Store-operated calcium entry-associated regulatory factor

Overview
NameStore-operated calcium entry-associated regulatory factor
Smed IDSMED30018884
Length (bp)1012
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Store-operated calcium entry-associated regulatory factor (SMED30018884) t-SNE clustered cells

Violin plots show distribution of expression levels for Store-operated calcium entry-associated regulatory factor (SMED30018884) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Store-operated calcium entry-associated regulatory factor (SMED30018884) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Store-operated calcium entry-associated regulatory factor (SMED30018884) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018884

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30018884SMESG000037963.1 dd_Smed_v4_809_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30018884SMESG000037963.1 dd_Smed_v4_809_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Human
Match: SARAF (store-operated calcium entry associated regulatory factor [Source:HGNC Symbol;Acc:HGNC:28789])

HSP 1 Score: 147.517 bits (371), Expect = 5.668e-41
Identity = 105/308 (34.09%), Postives = 156/308 (50.65%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX----------------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPE--YESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSR---TNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSD------SDETQEYRTASGYGGTSRR 914
            +++ L D++ LTL +   TT RR  P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    +  +                 +++ +  + +GL  +V  + +  ++Y   L+         S     +  +   T    PPPPGF+ ++T P    + +       F+       +GPGFWTGLG GG++GYLFGS    T    +++ P+   +    W   YS  P H         S+   + RTASGYGGT RR
Sbjct:   35 DRMLLRDVKALTLHYDRYTTSRRLDPIPQLKCVGGTAGC-DSYTPKVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSDYYYKWSSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFTNSAGPPPPGFKSEFTGPQNTGHGATSGFGSAFTGQQGYENSGPGFWTGLGTGGILGYLFGSNRAATPFSDSWYYPSYPPSYPGTWNRAYS--PLHGGSGSYSVCSNSDTKTRTASGYGGTRRR 339
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Human
Match: SARAF (store-operated calcium entry associated regulatory factor [Source:HGNC Symbol;Acc:HGNC:28789])

HSP 1 Score: 137.887 bits (346), Expect = 3.370e-37
Identity = 86/246 (34.96%), Postives = 130/246 (52.85%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX----------------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPE--YESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGS 755
            +++ L D++ LTL +   TT RR  P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    +  +                 +++ +  + +GL  +V  + +  ++Y   L+         S     +  +   T    PPPPGF+ ++T P    + +       F+       +GPGFWTGLG GG++GYLFGS
Sbjct:   35 DRMLLRDVKALTLHYDRYTTSRRLDPIPQLKCVGGTAGC-DSYTPKVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSDYYYKWSSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFTNSAGPPPPGFKSEFTGPQNTGHGATSGFGSAFTGQQGYENSGPGFWTGLGTGGILGYLFGS 279
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Human
Match: SARAF (store-operated calcium entry associated regulatory factor [Source:HGNC Symbol;Acc:HGNC:28789])

HSP 1 Score: 125.946 bits (315), Expect = 3.526e-34
Identity = 68/198 (34.34%), Postives = 103/198 (52.02%), Query Frame = 3 
Query:   96 LTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX----------------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAP 629
            L D++ LTL +   TT RR  P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    +  +                 +++ +  + +GL  +V  + +  ++Y   L+         S     +  +   T    PPPPGF+ ++T P
Sbjct:    3 LRDVKALTLHYDRYTTSRRLDPIPQLKCVGGTAGC-DSYTPKVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSDYYYKWSSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFTNSAGPPPPGFKSEFTGP 199
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Human
Match: SARAF (store-operated calcium entry associated regulatory factor [Source:HGNC Symbol;Acc:HGNC:28789])

HSP 1 Score: 110.538 bits (275), Expect = 5.854e-29
Identity = 51/95 (53.68%), Postives = 64/95 (67.37%), Query Frame = 3 
Query:  111 VLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLD 395
             LTL +   TT RR  P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD
Sbjct:   35 ALTLHYDRYTTSRRLDPIPQLKCVGGTAGC-DSYTPKVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLD 128
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Human
Match: SARAF (store-operated calcium entry associated regulatory factor [Source:HGNC Symbol;Acc:HGNC:28789])

HSP 1 Score: 70.8626 bits (172), Expect = 8.706e-14
Identity = 72/229 (31.44%), Postives = 104/229 (45.41%), Query Frame = 3 
Query:  300 KFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX----------------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNE----NGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            KFG   V+CEGY+   D+YV +GSCGL Y LD +    +  +                 +++ +  + +GL  +V  + +  ++Y   L+         S     +  +   T    PPPPGF+ ++T P    + +     F S   G +    +GPGFWTGLG GG++GYLFGS             YS  H       S      S+SD   + RTASGYGGT RR
Sbjct:    4 KFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSDYYYKWSSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFTNSAGPPPPGFKSEFTGPQ--NTGHGATSGFGSAFTGQQGYENSGPGFWTGLGTGGILGYLFGSN----------RAYSPLHGG-----SGSYSVCSNSD--TKTRTASGYGGTRRR 213
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Celegans
Match: T06D8.9 (pep chromosome:WBcel235:II:11239407:11240988:1 gene:WBGene00011529.1 transcript:T06D8.9.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T06D8.9)

HSP 1 Score: 134.42 bits (337), Expect = 1.459e-36
Identity = 103/312 (33.01%), Postives = 146/312 (46.79%), Query Frame = 3 
Query:   57 LFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXXYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQN-------------------------ASMPSTSNFGFADETPPPP-GFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            +FF ++ A +K+ L D+  +TL  G +TTGRR  P  QL+CVGGSA+    + P+ VQC N G DG  VQW+C ADL + ++FGS+ V+CEGYDY  D Y+ +GSCGL Y L+ +S S        +++   W+   F  F +V    I Y   A  T   QN                              ++   + D + PPP GFR D  +    +         S  + G      FWTG   G + GYL  S  N   A+ +P      +  + +D          S  T   R++SGYGGT+RR
Sbjct:   20 VFFETSAASDKVLLRDVSAITLHKGKMTTGRRVSPTFQLKCVGGSAK--GAFTPKVVQCANQGFDGSDVQWRCDADLPHDMEFGSISVSCEGYDYAEDPYILRGSCGLEYELEYNSASGNRSVSRKSSQ-DRWD--QFATFVVVA--FIAYIIYAMWTNRNQNPESSGYTSGGSGGPGGPGSGGGGGGPGGYPSAPPPYDDYSKPPPYGFRGDSQSGGGCQGSSSGGASGSGANNGGS----FWTGASLGAIGGYLASSFLNNNNAYARPR----YNRGFFQDTGFSSSDSWSSPSTSSMRSSSGYGGTTRR 316
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Celegans
Match: T06D8.9 (pep chromosome:WBcel235:II:11239718:11240988:1 gene:WBGene00011529.1 transcript:T06D8.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T06D8.9)

HSP 1 Score: 134.42 bits (337), Expect = 1.459e-36
Identity = 103/312 (33.01%), Postives = 146/312 (46.79%), Query Frame = 3 
Query:   57 LFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXXYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQN-------------------------ASMPSTSNFGFADETPPPP-GFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            +FF ++ A +K+ L D+  +TL  G +TTGRR  P  QL+CVGGSA+    + P+ VQC N G DG  VQW+C ADL + ++FGS+ V+CEGYDY  D Y+ +GSCGL Y L+ +S S        +++   W+   F  F +V    I Y   A  T   QN                              ++   + D + PPP GFR D  +    +         S  + G      FWTG   G + GYL  S  N   A+ +P      +  + +D          S  T   R++SGYGGT+RR
Sbjct:   20 VFFETSAASDKVLLRDVSAITLHKGKMTTGRRVSPTFQLKCVGGSAK--GAFTPKVVQCANQGFDGSDVQWRCDADLPHDMEFGSISVSCEGYDYAEDPYILRGSCGLEYELEYNSASGNRSVSRKSSQ-DRWD--QFATFVVVA--FIAYIIYAMWTNRNQNPESSGYTSGGSGGPGGPGSGGGGGGPGGYPSAPPPYDDYSKPPPYGFRGDSQSGGGCQGSSSGGASGSGANNGGS----FWTGASLGAIGGYLASSFLNNNNAYARPR----YNRGFFQDTGFSSSDSWSSPSTSSMRSSSGYGGTTRR 316
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Zebrafish
Match: saraf (store-operated calcium entry-associated regulatory factor [Source:NCBI gene;Acc:767649])

HSP 1 Score: 134.806 bits (338), Expect = 2.864e-36
Identity = 61/104 (58.65%), Postives = 75/104 (72.12%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLD 395
            E + L D+QVLTL  G  TT RRS PVPQL+C+GGSA C   + P  VQC N GSDG   QW+C+AD+ N  +FG V+V+CEGY+ PND YV +GSCGL YTL+
Sbjct:   25 EAVLLRDVQVLTLYRGRYTTARRSSPVPQLQCIGGSAGC-GSFTPEVVQCYNRGSDGIDAQWECKADMDNLYRFGRVEVSCEGYNSPNDAYVLRGSCGLEYTLE 127
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Zebrafish
Match: saraf (store-operated calcium entry-associated regulatory factor [Source:NCBI gene;Acc:767649])

HSP 1 Score: 134.806 bits (338), Expect = 3.082e-36
Identity = 61/104 (58.65%), Postives = 75/104 (72.12%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLD 395
            E + L D+QVLTL  G  TT RRS PVPQL+C+GGSA C   + P  VQC N GSDG   QW+C+AD+ N  +FG V+V+CEGY+ PND YV +GSCGL YTL+
Sbjct:   25 EAVLLRDVQVLTLYRGRYTTARRSSPVPQLQCIGGSAGC-GSFTPEVVQCYNRGSDGIDAQWECKADMDNLYRFGRVEVSCEGYNSPNDAYVLRGSCGLEYTLE 127
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Xenopus
Match: SARAF (store-operated calcium entry-associated regulatory factor [Source:NCBI gene;Acc:100496655])

HSP 1 Score: 154.836 bits (390), Expect = 5.023e-44
Identity = 116/298 (38.93%), Postives = 159/298 (53.36%), Query Frame = 3 
Query:   81 HEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXX----------YTATEKSSW----NGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPH-TAWREDYSAQPRHFS-DSDETQEYRTASGYGGTSRR 914
             E++ L DIQ +TL     T  RRS PVPQL+C+GGSA C    +P+ VQC N G DGF VQW+C+ D+ N  +FG V+V+CEG+DYP D YV +GSCGL YTL+ +    K  Q           Y  +  +SW    NG   +V  ++V +    + L  +    Q    P    + F  +T    PPPPGF+ D+T    + S Y       ++H+    GPGFWTGLG GG++GYLFG+R  Q         Y AP+   W    ++   H +    ++   RTASG+GGT RR
Sbjct:   30 QERVLLRDIQAITLYADRYTNARRSAPVPQLKCIGGSAGCH-TMVPQVVQCHNRGWDGFDVQWECKVDMDNSYRFGKVEVSCEGFDYPEDPYVLRGSCGLEYTLELTEEGRKRSQDGYRSGGFGSGYFQSNSNSWDTKTNGSSAIVLIVIVIIAYGVYKLFLSGPSVQEHPRPDEYGYDFQSQTRASAPPPPGFKSDFTG---FSSGY------GTSHS--NQGPGFWTGLGTGGVLGYLFGNRRAQP--------YQAPYFNTWTGPSNSDSMHQNYRPPQSSGVRTASGFGGTKRR 307
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Mouse
Match: Saraf (store-operated calcium entry-associated regulatory factor [Source:MGI Symbol;Acc:MGI:1915137])

HSP 1 Score: 134.806 bits (338), Expect = 3.939e-36
Identity = 110/307 (35.83%), Postives = 153/307 (49.84%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXX----------YTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET--------PPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGN---ENGPGFWTGLGAGGLMGYLFGSR---TNQQAAFHQPTGYSAPHTAWREDYSAQP------RHFSDSDETQEYRTASGYGGTSRR 914
            ++I L D++ LTL     TT RR  P+PQL+CVGG+A CE  Y PR +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    K  +           Y     S   G   +    V+   +Y   L+      Q +  P + +  +++ +         PPPGF+ ++T P    + Y     F S   G     +GPGFW+GLGAGGL+GYLFGS    T    +++ P  Y   H+      +  P       + + S+     RTASGYGGT RR
Sbjct:   65 DRILLRDVKALTLYSDRYTTSRRLDPIPQLKCVGGTAGCE-AYTPRVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLKKLKESGKHQGFSDYYHKLYSSDSCGFITIAVLFVLAFAVYKLFLSDG----QGSPPPYSEHPPYSEHSQRFASAAGAPPPGFKSEFTGP--QNTGYGASSGFGSAFGGQGYGSSGPGFWSGLGAGGLLGYLFGSNRAATPFSDSWYHPA-YPPSHSGAWNSRAYSPLGGGAGSYCASSNADSRTRTASGYGGTRRR 363
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. UniProt/SwissProt
Match: sp|F7C1E2|SARAF_XENTR (Store-operated calcium entry-associated regulatory factor OS=Xenopus tropicalis OX=8364 GN=saraf PE=3 SV=2)

HSP 1 Score: 160.614 bits (405), Expect = 1.699e-45
Identity = 118/300 (39.33%), Postives = 162/300 (54.00%), Query Frame = 3 
Query:   81 HEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXX----------YTATEKSSW----NGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPEYESIYRNKRPFSSTH--AGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPH-TAWREDYSAQPRHFS-DSDETQEYRTASGYGGTSRR 914
             E++ L DIQ +TL     T  RRS PVPQL+C+GGSA C    +P+ VQC N G DGF VQW+C+ D+ N  +FG V+V+CEG+DYP D YV +GSCGL YTL+ +    K  Q           Y  +  +SW    NG   +V  ++V +    + L  +    Q    P    + F  +T    PPPPGF+ D+T  P    I+   + FSS +  + +  GPGFWTGLG GG++GYLFG+R  Q         Y AP+   W    ++   H +    ++   RTASG+GGT RR
Sbjct:   30 QERVLLRDIQAITLYADRYTNARRSAPVPQLKCIGGSAGCH-TMVPQVVQCHNRGWDGFDVQWECKVDMDNSYRFGKVEVSCEGFDYPEDPYVLRGSCGLEYTLELTEEGRKRSQDGYRSGGFGSGYFQSNSNSWDTKTNGSSAIVLIVIVIIAYGVYKLFLSGPSVQEHPRPDEYGYDFQSQTRASAPPPPGFKSDFTD-PHLCKIFGASKSFSSGYGTSHSNQGPGFWTGLGTGGVLGYLFGNRRAQP--------YQAPYFNTWTGPSNSDSMHQNYRPPQSSGVRTASGFGGTKRR 319
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. UniProt/SwissProt
Match: sp|Q0IHF9|SARAF_XENLA (Store-operated calcium entry-associated regulatory factor OS=Xenopus laevis OX=8355 GN=saraf PE=2 SV=2)

HSP 1 Score: 156.762 bits (395), Expect = 4.322e-44
Identity = 112/299 (37.46%), Postives = 155/299 (51.84%), Query Frame = 3 
Query:   81 HEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXX----------YTATEKSSW----NGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET---PPPPGFRPDYTAPPEYESIYRNKRPFSSTHA-GNEN-GPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPH-TAWREDYSAQPRHFSDS-DETQEYRTASGYGGTSRR 914
             +++ L DIQ +TL     T  RRS PVPQL+C+GG+A C    +P+ VQC N G DG  VQW+C+ D+ N  +FG V+V+CEG+D+P D YV +GSCGL YTL+ +    K  Q           Y  +   SW    +G   +V   +V L    + L  +    Q    P    +    +T    PPPGF+ D+T              FSS +  G+ N GPGFWTGLG GG++GYLFG+R  Q         Y +P+   W     ++  H +D   ++   RTASG+GGT RR
Sbjct:   27 QDRVLLRDIQTITLYADRYTNARRSAPVPQLKCIGGNAGCH-AMVPQVVQCHNRGWDGLDVQWECRVDMDNSYRFGKVEVSCEGFDFPEDPYVLRGSCGLEYTLELTEEGRKRTQDGYRSAGFGSGYFQSNSQSWDSKTDGSSAIVLIFIVILAYGVYKLFLSGPSVQQHPRPDEYGYDHQSQTHSSAPPPGFKSDFTG-------------FSSGYGTGHVNQGPGFWTGLGTGGVLGYLFGNRRAQP--------YQSPYFNTWTGPSHSESMHQNDRPPQSSGVRTASGFGGTKRR 303
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. UniProt/SwissProt
Match: sp|Q96BY9|SARAF_HUMAN (Store-operated calcium entry-associated regulatory factor OS=Homo sapiens OX=9606 GN=SARAF PE=1 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 2.722e-40
Identity = 105/308 (34.09%), Postives = 156/308 (50.65%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX----------------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPE--YESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSR---TNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSD------SDETQEYRTASGYGGTSRR 914
            +++ L D++ LTL +   TT RR  P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    +  +                 +++ +  + +GL  +V  + +  ++Y   L+         S     +  +   T    PPPPGF+ ++T P    + +       F+       +GPGFWTGLG GG++GYLFGS    T    +++ P+   +    W   YS  P H         S+   + RTASGYGGT RR
Sbjct:   35 DRMLLRDVKALTLHYDRYTTSRRLDPIPQLKCVGGTAGC-DSYTPKVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSDYYYKWSSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFTNSAGPPPPGFKSEFTGPQNTGHGATSGFGSAFTGQQGYENSGPGFWTGLGTGGILGYLFGSNRAATPFSDSWYYPSYPPSYPGTWNRAYS--PLHGGSGSYSVCSNSDTKTRTASGYGGTRRR 339
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. UniProt/SwissProt
Match: sp|Q5R491|SARAF_PONAB (Store-operated calcium entry-associated regulatory factor OS=Pongo abelii OX=9601 GN=SARAF PE=2 SV=2)

HSP 1 Score: 145.206 bits (365), Expect = 2.222e-39
Identity = 104/306 (33.99%), Postives = 154/306 (50.33%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX----------------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADET----PPPPGFRPDYTAPPE--YESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSR---TNQQAAFHQPTGYSAPHTAWREDYS----AQPRHFSDSDETQEYRTASGYGGTSRR 914
            E + L D++ LTL +   TT RR  P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    +  +                 + + +  + +GL  +V  + +  ++Y   L+         S     +  +   T    PPPPGF+ ++T P    + +       F+       +GPGFWTGLG GG++GYLFGS    T    +++ P+   +    W   YS        + + S+   + RTASGYGGT RR
Sbjct:   35 ECMLLRDVKALTLHYDRYTTSRRLDPIPQLKCVGGTAGC-DSYTPKVIQCQNKGWDGYDVQWECKTDLDIAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSDYYYKWYSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFTNSAGPPPPGFKSEFTGPQNTGHGATSGFGSAFTGQQGYENSGPGFWTGLGTGGILGYLFGSNRAATPFSDSWYYPSYPPSYPGTWNRAYSPLRGGSGSYSACSNSDTKTRTASGYGGTRRR 339
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. UniProt/SwissProt
Match: sp|Q08E24|SARAF_BOVIN (Store-operated calcium entry-associated regulatory factor OS=Bos taurus OX=9913 GN=SARAF PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.827e-39
Identity = 109/318 (34.28%), Postives = 156/318 (49.06%), Query Frame = 3 
Query:   81 HEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXX----------------XXYTATEKSSWNGLYFVVFCIVVCLMIYYFCLA-------------PNTTGFQNASMPSTSNFGFADETPPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSD-----------SDETQEYRTASGYGGTSRR 914
             ++I L DI+ LTL +   TT RR +P+PQL+CVGG+A C D Y P+ +QC N G DG+ VQW+C+ DL    KFG   V+CEGY+   D+YV +GSCGL Y LD +    K                      + E SS  G+  +V  + +   +Y F L+             P +  +Q  S         +   PPP GF+ ++T P    + + +   F+       +GPGFWTGLG GG++GYLFGS  N+ A     + Y +   ++   +++  R +S            S      RTASGYGGT RR
Sbjct:   34 RDRILLRDIKALTLYYDRYTTSRRLEPIPQLKCVGGTAGC-DSYTPKVIQCQNRGWDGYDVQWECKTDLDVAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYQLDYTELGLKKLRESGKEHGFNSFSNYYSKLYSPESSSLGGVVTIVVLLAIAFGVYKFFLSDGHESPPPYSEDPPYSHRYQRFS---------SSAGPPPAGFKSEFTGPHGATAGFGSA--FTGQQGHEHSGPGFWTGLGTGGILGYLFGS--NRAATPFSDSWYPSYPPSYTSTWNS--RAYSPLRGGPGSSSACSGSEPRTRTASGYGGTRRR 335
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. TrEMBL
Match: A0A2C9KD54 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106062936 PE=4 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.429e-56
Identity = 117/302 (38.74%), Postives = 154/302 (50.99%), Query Frame = 3 
Query:   63 FSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX-------XYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADETPPPPGFRPDYT----APPEYESIYRNKRPFSST-------HAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            F    + +KI L+D++ LTL  G +T  RRS PVPQL+CVGGSAQ   K+ P+TVQC N GSDG+ +QW+C+ D+ N  +FG V+V CEGYDYPND Y+ KGSCGL YT++ +    +           YT   +   +GL+  +  ++V  +IY  CLAPN                   E PPP GFR +Y      PP Y+        F +T       HA   NG GFWTG   GGL+GYLFG   N    +   T   +          A     S    +   R  SG+GGT+RR
Sbjct:   29 FGDYHSSDKILLSDVKALTLHQGRMTNSRRSSPVPQLKCVGGSAQY--KFTPKTVQCINRGSDGYDIQWECKTDMDNAYRFGHVEVTCEGYDYPNDPYILKGSCGLEYTVELTEEGRQKESYRGGSPYSYTYKSEGGISGLFVFIGILLVMYIIYKLCLAPNVVTH--------------GEQPPPYGFRQEYMPSQGPPPPYDGYSSASFGFGNTYPKPGYPHASATNGGGFWTGAATGGLLGYLFGRGNNYNTGWGTGTWGGSGFNHPTSSGGAFFSSSSGGPSSSGSRMTSGFGGTTRR 314
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. TrEMBL
Match: A0A3S1CCW5 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_003008 PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 1.296e-54
Identity = 124/329 (37.69%), Postives = 173/329 (52.58%), Query Frame = 3 
Query:   21 LFMDAINYFIAFLFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXX-------XYTATEKSS----WNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADETPPPPGFRPDYTA----PPEYE----------SIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWRED------YSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            L + A+  F A   F+     +K+ L+D++ LTL+ G +T  RRS PVPQL+CVGG+AQ   K+ P+ VQC NMGSDG  +QW+C+ D+ N  +FG+V+V CEGYDYPND YV KGSCGL YTL+ +    +  +        Y+   K S     +GL+     ++   +IY  C+AP   G              AD+ PPP GFRP++T     PP Y+            + +       H+    G GFW+G   GGL+GY+FGSR N         G+S    +W+        +S+     S S  +   RTASG+GGTSRR
Sbjct:   23 LILTAVLTF-ATAAFNDYHHSDKMLLSDVKALTLRQGQMTNARRSPPVPQLKCVGGAAQY--KFTPKVVQCINMGSDGHDIQWECKTDMDNAYRFGNVEVTCEGYDYPNDPYVLKGSCGLEYTLEFTEEGVQRERQKGGSGYQYSYNSKHSSEGGMSGLFVFAAVLLAIYVIYKTCIAPTAQGAYQTG---------ADQQPPPYGFRPEFTQPQGPPPPYDGWGTPDPSCGQSHYSAGYGRQHHSAGGTGGGFWSGAATGGLLGYMFGSRNN--------AGHSYRGRSWQSAAPSGGFFSSDSGSPSSSRGSSGTRTASGFGGTSRR 331
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. TrEMBL
Match: A0A1D2N1N0 (Store-operated calcium entry-associated regulatory factor OS=Orchesella cincta OX=48709 GN=Ocin01_07739 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 3.223e-52
Identity = 113/267 (42.32%), Postives = 148/267 (55.43%), Query Frame = 3 
Query:   78 AHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLD-XXXXXXXXXXXYTATEKSSWNGLYF-------VVFCIVVC--------LMIYYFCLAPNTTGFQ----NASMPSTSNFGFADETPPPPGFRPDYTAPPE------YESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQ---QAAFHQPT 791
             ++K+KL D+QVLTL  G +TTGRRS PVPQL CVGGSA     ++P+TVQC N GSDG+ VQW+C+ D+ N  KFG + V CEGYDYP+D YV KGSCGL YTLD + S +++  Q +  +    WN   +            + C          IY  C+ PN    Q    N   P     G    +PPPPGFR DY    +       +S + N +  SS      N  GFWTG  AGGL+GY+FG+R N    Q A+++P+
Sbjct:   26 GNDKVKLNDVQVLTLYTGAMTTGRRSSPVPQLRCVGGSA--NGAFVPQTVQCYNKGSDGYDVQWECKTDMDNAYKFGKISVTCEGYDYPDDPYVLKGSCGLEYTLDYTGSGNAQHSQQHGGSSDYGWNSNVYPKAQKQGDFVTNLACGAVVVVIVYAIYKTCITPNEQTRQRSNTNDDYPRRPGPGTGSSSPPPPGFRSDYGTGNDGGYASGGDSCHANSQGSSSGSGMGRNAGGFWTGAAAGGLLGYMFGNRNNNATYQGAYYRPS 290
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. TrEMBL
Match: A0A0L8GAG0 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22036897mg PE=4 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 6.560e-52
Identity = 116/289 (40.14%), Postives = 161/289 (55.71%), Query Frame = 3 
Query:   75 LAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXX--XXYTATEKS--SWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADETPPPP-GFRPDY----TAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            +   K+ L D+ V+TL HG +TT +RS PVPQL+C GGSA C   ++P+TVQC N G DG+ +QW+C+ D+  K +FG + V+CEGYDY +D Y+  GSCGL Y +D  +  S      HY++  +S   +  ++  +F I++   +Y  C A  T     +S P  +  G     PPPP GFR DY    +APP Y          SSTH+ N   PGF++GL +G L+GYLFG+R    +  +   GY          Y + P     S ET   RTASG+GGT RR
Sbjct:   21 IEGRKVLLQDVNVITLNHGQMTTAKRSSPVPQLKCTGGSAGCR-SFIPQTVQCYNRGWDGYDIQWECKTDMDKKYRFGQIVVSCEGYDYADDPYILAGSCGLEYDIDYVNQKSSGSYFNHYSSKRESGLGFGSIFTFLFVIMIIFFVYRTCFATPT---DPSSFPGGAQSGPTPSGPPPPYGFRSDYCSGQSAPPPYS---------SSTHSNNGQQPGFFSGLASGALLGYLFGNRGGYHSNSYYSDGYQRDWFGSPTGYHSGPTSSFSSSET---RTASGFGGTKRR 293
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. TrEMBL
Match: A0A218UMI4 (Store-operated calcium entry-associated regulatory factor OS=Lonchura striata domestica OX=299123 GN=SARAF PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.649e-50
Identity = 106/291 (36.43%), Postives = 154/291 (52.92%), Query Frame = 3 
Query:   87 KIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXX-------XXYTATEKSSWN---GLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSN-FGFADETPPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRH----FSDSDETQEYRTASGYGGTSRR 914
            K+ L D+Q LTL  G  TT RR+  +PQL+C GGSA C   + P  VQC N G DG+ VQW+C+ADL N  +FG ++V+CEGYDYP+D Y+ +GSC L + L+ ++   +           Y  + K   +   G   V+  +V+   +Y F L+   +   +      S  F  + + PPPPGF+  +T    +          + +H G  +GPGFWTGLGAG L+GYL G+        H+   +S+ H+ W E  +  P H     S    +   RTA+G+GGT RR
Sbjct:   27 KVLLRDVQALTLHRGQYTTSRRTASIPQLQCTGGSAGCS--HAPEVVQCYNKGWDGYDVQWQCKADLENAYRFGQMEVSCEGYDYPDDPYILRGSCSLLFKLELTAEGERKMKNSGNFGSSYYQSRKDYSDFGSGAIVVIVLLVLAFGVYKFFLSSQQSQQSSGGNDGFSRPFWQSQQAPPPPGFKSTFTDDNSFG---------THSHHGTSSGPGFWTGLGAGSLLGYLAGN--------HRAQPHSSYHSMWTETTAVPPMHGYSRNSIKGSSSGTRTATGFGGTKRR 298
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Cavefish
Match: saraf (store-operated calcium entry-associated regulatory factor [Source:NCBI gene;Acc:103026500])

HSP 1 Score: 129.413 bits (324), Expect = 2.356e-34
Identity = 74/212 (34.91%), Postives = 109/212 (51.42%), Query Frame = 3 
Query:   90 IKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXX-----------------------------XXYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNT---TGFQNASMPSTSNFG-FADETPPPPGFRPDYTA 626
            + L D+QVLTL  G  T  RR+ P+PQL+CVGG+A C   ++P  VQC N G DG  VQW+C+AD+ N  +FG V+V+CEG++ P+D Y+ +GSCGL YTL+ ++   +                                    +      GL  +   +++   +Y   L   T    GFQ     + +  G +++  PPPPGF+PDYTA
Sbjct:   26 VLLRDVQVLTLYKGRYTAARRTSPIPQLQCVGGTAGC-GSFVPEVVQCQNKGWDGVDVQWECKADMDNWYRFGRVEVSCEGFNSPDDAYILQGSCGLEYTLELTAEGKQHQGSRGSSGSSGFAGFASSFFQDKGHSGGGYYQQGGSSGEGAGGLIVIALFLLLAYGVYKIFLCNPTHGHQGFQGGDGSTGAWSGDYSNTGPPPPGFKPDYTA 236
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Sea Lamprey
Match: saraf (store-operated calcium entry-associated regulatory factor [Source:NCBI gene;Acc:767649])

HSP 1 Score: 161.77 bits (408), Expect = 1.163e-47
Identity = 97/268 (36.19%), Postives = 135/268 (50.37%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXXYTATE----KSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNF---------------------GFADETP---------PPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQ 785
            +++ L D+  LTL  G +T GRRS  VPQL CVGG +     + P  VQC N G DG  VQW+C+AD+G+  +FGS+ V+CEGYDYP+D YV +GSCGL Y+L      + +    +       K + +G+  ++  I V   +Y   L+   TG Q+   P   +                       F ++ P         PPP +   + AP E     R +RP  +  A    GPGFW+G+GAGGLMGYL GSR +     HQ
Sbjct:    1 DRLLLRDVSALTLHTGKMTAGRRSAAVPQLRCVGGCS----SFTPDVVQCLNRGHDGVDVQWECKADMGSSYRFGSISVSCEGYDYPDDPYVLRGSCGLEYSLVGGGGGAGTGGWRSNNPLPDGKGNGSGVLIILVLIGVAFGVYKLFLSGGGTGGQDVPYPPADDVHQQHASGLGTGPAYPPPPPPYSFKNQNPSSGYPAGSSPPPSY--GWAAPGE----PRMRRPPGAEQA--ATGPGFWSGMGAGGLMGYLLGSRNSGPTYHHQ 256
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Nematostella
Match: EDO48173 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK00])

HSP 1 Score: 126.331 bits (316), Expect = 1.940e-36
Identity = 57/96 (59.38%), Postives = 74/96 (77.08%), Query Frame = 3 
Query:   84 EKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGS 371
            EKIKL D+QVLTLK   +T GRRS PVPQL+CVGGSA C   + P+ VQC N GSDG+ VQW+C+AD+ ++ +FG + V+CEGY +P+D Y+ KGS
Sbjct:    2 EKIKLKDVQVLTLKANQMTNGRRSAPVPQLKCVGGSAGCS-AFRPQVVQCYNRGSDGYDVQWECKADMESQYRFGEIAVSCEGYSHPDDPYILKGS 96
[Close]
BLAST of Store-operated calcium entry-associated regulatory factor vs. Planmine SMEST
Match: SMESG000037963.1 (SMESG000037963.1)

HSP 1 Score: 591.652 bits (1524), Expect = 0.000e+0
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 3 
Query:   27 MDAINYFIAFLFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDXXXXXXXXXXXYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADETPPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 914
            MDAINYFIAFLFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDSSSHSSKSHQHYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTS FGFADETPPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR
Sbjct:    1 MDAINYFIAFLFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLECVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNCEGYDYPNDKYVFKGSCGLRYTLDSSSHSSKSHQHYTATEKSSWNGLYFVVFCIVVCLMIYYFCLAPNTTGFQNASMPSTSEFGFADETPPPPGFRPDYTAPPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAAFHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR 296
The following BLAST results are available for this feature:
BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SARAF5.668e-4134.09store-operated calcium entry associated regulatory... [more]
SARAF3.370e-3734.96store-operated calcium entry associated regulatory... [more]
SARAF3.526e-3434.34store-operated calcium entry associated regulatory... [more]
SARAF5.854e-2953.68store-operated calcium entry associated regulatory... [more]
SARAF8.706e-1431.44store-operated calcium entry associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
T06D8.91.459e-3633.01pep chromosome:WBcel235:II:11239407:11240988:1 gen... [more]
T06D8.91.459e-3633.01pep chromosome:WBcel235:II:11239718:11240988:1 gen... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
saraf2.864e-3658.65store-operated calcium entry-associated regulatory... [more]
saraf3.082e-3658.65store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SARAF5.023e-4438.93store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Saraf3.939e-3635.83store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|F7C1E2|SARAF_XENTR1.699e-4539.33Store-operated calcium entry-associated regulatory... [more]
sp|Q0IHF9|SARAF_XENLA4.322e-4437.46Store-operated calcium entry-associated regulatory... [more]
sp|Q96BY9|SARAF_HUMAN2.722e-4034.09Store-operated calcium entry-associated regulatory... [more]
sp|Q5R491|SARAF_PONAB2.222e-3933.99Store-operated calcium entry-associated regulatory... [more]
sp|Q08E24|SARAF_BOVIN8.827e-3934.28Store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9KD542.429e-5638.74Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A3S1CCW51.296e-5437.69Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A1D2N1N03.223e-5242.32Store-operated calcium entry-associated regulatory... [more]
A0A0L8GAG06.560e-5240.14Uncharacterized protein OS=Octopus bimaculoides OX... [more]
A0A218UMI41.649e-5036.43Store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
saraf2.356e-3434.91store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
saraf1.163e-4736.19store-operated calcium entry-associated regulatory... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO481731.940e-3659.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Store-operated calcium entry-associated regulatory factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000037963.10.000e+099.66SMESG000037963.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018884 ID=SMED30018884|Name=Store-operated calcium entry-associated regulatory factor|organism=Schmidtea mediterranea sexual|type=transcript|length=1012bp
CATAATTTAATACTTGTCGATTATTTATGGATGCAATTAATTACTTTATT
GCTTTTTTATTTTTTTCATCAACATTGGCGCATGAAAAAATAAAGTTAAC
TGACATTCAAGTGTTAACTTTGAAACACGGTTATTTGACAACTGGAAGAA
GATCACAACCAGTACCACAGCTAGAATGTGTTGGTGGCTCTGCTCAGTGT
GAGGATAAATACTTACCTCGCACTGTTCAGTGTACAAATATGGGCTCTGA
TGGATTTAGTGTTCAATGGAAATGCCAAGCTGATTTAGGAAATAAGTTAA
AATTTGGTTCAGTGGATGTGAACTGCGAAGGATATGATTATCCAAATGAT
AAATATGTATTTAAAGGATCGTGTGGATTGCGATATACATTGGATAGTAG
TAGCCACAGTAGTAAATCTCATCAACATTATACCGCTACTGAAAAATCAT
CATGGAATGGGCTTTATTTTGTTGTTTTCTGCATAGTTGTTTGCTTAATG
ATCTATTATTTCTGTTTGGCACCTAACACAACTGGATTTCAAAATGCTTC
TATGCCGTCGACATCCAATTTTGGATTTGCTGATGAAACCCCCCCTCCAC
CGGGATTCAGGCCTGATTATACCGCACCCCCAGAATACGAATCAATATAT
AGAAATAAAAGGCCATTTTCTAGCACTCATGCCGGAAATGAAAACGGGCC
TGGATTTTGGACAGGTCTAGGTGCCGGGGGACTGATGGGGTATTTGTTTG
GATCAAGGACTAATCAACAGGCCGCGTTTCATCAACCCACAGGATATTCT
GCTCCTCATACAGCTTGGCGGGAGGATTACTCCGCTCAACCTCGACATTT
CTCTGATTCCGACGAAACGCAAGAATACAGAACTGCTTCTGGATACGGTG
GAACTTCCAGGCGTTAAATTCGAATATTTAAAGGAACTTGAGTAATTGGG
ATTTCCTATGAACTGTTACATTATTTTATTATAAATATATCATTTATTGT
CTTTAAAAAAAA
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protein sequence of SMED30018884-orf-1

>SMED30018884-orf-1 ID=SMED30018884-orf-1|Name=SMED30018884-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=297bp
MDAINYFIAFLFFSSTLAHEKIKLTDIQVLTLKHGYLTTGRRSQPVPQLE
CVGGSAQCEDKYLPRTVQCTNMGSDGFSVQWKCQADLGNKLKFGSVDVNC
EGYDYPNDKYVFKGSCGLRYTLDSSSHSSKSHQHYTATEKSSWNGLYFVV
FCIVVCLMIYYFCLAPNTTGFQNASMPSTSNFGFADETPPPPGFRPDYTA
PPEYESIYRNKRPFSSTHAGNENGPGFWTGLGAGGLMGYLFGSRTNQQAA
FHQPTGYSAPHTAWREDYSAQPRHFSDSDETQEYRTASGYGGTSRR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030176integral component of endoplasmic reticulum membrane
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0006816calcium ion transport
GO:2001256regulation of store-operated calcium entry
Vocabulary: INTERPRO
TermDefinition
IPR009567SARAF
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009567Store-operated calcium entry-associated regulatory factorPFAMPF06682SARAFcoord: 18..296
e-value: 3.5E-78
score: 263.6
IPR009567Store-operated calcium entry-associated regulatory factorPANTHERPTHR15929UNCHARACTERIZEDcoord: 14..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 282..296
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.515
NoneNo IPR availableTMHMMTMhelixcoord: 224..243
NoneNo IPR availableTMHMMTMhelixcoord: 143..165