SMED30019147
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019147 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16Note: Hover over icons to view figure legend
Alignments
SMED30019147 aligns in the following genomic locations:
Homology
BLAST of SMED30019147 vs. Planmine SMEST
Match: SMESG000061850.1 (SMESG000061850.1) HSP 1 Score: 902.508 bits (2331), Expect = 0.000e+0 Identity = 460/463 (99.35%), Postives = 462/463 (99.78%), Query Frame = 2 Query: 482 MRSFTHLDYLYLEINNQKFQKLFMEDKATQSDECKHEQIVTEQNXXXXXXXXXXXXXXSIDDNALLTSQEKSSKIDNLLKLHREIKMKRKFQTLKERTSTMTRNFYKKLTLKKHSKSQSLCEPSTMNGEVVNGTKVTINIDETKNSEIIIPRTVEVNFRSNPMDHDDTQLNGKSAPPKIVKSVSSVIAIEDIISENDSIFTKFDIIPLLNNYLASELRDSAISDFDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISLTSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINASKTTPGQRWICFPIHSINTFENEISLSEKDDFQKTLLKLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKECLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISSTWPLDYLALFMAELVMDSDY 1870 MRSFTHLDYLYLEINNQKFQKLFMEDKATQSDECKHEQIVTEQNRRVPPKRPPPPKVKS+DDNALLTSQEKSSKIDNLLKLHREIKMKRKFQTLK+RTSTMTRNFYKKLTLKKHSKSQSLCEPSTMNGEVVNGTKVTINIDETKNSEIIIPRTVEVNFRSNPMDHDD QLNGKSAPPKIVKSVSSVIAIEDIISENDSIFTKFDIIPLLNNYLASELRDSAISDFDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISLTSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINASKTTPGQRWICFPIHSINTFENEISLSEKDDFQKTLLKLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKECLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISSTWPLDYLALFMAELVMDSDY Sbjct: 1 MRSFTHLDYLYLEINNQKFQKLFMEDKATQSDECKHEQIVTEQNRRVPPKRPPPPKVKSVDDNALLTSQEKSSKIDNLLKLHREIKMKRKFQTLKDRTSTMTRNFYKKLTLKKHSKSQSLCEPSTMNGEVVNGTKVTINIDETKNSEIIIPRTVEVNFRSNPMDHDDIQLNGKSAPPKIVKSVSSVIAIEDIISENDSIFTKFDIIPLLNNYLASELRDSAISDFDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISLTSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINASKTTPGQRWICFPIHSINTFENEISLSEKDDFQKTLLKLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKECLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISSTWPLDYLALFMAELVMDSDY 463
BLAST of SMED30019147 vs. Planmine SMEST
Match: SMESG000061850.1 (SMESG000061850.1) HSP 1 Score: 855.899 bits (2210), Expect = 0.000e+0 Identity = 437/440 (99.32%), Postives = 439/440 (99.77%), Query Frame = 2 Query: 551 MEDKATQSDECKHEQIVTEQNXXXXXXXXXXXXXXSIDDNALLTSQEKSSKIDNLLKLHREIKMKRKFQTLKERTSTMTRNFYKKLTLKKHSKSQSLCEPSTMNGEVVNGTKVTINIDETKNSEIIIPRTVEVNFRSNPMDHDDTQLNGKSAPPKIVKSVSSVIAIEDIISENDSIFTKFDIIPLLNNYLASELRDSAISDFDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISLTSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINASKTTPGQRWICFPIHSINTFENEISLSEKDDFQKTLLKLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKECLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISSTWPLDYLALFMAELVMDSDY 1870 MEDKATQSDECKHEQIVTEQNRRVPPKRPPPPKVKS+DDNALLTSQEKSSKIDNLLKLHREIKMKRKFQTLK+RTSTMTRNFYKKLTLKKHSKSQSLCEPSTMNGEVVNGTKVTINIDETKNSEIIIPRTVEVNFRSNPMDHDD QLNGKSAPPKIVKSVSSVIAIEDIISENDSIFTKFDIIPLLNNYLASELRDSAISDFDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISLTSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINASKTTPGQRWICFPIHSINTFENEISLSEKDDFQKTLLKLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKECLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISSTWPLDYLALFMAELVMDSDY Sbjct: 1 MEDKATQSDECKHEQIVTEQNRRVPPKRPPPPKVKSVDDNALLTSQEKSSKIDNLLKLHREIKMKRKFQTLKDRTSTMTRNFYKKLTLKKHSKSQSLCEPSTMNGEVVNGTKVTINIDETKNSEIIIPRTVEVNFRSNPMDHDDIQLNGKSAPPKIVKSVSSVIAIEDIISENDSIFTKFDIIPLLNNYLASELRDSAISDFDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISLTSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINASKTTPGQRWICFPIHSINTFENEISLSEKDDFQKTLLKLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKECLNESELFSLEFFGCNSIENISEVLLESKRNTQKLIKDFFQRPEVISILISSTWPLDYLALFMAELVMDSDY 440
BLAST of SMED30019147 vs. Planmine SMEST
Match: SMESG000038056.1 (SMESG000038056.1) HSP 1 Score: 77.0258 bits (188), Expect = 1.943e-14 Identity = 76/282 (26.95%), Postives = 131/282 (46.45%), Query Frame = 2 Query: 1088 FDIIPLLNNYLASELRDSAISD-----FDQYVDSCLTAFIKTCHLQVTDTLNDLNDKLSHFLDLDSISL-TSNQPELYTVHCIPGWKQIRFIEDNNTKEKYISLKNSHFQSWVQINA-----SKTTP----GQRWICFPIHSINTFENEISLSEKDDFQKTLL--KLIMLICINEVYNDEKFPKNIDPNTILLVSSKCGVLVIFKE----CLNESELFSLEFFGCNSIENISEVLLESKRNTQKL---IKDFFQRPEVISILISSTWPLDYLALFMAELVMD 1861 FDI ++ ++EL + + + F +++ +C+ +F+ +Q+ +L +N + +DL S +L N+ + Y + I G K I + K+K+I+LK ++WV + + TP Q WICFP SI E + + LL LI LI I N ++P+ IL+VSS V+ +F E C + E L + + E+ L +++ KL ++ F PE S LI++++PLDYL + +L D Sbjct: 273 FDICSTVSYLFSNELEEMSFAKSDEKMFQKFMTNCVNSFLDVHKIQIGTSLEMINRNIGEHVDLYSPNLDVINKSKKYQITEIFGLKGTCIITNTGNKKKFIALKQG-VKNWVLFTSFLQHIEQDTPLPIHHQEWICFPAQSITNLYIEKNFFNPLHVECKLLLDSLIQLIVILYCQNIHNIHIKLNPSNILIVSSNLSVIFVFIESDAGCASTDESQWLNLYDYSD----KEMRLSYQQSVMKLNKYVEKFLSLPENASHLITASYPLDYLFYLLLKLNKD 549 The following BLAST results are available for this feature:
BLAST of SMED30019147 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30019147 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30019147 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30019147 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30019147 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019147 ID=SMED30019147|Name=SMED30019147|organism=Schmidtea mediterranea sexual|type=transcript|length=1979bpback to top protein sequence of SMED30019147-orf-1 >SMED30019147-orf-1 ID=SMED30019147-orf-1|Name=SMED30019147-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=464bp MRSFTHLDYLYLEINNQKFQKLFMEDKATQSDECKHEQIVTEQNRRVPPKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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