SMED30019133

Overview
NameSMED30019133
Smed IDSMED30019133
Length (bp)1527
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019133 (SMED30019133) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019133 (SMED30019133) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019133 (SMED30019133) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019133 (SMED30019133) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019133

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30019133h1SMcG0000023 Contig3751GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30019133h1SMcG0003947 Contig3751GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30019133h1SMcG0003947 dd_Smed_v4_6722_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019133h1SMcG0003947 dd_Smed_v4_6722_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30019133h1SMcG0003947 dd_Smed_v4_6722_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019133 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005141:1096..2618 +6210
Homology
[Close]
BLAST of SMED30019133 vs. TrEMBL
Match: A0A4S2LSU4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006639 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.813e-14
Identity = 80/305 (26.23%), Postives = 146/305 (47.87%), Query Frame = 2 
Query:   62 LDDLIPINEALLLDKSKFYKIIKEFMPHSSYC--GSMLIPEKMTDWCSYLQCDGIT--CKNEIISEALSKPILPSIVQNLCNQNITR--DPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLP 958
            LD+L  + EAL L    + K  K  + +  YC  G      K+ +WC +L C G+T  C +   +  +S  +  +  +   N ++ +  + + S + +C+D Y C     S+  HYVLG     L +   + ++V L  ++    +    T+ L    + D+ YL   ++ ++S Y   S+F +Q+  Y+ F    V    P   + E +  W+++ L+ +R  YL  G  +K L +       V ++    VAFS  K   Y LE+   ST+  R+ ++ +G +  ++NL+  WLK+P++  LP
Sbjct:    7 LDELPTVEEALKLPYDDYMKYAKGVLEYRDYCFDGFAEAITKVHEWCIHLNCSGVTRTCASLETTTTVSAFVTTATGELTSNASVFQLIESLRSNDSNCYDDYLCDHPGMSQANHYVLGFACFFLCLGGIVSNLVLLPAYNLGLNRSGA-TVYLTAMAVLDLLYLFLALVMVVSRY-MTSDFQEQMVSYARFSARLVPVGLPLMQMCELVVVWLVVALLANRLMYLKFGCQAKLLCSQLQAFKSVAALIAFGVAFSGCKFLEYRLEEFN-STDVVRVIFTPVGGTSLYKNLMYHWLKVPLQMFLP 308
[Close]
BLAST of SMED30019133 vs. TrEMBL
Match: A0A1S8WJQ2 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_09531 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 9.499e-14
Identity = 82/321 (25.55%), Postives = 153/321 (47.66%), Query Frame = 2 
Query:   62 LDDLIPINEALLLDKSKFYKIIKEFMPHSSYC--GSMLIPEKMTDWCSYLQCDGIT--CKNEIISEALSKPILPSIVQNLCNQNITR--DPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMVCHKLI 1006
            LD+L  + EAL L    + K  K  + +  YC  G      K+ +WC +L C G+T  C +   +  ++  +  ++     N ++ +  + + S +  C+D Y C     S+  HYVLG     L +   + ++V L  ++    +    T+ L    + D+ YL   ++ ++S Y   S+F +Q+  Y+ F    V    P   + E +  W+++ L+ +R  YL  G  +K L +       V ++    VA+S  K   Y LE+   ST+  R+ ++ +G +  ++NL+  WLK+P++  LP   V + I +V  K++
Sbjct:    7 LDELPTVEEALKLPYDDYMKYAKGVLEYRDYCFDGFAEAITKVHEWCIHLNCSGVTRTCASLETTTTVTTFVTTAMEGLTPNASVFQLIESLRSNHSKCYDDYLCDHPGMSQANHYVLGFACFFLCLGGIVSNLVLLPAYNLGLNRSGA-TVYLTAMAVLDLLYLFLALVMVVSRY-MTSDFQEQMVSYARFSARLVPVGLPLMQMCELVVVWLVVALLANRLMYLKFGCQAKLLCSQLQAFKSVAALIAFGVAYSGCKFLEYRLEEFN-STDVVRVVFTPVGGTSLYKNLMYHWLKVPLQMFLPYLIVGVLISVVVSKML 324
[Close]
BLAST of SMED30019133 vs. TrEMBL
Match: A0A504YFJ7 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_10830 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 3.232e-13
Identity = 83/344 (24.13%), Postives = 157/344 (45.64%), Query Frame = 2 
Query:   59 SLDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSMLIP--EKMTDWCSYLQCDGIT----------CKNEII---SEALSKPILPSIVQNLC----NQNITRD--------PILSYNGS-CFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMVCHKLI 1006
            +LD+L  + EAL +    + K  +  +    YC   L    EK+ +WC +L+C   +            +E++   S A++ P     ++N      N +I            +  YN S C+D Y C  V  S   H +LG    +L ++  + ++V L  ++    +    T+ L   +I DI Y+  T++ + S Y   ++F+ Q++ Y+ F   FV    P     E +  W+ + L+ +R  YL  G  SK L +       + ++ +  +A++  K+  Y L K       +R+  + IG +  ++NL+  WLK+P++  LP  +V + + +V  K++
Sbjct:    6 NLDELPTVTEALKMPYQTYLKHARGVLEFKEYCQMGLTESLEKVHEWCVHLKCLSASDPCIPSRTNDTADELVFLESSAMTTPYPTDSIRNASFLDSNSSIFDKNHSVLNFMKVFGYNKSKCYDDYLCDQVEMSGPNHGILGFTCFILCLYGIVSNLVLLPAYNIGLNRSGA-TIYLSAMSIFDIAYMALTLLVVTSRY-MPADFSSQMETYAQFSARFVPIGAPIMLFCELVVVWLTVALLANRLLYLKFGFQSKYLCSQLQSVKSITALVLFGLAYASCKMLEYTLIKND-HMNVFRVVLTPIGNTSLYKNLMYHWLKVPLQMFLPYLSVGLLVSLVVTKML 346
[Close]
BLAST of SMED30019133 vs. TrEMBL
Match: G7YPQ3 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_106144 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 8.828e-13
Identity = 79/305 (25.90%), Postives = 146/305 (47.87%), Query Frame = 2 
Query:   62 LDDLIPINEALLLDKSKFYKIIKEFMPHSSYC--GSMLIPEKMTDWCSYLQCDGIT--CKNEIISEALSKPILPSIVQNLCNQNITR--DPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLP 958
            LD+L  + EAL L   ++ K  K  + +  YC  G      K+ +WC +L C G+T  C +   +  +S  +  +  +   N ++ +  + + S +  C+D Y C     S+  HYVLG     L +   + ++V L  ++    +    T+ L    + D+ YL   ++ ++S Y   S+F +Q+  Y+ F    V    P   + E +  W+++ L+ +R  YL  G  +K L +       V ++    VA+S  K   Y LE+   ST+  R+ ++ +G +  ++NL+  WLK+P++  LP
Sbjct:    7 LDELPTVEEALKLPYDEYLKYAKGVLEYRDYCFDGFAEAITKVHEWCIHLNCSGVTRTCASLETTTTVSTFVTTAAEELTSNASVFQLIESLRSNDSKCYDDYLCDHPGMSQANHYVLGFACFFLCLGGIVSNLVLLPAYNLGLNRSGA-TVYLTAMAVLDLLYLSLALVMVVSRY-MTSDFQEQMVSYARFSARLVPVGLPLMQMCELVVVWLVVALLANRLMYLKFGCQAKLLCSQLQAFKSVAALIAFGVAYSGCKFLEYRLEEFN-STDVVRVVFTPVGGTSLYKNLMYHWLKVPLQMFLP 308
[Close]
BLAST of SMED30019133 vs. TrEMBL
Match: A0A0X3PM89 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=TR143602 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.425e-12
Identity = 66/287 (23.00%), Postives = 139/287 (48.43%), Query Frame = 2 
Query:  176 EKMTDWCSYLQCDGITC-----KNEIISEALSKPILPSIVQNLCNQNITRDPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMVCHKLI-FASS 1018
            +K+ +WC+YL C+   C      +E++S   S   L ++  N   +N ++         C+D Y C P   +  ++ + G+F   +   S + ++  +  F++   +    TL      + D  +L   +   L  +  Y  ++ Q+++Y+   +  V  + PF    E  S W+ + ++ DR+ YL  G  S+++ ++ +   +  +V +    ++I K F +EL++LP  ++ YR   + +GKS  F+N++   L++P+E  +P   V +   +   K++ F SS
Sbjct:   47 QKVEEWCAYLNCNSSGCIIPPHPSELVSAVTSLLPLSALSSNASFENASQ--------PCYDDYNCDPPGHAYLFYVIFGLFFTAVCSASLLSNLACVVAFNKASNRTGA-TLYFSAIALADCVHLSVLLFLHLPRF-LYQTYSQQIEVYARTTVRSVPKVLPFLTFSELASVWLTLCVLADRYTYLRLGFFSRSVTSSTLHLRVTSAVLVLTFCYTIPKFFEFELQQLPDKSDVYRAVLTSVGKSGPFRNIMDHLLRVPIEMFIPYIVVGVITCLNVSKMVDFGSS 323
[Close]
BLAST of SMED30019133 vs. Planmine SMEST
Match: SMESG000019555.1 (SMESG000019555.1)

HSP 1 Score: 812.372 bits (2097), Expect = 0.000e+0
Identity = 458/459 (99.78%), Postives = 459/459 (100.00%), Query Frame = 2 
Query:   56 MSLDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSMLIPEKMTDWCSYLQCDGITCKNEIISEALSKPILPSIVQNLCNQNITRDPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMVCHKLIFASSLNSKLNSSEIDFQEFRLTKSILFICCILLLAKVPKMALHILKFEIGIELDMEIKFHLFVEFFDQIYYFIKSCFLYRYMCPKFSSFIQNAFCCERDYKLSKLKHXXXXXXXXXXXXFLYGMKCKEFLFNSNNIDKETIV 1432
            MSLDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSMLIPEKMTDWCSYLQCDGITCKNEIISEALSKPILPSIVQNLCNQNITRDPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDLFESLSFWILILLIFDRFNYLHHGTHSKNLLTNKVCSVLVGSVSISLVAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMVCHKLIFASSLNSKLNSSEIDFQEFRLTKSILFICCILLLAKVPKMALHILKFEIGIELDMEIKFHLFVEFFDQIYYFIKSCFLYRYMCPKFSSFIQNAFCCERDYKLSKLKHDEIECDDEEDKDFLYG+KCKEFLFNSNNIDKETIV
Sbjct:    1 MSLDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSMLIPEKMTDWCSYLQCDGITCKNEIISEALSKPILPSIVQNLCNQNITRDPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDLFESLSFWILILLIFDRFNYLHHGTHSKNLLTNKVCSVLVGSVSISLVAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMVCHKLIFASSLNSKLNSSEIDFQEFRLTKSILFICCILLLAKVPKMALHILKFEIGIELDMEIKFHLFVEFFDQIYYFIKSCFLYRYMCPKFSSFIQNAFCCERDYKLSKLKHDEIECDDEEDKDFLYGIKCKEFLFNSNNIDKETIV 459
[Close]
BLAST of SMED30019133 vs. Planmine SMEST
Match: SMESG000007358.1 (SMESG000007358.1)

HSP 1 Score: 139.428 bits (350), Expect = 6.737e-36
Identity = 126/421 (29.93%), Postives = 210/421 (49.88%), Query Frame = 2 
Query:   62 LDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSMLIPEKMTDWCSYLQCDGITCKNEIISEALSKPILPSIVQNLCNQNITRDP-ILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIVMV----------CHKLIFASSLNSK--------LNSSEIDFQEFRLTKSILFICCILLLAKVPKMALHIL-KFEIGIELDMEIKFHLFVEFFDQIYYFIKSCFLYRYMCPKF 1264
            LD L  ++EAL L KS+F KI K F+ +S+YC   L+P+K+ DWC  L C    C   +  +A           ++ N  IT  P + +    C+D++FC+P W++   +Y +GV   +  + + I +++YL  F   +G++N   +LL +  +C+I  ++FT+      +   S+   QL +YS +   F+ Y+ P    F+++  WIL LL+F+R+ Y  HG H+K + + K   V +    I L  F+++K F   +   P  +  Y + ++ IG +ENF NLI  WLK+P+E   PL      + M+           +KL      N K        +N SE   +E R+  S+L +  + ++ K+PK   HIL   E  +  D      L     D I+ F+K  F+Y Y+ P +
Sbjct:    8 LDKLPFLDEALSLPKSEFNKIAKAFINYSAYCDYFLLPDKVDDWCKKLGCQNGVC-TYLFDDA-----------SVTNAVITTTPKVAALAKHCYDIFFCSPSWSAGINYYSIGVACFVFLVVALIMNIIYLPSFTL-YGQRNGTDILLTSLVLCNIILIIFTIYIDCIPFVQKSH-EQQLYLYSKYVALFIPYILPLQSFFQTMDSWILCLLLFERYLYQQHGKHAKLIFSTKTIIVTILCFIIVLFGFTVVKCFEMTVALKP--SGAYSVHFTDIGMNENFHNLITNWLKVPLEYFGPLLGFIALLSMMYTSLGEQGISMNKLTEKKCANYKKLIKNLVIVNESE---REDRILISVLLMSTMAIVIKLPKFIHHILVNEEANVSFDRLHYIMLSYIVLDAIFIFMKP-FIYIYISPNY 408
[Close]
BLAST of SMED30019133 vs. Planmine SMEST
Match: SMESG000036502.1 (SMESG000036502.1)

HSP 1 Score: 106.301 bits (264), Expect = 3.259e-24
Identity = 129/482 (26.76%), Postives = 212/482 (43.98%), Query Frame = 2 
Query:   59 SLDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSML--IPEKMTDWCSYLQCDGI--TCKNEIISEA---------------LSKPILPSIVQNLCNQNITRDPILSYNGSCFDVYFCAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLLITHTICD-IFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFTDXXXXXXXXXXXXXXXDRFNYLHHGTHSKNLLTNKXXXXXXXXXXXXXXAFSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLLPLTTVFIFIV---------MVCHKLIFASS---LNSKLNSSEID------FQEFR------------------LTKSI--------------LFICCILLLAKVPKMALHILKFEIGIELDMEIKFHLFVEFFDQIYYFIKSCFLYRYMCPKFSSFIQNAFCC 1294
            SLD L    +AL   K  +YK       H + C S    + EK+ +WC  L C G    CK     E                   P++ S    L N       ++ YNG+C+D Y+C     +   HY+LG+  +++ +   + + ++L  F+     +   TL L    I D I+ +LFT+I ++    +   +T QL +YS F  + + +  P   L E LS WI + ++ DRF YL  G HSK + + K  + ++ S+ I L+ ++I K   Y +   P      R+ Y+++G +E+FQN++  WLK+P+E  LP     IF V         M   KL  AS     N++LN+++ +      F EF                   +T S+              L +  +L+  K+PK  LH LK E  ++L     F    +F D  + F+K   ++  +   F   +++ FCC
Sbjct:    8 SLDLLPTYEDALKSSKEAYYKAANGVFEHRTTCLSSFAEVYEKLYEWCVALNCVGSLENCKIPPYPETGVISTSISLISTISIQPHPLVTSSYSQLMN-------VVGYNGTCYDDYYCNQRSVAPYLHYILGITCLIMCLIGILTTSLFLPVFNVA-SHRTGVTLYLTAIAIIDCIYMILFTVIVVIQYIPY--EYTSQLDMYSRFTAHLIPHGLPTLQLMELLSVWITVAVLIDRFLYLKFGFHSKIICSRKASAKIIASILILLLIYTIPKFLEYHVINYP-KLGVLRVMYTEVGFNESFQNMLLHWLKVPIEMFLPYLADAIFSVINSIKFYDLMYKRKLSEASKNKRRNAELNATDENDVKSMMFPEFEKDTAKFPSLTVDSNMPKVITSSLKDAEDKENSMVVMSLILGFLLIALKIPKFILHALKSEQYVDLKSTKFFQPVNDFLDLSWVFLKP-IIFICVAKHFRQVLKSVFCC 477
The following BLAST results are available for this feature:
BLAST of SMED30019133 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LSU41.813e-1426.23Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A1S8WJQ29.499e-1425.55Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A504YFJ73.232e-1324.13Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
G7YPQ38.828e-1325.90Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A0X3PM891.425e-1223.00G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of SMED30019133 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019133 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000019555.10.000e+099.78SMESG000019555.1[more]
SMESG000007358.16.737e-3629.93SMESG000007358.1[more]
SMESG000036502.13.259e-2426.76SMESG000036502.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019133 ID=SMED30019133|Name=SMED30019133|organism=Schmidtea mediterranea sexual|type=transcript|length=1527bp
AAACATACTTAAAAATAATTTATTATAATTAAAGTATATCATTATTCAAA
TTAAGATGTCATTAGACGATTTAATACCAATAAATGAAGCACTTCTTTTG
GATAAATCAAAATTTTATAAAATTATCAAAGAGTTTATGCCTCACTCTTC
TTATTGTGGAAGTATGTTAATTCCAGAAAAAATGACAGATTGGTGTTCTT
ATTTGCAGTGCGATGGCATTACTTGTAAAAATGAAATAATTTCCGAGGCA
TTGAGTAAGCCTATTTTACCATCAATTGTCCAAAATCTTTGCAATCAAAA
CATTACTCGAGATCCGATTCTTTCCTATAATGGGTCATGTTTCGATGTTT
ATTTTTGTGCTCCTGTTTGGGCCAGCAAAGATTATCATTATGTTCTAGGG
GTTTTCGGAATGCTTTTGGCAATTTTCAGTTTTATATTTAGTATGGTTTA
TCTCACAGGATTTCATCGAGAATTTGGAAAGAAAAATACAAGGACTCTTC
TCTTAATCACTCATACAATTTGTGATATTTTTTATCTTCTTTTTACAATG
ATTGGTTTATTATCCATGTATAGTTTTTATTCAAATTTCACTGATCAACT
GAAAATATATTCTTCGTTTCACATCTATTTTGTTAGTTATCTGCAACCAT
TTACTGATCTTTTTGAATCGTTGTCATTTTGGATATTAATTTTACTGATC
TTTGATAGATTCAACTATCTTCATCATGGAACTCATTCCAAAAACTTACT
GACAAATAAAGTGTGCTCAGTTTTAGTTGGATCCGTTTCGATTTCTCTAG
TTGCGTTTTCTATTCTAAAACTCTTTACGTACGAATTAGAAAAATTACCG
ATTTCTACTGAAAATTACCGATTAAAATATTCAAAAATTGGAAAAAGTGA
AAATTTCCAAAACCTTATACGGTTCTGGCTGAAAATGCCCATGGAAAGCC
TTTTACCATTGACAACAGTTTTTATTTTTATAGTCATGGTTTGTCATAAA
TTAATTTTCGCCAGCTCATTGAATTCAAAATTGAATTCTTCGGAAATTGA
TTTTCAGGAGTTCCGATTGACAAAATCAATTTTATTCATTTGTTGTATTC
TTTTGTTGGCAAAAGTGCCAAAAATGGCACTCCACATACTGAAATTCGAG
ATTGGAATCGAATTGGACATGGAGATAAAATTCCATTTGTTTGTAGAGTT
TTTCGATCAAATATATTATTTTATCAAGTCATGTTTCCTATACCGCTACA
TGTGTCCGAAATTCTCAAGTTTTATTCAAAATGCCTTCTGTTGTGAAAGG
GATTATAAATTATCGAAATTAAAACACGACGAAATCGAGTGTGATGATGA
AGAGGATAAAGATTTTCTCTACGGCATGAAATGCAAAGAATTTCTTTTCA
ATTCCAATAATATTGACAAAGAAACTATTGTGTAATGATTTCTAAGCTTA
GAGTTTGAATTACTTTTCTTGTTTATTTCTCTAAACATTGTTTTAAATGT
GCTAAATAAACATTTTTTTAAACAATA
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protein sequence of SMED30019133-orf-1

>SMED30019133-orf-1 ID=SMED30019133-orf-1|Name=SMED30019133-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=460bp
MSLDDLIPINEALLLDKSKFYKIIKEFMPHSSYCGSMLIPEKMTDWCSYL
QCDGITCKNEIISEALSKPILPSIVQNLCNQNITRDPILSYNGSCFDVYF
CAPVWASKDYHYVLGVFGMLLAIFSFIFSMVYLTGFHREFGKKNTRTLLL
ITHTICDIFYLLFTMIGLLSMYSFYSNFTDQLKIYSSFHIYFVSYLQPFT
DLFESLSFWILILLIFDRFNYLHHGTHSKNLLTNKVCSVLVGSVSISLVA
FSILKLFTYELEKLPISTENYRLKYSKIGKSENFQNLIRFWLKMPMESLL
PLTTVFIFIVMVCHKLIFASSLNSKLNSSEIDFQEFRLTKSILFICCILL
LAKVPKMALHILKFEIGIELDMEIKFHLFVEFFDQIYYFIKSCFLYRYMC
PKFSSFIQNAFCCERDYKLSKLKHDEIECDDEEDKDFLYGMKCKEFLFNS
NNIDKETIV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 87..419
e-value: 3.3E-16
score: 61.1
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 112..384
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 112..384
e-value: 3.64766E-11
score: 61.88
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 82..415
NoneNo IPR availableTMHMMTMhelixcoord: 236..258
NoneNo IPR availableTMHMMTMhelixcoord: 194..216
NoneNo IPR availableTMHMMTMhelixcoord: 114..136
NoneNo IPR availableTMHMMTMhelixcoord: 343..362
NoneNo IPR availableTMHMMTMhelixcoord: 149..171
NoneNo IPR availableTMHMMTMhelixcoord: 300..322
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 118..362
score: 10.622