Protein ENL

Overview
NameProtein ENL
Smed IDSMED30019012
Length (bp)2565
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein ENL (SMED30019012) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein ENL (SMED30019012) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein ENL (SMED30019012) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein ENL (SMED30019012) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019012

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30019012h1SMcG0010948 dd_Smed_v4_14072_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019012h1SMcG0010948 dd_Smed_v4_14072_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019012h1SMcG0010948 dd_Smed_v4_14072_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Protein ENL vs. Planmine SMEST
Match: SMESG000057165.1 (SMESG000057165.1)

HSP 1 Score: 1237.25 bits (3200), Expect = 0.000e+0
Identity = 720/723 (99.59%), Postives = 720/723 (99.59%), Query Frame = 1 
Query:  205 KRVVFILHNTFQEPYQIRKKPPXXXXXXXXXXXXMQINIKILNRKIPLPYFIQLMPNKVTTIQFHHSVWMVKPKELSKVLEIGGIPMEKFDNNDDEMKALTKILEYVKRHSDRPLDPSIFYPKVYERVFQKIKRLEASETSSSFMYNPDNDVAHSDDSSSTRQSHNVKSHNLLKHNKYESLLNEKSKMKSSKTFSEFMPSPKIDSHRSIDFEVKKELKRKKNHYESSSESDSNVCPENPFGSQKKIKKNDGYSRINKHEEVKLEKTIASRPVEKANSNFPKQESIIKTESVVKSVNKQPMSSKPKSTDKSNLKQLKDPHKLKLKIKDNSNPDSFNKIFEAQDILVVNRNKSNFLKNKDEVNVKRTKQDPSVKHFERKNSTPIKMTKESVTERSKKNTDLFGEENVGXXXXXXXXXXXXXNPMKEARLTNKPNPTAIDSNTKSVNSNSKMKLPEKTIPEQKKNRENETNKIQNPKSKKPNSNVYXXXXXXXXXXXXXXXXXXXKSSSDNEIDHDMFKPKKKLDLSNSDQTNVVVKQLFXXXXXXXXXXXXXXXXXXMFXXXXXXXXXXXXXPHQNKRQNXXXXXXXXXXXXXNEPPRGKDNNVSRSQSPDLKLETQKLNNSDILNKGNQDGHEEAALSYKERRRLAKQLIKINDKDFFEKLQRLLLTSYRNKERSLRFDPETMKLDFHMGEISDKLAFIIKAMVDEYFEEHLQSQAEDTSSHGF 2373
            KRV FILHNTFQEPYQIRKKPPFEIEEEGFGNFEMQINIKILNRKIPLPYFIQLMPNKVTTIQFHHSVWMVKPKELSKVLEIGGIPMEKFDNNDDEMKALTKILEYVKRHSDRPLDPSIFYPKVYERVFQKIKRLEASETSSSFMYNPDNDVAHSDDSSSTRQSHNVKSHNLLKHNKYESLLNEKSKMKSSKTFSEFMPSPKIDSHRSIDFEVKKELKRKKNHYESSSESDSNVCPENPFGSQKKIKKNDGYSRINKHEEVKLEKTIASRPVEKANSNFPKQESIIKTESVVKSVNKQPMSSKPKSTDKSNLKQLKDPHKLKLKIKDNSNPDSFNKIFEAQDILVVNRNKSNFLKNKDEVNVKRTKQDPSVKHFERKNSTPIKMTKESVTERSKK TDLFGEENVGKKSKSIKQEEKIKNPMKEARLTNKPNPTAIDSNTKSVNSNSKMKLPEKTIPEQKKNRENETNKIQNPKSKKPNSNVYSRNLSRSSTSGSDHGSAFSKSSSDNEIDHDMFKPKKKLDLSNSDQTNVVVKQLFGDDKSD SDEDDDDEIDDMFNSIKNSDKKSINKPHQNKRQNSSIDKSDDDISSDNEPPRGKDNNVSRSQSPDLKLETQKLNNSDILNKGNQDGHEEAALSYKERRRLAKQLIKINDKDFFEKLQRLLLTSYRNKERSLRFDPETMKLDFHMGEISDKLAFIIKAMVDEYFEEHLQSQAEDTSSHGF
Sbjct:   47 KRVEFILHNTFQEPYQIRKKPPFEIEEEGFGNFEMQINIKILNRKIPLPYFIQLMPNKVTTIQFHHSVWMVKPKELSKVLEIGGIPMEKFDNNDDEMKALTKILEYVKRHSDRPLDPSIFYPKVYERVFQKIKRLEASETSSSFMYNPDNDVAHSDDSSSTRQSHNVKSHNLLKHNKYESLLNEKSKMKSSKTFSEFMPSPKIDSHRSIDFEVKKELKRKKNHYESSSESDSNVCPENPFGSQKKIKKNDGYSRINKHEEVKLEKTIASRPVEKANSNFPKQESIIKTESVVKSVNKQPMSSKPKSTDKSNLKQLKDPHKLKLKIKDNSNPDSFNKIFEAQDILVVNRNKSNFLKNKDEVNVKRTKQDPSVKHFERKNSTPIKMTKESVTERSKKITDLFGEENVGKKSKSIKQEEKIKNPMKEARLTNKPNPTAIDSNTKSVNSNSKMKLPEKTIPEQKKNRENETNKIQNPKSKKPNSNVYSRNLSRSSTSGSDHGSAFSKSSSDNEIDHDMFKPKKKLDLSNSDQTNVVVKQLFGDDKSDCSDEDDDDEIDDMFNSIKNSDKKSINKPHQNKRQNSSIDKSDDDISSDNEPPRGKDNNVSRSQSPDLKLETQKLNNSDILNKGNQDGHEEAALSYKERRRLAKQLIKINDKDFFEKLQRLLLTSYRNKERSLRFDPETMKLDFHMGEISDKLAFIIKAMVDEYFEEHLQSQAEDTSSHGF 769

HSP 2 Score: 69.3218 bits (168), Expect = 8.869e-12
Identity = 33/37 (89.19%), Postives = 34/37 (91.89%), Query Frame = 3 
Query:   93 DI*FVTFLKSIYEVRPKKHPTSNKTHHWKLRIETSKS 203
            +I FV FLKSIYEVRPKKHPTSNKTHHWKLRIET KS
Sbjct:    6 EIPFVIFLKSIYEVRPKKHPTSNKTHHWKLRIETLKS 42
The following BLAST results are available for this feature:
BLAST of Protein ENL vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein ENL vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000057165.18.869e-1299.59SMESG000057165.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019012 ID=SMED30019012|Name=Protein ENL|organism=Schmidtea mediterranea sexual|type=transcript|length=2565bp
AGTAAATGTTGCAAAAAGAAATTCCTGTTAGTCATTAATTTATATTACAA
TAAAGAGATAAATTATCTCAGAAAAAAATATTATATATGTTTGATATTTA
GTTTGTCACCTTCCTTAAATCAATTTATGAAGTTAGACCTAAAAAGCATC
CAACAAGTAATAAAACTCATCATTGGAAGTTAAGAATTGAAACATCAAAG
TCCAAAAAGAGTTGTATTTATTTTGCATAATACATTTCAGGAGCCTTATC
AAATTCGGAAAAAGCCTCCTTTTGAGATTGAGGAAGAAGGATTTGGTAAC
TTTGAAATGCAAATTAATATTAAAATACTAAATCGAAAAATTCCCTTGCC
ATATTTTATTCAGCTGATGCCCAATAAAGTGACAACCATTCAATTTCATC
ATTCTGTATGGATGGTTAAACCTAAAGAATTAAGCAAAGTTCTGGAAATA
GGAGGCATTCCGATGGAAAAATTCGATAATAACGATGATGAAATGAAAGC
GCTTACGAAAATACTCGAGTATGTCAAACGACACAGTGATCGACCTCTAG
ATCCTTCAATATTTTATCCCAAGGTCTATGAACGTGTGTTCCAAAAGATC
AAGCGTCTTGAGGCATCTGAGACGTCATCATCATTCATGTACAATCCCGA
TAATGATGTAGCTCATAGTGATGATTCCAGTAGTACTCGTCAGTCCCATA
ATGTTAAATCGCACAATCTCCTAAAACACAATAAATACGAGTCTTTACTG
AATGAAAAATCTAAAATGAAGTCATCTAAAACCTTTAGTGAATTTATGCC
TTCTCCGAAAATCGATTCACATAGATCAATAGATTTTGAAGTGAAAAAAG
AATTGAAACGCAAAAAAAATCATTATGAATCTAGTTCAGAAAGTGATTCG
AATGTTTGTCCTGAGAATCCCTTTGGCTCCCAAAAGAAAATAAAGAAAAA
TGATGGATATAGCAGAATAAATAAGCATGAAGAAGTTAAGTTGGAGAAAA
CAATTGCCTCCAGGCCAGTAGAAAAAGCCAATTCTAATTTTCCCAAGCAA
GAAAGTATAATAAAAACAGAAAGCGTTGTTAAAAGTGTTAATAAACAACC
TATGTCATCTAAACCCAAATCTACAGACAAATCTAATTTAAAACAACTGA
AAGATCCACATAAATTGAAACTTAAAATTAAAGACAATTCTAACCCAGAT
TCATTCAACAAAATATTCGAAGCTCAAGATATTTTGGTTGTAAATAGAAA
CAAGTCAAATTTTTTAAAAAATAAAGACGAAGTAAATGTCAAACGAACTA
AACAAGACCCCAGTGTAAAACATTTCGAAAGAAAAAACTCGACACCAATT
AAAATGACAAAAGAAAGTGTAACAGAGCGGTCCAAAAAAAATACCGATCT
TTTTGGTGAAGAAAATGTTGGTAAGAAATCGAAATCTATCAAGCAAGAAG
AAAAAATTAAAAATCCAATGAAAGAAGCCAGGTTGACAAATAAACCAAAT
CCTACTGCAATTGATTCCAATACGAAATCCGTCAATTCCAACAGTAAAAT
GAAACTACCAGAAAAAACAATCCCTGAACAGAAGAAAAATAGAGAAAATG
AAACCAATAAAATTCAAAATCCGAAATCGAAAAAACCTAATTCTAATGTT
TATTCAAGAAATTTATCGAGATCGAGTACCAGTGGATCTGATCATGGAAG
TGCATTTTCAAAATCTTCTTCCGACAATGAGATTGATCATGATATGTTCA
AACCGAAGAAAAAACTCGATTTATCCAATTCTGATCAAACTAATGTCGTT
GTGAAGCAGTTGTTCGGAGATGATAAAAGTGACGGTAGTGATGAAGATGA
TGATGATGAAATCGATGACATGTTTAATTCGATAAAGAATTCCGACAAAA
AGTCTATTAATAAACCCCATCAAAACAAGCGACAAAATAGTTCTATTGAT
AAATCAGATGACGATATTTCATCGGATAATGAACCGCCGCGTGGCAAAGA
CAATAATGTATCTAGATCACAATCTCCCGATCTCAAATTGGAAACACAGA
AACTGAATAATTCAGATATATTAAATAAAGGTAATCAAGATGGACACGAA
GAAGCTGCGTTGAGTTATAAAGAAAGACGGCGTTTAGCAAAACAGCTCAT
CAAAATAAACGATAAGGATTTCTTTGAAAAACTGCAGCGTCTTTTACTCA
CTTCATATCGAAATAAAGAAAGAAGTTTGCGATTTGATCCTGAAACGATG
AAACTTGACTTTCACATGGGTGAAATTTCAGATAAATTGGCTTTTATAAT
TAAAGCTATGGTGGATGAATATTTTGAAGAGCACCTCCAATCTCAGGCAG
AAGATACTTCTAGTCATGGTTTCTAATGTCCTTTTGTAAATATAGATTAT
GGTTTGATGCTTAATATAAACTTTTCTTTGCTTTTGAATGGATATTCTGT
AAATAATACATTGATACAGTTTATGTTGGAATGTTTTCATTTGTACAGGA
TTATTTCATTTACCAACAACATTTGTACAATCAAGATGTTTTACTGGAAT
TGAAATTTGTATAAG
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protein sequence of SMED30019012-orf-1

>SMED30019012-orf-1 ID=SMED30019012-orf-1|Name=SMED30019012-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=690bp
MQINIKILNRKIPLPYFIQLMPNKVTTIQFHHSVWMVKPKELSKVLEIGG
IPMEKFDNNDDEMKALTKILEYVKRHSDRPLDPSIFYPKVYERVFQKIKR
LEASETSSSFMYNPDNDVAHSDDSSSTRQSHNVKSHNLLKHNKYESLLNE
KSKMKSSKTFSEFMPSPKIDSHRSIDFEVKKELKRKKNHYESSSESDSNV
CPENPFGSQKKIKKNDGYSRINKHEEVKLEKTIASRPVEKANSNFPKQES
IIKTESVVKSVNKQPMSSKPKSTDKSNLKQLKDPHKLKLKIKDNSNPDSF
NKIFEAQDILVVNRNKSNFLKNKDEVNVKRTKQDPSVKHFERKNSTPIKM
TKESVTERSKKNTDLFGEENVGKKSKSIKQEEKIKNPMKEARLTNKPNPT
AIDSNTKSVNSNSKMKLPEKTIPEQKKNRENETNKIQNPKSKKPNSNVYS
RNLSRSSTSGSDHGSAFSKSSSDNEIDHDMFKPKKKLDLSNSDQTNVVVK
QLFGDDKSDGSDEDDDDEIDDMFNSIKNSDKKSINKPHQNKRQNSSIDKS
DDDISSDNEPPRGKDNNVSRSQSPDLKLETQKLNNSDILNKGNQDGHEEA
ALSYKERRRLAKQLIKINDKDFFEKLQRLLLTSYRNKERSLRFDPETMKL
DFHMGEISDKLAFIIKAMVDEYFEEHLQSQAEDTSSHGF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR005033YEATS
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 397..418
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 323..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 275..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 419..437
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 526..596
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 526..564
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..274
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 565..591
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 323..494
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 472..489
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 438..471