SMED30019020

Overview
NameSMED30019020
Smed IDSMED30019020
Length (bp)1404
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019020 (SMED30019020) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019020 (SMED30019020) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019020 (SMED30019020) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019020 (SMED30019020) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30019020SMESG000015266.1 SmedASXL_011582SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
headSMED30019020SMESG000015266.1 dd_Smed_v6_14297_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
headSMED30019020SMESG000073636.1 SMESG000015266.1 SMESG000071518.1 OX_Smed_1.0.02551ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019020 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.019028:2184..3586 +6853
Homology
BLAST of SMED30019020 vs. TrEMBL
Match: A0A5K3FC88 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 2.735e-47
Identity = 114/333 (34.23%), Postives = 164/333 (49.25%), Query Frame = 2
Query:  344 ISKIQLGGTPNRKVANLN---SADPRTA-----RLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLI-------------------------------------CHRKNDHFTIEESDRLSIEEGHTTDLMIKSSTETL----LDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLF 1195
            I ++ L G     ++ L    S +P  +     R+IL   +TTESER+  + QF + +CVVYEYQIG L+ L +++  A +VA LL+K I+ G I S S  +L L +L +    P S + K VKT  +      LSW L  ++T PGLR+N VGGENC NL IVT  L       E +RPDI+GYRNLI                                     C    D F  +E D ++++   + D      T+ +      HLH+ Y+ +FFV+ +R  +IDHL  C+SF  FC R+ I P++F N             LF +F++F
Sbjct:   34 IPRLNLTGNSTESISKLGINESTEPSCSGIERNRVILFLKSTTESERKRCLIQFAQHMCVVYEYQIGFLQGLKAVHSAADYVADLLLKSIQIGRITSFSPSELTLQLLHDVRCKPSSQM-KCVKTSPMTGTGEQLSWRLGDIWTRPGLRHNEVGGENCHNLNIVTHFLAPCLPDLETKRPDIYGYRNLIVLPTVESNILLSGESIYANASSNTVTVYTNCNSEAKECLSCFDIFPPDE-DLITLQHVGSVDSSPIRRTDVIKRHQRQHLHRAYRPVFFVIYERPSVIDHLTECESFAAFCKRQNICPVMFLNQETDLFEGRVCVNLFQHFDIF 364          
BLAST of SMED30019020 vs. TrEMBL
Match: W6VD49 (Uncharacterized protein OS=Echinococcus granulosus OX=6210 GN=EGR_00143 PE=4 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 3.549e-44
Identity = 115/366 (31.42%), Postives = 174/366 (47.54%), Query Frame = 2
Query:  212 RLPDSIKTDSETNSLVGSNNSINKVKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVANLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLICHRKNDHFT----IEESDRLSIEEGHTTDLMIKSSTETLLD---------HLHKCYKS-------------------------IFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLF 1195
            R+P S  +DS +N  +GS    +           P     S   + +++    P  +  ++  +     R++ +  +TTES+R+I + +F + LC+VYEYQIG L+ L +++  A +VA +LI+ I++G I S S  +L L +L +S   P S   K +KT  +      LSW L +++T PGLR+N VGGENC NL IVT  L   +T  E +RPDI+GYRNLI     +  T    + E+  L+     T D  I  +     D          + K  KS                           FV+ +R  +IDHL  C+SF  FC R+ I P++F N             LF NF++F
Sbjct:   13 RIPLSENSDSNSNLDIGSQTDFSH----------PHFPDFSNESLPRLEANDLPFPQFHDIEQS-----RILSILRSTTESDRKISIHRFAQHLCIVYEYQIGFLQGLKAVHSAADYVAEVLIRSIQSGRISSFSPNELTLQLLHDSGCRPSSQT-KCIKTSPMTGTGEQLSWRLGEIWTRPGLRHNEVGGENCHNLNIVTHFLAPCSTELEIKRPDIYGYRNLIVLPNTEPSTFFSDVYENANLNGATVFTNDSSIMGARSGYYDIFVPDEETIAVQKIDKSPSSPVRRGDVIKRQQGSTSIEPIDPFSFVIYERPSVIDHLTECESFAAFCKRQNICPVMFLNQETDLFEGRVCTSLFQNFDIF 362          
BLAST of SMED30019020 vs. TrEMBL
Match: A0A0R3X598 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS8608 PE=4 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 4.684e-40
Identity = 117/343 (34.11%), Postives = 156/343 (45.48%), Query Frame = 2
Query:  128 KVLLNLKWFVKHLCKVFEEKLQ------------RRICKYRLPD---------------------------SIKTDSETNSLVGSNNSI-----------------NKVKWNRSLTNSPLLKRLSLTKI-------------SKIQLGGTPNRKVANLNSAD--------------PRTA-------RLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLI 886
            KVLL+LKWF+  L K FE KLQ            R I    L                             S+ TD +  SL G                      N+    RSLTNSP + R+SLT+I             S   LG     +   L+  D              P +        R++L+  +TTESER I + +F + LCVVYEYQIG L+ L +++  A +VA +LI+ I+ G I SLS  +L L +L ++   P S   K +KT  +      LSW L +++T PGLR+N VGGENC NL IVT  L   +   E +RPDI+GYRNLI
Sbjct:   11 KVLLHLKWFINRLAKSFEHKLQTSGTLGLHPSSERHISNSPLASQSNLRSLNSPGRSPIAAKSLAGNLKNRSLSTDGDRLSLSGKTPDFECGVHRRSKMLPRLVNQNRSPKVRSLTNSPFMMRISLTRILSGKRIPLSECSESNSDLGEGSQSEFPQLHFPDFSDESLSGLDVKDLPFSPFHDIEQFRILLILRSTTESERRISISRFAQHLCVVYEYQIGFLQGLKAVHSAADYVADVLIRSIRTGRISSLSPNELTLQLLYDTSCKPSSQT-KCIKTSPMTGTGEQLSWRLGEIWTRPGLRHNEVGGENCHNLNIVTHFLAPCSAELEIKRPDIYGYRNLI 352          

HSP 2 Score: 68.1662 bits (165), Expect = 2.090e-8
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 2
Query:  974 SSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLF 1195
            S++  L  HLH+ Y+ +FFV+ +R  +IDHL  C+SF  FC R+ I P++F N             LF NF++F
Sbjct:  452 STSVNLRQHLHRAYRPVFFVIYERPSVIDHLTECESFAAFCKRQNICPVMFLNQETDLFEGRTCANLFQNFDIF 525          
BLAST of SMED30019020 vs. TrEMBL
Match: A0A183AUB1 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS10546 PE=4 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 1.615e-37
Identity = 81/176 (46.02%), Postives = 108/176 (61.36%), Query Frame = 2
Query:  443 TTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLIC---HRKNDHFTIEESDRLSIEEGHTTD 961
            T+E  R+  +  FG+ LC++YEYQ+GML  L+SI+  A +V  +++K IK G I  LSF++L  +IL +  L P +Y +K VKT  + V N  L+W L +++T PGLR+N VGGENC NL+IVT  L    T GE  R DI+GYRNLI    HR       EE D     EGH  D
Sbjct:   36 TSEVTRQKQLSAFGQQLCIIYEYQLGMLRGLNSIHAAADYVVDVILKAIKLGRITDLSFDQLTPAILFDVGLRPSTY-SKCVKTCPLAVTNEQLTWRLGEMWTLPGLRHNEVGGENCHNLSIVTHFLSTSMTGGEASRSDIYGYRNLILLMKHRS------EEPD----SEGHPID 200          

HSP 2 Score: 71.2478 bits (173), Expect = 1.739e-9
Identity = 30/86 (34.88%), Postives = 53/86 (61.63%), Query Frame = 2
Query:  968 IKSSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFP----NFNLFHISVTS 1213
            + S   T+  HLH+ Y+ +FFV+ +R  +I+HL  C SF  FC R+ ++P++F N +    ++  D++L+P    NF+LF ++V  
Sbjct:  290 VTSGASTIRQHLHRAYRPVFFVIFNRPSVIEHLTECDSFAAFCKRQSLSPVMFLNQT----TRIFDEKLYPCIYQNFDLFTVTVAG 371          
BLAST of SMED30019020 vs. TrEMBL
Match: A0A0R3SQP0 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS7403 PE=4 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 3.309e-37
Identity = 90/223 (40.36%), Postives = 124/223 (55.61%), Query Frame = 2
Query:  296 RSLTNSPLLKRLSLTKI---SKIQLG-----------GTP------------NRKVANLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLI 886
            RSLTNSP + RLSLT+I    +I L            G P            N  + +    D    R++L+   TTESER   V +F + +CVVYEYQIG L+ L S++  A ++A +L+K ++ G I SLS  ++ L++L   +  P S  NK +KT  +      LSW L +++T PGLR+N VGGENC NL IVT  L       E +RPD++GYRNLI
Sbjct:  121 RSLTNSPFMMRLSLTRILSNKRIPLSEYSDSNSDFDIGLPSDFPQLHFSDFSNGSLPSSPFQDVEKLRVLLILKNTTESERRFNVTKFAQHICVVYEYQIGFLQGLKSVHCAADYIAEVLVKAVRIGRITSLSTLEMTLTLLHEEDCRPSSQ-NKCIKTSPMTGTGEQLSWRLSEIWTKPGLRHNEVGGENCHNLNIVTHFLAPCAAELELKRPDVYGYRNLI 342          

HSP 2 Score: 65.855 bits (159), Expect = 1.108e-7
Identity = 28/74 (37.84%), Postives = 43/74 (58.11%), Query Frame = 2
Query:  974 SSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLF 1195
            S++  L  HLH+ Y+ +FFV+ +R  +IDHL  C+SF  FC R+ I P++F N             LF +F++F
Sbjct:  442 STSVNLRQHLHRAYRPVFFVIYERPSVIDHLTECESFAAFCKRQNICPVMFLNQETDLFEGRVCPSLFQHFDIF 515          
BLAST of SMED30019020 vs. Planmine SMEST
Match: SMESG000015266.1 (SMESG000015266.1)

HSP 1 Score: 823.157 bits (2125), Expect = 0.000e+0
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 2
Query:  101 MDAKYCECQKVLLNLKWFVKHLCKVFEEKLQRRICKYRLPDSIKTDSETNSLVGSNNSINKVKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVANLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLICHRKNDHFTIEESDRLSIEEGHTTDLMIKSSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLFHISVTSKPLENNVKSSLTVAXXXXXXXFCSECRILSKVYDNHAFMC 1333
            MDAKYCECQKVLLNLKWFVKHLCKVFEEKLQRRICKYRLPDSIKTDSETNSLVGSNNSINKVKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVANLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLICHRKNDHFTIEESDRLSIEEGHTTDLMIKSSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLFHISVTSKPLENNVKSSLTVADDDDSSSFCSECRILSKVYDNHAFMC
Sbjct:    1 MDAKYCECQKVLLNLKWFVKHLCKVFEEKLQRRICKYRLPDSIKTDSETNSLVGSNNSINKVKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVANLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLICHRKNDHFTIEESDRLSIEEGHTTDLMIKSSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLFHISVTSKPLENNVKSSLTVADDDDSSSFCSECRILSKVYDNHAFMC 411          
BLAST of SMED30019020 vs. Planmine SMEST
Match: SMESG000069112.1 (SMESG000069112.1)

HSP 1 Score: 317.39 bits (812), Expect = 1.248e-103
Identity = 185/398 (46.48%), Postives = 254/398 (63.82%), Query Frame = 2
Query:  101 MDAKYCECQKVLLNLKWFVKHLCKVFEEKLQRRICKYRLP--DSIKTDSETNS------LVGSNNSINK-VKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVANLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLI----------------CHRKNDHFTIEESDRLSIEEGHTTDLMIKSSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLFHISVTSKP 1219
            M+ KYCE QK+LLNLKWFVK  C++FEEKL+R   K++L    S+      N       L  S  S  K   W  SLTNSPL+KRLS  +    +  G+P +K  +  S+    +RL LL  +T++SERE ++K+FGE + VVYEYQIGML SL S+++V  +V  LL+K IK GV+  L   +LN +IL +S + P+S  +K VKT+ I + +N L+W +  +FTHPGLR+N VGGE+CSNL+IVT LL G    GE RRPDI+GYRNLI                C++     ++ +S  +S    ++T+    +ST +LLDH+HKCYKSIFFV+ DR  +I+HL+TCQSF+ FC +ELITPI+F N  +    K+  K LF NF+LF+I VT+ P
Sbjct:    1 MENKYCEYQKILLNLKWFVKQFCRLFEEKLERHCTKHQLKMTQSLLVGEPANGEPCISPLSSSTQSTPKHASWTHSLTNSPLVKRLSALQ----RHSGSPVKKSNSFRSSAINISRLTLLKESTSQSEREKHLKRFGEHVSVVYEYQIGMLSSLQSVSVVVIYVVDLLLKAIKKGVLMGLDSAQLNPTILFDSTVMPKS-GSKLVKTVGIRIHSNSLTWRVSNIFTHPGLRHNQVGGEDCSNLSIVTHLLTGFCPFGESRRPDIYGYRNLIVVPKRRISKKTEKGTNCNQTAYEISLYDSATISHARTNSTESTPTTSTNSLLDHIHKCYKSIFFVIFDRPTVIEHLMTCQSFRVFCTKELITPIMFLNAFNEITVKSCHKSLFHNFDLFNICVTANP 393          
BLAST of SMED30019020 vs. Planmine SMEST
Match: SMESG000037087.1 (SMESG000037087.1)

HSP 1 Score: 239.195 bits (609), Expect = 9.892e-74
Identity = 153/436 (35.09%), Postives = 236/436 (54.13%), Query Frame = 2
Query:  125 QKVLLNLKWFVKHLCKVFEEKLQRRICKYRL-PDSIKTDSETNSLVGSNN--------SINKVKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVA----NLNSADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINLVASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIAIGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGECRRPDIFGYRNLICHRKNDHFTIEESD---------RLSIE----EGHTTDLMIKSSTETLLDHLHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDLSKNADKRLFPNFNLFHISVT------SKPLENNVKSSLTVAXXXXXXXFCSECR-ILSKVYDNHAFMC 1333
             ++L N+KWFVK LC +FEE LQ ++ K +L P ++    + N L  +          +I+K  W RSLTNSP+++R +  + S+   G TP +K +    +L   D +  RL+L+   T+ESER   ++QF + + V+YEYQIGML+S+S +  V  FV  L+IK I+   I  L+  +LN +I   +   P+S  +K VKT+ +G    + SW +  +F+  GLR+N VGG++CSNLTI T       +     RPDI+GYRN+I   +N  +  E SD         +L ++          L+ +   E  +DH HKCYKS+FF++ DR  IIDHL  C SF+ FC RE I P++  ++   + +      L   F++  +S         KP E  +  + +  D+        E + +L+KVYDN AF+C
Sbjct:   14 HQILRNIKWFVKQLCSIFEETLQSKLYKSKLSPANLNIKIDVNCLPQNYKLSPAKQLTTIHKPNWCRSLTNSPIMRRFTPMRSSR---GSTPVKKFSIPPHHLKCEDVQLTRLLLIYTQTSESERIDQIQQFAQHVGVIYEYQIGMLKSVSLVMEVVRFVVDLIIKAIRMNFITELNSRQLNPTIFFLNIPLPKS-GSKVVKTLQLGPGTEF-SWRISDMFSRSGLRHNRVGGQDCSNLTISTHFYATSGSASYSYRPDIYGYRNIIA-IENSEYVCENSDETIKLYQLSKLDLDTLENPPDNGSLVGRDLEEQCMDHWHKCYKSMFFLICDRWEIIDHLENCDSFRVFCAREKICPVMQISLIPENFTYQQISALLQIFDVLSVSTNNNVIGDGKPDELCISRNHSRPDNKGDDFTDDETKGLLTKVYDNPAFVC 443          
The following BLAST results are available for this feature:
BLAST of SMED30019020 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K3FC882.735e-4734.23Uncharacterized protein OS=Mesocestoides corti OX=... [more]
W6VD493.549e-4431.42Uncharacterized protein OS=Echinococcus granulosus... [more]
A0A0R3X5984.684e-4034.11Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
A0A183AUB11.615e-3746.02Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A0R3SQP03.309e-3740.36Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
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BLAST of SMED30019020 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019020 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000015266.10.000e+0100.00SMESG000015266.1[more]
SMESG000069112.11.248e-10346.48SMESG000069112.1[more]
SMESG000037087.19.892e-7435.09SMESG000037087.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019020 ID=SMED30019020|Name=SMED30019020|organism=Schmidtea mediterranea sexual|type=transcript|length=1404bp
GTAATTCAAATTTTATTAAATGCTTATGTGATAAAATTTAATATTTAGAT
TAAAATGTTGGAAAACATTTAGTCAATTATTAAGGAAGTCAGACTAATAA
ATGGATGCCAAATATTGTGAGTGCCAGAAAGTGTTACTTAATTTGAAATG
GTTCGTGAAACACTTATGTAAAGTTTTTGAGGAAAAATTACAGCGCAGAA
TTTGCAAATATCGACTTCCAGATTCTATTAAAACAGACAGTGAAACGAAT
TCCTTGGTTGGCAGTAATAACAGTATTAATAAAGTCAAATGGAATCGTTC
TTTGACAAATTCACCGTTATTAAAGAGATTATCACTAACAAAAATTAGCA
AAATTCAACTTGGCGGAACTCCCAACAGAAAAGTGGCAAACCTGAATTCA
GCAGACCCAAGAACAGCTCGATTGATTTTGTTGAGTGCAACAACAACTGA
AAGTGAAAGAGAGATTTATGTTAAACAATTTGGAGAAAGTTTGTGCGTCG
TTTACGAATATCAAATAGGAATGTTAGAAAGTCTCTCAAGTATTAATTTG
GTCGCAAGTTTCGTAGCCAGTCTTTTGATAAAAGTAATCAAAAATGGAGT
CATTGACTCATTGTCCTTTGAAAAATTGAATTTGTCTATACTTCTAAATA
GTGAACTTTACCCACGAAGTTATGTAAATAAAAGCGTTAAAACAATAGCT
ATTGGGGTTGCCAATAACTATTTAAGTTGGAATCTCAGACAATTATTTAC
GCATCCCGGACTCAGGTACAATCATGTTGGAGGAGAAAACTGCTCAAATT
TGACAATAGTCACACAACTACTATGCGGTAAAACCACAACGGGTGAATGT
CGACGGCCAGATATTTTTGGCTATAGAAATTTAATCTGTCATCGGAAAAA
TGACCATTTCACTATTGAAGAAAGTGATAGACTGTCGATTGAAGAAGGTC
ATACAACTGATCTAATGATCAAAAGTTCAACGGAAACTTTGCTTGACCAT
TTGCACAAATGTTACAAGTCTATTTTCTTCGTTGTTCTTGACAGAACTGT
AATAATTGACCATTTGTTGACTTGTCAGTCATTTAAAACGTTTTGTTTAC
GAGAACTTATTACTCCGATTTTATTTGCCAATGTTTCTGATCCTGATCTA
TCAAAAAATGCTGACAAACGTCTCTTTCCGAATTTCAATTTGTTCCATAT
ATCTGTCACATCAAAACCGTTAGAGAACAATGTGAAATCCTCTTTGACGG
TAGCGGATGATGACGACTCGTCTTCCTTCTGTTCTGAATGTAGAATTTTA
TCGAAAGTTTATGATAATCATGCTTTTATGTGTTAATATTAGATTTAGTA
TTTGTTTTTTATAAAAAAGATAGAATATCCTACAATTTTTGCATTATAAT
AAAG
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protein sequence of SMED30019020-orf-1

>SMED30019020-orf-1 ID=SMED30019020-orf-1|Name=SMED30019020-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=412bp
MDAKYCECQKVLLNLKWFVKHLCKVFEEKLQRRICKYRLPDSIKTDSETN
SLVGSNNSINKVKWNRSLTNSPLLKRLSLTKISKIQLGGTPNRKVANLNS
ADPRTARLILLSATTTESEREIYVKQFGESLCVVYEYQIGMLESLSSINL
VASFVASLLIKVIKNGVIDSLSFEKLNLSILLNSELYPRSYVNKSVKTIA
IGVANNYLSWNLRQLFTHPGLRYNHVGGENCSNLTIVTQLLCGKTTTGEC
RRPDIFGYRNLICHRKNDHFTIEESDRLSIEEGHTTDLMIKSSTETLLDH
LHKCYKSIFFVVLDRTVIIDHLLTCQSFKTFCLRELITPILFANVSDPDL
SKNADKRLFPNFNLFHISVTSKPLENNVKSSLTVADDDDSSSFCSECRIL
SKVYDNHAFMC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head