BED-type domain-containing protein

Overview
NameBED-type domain-containing protein
Smed IDSMED30018950
Length (bp)2209
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of BED-type domain-containing protein (SMED30018950) t-SNE clustered cells

Violin plots show distribution of expression levels for BED-type domain-containing protein (SMED30018950) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of BED-type domain-containing protein (SMED30018950) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for BED-type domain-containing protein (SMED30018950) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018950

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30018950 dd_Smed_v4_24095_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of BED-type domain-containing protein vs. TrEMBL
Match: B0W9I7 (Predicted protein OS=Culex quinquefasciatus OX=7176 GN=6035136 PE=4 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 3.795e-53
Identity = 135/421 (32.07%), Postives = 209/421 (49.64%), Query Frame = 2 
Query:  545 AKCTIC-KNEKDLVANTSTLARHLTSIHNINIKSAHLDSTIIATQRV-------------QTNVISFLQRKTLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKE----RLLDINQAIETEVLEDNFNEDEYPMEGDEDEEFYLNEDIESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRK 1753
            A+C  C K       +TS L RH  ++HN  +K   +  +++  + V             Q+ +  F+ +K +  + A LAA DG +I  ITNSKFIR S   +GY LP  +T VM L+H +Y+  K+  +QQI D  +E    S+TLDE   +  RK+ NV ++       NLGLVR+ GS  AEK+ EAV+ HL  F I   R++ AAT D A  M KFG+L+   +Q+C NH  HL V    +   E     L + +   E    E++ +  +  + G      Y + +   +L  +R I  LFRRS +K  +L KK+ +                  +  + ++L LDVK RWN+   M+  ++K +  + +AL EL++   +   + E++  L + L P ELA    GK +    K
Sbjct:   16 AQCKKCPKTISCKGGSTSGLLRHGVNVHNFRVKR-RIPESVVQPEDVPSGSKQAKSNTSKQSTIEPFIHKKPMNEMFARLAAQDGLTISAITNSKFIRTSFAQRGYQLPLSKTSVMKLIHDYYEIAKQQTVQQIQDKLAELHFLSITLDEWTSSGNRKYLNVNVHFGDGAFFNLGLVRVKGSFPAEKILEAVDQHLECFAIR-DRNVAAATSDAAKVMEKFGKLATFKHQLCFNHGLHLAVCDVIFKSSETPGDLLEESDSDDEDCDSEESDDSKDATIPG-----LYNSSNYRPVLLQIRNICKLFRRSPVKNAILQKKVVQ------------------EFGKPLQLILDVKTRWNSTEQMLARYMKLHGCVAEALKELEDLAPVSTSDLESISQLYDALQPDELAAFINGKAEGPSNK 411
[Close]
BLAST of BED-type domain-containing protein vs. TrEMBL
Match: A0A0N7Z9Z3 (BED-type domain-containing protein OS=Scylla olivacea OX=85551 PE=4 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.617e-50
Identity = 139/429 (32.40%), Postives = 213/429 (49.65%), Query Frame = 2 
Query:  461 DNIMASNEKESWVWKYFIKRIVNRNTVGAKCTI---CKNEKDLVANTSTLARHLTSIHNI----NIK-----SAHLDSTIIATQRVQTNVISFLQRK-TLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKE-RLLDINQAIETEVLEDNFNEDE-YPME----------GDEDEEFYLNEDIES----------MLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEE 1642
            D   A+   ES+ WKYF K     ++   KC     C+       +TS + RHL S H+I    N+K     S   D++ +    VQ  +   L+++ T E ++ +LAAVDGFSI  I  S+FI+ S+ A+GY LP+  T VM LVHK Y+  KE  I  I   K+  ++F ++LDE      +++ N+ L+ S +   NLG+ RI GS  AE   E V N L  F ++  RD+VA   DGAS M+KFG+L    +  C  H  HL V    Y K +  L+ + +   T + E+  ++ E  P E           DED E    ED+E            +  VR+IV +FR+S  + + L     ++ VV+E               +++ L+LD K RWN+++ M+  F+K    +  A+I+ + E
Sbjct:    3 DEAEATTSTESYAWKYFEKLKCEESSRCKKCRAVIKCRG-----WSTSGMIRHLKSKHSIEKTENLKRPSDRSHDKDTSEVKRNSVQQKMTDLLKKEETKEEIVGKLAAVDGFSINAIAKSEFIKTSMLARGYKLPQSPTLVMDLVHKQYNVAKERVIFDINKRKNVGVRFGLSLDEYTSLKNKRYMNINLHTS-DTFWNLGMARITGSVPAETAVEVVENKLAEFQVNLERDVVACVTDGASVMVKFGKLIKTCHHTCYAHGIHLAVCDVLYKKTDGNLVSLERRNVTHLQEEEADDVEAVPEEEELSSMIEVVSDEDLENIRLEDLEDDEVGNIDLSVTINKVRKIVKIFRKSPTRNEKL-----QMYVVSE-------------YGKELMLKLDSKTRWNSLLDMLERFLKVKNSVAKAMIDCNME 407
[Close]
BLAST of BED-type domain-containing protein vs. TrEMBL
Match: A0A226D4I7 (Putative AC transposase OS=Folsomia candida OX=158441 GN=Fcan01_25450 PE=4 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 4.885e-47
Identity = 144/475 (30.32%), Postives = 226/475 (47.58%), Query Frame = 2 
Query:  497 VWKYFIKRIVNRNTVGAKC--TIC-KNEKDLVANTSTLARHLTSIHNINIKSAHLDSTIIATQRVQTNVIS-FLQRKT--LEYLIAELAAVDGFSIRGITNSKFIRESITAKGY-HLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLEDNFNEDEYP---------------MEGDEDEEFYLNEDIESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRKAMVSIEFVRDKISQNTSAISLKLLQNINEYIDDR 1855
            +W YF+K + +  T  AKC  T C K  K    +TS L  HL SIH+I++    L+ + +  +      IS F ++KT  LE  +A + A DG      + S  +R ++ + G+  LP+    +  +V  +  ++K    + ++D K    KFSVT DE      R++ NV L+       NLGL+RI GS  AEK  E V N L    +    DIV    DG+S M K GRLSP   Q+C  H   LGV+   Y K      INQ  E +  E+   ++  P               ++ DEDE+  ++  +  ++  VR IV +FRRS LK ++L   I                  K++  +++ L LD K RWN++V M+  F      IK AL+++  +  L       +  +++ L PIE  +  L +RDA L  A  ++ F+  K+    S ++L+L   +   I++R
Sbjct:   35 IWNYFLKEVADGKT--AKCMDTSCQKVLKTAGGSTSGLNTHLRSIHHIDLLKKDLNPSELGVKAKSVKPISDFFEKKTKSLEETLARMTAKDGIPFSVFSTSTDLRRALKSDGFTDLPKSAHTIKKIVIGYSQKIKSTENEHVLDLKRIGRKFSVTFDEWSSIKNRRYMNVILHGKPQEFWNLGLIRIHGSLPAEKCLEMVANKLRLLNLSLENDIVCIITDGSSVMTKIGRLSPTYQQLCFAHGIQLGVLDVLYKK------INQTPEADESENENLDNLAPCEDDSCCDSDSDSEEIDEDEDEDLVISHTLRPVIDKVRTIVRMFRRSPLKNEILQTYI------------------KQEFGKELSLILDCKTRWNSLVAMLERFYTVQASIKKALVDVRSDAHLKDWELARISDIVDALKPIEATIEALCRRDANLLTADTALNFMIQKLDMQQSGLALELGVALRTRIEER 483
[Close]
BLAST of BED-type domain-containing protein vs. TrEMBL
Match: A0A4C1Y486 (BED-type domain-containing protein OS=Eumeta variegata OX=151549 GN=EVAR_52339_1 PE=4 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 1.049e-45
Identity = 119/347 (34.29%), Postives = 183/347 (52.74%), Query Frame = 2 
Query:  497 VWKYFIKRIVNRNTVGAKCTICKNE-KDLVANTSTLARHLTSIHNINIKSAHLDSTIIAT--------QRVQTNVISFLQRKTLEY----------LIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKS-ENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLEDNFNEDEYPMEGDED-----EEFYLN--EDIESMLASVRRIVVLFRRSSLKTDLLHKKIQ 1456
            VWKYF K         A+C  C  +     +NT+ L RHL  IH I+IK+    ++  A         Q   +       +KTL +          ++A LAA DG +IR IT S FIRES+  +G +LP+ E+ VM ++ K+Y++ K+  I  + + K  E+ +F++++DE      R++FN+ ++ + +   NLGLV I G C A ++ E + + L  FG+ F +D+VA T DG + M+KFGR +P    +CLNHA HL V++TF  K E     N+   +    DN + +E   E DED      E   N  ++I+  L   R +V LFR+S LK   L K ++
Sbjct:    6 VWKYFTK---TPKEGLAQCLKCPKKISTKGSNTTGLVRHLEHIHKISIKNTETQASTSAGYSADNRDEQPSDSKKSKSKLQKTLHFEPKNKQSLGEIVAVLAAKDGLTIRQITKSDFIRESLRLRGINLPKNESDVMKIILKYYEEKKQETIDFLSNSKQKESARFNLSVDEWSSLRNRRYFNICIHHTDSNFYNLGLVYIPGQCRAVEIKEIIKHRLEEFGLYFEQDVVAVTSDGPNVMVKFGRENPTEMVLCLNHAVHLAVLETFCKKNE----TNENFTSNQDSDNLSGEE--SEYDEDNCDLEPEIRTNYTQNIDKALQDTRALVKLFRKSPLKNITLQKHVK 343
[Close]
BLAST of BED-type domain-containing protein vs. TrEMBL
Match: A0A226DDR7 (Putative AC9 transposase OS=Folsomia candida OX=158441 GN=Fcan01_16136 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 1.117e-41
Identity = 168/635 (26.46%), Postives = 280/635 (44.09%), Query Frame = 2 
Query:  497 VWKYFIKRIVNRNTVGAKCTICKNEKDLV----ANTSTLARHLTSIHN-----INIKSAHLDSTIIATQRVQTN-----VISFLQRK--TLEYLIAELAAVDGFSIRGITNSKFIRESITAKGY-HLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGR-LSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLEDN--FNED------EYPMEGDEDEE--FYLNED---------IESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINI---LTPIELAVLELGKRDATLRKAMVSIEFVRDKISQNTSAISLKLLQNIN--------------EYIDDRMDPNLFHLIECLDTAAVPPNSVLKFATEIMKR-----LFGTDETNDENESDNILATVSREDNNFSSLAQELTQRINSSSIPKTL------QLKFDKLKQEFQIFKSTGNRTENLERLRNALDTIQPTS 2206
            +W YF+K         AKC   + +K ++     +T     HL  IHN     IN+ S  LDS     QR   +     +  +L  K  TL+   A +AA+DG S + + NS  IR  + ++G+  +      +   V KF   +++   ++I+    +  KFS+T DE      R++ +V L   +    NLGLV I+GS TAE   + V+  + S G++  +DIVA   DG+S M K GR  +P   Q+CL H   L V+   Y K E + D +     E +++N  F++D      EY    DE+ E  F L E+         ++ ++  +R  V +FRRS  K ++L   +                  K +  +++ L LD + RWN++  MI  F      IK A I+L   R+     ++    +  +   L PI  AV  +  R++ L     ++  V  K+    S++SL+L  +I                Y+ +R+  N     E  DT  +    V  F  +++ R     + G +E+ND   +++     S      ++   EL + +  +  P T       Q     L+QE ++F+  G R  NL  + + L T++PTS
Sbjct:   22 IWHYFLKESTG---ATAKCKDPECKKPILKTSGGSTKGCRDHLKRIHNVDLDIINLTSVDLDS---GRQREHASKKSKRITDYLSVKHDTLQIAYARMAALDGLSFQVMANSYDIRAGLKSRGFSEVLSSPNAIKSKVRKFAQSLRDNDRKEIMQLNEQGTKFSITFDEWTSMRNRRYLSVILLGKQQQFWNLGLVPILGSATAENCLQLVDEKITSVGLNLHKDIVAIITDGSSVMKKIGRDAAPTFQQLCLAHGIQLAVLDVLYAKGENVGDTDN---LEAIDENSSFSDDEGSEFVEYASPDDENAETDFDLTEESDAPSLNPILKPIIDKIRSTVKMFRRSPKKNEILQNYV------------------KSEFQKELNLILDCRTRWNSLAAMIERFHLIRTCIKKAFIDL---RVATNFEEQEFNYIFQVMEALKPIRAAVEVICNRESNLITTDAALNLVISKLRAQKSSVSLELASSIKFRVMERRTELTKVLRYLHNRVYANEDE--ELADT--ITRTEVQNFLKKMLTRTRIGTVVGDEESNDTPANEDFTVQPSAA----ATFKDELNEIMKKAMTPPTTEKSKKSQTLLTTLRQEMKLFEDGGTRGYNLSLIYDYLLTVKPTS 618
[Close]
BLAST of BED-type domain-containing protein vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1)

HSP 1 Score: 218.779 bits (556), Expect = 3.869e-63
Identity = 154/488 (31.56%), Postives = 253/488 (51.84%), Query Frame = 2 
Query:  479 NEKESWVWKYFIKRIVNRNTVGAKCTICKN-EKDLVANTSTLARHLTSIHNINIKSAHLDSTIIATQRVQTNVISFLQRKTLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLED--NFNE----DEYPMEGDEDEEFYL----NEDIESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRKAMVSIEFVRDKISQNTSAISLKLLQNINEYIDDRMDPNLFHLIECLDTAAVP 1909
            N K+S VWK F K   N     AKCT+C +  K    NTS LA HL + H+I++K         A  +  +++++F++R+++E L+A+ AA DGFSI  +T+S  IRE IT + Y +PR E+ +  L+  F ++ K     +        + FS+T DE    + +++ NVTL+ S+     LGL+ I  SC A ++   V   L+ +GI   R+I+ +T DGAS M K+ +L+ +  Q C+NHA HL V++TFY+K   + +I+ +   +  ED   FN         ++ D D   Y     + +I  +L + R+ V  FR+S ++ D+L + +++                  +  +K+KL LD + RWN+I+ M+   +     IK AL E+  + +    +   L  LI IL P ELAV EL K  +TL  A   + F+  ++ QN +A+  K   ++   I +R +  L  L + L +   P
Sbjct:    2 NSKKSEVWKIFQKETDN----TAKCTMCNSILKCASGNTSNLANHLRAKHDIDVKKNEEPPEKRA--KATSSMLNFIKRESVEELLAKCAAYDGFSIDAMTHSSAIREFITTRSYVMPRSESTIKKLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCSGKRYLNVTLH-SQEDSYKLGLIAIECSCDAYQIHSLVEQKLMDYGIKL-RNILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGYHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVED------------------KEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGTFP 463
[Close]
BLAST of BED-type domain-containing protein vs. Planmine SMEST
Match: SMESG000026901.1 (SMESG000026901.1)

HSP 1 Score: 218.779 bits (556), Expect = 1.548e-62
Identity = 163/516 (31.59%), Postives = 264/516 (51.16%), Query Frame = 2 
Query:  479 NEKESWVWKYFIKRIVNRNTVGAKCTICKNE-KDLVANTSTLARHLTSIHNINIKSAHLDSTIIATQRVQTNVISFLQRKTLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLED--NFNE--DEYPMEGDED----EEFYLNED--IESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRKAMVSIEFVRDKISQNTSAISLKLLQNINEYIDDRMDPNLFHLIECLDTAAVP-------PNS----VLKFATEIMKRLFG 1960
            N K+S VWK F K   N     AKCT+C +  K    NTS LA HL + H+I++K         A  +  +N+++F++R+++  L+A+ AA DGFSI  +T+S  IRE IT + Y +PR E+ +  L+  F ++ K     +        +  S+T DE    + +++ NVTL+ S+     LGL+ I GSC A ++   V   L+ +GI   R+I+A+T DGAS M K+ +L+ +  Q C+NHA HL V++TF++K   + +I+ +   +  ED   FN    ++ +  D D    E  Y  +D  I  +L + R+ V  FR+S ++ D+L + +++                  +  +K+KL LD + RWN+++ M+   +     IK AL E+  + +    +   L  LI IL P ELAV EL K  +TL  A   + F+  ++ QN +A+      ++   I +R +  L  L + L +   P       P S     L  AT +  RLFG
Sbjct:    2 NSKKSEVWKIFQKETDNT----AKCTMCNSVLKCAGGNTSNLANHLRAKHDIDVKKNEEPPEKRA--KATSNMLNFIKRESVGELLAKCAAYDGFSIHAMTHSSAIREFITTRSYVMPRSESTIKKLIVDFANEKKIQFKDKFTKMAESGICLSITTDEWTSCSGKRYLNVTLH-SQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKL-RNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFHNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDAKINEILQNFRKTVRFFRKSPVRNDILQRYVED------------------KEGKKLKLILDSRTRWNSLIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKIFYNSLKIRISERRNKELITLYKFLQSGTFPQPDLMELPYSTKAPTLSLATMLAARLFG 491
[Close]
BLAST of BED-type domain-containing protein vs. Planmine SMEST
Match: SMESG000061313.1 (SMESG000061313.1)

HSP 1 Score: 213.386 bits (542), Expect = 4.955e-60
Identity = 185/607 (30.48%), Postives = 294/607 (48.43%), Query Frame = 2 
Query:  479 NEKESWVWKYFIKRIVNRNTVGAKCTICKNE-KDLVANTSTLARHLTSIHNINIKSAHLDSTIIATQRVQTNVISFLQRKTLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLED--NFN----EDEYPMEGDEDEEFYL----NEDIESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRKAMVSIEFVRDKISQNTSAISLKLLQNINEYIDDRMDPNLFHLIECLDTAAVP-------PNS----VLKFATEIMKRLFGTDETNDENESDNILATVSRE-DNNFSSLAQELTQRINS-------SSIPKTLQLKFDKLKQEFQIFKSTGNRTENLERLRNALDTIQPTST 2209
            N K+S VWK F K   N     AKCT+C +  K    NTS LA HL + H+I +K         A  +  +N+++F++R ++  L+A+ AA DGFSI  +T+S  IRE IT   Y +PR E+ +  L+  F             ++KS NL+ S+          +K+ N+           LGL+ I GSC A ++   V   L+ +GI   R+I+A+T DGAS M K+ +L+ +  Q C+NHA HL V++TFY+K   + +I+ +   +  ED   FN    +    ++ D D   Y     + +I  +L + R+ V  FR+S ++ D+L + +++                  +  +K+KL LD + RWN+++ M+         IK AL E+  + +    +   L  LI IL P ELAV EL K  +TL  A   + F+  ++ QN +A+  K   ++   I +R +  L  L + L + A P       P S     L  +T +  RLFG  E N     +NI  +      +  ++L Q L Q I++              +L F K   E +IF  T  +++ ++ L N L +IQPTST
Sbjct:    2 NSKKSEVWKIFQKETDNT----AKCTMCNSVLKCAGGNTSNLANHLRAKHDIYLKKNEEPPEKRA--KATSNMLNFIKRVSVGELLAKCAAYDGFSIHAMTHSSAIREFITTGSYVMPRSESTIKKLIVDF------------ANEKSYNLRTSL----------QKWVNL------EDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKL-RNILASTQDGASVMKKYAQLNKITNQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTKYTYSLDDANINQILQNFRKTVRFFRKSPVRNDILQRYVED------------------KEGKKLKLILDCRTRWNSLIPMMERLFLLQVCIKHALEEIRRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRNKELITLYKFLQSGAFPQPDLMELPYSTKAPTLSLSTMLAARLFG--EPNPPFAEENIEESEVEPIASGSATLVQRLQQSISAVLHDSKECGNDGNFELSFQK---ELKIFGVTKQKSKTVQNLFNVLASIQPTST 550
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BLAST of BED-type domain-containing protein vs. Planmine SMEST
Match: SMESG000022344.1 (SMESG000022344.1)

HSP 1 Score: 213.386 bits (542), Expect = 5.085e-60
Identity = 171/571 (29.95%), Postives = 287/571 (50.26%), Query Frame = 2 
Query:  584 ANTSTLARHLTSIHNINIKSAHLDSTIIATQRVQTNVISFLQRKTLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLED--NFN----EDEYPMEGDEDEEFYL----NEDIESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRKAMVSIEFVRDKISQNTSAISLKLLQNINEYIDDRMDPNLFHLIECLDTAAVPPNSVLK-----------FATEIMKRLFGTDETNDENESDNILATVSRE-DNNFSSLAQELTQRINS-------SSIPKTLQLKFDKLKQEFQIFKSTGNRTENLERLRNALDTIQPTST 2209
             N S LA HL + H+I++K         A  +  +N+++F++R+++  L+A+ A  DGFSI  +T+S  IRE IT + Y +P+ E+ +  L+  F ++ K     + I   +  + FS+T DE    + +++ NVTL+ S+     LGL+ I GSC A ++   V   L+ + I   R+I+A+T DGAS M K+ +L+ +  Q C+NHA HL V++TFY+K   + +I+ +   +  ED   FN    +    ++ D D   Y     + +I  +L + R+ V  FR+S ++ D+L +               ED+  K     K KL LD + RWN+++ M+   +     IK AL E+  + +    +   L+ LI IL P E AV EL K  +TL  A   + F+  ++ QN +A+  K   ++   I +R +  L  L + L +   P   ++K            AT +  RLFG  E N +   +NI  +      +  ++L Q L Q I++        +     +L F K   E ++F  T  +++ ++ L NAL +IQPTST
Sbjct:   11 GNISNLANHLRAKHDIDVKKNEEPPEKRA--KATSNMLNFIKRESVGELLAKCAVYDGFSIHAMTHSSAIREFITTRSYVMPKSESTIKKLIVDFANEKKLQFKDKFIKMANSGMCFSITTDEWTSCSGKRYLNVTLH-SQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMYYDIKL-RNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQR-------------YDEDKEGK-----KFKLILDCRTRWNSLIPMMERLVLLQVCIKHALEEIGCDDLYNEKDFNILENLIEILKPTEFAVKELSKHTSTLITAERVLTFLFTQMKQNNTALGKKFYNSLKIGISERRNKELITLYKFLQSGTFPQPDLMKLPYSTKAPTLSLATMLAARLFG--EPNPQFAEENIEESEVEPIASGSATLVQRLQQSISAVLQDSKECANDGNFELSFQK---ELKMFGVTKQKSKTVQNLFNALASIQPTST 554
[Close]
BLAST of BED-type domain-containing protein vs. Planmine SMEST
Match: SMESG000065760.1 (SMESG000065760.1)

HSP 1 Score: 207.223 bits (526), Expect = 2.891e-58
Identity = 164/508 (32.28%), Postives = 257/508 (50.59%), Query Frame = 2 
Query:  479 NEKESWVWKYFIKRIVNRNTVGAKCTICKNE-KDLVANTSTLARHLTSIHNINIKSAHLDSTIIATQRVQTNVISFLQRKTLEYLIAELAAVDGFSIRGITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKSENLKFSVTLDE*VDATARKFFNVTLNRSRNGVINLGLVRIIGSCTAEKLFEAVNNHLLSFGIDFTRDIVAATGDGASTMLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLED--NFN----EDEYPMEGDEDEEFYL----NEDIESMLASVRRIVVLFRRSSLKTDLLHKKIQELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYYEPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRKAMVSIEFVRDKISQ--NTSAISL-KLLQNINEYIDDRMDPNLFHLIECLDTAAVPPNSVLKFATEIMKRLFG 1960
            N K+S VWK F K   N     AKCT+C +  K    NTS LA HL + H+I++K         A  +  +N+++F++R+++  L+A+ +A DGFSI  +T+S  IRE IT + Y +PR E+ +  L+  F ++ K     +        + FS+T DE    + +++ NVTL+ S+     LGL+ I GSC A ++   V   L+ +GI   R+I+A+T DGAS M K+ +L+ +  Q C+NHA HL V++TFY+K   + +I+ +   +  ED   FN    +    ++ D D   Y     + +I  +L + R+     R+S ++ D+L + +             ED+  K     K+KL LD + RWN+I+ M+   +     IK  L E+  + +    +  NL  LI IL P ELAV EL K  +TL         +R +IS+  N   I+L K LQ+      D M+         L  +   P   L  AT +  RLFG
Sbjct:    2 NSKKSEVWKIFQKETDNT----AKCTMCNSVLKCAGGNTSNLANHLRAKHDIDVKKNEEPPETRA--KATSNMLNFIKRESVGELLAKCSAYDGFSIHAMTHSSVIREFITTRSYVMPRSESTIKKLIVDFANEKKLQFKDKFTKMAKSGMCFSITTDEWTSCSGKRYLNVTLH-SQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKL-RNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTDRFLRKSPVRNDILQRYV-------------EDKEGK-----KLKLILDCRTRWNSIIPMMERLVLLQVCIKHVLEEIGCDDLYNEKDFTNLADLIEILKPTELAVKELSKHTSTL---------LRKEISERRNKELITLYKFLQSGTFPQPDLME---------LPYSTKAP--TLSLATMLAARLFG 463
The following BLAST results are available for this feature:
BLAST of BED-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
B0W9I73.795e-5332.07Predicted protein OS=Culex quinquefasciatus OX=717... [more]
A0A0N7Z9Z34.617e-5032.40BED-type domain-containing protein OS=Scylla oliva... [more]
A0A226D4I74.885e-4730.32Putative AC transposase OS=Folsomia candida OX=158... [more]
A0A4C1Y4861.049e-4534.29BED-type domain-containing protein OS=Eumeta varie... [more]
A0A226DDR71.117e-4126.46Putative AC9 transposase OS=Folsomia candida OX=15... [more]
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BLAST of BED-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of BED-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000077975.13.869e-6331.56SMESG000077975.1[more]
SMESG000026901.11.548e-6231.59SMESG000026901.1[more]
SMESG000061313.14.955e-6030.48SMESG000061313.1[more]
SMESG000022344.15.085e-6029.95SMESG000022344.1[more]
SMESG000065760.12.891e-5832.28SMESG000065760.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018950 ID=SMED30018950|Name=BED-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2209bp
ATTTGAACTATCCCAAAGTTTCTTGTGATGGAACTGTTGGAATTCTCCTT
TTTCAGTTCAATTTCTAGTTTTAATCCGTGTTGTCATAACGTATATTTCA
TATTTAAATACAATTATGTAAATAGTAGTTGTAAGCCCCTCAATAGAAAA
TAAATAGTTACTTTCTGGAAGTCGACAAGAAAGGTCGTCTTCATTACTTA
CAAGCCGTATGGCATTTCCTGTGTCTATATTACTATATAAAACGTTTGGA
GTTTAAATGCGTGAAATTATTAAAGCCGGCGGCATTTGAGTGATTTCATT
ACTATTTAAAAGTGAGTAAACATTAGGAAATTGTAAAATAAAGAAGAAAT
AAAGAAAAAACTTAACCTCATCAGAGTACAAAATTAACTAGAAAAAGTGT
ATTTGGTATATTTTTTATTCATATAATTGCTAATGAAATATATACATATT
TTATTATTTAGACAACATAATGGCATCAAATGAGAAGGAATCGTGGGTTT
GGAAATACTTTATCAAACGAATTGTTAACAGAAATACTGTCGGTGCAAAA
TGTACAATTTGTAAAAATGAAAAGGATTTAGTTGCTAACACTTCTACTTT
GGCTCGACATCTTACTTCTATTCACAACATTAACATCAAATCGGCTCATC
TAGACAGCACAATTATAGCTACGCAGAGAGTTCAAACAAATGTTATTTCG
TTTTTGCAGCGAAAAACATTAGAATATCTTATCGCCGAGCTAGCAGCTGT
TGATGGTTTTTCAATTCGAGGCATAACAAATTCAAAATTTATTCGCGAAA
GCATTACTGCGAAAGGTTATCACTTGCCAAGATGCGAAACTCAAGTCATG
GGTCTTGTGCACAAATTTTATGATCAAGTAAAGGAAGCTGCAATACAACA
GATTATTGACGATAAATCAGAAAATTTAAAGTTTTCCGTTACTCTCGATG
AATAGGTTGATGCCACAGCACGGAAATTTTTCAATGTCACATTAAATCGA
TCACGCAACGGTGTAATTAATCTAGGACTAGTGAGAATAATTGGAAGCTG
TACAGCTGAAAAATTATTTGAAGCTGTTAATAATCATTTATTGAGTTTTG
GGATCGACTTCACACGCGACATTGTAGCTGCAACTGGTGATGGAGCAAGT
ACGATGTTGAAATTTGGCAGATTAAGTCCCGTCATATATCAAATCTGTCT
TAATCATGCTTTTCATCTGGGCGTCATCAAAACTTTCTATCATAAAAAGG
AAAGATTACTTGATATAAATCAAGCAATTGAAACTGAAGTTCTTGAGGAT
AACTTTAATGAAGATGAATATCCTATGGAAGGTGACGAAGACGAAGAATT
TTATCTCAACGAAGATATTGAATCAATGTTAGCTTCTGTCCGTAGAATTG
TAGTGTTATTCAGACGATCTTCACTCAAAACTGATCTTTTACATAAGAAA
ATACAAGAACTGAATGTCGTCACTGAAAATGATATCAGTGCAGAAGATGA
ACTTCAAAAGAAACAACTTGATGAAAAAGTTAAGCTCCAACTTGATGTGA
AGATAAGATGGAATACAATCGTCCACATGATAAGAATTTTCATAAAATAT
TATGAACCAATTAAAGATGCATTGATAGAGCTGGATGAAGAAAGAATGTT
GTATGGTATTAATAAAGAAAACCTTCAAATGCTTATCAACATCCTTACTC
CTATTGAATTAGCAGTATTGGAATTAGGAAAGCGAGATGCAACTCTTCGA
AAAGCAATGGTTTCTATTGAATTTGTTCGAGATAAAATATCACAAAACAC
TTCGGCTATTAGTTTGAAATTATTGCAAAATATAAATGAATATATTGATG
ATAGAATGGATCCAAACCTTTTTCATCTCATCGAATGCCTTGACACTGCT
GCTGTACCACCGAATTCTGTTCTAAAATTTGCTACAGAGATAATGAAACG
ACTTTTTGGCACGGATGAGACAAATGATGAAAATGAATCTGATAATATTC
TGGCAACAGTTTCACGTGAGGATAATAATTTTTCCAGTCTGGCTCAAGAG
TTAACACAACGTATTAATTCGTCTTCGATTCCAAAAACATTACAGTTGAA
ATTTGATAAGTTAAAACAAGAATTCCAAATTTTTAAATCGACTGGAAATA
GAACAGAAAATCTAGAAAGACTACGAAATGCCCTGGACACAATTCAACCG
ACATCAACT
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protein sequence of SMED30018950-orf-1

>SMED30018950-orf-1 ID=SMED30018950-orf-1|Name=SMED30018950-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=352bp
MLKFGRLSPVIYQICLNHAFHLGVIKTFYHKKERLLDINQAIETEVLEDN
FNEDEYPMEGDEDEEFYLNEDIESMLASVRRIVVLFRRSSLKTDLLHKKI
QELNVVTENDISAEDELQKKQLDEKVKLQLDVKIRWNTIVHMIRIFIKYY
EPIKDALIELDEERMLYGINKENLQMLINILTPIELAVLELGKRDATLRK
AMVSIEFVRDKISQNTSAISLKLLQNINEYIDDRMDPNLFHLIECLDTAA
VPPNSVLKFATEIMKRLFGTDETNDENESDNILATVSREDNNFSSLAQEL
TQRINSSSIPKTLQLKFDKLKQEFQIFKSTGNRTENLERLRNALDTIQPT
ST
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protein sequence of SMED30018950-orf-2

>SMED30018950-orf-2 ID=SMED30018950-orf-2|Name=SMED30018950-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp
MASNEKESWVWKYFIKRIVNRNTVGAKCTICKNEKDLVANTSTLARHLTS
IHNINIKSAHLDSTIIATQRVQTNVISFLQRKTLEYLIAELAAVDGFSIR
GITNSKFIRESITAKGYHLPRCETQVMGLVHKFYDQVKEAAIQQIIDDKS
ENLKFSVTLDE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
IPR003656Znf_BED
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0003677DNA binding
GO:0016301kinase activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation