G-protein coupled receptor GRL101
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30018824 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Human
Match: RXFP1 (relaxin family peptide receptor 1 [Source:HGNC Symbol;Acc:HGNC:19718]) HSP 1 Score: 135.191 bits (339), Expect = 2.278e-31 Identity = 152/632 (24.05%), Postives = 269/632 (42.56%), Query Frame = 1
Query: 3946 GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK---------------NDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYL---MESDQLTWLTAVLMPINSSINPYLYTFS 5724
G F C + C+ C+G DC G DE++C + WS +K + E C + P +C C G ++ C + + ++ +++ +L F + L L + +N I + F GL +L L LS N I L F+ L +L+ L I DN ++ I F+ L S+ + L + L + D L +++ N I ++ +F+ +NL+ LV+ NK ++ ++ G +PL +L+ ++Y ++ C +C +D S+ E+LLA+ +VFVW + GNI VI R + + K I++L +D MGIYL +I D +R +Y +++ W S C + G A +S + SV + +TL+++ICI+ FR R + ++ L+W F+ + + F+++Y ++G+C S E Q +S+ + L I L F+I+ Y +F+ ++ TA I N K L + I F+ +F + L + +W+ ++PINS++NP LYT +
Sbjct: 30 GYFPCGNITKCLPQLLHCNGVDDC--GNQADEDNCG------DNNGWSLQFDKYFASYYKMTSQYPFEAETPE----CLVGSVPVQCLCQGLELDCDETNLRAVPSVSSNVTAMSLQWNLIRKLPPDCFKNYHDLQKLYLQNNKITSISIYAFRGLNSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPPTFYGLNSLILLVLMNNVLTRLPDKPLCQHMPRLHWLDLEGNHIHNLRNLTFISCSNLTVLVMRKNKINHLNENTFAPLQKLDEFSLEGIEISNIQQRMFRPLMNLS--HIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAVTCFGNIFVICMRPYIRSENKLYAMS--IISLCCADCLMGIYLFVIGGFDLKFRGEYNKHAQLWMESTHCQLVGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCVRPGKCRTITVLILIWITGFIVAFIPLSNKEFFKNYY-------GTNGVCFPLHSEDTESIGAQIYSVAIFLGINLAAFIIIVFSYGSMFYSVHQSAITATEI--RNQVKKEMILAKRFFFIVFTDALCWIPIFVVKFLSLLQVEIPGTITSWVVIFILPINSALNPILYTLT 636
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Human
Match: RXFP2 (relaxin family peptide receptor 2 [Source:HGNC Symbol;Acc:HGNC:17318]) HSP 1 Score: 132.109 bits (331), Expect = 1.874e-30 Identity = 128/491 (26.07%), Postives = 239/491 (48.68%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTL------VINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIF-----RTYRQHASKIKYLVFLVWFVWVI----SIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILML-----NDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
L GIF L +L + DN I + LF GL +L +L + NY+ LP + QL +D+ N I + + F + S+T ++L S + E++ + F + + L +N+S+N + + ++ F + L +L + N+N +P+K+L+ ++Y ++ C C +D S+ EDLLAN +++FVW + GN+ VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ A +S + SV + +TL++F+ I+F R ++ S I +++ F+ + + F +FY +G+C + + E + +S+ + L + L FLI+ Y+ +F C ++TA + + + A + ++ +CWIP+ ++ LS F + ++ +W+ +P+NS++NP LYT +
Sbjct: 200 LRPGIFKDLHQLTWLILDDNPITRISQRLFTGLNSLFFLSMVNNYLEALPKQMCAQMPQLNWVDLEGNRIKYLTNSTFLSCDSLTVLDLSSNTITELSPHLFKDL-KLLQKLNLSSNPLMYLHKNQFESLKQLQSLDLERIEIPNINTRMF----QPMKNLS--HIYFKNFRYCSYAPHVRICMPLTDGISSFEDLLANNILRIFVWVIAFITCFGNLFVIGMRSFI--KAENTTHAMSIKILCCADCLMGVYLFFVGIFDIKYRGQYQKYALLWMESVQCRLMGFLAMLSTEVSVLLLTYLTLEKFLVIVFPFSNIRPGKRQTSVILICIWMAGFLIAVIPFWNKDYFGNFY-------GKNGVCFPLYYDQTEDIGSKGYSLGIFLGVNLLAFLIIVFSYITMF---CSIQKTALQTTEVRNCFGREVAVANRFFFIVFSDAICWIPVFVVKILSLFRVEIPDT-MTSWIVIFFLPVNSALNPILYTLT 670
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Human
Match: RXFP1 (relaxin family peptide receptor 1 [Source:HGNC Symbol;Acc:HGNC:19718]) HSP 1 Score: 125.561 bits (314), Expect = 1.009e-28 Identity = 130/487 (26.69%), Postives = 223/487 (45.79%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF-QFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSF---VGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYL---MESDQLTWLTAVLMPINSSINPYLYTFS 5724
L G+F L +L I DN + + F GL +L L+L N + LP Q + +L L + N I + F L + ++L S K+ + F + E L +N+S N I+ I + F V + +LS I ++ M +PL +L+ ++Y ++ C +C +D S+ E+LLA+ +VFVW + GNI VI R + + K I++L +D MGIYL +I D +R +Y +++ W S C + G A +S + SV + +TL+++ICI+ FR R + ++ L+W F+ + + F+++Y ++G+C S E Q +S+ + L I L F+I+ Y +F+ ++ TA I N K L + I F+ +F + L + +W+ ++PINS++NP LYT +
Sbjct: 57 LKPGVFEDLHRLEWLIIEDNHLSRISPPTFYGLNSLILLVLMNNVLTRLPDKPLCQHMPRLHWLVMRKNKINHLNENTFAPLQKLDELDLGSNKIENLPPLIFKDLKE-LSQLNLSYNPIQKIQANQFDYLVKLKSLSLEGIEISNIQQRMF-RPLMNLS--HIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAVTCFGNIFVICMRPYIRSENKLYAMS--IISLCCADCLMGIYLFVIGGFDLKFRGEYNKHAQLWMESTHCQLVGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCVRPGKCRTITVLILIWITGFIVAFIPLSNKEFFKNYY-------GTNGVCFPLHSEDTESIGAQIYSVAIFLGINLAAFIIIVFSYGSMFYSVHQSAITATEI--RNQVKKEMILAKRFFFIVFTDALCWIPIFVVKFLSLLQVEIPGTITSWVVIFILPINSALNPILYTLT 528
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Human
Match: RXFP2 (relaxin family peptide receptor 2 [Source:HGNC Symbol;Acc:HGNC:17318]) HSP 1 Score: 126.716 bits (317), Expect = 1.273e-28 Identity = 131/514 (25.49%), Postives = 241/514 (46.89%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTF--------LGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTL------VINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIF-----RTYRQHASKIKYLVFLVWFVWVI----SIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILML-----NDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
L GIF L +L + DN I + LF GL +L +L + NY+ LP + QL +D+ N I + + F + S+T + LP ++ + + TF L +S N I+ +N+S+N + + ++ F + L +L + N+N +P+K+L+ ++Y ++ C C +D S+ EDLLAN +++FVW + GN+ VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ A +S + SV + +TL++F+ I+F R ++ S I +++ F+ + + F +FY +G+C + + E + +S+ + L + L FLI+ Y+ +F C ++TA + + + A + ++ +CWIP+ ++ LS F + ++ +W+ +P+NS++NP LYT +
Sbjct: 200 LRPGIFKDLHQLTWLILDDNPITRISQRLFTGLNSLFFLSMVNNYLEALPKQMCAQMPQLNWVDLEGNRIKYLTNSTFLSCDSLTVLFLPRNQIGFVPEKTFSSLKNLGELDLSSNTITELSPHLFKDLKLLQKLNLSSNPLMYLHKNQFESLKQLQSLDLERIEIPNINTRMF----QPMKNLS--HIYFKNFRYCSYAPHVRICMPLTDGISSFEDLLANNILRIFVWVIAFITCFGNLFVIGMRSFI--KAENTTHAMSIKILCCADCLMGVYLFFVGIFDIKYRGQYQKYALLWMESVQCRLMGFLAMLSTEVSVLLLTYLTLEKFLVIVFPFSNIRPGKRQTSVILICIWMAGFLIAVIPFWNKDYFGNFY-------GKNGVCFPLYYDQTEDIGSKGYSLGIFLGVNLLAFLIIVFSYITMF---CSIQKTALQTTEVRNCFGREVAVANRFFFIVFSDAICWIPVFVVKILSLFRVEIPDT-MTSWIVIFFLPVNSALNPILYTLT 694
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Human
Match: RXFP1 (relaxin family peptide receptor 1 [Source:HGNC Symbol;Acc:HGNC:19718]) HSP 1 Score: 125.561 bits (314), Expect = 2.940e-28 Identity = 127/510 (24.90%), Postives = 229/510 (44.90%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF-QFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK---------------NDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYL---MESDQLTWLTAVLMPINSSINPYLYTFS 5724
L G+F L +L I DN + + F GL +L L+L N + LP Q + +L LD+ N I + + F + ++T + + K+ +N+ TF + + L +++ +N I ++ F + LS L ++ N +++ G +PL +L+ ++Y ++ C +C +D S+ E+LLA+ +VFVW + GNI VI R + + K I++L +D MGIYL +I D +R +Y +++ W S C + G A +S + SV + +TL+++ICI+ FR R + ++ L+W F+ + + F+++Y ++G+C S E Q +S+ + L I L F+I+ Y +F+ ++ TA I N K L + I F+ +F + L + +W+ ++PINS++NP LYT +
Sbjct: 216 LKPGVFEDLHRLEWLIIEDNHLSRISPPTFYGLNSLILLVLMNNVLTRLPDKPLCQHMPRLHWLDLEGNHIHNLRNLTFISCSNLTVLVMRKNKINHLNENTFAPL-QKLDELDLGSNKIENLPPLIFKDLKELSQLNLSYNPIQKIQANQFDYLVKLKSLSLEGIEISNIQQRMFRPLMNLS--HIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAVTCFGNIFVICMRPYIRSENKLYAMS--IISLCCADCLMGIYLFVIGGFDLKFRGEYNKHAQLWMESTHCQLVGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCVRPGKCRTITVLILIWITGFIVAFIPLSNKEFFKNYY-------GTNGVCFPLHSEDTESIGAQIYSVAIFLGINLAAFIIIVFSYGSMFYSVHQSAITATEI--RNQVKKEMILAKRFFFIVFTDALCWIPIFVVKFLSLLQVEIPGTITSWVVIFILPINSALNPILYTLT 711
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Celegans
Match: lrp-1 (Low-density lipoprotein receptor-related protein [Source:UniProtKB/Swiss-Prot;Acc:Q04833]) HSP 1 Score: 79.337 bits (194), Expect = 3.176e-14 Identity = 73/233 (31.33%), Postives = 113/233 (48.50%), Query Frame = 1
Query: 3532 EKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKD--CDIC--------NGFKCLSSL-CIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACP--LLEHLQNCISFQCPG-LFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDI 4185
E+LCS + T++QC+N +CI +C+ +DC+D SDE D D C N KC ++ CI + + D DC S ED K+ K +C++ +C N+ C+ S + C E L+ C + C F C+ + CI ++++CDG DC DG DED+E C ++C FRC + C+ ++ C+G SDC +G DE C +
Sbjct: 1051 EQLCS----SNSTQFQCKNGRCIPKEWKCDGENDCLDESDEIDEKGDKCFHETECAENTIKCRNTKKCIPAQYGCDGDNDCGDYSDEDVKYCKDG-------QKPVCAAKKFQC--DNHRCIPEQWKCDSDNDCGDGSDEKLEMCGNATCAANQFSCANGR-CIPIYWLCDGDNDCYDGTDEDKERCPPVQCSALQFRCANGRQ----CVPLRNHCDGQSDCEDGSDEDSCAV 1265 HSP 2 Score: 79.337 bits (194), Expect = 3.454e-14 Identity = 78/289 (26.99%), Postives = 118/289 (40.83%), Query Frame = 1
Query: 3463 NDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQC-----QNKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTC--LYHSNNLL-SRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDE-----------DEESCKTLKC-PYNFRCWSFNH-----------------------SFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPT 4200
ND + I CD DC +G DEK C + C + +WQC + CI +RC+ ++DC D SDEKD CP S+++ S ++ + +C+ +CF + C Y +L +D+C + C +Q F+C+ CI+ +VCDG+KDC DG DE +E CK +C P FRC ++ C++ LC+G DC++G DEQ C CP+
Sbjct: 61 NDGKCIRTEWKCDGSGDCSDGEDEKDCPHPGC-KSDQWQCDTYTWHSVSCIAEYQRCDNITDCADGSDEKD--------------------------CPASTVD---CSSQNVF--------MCAD-GRQCFDVSKKCDGKYDCRDLSDEKDSCS--RNHTACFQYQ----FRCADKTQCIQKSWVCDGSKDCADGSDEPDTCEFKKCTANEFQCKNKRCQPRKFRCDYYDDCGDNSDEDECGEYRCPPGKWNCPGTGHCIDQLKLCDGSKDCADGADEQQCSQNLCPS 304 HSP 3 Score: 78.9518 bits (193), Expect = 4.120e-14 Identity = 107/372 (28.76%), Postives = 153/372 (41.13%), Query Frame = 1
Query: 3181 KNSSFYFLQF*RRT*ERVCICERSSQTIKSLINASKEQINGFWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNR---NDNQFISWNRYCDFIEDCKNGTDEKLCSYSR--CAELTEWQCQN-KQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLS--------SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEES---CKTLKC-------PYNFRCWSFNHSFLLCLNHKDLCNGISDCSE-GEDEQYCDIFQCPTKCKCFG 4218
+ S+F+ +Q R I S T S+ N N F C D I + CD DC E DEKDC P + Q+ C+ + I+ + CD I DC +G+DEK C S C+ + C + +QC DVSK+C+ DC D SDEKD N C + CI + D DC S E + + C N + K+ +C C Y+ D C C ++CP G + C +CI+ +CDG+KDC DG DE + S C +L C P+ C + + L C+ I++C+E G +Q C + C C G
Sbjct: 22 QQSTFHSIQVDSPPSVRSRIISASVNTASSVCNE-----NDFRCN--DGKCIRTEWKCDGSGDCSDGE-DEKDC-----PHPGCKSDQWQ-CDTYTWHSVSCIAEYQRCDNITDCADGSDEKDCPASTVDCSSQNVFMCADGRQCFDVSKKCDGKYDCRDLSDEKDSCSRNHTACFQYQFRCADKTQCIQKSWVCDGSKDCADGSDEPDTCEFKKCTANEFQCKN------KRCQPRKFRCDYY-------DDCGDNSDEDECGEYRCPPGKWNCPGTGHCIDQLKLCDGSKDCADGADEQQCSQNLCPSLGCQAGCHPSPHGGEC-TCPSGYKLDDRFHRTCSDINECAEFGYCDQLCANHRPGFTCSCLG 365 HSP 4 Score: 74.7146 bits (182), Expect = 8.600e-13 Identity = 70/228 (30.70%), Postives = 101/228 (44.30%), Query Frame = 1
Query: 3493 YCDFIEDCKNGTDEKLCSYSRCAELTEWQCQ-NKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLE---HLQNCISFQC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC--PYNFRCWSFNHSFLLCLNHKDLCNGISDCS 4155
+C+ +DC++ +DE C ++ E++C + +CI C+ +DC DNSDE D C G + C S F +D PGS + D I D C +C + TC+ + CP H ++ C P F+C+ CI ++CDG DC D DE C++ +C P FRC HS LCLN LCNG +DC
Sbjct: 3812 FCNGQDDCRDNSDEDKQRCPTCDDVGEFRCATSGKCIPRRWMCDTENDCGDNSDELDAS-CGG---TTRPCSESEFRCNDGKCIPGSKVCDGTIQCSDGLDESQCTLRRCLPGHRQC--DDGTCIAEHKWCDRKKDCPNAADELHCEDVSRRTCSPFEFECAN-SVCIPRKFMCDGDNDCGDNSDETSSECRSAQCDPPLRFRC---AHS-RLCLNILQLCNGFNDCG 4028 HSP 5 Score: 74.3294 bits (181), Expect = 1.234e-12 Identity = 75/253 (29.64%), Postives = 104/253 (41.11%), Query Frame = 1
Query: 3463 NDNQFISWNRYCDFIEDCKNGTDEK--LCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDIC-----NGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAE---IKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESC------KTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQ 4173
D I CD DC + +DE C+ C E C N++CID +K+CN + DC D SDE DC F C + CI D DC + DE ++ C P + +K C +T N C Q + C + + D L C++ QC + CI + CDG DC DG DE +C K Y F+C L C++ +C+G DC +G DE
Sbjct: 2801 GDGSCILLGATCDSKPDCADASDENPNYCNTRSCPEDYNL-CTNRRCIDSAKKCNHIDDCGDGSDELDCPSAVACAEGTFPCSNGHCINQTKVCDGHNDCHDEQVSDESLAT--CPGLPIDCRGVKVRCPNT-NICIQPADLCDGYDDCGDKADENQLF-----CMNQQCAQHYVRCPSGRCIPETWQCDGDNDCSDGWDETHTNCTDTAGKKICVGDYLFQC-----DNLKCISRAFICDGEDDCGDGSDEH 3039 HSP 6 Score: 69.707 bits (169), Expect = 3.203e-11 Identity = 86/292 (29.45%), Postives = 127/292 (43.49%), Query Frame = 1
Query: 3352 CDFHIDCLISENDEKDCIVKVKSKPMNE---FQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDE--KLCSYSR---CAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDIC-------NGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSN--NLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYN-FRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE 4170
CD DCL DE D I + K +E + + C RN + I CD DC + +DE K C + CA ++QC N +CI +C+ +DC D SDEK ++C N F C + CI + D DC + ED KE C + +C N C S+ + D+C + ++C P F+C CI + CDG +DC DG DE + C + + C + F+C + C+ + LC+G +DC +G DE
Sbjct: 1075 CDGENDCL----DESDEIDEKGDKCFHETECAENTIKC-RNTKKCIPAQYGCDGDNDCGDYSDEDVKYCKDGQKPVCA-AKKFQCDNHRCIPEQWKCDSDNDCGDGSDEK-LEMCGNATCAANQFSCANGRCIPIYWLCDGDNDCYDGTDED----KERCPPVQCSALQFRCANGRQCVPLRNHCDGQSDCEDGSDEDSCAVT--AESCT----PDQFKCVSSGLCIPASWKCDGQQDCDDGSDEPKFGCTSGRQCSSDQFKCGNGR-----CILNNWLCDGENDCGDGSDE 1344 HSP 7 Score: 67.3958 bits (163), Expect = 1.551e-10 Identity = 73/258 (28.29%), Postives = 110/258 (42.64%), Query Frame = 1
Query: 3439 QQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEK---LCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD-ICNG--FKCLSS-LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQ-----SNNTCLYHS-NNLLSRDACPLLEHLQNCISFQCP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE 4170
+QF C +D + I YCD DC++G+DE +C C + E+QC N C + C+ DC D+SDE++CD C+ FKC ++ CI F +D+ + D E +LC S C +NN C+ + D + C +C G +CS CI C+G DC D DED++ C T FRC + C+ + +C+ +DC + DE
Sbjct: 3630 RQFA-CGGDDAKCIPKLWYCDGEPDCRDGSDEPGESICGQRICP-VGEFQCTNHNCTRPFQICDGNDDCGDSSDEQNCDKACDPWMFKCAATGRCIPRRFTC---------DGDDDCGDRSD------EADTLCMSAERNCTAEEFRCNNNKCIAKAWRCDNDDDCGDGSDETPECAQIECKKGWTRCSSSYRCIPNWAFCNGQDDCRDNSDEDKQRCPTCDDVGEFRCATSGK----CIPRRWMCDTENDCGDNSDE 3866 HSP 8 Score: 59.3066 bits (142), Expect = 4.551e-8 Identity = 61/201 (30.35%), Postives = 91/201 (45.27%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDE--KLCSYSRCAELTEWQCQN-KQCIDVSKRCNKVSDCIDNSDEKDCDIC------NGFKCLSS-LCIASMFEMDDIVDCPGSSMEDEI--ISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQC-SKMKYCIELHYVCDGTKDCPDGEDEDEESCKTL 4059
CD DC +GTDE + C +C+ L +++C N +QC+ + C+ SDC D SDE C + + FKC+SS LCI + ++ D DC S E + S C + + + +N N C S+ R C + + C F+ C + + CI LH +CDG CP G DE + L
Sbjct: 1206 CDGDNDCYDGTDEDKERCPPVQCSAL-QFRCANGRQCVPLRNHCDGQSDCEDGSDEDSCAVTAESCTPDQFKCVSSGLCIPASWKCDGQQDCDDGSDEPKFGCTSGRQCSSDQFKCGNGRCILNNWLCDGENDCGDGSDESSER-GCKTSMNARKC-PFE---HVACENDQETCIPLHQLCDGKTHCPGGTDEGGRCARDL 1400 HSP 9 Score: 57.7658 bits (138), Expect = 1.062e-7 Identity = 75/301 (24.92%), Postives = 110/301 (36.54%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD-----ICNGFK--CLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQC--PGLFQCSKMKYCIELHYVCDGTKDCP--DGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFG 4218
+CD DC +DE D ++P +E + CN D + I ++ CD C +G DE C+ RC QC + CI K C++ DC + +DE C+ C+ F+ C +S+CI F D DC N E S C S QC P F+C+ + C+ + +C+G DC D DE C + + F + C+N C+ DC + DE C T C+ FG
Sbjct: 3853 MCDTENDCG-DNSDELDASCGGTTRPCSESE--FRCN--DGKCIPGSKVCDGTIQCSDGLDESQCTLRRCLP-GHRQCDDGTCIAEHKWCDRKKDCPNAADELHCEDVSRRTCSPFEFECANSVCIPRKFMCD---------------GDNDCGDNSDETSSECRSA------------------------------------QCDPPLRFRCAHSRLCLNILQLCNGFNDCGPNDFSDEHLSMCSSFSEYGDCSSDQFKCANGKCVNGTVACDRKDDCGDASDEIGCSKHGGKTSCEAFG 4096 HSP 10 Score: 56.9954 bits (136), Expect = 1.802e-7 Identity = 84/301 (27.91%), Postives = 121/301 (40.20%), Query Frame = 1
Query: 3346 KVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEK--LCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD--------ICNG---FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLE----HLQNCISFQC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDL-----CNGISDCSEGEDEQYC 4179
KVCD H DC E + + P++ + C N N I CD +DC + DE C +CA+ +C + +CI + +C+ +DC D DE + IC G F+C + CI+ F D DC S E S+ C + +++ K Q C+ + C E L +C G F+CS ++CI + CDG DC DG DE C Y+ S F C NHK + C+G DC +G DE+ C
Sbjct: 2891 KVCDGHNDCH-DEQVSDESLATCPGLPIDCRGVKVRCP-NTNICIQPADLCDGYDDCGDKADENQLFCMNQQCAQHYV-RCPSGRCIPETWQCDGDNDCSDGWDETHTNCTDTAGKKICVGDYLFQCDNLKCISRAFICDGEDDCGDGSDEH---SRHGCGNRTCTDQEFHCTSNAKLAQPKYECIPRAWLCDGDVTCAGGEDESTELCKTEKKECNKGEFRCSN-QHCIHSTWECDGDNDCLDGSDE------HANCTYS----SCQPDFFQCANHKCVPNSWKCDGNDDCEDGSDEKDC 3174
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Celegans
Match: fshr-1 (FSHR (Mammalian follicle stimulating hormone receptor) homolog [Source:UniProtKB/TrEMBL;Acc:L8EC40]) HSP 1 Score: 75.0998 bits (183), Expect = 4.967e-13 Identity = 45/187 (24.06%), Postives = 98/187 (52.41%), Query Frame = 1
Query: 4840 TCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRI-SVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTY--------RQHASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGLCLS 5373
TC + D + CE+++ +++ VW + + +VGNI I+W + + +K+ + ++N++++DM GIYL ++A AD +Y ++++W++ C AG+ A +++ +G+I++ F+ +Y R+ + K+ L+ + +++ I +A+ F S E S +CL
Sbjct: 412 TCYPQPDALNPCENIVGYPFLRIAVWVVCLAAIVGNI--IVWALLGIVYEKRMRMHYLYMINMSVADMVTGIYLAVLAIADAKMSDEYYRHAVWWQTGWGCRAAGFLAVFASE-----LGIISM--FLIAFEMSYNTRQSFRGRRLSPKVGVLLMIGGWLFAIIMAILPWFDVSSYSE---SSVCLP 586
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Celegans
Match: fshr-1 (FSHR (Mammalian follicle stimulating hormone receptor) homolog [Source:UniProtKB/TrEMBL;Acc:L8EC40]) HSP 1 Score: 74.7146 bits (182), Expect = 5.462e-13 Identity = 45/187 (24.06%), Postives = 98/187 (52.41%), Query Frame = 1
Query: 4840 TCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRI-SVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTY--------RQHASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGLCLS 5373
TC + D + CE+++ +++ VW + + +VGNI I+W + + +K+ + ++N++++DM GIYL ++A AD +Y ++++W++ C AG+ A +++ +G+I++ F+ +Y R+ + K+ L+ + +++ I +A+ F S E S +CL
Sbjct: 412 TCYPQPDALNPCENIVGYPFLRIAVWVVCLAAIVGNI--IVWALLGIVYEKRMRMHYLYMINMSVADMVTGIYLAVLAIADAKMSDEYYRHAVWWQTGWGCRAAGFLAVFASE-----LGIISM--FLIAFEMSYNTRQSFRGRRLSPKVGVLLMIGGWLFAIIMAILPWFDVSSYSE---SSVCLP 586
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Celegans
Match: T13C2.6 (pep chromosome:WBcel235:II:6783427:6788255:1 gene:WBGene00020481.1 transcript:T13C2.6b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T13C2.6) HSP 1 Score: 74.3294 bits (181), Expect = 8.677e-13 Identity = 73/259 (28.19%), Postives = 99/259 (38.22%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCS--YSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDE------------KDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDE-----ESCKTLK--CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCK 4209
CD DC NG DE+ C+ S+C + +QC N CI + C+ DC D SDE C +KC S CI S + D VDC S E N T + H+ C L E F C CI +VCDG DC DG DED+ CK+ + CP ++ + ++C+ CNG DC +G DE+ C++ P C+
Sbjct: 146 CDGQRDCTNGKDEQNCTSKTSKCPD-NNFQCSNGNCIFKNWVCDGEEDCSDGSDELLTAPSNCNRTVNQCPPGEMWKCGSGECIPSRWRCDAEVDCKDHSDE-----------------------------KNCTAIQHT--------CKLAEE------------FACKASHNCINKAFVCDGELDCSDGSDEDDCADVRTECKSGERTCPASYGAYGAESGHVVCIPASSWCNGEEDCPDGGDEKECNM-TAPVTCQ 353 HSP 2 Score: 58.9214 bits (141), Expect = 3.594e-8 Identity = 65/230 (28.26%), Postives = 94/230 (40.87%), Query Frame = 1
Query: 3553 RCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSN-KCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGL------FQCSKM----KYCIELHYVCDGTKDCPDGEDEDEESCKTLKCP-YNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKC 4206
+C +QCQ+ +CI +S RC+ DC + DEK+C I S +C A + E+KS SS KC + C + D FQC + FQC ++ CI +VCDG +DC +G+DE + KT KCP NF+C + N C+ +C+G DCS+G DE + P+ C
Sbjct: 31 KCDATNSFQCQDGRCIPMSWRCDGDIDCQNEEDEKNCPI-------SEVCGAEEHKC-------------------------GEVKSARSSLERFKCIPNKWVCDGEFDCEDKSD------------EFQCKNVSCQEKQFQCEELSGDYSLCIPETWVCDGQRDCTNGKDEQNCTSKTSKCPDNNFQCSNGN-----CIFKNWVCDGEEDCSDGSDE----LLTAPSNC 207
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Celegans
Match: T13C2.6 (pep chromosome:WBcel235:II:6783427:6788255:1 gene:WBGene00020481.1 transcript:T13C2.6a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T13C2.6) HSP 1 Score: 73.9442 bits (180), Expect = 1.010e-12 Identity = 73/259 (28.19%), Postives = 99/259 (38.22%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCS--YSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDE------------KDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDE-----ESCKTLK--CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCK 4209
CD DC NG DE+ C+ S+C + +QC N CI + C+ DC D SDE C +KC S CI S + D VDC S E N T + H+ C L E F C CI +VCDG DC DG DED+ CK+ + CP ++ + ++C+ CNG DC +G DE+ C++ P C+
Sbjct: 144 CDGQRDCTNGKDEQNCTSKTSKCPD-NNFQCSNGNCIFKNWVCDGEEDCSDGSDELLTAPSNCNRTVNQCPPGEMWKCGSGECIPSRWRCDAEVDCKDHSDE-----------------------------KNCTAIQHT--------CKLAEE------------FACKASHNCINKAFVCDGELDCSDGSDEDDCADVRTECKSGERTCPASYGAYGAESGHVVCIPASSWCNGEEDCPDGGDEKECNM-TAPVTCQ 351 HSP 2 Score: 57.7658 bits (138), Expect = 9.670e-8 Identity = 58/198 (29.29%), Postives = 90/198 (45.45%), Query Frame = 1
Query: 3655 KDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKC--FQSNNTCLYHSNNLLSRDAC-PLLEHLQNCISFQCPGL------FQCSKMK----YCIELHYVCDGTKDCPDGEDEDEESCKTLKCP-YNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKC 4206
+ CD N F+C CI + D +DC + EDE ++C K +C + +KC +S + L + ++ C + FQC + FQC ++ CI +VCDG +DC +G+DE + KT KCP NF+C + N C+ +C+G DCS+G DE + P+ C
Sbjct: 30 RKCDATNSFQCQDGRCIPMSWRCDGDIDC--QNEEDE----KNCPK-------VCGAEEHKCGEVKSARSSLERFKCIPNKWVCDGEFDCEDKSDEFQCKNVSCQEKQFQCEELSGDYSLCIPETWVCDGQRDCTNGKDEQNCTSKTSKCPDNNFQCSNGN-----CIFKNWVCDGEEDCSDGSDE----LLTAPSNC 205
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Fly
Match: Lgr3 (gene:FBgn0039354 transcript:FBtr0084899) HSP 1 Score: 126.331 bits (316), Expect = 8.742e-29 Identity = 128/543 (23.57%), Postives = 226/543 (41.62%), Query Frame = 1
Query: 3940 CPGL-FQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGED-------------EQYCDIFQCPTKCKCF-GYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDN-----PITTIESFAF-------HNLISITHIELPSMK-LYE----------INDYTF--------LGMSENLIS---------------INMSNNDIRSISQDSFVGVNNL-------------STLVINVNKNDIAMSGKPLKHL------TVKNVYTNVYK---ICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVWFVWVISIAL 5313
CP F C C+ +CD DC D DE+ C L ++ + + L + +S+ S + CDI P C+C G + C + T I+ ++L L IF+ F L L + N+I + G F GLF+L L LS N + LP F L LQ L + +N P+ + F N +++ +++LP + LYE I + TF L + NLI+ I + N I+ +S ++F+ L S+L+ +N + + ++G H+ +++N+ +Y C C +D S+ +DLL+ ++ W M + GN++V+ W + R + + ++I NLA++DM MG YL+ I D YR++Y + + W +S C++ G A S++ S+ + ++L+RF+ I FR +R +++ +L + +W+ + L
Sbjct: 32 CPAASFACDNGTLCVPRRQMCDSRNDCADSSDENPVECGLLYGSKEIADKIVRNA--IEKKQQRLISAVSNASGADSTTSMVPRNQSLTLNMTCDIVTYPKACQCGQGTILYCGRYAKLRRFPRLSSEVTNLIIIRNNLTLRDNIFANFTRLQKLTLKYNNISRVPLGSFSGLFHLERLELSHNNVSHLPHGVFLGLHSLQWLFLVNNHLHHLPVEQLRFFRRLEWLVLSRNRLTLRNVQLPKIPTLYEVYLDFNRIEYIGEETFSQLDNLHLLDLQHNLITHIHGRAFANLTNMRDIRLVGNPIKELSGETFLHNTRLEALSLALMPIHISSSLMEPLNISFLNLTGIRYDHIDFEAINSMRNLTYIIYDRFFYCSMTPRVRMCKPSTDGVSSFQDLLSKPVLRYSAWVMATLTIAGNVLVL-WGRFIYRDENVAVT-MVIRNLALADMLMGFYLVTIGVQDYRYRNEYYKVVLDWITSWQCTLIGTLAVSSSEVSMLILAFMSLERFLLIADPFRGHRSIGNRVMWLALIC--IWITGVGL 568
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Fly
Match: Lgr4 (gene:FBgn0085440 transcript:FBtr0300364) HSP 1 Score: 125.946 bits (315), Expect = 1.010e-28 Identity = 104/376 (27.66%), Postives = 178/376 (47.34%), Query Frame = 1
Query: 4285 LTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYI-RILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLT-VKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIALF-----------QHFYHSKMDECESSGLCLS 5373
L + + H L F L L++ N I +F L NL L L++N++ R+ P + L L L + N I I + F + ++ L +L I D TF +S NL ++++ N I ++F + NLS+L++ N+ + + LK+LT + +Y + + +C + + C D S+ LL N+ ++ VW M +VGN++V++ R + + + + +LA SD MGIYL +IA AD +R +YI+ W+ S C+ AG+ +T S QSS + L+T DR + + + K++ +V + +W IS L HFY ++G+CLS
Sbjct: 262 LILARNHLHHLKRSDFLNLQKLQELDLRGNRIGNFEAEVFARLPNLEVLYLNENHLKRLDPDRFPRTLLNLHTLSLAYNQIEDIAANTF-PFPRLRYLFLAGNRLSHIRDETFCNLS-NLQGLHLNENRIEGFDLEAFACLKNLSSLLLTGNRFQ-TLDSRVLKNLTSLDYIYFSWFHLCSAAMNVRVCDPHGDGISSKLHLLDNQILRGSVWVMASIAVVGNLLVLLGRY-FYKSRSNVEHSLYLRHLAASDFLMGIYLTLIACADISFRGEYIKYEETWRHSGVCAFAGFLSTFSCQSSTLLLTLVTWDRLMSVTRPLKPRDTEKVR-IVLRLLLLWGISFGLAAAPLLPNPYFGSHFY-------GNNGVCLS 625
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Fly
Match: Lgr4 (gene:FBgn0085440 transcript:FBtr0340300) HSP 1 Score: 125.946 bits (315), Expect = 1.160e-28 Identity = 104/376 (27.66%), Postives = 178/376 (47.34%), Query Frame = 1
Query: 4285 LTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYI-RILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLT-VKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIALF-----------QHFYHSKMDECESSGLCLS 5373
L + + H L F L L++ N I +F L NL L L++N++ R+ P + L L L + N I I + F + ++ L +L I D TF +S NL ++++ N I ++F + NLS+L++ N+ + + LK+LT + +Y + + +C + + C D S+ LL N+ ++ VW M +VGN++V++ R + + + + +LA SD MGIYL +IA AD +R +YI+ W+ S C+ AG+ +T S QSS + L+T DR + + + K++ +V + +W IS L HFY ++G+CLS
Sbjct: 265 LILARNHLHHLKRSDFLNLQKLQELDLRGNRIGNFEAEVFARLPNLEVLYLNENHLKRLDPDRFPRTLLNLHTLSLAYNQIEDIAANTF-PFPRLRYLFLAGNRLSHIRDETFCNLS-NLQGLHLNENRIEGFDLEAFACLKNLSSLLLTGNRFQ-TLDSRVLKNLTSLDYIYFSWFHLCSAAMNVRVCDPHGDGISSKLHLLDNQILRGSVWVMASIAVVGNLLVLLGRY-FYKSRSNVEHSLYLRHLAASDFLMGIYLTLIACADISFRGEYIKYEETWRHSGVCAFAGFLSTFSCQSSTLLLTLVTWDRLMSVTRPLKPRDTEKVR-IVLRLLLLWGISFGLAAAPLLPNPYFGSHFY-------GNNGVCLS 628
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Fly
Match: LpR2 (gene:FBgn0051092 transcript:FBtr0336451) HSP 1 Score: 89.7373 bits (221), Expect = 2.112e-17 Identity = 85/251 (33.86%), Postives = 115/251 (45.82%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELT----EWQCQNKQ--CIDVSKRCNKVSDCIDNSDEKDCD-ICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNC----ISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKC 4206
CD DC +G+DE S S+C T E+ C++ + CI +S C++ DC D SDE C+ C F C + CI + F+ DD DC S E K C N + S C + C S+ D +QNC ++F G FQCS C+ +VCDG DCPDGEDE + +C + C P F+C + C+ CNG DC++G DE CDI P C
Sbjct: 53 CDRENDCADGSDE---STSQCRSHTCSPEEFACKSGEGECIPLSWMCDQNKDCRDGSDEAQCNRTCRSDEFTCGNGRCIQNRFKCDDDDDCGDGSDEKNCGEKAKCGSN------FFACKSGPCIPNQWVCDGDSDCRNGED------EMQNCTVSLLNFCQAGEFQCSDRITCLHKSWVCDGEADCPDGEDESQSNCLKVSCRPDQFQC-----NDQSCIAGHLTCNGKRDCADGSDEIMCDISATPRTC 283
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Fly
Match: LpR2 (gene:FBgn0051092 transcript:FBtr0336452) HSP 1 Score: 89.3521 bits (220), Expect = 2.520e-17 Identity = 85/251 (33.86%), Postives = 115/251 (45.82%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELT----EWQCQNKQ--CIDVSKRCNKVSDCIDNSDEKDCD-ICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNC----ISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKC 4206
CD DC +G+DE S S+C T E+ C++ + CI +S C++ DC D SDE C+ C F C + CI + F+ DD DC S E K C N + S C + C S+ D +QNC ++F G FQCS C+ +VCDG DCPDGEDE + +C + C P F+C + C+ CNG DC++G DE CDI P C
Sbjct: 53 CDRENDCADGSDE---STSQCRSHTCSPEEFACKSGEGECIPLSWMCDQNKDCRDGSDEAQCNRTCRSDEFTCGNGRCIQNRFKCDDDDDCGDGSDEKNCGEKAKCGSN------FFACKSGPCIPNQWVCDGDSDCRNGED------EMQNCTVSLLNFCQAGEFQCSDRITCLHKSWVCDGEADCPDGEDESQSNCLKVSCRPDQFQC-----NDQSCIAGHLTCNGKRDCADGSDEIMCDISATPRTC 283
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Zebrafish
Match: rxfp2b (relaxin family peptide receptor 2b [Source:ZFIN;Acc:ZDB-GENE-110408-27]) HSP 1 Score: 147.902 bits (372), Expect = 1.408e-35 Identity = 183/686 (26.68%), Postives = 302/686 (44.02%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWS--FNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISI-------THIE-LPSMKL-----------YEINDYTFLGMS-----ENLISINMSNNDIRSISQDSFVGV----------NNLSTLVINVNKN-----DIAMSGKPLKHL-------------------TVKNVYTNVYKIC-------------CSLASY-GTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIAL----------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXX-XXXXXXXXXXXVTGYVLIFFH----SCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSN 5727
CP G F C + C+ C+ KDCP+G DED C N W+ F+ +F L +DC + EQY D+ C+C +I C + +I ++ +L++ L F ++I L L + DN I + F GL+ L+ L LS+N I +L F+ L++L+ L + DNPITTI + F L S+ T +E LPS +L +E N T LG+S E+L +++ N I+S+ +++F + N++ L + + K+ + +S PL H+ + N+ T++++ CS A + C SD S+ EDLLAN ++V VW M GN+ VI R +V R + + I L +D MG+YL + D +R +Y +N+ W S+ C G+ A +S++ SV + +TL++F+ I+F SK + LV +W++ I++ F ++Y +G+C S + E + +S + L LL +IV Y +F+ R GR + D A + ++ LCWIPI ++ LS + +W+ ++PINS++NP LYT +
Sbjct: 39 CPLGHFPCGNVSICLPQVLHCNNQKDCPNGADED-------NCGDN-SGWADLFDRTF----KRGYLQELATDC---QVEQYPDV------CRCINTEIHCVHASLRSIPQVSANVTSLSLKSNKIRTLPDEAFIKYIKLQRLFLQDNCINTVSIQAFSGLYKLQKLSLSQNSISLLSPGVFRDLRKLKWLILDDNPITTIAANTFAGLSSLFFLSMVNTSLEALPSARLCAHMPFLSWLDFEGNSITTLGLSTLLDCEHLTVLSLRANLIKSLPENTFQSLRMMGDLDLSNNSIKELPVGIFKDLRSLQSLNLSQNPLDHIHPGQFNHLIQLQSLGLEGVEIPNIQTSMFRPMDNLSYIYFKKFQYCSYAPHVRKCKPNSDGLSSFEDLLANVVLRVSVWVMAFITCFGNLFVIGMR-TVLRAEN-NLHAFCIKVLCCADCLMGVYLFFVGIFDVKFRGEYNKNAKVWMESLECRTIGFLAMLSSEVSVMLLTYLTLEKFLVIVFPFSHLRPSKCQTFTVLV-SIWLLGISIAAVPLLNEDTFGNYY-------GLNGVCFPLHSERLEKPVAKGYSTGIFLGLNLLAFLIIVVSYSSMFYSIYKTGIRATELPGR--LHRDVAMAHRFFFIVFSDALCWIPIFMVKILSLL-EVEIPGTITSWVVIFILPINSALNPILYTLTT 690
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Zebrafish
Match: rxfp1 (relaxin family peptide receptor 1 [Source:ZFIN;Acc:ZDB-GENE-110125-1]) HSP 1 Score: 142.124 bits (357), Expect = 9.711e-34 Identity = 177/685 (25.84%), Postives = 301/685 (43.94%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITH---------------IELPSMKLYEI----------------NDYTFLGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTLVINVNK---------------NDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISI----------ALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXX-XXXXXXXXXXXVTGYVLIFFHSCR--TRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL----TWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G DC G DEE+C + W H F D GI + G C + P +C+C ++ C + ++ +++ +++ + +L++ +F ++ L L + N I ++ F GL+NL L LS N I L FQ L +L+ L + +N + I S F L S+ +E+P + +I N T L + N IS +++S+N I +I D FV + +L L I+ N +++ G +PLK+LT ++Y ++ C +C +D S+ EDLLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I + D +R +Y ++ W S AC V G A +S + SV + +TL+++ICI++ +R + V ++ +WV+ +F++FY ++G+C S + E Q +SI++ L L+ +IV Y +F++ R T+ T + + AK+ ++++ LCWIPI IL LS LME + +W+ ++PINS++NP LYT +
Sbjct: 29 CPLGYFPCGNLSTCLPQVLHCNGVDDC--GNQADEENCG------DNNGWP--HLF-------DNYFGIPSNNLGNKSNACLLGTVPAECQCRDLELDCDGAHFKDVPMVSINVTMMSLQRNGLRKLNADMFLKYQSLQKLYLQHNRIKSVHPQAFRGLYNLTRLYLSYNRITTLLPDVFQDLHKLEWLILENNSLHHISSLTFSGLRSLVLLVLLNNALTKLDDICLEMPRLNWLDIEGNKMETVGNVTFRSCNMLTVLVLQRNRISRIHAQAFSLLRKLGELDLSSNRIEAIPPDLFVNLGDLLQLNISYNPIMNLRVDHFDKLHKLKSLSIEGIEIGNIHRRMFEPLKNLT--HIYFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLCCADGLMGVYLFMIGAYDLKFRGEYNRHAQAWMDSEACQVIGSLAMLSTEVSVLLLTYLTLEKYICIVY-PFRYLTPGRRRTVTILVVIWVLGFIIAFLPLLFKGVFRNFY-------GTNGVCFPLHSEQPETLGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILS-----LMEVEIPGTISSWVVIFILPINSALNPILYTLT 679
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Zebrafish
Match: rxfp1 (relaxin family peptide receptor 1 [Source:ZFIN;Acc:ZDB-GENE-110125-1]) HSP 1 Score: 141.739 bits (356), Expect = 1.355e-33 Identity = 177/685 (25.84%), Postives = 301/685 (43.94%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITH---------------IELPSMKLYEI----------------NDYTFLGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTLVINVNK---------------NDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISI----------ALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXX-XXXXXXXXXXXVTGYVLIFFHSCR--TRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL----TWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G DC G DEE+C + W H F D GI + G C + P +C+C ++ C + ++ +++ +++ + +L++ +F ++ L L + N I ++ F GL+NL L LS N I L FQ L +L+ L + +N + I S F L S+ +E+P + +I N T L + N IS +++S+N I +I D FV + +L L I+ N +++ G +PLK+LT ++Y ++ C +C +D S+ EDLLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I + D +R +Y ++ W S AC V G A +S + SV + +TL+++ICI++ +R + V ++ +WV+ +F++FY ++G+C S + E Q +SI++ L L+ +IV Y +F++ R T+ T + + AK+ ++++ LCWIPI IL LS LME + +W+ ++PINS++NP LYT +
Sbjct: 29 CPLGYFPCGNLSTCLPQVLHCNGVDDC--GNQADEENCG------DNNGWP--HLF-------DNYFGIPSNNLGNKSNACLLGTVPAECQCRDLELDCDGAHFKDVPMVSINVTMMSLQRNGLRKLNADMFLKYQSLQKLYLQHNRIKSVHPQAFRGLYNLTRLYLSYNRITTLLPDVFQDLHKLEWLILENNSLHHISSLTFSGLRSLVLLVLLNNALTKLDDICLEMPRLNWLDIEGNKMETVGNVTFRSCNMLTVLVLQRNRISRIHAQAFSLLRKLGELDLSSNRIEAIPPDLFVNLGDLLQLNISYNPIMNLRVDHFDKLHKLKSLSIEGIEIGNIHRRMFEPLKNLT--HIYFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLCCADGLMGVYLFMIGAYDLKFRGEYNRHAQAWMDSEACQVIGSLAMLSTEVSVLLLTYLTLEKYICIVY-PFRYLTPGRRRTVTILVVIWVLGFIIAFLPLLFKGVFRNFY-------GTNGVCFPLHSEQPETLGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILS-----LMEVEIPGTISSWVVIFILPINSALNPILYTLT 679
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Zebrafish
Match: rxfp2l (relaxin family peptide receptor 2, like [Source:ZFIN;Acc:ZDB-GENE-060531-8]) HSP 1 Score: 136.732 bits (343), Expect = 4.092e-32 Identity = 172/676 (25.44%), Postives = 301/676 (44.53%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCP-TKCKCFGYXXXXXXXXXXXXXXXXPKT-------RTLTI---------VQTHSLE-LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF-----------------QFLK--------QLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVIN---------------VNKNDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIAL----------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G +DCP+G ++E P+ F + + + + E + Y D C K C G +L ++S N++ L + R L I ++++S+E +S FS + L L +S N I L G+F L +L +LIL +N I L +F QF K +L L++ N I+++ + +F N S+T + L ++ I + TF M + LI +++S N I+ + F + NL L I+ VN ++M +PL +LT ++Y ++ C + +C +D S+ E+LLAN ++VFVW + GNI VI R +A + + T + I +L +D MG+YL+ I + D Y +Y ++ W S++C + G A +S + SV + +TL++++CI+F A + + L L F+W++ + F +FY +G+C S + E + +S + L + L F+++ Y +F+ +T +TA + + + AK+ ++ LCWIPI +L LS + + W+ ++PINS++NP LYT +
Sbjct: 26 CPLGQFPCGNLSVCLPQPQHCNGQQDCPNGA-DEENCVDNSGWPHLFDAF--------------MKTRVQESKECMLDVYPDTCHCEGRKVNCNGKNLLHVPAVSSNVTALELNSNRIESLSRDLFIRYRHLERLHLESNSIETISKRAFSGLVALRKLFLSQNKISSLKAGIFSDLSSLEWLILDENRISSLRQKSFEGLKSLFFLSLLNNSVEQFPKTSICHEMPRLNWLEMEGNIISSLWASSFKNCSSLTVLALRRNRISTIEEGTFADM-QRLIDLDVSVNQIKDLPPSLFQNLQNLKQLNISNNPLVNIYSNQFDSLVNLQSLSMEEVEIPNISIRMFRPLTNLT--HIYFKRFEFCGYSPNVRSCKPNTDGISSFENLLANIILRVFVWVVAFIICFGNIFVICLRSCIASENQHHT--MAIKSLCCADCLMGVYLLFIGAFDIKYCGEYNRHAQIWMESLSCQLIGSLAMLSTEVSVMMLTYMTLEKYLCIVFPFQHYRAGRKQTLCSLT-FIWLLGFIIAVIPFWDKQTFGNFYG-------RNGVCFPLHSDQTEKPGARCYSTAIFLGLNLLAFVMIVFSYSSMFYSVQKTAKTARQTVYDREVTIAKRFFFIVFTDALCWIPIFLLKILSLL-RVQIAGTIILWVVIFILPINSALNPILYTIT 672
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Zebrafish
Match: rxfp1 (relaxin family peptide receptor 1 [Source:ZFIN;Acc:ZDB-GENE-110125-1]) HSP 1 Score: 127.872 bits (320), Expect = 2.146e-29 Identity = 158/618 (25.57%), Postives = 277/618 (44.82%), Query Frame = 1
Query: 4138 GISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITH---------------IELPSMKLYEI----------------NDYTFLGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTLVINVNK---------------NDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISI----------ALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXX-XXXXXXXXXXXVTGYVLIFFHSCR--TRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL----TWLTAVLMPINSSINPYLYTFS 5724
GI + G C + P +C+C ++ C + ++ +++ +++ + +L++ +F ++ L L + N I ++ F GL+NL L LS N I L FQ L +L+ L + +N + I S F L S+ +E+P + +I N T L + N IS +++S+N I +I D FV + +L L I+ N +++ G +PLK+LT ++Y ++ C +C +D S+ EDLLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I + D +R +Y ++ W S AC V G A +S + SV + +TL+++ICI++ +R + V ++ +WV+ +F++FY ++G+C S + E Q +SI++ L L+ +IV Y +F++ R T+ T + + AK+ ++++ LCWIPI IL LS LME + +W+ ++PINS++NP LYT +
Sbjct: 15 GIPSNNLGNKSNACLLGTVPAECQCRDLELDCDGAHFKDVPMVSINVTMMSLQRNGLRKLNADMFLKYQSLQKLYLQHNRIKSVHPQAFRGLYNLTRLYLSYNRITTLLPDVFQDLHKLEWLILENNSLHHISSLTFSGLRSLVLLVLLNNALTKLDDICLEMPRLNWLDIEGNKMETVGNVTFRSCNMLTVLVLQRNRISRIHAQAFSLLRKLGELDLSSNRIEAIPPDLFVNLGDLLQLNISYNPIMNLRVDHFDKLHKLKSLSIEGIEIGNIHRRMFEPLKNLT--HIYFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLCCADGLMGVYLFMIGAYDLKFRGEYNRHAQAWMDSEACQVIGSLAMLSTEVSVLLLTYLTLEKYICIVY-PFRYLTPGRRRTVTILVVIWVLGFIIAFLPLLFKGVFRNFY-------GTNGVCFPLHSEQPETLGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILS-----LMEVEIPGTISSWVVIFILPINSALNPILYTLT 615
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Xenopus
Match: RXFP1 (relaxin family peptide receptor 1 [Source:NCBI gene;Acc:100494807]) HSP 1 Score: 142.124 bits (357), Expect = 1.212e-33 Identity = 166/634 (26.18%), Postives = 286/634 (45.11%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK---------------NDIAMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLV-------FLVWFVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRI--LMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G +C G DE++C + WS L +++ + + D E C + PT+C C G ++ C + + ++P +TI++ SL+ + + YL + NI S+ F GL+NL L LS N I L F+ L +L+ L I +N IT I F L S+ + + + L + D + L ++ N+++S++ +F+ L+ L I+ N +++ G PLK+LT ++Y ++ C C +D S+ E+LLA+ +VFVW + + GNI VI R + + K I ++L +D MG+YL +I D YR +Y EN+ W S C + G A +S + SV + +TL+++ICI+ FR + + + F++ F+ + + F ++Y ++G+C S + E + Q +S+++ L + L F+I+ Y +F+ RT A I + + AK+ ++ LCWIPI IL LS + S +W+ ++PINS++NP LYT +
Sbjct: 43 CPLGYFPCGNITKCLPQFMHCNGVDEC--GNQADEDNCG------DNNGWSQQLDKLFEKHYEKIGLNVFDLEMKPSE--CTLGPVPTQCLCRGLELDCDGAKLRTVPSVSPN---VTIMKCTSLDFTCS----YRYLQHNNIRTVSV-----HAFKGLYNLTKLYLSHNEITTLKPGVFEDLHRLEWLIIENNKITRIYPQTFQGLNSLILLVMMNNFLERLPDKSLCQHMPKLNWLDFEGNNVQSLTNTTFISCATLTVLNISHNPIQKIQADQFDYVIKLKSLSLEGIEIPNIQRRMFMPLKNLT--HIYFKKFQYCGFAPHVRNCKPNTDGISSLENLLASIVQRVFVWVVSVITCFGNIFVICTRPYIRSENKLHAMSI--ISLCCADCLMGVYLFVIGYFDLKYRGEYNENAQAWMDSTQCRLVGSLAILSTEVSVLLLTYLTLEKYICIVYPFRCLKPGKCRTITTLILIWIIGFVIAFIPLSNQTFFHNYY-------GTNGVCFPLHSEQPESTAAQIYSVIIFLGVNLAAFIIIVFSYSSMFYSIHRTAIMATEIHNHIKKEMTLAKRFFFIVFTDALCWIPIFILKLLSLLQVEIPGSIS-SWVVIFILPINSALNPILYTIT 642
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Xenopus
Match: RXFP2 (relaxin family peptide receptor 2 [Source:NCBI gene;Acc:100491657]) HSP 1 Score: 131.339 bits (329), Expect = 2.535e-30 Identity = 155/596 (26.01%), Postives = 267/596 (44.80%), Query Frame = 1
Query: 4108 LCLNHKDLCNGISDCSEGEDEQY--------------------------CDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIE--------LPSMKLYEINDYTFL-GMSENLI------SINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIA----MSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLV--WFV-WVISIALF--QHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLN-----DYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSN 5727
+CL C+G+ DC G DE+Y C + + P C+C ++ C + ++ L + I S L +L N + + F GL++L+ L LS N I L F L++L+ L + +NPI I F L S+ + LP ++ Y L GM+ + +I++SNN I + SF + +L L ++ K +I +P+K+L+ ++Y ++ C C+ +D S+ E+LLAN ++VFVW + GNI VI R + + K T I + L +D MGIYL I D YR QY + ++ W S+ C G+ A +S + SV + +TL++++ I+F K + L+ L+ W V ++I+I F + F+ + + +G+C + + E + Q +S+ + L + L F+I+ Y+ +F C ++TA R +N D A + ++ +CWIP+ +L LS F + +W+ ++PINS++NP LYT +
Sbjct: 43 ICLPRTFHCDGVKDCENGADEEYCGDNSGWANIFYEVHGKTNDVIEPQECCLSRFPESCECKDTELECVDVNLEAVPYVSSNVTLLCKYNILINSFKNCISSTVFVLRFLQ--HNCLRNISQKAFFGLYHLQRLYLSNNCISYLQQGIFSHLRELKWLILDENPIIRISQDIFAGLTSLFFLSMINNSLEALPQRLCAQMLHYVSLAGMTMLEVHTLFFHAIDLSNNLITRVPLSSFKELKHLQKL--DLEKIEIPNISTRMFQPMKNLS--HIYFKKFRYCSYAPHVRVCTPLTDGISSFENLLANTILRVFVWVIACITCFGNIFVIGMRSCIQSENKTHTMSIKV--LCCADCLMGIYLFFIGVFDVKYRGQYKKYALLWMESLQCRSLGFLAMLSTEVSVLLLTFLTLEKYLAIVFPFSNIRPGKRQTLIILISLWAVGFIIAIVPFWNEDFFGNYYGK---NGVCFPLYPDQTEEAGGQGYSLGVFLGVNLLAFIIIVFSYISMF---CSIQKTALRTSEVNSHIHTDVAVANRFFFIVFSDAVCWIPVFLLKILSLF-RVEIPGTVTSWIVIFILPINSALNPILYTLTT 623
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Xenopus
Match: RXFP2 (relaxin family peptide receptor 2 [Source:NCBI gene;Acc:100491657]) HSP 1 Score: 131.339 bits (329), Expect = 2.630e-30 Identity = 158/610 (25.90%), Postives = 273/610 (44.75%), Query Frame = 1
Query: 4108 LCLNHKDLCNGISDCSEGEDEQYC-------DIF--------------QC-----PTKCKCFGYXX------XXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLIS-INMSNNDIRSISQDSFVGVNNLSTL---------------------VINVNKNDIAMSG------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLV--WFV-WVISIALF--QHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLN-----DYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
+CL C+G+ DC G DE+YC +IF +C P C+C ++ L + L P+ + ++ + +SS +F L +L N + + F GL++L+ L LS N I L F L++L+ L + +NPI I F L S+ + + + L + M LI+ ++ N I+++ SFV N L+ L VI + I + +P+K+L+ ++Y ++ C C+ +D S+ E+LLAN ++VFVW + GNI VI R + + K T I + L +D MGIYL I D YR QY + ++ W S+ C G+ A +S + SV + +TL++++ I+F K + L+ L+ W V ++I+I F + F+ + + +G+C + + E + Q +S+ + L + L F+I+ Y+ +F C ++TA R +N D A + ++ +CWIP+ +L LS F + +W+ ++PINS++NP LYT +
Sbjct: 43 ICLPRTFHCDGVKDCENGADEEYCGDNSGWANIFYEVHGKTNDVIEPQECCLSRFPESCECKDTELECVDVNLEAVPYVSSNVTLLPRCKYNILINSFKNCISSTVF----VLRFLQ--HNCLRNISQKAFFGLYHLQRLYLSNNCISYLQQGIFSHLRELKWLILDENPIIRISQDIFAGLTSLFFLSMINNSLEALPQRLCAQMP--LINWMDFEGNRIKTLESSSFVTCNELTRLSDYFCCYTIRMMLFNTKCKIDVIFRDLEKIEIPNISTRMFQPMKNLS--HIYFKKFRYCSYAPHVRVCTPLTDGISSFENLLANTILRVFVWVIACITCFGNIFVIGMRSCIQSENKTHTMSIKV--LCCADCLMGIYLFFIGVFDVKYRGQYKKYALLWMESLQCRSLGFLAMLSTEVSVLLLTFLTLEKYLAIVFPFSNIRPGKRQTLIILISLWAVGFIIAIVPFWNEDFFGNYYGK---NGVCFPLYPDQTEEAGGQGYSLGVFLGVNLLAFIIIVFSYISMF---CSIQKTALRTSEVNSHIHTDVAVANRFFFIVFSDAVCWIPVFLLKILSLF-RVEIPGTVTSWIVIFILPINSALNPILYTLT 633
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Xenopus
Match: FSHR (follicle stimulating hormone receptor [Source:HGNC Symbol;Acc:HGNC:3969]) HSP 1 Score: 103.605 bits (257), Expect = 1.544e-21 Identity = 90/351 (25.64%), Postives = 157/351 (44.73%), Query Frame = 1
Query: 4357 LNISDNSILELYNGLFDGLFNLRYLILSKN-YIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHI------ELPSMKLYE----------------------INDY------TFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVIS 5304
L ++ N I E+ N F+G NL LILS N + LP F +LDI++ I+++ SF + + +LP+++ +E I D+ T+ E+L S+N RS D + N +T + ++DIA Y C + CS D + CED++ +++V +W + I + GN +V+I I + + KF+ L+ NLA +D+ MG+YL++IA+ D +SQY +I W++ C AG+F +++ SV + +ITL+R+ I + + ++ ++ WV S
Sbjct: 174 LRLNKNGIEEIQNYAFNGT-NLNELILSGNERLARLPDLVFHGSTGPTVLDISNTRISSLPSFGLEKIKRLKAKSAYNLKKLPALEKFEELIEADLTYPSHCCAFANWKREITDWDPICNKTYESQPEDLKSLNK-----RSAQVDPILS-NGETTYDLIDKEDDIAFD----------------YFFCYGKRDF-VCSPTPDEFNPCEDIMGYTALRVLIWCVNILAITGNSVVLI--ILLTSQYKFTVPRFLMCNLAFADLCMGVYLLLIAAVDIKTKSQYYNYAIDWQTGAGCHAAGFFTVFASELSVFTLTVITLERWHTITYAMQLDRKVRFRHATMIMASGWVFS 498
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Xenopus
Match: casq1 (calsequestrin 1 [Source:Xenbase;Acc:XB-GENE-5753698]) HSP 1 Score: 96.6709 bits (239), Expect = 1.956e-19 Identity = 52/184 (28.26%), Postives = 100/184 (54.35%), Query Frame = 1
Query: 4759 GKPLKHLTVKN--VYTNVYKIC-CSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVI 5301
G+ +K+ +N + N Y+I C + C+ + D + CED++ +++ VW + + ++GN+ V+ I + K + L+ NLA +D MGIYL++IAS D + RS+Y +I W++ C+ AG+F +++ SV + +ITL+R+ I F ++++ F++ W +
Sbjct: 267 GQEIKNAQGENTPAFENHYEIIICGGSEELECTPEPDEFNPCEDIMGYNFLRIVVWFVNLLAILGNVFVLF--ILITSHYKLTVPRFLMCNLAFADFCMGIYLLLIASVDVHTRSEYYNYAINWQTGPGCNAAGFFTVFASELSVYTLTMITLERWYAITFAMRLDRKIRLRHASFIMLGGWTL 448
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Mouse
Match: Rxfp2 (relaxin/insulin-like family peptide receptor 2 [Source:MGI Symbol;Acc:MGI:2153463]) HSP 1 Score: 134.42 bits (337), Expect = 2.213e-31 Identity = 160/664 (24.10%), Postives = 281/664 (42.32%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTL------------------------------VINVNKNDIAM------------------SG--KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFL--VWFVWVISIAL-------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXX-XXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL-------TWLTAVLMPINSSINPYLYTFSN 5727
CP G F C + C+ + CDG DC G DE++C + W+ + H ++ N ++ E C + Q P C C ++ C + + ++ L++ + L +FS++ L + + N I + F GL NL+ L LS N I L F+ L QL L + DNPIT I +F L S+ + + +L + + T L ++++NN I+ I+ +F+ ++L+ L ++ V+KN +G +P+K+L+ ++Y ++ C + C +D S+ EDLLAN ++V VW + VGN +VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ AT+S + SV + +TL++F+ I+F K + V L +W V + A+ F +FY +G+C + E + +S+ + L + LL +IV YV +F C +TA L + +S + + + ++ F ++ Q+ +W+ +P+NS++NP LYT +
Sbjct: 28 CPKGYFPCGNLTKCLPRAFHCDGVDDC--GNGADEDNCG------DTSGWTT----IFGTVHGNV-NKVTLTQE------CFLSQYPQHCYCRENELECVKADLKAVPKVSSNVTLLSLKKNKIHRLPVKVFSRYTELRKIYLQHNCITHISRRAFLGLHNLQILYLSHNCITSLRPGIFKDLHQLAWLILDDNPITRISQKSFMGLNSLFFLSMVGNRLEALPE-TLCAQMPQLNWVDLANNGIKYITNSTFLTCDSLTVLDLSSNMITKLPVHLFSDLHLLQKLNLSSNPLLYVHKNQFGSLKQLQSLDLERIEIPNISTGMFQPMKNLS--HIYLKTFRYCSYVPHVRICMPSTDGISSSEDLLANGILRVSVWVIAFITCVGNFLVIAVRSLI--KAENTTHAMSIKILCCADCLMGVYLFSVGVFDIKYRGQYQKYALLWMESVPCRLLGFLATLSTEVSVLLLTFLTLEKFLVIVFPFSNLRLGKRQTAVALASIWVVGFLIAAVPFTREDYFGNFY-------GKNGVCFPLHYDQAEDFGSRGYSLGIFLGVNLLAFLVIVISYVTMF---CSIHKTA---LQTAEVRSHIGKEVAVANRFFFIVFSDAICWIPVFVVKILSLLQVEIPGTITSWIVVFFLPVNSALNPILYTLTT 654
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Mouse
Match: Rxfp2 (relaxin/insulin-like family peptide receptor 2 [Source:MGI Symbol;Acc:MGI:2153463]) HSP 1 Score: 128.642 bits (322), Expect = 1.915e-29 Identity = 130/513 (25.34%), Postives = 231/513 (45.03%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTF--------LGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSG--KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFL--VWFVWVISIAL-------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXX-XXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL-------TWLTAVLMPINSSINPYLYTFSN 5727
L GIF L +L + DN I + F GL +L +L + N + LP + QL +D+ +N I I + F S+T + LP ++ + + TF L +S N+I+ +N+S+N + + ++ F + L +L + + +G +P+K+L+ ++Y ++ C + C +D S+ EDLLAN ++V VW + VGN +VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ AT+S + SV + +TL++F+ I+F K + V L +W V + A+ F +FY +G+C + E + +S+ + L + LL +IV YV +F C +TA L + +S + + + ++ F ++ Q+ +W+ +P+NS++NP LYT +
Sbjct: 183 LRPGIFKDLHQLAWLILDDNPITRISQKSFMGLNSLFFLSMVGNRLEALPETLCAQMPQLNWVDLANNGIKYITNSTFLTCDSLTVLFLPRNQIGFVPEKTFSSLKNLGELDLSSNMITKLPVHLFSDLHLLQKLNLSSNPLLYVHKNQFGSLKQLQSLDLERIEIPNISTGMFQPMKNLS--HIYLKTFRYCSYVPHVRICMPSTDGISSSEDLLANGILRVSVWVIAFITCVGNFLVIAVRSLI--KAENTTHAMSIKILCCADCLMGVYLFSVGVFDIKYRGQYQKYALLWMESVPCRLLGFLATLSTEVSVLLLTFLTLEKFLVIVFPFSNLRLGKRQTAVALASIWVVGFLIAAVPFTREDYFGNFY-------GKNGVCFPLHYDQAEDFGSRGYSLGIFLGVNLLAFLVIVISYVTMF---CSIHKTA---LQTAEVRSHIGKEVAVANRFFFIVFSDAICWIPVFVVKILSLLQVEIPGTITSWIVVFFLPVNSALNPILYTLTT 678 HSP 2 Score: 61.2326 bits (147), Expect = 1.297e-8 Identity = 59/236 (25.00%), Postives = 100/236 (42.37%), Query Frame = 1
Query: 4111 CLNHKDLCNGISDCSEGEDE--------------------------QYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK 4740
CL C+G+ DC G DE Q C + Q P C C ++ C + + ++ L++ + L +FS++ L + + N I + F GL NL+ L LS N I L F+ L QL L + DNPIT I +F L S+ + + +L + + T L ++++NN I+ I+ +F+ ++L+ L + N+
Sbjct: 41 CLPRAFHCDGVDDCGNGADEDNCGDTSGWTTIFGTVHGNVNKVTLTQECFLSQYPQHCYCRENELECVKADLKAVPKVSSNVTLLSLKKNKIHRLPVKVFSRYTELRKIYLQHNCITHISRRAFLGLHNLQILYLSHNCITSLRPGIFKDLHQLAWLILDDNPITRISQKSFMGLNSLFFLSMVGNRLEALPE-TLCAQMPQLNWVDLANNGIKYITNSTFLTCDSLTVLFLPRNQ 275
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Mouse
Match: Rxfp2 (relaxin/insulin-like family peptide receptor 2 [Source:MGI Symbol;Acc:MGI:2153463]) HSP 1 Score: 123.635 bits (309), Expect = 5.861e-28 Identity = 128/504 (25.40%), Postives = 227/504 (45.04%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNIS--------------DNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSG--KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFL--VWFVWVISIAL-------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXX-XXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL-------TWLTAVLMPINSSINPYLYTFSN 5727
L GIF L +LNI DN I + F GL +L +L + N + LP + QL +D+ +N I I + F S+T ++L S + ++ + F + L +N+S+N + + ++ F + L +L + + +G +P+K+L+ ++Y ++ C + C +D S+ EDLLAN ++V VW + VGN +VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ AT+S + SV + +TL++F+ I+F K + V L +W V + A+ F +FY +G+C + E + +S+ + L + LL +IV YV +F C +TA L + +S + + + ++ F ++ Q+ +W+ +P+NS++NP LYT +
Sbjct: 183 LRPGIFKDLHQLAWLNIPNIGNILMCFVRILDDNPITRISQKSFMGLNSLFFLSMVGNRLEALPETLCAQMPQLNWVDLANNGIKYITNSTFLTCDSLTVLDLSSNMITKLPVHLFSDL-HLLQKLNLSSNPLLYVHKNQFGSLKQLQSLDLERIEIPNISTGMFQPMKNLS--HIYLKTFRYCSYVPHVRICMPSTDGISSSEDLLANGILRVSVWVIAFITCVGNFLVIAVRSLI--KAENTTHAMSIKILCCADCLMGVYLFSVGVFDIKYRGQYQKYALLWMESVPCRLLGFLATLSTEVSVLLLTFLTLEKFLVIVFPFSNLRLGKRQTAVALASIWVVGFLIAAVPFTREDYFGNFY-------GKNGVCFPLHYDQAEDFGSRGYSLGIFLGVNLLAFLVIVISYVTMF---CSIHKTA---LQTAEVRSHIGKEVAVANRFFFIVFSDAICWIPVFVVKILSLLQVEIPGTITSWIVVFFLPVNSALNPILYTLTT 668
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Mouse
Match: Rxfp1 (relaxin/insulin-like family peptide receptor 1 [Source:MGI Symbol;Acc:MGI:2682211]) HSP 1 Score: 118.627 bits (296), Expect = 2.652e-26 Identity = 126/474 (26.58%), Postives = 220/474 (46.41%), Query Frame = 1
Query: 4348 LGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIEL---PSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVWFV-WVISIA------LFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT---GYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
L +L+ N I L N F NL L++ KN I L +AF L++L LD+ N I + F +L ++ + + P K+ E+N + L L S+++ +I +I Q F + NLS ++Y ++ C +C +D S+ E+LLA+ +VFVW + GNI VI R + + K I++L +D MG+YL +I + D +R +Y +++ W SV C G A +S + SV + +TL+++ICI+ FR R + ++ +W + ++++ A F+++Y ++G+C S + Q +S+++ L I L F+I+ G + H T + + + AK+ ++ LCWIPI IL FLS + +S +W+ ++PINS++NP +YT +
Sbjct: 249 LHWLDFEGNRIHNLRNLTFISCNNLTVLVMRKNKINYLNEHAFTHLQKLDELDLGSNKIENLPPNIFKDLKELSQLNISYNPIQKI-EVNQFDCLA---KLKSLSLEGIEISNIQQRMFRPLINLS------------------------HIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAITCFGNIFVICMRPYIRSENKLHAMS--IISLCCADCLMGVYLFVIGAFDLKFRGEYNKHAQPWMESVHCQFMGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCLRPRKCRTITVLIFIWIIGFIVAFAPLGNKEFFKNYY-------GTNGVCFPLHSEDTGSTGAQIYSVVIFLGINLVAFIIIVFSYGSMFYSVHQSSVTVTEIQKQVKKEVVLAKRFFFIVFTDALCWIPIFILKFLSLLQVEIPDSIT-SWVVIFILPINSALNPIIYTLT 684
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Mouse
Match: Tshr (thyroid stimulating hormone receptor [Source:MGI Symbol;Acc:MGI:98849]) HSP 1 Score: 97.0561 bits (240), Expect = 1.267e-19 Identity = 48/152 (31.58%), Postives = 88/152 (57.89%), Query Frame = 1
Query: 4843 CSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWV 5298
C+ KSD + CED++ + +++ VW + + L+GNI V++ I + K + L+ NLA +D MG+YL++IAS D Y S+Y ++I W++ C+ AG+F +++ SV + +ITL+R+ I F ++++ ++ WV
Sbjct: 398 CTPKSDEFNPCEDIMGYRFLRIVVWFVSLLALLGNIFVLL--ILLTSHYKLTVPRFLMCNLAFADFCMGVYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHAYTIMAGGWV 547
BLAST of G-protein coupled receptor GRL101 vs. UniProt/SwissProt
Match: sp|P46023|GR101_LYMST (G-protein coupled receptor GRL101 OS=Lymnaea stagnalis OX=6523 PE=2 SV=1) HSP 1 Score: 247.669 bits (631), Expect = 1.982e-65 Identity = 249/891 (27.95%), Postives = 399/891 (44.78%), Query Frame = 1
Query: 3304 FWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSR--CAELTEWQCQN----KQCIDVSKRCNKVSDCIDN---SDEKDCD---ICNGFK-------CLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIK-SLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWS-------------------FNHSFLL------------------CLNHKDLCNGISDCSEGE-DEQYCDIFQCPTK---CKCFGYXXX-XXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIY--LGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVK---------NVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQH---------ASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSNV 5730
F C E I + VCD +DC ++ DE +C K ++F+ +D + I + CD DCK DE++C+ + C T++ C++ +CID CN ++DC D +DE C C F+ C C+A D DC E ++ C ++ + C S +C S + C+ S+ + NC + QC + +CIE H C+ ++CPDG DE + + + FRC + + S L+ C+N +C+G DC +G DE C + CP C+C G + L + + ++ + L L+S FS Y + YL++S N + E+ F ++ L +L L+ N I L + + L L+ L I N I TIE F ++I +T ++L + +L + F G+ + + +N+S N I SI +F NNL+ + + I +SG +K + K + T+ Y+ CC CS K D S+CEDL++N ++V +W +G+ LVGN +VI WR+ R K + LI NLAI D MG+YL+IIA+AD YYR YI + WK S C AG+ +T S++ SV + ITLDR ICI+F R S I LVFL+ + ++ + F++FY SG+CL+ + + +L LL LI + Y+ +F + +TR ND A+++ +++ CW+PII+L F+S + + W+ ++P+NS+ NP +YT S
Sbjct: 200 FQCAE--GSLIKKEFVCDGWVDCKLTFADELNC----KLCDEDDFRC------SDTRCIQKSNVCDGYCDCKTCDDEEVCANNTYGCPMDTKYMCRSIYGEPRCIDKDNVCNMINDCRDGNVGTDEYYCSNDSECKNFQAAMGFFYCPEERCLAKHLYCDLHPDCINGEDEQSCLAPPKCSQDEFQCHHGKCIPISKRC-DSVHDCVDWSDEM-------------NCENHQCAANMKSCLSGHCIEEHKWCNFHRECPDGSDEKDCDPRPVCEANQFRCKNGQCIDPLQVCVKGDKYDGCADQSHLINCSQHICLEGQFRCRKSFCINQTKVCDGTVDCLQGMWDENNCR-YWCPHGQAICQCEGVTMDCTGQKLKEMPVQQMEEDLSKLMIGDNLLNLTSTTFSATYYDKVTYLDLSRNHLTEIPIYSFQNMWKLTHLNLADNNITSLKNGSLLGLSNLKQLHINGNKIETIEEDTFSSMIHLTVLDLSNQRLTHVYKNMFKGLKQ-ITVLNISRNQINSIDNGAF---NNLANVRL------IDLSGNVIKDIGQKVFMGLPRLVELKTDSYRFCCLAPEGVKCSPKQDEFSSCEDLMSNHVLRVSIWVLGVIALVGNFVVIFWRVRDFRGGKVHS--FLITNLAIGDFLMGVYLLIIATADTYYRGVYISHDENWKQSGLCQFAGFVSTFSSELSVLTLSTITLDRLICILFPLRRTRLGLRQAIIVMSCIWVLVFLLAVLPLLGFSYFENFYG-------RSGVCLALHVTPDRRPGWEYSVGVFILLNLLSFVLIASSYLWMFSVAKKTRSAVRTAESKNDNAMARRMTLIVMTDFCCWVPIIVLGFVS-LAGARADDQVYAWIAVFVLPLNSATNPVIYTLSTA 1043 HSP 2 Score: 79.337 bits (194), Expect = 2.930e-13 Identity = 77/239 (32.22%), Postives = 110/239 (46.03%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD----ICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNN--TCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYN--FRCWSFNHSFLLCLNHKDLCNGISDCSEGE---DEQYC 4179
CD +DC NG DE+ C C T + N C+ K+C+ + DC DNSDEK C+ + FKC +S C+A D +VDC S ED++ D Y AE S K F + C + L+ C L + F+CS + CI+ VCDG DC +DE+ + T CP + + C S + C++ ++CN I+DC +G DE YC
Sbjct: 98 CDRNDDCSNGLDERECDIYICPLGTHVKWHNHFCVPRDKQCDFLDDCGDNSDEKICERRECVATEFKCNNSQCVAFGNLCDGLVDCVDGSDEDQVACDSDKYFQCAE-----GSLIKKEFVCDGWVDCKLTFADELN---CKLCDEDD----------FRCSDTR-CIQKSNVCDGYCDCKTCDDEEVCANNTYGCPMDTKYMCRSI-YGEPRCIDKDNVCNMINDCRDGNVGTDEYYC 316 HSP 3 Score: 67.3958 bits (163), Expect = 1.654e-9 Identity = 80/311 (25.72%), Postives = 131/311 (42.12%), Query Frame = 1
Query: 3325 AYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKP-MNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAEL-TEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI-----CNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKY-------CIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYC--DIFQCPTKCK 4209
A G VC ++ ++ + + ++ S P + + C+ Q + CD + DC+NG DE S C L +E+QC + CID RC++ DC + DE++CDI K + C+ + D + DC G + +++I + +C +T KC +N+ C+ N L + L +C+ C KY I+ +VCDG DC DE +CK +FRC S C+ ++C+G DC +DE+ C + + CP K
Sbjct: 2 ATMSGTTIVCLIYLTTMLGNSQGVNLKIESPSPPTLCSVEGTFHCDDGMLQCVLMGSKCDGVSDCENGMDE---SVETCGCLQSEFQCNHTTCIDKILRCDRNDDCSNGLDERECDIYICPLGTHVKWHNHFCVPRDKQCDFLDDC-GDNSDEKICERRECV-----------ATEFKC--NNSQCVAFGN---------LCDGLVDCVDGSDEDQVACDSDKYFQCAEGSLIKKEFVCDGWVDCK-LTFADELNCKLCD-EDDFRC-----SDTRCIQKSNVCDGYCDCKTCDDEEVCANNTYGCPMDTK 279
BLAST of G-protein coupled receptor GRL101 vs. UniProt/SwissProt
Match: sp|Q91ZZ5|RXFP2_MOUSE (Relaxin receptor 2 OS=Mus musculus OX=10090 GN=Rxfp2 PE=2 SV=2) HSP 1 Score: 128.642 bits (322), Expect = 1.339e-28 Identity = 130/513 (25.34%), Postives = 231/513 (45.03%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTF--------LGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSG--KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFL--VWFVWVISIAL-------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXX-XXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL-------TWLTAVLMPINSSINPYLYTFSN 5727
L GIF L +L + DN I + F GL +L +L + N + LP + QL +D+ +N I I + F S+T + LP ++ + + TF L +S N+I+ +N+S+N + + ++ F + L +L + + +G +P+K+L+ ++Y ++ C + C +D S+ EDLLAN ++V VW + VGN +VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ AT+S + SV + +TL++F+ I+F K + V L +W V + A+ F +FY +G+C + E + +S+ + L + LL +IV YV +F C +TA L + +S + + + ++ F ++ Q+ +W+ +P+NS++NP LYT +
Sbjct: 183 LRPGIFKDLHQLAWLILDDNPITRISQKSFMGLNSLFFLSMVGNRLEALPETLCAQMPQLNWVDLANNGIKYITNSTFLTCDSLTVLFLPRNQIGFVPEKTFSSLKNLGELDLSSNMITKLPVHLFSDLHLLQKLNLSSNPLLYVHKNQFGSLKQLQSLDLERIEIPNISTGMFQPMKNLS--HIYLKTFRYCSYVPHVRICMPSTDGISSSEDLLANGILRVSVWVIAFITCVGNFLVIAVRSLI--KAENTTHAMSIKILCCADCLMGVYLFSVGVFDIKYRGQYQKYALLWMESVPCRLLGFLATLSTEVSVLLLTFLTLEKFLVIVFPFSNLRLGKRQTAVALASIWVVGFLIAAVPFTREDYFGNFY-------GKNGVCFPLHYDQAEDFGSRGYSLGIFLGVNLLAFLVIVISYVTMF---CSIHKTA---LQTAEVRSHIGKEVAVANRFFFIVFSDAICWIPVFVVKILSLLQVEIPGTITSWIVVFFLPVNSALNPILYTLTT 678 HSP 2 Score: 61.2326 bits (147), Expect = 9.073e-8 Identity = 59/236 (25.00%), Postives = 100/236 (42.37%), Query Frame = 1
Query: 4111 CLNHKDLCNGISDCSEGEDE--------------------------QYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK 4740
CL C+G+ DC G DE Q C + Q P C C ++ C + + ++ L++ + L +FS++ L + + N I + F GL NL+ L LS N I L F+ L QL L + DNPIT I +F L S+ + + +L + + T L ++++NN I+ I+ +F+ ++L+ L + N+
Sbjct: 41 CLPRAFHCDGVDDCGNGADEDNCGDTSGWTTIFGTVHGNVNKVTLTQECFLSQYPQHCYCRENELECVKADLKAVPKVSSNVTLLSLKKNKIHRLPVKVFSRYTELRKIYLQHNCITHISRRAFLGLHNLQILYLSHNCITSLRPGIFKDLHQLAWLILDDNPITRISQKSFMGLNSLFFLSMVGNRLEALPE-TLCAQMPQLNWVDLANNGIKYITNSTFLTCDSLTVLFLPRNQ 275
BLAST of G-protein coupled receptor GRL101 vs. UniProt/SwissProt
Match: sp|Q8WXD0|RXFP2_HUMAN (Relaxin receptor 2 OS=Homo sapiens OX=9606 GN=RXFP2 PE=1 SV=1) HSP 1 Score: 126.716 bits (317), Expect = 6.114e-28 Identity = 131/514 (25.49%), Postives = 241/514 (46.89%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTF--------LGMSENLIS---------------INMSNNDIRSISQDSFVGVNNLSTL------VINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIF-----RTYRQHASKIKYLVFLVWFVWVI----SIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILML-----NDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
L GIF L +L + DN I + LF GL +L +L + NY+ LP + QL +D+ N I + + F + S+T + LP ++ + + TF L +S N I+ +N+S+N + + ++ F + L +L + N+N +P+K+L+ ++Y ++ C C +D S+ EDLLAN +++FVW + GN+ VI R + K + +T + I L +D MG+YL + D YR QY + ++ W SV C + G+ A +S + SV + +TL++F+ I+F R ++ S I +++ F+ + + F +FY +G+C + + E + +S+ + L + L FLI+ Y+ +F C ++TA + + + A + ++ +CWIP+ ++ LS F + ++ +W+ +P+NS++NP LYT +
Sbjct: 200 LRPGIFKDLHQLTWLILDDNPITRISQRLFTGLNSLFFLSMVNNYLEALPKQMCAQMPQLNWVDLEGNRIKYLTNSTFLSCDSLTVLFLPRNQIGFVPEKTFSSLKNLGELDLSSNTITELSPHLFKDLKLLQKLNLSSNPLMYLHKNQFESLKQLQSLDLERIEIPNINTRMF----QPMKNLS--HIYFKNFRYCSYAPHVRICMPLTDGISSFEDLLANNILRIFVWVIAFITCFGNLFVIGMRSFI--KAENTTHAMSIKILCCADCLMGVYLFFVGIFDIKYRGQYQKYALLWMESVQCRLMGFLAMLSTEVSVLLLTYLTLEKFLVIVFPFSNIRPGKRQTSVILICIWMAGFLIAVIPFWNKDYFGNFY-------GKNGVCFPLYYDQTEDIGSKGYSLGIFLGVNLLAFLIIVFSYITMF---CSIQKTALQTTEVRNCFGREVAVANRFFFIVFSDAICWIPVFVVKILSLFRVEIPDT-MTSWIVIFFLPVNSALNPILYTLT 694
BLAST of G-protein coupled receptor GRL101 vs. UniProt/SwissProt
Match: sp|Q5XM32|RXFP2_CANLF (Relaxin receptor 2 OS=Canis lupus familiaris OX=9615 GN=RXFP2 PE=2 SV=1) HSP 1 Score: 126.331 bits (316), Expect = 6.412e-28 Identity = 129/517 (24.95%), Postives = 234/517 (45.26%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTF--------LGMSENLI---------------SINMSNNDIRSISQDSFVGVNNLSTL------VINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIAL----------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIF-------FHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
L G+F L +L + DN I + LF GL +L +L + NY+ LP + QL +D+ N I + + +F + S+T + LP ++ + + TF L +S N+I +N+S+N + + ++ F + L +L + N+N +P+ +L+ ++Y ++ C C +D S+ EDLLAN +++FVW + GN+ VI R + K + +T I L +D MG+YL I D YR QY + ++ W S+ C + G+ A +S + SV + +TL++F+ I+F K + +V L+ +W++ + F +FY +G+C + + E + +S+ + L + L FLI+ Y ++F + R GR + A + ++ +CWIP+ ++ LS F + +W+ +P+NS++NP LYT +
Sbjct: 183 LRPGVFKDLHQLTWLILDDNPITRISQQLFTGLKSLFFLSMVNNYLEALPKQMCAQMPQLNWMDLEGNGIKYLTNSSFLSCNSLTVLFLPRNQIDFVPEKTFSSLKNLGELDLSSNMIMELPPEIFKDLKLLQKLNLSSNPLLYLHKNQFESLKQLQSLDLERIEIPNINTRMF----QPMMNLS--HIYFKNFRYCSYAPHVRICMPLTDGISSFEDLLANNILRIFVWVIAFITCFGNLFVIGMRSFI--KAENTTHATSIKILCCADCLMGVYLFFIGFFDIKYRGQYQKYALLWMESLQCRLMGFLAMLSTEVSVLLLTYLTLEKFLAIVFPFSNIRPGKWQTMVILIC-IWIVGFLIAVIPFWKEDYFGNFY-------GKNGVCFPLYYDQTEDIGSKGYSLGIFLGVNLLAFLIIVFSYTIMFCSIKKTALQTSEVRNPIGR-----EVAVANRFFFIVFSDAICWIPVFVIKILSLF-RVEIPGTITSWIVIFFLPVNSALNPILYTLT 677
BLAST of G-protein coupled receptor GRL101 vs. UniProt/SwissProt
Match: sp|Q9HBX9|RXFP1_HUMAN (Relaxin receptor 1 OS=Homo sapiens OX=9606 GN=RXFP1 PE=1 SV=2) HSP 1 Score: 123.635 bits (309), Expect = 4.654e-27 Identity = 132/510 (25.88%), Postives = 229/510 (44.90%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF-QFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMS------------ENLISI-----------NMSNNDIRSISQDSF---VGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYL---MESDQLTWLTAVLMPINSSINPYLYTFS 5724
L G+F L +L I DN + + F GL +L L+L N + LP Q + +L LD+ N I + + F + ++T + + K+ +N+ TF + ENL + N+S N I+ I + F V + +LS I ++ M +PL +L+ ++Y ++ C +C +D S+ E+LLA+ +VFVW + GNI VI R + + K I++L +D MGIYL +I D +R +Y +++ W S C + G A +S + SV + +TL+++ICI+ FR R + ++ L+W F+ + + F+++Y ++G+C S E Q +S+ + L I L F+I+ Y +F+ ++ TA I N K L + I F+ +F + L + +W+ ++PINS++NP LYT +
Sbjct: 189 LKPGVFEDLHRLEWLIIEDNHLSRISPPTFYGLNSLILLVLMNNVLTRLPDKPLCQHMPRLHWLDLEGNHIHNLRNLTFISCSNLTVLVMRKNKINHLNENTFAPLQKLDELDLGSNKIENLPPLIFKDLKELSQLNLSYNPIQKIQANQFDYLVKLKSLSLEGIEISNIQQRMF-RPLMNLS--HIYFKKFQYCGYAPHVRSCKPNTDGISSLENLLASIIQRVFVWVVSAVTCFGNIFVICMRPYIRSENKLYAMS--IISLCCADCLMGIYLFVIGGFDLKFRGEYNKHAQLWMESTHCQLVGSLAILSTEVSVLLLTFLTLEKYICIVYPFRCVRPGKCRTITVLILIWITGFIVAFIPLSNKEFFKNYY-------GTNGVCFPLHSEDTESIGAQIYSVAIFLGINLAAFIIIVFSYGSMFYSVHQSAITATEI--RNQVKKEMILAKRFFFIVFTDALCWIPIFVVKFLSLLQVEIPGTITSWVVIFILPINSALNPILYTLT 684 HSP 2 Score: 55.4546 bits (132), Expect = 5.369e-6 Identity = 63/265 (23.77%), Postives = 106/265 (40.00%), Query Frame = 1
Query: 3946 GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK 4740
G F C + C+ C+G DC + DED C N WS +K + E C + P +C C G ++ C + + ++ +++ +L F + L L + +N I + F GL +L L LS N I L F+ L +L+ L I DN ++ I F+ L S+ + L + L + D L +++ N I ++ +F+ +NL+ LV+ NK
Sbjct: 30 GYFPCGNITKCLPQLLHCNGVDDCGNQADED-------NCGDN-NGWSLQFDKYFASYYKMTSQYPFEAETPE----CLVGSVPVQCLCQGLELDCDETNLRAVPSVSSNVTAMSLQWNLIRKLPPDCFKNYHDLQKLYLQNNKITSISIYAFRGLNSLTKLYLSHNRITFLKPGVFEDLHRLEWLIIEDNHLSRISPPTFYGLNSLILLVLMNNVLTRLPDKPLCQHMPRLHWLDLEGNHIHNLRNLTFISCSNLTVLVMRKNK 282
BLAST of G-protein coupled receptor GRL101 vs. TrEMBL
Match: A0A267GIT1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009194g3 PE=4 SV=1) HSP 1 Score: 397.127 bits (1019), Expect = 2.628e-109 Identity = 293/875 (33.49%), Postives = 438/875 (50.06%), Query Frame = 1
Query: 3229 RVCICERSSQTIKSLINASKEQI----NGFWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDN--QFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQN-KQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIIS---KEDCYK-----NPAEIK---------SLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCI----SFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFN---HSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXX-XXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFV-GVNNLSTLVINVNKNDIAMSGKPLKHLT-VKNVYTNVYKICCSLASYGT-CSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGL--CLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTG-YVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSNVNLF 5739
R +CERS EQ+ N F C +D I D CD DC E DE DC +V + F CN Q ISW+R CD DC G DE C +S C + E+QC+ K+CI +RCN +S C D +DE++C+ CNGF+CLS CI + D VDCPGS +EDE ++ +C + +P K C KCF + C++ + CP +EHL++C+ F C LFQC YCI ++ +CDG +DC DG DE + S L + RC S + SF + + ++ C+GI+ C +GEDE +CD+ +C C C GY + ++P TR L + + G F LG L + + E+ GLF L NLRYL LS N I I+P + + L L L I NPI TIESF F +L + ++L +M + + +Y FL S + +N+S N ++SI + +F G + L+TL ++ D+ + L+ + N+YT+ +K CC C+ +D S+C+DLL + + QV VW G L GN+ VI WR+ KK T D LI+NL+++D+ MG+YL+IIA D YR YI + W++S C+V AT+S++ SVT + ++ DR+ + R ++QH ++ + +V W+ + + F + L S F + Q + + F +TG Y+ +F + TRR A R M D+ A++L L+L ++CW+P I+L FL S Y + T L +PINS++NPYLYT S + +F
Sbjct: 654 RSVLCERS---------PVAEQVGCRSNQFAC--LDGTCISSDGQCDGKKDCPSGE-DEMDCSSEVVDR-------FFECNSGGTHLQRISWSRLCDHKVDCSTGLDEADCQFSPC-KANEFQCERTKECIPNEQRCNGISSCQDLTDEQNCETCNGFQCLSGRCIPNGLVNDTQVDCPGSLLEDEAMTCLRANNCTRGEDVIDPGSRKFPDCPDSQWERCMLNHVKCFPRRDACVFDRRQ-FGKQVCPNMEHLRSCVGDRAGFGCRRLFQCPS-GYCIPVYRLCDGVQDCSDGADEIDCSEPPLLIGH-LRCRSPHGVLGSFNISVPAENSCDGIAHCRDGEDEAFCDM-ECRQPCNCRGYITECLRPLNFNINQTVSPLTRALYLSGNTQVSAKPGDFDHLSLLGLLEMRSIGLKEIAVGLFFYLVNLRYLDLSNNEISIIPGHVWAGLVHLNTLLIRGNPIVTIESFGFAHLPLVVDLDLSNMSIASLPNY-FLFDSNSTRHLNLSRNPLKSIEEMAFTGGPHQLNTLDLSGTGQDVYIPIGTFSKLSRLDNLYTDSFKFCCLADGIAVNCTPPADGFSSCDDLLGDLAQQVMVWIFGCLALFGNLFVIAWRVVKKLLKK--TTDFLILNLSLADLLMGVYLLIIAGTDTSYRHIYIAHDDQWRASSLCTVCAILATISSEVSVTILAFLSYDRYKLTVQR-FKQHGWTLQRVAIIVGSTWLATCSFSLLFLSETVRRNMPYNLNAACSPFYLDSQIPMLQRVPQFVAIVYNCAAFAFMTGCYLRVFLYLKNTRRKAKRKAMDIDHLMARRLFLLVLTDLICWLPTIVL-FLLVSSGYKISRTTTTGLVIFALPINSALNPYLYTVSRLRMF 1499 HSP 2 Score: 145.976 bits (367), Expect = 5.102e-31 Identity = 113/436 (25.92%), Postives = 193/436 (44.27%), Query Frame = 2
Query: 1850 NYLMLLEINSNCSYIPHNWSIHLELASRRRIECNSVNNIPSGIISLNSSTLSNVTYLDLVIPICTWHFKRPNYPTVYNSVNMLLL---------------------EIDVFNLD-CKKYTFHMFEKIGDTVHKLNLCDNVKHGDLIYSKSDGDIXXXXXXXXXXXXWNDLVPQYHFDDLVIFFKY----VSVNFTPVKMNCEDGWWKYDKSCYKFEKRQLDISWNDSLEICKTYNSKLVQISNRNEMEFLRNISLSRWLSEIYSNTSVVIFIGLRNH----------ITMPK-DYDYVEDLYRLT----WTKDEPLTYHEWFQRSPSKTYAFLSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPLYWSNWVKIPCESKISSASFICQKPQ---YIKNSKYYFTKELEKINDMDGARCSENWFFIEKRCLMLIKIN 3025
+YL+ + S C + P +WSIHLE R R+ N + N+ N +V C + + PNYP Y + L+ +DVF LD C M E I + K K G+ + S + L ++ +S + + + I FK + F P E GW ++ SCY +++ +SW+++ C LV I +++EM ++ + L W SE +++ V I+IGLR + +P+ D + D + L+ W+ EP +HEWF Y+ S +++ IN QP+ + N + C MM+ +P + SNW+K+PC +I S IC++ + + + +F ++LEKI + C++NW E++C ML+ +N
Sbjct: 132 SYLVTVSYTSTCPFSPDHWSIHLEQKCRTRVPHNEIKNV-------------NSDSEQIVQHECGF-LETPNYPFAYQNYEEPLVWIFDPLQSRNESESLSHYYEFSLDVFTLDVCLNSKLLMSESINGSFSKETTLCGYKKGEKFSTISSRRLLLMFLASKISQTSSS------YQGFSISFKLKQDASRMKFEPCS---EPGWISFNNSCYAYKRPSAPVSWDEASMECSRLGGHLVSIQSQDEMNAIKQMLLYEWHSEAFAHPDVFIYIGLRARPEVRTEFRPTVRIPQEDESFNMDYFYLSNNFEWSNGEPFVFHEWFPSHYETVYSRSSPNVQAFINEEFPQPSNDPN-YPCVGMMLHNPRHHSNWIKVPCNIEIKGQSHICERSRDDNHKVELERHFNQQLEKIR-AEAPTCADNWIEHERQCYMLLTLN 542
BLAST of G-protein coupled receptor GRL101 vs. TrEMBL
Match: A0A267E6X1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009194g2 PE=4 SV=1) HSP 1 Score: 396.356 bits (1017), Expect = 7.053e-108 Identity = 294/875 (33.60%), Postives = 438/875 (50.06%), Query Frame = 1
Query: 3229 RVCICERSSQTIKSLINASKEQI----NGFWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDN--QFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQN-KQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIIS---KEDCYK-----NPAEIK---------SLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCI----SFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFN---HSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXX-XXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFV-GVNNLSTLVINVNKNDIAMS-GKPLKHLTVKNVYTNVYKICCSLASYGT-CSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGL--CLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTG-YVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSNVNLF 5739
R +CERS EQ+ N F C +D I D CD DC E DE DC +V +F CN Q ISW+R CD DC G DE C +S C + E+QC+ K+CI +RCN +S C D +DE++C+ CNGF+CLS CI + D VDCPGS +EDE ++ +C + +P K C KCF + C++ + CP +EHL++C+ F C LFQC YCI ++ +CDG +DC DG DE + S L + RC S + SF + + ++ C+GI+ C +GEDE +CD+ +C C C GY + ++P TR L + + G F LG L + + E+ GLF L NLRYL LS N I I+P + + L L L I NPI TIESF F +L + ++L +M + + +Y FL S + +N+S N ++SI + +F G + L+TL ++ D+ + G K + N+YT+ +K CC C+ +D S+C+DLL + + QV VW G L GN+ VI WR+ KK T D LI+NL+++D+ MG+YL+IIA D YR YI + W++S C+V AT+S++ SVT + ++ DR+ + R ++QH ++ + +V W+ + + F + L S F + Q + + F +TG Y+ +F + TRR A R M D+ A++L L+L ++CW+P I+L FL S Y + T L +PINS++NPYLYT S + +F
Sbjct: 1221 RSVLCERS---------PVAEQVGCRSNQFAC--LDGTCISSDGQCDGKKDCPSGE-DEMDCSSEV-------VDRFFECNSGGTHLQRISWSRLCDHKVDCSTGLDEADCQFSPC-KANEFQCERTKECIPNEQRCNGISSCQDLTDEQNCETCNGFQCLSGRCIPNGLVNDTQVDCPGSLLEDEAMTCLRANNCTRGEDVIDPGSQKFPDCPDSQWERCMLNHVKCFPRRDACVFDRRQ-FGKQVCPNMEHLRSCVGDRAGFGCRRLFQCPS-GYCIPVYRLCDGVQDCSDGADEIDCSEPPLLTGH-LRCHSPHGVLGSFNISVPAENSCDGIAHCRDGEDEAFCDM-ECRQPCNCRGYITECLRPLNFNINQTVSPLTRALYLSGNTQVSAKPGDFDHLSLLGLLEMRSIGLKEIAVGLFFYLVNLRYLDLSNNEISIIPGHVWAGLVHLNTLLIRGNPIVTIESFGFAHLPLVVDLDLSNMSIASLPNY-FLFDSNSTRHLNLSRNPLKSIDEMAFTGGPHQLNTLDLSGTGQDVYIPIGTFAKLSRLDNLYTDSFKFCCLADGIAVNCTPPADGFSSCDDLLGDLAQQVMVWIFGCLALFGNLFVIAWRVVKKLLKK--TTDFLILNLSLADLLMGVYLLIIAGTDTSYRHIYIAHDDQWRASSLCTVCAILATISSEVSVTILAFLSYDRYKLTVQR-FKQHGWTLQRVAIIVGSTWLATCSFSLLFLSETVRRNMPYNLNAACSPFYLDSQIPMLQRVPQFVAIVYNCAAFAFMTGCYLRVFLYLKNTRRKAKRKAMDIDHLMARRLFLLVLTDLICWLPTIVL-FLLVSSGYKISRTTTTGLVIFALPINSALNPYLYTVSRLRMF 2066 HSP 2 Score: 144.821 bits (364), Expect = 1.515e-30 Identity = 112/436 (25.69%), Postives = 193/436 (44.27%), Query Frame = 2
Query: 1850 NYLMLLEINSNCSYIPHNWSIHLELASRRRIECNSVNNIPSGIISLNSSTLSNVTYLDLVIPICTWHFKRPNYPTVYNSVNMLLL---------------------EIDVFNLD-CKKYTFHMFEKIGDTVHKLNLCDNVKHGDLIYSKSDGDIXXXXXXXXXXXXWNDLVPQYHFDDLVIFFKY----VSVNFTPVKMNCEDGWWKYDKSCYKFEKRQLDISWNDSLEICKTYNSKLVQISNRNEMEFLRNISLSRWLSEIYSNTSVVIFIGLRNH----------ITMPK-DYDYVEDLYRLT----WTKDEPLTYHEWFQRSPSKTYAFLSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPLYWSNWVKIPCESKISSASFICQKPQ---YIKNSKYYFTKELEKINDMDGARCSENWFFIEKRCLMLIKIN 3025
+YL+ + S C + P +WSIHLE R R+ N + N+ N +V C + + PNYP Y + L+ +DVF LD C M E I + K K G+ + S + L ++ +S + + + I FK + F P E GW ++ SCY +++ +SW+++ C LV I +++EM ++ + L W SE +++ V I+IGLR + +P+ D + D + L+ W+ EP +HEWF Y+ S +++ IN QP+ + N + C MM+ +P + SNW+K+PC +I S IC++ + + + +F ++LEKI + C++NW +++C ML+ +N
Sbjct: 699 SYLVTVSYTSTCPFSPDHWSIHLEQKCRTRVPHNEIKNV-------------NSDSEQIVQHECGF-LETPNYPFAYQNYEEPLVWIFDPLQSRNESESLSHYYEFSLDVFTLDVCLNSKLLMSESINGSFSKETTLCGYKKGEKFSTISSRRLLLMFLASKISQTSSS------YQGFSISFKLKQDASRMKFEPCS---EPGWISFNNSCYAYKRPSAPVSWDEASMECSRLGGHLVSIQSQDEMNAIKQMLLYEWHSEAFAHPDVFIYIGLRARPEVRTEFRPTVRIPQEDESFNMDYFYLSNNFEWSNGEPFVFHEWFPSHYETVYSRSSPNVQAFINEEFPQPSNDPN-YPCVGMMLHNPRHHSNWIKVPCNIEIKGQSHICERSRDENHKVELERHFNQQLEKIR-AEAPTCADNWIEHKRQCYMLLTLN 1109
BLAST of G-protein coupled receptor GRL101 vs. TrEMBL
Match: W4Y9H0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 358.992 bits (920), Expect = 2.855e-99 Identity = 260/843 (30.84%), Postives = 435/843 (51.60%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDC--------IVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCY-KNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPG-LFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFG--YXXXXXXXXXXXXXXXXPKTRTLTIV----------QTHSLELSSGIFSQFIYLGY--------LNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVN-----KNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASK--IKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTA-GRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSNVNL 5736
VCD +C+ E DEK+C I S P + FQ C+ + IS + YCD++ C++ +DE+ C+Y +C + E+QC N QCI+ S++CN + DC+D SDE+ C C+G F+C S CI D DC G + EDE + C ++ ++ L + + +S CLY + + C + HL++C +C G F+C YC+ L CD DCP GEDE E C++ CP RC H C+ +C+G+ DC +G+DE +CDI CP+ C C G + + ++ P I+ + + + +YL L + N I + NG F NL+ L LS+N I +L S F+ L QL LD++ NP+T ++ AF++L S+ + L +++L + + F+G+ I IN+++N+I ++ +F G+N TL + N + DI L+HL+ ++ + CC ++ +C D S+CEDL+ + ++VF+W +G+ +GNI VII R V KK F + LI NLAISD MG+Y++IIASAD +Y+S Y+ ++ WK+S C +AG A +S++ SV + +I+LDRF+CI F ++H + ++ ++W + + I F ++ + SG+CL+ + Q +++ + + + I+ Y++I+ + R+ T M +++K A + ++ I CW+PI+IL+F+S ++ ++ W+ ++PINS+INPYLYT S V++
Sbjct: 154 VCDGQEECIGGE-DEKNCGDSEFIDDITAAPSCPDSSFQ----CDMG--RCISASFYCDYVPHCQDMSDEEHCAYPQCKD-DEFQCSNGQCIEASQQCNIIPDCVDGSDEELCQFCSGETFQCYDSRCIPDRSVCDGSQDCRGLNKEDE----QSCESRDDDRLRCL----NGEKLESRRRCLYDFDEYGLQQGCRDVTHLRDCGDIECFGNTFKCPN-SYCLPLRRRCDSAADCPGGEDEFE--CESYSCPGFLRC----HGERYCVTDDQICDGVKDCPDGDDEMFCDI-ACPSGCSCDGLSFDCRSFEVWTPELAASIPTDARQIIIADVSQNSRSRRAADITWNQTTIEDVLYLSLYPYTLLLSLGLPGNGIEVIINGTFITQRNLKKLDLSRNNIWMLYSDTFRGLNQLTELDLSSNPLTVLQPGAFNSLHSLPVLRLQNLELTALEEGAFVGLDSVRI-INLADNNIERVTSGAFNGLNYTRTLNLAGNDIVTFEMDIFRGLDGLRHLS-----SDRFLFCCIVSGLESCYPPEDQFSSCEDLMRSDVLRVFMWILGVSAFIGNIFVIIER--VREKKNFRVQSFLIFNLAISDCLMGVYMLIIASADVHYKSVYVFHAESWKASGMCKLAGILANLSSEVSVLILTIISLDRFLCITFPFSQKHLGRKSVRITAAVIWSACLIYSVIPALPIPYFGERFYGR------SGVCLALPLTNVKPPGWQ-YAMSAIFINFIAMVTIIGCYIVIYVVAKRSGSTVRNNKQMASEFKLAVRTAIIVATDICCWLPIVILAFVSLSNSAIIPPTVYAWIAVFVLPINSAINPYLYTISTVDV 957
BLAST of G-protein coupled receptor GRL101 vs. TrEMBL
Match: A0A1S3HKE3 (G-protein coupled receptor GRL101 OS=Lingula unguis OX=7574 GN=LOC106155271 PE=4 SV=1) HSP 1 Score: 347.436 bits (890), Expect = 1.316e-93 Identity = 232/695 (33.38%), Postives = 348/695 (50.07%), Query Frame = 1
Query: 3322 DAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICN---GFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISK-----------EDCYKNPA-EIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDC-SEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICC-SLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLV--WFVWVI-------SIALFQHFY 5328
D I I +CD DCL E DE +C +F F + DN IS ++ CD DC +G+DE C + C ++C+N QCI C+ +DCID SDE + C+ GF+C +S CI + D DCPG S EDEI E Y A +I+ C+S CF + C++ + + + C EHLQNC ++QCP +F+C + YCI L VCDG +DCP GEDE + C CP FRC CL+ +D+CNG+ DC +DE+ C+I +CP C C GY C + + + +L R+ + L L++ FS ++ L LNIS NS+ + F L NL L +S N I +L F L+ L+ L + NP+ IE AF L + ++L + + I TF GM + + S+N+S N + ++ +F G+ L+ L I+ N+ + + + + + + ++ + ICC + C K D S+C DLL N +QV VW +GI L GN+ V+I+ I V K +T + + NLA +++ MGIY+I I S D +R ++ S W SS C AG + +++Q +VT + LI DR I I F + S + L+ W W+I I F++FY
Sbjct: 686 DGVCISIVHLCDGEPDCLAGE-DEANCAC--------DFGSF---SCADNSCISTSKVCDHRPDCVDGSDESFCIHPPCRS-DSFRCKNGQCIKQESVCDLTADCIDGSDESPENTCSCEKGFQCYTSECIRAQAADDRFPDCPGISQEDEIQDSVCGISIGYEIIEQLYCQKANDIR--CTSGHGHCFPRHLACIHDKESYKALNVCRGFEHLQNCETYQCPSMFKC-PVSYCIPLRQVCDGQEDCPRGEDEADILCSNYTCPGRFRCKGEKQ----CLHQRDVCNGVVDCILSDDDEKLCEITECPNNCTCRGYLTDCPGAGFMTLPLLPNNIRSFAF-GNNQLTLTNTTFSGYLMLIKLNISSNSLKLIPVYAFQHLNNLIELDISDNGIELLSDRTFAGLRNLKYLHLKRNPLAKIEVGAFSELNQVQLLDLSFLTIRTIYSGTFKGM-DAITSLNISFNKLSAVQDGAFKGLPKLNLLDISGNEG-VVVDTNAFESFSAQLLKSDNFGICCLARNKIDNCLPKVDEFSSCYDLLKNSFLQVAVWVVGITALFGNMGVLIYSIHVMYKGSLTTTGLYVTNLATANLLMGIYMITIGSMDATFRGRFAAFSAVWTSSATCQFAGCLSLLASQMAVTVLALIATDRLIAIAFSEKVPNMSPKVAVALLLGSWLFWLILGVIPTTGIDFFKYFY 1357 HSP 2 Score: 98.2117 bits (243), Expect = 1.958e-16 Identity = 69/265 (26.04%), Postives = 110/265 (41.51%), Query Frame = 2
Query: 2339 VSVNFTPV------KMNCEDGWWKYDKSCYKFEKRQLDISWNDSLEICKTYNSKLVQISNRNEMEFLRNI---SLSRWLSEIYSN--TSVVIFIGLRNHITMPKDYDYVEDLYRLTWTKDEPLTYHEWFQRSPSKTYAF----LSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPL---------YWSNWVKIPCESKISSASFICQKPQYIKNSKYYFTKELEKINDMDGAR----------CSENWFFIEKRCLMLIKINDF 3031
V VN+T V +++C +G+ ++ +CYKF +SW + C N LV I +R EM FL I R+ + + IFIGL++ E + WT P Y EW+ + Y + + +SLK + +LQP ++ C+ MM PL W+NW+K+PC S +C+KP + + + + D C + W +IE RCL L ++ F
Sbjct: 276 VFVNYTAVPRQIVERVHCTNGYVLFEGNCYKFSTFTRGLSWEQAESKCGLENGHLVSIQSRGEMRFLNRIINNHQKRYFGTMCDSYYQRGGIFIGLKSQ----------EKTFLFNWTDGSPYVYSEWYGPT---IYGYDQYGVPDSLKAVFKEDVLQPVAQETLQ-CSVMMAFSPLSNGSPHGKSRWANWIKVPCHRPFCCMSSVCKKPATSATAHFRDENVSADVFNADAKVKVLSPAHELYCPDKWVYIEGRCLFLYTLSTF 526
BLAST of G-protein coupled receptor GRL101 vs. TrEMBL
Match: A0A1S3JM70 (uncharacterized protein LOC106174476 OS=Lingula unguis OX=7574 GN=LOC106174476 PE=4 SV=1) HSP 1 Score: 342.813 bits (878), Expect = 3.223e-91 Identity = 257/839 (30.63%), Postives = 425/839 (50.66%), Query Frame = 1
Query: 3352 CDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQF---ISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDC-DICN------GFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTS------NKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGL--FQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEG-EDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTF---LGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSG----KPLKHLTVKNVYTNVYKICCSLASY-GTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIK-------YLVFLVWFVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXX-XXXXXXXXVTGYVLIFFHSC----------RTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSNVN 5733
C+ DCL E DEKDC VK +P + + C D+ I W ++CDF+EDC +G+DEK C+ C E +C QCI+ +K C+ + DC+D SDE C DI ++C S I DD+VDC G + EDE+ +D + + C S CFQ+ + CL+ ++ D C HL+NC +F CP ++CS +CI LH +CDGTKDCP G+DE + CK +C F+C + + CL + +C+G+ C++ +DE++C + + CKC G + C + + + +++ L++ F Q+ LG L++++N+I + G FD L NL L L N + ++ S F L L+ L + NPI ++ A ++T++ L + + +++ F G+ L +N+SNN+++++ ++SF G+N L L + + +ND++ L + T+ + T+ +K CC Y +C ++D S CEDL+AN +++ +W +GI ++GN +VIIWR R K S LI +L +SD FMG+YL+IIA AD YYR++Y S YW++S C AG + VS++ SV + +IT +RFI ++F + +K ++ + VI + Q+F ++ +G+CL Y NF+ + +V +V F + TR+ AG+ + K +++ +I CW+PIII +S ++ ++ W+ +MP+NS+INP LYTFS +
Sbjct: 1210 CNGIRDCLDGE-DEKDCDACVKPEPEEGPKAYFYCETYDDSSPMCIPWAKFCDFVEDCHDGSDEKWCTQHLCLP-GERECDKGQCIEETKWCDGIVDCLDESDELTCNDIITDVNSNTSYQCFSGNIIPINQTFDDVVDCVGFTSEDEM---KDGEYTISGVNVTCDEGSFSCLGRYGCFQAESLCLFDLDSFGHLDHCRTGPHLKNCETFICPPQTHYKCSN-SFCIPLHRLCDGTKDCPGGDDEGD-YCKRFECKGMFKCPTERN----CLPWEKVCDGVIHCNKTHDDERFCGMEEACRLCKCSGSHVDCSKGERLTSIPWATRELRALTFRGNNISLTNETFVQYRRLGALDLANNNIKTIPKGTFDPLVNLIELDLRNNLLTVITSKVFSKLFALRKLILFGNPIMFVQEGAIALCTNLTYMNLSHLDIRNVSENAFKFDAGIKPRLRYLNLSNNNLKTLRKESFSGLNVLEELYLYLEENDLSQVKIDVFSTLPNTTI--LKTDDFKFCCIAKGYVESCEPRTDPFSTCEDLMANIGLRIAIWVLGILSILGNALVIIWRFRSERGKVVS---FLIQSLGMSDFFMGLYLLIIAGADVYYRNRYAIESDYWRNSGLCKFAGVMSMVSSEMSVFMLVIITYERFIVVLFPFKIERRMNMKTARIVVGVGFVVIVILSVIPVLGMQYF---GTEDYIKNGVCLLY-----------NFTAGKVKGWEYAAAIYLVANFVAFLFIAIAYIIIYWTVRSTRKQAGKAFE-EEAKLVRRITFVIATDFCCWMPIIITGVVSLLNDTPLDPTVTAWMVVFVMPLNSAINPILYTFSTIG 2017 HSP 2 Score: 63.929 bits (154), Expect = 5.801e-6 Identity = 52/210 (24.76%), Postives = 85/210 (40.48%), Query Frame = 2
Query: 2369 NCEDGWWKYDKSCYKF---EKRQLD------ISWNDSLEICKTYNSKLVQISNRNEMEFLRNISLSRWLSEIYSNTSVVIFIGLRNHITMPKDYDYVEDLYRLTWTKDEPLTYHEWFQRSPS--------------------KTYAFLSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPLYWSNWVKIPCESKISSASFICQKPQYIKN 2911
+C W ++ CYK + L +WN + E C+ + LV I + +E++FL + ++W +I +IFIG+ N + K Y TW + + +W+ + K + S + +++ LQP CTAM+ +NW+KIPCE ASFICQK +N
Sbjct: 792 SCAPNWQLWNDKCYKVFPNDNSTLTWIPGNRTTWNAANEQCELIGATLVSIRSSDELDFLMYMMATKWKDDIIHADKYIIFIGI-NDNKINKKY---------TWLDQSVMGFADWYNYQENRYLLEGRLLLEENQPICDGKKNTVATAISDRALVDMDKLQP-FSHEATRCTAMVFTDTHTSTNWIKIPCEFPFCKASFICQKDTNDRN 990
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Cavefish
Match: rxfp1 (relaxin family peptide receptor 1 [Source:ZFIN;Acc:ZDB-GENE-110125-1]) HSP 1 Score: 140.584 bits (353), Expect = 2.113e-33 Identity = 167/631 (26.47%), Postives = 287/631 (45.48%), Query Frame = 1
Query: 3937 QCP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNK----NDIAMSG-KPLKHLTV-----------------KNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXX-XXXXXXXXXXXVTGYVLIFFHSCR--TRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G DC G DEE+C + W H F D G+ C + P +C+C ++ C + ++ ++ +++ + +L++ +F ++ L L + N I + F GL+NL L LS N I L FQ L +L+ L + +N I + S F L S+ + L + L ++D L M L ++M N I ++ +F + L+ LV+ N+ +D A S + L L V VY ++ C +C +D S+ EDLLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I + D +R +Y ++ W SV C V G A +S + SV + +TL+++ICI+ FR + ++ ++W F+ ++ +F++FY ++G+C S + E + Q +SI++ L L+ +IV Y +F++ R T+ T + + AK+ ++++ LCWIPI IL LS + + +W+ ++PINS++NP LYT +
Sbjct: 29 HCPLGYFPCGNLSTCLPQLLHCNGVDDC--GNQADEENCG------DNNGWP--HLF-------DKYFGVPSNDPRTKSNTCLLGPVPAECQCRDLELDCDGAHFKDVPAVSINVTMMSLQRNRLRKLNADMFIKYQSLQKLYLQHNRIKVVEPHAFRGLYNLTRLYLSHNRISSLMPGVFQDLHKLEWLILENNKIQHVSSLTFSGLRSLVLLVLLNNALTRLDDVC-LEMPR-LNWLDMEGNQIETVENMTFWSCSMLTVLVLQRNRISHIHDQAFSVLRKLGELYVLSWFDLLLHFCQNNHQQSAVYFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLCCADSLMGVYLFVIGAYDLKFRGEYNRHAQAWMDSVPCQVVGSLAMLSTEVSVLLLTYLTLEKYICIVYPFRYLTPGRRRTVTILVVIWILGFIIAFLPLVCKEVFRNFY-------GTNGVCFPLHSEQPETAGAQIYSIVIFLGLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILS-LMDVEIPGTISSWVVIFILPINSALNPILYTLT 630
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Cavefish
Match: rxfp2l (relaxin family peptide receptor 2, like [Source:ZFIN;Acc:ZDB-GENE-060531-8]) HSP 1 Score: 127.487 bits (319), Expect = 1.455e-29 Identity = 154/622 (24.76%), Postives = 269/622 (43.25%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNF------------RCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPT-KCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIA-MSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDM--FMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIAL----------FQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G +DCP+G DE E+C P+ F C C K CNG ++ Q P C + + + Q L +GIF+ L ++ + +N I L F+GL +L + +L+ N + LP + + ++ N I+++ S +F N S+T + L ++ I D TF M LI ++S N I + F + NL ++ + +I +S + + L+ + Y ++ C + +C +D S+ E+LLAN ++VFVW + GN+ VI R +A + + T +AI + MG+YL I + D Y +Y ++ W S++C + G A +S + SV + +TL++++CI+F + + L L +W + L F +FY +G+C S + E Q +S + L L LIV Y +F+ +T ++A ++ ++ AK+ ++ +CWIPI +L LS + + + W+ ++PINS++NP LYT +
Sbjct: 7 CPLGQFPCGNLSICLPQLKHCNGQQDCPNGADE--ENCDNSGWPHLFDAFMKTRVQEPEECLDVYPEVCFCAGWKVNCNGK------------NLQQVPAVSCNVTAELNSNGLVSLPADLFIRYRRLERFLSQNRVSSLKAGIFTDLSSLEWI-LDENRITSLRQKSFEGLKSLFFSLLN-NSVEQLPKTSLCYEMPRLNWELEGNRISSLWSSSFKNCTSLT-VALRRNRISTIEDGTFSNM-HRLID-DLSVNQIMDLPPSLFQSLQNLKQR--SMEEVEIPNISIRMFRTLSNLSHYFKRFEYCGYSPNVRSCKPNTDGISSFENLLANIILRVFVWVVAFIICFGNVFVICLRSCIASENQHHT-------MAIKSLCCLMGVYLFFIGAFDIKYCGEYNRHAQIWMESLSCQLIGSLAMLSTEVSVMMLTYMTLEKYLCIVFPFQHYRPGRKRTLCSLT-SIWALGFILALIPFCDKQTFGNFYG-------RNGVCFPLHSDQAEKPGAQCYSTGIFLLNLFAFVLIVFSYTSMFYSVQKTAKSARQMAFDHEVAIAKRFFFIVFTDAMCWIPIFLLKILSLLRVQI--ATIILWVVIFILPINSALNPILYTIT 590
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Cavefish
Match: rxfp1 (relaxin family peptide receptor 1 [Source:ZFIN;Acc:ZDB-GENE-110125-1]) HSP 1 Score: 125.561 bits (314), Expect = 1.206e-28 Identity = 165/624 (26.44%), Postives = 290/624 (46.47%), Query Frame = 1
Query: 4111 CLNHKDLCNGISDCSEGEDEQYCD------IFQ----------------CPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYL-GYLNISDNSILELYNGLFDGLFNL-RYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITH--------------IELPSM-------KLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDI----------------AMSG-----------KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVW-------FVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRT--RRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLT---WLTAVLMPINSSINPYLYTFS 5724
CL CNG+ DC DE+ CD +F CP +C+C ++ C + ++ ++ T+++ + +L++ +F ++ L YL + ++E + F GL+NL RYL S N I L FQ L +L+ + + +N I + S F L S+ +E+P + ++ I+D F + + + ++S+N I++I + FV NL L+ N++ N I ++ G +PLK+LT Y ++ C +C +D S+ EDLLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I + D +R +Y ++ W SV C V G A +S + SV + +TL+++ICI+ FR + ++ ++W F+ ++ +F++FY ++G+C S + E + Q +SI++ L L+ +IV Y +F++ RT + T + + AK+ ++++ LCWIPI IL LS LM+ + T W+ ++PINS++NP LYT +
Sbjct: 37 CLPQLLHCNGVDDCGNQADEENCDNNGWPHLFDKYFGVPSNDPRTKSNTCPAECQCRDLELDCDGAHFKDVPAVSINV-TMSLQRNRLRKLNADMFIKYQSLQKYLQHNRIKVVEPHA--FRGLYNLTRYL--SHNRISSLMPGVFQDLHKLEWI-LENNKIQHVSSLTFSGLRSLVLVLLNNALTRLDDVCLEMPRLNWVLQRNRISHIHDQAFSVLRK--LGEDLSSNRIQTIPHNLFV---NLGDLLQNISYNPIQELQMDHFDKLHKLKSSIEGMEIGNIQRRMFEPLKNLTH---YFKKFQYCGYAPHVRSCKPNTDGISSFEDLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLC-ADSLMGVYLFVIGAYDLKFRGEYNRHAQAWMDSVPCQVVGSLAMLSTEVSVLLLTYLTLEKYICIVYPFRYLTPGRRRTVTILVVIWILGFIIAFLPLVCKEVFRNFY-------GTNGVCFPLHSEQPETAGAQIYSIVIFLLNLVAFLIIVLSYGSMFYNIQRTGTQTTKYSNHIKKELTIAKRFFSIVITDSLCWIPIFILKILS-----LMDVEIPTISSWVVIFILPINSALNPILYTLT 631
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Cavefish
Match: rxfp2a (relaxin family peptide receptor 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1225]) HSP 1 Score: 115.546 bits (288), Expect = 1.349e-25 Identity = 163/658 (24.77%), Postives = 279/658 (42.40%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWS--FNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIR--------------------------------ILPSYAFQFLK-QLQILD-------------ITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSG-KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIK---------YLVFLVWFVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTR-RTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLT---WLTAVLMPINSSINPYLYTFS 5724
CP G F C M C+ CDG +DC +G DE E C W+ F+ + + + +DL +D + + CD + T+ C G + LS N+ T ++ EL +FS++ L + +NSI + + F GLF LR +L N +R +P ++ F + ++++L+ + DN I T+ F +LIS+ +L + ++ F + L N+S+N + I F + L +++ + DI P+++L+ Y ++ C C +D S+ EDLLAN ++V VW M GN+ VI R + + I ++ +D MG+YL + D +R +Y N++ W SV C G+ A +S++ SV + +TL++F+ I+F K++ L FL+ V ++S LF ++Y +G+C S + E + +S + L LL +IV Y +F+ +T T R + D A + ++ LCWIPI ++ LS L+E + T W+ ++PINS++NP LYT +
Sbjct: 11 CPLGQFPCGNMSVCLPQMLQCDGQRDCKNGADE--EHCDN-------SGWADIFDRTIIKKSDPEDL----TDACLQQYPESCDCTK--TEVLCVGVHLRAVPLLSSNV--------TWSLKSNDLRELPDHVFSKYADLQRF-LQNNSIHTVSSHAFSGLFKLRK-VLDHNPLRNISHDTFFGLKSLTFSMVNTSLVQLSKLSICHYMPLLSWDFAENRIEVLNYSALMTCDGLTVSLHDNKIETLPENTFQSLISLAE-DLSCNMIADLPSNIFRSLKSLLK--NISHNTLLHIHAGHFDYLAQLESVLEGIEIPDIQTKMFLPMRNLSH---YFKDFQYCSYAPHVRKCKPNTDGISSFEDLLANMVLRVSVWVMAFITCFGNLFVIGMRSCIRAENSLHAACIKVL---CADCLMGVYLFFLGVFDVKFRGEYNRNALLWMESVECRTIGFLAMLSSEVSVLLLAYLTLEKFLVIVFPFSYLRPGKLQTGLVLVSIWLLGFLIAAVPLLSDDLFGNYY-------GRNGVCFPLHSDRLEKPTAKGYSTGIFLLNLLAFLVIVFCYSSMFYSIYKTGVNTDLRSRLHKDVAVANRFFFIVFSDALCWIPIFLVKILS-----LLEVEIPTINSWVVIFVLPINSALNPILYTLT 622
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Cavefish
Match: lrp1ab (low density lipoprotein receptor-related protein 1Ab [Source:ZFIN;Acc:ZDB-GENE-030131-7126]) HSP 1 Score: 105.145 bits (261), Expect = 7.892e-22 Identity = 92/287 (32.06%), Postives = 133/287 (46.34%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKL-CSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD-ICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNC--ISFQC-PGL-FQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDI 4185
+CD DC E DE + ++ P N+F PC SW CD +DC + +DE C+Y C LT++ C N +CI+V+ RC+ +DC DNSDE C C+ FKC S CI + D DC +S E +C C + +C + + C+ C L ++C ++ C P + F C CI +VCDG DC D DED +C+ L C + + N S +CL + LC+G DC +G DE+ CD+
Sbjct: 892 LCDGDNDCGTDE-DESNTTCSARTCPPNQF----PCASGRCIPSSWT--CDLDDDCGDRSDEPASCAYPTCFPLTQFTCANGRCINVNWRCDNDNDCGDNSDEAGCSHSCSSVQFKCNSGRCIPEYWTCDGDNDCGDNSDE----KSANCTNQATRPPGGCHTDEFQC-RMDGLCIPMRWRCDGDTDCMDLSDEKHCEGVTHMCDPAVKFTCRDSARCISKAWVCDGDSDCEDNSDED--NCEALMCKLSHHVCASNSS--ICLPAEKLCDGKDDCPDGSDEKLCDL 1162 HSP 2 Score: 90.1225 bits (222), Expect = 2.457e-17 Identity = 81/246 (32.93%), Postives = 113/246 (45.93%), Query Frame = 1
Query: 3478 ISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI--CNGF-----------KC-LSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE 4170
I + +CD +DC + +DE C+ + CA E+QC++ CI S RCN+V DC D SDE +C C+ F KC ++LC A ++ D DC S E +C PA+ K C C + C+ S + C +C F P F+C + CI +VCDGT DC DG DED CK+ C P F C +C+ + C+G DC +G DE
Sbjct: 2532 IKHSSWCDGTDDCGDHSDELPCNMTMCA-AAEFQCKDGSCISNSSRCNQVVDCEDASDELNCSPTDCSSFYLLGVKSMTFQKCEFTTLCYAPSWQCDGSNDCGDFSDE------RNC---PAKRKQKCPVNFFAC--PSGRCIPMSWTCDKENDCENGTDETHCDKFCSPTQFECGNHR-CITSSWVCDGTNDCGDGTDEDSR-CKSKTCSPEAFHC----PDSYICIPQRWKCDGDKDCPDGADE 2759 HSP 3 Score: 74.7146 bits (182), Expect = 1.328e-12 Identity = 80/284 (28.17%), Postives = 126/284 (44.37%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDE-KLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDC--DIC--NGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCP--YNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYC 4179
+CD DC+ ++ +C ++EF+ ++ + I CD +DC + +DE K R E ++ C+N +C+ +C+ +DC DNSDE+DC C + F C + CIA ++ D DC S E+ K D + + C +C ++ C+ S+ S CP E FQC+ C L + CDG DC D DE+ E C+ CP NFRC + +CL + C+G++ C + DE C
Sbjct: 3425 MCDRDNDCVDGSDEPANCTQMTCG--VDEFR-----CKDSGRCIPARWKCDGEDDCGDSSDEPKEECDERTCEPYQFPCKNNRCVPGRWKCDYDNDCGDNSDEEDCVPRQCSESEFACTNGRCIAGRWKCDGDHDCSDGSDENGCDLKCDVDQFQCK-NGHCIPLRWRC-DADADCMDGSDEERSERLCPNDE-------------FQCNNT-LCKPLAWKCDGEDDCGDNSDENPEECRKFVCPPTRNFRC----QNDRVCLWNGKRCDGVNHCGDNSDELNC 3681 HSP 4 Score: 70.8626 bits (172), Expect = 1.633e-11 Identity = 82/282 (29.08%), Postives = 125/282 (44.33%), Query Frame = 1
Query: 3385 NDEKDCIVKVKSK-PMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDE------KDCDICNGFKCLSS-LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC--PYNFRCWSFNH--SFLLCLNHKDLCNGISDCSEGEDEQYCDIFQC 4194
+DE++C K K K P+N F C +SW CD DC+NGTDE C + T+++C N +CI S C+ +DC D +DE K C F C S +CI ++ D DCP + E +K C N + + +C + C + + D P E+ C P F+C+ + + ++ CDG DC D+ +E+ K C P N RC H + C++ + LC+ DC +G DE C + +C
Sbjct: 2636 SDERNCPAKRKQKCPVN----FFACPSGRCIPMSWT--CDKENDCENGTDETHCD--KFCSPTQFECGNHRCITSSWVCDGTNDCGDGTDEDSRCKSKTCSP-EAFHCPDSYICIPQRWKCDGDKDCPDGADEG---AKAGCSTNTTCDDTEFQCQNKQCIPKHFVCDHDLDCRDGSDESPECEY-PTCG----PNDFRCANGQCLKQKNWECDGEFDC---HDQSDEAPKNTHCTEPEN-RCNDTAHLCTNGKCVSEQVLCDRNDDCGDGSDELNCFVNEC 2896 HSP 5 Score: 61.2326 bits (147), Expect = 1.527e-8 Identity = 72/254 (28.35%), Postives = 106/254 (41.73%), Query Frame = 1
Query: 3478 ISWNRYCDFIEDCKNGTDEK--LCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI----CNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESC---KTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKC 4212
I+++ CD CK+ +DEK CS C + +C N +CI S C+ DC D+SDE C++ F+C CI++ + +VDC +S DE+ CS T F LL + ++FQ +C C + CDG+ DC G+ DE +C + KCP NF F C+ C+ +DC G DE +CD F PT+ +C
Sbjct: 2491 INYSLTCDGRAHCKDKSDEKPSYCSNRGCKKGYR-RCLNGRCIKHSSWCDGTDDCGDHSDELPCNMTMCAAAEFQCKDGSCISNSSRCNQVVDCEDAS--DELN---------------CSPTDCSSFY-----------LLGVKS----------MTFQ-----KCEFTTLCYAPSWQCDGSNDC--GDFSDERNCPAKRKQKCPVNF----FACPSGRCIPMSWTCDKENDCENGTDETHCDKFCSPTQFEC 2694 HSP 6 Score: 59.6918 bits (143), Expect = 3.845e-8 Identity = 79/294 (26.87%), Postives = 113/294 (38.44%), Query Frame = 1
Query: 3460 RNDNQFISWNRYCDFIEDCKNGTDE-KLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI----CNGFKCL-SSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGL--FQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC-PYNFRC---------WS-------------------------FNHSFLLCLNHKDLCNGISDCSEGEDEQYCDI------FQC 4194
+ND W + CD +DC + +DE + C +C ++QC C + + C+ DC D SDE +CDI + FKC S CI +F + +C EDE +DC + + +C C ++ + D NC C G+ F+C CI + CDG DC D DE +E C C PY F C W F + C+ + C+G DCS+G DE CD+ FQC
Sbjct: 3295 KNDKCIPFWWK-CDTEDDCGDRSDEPEDCPAFKC-RPGQFQCVTGICTNPAYICDGDDDCQDLSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQNNC--GQGEDE----KDCPEVTCAPNQFQCVITKRCIPRVWMCDRDNDCVDGSD------EPANCTQMTC-GVDEFRCKDSGRCIPARWKCDGEDDCGDSSDEPKEECDERTCEPYQFPCKNNRCVPGRWKCDYDNDCGDNSDEEDCVPRQCSESEFACTNGRCIAGRWKCDGDHDCSDGSDENGCDLKCDVDQFQC 3573 HSP 7 Score: 55.8398 bits (133), Expect = 6.290e-7 Identity = 63/229 (27.51%), Postives = 99/229 (43.23%), Query Frame = 1
Query: 3568 TEWQCQNKQCIDVSKRCNKVSDCIDNSDE-KDCDI----CNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKN---PAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYN-FRCWSFNHSFLLCLNHKDLCNGISDCSEGE-------DEQYCDIFQCPTK 4203
+++ C+N +CI +C+ DC D SDE +DC F+C++ +C + D DC ++ + +C + P++ K CS S +C C +N C E ++C C P FQC K CI ++CD DC DG DE +C + C + FRC C+ + C+G DC + DE+ C+ +Q P K
Sbjct: 3290 SQFVCKNDKCIPFWWKCDTEDDCGDRSDEPEDCPAFKCRPGQFQCVTGICTNPAYICDGDDDC------QDLSDEANCDIHVCLPSQFK--CSHPS-RCIPGIFRCNGQNN-------CGQGEDEKDCPEVTCAPNQFQCVITKRCIPRVWMCDRDNDCVDGSDE-PANCTQMTCGVDEFRCKDSGR----CIPARWKCDGEDDCGDSSDEPKEECDERTCEPYQFPCK 3497 HSP 8 Score: 54.6842 bits (130), Expect = 1.316e-6 Identity = 31/82 (37.80%), Postives = 44/82 (53.66%), Query Frame = 1
Query: 3943 PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESC---KTLKCPYN-FRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQY 4176
P F C CI + CDG KDCPDG DE + C + +CP N F C + +C++ +C+ ISDC++G DE +
Sbjct: 14 PKQFVCKDQVTCISKGWRCDGEKDCPDGSDESPDICHQSQASQCPPNEFECRGTD----VCIHLSKVCDSISDCTDGRDEGH 91
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Sea Lamprey
Match: lrp2a (low density lipoprotein receptor-related protein 2a [Source:ZFIN;Acc:ZDB-GENE-050119-2]) HSP 1 Score: 104.76 bits (260), Expect = 1.856e-22 Identity = 85/290 (29.31%), Postives = 136/290 (46.90%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNG----FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACP--LLEHLQNCISFQC--PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLK----CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCD 4182
VC+ DC +DE+ C ++ P+ +++ C+ + + W CD +DC +G+DE+ C+ C+E +E++C N++CI CN +DC DNSDE+DC++ F+C S CI + D DC S E ++ Y N + C +S +C N+ C+ S + C E L +C+ C PG F+C + CI H +C+ DC DG DE + CK F+C S C+ +CN ++C + DE C+
Sbjct: 3663 VCNGMDDCR-DNSDEQGC-AEITCDPLGDYR----CDNHRCIPLRWR--CDRYDDCGDGSDERNCAPRPCSE-SEFRCTNQRCIPGRWVCNHENDCGDNSDERDCELQTCLPGYFQCGSGHCIPERLKCDGSADCQDFSDESLCPTR---YPN----GTWCPPSSFEC--DNHVCIPPSWKCDGDNDCGDGSDEELHHCLDTPCNPPGRFRCDNNR-CIYSHEICNAVDDCGDGSDETPDKCKEPTFAPCTEEEFKCASGR-----CIPMAAVCNNYNECRDNTDETGCN 3928 HSP 2 Score: 91.6633 bits (226), Expect = 1.752e-18 Identity = 74/259 (28.57%), Postives = 116/259 (44.79%), Query Frame = 1
Query: 3454 CNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDC--DIC--NGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKS-LCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC--PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCK 4209
C N +W C+ ++DC++ +DE+ C+ C L +++C N +CI + RC++ DC D SDE++C C + F+C + CI + + DC G + ++ + C + S C KC S + + +L CP N ++ P F+C CI + CDG DC DG DE+ C C P FRC + C+ ++CN + DC +G DE P KCK
Sbjct: 3651 CRTNYRCIPTW-AVCNGMDDCRDNSDEQGCAEITCDPLGDYRCDNHRCIPLRWRCDRYDDCGDGSDERNCAPRPCSESEFRCTNQRCIPGRWVCNHENDC-GDNSDERDCELQTCLPGYFQCGSGHCIPERLKCDGSADCQDFSDESL-----CP--TRYPNG-TWCPPSSFECDN-HVCIPPSWKCDGDNDCGDGSDEELHHCLDTPCNPPGRFRCDNNR-----CIYSHEICNAVDDCGDGSDET-------PDKCK 3886 HSP 3 Score: 84.7297 bits (208), Expect = 2.301e-16 Identity = 73/241 (30.29%), Postives = 106/241 (43.98%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI-CNG--FKCLS-SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACP--LLEHLQNCISFQC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQ-YCDIFQC 4194
CD DC +G+DE+ C S ++QC + +CID + C+ SDC+D +DE+ C C F C S CIA+ + D DC S DE+ C P LC + +C QS+ C+ C EH C C G +C C+ + CDG DC DG DE + +C + NH C++ + +C+G DC +G DE +C+ C
Sbjct: 1090 CDTDNDCGDGSDERGCDMSGTCHPMQFQCPDHRCIDNAYVCDGDSDCVDGADERGCVFNCTSYQFSCSSGDQCIATYYRCDGAFDCRDRS--DEV----GC---PTRAPGLCHNDEFQC-QSDGLCIPAGWECDGHPDCDDGSDEH-PGCPPRTCAAGYLRCDNGN-CVPESWRCDGDNDCRDGTDERDCPTPPYRCQSPSQWLCPNHR--ACVDLRKVCDGNFDCPDGGDESPHCNEHDC 1316 HSP 4 Score: 81.6481 bits (200), Expect = 2.165e-15 Identity = 72/253 (28.46%), Postives = 111/253 (43.87%), Query Frame = 1
Query: 3478 ISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDN--SDEKDCD--ICNG--FKCLSS-LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCIS---FQCPGL-FQCSKMKYCIELHYVCDGTKDCPDGEDED-EESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE-QYCDIFQC 4194
+ + CD DC + +DE+ C++ C E+ C N +CI S+ CN V++C DN SDE+ C C KCL++ +C+ + G + E + + + P + +N+ N C+ S S C + S CPG F C + CI ++CDG DC DG DED +C C P+ F C + C+ + +C+G +DCS+ DE Q C C
Sbjct: 2715 VRYGYRCDHTNDCGDSSDEEGCAFRTCDPAAEFACTNGRCIARSQVCNGVNNCRDNATSDEQRCPERTCQPGLLKCLTTNICVLRHY-------LSGGAAE----WRRNDLRRPPPLPLHTCFIANEFRCGNGQCIPLSWVCDSEQDCTDNADEPSSCSDPGRTCPGTQFTCDSGR-CIPGSWICDGDNDCGDGSDEDGRHACGARTCGPHEFTCADASPPRRRCIQRRFVCDGDADCSDALDEHQNCTRRSC 2955 HSP 5 Score: 77.411 bits (189), Expect = 3.985e-14 Identity = 80/265 (30.19%), Postives = 110/265 (41.51%), Query Frame = 1
Query: 3319 IDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRN-DNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDC-----DICNG--FKCLSS-LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCIS----FQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYN 4074
I YWI CD DC +DE+ C + PM +FQ P +R DN ++ CD DC +G DE+ C ++ C QCI RC+ DC D SDE C +C+ F+C S LCI + +E D DC S E +P C++ +C N C+ S + C ++C + Q P + C + C++L VCDG DCPDG DE C C N
Sbjct: 1084 IPRYWI-----CDTDNDCG-DGSDERGCDMSGTCHPM-QFQ--CPDHRCIDNAYV-----CDGDSDCVDGADERGCVFN-CTSYQFSCSSGDQCIATYYRCDGAFDCRDRSDEVGCPTRAPGLCHNDEFQCQSDGLCIPAGWECDGHPDCDDGSDE-----------HPGCPPRTCAAGYLRC--DNGNCVPESWRCDGDNDCRDGTDERDCPTPPYRCQSPSQWLCPNHRACVDLRKVCDGNFDCPDGGDESPH-CNEHDCSVN 1319 HSP 6 Score: 73.9442 bits (180), Expect = 3.975e-13 Identity = 76/273 (27.84%), Postives = 107/273 (39.19%), Query Frame = 1
Query: 3430 NEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDE-KLCS-YSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNG-------FKCLSS-----LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLK---CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQC 4194
NEF+ C +SW CD +DC + DE CS R T++ C + +CI S C+ +DC D SDE C F C + CI F D DC D + ++C + C++ C N C++ R+ C + C C F C + CI + CDG DC DG DED C P FRC + C+ +CN +DC++G DE+ C I +C
Sbjct: 2829 NEFR----CGNGQCIPLSW--VCDSEQDCTDNADEPSSCSDPGRTCPGTQFTCDSGRCIPGSWICDGDNDCGDGSDEDGRHACGARTCGPHEFTCADASPPRRRCIQRRFVCDGDADC-----SDALDEHQNCTRRS------CTAGEFAC--DNGLCVHSRYRCDRRNDCGDGSDERGCTYPTCRQNQFTCENGR-CIPRIFACDGDNDCGDGSDEDTHLCHQPSPTCAPGFFRCGAGE-----CVAIHLVCNQANDCADGSDEKGCGINEC 3076 HSP 7 Score: 72.4034 bits (176), Expect = 1.412e-12 Identity = 71/270 (26.30%), Postives = 103/270 (38.15%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSR--CAELTEWQCQNKQCIDVSKR---------------------------CNKVSDCIDNSDEKDCDICNG-----FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTC--LYHSNNLLSRDACPLLEHLQNCISFQCPGL-----FQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNF-RCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYC 4179
CD +DC +G+DE+ C C+ + C N++CI + R C+ +DC D SDE+ CD+ F+C CI + + D DC + E C N + CSS ++C + C + + CP + PGL FQC CI + CDG DC DG DE C C + RC + N C+ C+G +DC +G DE+ C
Sbjct: 1022 CDGYDDCHDGSDERGCGTGNVTCSPYA-FTCTNRRCIPIDWRPTRLATTCRADQFTCASGNRCIPRYWICDTDNDCGDGSDERGCDMSGTCHPMQFQCPDHRCIDNAYVCDGDSDCVDGADE------RGCVFNCTSYQFSCSS-GDQCIATYYRCDGAFDCRDRSDEVGCPT----------RAPGLCHNDEFQCQSDGLCIPAGWECDGHPDCDDGSDE-HPGCPPRTCAAGYLRCDNGN-----CVPESWRCDGDNDCRDGTDERDC 1267 HSP 8 Score: 72.0182 bits (175), Expect = 1.847e-12 Identity = 85/340 (25.00%), Postives = 127/340 (37.35%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDE-KLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD--ICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISK-----------------------EDCYKNPAE---IKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISF-----QC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDE---------ESCKTLKCPYNFRCWS-------FNHSFLLCLNHKDLCNGISDCSE-GEDEQYCDIFQCPTKCKC 4212
VCD DC ++DE+ C P C R+DN+ I+ CD DC + +DE + C S C+ + + C + C+ + RC+ DC D SDE+ C C C + C + D +V+C S E ++ +DC E + C C ++ C+ S D C + C+S +C P + C K CI VCDG +DCP G DE E+C L C Y RC + + + N+ C DC G +Q+C+ C+C
Sbjct: 19 VCDSINDC-SDDSDERGC------SPATCTTLQFRC-RSDNRCIARAFVCDGEADCADRSDEHQNCPGSTCSS-SHFTCASGHCVQSAFRCDHFQDCDDRSDEQSCQYPTCAEMTCANGACFNTAQRCDGVVNCRDLSDEANCTARCPVGEFQCANERCVPQSYRCDHIDDCTDGSDENGCVYGTCQGNQFTC--ASGRCILQSWVCDGDDDCFDGSDERGCVSAASEERECYPEEWPCPGSKACIPFRKVCDGRRDCPHGTDESNSTAGLACSIENCGALSCEY--RCHASPAGGACYCPPSFVVSNNSITCMDFDDCKMWGVCDQFCENRPAGHMCQC 345 HSP 9 Score: 69.707 bits (169), Expect = 8.159e-12 Identity = 66/260 (25.38%), Postives = 90/260 (34.62%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD--IC--NGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDED---------------EESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGED--EQYCDIFQCPTKCKC 4212
CD DC + DE R E+ C N C+ RC++ +DC D SDE+ C C N F C + CI +F D DC S ED ++ C Q + TC PG F+C + C+ +H VC+ DC DG DE +C + CW+ F L N + C I +C+E Q C+ CKC
Sbjct: 2934 CDGDADCSDALDEHQNCTRRSCTAGEFACDNGLCVHSRYRCDRRNDCGDGSDERGCTYPTCRQNQFTCENGRCIPRIFACDGDNDCGDGSDED----------------------THLCHQPSPTC--------------------------APGFFRCGAGE-CVAIHLVCNQANDCADGSDEKGCGINECLYASINQCAHNCTNTLTSFYCSCWA---GFRLMANGR-ACEDIDECNETPQVCSQLCENTAGSYFCKC 3140 HSP 10 Score: 60.8474 bits (146), Expect = 4.273e-9 Identity = 74/297 (24.92%), Postives = 114/297 (38.38%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDIC-------NGFKCLSSLCIASMFEMDDIVDCPGSSMEDE-IISKEDC-------------------------YKNPAEIKSLCSSTSNKC-------FQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCP--GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYN-FRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDI-------FQC------PTKCKCFG 4218
CD DC++ +DE R + +QC++ C + CN ++C D SDE+D +C N ++C++ CI + + D DC S ED + C E C S++C ++N C+ D C Q C C G ++C + CI L + CD DC DG DE +C C + FRC + C+ + +CN +DC + DE+ C++ FQC P + KC G
Sbjct: 3497 CDGQRDCRDASDEPATCPHRYCPIGRFQCRDGNCTSSNLLCNGNNNCPDGSDEEDT-LCFNHRCNSNQWQCMNKRCIPAAWLCDGGPDCQDGSDEDSATCAARTCAPGQFRCNNGRCIPRSWVCDVDDDCGDGSDEPHDTCMGPSHRCDSHTEFACRTNYRCIPTWAVCNGMDDCRDNSDEQGCAEITCDPLGDYRCDNHR-CIPLRWRCDRYDDCGDG--SDERNCAPRPCSESEFRCTNQR-----CIPGRWVCNHENDCGDNSDERDCELQTCLPGYFQCGSGHCIPERLKCDG 3784 HSP 11 Score: 60.4622 bits (145), Expect = 5.097e-9 Identity = 64/224 (28.57%), Postives = 90/224 (40.18%), Query Frame = 1
Query: 3571 EWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD--ICNG--FKCLS-SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNC------ISFQCPGLFQCSKMK----YCIELHYVCDGTKDCPDGEDEDEESCKTLKCPY-NFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQC 4194
+++C + CI S C+ ++DC D+SDE+ C C F+C S + CIA F D DC S E + C S + S C QS C +H Q+C S Q P C++M C CDG +C D DE +C T +CP F+C + C+ C+ I DC++G DE C C
Sbjct: 5 QFRCGSGACIPRSWVCDSINDCSDDSDERGCSPATCTTLQFRCRSDNRCIARAFVCDGEADCADRSDEHQNCPGSTCS------SSHFTCASGHCVQSAFRC----------------DHFQDCDDRSDEQSCQYP---TCAEMTCANGACFNTAQRCDGVVNCRD--LSDEANC-TARCPVGEFQCANER-----CVPQSYRCDHIDDCTDGSDENGCVYGTC 195 HSP 12 Score: 54.299 bits (129), Expect = 3.839e-7 Identity = 47/192 (24.48%), Postives = 70/192 (36.46%), Query Frame = 1
Query: 3634 CIDNSDEKDCDICNG---FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPT 4200
C DN E C+ F C + C+ +F D DC S E C N TC ++ +R P+ + F C+ CI +++CD DC DG DE P F+C C+++ +C+G SDC +G DE+ C +F C +
Sbjct: 990 CADNPLEPPTPQCSSTEFFACANGRCVPRLFLCDGYDDCHDGSDE------------------------RGCGTGNVTCSPYAFTCTNRRCIPIDWRPTRLATTCRADQFTCASGNRCIPRYWICDTDNDCGDGSDERGCDMSGTCHPMQFQCPDHR-----CIDNAYVCDGDSDCVDGADERGC-VFNCTS 1151 HSP 13 Score: 51.2174 bits (121), Expect = 2.950e-6 Identity = 33/122 (27.05%), Postives = 60/122 (49.18%), Query Frame = 1
Query: 3352 CDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDE-----KLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLC 3702
CD DC ++E + P F+ C+ +N+ I + C+ ++DC +G+DE K +++ C E E++C + +CI ++ CN ++C DN+DE C+ C +C
Sbjct: 3826 CDGDNDCGDGSDEELHHCLDTPCNPPGRFR----CD--NNRCIYSHEICNAVDDCGDGSDETPDKCKEPTFAPCTE-EEFKCASGRCIPMAAVCNNYNECRDNTDETGCNQGQDRTCAERIC 3940
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Sea Lamprey
Match: tshr (thyroid stimulating hormone receptor [Source:ZFIN;Acc:ZDB-GENE-110524-4]) HSP 1 Score: 100.523 bits (249), Expect = 1.519e-21 Identity = 86/377 (22.81%), Postives = 178/377 (47.21%), Query Frame = 1
Query: 4276 TRTLTIVQTHSLELSSGIFSQFIYLGYLNISD-NSILELYNGLFDGLFNLRYLILSKNYIRILPSYA-FQFLKQLQILDITDNPI------TTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDI---------------AMSGKPLKHLTVKNVYTNVYKI-------CCSL----ASYG------------------------TCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIF 5232
T+ +V H L + FS+ L + I + N ++ ++ F L +LR+L ++ I P Q IL+I DNP+ ++ + +LI H E ++ I+++ G +L+ ++ +N+ + +I +D+F G+ ++ +++V++ + A + LK L ++++ CC+ A+ G C+ + D + CEDL+ ++ +++ VW + + ++GN +VI+ + ++ + K + L+ NLA +D+ MG+YL+ IAS D Y R +Y ++I W++ + C +AG+ S++ SV + +IT++R+ II+
Sbjct: 22 TKIDILVSRHLRSLEAMSFSRLSQLQDIEIRNANRLVYIHRDAFHELPSLRFLGIANTAIECFPHVGKIQSTVLSFILEILDNPMIDLIPANALQGLSSGSLIFKEHTE----EVGRISEFVMAGNLPDLLDLH-NNDRLWAIGEDAFSGIQE-NSFILDVSRTSVRSLPRHGLQHVWKLMANAAWHLKQLPPMGSFSSLCSANLTYPSHCCAFWHRKAAQGCHADIYMDQELQNESFTSSDFDLVQCAPEPDAFNPCEDLMGHQLLRLSVWFVSVLAVLGNSIVIL--VILSSRYKLAVPRFLMCNLAFADLCMGVYLLTIASFDVYTRGEYHNHAIDWQTGLGCRLAGFLTVFSSELSVFTLTVITVERWHTIIY 390
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Sea Lamprey
Match: lrp1ab (low density lipoprotein receptor-related protein 1Ab [Source:ZFIN;Acc:ZDB-GENE-030131-7126]) HSP 1 Score: 94.3597 bits (233), Expect = 2.926e-19 Identity = 91/288 (31.60%), Postives = 132/288 (45.83%), Query Frame = 1
Query: 3334 IGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKL-CSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD---ICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNC--ISFQC-PGL-FQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE 4170
I + +CD DC SE DE + ++ P N+F C +SW CD +DC + +DE C+Y C LT++ C N +CI+++ RC+ +DC D SDE C+ + FKC S CI S + D DC S E + +C K C +C +++ C+ C + C + C P L F C CI + CDG DC D + DEE C+T+ C P + C + +CL+ K LC+G SDC +G DE
Sbjct: 881 IPLRWLCDGDNDCGNSE-DESNTTCAARTCPPNQFS----CASGHCIPMSWK--CDLDDDCGDRSDEPPSCAYPTCFPLTQFACNNGRCINMNWRCDADNDCADASDEIGCNHSCSISQFKCNSGRCIPSHWTCDGDNDCGDFSDE----TNPNCTKLATRPPGGCHGDEFQC-KADGQCIPLRWRCDGDTDCIDTSDEKECDGVVRTCDPTLQFSCRDTGRCISKTWFCDGDNDCED--NSDEEGCETMLCKPPSHSC---ANDSSICLSPKKLCDGHSDCPDGSDE 1151 HSP 2 Score: 83.9593 bits (206), Expect = 4.499e-16 Identity = 82/260 (31.54%), Postives = 114/260 (43.85%), Query Frame = 1
Query: 3466 DNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI---CNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNT-CLYHSNNLLSRDACP--LLEHLQNCISFQCPG--LFQCSKMKYCIELHYVCDGTKDCPDGEDE---DEESCKTLKC-PYNFRCWSFNHSFLLCLNHKDLCNGISDCSE-GEDEQYCDIFQCPTKC 4206
+N+ + CD+ DC + +DE+ C+ C+E +E+ C N +CI RC+ DC D SDEKDC + F+C + CI + D +DC S ED N A I C FQ NNT C S D C E + C+ FQCP F+C + C CDG +C DG DE ++ S K C F+C C++ + +CN DC + G DE C Q +C
Sbjct: 3514 NNRCVPGRWQCDYDNDCGDNSDEEGCTPRPCSE-SEFSCANGRCIAGRWRCDGDHDCSDGSDEKDCSPRCDHDQFQCRNGHCIPQRWRCDADIDCIDGSDED----------NCAAIVRTCPHHE---FQCNNTLCKPLSWKCDGEDDCGDNSDETPEMCLKFQCPPGRQFRCRNNRVCFWAGRQCDGVDNCGDGSDEENCEKPSPKPRICNSSEFKCGGTERK---CISTQLVCNRFDDCGDGGSDEVACGGDQFVNEC 3756 HSP 3 Score: 70.4774 bits (171), Expect = 4.307e-12 Identity = 79/290 (27.24%), Postives = 114/290 (39.31%), Query Frame = 1
Query: 3370 CLISENDEKDCIVK-----------VKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEK--LCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCD--ICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIF 4188
CLI+E+ +C + V K + Q CN D I ++ CD + DCK+ +DEK C+ +C C N +C++ K CN V+DC DNSDE C+ C F+C CIA++ + +VDC + DEI C N T YH + Q G C+ C+ ++CDG DC G+ DE++CK FN C+ C+ DC +G DE CD+
Sbjct: 2470 CLITEDGRVNCKCRGERVLVDGTRCVARKNSCDLQTEFECNNGD--CIRYHLTCDGVADCKDKSDEKPFYCTSRQCKNGYV-NCLNGRCMETLKLCNGVNDCGDNSDEIACNNSTCGADEFRCGDGSCIANVTRCNQVVDC--DDISDEI----------------------GCGPMNCT-HYHR------------------LGVQASGYVSCNSTSLCVLPAWICDGANDC--GDYADEKNCKKKSGSPGCSPDYFNCPSGPCIPTAWKCDREPDCDDGADELNCDML 2711 HSP 4 Score: 55.4546 bits (132), Expect = 1.880e-7 Identity = 37/100 (37.00%), Postives = 47/100 (47.00%), Query Frame = 1
Query: 3943 PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNH----SFLLCLNHKDLCNGISDCSEGEDE--------QYCDIFQCPTKC 4206
P F C CI L + CDG KDCPDG DE E C+ P +C NH LC++ LC+G +DC +G DE + C + C KC
Sbjct: 7 PTQFVCKDGLTCISLGWRCDGEKDCPDGSDESPEICQK---PNEMKC-QVNHYHCLGTELCIHMSKLCDGNNDCPDGYDEGTHCREFAENCTVTLCRHKC 102 HSP 5 Score: 55.0694 bits (131), Expect = 2.459e-7 Identity = 72/276 (26.09%), Postives = 107/276 (38.77%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLC---SYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDI---CNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPA--EIKSLCSSTSNKCFQSNNTCLYHSN--------NLLSRD---------ACPLLEHLQNCISFQ-----CPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCW----------SFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTK 4203
CD DC + DEK C S S + C + CI + +C++ DC D +DE +CD+ + F+C + CI + D DC S E + N A + TS+ + CL + N+ +R A L H +SF P F+C+ + ++ + CDG DC D DE P N C SF + C+N LCN DC +G DE+ C+I +C +
Sbjct: 2650 CDGANDCGDYADEKNCKKKSGSPGCSPDYFNCPSGPCIPTAWKCDREPDCDDGADELNCDMLCAVDHFECDNGKCITKRWMCDGEDDCGDRSDEKDAHCTFRACANIAYSALHLTIVITSHWLHNHSRCCLLPTEQSIYMRILNVSTRKHVCVCVPMHARRLSVHSSLTVSFADYPMCGPNQFRCANGRCLLKSSWECDGDFDCHDRSDE---------APINPHCSANNSKCNDSSSFPCANGRCVNESMLCNNQDDCEDGSDERNCNINECADR 2916 HSP 6 Score: 54.299 bits (129), Expect = 4.069e-7 Identity = 54/175 (30.86%), Postives = 76/175 (43.43%), Query Frame = 1
Query: 3580 CQNKQCIDVSKRCNKVSDCIDNSDE-KDCD----ICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQC-PGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPY-NFRC 4083
C+N +CI +C+ DC D SDE DC F+C + +C F D DC G ++ C P++ K T+++C ++ N +D C E ++C C P FQCS K CI +VCD DC DG DE +C + C FRC
Sbjct: 3310 CKNDKCIPFWWKCDTEDDCGDRSDEPADCPDFKCRPGQFQCKNGICTNPAFICDGDNDC-GDGSDEANCDVHVCL--PSQFKC---RTTHRCIPG----IFRCN---GQDNCGDGEDEKDCPEVTCAPNQFQCSSTKRCIPRVWVCDRDNDCGDGSDE-PANCTQMTCGQEEFRC 3470 HSP 7 Score: 53.1434 bits (126), Expect = 8.096e-7 Identity = 76/288 (26.39%), Postives = 114/288 (39.58%), Query Frame = 1
Query: 3496 CDFIEDCKNGTDEKLCSYSRCAELTEWQCQN-KQCIDVSKRCNKVSDCIDNSDE-KDCD---------ICNG-------------------------------------FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYH---SNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYN-FRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE--QYCDIFQCP 4197
C+ ++C +G DEK C CA ++QC + K+CI C++ +DC D SDE +C C G F+C ++ C+ ++ D DC +S E E C P +S S + +C C S+ +D P +H Q FQC + +CI + CD DC DG DED + CP++ F+C N++ L+ K C+G DC + DE + C FQCP
Sbjct: 3402 CNGQDNCGDGEDEKDCPEVTCAP-NQFQCSSTKRCIPRVWVCDRDNDCGDGSDEPANCTQMTCGQEEFRCKGTGRCIPSRWKCDGDDDCGDTSDEPKEECEDRTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDE------EGCTPRPCS-ESEFSCANGRCIAGRWRCDGDHDCSDGSDEKDCSPRCDHDQ----FQC-------RNGHCIPQRWRCDADIDCIDGSDEDNCAAIVRTCPHHEFQC---NNTLCKPLSWK--CDGEDDCGDNSDETPEMCLKFQCP 3665
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Sea Lamprey
Match: tshr (thyroid stimulating hormone receptor [Source:ZFIN;Acc:ZDB-GENE-110524-4]) HSP 1 Score: 91.6633 bits (226), Expect = 9.336e-19 Identity = 80/293 (27.30%), Postives = 144/293 (49.15%), Query Frame = 1
Query: 4840 TCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYT 5718
TC K D + CED++ ++ +++ +W + + +VGN+ V++ I + K + L+ NLA +D+ MGIYL+IIA D Y RS++ ++I W++ C++AG+ + +++ SV + +IT++R+ I F + + + ++ W+ S+ L + + S +CL + E Q + + +L +L +I YV I+ T R I D K AK++ LI +C PI + + F L+ L V P+NS NP+LY
Sbjct: 318 TCLPKPDAFNPCEDIMGDEFLRMSIWFVSLLAIVGNLFVLL--IILTSHYKPTVPRFLMCNLAFADLCMGIYLLIIAGFDLYSRSEFYNHAIDWQTGPGCNIAGFVSVFASELSVYTLMVITVERWHAITFAMRLDRKIRFRQALGVMAGGWLFSVVLAGMPLYGVSSYSKVS-ICL---PMEVETFVAQAYVVTVLALNVLAFVIICACYVHIYI----TVRNPNYISGNQDTKIAKRMAILIFTDFICMAPISFFAISAAFKLPLITVSHSKILVVVFYPLNSCANPFLYA 600
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003181.1 (pep scaffold:Pmarinus_7.0:GL476689:240399:277283:-1 gene:ENSPMAG00000002894.1 transcript:ENSPMAT00000003181.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 92.4337 bits (228), Expect = 9.599e-19 Identity = 91/285 (31.93%), Postives = 132/285 (46.32%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEK-DCDICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMK-YCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQ 4191
VCD DC +DE DC +V+ P N CN ++N+ + W CD DC G+DE C CA+ K CI S C+ V+DC D SDE DC C +C + C+ ++ D DC G S E + + C N + C++++N+C + C ++ D E +Q C + Q + C+ C+E YVCDG DC G DE + C+ CP N ++ N+S CL +C+G +DCS G DE C+ Q
Sbjct: 1170 VCDGEADCSGG-SDESDC-ERVQGCPAN----LYACNNSNNECLEWIYVCDGEADCSGGSDESDCERG-CADK-------KHCIRGSLYCDGVTDCADQSDETPDC-ACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESDCERVQGCTAN----QYACNNSNNECLEWIYVCDGEADCSGGSDE-SDCERVQGCTANQ----YACNNSNNECLEWIYVCDGEADCSGGSDESD--CEREGCPANL--YACNNSNNECLEWIYVCDGEADCSGGSDESDCERDQ 1426 HSP 2 Score: 82.4185 bits (202), Expect = 1.134e-15 Identity = 88/289 (30.45%), Postives = 134/289 (46.37%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEK-DCDICNG--FKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMK-YCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPT 4200
VCD DC +DE DC +V+ N++ CN ++N+ + W CD DC G+DE C CA+ K CI S C+ ++DC D SDE DC C +C + C+ ++ D DC G S E + + C N + C++++N+C + C ++ D E +Q C + Q + C+ C+E YVCDG DC G DE + C+ ++ C N ++ N+S CL +C+G +DCS G DE C+ C
Sbjct: 1526 VCDGEADCSGG-SDESDC-ERVQGCTANQY----ACNNSNNECLEWIYVCDGEADCSGGSDESDCERG-CADK-------KHCIRGSLYCDGLTDCADQSDETPDC-ACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESDCERVQGCTAN----QYACNNSNNECLEWIYVCDGEADCSGGSDE-SDCERVQGCTANQ----YACNNSNNECLEWIYVCDGEADCSGGSDESD--CERVQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESNCEREGCTA 1786 HSP 3 Score: 70.0922 bits (170), Expect = 5.229e-12 Identity = 88/322 (27.33%), Postives = 129/322 (40.06%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCD----ICNGFKCLSS--LCIASMFEMDDIVDCPGSSMED-------EIISKEDCYKN--------------------PAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKY--------CIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCD 4182
VCD DC +DE DC +V+ N++ CN ++N+ + W CD DC G+DE C + ++ C N +C++ C+ +DC SDE DC N + C +S C+ ++ D DC G S E E K C + C++ C SNN CL C +C Q C+ +Y C+E YVCDG DC G DE + C+ ++ CP N ++ N+S CL +C+G +DCS G DE C+
Sbjct: 923 VCDGEADCSGG-SDESDC-ERVQGCTANQY----ACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCAREGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERGMFECADKRHCIRGSLYCDGVTDCADQSDETPDCACRSDQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCARVQ-----GCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESD--CERVQGCPANL--YACNNSNNECLEWIYVCDGEADCSGGSDESDCE 1229 HSP 4 Score: 69.707 bits (169), Expect = 6.788e-12 Identity = 88/317 (27.76%), Postives = 133/317 (41.96%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDICNG-----FKCLSS--LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQ------SNNTCLYHSNNLLSRDACPLLEHLQNCI--SFQCPGLFQCSKMK------------------YCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQ 4191
VCD DC +DE DC E Q+ CN ++N+ + W CD DC G+DE C + ++ C N +C++ C+ +DC SDE DC+ G + C +S C+ ++ D DC G S E + + C N + C++++N+C + C S+ C +H CI S C GL C+ C+ YVCDG DC G DE + C+ ++ C N ++ N+S CL +C+G +DCS G DE C+ Q
Sbjct: 1406 VCDGEADCSGG-SDESDC----------ERDQY-ACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTAN----QYACNNSNNECLEWIYVCDGEADCSGGSDESDCERGCADKKH---CIRGSLYCDGLTDCADQSDETPDCACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESD--CERVQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQ 1699 HSP 5 Score: 69.707 bits (169), Expect = 7.200e-12 Identity = 88/317 (27.76%), Postives = 136/317 (42.90%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDICNG-----FKCLSS--LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQ------SNNTCLYHSNNLLSRDACPLLEHLQNCI--SFQCPGLFQCSKMK------------------YCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQ 4191
VCD DC +DE DC +V+ N++ CN ++N+ + W CD DC G+DE C R ++ C N +C++ C+ +DC SDE DC+ G + C +S C+ ++ D DC G S E + + C N + C++++N+C + C S+ C +H CI S C G+ C+ C+ YVCDG DC G DE + C+ ++ C N ++ N+S CL +C+G +DCS G DE C+ Q
Sbjct: 688 VCDGEADCSGG-SDESDC-ERVQGCTANQY----ACNNSNNECLEWIYVCDGEADCSGGSDESDCE--RGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTAN----QYACNNSNNECLEWIYVCDGEADCSGGSDESDCARECADKKH---CIRGSLYCDGVTDCADQSDETPDCACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESD--CERVQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQ 985 HSP 6 Score: 68.9366 bits (167), Expect = 1.424e-11 Identity = 86/323 (26.63%), Postives = 136/323 (42.11%), Query Frame = 1
Query: 3352 CDFHIDCLISENDEKDCIVKV-KSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDICNG-----FKCLSS--LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSL------CSSTSNKCFQSNNT--CLYHSNNLLSRDACPL--------------------LEHLQNCISFQCPGLFQCSKM-KYCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPT 4200
CD DC ++ DC + + N++ CN ++N+ + W CD DC G+DE C+ + ++ C N +C++ C+ +DC SDE DC+ G + C +S C+ ++ D DC G S E DC + A+ K C ++ QS+ T C S+ + + L E +Q C + Q + C+ C+E YVCDG DC G DE + C+ ++ C N ++ N+S CL +C+G +DCS G DE C+ CP
Sbjct: 1084 CDGVTDCADQSDETPDCACRSDQGCTANQY----ACNNSNNECLEWIYVCDGEADCSGGSDESDCARVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCPANLYACNNSNNECLEWIYVCDGEADCSGGSDE------SDCERGCADKKHCIRGSLYCDGVTDCADQSDETPDCACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESDCERVQGCTANQ----YACNNSNNECLEWIYVCDGEADCSGGSDESD--CERVQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESDCEREGCPA 1388 HSP 7 Score: 68.5514 bits (166), Expect = 1.657e-11 Identity = 82/303 (27.06%), Postives = 122/303 (40.26%), Query Frame = 1
Query: 3352 CDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEK-DCDICNG--FKCLSSLCIASMFEMDDIVDCPGSSME--------DEIISKEDCYKNPAEIKSL------------CSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKM-KYCIELHYVCDGTKDCPDGEDED--EESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCD 4182
CD DC ++ DC + + Q CN ++N+ + W CD DC G+DE C E ++ K CI S C+ V+DC D SDE DC C +C + C+ ++ D DC G S E + K+ C + + C+ S++ N +CL ++ + C NCI + C+ C+E YVCDG DC G DE E C + ++ N+S CL +C+G +DCS G DE C+
Sbjct: 239 CDGVTDCADQSDETPDCACRSDQQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESDC------ERGMFEHHKKHCIRGSLYCDGVTDCADESDETPDC-ACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESDCERGMFEHCADKKHCIRGSLYCDGVTDCADQSDETPDCACRSDQIRCGNGSCLGIASLCDGVNDCGDFTDESNCIQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERGCTANQ-------YACNNSNNECLEWIYVCDGEADCSGGSDESDCE 525 HSP 8 Score: 67.0106 bits (162), Expect = 4.473e-11 Identity = 79/286 (27.62%), Postives = 122/286 (42.66%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDICNG-----FKCLSS--LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKM-KYCIELHYVCDGTKDCPDGEDED--EESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE 4170
VCD DC +DE DC + + P N CN ++N+ + W CD DC G+DE C E ++ C N +C++ C+ +DC SDE DC+ G + C +S C+ ++ D DC G S E + + C N + C++++N+C + C ++ D E +Q C + Q + C+ C+E YVCDG DC G DE E C K C+ C+G++DC++ DE
Sbjct: 1365 VCDGEADCSGG-SDESDC--EREGCPAN----LYACNNSNNECLEWIYVCDGEADCSGGSDESDC------ERDQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTAN----QYACNNSNNECLEWIYVCDGEADCSGGSDE-SDCERVQGCTANQ----YACNNSNNECLEWIYVCDGEADCSGGSDESDCERGCADKK---------------HCIRGSLYCDGLTDCADQSDE 1613 HSP 9 Score: 60.077 bits (144), Expect = 6.415e-9 Identity = 81/294 (27.55%), Postives = 125/294 (42.52%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNG-----FKCLSS--LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKM-KYCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTK 4203
VCD DC +DE +C K I + YCD + DC + +DE R ++ +C N C+ C+ +DC SDE DC+ G + C +S C+ ++ D DC G S E DC + + C++++N+C + C ++ D E +Q C + Q + C+ C+E YVCDG DC G DE + C+ ++ C N ++ N+S CL +C+G +DCS G DE C +C K
Sbjct: 620 VCDGEADCSGG-SDESNCERGADKK----------------HCIRGSLYCDGVTDCADQSDETPDCACRSDQI---RCGNGSCLGWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDE------SDCERGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDE-SDCERVQGCTANQ----YACNNSNNECLEWIYVCDGEADCSGGSDESD--CERVQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESDC-ARECADK 877 HSP 10 Score: 52.7582 bits (125), Expect = 9.668e-7 Identity = 72/279 (25.81%), Postives = 106/279 (37.99%), Query Frame = 1
Query: 3352 CDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSL-CIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEE-SCKT--LKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDE 4170
CD DC ++ DC + + + C I W CD DC G+DE C C ++ C N +C++ C+ +DC SDE DC+ C CI D + DC S E DC +I+ N +CL C NC G+F+C+ K+CI CDG DC D DE + +C++ ++C N C H C+ C+G++DC++ DE
Sbjct: 8 CDGVTDCADQSDETPDCACR---------EDQIRCRNGSCLGIEWIYVCDGEADCSGGSDESDCEREGCT-ANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERGMFEHCADKKHCIRGSLYCDGVTDCADQSDE-----TPDCACRSDQIRC-----------GNGSCLGWIYVCDGEADCSGGSDESNCE----RGMFECADKKHCIRGSLYCDGVTDCADQSDETPDCACRSDQIRCG-NGSCLDKKH----CIRGSLYCDGVTDCADQSDE 251 HSP 11 Score: 51.9878 bits (123), Expect = 1.629e-6 Identity = 87/339 (25.66%), Postives = 137/339 (40.41%), Query Frame = 1
Query: 3334 IGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDIC---NGFKCLSS--LCIASMFEMDDIVDCPGSSMED-------EIISKEDCYK------------NPAEIKSLCSSTSNKCFQSNNTCL---------YHSNNLLSRDACPLLEHLQNCI--SFQCPGLFQCSKMK------------------YCIELHYVCDGTKDCPDGEDEDEESCKTLK-CPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCD 4182
+GI +CD DC DE +CI + N++ CN ++N+ + W CD DC G+DE C R ++ C N +C++ C+ +DC SDE DC+ N + C +S C+ ++ D DC G S E E K+ C + + ++ C+ S++ N +CL + C ++CI S C G+ C+ C+ YVCDG DC G DE + C+ ++ C N ++ N+S CL +C+G +DCS G DE C+
Sbjct: 423 LGIASLCDGVNDCGDF-TDESNCI---QGCTANQY----ACNNSNNECLEWIYVCDGEADCSGGSDESDCE--RGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERGCTENQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERGMFECADKKHCIRGSLYCDGVTDCADESDETPDCACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESNCERGADKKHCIRGSLYCDGVTDCADQSDETPDCACRSDQIRCGNGSCLGWIYVCDGEADCSGGSDESD--CERVQGCTANQ--YACNNSNNECLEWIYVCDGEADCSGGSDESDCE 747 HSP 12 Score: 51.6026 bits (122), Expect = 2.319e-6 Identity = 50/175 (28.57%), Postives = 82/175 (46.86%), Query Frame = 1
Query: 3349 VCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNK--QCIDVSKRCNKVSDCIDNSDEKDCDICNG-----FKCLSS--LCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQ 3846
VCD DC +DE DC +V+ N++ CN ++N+ + W CD DC G+DE C + ++ C N +C++ C+ +DC SDE DC+ G + C +S C+ ++ D DC G S E +E C N + C++++N+C +
Sbjct: 1637 VCDGEADCSGG-SDESDC-ERVQGCTANQY----ACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESDCERVQGCTANQYACNNSNNECLEWIYVCDGEADCSGGSDESN-CEREGCTAN----QYACNNSNNECLE 1800
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Nematostella
Match: EDO34405 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPG8]) HSP 1 Score: 138.272 bits (347), Expect = 1.698e-35 Identity = 95/307 (30.94%), Postives = 162/307 (52.77%), Query Frame = 1
Query: 4843 CSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICII--FRTYRQHASKIKYLVFLVWFVWVIS----IALFQHFYHSKMDEC--ESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQL-TWLTAVLMPINSSINPYLYTFSNVNL 5736
C + D S+C+DL+ NK +QV VW +G+ L+GN+ VI WR++ + + T +L+ NLA+SD+ MG+YL+IIA D ++ +Y ++ + W++ + C AG + +S++ SV + LIT DR ICI+ F+ R + L ++W + I+ + +FY S D SG+CL K + + + + I+ Y+ +F+ R M + AKKL+ +I CW+P+I++ LS + + Q+ W+ ++P+NSSINP LYTFS ++
Sbjct: 18 CVSPRDGFSSCDDLMKNKGLQVAVWVLGLMALIGNVFVIFWRVTDGQSNQ--THSLLLTNLAVSDLLMGVYLMIIAITDAKWQGEYFKHDVTWRAGIGCQFAGIISMLSSEVSVFILALITADRLICIVFPFKFRRLTRKRAMILCVIIWTLGAIASLLPVTGISYFYDSDGDFGFYGRSGVCLPLQLSKSRPAGWEYSVAFFIGLNSIAFIFILVAYMTMFWTVKRVSGAVRSTSMNKESAMAKKLIFIISTDFCCWMPVIVIGILSLTGAFYDPNKQVYAWIAVFVLPVNSSINPILYTFSTTHV 322
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Nematostella
Match: EDO32738 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU96]) HSP 1 Score: 141.739 bits (356), Expect = 5.981e-35 Identity = 97/323 (30.03%), Postives = 164/323 (50.77%), Query Frame = 1
Query: 4435 LSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICC-SLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFR----------TYRQHASKIKYLVFLVWFVWVISIALFQHFYHSKMDECESSGLCL 5370
LS N ++I+ S F L L+ L + NP+T IE+ FH L L +N++N D+ +I ++F G++ L L ++ N N + ++L ++ + + ++ CC + S CSA D+ S+C DL+AN+++ + +W +G L GN V+IWR+ K + +L++NLA+SD MG+Y+++I S DKYYR +Y + WK S C G+ +TVS ++SV + +T+DR++ I+ Y+ S I L F++ F+ + I F+ FY SG+CL
Sbjct: 25 LSNNLLKIIRSNTFDGLFSLEHLKLDGNPVTLIETAGFHGL-------------------------RKLRELNLTNLDLETIHDNAFSGMDTLQRLYLDRNANLYRLPLDIFENLPLEFLKADRFEFCCIAKLSGANCSAPKDLFSSCNDLMANETLGISIWILGCIALFGNGFVLIWRLKT--KSESRVHSLLLLNLALSDFMMGVYMVVIGSVDKYYRGKYFIYNEEWKRSHLCQFCGFLSTVSCEASVFILTTMTVDRYVAIVHPLRNLCLKISGAYKALIS-IWLLAFILAFLPLTGIKYFKDFYG-------RSGVCL 312
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Nematostella
Match: EDO33378 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSB7]) HSP 1 Score: 134.806 bits (338), Expect = 1.419e-34 Identity = 100/305 (32.79%), Postives = 165/305 (54.10%), Query Frame = 1
Query: 4840 TCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVW-------VISIALFQHFYHSK--MDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFSN 5727
TC + SD S+CEDL+ NK I+V VW +G+ L+GN+ VI+WRI K+ +L++NLA SD+ MG+YL++IA D ++ +Y ++ I W+S + C VAG + +S++ SV + +IT DR CI+F ++ K + F VW V+ +A ++FY K S +CL+ + + + ++ + L+ ++ Y+ +F+ R R M + AKKL+ +IL CW+P+I++ LS L++ W+ ++P+NSSINP LYTFS
Sbjct: 1 TCESPSDDFSSCEDLMKNKYIRVCVWILGLMALLGNMSVILWRI--LDKETNQIHSMLLLNLAFSDLLMGVYLLLIAIMDTKWQGEYFKHDIEWRSGIGCQVAGILSMLSSEVSVLILTVITADRLACIVF-PFKFKRLKKRSATFTCTVVWIFSLVISVLPVAGLEYFYDKKGYFGFYGRSAVCLALQLSEDRPAGWEYAVVIFIGVNLVAFMFMLFAYIAMFWTVKRVSRAVRSTSMSKESAMAKKLIFIILTDFCCWMPVIVIGILSLTGLLLIQV--YVWIAVFVLPVNSSINPILYTFST 300
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Nematostella
Match: EDO38772 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBV6]) HSP 1 Score: 130.568 bits (327), Expect = 7.930e-33 Identity = 100/313 (31.95%), Postives = 164/313 (52.40%), Query Frame = 1
Query: 4843 CSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIF--RTYRQHASKIKYLVFLVW----FVWVISIALFQHFYHSKMDEC--ESSGLCLSY-------FSFKYECSF--KQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLT--WLTAVLMPINSSINPYLYTFS 5724
C A +D S+C+DL+ +K++QV +W +GI GNI V+ +R+ V + + +L+ NLAISD MG+YL+IIA D ++ +Y + + W+ S C AG + +S++ SV + +ITLDRFICI+F R R K L LVW + ++ I ++FY +++ S +CL ++Y SF NF + I+ Y+ IF+ ++ G + A+KL+ +I+ CW+P+I + LS ++ + +L W+ ++P+NSSINP LYTFS
Sbjct: 18 CKAPNDEFSSCDDLMKDKTLQVCIWVLGISAFFGNIFVVAYRLVVREDNR--VQSLLLTNLAISDFMMGLYLLIIAFKDVQWQGEYFKYDLSWRVSGLCQFAGALSMISSEVSVLMLTIITLDRFICIVFPMRFGRLGLKKAFALCILVWTFGMLISIVPILGIEYFYDEQLEIGFYGRSSVCLPLQLSSDRPAGWEYSVSFFIGLNF---------MAFMFILVAYIAIFWTVRKSSAAVGSRNAKKEAALARKLMAIIMTDFCCWMPVIFIGILSLTGDF-RDPQKLAYVWIAVFVLPVNSSINPILYTFS 318
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Nematostella
Match: EDO34445 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBV6]) HSP 1 Score: 130.568 bits (327), Expect = 7.930e-33 Identity = 100/313 (31.95%), Postives = 164/313 (52.40%), Query Frame = 1
Query: 4843 CSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIF--RTYRQHASKIKYLVFLVW----FVWVISIALFQHFYHSKMDEC--ESSGLCLSY-------FSFKYECSF--KQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLT--WLTAVLMPINSSINPYLYTFS 5724
C A +D S+C+DL+ +K++QV +W +GI GNI V+ +R+ V + + +L+ NLAISD MG+YL+IIA D ++ +Y + + W+ S C AG + +S++ SV + +ITLDRFICI+F R R K L LVW + ++ I ++FY +++ S +CL ++Y SF NF + I+ Y+ IF+ ++ G + A+KL+ +I+ CW+P+I + LS ++ + +L W+ ++P+NSSINP LYTFS
Sbjct: 18 CKAPNDEFSSCDDLMKDKTLQVCIWVLGISAFFGNIFVVAYRLVVREDNR--VQSLLLTNLAISDFMMGLYLLIIAFKDVQWQGEYFKYDLSWRVSGLCQFAGALSMISSEVSVLMLTIITLDRFICIVFPMRFGRLGLKKAFALCILVWTFGMLISIVPILGIEYFYDEQLEIGFYGRSSVCLPLQLSSDRPAGWEYSVSFFIGLNF---------MAFMFILVAYIAIFWTVRKSSAAVGSRNAKKEAALARKLMAIIMTDFCCWMPVIFIGILSLTGDF-RDPQKLAYVWIAVFVLPVNSSINPILYTFS 318
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Medaka
Match: rxfp2a (relaxin family peptide receptor 2 [Source:NCBI gene;Acc:101168141]) HSP 1 Score: 138.272 bits (347), Expect = 1.295e-32 Identity = 136/509 (26.72%), Postives = 239/509 (46.95%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF----------------------QFLK---QLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVI-NVNKNDIAMSG-KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFL--VWFVWVISIA-------LFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAG--RILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
LS G+F L +L + N + L F GL +L YL + ++ LP +F LK +L++L + DN I + F +L + + L S ++ E+ TF +S++L+ +N+S N + I F + +L +L + + DI P+++L+ ++Y V++ C +C +D S+ EDLLAN ++V VW M GN++VI R S+ R + + + I L +D MG+YL + D +R QY N++ W SV C G+ A +S++ SV + +TL++F+ I+F K++ +V L +W + I A +F ++Y +G+C S + E + +S + L + L FL++T Y +F++ +T A R + D A + ++ LCWIPI ++ LS + + +W+ ++PINS++NP LYT +
Sbjct: 177 LSPGVFRDLNQLEWLMLDHNPLGGLSPDTFIGLHSLMYLSMVHTSLQQLPHPSFCQHMPALDYLDLEGNHIETLNYSILKTCSKLEVLLLMDNKIRRVPENTFQSLWKLAELNLSSNRIKELPKNTFKNLSKSLLKLNISYNTLLRIHPAHFSHLTHLQSLAVEGIEIPDIQTKMFLPMRNLS--HIYFKVFQYCSYAPHVRSCKPNTDGISSFEDLLANMVLRVSVWVMAFITCFGNLLVIGMR-SLIRAEN-NLHAVCIKVLCCADCLMGVYLFFVGVFDVKFRGQYNRNALQWMESVECRTIGFLAMLSSEVSVLLLTYLTLEKFLVIVFPFSNLRPGKLQTVVILSSIWLMGFIIAAVPLMNEEIFGNYY-------GRNGVCFPLHSDRQEKPTAKGYSTGIFLGLNLVAFLVITMSYSSMFYNIYKTGINATDLRSRLHKDVAVANRFFFIVFSDALCWIPIFLVKVLSLLEVEIPGTIS-SWVVIFVLPINSALNPILYTLT 673
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Medaka
Match: rxfp2a (relaxin family peptide receptor 2 [Source:NCBI gene;Acc:101168141]) HSP 1 Score: 137.887 bits (346), Expect = 1.787e-32 Identity = 136/509 (26.72%), Postives = 239/509 (46.95%), Query Frame = 1
Query: 4315 LSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF----------------------QFLK---QLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVI-NVNKNDIAMSG-KPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFL--VWFVWVISIA-------LFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAG--RILMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
LS G+F L +L + N + L F GL +L YL + ++ LP +F LK +L++L + DN I + F +L + + L S ++ E+ TF +S++L+ +N+S N + I F + +L +L + + DI P+++L+ ++Y V++ C +C +D S+ EDLLAN ++V VW M GN++VI R S+ R + + + I L +D MG+YL + D +R QY N++ W SV C G+ A +S++ SV + +TL++F+ I+F K++ +V L +W + I A +F ++Y +G+C S + E + +S + L + L FL++T Y +F++ +T A R + D A + ++ LCWIPI ++ LS + + +W+ ++PINS++NP LYT +
Sbjct: 199 LSPGVFRDLNQLEWLMLDHNPLGGLSPDTFIGLHSLMYLSMVHTSLQQLPHPSFCQHMPALDYLDLEGNHIETLNYSILKTCSKLEVLLLMDNKIRRVPENTFQSLWKLAELNLSSNRIKELPKNTFKNLSKSLLKLNISYNTLLRIHPAHFSHLTHLQSLAVEGIEIPDIQTKMFLPMRNLS--HIYFKVFQYCSYAPHVRSCKPNTDGISSFEDLLANMVLRVSVWVMAFITCFGNLLVIGMR-SLIRAEN-NLHAVCIKVLCCADCLMGVYLFFVGVFDVKFRGQYNRNALQWMESVECRTIGFLAMLSSEVSVLLLTYLTLEKFLVIVFPFSNLRPGKLQTVVILSSIWLMGFIIAAVPLMNEEIFGNYY-------GRNGVCFPLHSDRQEKPTAKGYSTGIFLGLNLVAFLVITMSYSSMFYNIYKTGINATDLRSRLHKDVAVANRFFFIVFSDALCWIPIFLVKVLSLLEVEIPGTIS-SWVVIFVLPINSALNPILYTLT 695
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Medaka
Match: rxfp1 (relaxin family peptide receptor 1 [Source:NCBI gene;Acc:101159175]) HSP 1 Score: 125.946 bits (315), Expect = 1.107e-28 Identity = 161/651 (24.73%), Postives = 276/651 (42.40%), Query Frame = 1
Query: 3940 CP-GLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFS----QFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAF-----------------------QFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNV---------------YTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISI----------ALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVT-GYVLIFFHSCRTRRTAGRI--LMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
CP G F C + C+ C+G DC G DEE+C + W H F D G+ + C + P +C+C ++ C + LT S +F+ F YL Y N I L G+F L L +LIL N I + + F Q + +L LD+ N I I + +FH+ +T + L ++ I++ F + L +++SNN + +I + F+ + +L L I+ N P+ L V + Y ++ C +C +D S+ E+LLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I S D +R +Y ++ W S C + G A +S + SV + +TL+++ICI++ ++ + V ++ +WV + LF++FY ++G+C S + E + +SI++ L + L FLI+ Y +F++ RT + + + AK+ ++++ LCWIPI +L LS + + +W+ ++PINS++NP LYT +
Sbjct: 29 CPLGYFPCGNLTVCLPQLLHCNGIDDC--GNQADEENCG------DNNGWP--HLF-------DNYFGMPSHNTQTKSNLCLLGAVPERCQCLDLELDC-------------DNKNLTTCTKCPFLPHSNLFNVCLLSFRYLSY-----NKITVLKPGVFRDLHKLEWLILENNNIHQISAMTFSGLNSLVLLVLLNNSLTKLADICQEMPRLNWLDLEGNLIEAIGNVSFHSCSMLTVLVLQRNRINYIHEQAF-SVLRKLGELDLSNNRLVAIPRKLFILLEDLLQLNISYN---------PIMELQVDHFDRLHKLKSLLFFSWFYFKKFQYCGYAPHVRSCKPNTDGISSFENLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLCCADGLMGVYLFMIGSYDLKFRGEYNRHAQSWMDSTQCQIIGALAMLSTEVSVLLLTYLTLEKYICIVY-PFQYLTPGRRRTVTILTSIWVFGLIVAFLPLACKGLFRNFY-------GTNGVCFPLHSEQPETPWAHVYSIVIFLGLNLVAFLIIVFSYASMFYNIQRTGTQTTKFSNHIKKEVTIAKRFFSIVITDSLCWIPIFVLKILSLLQVEIPGTIS-SWVVIFILPINSALNPILYTLT 623
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Medaka
Match: rxfp1 (relaxin family peptide receptor 1 [Source:NCBI gene;Acc:101159175]) HSP 1 Score: 107.071 bits (266), Expect = 7.305e-23 Identity = 118/442 (26.70%), Postives = 211/442 (47.74%), Query Frame = 1
Query: 4435 LSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVISI----------ALFQHFYHSKMDECESSGLCLSYFSFKYECSFKQNFSXXXXXXXXXXXXXXVTGYVLIFFHSCRTRRTAGRI--LMLNDYKSAKKXXXXXXXXXXXXXXXXXXSFLSRFSNYLMESDQLTWLTAVLMPINSSINPYLYTFS 5724
L N I + + +F L ++ + N I I AF L + +L + +L I F+ + E+L+ N+S N I + D F ++ L ++ N +PLK+LT Y ++ C +C +D S+ E+LLAN ++VFVW + GNI VI R + + K + I++L +D MG+YL +I S D +R +Y ++ W S C + G A +S + SV + +TL+++ICI++ ++ + V ++ +WV + LF++FY ++G+C S + E + +SI++ L L+ +IV Y +F++ RT + + + AK+ ++++ LCWIPI +L LS + + +W+ ++PINS++NP LYT +
Sbjct: 220 LEGNLIEAIGNVSFHSCSMLTVV-LQRNRINYIHEQAFSVLRKLGE-DLSNNRLVAIPRKLFI-LLEDLLQ-NISYNPIMELQVDHFDRLHKLKSIEGIEIGNIQRRMFEPLKYLTH---YFKKFQYCGYAPHVRSCKPNTDGISSFENLLANIVLRVFVWVVSATTCFGNIFVICMRSYIRSENKLHA--MCIISLCGADGLMGVYLFMIGSYDLKFRGEYNRHAQSWMDSTQCQIIGALAMLSTEVSVLLLTYLTLEKYICIVY-PFQYLTPGRRRTVTILTSIWVFGLIVAFLPLACKGLFRNFY-------GTNGVCFPLHSEQPETPWAHVYSIVIFLLNLVAFLIIVFSYASMFYNIQRTGTQTTKFSNHIKKEVTIAKRFFSIVITDSLCWIPIFVLKILSLLQVEIPGTIS-SWVVIFILPINSALNPILYTLT 643
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Medaka
Match: fshr (follicle-stimulating hormone receptor [Source:NCBI gene;Acc:100529182]) HSP 1 Score: 105.145 bits (261), Expect = 2.904e-22 Identity = 99/412 (24.03%), Postives = 178/412 (43.20%), Query Frame = 1
Query: 4270 PKTRTLTIVQTHSLE-LSSGIFSQFIYLGYLNISDNSIL-ELYNGLFDGLFNLRYLILSKNYIRILP------SYAFQFLKQLQILDITDN-PITTIESFAFHNLIS--ITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKP------LKHLTVKNVY--------------------------------------------------TNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLITLDRFICIIFRTYRQHASKIKYLVFLVWFVWVIS 5304
P+ ++I Q +L+ + + FS L + I+ + L ++ F L LR LI++ +RI P S +F+ D+ DN I I + AF L + + I L + E+ F G + +S+ N + IS D+FVG + L L I+ + A+S P LK L ++ Y T+ Y+ CS ++ TCS D + CED+++ ++V +W + + L+GN +V++ + + + K + L+ +LA +D+ MGIYLIIIA+ D Y +I W++ + CS AG+F +++ SV + IT++R+ I ++++ ++ W+ S
Sbjct: 95 PRLFDVSISQNMALKKIGAFAFSNLPALTEIEITKSRHLTSIHPDAFRSLVGLRRLIITNTGLRIFPDLSKIHSAVHRFM-----FDLQDNIHIQAIPANAFRGLCTQTVEEIRLTRNGIREVASDAFNGTKMHRLSLK-GNKQLTHISGDAFVGSSELVVLDIS----ETAISSLPDNIISGLKKLLAESAYHLKILPPPQQFAKLRLAKLTYPSHCCAFKNKPRSRSKWSPLCSHPMAKYITDFYRDHCSNSTSITCSPTPDNLNPCEDIMSPVPLRVLIWIISVLALLGNTVVLV--VLLGSRSKLTVPRFLMCHLAFADLCMGIYLIIIATIDMLTHGHYYNYAIDWQTGLGCSAAGFFTVFASELSVFTLTAITVERWHTITHALRLDRKLRMRHACVIMAAGWIFS 494
BLAST of G-protein coupled receptor GRL101 vs. Planmine SMEST
Match: SMESG000017864.1 (SMESG000017864.1) HSP 1 Score: 2109.34 bits (5464), Expect = 0.000e+0 Identity = 1044/1053 (99.15%), Postives = 1048/1053 (99.53%), Query Frame = 2
Query: 89 MDNTKYLIIDSPYNTTSKQYYANLNCTLQLINYNHSGILIEIVYLLIEFSPGCLYRDYLSITFDNETKPSYYCGNIYSKYVTTNSSVVTFQFRSDPIIENTGFMLSVNLLENNLHKTDFCQQFMFITNYKILISSKETKGETIDCSGTIFSTTGLGVVFEIKKLDTKSYYQSCHEEYIKFDYFDYFLNETVEKYFCGFIDKNIGEPILVAYGTEIKFSAKFQMSGELNIEYRPIIFPENVYSILSKVSHIELSKLPESLTIDLNNKLIFAENFSYKHCLFAFKIQAPLGMGINVVFSQYNVQLYRAYFMENFINQVNFLTIREMKILNTSEWSTESFQITLVIRYFNCNSNLEKFNLRFTLKQSNLMIDYPLINTILLGEKWWEWINKENYKRTIQITNYINPPKQDMTFYYIRINKLILFNQNHQDYSCNQTGLYLLYDNSSKRFGPFCHSTDFIPLEKLPTTIAYYYFPTDEIKFGSENKTNSILEILLEFYPCKISSLQTSYIVQLQKCLIFYQPVTNQPINSSNKVILIRANENVTLFKMHTRLSMNFEQNIISLNKPIHLPIIQSNLCYFGFVLSQNVMNSENYLMLLEINSNCSYIPHNWSIHLELASRRRIECNSVNNIPSGIISLNSSTLSNVTYLDLVIPICTWHFKRPNYPTVYNSVNMLLLEIDVFNLDCKKYTFHMFEKIGDTVHKLNLCDNVKHGDLIYSKSDGDIXXXXXXXXXXXXWNDLVPQYHFDDLVIFFKYVSVNFTPVKMNCEDGWWKYDKSCYKFEKRQLDISWNDSLEICKTYNSKLVQISNRNEMEFLRNISLSRWLSEIYSNTSVVIFIGLRNHITMPKDYDYVEDLYRLTWTKDEPLTYHEWFQRSPSKTYAFLSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPLYWSNWVKIPCESKISSASFICQKPQYIKNSKYYFTKELEKINDMDGARCSENWFFIEKRCLMLIKINDFEKSKSYFCELKNSTLAFITGKEILRTTMILRQFLTVNKDTEMYYQDKSGEKIVPSISYSFKEEPRKGYVFAK 3247
MDNTKYLIIDSPYNTTSKQYYANLNCTLQLINYN+SGILIEIVYLLIEFSPGCLYRDYLSITFDNETKPSYYCGNIYSKYVTTNSSVVTFQFRSDPIIENTGFMLSVNLLENNLHKTDFCQ FMFITNYKILISSKET GETIDCSGTIFSTTGLGVVFEIKKLDTKSYYQSCHEEYIKFDYFDYFLNETVEKYFCGFIDKNIGEPILVAYGTEIKFSAKFQMSGELNIEYRPIIFPENVYSILSKVSHIELSKLPESLTIDLNNKLIFAENFSYKHCLFAFKIQAPLGMGINVVFSQYNVQLYRAYFMENFINQVNFLTIREMKILNTSEWSTESFQITLVIRYFNCNSNLEKFNLRFTLKQSNLMIDYPLINTILLGEKWWEWINKENYKRTIQITNYINPPKQDMTFYYIRINKLILFNQNHQDYSCNQTGLYLLYDNSSKRFGPFCHSTDFIPLEKLPTTIAYYYFPTD+IKFGSENKTNSILEILLEFYPCKISSLQTSYIVQLQKCLIFYQPVTNQPINSSNKVILIRANENVTLFKMHTRLSMNFEQNIISLNKPIHLPIIQSNLCYFGFVLSQNVMNSENYLMLLEINSNCSYIPHNWSIHLELASRRRIECNSVNNIPSGIISLNSSTLSNVTYLDLVIPICTWHFKRPNYPTVYNSVNMLLLEIDVFNLDCKKYTFHMFEKIGDTVHKLNLCDNVKHGDLIYSKSDGDIYLSLKSNYLSNSWNDLVPQYHFDDLVIFFKYVSVNFTPVKMNCEDGWWKYDKSCYKFEKRQLDISWNDSLEICKTYNSKLVQI NRNEMEFLRNISLSRWLSEIYSNTSVVIFIGLRNHITMPK+YDYVEDLYRLTWTKDEPLTYHEWFQRSPSKTYAFLSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPLYWSNWVKIPCESKISSASFICQKPQYIKNSKYYFTKELEKINDMDGARCSENWFFIEKRCLMLIKINDFEKSKSYFCELKNSTLAFITGKEILRTTMILRQFLTVNKDTEMYYQDKSGEKIVPSISYSFKEEPRKGYV +
Sbjct: 1 MDNTKYLIIDSPYNTTSKQYYANLNCTLQLINYNNSGILIEIVYLLIEFSPGCLYRDYLSITFDNETKPSYYCGNIYSKYVTTNSSVVTFQFRSDPIIENTGFMLSVNLLENNLHKTDFCQHFMFITNYKILISSKETIGETIDCSGTIFSTTGLGVVFEIKKLDTKSYYQSCHEEYIKFDYFDYFLNETVEKYFCGFIDKNIGEPILVAYGTEIKFSAKFQMSGELNIEYRPIIFPENVYSILSKVSHIELSKLPESLTIDLNNKLIFAENFSYKHCLFAFKIQAPLGMGINVVFSQYNVQLYRAYFMENFINQVNFLTIREMKILNTSEWSTESFQITLVIRYFNCNSNLEKFNLRFTLKQSNLMIDYPLINTILLGEKWWEWINKENYKRTIQITNYINPPKQDMTFYYIRINKLILFNQNHQDYSCNQTGLYLLYDNSSKRFGPFCHSTDFIPLEKLPTTIAYYYFPTDKIKFGSENKTNSILEILLEFYPCKISSLQTSYIVQLQKCLIFYQPVTNQPINSSNKVILIRANENVTLFKMHTRLSMNFEQNIISLNKPIHLPIIQSNLCYFGFVLSQNVMNSENYLMLLEINSNCSYIPHNWSIHLELASRRRIECNSVNNIPSGIISLNSSTLSNVTYLDLVIPICTWHFKRPNYPTVYNSVNMLLLEIDVFNLDCKKYTFHMFEKIGDTVHKLNLCDNVKHGDLIYSKSDGDIYLSLKSNYLSNSWNDLVPQYHFDDLVIFFKYVSVNFTPVKMNCEDGWWKYDKSCYKFEKRQLDISWNDSLEICKTYNSKLVQIGNRNEMEFLRNISLSRWLSEIYSNTSVVIFIGLRNHITMPKNYDYVEDLYRLTWTKDEPLTYHEWFQRSPSKTYAFLSNSLKGIINSILLQPTVEKNGHFCTAMMMQHPLYWSNWVKIPCESKISSASFICQKPQYIKNSKYYFTKELEKINDMDGARCSENWFFIEKRCLMLIKINDFEKSKSYFCELKNSTLAFITGKEILRTTMILRQFLTVNKDTEMYYQDKSGEKIVPSISYSFKEEPRKGYVICE 1053 HSP 2 Score: 1301.58 bits (3367), Expect = 0.000e+0 Identity = 653/655 (99.69%), Postives = 653/655 (99.69%), Query Frame = 1
Query: 3238 ICERSSQTIKSLINASKEQINGFWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYXXXXXXXXXXXXXXXXPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIGLI 5202
ICERSSQTIKSLINASKEQINGFWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYQILCQSSLSINISILNPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFI I
Sbjct: 1051 ICERSSQTIKSLINASKEQINGFWCGEIDAYWIGIDKVCDFHIDCLISENDEKDCIVKVKSKPMNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICNGFKCLSSLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTLKCPYNFRCWSFNHSFLLCLNHKDLCNGISDCSEGEDEQYCDIFQCPTKCKCFGYQILCQSSLSINISILNPKTRTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDIAMSGKPLKHLTVKNVYTNVYKICCSLASYGTCSAKSDISSNCEDLLANKSIQVFVWTMGICGLVGNIMVIIWRISVARKKKFSTEDILIVNLAISDMFMGIYLIIIASADKYYRSQYIENSIYWKSSVACSVAGYFATVSNQSSVTFIEHI 1705
BLAST of G-protein coupled receptor GRL101 vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 63.5438 bits (153), Expect = 1.967e-9 Identity = 69/226 (30.53%), Postives = 112/226 (49.56%), Query Frame = 1
Query: 3547 YSRCAEL---TEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICN--GFKCLS-SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTL-KCPYNFRCWSFNHSFLL-----CLNHKDLCNGISDCSEGEDEQYCDIF 4188
YS C ++++C + +CI+ SK C+KV+DC ++ DEK+C++C+ F+C+S CI + D +C +S DEI C P +I +C + S C + C + + ++ + +NC Q ++C+ + CI L+ CDG DC DG DE C+ + K P C + S C+N C+G+ DC G DE+ C+IF
Sbjct: 1309 YSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNS--DEI----SCGCLPHQI--VC-NISRTCIEEKKICDGNYDCGVNDKS-----DEENCKKCQMTE-YKCNNSQICILLNNKCDGQDDCGDG--SDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517
BLAST of G-protein coupled receptor GRL101 vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 63.5438 bits (153), Expect = 1.971e-9 Identity = 69/226 (30.53%), Postives = 112/226 (49.56%), Query Frame = 1
Query: 3547 YSRCAEL---TEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICN--GFKCLS-SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTL-KCPYNFRCWSFNHSFLL-----CLNHKDLCNGISDCSEGEDEQYCDIF 4188
YS C ++++C + +CI+ SK C+KV+DC ++ DEK+C++C+ F+C+S CI + D +C +S DEI C P +I +C + S C + C + + ++ + +NC Q ++C+ + CI L+ CDG DC DG DE C+ + K P C + S C+N C+G+ DC G DE+ C+IF
Sbjct: 1309 YSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNS--DEI----SCGCLPHQI--VC-NISRTCIEEKKICDGNYDCGVNDKS-----DEENCKKCQMTE-YKCNNSQICILLNNKCDGQDDCGDG--SDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517
BLAST of G-protein coupled receptor GRL101 vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 63.5438 bits (153), Expect = 2.036e-9 Identity = 69/226 (30.53%), Postives = 112/226 (49.56%), Query Frame = 1
Query: 3547 YSRCAEL---TEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICN--GFKCLS-SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTL-KCPYNFRCWSFNHSFLL-----CLNHKDLCNGISDCSEGEDEQYCDIF 4188
YS C ++++C + +CI+ SK C+KV+DC ++ DEK+C++C+ F+C+S CI + D +C +S DEI C P +I +C + S C + C + + ++ + +NC Q ++C+ + CI L+ CDG DC DG DE C+ + K P C + S C+N C+G+ DC G DE+ C+IF
Sbjct: 1309 YSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNS--DEI----SCGCLPHQI--VC-NISRTCIEEKKICDGNYDCGVNDKS-----DEENCKKCQMTE-YKCNNSQICILLNNKCDGQDDCGDG--SDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1517
BLAST of G-protein coupled receptor GRL101 vs. Planmine SMEST
Match: SMESG000042718.1 (SMESG000042718.1) HSP 1 Score: 63.5438 bits (153), Expect = 2.123e-9 Identity = 69/226 (30.53%), Postives = 112/226 (49.56%), Query Frame = 1
Query: 3547 YSRCAEL---TEWQCQNKQCIDVSKRCNKVSDCIDNSDEKDCDICN--GFKCLS-SLCIASMFEMDDIVDCPGSSMEDEIISKEDCYKNPAEIKSLCSSTSNKCFQSNNTCLYHSNNLLSRDACPLLEHLQNCISFQCPGLFQCSKMKYCIELHYVCDGTKDCPDGEDEDEESCKTL-KCPYNFRCWSFNHSFLL-----CLNHKDLCNGISDCSEGEDEQYCDIF 4188
YS C ++++C + +CI+ SK C+KV+DC ++ DEK+C++C+ F+C+S CI + D +C +S DEI C P +I +C + S C + C + + ++ + +NC Q ++C+ + CI L+ CDG DC DG DE C+ + K P C + S C+N C+G+ DC G DE+ C+IF
Sbjct: 1468 YSDCITTCTPSQFRCDDGKCIESSKICDKVTDCSNSEDEKNCNVCDLQQFQCISNGACIPMTKKCDGTQNCEDNS--DEI----SCGCLPHQI--VC-NISRTCIEEKKICDGNYDCGVNDKS-----DEENCKKCQMTE-YKCNNSQICILLNNKCDGQDDCGDG--SDEHGCECICKEPEMILCETERTSTTEERNCQCINGTKQCDGVYDCWNGFDEKNCEIF 1676 The following BLAST results are available for this feature:
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of G-protein coupled receptor GRL101 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30018824 ID=SMED30018824|Name=G-protein coupled receptor GRL101|organism=Schmidtea mediterranea sexual|type=transcript|length=5846bpback to top protein sequence of SMED30018824-orf-1 >SMED30018824-orf-1 ID=SMED30018824-orf-1|Name=SMED30018824-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1088bp ITLFLLNFFLSSKSIFGLAINICNTHQVAMDNTKYLIIDSPYNTTSKQYYback to top protein sequence of SMED30018824-orf-2 >SMED30018824-orf-2 ID=SMED30018824-orf-2|Name=SMED30018824-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=791bp MNEFQQFLPCNRNDNQFISWNRYCDFIEDCKNGTDEKLCSYSRCAELTEWback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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