 Score: 330.102 bits (845), Expect = 2.676e-104
Identity = 198/490 (40.41%), Postives = 292/490 (59.59%), Query Frame = 1 
Query:  541 IKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQ---PDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFE--VGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            ++ LR+ LD +G   V+II +D  WE I+S ++ D EL + +  IG HYPGT +   AK+SGK LWSSEDFST N   GAGCW+RILNQNY+NGNMTSTIAWNL+A+YY++LPY R GLMTA EPWSG+Y V + +W+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S+CIRP LP +    Q  T     +  ++ ++  +W T L T  P +  HF+++      D SG     L  D + TL+T++T +K  +PPPP S PFP+ Y DDF   YP+ +E      Q G FE  +   +   +  L Q +  +PI+W  D    +  I+VIG+ + ++M +   + + + R    VF+  R+N  G     A GVFFW+ F +  + ++ D+     Y     +++  +WY +T+ +K    +  ++  ++ +  N+  + P HG+A +GT  F  A FD+F+++
Sbjct:   13 LRELRKMLDYQGLQRVRIIASDNLWEPISSSLLLDQELWKVVDVIGAHYPGTYTVWNAKMSGKKLWSSEDFSTINSNVGAGCWSRILNQNYINGNMTSTIAWNLVASYYEELPYGRSGLMTAQEPWSGHYVVASPIWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTDG-LGNLTIIIETMSHQHSMCIRPYLPYYNVSHQLATFTLKGSLREI-QELQVWYTKLGT--PQQRLHFKQLDTLWLLDGSGSFTLELEEDEIFTLTTLTTGRKGSYPPPPSSKPFPTNYKDDFNVEYPLFSEAPNFADQTGVFEYYMNNEDREHRFTLRQVLNQRPITWAADA---SSTISVIGDHHWTNMTVQCDVYIETPRS-GGVFIAGRVNKGGILIRSATGVFFWI-FANGSYRVTADLGGWITYASGHADVTAKRWYTLTLGIKGYFAFGMLNGTILWK--NVRVKYPGHGWAAIGTHTFEFAQFDNFRVE 488
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. UniProt/SwissProt
Match: sp|P54818|GALC_MOUSE (Galactocerebrosidase OS=Mus musculus OX=10090 GN=Galc PE=1 SV=2)

HSP 1 Score: 373.244 bits (957), Expect = 1.602e-127
Identity = 219/545 (40.18%), Postives = 324/545 (59.45%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQ---PDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFE--VGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            W+FPGW+G     PY      A Y + W+   ++ ++++  YIG+WNE+ +D  YIK LR+ LD +G   V+II +D  WE I+S ++ D EL + +  IG HYPGT +   AK+SGK LWSSEDFST N   GAGCW+RILNQNY+NGNMTSTIAWNL+A+YY++LPY R GLMTA EPWSG+Y V + +W+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S+CIRP LP +    Q  T     +  ++ ++  +W T L T  P +  HF+++      D SG     L  D + TL+T++T +K  +PPPP S PFP+ Y DDF   YP+ +E      Q G FE  +   +   +  L Q +  +PI+W  D    +  I+VIG+ + ++M +   + + + R    VF+  R+N  G     A GVFFW+ F +  + ++ D+     Y     +++  +WY +T+ +K    +  ++  ++ +  N+  + P HG+A +GT  F  A FD+F+++
Sbjct:  151 WSFPGWLGKGFSWPYVNLQLTAYYVVRWILGAKHYHDLDIDYIGIWNERPFDANYIKELRKMLDYQGLQRVRIIASDNLWEPISSSLLLDQELWKVVDVIGAHYPGTYTVWNAKMSGKKLWSSEDFSTINSNVGAGCWSRILNQNYINGNMTSTIAWNLVASYYEELPYGRSGLMTAQEPWSGHYVVASPIWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTDG-LGNLTIIIETMSHQHSMCIRPYLPYYNVSHQLATFTLKGSLREI-QELQVWYTKLGT--PQQRLHFKQLDTLWLLDGSGSFTLELEEDEIFTLTTLTTGRKGSYPPPPSSKPFPTNYKDDFNVEYPLFSEAPNFADQTGVFEYYMNNEDREHRFTLRQVLNQRPITWAADA---SSTISVIGDHHWTNMTVQCDVYIETPRS-GGVFIAGRVNKGGILIRSATGVFFWI-FANGSYRVTADLGGWITYASGHADVTAKRWYTLTLGIKGYFAFGMLNGTILWK--NVRVKYPGHGWAAIGTHTFEFAQFDNFRVE 681

HSP 2 Score: 107.457 bits (267), Expect = 1.602e-127
Identity = 69/118 (58.47%), Postives = 83/118 (70.34%), Query Frame = 2 
Query:   20 SNYCLVFLICLKLINANIISVTHSIDNLIYYPKFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            S   +  L+C  L+      +  S D L    +FDGIGA+SGGGATS+LL  Y  PY+S+ILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HYE + N  RGYEW L+ EAKK
Sbjct:   25 SRVAVPLLLCALLVPGGAYVLDDS-DGLGR--EFDGIGAVSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYELDENYFRGYEWWLMKEAKK 139
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. UniProt/SwissProt
Match: sp|Q498K0|GALC_XENLA (Galactocerebrosidase OS=Xenopus laevis OX=8355 GN=galc PE=2 SV=2)

HSP 1 Score: 368.622 bits (945), Expect = 4.650e-126
Identity = 219/543 (40.33%), Postives = 318/543 (58.56%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+G     PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLD+ G  NV II AD  W IA D++ D  L+  +  IG HYPGT+++  A  +GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    +  GH D GGSYV L D  + NLT+I+E MSH++S+CIRP LP +    QN T     +   L ++  +W ++L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + ++     VF+ +R++  G  T  A G+FFW+ F    + ++ D+  E +  +    +    W+ +T+ +   N    ++   + +   +    P+HG+A +GT  F    FD+F ++
Sbjct:  143 WAFPGWIGYGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKVLRHTLDRLGLTNVGIIAADGDWGIAHDVLIDPYLNEAVQVIGAHYPGTHAAQDAIQTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LRTVGHLDKGGSYVALTD-RLGNLTVIIETMSHNNSICIRPPLPEYNVSAQNATFYLQGSFQNL-QELQVWYSNLDINN-TKPVTFKKLTPVKVQNGSFTLQLGVNEVYTVTTLLTGQKGSFPDPPKSKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNITVTCDIYIETVE-TGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLIGEIVLCKGLAGVRARSWHTLTLHVDGTNASGLLNGSPLWK--EVVTGGPLHGWAAIGTSSFEFTQFDNFMIE 672

HSP 2 Score: 107.071 bits (266), Expect = 4.650e-126
Identity = 66/114 (57.89%), Postives = 84/114 (73.68%), Query Frame = 2 
Query:   35 VFLICLKLINANIISVTHSIDNLIYYPK-FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +F++CL L+ A      +S+D+     + FDGIGAISGGGATS+LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   18 MFILCLALLLAPGAPAQYSVDDFGGLGRMFDGIGAISGGGATSRLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 131
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5SNX7|GALC_DANRE (Galactocerebrosidase OS=Danio rerio OX=7955 GN=galc PE=2 SV=1)

HSP 1 Score: 360.533 bits (924), Expect = 3.853e-123
Identity = 222/544 (40.81%), Postives = 313/544 (57.54%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQP--DSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEV---GTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEV-TNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            WAFPGWVG  T  PY  PN  ANY I W+   +  +N++  YIG+WNEK +D  YIK LR  LD+ GF N+ II AD  W IAS ++ D  L+  +  IG HYPGTN+   A L+ + LWSSED+ST N + GAGCWARILNQNYVNG MTSTI+WN+IA+YY++L + R GLMTA EPWSG+Y V + +W+T+HTTQFTQPGW Y    +  G  + GGSYV L D    NLTII+E M+H+ S CIRP LP F    Q  T +   + A L     +W + L     +      K ++P    +G +   L VD V T++T++TA++  +P PP S PFP  Y+DDF    P  +E      Q G FE     T  S     L Q V ++P++W  D     + I++IG+++ SD+ +S  + + + +    VFL +R++  G +   A GVF+W+ + +  + ++ DI  +++  +         WY +T+ +K   G+F         +  N     P  G+A LGT  F  A FD+F +
Sbjct:  129 WAFPGWVGYGTQWPYFFPNVTANYVITWVMGAKQHHNLDIDYIGIWNEKAFDPTYIKVLRDALDRAGFTNIGIIAADGDWSIASAMLDDPYLNDAVEVIGVHYPGTNTVKEALLTERKLWSSEDYSTYNDDIGAGCWARILNQNYVNGKMTSTISWNVIASYYENLSFGRDGLMTAEEPWSGHYIVESPIWMTAHTTQFTQPGWFY---LQTVGKLNHGGSYVALTD-RKGNLTIIIETMTHEHSQCIRPPLPHFDVSPQIATFELKGSFAHL-ADLQVWYSKLDFKSGNGTLF--KQLRPIRAHNGLLSLKLDVDEVFTITTVTTAQRGFYPEPPKSCPFPKNYTDDFTIDNPPFSEAPYFADQTGVFEYFRNTTDNSSHAFTLRQVVTERPVAWAKDA---DQTISIIGDYSWSDVNVSCDVFIETPK-TGGVFLAARVDQGGESVRQAKGVFYWI-YANGTYRVTNDIGGKRVLAEGLSGTKAGVWYTLTLSVK---GYFATGSLNGFPLWKNAAVLEPKSGWAALGTLSFEYAQFDNFNV 657

HSP 2 Score: 105.145 bits (261), Expect = 3.853e-123
Identity = 61/86 (70.93%), Postives = 72/86 (83.72%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIG +SGGGATS+LL  Y+ PY+SQILDYLFKP FGASLH+LKVEIGGD+Q+T+GTE SH H + E N  RGYEW L+ EAKK
Sbjct:   32 EFDGIGGLSGGGATSRLLVNYEEPYRSQILDYLFKPNFGASLHILKVEIGGDAQTTDGTEPSHMHSKDEGNFFRGYEWWLMKEAKK 117
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. UniProt/SwissProt
Match: sp|P54803|GALC_HUMAN (Galactocerebrosidase OS=Homo sapiens OX=9606 GN=GALC PE=1 SV=3)

HSP 1 Score: 355.525 bits (911), Expect = 6.457e-122
Identity = 222/546 (40.66%), Postives = 320/546 (58.61%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIF-HFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGT-LESPTKK--ILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILS--VMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            W+FPGW+G     PY      A Y + W+   +  ++++  YIG+WNE+ Y+  YIK LR+ L+ +G   VKII +D  WE I++ ++ D+EL + +  IG HYPGT+S+  AKL+GK LWSSEDFST N + GAGCW RILNQNY+NG MTSTIAWNL+A+YY+ LPY RCGLMTA EPWSG+Y V + VW+++HTTQFTQPGW Y       GH + GGSYV L D  + NLTII+E MSH  S CIRP LP F    Q  T     + +++  +  +W T L       +F   + +   DS G     L  D + TL+T++T +K  +P PP S PFPS Y DDF   YP  +E      Q G FE  T +E P +    L Q +  +PI+W  D    +  I++IG++N +++ +   V I  P       VF+  R+N  G     A G+FFW+ F +  + ++ D+    IY   R+ ++  +WY +T+ +K       ++++ +   T++    P +G+A +GT  F  A FD+F ++
Sbjct:  151 WSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLDAELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT---VGHLEKGGSYVALTD-GLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEI-PELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPITWAADA---SNTISIIGDYNWTNLTIKCDVYIETPD---TGGVFIAGRVNKGGILIRSARGIFFWI-FANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLW--TDIPVNFPKNGWAAIGTHSFEFAQFDNFLVE 682

HSP 2 Score: 106.301 bits (264), Expect = 6.457e-122
Identity = 62/86 (72.09%), Postives = 71/86 (82.56%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIGA+SGGGATS+LL  Y  PY+SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   54 EFDGIGAVSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKK 139
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. UniProt/SwissProt
Match: sp|Q0VA39|GALC_XENTR (Galactocerebrosidase OS=Xenopus tropicalis OX=8364 GN=galc PE=2 SV=1)

HSP 1 Score: 360.918 bits (925), Expect = 7.018e-122
Identity = 221/544 (40.62%), Postives = 321/544 (59.01%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVGTLES-PTKKILV--QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+GN    PY  P+  A Y ++W+   +  +N++  YIG+WNE+ YD+ YIK LR TLDK    +VKII +D  W+ IA  +++D EL R +  IG HYPGT+++  A L+GK LW+SED+ST N E G GCWARILNQNYVNGNMTSTI+WN++A+YY+ LP+   GLMTA EPWSGNY V T +WIT+HTTQFTQPGW Y    K  GH D GGSYV L D  + NLTI++E MSH++S+CIRP LP +    Q  T     +   L ++  +W + L  N+ +K   F+K+      +G     L V+ V T++T+ T +K   P PP S PFP +Y DDF    P  +E      Q G FE  T  S P   +    Q +  +PI+W  D     + I+VIGN+  S++ ++  I + +      VF+ +R++  G  T  A G+FFW+ F    + ++ D+  + I  +    +    W+ +T+ +   N +  ++   + +   +    P++G+A +GT  F  A FD+F ++
Sbjct:  143 WAFPGWIGNGKNWPYDFPDVTAYYVVSWIIGAKQYHNLDIDYIGIWNERAYDIKYIKLLRYTLDKNNLEHVKIIASDNLWQPIAFHMLQDPELLRVVDVIGAHYPGTHAAQDAILTGKTLWASEDYSTYNDEVGGGCWARILNQNYVNGNMTSTISWNMVASYYEQLPFGLEGLMTAKEPWSGNYVVSTPIWITAHTTQFTQPGWYY---LKTVGHLDKGGSYVALTD-RLGNLTIVIETMSHNNSICIRPPLPDYNVSAQYATFYLQGSFKNL-QELQVWYSSLDINN-TKPVTFKKLSPLKVQNGSFTLQLGVNEVYTITTLLTGQKGSFPDPPESKPFPLKYKDDFSVRNPPFSEAPYFADQSGVFEYFTNTSDPGDHVFTFRQVLTQRPITWASDA---NQAISVIGNYQWSNVTVTSDIYIET-PDTGGVFVAARVDQGGSPTDRAKGIFFWV-FADGTYKVTGDLMGQTILCKGLAGVRARSWHTLTLHIDGTNAFGLLNGNPLWK--EVVTGGPLNGWAAIGTSCFEFAQFDNFMIE 673

HSP 2 Score: 100.908 bits (250), Expect = 7.018e-122
Identity = 65/114 (57.02%), Postives = 83/114 (72.81%), Query Frame = 2 
Query:   35 VFLICLKLINANIISVTHSIDNLIYYPK-FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +F++ L L+        +S+D+L    + FDGIGAISGGGATS+LL  Y  P +++ILDYLFKP FGASLH+ KVEIGGD+Q+T+GTE SH HY  + N  RGYEW L+ EAKK
Sbjct:   18 MFILWLVLLLFPGAPAQYSVDDLGGLGRMFDGIGAISGGGATSRLLVNYPEPQRTEILDYLFKPNFGASLHIFKVEIGGDAQTTDGTEPSHMHYPDDQNYFRGYEWWLMKEAKK 131
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. TrEMBL
Match: A0A267ENL7 (Glyco_hydro_59M domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000813g1 PE=4 SV=1)

HSP 1 Score: 473.396 bits (1217), Expect = 1.215e-156
Identity = 251/554 (45.31%), Postives = 346/554 (62.45%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLS--TNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESP---------------TKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            WAFPGWVGN T +PY+ P+  A Y + W++  ++ +N+   Y+G+WNE+ YDV Y++ LR  LD  GF N++++  D  W  A D++ D + +   +A+G HYPGT SSD AK +GKPLW+SEDFST N + G+GCWARILN+N+V+G MTS+IAWNL+AAYYD+LP+ RC LMTA EPWSG+Y+V   +W+T+HT+QF  PGW Y PVG GSG    GGSYV L D   K LTII E MSHD S CIRP+LPP+  K QN+T+K     A +  Q + W++ LS    D S  F  +  +Q   SG++   L VD VVTLST++TA K     PP S PFP  YSD+FQ+Y   + P  LVPQVGSFE+  ++SP                K+ L+QTV+ QP+ WC      ++PIAVIGN+N SD+ +S  ++VPS+     +F+ +RI+  GC  F A G+F WL   S+++ ++ D+   K          Y+ W  +T+ L+       +  Q V     L   +P  GFAGLG++ FGLA F +  +
Sbjct:  141 WAFPGWVGNGTSSPYNYPSLTAKYVLKWVQGAKDYHNLTIDYLGIWNERAYDVEYVRLLRAVLDHAGFKNIRLVVGDGDWGPAHDVLNDPQFAAATFALGAHYPGTLSSDEAKRTGKPLWASEDFSTFNDDIGSGCWARILNRNFVDGGMTSSIAWNLVAAYYDNLPFPRCSLMTANEPWSGHYEVTGPLWMTAHTSQFALPGWYYLPVGGGSGRLPSGGSYVGLTDSNTKQLTIIFETMSHDHSECIRPSLPPYTVKPQNVTIKLAGAFASI-TQLYYWKSVLSYKKGDSSTYFVKQSPLQV-KSGQLTLFLDVDVVVTLSTVATATKGQRSQPPPSKPFPLPYSDNFQNYQPGSNPLNLVPQVGSFEI--VDSPPTVGNGRSIIDNYNVNKQCLMQTVLQQPVDWC----KQSQPIAVIGNYNWSDISVSASVLVPSVNSTTGIFVAARIDQGGCGAFQAKGLFLWLFPASNQYAVTNDLAQTKKIKGGSFVYGYDAWVKLTLTLQSGKYTAKIGNQTVANSETLPGSTPSTGFAGLGSENFGLACFANLSI 686

HSP 2 Score: 111.694 bits (278), Expect = 1.215e-156
Identity = 52/71 (73.24%), Postives = 58/71 (81.69%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            KLLQ Y    + QILDYLF P FGASLH+LKVEIGGDSQSTEGTEASH H+ W++N NRGYEW L+ EAKK
Sbjct:   59 KLLQNYPQNQRDQILDYLFLPNFGASLHMLKVEIGGDSQSTEGTEASHMHHSWDLNYNRGYEWWLMKEAKK 129
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. TrEMBL
Match: A0A267E2J8 (Glyco_hydro_59M domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000813g2 PE=4 SV=1)

HSP 1 Score: 467.618 bits (1202), Expect = 7.173e-155
Identity = 247/553 (44.67%), Postives = 343/553 (62.03%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESP---------------TKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            WAFPGWVGN T +PY+ P+  A Y + W++  ++ +N+   Y+G+WNE+ YDV Y++ LR  LD  GF N++++  D  W  A D++ D + +   +A+G HYPGT SS+ AK +GKPLW+SEDFST N + G+GCWARILN+N+V+G MTS+IAWNL+AAYYD+LP+ RC LMTA EPWSG+Y+V   +W+T+HT+QF  PGW Y P G GSG    GGSYV L D   K LTII E MSHD S CIRP+LPP+  K QN+T+K     A +  Q + W++ LS        +F K       SG++   L VD VVTLST++TA K     PP S PFP  YSD+FQ+Y   + P  LVPQVGSFE+  ++SP                K+ L+QTV+ QP+ WC      ++PIAVIGN+N SD+ +S  ++VPS+     +F+ +RI+  GC  F A G+F WL   S+++ ++ D+   K          Y+ W  +T+ L+       +  Q V  +  L   +P  GFAGLG++ FGLA F +  +
Sbjct:  141 WAFPGWVGNGTSSPYNYPSLTAKYVLKWVQGAKDYHNLTIDYLGIWNERAYDVEYVRLLRAVLDHAGFKNIRLVVGDGDWGPAHDVLNDPQFAAATFALGAHYPGTLSSNEAKRTGKPLWASEDFSTFNDDIGSGCWARILNRNFVDGGMTSSIAWNLVAAYYDNLPFPRCSLMTANEPWSGHYEVTGPLWMTAHTSQFALPGWYYLPAGGGSGRLPSGGSYVGLTDSNTKQLTIIFETMSHDHSECIRPSLPPYTVKPQNVTIKLAGAFASI-TQLYYWKSVLSYKKGVSSSYFVKQSPLQVKSGQLTLFLDVDVVVTLSTVATATKGQRSQPPPSKPFPLPYSDNFQNYQPGSNPLNLVPQVGSFEI--VDSPPTVGNGRSIIDHYNVNKQCLMQTVLQQPVDWC----KQSQPIAVIGNYNWSDISVSASVLVPSVNSTTGIFVAARIDQGGCGAFQAKGLFLWLFPASNQYAVTNDLAQTKKIKGGSFVYGYDAWVKLTLNLQSGKYTAKIGNQTVANLETLPGSTPSTGFAGLGSENFGLACFANLSI 686

HSP 2 Score: 112.079 bits (279), Expect = 7.173e-155
Identity = 52/71 (73.24%), Postives = 58/71 (81.69%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            KLLQ Y    + QILDYLF P FGASLH+LKVEIGGDSQSTEGTEASH H+ W++N NRGYEW L+ EAKK
Sbjct:   59 KLLQNYPQNQRDQILDYLFLPNFGASLHMLKVEIGGDSQSTEGTEASHMHHSWDLNYNRGYEWWLMKEAKK 129
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. TrEMBL
Match: A0A0B7AEQ2 (Glyco_hydro_59M domain-containing protein OS=Arion vulgaris OX=1028688 GN=ORF113654 PE=4 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 4.258e-147
Identity = 243/540 (45.00%), Postives = 349/540 (64.63%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTND-PSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKILV-QTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQ-WYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            W FPGW+G  T +P+  P  LA+Y + W+   +  YN+   YIG+WNEK YD+ YIKTLR  LD+ GF NV II AD KWEIA+DI KD EL+  IYAIGCHYPGT SS  A  +GK LW+SED+ST N E GAGCWARILNQNYVNG MTSTI+WNLI +YY  LPY R GLMTA+EPWSGNY V + +W+++H TQF+  GWKY   G G G    GGSYV L  P+  NLTI++E M+HD SVCIRP LP +   +QNIT+    + A++  Q  +W + LS N  PS++F  ++  +   +G++   L +D +VTL+++   +K  +P PP S PFP  Y+D+F+ Y ++ EP LL PQ GS +V   ++ +  ++  QTV+  PI WC +    + P AVIG+ N +D+ + +   +P +   + VF+ +R+   GC  F+A G++F+  F++S+++++ D+   K+ +Q   NL   Q W+ ++++++ +      DE+ +  +       P  GFAG+GTD FG+A FD+  +
Sbjct:  136 WGFPGWIGQGTRSPFANPMILADYIVRWISGAKIHYNLTIDYIGIWNEKSYDITYIKTLRSVLDQRGFQNVMIIAADKKWEIAADIKKDPELADIIYAIGCHYPGTLSSSDAVSTGKKLWASEDYSTFNDEIGAGCWARILNQNYVNGLMTSTISWNLIGSYYKGLPYYRDGLMTAVEPWSGNYVVQSPIWVSAHITQFSSIGWKYLSHGSGVGKLPNGGSYVGLFSPDGVNLTIVIETMTHDHSVCIRPRLPKYTVTEQNITITLKGHFAQI-TQMNVWYSKLSFNGTPSEMF-VKQSSKTIINGQVNLSLGLDEIVTLTSLPGGQKGSYPEPPASKPFPLPYNDNFEDYTLSQEPFLLTPQTGSLDVVKSQNISHGLVSRQTVLHPPIKWCPNTL--SFPYAVIGDQNWTDLFIEIDFEIPGVNATDGVFVAARVTEGGCQAFIAHGIYFFA-FSNSKYIVANDLARTKVLNQG--NLPPGQGWHKLSLLVQGQKAIGAYDEKGIFNID--IPSYPSSGFAGIGTDNFGIADFDNLHI 666

HSP 2 Score: 108.997 bits (271), Expect = 4.258e-147
Identity = 65/93 (69.89%), Postives = 72/93 (77.42%), Query Frame = 2 
Query:   95 DNLIYYPKFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            D+L Y   FDGIG +SGGGATSKLL  Y    + QILDYLFKP FGASL +LKVEIGGD+QST+GTEASH H  WE N  RGYEW L+VEAKK
Sbjct:   32 DSLGYGRTFDGIGGLSGGGATSKLLVNYPQEQRDQILDYLFKPNFGASLQILKVEIGGDAQSTDGTEASHMHNSWEENYTRGYEWWLMVEAKK 124
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. TrEMBL
Match: A0A210Q2D6 (Galactocerebrosidase OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT17644 PE=4 SV=1)

HSP 1 Score: 437.187 bits (1123), Expect = 1.576e-145
Identity = 233/542 (42.99%), Postives = 335/542 (61.81%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLS--TNDPSKIFHFEKVIQP--DSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+G+   NPY      A Y   WL   +  YN+   YIG+WNE+ YD+ YIKTLR++LD    ++VKI+GAD  WEIAS I KDSEL+  +  IG HYPGT +S  A  +GK LWSSED+ST N   GAGCWARILNQN+VNG MTSTI+WNLIA+YY  LPY  CGLMTA  PW+G+YDV   +WIT+HTTQFT PGW Y   G G G    GGSYV+L+ P+ K+LT+I+E MSHD S+CIRPALP +    QN+T++     AK F+Q  +W + L+   N+ S +F   K + P     G +   + VD + TL+++    K  +P PP   PFP+ YS+DF+ YP+++EP     Q GS+E+ + + P   +L Q V+  P  WC    +N + + ++GN   SD+ +S  I +  +     VF+ +R++  GC ++ A G+FF+L  +  +F+++ D+       +     +   W ++ ++L+  N    +++Q +  +T     +P HGF  +GTD +G+A FD+ Q+K
Sbjct:  131 WAFPGWLGDGGDNPYTNVTRTAEYVTRWLLGAKKFYNLSIDYIGIWNERPYDISYIKTLRKSLDTHQLSHVKIVGADGGWEIASAIEKDSELAAAVDYIGAHYPGTTTSSEALTTGKQLWSSEDYSTYNDNTGAGCWARILNQNFVNGFMTSTISWNLIASYYSALPYPGCGLMTADNPWTGHYDVSAPIWITAHTTQFTAPGWSYLQHGSGVGKLTHGGSYVSLVSPDHKDLTVIIETMSHDHSLCIRPALPAYNVIAQNVTLQLRGVLAK-FQQLNVWYSKLAFGKNETSVLF---KKLSPVKVQDGRVTLSVGVDELYTLTSLPGGTKGTYPAPPARKPFPNTYSEDFEGYPLHSEPVDFAQQTGSYEIVSGDHPHGNVLRQAVLQTPTFWCKADAVN-KSLNLVGNSKWSDVYMSADIAIGPVNGTTGVFVAARVDQGGCQSWKAKGIFFFLFPSEKKFILTNDLARTMPLKEGEATGAVQGWNSLALLLEGSNAVGRLNKQTLFNIT--VPSTPAHGFVAIGTDSYGIADFDNVQIK 665

HSP 2 Score: 111.309 bits (277), Expect = 1.576e-145
Identity = 67/115 (58.26%), Postives = 79/115 (68.70%), Query Frame = 2 
Query:   29 CLVFLICLKLINANIISVTHSIDNLIYYPKFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            CL  L+ +   +   +  +H +       +FDGIG +SGGGATSKLL  Y   Y+ QILDYLFKP F ASL +LKVEIGGDSQSTEGTEASH H EW+ N  RGYEW L+ EAKK
Sbjct:   10 CLSALLSVTFCDDYRVDYSHGLGR-----RFDGIGGLSGGGATSKLLVNYPKDYRDQILDYLFKPNFAASLQILKVEIGGDSQSTEGTEASHMHNEWDENYQRGYEWWLMTEAKK 119
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. TrEMBL
Match: A0A1I8GXE8 (Glyco_hydro_59M domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 432.95 bits (1112), Expect = 1.760e-144
Identity = 234/529 (44.23%), Postives = 322/529 (60.87%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLS--TNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKI------LVQTVIDQPISWCFDMPIN-TRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFA 1935
            WAFPGWVGN T +PY+ P+  A Y + W++  ++ +N+   Y+G+WNE+ YDV Y++ LR  LD  GF N++++  D  W  A D++ D + +   +A+G HYPGT SSD AK +GKPLW+SEDFST N + G+GCWARILN+N+V+G MTS+IAWNL+AAYYD+LP+ RC LMTA EPWSG+Y+V   +W+T+HT+QF  PGW Y PVG GSG    GGSYV L D   K LTII E MSHD S CIRP+LPP+  K QN+T+K     A +  Q + W++ LS    D S  F  +  +Q   SG++   L VD VVTLST++TA K     PP S PFP  YSD+FQ+Y   + P  LVPQ G FE   L     ++      L Q V    ++WC   P+  T P  V+GN+  SD+ +S  ++VPS+     +F+ +RI+  GC  F A G+F WL   S+++ ++ D+   K          Y+ W  +T+ L+       +  Q V     L   +P  GFA
Sbjct:  141 WAFPGWVGNGTSSPYNYPSLTAKYVLKWVQGAKDYHNLTIDYLGIWNERAYDVEYVRLLRAVLDHAGFKNIRLVVGDGDWGPAHDVLNDPQFAAATFALGAHYPGTLSSDEAKRTGKPLWASEDFSTFNDDIGSGCWARILNRNFVDGGMTSSIAWNLVAAYYDNLPFPRCSLMTANEPWSGHYEVTGPLWMTAHTSQFALPGWYYLPVGGGSGRLPSGGSYVGLTDSNTKQLTIIFETMSHDHSECIRPSLPPYTVKPQNVTIKLAGAFASI-TQLYYWKSVLSYKKGDSSTYFVKQSPLQVK-SGQLTLFLDVDVVVTLSTVATATKGQRSQPPPSKPFPLPYSDNFQNYQPGSNPLNLVPQAGFFEAVKLSGGASEVSAQPQALCQVVTLATLNWC---PLRLTSPYTVMGNW--SDISVSASVLVPSVNSTTGIFVAARIDQGGCGAFQAKGLFLWLFPASNQYAVTNDLAQTKKIKGGSFVYGYDAWVKLTLTLQSGKYTAKIGNQTVANSETLPGSTPSTGFA 662

HSP 2 Score: 111.694 bits (278), Expect = 1.760e-144
Identity = 52/71 (73.24%), Postives = 58/71 (81.69%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            KLLQ Y    + QILDYLF P FGASLH+LKVEIGGDSQSTEGTEASH H+ W++N NRGYEW L+ EAKK
Sbjct:   59 KLLQNYPQNQRDQILDYLFLPNFGASLHMLKVEIGGDSQSTEGTEASHMHHSWDLNYNRGYEWWLMKEAKK 129
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Cavefish
Match: galca (galactocerebrosidase [Source:NCBI gene;Acc:103028593])

HSP 1 Score: 360.918 bits (925), Expect = 6.411e-125
Identity = 212/539 (39.33%), Postives = 312/539 (57.88%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVG---TLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDF 1980
            WAFPGW+GN T  PY  P+  ANY ++W+   ++ ++++ +YIG+WNE+ ++  YI  LR  LD+ G  +V II AD  W+I++ ++ D  L+  +  IG HYPGT +   A L+GK LWSSED+ST N + GAGCWARILNQNY+NG MTSTI+WNLIA+YY+DL +   GLMTA EPWSGNY V + +WIT+HTTQFTQPGW Y       GH   GGSYV L D +  NLT+++E MSH+ S CIRPALPP+    Q  T +   + A L  +   W T         +   +       +G     L VD V T++++S  +K ++P PP+S PFP +Y DDF    P  +E      Q G FE     T   P    L Q V  +P++W +D     + I+V+G+ N  D+ +S  I + ++     VF+ +R++ +G +   A GVFFW+ F +  + ++ DI  +K+  +         W+ +T+ +K    +  ++   + +  N     P +G+A +GT  +  A FD+F
Sbjct:  130 WAFPGWIGNGTNWPYTFPDITANYVVSWVIGAKHYHDLDIEYIGIWNERDFNAKYITVLRDALDRAGLKDVSIIAADGNWDISNALLTDPYLNEAVGVIGVHYPGTTTVKEALLTGKRLWSSEDYSTFNNDIGAGCWARILNQNYINGRMTSTISWNLIASYYEDLSFGGDGLMTAEEPWSGNYVVESPIWITAHTTQFTQPGWTY---LDTVGHLAQGGSYVALTDGK-GNLTVVIETMSHNHSQCIRPALPPYSVSPQTATFRLKGSFAAL-TELQQWYTKFDFETKKDVLFKKTNSIKIQNGYFNLTLDVDEVYTITSVSGGQKGLYPDPPISAPFPRKYFDDFNVRNPPFSEAPYFADQTGVFEYFNNLTDPGPHAFTLRQVVTQRPVTWAYDA---DQTISVVGDHNWQDLTVSCDIYIENMN--GGVFIAARVDQAGESVKNAMGVFFWV-FANGTYKVTNDIGGKKVLAEGLSGTQPGIWHTLTLTVKGYFAFGMMNGYPLWK--NAVVLQPKNGWAAIGTHTYEFAQFDNF 655

HSP 2 Score: 108.227 bits (269), Expect = 6.411e-125
Identity = 62/86 (72.09%), Postives = 72/86 (83.72%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIG +SGGGATS+LL  YK PY+SQILDYLFKP FGASLH+LKVEIGGD+Q+T+GTE SH H E + N  RGYEW L+ EAKK
Sbjct:   33 RFDGIGGLSGGGATSRLLINYKEPYRSQILDYLFKPNFGASLHILKVEIGGDAQTTDGTEPSHMHSEKDENYFRGYEWWLMKEAKK 118
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Cavefish
Match: galcb (galactocerebrosidase-like [Source:NCBI gene;Acc:103041717])

HSP 1 Score: 355.525 bits (911), Expect = 9.320e-125
Identity = 214/545 (39.27%), Postives = 305/545 (55.96%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVG---TLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEV--TNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFPGW+G     PY  P+   +Y ++W+   +  ++++  Y+G+WNE+ +D+ YIK LR TLDK G   V+II +D  WE I   ++ D EL   +  +G HYPGT S   A   GK LW+SED+ST N E G GCWARILNQNYVNG M++TI+WNL+A+YY +LP+ R GLMTA EPWSGNY V + +W T+HTTQF+QPGWKY    +  GH   GGSYV L D +  NLTI++E M+HD SVCIRP LPP+    Q  T     + A L  +  LW +         +F  +      S+G     L VD V T++TI T  K  +P PP S+PFP  Y DDF    P  +E      Q G FE     T   P    L Q V  +PI+W  D     + ++VIG+++  +M +S  + +        VF+ +R++  G +   A G+FFW+      + +++D++   +  +       N W  +T+ +K      +V  +L   +   N  D  P +G+A +GT  F LA FD+F ++
Sbjct:  141 WAFPGWIGRGVNWPYDFPDVTVSYIVSWIIGAKQYHDLDIDYVGIWNERNFDIKYIKLLRYTLDKAGLERVRIIASDNLWEPITLSVLGDEELREAVDVLGAHYPGTTSVPAAVKMGKQLWASEDYSTFNDEVGGGCWARILNQNYVNGLMSATISWNLVASYYQNLPFGRDGLMTAQEPWSGNYVVESPIWTTAHTTQFSQPGWKY---LQTVGHLTHGGSYVALTD-QKGNLTIVIETMTHDHSVCIRPPLPPYNVTAQTATFSLKGSFASL-SELQLWNSKFDFKTKKPVFFQKLAPIKVSNGSFTLDLDVDEVYTVTTIPTGLKGSYPEPPPSSPFPKTYKDDFNVKNPSFSEAPDFADQTGVFEYFMNLTDPGPHVYTLRQVVTQRPITWVADA---DQTVSVIGDYSWQNMTVSCDVFMEK-ENSGGVFVAARLDKGGGSVRNARGIFFWV-LADGTYRVTSDLSGLSVLAEGLSGTRANTWNTLTLTVKGN----FVSGELNGYLLWKNTVDLVPKNGWAAIGTSSFELAQFDNFAVQ 671

HSP 2 Score: 112.849 bits (281), Expect = 9.320e-125
Identity = 72/122 (59.02%), Postives = 87/122 (71.31%), Query Frame = 2 
Query:   17 ISNYCLVFLI--CLKLINANIISVTHSIDNLIYYPK-FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +S  C + LI  C  L +A      +SID+     + FDGIG +SGGGATS+LL  Y  PY++QILDYLFKP FGASLH+LKVEIGGD+QST+GTE SH HYE + N  RGYEW L+ EAKK
Sbjct:   12 VSRLCTLLLISACFTLTSAE----HYSIDDQAGLGRVFDGIGGLSGGGATSRLLINYAEPYRTQILDYLFKPNFGASLHILKVEIGGDAQSTDGTEPSHMHYEDDENYFRGYEWWLMTEAKK 129
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Cavefish
Match: galca (galactocerebrosidase [Source:NCBI gene;Acc:103028593])

HSP 1 Score: 339.347 bits (869), Expect = 1.980e-118
Identity = 209/540 (38.70%), Postives = 303/540 (56.11%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVG---TLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEV-TNLYDRSPIHGFAGLGTDMFGLAYFDDF 1980
            WAFPGW+GN T  PY  P+  ANY ++W+   ++ ++++ +YIG+             LR  LD+ G  +V II AD  W+I++ ++ D  L+  +  IG HYPGT +   A L+GK LWSSED+ST N + GAGCWARILNQNY+NG MTSTI+WNLIA+YY+DL +   GLMTA EPWSGNY V + +WIT+HTTQFTQPGW Y       GH   GGSYV L D +  NLT+++E MSH+ S CIRPALPP+    Q  T +   + A L  +   W T         +   +       +G     L VD V T++++S  +K ++P PP+S PFP +Y DDF    P  +E      Q G FE     T   P    L Q V  +P++W +D     + I+V+G+ N  D+ +S  I + ++     VF+ +R++ +G +   A GVFFW+ F +  + ++ DI  +K+  +         W+ +T+ +K   G+F         +  N     P +G+A +GT  +  A FD+F
Sbjct:  130 WAFPGWIGNGTNWPYTFPDITANYVVSWVIGAKHYHDLDIEYIGV-------------LRDALDRAGLKDVSIIAADGNWDISNALLTDPYLNEAVGVIGVHYPGTTTVKEALLTGKRLWSSEDYSTFNNDIGAGCWARILNQNYINGRMTSTISWNLIASYYEDLSFGGDGLMTAEEPWSGNYVVESPIWITAHTTQFTQPGWTY---LDTVGHLAQGGSYVALTDGK-GNLTVVIETMSHNHSQCIRPALPPYSVSPQTATFRLKGSFAAL-TELQQWYTKFDFETKKDVLFKKTNSIKIQNGYFNLTLDVDEVYTITSVSGGQKGLYPDPPISAPFPRKYFDDFNVRNPPFSEAPYFADQTGVFEYFNNLTDPGPHAFTLRQVVTQRPVTWAYDA---DQTISVVGDHNWQDLTVSCDIYIENMN--GGVFIAARVDQAGESVKNAMGVFFWV-FANGTYKVTNDIGGKKVLAEGLSGTQPGIWHTLTLTVK---GYFAFGMMNGYPLWKNAVVLQPKNGWAAIGTHTYEFAQFDNF 642

HSP 2 Score: 108.227 bits (269), Expect = 1.980e-118
Identity = 62/86 (72.09%), Postives = 72/86 (83.72%), Query Frame = 2 
Query:  116 KFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +FDGIG +SGGGATS+LL  YK PY+SQILDYLFKP FGASLH+LKVEIGGD+Q+T+GTE SH H E + N  RGYEW L+ EAKK
Sbjct:   33 RFDGIGGLSGGGATSRLLINYKEPYRSQILDYLFKPNFGASLHILKVEIGGDAQTTDGTEPSHMHSEKDENYFRGYEWWLMKEAKK 118
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Cavefish
Match: galcb (galactocerebrosidase-like [Source:NCBI gene;Acc:103041717])

HSP 1 Score: 256.529 bits (654), Expect = 5.040e-95
Identity = 126/249 (50.60%), Postives = 167/249 (67.07%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCI 1119
            WAFPGW+G     PY  P+   +Y ++W+   +  ++++  Y+G+WNE+ +D+ YIK LR TLDK G   V+II +D  WE I   ++ D EL   +  +G HYPGT S   A   GK LW+SED+ST N E G GCWARILNQNYVNG M++TI+WNL+A+YY +LP+ R GLMTA EPWSGNY V + +W T+HTTQF+QPGWKY    +  GH   GGSYV L D +  NLTI++E M  + S  +
Sbjct:  141 WAFPGWIGRGVNWPYDFPDVTVSYIVSWIIGAKQYHDLDIDYVGIWNERNFDIKYIKLLRYTLDKAGLERVRIIASDNLWEPITLSVLGDEELREAVDVLGAHYPGTTSVPAAVKMGKQLWASEDYSTFNDEVGGGCWARILNQNYVNGLMSATISWNLVASYYQNLPFGRDGLMTAQEPWSGNYVVESPIWTTAHTTQFSQPGWKY---LQTVGHLTHGGSYVALTD-QKGNLTIVIETMVSNGSFTL 385

HSP 2 Score: 112.849 bits (281), Expect = 5.040e-95
Identity = 72/122 (59.02%), Postives = 87/122 (71.31%), Query Frame = 2 
Query:   17 ISNYCLVFLI--CLKLINANIISVTHSIDNLIYYPK-FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +S  C + LI  C  L +A      +SID+     + FDGIG +SGGGATS+LL  Y  PY++QILDYLFKP FGASLH+LKVEIGGD+QST+GTE SH HYE + N  RGYEW L+ EAKK
Sbjct:   12 VSRLCTLLLISACFTLTSAE----HYSIDDQAGLGRVFDGIGGLSGGGATSRLLINYAEPYRTQILDYLFKPNFGASLHILKVEIGGDAQSTDGTEPSHMHYEDDENYFRGYEWWLMTEAKK 129

HSP 3 Score: 78.5666 bits (192), Expect = 1.219e-14
Identity = 71/242 (29.34%), Postives = 116/242 (47.93%), Query Frame = 1 
Query: 1282 SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEVG---TLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEV--TNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            S+G     L VD V T++TI T  K  +P PP S+PFP  Y DDF    P  +E      Q G FE     T   P    L Q V  +PI+W  D     + ++VIG+++  +M +S  + +        VF+ +R++  G +   A G+FFW+      + +++D++   +  +       N W  +T+ +K      +V  +L   +   N  D  P +G+A +GT  F LA FD+F ++
Sbjct:  379 SNGSFTLDLDVDEVYTVTTIPTGLKGSYPEPPPSSPFPKTYKDDFNVKNPSFSEAPDFADQTGVFEYFMNLTDPGPHVYTLRQVVTQRPITWVADAD---QTVSVIGDYSWQNMTVSCDVFMEK-ENSGGVFVAARLDKGGGSVRNARGIFFWV-LADGTYRVTSDLSGLSVLAEGLSGTRANTWNTLTLTVKGN----FVSGELNGYLLWKNTVDLVPKNGWAAIGTSSFELAQFDNFAVQ 611
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Sea Lamprey
Match: galca (galactosylceramidase a [Source:NCBI gene;Acc:449649])

HSP 1 Score: 397.127 bits (1019), Expect = 1.445e-132
Identity = 238/543 (43.83%), Postives = 314/543 (57.83%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLS-TNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQ-SYPINAEPDLLVPQVGSFEV-GTLESPTKKI--LVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
            WAFP WVG     PY  P   A Y ++W+   +  + ++  Y+G+WNE++YD+ YIK LRQTLDK G   VKII AD KWEIASDI+ DSEL+  I  IG HYPGT S+  A  +GK LWSSEDFST N E GAGCWARILNQNYVNGNMTSTI+WNL+A+YY  LP+ R GLMTA EPWSG Y V   +WI++HTTQFT+PGW Y    +  GH   GGSYV L D    NLTI++E M+H+ SVCIRP LPPF    QN+T +   + A +     +W + L  T  PS +F     I+    G     L VD V T +T+ T  +  +P PP S PFPS Y+D F  + P  +E      Q G FE+   L  P   +  + Q +  +PI+W  D     +PI VIGN N SD+++S  + +        VF+  R+N +G     A GVFFW+ F +  F ++ D+    ++           WY MT+ L+ ++    V+ QLV     +    P  G+   GT  F  A FD+F ++
Sbjct:  156 WAFPAWVGGEFKFPYKYPEVTAGYVVSWVLGAKQYHGLDIDYVGIWNERKYDINYIKVLRQTLDKVGLGWVKIIAADNKWEIASDILHDSELAEAISVIGAHYPGTKSTVDAVRTGKVLWSSEDFSTFNDETGAGCWARILNQNYVNGNMTSTISWNLVASYYGSLPFGRDGLMTAEEPWSGFYSVDGPIWISAHTTQFTRPGWHY---LQADGHLKAGGSYVALTDGS-GNLTIVIETMTHNHSVCIRPPLPPFTVSPQNVTFRLTGSFAHV-ASLQVWHSRLYFTGKPSVLFEPLSPIKKVDKGSFTLSLDVDEVYTFTTLLTGNRGNYPRPPASQPFPSLYNDSFDVATPPFSEAPNFADQTGVFELFRNLSDPGPHVFTMRQVITTRPITWAEDA---KQPITVIGNHNWSDVLVSCDVFMEDAEQ-GGVFIAGRVNKAGNDIPFARGVFFWI-FANGTFALTNDLAGRFVFMSGTAPTKPWTWYTMTLSLQGDSVVAVVENQLVMTQEKII--MPTRGWVAFGTHTFQNAQFDNFYVQ 686

HSP 2 Score: 94.7449 bits (234), Expect = 1.445e-132
Identity = 45/71 (63.38%), Postives = 54/71 (76.06%), Query Frame = 2 
Query:  161 KLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            +LL  Y  P++S ILDYLFKP FGASLH+LKVEIGGD+Q+T+GTE SH     + N  RGYEW L+ EAKK
Sbjct:   74 RLLVNYPEPHRSAILDYLFKPNFGASLHILKVEIGGDAQTTDGTEPSHMRDLGDENYFRGYEWWLMKEAKK 144
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Medaka
Match: galcb (galactosylceramidase [Source:NCBI gene;Acc:101163287])

HSP 1 Score: 366.696 bits (940), Expect = 5.818e-126
Identity = 217/546 (39.74%), Postives = 318/546 (58.24%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWE-IASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLW--QTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDF--QSYPINAEPDLLVPQVGSFE--VGTLE-SPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            W+FPGW+G+    PY  P+  ANY + W+   +  +++  QY+G+WNE+ +DV YIK LR TLDK G   V+II +D  WE IA  ++ D ELS  +  IG HYPGT +   A  + K LW+SED+ST N   GAGCWARILNQNYVNG MTSTI+WNL+A+YY++LP+ RCGLMTA EPWSGNY V   +WIT+HTTQFTQPGW Y    +  GH   GGSYV L D +  NLT+++E M+HD S CIRP LP F    Q  T     + A + K+  +W  Q +  T  PS    FEK+      +G     L  D V TL+T++T +K  +  PP S  FP  Y D+F  Q  P +  P+    Q G FE  V T +  P    + Q V  +P++W  D     + I+VIG++   ++ ++  + + +++    +F+ +R++  G     A GVFFW+ F +  + +++DI  + +  + +       WY +++ ++ +     ++ +++   TN     P +G+A +GT  F LA FD+F +
Sbjct:  133 WSFPGWLGHGKDWPYDFPDVTANYVVKWILGAKKYHDLHIQYVGIWNERNFDVKYIKLLRYTLDKSGLEEVRIIASDNLWEPIAQSLLLDPELSGAVDVIGAHYPGTVTVPQALKTLKKLWASEDYSTYNDAMGAGCWARILNQNYVNGQMTSTISWNLVASYYEELPFGRCGLMTAQEPWSGNYVVSPPIWITAHTTQFTQPGWTY---LRTVGHLAQGGSYVALTDGK-GNLTVVIETMTHDHSACIRPPLPAFNLTSQTATFNLRGSFASI-KELQVWRSQFNFKTQKPS---FFEKLTAVKLVNGSFTLDLAEDEVYTLTTVTTGRKGSYADPPPSARFPKAYKDNFDVQDPPFSEAPN-FADQTGVFEYFVNTTDPGPHVFTMRQVVTQRPVTWTADA---DQTISVIGDYKWQNLSVTCDVFMENVKN-GGIFVAARVSKGGQDVRRAKGVFFWV-FVNGTYKVTSDIGGQAVLAEGKSGTEAFTWYTLSLTVEGQKATGMLNGKVLW--TNSVQLQPPNGWAAIGTASFELAQFDNFSV 662

HSP 2 Score: 105.916 bits (263), Expect = 5.818e-126
Identity = 61/85 (71.76%), Postives = 70/85 (82.35%), Query Frame = 2 
Query:  119 FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            FDGIG +SGGGATS+LL  Y  P++SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HYE + N  RGYEW L+ EAKK
Sbjct:   37 FDGIGGLSGGGATSRLLVNYPEPHRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYEDDENYFRGYEWWLMKEAKK 121
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Medaka
Match: galcb (galactosylceramidase [Source:NCBI gene;Acc:101163287])

HSP 1 Score: 363.229 bits (931), Expect = 5.315e-125
Identity = 214/545 (39.27%), Postives = 313/545 (57.43%), Query Frame = 1 
Query:  376 WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLW--QTDLSTNDPSKIFHFEKVIQPD-SSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDF--QSYPINAEPDLLVPQVGSFE--VGTLE-SPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQM 1986
            W+FPGW+G+    PY  P+  ANY + W+   +  +++  QY+G+WNE+ +DV YIK LR  LD+ G   V II AD  W IA  +I D  L   +  IG HYPGT +   A  + K LW+SED+ST N   GAGCWARILNQNYVNG MTSTI+WNL+A+YY++LP+ RCGLMTA EPWSGNY V   +WIT+HTTQFTQPGW Y    +  GH   GGSYV L D +  NLT+++E M+HD S CIRP LP F    Q  T     + A + K+  +W  Q +  T  PS    FEK+      +G     L  D V TL+T++T +K  +  PP S  FP  Y D+F  Q  P +  P+    Q G FE  V T +  P    + Q V  +P++W  D     + I+VIG++   ++ ++  + + +++    +F+ +R++  G     A GVFFW+ F +  + +++DI  + +  + +       WY +++ ++ +     ++ +++   TN     P +G+A +GT  F LA FD+F +
Sbjct:  133 WSFPGWLGHGKDWPYDFPDVTANYVVKWILGAKKYHDLHIQYVGIWNERNFDVKYIKVLRNMLDRVGLKGVGIIAADGDWSIAKSMISDPYLYNSVDVIGAHYPGTVTVPQALKTLKKLWASEDYSTYNDAMGAGCWARILNQNYVNGQMTSTISWNLVASYYEELPFGRCGLMTAQEPWSGNYVVSPPIWITAHTTQFTQPGWTY---LRTVGHLAQGGSYVALTDGK-GNLTVVIETMTHDHSACIRPPLPAFNLTSQTATFNLRGSFASI-KELQVWRSQFNFKTQKPS---FFEKLTAVKLVNGSFTLDLAEDEVYTLTTVTTGRKGSYADPPPSARFPKAYKDNFDVQDPPFSEAPN-FADQTGVFEYFVNTTDPGPHVFTMRQVVTQRPVTWTADA---DQTISVIGDYKWQNLSVTCDVFMENVKN-GGIFVAARVSKGGQDVRRAKGVFFWV-FVNGTYKVTSDIGGQAVLAEGKSGTEAFTWYTLSLTVEGQKATGMLNGKVLW--TNSVQLQPPNGWAAIGTASFELAQFDNFSV 661

HSP 2 Score: 105.916 bits (263), Expect = 5.315e-125
Identity = 61/85 (71.76%), Postives = 70/85 (82.35%), Query Frame = 2 
Query:  119 FDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            FDGIG +SGGGATS+LL  Y  P++SQILDYLFKP FGASLH+LKVEIGGD Q+T+GTE SH HYE + N  RGYEW L+ EAKK
Sbjct:   37 FDGIGGLSGGGATSRLLVNYPEPHRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYEDDENYFRGYEWWLMKEAKK 121
[Close]
BLAST of Glyco_hydro_59M domain-containing protein vs. Planmine SMEST
Match: SMESG000068390.1 (SMESG000068390.1)

HSP 1 Score: 1061.21 bits (2743), Expect = 0.000e+0
Identity = 538/539 (99.81%), Postives = 538/539 (99.81%), Query Frame = 1 
Query:  373 TWAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVXXXXXXXXXXXSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHXXXXXXXXXXSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 1989
             WAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVGKGSGHFDGGGSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHPPPPVSTPFPSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK
Sbjct:  131 AWAFPGWVGNFTGNPYHLPNSLANYTINWLKCGRNIYNVEFQYIGLWNEKRYDVVYIKTLRQTLDKEGFANVKIIGADIKWEIASDIIKDSELSRCIYAIGCHYPGTNSSDTAKLSGKPLWSSEDFSTSNIEKGAGCWARILNQNYVNGNMTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQFTQPGWKYAPVGKGSGHFDGGGSYVTLIDPEMKNLTIILENMSHDSSVCIRPALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVIQPDSSGEIKFVLPVDHVVTLSTISTAKKSIHPPPPVSTPFPSEYSDDFQSYPINAEPDLLVPQVGSFEVGTLESPTKKILVQTVIDQPISWCFDMPINTRPIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVFFWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYVDEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMK 669

HSP 2 Score: 220.32 bits (560), Expect = 1.189e-61
Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 2 
Query:   14 MISNYCLVFLICLKLINANIISVTHSIDNLIYYPKFDXXXXXXXXXXXXKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 373
            MISNYCLVFLICLKLINANIISVTHSI+NLIYYPKFDGIGAISGGGATSKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK
Sbjct:    1 MISNYCLVFLICLKLINANIISVTHSINNLIYYPKFDGIGAISGGGATSKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEASHSHYEWEVNCNRGYEWQLIVEAKK 120
The following BLAST results are available for this feature:
BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GALC1.466e-12240.66galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:... [more]
GALC3.511e-12240.66galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:... [more]
GALC1.483e-11942.28galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:... [more]
GALC1.616e-11340.26galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:... [more]
GALC2.353e-9354.36galactosylceramidase [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C29E4.108.862e-6532.13Putative galactocerebrosidase [Source:UniProtKB/S... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
galcb2.860e-12440.48galactosylceramidase b [Source:ZFIN;Acc:ZDB-GENE-0... [more]
galca6.251e-12440.81galactosylceramidase a [Source:NCBI gene;Acc:44964... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vdac24.665e-12540.88voltage-dependent anion channel 2 [Source:Xenbase;... [more]
vdac21.987e-12240.63voltage-dependent anion channel 2 [Source:Xenbase;... [more]
vdac23.930e-12240.63voltage-dependent anion channel 2 [Source:Xenbase;... [more]
vdac24.798e-12240.63voltage-dependent anion channel 2 [Source:Xenbase;... [more]
vdac25.390e-12240.63voltage-dependent anion channel 2 [Source:Xenbase;... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Galc2.634e-12840.18galactosylceramidase [Source:MGI Symbol;Acc:MGI:95... [more]
Galc2.676e-10440.41galactosylceramidase [Source:MGI Symbol;Acc:MGI:95... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P54818|GALC_MOUSE1.602e-12740.18Galactocerebrosidase OS=Mus musculus OX=10090 GN=G... [more]
sp|Q498K0|GALC_XENLA4.650e-12640.33Galactocerebrosidase OS=Xenopus laevis OX=8355 GN=... [more]
sp|Q5SNX7|GALC_DANRE3.853e-12340.81Galactocerebrosidase OS=Danio rerio OX=7955 GN=gal... [more]
sp|P54803|GALC_HUMAN6.457e-12240.66Galactocerebrosidase OS=Homo sapiens OX=9606 GN=GA... [more]
sp|Q0VA39|GALC_XENTR7.018e-12240.63Galactocerebrosidase OS=Xenopus tropicalis OX=8364... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267ENL71.215e-15645.31Glyco_hydro_59M domain-containing protein OS=Macro... [more]
A0A267E2J87.173e-15544.67Glyco_hydro_59M domain-containing protein OS=Macro... [more]
A0A0B7AEQ24.258e-14745.00Glyco_hydro_59M domain-containing protein OS=Arion... [more]
A0A210Q2D61.576e-14542.99Galactocerebrosidase OS=Mizuhopecten yessoensis OX... [more]
A0A1I8GXE81.760e-14444.23Glyco_hydro_59M domain-containing protein OS=Macro... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
galca6.411e-12539.33galactocerebrosidase [Source:NCBI gene;Acc:1030285... [more]
galcb9.320e-12539.27galactocerebrosidase-like [Source:NCBI gene;Acc:10... [more]
galca1.980e-11838.70galactocerebrosidase [Source:NCBI gene;Acc:1030285... [more]
galcb5.040e-9550.60galactocerebrosidase-like [Source:NCBI gene;Acc:10... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
galca1.445e-13243.83galactosylceramidase a [Source:NCBI gene;Acc:44964... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glyco_hydro_59M domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
galcb5.818e-12639.74galactosylceramidase [Source:NCBI gene;Acc:1011632... [more]
galcb5.315e-12539.27galactosylceramidase [Source:NCBI gene;Acc:1011632... [more]
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BLAST of Glyco_hydro_59M domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000068390.11.189e-6199.81SMESG000068390.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019176 ID=SMED30019176|Name=Glyco_hydro_59M domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2333bp
TAAATCTTATATGATGATTTCCAATTATTGTCTTGTTTTTCTTATATGTT
TAAAATTAATTAACGCAAATATTATATCTGTGACTCACAGCATTGACAAT
TTGATATATTATCCAAAATTTGACGGGATCGGAGCAATTAGTGGAGGAGG
AGCTACGAGCAAATTATTGCAAACATACAAAAACCCATACAAGAGTCAAA
TTTTAGATTATTTATTCAAACCACAGTTTGGTGCTTCTTTGCACCTGTTG
AAAGTGGAAATCGGAGGTGATTCCCAAAGTACCGAAGGAACTGAAGCATC
ACATAGCCATTATGAGTGGGAGGTTAATTGCAACAGGGGATATGAATGGC
AGTTGATTGTTGAAGCTAAAAAACTTGGGCTTTTCCTGGATGGGTTGGTA
ATTTTACGGGAAATCCTTATCACTTACCAAATTCACTAGCTAATTATACT
ATTAATTGGTTAAAATGTGGCCGTAACATTTACAATGTTGAATTTCAATA
TATTGGTCTATGGAATGAAAAGCGATATGATGTCGTTTATATAAAGACTC
TTAGACAAACTCTTGACAAAGAAGGCTTTGCCAATGTTAAGATAATTGGA
GCAGATATAAAATGGGAAATCGCATCGGATATTATTAAGGATTCCGAATT
ATCCAGATGTATTTATGCTATTGGTTGTCATTATCCTGGCACAAATTCAT
CTGATACGGCAAAATTATCAGGAAAACCCCTTTGGTCATCTGAAGATTTC
TCCACCTCGAATATTGAAAAAGGCGCTGGCTGTTGGGCGAGAATACTTAA
TCAAAACTATGTCAATGGAAACATGACTAGCACAATTGCATGGAATTTGA
TTGCGGCTTATTATGATGATTTACCATACTCTCGATGTGGTCTAATGACT
GCAATTGAACCCTGGTCTGGAAATTATGACGTACCAACTACGGTTTGGAT
TACCTCTCACACTACTCAATTTACACAGCCAGGTTGGAAGTATGCACCAG
TTGGAAAGGGATCAGGTCATTTTGACGGTGGCGGATCGTATGTTACTCTT
ATTGACCCAGAAATGAAAAATTTGACAATAATTTTAGAAAATATGAGTCA
TGATAGTTCTGTATGTATTCGTCCAGCGCTGCCGCCATTTATTGCAAAAG
ATCAAAACATTACTGTGAAATTTGATTCAAATACAGCAAAACTTTTTAAA
CAATTTTTCCTTTGGCAAACTGACTTATCTACGAATGATCCTTCTAAAAT
CTTTCATTTTGAGAAAGTTATTCAGCCGGATTCATCTGGAGAAATAAAGT
TTGTCTTACCAGTTGACCATGTCGTAACTTTAAGCACGATTTCCACGGCT
AAGAAATCTATTCACCCCCCTCCCCCAGTTTCAACGCCATTTCCTTCTGA
GTATTCGGATGATTTTCAAAGCTATCCTATCAATGCAGAACCTGATTTAC
TCGTACCACAAGTTGGATCTTTTGAAGTAGGTACTTTGGAATCTCCAACA
AAGAAAATTCTTGTACAAACCGTTATTGATCAACCTATTTCATGGTGCTT
TGATATGCCAATCAACACCAGACCAATTGCAGTTATTGGAAATTTTAACA
AATCAGATATGATCCTTTCTGTTATGATCATGGTACCTTCACTTCGACCA
GTGAATTCCGTGTTTCTTGGATCACGAATCAATAATAGTGGCTGTACAAC
ATTTTTAGCAGGGGGTGTATTTTTTTGGCTGAATTTTACTTCTTCAGAAT
TTCTCATTTCAACTGATATTAATTCTGAGAAAATTTATGATCAGCGAAGG
ATCAATCTCTCGTATAATCAATGGTATAATATGACAATTATCCTAAAAAA
AGAAAATGGTTGGTTCTATGTCGATGAACAATTAGTATGTGAGGTGACCA
ACCTCTATGATCGCAGTCCGATCCACGGATTTGCTGGTCTTGGTACAGAC
ATGTTTGGCCTGGCCTATTTCGACGATTTTCAAATGAAGCTTATCTAAAA
TTACTTATTTATATTAATTTTGATTGTTGTTATTATAAACTGGGTTGTCT
TTTTTATTTTAAAAAGTTTTTATAATTGTCTGACTTTCTTTTAACCTTCT
TTGCATTTTTGATTAAAAAAAATTAAAAAGACAAAAATGTGATTTCGTGG
TCGCCGTTTTTATATTTTAACACTCCTTTTTTATTTGTTATAATTTTTAA
AAACATTTCTATCGCATTAATGAATTATAATAATACTGAGTCTGTTCATG
ATTCATAGCATCAAATTTGTACTATAATTTTTCATGTTTGGATTATTATT
AGAATAAAAAAAATAATCCAAGGAAGAAAACAG
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protein sequence of SMED30019176-orf-1

>SMED30019176-orf-1 ID=SMED30019176-orf-1|Name=SMED30019176-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=146bp
KSYMMISNYCLVFLICLKLINANIISVTHSIDNLIYYPKFDGIGAISGGG
ATSKLLQTYKNPYKSQILDYLFKPQFGASLHLLKVEIGGDSQSTEGTEAS
HSHYEWEVNCNRGYEWQLIVEAKKLGLFLDGLVILREILITYQIH*
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protein sequence of SMED30019176-orf-2

>SMED30019176-orf-2 ID=SMED30019176-orf-2|Name=SMED30019176-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=392bp
MTSTIAWNLIAAYYDDLPYSRCGLMTAIEPWSGNYDVPTTVWITSHTTQF
TQPGWKYAPVGKGSGHFDGGGSYVTLIDPEMKNLTIILENMSHDSSVCIR
PALPPFIAKDQNITVKFDSNTAKLFKQFFLWQTDLSTNDPSKIFHFEKVI
QPDSSGEIKFVLPVDHVVTLSTISTAKKSIHPPPPVSTPFPSEYSDDFQS
YPINAEPDLLVPQVGSFEVGTLESPTKKILVQTVIDQPISWCFDMPINTR
PIAVIGNFNKSDMILSVMIMVPSLRPVNSVFLGSRINNSGCTTFLAGGVF
FWLNFTSSEFLISTDINSEKIYDQRRINLSYNQWYNMTIILKKENGWFYV
DEQLVCEVTNLYDRSPIHGFAGLGTDMFGLAYFDDFQMKLI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003824catalytic activity
GO:0004336galactosylceramidase activity
GO:0016787hydrolase activity
GO:0016798hydrolase activity, acting on glycosyl bonds
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR035394Glyco_hydro_59_dom
IPR001286Glyco_hydro_59
IPR013781Glyco_hydro_catalytic_dom
IPR017853Glycoside_hydrolase_SF
Vocabulary: biological process
TermDefinition
GO:0006683galactosylceramide catabolic process
GO:0005975carbohydrate metabolic process
GO:0006629lipid metabolic process
GO:0006665sphingolipid metabolic process
GO:0008152metabolic process
GO:0016042lipid catabolic process
Vocabulary: cellular component
TermDefinition
GO:0005764lysosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001286Glycoside hydrolase, family 59PFAMPF02057Glyco_hydro_59coord: 1..49
e-value: 2.1E-13
score: 50.2
IPR001286Glycoside hydrolase, family 59PANTHERPTHR15172GALACTOCEREBROSIDASEcoord: 1..389
NoneNo IPR availableGENE3DG3DSA:3.20.20.80coord: 1..188
e-value: 1.3E-60
score: 206.7
NoneNo IPR availableGENE3DG3DSA:2.60.120.560coord: 189..389
e-value: 4.8E-36
score: 126.6
IPR035394Glycosyl hydrolase family 59, central domainPFAMPF17387Glyco_hydro_59Mcoord: 62..176
e-value: 1.8E-27
score: 95.7