ML domain-containing protein

Overview


Name	ML domain-containing protein
Smed ID	SMED30019175
Length (bp)	543

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of ML domain-containing protein (SMED30019175) t-SNE clustered cells

Violin plots show distribution of expression levels for ML domain-containing protein (SMED30019175) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ML domain-containing protein (SMED30019175) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ML domain-containing protein (SMED30019175) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019175

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
prepharyngeal region	SMED30019175	SMESG000031788.1	SmedASXL_004189	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
gut	SMED30019175	SMESG000031788.1	dd_Smed_v4_73_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019175	SMESG000031788.1	Contig47095	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019175	SMESG000031788.1	Contig47095	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30019175	SMESG000031788.1	dd_Smed_v6_73_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30019175	SMESG000031788.1	OX_Smed_1.0.02493	ox_Smed_v2	PMID:24238224 Kao et al., 2013
goblet cell	SMED30019175	SMESG000031788.1	dd_Smed_v6_73_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of ML domain-containing protein vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 105.145 bits (261), Expect = 1.543e-28
Identity = 52/132 (39.39%), Postives = 77/132 (58.33%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  IV+
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVS 149

[Close]

BLAST of ML domain-containing protein vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 105.145 bits (261), Expect = 2.623e-28
Identity = 52/132 (39.39%), Postives = 77/132 (58.33%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  IV+
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVS 149

[Close]

BLAST of ML domain-containing protein vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 104.76 bits (260), Expect = 9.859e-28
Identity = 52/131 (39.69%), Postives = 76/131 (58.02%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  IV
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIV 148

[Close]

BLAST of ML domain-containing protein vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 103.99 bits (258), Expect = 1.352e-27
Identity = 51/131 (38.93%), Postives = 76/131 (58.02%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  I+
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIM 148

[Close]

BLAST of ML domain-containing protein vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 102.064 bits (253), Expect = 1.797e-27
Identity = 50/129 (38.76%), Postives = 75/129 (58.14%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAA 507
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P +
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVS 146

[Close]

BLAST of ML domain-containing protein vs. Ensembl Celegans
Match: heh-1 (pep chromosome:WBcel235:III:3198477:3199516:-1 gene:WBGene00006452.1 transcript:R148.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:heh-1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.262e-12
Identity = 35/104 (33.65%), Postives = 52/104 (50.00%), Query Frame = 1

Query:  205 CQLKKGTTPQLTIKYKALEDTKSGTVSVHGKI-GPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIR-LTVKWELVDDQGNDLICITFPAAI 510
            C  KKG+ P + I +K  +DT     SV  K+ G   V FP  +SD C +    CP++ GE   +  SI++  ++PA   + V W+L        +CI F A I
Sbjct:   50 CLFKKGSRPIIQIAFKPSKDTDKLKTSVRAKVGGSAMVDFPQTNSDACTYGVK-CPVSAGENQIFEQSISITENHPAGEVIQVNWQLTRPDSGKEVCIIFLAEI 152

[Close]

BLAST of ML domain-containing protein vs. Ensembl Fly
Match: Npc2a (gene:FBgn0031381 transcript:FBtr0077816)

HSP 1 Score: 98.9821 bits (245), Expect = 1.821e-26
Identity = 51/127 (40.16%), Postives = 70/127 (55.12%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCN--EQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            + DCGSK GK   V I  C+  +  C LK+ TT   +I +   E+  +    VHGK+  I +PFPL + D C  S   CP+   E   Y+ ++ VL SYP + + VKWEL D  G D+IC+  PA I
Sbjct:   21 FSDCGSKTGKFTRVAIEGCDTTKAECILKRNTTVSFSIDFALAEEATAVKTVVHGKVLGIEMPFPLANPDACVDSGLKCPLEKDESYRYTATLPVLRSYPKVSVLVKWELQDQDGADIICVEIPAKI 147

[Close]

BLAST of ML domain-containing protein vs. Ensembl Fly
Match: Npc2b (gene:FBgn0038198 transcript:FBtr0082888)

HSP 1 Score: 57.7658 bits (138), Expect = 1.353e-10
Identity = 39/130 (30.00%), Postives = 53/130 (40.77%), Query Frame = 1

Query:  151 SKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDS----------DLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            SK      V I+ C +  C LK+ T   + +K +   D +  T  + G I  + VP P P            D        CP+  G+  TY NS  +L  YP + L + W L D  G D  C   PA I
Sbjct:   31 SKALAAGDVSISNCPKSKCILKRNTEASIQMKIRPERDFQELTSDIQGII--LDVPLPFPGYYGTSACPHIYDEAGEKKVGCPLKAGQVYTYKNSFKILPVYPTVSLEIHWGLGDKHG-DAACFQIPAKI 157

[Close]

BLAST of ML domain-containing protein vs. Ensembl Fly
Match: Npc2b (gene:FBgn0038198 transcript:FBtr0082889)

HSP 1 Score: 57.7658 bits (138), Expect = 1.353e-10
Identity = 39/130 (30.00%), Postives = 53/130 (40.77%), Query Frame = 1

Query:  151 SKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDS----------DLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            SK      V I+ C +  C LK+ T   + +K +   D +  T  + G I  + VP P P            D        CP+  G+  TY NS  +L  YP + L + W L D  G D  C   PA I
Sbjct:   31 SKALAAGDVSISNCPKSKCILKRNTEASIQMKIRPERDFQELTSDIQGII--LDVPLPFPGYYGTSACPHIYDEAGEKKVGCPLKAGQVYTYKNSFKILPVYPTVSLEIHWGLGDKHG-DAACFQIPAKI 157

[Close]

BLAST of ML domain-containing protein vs. Ensembl Fly
Match: Npc2e (gene:FBgn0051410 transcript:FBtr0273369)

HSP 1 Score: 52.7582 bits (125), Expect = 8.248e-9
Identity = 37/117 (31.62%), Postives = 54/117 (46.15%), Query Frame = 1

Query:  175 VDITPCNEQPCQLKKGTTPQLTIKYKALEDT-KSGTVSVHGKIGPIFVPFPLPD--SDLCK--FSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            V+I  C E PC + KGT   + + +    +  KS T +   K+  + +P+ LPD  SD+C+       CPI   E+VTY  +  V  S+P I   V   L D Q   + C      I
Sbjct:   32 VNIKDCEEPPCVVYKGTIAVMEVHFLGNNNNIKSITATTTAKVLGMNLPYALPDEVSDVCRNLLYGAICPIDKDEDVTYQFNFYVEPSFPEITADVTVTLNDAQNEPITCFVVSCKI 148

[Close]

BLAST of ML domain-containing protein vs. Ensembl Fly
Match: Npc2c (gene:FBgn0037783 transcript:FBtr0082176)

HSP 1 Score: 46.595 bits (109), Expect = 1.747e-6
Identity = 34/113 (30.09%), Postives = 55/113 (48.67%), Query Frame = 1

Query:  175 VDITPCNEQPCQLKKGTTPQLTIKYKALEDT-KSGTVSVHGKIGPIFVPFPLPDS--DLCK--FSSPACPITTGEEVTYSNSITVLSSYPAI--RLTVKWELVDDQGNDLICI 492
            V I  C+  PC L KGT  ++ I++ A  +T K  +  VH     + +P+ L  S  ++C        CP+  GE+VTY   + V ++ P +  RL V+    DD+   + C 
Sbjct:   40 VQIDDCDALPCDLWKGTEAKIDIQFVATRNTMKKLSAEVHLTSLGVTIPYDLEASRGNVCSNLLHGAYCPLDAGEDVTYQLLLPVTTNQPEVPTRLEVRLLDSDDENRVVSCF 152

[Close]

BLAST of ML domain-containing protein vs. Ensembl Zebrafish
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:282673])

HSP 1 Score: 114.005 bits (284), Expect = 2.846e-32
Identity = 56/132 (42.42%), Postives = 79/132 (59.85%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  ++DCGS  GKV  VDI PC++QPC+L KG +  + + + +  ++++    VHG +  + VPFP+P  D CK S   CPI   +   Y   + V + YPAI++ V+WEL DD   DL CI FP  IVN
Sbjct:   19 ADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTSKAVVHGVLAGVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFPVQIVN 149

[Close]

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  ++DCGS  GKV  VDI PC++QPC+L KG +  + + + +  ++++    VHG +  + VPFP+P  D CK S   CPI   +   Y   + V + YPAI++ V+WEL DD   DL CI FP  IVN
Sbjct:   19 ADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTSKAVVHGVLAGVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFPVQIVN 149

[Close]

BLAST of ML domain-containing protein vs. Ensembl Zebrafish
Match: zgc:193725 (zgc:193725 [Source:ZFIN;Acc:ZDB-GENE-080723-11])

HSP 1 Score: 113.62 bits (283), Expect = 4.683e-32
Identity = 57/132 (43.18%), Postives = 78/132 (59.09%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  ++DCGS  GKV  VDI PC++QPCQL KG +  + + + +   +++    VHG +  + VPFP+P  D CK S   CPI   +   Y   + V + YPAI++ V+WEL DD   DL CI FP  IVN
Sbjct:   19 ADPVKFVDCGSVHGKVVQVDIKPCSQQPCQLHKGQSYTVNVTFISSVASQTSKAVVHGVVECVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFPVQIVN 149

[Close]

BLAST of ML domain-containing protein vs. Ensembl Xenopus
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:493351])

HSP 1 Score: 108.612 bits (270), Expect = 6.487e-30
Identity = 53/128 (41.41%), Postives = 78/128 (60.94%), Query Frame = 1

Query:  127 PTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            P  Y DCGS+ GK+ ++D++PC E+PC L +G+T  +   + +  ++KS +  VHG I  I VPFP+ + D CK S  +CPI +G+  TY   + + S YP I+L VKW+L D+   DL C   P  I
Sbjct:   27 PLKYKDCGSQSGKLVTLDVSPCPEEPCPLVRGSTYTVNATFVSNVNSKSASAVVHGIIAGIAVPFPISEPDGCK-SGISCPINSGQTYTYVTKLPIKSEYPCIKLVVKWQLQDENNKDLFCWLIPVHI 153

[Close]

BLAST of ML domain-containing protein vs. Ensembl Mouse
Match: Npc2 (NPC intracellular cholesterol transporter 2 [Source:MGI Symbol;Acc:MGI:1915213])

HSP 1 Score: 105.145 bits (261), Expect = 8.079e-29
Identity = 52/131 (39.69%), Postives = 78/131 (59.54%), Query Frame = 1

Query:  118 SAAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
             A P  + DCGSK G ++ V+++PC   PCQL KG +  + I + +   +++ T  VHG +  I VPFP+P+ D CK S   CPI   +  +Y N + V + YP+I+L V+W+L DD+ N+L C   P  I
Sbjct:   18 QAEPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTSGTQSQNSTALVHGILEGIRVPFPIPEPDGCK-SGINCPIQKDKVYSYLNKLPVKNEYPSIKLVVEWKLEDDKKNNLFCWEIPVQI 147

[Close]

BLAST of ML domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9DGJ3|NPC2_DANRE (NPC intracellular cholesterol transporter 2 OS=Danio rerio OX=7955 GN=npc2 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 2.148e-31
Identity = 56/132 (42.42%), Postives = 79/132 (59.85%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  ++DCGS  GKV  VDI PC++QPC+L KG +  + + + +  ++++    VHG +  + VPFP+P  D CK S   CPI   +   Y   + V + YPAI++ V+WEL DD   DL CI FP  IVN
Sbjct:   19 ADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTSKAVVHGVLAGVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFPVQIVN 149

[Close]

BLAST of ML domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9Z0J0|NPC2_MOUSE (NPC intracellular cholesterol transporter 2 OS=Mus musculus OX=10090 GN=Npc2 PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 5.651e-28
Identity = 52/131 (39.69%), Postives = 78/131 (59.54%), Query Frame = 1

Query:  118 SAAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
             A P  + DCGSK G ++ V+++PC   PCQL KG +  + I + +   +++ T  VHG +  I VPFP+P+ D CK S   CPI   +  +Y N + V + YP+I+L V+W+L DD+ N+L C   P  I
Sbjct:   18 QAEPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTSGTQSQNSTALVHGILEGIRVPFPIPEPDGCK-SGINCPIQKDKVYSYLNKLPVKNEYPSIKLVVEWKLEDDKKNNLFCWEIPVQI 147

[Close]

BLAST of ML domain-containing protein vs. UniProt/SwissProt
Match: sp|P61917|NPC2_PANTR (NPC intracellular cholesterol transporter 2 OS=Pan troglodytes OX=9598 GN=NPC2 PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 7.409e-28
Identity = 52/132 (39.39%), Postives = 77/132 (58.33%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  IV+
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVS 149

[Close]

BLAST of ML domain-containing protein vs. UniProt/SwissProt
Match: sp|P61918|NPC2_MACFA (NPC intracellular cholesterol transporter 2 OS=Macaca fascicularis OX=9541 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 7.409e-28
Identity = 52/132 (39.39%), Postives = 77/132 (58.33%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  IV+
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVS 149

[Close]

BLAST of ML domain-containing protein vs. UniProt/SwissProt
Match: sp|P61916|NPC2_HUMAN (NPC intracellular cholesterol transporter 2 OS=Homo sapiens OX=9606 GN=NPC2 PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 7.409e-28
Identity = 52/132 (39.39%), Postives = 77/132 (58.33%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            A P  + DCGS  G ++ V+++PC  QPCQL KG +  + + + +   +KS    VHG +  + VPFP+P+ D CK S   CPI   +  +Y N + V S YP+I+L V+W+L DD+   L C   P  IV+
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVS 149

[Close]

BLAST of ML domain-containing protein vs. TrEMBL
Match: A0A3B4TDC2 (Niemann-Pick disease, type C2 OS=Seriola dumerili OX=41447 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.158e-35
Identity = 65/132 (49.24%), Postives = 82/132 (62.12%), Query Frame = 1

Query:  121 AAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            AAP  YLDCGS  GKV  VD+TPCN QPCQL KG +  + + + +  ++++    VHG I  + VPFP+P  D CK S   CPI   +   Y NS+ V S YPAI+L V+WEL DD   DL CI FP  IV+
Sbjct:   19 AAPVKYLDCGSTSGKVAVVDVTPCNTQPCQLHKGQSYSVNVTFNSAVESQTSKAVVHGIIAGVPVPFPIPIEDGCK-SGIQCPIKKQQSYHYLNSLPVKSEYPAIKLVVEWELRDDNKQDLFCIRFPVQIVS 149

[Close]

BLAST of ML domain-containing protein vs. TrEMBL
Match: A0A0X3NXW4 (Epididymal secretory protein E1 OS=Schistocephalus solidus OX=70667 GN=NPC2 PE=4 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 4.129e-35
Identity = 58/123 (47.15%), Postives = 81/123 (65.85%), Query Frame = 1

Query:  142 DCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            DCGSK G   +V++ PC   PC L KG+   LT+ +KA    KSG  SVHG I  + +PFP+PD+DLCKF+SP+CP+  G   TY  S+ V  +YP+I L ++WEL ++ G+  IC+ FPA +
Sbjct:   55 DCGSKSGTFSAVEVVPCETNPCTLYKGSHANLTLTFKADHTVKSGKASVHGIIAGVPIPFPIPDNDLCKFASPSCPLDAGTTYTYMYSLEVKKTYPSISLHIRWELKEEDGDFHICVQFPAKL 177

[Close]

BLAST of ML domain-containing protein vs. TrEMBL
Match: A0A267G469 (ML domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027630g2 PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 8.370e-35
Identity = 57/128 (44.53%), Postives = 86/128 (67.19%), Query Frame = 1

Query:  130 TVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            T ++DCGSK GK++S+D+TPC + PC+L +GT  Q+ I +   E++ S    VHG +  I VPFPLPD++ C   +PACP+  G +  Y++++ V SSYPA+ + VKWEL D +  D +C+  PA +V
Sbjct:   22 TKFVDCGSKAGKIDSLDVTPCPKIPCELPRGTNAQIKITFTPSENSTSVKAVVHGIVAKIPVPFPLPDTNACDSMAPACPLKAGTQAVYTSTLQVKSSYPAVGVVVKWELADAKEVDFVCLEVPARLV 149

[Close]

BLAST of ML domain-containing protein vs. TrEMBL
Match: A0A267EQ39 (ML domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025232g1 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 2.154e-34
Identity = 58/129 (44.96%), Postives = 79/129 (61.24%), Query Frame = 1

Query:  127 PTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            P  + DCGSK GK+ SVDI PC + PC+LK+GT   +T+ + + E   +    VHG I  I VPFPLPD + C   SP CP++ G    Y+N++ V  +YP + L V+WEL D   NDL+C   PA +V
Sbjct:   21 PVKFKDCGSKVGKINSVDINPCPKLPCELKRGTKVGVTVDFTSSESATAVKSVVHGVISGIAVPFPLPDPNACNAMSPKCPLSNGASYKYTNALEVQQAYPTLALVVRWELTDSNNNDLVCFEVPARVV 149

[Close]

BLAST of ML domain-containing protein vs. TrEMBL
Match: A0A1I8I3Y3 (ML domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027630g1 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 2.250e-34
Identity = 56/128 (43.75%), Postives = 86/128 (67.19%), Query Frame = 1

Query:  130 TVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            T ++DCGSK GK++S+D+TPC + PC+L +G+  Q+ I +   E++ S    VHG +  I VPFPLPD++ C   +PACP+  G +  Y++++ V SSYPA+ + VKWEL D +  D +C+  PA +V
Sbjct:   22 TKFVDCGSKAGKIDSLDVTPCPKIPCELPRGSNAQIKITFTPSENSTSVKAVVHGIVAKIPVPFPLPDTNACDSMAPACPLKAGTQAVYTSTLQVKSSYPAVGVVVKWELADAKEVDFVCLEVPARLV 149

[Close]

BLAST of ML domain-containing protein vs. Ensembl Cavefish
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:282673])

HSP 1 Score: 107.842 bits (268), Expect = 4.362e-30
Identity = 53/123 (43.09%), Postives = 75/123 (60.98%), Query Frame = 1

Query:  148 GSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            GS  GKV  VDI+PC  QPC+L KG +  + + + +  D+++    VHG I  + VP+P+P+ D CK S   CPI   +  +Y N + V + YP+I+L V+WEL DD   DL CI FP  IV+
Sbjct:   15 GSVEGKVSQVDISPCPTQPCELHKGKSYTVNVTFSSDVDSQTSKALVHGVIAGVPVPYPIPNEDGCK-SGIQCPIQKQKSYSYINELPVKAEYPSIKLVVEWELRDDSSKDLFCIKFPVQIVS 136

[Close]

BLAST of ML domain-containing protein vs. Ensembl Cavefish
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:282673])

HSP 1 Score: 108.227 bits (269), Expect = 4.520e-30
Identity = 53/123 (43.09%), Postives = 75/123 (60.98%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPA 504
            + DCGS  GKV  VDI+PC  QPC+L KG +  + + +  + D+++    VHG I  + VP+P+P+ D CK S   CPI   +  +Y N + V + YP+I+L V+WEL DD   DL CI FP 
Sbjct:   24 FADCGSVEGKVSQVDISPCPTQPCELHKGKSYTVNVTFSNV-DSQTSKALVHGVIAGVPVPYPIPNEDGCK-SGIQCPIQKQKSYSYINELPVKAEYPSIKLVVEWELRDDSSKDLFCIKFPV 144

[Close]

BLAST of ML domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008656.1 (pep scaffold:Pmarinus_7.0:GL477843:35666:38308:1 gene:ENSPMAG00000007827.1 transcript:ENSPMAT00000008656.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.531 bits (262), Expect = 9.785e-30
Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 1

Query:  148 GSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            GS+ GK+ +V+I PC  QPC L K  T  + + + +  +++  T  VHG +  I +PFP+P++D CK S   CPI   +   Y   + V S YP+I+L VKWEL DD+ NDL C  FP AI
Sbjct:   21 GSQTGKISAVNIIPCPTQPCILCKDKTYNINVTFTSDTESQGSTAVVHGILAGIPIPFPIPNNDGCK-SGITCPIAKNQAYIYDVGLPVKSDYPSIKLVVKWELRDDKANDLFCFEFPVAI 140

[Close]

BLAST of ML domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006312.1 (pep scaffold:Pmarinus_7.0:GL476404:451273:452406:-1 gene:ENSPMAG00000005702.1 transcript:ENSPMAT00000006312.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 9.292e-12
Identity = 33/85 (38.82%), Postives = 40/85 (47.06%), Query Frame = 1

Query:  265 TKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNS-ITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            + + T  VH  +    V F +P SD CK S    P   G  +        V SSYP I+L  KW+L DD G DL C  FP  IV 
Sbjct:    5 SNTATAVVHSIVAGFPVRFSIPQSDGCK-SGLRLPARRGPRLQLRRQGAPVKSSYPNIKLVEKWQLKDDNGKDLFCFKFPVKIVR 88

[Close]

BLAST of ML domain-containing protein vs. Ensembl Nematostella
Match: EDO30774 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZV1])

HSP 1 Score: 106.301 bits (264), Expect = 8.655e-30
Identity = 53/127 (41.73%), Postives = 77/127 (60.63%), Query Frame = 1

Query:  136 YLDC-GSKG-GKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            + DC G KG G++  +DI+PC  QPC L KGTT  + I +       SG   VHG I  I VPFPLP++D+CK S   CP+  G +  Y +S+ V + YP+++L V+WE+ D++  D++C   P  I
Sbjct:   20 FKDCSGGKGEGEIVELDISPCPTQPCTLHKGTTVSVNITFVPHVTLDSGKAIVHGVIAGIPVPFPLPNADVCKNSGLKCPLEPGTKYVYQSSLEVKTMYPSLKLVVRWEIQDNKNKDVLCFEIPTQI 146

[Close]

BLAST of ML domain-containing protein vs. Ensembl Nematostella
Match: EDO35255 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM29])

HSP 1 Score: 83.1889 bits (204), Expect = 1.001e-20
Identity = 45/125 (36.00%), Postives = 66/125 (52.80%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI 510
            + DCGSK G++ SVD+TPC+  PC +K+G      I +   E   S  V  +   G I VP PLP+SD CK     CP+ +G++V       +  ++P   LT+K EL D   + +IC   P  +
Sbjct:   24 FQDCGSKKGELISVDLTPCSSDPCVIKRGANASGVITFIPHEVVTSSKVLAYAIFGLIPVPLPLPNSDGCKGYGLTCPLKSGKQVELVFEHYIDQTFPTGHLTLKAELKDQDSDVVICGKIPMTL 148

[Close]

BLAST of ML domain-containing protein vs. Ensembl Nematostella
Match: EDO43439 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYC7])

HSP 1 Score: 76.2554 bits (186), Expect = 2.498e-18
Identity = 37/117 (31.62%), Postives = 66/117 (56.41%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLI 486
            Y DCGS+ G++  +DI+PC+ +PC LK+GT+   ++ +   ED K   +S H  I  + +P P+P SD C+    +CP+ +G +  +     + S +P   LT+K  + D   + ++
Sbjct:   12 YRDCGSQEGEIVGMDISPCDSEPCVLKRGTSVDGSLTFIPHEDLKRAKLSAHAIIDKLPLPLPIP-SDACQGYGLSCPVDSGVKSMFKIHQAIESEFPVGNLTLKAAVTDSDTSQVV 127

[Close]

BLAST of ML domain-containing protein vs. Ensembl Medaka
Match: npc2 (NPC intracellular cholesterol transporter 2 [Source:NCBI gene;Acc:111946828])

HSP 1 Score: 121.324 bits (303), Expect = 3.745e-35
Identity = 57/126 (45.24%), Postives = 78/126 (61.90%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            ++DCGS  GKV  VDI PC  QPCQL +G +  + + + +  ++++ T  VHG I  + +PFP+P+ D CK S   CPI  G+   Y+  + V S YP I+L V+WEL DD  NDL CI FP  IV
Sbjct:   25 FVDCGSSSGKVAMVDINPCTAQPCQLHRGDSYTVNVTFNSDVESQTSTAVVHGIIAGVPIPFPIPEPDGCK-SGIQCPIHKGQAYNYATQLPVKSEYPPIKLVVEWELKDDNKNDLFCIRFPVQIV 149

[Close]

BLAST of ML domain-containing protein vs. Planmine SMEST
Match: SMESG000031788.1 (SMESG000031788.1)

HSP 1 Score: 271.937 bits (694), Expect = 1.110e-94
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 1

Query:  118 SAAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            SAAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN
Sbjct:   16 SAAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 148

[Close]

BLAST of ML domain-containing protein vs. Planmine SMEST
Match: SMESG000031800.1 (SMESG000031800.1)

HSP 1 Score: 186.422 bits (472), Expect = 5.127e-61
Identity = 84/130 (64.62%), Postives = 105/130 (80.77%), Query Frame = 1

Query:  127 PTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIVN 516
            PTVYLDCGSK G+V+SV+I+PCN  PCQL KGT P +TIKYK+ ED  SG VSVHGK+G   VPFPLPD+DLCKFS+P CPI +   VTY+N+I +   YP++R+T+ WELVD QGND++C+ FPAAIV+
Sbjct:   19 PTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDVSSGKVSVHGKVGIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAAIVS 148

[Close]

BLAST of ML domain-containing protein vs. Planmine SMEST
Match: SMESG000023323.1 (SMESG000023323.1)

HSP 1 Score: 125.176 bits (313), Expect = 9.992e-37
Identity = 54/128 (42.19%), Postives = 82/128 (64.06%), Query Frame = 1

Query:  130 TVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAIV 513
            T + DCGS  G +++V + PCN +PC +++G    ++I + + ED  SG   VH     I +PFPLP+ DLC+F +P CPI TG +  YS S+ +   YP+IRLT++WEL D    DL+C+ FP +++
Sbjct:   25 THFKDCGSIKGSIKTVSVDPCNSEPCTIQRGQNSTISISFVSKEDCTSGKAKVHALKDGIPIPFPLPNPDLCQFLNPKCPIKTGSDYKYSYSLFIKKIYPSIRLTLRWELQDSLEKDLVCVEFPISVI 152

[Close]

BLAST of ML domain-containing protein vs. Planmine SMEST
Match: SMESG000031796.1 (SMESG000031796.1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.581e-16
Identity = 32/120 (26.67%), Postives = 66/120 (55.00%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYP-AIRLTVKWELVDDQGNDLICI 492
            + +C + G  ++SV++ PC+++ C+L   T  Q+ +K+ A  +   G V++     P     PL + +LC  S   CPI  G +VT+++  T L  +P  +R+ ++ +  D++  ++ C+
Sbjct:   21 FFECTNNGHVIQSVEVDPCSDRQCKLLSQTEMQIKVKFTANRNINFGKVALTVTALPSIFAMPLKEDNLCLSSDSKCPIAKGADVTHTSKYTFLGGFPDGVRVIMQLKANDEKNKEIYCM 140

[Close]

BLAST of ML domain-containing protein vs. Planmine SMEST
Match: SMESG000031795.1 (SMESG000031795.1)

HSP 1 Score: 68.1662 bits (165), Expect = 8.775e-15
Identity = 31/120 (25.83%), Postives = 66/120 (55.00%), Query Frame = 1

Query:  136 YLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYP-AIRLTVKWELVDDQGNDLICI 492
            + +C   G  ++SV++ PC+++ C+L   T  Q+ +K+ A  +   G V++     P     PL + +LC  S   CPI  G +VT+++  T L  +P  +++ ++ ++ D++  ++ C+
Sbjct:   21 FFECTDSGPVIQSVEVDPCSDRQCKLLSQTEMQIKVKFIANRNINLGKVALTVTALPRIFAMPLKEDNLCLSSDSKCPIAKGADVTHTSKYTFLGGFPDGVQVNMQLKVNDEKNKEIYCM 140

The following BLAST results are available for this feature:

BLAST of ML domain-containing protein vs. Ensembl Human
BLAST of ML domain-containing protein vs. Ensembl Celegans
BLAST of ML domain-containing protein vs. Ensembl Fly
BLAST of ML domain-containing protein vs. Ensembl Zebrafish
BLAST of ML domain-containing protein vs. Ensembl Xenopus
BLAST of ML domain-containing protein vs. Ensembl Mouse
BLAST of ML domain-containing protein vs. UniProt/SwissProt
BLAST of ML domain-containing protein vs. TrEMBL
BLAST of ML domain-containing protein vs. Ensembl Cavefish
BLAST of ML domain-containing protein vs. Ensembl Sea Lamprey
BLAST of ML domain-containing protein vs. Ensembl Yeast
BLAST of ML domain-containing protein vs. Ensembl Nematostella
BLAST of ML domain-containing protein vs. Ensembl Medaka
BLAST of ML domain-containing protein vs. Planmine SMEST

BLAST of ML domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
NPC2	1.543e-28	39.39	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	2.623e-28	39.39	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	9.859e-28	39.69	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	1.352e-27	38.93	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	1.797e-27	38.76	NPC intracellular cholesterol transporter 2 [Sourc...	[more]

BLAST of ML domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
heh-1	3.262e-12	33.65	pep chromosome:WBcel235:III:3198477:3199516:-1 gen...	[more]

BLAST of ML domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Npc2a	1.821e-26	40.16	gene:FBgn0031381 transcript:FBtr0077816	[more]
Npc2b	1.353e-10	30.00	gene:FBgn0038198 transcript:FBtr0082888	[more]
Npc2b	1.353e-10	30.00	gene:FBgn0038198 transcript:FBtr0082889	[more]
Npc2e	8.248e-9	31.62	gene:FBgn0051410 transcript:FBtr0273369	[more]
Npc2c	1.747e-6	30.09	gene:FBgn0037783 transcript:FBtr0082176	[more]

BLAST of ML domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
npc2	2.846e-32	42.42	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]
npc2	2.846e-32	42.42	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]
zgc:193725	4.683e-32	43.18	zgc:193725 [Source:ZFIN;Acc:ZDB-GENE-080723-11]	[more]

BLAST of ML domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
npc2	6.487e-30	41.41	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]

BLAST of ML domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Npc2	8.079e-29	39.69	NPC intracellular cholesterol transporter 2 [Sourc...	[more]

BLAST of ML domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9DGJ3\|NPC2_DANRE	2.148e-31	42.42	NPC intracellular cholesterol transporter 2 OS=Dan...	[more]
sp\|Q9Z0J0\|NPC2_MOUSE	5.651e-28	39.69	NPC intracellular cholesterol transporter 2 OS=Mus...	[more]
sp\|P61917\|NPC2_PANTR	7.409e-28	39.39	NPC intracellular cholesterol transporter 2 OS=Pan...	[more]
sp\|P61918\|NPC2_MACFA	7.409e-28	39.39	NPC intracellular cholesterol transporter 2 OS=Mac...	[more]
sp\|P61916\|NPC2_HUMAN	7.409e-28	39.39	NPC intracellular cholesterol transporter 2 OS=Hom...	[more]

BLAST of ML domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3B4TDC2	1.158e-35	49.24	Niemann-Pick disease, type C2 OS=Seriola dumerili ...	[more]
A0A0X3NXW4	4.129e-35	47.15	Epididymal secretory protein E1 OS=Schistocephalus...	[more]
A0A267G469	8.370e-35	44.53	ML domain-containing protein OS=Macrostomum lignan...	[more]
A0A267EQ39	2.154e-34	44.96	ML domain-containing protein OS=Macrostomum lignan...	[more]
A0A1I8I3Y3	2.250e-34	43.75	ML domain-containing protein OS=Macrostomum lignan...	[more]

BLAST of ML domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
npc2	4.362e-30	43.09	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]
npc2	4.520e-30	43.09	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]

BLAST of ML domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000008656.1	9.785e-30	42.98	pep scaffold:Pmarinus_7.0:GL477843:35666:38308:1 g...	[more]
ENSPMAT00000006312.1	9.292e-12	38.82	pep scaffold:Pmarinus_7.0:GL476404:451273:452406:-...	[more]

BLAST of ML domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of ML domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3


Match Name	E-value	Identity	Description
EDO30774	8.655e-30	41.73	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35255	1.001e-20	36.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43439	2.498e-18	31.62	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of ML domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
npc2	3.745e-35	45.24	NPC intracellular cholesterol transporter 2 [Sourc...	[more]

BLAST of ML domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000031788.1	1.110e-94	100.00	SMESG000031788.1	[more]
SMESG000031800.1	5.127e-61	64.62	SMESG000031800.1	[more]
SMESG000023323.1	9.992e-37	42.19	SMESG000023323.1	[more]
SMESG000031796.1	3.581e-16	26.67	SMESG000031796.1	[more]
SMESG000031795.1	8.775e-15	25.83	SMESG000031795.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30019175 ID=SMED30019175|Name=ML domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=543bp
GAAATAAAATGGCATGATTATTCGACTTTGAATTATTTGACATAATTCCA
TTTTAATTGAATGAGAAAAATTCATTTTTCACCAACTATAAATATTTTTA
CAATTTTCAATACCATTTCAGCAGCTCCAACAGTTTATTTGGATTGCGGT
TCAAAAGGTGGAAAGGTCGAATCCGTTGACATTACGCCATGTAATGAGCA
GCCGTGCCAATTGAAAAAAGGAACTACGCCTCAATTGACTATTAAATACA
AAGCATTAGAAGATACAAAATCTGGTACGGTTTCAGTTCATGGAAAAATC
GGTCCGATTTTTGTCCCATTCCCTCTACCAGATAGTGATCTTTGCAAATT
CAGTTCTCCGGCCTGTCCCATCACAACGGGAGAAGAAGTCACCTACTCCA
ATTCAATAACGGTTTTGTCAAGTTATCCAGCAATTCGATTGACCGTTAAA
TGGGAACTTGTGGATGACCAAGGCAACGATTTAATTTGCATAACATTCCC
AGCAGCAATTGTTAATTAAATTCTTGTCAATTTAGAAATAAAA

protein sequence of SMED30019175-orf-1

>SMED30019175-orf-1 ID=SMED30019175-orf-1|Name=SMED30019175-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=153bp
MRKIHFSPTINIFTIFNTISAAPTVYLDCGSKGGKVESVDITPCNEQPCQ
LKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSP
ACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAAI
VN*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000067	goblet cell
PLANA:0000418	head
PLANA:0000419	prepharyngeal region
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR039670	NPC2-like
IPR033916	ML_Npc2-like
IPR003172	ML_dom
IPR014756	Ig_E-set

Vocabulary: **cellular component**
Term	Definition
GO:0005622	intracellular

Vocabulary: **biological process**
Term	Definition
GO:0032367	intracellular cholesterol transport

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003172	MD-2-related lipid-recognition domain	SMART	SM00737	pgtp_13	coord: 26..148 e-value: 1.3E-32 score: 124.4
IPR003172	MD-2-related lipid-recognition domain	PFAM	PF02221	E1_DerP2_DerF2	coord: 28..150 e-value: 1.1E-26 score: 93.8
None	No IPR available	GENE3D	G3DSA:2.60.40.770		coord: 23..152 e-value: 3.8E-45 score: 155.3
None	No IPR available	PANTHER	PTHR11306:SF32	NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 2 HOMOLOG A	coord: 21..151
IPR039670	Sterol transport protein NPC2-like	PANTHER	PTHR11306	NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED	coord: 21..151
IPR033916	Npc2 like, ML domain	CDD	cd00916	Npc2_like	coord: 28..148 e-value: 3.19734E-41 score: 132.047
IPR014756	Immunoglobulin E-set	SUPERFAMILY	SSF81296	E set domains	coord: 26..151

Main menu

ML domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Owlery

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