SMED30018715

Overview
NameSMED30018715
Smed IDSMED30018715
Length (bp)3182
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018715 (SMED30018715) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018715 (SMED30018715) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018715 (SMED30018715) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018715 (SMED30018715) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018715

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30018715h1SMcG0022558 SmedASXL_012557SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30018715h1SMcG0022558 SmedASXL_012557SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30018715h1SMcG0022558 Contig52113newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018715h1SMcG0022558 Contig52113uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018715h1SMcG0022558 Contig55622uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018715h1SMcG0022558 Contig55622newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30018715h1SMcG0022558 dd_Smed_v4_12596_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30018715h1SMcG0022558 dd_Smed_v4_12596_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30018715h1SMcG0022558 dd_Smed_v4_12596_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018715 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000144:198754..209533 -15681
Homology
BLAST of SMED30018715 vs. Planmine SMEST
Match: SMESG000016285.1 (SMESG000016285.1)

HSP 1 Score: 1845.09 bits (4778), Expect = 0.000e+0
Identity = 939/946 (99.26%), Postives = 942/946 (99.58%), Query Frame = 3
Query:   75 LMFYATHSKRLRKTVSNTKNQNTFRLIDNSKGITLPKFVILPNFKKINESYEIQYKSFRXXXXXXXXXXWILSVYVDQNFFYNNQSNEPLHALCYLEFSIENISNEIGQNASCKVIVRINNFTATANCSLKIGRPPSTCSTHFELGDIILSKSPTSWASVSAELXXXXXXXXXXXINRPGFLFNSFKPVNWTVVQSDEKFGKFGLGLPITSVRTGSRFKIPFSITKIDRLNSAKILMEYNDCFSFNGLENLNSSRWEIENTFYQMDSRQTGFIRVTSLDKNYRVDEELYKERKWKEIGLLELIVNENPSKDACWIRAGISKIEVAQGSTKKYFDKNNFQGFQKAISVSMDLINLIMIVFPEPRTAPVKLYSDLDYSVAPISSHVYSYLLHKSGDFYFPQLIKNLTCYSKEFSRGISKNQCVPTRFTDSWVLPVEIEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKVYRNKSCVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNRGSILVDVKSNQRTVISLVDRRNNLLADTEISVFSDHWASVVRFYGRCLSQLDLHESQSHPNGEGKFYNYNLKMRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDGMEFRNGDLVNFRQRMNIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGTPRPIVTTETITESPKTKKSSETFIEPRNSFSIAVYCIVGIVAMAVVILLLNCLIGLNVRRNSTAHMQRRLSKSGNFDRQNNIKTDYSSSQKPIQDHYXXXXXXXXXXXXXXXXXXDWAVSSESGIGLERSSLGSQQDQLGLSTFRISMANGSTHPSDSSSYIHRTSPFNDIILESNVPMYSVDSAPQPTDSQTCSNRPFWPRINSPYRGSNLDNYQDFRSPHDSTLSSESRKKLDIV 2912
            ++  ATHSKRLRKTVSNTKNQNTFRLIDNSKGITLPKFVILPNFKKINESYEIQYKSFRKSTTIISSSKWILSVYVDQNFFYNNQSNEPLHALCYLEFSIENISNEIGQNASCKVIVRINNFTATANCSLKIGRPPSTCSTHFELGDIILSKSPTSWASVSAELSFPSSPSVTSSINRPGFLFNSFKPVNWTVVQSDEKFGKF LGLPITSVRTGSRFKIPFSITKIDRLNSAKILMEYNDCFSFNGLENLNSSRWEIENTFYQMDSRQTGFIRVTSLDKNYRVDEELYKERKWKEIGLLELIVNENPSKDACWIRAGISKIEVAQGSTKKYFDKNNFQGFQKAISVSMDLINLIMIVFPEPRTAPVKLYSDLDYSVAPISSHVYSYLLHKSGDFYFPQLIKNLTCYSKEFSRGISKNQCVPTRFTDSWVLPVEIEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKVYRNKSCVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNRGSILVDVKSNQRTVISLVDRRNNLLADTEISVFSDHWASVVRFYGRCLSQLDLHESQSHPNGEGKFYNYNLKMRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDGMEFRNGDLVNFR R+NIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGTPRPIVTTETITESPKTKKSSETFIEPRNSFSIAVYCIVGIVAMAVVILLLNCLIGLNVRRNSTAHMQRRLSKSGNFDRQNNIKTDYSSSQKPIQDHYPPLLPPPGPIVPPGKPIVDWAVSSESGIGLERSSLGSQQDQLGLSTFRISMANGSTHPSDSSSYIHRTSPFNDIILESNVPMYSVDSAPQPTDSQTCSNRPFWPRINSPYRGSNLDNYQDFRSPHDSTLSSESRKKLDIV
Sbjct:    1 MILDATHSKRLRKTVSNTKNQNTFRLIDNSKGITLPKFVILPNFKKINESYEIQYKSFRKSTTIISSSKWILSVYVDQNFFYNNQSNEPLHALCYLEFSIENISNEIGQNASCKVIVRINNFTATANCSLKIGRPPSTCSTHFELGDIILSKSPTSWASVSAELSFPSSPSVTSSINRPGFLFNSFKPVNWTVVQSDEKFGKFSLGLPITSVRTGSRFKIPFSITKIDRLNSAKILMEYNDCFSFNGLENLNSSRWEIENTFYQMDSRQTGFIRVTSLDKNYRVDEELYKERKWKEIGLLELIVNENPSKDACWIRAGISKIEVAQGSTKKYFDKNNFQGFQKAISVSMDLINLIMIVFPEPRTAPVKLYSDLDYSVAPISSHVYSYLLHKSGDFYFPQLIKNLTCYSKEFSRGISKNQCVPTRFTDSWVLPVEIEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKVYRNKSCVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNRGSILVDVKSNQRTVISLVDRRNNLLADTEISVFSDHWASVVRFYGRCLSQLDLHESQSHPNGEGKFYNYNLKMRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDGMEFRNGDLVNFRLRINIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGTPRPIVTTETITESPKTKKSSETFIEPRNSFSIAVYCIVGIVAMAVVILLLNCLIGLNVRRNSTAHMQRRLSKSGNFDRQNNIKTDYSSSQKPIQDHYPPLLPPPGPIVPPGKPIVDWAVSSESGIGLERSSLGSQQDQLGLSTFRISMANGSTHPSDSSSYIHRTSPFNDIILESNVPMYSVDSAPQPTDSQTCSNRPFWPRINSPYRGSNLDNYQDFRSPHDSTLSSESRKKLDIV 946          
BLAST of SMED30018715 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 113.235 bits (282), Expect = 4.380e-25
Identity = 128/568 (22.54%), Postives = 251/568 (44.19%), Query Frame = 3
Query:  813 GLENLNSSRWEIENTFYQMDSRQTGFIRVTSLDKNYRVDEELYKERKWKEIGLLELIVNENPSKDACWIRAGISKIEVAQGSTK---------KYFDKNNFQGFQKAISVSMDLINLIMI------VFPEPRTAPVKLYSDLDYSVAPISSHVYSYLLHKSGDFYFPQLIKNLTCYSKEFSRGISKNQCVPTRFTDSWVLPVEIEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKV------YRNKSCVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNRGSILVDVKSNQRTV----ISLVDRRNNLLADTEISVFSDHWASVVRFYGRCLSQLDLHESQS--HPNGEGKFYNYNLK--MRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDGMEFRNGDLVN----------FRQRMNIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGT-PRPIVTTE-TITESPKTKKSSET----FIEPRNSFSIAVYCIVGIVAMAVVILLLNCLIGLNVRR 2381
            G+  L SS+W +       D     FI    + ++Y +  +      W    ++ L + +  SK   +    +  + +A  + K         +YF  + F    + IS+  D I L  I       F +   + +    D    + P+S       + KS D YF       T +  +   G  + + +P +F     L +E++ W+TV+F   N+W  + WKL ++   +N I+ W+V       R   CV ++Q + LK+  R+   N+ G + + G+T  +  P++D++ +   +S+  +  S+++S+       ++VD +S+   V    +S+  +R  LLA   + +  D    +      C+S LDL + +S  + N       Y L+   RK+     C L++ V  +D+SE+ L+++    YR  +   ++ +   +           +  ++NI LY S  C  +  + TG +       I+     DIP T    +++TE T T +P T  +++      I+  +S    +Y ++ +V++  +I+L+NCL+  N R+
Sbjct:  302 GMNYLKSSQWWV-------DRGLLLFIMYGRITESYMLSRKNDLRTAWASCQIINLKL-QGESKSVAYTSKILQTVHIAINTDKFIIIPILKTRYF-FSIFNDINRPISLEDDFITLYSIHDVIDGSFVDVTNSAICNSIDKSSIICPLS-------MMKSSDQYFH------TKFDNKSDNG--RTRILPNKFD----LILEMKTWKTVLFAPINLWVAQDWKLDINVKVINKINGWRVPVRSSVTREIICVTMFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDE-KFSELIVDPQSDVNDVEVRLLSVDSKR--LLAIERVKIRLDK-VIIANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKM-----CYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQLNISLYESGSCRFSKLIFTGLVR------INIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRK 826          
BLAST of SMED30018715 vs. Planmine SMEST
Match: SMESG000068172.1 (SMESG000068172.1)

HSP 1 Score: 92.4337 bits (228), Expect = 9.666e-19
Identity = 96/362 (26.52%), Postives = 164/362 (45.30%), Query Frame = 3
Query: 1347 FTDSWVLPVE-IEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKVYR--NKSCVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAK--PWVDVSKETDISSLITTKPSEVVSFSRN-NRGSILVDVK-SNQRTVISLVDRRNNL-LADTEISVFSDHWASVVRFYGRCLSQLDLHE---SQSHPNGEGKFYNYNLKMRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDG-------MEFRNGDLVNFRQ--RMNIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGTPRPIVTTETITESPKTKKSS---ETFIEPRNSFSIAVYCIVGIVAMAVVILLLNCLI 2363
            + + W + V+ I++  T++  +  VW+PESWKL LSD  L PI +W VY+  ++ CV  Y+ A LKI  RL      G + Y+G    D    P++DV+  T ++  I   P ++ S  ++ N+   L+  K +++ + I L    +   L   +IS     + SVV     C+  L L +   S+   N    + +  + +   +  + C L + V F+D S   L  +    Y+L +          +  +  LV  R   R+N+ LY    C+    + +  I    +W+    ++  +P T +P+ +T   T S    KSS   E  I   N   I++Y   G VA   +  +L  LI
Sbjct:  445 YLNPWSIQVQFIDNLHTLISPV-RVWRPESWKLMLSDEILRPIKNWAVYQPDSEECVPRYKVARLKIMCRLFLIEETGEKRYIGTEDDDTNQIPYIDVTANTKLNQ-IKFYPEDIFSIEKSLNQEYFLITAKQADKSSSIKLFSEYSQTPLTSKQISA-DQQYISVVDLIVNCIDALSLVQQKRSKKSLNSPSYYKSITVSLTSKSPSKPCYLIVFVKFSDGSIERLSLINITQYQLSLTNPDSDMFIYKITDNQLVTVRHMPRVNMSLYEDNSCSPPRLIFSKKI----EWVTVEPSNNIVPNT-QPLSSTLYTTISKHKTKSSLINEVAISSINLVHISIYICTGFVACIFIGCVLFKLI 798          
BLAST of SMED30018715 vs. Planmine SMEST
Match: SMESG000014253.1 (SMESG000014253.1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.802e-15
Identity = 73/314 (23.25%), Postives = 151/314 (48.09%), Query Frame = 3
Query: 1512 LYQSAVLKIATRLAYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNRGSILVDVKSNQRTV----ISLVDRRNNLLADTEISVFSDHWASVVRFYGRCLSQLDLHESQS--HPNGEGKFYNYNLK--MRKLALGEKCRLYLGVVFNDNSEMLLEDVAPEDYRLEVDGMEFRNGDLVN----------FRQRMNIGLYGSPQCALASAVRTGTIDLSSDWIISRDAHIDIPGT-PRPIVTTE-TITESPKTKKSSET----FIEPRNSFSIAVYCIVGIVAMAVVILLLNCLIGLNVRR 2381
            ++Q + LK+  R+   N+ G + + G+T  +  P++D++ +   +S+  +  S+++S+       ++VD +S+   V    +S+  +R  LLA   + +  D    +      C+S LDL + +S  + N       Y L+   RK+     C L++ V  +D+SE+ L+++    YR  +   ++ +   +           +  ++NI LY S  C  +  + TG +       I+     DIP T    +++TE T T +P T  +++      I+  +S    +Y ++ +V++  +I+L+NCL+  N R+
Sbjct:    1 MFQRSRLKVLVRMVNRNTLGEKEFYGSTSSNGSPFLDITSQIPKNSIEFSPSSKIISYDE-KFSELIVDPQSDVNDVEVRLLSVDSKR--LLAIERVKIRLDKVI-IANIKAFCVSSLDLFQDKSDIYSNYRRLHVEYILQNDTRKM-----CYLFIVVKLSDSSEIRLDEIPSNYYRAFITQYDYSSKHYIQVPFVVSRYILYSNQLNISLYESGSCRFSKLIFTGLVR------INIQHSEDIPTTISSTMISTEITSTINPSTVNTNQIQNRELIKNLSSLYYGLYALIALVSLVALIVLVNCLVKFNYRK 299          
BLAST of SMED30018715 vs. Planmine SMEST
Match: SMESG000036221.1 (SMESG000036221.1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.693e-11
Identity = 108/501 (21.56%), Postives = 204/501 (40.72%), Query Frame = 3
Query:  639 VNWTVVQSDEKFGKFGLGLPITSVRTGSRFKIPFSITKIDRLNSAKILMEYNDCFSFNGLENLNSSRWEIENTFYQMDSRQTGFI-RVTSLDKNYRVDEELYKERKWKEIGLLELIV-------NENPSKDACWIRAGISKIEVAQGSTKKYFDKNNFQGFQKAISVSMDLINLIMIVFPEPRTAPVKLYSDLDYSVAPIS---SHVYSYLLHKSGDFYFPQLIKNLTC-----------YSKEFSRGISKNQCVPTRFTDSWVLPVEIEHWRTVVFNIFNVWKPESWKLYLSDTELNPISDWKVYRNKS--CVFLYQSAVLKIATRLAYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNRGSILVDVKSNQRTVISLVDRRNNLLADTEISVFSDHWASV----VRFYGRCLSQLDLHESQSHPNGEGKFYNYN-LKMRKLALGEK--------CRLYLGVVFNDNSEMLLEDVAPEDYRLEV 2030
            V W ++ S + FG+  +GLP+ S+  GS+   P  I      NS  I   +      + + + +S R+             TG + ++T   K   ++E    E    +  ++EL+V       N  P K   WI   I +    +  +KK          +K IS+ +++ +    +FP  + +    +S LD  + PI    + +     H  GD  F  + +   C           + ++ S  ISK      R  D W + + +  W+  V     +W+ + W+L  +D  LN IS W  Y+N +  C  ++Q    K+  RL        R+Y G +  D  P +D++              + +  S NN     VD++++   + S+       +  +EIS+F +H + +    ++   + +S +D+  S  H     +  N N L   K+ + ++        C   + + FNDN +  L D    +++L++
Sbjct:  209 VKWKIIDSTDSFGELLIGLPLDSMEPGSKSIFPLKINSTAVYNSLAIQCSHGRSLKLSSI-SFDSVRF-------------TGTLQKITDTKKEILIEESNSLENSGGQ-RIVELLVYLRIHHLNSIPKKSDNWISCKIIQASFDKNKSKK---SRKTTEKEKKISIQVNIGHF--YIFPILKRS--IYFSILDTVMGPIEMGKNDLKILSAHNIGDESFSDITQTTECQPVSPSVLKCPFVRKTSNEISKKII---RKPD-WNVVLSLRSWKKTVTVPIELWQADDWRLTTTDRTLNKISGWN-YKNSNYQCEPVFQVTRCKLLARLMKDTFDSKRSYYGQSELDKIPLLDMTPYLR---------KQNIKLSTNNFN---VDLRNDFMLLQSV------QIGSSEISIFDNHSSKIAFDFIQADAQEVSIVDMEVSCIHDVHINQTINSNDLPFHKILVSQRLSEDSTQACYFQILLTFNDNKKSNLLDYKFTNFQLKL 664          
The following BLAST results are available for this feature:
BLAST of SMED30018715 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018715 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000016285.10.000e+099.26SMESG000016285.1[more]
SMESG000014253.14.380e-2522.54SMESG000014253.1[more]
SMESG000068172.19.666e-1926.52SMESG000068172.1[more]
SMESG000014253.15.802e-1523.25SMESG000014253.1[more]
SMESG000036221.13.693e-1121.56SMESG000036221.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018715 ID=SMED30018715|Name=SMED30018715|organism=Schmidtea mediterranea sexual|type=transcript|length=3182bp
TTTTGATAAGAATTTGAAATTTTATTTCTTGCAGATAAAAAAAGAAAAAT
ATGAAAATAATTCCGTTGATAATCTTAATGTTTTATGCTACACATTCAAA
GAGGCTAAGAAAAACAGTTTCTAATACTAAAAATCAAAATACATTTCGTT
TAATTGATAATTCTAAAGGAATAACATTACCGAAATTCGTAATTTTGCCA
AACTTTAAAAAAATCAATGAATCCTATGAAATCCAATATAAATCATTCAG
AAAAAGTACAACAATTATATCTTCTTCCAAGTGGATTTTATCTGTTTACG
TTGACCAGAACTTTTTCTACAACAATCAATCGAATGAGCCTTTGCACGCC
CTGTGTTACCTGGAATTTTCTATCGAAAATATCAGCAATGAAATCGGCCA
AAATGCCAGCTGCAAGGTGATCGTGCGCATCAATAATTTCACCGCTACTG
CGAATTGTTCATTAAAAATTGGAAGACCACCAAGCACGTGCAGCACCCAT
TTCGAGTTGGGTGATATAATCCTTTCAAAGTCCCCAACAAGCTGGGCCTC
AGTTTCAGCAGAATTATCGTTCCCCTCGTCCCCATCTGTTACAAGCTCCA
TCAATCGACCGGGGTTTCTATTCAACAGCTTCAAACCGGTCAATTGGACA
GTCGTCCAGTCAGATGAGAAATTCGGGAAATTTGGTCTCGGTCTGCCGAT
CACTTCAGTCAGAACCGGATCGAGATTCAAAATTCCGTTTTCAATTACTA
AAATTGACAGATTGAATTCCGCAAAAATCCTAATGGAATACAATGATTGT
TTCTCGTTCAACGGCCTGGAGAATCTCAACAGTTCCCGTTGGGAAATCGA
AAATACCTTCTATCAAATGGACAGCAGACAAACCGGATTTATTCGAGTGA
CTTCGCTGGATAAAAATTATCGAGTTGACGAGGAGTTGTACAAAGAGAGA
AAATGGAAAGAGATCGGGCTTTTGGAGTTAATTGTAAACGAAAATCCTTC
GAAAGATGCTTGCTGGATTCGTGCGGGAATATCAAAGATAGAAGTTGCTC
AAGGATCAACAAAAAAATACTTTGATAAAAATAATTTTCAAGGCTTCCAA
AAAGCGATTTCAGTGTCAATGGATTTGATAAACCTAATCATGATCGTATT
TCCTGAGCCCCGGACGGCCCCAGTGAAATTGTACTCAGACTTGGACTACT
CCGTTGCACCGATTTCCAGTCACGTTTACTCATATCTCTTGCATAAGTCC
GGAGATTTCTATTTCCCTCAACTTATCAAAAATTTGACTTGCTATTCCAA
GGAATTTTCCAGAGGGATTTCCAAGAATCAGTGCGTGCCAACTCGATTCA
CTGATTCCTGGGTGTTACCTGTGGAAATCGAGCATTGGAGGACTGTCGTT
TTTAATATCTTTAATGTCTGGAAGCCTGAATCGTGGAAGCTTTATCTGTC
GGATACCGAGTTGAATCCGATTAGCGACTGGAAGGTTTACCGAAACAAAA
GTTGTGTCTTTCTATACCAGTCTGCAGTGCTGAAAATTGCTACAAGGTTA
GCCTACTTCAATTCCTTTGGCATAAGGACGTACTTAGGAGCAACTTTCAA
AGACGCAAAGCCATGGGTGGATGTTAGCAAGGAAACCGACATATCCTCAC
TCATAACCACCAAGCCATCAGAAGTTGTAAGCTTCTCCAGAAACAACCGA
GGCTCCATTCTAGTTGATGTGAAGTCCAATCAACGAACTGTTATAAGTCT
GGTGGACAGGAGAAATAATTTGCTCGCCGATACTGAAATATCGGTCTTTA
GTGATCACTGGGCCTCGGTTGTGAGGTTTTATGGCCGCTGCCTTTCACAG
CTCGACCTTCACGAATCCCAATCTCATCCAAATGGGGAAGGAAAGTTCTA
CAATTACAATCTGAAAATGAGAAAGTTGGCATTGGGAGAAAAGTGTCGTT
TATATCTAGGAGTTGTGTTCAATGATAATTCAGAAATGCTGCTGGAGGAC
GTTGCCCCGGAGGATTACCGACTCGAGGTTGATGGAATGGAATTCAGGAA
TGGGGATCTTGTAAACTTCCGACAGAGAATGAATATCGGTTTGTATGGCT
CACCTCAGTGTGCGTTGGCAAGCGCCGTGAGAACTGGCACAATTGATCTG
AGCAGCGATTGGATAATCAGCAGAGATGCGCACATTGACATTCCCGGAAC
ACCTAGACCAATTGTAACCACGGAAACGATAACTGAAAGCCCGAAAACCA
AAAAATCATCTGAGACATTCATTGAACCGAGAAACTCATTTTCGATAGCC
GTTTATTGCATAGTCGGCATTGTTGCCATGGCAGTGGTGATATTGTTATT
AAATTGTTTGATCGGATTAAACGTTCGGAGAAATTCAACTGCTCATATGC
AAAGGAGACTCAGCAAATCAGGGAATTTCGACAGGCAAAATAATATCAAA
ACCGATTATTCTAGTTCCCAGAAACCCATTCAAGACCATTATCCTCCACT
GTTACCTCCTCCTGGGCCGATTGTACCTCCTGGTAAACCGATAGTTGATT
GGGCAGTCTCCTCGGAGTCTGGCATAGGATTGGAACGTTCGTCACTTGGA
TCTCAACAAGACCAGCTTGGATTATCCACTTTCAGAATATCTATGGCAAA
TGGATCAACGCATCCATCCGACTCATCGTCTTATATCCATCGGACCTCTC
CATTCAATGACATAATTCTAGAGTCAAATGTTCCTATGTACAGTGTGGAT
TCTGCTCCACAACCGACCGACTCACAAACATGCAGCAACCGGCCGTTCTG
GCCCAGAATCAACTCGCCTTATCGAGGATCCAATCTAGACAACTACCAAG
ATTTCAGGAGCCCCCATGACAGCACGCTCTCATCAGAAAGTAGAAAGAAA
TTAGACATCGTGTGATTGTCAATTGGGAGGAGTGGAGGAGGAGCAGCAGC
AGCAGGAAACCCTCTATATTTGTACAAAGATTTATCCTATTTCGTCAATG
CTCACATTGCCAGCACTCGGCGTATTGCGTACCGCCAGCACTTTCACGCA
TCAGAAACCACCGATGTGAGTGCTTATCGATTGTTATCGACAAGTGCAAT
CATTGCGCAATATTTATCAAATTCAAATTAGCCGGATCCGGGCAAATTAT
CAGATGCATTTTTGATTTTATAATACAGCTCG
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protein sequence of SMED30018715-orf-1

>SMED30018715-orf-1 ID=SMED30018715-orf-1|Name=SMED30018715-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=955bp
MKIIPLIILMFYATHSKRLRKTVSNTKNQNTFRLIDNSKGITLPKFVILP
NFKKINESYEIQYKSFRKSTTIISSSKWILSVYVDQNFFYNNQSNEPLHA
LCYLEFSIENISNEIGQNASCKVIVRINNFTATANCSLKIGRPPSTCSTH
FELGDIILSKSPTSWASVSAELSFPSSPSVTSSINRPGFLFNSFKPVNWT
VVQSDEKFGKFGLGLPITSVRTGSRFKIPFSITKIDRLNSAKILMEYNDC
FSFNGLENLNSSRWEIENTFYQMDSRQTGFIRVTSLDKNYRVDEELYKER
KWKEIGLLELIVNENPSKDACWIRAGISKIEVAQGSTKKYFDKNNFQGFQ
KAISVSMDLINLIMIVFPEPRTAPVKLYSDLDYSVAPISSHVYSYLLHKS
GDFYFPQLIKNLTCYSKEFSRGISKNQCVPTRFTDSWVLPVEIEHWRTVV
FNIFNVWKPESWKLYLSDTELNPISDWKVYRNKSCVFLYQSAVLKIATRL
AYFNSFGIRTYLGATFKDAKPWVDVSKETDISSLITTKPSEVVSFSRNNR
GSILVDVKSNQRTVISLVDRRNNLLADTEISVFSDHWASVVRFYGRCLSQ
LDLHESQSHPNGEGKFYNYNLKMRKLALGEKCRLYLGVVFNDNSEMLLED
VAPEDYRLEVDGMEFRNGDLVNFRQRMNIGLYGSPQCALASAVRTGTIDL
SSDWIISRDAHIDIPGTPRPIVTTETITESPKTKKSSETFIEPRNSFSIA
VYCIVGIVAMAVVILLLNCLIGLNVRRNSTAHMQRRLSKSGNFDRQNNIK
TDYSSSQKPIQDHYPPLLPPPGPIVPPGKPIVDWAVSSESGIGLERSSLG
SQQDQLGLSTFRISMANGSTHPSDSSSYIHRTSPFNDIILESNVPMYSVD
SAPQPTDSQTCSNRPFWPRINSPYRGSNLDNYQDFRSPHDSTLSSESRKK
LDIV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR031437TMEM132_M
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031437Transmembrane protein family 132, middle domainPFAMPF16070TMEM132coord: 454..662
e-value: 8.6E-11
score: 41.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 931..954
NoneNo IPR availableTMHMMTMhelixcoord: 749..771