SMED30018898

Overview
NameSMED30018898
Smed IDSMED30018898
Length (bp)3039
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018898 (SMED30018898) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018898 (SMED30018898) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018898 (SMED30018898) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018898 (SMED30018898) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018898

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30018898SMESG000079137.1 SmedASXL_013893SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
headSMED30018898SMESG000079137.1 dd_Smed_v6_17993_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
headSMED30018898SMESG000079137.1 OX_Smed_1.0.14151ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30018898 vs. TrEMBL
Match: V4A082 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_239156 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 7.996e-28
Identity = 133/520 (25.58%), Postives = 240/520 (46.15%), Query Frame = 2
Query:  296 FHCNHKLSIQTHINDLIQNYRKIQELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQ-ILTT---KLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIII---------EHSLYQKRFKLTRDLY-MNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDE--IKLFRN------------KSRNQKSNR--------QLXXXXXXXXXXNRCLSS-LKQIG-----------SPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVI--PIEQPSSCPFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLEIE-KMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSI 1702
             HC    ++  H  +L +       +E   + +S+++     +E ++ L+  E++     R   +     S+L CL ALCE+LRI +N+  S+   +  NK LQS L     FL   +D  + +LTT   K I    + + TG  V +H D+ + T + LW ++RG+EE+N I          E S   +    +++L+  +  H + + ++   ++   + GI    FSK+L  LA ERS   A   H +F      ++E  +K+ ++            +++NQKS++        Q       +   N  LS+ L  IG           SP+ D+ R E EFT NFL ++  ST +L++   + S +K+ ++D    F  PV+  P    S  P +  ++   RK V W D+ D S+R+Q++ +Y++++W      L        +  H   ++ + GS L+ N +    +  +++ +   +IFP G V  LL  A  +   +    WD  +C ++
Sbjct:   99 IHCPRSPALACHYGELRKLMENRARIEFVRDCMSKIRAAAAYVEELEGLVHCEYRTAYAIRHGCASEAPVSKLYCLNALCEDLRIHVNHWNSIKQKLNSNKWLQSNL----GFLSMEVDYVKRMLTTLCDKAIWWLDKLIITGFEVFAHCDMDKLTPEVLWNVTRGLEEFNTIALSLRSVSVSECSTILQALLPSKNLFSTSCLHLDPSFSNFPCNLSHSVKGI---PFSKVLTLLAKERSRYAASLTHRFF-----TVNEEFVKILKSGHLPVYLWNTDQENQNQKSSQMSKNHMIPQDTSDYHTETGSNTSLSATLLSIGSIHAPDLSIYMSPLVDFSRREQEFTENFLLVVCNSTNLLRK---NESTKKVRQKDGKATFS-PVVGRPPRVQSDMPVVLSRSDSRRKTVSWGDNADSSVRNQIVSKYMDIMWACFGQNLDLCLDEPSWEIHKNVLKSEFGSVLLSNDTLIAVLRHMMEHVCIKDIFPQGSVQPLLKVAHRLTNLSAYSAWDTALCNAV 602          
BLAST of SMED30018898 vs. TrEMBL
Match: A0A0R3WD62 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS8691 PE=4 SV=2)

HSP 1 Score: 118.627 bits (296), Expect = 1.422e-23
Identity = 120/410 (29.27%), Postives = 180/410 (43.90%), Query Frame = 2
Query:  368 ELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQ----KRFKLTRDLYMNINHYEVTLNSVNKSIDQQLF----------GIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIKLFRNKSRNQKSNRQLXXXXXXXXXXNRCLSSLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKEL--------------------------SNSVSKRKLC---KEDTVDNFMVPVI--PIEQPSSCPFLNVKTC-----KPRKGVQWSDHKDVSLRHQVIGQYLNVVW 1447
            EL  F N ++  QE+ N+      L+ +       SR   +I+ + +R+  +R +CE LR  L    SL S  + N  L S L  Y   L     RY ++   ++  +   V +GLL+LS SDL RYTH +LWEI+RGIEE +++IEH   Q    K+F+L R L       E  L+S+N  ++Q+L            +R  S   I+Y LA  RS   A  +     QS                                    L   K     + + ++ EYEF SNFLD+++QSTE+L+ L                           NS   RKL    K +++D F+      P ++  S   +          + +K VQWSD+ +V+LRHQV+G+YL + W
Sbjct:   47 ELSQFVNQMAE-QEYANVSAGATGLM-NAFTRSSNSRRVSNINPTKTRMSSVRGICEGLRQGL----SLVS--RTNTLLASELGDYAP-LPPRTHRYTLVVVSVLIAASHIVNSGLLLLSQSDLTRYTHAELWEITRGIEELSMLIEHVQEQLDEKKQFQLRRLLL-----DECPLSSINVPLEQRLSELSLLTDPIDSLRPASVGHIIYLLARYRSSTPARILVGDLLQS------------------------------------LECPKGRIDFLAEVVQKEYEFCSNFLDVMTQSTELLRNLRRVQETVDPRPKSILHRTSPISSVQCKNSRICRKLVHVTKTESIDEFISAFSEKPSKETKSSALIEEGDEEGGEKQQKKVVQWSDYYEVALRHQVVGRYLQLTW 406          
BLAST of SMED30018898 vs. TrEMBL
Match: K1QA83 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10012394 PE=4 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.676e-21
Identity = 110/509 (21.61%), Postives = 218/509 (42.83%), Query Frame = 2
Query:  278 KYDKMDF-HCNHKLSIQTHINDLIQNYRKIQELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKLTRDLYMNINHYEVTLNSVNKSIDQQL--------FGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIK-LFRNKSRNQKSNRQLXXXX------------XXXXXXNRCLSSLKQIGS-----------PVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVIPIEQPSSCPFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLE-IEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSI 1702
            +Y  + F HC H   +     +L     +   LE   + L R++     +  +++L+  E++          +    S++ CL ALCE+LR  + +  ++   +  N+ LQ  L +    LE        L +  I   ++ +  GL V +H  +   T + +W I+RG+E++N  + + L++ R+      Y  + H +  + ++    D ++        F ++++ FSKIL  +A+ER+   A   H +F  +    D ++ L+  K  N   N ++                           ++ ++GS           P+N++ R E EF  NFL I+  ST +L++  +S   +    +              +    P L+    K RK V W D  D S+R Q+  +Y++V+W+     L+   Y   +   S + + ++GS ++   +    +  +++ +   ++FPT  VP +L  A  +  +  L  WD  MC ++
Sbjct:   83 EYSSISFMHCRHLQGLGKQYMNLRSVMEERARLEFLRDCLFRIKSVTTFVHDLENLVQAEYRTYYAIMHNCLMDNPSSKIYCLNALCEDLRTHVGHWNNIKQCLHNNRWLQPILGRLYLDLEYVRRVLSQLYSDAIYWMEKLIVIGLQVFAHGSMATLTQEIVWNIARGLEDFN-NVANGLFKSRYMSIS--YFILEHLQSEMTNLTCKSDPRVTNHPGHVSFSVKSIPFSKILNIIAVERAKYAAIETHRFFTANE---DFVRILYSGKLPNYVWNDEVQYPGHISHHLDTSDYHTASGSMTSISGAILKVGSVRAPDLTPYEPPLNEFARHENEFAENFLLIVCNSTNLLRKGESSSKSKHKSGKSPRSPSSK----PPKGQDTPVLSRADSK-RKTVSWGDSADTSIRSQLTNRYMDVLWRFFGSALEQCFYEPLWGCRSNQMVGELGSVVLSGATVVTVVRNMMEHVCLKDMFPTNAVPPILGVARKLHAQTALQSWDILMCEAL 580          
BLAST of SMED30018898 vs. TrEMBL
Match: A0A3S5ALJ3 (Uncharacterized protein (Fragment) OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS16558 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.687e-21
Identity = 95/318 (29.87%), Postives = 152/318 (47.80%), Query Frame = 2
Query:  494 STSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYM---------DRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNII---IEHSLYQKRFKLTRDLYMNINHYEVTLNSVNKSIDQ-------------------QLFGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIKLFRNKSRNQKSNRQLXXXXXXXXXXNRCLSSLKQI----GSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSN-SVSKRKLCKEDTVDNFMVPVIPIEQPSSCP 1339
            S S S+L  LR LCENLR  L+   SL + ++ +     +L +  +  + Y+         D    L T  +  +   V +GL +L+H+DL +YTH++LWE++RG+EE  I+   ++ S   +R +  + L      YE  ++  +KS +Q                     F I ++S  +I Y LA +RS   A S+ +    SP+ I  I        NQ S RQL + +        C S +K +    G  + D+LR EYEF+S FLDI+SQSTEML+++    ++      E+ +    +P   I + S  P
Sbjct:  111 SASFSQLGFLRGLCENLRQGLSQANSLTNQLEQSFYQMRHLLRADSIFQPYIMISSRRLIEDSVVPLVTAALTAAHHLVHSGLNLLAHTDLTQYTHEELWEMARGMEELGILGTRLQVSQQNRRHQSIKSLL-----YESLISKGHKSEEQTKSTGKILALIEASGLFVDPAFSIGSISVGRIFYFLANQRSFATARSIRHKLLHSPVCISYI--------NQGS-RQLNDAEAL------CTSFVKYLQDSDGMLLEDFLRGEYEFSSGFLDILSQSTEMLRQIQKLQLASNATMAEEALIASQLPTYRIHKQSQKP 408          
BLAST of SMED30018898 vs. TrEMBL
Match: A0A2T7PA97 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_09604 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.448e-21
Identity = 118/523 (22.56%), Postives = 205/523 (39.20%), Query Frame = 2
Query:  299 HCNHKLSIQTHINDLIQNYRKIQELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNII--------IEHSLYQKRFKLTRDLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIKLFRN------------------------------KSRNQKSNRQLXXXXXXXXXXNRCLSSLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKE---------LSNSVSKRKLCKEDTVDNFMVPVIP-IEQPSSC----PFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLNVVWKLVN---DL-LKNTKYSNHYYSHSLEIEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTS 1699
            HC   + +  + + L +   +   LEL  + L R++     +E +++L+  E++     R    +    S+L CL ALCE+LR+   +  S+   +   +  Q  L  +   L         L  + I L    V  G  VL+H  L   T D LW I+RG+E++N I        +   +Y  +  L      ++  +    NS  +  D    GIR + F K+L  LA ERS   A   H +F  S   +  ++   +                               +       Q           +  +  L  + SP+ ++ + E +F  NFL I+  ST +L++           N    R   K  T  +F VP+ P + +P       P L+    + RK V W D+ D S+R QV+  Y++ +W+      DL L    ++N      L   ++GS  +   +    +  +++ I   +IFP+G +  LL     +   +    WD  M  +
Sbjct:   98 HCRRGVKLGGYYSQLRRLLEERAGLELLRDCLYRVRSAQLFVEELEALLSAEYRTAYAIRHGCLLDAPVSKLYCLNALCEDLRVHAGHWNSVKQRINTGRWAQPLLGFFCRELAAVRRTLGALCDRAIFLMDRLVHVGFEVLAHCALDALTADVLWNITRGLEDFNTIVVSFRAHVVAEGVYSPQSSLAARHLPSLYLHPALANSGRRLSD----GIRAVPFHKVLGILANERSRYAALLTHRFFTASDAFLAALRTATHLPPFEWEDDPVSSVSGVHLHSRFLSSPPPVAAGEAVGGSQASLNLALLHVGSVRVPDLSGLPSPLVEFSQKEQQFADNFLQIVCNSTSLLRKNDATSNNNSHHNHHHGRSKAKART--DFKVPLSPVVSRPPKAQGETPVLSRSDSR-RKTVSWGDNADTSIRTQVVATYMDTLWQHFGRNLDLFLDEPAWTNRV---GLLRSELGSIFLQRDTVCAMLRHMVEHICVKDIFPSGSISPLLGVVVRLHALSAFAAWDSNMTAA 610          
BLAST of SMED30018898 vs. Planmine SMEST
Match: SMESG000079137.1 (SMESG000079137.1)

HSP 1 Score: 1754.96 bits (4544), Expect = 0.000e+0
Identity = 907/909 (99.78%), Postives = 909/909 (100.00%), Query Frame = 2
Query:   89 MHASLGFIGRAQTIDSDELPMDDNAGSFSKSSTVNXXXXXXXXXXXXXXXXXLNYFSHSFLRFKYDKMDFHCNHKLSIQTHINDLIQNYRKIQELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKLTRDLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIKLFRNKSRNQKSNRQLXXXXXXXXXXNRCLSSLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVIPIEQPSSCPFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLEIEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSILLNHHMHSPDQILADNMSEISTKIYQHLQSQTNEFNNNNYKEKHVVKRENEHLEQVDPTVTIISVLTSTFQKYGEKLLSVSKSASNRLNSGEIAQLIWPTIANWAHLEMQFYSFLQSYGYFGSDINTSTSDKLRLISRNIPGLSLDNKISNLKKSLLEYYTIKCSERVNQLLDLGLMQKCTKFDSYNGGAQNLSPSAYAQGAILGVLEPIYRSIQFCSSYSQYDALSCTLEIFSSSWLKKLDIITKEKVFSEIQLQKDVGLIAEWILAKKSLSDSLKRELLRQESLVELENRVTMLVSVQDKSKSEIGFLQRQTSEMGLKVAKFYEELCSRSNSPNDQVSPNIXXXXXXXXXXXXXXXXGQAYFRSNKSDK 2815
            MHASLGFIGRAQTIDSDELPMDDNAGSFSKSSTVNSEEHKSIISSIHSKFISLNYFSHSFLRFKYDKMDFHCNHKLSIQTHINDLIQNYRKIQELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCS+VQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKLTRDLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIKLFRNKSRNQKSNRQLFEFQEDDFDFNRCLSSLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVIPIEQPSSCPFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLEIEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSILLNHHMHSPDQILADNMSEISTKIYQHLQSQTNEFNNNNYKEKHVVKRENEHLEQVDPTVTIISVLTSTFQKYGEKLLSVSKSASNRLNSGEIAQLIWPTIANWAHLEMQFYSFLQSYGYFGSDINTSTSDKLRLISRNIPGLSLDNKISNLKKSLLEYYTI+CSERVNQLLDLGLMQKCTKFDSYNGGAQNLSPSAYAQGAILGVLEPIYRSIQFCSSYSQYDALSCTLEIFSSSWLKKLDIITKEKVFSEIQLQKDVGLIAEWILAKKSLSDSLKRELLRQESLVELENRVTMLVSVQDKSKSEIGFLQRQTSEMGLKVAKFYEELCSRSNSPNDQVSPNISTKSNLSSFSSLLTKSGQAYFRSNKSDK
Sbjct:    1 MHASLGFIGRAQTIDSDELPMDDNAGSFSKSSTVNSEEHKSIISSIHSKFISLNYFSHSFLRFKYDKMDFHCNHKLSIQTHINDLIQNYRKIQELELFSNILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSFVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKLTRDLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFYQSPIVIDEIKLFRNKSRNQKSNRQLFEFQEDDFDFNRCLSSLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVIPIEQPSSCPFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLEIEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSILLNHHMHSPDQILADNMSEISTKIYQHLQSQTNEFNNNNYKEKHVVKRENEHLEQVDPTVTIISVLTSTFQKYGEKLLSVSKSASNRLNSGEIAQLIWPTIANWAHLEMQFYSFLQSYGYFGSDINTSTSDKLRLISRNIPGLSLDNKISNLKKSLLEYYTIRCSERVNQLLDLGLMQKCTKFDSYNGGAQNLSPSAYAQGAILGVLEPIYRSIQFCSSYSQYDALSCTLEIFSSSWLKKLDIITKEKVFSEIQLQKDVGLIAEWILAKKSLSDSLKRELLRQESLVELENRVTMLVSVQDKSKSEIGFLQRQTSEMGLKVAKFYEELCSRSNSPNDQVSPNISTKSNLSSFSSLLTKSGQAYFRSNKSDK 909          
BLAST of SMED30018898 vs. Planmine SMEST
Match: SMESG000006424.1 (SMESG000006424.1)

HSP 1 Score: 77.411 bits (189), Expect = 4.349e-14
Identity = 105/468 (22.44%), Postives = 195/468 (41.67%), Query Frame = 2
Query:  473 SRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVT-TGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKLTR----DLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFY--------------------QSPIVIDEIKLFRNKSRNQKSNRQLXXXXXXXXXXNRCLS-----------------SLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVIPIEQPSSCPFLNVKTCKPR---KGVQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLE---------IEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSILLNH 1714
            S +++   +  S+L  +R +CE +   L+    L S +           +++N L++++ ++ IL   +I  +  F+  TGL  LS SD+  Y+ ++LW  SRGIEEYN +  H     +  L R    D   ++  Y      +NK ID  +  ++T+S  K+   L+  RS ++A  +H + +                    +S I  D+I+L    S + +S+  L        +    L+                 S K   S + + +  E++F  + L+ +S+ST++L +L   +S  K   E                      N ++ + R   K VQW D  D  + + +I  YL+  W  V+  L   + SN   S S+          + K+GS  I N   +  +  ++K+    + FP+   P L +Q+  ++    + I +   C ++   H
Sbjct:  130 SVVHNGFESMKSQLSFIRNICEKMTFLLHQPSILRSKILKATNFGLSKERFQNLLKQFLRKF-ILHINVILFTSYFIVFTGLESLSFSDIKTYSDNELWNFSRGIEEYNKMFNHIKLIAQSSLFRTTMSDCRGSVFAY------LNKKID-PICLVKTISVRKVFKILSTFRSTIMAERIHRFLFCSKDVYKAIRHRYLNMILQPESSIYYDKIQLNSIDSESNRSDNSLNNTSRSLMEDIIHLNQEESNNKMKYKKFVQRLSTKMKHSSITEEVNSEFQFIVSLLENVSKSTDLLIKL--QISTEKFTAESR--------------------NKQSDESRIGKKNVQWRDSHDSKISNHMIKYYLDRYWYYVDTYLDMIE-SNLASSGSINPVRNQKISVVSKLGSHFIHNSQIKIALSNLMKEPLLTKNFPSELKPCLYNQSLKMQQSARMYICEAAFCKALGFGH 566          
BLAST of SMED30018898 vs. Planmine SMEST
Match: SMESG000006424.1 (SMESG000006424.1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.520e-14
Identity = 105/468 (22.44%), Postives = 195/468 (41.67%), Query Frame = 2
Query:  473 SRLYDSISTSDSRLECLRALCENLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQLSQEFVT-TGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKLTR----DLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIAHSVHNYFY--------------------QSPIVIDEIKLFRNKSRNQKSNRQLXXXXXXXXXXNRCLS-----------------SLKQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTVDNFMVPVIPIEQPSSCPFLNVKTCKPRKG---VQWSDHKDVSLRHQVIGQYLNVVWKLVNDLLKNTKYSNHYYSHSLE---------IEKMGSWLILNGSCRKFMGIIIKKIATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSILLNH 1714
            S +++   +  S+L  +R +CE +   L+    L S +           +++N L++++ ++ IL   +I  +  F+  TGL  LS SD+  Y+ ++LW  SRGIEEYN +  H     +  L R    D   ++  Y      +NK ID  +  ++T+S  K+   L+  RS ++A  +H + +                    +S I  D+I+L    S + +S+  L        +    L+                 S K   S + + +  E++F  + L+ +S+ST++L +L   +S  K   E                      N ++ + R G   VQW D  D  + + +I  YL+  W  V+  L   + SN   S S+          + K+GS  I N   +  +  ++K+    + FP+   P L +Q+  ++    + I +   C ++   H
Sbjct:  130 SVVHNGFESMKSQLSFIRNICEKMTFLLHQPSILRSKILKATNFGLSKERFQNLLKQFLRKF-ILHINVILFTSYFIVFTGLESLSFSDIKTYSDNELWNFSRGIEEYNKMFNHIKLIAQSSLFRTTMSDCRGSVFAY------LNKKID-PICLVKTISVRKVFKILSTFRSTIMAERIHRFLFCSKDVYKAIRHRYLNMILQPESSIYYDKIQLNSIDSESNRSDNSLNNTSRSLMEDIIHLNQEESNNKMKYKKFVQRLSTKMKHSSITEEVNSEFQFIVSLLENVSKSTDLLIKL--QISTEKFTAESR--------------------NKQSDESRIGKKNVQWRDSHDSKISNHMIKYYLDRYWYYVDTYLDMIE-SNLASSGSINPVRNQKISVVSKLGSHFIHNSQIKIALSNLMKEPLLTKNFPSELKPCLYNQSLKMQQSARMYICEAAFCKALGFGH 566          
The following BLAST results are available for this feature:
BLAST of SMED30018898 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V4A0827.996e-2825.58Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A0R3WD621.422e-2329.27Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
K1QA831.676e-2121.61Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A3S5ALJ31.687e-2129.87Uncharacterized protein (Fragment) OS=Protopolysto... [more]
A0A2T7PA972.448e-2122.56Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of SMED30018898 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018898 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000079137.10.000e+099.78SMESG000079137.1[more]
SMESG000006424.14.349e-1422.44SMESG000006424.1[more]
SMESG000006424.15.520e-1422.44SMESG000006424.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018898 ID=SMED30018898|Name=SMED30018898|organism=Schmidtea mediterranea sexual|type=transcript|length=3039bp
TTTCATTGACCTTGATTAATAAAAGATTTTATAAAAATAGTAATGGTGAT
AATGAGTTCTTAAAGAAAACAAATCAAATCTATTTAAAATGCATGCCAGT
TTAGGATTTATTGGTCGTGCTCAAACAATCGATTCTGATGAACTTCCAAT
GGATGATAATGCTGGTTCATTTTCTAAATCATCGACAGTTAATTCAGAGG
AACATAAGTCAATTATATCATCGATTCACAGTAAATTCATATCTCTGAAT
TATTTCTCTCATTCTTTTCTCCGCTTCAAATATGACAAAATGGATTTTCA
TTGCAATCACAAGCTATCAATACAAACTCATATTAATGATCTAATTCAAA
ATTACCGAAAAATTCAAGAGTTGGAATTATTCAGCAATATCTTATCTCGA
CTTCAAGAATTTTATAACCTTATTGAATACATTAAAAGTCTCATAATTCA
TGAACACCAAAATCTAAAATTGTCCCGATTATATGATTCAATTTCAACAT
CAGATTCTCGGTTGGAGTGCTTGAGAGCTTTATGTGAAAATCTAAGAATT
TGTTTAAATAATACTTGGTCACTTTGTAGTTATGTACAATGCAACAAGAC
TCTCCAATCGTATTTATTCAAATACCGTAATTTTCTCGAAAAATATATGG
ATAGATATCAAATTTTAACGACCAAACTTATTCAACTGAGTCAAGAATTC
GTTACAACTGGACTTTTAGTCCTATCACATTCAGATTTGCTTCGTTACAC
TCATGATGATTTATGGGAGATTTCTAGAGGCATTGAAGAATACAACATTA
TAATTGAACACAGTTTATACCAAAAACGTTTCAAATTGACCCGAGACTTA
TATATGAATATAAATCATTATGAGGTCACTTTGAATTCAGTCAACAAAAG
TATCGACCAACAACTTTTCGGAATTCGAACATTGTCTTTCAGCAAAATCC
TTTACAAACTTGCTTTAGAACGGTCACTTCTGATCGCCCACAGTGTACAT
AATTATTTCTATCAGTCCCCGATTGTCATCGATGAAATCAAATTATTTCG
TAATAAAAGTCGAAATCAAAAATCAAATCGACAATTATTTGAATTTCAAG
AAGACGATTTCGATTTTAATCGCTGTCTTTCATCTTTAAAACAAATCGGA
TCTCCTGTCAATGATTATCTGAGATTGGAGTATGAGTTCACGTCTAATTT
TCTCGATATTATTTCACAGTCGACGGAAATGTTGAAGGAATTGAGTAATT
CAGTTTCAAAGCGAAAACTCTGTAAAGAAGATACAGTTGATAATTTCATG
GTTCCTGTGATTCCTATCGAACAACCTAGCAGTTGTCCATTTCTCAACGT
GAAAACGTGCAAACCCAGAAAAGGTGTTCAATGGAGTGATCACAAGGATG
TGAGTTTGCGGCATCAAGTGATTGGCCAGTATTTGAACGTAGTTTGGAAA
TTGGTAAATGACTTATTAAAAAATACCAAATATTCAAATCATTATTATTC
CCATTCTTTGGAAATTGAAAAAATGGGATCTTGGCTAATATTAAATGGTA
GTTGTAGAAAATTTATGGGAATTATCATCAAAAAAATTGCCACTCCTGAG
ATTTTCCCCACTGGCTTCGTTCCAATTCTTCTACATCAAGCAGAGCTGAT
TGAATTCGAAAATTTGTTGCTCATATGGGATCAAGGGATGTGCACCTCAA
TACTACTCAACCACCATATGCATTCTCCTGACCAAATTCTTGCTGATAAT
ATGTCAGAAATATCAACAAAAATTTATCAACATCTCCAATCTCAAACGAA
TGAATTCAACAATAATAATTACAAAGAAAAACATGTGGTAAAACGCGAAA
ATGAGCACTTGGAACAAGTCGACCCAACAGTGACGATCATTTCGGTTTTG
ACGAGCACATTTCAGAAATACGGAGAAAAATTATTGTCTGTATCAAAATC
AGCTTCAAATCGCTTGAATTCTGGAGAAATCGCACAATTAATATGGCCAA
CCATCGCAAATTGGGCTCATTTGGAAATGCAATTTTATTCATTCCTGCAG
TCATATGGGTATTTTGGGTCCGACATAAATACATCCACAAGCGATAAATT
AAGATTGATATCCAGAAATATTCCTGGTCTATCGTTAGACAATAAAATAA
GCAATCTGAAAAAATCTCTCTTGGAATATTACACAATAAAATGCAGTGAA
CGCGTCAACCAATTATTAGATCTTGGACTTATGCAGAAATGTACAAAATT
TGACTCATATAATGGAGGTGCTCAAAATCTTTCTCCATCTGCATATGCAC
AAGGCGCTATTCTTGGAGTTTTAGAACCAATTTACAGATCCATTCAGTTT
TGTTCAAGTTACTCACAGTATGATGCTTTATCGTGTACACTTGAAATATT
TTCCTCTTCTTGGCTAAAAAAGCTTGATATCATAACCAAAGAAAAAGTTT
TTTCTGAAATTCAACTGCAAAAAGATGTTGGTCTTATTGCCGAATGGATT
TTGGCCAAAAAATCGCTTTCTGATTCTCTAAAAAGAGAATTACTTCGTCA
AGAGTCTCTAGTTGAATTGGAAAATCGAGTCACAATGTTGGTTTCTGTTC
AGGATAAATCAAAATCGGAAATTGGTTTTCTTCAACGTCAGACATCAGAA
ATGGGTTTGAAGGTAGCAAAATTTTATGAAGAGCTTTGCTCAAGATCCAA
CAGTCCAAACGATCAAGTTTCTCCAAATATTTCAACAAAATCCAATTTAT
CATCCTTTAGCAGTCTGCTCACAAAAAGCGGGCAGGCATATTTCCGCTCA
AATAAATCTGATAAATAATTATGCAATAGTGTCTCCCGGTTTTTTAGTGA
TTTACAATTATTTTTGGTGTTAAATTAGCCTAGATAAATTTACCATTAGT
TCTGGTTTGGCAAAGTGACATGAATATTTTTCTGTGAAAATGTGAACATT
TTGAAACAAAAAGACCAATTTTGTTTAACAATATATAGTTAGATGTATTG
TGTATATTACAATATTTATCATTTTTGAAATAAATCAAC
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protein sequence of SMED30018898-orf-1

>SMED30018898-orf-1 ID=SMED30018898-orf-1|Name=SMED30018898-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=910bp
MHASLGFIGRAQTIDSDELPMDDNAGSFSKSSTVNSEEHKSIISSIHSKF
ISLNYFSHSFLRFKYDKMDFHCNHKLSIQTHINDLIQNYRKIQELELFSN
ILSRLQEFYNLIEYIKSLIIHEHQNLKLSRLYDSISTSDSRLECLRALCE
NLRICLNNTWSLCSYVQCNKTLQSYLFKYRNFLEKYMDRYQILTTKLIQL
SQEFVTTGLLVLSHSDLLRYTHDDLWEISRGIEEYNIIIEHSLYQKRFKL
TRDLYMNINHYEVTLNSVNKSIDQQLFGIRTLSFSKILYKLALERSLLIA
HSVHNYFYQSPIVIDEIKLFRNKSRNQKSNRQLFEFQEDDFDFNRCLSSL
KQIGSPVNDYLRLEYEFTSNFLDIISQSTEMLKELSNSVSKRKLCKEDTV
DNFMVPVIPIEQPSSCPFLNVKTCKPRKGVQWSDHKDVSLRHQVIGQYLN
VVWKLVNDLLKNTKYSNHYYSHSLEIEKMGSWLILNGSCRKFMGIIIKKI
ATPEIFPTGFVPILLHQAELIEFENLLLIWDQGMCTSILLNHHMHSPDQI
LADNMSEISTKIYQHLQSQTNEFNNNNYKEKHVVKRENEHLEQVDPTVTI
ISVLTSTFQKYGEKLLSVSKSASNRLNSGEIAQLIWPTIANWAHLEMQFY
SFLQSYGYFGSDINTSTSDKLRLISRNIPGLSLDNKISNLKKSLLEYYTI
KCSERVNQLLDLGLMQKCTKFDSYNGGAQNLSPSAYAQGAILGVLEPIYR
SIQFCSSYSQYDALSCTLEIFSSSWLKKLDIITKEKVFSEIQLQKDVGLI
AEWILAKKSLSDSLKRELLRQESLVELENRVTMLVSVQDKSKSEIGFLQR
QTSEMGLKVAKFYEELCSRSNSPNDQVSPNISTKSNLSSFSSLLTKSGQA
YFRSNKSDK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR026700CCDC142
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
IPR026700Coiled-coil domain-containing protein 142PFAMPF14923CCDC142coord: 732..894
e-value: 1.4E-13
score: 50.5
IPR026700Coiled-coil domain-containing protein 142PANTHERPTHR21436UNCHARACTERIZEDcoord: 732..894
coord: 69..588