SMED30019073

Overview
NameSMED30019073
Smed IDSMED30019073
Length (bp)2984
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019073 (SMED30019073) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019073 (SMED30019073) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019073 (SMED30019073) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019073 (SMED30019073) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019073

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30019073SMESG000041235.1 SmedASXL_016949SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
cephalic gangliaSMED30019073SMESG000041235.1 dd_Smed_v4_13685_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019073SMESG000041235.1 dd_Smed_v4_13685_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30019073SMESG000041235.1 dd_Smed_v6_13685_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30019073SMESG000041235.1 dd_Smed_v4_13685_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30019073SMESG000041235.1 dd_Smed_v4_21762_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019073SMESG000041235.1 SMED_04891_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019073 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000061:5293..20310 -14465
Homology
BLAST of SMED30019073 vs. Planmine SMEST
Match: SMESG000041235.1 (SMESG000041235.1)

HSP 1 Score: 1701.41 bits (4405), Expect = 0.000e+0
Identity = 933/947 (98.52%), Postives = 941/947 (99.37%), Query Frame = 2
Query:   71 YVHSTEKSLVSKFLFPSFYEIKFFKTHFDLHEEYFNSERTXXXXXXXXXXXXXXASHCKRTLIISSKFLISHFKKYKLIYSSSILGNIMKTWKVHXXXXXXXXXXXXXXXQFKIIIMGGSISTDPDDVTHLVVYKMHEKAHGQIFKTFSDCSNVFNVHYIEFYWKKCSLLISTYDILDNLQETFAVRYFQNCKMHLIGFDEYQLGEMLLCIFDNGGSIYQLDHLHSPDCIFIVPNNSKSENVQRVINEESKDSMKNVVTEDWFWQSVENQYLVKFDDYRVLLSKTIGENTNYLSPPNDVKKSASQVLNDFKTVKLQPSGNPNQNPPFSFSDKEISVDEIKANYNLGFGFYPSQCSKEKCXXXXXXXXXXXXXXVKNIKDKLDVLNNFQAIRTHKLIENSNLLLIFNIFKQITDFFQGFYDIFQPLVINYSIDCCIGMELLPHYNKLKKYFQTYNSKFNQMSDLIDRIDSNAVKEHIQHFVDSNEISSFKYFIEIPKRMLTLIQMQFDDIIKSTSKFHPDIVFFIEISQKNRHVTKLIETLEDKLIEGRNQIIVMKCIGNPAIVQIGNRYRTVVDCMHYCDDGLKCAKSLFLFDNTIVMCNFKASAPGKLFDRVIAHLKEQINSGNNLTRCPSIQSIYRNSPFIEMNIKRKXXXXXXXXXXXXXXRKLEGNACSKFHKLFTGLSKLNWKENPENIYXXXXXXXXXXXXXXXXHRGNFDVQSQMFFTINYIDKQSNLLKNMKLFLVPRVVKHPFKYNKINVALPTDFDWEYNDNKGIKWDEFIVEEKRNFCAIIAQHYEMEFIEDSLLSNSVSSVAFENDWLVSDKYFRTPVKLDKTTTRNLSPSNNSFMGSFKKSPKSMSLKKTITENXXXXXXXXXXXXNTLPSSSSQNYIFEDESQNKETKETLTNHKYPENSPVKSLEETEEFSSLNQSELDRNSPLFSKRILHF 2911
            YVHSTEKSLVSKFLFPSFYEIKFFKTHFDLHEEYFNSERT IFCIDDQFIIDEIASHCKRTLIISSKFLISHFKKYKLIYS+SILGNIMKTWKVHLIGF+DEDDDLIIDLQFKIIIMGGS+STDPDDVTHLVVYKMHEKAHGQIFKTFSDCSNVFNVHYIEFYWKKCSLLISTYDILDNLQETFAVRYFQNCKMHLIGFDEYQLGEMLLCIFDNGGSIYQLDHLHSPDCIFIVPNNSKSENVQRVINEESKDSMKNVVTEDWFWQSVENQYLVKFDDYRVLLSKTIGENTNYLSPPNDVKKSASQVLNDFKTVKLQ SGNPNQNPPFSFSDKEISVDEIKANYNLGFGFYPSQCSKEKCEEYEYRRYNLLLNLVKNIKDKLDVLNNFQAIRTHKLIENSNLLLIFNIFKQITDFFQGFYDIFQPLVINYSIDCCIGMELLPHYN+LKKYFQTYNSKFNQMSDLIDRIDSNAVKEHIQHFVDSNEISSFKYFIEIPKRMLTLIQMQFDDIIKSTSKFHPDIVFFIEISQKNRHVTKLIETLEDKLIEGRNQIIVMKCIGNPAIVQIGNRYRTVVDCMHYCDDGLKCAKSLFLFDNTIVMCNFKASAPGKLFDR+IAHLKEQINSGNNLTRCPSIQSIYRNSPFIEMNIKRK SISSVTTVNSISVRKLEGNACSKFHKLFTGLSKLNWKENPENIYSETEILEKSSLISLISHRGNFDVQSQMFFTINYIDKQSNLLKNMKLFLVPRVVKHPFKYNKINVALPTDFDWEYNDNKGIKWDEFIVEEKRNFCAIIAQHYEMEFIEDSLLSNSVSSVAFENDWLVSDKYFRTPVKLDKTTTRNLSPSNNSFMGSFKKSPKSMSLKKTITENFRRFTRFKKRSKNTLPSSSSQNYIFEDESQNKETK+TLTNHKYPENSPVKSLEETEEFSSLNQS+LDRNSPLFSK  + F
Sbjct:    4 YVHSTEKSLVSKFLFPSFYEIKFFKTHFDLHEEYFNSERTLIFCIDDQFIIDEIASHCKRTLIISSKFLISHFKKYKLIYSNSILGNIMKTWKVHLIGFEDEDDDLIIDLQFKIIIMGGSMSTDPDDVTHLVVYKMHEKAHGQIFKTFSDCSNVFNVHYIEFYWKKCSLLISTYDILDNLQETFAVRYFQNCKMHLIGFDEYQLGEMLLCIFDNGGSIYQLDHLHSPDCIFIVPNNSKSENVQRVINEESKDSMKNVVTEDWFWQSVENQYLVKFDDYRVLLSKTIGENTNYLSPPNDVKKSASQVLNDFKTVKLQTSGNPNQNPPFSFSDKEISVDEIKANYNLGFGFYPSQCSKEKCEEYEYRRYNLLLNLVKNIKDKLDVLNNFQAIRTHKLIENSNLLLIFNIFKQITDFFQGFYDIFQPLVINYSIDCCIGMELLPHYNQLKKYFQTYNSKFNQMSDLIDRIDSNAVKEHIQHFVDSNEISSFKYFIEIPKRMLTLIQMQFDDIIKSTSKFHPDIVFFIEISQKNRHVTKLIETLEDKLIEGRNQIIVMKCIGNPAIVQIGNRYRTVVDCMHYCDDGLKCAKSLFLFDNTIVMCNFKASAPGKLFDRLIAHLKEQINSGNNLTRCPSIQSIYRNSPFIEMNIKRKKSISSVTTVNSISVRKLEGNACSKFHKLFTGLSKLNWKENPENIYSETEILEKSSLISLISHRGNFDVQSQMFFTINYIDKQSNLLKNMKLFLVPRVVKHPFKYNKINVALPTDFDWEYNDNKGIKWDEFIVEEKRNFCAIIAQHYEMEFIEDSLLSNSVSSVAFENDWLVSDKYFRTPVKLDKTTTRNLSPSNNSFMGSFKKSPKSMSLKKTITENFRRFTRFKKRSKNTLPSSSSQNYIFEDESQNKETKKTLTNHKYPENSPVKSLEETEEFSSLNQSQLDRNSPLFSKENIAF 950          
BLAST of SMED30019073 vs. Planmine SMEST
Match: SMESG000041220.1 (SMESG000041220.1)

HSP 1 Score: 166.007 bits (419), Expect = 7.227e-42
Identity = 113/350 (32.29%), Postives = 192/350 (54.86%), Query Frame = 2
Query: 1472 DSNAVKEHIQHFVDSNEISSFKYFIEIPKRMLTLIQMQFDDIIKSTSKFHPDIVFFIEISQKNRHVTKLIETLEDKLIEGRNQIIVMKCIGNPAIVQIGNRYRTVVDCMHYCDDGLKCAKSLFLFDNTIVMCNF-KASAPGKLF--DRVIAHLKEQINSGNNLTRCPSIQSIYRNSPFIEMN----IKRKXXXXXXXXXXXXXXRKLEGNACSKFHKLFTGLSKLNWKENPENIYXXXXXXXXXXXXXXXXHRGNFDVQSQMFFTINYIDKQSNLLKNMKLFLVPRVVKHPFKYNKINVALPTDFDWEYNDNKGIKWDEFIVEEKRNFCAIIAQHYEMEFIEDSLLSNSV 2500
            D+  +KE  +     + +SS K FIEIP+RMLT I++Q  +I++ T   HPDI    E+ +    V+ L+  +       ++QI + K       ++  N Y TVVDCM++  DG    K L + +N++V+ +F     P +L+   +      + +NS N+++         +NS  ++ N    ++R  + S+ +       +++EG  CSK+HKLFTGLS++NWK     ++    +LEKS+++S+ S+   + +  QMFF I+Y D +S  ++     LVPR VK+   Y+K+NV  P +F+W   + K I W E +  EKRNFC  +A H+++E+ E   L+ S+
Sbjct:  300 DNETIKEKFEPLKSKHNVSSIKSFIEIPQRMLTQIELQLKEIVEKTENEHPDIKNLKEMLKITHDVSTLVNQVAQYHAVHKSQITINKYFLKADFLRHDNPYSTVVDCMYFSADGSTLPKMLLVLENSVVISDFVNKIPPNQLYKKVKKNLKKVDTLNSNNSIS--------IKNSLVVDENENSTVQR--TSSNCSASTLNKRKRIEGILCSKYHKLFTGLSEVNWKVENSEMFCTPLLLEKSNIVSVRSNASQWRLYDQMFFAIDYRDPKSQNIEQYTFQLVPRFVKNIQVYDKLNVGFPFEFEWSCPE-KDISWGELVSSEKRNFCYNLACHFDVEYSETYDLTKSL 638          

HSP 2 Score: 67.781 bits (164), Expect = 3.252e-11
Identity = 50/176 (28.41%), Postives = 86/176 (48.86%), Query Frame = 2
Query:  401 QFKIIIMGGSISTDPDD-----VTHLVVYKMHEKAHGQI--FKTFSDCSNVFNVHYIEFYWKKCSLLISTYDILDNLQETFAVRYFQNCKMHLIGFDEYQLGEMLLCIFDNGGSIYQL-DHLHSPDCIFIVPNNSKSENVQRVINEESKDSMKNVVTEDWFWQSVENQYLVKFDDY 904
            QFK++ MGG  + D  D     V  ++ Y+        +   K++   S+ FN+  IE         I+ ++ L +   +F +  F NC++ L+GF+  +   + L IF+N G+   + D  +  + IFI     +SEN    + E S+    + V EDW+W SV   +LV F++Y
Sbjct:  108 QFKVLAMGGIYAVDESDKIFESVNIVIPYRFTRNVQLILNKLKSYRIYSH-FNIDSIE---------INEFEDLHDKSNSFQMGLFNNCRIRLVGFESDKQESISLSIFENNGTFLSISDKNYVEEDIFIFAQGFQSENYAIHLKEISETINLSFVNEDWYWDSVREDFLVPFENY 273          
BLAST of SMED30019073 vs. Planmine SMEST
Match: SMESG000055277.1 (SMESG000055277.1)

HSP 1 Score: 117.472 bits (293), Expect = 1.328e-26
Identity = 107/427 (25.06%), Postives = 198/427 (46.37%), Query Frame = 2
Query: 1220 LNNFQAIRTHKLIENSNLLLIFNIFKQITDFFQGFYDIFQPLVIN-YSIDCCIGMELLPHYNKLKKYFQTYNSKFNQMSDLIDRID--SNAVKEHIQHFVDSNEISSFKYFIEIPKRMLTLIQMQFDDIIKSTSKFHPDIVFFIEISQKNRHVTKLIETLEDKLIEGRNQIIVMKCIGNPAIVQIGNRYRTVVDCMHYCDDGLKCAKSLFLFDNTIVMCNFK---------ASAPGKL---FDRVIAHLKEQINSGNNLTRCPSIQSIYRNSPFIEMNIKRKXXXXXXXXXXXXXXRKLEGNACSKFHKLFTGLSKLNWKENPENIYXXXXXXXXXXXXXXXXHRGNFDVQ-SQMFFTINYIDKQSNLLKNMKLFLVPRVVKHPFKYNKINVALPTDFDWEYNDNKGIKWDEFIVEEKRNFCAIIAQ 2452
            +N FQ +R++ ++  S +  + +IFK     F  F D+ + L+I  +  D C G+E L  +  + +Y+++Y   F  + D ++ I+  SN+VK  ++ F   N       F ++P R   +I     +I+ +T  +HPDI  +  + +K  ++     T+E++ I G  Q +V KC+ +   +   N++ T VDC+ + +       ++F+   TI+ C F              G L     R++   K+ + + NN  + P     +R+S F      ++                   N  SKF+K+F GL+ +NW       Y+E   ++KS    +     + D+  S + F ++YI+  S +   ++L LVP +V     Y  I + +  + +W   ++K + W EFI+ EK NFC    Q
Sbjct:  351 INKFQNLRSNSVL--STVKELDDIFKAFQKIFVFFSDLHKQLIIEEFPEDYCYGIEFLEIFGLVDEYYESYKQLFISLIDRVNFINNYSNSVKTLLKEFCKKNSHPDILSFFKLPIRSWLVINTIITEILTNTRCYHPDIGAWCTVQEKISNLLNYFNTIENERINGVKQNLVAKCLSSIVELNENNQFWTCVDCVLFDETSKLIPLTIFIMKETIMFCAFSDFKKKLCKYEKIKGNLEKSIQRIVRINKDFVPNKNNQEKSP---KKFRSSTFNPEEFVKEFKTIPF------------KNQLSKFNKIFNGLANINWNFVSNKKYNEYFEIKKSQFTKISYECLDNDILISPLSFDLSYINSNS-MNSTIRLQLVPTIVNSIKDYENIKIDINDEIEWTPKESKDMSWIEFILAEKNNFCYFFDQ 759          

HSP 2 Score: 59.6918 bits (143), Expect = 1.090e-8
Identity = 49/184 (26.63%), Postives = 88/184 (47.83%), Query Frame = 2
Query:  401 QFKIIIMGGSISTDPDDVTHLVV-----YKMHEKAHGQIFKTFSDCSN-VFNVHYIEFYWKKCSLLISTYDILDNLQETFAVRYFQNCKMHLIGFDEYQLGEMLLCIFDNGGSIYQLDHL----HSPDCIFIVPNNSKSENVQRVINEE---SKDSMKNVVTEDWFWQSVENQYLVKFDDYRVL 913
            + K ++MG  +S    D T  V+     Y+  EK H       S+C+   F+ + I+++WK  S    T   + N   +F  + F NC+  ++G        ++  I  +GGS+YQL+++    H   C  I   N         +NE    S++  KNVV++DW + SV+  +L+   +Y+++
Sbjct:  112 KVKALLMGAKVSDYITDSTSRVIISKMSYQNQEKLH-------SNCNQRTFSSNLIDYFWKNHSKNPKTNAGIIN---SFRAKLFMNCRFVIVGLVRESFNSVMKAIVKHGGSVYQLENVQIATHVIACREIDIIN---------LNERKFLSENEFKNVVSKDWIFDSVKRGFLMSMINYQII 276          
The following BLAST results are available for this feature:
BLAST of SMED30019073 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019073 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000041235.10.000e+098.52SMESG000041235.1[more]
SMESG000041220.17.227e-4232.29SMESG000041220.1[more]
SMESG000055277.11.328e-2625.06SMESG000055277.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019073 ID=SMED30019073|Name=SMED30019073|organism=Schmidtea mediterranea sexual|type=transcript|length=2984bp
ACTAATTTTAAAAATTTCGACATTTAATTATTAATTGTTAATTAATAAAT
TAATTTAGAACATGACAATTTATGTTCATTCAACAGAAAAAAGTTTAGTA
TCAAAATTTTTATTTCCATCTTTTTACGAAATTAAATTTTTCAAAACACA
TTTTGATCTACATGAAGAATATTTTAATTCTGAAAGAACATTTATCTTTT
GTATTGATGATCAATTCATCATTGATGAAATAGCTTCTCATTGTAAAAGG
ACGCTTATAATTAGCTCAAAATTTTTAATTTCGCATTTCAAAAAATACAA
ACTTATCTATTCTAGTAGTATTTTAGGAAATATAATGAAAACTTGGAAAG
TTCATTTAATTGGATTTGACGATGAGGATGATGATTTAATCATAGATTTA
CAGTTTAAAATTATAATAATGGGTGGTAGTATATCGACTGATCCCGACGA
TGTTACTCACCTAGTTGTGTATAAAATGCACGAAAAAGCACATGGGCAAA
TTTTCAAAACATTTTCTGATTGCTCTAATGTATTTAACGTTCATTATATC
GAATTCTATTGGAAAAAATGCTCATTATTAATAAGTACTTACGATATTTT
AGACAATTTACAAGAAACGTTTGCAGTCAGGTATTTTCAAAACTGTAAGA
TGCATTTAATTGGATTTGATGAATATCAATTGGGGGAAATGCTGCTATGT
ATATTTGATAACGGCGGTAGCATTTATCAACTAGACCATCTCCATTCTCC
CGATTGCATATTCATTGTTCCAAACAATTCGAAATCGGAAAATGTCCAAC
GAGTGATCAACGAGGAGTCAAAAGACTCTATGAAAAATGTCGTTACTGAA
GATTGGTTCTGGCAATCAGTCGAGAATCAATATTTGGTTAAATTTGATGA
TTATCGTGTATTATTGAGCAAGACAATTGGTGAAAATACAAATTATTTAT
CCCCACCGAACGATGTAAAAAAGTCTGCAAGCCAAGTATTAAACGATTTT
AAAACGGTCAAATTACAACCTAGTGGAAACCCCAACCAAAATCCACCATT
TTCATTTTCAGATAAAGAAATCTCCGTTGATGAAATCAAAGCAAATTATA
ATTTGGGTTTCGGTTTTTACCCGTCACAATGCAGCAAGGAAAAATGTGAA
GAATACGAGTACAGGCGTTACAATTTACTCCTTAATCTCGTCAAGAACAT
CAAAGACAAACTCGATGTTTTGAATAATTTCCAAGCCATAAGGACGCACA
AATTAATCGAGAATTCAAATCTATTATTGATATTCAATATTTTCAAGCAG
ATTACAGATTTCTTTCAAGGCTTCTATGATATTTTCCAACCACTCGTAAT
CAATTACTCGATTGACTGTTGCATCGGAATGGAATTATTGCCGCATTATA
ACAAACTGAAAAAGTATTTCCAGACATATAATTCAAAATTCAACCAAATG
AGTGATTTGATCGACAGAATCGACAGCAACGCTGTGAAAGAACACATCCA
ACATTTTGTTGATAGCAATGAAATTTCCTCATTCAAATACTTCATCGAAA
TACCGAAACGAATGCTGACCTTAATCCAAATGCAATTCGATGATATAATA
AAATCAACCTCAAAGTTTCATCCGGATATTGTTTTTTTTATCGAGATCTC
CCAGAAAAATCGACACGTAACGAAACTAATTGAAACATTAGAAGACAAAC
TAATTGAAGGACGTAATCAAATAATTGTGATGAAATGCATTGGAAATCCA
GCAATAGTGCAAATTGGAAATAGATATCGAACTGTTGTAGATTGCATGCA
TTACTGTGATGATGGGTTAAAATGTGCTAAATCTTTATTTCTATTCGATA
ACACAATTGTAATGTGCAATTTTAAAGCATCAGCGCCGGGTAAACTGTTT
GACAGGGTTATTGCTCATTTAAAAGAGCAAATTAACAGTGGAAACAATTT
AACAAGATGTCCCAGTATCCAAAGCATTTATCGTAATAGTCCTTTCATAG
AAATGAACATCAAAAGAAAAAATTCGATAAGTAGCGTAACAACTGTGAAT
TCGATTTCCGTAAGAAAATTGGAAGGAAATGCTTGTTCTAAGTTCCACAA
GCTATTTACTGGGCTGTCGAAATTAAATTGGAAAGAAAACCCAGAAAATA
TTTATTCCGAAACGGAAATTTTAGAAAAATCTAGTTTAATTTCCTTGATA
TCTCACCGAGGTAATTTCGATGTCCAGAGTCAGATGTTTTTCACTATAAA
TTATATCGACAAACAATCAAATCTGTTGAAAAATATGAAATTGTTTCTAG
TACCAAGAGTTGTAAAACACCCGTTCAAATATAACAAAATTAATGTTGCT
TTGCCGACTGATTTTGATTGGGAGTATAATGATAACAAAGGAATAAAATG
GGACGAATTTATTGTTGAAGAAAAACGGAATTTTTGCGCCATTATAGCTC
AGCATTATGAAATGGAATTTATCGAAGACTCACTTTTGAGCAATTCAGTG
TCGTCGGTTGCGTTTGAAAATGATTGGCTTGTCAGTGATAAATATTTCCG
AACTCCTGTGAAACTAGATAAAACCACCACAAGAAATCTGTCTCCTAGTA
ACAACAGTTTTATGGGTTCGTTTAAGAAATCCCCCAAATCTATGTCGCTA
AAAAAGACTATAACAGAAAACTTCAGACGATTTACAAGATTCAAGAAAAG
ATCTAAAAACACTTTGCCCAGTTCTTCTTCACAGAATTATATTTTCGAGG
ACGAGTCTCAGAATAAAGAAACAAAAGAAACCCTAACGAATCACAAATAT
CCAGAAAATTCTCCTGTTAAAAGTTTAGAAGAAACTGAAGAGTTTTCGAG
TTTAAACCAATCAGAACTCGACAGAAATAGTCCATTATTTTCCAAGAGAA
TATTACATTTCCTCCTAATTCAATCTCCAACCAAGAAAAGACCCGAGAAT
CCAGAAAACCAATGAATAATAATAATAATAATAA
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protein sequence of SMED30019073-orf-1

>SMED30019073-orf-1 ID=SMED30019073-orf-1|Name=SMED30019073-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=968bp
MTIYVHSTEKSLVSKFLFPSFYEIKFFKTHFDLHEEYFNSERTFIFCIDD
QFIIDEIASHCKRTLIISSKFLISHFKKYKLIYSSSILGNIMKTWKVHLI
GFDDEDDDLIIDLQFKIIIMGGSISTDPDDVTHLVVYKMHEKAHGQIFKT
FSDCSNVFNVHYIEFYWKKCSLLISTYDILDNLQETFAVRYFQNCKMHLI
GFDEYQLGEMLLCIFDNGGSIYQLDHLHSPDCIFIVPNNSKSENVQRVIN
EESKDSMKNVVTEDWFWQSVENQYLVKFDDYRVLLSKTIGENTNYLSPPN
DVKKSASQVLNDFKTVKLQPSGNPNQNPPFSFSDKEISVDEIKANYNLGF
GFYPSQCSKEKCEEYEYRRYNLLLNLVKNIKDKLDVLNNFQAIRTHKLIE
NSNLLLIFNIFKQITDFFQGFYDIFQPLVINYSIDCCIGMELLPHYNKLK
KYFQTYNSKFNQMSDLIDRIDSNAVKEHIQHFVDSNEISSFKYFIEIPKR
MLTLIQMQFDDIIKSTSKFHPDIVFFIEISQKNRHVTKLIETLEDKLIEG
RNQIIVMKCIGNPAIVQIGNRYRTVVDCMHYCDDGLKCAKSLFLFDNTIV
MCNFKASAPGKLFDRVIAHLKEQINSGNNLTRCPSIQSIYRNSPFIEMNI
KRKNSISSVTTVNSISVRKLEGNACSKFHKLFTGLSKLNWKENPENIYSE
TEILEKSSLISLISHRGNFDVQSQMFFTINYIDKQSNLLKNMKLFLVPRV
VKHPFKYNKINVALPTDFDWEYNDNKGIKWDEFIVEEKRNFCAIIAQHYE
MEFIEDSLLSNSVSSVAFENDWLVSDKYFRTPVKLDKTTTRNLSPSNNSF
MGSFKKSPKSMSLKKTITENFRRFTRFKKRSKNTLPSSSSQNYIFEDESQ
NKETKETLTNHKYPENSPVKSLEETEEFSSLNQSELDRNSPLFSKRILHF
LLIQSPTKKRPENPENQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036420BRCT_dom_sf
IPR035899DBL_dom_sf
IPR001357BRCT_dom
IPR000219DH-domain
Vocabulary: biological process
TermDefinition
GO:0035023regulation of Rho protein signal transduction
GO:0000902cell morphogenesis
GO:0000910cytokinesis
GO:0043547positive regulation of GTPase activity
GO:0045666positive regulation of neuron differentiation
Vocabulary: molecular function
TermDefinition
GO:0005089Rho guanyl-nucleotide exchange factor activity
GO:0005085guanyl-nucleotide exchange factor activity
GO:0005096GTPase activator activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036420BRCT domain superfamilyGENE3DG3DSA:3.40.50.10190coord: 182..285
e-value: 4.6E-9
score: 38.4
IPR036420BRCT domain superfamilySUPERFAMILYSSF52113BRCT domaincoord: 189..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 881..897
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 880..968
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 923..958
NoneNo IPR availableCDDcd17732BRCT_Ect2_rpt2coord: 192..274
e-value: 6.27157E-6
score: 43.0272
IPR001357BRCT domainPROSITEPS50172BRCTcoord: 187..283
score: 11.253
IPR035899Dbl homology (DH) domain superfamilySUPERFAMILYSSF48065DBL homology domain (DH-domain)coord: 390..541