C2H2-type domain-containing protein

Overview
NameC2H2-type domain-containing protein
Smed IDSMED30018905
Length (bp)1701
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of C2H2-type domain-containing protein (SMED30018905) t-SNE clustered cells

Violin plots show distribution of expression levels for C2H2-type domain-containing protein (SMED30018905) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of C2H2-type domain-containing protein (SMED30018905) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for C2H2-type domain-containing protein (SMED30018905) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018905

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
tail regionSMED30018905SMESG000037063.1 SmedASXL_010240SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30018905SMESG000037063.1 dd_Smed_v4_34785_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30018905SMESG000037063.1 dd_Smed_v4_34785_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30018905SMESG000037063.1 dd_Smed_v4_34785_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Match: die-1 (Dorsal Intercalation and Elongation defect [Source:UniProtKB/TrEMBL;Acc:A0A131MCL4])

HSP 1 Score: 48.9062 bits (115), Expect = 9.340e-6
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:  804 CDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGI 902
            CDIC K++CNKYFLRTH  K+HGI  D  K+ I
Sbjct:  316 CDICNKEVCNKYFLRTHMLKMHGIVIDENKTVI 348
[Close]
BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Match: die-1 (Dorsal Intercalation and Elongation defect [Source:UniProtKB/TrEMBL;Acc:A0A131MCL4])

HSP 1 Score: 48.9062 bits (115), Expect = 9.340e-6
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 3 
Query:  804 CDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGI 902
            CDIC K++CNKYFLRTH  K+HGI  D  K+ I
Sbjct:  316 CDICNKEVCNKYFLRTHMLKMHGIVIDENKTVI 348
[Close]
BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Match: CG18265 (gene:FBgn0036725 transcript:FBtr0333689)

HSP 1 Score: 50.447 bits (119), Expect = 5.135e-6
Identity = 39/167 (23.35%), Postives = 70/167 (41.92%), Query Frame = 3 
Query:  768 SMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFE-----LNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGI 1253
            +  A+     V CDIC K   N + +R HR K H  ++    +  +  N   P++        N     + K+    D++   +   E       L+ +   F+    K P S +     +  +  D  +   ++ P      C++C R   N+YFL+THK+K+HG+
Sbjct:  462 AGGASNPVASVHCDICSKRFTNIFAMRRHRAKQHEAQA----AAQVSPNSQTPMQSQSHRGSPNEPSQPTIKQEMSQDAEIKPYQLPEGFREDFTLEQEELSFVPPPRKLP-SHLQQQAKDSNFNPDKLRRLGVQFP---DFFCELCCREYANRYFLRTHKWKRHGL 620
[Close]
BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Match: CG18265 (gene:FBgn0036725 transcript:FBtr0333688)

HSP 1 Score: 50.447 bits (119), Expect = 5.135e-6
Identity = 39/167 (23.35%), Postives = 70/167 (41.92%), Query Frame = 3 
Query:  768 SMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFE-----LNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGI 1253
            +  A+     V CDIC K   N + +R HR K H  ++    +  +  N   P++        N     + K+    D++   +   E       L+ +   F+    K P S +     +  +  D  +   ++ P      C++C R   N+YFL+THK+K+HG+
Sbjct:  462 AGGASNPVASVHCDICSKRFTNIFAMRRHRAKQHEAQA----AAQVSPNSQTPMQSQSHRGSPNEPSQPTIKQEMSQDAEIKPYQLPEGFREDFTLEQEELSFVPPPRKLP-SHLQQQAKDSNFNPDKLRRLGVQFP---DFFCELCCREYANRYFLRTHKWKRHGL 620
[Close]
BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Match: CG18265 (gene:FBgn0036725 transcript:FBtr0075219)

HSP 1 Score: 50.447 bits (119), Expect = 5.135e-6
Identity = 39/167 (23.35%), Postives = 70/167 (41.92%), Query Frame = 3 
Query:  768 SMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFE-----LNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGI 1253
            +  A+     V CDIC K   N + +R HR K H  ++    +  +  N   P++        N     + K+    D++   +   E       L+ +   F+    K P S +     +  +  D  +   ++ P      C++C R   N+YFL+THK+K+HG+
Sbjct:  462 AGGASNPVASVHCDICSKRFTNIFAMRRHRAKQHEAQA----AAQVSPNSQTPMQSQSHRGSPNEPSQPTIKQEMSQDAEIKPYQLPEGFREDFTLEQEELSFVPPPRKLP-SHLQQQAKDSNFNPDKLRRLGVQFP---DFFCELCCREYANRYFLRTHKWKRHGL 620
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A267DC64 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017093g1 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 6.082e-22
Identity = 61/186 (32.80%), Postives = 92/186 (49.46%), Query Frame = 3 
Query:  732 ILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSN 1289
            + S++ LDAF+ SM AAKLADRV CD+C K++CNKYFL+TH++KVHGI  +L   G                    +RP          D  S  W Q                +    ++   + ++   +    K +    P   +++CDIC + +CNKYFL+ H+  KHGI D ++S P N++
Sbjct:  289 LASAHHLDAFQSSMVAAKLADRVICDVCHKEVCNKYFLKTHKEKVHGIPRELQAGG--------------------SRPGQQ-------DGSSGKWPQLPATTAVATTTAAAAAAAAAAAAAASAAASAAGSGHPGK-QSSGSPAQQRIRCDICGKEVCNKYFLRVHRLNKHGITDSSVSSPANTD 446
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A267DQV4 (C2H2-type domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001630g1 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 8.319e-22
Identity = 61/186 (32.80%), Postives = 92/186 (49.46%), Query Frame = 3 
Query:  732 ILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSN 1289
            + S++ LDAF+ SM AAKLADRV CD+C K++CNKYFL+TH++KVHGI  +L   G                    +RP          D  S  W Q                +    ++   + ++   +    K +    P   +++CDIC + +CNKYFL+ H+  KHGI D ++S P N++
Sbjct:  517 LASAHHLDAFQSSMVAAKLADRVICDVCHKEVCNKYFLKTHKEKVHGIPRELQAGG--------------------SRPGQQ-------DGGSGKWPQLPATTAVATTTAAAAAAAAAAAAAASAAASAAGSGHPGK-QSSGSPAQQRIRCDICGKEVCNKYFLRVHRLNKHGITDSSVSSPANTD 674
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A267DRZ5 (C2H2-type domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015192g2 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 4.422e-21
Identity = 60/186 (32.26%), Postives = 92/186 (49.46%), Query Frame = 3 
Query:  732 ILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSN 1289
            + S++ LDAF+ SM AAKLADRV CD+C K++CNKYFL+TH++KVHGI  +L   G                    +RP   +         S  W Q                +    ++   + ++   +    K +    P   +++CDIC + +CNKYFL+ H+  KHGI D ++S P N++
Sbjct:  716 LASAHHLDAFQSSMVAAKLADRVICDVCHKEVCNKYFLKTHKEKVHGIPRELQAGG--------------------SRPGQQD--------GSGKWPQLPATTAVATTTAAAAAAAAAAAAAASAAASAAGSGHPGK-QSSGSPAQQRIRCDICGKEVCNKYFLRVHRLNKHGITDSSVSSPANTD 872
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A267EUH2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015192g1 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 3.642e-19
Identity = 55/173 (31.79%), Postives = 82/173 (47.40%), Query Frame = 3 
Query:  771 MAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSN 1289
            M AAKLADRV CD+C K++CNKYFL+TH++KVHGI  +L   G                    +RP          D  S  W Q                +    ++   + ++   +    K +    P   +++CDIC + +CNKYFL+ H+  KHGI D ++S P N++
Sbjct:    1 MVAAKLADRVICDVCHKEVCNKYFLKTHKEKVHGIPRELQAGG--------------------SRPGQQ-------DGSSGKWPQLPATTAVATTTAAAAAAAAAAAAAASAAASAAGSGHPGK-QSSGSPAQQRIRCDICGKEVCNKYFLRVHRLNKHGITDSSVSSPANTD 145
[Close]
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A210QCT5 (Zinc finger and BTB domain-containing protein 40 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09292 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.030e-18
Identity = 103/416 (24.76%), Postives = 174/416 (41.83%), Query Frame = 3 
Query:  135 NNEDFCSICQKHFCNKYYLKKHKLDVHKTLLPDSTEMNISHNSSALLNENFSSNHFCNLDINYIKYLFNQMSERLKKETEIHDSNFFNSINYKEIMSKVNQMDPAYQFHSEVHDSSLMKPSPAAGDLGFCRLNFSNLPLSWHICFQCNLRFPDELSLSNHSHTVHRIDANHG---NGISDA-QKQFFQNIQFLSNQ------------------TTNQIDEILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKF----IKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKP---------LANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLP 1277
            N E +C +C+K FCNKY+LK HK + H   L D   + +      L    F       +  + I       S  +  ET +  SN           S++N+M         V   S  KP  +       + N+         C  CN  F      S +++ +HRI   HG    G+  +  ++F +++  +  +                  T N++ E    N +     +M AAKLADRV+CDIC K+LCNKYFL+ H+ KVHG+ +  ++       ++  +   +  + KN +P   + +      + +H N     + S+ + F     +L S  P+  +P +    +   D      + KP         +  +  C+IC +  C+KYFL+TH+   HGI+++    P
Sbjct:  514 NAEAYCELCRKEFCNKYFLKVHKANKHGMYL-DEFPLGVPVGPVGLPGTPF-------IPASSID------STAITSETPVSYSN-----------SELNKM--------YVKPESSPKPGTSLVTSSASQDNYVTY------CNLCNQEF-----ASKYTYRIHRIQV-HGMLHEGLEGSLTEEFMRSVNSMREKEEAMAKISPGSRMVEEPHTENKVPE----NGMTTMFGNMVAAKLADRVTCDICNKELCNKYFLKVHKYKVHGVGTPSVERQ---EKLTKRMFVGDFETIKNGKPIAPKMR----SERPHHANP----VKSEPKDFSLHSPQLPSSLPLPVLPKA---SEVPTDLSPSAILEKPSHDELVKMGIDPEAYCEICKKEFCSKYFLRTHRLNIHGIRNEKSESP 866
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000037063.1 (SMESG000037063.1)

HSP 1 Score: 1048.88 bits (2711), Expect = 0.000e+0
Identity = 530/533 (99.44%), Postives = 531/533 (99.62%), Query Frame = 3 
Query:   57 MSKNKLSNEEIYPRCLIMENEKSNFNNNEDFCSICQKHFCNKYYLKKHKLDVHKTLLPDSTEMNISHNSSALLNENFSSNHFCNLDINYIKYLFNQMSERLKKETEIHDSNFFNSINYKEIMSKVNQMDPAYQFHSEVHDSSLMKPSPAAGDLGFCRLNFSNLPLSWHICFQCNLRFPDELSLSNHSHTVHRIDANHGNGISDAQKQFFQNIQFLSNQTTNQIDEILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSNEINYKISFDSDIITSKNHLTTCTSSINSIPNILNYLDYRITDKNEQNDNKSSYHNMILLQKEEPLDLSLPKQALLVHSRSTFYKTNINQKNKLNIRXXXXXXXXXXXXXFYRNLISKYLGLK 1655
            MSKNKLSNEEIYPRCLIMENEKSNFNNNEDFCSICQKHFCNKYYLKKHKLDVHKTLLPDSTEMNISHNSSALLNENFSSNHFCNLDINYIKYLFNQMSERLKKETEIHDSNFFNSINYKEIMSKVNQMDPAYQFHSEVHDSSLMKPSP AG+LGFCRLNFSNLPLSWHICFQCNLRFPDELSLSNHSHTVHRIDANHGNGISDAQKQFFQNIQFLSNQTTNQIDEILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSN WNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSNEINYKISFDSDIITSKNHLTTCTSSINSIPNILNYLDYRITDKNEQNDNKSSYHNMILLQKEEPLDLSLPKQALLVHSRSTFYKTNINQKNKLNIRKKLSSLKSTKNNTFYRNLISKYLGLK
Sbjct:    1 MSKNKLSNEEIYPRCLIMENEKSNFNNNEDFCSICQKHFCNKYYLKKHKLDVHKTLLPDSTEMNISHNSSALLNENFSSNHFCNLDINYIKYLFNQMSERLKKETEIHDSNFFNSINYKEIMSKVNQMDPAYQFHSEVHDSSLMKPSPTAGELGFCRLNFSNLPLSWHICFQCNLRFPDELSLSNHSHTVHRIDANHGNGISDAQKQFFQNIQFLSNQTTNQIDEILSSNTLDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNPWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSNEINYKISFDSDIITSKNHLTTCTSSINSIPNILNYLDYRITDKNEQNDNKSSYHNMILLQKEEPLDLSLPKQALLVHSRSTFYKTNINQKNKLNIRKKLSSLKSTKNNTFYRNLISKYLGLK 533
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000004456.1 (SMESG000004456.1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.918e-18
Identity = 60/170 (35.29%), Postives = 80/170 (47.06%), Query Frame = 3 
Query:  750 LDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVK--CDICDRLICNKYFLKTHKYKKHGI 1253
             DAFK SMAAAKLADRV+CDIC KDLCNKYFLRTH+ K+HG+++                  ++      NR    ++K    D   N  +Q   N       F +     P+       +   +T +SK      +P + +    C IC RLI   YFL  H +  HGI
Sbjct:  361 FDAFKSSMAAAKLADRVTCDICHKDLCNKYFLRTHKLKLHGVQT------------------IDSIGRTGNR----KEKTGFFDRCQNSMDQLNPNPGFIGTPFSRNPLPDPL------LTASLFTNNSKP-----RPGSPETGCFCPICKRLISLPYFLPLHMWNAHGI 497

HSP 2 Score: 71.633 bits (174), Expect = 8.731e-13
Identity = 61/188 (32.45%), Postives = 81/188 (43.09%), Query Frame = 3 
Query:  804 CDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIK--LESKYPVSSMPHSFSNKQYTFDSKKCKWIRK-PLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSNEINYKISFDSDIITSKNHLTTCT 1358
            C IC + +   YFL  H    HGI       G+  H    P                     S+  S+SN      L+ +     FI   L+SK   S  P SF N          KW RK P   +V+CDICD+ ICNKYFL+THK  KHG+ D   +  +NS+ ++     DS   T+ N  +T T
Sbjct:  474 CPICKRLISLPYFLPLHMWNAHGI-------GMGSHTSIMP---------------------SVCSSRSN------LDQNILEPPFIPNNLQSKINPSLFPLSFCNNNID-TGMSSKWYRKSPNQMRVRCDICDKWICNKYFLRTHKLNKHGLTDNGSNTDVNSDSVSRNFFIDSS--TAPNPSSTGT 624

HSP 3 Score: 57.7658 bits (138), Expect = 1.945e-8
Identity = 24/38 (63.16%), Postives = 28/38 (73.68%), Query Frame = 3 
Query:  108 MENEKSNFNNNEDFCSICQKHFCNKYYLKKHKLDVHKT 221
            +ENE    +  EDFC  CQK FCNKYYL+KHK+DVHK 
Sbjct:   83 LENEPDKNSEEEDFCEKCQKRFCNKYYLRKHKIDVHKV 120

HSP 4 Score: 55.4546 bits (132), Expect = 9.231e-8
Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 3 
Query: 1098 PHSFSNKQY--TFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQ 1256
            P + +N  Y   FD+ K       LA++V CDIC + +CNKYFL+THK K HG+Q
Sbjct:  349 PVNTTNDSYISPFDAFKSSMAAAKLADRVTCDICHKDLCNKYFLRTHKLKLHGVQ 403
[Close]
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000004456.1 (SMESG000004456.1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.924e-18
Identity = 60/170 (35.29%), Postives = 80/170 (47.06%), Query Frame = 3 
Query:  750 LDAFKYSMAAAKLADRVSCDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHSFSNKQYTFDSKKCKWIRKPLANKVK--CDICDRLICNKYFLKTHKYKKHGI 1253
             DAFK SMAAAKLADRV+CDIC KDLCNKYFLRTH+ K+HG+++                  ++      NR    ++K    D   N  +Q   N       F +     P+       +   +T +SK      +P + +    C IC RLI   YFL  H +  HGI
Sbjct:  363 FDAFKSSMAAAKLADRVTCDICHKDLCNKYFLRTHKLKLHGVQT------------------IDSIGRTGNR----KEKTGFFDRCQNSMDQLNPNPGFIGTPFSRNPLPDPL------LTASLFTNNSKP-----RPGSPETGCFCPICKRLISLPYFLPLHMWNAHGI 499

HSP 2 Score: 71.633 bits (174), Expect = 8.518e-13
Identity = 61/188 (32.45%), Postives = 81/188 (43.09%), Query Frame = 3 
Query:  804 CDICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEKNNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIK--LESKYPVSSMPHSFSNKQYTFDSKKCKWIRK-PLANKVKCDICDRLICNKYFLKTHKYKKHGIQDQTLSLPINSNEINYKISFDSDIITSKNHLTTCT 1358
            C IC + +   YFL  H    HGI       G+  H    P                     S+  S+SN      L+ +     FI   L+SK   S  P SF N          KW RK P   +V+CDICD+ ICNKYFL+THK  KHG+ D   +  +NS+ ++     DS   T+ N  +T T
Sbjct:  476 CPICKRLISLPYFLPLHMWNAHGI-------GMGSHTSIMP---------------------SVCSSRSN------LDQNILEPPFIPNNLQSKINPSLFPLSFCNNNID-TGMSSKWYRKSPNQMRVRCDICDKWICNKYFLRTHKLNKHGLTDNGSNTDVNSDSVSRNFFIDSS--TAPNPSSTGT 626

HSP 3 Score: 57.7658 bits (138), Expect = 1.913e-8
Identity = 24/38 (63.16%), Postives = 28/38 (73.68%), Query Frame = 3 
Query:  108 MENEKSNFNNNEDFCSICQKHFCNKYYLKKHKLDVHKT 221
            +ENE    +  EDFC  CQK FCNKYYL+KHK+DVHK 
Sbjct:   85 LENEPDKNSEEEDFCEKCQKRFCNKYYLRKHKIDVHKV 122

HSP 4 Score: 55.4546 bits (132), Expect = 9.238e-8
Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 3 
Query: 1098 PHSFSNKQY--TFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQ 1256
            P + +N  Y   FD+ K       LA++V CDIC + +CNKYFL+THK K HG+Q
Sbjct:  351 PVNTTNDSYISPFDAFKSSMAAAKLADRVTCDICHKDLCNKYFLRTHKLKLHGVQ 405
The following BLAST results are available for this feature:
BLAST of C2H2-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
die-19.340e-663.64Dorsal Intercalation and Elongation defect [Sourc... [more]
die-19.340e-663.64Dorsal Intercalation and Elongation defect [Sourc... [more]
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BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CG182655.135e-623.35gene:FBgn0036725 transcript:FBtr0333689[more]
CG182655.135e-623.35gene:FBgn0036725 transcript:FBtr0333688[more]
CG182655.135e-623.35gene:FBgn0036725 transcript:FBtr0075219[more]
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BLAST of C2H2-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DC646.082e-2232.80Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DQV48.319e-2232.80C2H2-type domain-containing protein OS=Macrostomum... [more]
A0A267DRZ54.422e-2132.26C2H2-type domain-containing protein (Fragment) OS=... [more]
A0A267EUH23.642e-1931.79Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A210QCT52.030e-1824.76Zinc finger and BTB domain-containing protein 40 O... [more]
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BLAST of C2H2-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000037063.10.000e+099.44SMESG000037063.1[more]
SMESG000004456.13.918e-1835.29SMESG000004456.1[more]
SMESG000004456.13.924e-1835.29SMESG000004456.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018905 ID=SMED30018905|Name=C2H2-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1701bp
TTTTTTCTGAATTTTAAATTAATTGAAGAGAGACAGTTATATATTTTCGG
TTGATTATGTCAAAAAATAAATTATCAAACGAAGAGATTTATCCTCGATG
TTTAATAATGGAAAATGAAAAATCCAATTTTAACAACAATGAAGATTTCT
GTTCAATTTGCCAAAAACATTTTTGCAATAAATATTATCTGAAAAAACAC
AAACTCGATGTTCACAAAACTCTCTTGCCCGACTCGACTGAAATGAACAT
TTCCCATAATTCGAGTGCTTTATTAAATGAAAATTTCTCTTCCAATCATT
TCTGCAATCTTGACATTAATTACATTAAGTATCTTTTCAATCAGATGTCC
GAAAGATTGAAAAAAGAAACAGAAATCCACGATTCCAATTTTTTCAATTC
AATCAACTACAAAGAAATAATGTCAAAAGTAAATCAAATGGATCCTGCTT
ACCAATTCCATTCAGAAGTCCACGATTCGTCGTTGATGAAACCGAGCCCA
GCTGCTGGGGATCTCGGATTTTGTCGTTTGAATTTCTCTAATTTGCCTTT
GTCATGGCATATTTGTTTTCAATGCAACTTACGGTTCCCCGATGAATTAA
GTCTTTCAAATCACTCTCATACTGTTCACAGAATCGACGCTAACCACGGA
AATGGGATTTCTGATGCGCAAAAACAATTCTTTCAAAACATACAATTCCT
TTCCAATCAAACCACAAACCAAATCGACGAGATTTTGTCTTCAAATACTT
TAGACGCTTTCAAATACAGCATGGCAGCAGCTAAGTTGGCTGACAGAGTT
AGTTGTGATATTTGCGGGAAAGATTTATGCAACAAATACTTTCTCAGAAC
TCACCGGAAAAAGGTTCACGGAATCAAAAGCGATTTATTAAAATCTGGAA
TTATTTGTCATAACATCTCATATCCTTTGGAAAGATTGGAACTTAATTCA
GAGAAAAATAACCGCCCAAATCATTCAGAAAAGAAATTATCAATCGGTGA
TTCAAAATCAAATCATTGGAACCAATTTGAACTTAATCTTGATTCCGATC
GTAGAAAATTCATCAAACTTGAGTCGAAATATCCAGTTTCCTCGATGCCG
CATTCGTTTTCTAATAAACAATACACGTTCGATTCCAAGAAATGCAAATG
GATACGGAAACCTTTGGCAAACAAAGTAAAATGTGACATTTGTGATCGGT
TGATTTGTAACAAATATTTTTTAAAAACTCATAAATATAAGAAACATGGA
ATTCAAGACCAAACTTTGTCACTTCCAATAAATTCAAACGAAATAAATTA
TAAAATATCTTTTGATTCTGACATCATTACCTCCAAAAACCACTTAACGA
CATGCACTTCGTCCATAAACTCTATCCCAAATATTTTGAATTATCTCGAT
TATAGAATTACAGATAAAAATGAACAAAATGATAACAAATCATCATATCA
CAATATGATTTTACTTCAAAAGGAAGAGCCACTTGATTTGAGTCTTCCGA
AACAAGCTCTATTAGTTCATTCAAGAAGTACTTTTTATAAAACTAACATA
AACCAAAAAAACAAATTAAATATCAGAAAAAAATTATCATCATTAAAATC
TACAAAAAACAATACATTTTATAGAAATCTGATCAGCAAATATTTGGGTT
TAAAAAAAATTCGATTTTTCTGCCGGGCCTGATCATTGAGATTTTACAGA
A
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protein sequence of SMED30018905-orf-1

>SMED30018905-orf-1 ID=SMED30018905-orf-1|Name=SMED30018905-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=542bp
MSKNKLSNEEIYPRCLIMENEKSNFNNNEDFCSICQKHFCNKYYLKKHKL
DVHKTLLPDSTEMNISHNSSALLNENFSSNHFCNLDINYIKYLFNQMSER
LKKETEIHDSNFFNSINYKEIMSKVNQMDPAYQFHSEVHDSSLMKPSPAA
GDLGFCRLNFSNLPLSWHICFQCNLRFPDELSLSNHSHTVHRIDANHGNG
ISDAQKQFFQNIQFLSNQTTNQIDEILSSNTLDAFKYSMAAAKLADRVSC
DICGKDLCNKYFLRTHRKKVHGIKSDLLKSGIICHNISYPLERLELNSEK
NNRPNHSEKKLSIGDSKSNHWNQFELNLDSDRRKFIKLESKYPVSSMPHS
FSNKQYTFDSKKCKWIRKPLANKVKCDICDRLICNKYFLKTHKYKKHGIQ
DQTLSLPINSNEINYKISFDSDIITSKNHLTTCTSSINSIPNILNYLDYR
ITDKNEQNDNKSSYHNMILLQKEEPLDLSLPKQALLVHSRSTFYKTNINQ
KNKLNIRKKLSSLKSTKNNTFYRNLISKYLGLKKIRFFCRA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000421tail
Vocabulary: INTERPRO
TermDefinition
IPR013087Znf_C2H2_type
IPR015880Znf_C2H2-like
IPR007087Znf_C2H2
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 168..191
e-value: 0.67
score: 19.0
coord: 30..53
e-value: 13.0
score: 12.2
coord: 248..271
e-value: 0.6
score: 19.2
coord: 374..397
e-value: 6.9
score: 14.7
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 170..191
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 376..397
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 32..53
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 250..271
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 248..271
score: 8.912
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 30..58
score: 8.891
NoneNo IPR availablePANTHERPTHR21190UNCHARACTERIZEDcoord: 130..469