Neutral sphingomyelinase
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019060 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of Neutral sphingomyelinase vs. TrEMBL
Match: A0A504YPF6 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_10893 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 5.265e-13 Identity = 55/148 (37.16%), Postives = 86/148 (58.11%), Query Frame = 1 Query: 118 SNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNEG-KDIAVKIFFML--GLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRETV 552 S + LGL IS L+++ +AT YW+CG+L C G KD+ V + +L GLVS+ +V IL+++G+ + + + AR +L+ +G LLI +LVFT G WSY ++ + +QV I+ +M+S CT R V+V TV Sbjct: 8 STILLGLAIS---LVIVGLATSYWSCGSLFDSCQRGMKDVTVTVIVLLLIGLVSLCIVFILDLVGLCTESLTSSPGYITARFVLIYLGSTCLLIGILVFTGKIGRAWSYFVSTVGCVFAIQVAILAIMSSRCTA---KRERVVVTRTV 149
BLAST of Neutral sphingomyelinase vs. TrEMBL
Match: A0A4E0RN46 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_000078 PE=4 SV=1) HSP 1 Score: 72.7886 bits (177), Expect = 2.039e-12 Identity = 54/148 (36.49%), Postives = 85/148 (57.43%), Query Frame = 1 Query: 118 SNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNEG-KDIAVKIFFML--GLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRETV 552 S + LGL IS L+++ +AT YW+CG+L C G KD+ V + +L GLV + +V IL+++G+ + + + AR +L+ +G LLI +LVFT G WSY ++ + +QV I+ +M+S CT R V+V TV Sbjct: 8 STILLGLAIS---LVIVGLATSYWSCGSLFDSCQRGMKDVTVTVIVLLLIGLVCLCIVFILDLVGLCTESLASSPGYITARFVLIYLGSTCLLIGILVFTGKIGRAWSYFVSTVGCVFAIQVAILAIMSSRCTA---KRERVVVTRTV 149
BLAST of Neutral sphingomyelinase vs. TrEMBL
Match: A0A5J4NS07 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0004886 PE=4 SV=1) HSP 1 Score: 69.3218 bits (168), Expect = 4.470e-11 Identity = 50/134 (37.31%), Postives = 78/134 (58.21%), Query Frame = 1 Query: 130 LGLMISTFILLVIAIATPYWNCGNLLTDCNE--GKDIAVKIFFML--GLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTR 519 LGL I L+ + +AT YW CGNL C KD+ + I +L GL+ +++V +L+++G+ S + ++ AR ILL +G LLI VLV+T GH WSY LTV+ +Q+ I+ +++S C T+ Sbjct: 12 LGLAIG---LVAVGLATSYWRCGNLFEGCQRYGAKDVVIAIIALLLVGLICLSIVFLLDLIGLCSDVFVVSAGYLTARFILLYLGTACLLIGVLVYTGKLGHEWSYFLTVVGCVFAMQIAILAIISSRCVTGTQ 142
BLAST of Neutral sphingomyelinase vs. TrEMBL
Match: H2KRD2 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_106005 PE=4 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 4.961e-11 Identity = 54/144 (37.50%), Postives = 83/144 (57.64%), Query Frame = 1 Query: 106 EMNTSNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNEG--KD--IAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSR 525 + L++ L+ + L+V +AT +W CG+L C KD IAV I ++G+ IA+V +L+++ + S R S ARL+LL IG +LLI +LVFT G+ WSY + LG+QV I+ +++S C+ TTR R Sbjct: 2 AIELHRLAIVLLSFSVALVVCGLATSHWGCGSLFVSCQNADYKDAVIAVIILLLIGVACIAIVFLLDLISLCSDELRSKSGYITARLVLLYIGSAALLIGILVFTGKVGYAWSYFAATVGCVLGIQVAILAVISSRCS-TTRQR 144
BLAST of Neutral sphingomyelinase vs. TrEMBL
Match: A0A4S2LLK5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007720 PE=4 SV=1) HSP 1 Score: 67.781 bits (164), Expect = 1.338e-10 Identity = 56/147 (38.10%), Postives = 83/147 (56.46%), Query Frame = 1 Query: 118 SNLSLGLMISTFILLVIAIATPYWNCGNLLTDCN-EGKD--IAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRET 549 S + LGL IS L++I IAT +W CG+L C KD IAV + ++G V +AVV IL+++G+ S + ++ R +LL +G LLI +LVFT + WSY L + +QV I+ +M+S C T+ V+VR T Sbjct: 8 STILLGLAIS---LVIIGIATNHWGCGSLFEGCQYRDKDATIAVLVLLLIGAVCLAVVFILDLIGLCSDVFVVSAGYLTTRFVLLYLGSAFLLIGILVFTGKKMFAWSYFLATVGTVFAMQVAILAIMSSRCVTGTQ---RVVVRTT 148
BLAST of Neutral sphingomyelinase vs. Planmine SMEST
Match: SMESG000012166.1 (SMESG000012166.1) HSP 1 Score: 255.373 bits (651), Expect = 2.299e-87 Identity = 150/151 (99.34%), Postives = 151/151 (100.00%), Query Frame = 1 Query: 109 MNTSNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNEGKDIAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRETVVTH 561 MNTSNLSLGL+ISTFILLVIAIATPYWNCGNLLTDCNEGKDIAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARLILLGIGICSLLICVLVFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRETVVTH Sbjct: 1 MNTSNLSLGLIISTFILLVIAIATPYWNCGNLLTDCNEGKDIAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARLILLGIGICSLLICVLVFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRETVVTH 151
BLAST of Neutral sphingomyelinase vs. Planmine SMEST
Match: SMESG000012170.1 (SMESG000012170.1) HSP 1 Score: 142.895 bits (359), Expect = 4.566e-43 Identity = 85/133 (63.91%), Postives = 107/133 (80.45%), Query Frame = 1 Query: 109 MNTSNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNE-GKDIAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSC 504 MNT +LSLGL+IS F+LL+IA+ATPYW+CGN+L DCN G +A KIFFMLGLV AVVLILEILG + YR + ++NL +LILLGI + SLLIC+L+F S+ + WSYML V+ +TLGLQV IIG+M+SSC Sbjct: 1 MNTLSLSLGLIISMFVLLIIAVATPYWHCGNILLDCNHYGTWVAAKIFFMLGLVCCAVVLILEILGYACLNYRSHGTMNLVKLILLGISVGSLLICILLFNSVTSNQWSYMLAVVGMTLGLQVFIIGLMSSSC 133
BLAST of Neutral sphingomyelinase vs. Planmine SMEST
Match: SMESG000012168.1 (SMESG000012168.1) HSP 1 Score: 114.39 bits (285), Expect = 6.249e-32 Identity = 85/153 (55.56%), Postives = 113/153 (73.86%), Query Frame = 1 Query: 109 MNTSNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNEGKDIAV-KIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRS-RGHVIVRETVVTH 561 MNT+ LSL L+IST LL+ A+ PYW+CGN+L+DCN + V KIFF+LGLV VVL+LE++ +I+Y GN+S+NL RLI+LGI + SLL+C+L++TS + WSYM TVIA+TL +Q +IG+M+SSC V S HVIVR T VTH Sbjct: 1 MNTNRLSLVLVISTVSLLITAMILPYWHCGNILSDCNYDAGLVVAKIFFILGLVCFVVVLVLELIQFCTISYYGNASLNLVRLIMLGIAVGSLLMCILIYTSNIVNQWSYMCTVIAMTLAVQAFVIGIMSSSCFVVKSSVPDHVIVRRTFVTH 153
BLAST of Neutral sphingomyelinase vs. Planmine SMEST
Match: SMESG000011804.1 (SMESG000011804.1) HSP 1 Score: 69.3218 bits (168), Expect = 1.089e-14 Identity = 47/149 (31.54%), Postives = 87/149 (58.39%), Query Frame = 1 Query: 109 MNTSNLSLGLMISTFILLVIAIATPYWNCGNLLTDC---NEGKDIAVKIFFMLGLVSIAVVLILEILGIVSITYRGNSSINLARXXXXXXXXXXXXXXXXXFTSIRGHNWSYMLTVIALTLGLQVLIIGMMTSSCTVTTRSRGHVIVRE 546 +++ ++L + + +LL+ A ATPYW CG L TDC ++ + ++V I F LG+ + VV +L+ + + T N + N RL+LL +G+ ++ V + S R WS+++ ++ TL +QV+++ + T +C TRS +IV+ Sbjct: 2 IDSVRITLVFLFVSIVLLICAAATPYWGCGYLYTDCIQHDKVQGLSVMILFTLGICFLIVVAVLDFVNCCNGTLLLNVTTNQVRLVLLFLGVGCIVSAVCIHASYRAPIWSFLMAIVGGTLLVQVIVLALNTCTCVTRTRSE-RIIVKH 149 The following BLAST results are available for this feature:
BLAST of Neutral sphingomyelinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Neutral sphingomyelinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Neutral sphingomyelinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Neutral sphingomyelinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019060 ID=SMED30019060|Name=Neutral sphingomyelinase|organism=Schmidtea mediterranea sexual|type=transcript|length=687bpback to top protein sequence of SMED30019060-orf-1 >SMED30019060-orf-1 ID=SMED30019060-orf-1|Name=SMED30019060-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp MNTSNLSLGLMISTFILLVIAIATPYWNCGNLLTDCNEGKDIAVKIFFMLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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