Neuralized, putative

Overview
NameNeuralized, putative
Smed IDSMED30019009
Length (bp)1702
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Neuralized, putative (SMED30019009) t-SNE clustered cells

Violin plots show distribution of expression levels for Neuralized, putative (SMED30019009) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Neuralized, putative (SMED30019009) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Neuralized, putative (SMED30019009) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30019009

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30019009h1SMcG0018151 Contig5498GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
muscle cellSMED30019009h1SMcG0018151 dd_Smed_v4_1934_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Neuralized, putative vs. Ensembl Human
Match: NEURL1B (neuralized E3 ubiquitin protein ligase 1B [Source:HGNC Symbol;Acc:HGNC:35422])

HSP 1 Score: 97.8265 bits (242), Expect = 7.334e-21
Identity = 69/231 (29.87%), Postives = 118/231 (51.08%), Query Frame = 1 
Query:   82 IMGNTPNSKSQPVRPPM-----KIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTP---TEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNI 735
            ++   P     P R P+     + H Q  GKN+ +        R   + +G+ F  RP+   E++ + ++ +   W+GALRFGFT H+P  +S  A+ IP+Y CPDL+ R G + + + + +L ++D ++ YW +  G V   VN+   V+  C +  +   +W ++DVYG T EV    L+       TP   ++ RF + L  S    +AN  + EL ++  V K G++
Sbjct:   18 LLATRPCCGPGPERRPVLGEAPRFHAQAKGKNVRLDGHSRRATRRNSFCNGVTFTQRPIRLYEQVRLRLVAVRPGWSGALRFGFTAHDPSLMS--AQDIPKYACPDLVTRPGYWAKALPE-NLALRDTVLAYWADRHGRVFYSVNDGEPVLFHCGV-AVGGPLWALIDVYGITDEVQL--LESAFADTLTPARLSQARFSACLPPSSHD-AANFDNNELENNQVVAKLGHL 241
[Close]
BLAST of Neuralized, putative vs. Ensembl Human
Match: NEURL1B (neuralized E3 ubiquitin protein ligase 1B [Source:HGNC Symbol;Acc:HGNC:35422])

HSP 1 Score: 94.7449 bits (234), Expect = 1.136e-20
Identity = 57/192 (29.69%), Postives = 98/192 (51.04%), Query Frame = 1 
Query:   82 IMGNTPNSKSQPVRPPM-----KIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPT 627
            ++   P     P R P+     + H Q  GKN+ +        R   + +G+ F  RP+   E++ + ++ +   W+GALRFGFT H+P  +S  A+ IP+Y CPDL+ R G + + + + +L ++D ++ YW +  G V   VN+   V+  C +  +   +W ++DVYG T EV       +     TP+
Sbjct:   18 LLATRPCCGPGPERRPVLGEAPRFHAQAKGKNVRLDGHSRRATRRNSFCNGVTFTQRPIRLYEQVRLRLVAVRPGWSGALRFGFTAHDPSLMS--AQDIPKYACPDLVTRPGYWAKALPE-NLALRDTVLAYWADRHGRVFYSVNDGEPVLFHCGV-AVGGPLWALIDVYGITDEVQLLGTLQSSPATTTPS 205
[Close]
BLAST of Neuralized, putative vs. Ensembl Human
Match: NEURL1 (neuralized E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:7761])

HSP 1 Score: 96.6709 bits (239), Expect = 2.162e-20
Identity = 67/256 (26.17%), Postives = 117/256 (45.70%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSAN--AVSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEK 879
            P+  H    G  + +     +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  H + +P+Y CPDL+ + G + + + +      + I +W++  G V  ++N+        G+     +W +VDVYG T  V    L D+  ++     PR +++LR       A+   +SV L D +     G+  A A    P P N++       +   +  +L    L+    V  L E+
Sbjct:   62 PLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--HPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFHRINDSAVMLFFSGVRTADPLWALVDVYGLTRGV---QLLDSELVLPDCLRPRSFTALRRPSLRREADDARLSVSLCDLNVPGADGDEAAPAAG-CPIPQNSLNSQHSRALPAQLDGDLRFHALRAGAHVRILDEQ 311
[Close]
BLAST of Neuralized, putative vs. Ensembl Human
Match: NEURL1 (neuralized E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:7761])

HSP 1 Score: 85.1149 bits (209), Expect = 2.342e-18
Identity = 45/157 (28.66%), Postives = 78/157 (49.68%), Query Frame = 1 
Query:  115 PVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEV 579
            P  P +      G  + +     +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  H + +P+Y CPDL+ + G + + + +      + I +W++  G V  ++N+        G+     +W +VDVYG T  V
Sbjct:   42 PATPLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--HPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFHRINDSAVMLFFSGVRTADPLWALVDVYGLTRGV 196
[Close]
BLAST of Neuralized, putative vs. Ensembl Human
Match: NEURL3 (neuralized E3 ubiquitin protein ligase 3 [Source:HGNC Symbol;Acc:HGNC:25162])

HSP 1 Score: 82.0333 bits (201), Expect = 8.688e-17
Identity = 50/178 (28.09%), Postives = 84/178 (47.19%), Query Frame = 1 
Query:  100 NSKSQPVRPPMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIV--KDVWPVVDVYGNTVEVTFRSLDDNVDLIYTP 624
            N+K+   R  ++ H +  G  + +        R   + DGI F  RPV  GE++ + VL+  S W G LR GFT  +P  +S     +P ++CPDL ++  ++   + +      D + +W++  G +  KVN   +     G+     +W V+DVYG T  +    LD     + TP
Sbjct:   10 NAKAP--REALRFHAEAKGAQVRLDTRGCIAHRRTTFHDGIVFSQRPVRLGERVALRVLREESGWCGGLRVGFTRLDPACVS--VPSLPPFLCPDLEEQSPTWAAVLPEGCALTGDLVRFWVDRRGCLFAKVNAGCRLLLREGVPVGAPLWAVMDVYGTTKAIEL--LDPTASRLPTP 181
[Close]
BLAST of Neuralized, putative vs. Ensembl Celegans
Match: F10D7.5 (pep chromosome:WBcel235:X:17383125:17391615:-1 gene:WBGene00017342.1 transcript:F10D7.5a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10D7.5)

HSP 1 Score: 81.2629 bits (199), Expect = 5.724e-16
Identity = 42/156 (26.92%), Postives = 82/156 (52.56%), Query Frame = 1 
Query:  118 VRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEV 579
             R P++ H IHG N+ +  +    KR E +  G+ F +RP+   E + + + ++ + W+G LRFG T  +P    +    +P + CPDL  + G + + + +      + + +++ + GE+   +N   +   L GI   + +W ++D+YGN+V V
Sbjct:   35 TRGPLQFHCIHGSNVVILKNGRLAKRRESFCKGLAFSNRPIEIDENVCLRLCEVGTNWSGVLRFGVTNDDPEMYRDIP--VPTFACPDLTTKDGYWAKALPERYSNEGNILHFYVNAHGELFYGINGSQKGMFLTGINVHRPMWLILDIYGNSVGV 188

HSP 2 Score: 57.7658 bits (138), Expect = 1.551e-8
Identity = 40/155 (25.81%), Postives = 78/155 (50.32%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADR-LSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV--YWLESTGEVSLKVNE-VMQKCQLHGIVKDVWPVVDVYGNTVEV 579
            P++ H + G ++T+   R ++ +    YS G  F  RP+ + EK+ I + ++   + G L FG T  +P +I     G+P     DL++    +V  I  I+L+ K   +  +W+  +GEV  +++    + C       +++   DVYG+T+ +
Sbjct:  249 PLRFHYVKGCHITLNPSRNIATRDQAEYSQGYVFTERPIKNNEKVMIMISQVQRLYEGGLAFGVTCCDPASI--RVAGLPDDSS-DLVEMPEYWV-GIKDIALQPKANSILSFWITDSGEVKFEIDSNGARTCLYVDNSLELYMYFDVYGSTLSI 399

HSP 3 Score: 51.9878 bits (123), Expect = 1.054e-6
Identity = 28/100 (28.00%), Postives = 41/100 (41.00%), Query Frame = 1 
Query: 1174 AASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            + S LP+      TT  +               RN     +E +   EC IC+D   ++V   CGH+C C  C   L+   G+  CP CR  ++   K Y
Sbjct:  518 STSRLPNRSSPATTTAMMSPPSFNPPPLPASFQRNEGNGAQEVNEGDECTICMDAPVNSVLYTCGHMCMCFECGRRLLTTKGT--CPICRAPVQDVIKTY 615
[Close]
BLAST of Neuralized, putative vs. Ensembl Celegans
Match: F10D7.5 (pep chromosome:WBcel235:X:17388272:17391561:-1 gene:WBGene00017342.1 transcript:F10D7.5e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10D7.5)

HSP 1 Score: 67.3958 bits (163), Expect = 5.512e-13
Identity = 33/132 (25.00%), Postives = 67/132 (50.76%), Query Frame = 1 
Query:  100 NSKSQPVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVN 495
            N  +   R P++ H IHG N+ +  +    KR E +  G+ F +RP+   E + + + ++ + W+G LRFG T  +P    +    +P + CPDL  + G + + + +      + + +++ + GE+   +N
Sbjct:   29 NQVTPGTRGPLQFHCIHGSNVVILKNGRLAKRRESFCKGLAFSNRPIEIDENVCLRLCEVGTNWSGVLRFGVTNDDPEMYRDIP--VPTFACPDLTTKDGYWAKALPERYSNEGNILHFYVNAHGELFYGIN 158
[Close]
BLAST of Neuralized, putative vs. Ensembl Celegans
Match: F10D7.5 (pep chromosome:WBcel235:X:17385978:17391561:-1 gene:WBGene00017342.1 transcript:F10D7.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10D7.5)

HSP 1 Score: 66.6254 bits (161), Expect = 2.048e-12
Identity = 32/126 (25.40%), Postives = 65/126 (51.59%), Query Frame = 1 
Query:  118 VRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVN 495
             R P++ H IHG N+ +  +    KR E +  G+ F +RP+   E + + + ++ + W+G LRFG T  +P    +    +P + CPDL  + G + + + +      + + +++ + GE+   +N
Sbjct:   35 TRGPLQFHCIHGSNVVILKNGRLAKRRESFCKGLAFSNRPIEIDENVCLRLCEVGTNWSGVLRFGVTNDDPEMYRDIP--VPTFACPDLTTKDGYWAKALPERYSNEGNILHFYVNAHGELFYGIN 158
[Close]
BLAST of Neuralized, putative vs. Ensembl Celegans
Match: F10D7.5 (pep chromosome:WBcel235:X:17383125:17386535:-1 gene:WBGene00017342.1 transcript:F10D7.5c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10D7.5)

HSP 1 Score: 57.7658 bits (138), Expect = 1.250e-8
Identity = 40/155 (25.81%), Postives = 78/155 (50.32%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADR-LSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV--YWLESTGEVSLKVNE-VMQKCQLHGIVKDVWPVVDVYGNTVEV 579
            P++ H + G ++T+   R ++ +    YS G  F  RP+ + EK+ I + ++   + G L FG T  +P +I     G+P     DL++    +V  I  I+L+ K   +  +W+  +GEV  +++    + C       +++   DVYG+T+ +
Sbjct:   69 PLRFHYVKGCHITLNPSRNIATRDQAEYSQGYVFTERPIKNNEKVMIMISQVQRLYEGGLAFGVTCCDPASI--RVAGLPDDSS-DLVEMPEYWV-GIKDIALQPKANSILSFWITDSGEVKFEIDSNGARTCLYVDNSLELYMYFDVYGSTLSI 219

HSP 2 Score: 50.447 bits (119), Expect = 2.371e-6
Identity = 23/67 (34.33%), Postives = 33/67 (49.25%), Query Frame = 1 
Query: 1273 RNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            RN     +E +   EC IC+D   ++V   CGH+C C  C   L+   G+  CP CR  ++   K Y
Sbjct:  371 RNEGNGAQEVNEGDECTICMDAPVNSVLYTCGHMCMCFECGRRLLTTKGT--CPICRAPVQDVIKTY 435
[Close]
BLAST of Neuralized, putative vs. Ensembl Celegans
Match: K11D12.9 (pep chromosome:WBcel235:V:5042408:5046203:-1 gene:WBGene00019649.1 transcript:K11D12.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K11D12.9)

HSP 1 Score: 52.373 bits (124), Expect = 4.926e-7
Identity = 30/72 (41.67%), Postives = 36/72 (50.00%), Query Frame = 1 
Query: 1288 KPREYDFR-------AECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSIL--CPTCRKKIEHSYKVYM 1476
            + R  DF+       AEC ICL T P  VF+PC H+  C  C D       S    CPTCR  IE+S  V+M
Sbjct:  335 RSRNRDFQRLQEKLVAECCICLATKPSIVFMPCRHLITCSGCYD------ASDFRECPTCRSTIENSITVFM 400
[Close]
BLAST of Neuralized, putative vs. Ensembl Fly
Match: neur (gene:FBgn0002932 transcript:FBtr0082002)

HSP 1 Score: 102.064 bits (253), Expect = 2.242e-22
Identity = 49/157 (31.21%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEG-IPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P+ +    EG +P+Y CPDL  R G + + + +      + + Y++   G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:   23 PPLQFHSVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPVTL----EGTLPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNGAGDVIYGINNEEKGVILTGIDTRSLLWTVIDIYGNCTGIEF 175

HSP 2 Score: 60.4622 bits (145), Expect = 2.864e-9
Identity = 51/178 (28.65%), Postives = 83/178 (46.63%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPR 636
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+
Sbjct:  286 PVPFHNTKGRNVRLSQDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPAMLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQ 454
[Close]
BLAST of Neuralized, putative vs. Ensembl Fly
Match: neur (gene:FBgn0002932 transcript:FBtr0082001)

HSP 1 Score: 102.064 bits (253), Expect = 2.267e-22
Identity = 49/157 (31.21%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEG-IPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P+ +    EG +P+Y CPDL  R G + + + +      + + Y++   G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:   23 PPLQFHSVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPVTL----EGTLPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNGAGDVIYGINNEEKGVILTGIDTRSLLWTVIDIYGNCTGIEF 175

HSP 2 Score: 60.4622 bits (145), Expect = 2.967e-9
Identity = 51/178 (28.65%), Postives = 83/178 (46.63%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPR 636
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+
Sbjct:  286 PVPFHNTKGRNVRLSQDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPAMLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQ 454
[Close]
BLAST of Neuralized, putative vs. Ensembl Fly
Match: neur (gene:FBgn0002932 transcript:FBtr0300414)

HSP 1 Score: 102.064 bits (253), Expect = 2.267e-22
Identity = 49/157 (31.21%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEG-IPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P+ +    EG +P+Y CPDL  R G + + + +      + + Y++   G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:   23 PPLQFHSVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPVTL----EGTLPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNGAGDVIYGINNEEKGVILTGIDTRSLLWTVIDIYGNCTGIEF 175

HSP 2 Score: 60.4622 bits (145), Expect = 2.967e-9
Identity = 51/178 (28.65%), Postives = 83/178 (46.63%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPR 636
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+
Sbjct:  286 PVPFHNTKGRNVRLSQDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPAMLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQ 454
[Close]
BLAST of Neuralized, putative vs. Ensembl Fly
Match: neur (gene:FBgn0002932 transcript:FBtr0082003)

HSP 1 Score: 101.679 bits (252), Expect = 3.692e-22
Identity = 49/157 (31.21%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEG-IPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P+ +    EG +P+Y CPDL  R G + + + +      + + Y++   G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:  105 PPLQFHSVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPVTL----EGTLPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNGAGDVIYGINNEEKGVILTGIDTRSLLWTVIDIYGNCTGIEF 257

HSP 2 Score: 60.4622 bits (145), Expect = 2.979e-9
Identity = 51/178 (28.65%), Postives = 83/178 (46.63%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPR 636
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+
Sbjct:  368 PVPFHNTKGRNVRLSQDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPAMLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQ 536
[Close]
BLAST of Neuralized, putative vs. Ensembl Fly
Match: neur (gene:FBgn0002932 transcript:FBtr0082004)

HSP 1 Score: 101.293 bits (251), Expect = 3.854e-22
Identity = 49/157 (31.21%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEG-IPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P+ +    EG +P+Y CPDL  R G + + + +      + + Y++   G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:  105 PPLQFHSVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPVTL----EGTLPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNGAGDVIYGINNEEKGVILTGIDTRSLLWTVIDIYGNCTGIEF 257

HSP 2 Score: 60.4622 bits (145), Expect = 3.029e-9
Identity = 51/178 (28.65%), Postives = 83/178 (46.63%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPR 636
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+
Sbjct:  368 PVPFHNTKGRNVRLSQDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPAMLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQ 536
[Close]
BLAST of Neuralized, putative vs. Ensembl Zebrafish
Match: neurl1ab (neuralized E3 ubiquitin protein ligase 1Ab [Source:ZFIN;Acc:ZDB-GENE-060929-1210])

HSP 1 Score: 96.2857 bits (238), Expect = 1.139e-20
Identity = 113/506 (22.33%), Postives = 186/506 (36.76%), Query Frame = 1 
Query:  115 PVRPPMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIV--KDVWPVVDVYGNTV-----------------EVTFRSLDD--------------------------NVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAP-QVNNL-----PEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRA-ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            P   P+  H    G  + +   + SV+R   + + I F SRPV   E++ + + K    W+GALR GF+  +P  IS  +  +PR+ CPDL+   G +   + +        + +WL++ G V  +VN         G+   + VW ++D+YG T                   VT   +DD                          N+ L +  ++   + S+  S   L    ++V   +    C      A  +   P        L   +V       LS       P  VN+      P++L D  R   W F ++   +V GD +       G V V+ NG R      V  ++  W+   +   ++ ++ +G  +   Y      +  +I       +A+ + S   +R T            S        +   P        EC+IC D   D+V   CGH+C C  C   L  E  +  CP CR  I    K+Y
Sbjct:    6 PAPLPLCFHANTKGSQIVMDKTQRSVRRIASFCNAITFTSRPVGVYEQVRLKITKTQGCWSGALRVGFSIVDPSNIS--SAWLPRFACPDLVSEQGFWGRALPEEHCEEGTILSFWLDNIGRVFYRVNGGSPIFFFSGVPAGEPVWGIIDIYGLTRGVHLLDCKMLPAEYLSPAVTHEGMDDWHLSSGCSEFDLQEDLPSFSSSPNALNLLLSHQLSDDLHFHSVHGSALRLLTEHIAVRYYNRREAC------ALVFTHRPLRCGECVFL---KVSLRSSGFLSYGFTSCNPAHVNSKHLPVDPDELLD--RKEFWAFSSMTSALVGGDIIGFRATAEGEVLVSHNGGRARREMCVDNSAPLWMIFHLQTHIMQISILGTSR--GYSPSCSETQRSIFSDASHLSANQIHSTAIRRCTDSSQPRPHSLSSSLGTAFGSLSSESPSCCALTGEECLICCDRPVDSVLYACGHMCVCSDCGVKL-TETSNPSCPVCRSPIRDIIKIY 495
[Close]
BLAST of Neuralized, putative vs. Ensembl Zebrafish
Match: neurl1ab (neuralized E3 ubiquitin protein ligase 1Ab [Source:ZFIN;Acc:ZDB-GENE-060929-1210])

HSP 1 Score: 94.7449 bits (234), Expect = 4.082e-20
Identity = 113/506 (22.33%), Postives = 185/506 (36.56%), Query Frame = 1 
Query:  115 PVRPPMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIV--KDVWPVVDVYGNTV-----------------EVTFRSLDD--------------------------NVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAP-QVNNL-----PEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRA-ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            P   P+  H    G  + +   + SV+R   + + I F SRPV   E++ + + K    W+GALR GF+  +P  IS  +  +PR+ CPDL+   G +   + +        + +WL++ G V  +VN         G+   + VW ++D+YG T                   VT   +DD                          N+ L +  ++   + S+  S   L    ++V   +    C      A  +   P        L   +V       LS       P  VN+      P++L D  R   W F ++   +V GD +       G V V+ NG R      V  ++  W+   +   +  ++ +G  +   Y      +  +I       +A+ + S   +R T            S        +   P        EC+IC D   D+V   CGH+C C  C   L  E  +  CP CR  I    K+Y
Sbjct:    6 PAPLPLCFHANTKGSQIVMDKTQRSVRRIASFCNAITFTSRPVGVYEQVRLKITKTQGCWSGALRVGFSIVDPSNIS--SAWLPRFACPDLVSEQGFWGRALPEEHCEEGTILSFWLDNIGRVFYRVNGGSPIFFFSGVPAGEPVWGIIDIYGLTRGVHLLDCKMLPAEYLSPAVTHEGMDDWHLSSGCSEFDLQEDLPSFSSSPNALNLLLSHQLSDDLHFHSVHGSALRLLTEHIAVRYYNRREAC------ALVFTHRPLRCGECVFL---KVSLRSSGFLSYGFTSCNPAHVNSKHLPVDPDELLD--RKEFWAFSSMTSALVGGDIIGFRATAEGEVLVSHNGGRARREMCVDNSAPLWMIFHLQTHIKQISILGTSR--GYSPSCSETQRSIFSDASHLSANQIHSTAVRRCTDSSQPRPHSLSSSLGTAFGSLSSESPSCCALTGEECLICCDRPVDSVLYACGHMCVCSDCGVKL-TETSNPSCPVCRSPIRDIIKIY 495
[Close]
BLAST of Neuralized, putative vs. Ensembl Zebrafish
Match: neurl1ab (neuralized E3 ubiquitin protein ligase 1Ab [Source:ZFIN;Acc:ZDB-GENE-060929-1210])

HSP 1 Score: 94.7449 bits (234), Expect = 4.270e-20
Identity = 113/506 (22.33%), Postives = 185/506 (36.56%), Query Frame = 1 
Query:  115 PVRPPMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIV--KDVWPVVDVYGNTV-----------------EVTFRSLDD--------------------------NVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAP-QVNNL-----PEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRA-ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            P   P+  H    G  + +   + SV+R   + + I F SRPV   E++ + + K    W+GALR GF+  +P  IS  +  +PR+ CPDL+   G +   + +        + +WL++ G V  +VN         G+   + VW ++D+YG T                   VT   +DD                          N+ L +  ++   + S+  S   L    ++V   +    C      A  +   P        L   +V       LS       P  VN+      P++L D  R   W F ++   +V GD +       G V V+ NG R      V  ++  W+   +   +  ++ +G  +   Y      +  +I       +A+ + S   +R T            S        +   P        EC+IC D   D+V   CGH+C C  C   L  E  +  CP CR  I    K+Y
Sbjct:   29 PAPLPLCFHANTKGSQIVMDKTQRSVRRIASFCNAITFTSRPVGVYEQVRLKITKTQGCWSGALRVGFSIVDPSNIS--SAWLPRFACPDLVSEQGFWGRALPEEHCEEGTILSFWLDNIGRVFYRVNGGSPIFFFSGVPAGEPVWGIIDIYGLTRGVHLLDCKMLPAEYLSPAVTHEGMDDWHLSSGCSEFDLQEDLPSFSSSPNALNLLLSHQLSDDLHFHSVHGSALRLLTEHIAVRYYNRREAC------ALVFTHRPLRCGECVFL---KVSLRSSGFLSYGFTSCNPAHVNSKHLPVDPDELLD--RKEFWAFSSMTSALVGGDIIGFRATAEGEVLVSHNGGRARREMCVDNSAPLWMIFHLQTHIKQISILGTSR--GYSPSCSETQRSIFSDASHLSANQIHSTAVRRCTDSSQPRPHSLSSSLGTAFGSLSSESPSCCALTGEECLICCDRPVDSVLYACGHMCVCSDCGVKL-TETSNPSCPVCRSPIRDIIKIY 518
[Close]
BLAST of Neuralized, putative vs. Ensembl Zebrafish
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:ZFIN;Acc:ZDB-GENE-050522-102])

HSP 1 Score: 92.4337 bits (228), Expect = 2.725e-19
Identity = 55/175 (31.43%), Postives = 92/175 (52.57%), Query Frame = 1 
Query:   91 NTPNSKSQPVRPPMKIH------QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTF 585
            +T  ++ +   PP+ I          GKN+ + A      R   + +GI F  RPV   EK+ + +  + + W+GALRFGFTT +P  +S     IP+Y CPDL+ R G + + + +  L ++D ++ +W +  G V   +N+   ++  C L  +   VW ++D+YG T EVT 
Sbjct:   28 STSGAERRTSAPPVSIESPRFHPHAKGKNIRLDAHLRRATRKNSFCNGITFSQRPVRLYEKVRLRLSGVHTGWSGALRFGFTTLDPGELS--LTDIPKYACPDLVTRPGYWAKALPE-RLAMRDNVLAFWADRHGRVFYSINDGEPILFHCGL-SVGCPVWAIIDIYGITQEVTL 198
[Close]
BLAST of Neuralized, putative vs. Ensembl Zebrafish
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:ZFIN;Acc:ZDB-GENE-050522-102])

HSP 1 Score: 92.4337 bits (228), Expect = 2.806e-19
Identity = 55/175 (31.43%), Postives = 92/175 (52.57%), Query Frame = 1 
Query:   91 NTPNSKSQPVRPPMKIH------QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTF 585
            +T  ++ +   PP+ I          GKN+ + A      R   + +GI F  RPV   EK+ + +  + + W+GALRFGFTT +P  +S     IP+Y CPDL+ R G + + + +  L ++D ++ +W +  G V   +N+   ++  C L  +   VW ++D+YG T EVT 
Sbjct:   47 STSGAERRTSAPPVSIESPRFHPHAKGKNIRLDAHLRRATRKNSFCNGITFSQRPVRLYEKVRLRLSGVHTGWSGALRFGFTTLDPGELS--LTDIPKYACPDLVTRPGYWAKALPE-RLAMRDNVLAFWADRHGRVFYSINDGEPILFHCGL-SVGCPVWAIIDIYGITQEVTL 217
[Close]
BLAST of Neuralized, putative vs. Ensembl Xenopus
Match: neurl1 (neuralized E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100036686])

HSP 1 Score: 103.99 bits (258), Expect = 7.133e-23
Identity = 110/516 (21.32%), Postives = 200/516 (38.76%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHG--IVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSL------------RLS-----------------------QFTLSAN-------------------AVSVELVDSDTVCKKGNI---KAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQV---NNL---PEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEI-QYIPVREESNRQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            P+  H    G  + +   + +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  + + +P+Y CPDL+ + G + + + +      + I +W++  G V  +VN+        G  I + +W ++DVYG T  V    LD  + +    + PR ++S+            RLS                       Q +L++                       ++ +D  TV +  +    +   +   P   N    + +N+ + S    LS  +    P     ++L   PE L D  R   W    V + +  GD +   I   G + ++ NG        V  +   W+   ++ +++ +  +G    T       PS P           SA+         +  +   I +   ++   +P +  +      +  EC IC + + D V   CGH+C C  C   L  +  +  CP CR+ I+   K Y
Sbjct:   45 PLLFHPHAKGSQIIMDTSQKAVKRQASFCNAITFSNRPVVIHEQVRLKITKKQCCWSGALRLGFTSKDPSRI--NPDTLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRVNDFGSMLFFSGVRITEPLWALIDVYGLTRGVEL--LDSEI-IPPDCSRPRSFTSIPRPSLRRETEDTRLSVSLCDLNLQEDNYQTMTTMCPIPQNSLNSQHSHLLPSQLESDLRFHQLRGAHIKTLDDQTVARLEHAREERTLVFTNRPLRINETIFVKINKSNSSRSGTLSYGVTSCDPSTLRPSDLPYNPESLVD--RKEFWAVCRVLVPLQSGDILGFVINGEGELHLSHNGTSTGMQVCVDCSQPLWMFFGLHGTIMQIRILGSTISTDNREASAPSSPTSAPNSPTAPCSAISDPILNSCVSAPMGSSINITAPNSPVSMPESPLSPSLSGSWSDECTICYENLVDTVIYSCGHMCLCYTCGLKL-KKMNNACCPICRRLIKDIIKTY 552
[Close]
BLAST of Neuralized, putative vs. Ensembl Xenopus
Match: kctd10 (potassium channel tetramerization domain containing 10 [Source:Xenbase;Acc:XB-GENE-970871])

HSP 1 Score: 95.1301 bits (235), Expect = 4.491e-20
Identity = 57/177 (32.20%), Postives = 92/177 (51.98%), Query Frame = 1 
Query:  142 QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPT-EPRFYSSLRLS 660
            Q  GKN+ +        R   + +GI F SRP+   EK+ + ++ +   W+GALRFGFT H+P  +    E IP+Y CPDL+ R G + + + +   +  + + +W++  G V   VN+   V+  C +  +   +W ++DVYG T EV        +D ++  T  P   SS RLS
Sbjct:   49 QAKGKNIRLDTYGRKAIRRNSFCNGITFTSRPIHLYEKVRLKLVSVHHGWSGALRFGFTIHDPAQMK--LEDIPKYACPDLVTRPGYWAKALPERFAQRDNILAFWVDRHGRVFYSVNDEEPVLFHCGV-KVSSPLWALIDVYGITQEVQL------LDSMFADTMAPSRLSSPRLS 216
[Close]
BLAST of Neuralized, putative vs. Ensembl Xenopus
Match: kctd10 (potassium channel tetramerization domain containing 10 [Source:Xenbase;Acc:XB-GENE-970871])

HSP 1 Score: 95.1301 bits (235), Expect = 5.090e-20
Identity = 57/177 (32.20%), Postives = 92/177 (51.98%), Query Frame = 1 
Query:  142 QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPT-EPRFYSSLRLS 660
            Q  GKN+ +        R   + +GI F SRP+   EK+ + ++ +   W+GALRFGFT H+P  +    E IP+Y CPDL+ R G + + + +   +  + + +W++  G V   VN+   V+  C +  +   +W ++DVYG T EV        +D ++  T  P   SS RLS
Sbjct:   71 QAKGKNIRLDTYGRKAIRRNSFCNGITFTSRPIHLYEKVRLKLVSVHHGWSGALRFGFTIHDPAQMK--LEDIPKYACPDLVTRPGYWAKALPERFAQRDNILAFWVDRHGRVFYSVNDEEPVLFHCGVK-VSSPLWALIDVYGITQEVQL------LDSMFADTMAPSRLSSPRLS 238
[Close]
BLAST of Neuralized, putative vs. Ensembl Xenopus
Match: ENSXETT00000038138.1 (neuralized E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:100145805])

HSP 1 Score: 70.0922 bits (170), Expect = 1.687e-12
Identity = 42/146 (28.77%), Postives = 72/146 (49.32%), Query Frame = 1 
Query:  151 GKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEV-SLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEV 579
            G ++T+ +     +R + +  GI F +RP+   EK+ I VLK    W+GALR G+T+ +P  +      +P ++CPDL      +   I +   +    I +W    G+V   +  E   +    GI K+  +W ++DVYG T  +
Sbjct:   38 GCHITLNSCLHQAERQQSFHHGIIFSNRPLLSKEKLWIRVLKEEERWHGALRIGYTSVDPSTLEPCC--LPPFVCPDLTNLPRFWGVGIPEELCQAGALIKFWFSKNGKVFCQREGEPCARVLFSGIPKNTKLWAMLDVYGKTKAI 181
[Close]
BLAST of Neuralized, putative vs. Ensembl Xenopus
Match: neurl4 (neuralized E3 ubiquitin protein ligase 4 [Source:NCBI gene;Acc:100036703])

HSP 1 Score: 67.0106 bits (162), Expect = 6.036e-11
Identity = 81/359 (22.56%), Postives = 148/359 (41.23%), Query Frame = 1 
Query:  106 KSQPVRPPM-----KIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPR-----------YMCPDLIKRGG-----SYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWPVVDVYG--NTVEVTFRSLDDNVDLIYTPT---------------EPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIKAFAWNQ------NPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEKLSDFARGNGWIFE-------NVYIRMVPGDEVAIC--------ILD-SGVVQVAVNG 1002
            KS P + P+     + H + GKN++V  D    +R  G S GI F SR +   E  EI V K+   W+G+   G T+ +P  +S    G+P             +C   + R G     +Y  ++ ++  RV  ++    +S   + + +N         GI K V+ V+D++G    +     SL +  D    P+               +P         QF L+ +  ++ L   +    +   +  ++NQ       P P   ++ + ++++     ++LSI ++ ++P+  N P   +   R + WIF+        V IR   G  + +C        +LD SG + + VNG
Sbjct:  618 KSLPSQSPVPSVSHRFHTVCGKNVSVLCDGTRAERGAGCSHGIVFSSRELLTNETFEIRVEKIEPHWSGSCNIGVTSLSPHEVSLLGGGLPERALELRSKVTWLVCGSEVTRNGQRLRENYCNSLERV--RVGSRLGVRRDSDDTLHILMNGEDMGPAACGIPKAVYAVLDIHGCITALSTVSSSLLEEPDATKPPSITSESEEEEDPADHGDPHTVIQAHSLQF-LANHGKNILLSHGN----RTATRVSSYNQGIVVLPQPLPRLFLFQIRIDQLSPHWTSSLSIGVIAVSPERLNFPATAAALKR-SSWIFQRSAVLCNGVKIREGYGPNLDLCPEGTCLGLLLDSSGGLHLYVNG 968

HSP 2 Score: 53.1434 bits (126), Expect = 1.161e-6
Identity = 51/234 (21.79%), Postives = 97/234 (41.45%), Query Frame = 1 
Query:  100 NSKSQPVRPPMKIHQIHGKNLTV-GADRLSVKRH--EGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPI------AISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVK--DKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSL--------DDNVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIKAF 744
            +S S P    +  H   G+  ++    R +++ H  + ++ G+   +RP+ + E  ++ + KM+ +W G++  G TTHNP        ++N   G        ++  G + ++       R+K  D +       G ++  VN + Q      +  +V+ VVD+YG   + T            D++  L  TP  P    SL   +F     A ++      TV +K +   F
Sbjct:  247 HSSSTPEPGQLLFHSNCGQKASIINGGRTALRPHATDDFNHGVVLSNRPLQNNEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHNLDRLKAGDTVGVVRREDGTLNFFVNGIAQGVAAWNVPPNVFAVVDLYGQAAQATIMDESDALPLPGDEDEGLALTPGTPCALLSLSDLRFHHLHGANALITNGGRTVLRKNSRSEF 480
[Close]
BLAST of Neuralized, putative vs. Ensembl Mouse
Match: Neurl1a (neuralized E3 ubiquitin protein ligase 1A [Source:MGI Symbol;Acc:MGI:1334263])

HSP 1 Score: 98.5969 bits (244), Expect = 3.539e-21
Identity = 68/256 (26.56%), Postives = 117/256 (45.70%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANA--VSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEK 879
            P+  H    G  + +     +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  H + +P+Y CPDL+ + G + + + +      + I +W++  G V  ++NE        G+  V  +W +VDVYG T  V    L D+  ++     PR +++LR       A+   +SV L D +     G+  A      P P N++       +   +  +L    L+    V  L E+
Sbjct:   62 PLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--HPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRINESAAMLFFSGVRTVDPLWALVDVYGLTRGV---QLLDSELVLPDCLRPRSFTALRRPSLRCEADEARLSVSLCDLNVPGADGDDGAPPAG-CPIPQNSLNSQHSRALPAQLDGDLRFHALRAGAHVRILDEQ 311
[Close]
BLAST of Neuralized, putative vs. Ensembl Mouse
Match: Neurl1a (neuralized E3 ubiquitin protein ligase 1A [Source:MGI Symbol;Acc:MGI:1334263])

HSP 1 Score: 98.2117 bits (243), Expect = 3.612e-21
Identity = 68/256 (26.56%), Postives = 117/256 (45.70%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANA--VSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEK 879
            P+  H    G  + +     +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  H + +P+Y CPDL+ + G + + + +      + I +W++  G V  ++NE        G+  V  +W +VDVYG T  V    L D+  ++     PR +++LR       A+   +SV L D +     G+  A      P P N++       +   +  +L    L+    V  L E+
Sbjct:   45 PLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--HPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRINESAAMLFFSGVRTVDPLWALVDVYGLTRGV---QLLDSELVLPDCLRPRSFTALRRPSLRCEADEARLSVSLCDLNVPGADGDDGAPPAG-CPIPQNSLNSQHSRALPAQLDGDLRFHALRAGAHVRILDEQ 294
[Close]
BLAST of Neuralized, putative vs. Ensembl Mouse
Match: Neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:MGI Symbol;Acc:MGI:3643092])

HSP 1 Score: 97.0561 bits (240), Expect = 9.012e-21
Identity = 60/183 (32.79%), Postives = 96/183 (52.46%), Query Frame = 1 
Query:  142 QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSA 678
            Q  GKN+ +        R   + +G+ F  RP+   E++ + ++ +   W+GALRFGFT H+P  +S  A+ IP+Y CPDL+ R G + + + + +L ++D ++ YW +  G V   VN+   V+  C +  +   +W ++DVYG T EV    L+       TP        LRL Q  LSA
Sbjct:   44 QAKGKNVRLDGHSRRATRRNSFCNGVTFTQRPIRLYEQVRLRLVAVRPGWSGALRFGFTAHDPSLMS--AQDIPKYACPDLVTRPGYWAKALPE-NLALRDTVLAYWADRHGRVFYSVNDGEPVLFHCGV-AVGGPLWALIDVYGITDEVQL--LESTFADTLTP--------LRLGQARLSA 212
[Close]
BLAST of Neuralized, putative vs. Ensembl Mouse
Match: Neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:MGI Symbol;Acc:MGI:3643092])

HSP 1 Score: 93.9745 bits (232), Expect = 1.371e-20
Identity = 55/184 (29.89%), Postives = 94/184 (51.09%), Query Frame = 1 
Query:   88 GNTPNSKSQPVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPT 627
            G  P  ++     P    Q  GKN+ +        R   + +G+ F  RP+   E++ + ++ +   W+GALRFGFT H+P  +S  A+ IP+Y CPDL+ R G + + + + +L ++D ++ YW +  G V   VN+   V+  C +  +   +W ++DVYG T EV       +     TP+
Sbjct:   26 GPGPERRAVLGEAPRFHAQAKGKNVRLDGHSRRATRRNSFCNGVTFTQRPIRLYEQVRLRLVAVRPGWSGALRFGFTAHDPSLMS--AQDIPKYACPDLVTRPGYWAKALPE-NLALRDTVLAYWADRHGRVFYSVNDGEPVLFHCGV-AVGGPLWALIDVYGITDEVQLLGTLQSSSETMTPS 205
[Close]
BLAST of Neuralized, putative vs. Ensembl Mouse
Match: Neurl1a (neuralized E3 ubiquitin protein ligase 1A [Source:MGI Symbol;Acc:MGI:1334263])

HSP 1 Score: 89.3521 bits (220), Expect = 7.287e-20
Identity = 47/159 (29.56%), Postives = 79/159 (49.69%), Query Frame = 1 
Query:  115 PVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTF 585
            P  P +      G  + +     +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  H + +P+Y CPDL+ + G + + + +      + I +W++  G V  ++NE        G+  V  +W +VDVYG T  V  
Sbjct:   59 PATPLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--HPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRINESAAMLFFSGVRTVDPLWALVDVYGLTRGVQL 215
[Close]
BLAST of Neuralized, putative vs. UniProt/SwissProt
Match: sp|P29503|NEUR_DROME (Protein neuralized OS=Drosophila melanogaster OX=7227 GN=neur PE=1 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 2.235e-21
Identity = 49/157 (31.21%), Postives = 85/157 (54.14%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEG-IPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P+ +    EG +P+Y CPDL  R G + + + +      + + Y++   G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:  105 PPLQFHSVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPVTL----EGTLPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNGAGDVIYGINNEEKGVILTGIDTRSLLWTVIDIYGNCTGIEF 257

HSP 2 Score: 60.4622 bits (145), Expect = 2.935e-8
Identity = 51/178 (28.65%), Postives = 83/178 (46.63%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPR 636
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+
Sbjct:  368 PVPFHNTKGRNVRLSQDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPAMLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQ 536
[Close]
BLAST of Neuralized, putative vs. UniProt/SwissProt
Match: sp|Q24746|NEUR_DROVI (Protein neuralized OS=Drosophila virilis OX=7244 GN=neur PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 3.072e-21
Identity = 47/156 (30.13%), Postives = 84/156 (53.85%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTF 585
            PP++ H +HG N+ +  D    +R E +   I F +RPV   E+I +   ++ + WNG +RFGFT+++P ++      +P+Y CPDL  R G + + + +      + + Y++ + G+V   +N   +   L GI     +W V+D+YGN   + F
Sbjct:   96 PPLQFHTVHGDNIRISRDGTLARRFESFCRAITFSARPVRINERICVKFAEISNNWNGGIRFGFTSNDPASLEG---ALPKYACPDLTNRPGFWAKALHEQYCEKDNILYYYVNNAGDVIYGINNEEKGVILSGIDTRGLLWTVIDIYGNCTGIEF 248

HSP 2 Score: 58.151 bits (139), Expect = 1.714e-7
Identity = 51/179 (28.49%), Postives = 83/179 (46.37%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEG-YSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPD---LIKRGGSYV--ETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLH-GIVKDVWPVVDVYGNTVEV-TFRSLDDNVDLIYTPTEPRF 639
            P+  H   G+N+ +  DR    R E  +  G  F +RP+  GEK+ + VLK    + GAL  G T+ NP  +       P  +  D   L+ R   +V  + I+    R  D+I +++   GEVS+  N       +H      +W  +DVYG+T  +  FR    N  ++  P++P+ 
Sbjct:  359 PVPFHITKGRNVRLSHDRFVASRTESDFCQGYVFTARPIRIGEKLIVQVLKTEQMYVGALALGLTSCNPALLQ------PNDLPNDSDFLLDRPEYWVVSKDIAAAPQR-GDEIAFFVAPNGEVSISKNNGPAVVVMHVDQSLQLWAFLDVYGSTQSLRMFRQQLPN--MVAYPSQPQI 528
[Close]
BLAST of Neuralized, putative vs. UniProt/SwissProt
Match: sp|Q923S6|NEUL1_MOUSE (E3 ubiquitin-protein ligase NEURL1 OS=Mus musculus OX=10090 GN=Neurl1 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.475e-20
Identity = 68/256 (26.56%), Postives = 117/256 (45.70%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANA--VSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEK 879
            P+  H    G  + +     +VKR   + + I F +RPV   E++ + + K    W+GALR GFT+ +P  I  H + +P+Y CPDL+ + G + + + +      + I +W++  G V  ++NE        G+  V  +W +VDVYG T  V    L D+  ++     PR +++LR       A+   +SV L D +     G+  A      P P N++       +   +  +L    L+    V  L E+
Sbjct:   62 PLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--HPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRINESAAMLFFSGVRTVDPLWALVDVYGLTRGV---QLLDSELVLPDCLRPRSFTALRRPSLRCEADEARLSVSLCDLNVPGADGDDGAPPAG-CPIPQNSLNSQHSRALPAQLDGDLRFHALRAGAHVRILDEQ 311
[Close]
BLAST of Neuralized, putative vs. UniProt/SwissProt
Match: sp|A8MQ27|NEU1B_HUMAN (E3 ubiquitin-protein ligase NEURL1B OS=Homo sapiens OX=9606 GN=NEURL1B PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.894e-20
Identity = 69/231 (29.87%), Postives = 118/231 (51.08%), Query Frame = 1 
Query:   82 IMGNTPNSKSQPVRPPM-----KIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTP---TEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNI 735
            ++   P     P R P+     + H Q  GKN+ +        R   + +G+ F  RP+   E++ + ++ +   W+GALRFGFT H+P  +S  A+ IP+Y CPDL+ R G + + + + +L ++D ++ YW +  G V   VN+   V+  C +  +   +W ++DVYG T EV    L+       TP   ++ RF + L  S    +AN  + EL ++  V K G++
Sbjct:   18 LLATRPCCGPGPERRPVLGEAPRFHAQAKGKNVRLDGHSRRATRRNSFCNGVTFTQRPIRLYEQVRLRLVAVRPGWSGALRFGFTAHDPSLMS--AQDIPKYACPDLVTRPGYWAKALPE-NLALRDTVLAYWADRHGRVFYSVNDGEPVLFHCGV-AVGGPLWALIDVYGITDEVQL--LESAFADTLTPARLSQARFSACLPPSSHD-AANFDNNELENNQVVAKLGHL 241
[Close]
BLAST of Neuralized, putative vs. UniProt/SwissProt
Match: sp|Q0MW30|NEU1B_MOUSE (E3 ubiquitin-protein ligase NEURL1B OS=Mus musculus OX=10090 GN=Neurl1b PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.464e-20
Identity = 60/183 (32.79%), Postives = 96/183 (52.46%), Query Frame = 1 
Query:  142 QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSA 678
            Q  GKN+ +        R   + +G+ F  RP+   E++ + ++ +   W+GALRFGFT H+P  +S  A+ IP+Y CPDL+ R G + + + + +L ++D ++ YW +  G V   VN+   V+  C +  +   +W ++DVYG T EV    L+       TP        LRL Q  LSA
Sbjct:   44 QAKGKNVRLDGHSRRATRRNSFCNGVTFTQRPIRLYEQVRLRLVAVRPGWSGALRFGFTAHDPSLMS--AQDIPKYACPDLVTRPGYWAKALPE-NLALRDTVLAYWADRHGRVFYSVNDGEPVLFHCGV-AVGGPLWALIDVYGITDEVQL--LESTFADTLTP--------LRLGQARLSA 212
[Close]
BLAST of Neuralized, putative vs. TrEMBL
Match: A0A4Y2H5K2 (E3 ubiquitin-protein ligase NEURL1 OS=Araneus ventricosus OX=182803 GN=Neurl1 PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 2.664e-32
Identity = 121/474 (25.53%), Postives = 192/474 (40.51%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFRS------------------LDDNVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKG-NIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNN---LPEKLSDFA-RGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            P+  HQ+HG+N+ +  +     R EG+  GI F +RPV  GE I I  L++ + W+GALR GFT ++P+ + +    +PRY CPDL    G++ + I++   +   K+ Y+++ +G+V   +N V       G+     +W V+D+YGNTV V F +                  +  N D++ T T   F  +  LS   +    VS++   +    K G N  A+ + +          + ++  +     NL   I    P   N   LPE   D   R   WI  N       GD +   I   G V V  N +    +  V P    W+  +M  S+  +  +G                           S++P  D       +   I       + E     P    +     ECVIC     D     CGH+C C +CA  L N   +  CP CR+ I+   ++Y
Sbjct:   32 PLHFHQVHGENIKLLKNGAVACRSEGFCKGIAFSNRPVMVGEPIYIKFLEISTNWSGALRIGFTMNDPMTMKSK---LPRYACPDLTSIQGNWAKAIAERMAKKDSKLYYYVDHSGDVHYGLNGVEHGVFFGGVNTSGKLWAVLDIYGNTVAVEFVNPHSMNPECAVEASQNMSLVPKNKDVMSTST-LNFQINQHLSFHCVHGKNVSLKANKTVASRKYGFNSLAYVFTEYSLKLEEKIYIRISRTNPKFNGNLLYGITSCNPSTLNPHVLPENADDLMDRPEYWIVRNDTNSYDAGDVIVYSISAGGEVTVMKNCDYKSAIFHVDPTQPLWLFFNMIGSVSEINILG---------------------------SSVP--DSALSDVADFDEIMSVSLPLKTEKANGMPGTSGQQTCN-ECVICFGEQVDCALYRCGHMCMCYSCASKLKNS-ANDQCPICREVIQDVIRIY 470
[Close]
BLAST of Neuralized, putative vs. TrEMBL
Match: A0A2L2YNI6 (Neuralized-like protein 1A OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 4.592e-32
Identity = 118/474 (24.89%), Postives = 195/474 (41.14%), Query Frame = 1 
Query:  127 PMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTF----RSLDD------------NVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIK-AFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEKLSDFA-----RGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAAS-ALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            P+  HQ+HG N+ +  +     RHEG+  GI F  RPV  GE + I  +++ S W+GALR GFT ++PI++ +    +PRY CPDL    G++ + I++   +    + Y+++  G+V   ++         G+   + +W V+D+YGNT  V F     S+ D            N D   + TE  F    R+S  T       ++        KKG+   ++ +   P        + V  ++       +  I    P  + LP++L   A     R   W+  N        D +   I + G VQV  N  R      V      W+ ++++  +  +  +G            P        D  G  +  LPS     P+  EI   P  E++N  ++                CV+C + + +     CGHVC C  CA  + N   ++ CP CR+ I    K+Y
Sbjct:   31 PLHFHQVHGDNIKLMKNGTVACRHEGFCKGIAFSDRPVLVGEPVYIKFIEISSNWSGALRIGFTMNDPISMKSK---LPRYACPDLTSINGNWAKAIAERMAQKDSILFYYVDQNGDVHYGIDSEEHGIFFGGVNTSEKLWAVLDIYGNTTAVEFIEPRSSIQDFISESDHNIIPVNSDFQLSHTENSFIVGSRMSFHTTHGKNAVLKRYRLVASRKKGSSNLSYVFTDRPLNIKEKLQIKVINIEPQASGTFNYGITSCNPS-SLLPDELPPLAEALVDRLEYWVVRNDTNAYKIHDIITFSINEEGEVQVLKNSVRSSRF-HVDSTLPLWVFLNLSGIVSEVRIIG-----------SPDAYECDITDQFGKLTVTLPS---NIPSMDEIAG-PSSEDTNSDKI----------------CVVCFEELANCALYRCGHVCMCYDCAHKMWNPGSNVQCPICREPIRDVIKIY 468
[Close]
BLAST of Neuralized, putative vs. TrEMBL
Match: A0A3S3PAW9 (Protein neuralized-like protein OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_11436 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.543e-29
Identity = 114/488 (23.36%), Postives = 210/488 (43.03%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHG--IVKDVWPVVDVYGNTVEVTF---RSLDDNV---DLIYTPTE--------------PRFYSSLRLSQFTL-SANAVSVELVDSDTVCKKGN---IKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQV---NNLPEKLSD-FARGNGW-IFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVG----LYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESN--RQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM 1476
            PP++ H +HG+N+ + +D    +R E +  GI F +RP+   E++ +  + + S W+GA+RFGFT ++P    N+   +P+Y CPDL  R G++ + +++   +    + ++    GEV   ++   +        I   +W ++DVYGNT  +     RS   NV   D+ +  ++              P+ Y ++ L+          +V L +   + ++ +    + + + Q P       V  + + D     +L+  +    P     N+LPE       R   W I ++V    V GDE+A+ I D+G V++  N +    L  V      +   D+  S I +  +G    ++ M++  L  +      L L    ++S L               +P++++SN   Q    +N           EC +C +   ++V   CGH+C C  CA       G   CP CR  I+   + Y+
Sbjct:   18 PPIRFHSVHGENIRITSDGAIARRVESFCKGITFSNRPIRVNERVYLKFVDISSNWSGAVRFGFTNNDPNTCRNN---LPKYACPDLRNRPGNWAKALAERLAQRDSTLYFYYNEEGEVHYNIDGEDKGVFFSDMQISPQMWAIIDVYGNTCALELVDPRSNSGNVRTRDMNHLVSQLSNSSISRENREPLPQIYCNVALNPILFHRTKGCNVRLTNDRCIAERNSSHYCQGYVFTQRPLNLEEKLVFQILQTDGLYTGSLAFGLTTCNPSSINGNDLPEDAHQLLERPEYWVIIKDVATAPVAGDELAVKICDNGEVKLYKNRQPPTTLMHVDQTQKLYAFFDLYGSTIKIKLLGTIIDIHNMSQSQLNHILPGYQPLRLLSETSSSNLSVC------------LPLKDKSNCASQHGSESNSNAIN----LGECTVCYEKPINSVLYICGHMCMCYECAFKQWKGTGGGQCPICRAMIQDVIRTYV 486
[Close]
BLAST of Neuralized, putative vs. TrEMBL
Match: A0A3S4RI89 (Protein neuralized-like protein OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_05780 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 7.280e-29
Identity = 114/488 (23.36%), Postives = 210/488 (43.03%), Query Frame = 1 
Query:  124 PPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHG--IVKDVWPVVDVYGNTVEVTF---RSLDDNV---DLIYTPTE--------------PRFYSSLRLSQFTL-SANAVSVELVDSDTVCKKGN---IKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQV---NNLPEKLSD-FARGNGW-IFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVG----LYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESN--RQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM 1476
            PP++ H +HG+N+ + +D    +R E +  GI F +RP+   E++ +  + + S W+GA+RFGFT ++P    N+   +P+Y CPDL  R G++ + +++   +    + ++    GEV   ++   +        I   +W ++DVYGNT  +     RS   NV   D+ +  ++              P+ Y ++ L+          +V L +   + ++ +    + + + Q P       V  + + D     +L+  +    P     N+LPE       R   W I ++V    V GDE+A+ I D+G V++  N +    L  V      +   D+  S I +  +G    ++ M++  L  +      L L    ++S L               +P++++SN   Q    +N           EC +C +   ++V   CGH+C C  CA       G   CP CR  I+   + Y+
Sbjct:   30 PPIRFHSVHGENIRITSDGAIARRVESFCKGITFSNRPIRVNERVYLKFVDISSNWSGAVRFGFTNNDPNTCRNN---LPKYACPDLRNRPGNWAKALAERLAQRDSTLYFYYNEEGEVHYNIDGEDKGVFFSDMQISPQMWAIIDVYGNTCALELVDPRSNSGNVRTRDMNHLVSQLSNSSISRENREPLPQIYCNVALNPILFHRTKGCNVRLTNDRCIAERNSSHYCQGYVFTQRPLNLEEKLVFQILQTDGLYTGSLAFGLTTCNPSSINGNDLPEDAHQLLERPEYWVIIKDVATAPVAGDELAVKICDNGEVKLYKNRQPPTTLMHVDQTQKLYAFFDLYGSTIKIKLLGTIIDIHNMSQSQLNHILPGYQPLRLLSETSSSNLSVC------------LPLKDKSNCASQHGSESNSNAIN----LGECTVCYEKPINSVLYICGHMCMCYECAFKQWKGTGGGQCPICRAMIQDVIRTYV 498
[Close]
BLAST of Neuralized, putative vs. TrEMBL
Match: H2YX88 (Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 8.625e-27
Identity = 122/507 (24.06%), Postives = 203/507 (40.04%), Query Frame = 1 
Query:  130 MKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKD--VWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLS--ANAVSVELVDSDTVCKKG----------------NIKAFAWNQNPFPTNTIWVLFVNEVDH--------SIG----NNLSIAILKLAPQVNNLPEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEE-LGKVSPNSFYWIAVDMNCSLINLTSVG--------------------------LYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
            +  H IHGKN+T+       +R   +  GI F SRP+   E   + + ++   W+G +RFG T   P  +   ++G+PR++CPDL  + G + + +S         I + +  +GE+   VN   Q+  + GI  +  +W V+DVYGN+  + F        LI    EP   +  RL Q   S  ++    +LVD    C  G                N  +  ++   F     W L V + +H         +G    N  SI +   + + N+L ++         W+ ++V+     GD  +I I D G V   V     EE L     +   WI +DM  SL +L SV                           ++ ++        +  +++        + + + D   P++     I    ++   + P N P K  E      C +C+D   +     CGHVC C+AC+  L+       CP CRK I+   K+Y
Sbjct:    4 LTFHNIHGKNITLSEGNKVAQRINSFCYGIVFSSRPIRINEVAHLKLEQISHMWSGLVRFGVTCIRPEILG--SQGLPRFLCPDLTNQDGIWAKALSDEFANENTVISFHVNHSGELCYMVNGTSQESIISGIPTEQPLWVVMDVYGNSTALKFVG---KWTLILYELEPDENNLRRLRQLISSDFSDWDLNDLVDIGFSCHTGKNIRLSSNNVYKIRTTNKHSIVFSDRAFRVEDTWHLKVGQSEHGDFDVPVLGVGVTNCNPESIDMESWSGETNDLLDRFE------YWVLQDVFPCPRKGDFFSITITDDGFVSFKVLNSGLEEKLFFCDTSQPLWIVIDMRGSLRHLVSVAGSALDGVSSTLWVVVEGDGSVSNNIWMFMVSVVIYSETSAHRSLVNNTALLETTIMSTND--APSSSGAAGITPDAKNKAGKSP-NPPLKDSE------CSLCVDAPANIAVYDCGHVCLCEACSKKLLQVERFPKCPICRKMIKDVMKIY 490
[Close]
BLAST of Neuralized, putative vs. Ensembl Cavefish
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:NCBI gene;Acc:103031899])

HSP 1 Score: 95.1301 bits (235), Expect = 2.779e-20
Identity = 110/518 (21.24%), Postives = 190/518 (36.68%), Query Frame = 1 
Query:  100 NSKSQPVRPPMKIHQIH--GKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNE---VMQKCQL------------HGIVKDVWPVVDVYGNTVEVTFRSLDD-NVDLIYTPTEPRFYSSLRL-SQFTLSANAVSVELVDSDTVCKKGNI------------------------------------KAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEKLSD----FARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRA-ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVY 1473
             S  QP  P       H  GKN+ + A      R   + +GI F  RPV   EK+ + +  + + W+GALRFGFT+ +P  ++  +  IP+Y CPDL+ R G + + + +      + + +W +  G V   +NE   V+  C L            +GI ++V  +   +  +V  +  S    +  L  + T+   YS+ +L S    +A   +++L   + V + G +                                    +   ++  P        L V  +       L   +    P   +  E  +D      R   W+         PGD ++  +L SG V   VNG     L  V  +   W+   ++ ++  L  +G  + +    P +       + D     S L     +  +  E   +          L  +      +   +  EC +C D   D V   CGH+C C  C   L  +  +  CP CR+ I+   K Y
Sbjct:   11 TSTQQPSNPESPRFHPHAKGKNIRLDAQLRRAARRNSFCNGITFSQRPVRLYEKLRLRLSGVHTGWSGALRFGFTSLDPGDLT--STDIPKYACPDLVTRPGYWAKALPERLASRDNVLSFWADRHGRVFYSINEAEPVLFHCGLSVSDPLWAIIDIYGITQEVTLLESSFAESVGTSCLSAARLSAYLPQSNTDSANYSNNQLESSQAAAAKMATLQLAGYNHVQRTGRVPLPALDTDLHFHPVRGTDVVLSTDRAAACVHFLDSSRTLVFSDRPLRVGETLYLEVGHLGLPYFGALLFGMTSCDPGTLHAGELPADPDVLLDRKEYWVVYRGLPVPGPGDVLSFTLLSSGEVHHGVNGTSRGRLLCVDSSQVLWVFFALHGAVNRLRILGTLQASPSTSPSISRGTASDDSD-----SDLAFSVNRSSSASESSLVTAPSSPLSPPLSPSLSLPETQRGCKGGECTVCFDQEVDTVIYTCGHLCLCNDCGLKLKRQINAC-CPICRRPIKDVIKTY 520
[Close]
BLAST of Neuralized, putative vs. Ensembl Cavefish
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:NCBI gene;Acc:103031899])

HSP 1 Score: 92.8189 bits (229), Expect = 1.626e-19
Identity = 53/167 (31.74%), Postives = 85/167 (50.90%), Query Frame = 1 
Query:  100 NSKSQPVRPPMKIHQIH--GKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTF 585
             S  QP  P       H  GKN+ + A      R   + +GI F  RPV   EK+ + +  + + W+GALRFGFT+ +P  ++  +  IP+Y CPDL+ R G + + + +      + + +W +  G V   +NE   V+  C L  +   +W ++D+YG T EVT 
Sbjct:   39 TSTQQPSNPESPRFHPHAKGKNIRLDAQLRRAARRNSFCNGITFSQRPVRLYEKLRLRLSGVHTGWSGALRFGFTSLDPGDLT--STDIPKYACPDLVTRPGYWAKALPERLASRDNVLSFWADRHGRVFYSINEAEPVLFHCGL-SVSDPLWAIIDIYGITQEVTL 202
[Close]
BLAST of Neuralized, putative vs. Ensembl Cavefish
Match: neurl1aa (neuralized E3 ubiquitin protein ligase 1Aa [Source:ZFIN;Acc:ZDB-GENE-090311-3])

HSP 1 Score: 87.8113 bits (216), Expect = 6.447e-18
Identity = 56/205 (27.32%), Postives = 103/205 (50.24%), Query Frame = 1 
Query:  115 PVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFRSLDDNV---DLIYTPTEPRFYSSLR-LSQFTLSANA--VSVELVD 705
            PV P +      G  + +   + +VKR   + + I F +RP+   E++ + + K    W+GALR GFT+ +P  I  + + +P+Y CPDL+ + G + + + +      + + +W++  G V  ++N+        G+  V+ +W ++DVYG T  V    LD  +   D +     PR ++++R  S     A+A  +SV L D
Sbjct:    4 PVSPLLFHPSAKGSQIVMDLAQRTVKRQASFCNAITFSNRPIALYEQVRLKITKKQCCWSGALRLGFTSKDPSRI--NPDSLPKYACPDLVSQSGFWAKALPEEFANEGNLVAFWVDKKGRVFYRINDSSPMLFFSGVRTVEPLWALIDVYGLTRGVQL--LDSEIVPPDCL----RPRSFTTVRGGSSLRREADASRLSVSLCD 200
[Close]
BLAST of Neuralized, putative vs. Ensembl Cavefish
Match: ENSAMXT00000001187.2 (protein neuralized-like [Source:NCBI gene;Acc:103023442])

HSP 1 Score: 63.5438 bits (153), Expect = 1.333e-10
Identity = 38/153 (24.84%), Postives = 76/153 (49.67%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGAD-RLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIV---KDVWPVVDVYGNT 570
            PM  H +  G+ +T+  D R + +    +  G+ F SRP+   EK+ + + + +  W+GALR GFT+  P      +  +P    P+L    G +   + +       ++++W+  +G + ++  +  Q+ ++   V   K +W ++DVYG T
Sbjct:   24 PMTFHSEAKGQLVTLSEDGRRATRDTSSFRHGLIFSSRPLRVQEKVCVRIERCVMGWHGALRVGFTSVAP-----GSRAVPSLAIPELTDFPGYWAIPVPEQQCLPGSEVIFWIGPSGSLHVQTAD-GQRHRMKTRVDKHKPIWAMIDVYGQT 170

HSP 2 Score: 49.2914 bits (116), Expect = 6.041e-6
Identity = 53/218 (24.31%), Postives = 90/218 (41.28%), Query Frame = 1 
Query:  826 LSIAILKLAPQVNNLPE----KLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQV-AVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQ----ELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKI 1452
            L +    +AP    +P     +L+DF     W       + +PG EV   I  SG + V   +G+R     +V  +   W  +D+      +  +G  K   +       +P +   +     S +P    +R   +      + E+ NR+       ++N  K   Y+   ECV+C   I D V + CGH C C  CA  +++E G+  CP CR ++
Sbjct:   84 LRVGFTSVAPGSRAVPSLAIPELTDFP--GYWAIPVPEQQCLPGSEVIFWIGPSGSLHVQTADGQRHRMKTRVDKHKPIWAMIDVYGQTNTVLLLGSMKKCFFSTRRSCPVPTVEPTEETCGYSNVPIEILERMMNQN----SLEEQENRKNDNLSCCQSN-RKSTGYESMEECVVCYSDIAD-VMLTCGHRCVCSHCAWRVLSEFGT--CPLCRYRL 291
[Close]
BLAST of Neuralized, putative vs. Ensembl Cavefish
Match: ENSAMXT00000056033.1 (protein neuralized-like [Source:NCBI gene;Acc:103023442])

HSP 1 Score: 62.7734 bits (151), Expect = 1.814e-10
Identity = 38/153 (24.84%), Postives = 76/153 (49.67%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGAD-RLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIV---KDVWPVVDVYGNT 570
            PM  H +  G+ +T+  D R + +    +  G+ F SRP+   EK+ + + + +  W+GALR GFT+  P      +  +P    P+L    G +   + +       ++++W+  +G + ++  +  Q+ ++   V   K +W ++DVYG T
Sbjct:   24 PMTFHSEAKGQLVTLSEDGRRATRDTSSFRHGLIFSSRPLRVQEKVCVRIERCVMGWHGALRVGFTSVAP-----GSRAVPSLAIPELTDFPGYWAIPVPEQQCLPGSEVIFWIGPSGSLHVQTAD-GQRHRMKTRVDKHKPIWAMIDVYGQT 170
[Close]
BLAST of Neuralized, putative vs. Ensembl Sea Lamprey
Match: si:dkey-49c17.3 (ring finger protein 157 [Source:HGNC Symbol;Acc:HGNC:29402])

HSP 1 Score: 50.447 bits (119), Expect = 7.267e-7
Identity = 22/47 (46.81%), Postives = 28/47 (59.57%), Query Frame = 1 
Query: 1303 DFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCR 1443
            D   ECV+CL  I D + +PC H+C C ACAD L  +  +  CP CR
Sbjct:  272 DNSTECVVCLSDIRDTLILPCRHLCLCSACADTLRYQANN--CPICR 316
[Close]
BLAST of Neuralized, putative vs. Ensembl Nematostella
Match: EDO31830 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWU1])

HSP 1 Score: 57.3806 bits (137), Expect = 2.885e-9
Identity = 27/93 (29.03%), Postives = 48/93 (51.61%), Query Frame = 1 
Query:  130 MKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYV 408
            M+ H  HG ++T+   +    R + +++ + F +RP+   E     + +    W+G +R G TTHNP  I      +P Y+ PDL + G ++V
Sbjct:    2 MRFHPKHGSHITLSPCQTVATRCKSFANAVVFSNRPLSTSETFVFEIYEQEQGWSGHVRCGVTTHNPNFIK-----VPPYLLPDLAQMGTTWV 89
[Close]
BLAST of Neuralized, putative vs. Ensembl Nematostella
Match: EDO43423 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYU6])

HSP 1 Score: 58.151 bits (139), Expect = 5.956e-9
Identity = 25/55 (45.45%), Postives = 33/55 (60.00%), Query Frame = 1 
Query: 1315 ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSI-LCPTCRKKIEHSYKVYM 1476
            ECVICLD   D V +PCGHVCCC  CA       G++  CP CR+ +    ++Y+
Sbjct:  697 ECVICLDNRSDVVMLPCGHVCCCSNCA-------GAVSACPICRQTLSQRVRMYI 744
[Close]
BLAST of Neuralized, putative vs. Ensembl Nematostella
Match: EDO44784 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUP8])

HSP 1 Score: 52.7582 bits (125), Expect = 6.091e-8
Identity = 40/162 (24.69%), Postives = 77/162 (47.53%), Query Frame = 1 
Query:  148 HGKNLTVGADRLSVKRH-EGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNP----IAISNHAEGIPRYMCPDLIKRGGSYVET--------ISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGI--VKDVWPVVDVYGNTVEVTFR 588
            HG+ + +  +  S  R  + Y  G+ +  RP+  GE  ++ + ++ ++W G+LR G T  NP      +      +    C D     GS V          ++  SL+ KD+I   + + G++   VN + +   + G+   K+++ + DVYG T EV+++
Sbjct:    8 HGRFIKLSKNFTSATREGDTYDYGVVYSDRPIHLGEVFQLQIDQVETKWAGSLRIGVTLKNPNCPQTVVVRAVTDLFVEDCKDYWILSGSRVHNGSYEKDSDLNVHSLKQKDRIGVQVTAKGDLVFFVNGICKGIAMTGLPTKKELFVIFDVYGRTKEVSWK 169
[Close]
BLAST of Neuralized, putative vs. Ensembl Nematostella
Match: EDO39600 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9L9])

HSP 1 Score: 55.0694 bits (131), Expect = 8.181e-8
Identity = 40/158 (25.32%), Postives = 75/158 (47.47%), Query Frame = 1 
Query:  151 GKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYV------ETISQ--ISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWP-----VVDVYGNTVEVTF 585
            G+++ +   R+S +R   Y+ G+     P+ H    +I V K+ S W+G+L  G T H+P  ++  +      +CP ++     Y+      E + +    LRV D +   ++++  + + VN   Q     G+V +  P     VVD+YG+  EV+ 
Sbjct: 1046 GQHVRLDNRRVSARRVASYNYGVVLSDVPLLHNALFQIRVTKLESHWSGSLMVGLTDHDPECLNLPSTANSMKICPWIVVNKAVYINGNKVFEGLGRGLDDLRVGDTVGLMIDTSARLHVFVNGEDQ-----GVVAEDLPAKPHMVVDLYGSCQEVSI 1198

HSP 2 Score: 53.5286 bits (127), Expect = 2.096e-7
Identity = 40/147 (27.21%), Postives = 66/147 (44.90%), Query Frame = 1 
Query:  199 EGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAI------SNHAEGIPRYMCPDLIKRGGSYVETISQISL---RVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDL 612
            E + + +   ++P+   E  E+ V K+   W G+L  G T+HNP+ I      ++   G   +    +I  G       S++SL   +  DK+     + G VS  +N V        I   V+ VVD+YG  V V+  S D + +L
Sbjct:  214 EEFKNSVVLTNQPLRDNELFEVVVEKLTDMWAGSLEVGITSHNPLQIVLPPTMTSMTSGAWMWSGTSIIINGQEVRSDYSELSLESIKAGDKVGILKTADGTVSFYLNGVCMGVAADDIPPGVFGVVDLYGAAVRVSVVSADTSNEL 360
[Close]
BLAST of Neuralized, putative vs. Ensembl Nematostella
Match: EDO38028 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE55])

HSP 1 Score: 52.373 bits (124), Expect = 3.194e-7
Identity = 22/43 (51.16%), Postives = 28/43 (65.12%), Query Frame = 1 
Query: 1315 ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCR 1443
            ECVIC+    D + +PC H+C CKACAD+L   + S  CP CR
Sbjct:  266 ECVICMSDFRDTLILPCRHLCLCKACADSL--RYQSSTCPICR 306
[Close]
BLAST of Neuralized, putative vs. Ensembl Medaka
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:NCBI gene;Acc:101173555])

HSP 1 Score: 90.1225 bits (222), Expect = 1.141e-18
Identity = 51/158 (32.28%), Postives = 86/158 (54.43%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTF 585
            P++ H +  GKN+ +        R   + +GI F  RP+   EK+ + +  + + W+GALRFGFT+ +P  +   A  IP+Y CPDL+ R G + + + +  L VKD ++ +W +  G V   +N+   ++  C L  I   +W ++D+YG T EV  
Sbjct:   49 PLRFHPRAKGKNIRLDGQLRRATRKNSFCNGITFSHRPIHLYEKVRLRLTGVHNGWSGALRFGFTSLDPNDLV--AADIPKYACPDLVTRPGYWAKALPE-RLAVKDNVLSFWADRHGRVFYNINDGEPILFHCGL-SIGCPLWAIIDIYGITQEVIL 202
[Close]
BLAST of Neuralized, putative vs. Ensembl Medaka
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:NCBI gene;Acc:101173555])

HSP 1 Score: 90.1225 bits (222), Expect = 1.265e-18
Identity = 51/158 (32.28%), Postives = 86/158 (54.43%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEVTF 585
            P++ H +  GKN+ +        R   + +GI F  RP+   EK+ + +  + + W+GALRFGFT+ +P  +   A  IP+Y CPDL+ R G + + + +  L VKD ++ +W +  G V   +N+   ++  C L  I   +W ++D+YG T EV  
Sbjct:   48 PLRFHPRAKGKNIRLDGQLRRATRKNSFCNGITFSHRPIHLYEKVRLRLTGVHNGWSGALRFGFTSLDPNDLV--AADIPKYACPDLVTRPGYWAKALPE-RLAVKDNVLSFWADRHGRVFYNINDGEPILFHCGL-SIGCPLWAIIDIYGITQEVIL 201
[Close]
BLAST of Neuralized, putative vs. Ensembl Medaka
Match: neurl1b (neuralized E3 ubiquitin protein ligase 1B [Source:NCBI gene;Acc:101173555])

HSP 1 Score: 90.1225 bits (222), Expect = 1.284e-18
Identity = 51/156 (32.69%), Postives = 86/156 (55.13%), Query Frame = 1 
Query:  127 PMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIV-YWLESTGEVSLKVNE---VMQKCQLHGIVKDVWPVVDVYGNTVEV 579
            P++ H +  GKN+ +        R   + +GI F  RP+   EK+ + +  + + W+GALRFGFT+ +P  +   A  IP+Y CPDL+ R G + + + +  L VKD ++ +W +  G V   +N+   ++  C L  I   +W ++D+YG T EV
Sbjct:   71 PLRFHPRAKGKNIRLDGQLRRATRKNSFCNGITFSHRPIHLYEKVRLRLTGVHNGWSGALRFGFTSLDPNDLV--AADIPKYACPDLVTRPGYWAKALPE-RLAVKDNVLSFWADRHGRVFYNINDGEPILFHCGL-SIGCPLWAIIDIYGITQEV 222
[Close]
BLAST of Neuralized, putative vs. Ensembl Medaka
Match: ENSORLT00000007585.2 (E3 ubiquitin-protein ligase NEURL1 [Source:NCBI gene;Acc:101171274])

HSP 1 Score: 88.5817 bits (218), Expect = 4.212e-18
Identity = 52/182 (28.57%), Postives = 92/182 (50.55%), Query Frame = 1 
Query:  118 VRPPMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHG--IVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLR 654
            V  P+  H +  G  + +   + +VKR   + +GI F +RP+   E++ + + K    W+GALR GFT+ +P  +    E +P+Y CPDL+ + G + + + +      + I +W++  G V  K+N+        G  I + +W ++DVYG T E+    L D   +      PR ++S R
Sbjct:   39 VSGPLLFHPRTKGSQILMDLSQRTVKRQSSFCNGITFSNRPIAVYEQVRLKITKKQYCWSGALRLGFTSKDPSRM--QPENLPKYSCPDLVSQCGFWAKALPEELSEEGNIISFWVDKKGRVFYKLNDSHSTQFFSGVRICEPLWALIDVYGLTKEI---QLLDTEMVAVNFMRPRSFTSER 215
[Close]
BLAST of Neuralized, putative vs. Ensembl Medaka
Match: ENSORLT00000027315.1 (E3 ubiquitin-protein ligase NEURL1 [Source:NCBI gene;Acc:101171274])

HSP 1 Score: 88.5817 bits (218), Expect = 4.212e-18
Identity = 52/182 (28.57%), Postives = 92/182 (50.55%), Query Frame = 1 
Query:  118 VRPPMKIH-QIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHG--IVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLR 654
            V  P+  H +  G  + +   + +VKR   + +GI F +RP+   E++ + + K    W+GALR GFT+ +P  +    E +P+Y CPDL+ + G + + + +      + I +W++  G V  K+N+        G  I + +W ++DVYG T E+    L D   +      PR ++S R
Sbjct:   39 VSGPLLFHPRTKGSQILMDLSQRTVKRQSSFCNGITFSNRPIAVYEQVRLKITKKQYCWSGALRLGFTSKDPSRM--QPENLPKYSCPDLVSQCGFWAKALPEELSEEGNIISFWVDKKGRVFYKLNDSHSTQFFSGVRICEPLWALIDVYGLTKEI---QLLDTEMVAVNFMRPRSFTSER 215
[Close]
BLAST of Neuralized, putative vs. Planmine SMEST
Match: SMESG000038756.1 (SMESG000038756.1)

HSP 1 Score: 917.146 bits (2369), Expect = 0.000e+0
Identity = 458/464 (98.71%), Postives = 460/464 (99.14%), Query Frame = 1 
Query:   85 MGNTPNSKSQPVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYXXXXXXXXXXXXXXDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM 1476
            MGNTPNSKSQPVRPPMKIHQIHGKNLTV ADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGN KAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYK+TRYPLPILPSIPNILELDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPT+ RE DFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM
Sbjct:    1 MGNTPNSKSQPVRPPMKIHQIHGKNLTVAADRLSVKRHEGYSDGIFFGSRPVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDLIKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVKDVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANAVSVELVDSDTVCKKGNKKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSIAILKLAPQVNNLPEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVVQVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKITRYPLPILPSIPNILELDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNPTRLRELDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM 464
[Close]
BLAST of Neuralized, putative vs. Planmine SMEST
Match: SMESG000023072.1 (SMESG000023072.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.378e-7
Identity = 28/81 (34.57%), Postives = 44/81 (54.32%), Query Frame = 1 
Query: 1315 ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM**FHLG*ISIKLVGLIIFVMNKLVYYN 1557
            +CV+CLD   + VF+PCGH+  C  C+D + N      CP CR +I+   K +   F    I+I L+ ++ F   ++V  N
Sbjct:  335 QCVVCLDKPVEVVFLPCGHLKTCVDCSDLMYN------CPVCRVRIDKRVKKFK--FRRSTINIDLISIVYFYGTRMVLEN 407
[Close]
BLAST of Neuralized, putative vs. Planmine SMEST
Match: SMESG000023072.1 (SMESG000023072.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.554e-7
Identity = 28/81 (34.57%), Postives = 44/81 (54.32%), Query Frame = 1 
Query: 1315 ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM**FHLG*ISIKLVGLIIFVMNKLVYYN 1557
            +CV+CLD   + VF+PCGH+  C  C+D + N      CP CR +I+   K +   F    I+I L+ ++ F   ++V  N
Sbjct:  324 QCVVCLDKPVEVVFLPCGHLKTCVDCSDLMYN------CPVCRVRIDKRVKKFK--FRRSTINIDLISIVYFYGTRMVLEN 396
[Close]
BLAST of Neuralized, putative vs. Planmine SMEST
Match: SMESG000023072.1 (SMESG000023072.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.784e-7
Identity = 28/81 (34.57%), Postives = 44/81 (54.32%), Query Frame = 1 
Query: 1315 ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM**FHLG*ISIKLVGLIIFVMNKLVYYN 1557
            +CV+CLD   + VF+PCGH+  C  C+D + N      CP CR +I+   K +   F    I+I L+ ++ F   ++V  N
Sbjct:  352 QCVVCLDKPVEVVFLPCGHLKTCVDCSDLMYN------CPVCRVRIDKRVKKFK--FRRSTINIDLISIVYFYGTRMVLEN 424
[Close]
BLAST of Neuralized, putative vs. Planmine SMEST
Match: SMESG000023072.1 (SMESG000023072.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.820e-7
Identity = 28/81 (34.57%), Postives = 44/81 (54.32%), Query Frame = 1 
Query: 1315 ECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCPTCRKKIEHSYKVYM**FHLG*ISIKLVGLIIFVMNKLVYYN 1557
            +CV+CLD   + VF+PCGH+  C  C+D + N      CP CR +I+   K +   F    I+I L+ ++ F   ++V  N
Sbjct:  341 QCVVCLDKPVEVVFLPCGHLKTCVDCSDLMYN------CPVCRVRIDKRVKKFK--FRRSTINIDLISIVYFYGTRMVLEN 413
The following BLAST results are available for this feature:
BLAST of Neuralized, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NEURL1B7.334e-2129.87neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
NEURL1B1.136e-2029.69neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
NEURL12.162e-2026.17neuralized E3 ubiquitin protein ligase 1 [Source:H... [more]
NEURL12.342e-1828.66neuralized E3 ubiquitin protein ligase 1 [Source:H... [more]
NEURL38.688e-1728.09neuralized E3 ubiquitin protein ligase 3 [Source:H... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
F10D7.55.724e-1626.92pep chromosome:WBcel235:X:17383125:17391615:-1 gen... [more]
F10D7.55.512e-1325.00pep chromosome:WBcel235:X:17388272:17391561:-1 gen... [more]
F10D7.52.048e-1225.40pep chromosome:WBcel235:X:17385978:17391561:-1 gen... [more]
F10D7.51.250e-825.81pep chromosome:WBcel235:X:17383125:17386535:-1 gen... [more]
K11D12.94.926e-741.67pep chromosome:WBcel235:V:5042408:5046203:-1 gene:... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
neur2.242e-2231.21gene:FBgn0002932 transcript:FBtr0082002[more]
neur2.267e-2231.21gene:FBgn0002932 transcript:FBtr0082001[more]
neur2.267e-2231.21gene:FBgn0002932 transcript:FBtr0300414[more]
neur3.692e-2231.21gene:FBgn0002932 transcript:FBtr0082003[more]
neur3.854e-2231.21gene:FBgn0002932 transcript:FBtr0082004[more]
back to top
BLAST of Neuralized, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
neurl1ab1.139e-2022.33neuralized E3 ubiquitin protein ligase 1Ab [Source... [more]
neurl1ab4.082e-2022.33neuralized E3 ubiquitin protein ligase 1Ab [Source... [more]
neurl1ab4.270e-2022.33neuralized E3 ubiquitin protein ligase 1Ab [Source... [more]
neurl1b2.725e-1931.43neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
neurl1b2.806e-1931.43neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
neurl17.133e-2321.32neuralized E3 ubiquitin protein ligase 1 [Source:N... [more]
kctd104.491e-2032.20potassium channel tetramerization domain containin... [more]
kctd105.090e-2032.20potassium channel tetramerization domain containin... [more]
ENSXETT00000038138.11.687e-1228.77neuralized E3 ubiquitin protein ligase 3 [Source:N... [more]
neurl46.036e-1122.56neuralized E3 ubiquitin protein ligase 4 [Source:N... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Neurl1a3.539e-2126.56neuralized E3 ubiquitin protein ligase 1A [Source:... [more]
Neurl1a3.612e-2126.56neuralized E3 ubiquitin protein ligase 1A [Source:... [more]
Neurl1b9.012e-2132.79neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
Neurl1b1.371e-2029.89neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
Neurl1a7.287e-2029.56neuralized E3 ubiquitin protein ligase 1A [Source:... [more]
back to top
BLAST of Neuralized, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P29503|NEUR_DROME2.235e-2131.21Protein neuralized OS=Drosophila melanogaster OX=7... [more]
sp|Q24746|NEUR_DROVI3.072e-2130.13Protein neuralized OS=Drosophila virilis OX=7244 G... [more]
sp|Q923S6|NEUL1_MOUSE2.475e-2026.56E3 ubiquitin-protein ligase NEURL1 OS=Mus musculus... [more]
sp|A8MQ27|NEU1B_HUMAN2.894e-2029.87E3 ubiquitin-protein ligase NEURL1B OS=Homo sapien... [more]
sp|Q0MW30|NEU1B_MOUSE8.464e-2032.79E3 ubiquitin-protein ligase NEURL1B OS=Mus musculu... [more]
back to top
BLAST of Neuralized, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Y2H5K22.664e-3225.53E3 ubiquitin-protein ligase NEURL1 OS=Araneus vent... [more]
A0A2L2YNI64.592e-3224.89Neuralized-like protein 1A OS=Parasteatoda tepidar... [more]
A0A3S3PAW95.543e-2923.36Protein neuralized-like protein OS=Dinothrombium t... [more]
A0A3S4RI897.280e-2923.36Protein neuralized-like protein OS=Dinothrombium t... [more]
H2YX888.625e-2724.06Uncharacterized protein OS=Ciona savignyi OX=51511... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
neurl1b2.779e-2021.24neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
neurl1b1.626e-1931.74neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
neurl1aa6.447e-1827.32neuralized E3 ubiquitin protein ligase 1Aa [Source... [more]
ENSAMXT00000001187.21.333e-1024.84protein neuralized-like [Source:NCBI gene;Acc:1030... [more]
ENSAMXT00000056033.11.814e-1024.84protein neuralized-like [Source:NCBI gene;Acc:1030... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkey-49c17.37.267e-746.81ring finger protein 157 [Source:HGNC Symbol;Acc:HG... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuralized, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO318302.885e-929.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO434235.956e-945.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447846.091e-824.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO396008.181e-825.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380283.194e-751.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Neuralized, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
neurl1b1.141e-1832.28neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
neurl1b1.265e-1832.28neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
neurl1b1.284e-1832.69neuralized E3 ubiquitin protein ligase 1B [Source:... [more]
ENSORLT00000007585.24.212e-1828.57E3 ubiquitin-protein ligase NEURL1 [Source:NCBI ge... [more]
ENSORLT00000027315.14.212e-1828.57E3 ubiquitin-protein ligase NEURL1 [Source:NCBI ge... [more]
back to top
BLAST of Neuralized, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038756.10.000e+098.71SMESG000038756.1[more]
SMESG000023072.12.378e-734.57SMESG000023072.1[more]
SMESG000023072.12.554e-734.57SMESG000023072.1[more]
SMESG000023072.12.784e-734.57SMESG000023072.1[more]
SMESG000023072.12.820e-734.57SMESG000023072.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019009 ID=SMED30019009|Name=Neuralized, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=1702bp
TTTAATTTAAAATTGCAAATTTTCCAACAATAACAAAATTTATATATTTT
ATTATTTCAAAAATTTTGATTGTAAAATTAAATTATGGGAAATACGCCAA
ATAGCAAGTCTCAACCGGTTCGTCCACCAATGAAAATTCATCAAATTCAT
GGAAAAAATTTAACTGTTGGAGCAGATAGATTATCCGTGAAACGACATGA
AGGATATTCAGATGGTATATTTTTTGGATCAAGACCCGTCTTTCATGGCG
AAAAGATTGAAATCACAGTTCTTAAAATGATAAGTGAATGGAATGGAGCA
TTGAGATTTGGATTCACAACTCATAATCCAATAGCCATTAGTAATCATGC
TGAAGGTATTCCAAGATATATGTGTCCTGACTTAATTAAAAGAGGAGGAT
CTTATGTTGAAACAATTAGCCAAATATCCTTAAGAGTAAAAGATAAAATT
GTATATTGGTTAGAGTCAACTGGTGAAGTGAGTCTCAAAGTAAATGAAGT
GATGCAAAAGTGCCAACTACATGGCATCGTTAAAGATGTATGGCCAGTAG
TTGATGTTTACGGGAATACAGTTGAAGTAACTTTTCGTAGTTTAGATGAT
AACGTCGATTTGATTTACACACCAACGGAACCCAGATTTTATTCATCATT
GAGATTGTCACAATTTACACTATCTGCAAATGCAGTATCTGTGGAACTTG
TGGACTCAGATACTGTCTGTAAAAAAGGTAATATAAAAGCATTTGCTTGG
AATCAAAATCCTTTCCCAACAAATACAATATGGGTATTATTTGTTAATGA
AGTTGATCATAGTATTGGCAATAATTTATCTATTGCTATTTTAAAACTGG
CACCTCAAGTTAATAATTTACCTGAAAAATTGTCTGATTTTGCTCGAGGA
AATGGCTGGATTTTTGAAAATGTATATATAAGAATGGTTCCAGGAGATGA
AGTAGCTATTTGTATTTTGGATTCAGGAGTTGTACAAGTGGCAGTCAACG
GGGAAAGATTTGAAGAATTAGGTAAAGTTTCCCCGAATTCATTTTATTGG
ATAGCAGTCGATATGAACTGCAGTCTGATAAACTTAACCAGCGTTGGGTT
ATATAAGATGACTCGCTATCCATTGCCAATCCTTCCGTCTATTCCGAATA
TATTGGAATTGGATATCAGAGGAGCTGCTTCAGCATTGCCGTCAGGTGAT
TACAAACGGCCCACAACAAAGGAAATCCAGTATATTCCAGTCAGAGAAGA
ATCCAATCGTCAGGAACTACCTAGAAATAATCCAACCAAACCTAGGGAAT
ATGATTTTCGTGCTGAATGTGTAATTTGCTTAGATACAATTCCAGATAAT
GTATTTATTCCCTGTGGTCATGTTTGCTGTTGTAAGGCTTGTGCAGACAA
TCTGATGAATGAGCATGGGAGTATTTTGTGCCCAACTTGCAGGAAAAAAA
TCGAGCACAGCTACAAAGTATATATGTAATGATTTCACCTTGGCTGAATT
TCTATCAAGCTTGTTGGATTAATAATTTTTGTGATGAATAAATTGGTTTA
TTACAATATTTTACTGATTGAAAAATATTTTTACTAAAATATTTTCACCT
TGGCTGAATTTCTATCAAGCTTGTTGGATTAATAATTTTTGTGATGAATA
AATTGGTTTATTACAATATTTTACTGATTGAAAAATATTTTTACTAAAAT
AT
back to top

protein sequence of SMED30019009-orf-1

>SMED30019009-orf-1 ID=SMED30019009-orf-1|Name=SMED30019009-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=465bp
MGNTPNSKSQPVRPPMKIHQIHGKNLTVGADRLSVKRHEGYSDGIFFGSR
PVFHGEKIEITVLKMISEWNGALRFGFTTHNPIAISNHAEGIPRYMCPDL
IKRGGSYVETISQISLRVKDKIVYWLESTGEVSLKVNEVMQKCQLHGIVK
DVWPVVDVYGNTVEVTFRSLDDNVDLIYTPTEPRFYSSLRLSQFTLSANA
VSVELVDSDTVCKKGNIKAFAWNQNPFPTNTIWVLFVNEVDHSIGNNLSI
AILKLAPQVNNLPEKLSDFARGNGWIFENVYIRMVPGDEVAICILDSGVV
QVAVNGERFEELGKVSPNSFYWIAVDMNCSLINLTSVGLYKMTRYPLPIL
PSIPNILELDIRGAASALPSGDYKRPTTKEIQYIPVREESNRQELPRNNP
TKPREYDFRAECVICLDTIPDNVFIPCGHVCCCKACADNLMNEHGSILCP
TCRKKIEHSYKVYM*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR043136B30.2/SPRY_sf
IPR037962Neuralized
IPR006573NHR_dom
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006573Neuralized homology repeat (NHR) domainSMARTSM0058827bcoord: 13..137
e-value: 4.2E-17
score: 72.8
IPR006573Neuralized homology repeat (NHR) domainPFAMPF07177Neuralizedcoord: 216..335
e-value: 1.6E-5
score: 25.0
coord: 19..167
e-value: 7.4E-30
score: 103.9
IPR006573Neuralized homology repeat (NHR) domainPROSITEPS51065NHRcoord: 15..170
score: 32.76
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 396..464
e-value: 4.8E-16
score: 59.8
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 411..458
e-value: 2.9E-12
score: 46.3
NoneNo IPR availablePANTHERPTHR12429:SF6PROTEIN NEURALIZEDcoord: 1..463
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 411..458
IPR043136B30.2/SPRY domain superfamilyGENE3DG3DSA:2.60.120.920coord: 17..169
e-value: 5.7E-34
score: 119.2
IPR037962NeuralizedPANTHERPTHR12429NEURALIZEDcoord: 1..463
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 412..453
score: 11.84