SMED30018977

Overview
NameSMED30018977
Smed IDSMED30018977
Length (bp)755
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018977 (SMED30018977) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018977 (SMED30018977) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018977 (SMED30018977) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018977 (SMED30018977) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018977

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30018977SMESG000058604.1 Contig3655newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018977SMESG000058604.1 Contig3655uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018977SMESG000036432.1 Contig3655newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018977SMESG000036432.1 Contig3655uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30018977 dd_Smed_v4_15804_0_3dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30018977 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.239e-25
Identity = 51/143 (35.66%), Postives = 77/143 (53.85%), Query Frame = -1
Query:  171 MKKRKGDTHYNADALLRTGCYTWIQYQTIHEEPXXXXXXXXXXXLICQEDGKL*QQHSKEIENVKRYTNMAQTNFIESNDIIQSGKGKIWIPEERKIEVVLYTHKILCHAGVTKVHEYFSRKFDMRNIQEFNKEVFGSYENCQ 599
            M+ RKG +H NAD L R  C T +Q    HE+ KTGK+KT++L +  +      Q  + E++ +K         FI  N+ + + +GKIWIP + +  ++   H +LCHAG  KV +Y     DM N+    K+V  + E CQ
Sbjct: 3129 MEYRKGTSHTNADMLSRKTCGTCVQCMMEHEDAKTGKIKTRILTVTAEGGYNKWQNDNMEVQEIKNKLENKDCKFIMENNTVLTKQGKIWIPSDNRQRMIKEVHVLLCHAGAQKVTKYIQNNCDMENLATEVKKVIENCERCQ 3271          

HSP 2 Score: 59.3066 bits (142), Expect = 5.239e-25
Identity = 27/51 (52.94%), Postives = 35/51 (68.63%), Query Frame = -2
Query:  599 YFTEHFKHYLYSKMFQ*RTNHKAITFMLMINRAITPRF*TWINQFTGLNFE 751
            YF +HF HYLY K F  RT+HKAITFM+   + IT +F TWIN  + L+ +
Sbjct: 3078 YFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTWINYLSSLDIK 3128          
BLAST of SMED30018977 vs. Planmine SMEST
Match: SMESG000007341.1 (SMESG000007341.1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.780e-17
Identity = 44/79 (55.70%), Postives = 55/79 (69.62%), Query Frame = -1
Query:   81 RKIEVVLYTHKILCHAGVTKVHEYFSRKFDMRNIQEFNKEVFGSYENCQKRKAMTTQQQKNKLNLKEFIYLNKFSSTFV 317
            ++ E VLYTH+I+C AG   VHEY SRKF MRN QEF KEV  S ENCQKRKAM+T+  + ++  K  +YL  F S F+
Sbjct:    6 KETEFVLYTHRIMCRAGGNNVHEYISRKFYMRNRQEFIKEVVRSCENCQKRKAMSTRTAEKQIKPKG-VYL--FESIFI 81          

HSP 2 Score: 47.7506 bits (112), Expect = 3.074e-6
Identity = 23/27 (85.19%), Postives = 24/27 (88.89%), Query Frame = -3
Query:   67 TAEEQIKPKGVYLFE*IFIDFCGSMKT 147
            TAE+QIKPKGVYLFE IFIDF G MKT
Sbjct:   63 TAEKQIKPKGVYLFESIFIDFYGPMKT 89          
BLAST of SMED30018977 vs. Planmine SMEST
Match: SMESG000053301.1 (SMESG000053301.1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.662e-13
Identity = 37/99 (37.37%), Postives = 55/99 (55.56%), Query Frame = -1
Query:  141 QQHSKEIENVKRYTNMAQTNFIESNDIIQSGKGKIWIPEERKIEVVLYTHKILCHAGVTKVHEYFSRKFDMRNIQEFNKEVFGSYENCQKRKAMTTQQQ 437
            Q+ S EI+ +K Y++   + F   N ++++ +GKIWI +E +   +   HK LCHAG  KV +Y    F M N+    KEV    EN QKRK  T + +
Sbjct:   10 QKDSNEIKTIK-YSDSTNSKFKVENKLVKTIEGKIWITDESRNAFIGEYHKKLCHAGSNKVKKYICHNFVMNNLSSKEKEVIERCENSQKRKTYTNRTK 107          
BLAST of SMED30018977 vs. Planmine SMEST
Match: SMESG000049233.1 (SMESG000049233.1)

HSP 1 Score: 49.6766 bits (117), Expect = 4.620e-13
Identity = 21/50 (42.00%), Postives = 33/50 (66.00%), Query Frame = -2
Query:  599 FTEHFKHYLYSKMFQ*RTNHKAITFMLMINRAITPRF*TWINQFTGLNFE 748
            +  +FK YLY K+F  RT+HKA+ F+    +AI+P+F TW+   +  +FE
Sbjct:  187 YVNYFKQYLYGKLFMVRTDHKALIFLNTTKKAISPQFQTWMVNLSDYDFE 236          

HSP 2 Score: 42.3578 bits (98), Expect = 4.620e-13
Identity = 54/198 (27.27%), Postives = 88/198 (44.44%), Query Frame = -1
Query:   81 F*MKKRKGDTHYNADALLRTGCYTWIQYQTIHEEPXXXXXXXXXXXLICQEDGK----L*QQHSKEIENVKRYTNMAQTNF---------------IESND----IIQSGKGKIWIPEERKIEVVLYTHKILCHAGVTKVHEYFSRKFDMRNIQEFNKEVFGSYENCQKRKAMTTQQQKNKLNLKEFIYLNKFSSTFV 605
            F +K  KG+ H NAD L R G     Q QT HE  K  K++ K + +I + +        Q+ +K+ E +K+  N    NF               ++ ND    + ++G   + +P E   ++V   H+ +C  GV K+  Y +  F   N+Q   KE     + C +RK    Q+Q  ++ L   I  ++FS   V
Sbjct:  235 FELKYHKGEDHSNADGLSRIGGMLCSQCQTRHENAKEAKVRVKHICMIQKTEVMHQLFEAQESNKQFEKMKKCVN--NGNFGDFDKKDFFFKLRDNLKMNDGLLIVNKNGMDLLLVPIEFTQQLVTKIHEKMCRVGVKKLVHYINNIFYWPNLQSTIKECLQKCQECARRK--VNQEQTKEILLPHVI--SEFSDQIV 426          
BLAST of SMED30018977 vs. Planmine SMEST
Match: SMESG000049233.1 (SMESG000049233.1)

HSP 1 Score: 49.6766 bits (117), Expect = 5.005e-13
Identity = 21/50 (42.00%), Postives = 33/50 (66.00%), Query Frame = -2
Query:  599 FTEHFKHYLYSKMFQ*RTNHKAITFMLMINRAITPRF*TWINQFTGLNFE 748
            +  +FK YLY K+F  RT+HKA+ F+    +AI+P+F TW+   +  +FE
Sbjct:  215 YVNYFKQYLYGKLFMVRTDHKALIFLNTTKKAISPQFQTWMVNLSDYDFE 264          

HSP 2 Score: 42.3578 bits (98), Expect = 5.005e-13
Identity = 54/198 (27.27%), Postives = 88/198 (44.44%), Query Frame = -1
Query:   81 F*MKKRKGDTHYNADALLRTGCYTWIQYQTIHEEPXXXXXXXXXXXLICQEDGK----L*QQHSKEIENVKRYTNMAQTNF---------------IESND----IIQSGKGKIWIPEERKIEVVLYTHKILCHAGVTKVHEYFSRKFDMRNIQEFNKEVFGSYENCQKRKAMTTQQQKNKLNLKEFIYLNKFSSTFV 605
            F +K  KG+ H NAD L R G     Q QT HE  K  K++ K + +I + +        Q+ +K+ E +K+  N    NF               ++ ND    + ++G   + +P E   ++V   H+ +C  GV K+  Y +  F   N+Q   KE     + C +RK    Q+Q  ++ L   I  ++FS   V
Sbjct:  263 FELKYHKGEDHSNADGLSRIGGMLCSQCQTRHENAKEAKVRVKHICMIQKTEVMHQLFEAQESNKQFEKMKKCVN--NGNFGDFDKKDFFFKLRDNLKMNDGLLIVNKNGMDLLLVPIEFTQQLVTKIHEKMCRVGVKKLVHYINNIFYWPNLQSTIKECLQKCQECARRK--VNQEQTKEILLPHVI--SEFSDQIV 454          
The following BLAST results are available for this feature:
BLAST of SMED30018977 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018977 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029259.15.239e-2535.66SMESG000029259.1[more]
SMESG000007341.11.780e-1755.70SMESG000007341.1[more]
SMESG000053301.13.662e-1337.37SMESG000053301.1[more]
SMESG000049233.14.620e-1342.00SMESG000049233.1[more]
SMESG000049233.15.005e-1342.00SMESG000049233.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018977 ID=SMED30018977|Name=SMED30018977|organism=Schmidtea mediterranea sexual|type=transcript|length=755bp
CATCTTGACCAGAAGTTGCACTCAAAGAAATATATTTACCAAAATATATC
TCTTTGCATTCCTAGAAGTTTTCATTGAGCCACAAAAGTCGATGAAAATT
TATTCAAATAAATAAACTCCTTTAGGTTTAATTTGTTCTTCTGCTGTTGG
GTAGTCATGGCTTTTCTTTTTTGACAATTTTCGTACGATCCGAATACTTC
TTTATTAAATTCTTGTATGTTTCTCATATCGAATTTCCTAGAAAAATATT
CGTGAACTTTAGTCACTCCAGCATGACACAAAATTTTATGAGTGTACAAA
ACAACTTCTATTTTTCTTTCTTCTGGAATCCATATTTTACCTTTACCTGA
CTGAATAATGTCATTACTTTCTATAAAATTTGTTTGTGCCATATTAGTAT
ATCTCTTGACATTTTCTATTTCTTTAGAATGTTGTTGCTACAGTTTTCCA
TCTTCCTGGCATATGAGTACTAGAACTTTTGTTTTTAGTTTTCCTGTTTT
TGGTTCCTCGTGAATCGTTTGATATTGTATCCACGTATAACATCCAGTTC
TTAACAGTGCATCTGCATTATAATGCGTGTCACCCTTACGTTTTTTCATT
CAAAATTTAGACCAGTAAATTGGTTGATCCATGTTTAAAATCGAGGTGTG
ATTGCTCTGTTGATCATCAACATAAACGTTATTGCTTTATGATTTGTTCT
CTATTGAAACATTTTGCTATATAAATAGTGCTTGAAATGTTCGGTAAAAT
AAATT
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protein sequence of SMED30018977-orf-1

>SMED30018977-orf-1 ID=SMED30018977-orf-1|Name=SMED30018977-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
MAQTNFIESNDIIQSGKGKIWIPEERKIEVVLYTHKILCHAGVTKVHEYF
SRKFDMRNIQEFNKEVFGSYENCQKRKAMTTQQQKNKLNLKEFIYLNKFS
STFVAQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte