SMED30018624

Overview
NameSMED30018624
Smed IDSMED30018624
Length (bp)2981
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018624 (SMED30018624) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018624 (SMED30018624) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018624 (SMED30018624) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018624 (SMED30018624) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018624

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30018624h1SMcG0015335 SmedASXL_002714SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30018624h1SMcG0015335 SmedASXL_002714SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30018624h1SMcG0015335 SmedASXL_002882SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30018624h1SMcG0015335 dd_Smed_v4_29437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30018624h1SMcG0015335 dd_Smed_v4_29437_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30018624 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 154.451 bits (389), Expect = 3.114e-37
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  359 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 764          

HSP 2 Score: 142.51 bits (358), Expect = 1.433e-33
Identity = 96/418 (22.97%), Postives = 179/418 (42.82%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  205 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 600          

HSP 3 Score: 129.798 bits (325), Expect = 9.826e-30
Identity = 103/448 (22.99%), Postives = 188/448 (41.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+  G  L     +G SP H+A +  H++ +K L+ + + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     +I+ K+   P+H+     +  ++ L L +  +  +S
Sbjct:  225 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 670          

HSP 4 Score: 126.716 bits (317), Expect = 1.008e-28
Identity = 114/475 (24.00%), Postives = 203/475 (42.74%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L      D +
Sbjct:   45 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAA 505          

HSP 5 Score: 122.865 bits (307), Expect = 1.477e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:   83 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 569          

HSP 6 Score: 102.449 bits (254), Expect = 2.955e-21
Identity = 58/238 (24.37%), Postives = 107/238 (44.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + ++    G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  555 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 7 Score: 102.064 bits (253), Expect = 3.414e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  490 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 760          

HSP 8 Score: 95.5153 bits (236), Expect = 3.517e-19
Identity = 84/406 (20.69%), Postives = 152/406 (37.44%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI----GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + +      + P+  A + G   +V LLLD   N + +       ++      K    D    H      H K   +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  444 ETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 9 Score: 88.9669 bits (219), Expect = 3.963e-17
Identity = 73/381 (19.16%), Postives = 149/381 (39.11%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS                                 ++++   K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM     +   +   L      D
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGS---------------------------------SVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 338          
BLAST of SMED30018624 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 154.066 bits (388), Expect = 3.535e-37
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  395 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 800          

HSP 2 Score: 142.51 bits (358), Expect = 1.210e-33
Identity = 96/418 (22.97%), Postives = 179/418 (42.82%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  241 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 636          

HSP 3 Score: 130.183 bits (326), Expect = 8.633e-30
Identity = 103/448 (22.99%), Postives = 188/448 (41.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+  G  L     +G SP H+A +  H++ +K L+ + + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     +I+ K+   P+H+     +  ++ L L +  +  +S
Sbjct:  261 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 706          

HSP 4 Score: 126.716 bits (317), Expect = 8.425e-29
Identity = 113/466 (24.25%), Postives = 201/466 (43.13%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L
Sbjct:   81 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL 532          

HSP 5 Score: 122.865 bits (307), Expect = 1.652e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:  119 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 605          

HSP 6 Score: 102.064 bits (253), Expect = 3.473e-21
Identity = 58/238 (24.37%), Postives = 107/238 (44.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + ++    G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  591 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 828          

HSP 7 Score: 101.679 bits (252), Expect = 5.005e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  526 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 796          

HSP 8 Score: 95.1301 bits (235), Expect = 4.745e-19
Identity = 84/406 (20.69%), Postives = 152/406 (37.44%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI----GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + +      + P+  A + G   +V LLLD   N + +       ++      K    D    H      H K   +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  480 ETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 828          

HSP 9 Score: 88.9669 bits (219), Expect = 3.627e-17
Identity = 73/381 (19.16%), Postives = 149/381 (39.11%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS                                 ++++   K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM     +   +   L      D
Sbjct:   53 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGS---------------------------------SVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 374          
BLAST of SMED30018624 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 154.066 bits (388), Expect = 3.738e-37
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  359 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 764          

HSP 2 Score: 142.124 bits (357), Expect = 1.543e-33
Identity = 96/418 (22.97%), Postives = 179/418 (42.82%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  205 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 600          

HSP 3 Score: 129.798 bits (325), Expect = 1.098e-29
Identity = 103/448 (22.99%), Postives = 188/448 (41.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+  G  L     +G SP H+A +  H++ +K L+ + + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     +I+ K+   P+H+     +  ++ L L +  +  +S
Sbjct:  225 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 670          

HSP 4 Score: 126.331 bits (316), Expect = 1.127e-28
Identity = 114/475 (24.00%), Postives = 203/475 (42.74%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L      D +
Sbjct:   45 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAA 505          

HSP 5 Score: 122.479 bits (306), Expect = 1.725e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:   83 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 569          

HSP 6 Score: 102.449 bits (254), Expect = 3.054e-21
Identity = 58/238 (24.37%), Postives = 107/238 (44.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + ++    G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  555 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 7 Score: 102.064 bits (253), Expect = 3.619e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  490 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 760          

HSP 8 Score: 95.1301 bits (235), Expect = 4.350e-19
Identity = 84/406 (20.69%), Postives = 152/406 (37.44%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI----GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + +      + P+  A + G   +V LLLD   N + +       ++      K    D    H      H K   +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  444 ETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 9 Score: 88.5817 bits (218), Expect = 4.397e-17
Identity = 73/381 (19.16%), Postives = 149/381 (39.11%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS                                 ++++   K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM     +   +   L      D
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGS---------------------------------SVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 338          
BLAST of SMED30018624 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 154.066 bits (388), Expect = 3.738e-37
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  359 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 764          

HSP 2 Score: 142.124 bits (357), Expect = 1.543e-33
Identity = 96/418 (22.97%), Postives = 179/418 (42.82%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  205 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 600          

HSP 3 Score: 129.798 bits (325), Expect = 1.098e-29
Identity = 103/448 (22.99%), Postives = 188/448 (41.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+  G  L     +G SP H+A +  H++ +K L+ + + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     +I+ K+   P+H+     +  ++ L L +  +  +S
Sbjct:  225 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 670          

HSP 4 Score: 126.331 bits (316), Expect = 1.127e-28
Identity = 114/475 (24.00%), Postives = 203/475 (42.74%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L      D +
Sbjct:   45 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAA 505          

HSP 5 Score: 122.479 bits (306), Expect = 1.725e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:   83 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 569          

HSP 6 Score: 102.449 bits (254), Expect = 3.054e-21
Identity = 58/238 (24.37%), Postives = 107/238 (44.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + ++    G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  555 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 7 Score: 102.064 bits (253), Expect = 3.619e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  490 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 760          

HSP 8 Score: 95.1301 bits (235), Expect = 4.350e-19
Identity = 84/406 (20.69%), Postives = 152/406 (37.44%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI----GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + +      + P+  A + G   +V LLLD   N + +       ++      K    D    H      H K   +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  444 ETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 9 Score: 88.5817 bits (218), Expect = 4.397e-17
Identity = 73/381 (19.16%), Postives = 149/381 (39.11%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS                                 ++++   K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM     +   +   L      D
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGS---------------------------------SVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 338          
BLAST of SMED30018624 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 153.68 bits (387), Expect = 3.748e-37
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  359 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 764          

HSP 2 Score: 142.51 bits (358), Expect = 1.290e-33
Identity = 96/418 (22.97%), Postives = 179/418 (42.82%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  205 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 600          

HSP 3 Score: 130.183 bits (326), Expect = 7.670e-30
Identity = 103/448 (22.99%), Postives = 188/448 (41.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+  G  L     +G SP H+A +  H++ +K L+ + + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     +I+ K+   P+H+     +  ++ L L +  +  +S
Sbjct:  225 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 670          

HSP 4 Score: 126.716 bits (317), Expect = 8.255e-29
Identity = 113/466 (24.25%), Postives = 201/466 (43.13%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L
Sbjct:   45 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL 496          

HSP 5 Score: 122.479 bits (306), Expect = 1.626e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:   83 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 569          

HSP 6 Score: 102.449 bits (254), Expect = 2.826e-21
Identity = 58/238 (24.37%), Postives = 107/238 (44.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + ++    G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  555 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 7 Score: 101.679 bits (252), Expect = 4.179e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  490 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 760          

HSP 8 Score: 95.1301 bits (235), Expect = 5.103e-19
Identity = 84/409 (20.54%), Postives = 153/409 (37.41%), Query Frame = 2
Query:   83 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI----GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            +  E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + +      + P+  A + G   +V LLLD   N + +       ++      K    D    H      H K   +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  441 IRGETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 9 Score: 88.1965 bits (217), Expect = 6.101e-17
Identity = 73/381 (19.16%), Postives = 148/381 (38.85%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS +++                                    K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM     +   +   L      D
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATK---------------------------------KGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 338          
BLAST of SMED30018624 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 145.591 bits (366), Expect = 6.151e-35
Identity = 105/435 (24.14%), Postives = 186/435 (42.76%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  ++ +      N + P+H A   + + +VELL+  +  IEA  +  + P+H+A   G ++IV  L+  G +  V  + G +P HLA      D+++ LI NG+ ++A+    Q  +H A R    +I   L+    N  A       P+HIA + G E++  +L+  N D       G  P+HLA   G+L ++  L +     D      + P+  A       +  LLL+N  +  K          H+   K    I    +        +S     PL++S Q GH +I  L++    D   +    LT     + ED +    ++ +      +         H+AC  G LN+V++L++ G +D+  +  +    +H A    H + VR+LL+     +      Q P+ +     Y S++
Sbjct:  372 VRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASA-KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENG-ADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVV 804          

HSP 2 Score: 136.732 bits (343), Expect = 3.169e-32
Identity = 96/417 (23.02%), Postives = 186/417 (44.60%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHI-------GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D      LL +   N +  +++ + P+H A    +  + +LL+    ++  +    + P+H+A K+G  ++  LL+S G  +     D  +P H A   GH  ++  L+  G+ + A+       +H A + + ++ A+ L+ +   ++     Y  P+H+A   G   +  LL+ ++ D N+    G  P+H+AC    ++++E L K +   ++     + P+  A  +G  +IV  LL    N + +T+  E      L  + +  + D  ++ I          +E+++PL+++ + G+ DIV L+L A   N    T + +S                     HIA   G   V   LLD   +D       G   +H+A  + ++++VR LL+R    DI  K    P+H+
Sbjct:  205 DDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETP----LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGA-NSNATTRDNYSP-------------------LHIAAKEGQEEVAGILLDHN-ADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHV 596          

HSP 3 Score: 136.346 bits (342), Expect = 4.165e-32
Identity = 117/507 (23.08%), Postives = 215/507 (42.41%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL-RNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL--SRNCSFDLSDEYKSINSYKKSEKLEKA 1474
            +A   GDL +VL LL R  T+I   N N    +H A    +  +V  LI  +  ++A   +    +H+A   G   IV +L+ NG +++V  ++G +P ++A +  H +++K+L+ +G++  L   + F+   +                           H A + +    A  L+ N  N +  +     P+HIA  YG E++  LL+++  +VN +  +   P+H+A   G   +   L       DS     + P+  A + G   +V LL+      +         +  L  + +   +D  +  + H   ++       +PL+V+   GH+ + +L+LD     N R L            N     E ++K ++ I+   E   T  H+A   G +N+V YLL  G ++ + E + G+  +H+A   +  D+VR L+      D   +  Q P+H+        I+ L L    N +    D Y  ++   K  + E A
Sbjct:   39 RAARAGDLEKVLELL-RAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTK---NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQG-ANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVA 540          

HSP 4 Score: 112.464 bits (280), Expect = 9.425e-25
Identity = 94/457 (20.57%), Postives = 194/457 (42.45%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN----TNK---TIYMEHK----EVYHLFYSKKTK-----RIDRFKMHIGHH---------------------KEIESPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +V++LL  +   I    +N   P+H A    +V     L+ ++  ++      + P+H+A   G++ + +LL+    D +   L+G +P H+AC+   + +++ L+   + +EA        +H A     I I  +L+    N +    + + P+H+A      D+  +LI+    V+A+      P+H+A   G+  I+  L +   + ++    N  P+  A K G + +  +LLD+N +    T K    +++  K    EV  L   + T      +     +H+  H                     K   +PL+++ ++  ++I   +L    D   ++    T   H SA++        +I+  S +  +     T  H+     H+ V Q L + G ++I  +  +G   +H+AC F  +++V+FL++         +    P+H 
Sbjct:  307 QVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFT-PLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNG-AEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQ 761          

HSP 5 Score: 110.923 bits (276), Expect = 2.871e-24
Identity = 61/238 (25.63%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV+ LL  + T ++   +N   P+H A   +N  +  LL+ N    +A       P+H+A K   ++I   L+    D +     G +P HL+ + GH +I   LI NGSD+ A+ N     +H   + + + +A+ L +N   + +       P+H+AC +G  ++   L++   DV  +      P+H A   GH   + +L +     +        P+  A +LG  S+V+ L
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          
BLAST of SMED30018624 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 145.591 bits (366), Expect = 6.151e-35
Identity = 105/435 (24.14%), Postives = 186/435 (42.76%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  ++ +      N + P+H A   + + +VELL+  +  IEA  +  + P+H+A   G ++IV  L+  G +  V  + G +P HLA      D+++ LI NG+ ++A+    Q  +H A R    +I   L+    N  A       P+HIA + G E++  +L+  N D       G  P+HLA   G+L ++  L +     D      + P+  A       +  LLL+N  +  K          H+   K    I    +        +S     PL++S Q GH +I  L++    D   +    LT     + ED +    ++ +      +         H+AC  G LN+V++L++ G +D+  +  +    +H A    H + VR+LL+     +      Q P+ +     Y S++
Sbjct:  372 VRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASA-KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENG-ADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVV 804          

HSP 2 Score: 136.732 bits (343), Expect = 3.169e-32
Identity = 96/417 (23.02%), Postives = 186/417 (44.60%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHI-------GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D      LL +   N +  +++ + P+H A    +  + +LL+    ++  +    + P+H+A K+G  ++  LL+S G  +     D  +P H A   GH  ++  L+  G+ + A+       +H A + + ++ A+ L+ +   ++     Y  P+H+A   G   +  LL+ ++ D N+    G  P+H+AC    ++++E L K +   ++     + P+  A  +G  +IV  LL    N + +T+  E      L  + +  + D  ++ I          +E+++PL+++ + G+ DIV L+L A   N    T + +S                     HIA   G   V   LLD   +D       G   +H+A  + ++++VR LL+R    DI  K    P+H+
Sbjct:  205 DDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETP----LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGA-NSNATTRDNYSP-------------------LHIAAKEGQEEVAGILLDHN-ADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHV 596          

HSP 3 Score: 136.346 bits (342), Expect = 4.165e-32
Identity = 117/507 (23.08%), Postives = 215/507 (42.41%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL-RNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL--SRNCSFDLSDEYKSINSYKKSEKLEKA 1474
            +A   GDL +VL LL R  T+I   N N    +H A    +  +V  LI  +  ++A   +    +H+A   G   IV +L+ NG +++V  ++G +P ++A +  H +++K+L+ +G++  L   + F+   +                           H A + +    A  L+ N  N +  +     P+HIA  YG E++  LL+++  +VN +  +   P+H+A   G   +   L       DS     + P+  A + G   +V LL+      +         +  L  + +   +D  +  + H   ++       +PL+V+   GH+ + +L+LD     N R L            N     E ++K ++ I+   E   T  H+A   G +N+V YLL  G ++ + E + G+  +H+A   +  D+VR L+      D   +  Q P+H+        I+ L L    N +    D Y  ++   K  + E A
Sbjct:   39 RAARAGDLEKVLELL-RAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTK---NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQG-ANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVA 540          

HSP 4 Score: 112.464 bits (280), Expect = 9.425e-25
Identity = 94/457 (20.57%), Postives = 194/457 (42.45%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN----TNK---TIYMEHK----EVYHLFYSKKTK-----RIDRFKMHIGHH---------------------KEIESPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +V++LL  +   I    +N   P+H A    +V     L+ ++  ++      + P+H+A   G++ + +LL+    D +   L+G +P H+AC+   + +++ L+   + +EA        +H A     I I  +L+    N +    + + P+H+A      D+  +LI+    V+A+      P+H+A   G+  I+  L +   + ++    N  P+  A K G + +  +LLD+N +    T K    +++  K    EV  L   + T      +     +H+  H                     K   +PL+++ ++  ++I   +L    D   ++    T   H SA++        +I+  S +  +     T  H+     H+ V Q L + G ++I  +  +G   +H+AC F  +++V+FL++         +    P+H 
Sbjct:  307 QVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFT-PLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNG-AEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQ 761          

HSP 5 Score: 110.923 bits (276), Expect = 2.871e-24
Identity = 61/238 (25.63%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV+ LL  + T ++   +N   P+H A   +N  +  LL+ N    +A       P+H+A K   ++I   L+    D +     G +P HL+ + GH +I   LI NGSD+ A+ N     +H   + + + +A+ L +N   + +       P+H+AC +G  ++   L++   DV  +      P+H A   GH   + +L +     +        P+  A +LG  S+V+ L
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          
BLAST of SMED30018624 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 145.591 bits (366), Expect = 6.428e-35
Identity = 105/435 (24.14%), Postives = 186/435 (42.76%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  ++ +      N + P+H A   + + +VELL+  +  IEA  +  + P+H+A   G ++IV  L+  G +  V  + G +P HLA      D+++ LI NG+ ++A+    Q  +H A R    +I   L+    N  A       P+HIA + G E++  +L+  N D       G  P+HLA   G+L ++  L +     D      + P+  A       +  LLL+N  +  K          H+   K    I    +        +S     PL++S Q GH +I  L++    D   +    LT     + ED +    ++ +      +         H+AC  G LN+V++L++ G +D+  +  +    +H A    H + VR+LL+     +      Q P+ +     Y S++
Sbjct:  372 VRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASA-KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENG-ADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVV 804          

HSP 2 Score: 136.732 bits (343), Expect = 3.094e-32
Identity = 95/417 (22.78%), Postives = 186/417 (44.60%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHI-------GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D      LL +   N +  +++ + P+H A    +  + +LL+    ++  +    + P+H+A K+G  ++  LL+S G  +     D  +P H A   GH  ++  L+  G+ + A+       +H A + + ++ A+ L+ +   ++     Y  P+H+A   G   +  LL+ ++ D N+    G  P+H+AC    ++++E L K +   ++     + P+  A  +G  +IV  LL    N + +T+  E      L  + +  + D  ++ I          +E+++PL+++ + G+ DIV L+L A          N +++  D                 HIA   G   V   LLD   +D       G   +H+A  + ++++VR LL+R    DI  K    P+H+
Sbjct:  205 DDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETP----LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQA--------GANSNATTRDNYS------------PLHIAAKEGQEEVAGILLDHN-ADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHV 596          

HSP 3 Score: 136.346 bits (342), Expect = 3.831e-32
Identity = 117/507 (23.08%), Postives = 215/507 (42.41%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL-RNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL--SRNCSFDLSDEYKSINSYKKSEKLEKA 1474
            +A   GDL +VL LL R  T+I   N N    +H A    +  +V  LI  +  ++A   +    +H+A   G   IV +L+ NG +++V  ++G +P ++A +  H +++K+L+ +G++  L   + F+   +                           H A + +    A  L+ N  N +  +     P+HIA  YG E++  LL+++  +VN +  +   P+H+A   G   +   L       DS     + P+  A + G   +V LL+      +         +  L  + +   +D  +  + H   ++       +PL+V+   GH+ + +L+LD     N R L            N     E ++K ++ I+   E   T  H+A   G +N+V YLL  G ++ + E + G+  +H+A   +  D+VR L+      D   +  Q P+H+        I+ L L    N +    D Y  ++   K  + E A
Sbjct:   39 RAARAGDLEKVLELL-RAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTK---NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQG-ANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVA 540          

HSP 4 Score: 112.464 bits (280), Expect = 9.458e-25
Identity = 94/457 (20.57%), Postives = 194/457 (42.45%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN----TNK---TIYMEHK----EVYHLFYSKKTK-----RIDRFKMHIGHH---------------------KEIESPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +V++LL  +   I    +N   P+H A    +V     L+ ++  ++      + P+H+A   G++ + +LL+    D +   L+G +P H+AC+   + +++ L+   + +EA        +H A     I I  +L+    N +    + + P+H+A      D+  +LI+    V+A+      P+H+A   G+  I+  L +   + ++    N  P+  A K G + +  +LLD+N +    T K    +++  K    EV  L   + T      +     +H+  H                     K   +PL+++ ++  ++I   +L    D   ++    T   H SA++        +I+  S +  +     T  H+     H+ V Q L + G ++I  +  +G   +H+AC F  +++V+FL++         +    P+H 
Sbjct:  307 QVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFT-PLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNG-AEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQ 761          

HSP 5 Score: 110.923 bits (276), Expect = 2.738e-24
Identity = 61/238 (25.63%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV+ LL  + T ++   +N   P+H A   +N  +  LL+ N    +A       P+H+A K   ++I   L+    D +     G +P HL+ + GH +I   LI NGSD+ A+ N     +H   + + + +A+ L +N   + +       P+H+AC +G  ++   L++   DV  +      P+H A   GH   + +L +     +        P+  A +LG  S+V+ L
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          
BLAST of SMED30018624 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 145.591 bits (366), Expect = 6.428e-35
Identity = 105/435 (24.14%), Postives = 186/435 (42.76%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  ++ +      N + P+H A   + + +VELL+  +  IEA  +  + P+H+A   G ++IV  L+  G +  V  + G +P HLA      D+++ LI NG+ ++A+    Q  +H A R    +I   L+    N  A       P+HIA + G E++  +L+  N D       G  P+HLA   G+L ++  L +     D      + P+  A       +  LLL+N  +  K          H+   K    I    +        +S     PL++S Q GH +I  L++    D   +    LT     + ED +    ++ +      +         H+AC  G LN+V++L++ G +D+  +  +    +H A    H + VR+LL+     +      Q P+ +     Y S++
Sbjct:  372 VRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASA-KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENG-ADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVV 804          

HSP 2 Score: 136.732 bits (343), Expect = 3.094e-32
Identity = 95/417 (22.78%), Postives = 186/417 (44.60%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHI-------GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D      LL +   N +  +++ + P+H A    +  + +LL+    ++  +    + P+H+A K+G  ++  LL+S G  +     D  +P H A   GH  ++  L+  G+ + A+       +H A + + ++ A+ L+ +   ++     Y  P+H+A   G   +  LL+ ++ D N+    G  P+H+AC    ++++E L K +   ++     + P+  A  +G  +IV  LL    N + +T+  E      L  + +  + D  ++ I          +E+++PL+++ + G+ DIV L+L A          N +++  D                 HIA   G   V   LLD   +D       G   +H+A  + ++++VR LL+R    DI  K    P+H+
Sbjct:  205 DDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETP----LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQA--------GANSNATTRDNYS------------PLHIAAKEGQEEVAGILLDHN-ADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHV 596          

HSP 3 Score: 136.346 bits (342), Expect = 3.831e-32
Identity = 117/507 (23.08%), Postives = 215/507 (42.41%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL-RNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL--SRNCSFDLSDEYKSINSYKKSEKLEKA 1474
            +A   GDL +VL LL R  T+I   N N    +H A    +  +V  LI  +  ++A   +    +H+A   G   IV +L+ NG +++V  ++G +P ++A +  H +++K+L+ +G++  L   + F+   +                           H A + +    A  L+ N  N +  +     P+HIA  YG E++  LL+++  +VN +  +   P+H+A   G   +   L       DS     + P+  A + G   +V LL+      +         +  L  + +   +D  +  + H   ++       +PL+V+   GH+ + +L+LD     N R L            N     E ++K ++ I+   E   T  H+A   G +N+V YLL  G ++ + E + G+  +H+A   +  D+VR L+      D   +  Q P+H+        I+ L L    N +    D Y  ++   K  + E A
Sbjct:   39 RAARAGDLEKVLELL-RAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTK---NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQG-ANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVA 540          

HSP 4 Score: 112.464 bits (280), Expect = 9.458e-25
Identity = 94/457 (20.57%), Postives = 194/457 (42.45%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN----TNK---TIYMEHK----EVYHLFYSKKTK-----RIDRFKMHIGHH---------------------KEIESPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +V++LL  +   I    +N   P+H A    +V     L+ ++  ++      + P+H+A   G++ + +LL+    D +   L+G +P H+AC+   + +++ L+   + +EA        +H A     I I  +L+    N +    + + P+H+A      D+  +LI+    V+A+      P+H+A   G+  I+  L +   + ++    N  P+  A K G + +  +LLD+N +    T K    +++  K    EV  L   + T      +     +H+  H                     K   +PL+++ ++  ++I   +L    D   ++    T   H SA++        +I+  S +  +     T  H+     H+ V Q L + G ++I  +  +G   +H+AC F  +++V+FL++         +    P+H 
Sbjct:  307 QVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFT-PLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNG-AEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQ 761          

HSP 5 Score: 110.923 bits (276), Expect = 2.738e-24
Identity = 61/238 (25.63%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV+ LL  + T ++   +N   P+H A   +N  +  LL+ N    +A       P+H+A K   ++I   L+    D +     G +P HL+ + GH +I   LI NGSD+ A+ N     +H   + + + +A+ L +N   + +       P+H+AC +G  ++   L++   DV  +      P+H A   GH   + +L +     +        P+  A +LG  S+V+ L
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          
BLAST of SMED30018624 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 145.206 bits (365), Expect = 7.941e-35
Identity = 105/435 (24.14%), Postives = 186/435 (42.76%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  ++ +      N + P+H A   + + +VELL+  +  IEA  +  + P+H+A   G ++IV  L+  G +  V  + G +P HLA      D+++ LI NG+ ++A+    Q  +H A R    +I   L+    N  A       P+HIA + G E++  +L+  N D       G  P+HLA   G+L ++  L +     D      + P+  A       +  LLL+N  +  K          H+   K    I    +        +S     PL++S Q GH +I  L++    D   +    LT     + ED +    ++ +      +         H+AC  G LN+V++L++ G +D+  +  +    +H A    H + VR+LL+     +      Q P+ +     Y S++
Sbjct:  372 VRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASA-KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENG-ADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVV 804          

HSP 2 Score: 136.346 bits (342), Expect = 3.720e-32
Identity = 96/417 (23.02%), Postives = 186/417 (44.60%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHI-------GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D      LL +   N +  +++ + P+H A    +  + +LL+    ++  +    + P+H+A K+G  ++  LL+S G  +     D  +P H A   GH  ++  L+  G+ + A+       +H A + + ++ A+ L+ +   ++     Y  P+H+A   G   +  LL+ ++ D N+    G  P+H+AC    ++++E L K +   ++     + P+  A  +G  +IV  LL    N + +T+  E      L  + +  + D  ++ I          +E+++PL+++ + G+ DIV L+L A   N    T + +S                     HIA   G   V   LLD   +D       G   +H+A  + ++++VR LL+R    DI  K    P+H+
Sbjct:  205 DDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETP----LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGA-NSNATTRDNYSP-------------------LHIAAKEGQEEVAGILLDHN-ADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHV 596          

HSP 3 Score: 135.961 bits (341), Expect = 4.889e-32
Identity = 117/507 (23.08%), Postives = 215/507 (42.41%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL-RNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL--SRNCSFDLSDEYKSINSYKKSEKLEKA 1474
            +A   GDL +VL LL R  T+I   N N    +H A    +  +V  LI  +  ++A   +    +H+A   G   IV +L+ NG +++V  ++G +P ++A +  H +++K+L+ +G++  L   + F+   +                           H A + +    A  L+ N  N +  +     P+HIA  YG E++  LL+++  +VN +  +   P+H+A   G   +   L       DS     + P+  A + G   +V LL+      +         +  L  + +   +D  +  + H   ++       +PL+V+   GH+ + +L+LD     N R L            N     E ++K ++ I+   E   T  H+A   G +N+V YLL  G ++ + E + G+  +H+A   +  D+VR L+      D   +  Q P+H+        I+ L L    N +    D Y  ++   K  + E A
Sbjct:   39 RAARAGDLEKVLELL-RAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTK---NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQG-ANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVA 540          

HSP 4 Score: 112.079 bits (279), Expect = 1.123e-24
Identity = 94/457 (20.57%), Postives = 194/457 (42.45%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN----TNK---TIYMEHK----EVYHLFYSKKTK-----RIDRFKMHIGHH---------------------KEIESPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +V++LL  +   I    +N   P+H A    +V     L+ ++  ++      + P+H+A   G++ + +LL+    D +   L+G +P H+AC+   + +++ L+   + +EA        +H A     I I  +L+    N +    + + P+H+A      D+  +LI+    V+A+      P+H+A   G+  I+  L +   + ++    N  P+  A K G + +  +LLD+N +    T K    +++  K    EV  L   + T      +     +H+  H                     K   +PL+++ ++  ++I   +L    D   ++    T   H SA++        +I+  S +  +     T  H+     H+ V Q L + G ++I  +  +G   +H+AC F  +++V+FL++         +    P+H 
Sbjct:  307 QVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFT-PLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNG-AEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQ 761          

HSP 5 Score: 110.923 bits (276), Expect = 2.985e-24
Identity = 61/238 (25.63%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV+ LL  + T ++   +N   P+H A   +N  +  LL+ N    +A       P+H+A K   ++I   L+    D +     G +P HL+ + GH +I   LI NGSD+ A+ N     +H   + + + +A+ L +N   + +       P+H+AC +G  ++   L++   DV  +      P+H A   GH   + +L +     +        P+  A +LG  S+V+ L
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          
BLAST of SMED30018624 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303119)

HSP 1 Score: 152.91 bits (385), Expect = 2.670e-37
Identity = 108/435 (24.83%), Postives = 194/435 (44.60%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARL---RNVRNLTNNCH--------SSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  +  +      N + P+H A   + + +VELL+ +   I A  +  + P+H+A   G ++IV  L+ +     V  + G +P HLA      DII+ L+ NG+ ++AR    Q  +H A R   ++I   L+ +   ++A        +HIA + G +++ ++LI+    ++A    G  P+HL    GH+++ + L + + D D+     + P+  AC    + +  LLL+   + + T    H  + H+   K    I    +  G     ES     PL++S Q GH +I  L+++ +       +N     H        + AE + K  + ID   +   T  H+A   G  N+V++LL  G ++++     G   +H      H  IV  LL+     +      Q P+H+     Y S+L
Sbjct:  350 VRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPL-HIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNG-ANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVL 782          

HSP 2 Score: 149.443 bits (376), Expect = 3.070e-36
Identity = 100/428 (23.36%), Postives = 194/428 (45.33%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELIL------DARLRNVRNLTNNCHSSAED-----MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D ++   LL     N +  +++ + P+H A    N  I  LLI    D+       + P+H+A K+G  ++V LL+  G ++     DG +P H A   GH  ++  L+  G+ + A+       +H A +   ++ A+ L+ +   ++     Y   +H+A   G   +  LL+ +N D NA    G  P+H+AC    L+++E L +      +     + P+  A  +G  +IV  LL ++ + +  T+  E     HL        I R  +  G       +E ++PL+++ + G++DIV L+L      DA  +++    +      +D     +I+  + +D   ++  T  H+    GH+ V Q LL    +D++ +  +G   +H+AC +++  +   LL++        K    P+H+
Sbjct:  183 DDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQ-KEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHI 607          

HSP 3 Score: 124.79 bits (312), Expect = 1.374e-28
Identity = 101/472 (21.40%), Postives = 203/472 (43.01%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +A   G+L  VL  L +   +I   N N    +H A  + ++ +V  L+     +++   +    +H+A   G  ++V+LL+ +   ++V   +G +P ++A +  H  +++ L+SNG++  L   + F+   +                           H A + + ++ A  L+ N+ N +  +     P+HIA  YG ++I +LLIQ+  DVN    +   P+H+A   G   ++  L +   + ++     + P+  A + G + +V +LL+     +         +  L  + + + +D  ++ + H   ++       + L+V+   GH+ + +L+LD           N  ++A  +     L          HIAC    L VV+ LL  G S I     SG   +H+A     ++IV +LL  +   D+   R + P+H+   ++   I+ + L      D
Sbjct:   17 RAARAGNLERVLEHL-KNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTK---NGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD----------RNADANARALNGFTPL----------HIACKKNRLKVVELLLRHGAS-ISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVD 463          

HSP 4 Score: 124.405 bits (311), Expect = 1.986e-28
Identity = 105/480 (21.88%), Postives = 197/480 (41.04%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCD---------------------------IEAENDQKMR--PIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS--DEYKSINSYKKSEKLEKA 1474
            EV+ LL     ++   ++N + P++ A   ++ A+V LL+SN  +                           +E++   K+R   +H+A K  ++    LL+ N  +  V    G +P H+A  +G+ +I   LI  G+D+   A++N S   +H A ++ +  +   L+    N+EA       P+H A   G E +  +L+++   ++A+   G  P+H+A    H+     L   +   D      +  +  A   G   + +LLLD N + N                   + ++ F           +PL+++C++  L +VEL+    LR+  +++    S    +                H+A   G +N+V YLL    S  +   + G+  +H+A   +  DI+R LL      D   +  Q P+H+        I+ L L      D +  D Y +++   K  + E A
Sbjct:   91 EVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISP--LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADAN------------------ARALNGF-----------TPLHIACKKNRLKVVELL----LRHGASISATTESGLTPL----------------HVAAFMGCMNIVIYLLQHDASP-DVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVA 518          

HSP 5 Score: 120.168 bits (300), Expect = 3.466e-27
Identity = 97/430 (22.56%), Postives = 188/430 (43.72%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI--------GHHKEIESPLYVSCQRGHLDIVELIL------DARLRN-VRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +++LL  K  NIE    +   P+H A  + +  +V++L+     I A+    + P+H+A +  ++D  ++L+ +   +    +D  +  H+A   GH+ + K L+   +D  AR  N F+   +H AC+ NR+++ + L+ +  ++ A       P+H+A   G  +I   L+Q +   +     G  P+HLA       II  L +     D+       P+  A +LG   IV LLL +    + T     K++Y   +    +  D     +           K+  +PL+++ + GH+ + +L+L      DA+ +N V  L   CH + + +     L++       T        HIA     +++   LL+ G +    E  +G   +H++    H +I   L++     +   K    P+H+
Sbjct:  253 MVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTP--LHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT----KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALL--LLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYG-ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHL 673          

HSP 6 Score: 98.5969 bits (244), Expect = 1.777e-20
Identity = 60/270 (22.22%), Postives = 109/270 (40.37%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNG----------------SDLEARNNFSQYLIHNACRFNR-----------------IEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            EV  +L      ++   +  + P+H      ++ + +LL+  + D++A+    + P+H+AC Y N  +  LL+  G        +G++P H+A     +DI   L+  G                S  E     S  LI +    N                  + +A+ L  N  N++        P+H+A  +G  ++   L+Q   +V+A  S G  P+H     GH  I+  L + K + ++       P+  A KLG  S++  L
Sbjct:  516 EVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          

HSP 7 Score: 54.6842 bits (130), Expect = 4.798e-7
Identity = 27/112 (24.11%), Postives = 54/112 (48.21%), Query Frame = 2
Query:  113 CLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFL 448
            C   D + V  +L +   NI+   +  Y P+H A       +V  L+ N  +++A       P+H   + G+  IV LL+ +  + +   ++G +P H+A + G++ ++  L
Sbjct:  674 CAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          
BLAST of SMED30018624 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334061)

HSP 1 Score: 151.754 bits (382), Expect = 7.181e-37
Identity = 108/435 (24.83%), Postives = 194/435 (44.60%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARL---RNVRNLTNNCH--------SSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            + V  LL  +  +      N + P+H A   + + +VELL+ +   I A  +  + P+H+A   G ++IV  L+ +     V  + G +P HLA      DII+ L+ NG+ ++AR    Q  +H A R   ++I   L+ +   ++A        +HIA + G +++ ++LI+    ++A    G  P+HL    GH+++ + L + + D D+     + P+  AC    + +  LLL+   + + T    H  + H+   K    I    +  G     ES     PL++S Q GH +I  L+++ +       +N     H        + AE + K  + ID   +   T  H+A   G  N+V++LL  G ++++     G   +H      H  IV  LL+     +      Q P+H+     Y S+L
Sbjct:  500 VRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPL-HIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNG-ANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVL 932          

HSP 2 Score: 148.673 bits (374), Expect = 6.123e-36
Identity = 100/428 (23.36%), Postives = 194/428 (45.33%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELIL------DARLRNVRNLTNNCHSSAED-----MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            D ++   LL     N +  +++ + P+H A    N  I  LLI    D+       + P+H+A K+G  ++V LL+  G ++     DG +P H A   GH  ++  L+  G+ + A+       +H A +   ++ A+ L+ +   ++     Y   +H+A   G   +  LL+ +N D NA    G  P+H+AC    L+++E L +      +     + P+  A  +G  +IV  LL ++ + +  T+  E     HL        I R  +  G       +E ++PL+++ + G++DIV L+L      DA  +++    +      +D     +I+  + +D   ++  T  H+    GH+ V Q LL    +D++ +  +G   +H+AC +++  +   LL++        K    P+H+
Sbjct:  333 DDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQ-KEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHI 757          

HSP 3 Score: 123.635 bits (309), Expect = 3.490e-28
Identity = 105/480 (21.88%), Postives = 197/480 (41.04%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCD---------------------------IEAENDQKMR--PIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS--DEYKSINSYKKSEKLEKA 1474
            EV+ LL     ++   ++N + P++ A   ++ A+V LL+SN  +                           +E++   K+R   +H+A K  ++    LL+ N  +  V    G +P H+A  +G+ +I   LI  G+D+   A++N S   +H A ++ +  +   L+    N+EA       P+H A   G E +  +L+++   ++A+   G  P+H+A    H+     L   +   D      +  +  A   G   + +LLLD N + N                   + ++ F           +PL+++C++  L +VEL+    LR+  +++    S    +                H+A   G +N+V YLL    S  +   + G+  +H+A   +  DI+R LL      D   +  Q P+H+        I+ L L      D +  D Y +++   K  + E A
Sbjct:  241 EVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISP--LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADAN------------------ARALNGF-----------TPLHIACKKNRLKVVELL----LRHGASISATTESGLTPL----------------HVAAFMGCMNIVIYLLQHDASP-DVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVA 668          

HSP 4 Score: 123.25 bits (308), Expect = 4.176e-28
Identity = 101/472 (21.40%), Postives = 203/472 (43.01%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +A   G+L  VL  L +   +I   N N    +H A  + ++ +V  L+     +++   +    +H+A   G  ++V+LL+ +   ++V   +G +P ++A +  H  +++ L+SNG++  L   + F+   +                           H A + + ++ A  L+ N+ N +  +     P+HIA  YG ++I +LLIQ+  DVN    +   P+H+A   G   ++  L +   + ++     + P+  A + G + +V +LL+     +         +  L  + + + +D  ++ + H   ++       + L+V+   GH+ + +L+LD           N  ++A  +     L          HIAC    L VV+ LL  G S I     SG   +H+A     ++IV +LL  +   D+   R + P+H+   ++   I+ + L      D
Sbjct:  167 RAARAGNLERVLEHL-KNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTK---NGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD----------RNADANARALNGFTPL----------HIACKKNRLKVVELLLRHGAS-ISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVD 613          

HSP 5 Score: 119.398 bits (298), Expect = 7.759e-27
Identity = 97/430 (22.56%), Postives = 188/430 (43.72%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI--------GHHKEIESPLYVSCQRGHLDIVELIL------DARLRN-VRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +++LL  K  NIE    +   P+H A  + +  +V++L+     I A+    + P+H+A +  ++D  ++L+ +   +    +D  +  H+A   GH+ + K L+   +D  AR  N F+   +H AC+ NR+++ + L+ +  ++ A       P+H+A   G  +I   L+Q +   +     G  P+HLA       II  L +     D+       P+  A +LG   IV LLL +    + T     K++Y   +    +  D     +           K+  +PL+++ + GH+ + +L+L      DA+ +N V  L   CH + + +     L++       T        HIA     +++   LL+ G +    E  +G   +H++    H +I   L++     +   K    P+H+
Sbjct:  403 MVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTP--LHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATT----KDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALL--LLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYG-ALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHL 823          

HSP 6 Score: 98.9821 bits (245), Expect = 1.491e-20
Identity = 60/270 (22.22%), Postives = 109/270 (40.37%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNG----------------SDLEARNNFSQYLIHNACRFNR-----------------IEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            EV  +L      ++   +  + P+H      ++ + +LL+  + D++A+    + P+H+AC Y N  +  LL+  G        +G++P H+A     +DI   L+  G                S  E     S  LI +    N                  + +A+ L  N  N++        P+H+A  +G  ++   L+Q   +V+A  S G  P+H     GH  I+  L + K + ++       P+  A KLG  S++  L
Sbjct:  666 EVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 935          

HSP 7 Score: 55.0694 bits (131), Expect = 3.478e-7
Identity = 27/112 (24.11%), Postives = 54/112 (48.21%), Query Frame = 2
Query:  113 CLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFL 448
            C   D + V  +L +   NI+   +  Y P+H A       +V  L+ N  +++A       P+H   + G+  IV LL+ +  + +   ++G +P H+A + G++ ++  L
Sbjct:  824 CAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 935          
BLAST of SMED30018624 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089172)

HSP 1 Score: 146.362 bits (368), Expect = 4.181e-35
Identity = 110/434 (25.35%), Postives = 189/434 (43.55%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHIGH----HKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL 1417
            +    LL +   N + ++++ + P+H A    NV I  LL++NK D+       + P+H+ACK+G L +  LL+  G  +     DG +P H A   GH+++IK L+   + +  +       +H A +    E A  L+ N   ++     Y   +H+A   G   +  LL+    + NA    G  P+H+AC    ++++E L K   +  +     + P+  A  +G  +IV  LL +  + +  TI  E     HL        I R  +         +E ++PL+V+ + G+++I+ L+L                 AE  I  QS      +    HIA   G  N+VQ LL+ G  +       G   +H+AC +   ++V+ LL      D   K    P+H+    +  SI+ L L    S +L
Sbjct:  214 VNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP--LHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLL--------------QHGAE--INAQS----NDKYSALHIAAKEGQENIVQVLLENGAEN-NAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNL 624          

HSP 2 Score: 125.176 bits (313), Expect = 1.240e-28
Identity = 102/457 (22.32%), Postives = 210/457 (45.95%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----------KEIESP----------LYVSCQRGHLDI-VELILDARLRNV--RNLTNNCHSSAE----DMIKC---QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            G+  E  +LL      ++E+  +    +H A    +V + +LL+  K +  A       P+H+ACK   + +V+LLI +G ++      G +P H+A   G ++I+ +L+ + +  +      +  +H A R N+ +I + L+  +  ++A   +   P+H+A   G  +I  LL+Q   ++NA+++     +H+A   G   I++ L +   + ++       P+  ACK G +++VQ+LL N  + +             F  K     D   +H+  H           K   SP          ++++C++ +L+I ++L+      N+  ++  +  H +A+    DM++      +I    +   T  H+A   GH+ V Q LL+ G ++I     +G   +H+A  + H+D+V+F ++ +   ++ +     P+H      +  I+ L L
Sbjct:  343 GEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASID-------------FQGKN----DVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHG-ANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 116.316 bits (290), Expect = 6.060e-26
Identity = 119/505 (23.56%), Postives = 214/505 (42.38%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLT-RKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL--SDEYKSINSYKK 1453
            +A  +GD+ +V++ L   + ++I   N N    +H A  +  V I   L+     I+    +    +H+A   G  D++  LI    +++V  L+G +P ++A +  H +  + L++NG++  L   + F+   +                           H A + N +  AK L+ ++ N +  +     P+HIA  YG  DI +LL+    DVN    +   P+H+AC  G L +   L       D+A    + P+  A + G   +++ LL  N        N    ++M    EH E  HL    K   +D   +      +  + L+V+   GH+ + +L+LD +   N R L            N     E +IK  + I    E   T  H+A   G +N+V YLL    +  +   + G+  +H+A   +  DI+R LL R+   D + +  Q P+H+       +I+ L L      +   +D+Y +++   K
Sbjct:   44 RAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAP-VDEVTV------DYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQ-HEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAK 539          
BLAST of SMED30018624 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0300497)

HSP 1 Score: 146.362 bits (368), Expect = 4.181e-35
Identity = 110/434 (25.35%), Postives = 189/434 (43.55%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHIGH----HKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL 1417
            +    LL +   N + ++++ + P+H A    NV I  LL++NK D+       + P+H+ACK+G L +  LL+  G  +     DG +P H A   GH+++IK L+   + +  +       +H A +    E A  L+ N   ++     Y   +H+A   G   +  LL+    + NA    G  P+H+AC    ++++E L K   +  +     + P+  A  +G  +IV  LL +  + +  TI  E     HL        I R  +         +E ++PL+V+ + G+++I+ L+L                 AE  I  QS      +    HIA   G  N+VQ LL+ G  +       G   +H+AC +   ++V+ LL      D   K    P+H+    +  SI+ L L    S +L
Sbjct:  214 VNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP--LHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLL--------------QHGAE--INAQS----NDKYSALHIAAKEGQENIVQVLLENGAEN-NAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNL 624          

HSP 2 Score: 125.176 bits (313), Expect = 1.240e-28
Identity = 102/457 (22.32%), Postives = 210/457 (45.95%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----------KEIESP----------LYVSCQRGHLDI-VELILDARLRNV--RNLTNNCHSSAE----DMIKC---QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            G+  E  +LL      ++E+  +    +H A    +V + +LL+  K +  A       P+H+ACK   + +V+LLI +G ++      G +P H+A   G ++I+ +L+ + +  +      +  +H A R N+ +I + L+  +  ++A   +   P+H+A   G  +I  LL+Q   ++NA+++     +H+A   G   I++ L +   + ++       P+  ACK G +++VQ+LL N  + +             F  K     D   +H+  H           K   SP          ++++C++ +L+I ++L+      N+  ++  +  H +A+    DM++      +I    +   T  H+A   GH+ V Q LL+ G ++I     +G   +H+A  + H+D+V+F ++ +   ++ +     P+H      +  I+ L L
Sbjct:  343 GEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASID-------------FQGKN----DVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHG-ANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 116.316 bits (290), Expect = 6.060e-26
Identity = 119/505 (23.56%), Postives = 214/505 (42.38%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLT-RKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL--SDEYKSINSYKK 1453
            +A  +GD+ +V++ L   + ++I   N N    +H A  +  V I   L+     I+    +    +H+A   G  D++  LI    +++V  L+G +P ++A +  H +  + L++NG++  L   + F+   +                           H A + N +  AK L+ ++ N +  +     P+HIA  YG  DI +LL+    DVN    +   P+H+AC  G L +   L       D+A    + P+  A + G   +++ LL  N        N    ++M    EH E  HL    K   +D   +      +  + L+V+   GH+ + +L+LD +   N R L            N     E +IK  + I    E   T  H+A   G +N+V YLL    +  +   + G+  +H+A   +  DI+R LL R+   D + +  Q P+H+       +I+ L L      +   +D+Y +++   K
Sbjct:   44 RAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAP-VDEVTV------DYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQ-HEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAK 539          
BLAST of SMED30018624 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089171)

HSP 1 Score: 146.362 bits (368), Expect = 4.181e-35
Identity = 110/434 (25.35%), Postives = 189/434 (43.55%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KTIYMEHKEVYHLFYSKKTKRIDRFKMHIGH----HKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL 1417
            +    LL +   N + ++++ + P+H A    NV I  LL++NK D+       + P+H+ACK+G L +  LL+  G  +     DG +P H A   GH+++IK L+   + +  +       +H A +    E A  L+ N   ++     Y   +H+A   G   +  LL+    + NA    G  P+H+AC    ++++E L K   +  +     + P+  A  +G  +IV  LL +  + +  TI  E     HL        I R  +         +E ++PL+V+ + G+++I+ L+L                 AE  I  QS      +    HIA   G  N+VQ LL+ G  +       G   +H+AC +   ++V+ LL      D   K    P+H+    +  SI+ L L    S +L
Sbjct:  214 VNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETP--LHLAARANQADIIRILLRSAKVDAIAREGQTPLHVASRLGNINIIMLLL--------------QHGAE--INAQS----NDKYSALHIAAKEGQENIVQVLLENGAEN-NAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNL 624          

HSP 2 Score: 125.176 bits (313), Expect = 1.240e-28
Identity = 102/457 (22.32%), Postives = 210/457 (45.95%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----------KEIESP----------LYVSCQRGHLDI-VELILDARLRNV--RNLTNNCHSSAE----DMIKC---QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            G+  E  +LL      ++E+  +    +H A    +V + +LL+  K +  A       P+H+ACK   + +V+LLI +G ++      G +P H+A   G ++I+ +L+ + +  +      +  +H A R N+ +I + L+  +  ++A   +   P+H+A   G  +I  LL+Q   ++NA+++     +H+A   G   I++ L +   + ++       P+  ACK G +++VQ+LL N  + +             F  K     D   +H+  H           K   SP          ++++C++ +L+I ++L+      N+  ++  +  H +A+    DM++      +I    +   T  H+A   GH+ V Q LL+ G ++I     +G   +H+A  + H+D+V+F ++ +   ++ +     P+H      +  I+ L L
Sbjct:  343 GEHDEAAHLLLDNKAPVDEVTVDYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASID-------------FQGKN----DVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGVISAAAKNGLTPLHVAAQEGHVLVSQILLEHG-ANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 116.316 bits (290), Expect = 6.060e-26
Identity = 119/505 (23.56%), Postives = 214/505 (42.38%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLT-RKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL--SDEYKSINSYKK 1453
            +A  +GD+ +V++ L   + ++I   N N    +H A  +  V I   L+     I+    +    +H+A   G  D++  LI    +++V  L+G +P ++A +  H +  + L++NG++  L   + F+   +                           H A + N +  AK L+ ++ N +  +     P+HIA  YG  DI +LL+    DVN    +   P+H+AC  G L +   L       D+A    + P+  A + G   +++ LL  N        N    ++M    EH E  HL    K   +D   +      +  + L+V+   GH+ + +L+LD +   N R L            N     E +IK  + I    E   T  H+A   G +N+V YLL    +  +   + G+  +H+A   +  DI+R LL R+   D + +  Q P+H+       +I+ L L      +   +D+Y +++   K
Sbjct:   44 RAARSGDIKKVMDFLDCGEISDINSCNANGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLENDVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLDNKAP-VDEVTV------DYLTALHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQ-HEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAK 539          
BLAST of SMED30018624 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 155.606 bits (392), Expect = 7.476e-38
Identity = 114/481 (23.70%), Postives = 204/481 (42.41%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYS--KKTKRI--DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSF 1411
            + V  LL  +  N+    +N   P+H A    NV +V LL+     I+A+   ++ P+H A + G++ +V++L+  G  L     +G SP H+A +  H+D                                 ++K L+  G+   AR  N F+   +H AC+ N +     L+ ++ +LEA       P+H+A   G  +I   L+Q+    NA N     P+H+A   GH  + + L +     D+    +  P+  A ++G K +V+LL+++  N +      H  ++        +T RI  D         K+  +PL+V+C+ G +D+VEL+L+       A    +  L    H +  D++K   L+        T        HIA     L V   LL  G ++   E + G   +H+A      D+V  L+ +    ++ NK    P+H++    +  I  + + +  S 
Sbjct:  222 LSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTP--LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKL--LVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYG-ANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASV 697          

HSP 2 Score: 142.895 bits (359), Expect = 5.753e-34
Identity = 101/456 (22.15%), Postives = 204/456 (44.74%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS--FDLSDEYKSINSYKKSEKLEKA 1474
            +L +   N + +++  + P+H A    N+++ +LL++   ++       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ +++ L+  G+ L+A+       IH A + + ++  + L+  N  ++     +  P+H+A   G   +  +L+ +    NA    G  P+H+AC   H+R ++ L K     ++     + P+  A  +G  +IV+ LL    + N +       ++M     H EV   F  +   ++D         K+ ++PL+ + + GH ++V+L+++ +  N  + T   H+                     HIA   GH    + LLD      +     G   +H+AC +  VD+V  LL+R    +   K    P+H+    +   ++ L +S+  S      + Y +++   K  +LE A
Sbjct:  194 VLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQ-FLLQNNAQVD------AKAKDDQTPLHCAARMGHKELVKLLMEHKA-NPDSATTAGHTP-------------------LHIAAREGHAQTTRILLDENAQQTKMTK-KGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVA 621          

HSP 3 Score: 138.272 bits (347), Expect = 1.552e-32
Identity = 81/302 (26.82%), Postives = 139/302 (46.03%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     N +      + P+H A    +     +L+           +   P+H+ACKYG +D+V+LL+  G + +    +G +P H+A    +LD++K L+S G      ARN ++   +H A + N++E+A  L+    N  + + +   P+H+A + G  D+ +LLI +  +VN  N  G  P+HL    GH+ I + L K      +A+     P+  AC  G   +V+ LL    + N                         K  +G+     +PL+ + Q+GH DIV L+L
Sbjct:  487 ELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTA--LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNS------------------------KTRLGY-----TPLHQAAQQGHTDIVTLLL 757          

HSP 4 Score: 123.635 bits (309), Expect = 4.803e-28
Identity = 102/446 (22.87%), Postives = 186/446 (41.70%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD--ARLR-NVRNLTNNCHSSAE--------DMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGW--------------------------------SDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            I N  DI   N   +  +HLA K G++ +V  L+ NG DL      GN+  H+A   G   ++  LI+ G+++ A++      ++ A + N +E+ K+L+ +  N   P      P+ +A + G E++ +LLI          +YG +       +H+A  N   R    L +   + D  +     P+  A      S+ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ +VE++LD  A L+   +N  +  H +A+         +++  + IDD      T  H+A   GH  +V+ LLD G                                 + +E    SG   +H+A    H++IV+ LL R    +  N + + P+HM   + +  +    L  N   D
Sbjct:   35 IKNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLI----------NYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVN---FTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVD 467          

HSP 5 Score: 121.709 bits (304), Expect = 1.847e-27
Identity = 107/485 (22.06%), Postives = 207/485 (42.68%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +A  +G+L + L+ + +   +I   N+N    +H A    +V +V  L+ N  D+E    +    +H+A   G   +V  LI+ G +++     G SP ++A +  HL+++K+L+ +G++  L   + F+   +                           H A R +    A  L+ N+ N +  +     P+HIA  Y    +  LL+ +  +VN     G  P+H+A   G++ ++  L       D+     + P+  A + G   +V++LLD               +  +  + +   +D  +  + ++ EI+       +PL+V+   GH  +V+++LD   + N R L            N   S + ++K  + ++   E   T  H+A   GHLN+V+ LL  G S     ++  +  +H+A    H ++ +FLL  N   D   K  Q P+H      +  ++ L +    + D
Sbjct:   21 RAARSGNLEKALDHI-KNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKT---KNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASP-NASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPD 500          

HSP 6 Score: 102.834 bits (255), Expect = 1.162e-21
Identity = 54/205 (26.34%), Postives = 103/205 (50.24%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHL 745
            ++V+ LL  K  +      N Y  +H A   + + +   L+    +  +E+ Q + P+HLA + G  D+V LLIS   ++++ + +G +P HL  + GH+ I   L+  G+ + A +      +H AC +  I++ KFL+    ++ +       P+H A + G  DI +LL++     N   + G  P+ +A   G++ +I+ L
Sbjct:  585 LDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 789          
BLAST of SMED30018624 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 155.221 bits (391), Expect = 7.688e-38
Identity = 115/482 (23.86%), Postives = 206/482 (42.74%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSK---KTKRI--DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSF 1411
            + V  LL  +  N+    +N   P+H A    NV +V LL+     I+A+   ++ P+H A + G++ +V++L+  G  L     +G SP H+A +  H+D                                 ++K L+  G+   AR  N F+   +H AC+ N +     L+ ++ +LEA       P+H+A   G  +I   L+Q+    NA N     P+H+A   GH  + + L +     D+    +  P+  A ++G K +V+LL+++  N +      H  + H+   +   +T RI  D         K+  +PL+V+C+ G +D+VEL+L+       A    +  L    H +  D++K   L+        T        HIA     L V   LL  G ++   E + G   +H+A      D+V  L+ +    ++ NK    P+H++    +  I  + + +  S 
Sbjct:  160 LSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTP--LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPL-HIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKL--LVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYG-ANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASV 635          

HSP 2 Score: 142.124 bits (357), Expect = 7.730e-34
Identity = 101/456 (22.15%), Postives = 205/456 (44.96%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS--DEYKSINSYKKSEKLEKA 1474
            +L +   N + +++  + P+H A    N+++ +LL++   ++       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ +++ L+  G+ L+A+       IH A + + ++  + L+  N  ++     +  P+H+A   G   +  +L+ +    NA    G  P+H+AC   H+R ++ L K     ++     + P+  A  +G  +IV+ LL    + N +       ++M     H EV   F  +   ++D         K+ ++PL+ + + GH ++V+L+++ +  N  + T   H+                     HIA   GH    + LLD      +     G   +H+AC +  VD+V  LL+R    +   K    P+H+    +   ++ L +S+  S   +  + Y +++   K  +LE A
Sbjct:  132 VLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQ-FLLQNNAQVD------AKAKDDQTPLHCAARMGHKELVKLLMEHK-ANPDSATTAGHTP-------------------LHIAAREGHAQTTRILLDENAQQTKMTK-KGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVA 559          

HSP 3 Score: 137.887 bits (346), Expect = 1.541e-32
Identity = 81/302 (26.82%), Postives = 139/302 (46.03%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     N +      + P+H A    +     +L+           +   P+H+ACKYG +D+V+LL+  G + +    +G +P H+A    +LD++K L+S G      ARN ++   +H A + N++E+A  L+    N  + + +   P+H+A + G  D+ +LLI +  +VN  N  G  P+HL    GH+ I + L K      +A+     P+  AC  G   +V+ LL    + N                         K  +G+     +PL+ + Q+GH DIV L+L
Sbjct:  425 ELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTA--LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNS------------------------KTRLGY-----TPLHQAAQQGHTDIVTLLL 695          

HSP 4 Score: 112.464 bits (280), Expect = 1.150e-24
Identity = 96/438 (21.92%), Postives = 184/438 (42.01%), Query Frame = 2
Query:  248 LISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            L+ N  D+E    +    +H+A   G   +V  LI+ G +++     G SP ++A +  HL+++K+L+ +G++  L   + F+   +                           H A R +    A  L+ N+ N +  +     P+HIA  Y    +  LL+ +  +VN     G  P+H+A   G++ ++  L       D+     + P+  A + G   +V++LLD               +  +  + +   +D  +  + ++ EI+       +PL+V+   GH  +V+++LD   + N R L            N   S + ++K  + ++   E   T  H+A   GHLN+V+ LL  G S     ++  +  +H+A    H ++ +FLL  N   D   K  Q P+H      +  ++ L +    + D
Sbjct:    5 LLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTK---NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASP-NASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPD 438          

HSP 5 Score: 102.449 bits (254), Expect = 1.561e-21
Identity = 54/205 (26.34%), Postives = 103/205 (50.24%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHL 745
            ++V+ LL  K  +      N Y  +H A   + + +   L+    +  +E+ Q + P+HLA + G  D+V LLIS   ++++ + +G +P HL  + GH+ I   L+  G+ + A +      +H AC +  I++ KFL+    ++ +       P+H A + G  DI +LL++     N   + G  P+ +A   G++ +I+ L
Sbjct:  523 LDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 727          
BLAST of SMED30018624 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 154.836 bits (390), Expect = 9.204e-38
Identity = 115/482 (23.86%), Postives = 206/482 (42.74%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSK---KTKRI--DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSF 1411
            + V  LL  +  N+    +N   P+H A    NV +V LL+     I+A+   ++ P+H A + G++ +V++L+  G  L     +G SP H+A +  H+D                                 ++K L+  G+   AR  N F+   +H AC+ N +     L+ ++ +LEA       P+H+A   G  +I   L+Q+    NA N     P+H+A   GH  + + L +     D+    +  P+  A ++G K +V+LL+++  N +      H  + H+   +   +T RI  D         K+  +PL+V+C+ G +D+VEL+L+       A    +  L    H +  D++K   L+        T        HIA     L V   LL  G ++   E + G   +H+A      D+V  L+ +    ++ NK    P+H++    +  I  + + +  S 
Sbjct:  252 LSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTP--LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPL-HIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKL--LVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYG-ANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASV 727          

HSP 2 Score: 142.124 bits (357), Expect = 7.787e-34
Identity = 101/456 (22.15%), Postives = 204/456 (44.74%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS--FDLSDEYKSINSYKKSEKLEKA 1474
            +L +   N + +++  + P+H A    N+++ +LL++   ++       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ +++ L+  G+ L+A+       IH A + + ++  + L+  N  ++     +  P+H+A   G   +  +L+ +    NA    G  P+H+AC   H+R ++ L K     ++     + P+  A  +G  +IV+ LL    + N +       ++M     H EV   F  +   ++D         K+ ++PL+ + + GH ++V+L+++ +  N  + T   H+                     HIA   GH    + LLD      +     G   +H+AC +  VD+V  LL+R    +   K    P+H+    +   ++ L +S+  S      + Y +++   K  +LE A
Sbjct:  224 VLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQ-FLLQNNAQVD------AKAKDDQTPLHCAARMGHKELVKLLMEHK-ANPDSATTAGHTP-------------------LHIAAREGHAQTTRILLDENAQQTKMTK-KGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVA 651          

HSP 3 Score: 137.887 bits (346), Expect = 2.084e-32
Identity = 87/313 (27.80%), Postives = 147/313 (46.96%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDN----NCNTNKTIYMEHKEVYHLF-YSKKTKRIDRFK------MHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     N +      + P+H A    +     +L+           +   P+H+ACKYG +D+V+LL+  G + +    +G +P H+A    +LD++K L+S G      ARN ++   +H A + N++E+A  L+    N  + + +   P+H+A + G  D+ +LLI +  +VN  N  G  P+HL    GH+ I + L K      +A+     P+  AC  G   +V+ LL      N  T  ++ +       LF  S  T R   F         +G+     +PL+ + Q+GH DIV L+L
Sbjct:  517 ELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTA--LHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRVSVLLLDASYTGLFSVSVCTLRTFAFSFSLIFLFQLGY-----TPLHQAAQQGHTDIVTLLL 822          

HSP 4 Score: 124.02 bits (310), Expect = 3.497e-28
Identity = 102/446 (22.87%), Postives = 186/446 (41.70%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD--ARLR-NVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGW--------------------------------SDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            I N  DI   N   +  +HLA K G++ +V  L+ NG DL      GN+  H+A   G   ++  LI+ G+++ A++      ++ A + N +E+ K+L+ +  N   P      P+ +A + G E++ +LLI          +YG +       +H+A  N   R    L +   + D  +     P+  A      S+ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ +VE++LD  A L+   +N  +  H +A+         +++  + IDD      T  H+A   GH  +V+ LLD G                                 + +E    SG   +H+A    H++IV+ LL R    +  N + + P+HM   + +  +    L  N   D
Sbjct:   65 IKNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLI----------NYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVN---FTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVD 497          

HSP 5 Score: 120.939 bits (302), Expect = 3.119e-27
Identity = 107/485 (22.06%), Postives = 207/485 (42.68%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +A  +G+L + L+ + +   +I   N+N    +H A    +V +V  L+ N  D+E    +    +H+A   G   +V  LI+ G +++     G SP ++A +  HL+++K+L+ +G++  L   + F+   +                           H A R +    A  L+ N+ N +  +     P+HIA  Y    +  LL+ +  +VN     G  P+H+A   G++ ++  L       D+     + P+  A + G   +V++LLD               ++    + +   +D  +  + ++ EI+       +PL+V+   GH  +V+++LD   + N R L            N   S + ++K  + ++   E   T  H+A   GHLN+V+ LL  G S     ++  +  +H+A    H ++ +FLL  N   D   K  Q P+H      +  ++ L +    + D
Sbjct:   51 RAARSGNLEKALDHI-KNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIH---MAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASP-NASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPD 530          

HSP 6 Score: 94.3597 bits (233), Expect = 4.400e-19
Identity = 71/310 (22.90%), Postives = 127/310 (40.97%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAEN---------SY--------------------------GNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRI 934
            AC  G  ++V+ LL  +  N     +N   P+H A+ ++N+ +V+LL+S      +        +H+A K   L++   L+  G + +   L G +P HLA + G  D++  LIS  +++   N      +H   +   + IA  L+    ++ A +     P+H+AC YG   +   L+QQ   VN++          SY                          G  P+H A   GH  I+  L K     +        P+  A +LG  S++ +L      T +++ M   E + + + +    I
Sbjct:  576 ACKYGK-VDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRVSVLLLDASYTGLFSVSVCTLRTFAFSFSLIFLFQLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL---KLVTEESVSMITTEKHRMSFPETVDEI 881          
BLAST of SMED30018624 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 153.68 bits (387), Expect = 3.270e-37
Identity = 99/430 (23.02%), Postives = 193/430 (44.88%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +V  ++  K  N      N + P+H A   + V ++ELL+ +   ++A  +  + PIH+A   G+ +IV+ L  +G   +  ++ G +  H+A   G +D++++L+ NG+ ++ +    Q  +H A R  ++EI + L+       A       P+H++   G ++I +LL++Q   ++A    G  P+H+A   G L +   L + K   D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  SPL+++ Q G +D+V L+L  +  NV N+ N    +                    H+A       V + LL+ G ++I+ +  SG   +H+AC + ++ +  FLL+     +   K    P+H      +  I+ + L    S
Sbjct:  382 KVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPL-HIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLL-TKQANV-NMGNKNGLTP------------------LHLAAQDDKAGVTEVLLNHG-AEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGAS 789          

HSP 2 Score: 125.946 bits (315), Expect = 1.099e-28
Identity = 90/428 (21.03%), Postives = 184/428 (42.99%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKC--DIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            LL +   N +  +++ + P+H A    N+ +  LL++     D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+  G+ + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +++ +  + NA+   G  P+H+AC    ++++E L K      +     + PI  A  +G ++IV+ L  +  + N T  +  +   H+        + R+ +  G       K+ ++ L+++ + G L+IV+ +L         L N   +S    +               H++   GH  +   LL+ G S +      G   +H+A  +  +++   LL +    D   K    P+H+        +  L L +  S
Sbjct:  221 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARND--ITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPN-TTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGA-----LPNAATTSGYTPL---------------HLSAREGHQEIAALLLEQG-SSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGAS 624          

HSP 3 Score: 121.324 bits (303), Expect = 3.001e-27
Identity = 113/484 (23.35%), Postives = 211/484 (43.60%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTI-----------YMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL--SDEYKSINSYKKSEKLE 1468
            +A   G+L +VL+ L +   +I   N+N    +H A    +V +V  L+    +++A   +    +H+A   G  ++V+ L++NG +++    +G +P ++A +  HLD+++FL+ N S   +   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD                       H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L      V ++TN+     H +A     K   +I D+K          F   HIAC    + V++ LL  G S ++    SG   IH+A    H +IV+ L       +  N R +  +HM   +    ++   L      D+   D+  +++   +  KLE
Sbjct:   48 RAARAGNLEKVLDYL-KTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEML-------LDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNA-PVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGAS-LQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLE 514          

HSP 4 Score: 117.857 bits (294), Expect = 3.733e-26
Identity = 99/472 (20.97%), Postives = 189/472 (40.04%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE-----------------------------------ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            I V  LL  +   ++ +  N   P+H A    N  +V+LL+     IEA+    + P+H   + G+  +V++L+  G  +     +G SP H+A +  HL+ ++ L+ + + ++                                   A N F+   +H AC+ NR+++ + L+ +  +L+A       PIH+A   G E+I   L       N  N  G   +H+A   G + ++ +L +     D     +   +  A +LG   IVQ LL      N      +  + HL   +  + I    +  G       K+  +PL+V+ + G L++  L+L  +             +A D      L          H+A    +  V   LLD G S       +G   +HIA   + ++I   LL+     + + ++   P+H+        ++ L L++  + ++ ++
Sbjct:  249 INVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTP--LHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPL-HLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKK-------------AAPDAAGKSGLT-------PLHVAAHYDNQRVALLLLDQGASP-HSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNK 696          

HSP 5 Score: 96.6709 bits (239), Expect = 9.663e-20
Identity = 78/348 (22.41%), Postives = 140/348 (40.23%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   G+L+ +   +  G D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++   L+    +VNA++  G  P+++A    HL ++  L +       A      P+  A + G   +V LLL+N+      +        H+   K   +     +   H+ ++ES     PL+++   G++++  L+L+               +A D +    +          H+A   G+ N+V+ LLD G S IE +   G   +H      H  +V  LLDR        K    P+HM     + + + L L  N   D
Sbjct:   41 DSNASYLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RGAAVDFMARNDIT-------PLHVASKRGNGNMVKLLLDRG-SKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVD 362          

HSP 6 Score: 93.5893 bits (231), Expect = 1.006e-18
Identity = 50/246 (20.33%), Postives = 109/246 (44.31%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            E+  LL  + +++    +  + P+H A     + +  LL+  K   +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   L+I   L+  G++           +H A +   +++   L++   N+   N     P+H+A +     +  +L+    +++A+   G  P+H+AC  G++++   L + +   ++       P+  A + G   I+ +LL    + N+
Sbjct:  547 EIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNE 792          

HSP 7 Score: 90.8929 bits (224), Expect = 5.630e-18
Identity = 54/222 (24.32%), Postives = 108/222 (48.65%), Query Frame = 2
Query:  185 ENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLD 850
            +N Y P+H A   + + I   L+    +      Q + P+HLA + G++D+V LL++   ++++ + +G +P HLA +     + + L+++G++++A+       +H AC +  +++A FL+ N     A       P+H A + G   I ++L+Q     N     GN  + +A   G++ +++ L  V  +  +A      PI    K+ V   +   LD
Sbjct:  630 KNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTLRGVTDENLTAT-----PITEKHKINVPETMNEFLD 846          
BLAST of SMED30018624 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 153.68 bits (387), Expect = 3.270e-37
Identity = 99/430 (23.02%), Postives = 193/430 (44.88%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +V  ++  K  N      N + P+H A   + V ++ELL+ +   ++A  +  + PIH+A   G+ +IV+ L  +G   +  ++ G +  H+A   G +D++++L+ NG+ ++ +    Q  +H A R  ++EI + L+       A       P+H++   G ++I +LL++Q   ++A    G  P+H+A   G L +   L + K   D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  SPL+++ Q G +D+V L+L  +  NV N+ N    +                    H+A       V + LL+ G ++I+ +  SG   +H+AC + ++ +  FLL+     +   K    P+H      +  I+ + L    S
Sbjct:  382 KVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPL-HIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLL-TKQANV-NMGNKNGLTP------------------LHLAAQDDKAGVTEVLLNHG-AEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGAS 789          

HSP 2 Score: 125.946 bits (315), Expect = 1.099e-28
Identity = 90/428 (21.03%), Postives = 184/428 (42.99%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKC--DIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            LL +   N +  +++ + P+H A    N+ +  LL++     D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+  G+ + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +++ +  + NA+   G  P+H+AC    ++++E L K      +     + PI  A  +G ++IV+ L  +  + N T  +  +   H+        + R+ +  G       K+ ++ L+++ + G L+IV+ +L         L N   +S    +               H++   GH  +   LL+ G S +      G   +H+A  +  +++   LL +    D   K    P+H+        +  L L +  S
Sbjct:  221 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARND--ITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPN-TTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGA-----LPNAATTSGYTPL---------------HLSAREGHQEIAALLLEQG-SSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGAS 624          

HSP 3 Score: 121.324 bits (303), Expect = 3.001e-27
Identity = 113/484 (23.35%), Postives = 211/484 (43.60%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTI-----------YMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL--SDEYKSINSYKKSEKLE 1468
            +A   G+L +VL+ L +   +I   N+N    +H A    +V +V  L+    +++A   +    +H+A   G  ++V+ L++NG +++    +G +P ++A +  HLD+++FL+ N S   +   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD                       H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L      V ++TN+     H +A     K   +I D+K          F   HIAC    + V++ LL  G S ++    SG   IH+A    H +IV+ L       +  N R +  +HM   +    ++   L      D+   D+  +++   +  KLE
Sbjct:   48 RAARAGNLEKVLDYL-KTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEML-------LDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNA-PVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGAS-LQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLE 514          

HSP 4 Score: 117.857 bits (294), Expect = 3.733e-26
Identity = 99/472 (20.97%), Postives = 189/472 (40.04%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE-----------------------------------ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            I V  LL  +   ++ +  N   P+H A    N  +V+LL+     IEA+    + P+H   + G+  +V++L+  G  +     +G SP H+A +  HL+ ++ L+ + + ++                                   A N F+   +H AC+ NR+++ + L+ +  +L+A       PIH+A   G E+I   L       N  N  G   +H+A   G + ++ +L +     D     +   +  A +LG   IVQ LL      N      +  + HL   +  + I    +  G       K+  +PL+V+ + G L++  L+L  +             +A D      L          H+A    +  V   LLD G S       +G   +HIA   + ++I   LL+     + + ++   P+H+        ++ L L++  + ++ ++
Sbjct:  249 INVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTP--LHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPL-HLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKK-------------AAPDAAGKSGLT-------PLHVAAHYDNQRVALLLLDQGASP-HSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNK 696          

HSP 5 Score: 96.6709 bits (239), Expect = 9.663e-20
Identity = 78/348 (22.41%), Postives = 140/348 (40.23%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   G+L+ +   +  G D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++   L+    +VNA++  G  P+++A    HL ++  L +       A      P+  A + G   +V LLL+N+      +        H+   K   +     +   H+ ++ES     PL+++   G++++  L+L+               +A D +    +          H+A   G+ N+V+ LLD G S IE +   G   +H      H  +V  LLDR        K    P+HM     + + + L L  N   D
Sbjct:   41 DSNASYLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RGAAVDFMARNDIT-------PLHVASKRGNGNMVKLLLDRG-SKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVD 362          

HSP 6 Score: 93.5893 bits (231), Expect = 1.006e-18
Identity = 50/246 (20.33%), Postives = 109/246 (44.31%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            E+  LL  + +++    +  + P+H A     + +  LL+  K   +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   L+I   L+  G++           +H A +   +++   L++   N+   N     P+H+A +     +  +L+    +++A+   G  P+H+AC  G++++   L + +   ++       P+  A + G   I+ +LL    + N+
Sbjct:  547 EIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNE 792          

HSP 7 Score: 90.8929 bits (224), Expect = 5.630e-18
Identity = 54/222 (24.32%), Postives = 108/222 (48.65%), Query Frame = 2
Query:  185 ENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLD 850
            +N Y P+H A   + + I   L+    +      Q + P+HLA + G++D+V LL++   ++++ + +G +P HLA +     + + L+++G++++A+       +H AC +  +++A FL+ N     A       P+H A + G   I ++L+Q     N     GN  + +A   G++ +++ L  V  +  +A      PI    K+ V   +   LD
Sbjct:  630 KNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTLRGVTDENLTAT-----PITEKHKINVPETMNEFLD 846          
BLAST of SMED30018624 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 154.451 bits (389), Expect = 2.281e-37
Identity = 100/407 (24.57%), Postives = 181/407 (44.47%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N + P+H A   S++ ++ELL+ +   I+A  +  + P+H+A   G+L IV+ L+  G   +V  +   +P H+A    H D+  FLI N + + AR    Q  +H A R    E+ + L+ N  + + P      P+H+    G      LL+       +    G  P+H+A   G ++I E L + +   +SA    + P+  A      +IV+LLL+   + + T    +  + H+   +++    R  +  G     +S     PL+++ Q GH D+V L+L  +         N H            + ++      H+A   GHLN+ + L+    S +  E   G   +H+AC + ++ +V+FLL      + + K    P+H      +  I+ L L
Sbjct:  361 NGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPL-HMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQA--------NVH------------LGNKSSLTPLHLASQEGHLNIAEVLVSHDAS-VAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 745          

HSP 2 Score: 144.05 bits (362), Expect = 4.109e-34
Identity = 109/445 (24.49%), Postives = 198/445 (44.49%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKR----IDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A   G++I V  LL R    I+   ++   P+H A+ N +V I E+L+ +   I+A+    + PIH+A +  +LD V+LL+     +    LD  +P H+A   GH  + K L+  G+   AR  N F+   +H AC+ + I + + L+ +  +++A       P+H+A   G   I   L+Q+    N  +     P+H+A    H  + + L + K   ++    +  P+  A + G   +V+LLL+N  + +      H  ++       T+     +D    H    K+  +PL+V+ + G + I EL+L+             H ++        L          H+A    HLN+V+ LL+ G S   C   +G   +H+A     VD  R LL      +  + +   P+H+     +  ++ L LS+  +  L ++
Sbjct:  237 ASRRGNVIMVRLLLDRGA-QIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP--LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE----------REAHPNSAGKTGLTPL----------HVAVYHNHLNIVRLLLNKGASP-HCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNK 657          

HSP 3 Score: 119.013 bits (297), Expect = 1.920e-26
Identity = 100/460 (21.74%), Postives = 194/460 (42.17%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD----------------LEARNNFSQYLI-------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASI 1378
            +A   G+L + L+ L R   +I   N+N    +H +    +V +V  L+     +E    +    +H+A   G  D+V+ L+++G +++     G +P ++A +  HL ++KFL+ NG++                 +   N   YLI             H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +  +VN     G   +H+A   G++ ++  L       D+     + P+  A + G   I ++LLD+              ++    + +   +D  ++ + +   I+       +PL+V+   GH  + +L+LD              S+A  +     L          HIAC   H+ V++ LL  G S I+    SG   +H+A    H+ IV+ LL +    ++ + + + P+HM   +++  +
Sbjct:    9 RAARAGNLDKALDHL-RNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIH---MASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD----------KGAKSNARALNGFTPL----------HIACKKSHIRVMELLLKSGAS-IDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDV 443          

HSP 4 Score: 116.701 bits (291), Expect = 9.536e-26
Identity = 75/302 (24.83%), Postives = 131/302 (43.38%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE--ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     + +      + P+H      +    ELL+ N+    +   +   P+H+A KYG + I +LL+      +     G +P H+A    HL+I++ L++ G+     ARN ++   +H A +   ++ A+ L+       A + +   P+H+A + G  D+ +LL+ +  +V+  N     P+HLA   GHL I E L        +       P+  AC  G   +V+ LL +  N N         V  L +                     +PL+ + Q+GH DIV L+L
Sbjct:  475 EMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP--LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNA--------VTKLGF---------------------TPLHQAAQQGHTDIVTLLL 745          

HSP 5 Score: 108.997 bits (271), Expect = 2.440e-23
Identity = 94/414 (22.71%), Postives = 177/414 (42.75%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD------ARLRNVRNLTNNCHSSAEDMIKC-------QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            + N  DI   N   +  +HL+ K G++ +V  L+     L      GN+  H+A   G  D+++ L+++G+++ +++      ++ A + N + + KFL+ N  N          P+ +A + G E++ + LI          SYG +       +H+A  N   R    L +   + D  +     P+  A      ++ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ I E++LD      A+ +N   L+    +S  D + C       Q+ IDD      T  H+A   GH  V + LLD G +      ++G   +HIAC  SH+ ++  LL      D + +    P+H+     +  I+   L +  S
Sbjct:   23 LRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLI----------SYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVN---FTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKN--GLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKG-AKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGAS 420          

HSP 6 Score: 105.916 bits (263), Expect = 2.101e-22
Identity = 57/207 (27.54%), Postives = 101/207 (48.79%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFK 751
            I++  LL  +  +     +    P+H A+ ++++ IV LL++              P+H+A K  ++D  + L+  G   +   L G +P HLA + GH D++  L+S  +++   N  S   +H A +   + IA+ L+S++ ++ A       P+H+AC YG   +   L+Q   +VNA    G  P+H A   GH  I+  L K
Sbjct:  540 IQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLK 746          

HSP 7 Score: 104.375 bits (259), Expect = 5.638e-22
Identity = 92/458 (20.09%), Postives = 178/458 (38.86%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD-------GNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +V+  L     N+   ++  + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+ ++V  LIS G    V                      ND +       G +P H+A  + +L++ + L++ G+++          +H A R   + + + L+     ++A       P+H A   G   I  +L+     + A+   G  PIH+A    HL  +  L + +   D     ++ P+  A   G   + +LLLD    +N                   + ++ F           +PL+++C++ H+ ++EL+L                      K  + ID   E   T  H+A   GHL +V+ LL  G S      +  +  +H+A   +H D+  FL+      +   K  Q P+H    S +  ++ L L      D
Sbjct:   83 DVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSN------------------ARALNGF-----------TPLHIACKKSHIRVMELLL----------------------KSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASP-NVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPD 488          

HSP 8 Score: 104.375 bits (259), Expect = 5.783e-22
Identity = 56/227 (24.67%), Postives = 110/227 (48.46%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT 874
            + P+H A     + I ELL+  +    +     + P+H+A  + +L+IV+LL++ G        +G +P H+A +   +D  + L+  G+   A++      +H A +    ++   L+S   N+   N     P+H+A + G  +I  +L+  +  V AE   G  P+H+AC  G++++++ L + + + ++       P+  A + G   IV LLL N    N+ 
Sbjct:  528 FTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQA 754          

HSP 9 Score: 95.1301 bits (235), Expect = 3.828e-19
Identity = 50/177 (28.25%), Postives = 90/177 (50.85%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNC 661
            + ++ LL  K  +      N Y P+H A+   +V     L+       A++ Q + P+HLA + G+ D+V LL+S   ++ + +    +P HLA + GHL+I + L+S+ + + A        +H AC +  I++ KFL+ +  N+ A       P+H A + G  DI +LL++   
Sbjct:  573 LNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGA 749          

HSP 10 Score: 78.5666 bits (192), Expect = 4.337e-14
Identity = 77/351 (21.94%), Postives = 150/351 (42.74%), Query Frame = 2
Query:  410 ACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNV----RNLTNNCH--SSAEDMIKCQSLIDDEKE-RFCT-------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            A   G+LD     + NG D+   N      +H + +   +++   L+  +  LE    K +  +HIA   G ED+   L+    +VN+++  G  P+++A    HL +++ L +   + + A      P+  A + G +++V  L+         +        H+       R     +    + ++ S     PL+++    +L++ +L+L+ R  NV    +N  +  H  S   ++I  + L+D   +   CT       H A  +GH+ + + LLD G + I+ +  +G   IH+A    H+D VR LL      D +   +  P+H+     +  +  L L +  
Sbjct:   10 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRL-----PALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLN-RGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHG-APIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGA 353          
BLAST of SMED30018624 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 154.451 bits (389), Expect = 2.658e-37
Identity = 100/407 (24.57%), Postives = 181/407 (44.47%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N + P+H A   S++ ++ELL+ +   I+A  +  + P+H+A   G+L IV+ L+  G   +V  +   +P H+A    H D+  FLI N + + AR    Q  +H A R    E+ + L+ N  + + P      P+H+    G      LL+       +    G  P+H+A   G ++I E L + +   +SA    + P+  A      +IV+LLL+   + + T    +  + H+   +++    R  +  G     +S     PL+++ Q GH D+V L+L  +         N H            + ++      H+A   GHLN+ + L+    S +  E   G   +H+AC + ++ +V+FLL      + + K    P+H      +  I+ L L
Sbjct:  403 NGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPL-HMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQA--------NVH------------LGNKSSLTPLHLASQEGHLNIAEVLVSHDAS-VAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 787          

HSP 2 Score: 143.28 bits (360), Expect = 7.521e-34
Identity = 109/445 (24.49%), Postives = 198/445 (44.49%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKR----IDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A   G++I V  LL R    I+   ++   P+H A+ N +V I E+L+ +   I+A+    + PIH+A +  +LD V+LL+     +    LD  +P H+A   GH  + K L+  G+   AR  N F+   +H AC+ + I + + L+ +  +++A       P+H+A   G   I   L+Q+    N  +     P+H+A    H  + + L + K   ++    +  P+  A + G   +V+LLL+N  + +      H  ++       T+     +D    H    K+  +PL+V+ + G + I EL+L+             H ++        L          H+A    HLN+V+ LL+ G S   C   +G   +H+A     VD  R LL      +  + +   P+H+     +  ++ L LS+  +  L ++
Sbjct:  279 ASRRGNVIMVRLLLDRGA-QIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP--LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE----------REAHPNSAGKTGLTPL----------HVAVYHNHLNIVRLLLNKGASP-HCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNK 699          

HSP 3 Score: 118.627 bits (296), Expect = 2.741e-26
Identity = 102/461 (22.13%), Postives = 197/461 (42.73%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD----------------LEARNNFSQYLI-------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASI 1378
            +A   G+L + L+ L R   +I   N+N    +H +    +V +V  L+     +E    +    +H+A   G  D+V+ L+++G +++     G +P ++A +  HL ++KFL+ NG++                 +   N   YLI             H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +  +VN     G   +H+A   G++ ++  L       D+     + P+  A + G   I ++LLD+       I  + K  +  +  + +   +D  ++ + +   I+       +PL+V+   GH  + +L+LD              S+A  +     L          HIAC   H+ V++ LL  G S I+    SG   +H+A    H+ IV+ LL +    ++ + + + P+HM   +++  +
Sbjct:   51 RAARAGNLDKALDHL-RNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGA----PIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD----------KGAKSNARALNGFTPL----------HIACKKSHIRVMELLLKSGAS-IDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDV 485          

HSP 4 Score: 116.701 bits (291), Expect = 9.664e-26
Identity = 75/302 (24.83%), Postives = 131/302 (43.38%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     + +      + P+H      +    ELL+ N+    +   +   P+H+A KYG + I +LL+      +     G +P H+A    HL+I++ L++ G+     ARN ++   +H A +   ++ A+ L+       A + +   P+H+A + G  D+ +LL+ +  +V+  N     P+HLA   GHL I E L        +       P+  AC  G   +V+ LL +  N N         V  L +                     +PL+ + Q+GH DIV L+L
Sbjct:  517 EMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP--LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNA--------VTKLGF---------------------TPLHQAAQQGHTDIVTLLL 787          

HSP 5 Score: 112.849 bits (281), Expect = 1.434e-24
Identity = 64/238 (26.89%), Postives = 115/238 (48.32%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            I++  LL  +  +     +    P+H A+ ++++ IV LL++              P+H+A K  ++D  + L+  G   +   L G +P HLA + GH D++  L+S  +++   N  S   +H A +   + IA+ L+S++ ++ A       P+H+AC YG   +   L+Q   +VNA    G  P+H A   GH  I+  L K     + A+   + P+  A +LG  S+  +L
Sbjct:  582 IQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 819          

HSP 6 Score: 108.612 bits (270), Expect = 3.196e-23
Identity = 94/414 (22.71%), Postives = 177/414 (42.75%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD------ARLRNVRNLTNNCHSSAEDMIKC-------QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            + N  DI   N   +  +HL+ K G++ +V  L+     L      GN+  H+A   G  D+++ L+++G+++ +++      ++ A + N + + KFL+ N  N          P+ +A + G E++ + LI          SYG +       +H+A  N   R    L +   + D  +     P+  A      ++ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ I E++LD      A+ +N   L+    +S  D + C       Q+ IDD      T  H+A   GH  V + LLD G +      ++G   +HIAC  SH+ ++  LL      D + +    P+H+     +  I+   L +  S
Sbjct:   65 LRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLI----------SYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVN---FTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKN--GLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKG-AKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGAS 462          

HSP 7 Score: 103.99 bits (258), Expect = 7.872e-22
Identity = 92/458 (20.09%), Postives = 178/458 (38.86%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD-------GNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +V+  L     N+   ++  + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+ ++V  LIS G    V                      ND +       G +P H+A  + +L++ + L++ G+++          +H A R   + + + L+     ++A       P+H A   G   I  +L+     + A+   G  PIH+A    HL  +  L + +   D     ++ P+  A   G   + +LLLD    +N                   + ++ F           +PL+++C++ H+ ++EL+L                      K  + ID   E   T  H+A   GHL +V+ LL  G S      +  +  +H+A   +H D+  FL+      +   K  Q P+H    S +  ++ L L      D
Sbjct:  125 DVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSN------------------ARALNGF-----------TPLHIACKKSHIRVMELLL----------------------KSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASP-NVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPD 530          

HSP 8 Score: 103.99 bits (258), Expect = 9.095e-22
Identity = 56/226 (24.78%), Postives = 110/226 (48.67%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + I ELL+  +    +     + P+H+A  + +L+IV+LL++ G        +G +P H+A +   +D  + L+  G+   A++      +H A +    ++   L+S   N+   N     P+H+A + G  +I  +L+  +  V AE   G  P+H+AC  G++++++ L + + + ++       P+  A + G   IV LLL N    N+
Sbjct:  570 FTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQ 795          

HSP 9 Score: 78.5666 bits (192), Expect = 5.587e-14
Identity = 77/351 (21.94%), Postives = 150/351 (42.74%), Query Frame = 2
Query:  410 ACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNV----RNLTNNCH--SSAEDMIKCQSLIDDEKE-RFCT-------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            A   G+LD     + NG D+   N      +H + +   +++   L+  +  LE    K +  +HIA   G ED+   L+    +VN+++  G  P+++A    HL +++ L +   + + A      P+  A + G +++V  L+         +        H+       R     +    + ++ S     PL+++    +L++ +L+L+ R  NV    +N  +  H  S   ++I  + L+D   +   CT       H A  +GH+ + + LLD G + I+ +  +G   IH+A    H+D VR LL      D +   +  P+H+     +  +  L L +  
Sbjct:   52 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRL-----PALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLN-RGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHG-APIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGA 395          
BLAST of SMED30018624 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 154.066 bits (388), Expect = 2.937e-37
Identity = 100/407 (24.57%), Postives = 181/407 (44.47%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N + P+H A   S++ ++ELL+ +   I+A  +  + P+H+A   G+L IV+ L+  G   +V  +   +P H+A    H D+  FLI N + + AR    Q  +H A R    E+ + L+ N  + + P      P+H+    G      LL+       +    G  P+H+A   G ++I E L + +   +SA    + P+  A      +IV+LLL+   + + T    +  + H+   +++    R  +  G     +S     PL+++ Q GH D+V L+L  +         N H            + ++      H+A   GHLN+ + L+    S +  E   G   +H+AC + ++ +V+FLL      + + K    P+H      +  I+ L L
Sbjct:  403 NGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPL-HMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQA--------NVH------------LGNKSSLTPLHLASQEGHLNIAEVLVSHDAS-VAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 787          

HSP 2 Score: 143.28 bits (360), Expect = 7.828e-34
Identity = 109/445 (24.49%), Postives = 198/445 (44.49%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKR----IDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A   G++I V  LL R    I+   ++   P+H A+ N +V I E+L+ +   I+A+    + PIH+A +  +LD V+LL+     +    LD  +P H+A   GH  + K L+  G+   AR  N F+   +H AC+ + I + + L+ +  +++A       P+H+A   G   I   L+Q+    N  +     P+H+A    H  + + L + K   ++    +  P+  A + G   +V+LLL+N  + +      H  ++       T+     +D    H    K+  +PL+V+ + G + I EL+L+             H ++        L          H+A    HLN+V+ LL+ G S   C   +G   +H+A     VD  R LL      +  + +   P+H+     +  ++ L LS+  +  L ++
Sbjct:  279 ASRRGNVIMVRLLLDRGA-QIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP--LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE----------REAHPNSAGKTGLTPL----------HVAVYHNHLNIVRLLLNKGASP-HCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNK 699          

HSP 3 Score: 118.627 bits (296), Expect = 2.759e-26
Identity = 102/461 (22.13%), Postives = 197/461 (42.73%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD----------------LEARNNFSQYLI-------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASI 1378
            +A   G+L + L+ L R   +I   N+N    +H +    +V +V  L+     +E    +    +H+A   G  D+V+ L+++G +++     G +P ++A +  HL ++KFL+ NG++                 +   N   YLI             H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +  +VN     G   +H+A   G++ ++  L       D+     + P+  A + G   I ++LLD+       I  + K  +  +  + +   +D  ++ + +   I+       +PL+V+   GH  + +L+LD              S+A  +     L          HIAC   H+ V++ LL  G S I+    SG   +H+A    H+ IV+ LL +    ++ + + + P+HM   +++  +
Sbjct:   51 RAARAGNLDKALDHL-RNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGA----PIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD----------KGAKSNARALNGFTPL----------HIACKKSHIRVMELLLKSGAS-IDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDV 485          

HSP 4 Score: 116.701 bits (291), Expect = 1.059e-25
Identity = 75/302 (24.83%), Postives = 131/302 (43.38%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     + +      + P+H      +    ELL+ N+    +   +   P+H+A KYG + I +LL+      +     G +P H+A    HL+I++ L++ G+     ARN ++   +H A +   ++ A+ L+       A + +   P+H+A + G  D+ +LL+ +  +V+  N     P+HLA   GHL I E L        +       P+  AC  G   +V+ LL +  N N         V  L +                     +PL+ + Q+GH DIV L+L
Sbjct:  517 EMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP--LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNA--------VTKLGF---------------------TPLHQAAQQGHTDIVTLLL 787          

HSP 5 Score: 112.849 bits (281), Expect = 1.431e-24
Identity = 64/238 (26.89%), Postives = 115/238 (48.32%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            I++  LL  +  +     +    P+H A+ ++++ IV LL++              P+H+A K  ++D  + L+  G   +   L G +P HLA + GH D++  L+S  +++   N  S   +H A +   + IA+ L+S++ ++ A       P+H+AC YG   +   L+Q   +VNA    G  P+H A   GH  I+  L K     + A+   + P+  A +LG  S+  +L
Sbjct:  582 IQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 819          

HSP 6 Score: 108.612 bits (270), Expect = 3.473e-23
Identity = 94/414 (22.71%), Postives = 177/414 (42.75%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD------ARLRNVRNLTNNCHSSAEDMIKC-------QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            + N  DI   N   +  +HL+ K G++ +V  L+     L      GN+  H+A   G  D+++ L+++G+++ +++      ++ A + N + + KFL+ N  N          P+ +A + G E++ + LI          SYG +       +H+A  N   R    L +   + D  +     P+  A      ++ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ I E++LD      A+ +N   L+    +S  D + C       Q+ IDD      T  H+A   GH  V + LLD G +      ++G   +HIAC  SH+ ++  LL      D + +    P+H+     +  I+   L +  S
Sbjct:   65 LRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLI----------SYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVN---FTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKN--GLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKG-AKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGAS 462          

HSP 7 Score: 103.99 bits (258), Expect = 8.627e-22
Identity = 92/458 (20.09%), Postives = 178/458 (38.86%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD-------GNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +V+  L     N+   ++  + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+ ++V  LIS G    V                      ND +       G +P H+A  + +L++ + L++ G+++          +H A R   + + + L+     ++A       P+H A   G   I  +L+     + A+   G  PIH+A    HL  +  L + +   D     ++ P+  A   G   + +LLLD    +N                   + ++ F           +PL+++C++ H+ ++EL+L                      K  + ID   E   T  H+A   GHL +V+ LL  G S      +  +  +H+A   +H D+  FL+      +   K  Q P+H    S +  ++ L L      D
Sbjct:  125 DVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSN------------------ARALNGF-----------TPLHIACKKSHIRVMELLL----------------------KSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASP-NVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPD 530          

HSP 8 Score: 103.99 bits (258), Expect = 9.392e-22
Identity = 56/226 (24.78%), Postives = 110/226 (48.67%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + I ELL+  +    +     + P+H+A  + +L+IV+LL++ G        +G +P H+A +   +D  + L+  G+   A++      +H A +    ++   L+S   N+   N     P+H+A + G  +I  +L+  +  V AE   G  P+H+AC  G++++++ L + + + ++       P+  A + G   IV LLL N    N+
Sbjct:  570 FTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQ 795          

HSP 9 Score: 78.1814 bits (191), Expect = 5.972e-14
Identity = 77/351 (21.94%), Postives = 150/351 (42.74%), Query Frame = 2
Query:  410 ACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNV----RNLTNNCH--SSAEDMIKCQSLIDDEKE-RFCT-------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            A   G+LD     + NG D+   N      +H + +   +++   L+  +  LE    K +  +HIA   G ED+   L+    +VN+++  G  P+++A    HL +++ L +   + + A      P+  A + G +++V  L+         +        H+       R     +    + ++ S     PL+++    +L++ +L+L+ R  NV    +N  +  H  S   ++I  + L+D   +   CT       H A  +GH+ + + LLD G + I+ +  +G   IH+A    H+D VR LL      D +   +  P+H+     +  +  L L +  
Sbjct:   52 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRL-----PALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLN-RGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHG-APIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGA 395          
BLAST of SMED30018624 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 154.066 bits (388), Expect = 3.428e-37
Identity = 100/407 (24.57%), Postives = 181/407 (44.47%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N + P+H A   S++ ++ELL+ +   I+A  +  + P+H+A   G+L IV+ L+  G   +V  +   +P H+A    H D+  FLI N + + AR    Q  +H A R    E+ + L+ N  + + P      P+H+    G      LL+       +    G  P+H+A   G ++I E L + +   +SA    + P+  A      +IV+LLL+   + + T    +  + H+   +++    R  +  G     +S     PL+++ Q GH D+V L+L  +         N H            + ++      H+A   GHLN+ + L+    S +  E   G   +H+AC + ++ +V+FLL      + + K    P+H      +  I+ L L
Sbjct:  366 NGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPL-HMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQA--------NVH------------LGNKSSLTPLHLASQEGHLNIAEVLVSHDAS-VAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 750          

HSP 2 Score: 142.895 bits (359), Expect = 9.538e-34
Identity = 109/445 (24.49%), Postives = 198/445 (44.49%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKR----IDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A   G++I V  LL R    I+   ++   P+H A+ N +V I E+L+ +   I+A+    + PIH+A +  +LD V+LL+     +    LD  +P H+A   GH  + K L+  G+   AR  N F+   +H AC+ + I + + L+ +  +++A       P+H+A   G   I   L+Q+    N  +     P+H+A    H  + + L + K   ++    +  P+  A + G   +V+LLL+N  + +      H  ++       T+     +D    H    K+  +PL+V+ + G + I EL+L+             H ++        L          H+A    HLN+V+ LL+ G S   C   +G   +H+A     VD  R LL      +  + +   P+H+     +  ++ L LS+  +  L ++
Sbjct:  242 ASRRGNVIMVRLLLDRGA-QIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP--LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE----------REAHPNSAGKTGLTPL----------HVAVYHNHLNIVRLLLNKGASP-HCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNK 662          

HSP 3 Score: 118.242 bits (295), Expect = 3.170e-26
Identity = 102/461 (22.13%), Postives = 197/461 (42.73%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD----------------LEARNNFSQYLI-------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASI 1378
            +A   G+L + L+ L R   +I   N+N    +H +    +V +V  L+     +E    +    +H+A   G  D+V+ L+++G +++     G +P ++A +  HL ++KFL+ NG++                 +   N   YLI             H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +  +VN     G   +H+A   G++ ++  L       D+     + P+  A + G   I ++LLD+       I  + K  +  +  + +   +D  ++ + +   I+       +PL+V+   GH  + +L+LD              S+A  +     L          HIAC   H+ V++ LL  G S I+    SG   +H+A    H+ IV+ LL +    ++ + + + P+HM   +++  +
Sbjct:   14 RAARAGNLDKALDHL-RNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGA----PIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD----------KGAKSNARALNGFTPL----------HIACKKSHIRVMELLLKSGAS-IDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDV 448          

HSP 4 Score: 116.316 bits (290), Expect = 1.217e-25
Identity = 75/302 (24.83%), Postives = 131/302 (43.38%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     + +      + P+H      +    ELL+ N+    +   +   P+H+A KYG + I +LL+      +     G +P H+A    HL+I++ L++ G+     ARN ++   +H A +   ++ A+ L+       A + +   P+H+A + G  D+ +LL+ +  +V+  N     P+HLA   GHL I E L        +       P+  AC  G   +V+ LL +  N N         V  L +                     +PL+ + Q+GH DIV L+L
Sbjct:  480 EMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP--LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNA--------VTKLGF---------------------TPLHQAAQQGHTDIVTLLL 750          

HSP 5 Score: 112.849 bits (281), Expect = 1.537e-24
Identity = 64/238 (26.89%), Postives = 115/238 (48.32%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            I++  LL  +  +     +    P+H A+ ++++ IV LL++              P+H+A K  ++D  + L+  G   +   L G +P HLA + GH D++  L+S  +++   N  S   +H A +   + IA+ L+S++ ++ A       P+H+AC YG   +   L+Q   +VNA    G  P+H A   GH  I+  L K     + A+   + P+  A +LG  S+  +L
Sbjct:  545 IQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 782          

HSP 6 Score: 108.227 bits (269), Expect = 3.859e-23
Identity = 94/414 (22.71%), Postives = 177/414 (42.75%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD------ARLRNVRNLTNNCHSSAEDMIKC-------QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            + N  DI   N   +  +HL+ K G++ +V  L+     L      GN+  H+A   G  D+++ L+++G+++ +++      ++ A + N + + KFL+ N  N          P+ +A + G E++ + LI          SYG +       +H+A  N   R    L +   + D  +     P+  A      ++ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ I E++LD      A+ +N   L+    +S  D + C       Q+ IDD      T  H+A   GH  V + LLD G +      ++G   +HIAC  SH+ ++  LL      D + +    P+H+     +  I+   L +  S
Sbjct:   28 LRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLI----------SYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVN---FTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKN--GLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKG-AKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGAS 425          

HSP 7 Score: 103.605 bits (257), Expect = 1.000e-21
Identity = 56/226 (24.78%), Postives = 110/226 (48.67%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + I ELL+  +    +     + P+H+A  + +L+IV+LL++ G        +G +P H+A +   +D  + L+  G+   A++      +H A +    ++   L+S   N+   N     P+H+A + G  +I  +L+  +  V AE   G  P+H+AC  G++++++ L + + + ++       P+  A + G   IV LLL N    N+
Sbjct:  533 FTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQ 758          

HSP 8 Score: 103.605 bits (257), Expect = 1.108e-21
Identity = 92/458 (20.09%), Postives = 178/458 (38.86%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD-------GNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +V+  L     N+   ++  + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+ ++V  LIS G    V                      ND +       G +P H+A  + +L++ + L++ G+++          +H A R   + + + L+     ++A       P+H A   G   I  +L+     + A+   G  PIH+A    HL  +  L + +   D     ++ P+  A   G   + +LLLD    +N                   + ++ F           +PL+++C++ H+ ++EL+L                      K  + ID   E   T  H+A   GHL +V+ LL  G S      +  +  +H+A   +H D+  FL+      +   K  Q P+H    S +  ++ L L      D
Sbjct:   88 DVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSN------------------ARALNGF-----------TPLHIACKKSHIRVMELLL----------------------KSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASP-NVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPD 493          

HSP 9 Score: 78.1814 bits (191), Expect = 6.645e-14
Identity = 77/351 (21.94%), Postives = 150/351 (42.74%), Query Frame = 2
Query:  410 ACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNV----RNLTNNCH--SSAEDMIKCQSLIDDEKE-RFCT-------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            A   G+LD     + NG D+   N      +H + +   +++   L+  +  LE    K +  +HIA   G ED+   L+    +VN+++  G  P+++A    HL +++ L +   + + A      P+  A + G +++V  L+         +        H+       R     +    + ++ S     PL+++    +L++ +L+L+ R  NV    +N  +  H  S   ++I  + L+D   +   CT       H A  +GH+ + + LLD G + I+ +  +G   IH+A    H+D VR LL      D +   +  P+H+     +  +  L L +  
Sbjct:   15 AARAGNLDKALDHLRNGVDINTCNQNGLNALHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRL-----PALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLN-RGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHG-APIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGA 358          
BLAST of SMED30018624 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 154.066 bits (388), Expect = 3.531e-37
Identity = 100/407 (24.57%), Postives = 181/407 (44.47%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N + P+H A   S++ ++ELL+ +   I+A  +  + P+H+A   G+L IV+ L+  G   +V  +   +P H+A    H D+  FLI N + + AR    Q  +H A R    E+ + L+ N  + + P      P+H+    G      LL+       +    G  P+H+A   G ++I E L + +   +SA    + P+  A      +IV+LLL+   + + T    +  + H+   +++    R  +  G     +S     PL+++ Q GH D+V L+L  +         N H            + ++      H+A   GHLN+ + L+    S +  E   G   +H+AC + ++ +V+FLL      + + K    P+H      +  I+ L L
Sbjct:  328 NGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPL-HMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQA--------NVH------------LGNKSSLTPLHLASQEGHLNIAEVLVSHDAS-VAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 712          

HSP 2 Score: 142.895 bits (359), Expect = 9.663e-34
Identity = 109/445 (24.49%), Postives = 198/445 (44.49%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKR----IDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A   G++I V  LL R    I+   ++   P+H A+ N +V I E+L+ +   I+A+    + PIH+A +  +LD V+LL+     +    LD  +P H+A   GH  + K L+  G+   AR  N F+   +H AC+ + I + + L+ +  +++A       P+H+A   G   I   L+Q+    N  +     P+H+A    H  + + L + K   ++    +  P+  A + G   +V+LLL+N  + +      H  ++       T+     +D    H    K+  +PL+V+ + G + I EL+L+             H ++        L          H+A    HLN+V+ LL+ G S   C   +G   +H+A     VD  R LL      +  + +   P+H+     +  ++ L LS+  +  L ++
Sbjct:  204 ASRRGNVIMVRLLLDRGA-QIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTP--LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE----------REAHPNSAGKTGLTPL----------HVAVYHNHLNIVRLLLNKGASP-HCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNK 624          

HSP 3 Score: 119.398 bits (298), Expect = 1.558e-26
Identity = 92/424 (21.70%), Postives = 191/424 (45.05%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASI 1378
            ++++  L  K   +E   +     +H A       +V  L+++  ++ +++ +   P+++A +  +L +V+ L+ NG + ++   DG +P  +A + GH +++ +LIS G   + R       +H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +  +VN     G   +H+A   G++ ++  L       D+     + P+  A + G   I ++LLD+       I  + K  +  +  + +   +D  ++ + +   I+       +PL+V+   GH  + +L+LD              S+A  +     L          HIAC   H+ V++ LL  G S I+    SG   +H+A    H+ IV+ LL +    ++ + + + P+HM   +++  +
Sbjct:   16 VKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVR----LPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGA----PIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD----------KGAKSNARALNGFTPL----------HIACKKSHIRVMELLLKSGAS-IDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDV 410          

HSP 4 Score: 116.701 bits (291), Expect = 1.153e-25
Identity = 75/302 (24.83%), Postives = 131/302 (43.38%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL     + +      + P+H      +    ELL+ N+    +   +   P+H+A KYG + I +LL+      +     G +P H+A    HL+I++ L++ G+     ARN ++   +H A +   ++ A+ L+       A + +   P+H+A + G  D+ +LL+ +  +V+  N     P+HLA   GHL I E L        +       P+  AC  G   +V+ LL +  N N         V  L +                     +PL+ + Q+GH DIV L+L
Sbjct:  442 EMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTP--LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNA--------VTKLGF---------------------TPLHQAAQQGHTDIVTLLL 712          

HSP 5 Score: 112.849 bits (281), Expect = 1.585e-24
Identity = 64/238 (26.89%), Postives = 115/238 (48.32%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            I++  LL  +  +     +    P+H A+ ++++ IV LL++              P+H+A K  ++D  + L+  G   +   L G +P HLA + GH D++  L+S  +++   N  S   +H A +   + IA+ L+S++ ++ A       P+H+AC YG   +   L+Q   +VNA    G  P+H A   GH  I+  L K     + A+   + P+  A +LG  S+  +L
Sbjct:  507 IQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVL 744          

HSP 6 Score: 104.375 bits (259), Expect = 7.285e-22
Identity = 90/397 (22.67%), Postives = 171/397 (43.07%), Query Frame = 2
Query:  302 IHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD------ARLRNVRNLTNNCHSSAEDMIKC-------QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +HL+ K G++ +V  L+     L      GN+  H+A   G  D+++ L+++G+++ +++      ++ A + N + + KFL+ N  N          P+ +A + G E++ + LI          SYG +       +H+A  N   R    L +   + D  +     P+  A      ++ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ I E++LD      A+ +N   L+    +S  D + C       Q+ IDD      T  H+A   GH  V + LLD G +      ++G   +HIAC  SH+ ++  LL      D + +    P+H+     +  I+   L +  S
Sbjct:    7 LHLSSKEGHVKMVTELLHKDIVLETTTKKGNTALHIAALAGQEDVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLI----------SYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVN---FTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKN--GLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKG-AKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGAS 387          

HSP 7 Score: 103.99 bits (258), Expect = 9.481e-22
Identity = 56/226 (24.78%), Postives = 110/226 (48.67%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + I ELL+  +    +     + P+H+A  + +L+IV+LL++ G        +G +P H+A +   +D  + L+  G+   A++      +H A +    ++   L+S   N+   N     P+H+A + G  +I  +L+  +  V AE   G  P+H+AC  G++++++ L + + + ++       P+  A + G   IV LLL N    N+
Sbjct:  495 FTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQ 720          

HSP 8 Score: 103.605 bits (257), Expect = 1.015e-21
Identity = 92/458 (20.09%), Postives = 178/458 (38.86%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD-------GNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +V+  L     N+   ++  + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+ ++V  LIS G    V                      ND +       G +P H+A  + +L++ + L++ G+++          +H A R   + + + L+     ++A       P+H A   G   I  +L+     + A+   G  PIH+A    HL  +  L + +   D     ++ P+  A   G   + +LLLD    +N                   + ++ F           +PL+++C++ H+ ++EL+L                      K  + ID   E   T  H+A   GHL +V+ LL  G S      +  +  +H+A   +H D+  FL+      +   K  Q P+H    S +  ++ L L      D
Sbjct:   50 DVVRELVNHGANVNSQSQKGFTPLYMAAQENHLPVVKFLLENGANQNLATEDGFTPLAVALQQGHENVVAYLISYGMKGKVRLPALHIAARNDDTRTAAVLLQNDPNADVLSKTGFTPLHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSN------------------ARALNGF-----------TPLHIACKKSHIRVMELLL----------------------KSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASP-NVFSVKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPD 455          
BLAST of SMED30018624 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 150.984 bits (380), Expect = 1.420e-36
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  376 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 781          

HSP 2 Score: 135.576 bits (340), Expect = 9.098e-32
Identity = 95/418 (22.73%), Postives = 177/418 (42.34%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+   + L AR       +H A + + +E  K L+     ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  222 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 617          

HSP 3 Score: 124.79 bits (312), Expect = 2.088e-28
Identity = 114/475 (24.00%), Postives = 203/475 (42.74%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L      D +
Sbjct:   62 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAA 522          

HSP 4 Score: 121.709 bits (304), Expect = 1.915e-27
Identity = 101/448 (22.54%), Postives = 185/448 (41.29%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+     L     +G SP H+A +  H++ +K L+   + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     + + K+   P+H+     +  ++ L L +  +  +S
Sbjct:  242 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 687          

HSP 5 Score: 121.324 bits (303), Expect = 2.196e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:  100 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQH-MAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 586          

HSP 6 Score: 100.523 bits (249), Expect = 5.552e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  507 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 777          

HSP 7 Score: 99.7525 bits (247), Expect = 1.079e-20
Identity = 58/238 (24.37%), Postives = 106/238 (44.54%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + +     G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  572 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 809          

HSP 8 Score: 93.9745 bits (232), Expect = 6.603e-19
Identity = 84/413 (20.34%), Postives = 160/413 (38.74%), Query Frame = 2
Query:   83 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            +  E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + ++     + P+  A + G   +V LLLD   N    I+M  K  +  L  + +  +++   +   H  + +       +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  458 IRGETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGAN----IHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 809          

HSP 9 Score: 86.6557 bits (213), Expect = 1.291e-16
Identity = 71/360 (19.72%), Postives = 145/360 (40.28%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS                                 ++++   K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM
Sbjct:   34 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGS---------------------------------SVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHM 334          
BLAST of SMED30018624 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 150.984 bits (380), Expect = 1.446e-36
Identity = 105/428 (24.53%), Postives = 189/428 (44.16%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L    
Sbjct:  359 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGA 764          

HSP 2 Score: 135.191 bits (339), Expect = 1.031e-31
Identity = 95/418 (22.73%), Postives = 177/418 (42.34%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+   + L AR       +H A + + +E  K L+     ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  205 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 600          

HSP 3 Score: 124.79 bits (312), Expect = 1.984e-28
Identity = 114/475 (24.00%), Postives = 203/475 (42.74%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N    + +       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L      D +
Sbjct:   45 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAA 505          

HSP 4 Score: 121.324 bits (303), Expect = 2.381e-27
Identity = 101/448 (22.54%), Postives = 185/448 (41.29%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAE----DMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+     L     +G SP H+A +  H++ +K L+   + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+    D+ K     ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     + + K+   P+H+     +  ++ L L +  +  +S
Sbjct:  225 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 670          

HSP 5 Score: 121.324 bits (303), Expect = 2.594e-27
Identity = 108/495 (21.82%), Postives = 204/495 (41.21%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSV----------------------NDLD---------------GNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G   EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +     +    P+ +A + G+   V +L+ N     V                      ND +               G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:   83 ASLAGQ-AEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQH-MAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 569          

HSP 6 Score: 100.908 bits (250), Expect = 5.178e-21
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  490 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 760          

HSP 7 Score: 99.7525 bits (247), Expect = 9.330e-21
Identity = 58/238 (24.37%), Postives = 106/238 (44.54%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + +     G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  555 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 8 Score: 93.2041 bits (230), Expect = 1.024e-18
Identity = 84/413 (20.34%), Postives = 160/413 (38.74%), Query Frame = 2
Query:   83 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISN--GSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL 1291
            +  E  L  A   G  +EV+  L R    ++        P+H A       IV+LL+ +    +A       P+H++ + G +D+  +L+  G   S+    G +P H+A ++G LD+ K L+     +D   +N  +                                   P+H+A  Y  + +  LL+++    +A    G  P+H+A     ++I   L     + ++     + P+  A + G   +V LLLD   N    I+M  K  +  L  + +  +++   +   H  + +       +PL V+C  G++ +V  +L  +  NV   T N ++                     H A   GH +++  LL  G +       +G+  + IA    ++ +V  L
Sbjct:  441 IRGETALHMAARAGQ-VEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLT-----------------------------------PLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGAN----IHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLK-QGANVNAKTKNGYTP-------------------LHQAAQQGHTHIINVLLQHG-AKPNATTANGNTALAIAKRLGYISVVDTL 792          

HSP 9 Score: 86.2705 bits (212), Expect = 1.482e-16
Identity = 71/360 (19.72%), Postives = 145/360 (40.28%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS                                 ++++   K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM
Sbjct:   17 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGS---------------------------------SVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHM 317          
BLAST of SMED30018624 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 146.362 bits (368), Expect = 5.260e-35
Identity = 91/412 (22.09%), Postives = 185/412 (44.90%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            N + P+H A   + + ++ELL+ +   I+A  +  + PIH+A   G+++IV  L+ +G   +  ++ G +  H+A   G  +++++L+ +G+ +EA+    Q  +H + R  + +I + L+    +  A       P+H+A   G ED+ + L+     ++     G  P+H+A   G L +   L +     D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  + ++++ Q GH+D+V L+L +R  NV NL+N    +                    H+A     +NV + L++ G + ++ +   G   +H+ C + ++ IV FLL  +   +   K     +H      +  I+ + L  N S
Sbjct:  382 NGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPL-HIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL-SRNANV-NLSNKSGLTP------------------LHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771          

HSP 2 Score: 137.887 bits (346), Expect = 2.143e-32
Identity = 96/435 (22.07%), Postives = 188/435 (43.22%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLI--SNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            D  +   LL +  TN +  +++ + P+H A    N+ +  LL+  +   D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+   + + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +L+ +    NA+   G  P+H+AC    +R++E L K      +     + PI  A  +G  +IV  L+ +  + N T  +  +   H+        + R+ +  G       K+ ++PL++S + G  DIV+ +L       +  + N  +++                   H+A   GH +V  +LLD G S +      G   +H+A  +  +++   LL ++   D   K    P+H+        +  L L +  S
Sbjct:  196 DDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARND--ITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPN-TTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQ------QGASPNAATTS--------------GYTPLHLAAREGHEDVAAFLLDHGAS-LSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGAS 606          

HSP 3 Score: 130.183 bits (326), Expect = 5.121e-30
Identity = 103/428 (24.07%), Postives = 193/428 (45.09%), Query Frame = 2
Query:  182 NENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTI-----------YMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            N+N    +H A    +V +V  L+  + +++A   +    +H+A   G  ++V++L++NG +++    +G +P ++A +  HL++++FL+ NG+   L   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD     +                 H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L   +  V ++TN+     H +A     K   ++ D+K          F   HIAC    + V++ LL  G S I+    SG   IH+A    HV+IV  L+      +  N R +  +HM   S  A ++
Sbjct:   54 NQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEML-------LDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNV-PVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGAS-IQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVV 465          

HSP 4 Score: 107.071 bits (266), Expect = 7.183e-23
Identity = 62/238 (26.05%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV +LL +K+ + +   ++   P+H A    N  +  LL+       A       P+H+A K   +DI   L+  G D +     G +  HLA + GH+D++  L+S  +++   N      +H A + +R+ +A+ L++   +++A       P+H+ C YG   I + L+Q +  VNA+   G   +H A   GH  II  L +     +         +  A +LG  S+V  L
Sbjct:  561 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 103.219 bits (256), Expect = 1.109e-21
Identity = 53/226 (23.45%), Postives = 102/226 (45.13%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + +  LL+      +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   +DI   L+  G+D  A        +H A +   +++   L+S N N+   N     P+H+A +    ++  +L+ Q   V+A+   G  P+H+ C  G+++I+  L +     ++        +  A + G   I+ +LL NN + N+
Sbjct:  549 FTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNE 774          

HSP 6 Score: 99.7525 bits (247), Expect = 1.348e-20
Identity = 78/348 (22.41%), Postives = 140/348 (40.23%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   GHL+     I NG D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++  +L+    +VNA++  G  P+++A    HL ++  L         A      P+  A + G   +V LLL+N+      +        H+   K   +     +    + ++ES     PL+++   G++++  L+L+              ++A D      +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR+       K    P+HM     + + + L L  N   D
Sbjct:   23 DANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RAAAVDFTARNDIT-------PLHVASKRGNANMVKLLLDRG-AKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVD 344          
BLAST of SMED30018624 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 146.362 bits (368), Expect = 6.044e-35
Identity = 91/412 (22.09%), Postives = 185/412 (44.90%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            N + P+H A   + + ++ELL+ +   I+A  +  + PIH+A   G+++IV  L+ +G   +  ++ G +  H+A   G  +++++L+ +G+ +EA+    Q  +H + R  + +I + L+    +  A       P+H+A   G ED+ + L+     ++     G  P+H+A   G L +   L +     D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  + ++++ Q GH+D+V L+L +R  NV NL+N    +                    H+A     +NV + L++ G + ++ +   G   +H+ C + ++ IV FLL  +   +   K     +H      +  I+ + L  N S
Sbjct:  393 NGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPL-HIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL-SRNANV-NLSNKSGLTP------------------LHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 782          

HSP 2 Score: 137.887 bits (346), Expect = 2.200e-32
Identity = 96/435 (22.07%), Postives = 188/435 (43.22%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLI--SNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            D  +   LL +  TN +  +++ + P+H A    N+ +  LL+  +   D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+   + + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +L+ +    NA+   G  P+H+AC    +R++E L K      +     + PI  A  +G  +IV  L+ +  + N T  +  +   H+        + R+ +  G       K+ ++PL++S + G  DIV+ +L       +  + N  +++                   H+A   GH +V  +LLD G S +      G   +H+A  +  +++   LL ++   D   K    P+H+        +  L L +  S
Sbjct:  207 DDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARND--ITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPN-TTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQ------QGASPNAATTS--------------GYTPLHLAAREGHEDVAAFLLDHGAS-LSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGAS 617          

HSP 3 Score: 129.798 bits (325), Expect = 7.649e-30
Identity = 105/428 (24.53%), Postives = 195/428 (45.56%), Query Frame = 2
Query:  182 NENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KT----------IYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            N+N    +H A    +V +V  L+  + +++A   +    +H+A   G  ++V++L++NG +++    +G +P ++A +  HL++++FL+ NG+   L   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD     + KT              H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L   +  V ++TN+     H +A     K   ++ D+K          F   HIAC    + V++ LL  G S I+    SG   IH+A    HV+IV  L+      +  N R +  +HM   S  A ++
Sbjct:   65 NQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEML-------LDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNV-PVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGAS-IQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVV 476          

HSP 4 Score: 107.071 bits (266), Expect = 7.544e-23
Identity = 62/238 (26.05%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV +LL +K+ + +   ++   P+H A    N  +  LL+       A       P+H+A K   +DI   L+  G D +     G +  HLA + GH+D++  L+S  +++   N      +H A + +R+ +A+ L++   +++A       P+H+ C YG   I + L+Q +  VNA+   G   +H A   GH  II  L +     +         +  A +LG  S+V  L
Sbjct:  572 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 809          

HSP 5 Score: 102.834 bits (255), Expect = 1.289e-21
Identity = 53/226 (23.45%), Postives = 102/226 (45.13%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + +  LL+      +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   +DI   L+  G+D  A        +H A +   +++   L+S N N+   N     P+H+A +    ++  +L+ Q   V+A+   G  P+H+ C  G+++I+  L +     ++        +  A + G   I+ +LL NN + N+
Sbjct:  560 FTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNE 785          

HSP 6 Score: 99.3673 bits (246), Expect = 1.795e-20
Identity = 78/348 (22.41%), Postives = 140/348 (40.23%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   GHL+     I NG D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++  +L+    +VNA++  G  P+++A    HL ++  L         A      P+  A + G   +V LLL+N+      +        H+   K   +     +    + ++ES     PL+++   G++++  L+L+              ++A D      +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR+       K    P+HM     + + + L L  N   D
Sbjct:   34 DANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RAAAVDFTARNDIT-------PLHVASKRGNANMVKLLLDRG-AKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVD 355          
BLAST of SMED30018624 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 146.362 bits (368), Expect = 6.187e-35
Identity = 91/412 (22.09%), Postives = 185/412 (44.90%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            N + P+H A   + + ++ELL+ +   I+A  +  + PIH+A   G+++IV  L+ +G   +  ++ G +  H+A   G  +++++L+ +G+ +EA+    Q  +H + R  + +I + L+    +  A       P+H+A   G ED+ + L+     ++     G  P+H+A   G L +   L +     D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  + ++++ Q GH+D+V L+L +R  NV NL+N    +                    H+A     +NV + L++ G + ++ +   G   +H+ C + ++ IV FLL  +   +   K     +H      +  I+ + L  N S
Sbjct:  382 NGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPL-HIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL-SRNANV-NLSNKSGLTP------------------LHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771          

HSP 2 Score: 137.887 bits (346), Expect = 2.409e-32
Identity = 96/435 (22.07%), Postives = 188/435 (43.22%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLI--SNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            D  +   LL +  TN +  +++ + P+H A    N+ +  LL+  +   D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+   + + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +L+ +    NA+   G  P+H+AC    +R++E L K      +     + PI  A  +G  +IV  L+ +  + N T  +  +   H+        + R+ +  G       K+ ++PL++S + G  DIV+ +L       +  + N  +++                   H+A   GH +V  +LLD G S +      G   +H+A  +  +++   LL ++   D   K    P+H+        +  L L +  S
Sbjct:  196 DDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARND--ITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPN-TTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQ------QGASPNAATTS--------------GYTPLHLAAREGHEDVAAFLLDHGAS-LSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGAS 606          

HSP 3 Score: 129.798 bits (325), Expect = 6.932e-30
Identity = 105/428 (24.53%), Postives = 195/428 (45.56%), Query Frame = 2
Query:  182 NENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KT----------IYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            N+N    +H A    +V +V  L+  + +++A   +    +H+A   G  ++V++L++NG +++    +G +P ++A +  HL++++FL+ NG+   L   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD     + KT              H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L   +  V ++TN+     H +A     K   ++ D+K          F   HIAC    + V++ LL  G S I+    SG   IH+A    HV+IV  L+      +  N R +  +HM   S  A ++
Sbjct:   54 NQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEML-------LDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNV-PVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGAS-IQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVV 465          

HSP 4 Score: 106.686 bits (265), Expect = 8.800e-23
Identity = 62/238 (26.05%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV +LL +K+ + +   ++   P+H A    N  +  LL+       A       P+H+A K   +DI   L+  G D +     G +  HLA + GH+D++  L+S  +++   N      +H A + +R+ +A+ L++   +++A       P+H+ C YG   I + L+Q +  VNA+   G   +H A   GH  II  L +     +         +  A +LG  S+V  L
Sbjct:  561 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 102.834 bits (255), Expect = 1.335e-21
Identity = 53/226 (23.45%), Postives = 102/226 (45.13%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + +  LL+      +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   +DI   L+  G+D  A        +H A +   +++   L+S N N+   N     P+H+A +    ++  +L+ Q   V+A+   G  P+H+ C  G+++I+  L +     ++        +  A + G   I+ +LL NN + N+
Sbjct:  549 FTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNE 774          

HSP 6 Score: 99.7525 bits (247), Expect = 1.345e-20
Identity = 78/348 (22.41%), Postives = 140/348 (40.23%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   GHL+     I NG D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++  +L+    +VNA++  G  P+++A    HL ++  L         A      P+  A + G   +V LLL+N+      +        H+   K   +     +    + ++ES     PL+++   G++++  L+L+              ++A D      +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR+       K    P+HM     + + + L L  N   D
Sbjct:   23 DANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RAAAVDFTARNDIT-------PLHVASKRGNANMVKLLLDRG-AKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVD 344          
BLAST of SMED30018624 vs. UniProt/SwissProt
Match: sp|G5E8K5|ANK3_MOUSE (Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 4.953e-34
Identity = 91/412 (22.09%), Postives = 185/412 (44.90%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            N + P+H A   + + ++ELL+ +   I+A  +  + PIH+A   G+++IV  L+ +G   +  ++ G +  H+A   G  +++++L+ +G+ +EA+    Q  +H + R  + +I + L+    +  A       P+H+A   G ED+ + L+     ++     G  P+H+A   G L +   L +     D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  + ++++ Q GH+D+V L+L +R  NV NL+N    +                    H+A     +NV + L++ G + ++ +   G   +H+ C + ++ IV FLL  +   +   K     +H      +  I+ + L  N S
Sbjct:  382 NGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPL-HIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL-SRNANV-NLSNKSGLTP------------------LHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771          

HSP 2 Score: 137.887 bits (346), Expect = 1.634e-31
Identity = 96/435 (22.07%), Postives = 188/435 (43.22%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLI--SNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            D  +   LL +  TN +  +++ + P+H A    N+ +  LL+  +   D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+   + + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +L+ +    NA+   G  P+H+AC    +R++E L K      +     + PI  A  +G  +IV  L+ +  + N T  +  +   H+        + R+ +  G       K+ ++PL++S + G  DIV+ +L       +  + N  +++                   H+A   GH +V  +LLD G S +      G   +H+A  +  +++   LL ++   D   K    P+H+        +  L L +  S
Sbjct:  196 DDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARND--ITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPN-TTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQ------QGASPNAATTS--------------GYTPLHLAAREGHEDVAAFLLDHGAS-LSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGAS 606          

HSP 3 Score: 129.798 bits (325), Expect = 5.852e-29
Identity = 105/428 (24.53%), Postives = 195/428 (45.56%), Query Frame = 2
Query:  182 NENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-KT----------IYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            N+N    +H A    +V +V  L+  + +++A   +    +H+A   G  ++V++L++NG +++    +G +P ++A +  HL++++FL+ NG+   L   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD     + KT              H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L   +  V ++TN+     H +A     K   ++ D+K          F   HIAC    + V++ LL  G S I+    SG   IH+A    HV+IV  L+      +  N R +  +HM   S  A ++
Sbjct:   54 NQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEML-------LDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNV-PVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGAS-IQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVV 465          

HSP 4 Score: 106.686 bits (265), Expect = 6.427e-22
Identity = 62/238 (26.05%), Postives = 110/238 (46.22%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV +LL +K+ + +   ++   P+H A    N  +  LL+       A       P+H+A K   +DI   L+  G D +     G +  HLA + GH+D++  L+S  +++   N      +H A + +R+ +A+ L++   +++A       P+H+ C YG   I + L+Q +  VNA+   G   +H A   GH  II  L +     +         +  A +LG  S+V  L
Sbjct:  561 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 102.834 bits (255), Expect = 1.051e-20
Identity = 53/226 (23.45%), Postives = 102/226 (45.13%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + +  LL+      +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   +DI   L+  G+D  A        +H A +   +++   L+S N N+   N     P+H+A +    ++  +L+ Q   V+A+   G  P+H+ C  G+++I+  L +     ++        +  A + G   I+ +LL NN + N+
Sbjct:  549 FTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNE 774          

HSP 6 Score: 99.3673 bits (246), Expect = 1.275e-19
Identity = 78/348 (22.41%), Postives = 140/348 (40.23%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   GHL+     I NG D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++  +L+    +VNA++  G  P+++A    HL ++  L         A      P+  A + G   +V LLL+N+      +        H+   K   +     +    + ++ES     PL+++   G++++  L+L+              ++A D      +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR+       K    P+HM     + + + L L  N   D
Sbjct:   23 DANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RAAAVDFTARNDIT-------PLHVASKRGNANMVKLLLDRG-AKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVD 344          
BLAST of SMED30018624 vs. UniProt/SwissProt
Match: sp|Q01484|ANK2_HUMAN (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4)

HSP 1 Score: 141.739 bits (356), Expect = 1.368e-32
Identity = 105/424 (24.76%), Postives = 188/424 (44.34%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEI-----ESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G    I      +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L
Sbjct:  380 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 2 Score: 129.798 bits (325), Expect = 7.085e-29
Identity = 96/418 (22.97%), Postives = 179/418 (42.82%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  226 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 621          

HSP 3 Score: 116.701 bits (291), Expect = 7.511e-25
Identity = 101/448 (22.54%), Postives = 188/448 (41.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+  G  L     +G SP H+A +  H++ +K L+ + + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+         +++ ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     +I+ K+   P+H+     +  ++ L L +  +  +S
Sbjct:  246 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 691          

HSP 4 Score: 114.005 bits (284), Expect = 4.695e-24
Identity = 114/466 (24.46%), Postives = 202/466 (43.35%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLT----------NNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N +  + R       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L
Sbjct:   66 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVR----LPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL 517          

HSP 5 Score: 110.538 bits (275), Expect = 4.877e-23
Identity = 107/487 (21.97%), Postives = 202/487 (41.48%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCD-------------------------IEAENDQK----MRPIHLACKYGNLDIVQLLISNGCDLSVNDL--------DGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +                         I  END K    +  +H+A +  +     LL+ N  +  V            G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:  111 EVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 590          

HSP 6 Score: 94.7449 bits (234), Expect = 3.326e-18
Identity = 58/238 (24.37%), Postives = 107/238 (44.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + ++    G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  576 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 7 Score: 93.2041 bits (230), Expect = 9.259e-18
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  511 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 781          

HSP 8 Score: 79.337 bits (194), Expect = 1.642e-13
Identity = 81/391 (20.72%), Postives = 163/391 (41.69%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILD---ARLRNVRNLTNNCHSSAE----DMIKCQSLID-----DEKER---FCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSR 1399
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS +++                                    K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+   A     RN     H +++    +M+K   L+D     D K R      H A  SGH  VV+ LL+ G + +     +G   +H+A    HV+ V+ LL      D +   Y   +H+     +  +  L L +
Sbjct:   38 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATK---------------------------------KGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKL--LLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERG-APLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDK 387          
BLAST of SMED30018624 vs. UniProt/SwissProt
Match: sp|Q8C8R3|ANK2_MOUSE (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 2.941e-32
Identity = 105/424 (24.76%), Postives = 189/424 (44.58%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            V  LL  K  N      N + P+H A   + + ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V ++ G +  H+A   G +++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  D+ S+L++     +     G  P+H+A   G L + + L + +   DSA    + P+  A     + +  LLL+   + + T    +  + H+   K   +I    ++ G       K+  +PL+++ Q GH D+V L+LD           N H S +  +               H+A     +NV   L   G +D +     G   + +AC + +V +V FLL +    +   K    P+H      +  I+ + L
Sbjct:  380 VTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPL-HIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDK--------GANIHMSTKSGLT------------SLHLAAQEDKVNVADILTKHG-ADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 2 Score: 125.561 bits (314), Expect = 1.273e-27
Identity = 95/418 (22.73%), Postives = 177/418 (42.34%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +    E+ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +++ L+   + L AR       +H A + + +E  K L+     ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + T  +  +   H+        + R  +  G       +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G ++V   LL+ G +        G   +H+A  +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  226 VNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVT-NIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQVDVASVLLEAGAAH-SLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGAS 621          

HSP 3 Score: 113.62 bits (283), Expect = 5.504e-24
Identity = 114/466 (24.46%), Postives = 202/466 (43.35%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPN-YKYDR-------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLT----------NNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N L  A   G +  V  LL R ++ ++   +     +H A       +V++L+    +I A++     P+++A +  ++D+V+ L+ NG + S    DG +P  +A + GH   +  L+ N +  + R       +H A R +  + A  L+ N+ N +  +    +R       P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  +++ L       D+     + P+  A + G   +V+LLL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A     V++VR LL      D   +  Q P+H+        I+ L L
Sbjct:   66 NALHLAAKEGHVGLVQELLGRGSS-VDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVR----LPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL 517          

HSP 4 Score: 111.694 bits (278), Expect = 2.555e-23
Identity = 107/487 (21.97%), Postives = 202/487 (41.48%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCD-------------------------IEAENDQK----MRPIHLACKYGNLDIVQLLISNGCDLSVNDL--------DGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELIL----DARLRNVRNLTNNCHSSAE----DMIKC----QSLIDDE--KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            EV+ +L ++  NI   ++N + P++ A   +++ +V+ L+ N  +                         I  END K    +  +H+A +  +     LL+ N  +  V            G +P H+A  +G++++   L++ G+  D  ARN  +   +H A +     + K L+     ++A       P+H A   G + +  LL+++   + A    G  P+H+A    H+  ++HL + K   D      +  +  A   G   + +LLLD   N N            L  + K  RI   ++ + +   I+       +P++V+   GHL+IV L+L       + N+R  T   H +A     ++++C     +L+D    +E+   HIA   G   +VQ LL    +  +    +G   +HI+     VD+   LL+    + +  K+   P+H+        +  L L R  + D
Sbjct:  111 EVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITP--LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARAL---NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETA-LHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAAD 590          

HSP 5 Score: 110.538 bits (275), Expect = 5.435e-23
Identity = 99/448 (22.10%), Postives = 185/448 (41.29%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE----SPLYVSCQRGHLDIVELILDA----RLRNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + V  LL  +   ++    N   P+H A    N  +V+LL+     I+A+    + P+H A + G+  +V+LL+     L     +G SP H+A +  H++ +K L+   + ++         +H A       + K L+    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GHL I+  L +     D  N      +  A + G   +V+ LL N    +     E   ++      KT+ +     H+ H         +PL++S + G +D+  ++L+A     L   +  T   H +A+         +++ ++  D   +   T  H+A    +  V   LL+ G S       +G   +HIA   + + I   LL+     + + K+   P+H+     +  ++ L L +  +  +S
Sbjct:  246 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFT-PLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASP-HATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMS 691          

HSP 6 Score: 93.5893 bits (231), Expect = 8.281e-18
Identity = 67/300 (22.33%), Postives = 125/300 (41.67%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL +   + +    N Y P+H +     V +  +L+           +   P+H+A KYG+LD+ +LL+           +G +P H+A  + +  +   L+  G+   A        +H A + N+++IA  L++          +   P+H+A + G  D+ +LL+ +  +++     G   +HLA     + + + L K   D D+       P+  AC  G   +V  LL    N N                 KTK         G+     +PL+ + Q+GH  I+ ++L
Sbjct:  511 EIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVN----------------AKTKN--------GY-----TPLHQAAQQGHTHIINVLL 781          

HSP 7 Score: 93.5893 bits (231), Expect = 9.012e-18
Identity = 58/238 (24.37%), Postives = 106/238 (44.54%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+       A       P+H+A K   + I   L++ G + +     G +P HLA + GH D++  L+  G+++          +H A + +++ +A  L  +  + +A       P+ +AC YG   + + L++Q  +VNA+   G  P+H A   GH  II  L +     ++        +  A +LG  S+V  L
Sbjct:  576 LDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          

HSP 8 Score: 77.0258 bits (188), Expect = 7.829e-13
Identity = 73/381 (19.16%), Postives = 148/381 (38.85%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A + GNLD V   +  G D++  + +G +  HLA + GH+ +++ L+  GS +++                                    K +  +HIA   G  ++  +L+++  ++NA++  G  P+++A    H+ ++++L +   +  +A      P+  A + G    V +LL+N+      +        H+   K   +     +   H+ +++S             PL+++   G++++  L+L+               +A D      +          H+A   G+ N+V+ LLD G   I+ +   G   +H A    H  +V  LL+R        K    P+HM     +   +   L      D
Sbjct:   38 AARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATK---------------------------------KGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLN-------------RGAAVDFTARNGITP-------LHVASKRGNTNMVKLLLDRG-GQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVD 359          
BLAST of SMED30018624 vs. UniProt/SwissProt
Match: sp|P16157|ANK1_HUMAN (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 137.117 bits (344), Expect = 2.701e-31
Identity = 96/436 (22.02%), Postives = 176/436 (40.37%), Query Frame = 2
Query:  113 CLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
               GD ++ + LL +    I++I  +   P+H A    +  + ++L+       +       P+H+ACK  ++ +++LL+  G  +      G +P H+A   GHL I+K L+  G+     N   +  +H A R    E+AK+L+ N   + A       P+H A   G  ++  LL++ N + N   + G+ P+H+A   GH+  +  L + +            P+  A K G   + +LLL+ + + N                                K   +PL+V+    +LDIV+L+L            + HS A +                 HIA     + V + LL  G S    E + G   +H+A    H ++V  LL +    ++ NK    P+H++    +  +  + +      D +
Sbjct:  312 AAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAA-----------------------------GKNGLTPLHVAVHHNNLDIVKLLLPR--------GGSPHSPAWNGYT------------PLHIAAKQNQVEVARSLLQYGGS-ANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDAT 697          

HSP 2 Score: 136.346 bits (342), Expect = 4.667e-31
Identity = 98/432 (22.69%), Postives = 187/432 (43.29%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-------KTIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +L +   N + +++  + P+H A    N+ + +LL++    +       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ I + L+ +G+ ++A+       IH A + + ++  + L+  +  ++     +  P+H+A   G   +  +L+ +    N+    G  P+H+AC   H+R++E L K     D+     + P+  A  +G   IV+ LL    + N         ++M     H EV       K K   +        K+ ++PL+ + + GH ++V+L+L+          NN + +         L          HIA   GH+  V  LL+   S   C    G   +H+A  +  V +   LL+R+   +   K    P+H+    +   I+ L L R  S
Sbjct:  191 VLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKA-------KDDQTPLHCAARIGHTNMVKLLLE----------NNANPNLATTAGHTPL----------HIAAREGHVETVLALLEKEASQA-CMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGS 594          

HSP 3 Score: 136.346 bits (342), Expect = 5.729e-31
Identity = 103/471 (21.87%), Postives = 200/471 (42.46%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRI----DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            + V  LL  +  ++    +N   P+H A    NV +V LL+     IE +   ++ P+H A + G++ I ++L+ +G  +     +G SP H+A +  HLD                                 + K L+  G+   +R  N F+   +H AC+ N + + + L+    +++A       P+H+A   G   I   L+Q+    N  N     P+H+A   GH  + ++L + K   ++    +  P+  A ++G  ++V+LLL+NN N N      H  ++        + +    ++        K+  +PL+V+ + G + + EL+L+           + H +A        L          H+A    +L++V+ LL  G S       +G   +HIA   + V++ R LL      +  + +   P+H+     +A ++ L LS+  + +L ++
Sbjct:  219 LNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTP--LHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLE----------RDAHPNAAGKNGLTPL----------HVAVHHNNLDIVKLLLPRGGSP-HSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNK 666          

HSP 4 Score: 119.398 bits (298), Expect = 7.777e-26
Identity = 105/468 (22.44%), Postives = 200/468 (42.74%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD----------------LEARNNFSQYLI-------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            +A  +G+L + L+ L R   +I   N+N    +H A    +V +V  L+  +  +E    +    +H+A   G  ++V+ L++ G +++     G +P ++A +  HL+++KFL+ NG++                 +   N   +LI             H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +   VN     G  P+H+A   G++ ++  L       ++     + P+  A + G   I ++LLD+       I  + K  +  +  + +   +D  ++ + +  EI+       +PL+V+   GH  + +++LD   + N R L                        F   HIAC   H+ V++ LL  G S I+    SG   +H+A    H+ IV+ LL R    ++ N + + P+HM   + +  +    L
Sbjct:   18 RAARSGNLDKALDHL-RNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGA----PIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNG----------------------FTPLHIACKKNHVRVMELLLKTGAS-IDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 457          

HSP 5 Score: 117.472 bits (293), Expect = 2.959e-25
Identity = 105/463 (22.68%), Postives = 208/463 (44.92%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V+ LL ++   +E   +     +H A       +V  L++   ++ A++ +   P+++A +  +L++V+ L+ NG + +V   DG +P  +A + GH +++  LI+ G+  + R       +H A R +    A  L+ N+ N +  +     P+HIA  Y   ++  LL+ +   VN     G  P+H+A   G++ ++  L       ++     + P+  A + G   I ++LLD+       I  + K  +  +  + +   +D  ++ + +  EI+       +PL+V+   GH  + +++LD   + N R L            N     E ++K  + ID   E   T  H+A   GHL +V+ LL  G S     ++  +  +H+A    H ++ ++LL      +   K  Q P+H      + +++ L L  N + +L+
Sbjct:   47 NGLHLASKEGHVKMVVELLHKEII-LETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVR----LPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGA----PIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASP-NVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLA 499          

HSP 6 Score: 107.071 bits (266), Expect = 5.444e-22
Identity = 97/417 (23.26%), Postives = 182/417 (43.65%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD--ARLR-NVRNLTNNCHSSAE-DMIKCQSL-------IDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            + N  DI   N   +  +HLA K G++ +V  L+     L      GN+  H+A   G  ++++ L++ G+++ A++      ++ A + N +E+ KFL+ N  N          P+ +A + G E++ + LI          +YG +       +H+A  N   R    L +   + D  +     P+  A      ++ QLLL+   + N   +     +  L  + +   +   ++ +    +IE       +PL+ + + GH+ I E++LD  A ++   +N  +  H +A+ D + C  L       IDD      T  H+A   GH  V + LLD G +      ++G   +HIAC  +HV ++  LL      D + +    P+H+     +  I+   L R  S ++S+
Sbjct:   32 LRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLI----------NYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVN---FTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKG-AKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSN 434          

HSP 7 Score: 107.071 bits (266), Expect = 5.444e-22
Identity = 63/254 (24.80%), Postives = 116/254 (45.67%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            A   G +  VL LL ++ +    + +  + P+H A     V + ELL+       A     + P+H+A  + NLDIV+LL+  G        +G +P H+A +   +++ + L+  G    A +      +H A +    E+   L+S   N    N     P+H+  + G   +  +LI+    V+A    G  P+H+A   G++++++ L + + D ++       P+  A + G   IV LLL N  + N+
Sbjct:  510 AAREGHVETVLALLEKEASQAC-MTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNE 762          

HSP 8 Score: 106.686 bits (265), Expect = 5.927e-22
Identity = 59/238 (24.79%), Postives = 108/238 (45.38%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            + V  LL  +  +     +N   P+H A+ ++N+ IV+LL+       +       P+H+A K   +++ + L+  G   +   + G +P HLA + GH +++  L+S  ++    N      +H   +   + +A  LI +   ++A       P+H+A  YG   +   L+Q   DVNA+   G  P+H A   GH  I+  L K     +  +     P+  A +LG  S+  +L
Sbjct:  549 VRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 786          
BLAST of SMED30018624 vs. UniProt/SwissProt
Match: sp|O70511|ANK3_RAT (Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE=1 SV=3)

HSP 1 Score: 137.117 bits (344), Expect = 3.617e-31
Identity = 95/430 (22.09%), Postives = 192/430 (44.65%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +V  +L  K  N      N + P+H A   + + ++ELL+ +   I+A  +  + PIH+A   G+++IV  L+ +G   +  ++ G +  H+A   G  +++++L+ +G+ +EA+    Q  +H + R  + +I + L+    +  A       P+H++   G ED+ + L+     ++     G  P+H+A   G L +   L +     D+A    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  + ++++ Q GH+D+V L+L +R  NV NL+N    +                    H+A     +NV + L++ G + ++ +   G   +H+ C + ++ IV FLL  +   +   K    P+H      +  I+ + L  N S
Sbjct:  389 KVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPL-HIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL-SRNANV-NLSNKSGLTP------------------LHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 796          

HSP 2 Score: 122.094 bits (305), Expect = 1.292e-26
Identity = 101/444 (22.75%), Postives = 189/444 (42.57%), Query Frame = 2
Query:   98 LLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKC--DIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            LL Q   N D IE   ++ R T       E+ + P+H A    N+ +  LL++     D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+   + + ++       +H A + + +   + L+ +N  ++     Y   +H+A   G   +  +L+ +  + NA+   G  P+H+AC    +R++E L K      +     + PI  A  +G  +IV  L+ +  + N T  +  +   H+        + R+ +  G       K+ ++PL++S + G  DIV+ +L           N   +S    +               H++   GH +V  +LLD G S +      G   +H+A  +  +++   LL ++   D   K    P+H+        +  L L +  S
Sbjct:  220 LLLQNDTNAD-IESKMVVNRAT-------ESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARND--ITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTT-NVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGAS-----PNAATTSGYTPL---------------HLSAREGHEDVAAFLLDHGAS-LSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGAS 631          

HSP 3 Score: 114.005 bits (284), Expect = 4.142e-24
Identity = 99/439 (22.55%), Postives = 194/439 (44.19%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGN--SPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            ++ LL  +   I+    +   P+H    + +  +VE+L+     I ++    + P+H+A +  +L+ VQLL+ +  ++ V+D+  +  +  H+A   GH  + K L+   ++  A+  N F+   +H AC+ NRI + + L+ +  +++A       PIH+A   G  +I S L+      N  N  G   +H+A  +G   ++ +L +     ++    +  P+  + +LG   IVQ LL    + N      +  + HL   +  + +  F +  G    I +     PL+V+ + G L++  L+L               S++ D      L          H+A    +  V   LLD G S       +G   +HIA   + +DI   LL+     + + ++    +H+     +  ++ L LSRN + +LS++
Sbjct:  291 MVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH--NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTP--LHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPL-HLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQ-------------KSASPDAAGKSGLTP-------LHVAAHYDNQKVALLLLDQGASP-HAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNK 703          

HSP 4 Score: 111.694 bits (278), Expect = 2.402e-23
Identity = 104/462 (22.51%), Postives = 201/462 (43.51%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNC--NLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCD--------VNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTI-----------YMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKC------QSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASIL 1381
            +A   G L + L+ + +   ++   N+N    +H A    +V +V  L+  + +++A   +    +H+A   G  ++V++L++NG +++    +G +P ++A +  HL++++FL+ NG+   L   + F+   +  A +    ++   L+ N+    +  P       +HIA         +LL+Q + +        VN     G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD     +                 H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L   +  V ++TN+  ++      C      + L+D +              HIAC    + V++ LL  G S I+    SG   IH+A    HV+IV  L+      +  N R +  +HM   S  A ++
Sbjct:   47 RAARAGHLEKALDYI-KNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAV--ALQQGHDQVVSLLLENDTKGKVRLP------ALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEML-------LDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNV-PVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGAS-IQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVV 490          

HSP 5 Score: 105.145 bits (261), Expect = 2.067e-21
Identity = 63/238 (26.47%), Postives = 111/238 (46.64%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV +LL +K+ + +   ++   P+H A    N  +  LL+       A       P+H+A K   +DI   L+  G D +     G +  HLA + GH+D++  L+S  +++   N      +H A + +R+ +A+ L++   +++A       P+H+ C YG   I + L+Q +  VNA+   G  P+H A   GH  II  L +     +         +  A +LG  S+V  L
Sbjct:  586 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 823          

HSP 6 Score: 100.908 bits (250), Expect = 4.804e-20
Identity = 54/226 (23.89%), Postives = 103/226 (45.58%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            + P+H A     + +  LL+      +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   +DI   L+  G+D  A        +H A +   +++   L+S N N+   N     P+H+A +    ++  +L+ Q   V+A+   G  P+H+ C  G+++I+  L +     ++       P+  A + G   I+ +LL NN + N+
Sbjct:  574 FTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNE 799          

HSP 7 Score: 86.6557 bits (213), Expect = 8.674e-16
Identity = 79/349 (22.64%), Postives = 140/349 (40.11%), Query Frame = 2
Query:  410 ACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEH-------KEVYHLFYSKKTKRIDRFKMHIGHHKEIE-SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSF-DLSDEY 1429
            A   GHL+     I NG D+   N      +H A +   +E+   L+    N++A   K +  +HIA   G  ++  +L+    +VNA++  G  P+++A    HL ++  L         A      P+  A + G   +V LLL+N+      +   H        +   L     T      KM +    E   +PL+++   G++++  L+L+              ++A D      +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR        K    P+HM     + + + L L  N    D++++Y
Sbjct:   48 AARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLN-------------RAAAVDFTARNDITP-------LHVASKRGNANMVKLLLDRG-AKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY 375          
BLAST of SMED30018624 vs. TrEMBL
Match: A0A182GGY8 (ANK_REP_REGION domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG009719 PE=4 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 7.506e-52
Identity = 131/475 (27.58%), Postives = 241/475 (50.74%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLL-----------DNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYK-------SINSYKKSEKLEK 1471
            L +AC NG   EV+ +L ++  NI+ + E  + P+H A  N +  +VE+LI  K +I+A  ++K  P+HLAC+ G+ ++V++LI    ++   +    +P H AC  GH ++++ LI    ++EA N  +   +H +CR    E+ + LI    N+EA N     P+H+AC+ G +++  +LI++  ++ A N Y   P+H+A  NGH +++E L + K + ++    N  P+  AC+ G K +V++L+           DN    +K     H EV  +   +K   ID         ++  +PL+++CQ GH ++VE+++  +  N+  LT                   EK+    H+AC +GH  VV+ L+    ++IE  +      +H+AC   H ++V+ L+      D + ++   P+H+     +  ++ +FL +N +    D+ K       S N + + EK+ K
Sbjct: 1337 LHKACQNGH-AEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILIRGKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKA-NIDALA------EKKFTPLHLACQNGHKEVVEILIGEK-ANIDALT-------------------EKKSTPLHLACQNGHKEVVEILVGEK-ANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANIDALTQKKNTPLHLACQRGHKEVVEIFLRKNANIAALDDEKCTPLNLASQNGHTEVEKIMK 1782          

HSP 2 Score: 196.052 bits (497), Expect = 3.918e-47
Identity = 128/469 (27.29%), Postives = 237/469 (50.53%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLL-----------DNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL--DARLR--NVRNLT-------NNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKS 1435
            L  AC++G + EV+ +L     NI+ + +N   P+HFA  N    +VE+LI  K +IEA N     P+HLAC+ G+ ++V++LI    ++        +P HLAC  GH + ++ LI   ++++A +      +H AC+    E+ + L+    N++A   K   P+H+AC+ G +++  +LI +  +++A     + P+HLAC NGH  ++E L + K + ++ N  N  P+ +AC  G K +V++L+           DN    + +    HKEV  +   +K   I+        +++  +PL+++CQ GH ++VE+++  +A +   N  N T       N      E +I+ ++ I+   +   T  H+AC +GH  VV+ L+  G ++IE         +H AC   H ++V  L+      D + ++   P+H+   + +  ++ + +    + D   E KS
Sbjct: 1205 LHWACISG-VKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKV-NIEAL------NQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILIR-GKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKS 1664          

HSP 3 Score: 195.667 bits (496), Expect = 4.877e-47
Identity = 129/493 (26.17%), Postives = 241/493 (48.88%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNT---NKTIYME--------HKEVYHLFYSKKT-----KRIDRFKMHI----GHHKEIE-----------------SPLYVSCQRGHLDIVELILDARLRNVRNLT-----------NNCHSS-AEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            K   L  AC NG   EV+ +L R+  NIE +N++ + P+H A  N +  +VE+LI    +I+A  + K  P+HLAC  G+ + VQ+LI    ++   D +  +P H AC+ GH ++++ L+   ++++A        +H AC+    E+ + LI    N++A   K   P+H+AC+ G +++  +LI+   ++ A N     P+H AC NGH  ++E L + K + ++ N  N  P+  +C+ G K +V++L+    N    N+  +          HKEV  +   ++       + +   +HI    GH K +E                 +PL+++C+ GH ++VE+++  +  N+  LT            N H+   E ++K ++ ID   E+  T  H+AC +GH  VV+ L+    ++I+         +H+AC   H ++V  L+      + +N+    P+H+     +  ++ + +    + D
Sbjct: 1234 KSTPLHFACQNGRK-EVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILIRGK-ANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEK-ANIDALTEKKSTPLHLACQNGHKEVVEILVGEKANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANID 1723          

HSP 4 Score: 153.295 bits (386), Expect = 8.179e-34
Identity = 104/446 (23.32%), Postives = 209/446 (46.86%), Query Frame = 2
Query:  203 IHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYME-----------HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRL--IHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYK----SINSYKKSEKLEKALRIEK 1489
            +H A  +  +  V+ LI+N  ++ A +      +H++   G+  IV+LL+ NG +++  D +  +P H AC  G  ++++ LI   ++++A        +H AC+  R E+ + LI    N+EA N     P+H+AC+ G +++  +LI++N +++A   +   P+HLAC  GH   ++ L   K + D+ +     P+  AC+ G   +V++L+    N +     +           HKEV  +   +K   ID         ++  +PL+++CQ GH ++VE+++  +           +  A + +    L          H AC +GH  VV+ L+      +  E ++ D    +H++C   H ++V  L+      + +N+    P+H+     +  ++ + +    + +  ++Y      I S+   EK+ + L  EK
Sbjct: 1139 MHIAAGDGIIEEVKRLINNGANVNAPSVAGYTALHISASSGHRPIVELLLDNGANVNAADTNEYTPLHWACISGVKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKA-NIDALT------EKKSTPLHLACQNGHKEVVEILIRGK----------ANIEALNQVNWTPL----------HFACHNGHKEVVEILIR---EKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREK 1554          

HSP 5 Score: 129.798 bits (325), Expect = 1.686e-26
Identity = 108/452 (23.89%), Postives = 204/452 (45.13%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDL--SVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK-------TIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELI------LDA----RLRNVRNLTNNCHSS-AEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            ++ +L  K  +I   N   +  +H+A+ +  +AI + LI    ++  +  +   P++LA   G+ ++V LLI    +   S N +D  S    A ++GH  +   L     +     +    L+H A     IE  K LI+N  N+ AP+      +HI+   G   I  LL+    +VNA ++    P+H ACI+G   ++E L   K + D+       P+ +AC+ G K +V++L+    N           +++     HKEV  +   K+   ID    H        +PL+++C RGH + V+++      +DA    +   +     N H+   E ++K ++ ID   E+  T  H+AC +GH  VV+ L+    ++I+         +H+AC   H ++V  L+      + +N+    P+H    + +  ++ + +    + +
Sbjct: 1018 LVRILLEKGADINIRNTEGWSALHYAVHHKRIAISKFLIDKGINVNYQTREGKTPLNLAAARGHTEVVILLIEKLIEQFPSRNIID--SAFQTAVQYGHSSVQDMLKRKFPEHAFTKDIDGPLMHIAAGDGIIEEVKRLINNGANVNAPSVAGYTALHISASSGHRPIVELLLDNGANVNAADTNEYTPLHWACISGVKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILI-KENANIDALTEH------KYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEK-ANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIE 1459          

HSP 6 Score: 107.457 bits (267), Expect = 1.057e-19
Identity = 98/461 (21.26%), Postives = 194/461 (42.08%), Query Frame = 2
Query:  191 LYRP-----IHFAITNSNVAIVELLISN-KCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLI----------------------------SNNCNLEAPNYKYDRPI-----HIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKS 1435
             +RP     +  AI N +  I   LI++ K   + + D  +  +  A  YG+ ++V++L+  G D+++ + +G S  H A     + I KFLI  G ++  +    +  ++ A      E+   LI                             +    + P + + + I     HIA   G  +    LI    +VNA +  G   +H++  +GH  I+E L     + ++A+     P+ +AC  GVK +V++L+    N +    +   +   L ++ +  R +  ++ I     IE       +PL+++CQ GH ++VE+++     N+  LT + ++                     H+AC+ GH   VQ L+    ++I+  D +    +H AC   H ++V  L+      D + ++   P+H+   + +  ++ + +    + D   E KS
Sbjct:  964 FFRPNGTKMLKIAIENGSDKICAYLINDCKVHPDVKFDGGLAALIWAVIYGHKNLVRILLEKGADINIRNTEGWSALHYAVHHKRIAISKFLIDKGINVNYQTREGKTPLNLAAARGHTEVVILLIEKLIEQFPSRNIIDSAFQTAVQYGHSSVQDMLKRKFPEHAFTKDIDGPLMHIAAGDGIIEEVKRLINNGANVNAPSVAGYTALHISASSGHRPIVELLLDNGANVNAADTNEYTPLHWACISGVKEVVEILIGGKANIDA---LAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKEN-ANIDALTEHKYTP-------------------LHLACARGHKEAVQILIGEK-ANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKS 1400          
BLAST of SMED30018624 vs. TrEMBL
Match: A0A355KTI7 (ANK_REP_REGION domain-containing protein OS=Candidatus Dependentiae bacterium OX=2030827 GN=DDZ86_04425 PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 4.719e-51
Identity = 126/435 (28.97%), Postives = 228/435 (52.41%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFY--SKKTKRIDRFKMHIGHHKEI-----ESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL 1417
            +++ LL  K  N+   N+N   P+H A    N  IV L+++   D+ + ++    P+H+AC    LDI++LL+  G +++  + +G +P H+AC  G+L+I+K L++ G+ +   +   Q  ++ AC  + + I K LI+ N N+   N   + P+HIAC +G++D+  LL++++  VN  N+ G  P++ AC NG+L II+ L     D +SA+     P+  AC LG   IV+LL++ + N N T   +  E   L++  SK    I +  ++ G    I     ++PLY +    + +I++L+L+ +  NV                    I D+      + ACS G+L +V+ LL+ G ++++  +  G+  ++ AC   +++IV+ LL++    +I NK    P+H+  S  Y  I+ L L    +  L
Sbjct:  142 DIVQLLLEKGANVNSANKNGKTPLHGACFFDNFDIVNLVLNEGADVNSADEDGNTPLHIACFNNKLDIIKLLLEKGANVNSANKNGETPLHIACSHGYLEIVKLLLNKGAKVNIADENGQTPLYWACSNDYVNIVKLLINENANVNITNKNGETPLHIACSHGYKDVVQLLLEKSAKVNIANNNGQTPLYWACSNGYLDIIKLLLNEGADVNSADKDGNTPLHKACHLGYLEIVKLLINEDANVNIT---DKDEWTPLYWACSKGYLEITKLLLNKGAKVNIANGNGKTPLYFAFSNNYKEIIKLLLE-KGANVN-------------------ITDKDGVTLLYWACSEGYLEIVKLLLEKG-ANVDIANEYGNTSLYPACSKGYLEIVKLLLEKGANVNITNKNGNAPLHIACSKGYLEIVKLLLENGTNPTL 552          

HSP 2 Score: 192.2 bits (487), Expect = 4.514e-47
Identity = 116/420 (27.62%), Postives = 215/420 (51.19%), Query Frame = 2
Query:  185 ENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEI-------ESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            EN   P+H A +  +   V  L+ N   + + N+    P+H+AC    LDI QLL+  G  ++  + +G +P +LAC  G+ +IIK L+  G+++ + NN  +  +H AC  +  +I + L+    N+ + N     P+H AC +   DI +L++ +  DVN+ +  GN P+H+AC N  L II+ L +   + +SAN     P+  AC  G   IV+LLL+     N  I  E+ +   L+++     ++  K+ I  +  +       E+PL+++C  G+ D+V+L+L+   +   N+ NN   +                    + ACS+G+L++++ LL+ G +D+   D  G+  +H AC   +++IV+ L++ +   +I +K    P++   S  Y  I  L L++    ++++
Sbjct:   27 ENENSPLHGACSFGDFNTVNDLLENGAKVNSANNNGETPLHIACSNNKLDIAQLLLEKGAKVNSANNNGETPLYLACSHGYKEIIKLLLEKGANVNSANNNGETPLHGACFHSYSDIVQLLLEKGANVNSANKNGKTPLHGACFFDNFDIVNLVLNEGADVNSADEDGNTPLHIACFNNKLDIIKLLLEKGANVNSANKNGETPLHIACSHGYLEIVKLLLNKGAKVN--IADENGQT-PLYWACSNDYVNIVKLLINENANVNITNKNGETPLHIACSHGYKDVVQLLLEKSAK--VNIANNNGQTP------------------LYWACSNGYLDIIKLLLNEG-ADVNSADKDGNTPLHKACHLGYLEIVKLLINEDANVNITDKDEWTPLYWACSKGYLEITKLLLNKGAKVNIAN 422          

HSP 3 Score: 150.599 bits (379), Expect = 1.812e-33
Identity = 95/320 (29.69%), Postives = 168/320 (52.50%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILD 1036
            E  L  AC +G L E++ LL  K   +   +EN   P+++A +N  V IV+LLI+   ++   N     P+H+AC +G  D+VQLL+     +++ + +G +P + AC  G+LDIIK L++ G+D+ + +      +H AC    +EI K LI+ + N+   +     P++ AC  G+ +I  LL+ +   VN  N  G  P++ A  N +  II+ L +   + +  +   +  + +AC  G   IV+LLL+   N +      +  +Y    SK    I +  +  G +     K   +PL+++C +G+L+IV+L+L+
Sbjct:  228 ETPLHIACSHGYL-EIVKLLLNKGAKVNIADENGQTPLYWACSNDYVNIVKLLINENANVNITNKNGETPLHIACSHGYKDVVQLLLEKSAKVNIANNNGQTPLYWACSNGYLDIIKLLLNEGADVNSADKDGNTPLHKACHLGYLEIVKLLINEDANVNITDKDEWTPLYWACSKGYLEITKLLLNKGAKVNIANGNGKTPLYFAFSNNYKEIIKLLLEKGANVNITDKDGVTLLYWACSEGYLEIVKLLLEKGANVDIANEYGNTSLYPAC-SKGYLEIVKLLLEKGANVNITNKNGNAPLHIACSKGYLEIVKLLLE 545          

HSP 4 Score: 124.405 bits (311), Expect = 2.929e-25
Identity = 77/257 (29.96%), Postives = 136/257 (52.92%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN 862
            E  L  AC +G   +V+ LL  K+  +   N N   P+++A +N  + I++LL++   D+ + +     P+H AC  G L+IV+LLI+   ++++ D D  +P + AC  G+L+I K L++ G+ +   N   +  ++ A   N  EI K L+    N+   +      ++ AC  G+ +I  LL+++  +V+  N YGN  ++ AC  G+L I++ L +   + +  N     P+  AC  G   IV+LLL+N  N
Sbjct:  294 ETPLHIACSHG-YKDVVQLLLEKSAKVNIANNNGQTPLYWACSNGYLDIIKLLLNEGADVNSADKDGNTPLHKACHLGYLEIVKLLINEDANVNITDKDEWTPLYWACSKGYLEITKLLLNKGAKVNIANGNGKTPLYFAFSNNYKEIIKLLLEKGANVNITDKDGVTLLYWACSEGYLEIVKLLLEKGANVDIANEYGNTSLYPACSKGYLEIVKLLLEKGANVNITNKNGNAPLHIACSKGYLEIVKLLLENGTN 549          

HSP 5 Score: 97.8265 bits (242), Expect = 5.801e-17
Identity = 56/219 (25.57%), Postives = 113/219 (51.60%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK 757
            L +AC  G L E++ LL  +  N+   +++ + P+++A +   + I +LL++    +   N     P++ A      +I++LL+  G ++++ D DG +  + AC  G+L+I+K L+  G++++  N +    ++ AC    +EI K L+    N+   N   + P+HIAC  G+ +I  LL++   +   + + G  P++L  I     ++  L   K
Sbjct:  363 LHKACHLGYL-EIVKLLINEDANVNITDKDEWTPLYWACSKGYLEITKLLLNKGAKVNIANGNGKTPLYFAFSNNYKEIIKLLLEKGANVNITDKDGVTLLYWACSEGYLEIVKLLLEKGANVDIANEYGNTSLYPACSKGYLEIVKLLLEKGANVNITNKNGNAPLHIACSKGYLEIVKLLLENGTNPTLKPNNGKSPLNLLNIKQLENLLTSLIPTK 580          
BLAST of SMED30018624 vs. TrEMBL
Match: Q16FI9 (AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014742 PE=4 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 1.942e-48
Identity = 117/469 (24.95%), Postives = 237/469 (50.53%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE------------------SPLYVSCQRGHLDIVELILDARLRNVRNLTN-----------NCH-SSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            A  NG L EV+ LL     N++      + P+H A  N ++ +V+LLI N+ +++ + ++   P+H A + G+L++V+ LI N  ++     +G +P H+A + GHL+++K LI N ++++ + N     +H A +   +E+ KFLI N  N++    +   P+H+A E G  ++  LLI+   +V+ + + G  P+H+A  NGHL +++ L   + + D+  Y    P+  A + G   +V+LL+DN  N + T   ++K +  L ++ +   ++  K+ I +   +                   +PL+ + Q GHL++V+L+++ R  NV    N           N H    + +I+ ++ +D  +    T  ++A  +GHL VV+ L++   ++++     G   +++A    H+++V+ L+D     D        P+H+   + +  ++ L +    + D +
Sbjct:   92 AAENGHL-EVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTT---QNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENR-ANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLIN-NRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTT 554          

HSP 2 Score: 194.512 bits (493), Expect = 1.080e-47
Identity = 112/458 (24.45%), Postives = 227/458 (49.56%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLF-----------KVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            A  NG L EV+  L     N++   +  + P+H A  N ++ +V+LLI N+ +++ + ++   P+H A + G+L++V+ LI N  ++     +G +P HLA E GHL+++K LI N ++++ + N     +H A +   +E+ KFLI N  N++   Y+   P+H+A + G  ++  LLI    +V+   + G  P+H A  NGHL +++ L            + + + D+     + P+ +A + G   +V+LL++N  N   T   +++    L ++ +   ++  K+ I +   +++       PLYV+   GHL++V+L+++ R                        +D  +    T  ++A  +GHL VV+ L+D   ++++     G   +H+A    H+++V+ L+D     D    +   P+++   + +  ++ L +    + D +D
Sbjct:  158 ASQNGHL-EVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTT---QNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRAN----------------------VDTTQNEGWTPLYVASKNGHLEVVKLLID-NKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTD 588          

HSP 3 Score: 193.741 bits (491), Expect = 1.804e-47
Identity = 106/411 (25.79%), Postives = 198/411 (48.18%), Query Frame = 2
Query:  167 NIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            NI+  N     P+H A  N ++ +V+LLI N  +++ E D+   P+HLA + G L++V+LLI NG ++     +G +P HLA E GHL+++K LI N ++++ + N     +H A +   +E+ K LI N  N++    +   P+H A + G  ++   LI    +V+     G  P+H+A  NGHL +++ L + + + D+       P+ +A + G   +V+ L+DN  N + T                               E  +PL+++ + GHL++V+L+++ R                        +D +K    T  H+A  +GHL VV++L+D   ++++     G   +H+A    H+++V+ L+D     D    +   P+H    + +  ++ L +
Sbjct:   11 NIDTANNGGRTPLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTT-----------------------------QDEGWTPLHLAAENGHLEVVKLLIENRAN----------------------VDTKKNGGWTPLHVASQNGHLEVVKFLID-NRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLI 369          

HSP 4 Score: 187.193 bits (474), Expect = 2.654e-45
Identity = 115/471 (24.42%), Postives = 232/471 (49.26%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----------------------ARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            L  A  NG L +V+ LL     N++   +  + P+H A  N  + +V+LLI N  +++   D+   P+HLA + G+L++V+LLI N  ++      G +P H+A + GHL+++K LI N ++++ + N     +H A +   +E+ KFLI N  N++    +   P+H+A + G  ++  LLI+   +V+ + + G  P+H A  NGHL +++ L   + + D+       P+  A + G   +V+LL++N  N +     ++     L  + +   ++  K  I +   ++       +PL+V+ Q GHL++V+L++D                        L  V+ L +N  +  + +I+ ++ +D  + +  T  H A  +GHL VV+ L++   +++      G   +H A    H+++V+ L++     D        P+++   + +  ++ L ++   + D +
Sbjct:   23 LHVASQNGHL-KVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVD---TKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIE-NRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTT 488          

HSP 5 Score: 186.037 bits (471), Expect = 6.180e-45
Identity = 119/472 (25.21%), Postives = 231/472 (48.94%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDL-----------SVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNC----HSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            L  A  NG L EV+  L     N++      + P+H A  N ++ +V+LLI NK +++   ++ + P+H A + G+L++V+LLI N  ++                 G +P H A + GHL+++K LI N +++    N     +H A R   +E+ K LI N  N++    +   P+++A   G  ++  LLI    +V+   + G  P+++A  NGHL +++ L   K + D+       P+  A + G   +V+LL+DN  N + T   ++K +  L+ + K   ++  K+ I +   ++       +PL+V+ Q GHL++V+L+++ R  NV    N      H ++++        +I  ++ +D  +    T  H+A  +GHL VV+ L++   ++++     G   +H A    H+++V+ L+D     D        P+H+   + +  ++ L +    + D +
Sbjct:  287 LHVASQNGHL-EVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTT---KNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENR-ANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIE-NRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTT 752          

HSP 6 Score: 185.267 bits (469), Expect = 1.199e-44
Identity = 105/437 (24.03%), Postives = 219/437 (50.11%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            V+ LL     N++        P+HFA  N ++ +V+LLI N+ ++    ++   P+H A + G+L++V+LLI N  ++     +G +P ++A   GHL+++K LI+N ++++   N     ++ A +   +E+ K LI N  N++    +   P+H+A + G  ++  LLI    +V+   + G  P+++A  NGHL +++ L   K + D+ +     P+  A + G   +V+LL++N  N + T   ++K +  L ++ +   ++  K+ I +   ++       +PL+V+ Q GHL++V+L+++ R                        +D  + +  T  H A  +GHL VV+ L+D   ++++     G   +H+A    H+++V+ L+D     D    +   P+++   + +  ++ L +    + D +
Sbjct:  375 VVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTT---QNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRAN----------------------VDTTQNKGITPLHFASQNGHLEVVKLLID-NRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTT 785          

HSP 7 Score: 166.777 bits (421), Expect = 1.274e-38
Identity = 90/318 (28.30%), Postives = 175/318 (55.03%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDAR 1042
            A +NG L EV+ LL     N++      + P++ A  N ++ +V+LLI NK +++   ++   P+H+A + G+L++V+LLI N  ++      G +P ++A + GHL+++K LI N ++++  +N     +H A +   +E+ K LI N  N++    K   P+H A + G  ++  LLI    +V+   + G  P+H+A  NGHL +++ L + + + D+     + P+ +A + G   +V+LL+DN  N + T   +++    L  + +   ++  K+ I +   ++       +PLYV+   GHL++V+L++D R
Sbjct:  466 ASINGHL-EVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTT---QNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNR 779          

HSP 8 Score: 143.665 bits (361), Expect = 2.599e-31
Identity = 78/258 (30.23%), Postives = 138/258 (53.49%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT 874
            L  A  NG L EV+ LL     N++        P++ A  N ++ +V+LLI NK +++  +++   P+H+A + G+L++V+LLI N  ++      G +P H A + GHL+++K LI N ++++   N     +H A +   +E+ K LI N  N++    K   P+H A + G  ++  LLI    +V+   + G  P+H+A  NGHL +++ L   + + D+     + P+  A   G   +V+LL+DN  N + T
Sbjct:  529 LHVASQNGHL-EVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTT 785          

HSP 9 Score: 112.079 bits (279), Expect = 2.152e-21
Identity = 59/196 (30.10%), Postives = 105/196 (53.57%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYG 688
            L  A  NG L EV+ LL     N++        P+HFA  N ++ +V+LLI N+ +++   ++   P+H+A + G+L++V+LLI N  ++      G +P H A + GHL+++K LI N ++++   N     +H A +   +E+ K LI N  N++    K   P+++A   G  ++  LLI    +V+   + G
Sbjct:  595 LHVASQNGHL-EVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTTQNEG 789          

HSP 10 Score: 110.538 bits (275), Expect = 7.272e-21
Identity = 69/300 (23.00%), Postives = 130/300 (43.33%), Query Frame = 2
Query:  533 IAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            + KFLI +N N++  N     P+H+A + G   +  LLI    +V+ E   G  P+HLA  NG+L +++ L     + D+       P+  A + G   +V+LL+DN  N +                  TK+   +           +PL+V+ Q GHL++V+L+++ R                        +D +K    T  H A  +GHL VV++L+D   ++++     G   +H+A    H+++V+ L++     D        P+H    + +  ++   +    + D + +
Sbjct:    1 MVKFLIDHNANIDTANNGGRTPLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVD------------------TKKNGGW-----------TPLHVASQNGHLEVVKLLIENRAN----------------------VDTKKNEGWTPLHFASQNGHLEVVKFLID-NRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQD 248          
BLAST of SMED30018624 vs. TrEMBL
Match: W4YMA4 (ANK_REP_REGION domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 1.975e-48
Identity = 113/452 (25.00%), Postives = 215/452 (47.57%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVR---NLTNNCHSSA--------EDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A L G L +V+  L  +   +E  N N   P+HFA    ++ +V+ L+S    +E  +   ++P+  A   G+LD+V+ L+S G  +   + +G++P H+A + GHLD++++L+S G+ +E  NN     +H A +   ++I ++L+S    +E  N K   P+H A   G  D+   L+ Q   V   N+ G+ P+H A + GHL ++++L       +  N     P+ +A + G   +VQ L+       +  +++  + Y +    + +R D   +          PL  +   GHLD+VE ++    +  R   N T   H ++        + ++   + ++       T  H A   GHL+VVQYL+  G + +E  D +G   +H A    H+D+V++L+ +    +  N +   P+H      +  ++   +S+    +  D+
Sbjct:  944 ASLGGHL-DVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASQGGHLDVVQYLVSQGAQEGRGGHLDVVQ-YLVNQGAEVERGDNNGL---------KPLQDASHEGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQG-AQVERGDKNGSTPLHDASHEGHLDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQVERGDK 1383          

HSP 2 Score: 194.512 bits (493), Expect = 1.005e-46
Identity = 114/462 (24.68%), Postives = 214/462 (46.32%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLL-----------DNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            A L G L +V+  L  +   +E  N N   P+HFA    ++ +V+ L+S    +E  +   ++P+  A   G+LD+V+ L+S G  +   + +G++P H+A + GHLD++++L+S G+ +E  NN     +H A +   ++I ++L+S    +E  N K   P+H A   G  D+   L+ Q   V   N+ G+ P+H A + GHL ++++L       +  N     P  +A + G   +VQ L+           D + +++      H +V     S+  + ++R         +  +PL+ + + GHLDIV+ ++ +R   V    N                   K     H A   GH+++VQYL+  G + +E  + +G   +H A L  H+D+V++L+ +    +  N     P H      +  ++   +S+       +   S +S+  S+ 
Sbjct:  134 ASLGGHL-DVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQ-VERGN------NDGSTPLHFASKGGHLDIVQYLV-SRGAQVERGNN-------------------KGSTPLHDASHKGHIDLVQYLVSQG-AQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQVGRGNNDGSTSSHFASQG 566          

HSP 3 Score: 188.734 bits (478), Expect = 6.181e-45
Identity = 112/473 (23.68%), Postives = 208/473 (43.97%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELIL--DARLRNVRN-----LTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIG-----------------WSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSI 1438
            I+++  L  +   +E  N N   P+HFA    ++ +V+ L+S    +E  N+    P H A + G+LD+VQ L+S G      + DG++ SH A + GHLD++++L+S G+ +E  NN     +H A +   ++I ++L+S    +E  N K   P+H A   G  D+   L+ Q   V   N+ G+ P+H A + GHL ++++L       +  N     P  +A + G   +VQ L+       +     +       ++ +   +D  +  +    ++E       +PL+ +   GHLD+V+ ++   A++    N     L +       D+I+       Q    +       H A   GHL+VVQYL+  G                  + +E  + +G   +H A L  H+D+V++L+ +    +  +    KP+       +  ++   +S+       D   S 
Sbjct:  305 IDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQVGRG---NNDGSTSSHFASQGGHLDVVQYLVSQGAQVEKGDNDGSTPLHFASLGGHLDVVQYLVSQGAQVEKGDNNGWTPLLSASQGGHLDVIQYLVSQGAQVEKGNNDGSTPLHFASQGGHLDVVQYLVSQGAQVERGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGDNNGST 774          

HSP 4 Score: 187.963 bits (476), Expect = 1.264e-44
Identity = 106/439 (24.15%), Postives = 207/439 (47.15%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLL---------DNNCNTNKTIYME--HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            G  + V+  L  +   +E  N N   P+HFA    ++ +V+ L+S    +E  +   ++P+  A   G+LD+V+ L+S G  +   + +G++P H+A + GHLD++++L+S G+ +E  NN     +H A +   +++ ++L+S    +   N     P+H A   G  D+   L+ Q   V   N+ G+ P+H A + GHL ++++L       +  +   ++P++ A   G   +V+ L+         +NN +T   +  +  H +V     S+  + ++R         +  +PL+ + Q GHLDIV+ ++ +R   V    N                   K     H A   GH+++VQYL+  G + +E  + +G   +H A L  H+D+V++L+ +    +  N     P+H      +  ++   +S+  
Sbjct:  782 GGQLNVVKYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQ-VERGN------NDGSTPLHFASQGGHLDIVQYLV-SRGAQVERGNN-------------------KGSTPLHDASHKGHIDLVQYLVSQG-AQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASQGGHLDVVQYLVSQGA 1192          

HSP 5 Score: 184.882 bits (468), Expect = 1.242e-43
Identity = 111/455 (24.40%), Postives = 209/455 (45.93%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDA-----RLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERF--------CTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A L G L +V+  L  +   +E  ++N  +P+  A    ++ +VE L+S    +E  N+    P+H+A + G+LD+V+ L+  G  ++  + +G +P H A   GHLD++++L+S G+ +E  NN     +H A     +++ ++L+S    +E  +    +P+  A   G  D+   L+ Q   V   N+ G+ P+H+A   GHL ++++L       +  N     P+ +A K G   IVQ L+       +     +K    L  +     ID  +  +    ++E       +PL+ +   GHLD+V+ ++       R  N  +  ++  S    +   Q L+    +           +H A   GHL+VVQYL+  G + +E  +  G   +H A    H+DIV++L+ R    +  N +   P+H      +  ++   +S+    +
Sbjct:   35 ASLGGHL-DVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERG---NNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQGGHLDVVQYLVSQG-AQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVE 484          

HSP 6 Score: 182.956 bits (463), Expect = 4.278e-43
Identity = 105/453 (23.18%), Postives = 211/453 (46.58%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-----NTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-------NVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            ++V+  L  +   +E  N N   P+HFA    ++ +V+ L+S   ++E  ++  ++P+  A   G+LD+V+ L+S G  ++  D +G++P  +A + G L+++K+L+S G+ +E  NN     +H A     +++ ++L+S    +E  +    +P+  A   G  D+   L+ Q   V   N+ G+ P+H+A   GHL ++++L       +  N     P+ +A + G   +V+ L+         N N T          L ++     +D  +  +    ++E       +PL+ +   GHLD+V+ ++    +        ++ L +  H    D+++       + ER         H+A   GHL+VVQYL+  G + +E  +  G   +H A    H+DIV++L+ R    +  N +   P+H      +  ++   +S+    +
Sbjct:  686 LDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGDNNGSTPLIMASQGGQLNVVKYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDVVEYLVSKGAQVTRGNNNGTTP--------LHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQG-AQVERGNNDGSTPLHFASQGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVE 1129          

HSP 7 Score: 179.874 bits (455), Expect = 3.864e-42
Identity = 116/527 (22.01%), Postives = 221/527 (41.94%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWN-------------------MRPIEYACKLGVKSIVQLLLDNNC-----NTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIG--------------------------------WSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            A L G L +V+  L  +   +E  ++N  +P+  A    ++ +VE L+S    +E  N+    P+H+A + G+LD+VQ L+S G  +   + DG++P H A + GHLDI+++L+S G+ +E  NN     +H+A     I++ ++L+S    +E  N     P+H A   G  D+   L+ Q   V   N+ G+ P+H A   GHL ++++L           + +                   ++P++ A   G   +V+ L+         N N T          L ++     +D  +  +    ++E       +PL+ +   GHLD+V+ ++       R  +N +   H ++ +        ++   + ++    +  T  H A   GH +VVQYL+  G                                 + +E  +  G   +H+A    H+D+V++L+ +    +  N     P+H      +  I+   +SR    +  +   S + +  S K
Sbjct:  977 ASLGGHL-DVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASQGGHLDVVQYLVSQGAQEGRGGHLDVVQYLVNQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGNNNGTTP--------LHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGSTPLHDASHEGHLDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNDGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTSLHDASHK 1494          

HSP 8 Score: 172.555 bits (436), Expect = 7.489e-40
Identity = 107/467 (22.91%), Postives = 204/467 (43.68%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNC-----HSSAEDMIKCQSLIDD--EKERF------CTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            G  + V+  L  +   +   N N  +P+HFA    ++ +V+ L+S    +E  +   ++P+  A   G+LD+V+ L+S G  +   + +G++P H+A + GHLD++++L+  G+ +   NN     +H A     +++ ++L+S    +E  N     P+H A   G  D+   L+ Q   V   +  G +P+  A   GHL ++E+L       +  N     P+  A + G   +VQ L+       +     +     L ++ K   +D  +  +    ++E       +PL+ +  +GH+D+V+ ++    +  R   N        S    +   Q L+    + ER        +H A   GHL+VVQYL+  G  +    +  G    H A    H+D+V++L+ +    +  N     P+H      +  I+   +SR    +  +   S   +  S K
Sbjct:    5 GGQLNVVKYLVSQGAQVVRGNNNGSKPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERG---NNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNN-DGSTSSHFASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHK 467          

HSP 9 Score: 170.244 bits (430), Expect = 4.194e-39
Identity = 103/438 (23.52%), Postives = 192/438 (43.84%), Query Frame = 2
Query:   98 LLSQACLNG--DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            L+SQ    G    ++V+  L  +   +E  + N  +P+  A    ++ +VE L+S    +   N+    P+H A   G+LD+VQ L+S G  +   + +G++P H A   GHLD++++L+S G+ +E  +      +H+A     +++ ++L+S    +E  N K   P+H A   G  D+   L+ Q   V   +  G +P+  A   GHL ++E+L       +  N     P+  A + G   +VQ L+                        +  +++R         +  +PL+ + + GHLDIV+ ++ +R   V    N                   K     H A   GH+++VQYL+  G + +E  + +G   +H A L  H+D+V++L+ +    +  N     P H      +  ++   +S+  
Sbjct: 1187 LVSQGAQEGRGGHLDVVQYLVNQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGSTPLHDASHEGHLDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNDGSTPLHVASQGGHLDVVQYLV-----------------------SQGAQVERGN------NDGSTPLHFASKGGHLDIVQYLV-SRGAQVERGNN-------------------KGSTSLHDASHKGHIDLVQYLVSQG-AQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGA 1574          

HSP 10 Score: 169.859 bits (429), Expect = 5.118e-39
Identity = 109/474 (23.00%), Postives = 201/474 (42.41%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGC-------------------DLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK-----------TIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            I+++  L  +   +E  N N   P+HFA    ++ +V+ L+S    +E  N+    P+H A + G+LD+VQ L+S G                    ++   D +G  P   A   GHLD++++L+S G+ +   NN     +H A     +++ ++L+S    +E  N     P+H A   G  D+   L+ Q   V   +  G+ P+H A   GHL ++++L       +  N     P+ +A   G   +VQ L+       +             +  H +V     S+  + ++R         +  +PL+V+ Q GHLD+V+ ++    +  R   NN  S+                    H A   GHL++VQYL+  G + +E  +  G   +H A    H+D+V++L+ +    +  N     P+H      +  ++   +S+    +  +   S  S+  S+ 
Sbjct: 1115 IDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASQGGHLDVVQYLVSQGAQEGRGGHLDVVQYLVNQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGSTPLHDASHEGHLDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQ-VERGN------NDGSTPLHVASQGGHLDVVQYLVSQGAQVERG--NNDGST------------------PLHFASKGGHLDIVQYLVSRG-AQVERGNNKGSTSLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQG 1560          

HSP 11 Score: 169.859 bits (429), Expect = 5.677e-39
Identity = 113/507 (22.29%), Postives = 218/507 (43.00%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIAC------------------EYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLL---------DNNCNTN-------------KTIYMEHKEVYH--------LFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            A L G L +V+  L  +   +E  N +   P HFA    ++ +V+ L+S    +   N+      H A + G+LD+VQ L+S G  +   D DG++P H A   GHLD++++L+S G+ +E  +N     + +A +   +++ ++L+S    +E  N     P+H A                   E G  D+   L+ Q   V   N+ G+ P+H A + GHL ++++L     + +  +   ++P++ A   G   +V+ L+         DNN +T              K +  +  +V          L ++     +D  +  +    ++E        PL  +   GHLD+VE ++       R   N +   H +++         ++   + ++       T  H A   GHL+VV+YL+  G + +   + +G   +H A L  H+D+V++L+ +    +  N     P+H      +  ++   +S+    +  D+
Sbjct:  497 ASLGGHL-DVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQVGRGNNDGSTSSHFASQGGHLDVVQYLVSQGAQVEKGDNDGSTPLHFASLGGHLDVVQYLVSQGAQVEKGDNNGWTPLLSASQGGHLDVIQYLVSQGAQVEKGNNDGSTPLHFASQGGHLDVVQYLVSQGAQVERGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGDNNGSTPLIMASQGGQLNVVKYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASQGGHLDVVEYLVSKG-AQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDK 1001          

HSP 12 Score: 162.155 bits (409), Expect = 1.465e-36
Identity = 106/450 (23.56%), Postives = 191/450 (42.44%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFA------------------ITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL-EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            G  ++V+  L  +   +E+ N +   P+HFA                  +   ++ +V+ L+S    +E  N+    P+H A   G+LD+VQ L+S G ++   D +G  P   A   GHLD++++L+S G+ +    NN S  LI  A +  ++ + K+L+S    +E  N     P+H A   G  D+   L+ Q   V   +  G +P+  A   GHL ++E+L       +  N     P+  A + G   +VQ L+                        +  +++R         +  +PL+ + Q GHLD+VE ++    +  R   N                         H A   GHL+VVQYL+  G + +E  + +G   +H A L  H+D+V++L+ +    +  +K   KP+       +  ++   +S+    +
Sbjct:  632 GGHLDVIQYLVSQGAQVEKGNNDGSTPLHFASQGGHLDVVQYLVSQGAQVERGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAEVERGDNNGLKPLQDASHEGHLDVVEYLVSKGAQVTRGDNNGSTPLI-MASQGGQLNVVKYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVQYLV-----------------------SQGAQVERGN------NDGSTPLHFASQGGHLDVVEYLVSKGAQVTRGNNNGT--------------------TPLHFASLGGHLDVVQYLVSQG-AQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVE 1030          

HSP 13 Score: 147.517 bits (371), Expect = 5.417e-32
Identity = 68/245 (27.76%), Postives = 124/245 (50.61%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNT 865
            ++V+  L  +   +E  N     P+HFA    +  +V+ L+S    +E  +   ++P+  A   G+LD+V+ L+S G  +   + DG++P H+A + GHLD++++L+S G+ +E  NN     +H A +   ++I ++L+S    +E  N K    +H A   G  D+   L+ Q   V   N+ G+ P+H A + GHL ++++L       +  N     P  +A + G   +VQ L+     T
Sbjct: 1332 LDVVQYLVSQGAQVERGNNKGTTPLHFASHEGHFDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNDGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTSLHDASHKGHIDLVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQT 1576          

HSP 14 Score: 144.436 bits (363), Expect = 3.927e-31
Identity = 90/409 (22.00%), Postives = 183/409 (44.74%), Query Frame = 2
Query:  308 LACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK-----------TIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAE----DMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            +A + G L++V+ L+S G  +   + +G+ P H A   GHLD++++L+S G+ +E  +      + +A     +++ ++L+S    +E  N     P+H+A + G  D+   L+ +   V   N+ G  P+H A + GHL ++++L       +  N     P+ +A   G   +VQ L+       +             +  H +V     S+  + ++R            +PL+V+ Q GHLD+V+ ++       R   + +   H +++    D+++       Q    + K     H A   GH+++VQYL+  G + +E  + +G   +H A L  H+D+V++L+ +    +  N     P H      +  ++   +S+       +   S +S+  S+ 
Sbjct:    1 MASQGGQLNVVKYLVSQGAQVVRGNNNGSKPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQVERGNNNGSTPLHVASQGGHLDVVEYLVCKGAQVTRGNNNGTTPLHFASLGGHLDVVQYLVSQGAQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGDKNGLKPLQDASHEGHLDVVEYLVSQGAQ-VERGN------NNGSTPLHVASQGGHLDVVQYLVSQGAQVERGNNDGSTPLHFASKGGHLDIVQYLVSRGAQVERGNNKGSTPLHDASHKGHIDLVQYLVSQG-AQVERGNNNGSTPLHFASLGGHLDVVQYLVSQGAQVERGNNDGSTPSHFASQGGHLDVVQYLVSQGAQEGRGNNDGSTSSHFASQG 401          
BLAST of SMED30018624 vs. TrEMBL
Match: A0A1X7T5X6 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 2.254e-47
Identity = 137/465 (29.46%), Postives = 232/465 (49.89%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISN-KCDIEAENDQKMRPIHLACKYGNLDIVQ-LLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNNFSQYLIHNACRFNRIEIAKFL-ISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQ-NCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK-CDWDSANYWNMRPIEYACKLGVKSIVQLLLDNN-CNTNKTIYMEHKEVYHLFYSKKTKRIDRF----------KMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNV--------RNLTNNCHSSAEDMIK-------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL-LDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLS 1396
            L +AC +G++  V +L+  K  N+     N Y P+H+A    +  IV++L ++ +C+IE E     RP+H AC+ GN+DIV+ L+I   CD++    +G +P H ACE GH +I+K L ++   +LEA  NF+   +H AC    ++I   L I  +C++ A       P+H ACE G  +I  +L     C++ AE+++ NRP+H AC +G++ I+ HL   K CD ++  +    P+ YAC+ G   IV++L +++ CN         + ++    S     +             K  IG+     +PL+ +C++GH + V+++ +    N+        R L   C S   D+++       C             H AC  GH  +V+ L +    +IE ED S DR +H AC   +VDIVR L +D++C  +   +    P+H      +  I+ +  +
Sbjct:   82 LHKACESGNVDIVCHLVIDKHCNVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEVEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGNFNDRPLHKACESGNVDIVHHLVIDKHCDVNAKGKDGYTPLHYACEKGHFEIVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVINKHCDVNAKDRIGY-----TPLHYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEAEDYSKDRPLHNACESGNVDIVRHLVIDKHCDVNAKGRIGYTPLHYACEKGHFEIVKILTN 541          

HSP 2 Score: 192.2 bits (487), Expect = 4.351e-46
Identity = 138/452 (30.53%), Postives = 241/452 (53.32%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISN-KCDIEAENDQKMRPIHLACKYGNLDIVQ-LLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNNFSQYLIHNACRFNRIEIAKFL-ISNNCNLEAPNYKYDRPIHIACEYG-FEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK-CDWDSANYWNMRPIEYACKLGVKSIVQLLLDN-NCNTNKTIYMEHKEVYHLFYSKKTKRIDRFK-MHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNN--CHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDR-NCCYDIMNKRYQKPIHMI--PSSSYASILPLFLS 1396
            L +AC +G++  V +L+  K  ++   +   Y P+H+A    +   V++L ++ +C+IEA+++   RP+H AC+ GN+DIV+ L+I   CD++    +G +P H ACE GH +I+K L ++   +LEA  +F+   +H AC    ++I + L I  +C++ A +     P+H ACE G FE +  L     C++ AE+++ NRP+H AC +G++ I+ HL   K CD ++       P+ YAC+ G   IV+ L ++  CNT      + +    L  + K++ +D  + + I  H+++        +PL+ +C++GH +IV++           LTN+  C++ AED+   +SL          H AC  G L++V+YL+     D+  +  +G   +H AC   H +IV+ L +   C  +  N   + P+H+     SSY S    F S
Sbjct:  558 LHKACESGNVDIVRHLVINKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAKDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKFLTNHPQCNTKAEDVCDDRP---LHKACKSRNVDIVRYLVIDKHRDVNAKGRDGYTPLHYACEKGHFEIVKI-----------LTNHPQCNTEAEDVYDDRSL----------HKACEFGRLDIVRYLVIDKHRDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEAENNDNKWPLHLAIRSRSSYPSAKSHFTS 985          

HSP 3 Score: 186.422 bits (472), Expect = 2.650e-44
Identity = 138/494 (27.94%), Postives = 236/494 (47.77%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLIS-NKCDIEAENDQKMRPIHLACKYGNLDIVQ-LLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNNFSQYLIHNACRFNRIEIAKFLI-----------------------------------SNNCNLEAPNYKYDRPIHIACEYGFEDICS-LLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFK-VKCDWDSANYWNMRPIEYACKLGVKSIVQ-LLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNV--------RNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL-LDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLS 1396
            K+  L  AC +G++  V +L+  K  ++       Y P+H+A    +  IV++L + ++C++EAE     RP+H AC+ GN+DIV+ L+I+  CD++  D  G +P H ACE GH + +K L ++   ++EA++N++   +H AC    ++I + L+                                    + CNLEA     DRP+H ACE G  DI   L+I ++CDVNA++  G  P+H AC  GH   ++ L    +C+ ++ + WN RP+  AC+ G   IV+ L++D +C+ N                         K   G+     +PL+ +C++GH +IV+ + +    N         R L   C S   D+++   +ID  ++            H AC  GH  +V+ L +    + E ED+  DR +H AC F  +DIVR+L +D++   +   +    P+H      +  I+ +  +
Sbjct:  486 KDRPLHNACESGNVDIVRHLVIDKHCDVNAKGRIGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVINKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAKDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNA------------------------KGRNGY-----TPLHYACEKGHFEIVKFLTNHPQCNTKAEDVCDDRPLHKACKSRNVDIVR-YLVIDKHRDVNAKGRDGYTPLHYACEKGHFEIVKILTNHPQCNTEAEDVYDDRSLHKACEFGRLDIVRYLVIDKHRDVNAKGRNGYTPLHYACEKGHFEIVKILTN 949          

HSP 4 Score: 184.496 bits (467), Expect = 1.192e-43
Identity = 142/501 (28.34%), Postives = 235/501 (46.91%), Query Frame = 2
Query:   83 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISN-KCDIEAENDQKMRPIHLACKYGNLDIV-QLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNNFSQYLIHNACRFNRIEIAKFLI-----------------------------------SNNCNLEAPNYKYDRPIHIACEYGFEDIC-SLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFK-VKCDWDSANYWNMRPIEYACKLGVKSIVQ-LLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHK---EIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFL-LDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSR-NCSFDLSDEYK 1432
             E    L  AC   ++  V +L+  K  ++     N Y P+H+A       IV +L  + +C+IE E++   RP+H AC+ GN+DIV  L+I   C+++    +G +P H ACE GH +I+K L ++   ++E   +F+   +H AC    ++I + L+                                    + CNLEA     DRP+H ACE G  DI   L+I ++CDVNA+   G  P+H AC  GH  I++ L    +C+ ++ + WN RP+  AC+ G   IV+ L++D +C+ N   +  +     L Y+ +    +  K+   H +   E E      PL+ +C+ G++DIV  ++         +  +C  +A+D I    L          H AC  GH   V+ L +    +IE ED   +R +H AC   +VDIVR L +D++C  +   +    P+H      +  I+ +  +   C+ +  D  K
Sbjct:    8 FEDRRSLVYACKFRNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEEGCFEIVRILTDHPQCNIEVEDNSNDRPLHKACESGNVDIVCHLVIDKHCNVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEVEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGNFNDRPLHKACESGNVDIVHHLVIDKHCDVNAKGKDGYTPLHYACEKGHFEIVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTP---LHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLV---------INKHCDVNAKDRIGYTPL----------HYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEAEDYSK 486          

HSP 5 Score: 154.066 bits (388), Expect = 3.793e-34
Identity = 120/410 (29.27%), Postives = 214/410 (52.20%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLIS-NKCDIEAENDQKMRPIHLACKYGNLDIVQ-LLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNNFSQYLIHNACRFNRIEIAKFL-ISNNCNLEAPNYKYDRPIHIACEYG-FEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKC-DWDSANYWNMRPIEYACKLGVKSIVQLLLDN-NCNTNKTIYMEHKEVY---HLFYSKKTKRIDRFK-MHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIEC--------EDMSGDRLIHI 1252
            L +AC +G++  V +L+  K  ++  I  N Y P+H+A    +  IV++L + ++C++EAE     RP+H AC+ GN+DIV+ L+I   CD++  D  G +P H ACE GH + +K L ++   ++EA +N++   +H AC    ++I + L I  +C++ A       P+H ACE G FE +  L     C+  AE+   +RP+H AC + ++ I+ +L   K  D ++       P+ YAC+ G   IV++L ++  CNT      E ++VY    L  + +  R+D  + + I  H+++        +PL+ +C++GH +IV+++ +    N+    N+        I+ +S     K  F + +   S  +N+ Q+        ++C        EDMSG++ I I
Sbjct:  626 LHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKFLTNHPQCNTKAEDVCDDRPLHKACKSRNVDIVRYLVIDKHRDVNAKGRDGYTPLHYACEKGHFEIVKILTNHPQCNT------EAEDVYDDRSLHKACEFGRLDIVRYLVIDKHRDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEAENNDNKWPLHLAIRSRSSYPSAKSHFTSIVNYLSRVINMSQFSGIALLRVVKCILTGPPGAEDMSGNKRIEI 1029          
BLAST of SMED30018624 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 150.984 bits (380), Expect = 1.630e-36
Identity = 95/430 (22.09%), Postives = 190/430 (44.19%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +V  ++  K  N      N + P+H A   + V ++ELL+ +   ++A  +  + PIH+A   G+ +IV+ L  +G   +  ++ G +  H+A   G  D++++L+ NG+ ++ +    Q  +H A R  + EI + L+       A       P+H+A   G ++  SLL++    ++A    G  P+H+A   G +   + L + +   D+A    + P+  A     + +  LLLD+  + +      +  + H+   K    I    +  G       ++  SPL+++ Q G +D+V L+L          T N + +  +      L          H+A     + V + LL+ G ++++    +G   +H+AC + ++ +V FLL+     +   K    P+H      +  I+ + L    S
Sbjct:  383 KVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPL-HIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLL----------TKNANVNMGNKNGLTPL----------HLAAQEDRVGVAEVLLNHG-AEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRAS 790          

HSP 2 Score: 125.561 bits (314), Expect = 1.211e-28
Identity = 87/409 (21.27%), Postives = 177/409 (43.28%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKC--DIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG-----HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            LL +   N +  +++ + P+H A    N+ +  LL++     D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+  G+ + ++       +H A + + +   + L+ ++  ++     Y   +H+A   G   +  +++ +  + NA+   G  P+H+AC    ++++E L K      +     + PI  A  +G ++IV+ L  +  + N T  +  +   H+        + R+ +  G       K+ ++ L+++ + G  +IV+L+L               +SA        L          H+A   GH      LL+ G S +      G   +H+A  +  ++  + LL +    D   K    P+H+
Sbjct:  222 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARND--ITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPN-TTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQ----------KGAWASAATTSGYTPL----------HLAVREGHQETASLLLEHGAS-LSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHV 606          

HSP 3 Score: 122.479 bits (306), Expect = 9.370e-28
Identity = 108/465 (23.23%), Postives = 204/465 (43.87%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRP-IHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTI-----------YMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDL 1417
            +A   G+L + L+ L +   +I   N+N    +H A    +V +V  L+     ++A   +    +H+A   G  D+V+ L++NG +++    +G +P ++A +  HL++++FL+ +G+   +   + F+   +  A +    ++   L+ N+        K   P +HIA         +LL+Q + + + E+  G  P+H+A   G++ +   L       D     ++ P+  A K G  ++V+LLLD     +                 H++V  +        +DR    +   K   SPL+++ Q  HL+ V+L+L   +  V ++TN+     H +A     K   +I D+K          F   HIAC    + V++ LL  G S ++    SG   IH+A    H +IV+ L       +  N R +  +HM   +  A ++   L      D+
Sbjct:   49 RAARAGNLEKALDYL-KTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAV--ALQQGHDQVVSLLLENDT-----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEML-------LDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDV-PVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGAS-LQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDI 496          

HSP 4 Score: 107.071 bits (266), Expect = 5.053e-23
Identity = 64/257 (24.90%), Postives = 114/257 (44.36%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQL---LLDNNCNTNKTIYMEHK 892
            IE   LL +K    +   +    P+H A    N  +  LL+ +     A       P+H+A K   L+I   L+  G + +     G SP HLA + G +D++  L++  +++   N      +H A + +R+ +A+ L+++   ++A       P+H+AC YG   + S L++    +N++   G  P+H A   GH  II  L + +   +         +  A +LG  S+V     L D N      +  +HK
Sbjct:  580 IEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTLRGLTDENLTATPPVSEKHK 836          

HSP 5 Score: 91.6633 bits (226), Expect = 3.049e-18
Identity = 75/348 (21.55%), Postives = 139/348 (39.94%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            D N+    A   G+L+     +  G D+   N      +H A +   +E+   L+    +++A   K +  +HIA   G  D+   L+    +VNA++  G  P+++A    HL ++  L +       A      P+  A + G   +V LLL+N+      +        H+   K   +     +   H+ ++ES     PL+++   G++++  L+L+               +A D +    +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR        K    P+HM     + + + L L  +   D
Sbjct:   42 DSNASYLRAARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLN-------------RGAAVDFMARNDIT-------PLHVAAKRGNGNMVKLLLDRG-AKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVD 363          
BLAST of SMED30018624 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 150.984 bits (380), Expect = 1.982e-36
Identity = 95/430 (22.09%), Postives = 190/430 (44.19%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +V  ++  K  N      N + P+H A   + V ++ELL+ +   ++A  +  + PIH+A   G+ +IV+ L  +G   +  ++ G +  H+A   G  D++++L+ NG+ ++ +    Q  +H A R  + EI + L+       A       P+H+A   G ++  SLL++    ++A    G  P+H+A   G +   + L + +   D+A    + P+  A     + +  LLLD+  + +      +  + H+   K    I    +  G       ++  SPL+++ Q G +D+V L+L          T N + +  +      L          H+A     + V + LL+ G ++++    +G   +H+AC + ++ +V FLL+     +   K    P+H      +  I+ + L    S
Sbjct:  355 KVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPL-HIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLL----------TKNANVNMGNKNGLTPL----------HLAAQEDRVGVAEVLLNHG-AEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRAS 762          

HSP 2 Score: 119.013 bits (297), Expect = 1.388e-26
Identity = 86/407 (21.13%), Postives = 159/407 (39.07%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            I V  LL  +   ++ +  N   P+H A    N  +V+LL+     I+A+    + P+H   + G+  +V++L+  G  +     +G SP H+A +  HL+ ++ L+ +   ++   N     +H A      ++AK ++    N  A       P+HIAC+     +  LL++    + A    G  PIH+A   GH  I++ L       ++ N      +  A + G   +V+ LL N    +                                K+ ++ L+++ + G  +IV+L+L               +SA        L          H+A   GH      LL+ G S +      G   +H+A  +  ++  + LL +    D   K    P+H+
Sbjct:  222 INVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKA-----------------------------KDDQTALHIASRLGKAEIVQLLLQ----------KGAWASAATTSGYTPL----------HLAVREGHQETASLLLEHGAS-LSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHV 578          

HSP 3 Score: 115.931 bits (289), Expect = 1.035e-25
Identity = 88/412 (21.36%), Postives = 168/412 (40.78%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKC--DIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIG-WSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + P+H A    N+ +  LL++     D  A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+  G+ + ++       +H A + + +   + L+ ++  ++     Y   +H+A   G   +  +++ +  + NA+   G  P+H+AC    ++++E L K      +     + PI  A  +G ++IV+ L  +  + N T                             +   E+ L+++ + G  D+V  +L               + A+  IK +   DD+      HIA   G   +VQ LL  G W+       SG   +H+A    H +    LL+         K+   P+H+           L L +  + D +
Sbjct:  210 FTPLHIAAHYGNINVATLLLNRGAAVDFMARND--ITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTT-----------------------------NVRGETALHMAARAGQADVVRYLL--------------QNGAKVDIKAK---DDQT---ALHIASRLGKAEIVQLLLQKGAWA--SAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTA 568          

HSP 4 Score: 115.546 bits (288), Expect = 1.313e-25
Identity = 102/490 (20.82%), Postives = 207/490 (42.24%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISN--NCNLEAPNYKYDR------PIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            +A   G+L + L+ L +   +I   N+N    +H A    +V +V  L+     ++A   +    +H+A   G  D+V+ L++NG +++    +G +P ++A +  HL++++FL+ +G+   +   + F+   +                           H A R +  + A  L+ N  N ++E+  + Y        P+HIA  YG  ++ +LL+ +   V+        P+H+A   G+  +++ L       D+     + P+    + G + +V++LLD        +      +  L  + +   ++  ++ + H   ++       + L+V+   GH  + ++I+D +   N + L            N     E ++K  + +    E   T  H+A   GH N+V+ L   G S     ++ G+  +H+A      D+VR+LL      DI  K  Q  +H+      A I+ L L +  
Sbjct:   13 RAARAGNLEKALDYL-KTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGA---PILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASP-NTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGA 497          

HSP 5 Score: 107.457 bits (267), Expect = 4.447e-23
Identity = 64/257 (24.90%), Postives = 114/257 (44.36%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQL---LLDNNCNTNKTIYMEHK 892
            IE   LL +K    +   +    P+H A    N  +  LL+ +     A       P+H+A K   L+I   L+  G + +     G SP HLA + G +D++  L++  +++   N      +H A + +R+ +A+ L+++   ++A       P+H+AC YG   + S L++    +N++   G  P+H A   GH  II  L + +   +         +  A +LG  S+V     L D N      +  +HK
Sbjct:  552 IEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTLRGLTDENLTATPPVSEKHK 808          

HSP 6 Score: 87.8113 bits (216), Expect = 3.995e-17
Identity = 73/348 (20.98%), Postives = 136/348 (39.08%), Query Frame = 2
Query:  410 ACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            A   G+L+     +  G D+   N      +H A +   +E+   L+    +++A   K +  +HIA   G  D+   L+    +VNA++  G  P+++A    HL ++  L +       A      P+  A + G   +V LLL+N+      +        H+   K   +     +   H+ ++ES             PL+++   G++++  L+L+               +A D +    +          H+A   G+ N+V+ LLD G + I+ +   G   +H      H  +V  LLDR        K    P+HM     + + + L L  +   D
Sbjct:   14 AARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLN-------------RGAAVDFMARNDIT-------PLHVAAKRGNGNMVKLLLDRG-AKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVD 335          
BLAST of SMED30018624 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 149.058 bits (375), Expect = 6.014e-36
Identity = 110/478 (23.01%), Postives = 200/478 (41.84%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRI----DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMI-----KCQSLIDDEKERFCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            + V  LL  +  N+    +N   P+H A    NV +V LL+     I+A+   ++ P+H A + G++ I+++L+  G  +     +G SP H+A +  H+D                                 + K L+  G+    R  N F+   +H AC+ N + +   L+ ++ +LEA       P+H+A   G  +I  +L+Q+    NA N     P+H+A   GH  + E L +     D+    +   +  A ++G K +V+LLL++  N N T    H  ++       T+ +    D         K+  +PL+V+ + G +D+ EL+L+       A    +  L    H +  D++     K  S     +  +   HIA     + V   LL  G S    E + G   +H+A      D+V  L+ +    ++ NK    P+H++    +  I  + + +  S
Sbjct:  191 LNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTP--LHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGAS-ANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGAS 665          

HSP 2 Score: 136.732 bits (343), Expect = 4.119e-32
Identity = 95/421 (22.57%), Postives = 185/421 (43.94%), Query Frame = 2
Query:  179 INENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            I    + P+H A    N+ + +LL++   ++       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ II+ L+  G+ ++A+       IH A + + ++  + L+  N  ++     +  P+H+A   G   +  +L+ +    N     G  P+H+AC   HLR+++ L K     ++     + P+  A  +G  +IV++LL    + N +       ++M     H EV   F  +    +D         K+ ++ L+ + + GH ++V+L+L+ +  N  + T   H+                     HIA   GH   V+ LLD+     +     G   +H+A  +  VD+   LL+R    +   K    P+H+    +   ++ L +S+  S
Sbjct:  174 ITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAE-FLLQNAAPVD------AKAKDDQTSLHCAARMGHKELVKLLLEHKA-NPNSTTTAGHTP-------------------LHIAAREGHTQTVRILLDMNAQQTKMTK-KGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGS 566          

HSP 3 Score: 135.961 bits (341), Expect = 6.161e-32
Identity = 102/465 (21.94%), Postives = 190/465 (40.86%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK-----------TIYMEHKEVYHLFYSK-----KTKRIDRFKMHIGHHK-EIE--------------------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            GD ++ +  L +    I++I  +   P+H A    +  + ++L+               P+H+ACK  +L ++ LL+ +   L      G +P H+A   GHL+I+K L+  G+   A N   +  +H A R    E+A+FL+ N   ++A        +H A   G +++  LL++   + N+  + G+ P+H+A   GH + +  L  +             P+  A K G   + +LLL+   N N             ++  + +V +L  SK        R     +HI   + ++E                    +PL+++ Q G  D+V L++ ++  NV NL N    +                    H+    GH+ +   L+  G S      M G   +H+AC + ++ +V+FLL +    +   +    P+H      +  I+ L L    
Sbjct:  287 GDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLI-SKQANV-NLGNKSGLTP------------------LHLVAQEGHVGIADILVKQGASVYAATRM-GYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGA 730          

HSP 4 Score: 117.857 bits (294), Expect = 2.624e-26
Identity = 100/458 (21.83%), Postives = 189/458 (41.27%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD-------------LEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +A  +G+L + L+ + +   +I   N+N    +H A    +V +V  L+ N   +E    +    +H+A   G   +V  L++ G +++     G +P ++A +  HL+++KFL+ NG++             L    +F  YL  NA       +  F+ +              P+HIA  Y   ++  LL+ +  +VN     G  P+H+A   G++ ++  L       D+     + P+  A + G   I+++LLD               ++    + +   +D  +  + ++ EI+       +PL+V+   GH  + +++LD   + N R L                        F   HIAC   HL V+  LL    S +E    SG   +H+A    H++IV+ LL +    +  N + + P+HM   + +  +    L      D
Sbjct:   16 RAARSGNLDKALDHI-KNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFITATGFT----------PLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIH---MAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNG----------------------FTPLHIACKKNHLRVMDLLLKHSAS-LEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVD 436          

HSP 5 Score: 111.309 bits (277), Expect = 2.370e-24
Identity = 58/205 (28.29%), Postives = 100/205 (48.78%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHL 745
            ++V+NLL  K  +      N Y P+H A   + V +   L+       AE+ Q + P+HLA + G  D+V LLIS   ++++ +  G +P HL  + GH+ I   L+  G+ + A        +H AC +  I++ KFL+    N+         P+H A + G  DI +LL++     N   + G   + +A   G++ +I+ L
Sbjct:  554 LDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSALAIAKRLGYISVIDVL 758          

HSP 6 Score: 103.605 bits (257), Expect = 5.724e-22
Identity = 92/417 (22.06%), Postives = 175/417 (41.97%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGF-------------EDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            I N  DI   N   +  +HLA K G++ +V  L+ NG  L      GN+  H+A   G   ++  L++ G+++ A++      ++ A + N +E+ KFL+ N  N   P       + +A  Y F             +++CS +           + G  P+H+A    +L + + L     + +      + P+  A + G   +V+LLLD     +                 KTK  D             +PL+ + + GH+ I+E++LD         +N  +  H +A+         +++  + IDD      T  H+A   GH  + + LLD G +      ++G   +HIAC  +H+ ++  LL  +   + + +    P+H+     + +I+ + L +  S + S+
Sbjct:   30 IKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEVKPSAVSLANSYSFPLYLSTNASHITLKNVCSFI----------TATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDA----------------KTK--DEL-----------TPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKG-AKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASN 406          
BLAST of SMED30018624 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 144.821 bits (364), Expect = 1.196e-34
Identity = 115/487 (23.61%), Postives = 204/487 (41.89%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKK---TKRI--DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNK-RYQ----KPIHMIPSSSYASILPLFLSRNCSF 1411
            + V  LL  +  N+    +N   P+H A    NV +V LL+     I+A    ++ P+H A + G++ ++++L+  G  +     +G SP H+A +  H+D                                 + K L+  G+   AR  N F+   +H AC+ N +     L+ ++ +LEA       P+H+A   G  +I   L+Q+    NA N     P+H+A   GH  + + L +     D+    +  P+  A ++G K +V+LLL++  N +      H  + H+   +    T RI  D         K+  +PL+V+C+ G +D+ EL+L+       A    +  L    H +  D++K   L+        T        HIA     L V   LL  G ++   E + G   +H+A      D+V  L+ +    ++ NK R Q     P+H++    +  I  + + +  S 
Sbjct:  194 LSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTP--LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPL-HIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKL--LVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLLQCG-ANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASV 674          

HSP 2 Score: 142.895 bits (359), Expect = 4.645e-34
Identity = 102/456 (22.37%), Postives = 204/456 (44.74%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS--FDLSDEYKSINSYKKSEKLEKA 1474
            +L +   N + +++  + P+H A    N+++ +LL++   ++       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ +I+ L+  G+ ++A+       IH A + + ++  + L+  N  ++     +  P+H+A   G   +  +L+ +    NA    G  P+H+AC   H+R ++ L K     ++     + P+  A  +G  +IV+ LL    + N +       ++M     H EV   F  +   ++D         K+ ++PL+ + + GH ++V+L+L+ +  N  + T   H+                     HIA   GH +  + LLD G +        G   +H+AC +  VD+   LL+R    +   K    P+H+    +   ++ L +S+  S      + Y  ++   K  +LE A
Sbjct:  166 VLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQ-FLLQNLAQVD------AKAKDDQTPLHCAARMGHKELVKLLLEHKA-NPDSATTAGHTP-------------------LHIAAREGHAHTTRILLD-GNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVA 593          

HSP 3 Score: 140.584 bits (353), Expect = 2.158e-33
Identity = 92/411 (22.38%), Postives = 182/411 (44.28%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            N + P+H A   +++  ++LL+ +   +EA  +  + P+H+A   G+L+IV+ L+  G   + +++   +P H+A   GH ++ +FL+ N + ++A+    Q  +H A R    E+ K L+ +  N ++       P+HIA   G      +L+  N         G  P+H+AC  G + + E L +   + ++A    + P+  A       +V+LL+    + + T    +  + H+   +    +    +  G +   ES     PL+++ Q G  D+V L++ ++  NV NL N   +    +                H+    GH+ +   L+  G S      M G   +H+AC + ++ +V+FLL +    +   +    P+H      +  I+ L L    
Sbjct:  345 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPL-HIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLI-SKQGNV-NLGNKVRTQLTALTPL-------------HLVAQEGHVGIADMLVKQGASVYSASRM-GYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGA 738          

HSP 4 Score: 117.472 bits (293), Expect = 3.485e-26
Identity = 103/465 (22.15%), Postives = 195/465 (41.94%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            I   N+N    +H A    +V +V  L+ +  D+E    +    +H+A   G   +V  L+  G +++     G SP ++A +  HL+++KFL+ NG++  L   + F+   +                           H A R +    A  L+ N+ N +  +     P+HIA  Y    +  LL+ +  +VN     G  P+H+A   G++ ++  L       D+     + P+  A + G   ++++LLD        I  + K  +  +  + +   +D  +  + ++ EI+       +PL+V+   GH  + +++LD   + N R L            N   S + ++K  + ++   E   T  H+A   GHLN+V+ LL  G S     ++  +  +H+A    H ++ +FLL      D   K  Q P+H      +  ++ L L    + D
Sbjct:   13 INTANQNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGA----PIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASP-NASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPD 472          

HSP 5 Score: 116.316 bits (290), Expect = 8.253e-26
Identity = 94/417 (22.54%), Postives = 181/417 (43.41%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNR------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAED--------MIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            I N   I   N   +  +HLA K G++ +V  L+ +G DL      GN+  H+A   G   ++  L+  G+++ A++      ++ A + N +E+ KFL+ N  N   P      P+ +A + G E++ +LLI          +YG +       +H+A  N   R    L +   + D  +     P+  A      S+ QLLL+   N N   +     +  L  + +   +   ++ +    +I+       +PL+ + + GH+ ++E++LD         +N  +  H +A+         +++  + IDD      T  H+A   GH  + + LLD G +      ++G   +HIAC  +H+  +  LL  +   + + +    P+H+     + +I+   L R  S + S+
Sbjct:    7 IKNGIAINTANQNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLI----------NYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVN---FTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKG-AKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASN 409          

HSP 6 Score: 104.375 bits (259), Expect = 3.165e-22
Identity = 65/250 (26.00%), Postives = 112/250 (44.80%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYL-----IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            AC  G  ++V  LL  +  N     +N   P+H A+ ++N+ +V+LL+S      +       P+H+A K   L++   L+  G + +   L G +P HLA + G  D++  LIS   ++   N     L     +H   +   + IA  L+    ++ + +      +H+AC YG   +   L+QQ   VN +   G  P+H A   GH  I+  L K     +        P+  A +LG  S++ +L
Sbjct:  518 ACKYGK-VDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 766          

HSP 7 Score: 102.449 bits (254), Expect = 1.335e-21
Identity = 68/292 (23.29%), Postives = 123/292 (42.12%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE--ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENS--------------------------------------YGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL-----LDNNCNTNKT 874
            E++ LL     N +      + P+H A    +     +L+           +   P+H+ACKYG +D+ +LL+  G + +    +G +P H+A    +LD++K L+S G      ARN ++   +H A + N++E+A  L+    N  + + +   P+H+A + G  D+ +LLI +  +VN  N                                        G   +H+AC  G++++++ L + +   +        P+  A + G   IV LL     L N   TN T
Sbjct:  459 ELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTP--LHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGT 748          
BLAST of SMED30018624 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 140.584 bits (353), Expect = 2.709e-33
Identity = 90/393 (22.90%), Postives = 184/393 (46.82%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLT-NNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLL 1294
            +V  ++  K  N      N + P+H A   + + ++ELL+ +   I+A  +  + PIH+A   G+ +IV  L+++G   +  ++ G +  H+A   G  +++K+L+ NG+ ++A++   Q  +H + R  + EI + L+ +    ++       P+H+A   G +D+ S+L+ Q   +      G  P+H+A   G + +   L + +   D++    + P+  A     + +  LLLD   + +      +  + H+   K    I    +  G       ++  SPL+++ Q G++D+V L++       R+ T N C+ S    +               H+A     +NV + L++ G + ++ E   G   +H+AC + +V +V FLL
Sbjct:  328 KVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPL-HIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLM------ARDATINLCNKSGLTPL---------------HLAAQEDQVNVAEVLVNHGAT-VDPETKMGYTPLHVACHYGNVKMVHFLL 697          

HSP 2 Score: 129.798 bits (325), Expect = 6.621e-30
Identity = 91/435 (20.92%), Postives = 187/435 (42.99%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKC--DIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            D  +   LL +   N +  +++ + P+H A    N+ +  LL++     D +A ND  + P+H+A K GN ++V+LL+  G  +     DG +P H     GH  +++ L+  G+ + ++       +H A + + +   + L+ +   ++     Y   +H+A   G   +  +++ +  + NA+   G  P+H+AC    ++++E L K      +     + PI  A  +G ++IV  L+++  + N T  +  +   H+        + ++ +  G +     K+ ++PL++S + G  +I++ +L              H +  D                 H+A   GH +V   LLD G S +      G   +H+A  +  +++   LL +    D   K    P+H+        +  L L +  S
Sbjct:  160 DDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARND--ITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPN-TTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQ-------------HGACPDSTTTSGYTP-------LHLAAREGHKDVASILLDQGAS-LGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGAS 570          

HSP 3 Score: 125.946 bits (315), Expect = 9.163e-29
Identity = 100/441 (22.68%), Postives = 194/441 (43.99%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNN----CHSSAE-DMIKCQSLIDDEKER--------FCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSF 1411
            +EV+  L +    ++   +     +H A       +V+ L++N  ++ A++     P+++A +  +LD+V+ L+ NG   S+   DG +P  +A + GH  ++  L+ N    + +       +H A R +  + A  L+ N+ N +  +     P+HIA  YG  ++ +LL+ +   V+ +      P+H+A   G+  +++ L +     D+     + P+    + G + +V++LLD                     SK    +              SPL+++ Q  HL+ V+L+L   +  V ++TN+     H +A     K   +I D+K          F   HIAC    + V++ LL  G S I+    SG   IH+A    H +IV  L++     +  N R +  +HM   +    ++  +L +N ++
Sbjct:   34 VEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAP---------------ILSKTKNGL--------------SPLHMATQGDHLNCVQLLLHHEV-PVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGAS-IQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVK-YLVQNGAY 438          

HSP 4 Score: 109.768 bits (273), Expect = 8.277e-24
Identity = 92/441 (20.86%), Postives = 183/441 (41.50%), Query Frame = 2
Query:  182 NENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSSAED--------MIKCQSLID--DEKERFCTHIACSSGHLNVVQYLLDIGWSDIECED---MSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
             E+ + P+  A+   +  +V LL+ N    + +   ++  +H+A +  +     LL+ N  +  V    G +P H+A  +G++++   L++ G+  D +ARN+ +   +H A +     + K L+     ++A       P+H     G E +  +L+ +   + ++   G  P+H+A    HL  ++ L   +   D      +  +  A   G   + ++++D   N N            L  + K  RI   ++ + H   I+       +P++V+   GH +IV  +++        NVR  T   H +A          +++  + +D   + ++   HI+   G   ++Q LL  G     C D    SG   +H+A    H D+   LLD+     I  K+   P+H+        +  L L +    D S +
Sbjct:  117 TEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITP--LHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKAL---NGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGET-ALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHG----ACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGK 543          

HSP 5 Score: 98.9821 bits (245), Expect = 1.890e-20
Identity = 55/237 (23.21%), Postives = 99/237 (41.77%), Query Frame = 2
Query:  194 YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYH 904
            Y P+H A    +  +  +L+     +     +   P+H+A KYG +++  LL+        +   G +P H+A  + +  +   L+  G+   A        +H A + N++EIA  L+    +  A   +   P+H+A + G  D+ +LL+ ++  +N  N  G  P+HLA     + + E L       D        P+  AC  G   +V  LL N    N    +   +  H
Sbjct:  480 YTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKVTAAQQGH 716          

HSP 6 Score: 88.1965 bits (217), Expect = 3.313e-17
Identity = 74/319 (23.20%), Postives = 136/319 (42.63%), Query Frame = 2
Query:  449 ISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELIL------DARLRN-VRNLTNNCHSSAEDMIKC----QSLIDDEKERFCTHIACS--SGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            + +G D+   N      +H A +   +E+   LI     ++A   K +  +HIA   G  D+   L+    +VNA++  G  P+++A    HL ++ +L         A      P+  A + G   +V LLL+N+      +        H+   K   +     +   H+ ++ES     PL+++   G++++  L+L      D + RN +  L         +M+K      + ID + +   T + C   SGH  VV+ LLD G + I  +  +G   +H+A    H++ V+ LL      D +   Y   +H+
Sbjct:    8 LKSGVDINICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRL-----PALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRG-APILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHV 320          

HSP 7 Score: 87.8113 bits (216), Expect = 4.027e-17
Identity = 58/238 (24.37%), Postives = 103/238 (43.28%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            IEV NLL +K    +   ++   P+H A    N  +  LL+       A       P+H+A K   ++I   L+  G D +     G SP HLA + G++D++  L++  + +   N      +H A + +++ +A+ L+++   ++        P+H+AC YG   +   L++    VNA+          A   GH  II  L +     +         +  A +LG  S+V  L
Sbjct:  525 IEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKV------TAAQQGHTHIINLLLQHGAAPNELTVNGNTALSIARRLGYISVVDTL 756          

HSP 8 Score: 87.8113 bits (216), Expect = 4.310e-17
Identity = 51/246 (20.73%), Postives = 108/246 (43.90%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNK 871
            +V ++L  +  ++    +  + P+H A     + +  LL+  +   +A     + P+H+A  Y N  +  LL+  G        +G +P H+A +   ++I   L+  G+D  A        +H A +   +++   L++ +  +   N     P+H+A +    ++  +L+     V+ E   G  P+H+AC  G+++++  L K +         N +    A + G   I+ LLL +    N+
Sbjct:  493 DVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAK------VNAKTKVTAAQQGHTHIINLLLQHGAAPNE 732          
BLAST of SMED30018624 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 149.058 bits (375), Expect = 6.698e-37
Identity = 93/392 (23.72%), Postives = 174/392 (44.39%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDAR----LRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCC 1309
            N + P+H A   + + ++ELL+ +   I    +  + PIH+A   G+L++VQLL+ NG   +  ++ G +P H+A   G L+++K L+ +G+ +  +    Q  +H A R  + EI + L+    +  AP      P++ A   G  D+ SLL++   D++     G  P+H+A   G L +   L +     D      + P+  A     + +  LLLD   + + T    +  + H+   K    + +  + +G       ++  +PL+++ Q GH D+V L+L       L     LT    ++ ED +    ++   K    T         H+A   G++ +V +LL    + +  +  +G   +H A    H  +V  LL     
Sbjct:  376 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPL-HIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLR-NRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEAS 765          

HSP 2 Score: 134.806 bits (338), Expect = 1.778e-32
Identity = 111/469 (23.67%), Postives = 201/469 (42.86%), Query Frame = 2
Query:  170 IEEINENLYRPIHFAI--TNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYMEHK----EVYHLFYSKKTK-----RIDRFKMHIGHH---KEI------------------ESPLYVSCQRGHLDIVELILD--ARLRNV--RNLTNNCHSSAEDMIKCQSLI-------DDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            + +  E+ + P+H A    N NVA + L +    D  A N   + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH  +I+ L+   + + A+       +H A +   +   + L+     ++     Y  P+H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  ++VQLLL N  ++N T       ++M  +    EV         +     R D+  +HI       EI                   +PLY + + GH D+  L+L+  A +  V  +  T    +S    ++  +L+       D + +   T  H+A    H  V   LLD G S       +G   +HIA   + +++ + LL+     +++ ++   P+H+     +A ++ L L R+ S  L+
Sbjct:  205 LNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNG--ITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASP-HSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLA 670          

HSP 3 Score: 124.02 bits (310), Expect = 4.157e-29
Identity = 108/490 (22.04%), Postives = 206/490 (42.04%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCN--------LEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD-ARLRNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            +A  NG+L  +++ L +   +I   N+N    +H A    +V +V  L+  + +I+         +H+A   G  DIV++L+  G +++    +G +P ++A +  HL+I+KFL+ NG++  +   + F+   +                           H A R +    A  L+ +  N        L         P+HIA  YG  ++ +LL+     V+     G  P+H+A   G+  +++ L       D+     + P+  A + G + ++++LL+ +      +      +  L  + + + +   ++ +G    ++       +PL+V+   GH  + +L+LD     N R L            N     E ++K  + I    E   T  H+A   GHLNVVQ LL  G S     ++ G+  +H+A     +++V+ LL       +  +  Q P+H+      A I+ L L R  
Sbjct:   16 RAARNGNLERLMDHL-KGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDA---PILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASS-NATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGS 500          

HSP 4 Score: 123.635 bits (309), Expect = 5.721e-29
Identity = 88/410 (21.46%), Postives = 174/410 (42.44%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN----TNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            +V+ +L  +   I    +N   P+H A    ++A V+LL+  +  ++      + P+H+A   G+  + +LL+  G + +   L+G +P H+AC+   + +++ L+ +G+ +          IH A     + + + L+ N  +  A N + + P+H+A   G  ++   L+Q    V  +      P+H+A   G   I++ L +   D ++       P+ +A + G   +  LLL+N  +    T K     H    +      T  ++++       K   +PL+V+    H  +  L+LD           + HS+A++                 HIA     L V + LL++G ++       G   +H+A    H D+V  LL R+    +  K     +H+
Sbjct:  292 KVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDK--------GASPHSTAKNGYT------------PLHIAAKKNQLEVGKVLLELG-AETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHL 680          

HSP 5 Score: 96.6709 bits (239), Expect = 1.108e-20
Identity = 69/302 (22.85%), Postives = 129/302 (42.72%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE--ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            E++ LL ++ ++      +   P++FA    +  +  LL+ N  DI     +   P+H+A KYG L++  LL+           +G +P H+A  + H  +   L+  G+     A+N ++   +H A + N++E+ K L+           +   P+H+A + G  D+ +LL+ ++  ++     G   +HLA     + + E L K K D ++       P+  A   G   +V  LL N    N                 KT+         G+     +PL+ + Q+GH  +V  +L
Sbjct:  490 EIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTP--LHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVN----------------AKTRN--------GY-----TPLHQAAQQGHTHVVNALL 760          

HSP 6 Score: 88.1965 bits (217), Expect = 5.345e-18
Identity = 53/238 (22.27%), Postives = 105/238 (44.12%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV  LL  K  + +   +N   P+H A    +  +  LL+       +       P+H+A K   L++ ++L+  G + ++    G +P HLA + GH D++  L+   + +          +H A + +++ +A+ L  +  ++         P+H+A  YG   + + L++    VNA+   G  P+H A   GH  ++  L + +   +         +  A +LG  S+V  L
Sbjct:  555 LEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 792          

HSP 7 Score: 65.855 bits (159), Expect = 3.775e-11
Identity = 61/294 (20.75%), Postives = 113/294 (38.44%), Query Frame = 2
Query:  554 NNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGW-SDIECEDM------SGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            N CN    N      +H+A + G  D+ + L+++  +++     GN  +H+A + G   I+  L +   + ++ +     P+  A +     IV+ LLDN  N  +++  E                D F           +PL V+ Q+GH  +V L+L+              S  +  ++  +L          HIA      N    LL     +D+  + M      SG   +HIA  + ++++   LL+     D   +    P+H+       +++ L L R    D
Sbjct:   37 NTCNQNGLNA-----LHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGAN--QSVATE----------------DGF-----------TPLAVALQQGHEQVVGLLLE--------------SDTKGKVRLPAL----------HIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKID 272          
BLAST of SMED30018624 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 149.058 bits (375), Expect = 7.584e-37
Identity = 94/388 (24.23%), Postives = 181/388 (46.65%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSS------------AEDMIKCQSLIDDEKERFCTH--IACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLL 1294
            N + P+H A   + + ++ELL+     IEA  +  + P+H++   GNL+IV +L+  G   +  ++ G +  H+A   G  ++++ L+ NG+ ++A+    Q  +H A R  ++EI + L+ +  + +A       P+H+A   G ED+ ++L++    +      G  P+H+A   G + +   L +     D+A    + P+  A     + +  LLLD   + + T    +  + H+   K    I    +  G       ++  +PL++  Q GH D+  L+L AR  +V + T +  S             AE ++K  + ++   +   T   +AC  G++ +V +LL  G +D+  +  +G   +H A    H  IV  LL
Sbjct:   14 NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPL-HIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLL-ARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHG-ADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398          

HSP 2 Score: 115.161 bits (287), Expect = 2.845e-26
Identity = 89/399 (22.31%), Postives = 170/399 (42.61%), Query Frame = 2
Query:   83 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG----HHKEIESPLYVSCQRGHLDIVELIL--DARLRNVRNLTNNCHSSAEDMIKCQSLIDDEK----ERFCTHIACSSGHLNVVQYLLDI-GWSDIECED-MSGDRL 1243
            +  E  L  A   G   EV+  L R    ++        P+H A     + IV+LL+ +    +A       P+H+A + G+ D+  +L+ +G  L+V    G +P H+A ++G +++   L+   +  +A        +H A  ++  ++A  L+    +  A       P+HIA +    +I + L++   + NA    G  P+HL    GH  +   L     D + A    + P+  A +    ++ ++L+ +  + N T  + +  +    +    K ++    H        K   +PL+ + Q+GH  IV L+L   AR  +V  +  N   +    +   S++D  K    E   +    +    NV + + ++   SD E ED M+GD +
Sbjct:   79 VRGETALHMAARAGQ-TEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDV-TVNGNTALAIARRLGYISVVDTLKVVTEETITSTTVSNPSSRNVPETMTEVLEVSDDEGEDTMTGDGM 475          

HSP 3 Score: 85.5001 bits (210), Expect = 3.418e-17
Identity = 73/370 (19.73%), Postives = 144/370 (38.92%), Query Frame = 2
Query:  347 LISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKS 1456
            L+  G + +V  L+G +P H+AC+   + +++ L+  G+ +EA        +H +     + I   L+    +  A N + +  +H+A   G  ++   L++    V+A+      P+H+A   G L I++ L +     D+A      P+  A + G + +  +LL++  +   T                              K+  +PL+V+ + G +++  L+L                ++ D      L          H+A    +  V   LLD G S       +G   +HIA     ++I   LL+     + + ++   P+H+     +A +  L L+R         Y  +N   KS
Sbjct:    1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTT-----------------------------KKGFTPLHVASKYGKMEVASLLLQ-------------RFASPDAAGKNGLTP-------LHVASHYDNQKVALLLLDQGASP-HATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR---------YADVNHATKS 311          

HSP 4 Score: 72.7886 bits (177), Expect = 2.771e-13
Identity = 60/284 (21.13%), Postives = 114/284 (40.14%), Query Frame = 2
Query:  644 LIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS--DEYKSINSYKKSEKLEKA 1474
            L+ +  + N     G  P+H+AC    +R++E L K     ++     + P+  +  +G  +IV +LL    + N T  +  +   H+        + R  +  G       +E ++PL+++ + G L+IV+L+L              H ++ D                 H+A   GH +V   LL+ G S +      G   +H+A  +  +++   LL R    D   K    P+H+        +  L L +  S   +  + Y  ++   K ++LE A
Sbjct:    1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNAT-NVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQ-------------HMASPDAATSNGYTP-------LHVAAREGHEDVTAILLEHGAS-LAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIA 262          
BLAST of SMED30018624 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 148.673 bits (374), Expect = 1.270e-36
Identity = 93/407 (22.85%), Postives = 180/407 (44.23%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            N + P+H A   + + ++ELL+     IEA  +  + P+H++   GNL+IV +L+  G   +  ++ G +  H+A   G  ++++ L+ NG+ ++A+    Q  +H A R  ++EI + L+ +  + +A       P+H+A   G ED+ ++L++    +      G  P+H+A   G + +   L +     D+A    + P+  A     + +  LLLD   + + T    +  + H+   K    I    +  G       ++  +PL++  Q GH D+  L+L AR  +V + T +  S                     H+A     +NV + L+  G + +      G   + +AC + ++ +V FLL      +   K    P+H      +  I+ L L
Sbjct:  104 NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPL-HIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLL-ARYADVNHATKSGLSP-------------------LHLAAQEDKVNVAEILVKHG-AHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 488          

HSP 2 Score: 117.472 bits (293), Expect = 6.801e-27
Identity = 90/397 (22.67%), Postives = 172/397 (43.32%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIG----HHKEIESPLYVSCQRGHLDIVELIL--DARLRNVRNLTNNCHSSAEDMIKCQSLID-----DEKERFCTHIACSSGHLNVVQYLLDI-GWSDIECED-MSGDRL 1243
            E  L  A   G   EV+  L R    ++        P+H A     + IV+LL+ +    +A       P+H+A + G+ D+  +L+ +G  L+V    G +P H+A ++G +++   L+   +  +A        +H A  ++  ++A  L+    +  A       P+HIA +    +I + L++   + NA    G  P+HL    GH  +   L     D + A    + P+  A +    ++ ++L+ +  + N T  + +  +    +    K ++    H        K   +PL+ + Q+GH  IV L+L   AR  +V  +  N   +    +   S++D      E+    T ++  S  +NV + + ++   SD E ED M+GD +
Sbjct:  172 ETALHMAARAGQ-TEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDV-TVNGNTALAIARRLGYISVVDTLKVVTEETITSTTVSNPSSRVNVPETMTEVLEVSDDEGEDTMTGDGM 566          

HSP 3 Score: 117.087 bits (292), Expect = 9.327e-27
Identity = 96/406 (23.65%), Postives = 175/406 (43.10%), Query Frame = 2
Query:  200 PIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS--DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-----SPLYVSCQRGHLDIVELIL------DARLRNVRNLTNNCHSSAEDMIKCQSLIDDE--------KERFCT-HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            P+H A  + +  +VE+L+     I A+    + P+H+A +  +++ VQ+L+     +    LD  +  H+A   GH    K L+  G+  ++ A N F+   +H AC+ NRI + + L+    ++EA       P+H++   G  +I  +L+Q     NA N  G   +H+A   G   ++  L +     D+       P+  A +LG   IVQLLL +  + +      +  + H+   +  + +    +  G    +      +PL+V+ + G +++  L+L      DA  +N   LT    +S  D  K   L+ D+        K  +   HIA     L +   LL+ G ++       G   +H+     H D+   LL R    +   K    P+H+
Sbjct:    9 PLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTP--LHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPL-HVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKN--GLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYG-AETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHL 408          

HSP 4 Score: 102.834 bits (255), Expect = 2.128e-22
Identity = 78/370 (21.08%), Postives = 151/370 (40.81%), Query Frame = 2
Query:  386 DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS--DEYKSINSYKKSEKLEKA 1474
            DG +P H A   GH  +++ L+  G+ + A+       +H A + + +E  + L+     ++     Y   +H+A   G      +L+ +  + N     G  P+H+AC    +R++E L K     ++     + P+  +  +G  +IV +LL    + N T  +  +   H+        + R  +  G       +E ++PL+++ + G L+IV+L+L              H ++ D                 H+A   GH +V   LL+ G S +      G   +H+A  +  +++   LL R    D   K    P+H+        +  L L +  S   +  + Y  ++   K ++LE A
Sbjct:    5 DGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNAT-NVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQ-------------HMASPDAATSNGYTP-------LHVAAREGHEDVTAILLEHGAS-LAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIA 352          
BLAST of SMED30018624 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 147.132 bits (370), Expect = 1.756e-36
Identity = 103/437 (23.57%), Postives = 196/437 (44.85%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            ++ LL  +   I+    +   P+H A  + +  ++E+L+     I A+    + P+H+A +  +L  VQLL+     +    LD  +P H+A   GH  + K L+  G +  AR  N F+   +H AC+ NRI++ + L+ +  ++         PIH+A   G  ++  LL+Q     NA N  G  P+H+A   G L +++ L +           +  P+  A +LG   IVQLLL    + N         +Y   ++ +    D   + + +  +I        +PL+++ + G L++  L+L+             ++SA+  +  Q+ +         H+A    H  V   LLD G S       +G   +HIA   + +++ + LL+     +++ ++   P+H+     +A ++ L L R+ S  L+
Sbjct:  253 MVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTP--LHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLY---FASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEK------------YASADPKVAGQNGLTP------LHVATHYDHQKVALLLLDKGASP-HSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLA 665          

HSP 2 Score: 131.724 bits (330), Expect = 1.474e-31
Identity = 90/410 (21.95%), Postives = 170/410 (41.46%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHM 1351
            G+ +  + LL  +   ++++  +   P+H A    +  + +LL+    +  A       P+H+ACK   + +++LL+ +G  +      G +P H+A   GHL++++ L+ NG+   A N   +  +H A R  ++E+ K L+ +   +     +   P+HIA   G  +I  LL+Q+  D NA  + G+ P++ A   GH  +   L +   D          P+  A K G   +  LLL+   + +  +  ++                             +PL+V+    H  +  L+LD           + HS+A++                 HIA     L V + LL++G ++       G   +H+A    H D+V  LL R+    +  K     +H+
Sbjct:  314 GEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNG---------------------------LTPLHVATHYDHQKVALLLLDK--------GASPHSTAKNGYT------------PLHIAAKKNQLEVGKVLLELG-AETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHL 675          

HSP 3 Score: 130.568 bits (327), Expect = 3.166e-31
Identity = 107/482 (22.20%), Postives = 207/482 (42.95%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD-ARLRNVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            +A  NG+L  +++ L +   +I   N+N    +H A    +V +V  L+  + +I+         +H+A   G  DIV++L+  G +++    +G +P ++A +  HL+I+KFL+ NG++  +   + F+   +                           H A R +    A  L+ +  N +  +     P+HIA  YG  ++ +LL+     V+     G  P+H+A   G+  +++ L       D+     + P+  A + G + ++++LL+ +      +      +  L  + + + +   ++ +G    ++       +PL+V+   GH  + +L+LD     N R L            N     E ++K  + I    E   T  H+A   GHLNVVQ LL  G S     ++ G+  +H+A     +++V+ LL       +  +  Q P+H+      A I+ L L R  
Sbjct:   17 RAARNGNLERLMDHL-KGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDA---PILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASS-NATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGS 493          

HSP 4 Score: 100.138 bits (248), Expect = 8.946e-22
Identity = 77/373 (20.64%), Postives = 152/373 (40.75%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQ----NCDVNAENSY-------------------------------GNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-------KTIYMEHKE----VYHLFYSKK-----TKRIDRFKMHIGHH---------------------KEIESPLYVSCQRGHLDIVELIL 1033
            I+V+ LL +   +I    E+   PIH A    ++ +V+LL+ N     A N +   P+H+A + G L++V+ L+ +G  + V   +  +P H+A   G  +I++ L+  GSD  A        ++ A R    ++A  L+ N  ++     K   P+HIA +YG  ++ +LL+++    +  V  +N                                 G  P+H+A     L + + L ++  + +      + P+  A + G   +V LLL  + + +         +++  +E    V  +    K     T ++    +H+  H                     +   +PL+ + Q+GH  +V  +L
Sbjct:  383 IKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755          

HSP 5 Score: 84.7297 bits (208), Expect = 5.780e-17
Identity = 53/240 (22.08%), Postives = 106/240 (44.17%), Query Frame = 2
Query:  131 IEVLNLLTRK--TTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            +EV  LL  K  + + +   +N   P+H A    +  +  LL+       +       P+H+A K   L++ ++L+  G + ++    G +P HLA + GH D++  L+   + +          +H A + +++ +A+ L  +  ++         P+H+A  YG   + + L++    VNA+   G  P+H A   GH  ++  L + +   +         +  A +LG  S+V  L
Sbjct:  548 LEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 787          
BLAST of SMED30018624 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009608.1 (pep scaffold:Pmarinus_7.0:GL477242:23155:61557:-1 gene:ENSPMAG00000008655.1 transcript:ENSPMAT00000009608.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 124.02 bits (310), Expect = 6.433e-29
Identity = 110/450 (24.44%), Postives = 183/450 (40.67%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQY---------------------LIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDN-------NCNTNKTIYM----EHKE--VYH-----LFYSKKTKRIDRFKMHIG-------HHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNC 1306
            L +AC NGD+  V  L++    N  +       P+HFA       +VE L+ N  ++ A +D  + P+H AC +G+ ++V LL+  G D +  D    +P H A   G +D+   L+ +G+D   RN   +                      L+  A   N  ++   L   N N  A + +   P+H+A  Y    I  LL+Q   DV+A++  G  P+H AC  GH  + E L K     ++ + W   P+  A       +  LLL         NC++   I +    E KE  VY      L  + +   + R K H+G       H +  E+ L+ +    +          + + V  L          +++  + ++++ +   T  H+A    H +V+  LL  G + I   D  G   +H A    H+   R LL   C
Sbjct:  151 LFEACRNGDVGRVKRLVSPLNVNARDTTGRKSTPLHFAAGFGRKDVVEHLLQNGANVHARDDGGLIPLHNACSFGHAEVVNLLLRQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPGIRNTDGKTALDLAEPTAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHSKSAIDLAPTSELKERLVYEFRGHSLLQAAREADVTRVKKHLGLDIINFKHPQTHETALHCAVASPY---------PKRKQVSEL----------LLRKGANVNEKTKDLLTPLHVASDKAHNDVIDVLLKHG-AKINVTDSLGQTSLHRAAHAGHLTTCRLLLSHGC 580          

HSP 2 Score: 122.094 bits (305), Expect = 2.727e-28
Identity = 102/436 (23.39%), Postives = 188/436 (43.12%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFL-ISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL-DARLRNVRNLTNNCHSSAEDMIKCQSL-IDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            +V  LL RK  N+ E  ++L  P+H A   ++  ++++L+ +   I   +      +H A   G+L   +LL+S+GCD ++  L G +    A + G+  + + L  + S L  RN+  ++ +  A +   ++  K L  +   N      ++  P+H A  Y    +   L+Q   DV+A++  G  P+H AC  GH  + E L K     + A+ W   P+  A   G   I +LLL +  +  K                              +++  +PL +  + G  DI +L+  DA L +       C +  + +   +++   D + R  T  H+A    +L V +YLL+ G +D+  +D  G   +H A  + HVDI   L+  + C +  +K    P+H         +  L L+      + ++
Sbjct:  504 QVSELLLRKGANVNEKTKDLLTPLHVASDKAHNDVIDVLLKHGAKINVTDSLGQTSLHRAAHAGHLTTCRLLLSHGCDPNLPSLQGFT----AAQLGNESVQQIL--HESSLPVRNSDVEHQLLEAAKAGDLDTVKQLCTTQTVNCRDMEGRHSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKRGAVVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKK-----------------------------NRDGNTPLDL-VKEGDTDIQDLLRGDAALLDAAK--KGCLARVQKLCSGENVNCRDSQGRNSTPLHLAAGYNNLEVAEYLLEHG-ADVNAQDKGGLIPLHNAASYGHVDIAALLIKYSACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPSMKNQ 900          

HSP 3 Score: 112.079 bits (279), Expect = 3.232e-25
Identity = 73/280 (26.07%), Postives = 127/280 (45.36%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARN---------------NFSQYLIHNACRFNRIE------IAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN 868
            E+ L +A   GDL  V  L T +T N  ++      P+HFA   + V++VE L+ +  D+ A++   + P+H AC YG+ ++ +LL+  G  ++V DL   +P H A   G  +I K L+ +G+D   +N               +    L  +A   +  +      + K     N N      +   P+H+A  Y   ++   L++   DVNA++  G  P+H A   GH+ I   L K     ++ + W   P+  A + G   +  LLL +  + +
Sbjct:  617 EHQLLEAAKAGDLDTVKQLCTTQTVNCRDMEGRHSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKRGAVVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSGENVNCRDSQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYSACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPS 896          

HSP 4 Score: 99.3673 bits (246), Expect = 2.095e-21
Identity = 95/463 (20.52%), Postives = 195/463 (42.12%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-------------DLEARNNFSQYLIHNACRFNRIEIAK----FLISNNCNLEAPNYK----YDRPIHIACEYGF---EDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            K++ L +A  +G+  +++ LLT    N    +     P+H A   + V IV+LL+ +  D+ A++   + P+H AC YG+ ++ +LL+ +G  ++  DL   +P H A     +++   L+S G+             DL   +   + L++     + ++ A+      +  +  L+  N+K    ++  +H A    +   + +  LL+++  +VN +      P+H+A    H  +I+ L K     +  +      +  A   G  +  +LLL + C+ N    ++      L      + +    + +  + ++E  L  + + G LD V+ +   +  N R++    HS+                    H A     ++VV+YLL  G +D+  +D  G   +H AC + H ++   L+ R    ++ +     P+H   +     I  L L    
Sbjct:  300 KKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLVKHGASVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHSKSAIDLAPTSELKERLVYEFRGHSLLQAAREADVTRVKKHLGLDIINFKHPQTHETALHCAVASPYPKRKQVSELLLRKGANVNEKTKDLLTPLHVASDKAHNDVIDVLLKHGAKINVTDSLGQTSLHRAAHAGHLTTCRLLLSHGCDPN-LPSLQGFTAAQLGNESVQQILHESSLPV-RNSDVEHQLLEAAKAGDLDTVKQLCTTQTVNCRDMEGR-HSTP------------------LHFAAGYNRVSVVEYLLQHG-ADVHAKDKGGLVPLHNACSYGHYEVAELLVKRGAVVNVADLWKFTPLHEAAAKGKYEICKLLLKHGA 740          

HSP 5 Score: 78.1814 bits (191), Expect = 7.501e-15
Identity = 54/217 (24.88%), Postives = 97/217 (44.70%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISN---------GCDLSVNDL-------------DGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACIN 718
            EV  LL ++   +   +   + P+H A       I +LL+ +  D   +N     P+ L  K G+ DI  LL  +         GC   V  L               ++P HLA  + +L++ ++L+ +G+D+ A++      +HNA  +  ++IA  LI  +  + A +     P+H A + G   +C+LL+    D + +N  G  P+ LA  +
Sbjct:  697 EVAELLVKRGAVVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDL-VKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSGENVNCRDSQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYSACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPSMKNQEGQTPLDLATAD 912          

HSP 6 Score: 72.4034 bits (176), Expect = 4.450e-13
Identity = 76/421 (18.05%), Postives = 166/421 (39.43%), Query Frame = 2
Query:  236 IVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYG-FEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL----DARLRN-----VRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSG--DRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            + ELL+    ++  +    + P+H+A    + D++ +L+ +G  ++V D  G +  H A   GHL   + L+S+G D     N        A +     + + L  +  +L   N   +  +  A + G  + +  L   Q  +        + P+H A     + ++E+L +   D  + +   + P+  AC  G   + +LL+      N                      D +K          +PL+ +  +G  +I +L+L    D   +N       +L     +  +D+++  + + D  ++ C         L  VQ L      ++ C D  G     +H+A  ++++++  +LL+     +  +K    P+H   S  +  I  L +  +   + +D++     ++ ++K
Sbjct:  505 VSELLLRKGANVNEKTKDLLTPLHVASDKAHNDVIDVLLKHGAKINVTDSLGQTSLHRAAHAGHLTTCRLLLSHGCD----PNLPSLQGFTAAQLGNESVQQIL--HESSLPVRNSDVEHQLLEAAKAGDLDTVKQLCTTQTVNCRDMEGRHSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKRGAVVNVA--------------------DLWKF---------TPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGC---------LARVQKLCS--GENVNCRDSQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYSACVNATDKWAFTPLHEAAQK 879          
BLAST of SMED30018624 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 66.6254 bits (161), Expect = 1.178e-12
Identity = 47/195 (24.10%), Postives = 93/195 (47.69%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDI---EAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPS--HLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNL--EAPNYKYDR---PIHIACEYGFEDICSLLIQQ 655
            L QAC+  +  +V  LL  K + + + +++   P+H++++     I   L+S   ++   +  +D    P H+AC  GNL++V+ L        +N +     +  HLA      ++ +FLI NG+ +  ++ F+Q  +H A     +++ + L    C L   A N++  +   P+  A   G  D   LL+++
Sbjct:    6 LHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELL----CGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEK 196          

HSP 2 Score: 53.1434 bits (126), Expect = 4.913e-8
Identity = 53/208 (25.48%), Postives = 80/208 (38.46%), Query Frame = 2
Query:  299 PIHLACKYGNLDIVQ-LLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLIS-----NGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPI---------------HIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIE---HLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLD 850
            P+H AC       VQ LL S    L   D DG  P H +  F   +I  FL+S     N  D    + ++ +  H AC    +E+ K L             YDRP+               H+A    + ++   LI+    V  ++ +   P+H A   G L++IE    L K   +W     W   P+ +A   G      LL++
Sbjct:    5 PLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPF--HIACSVGNLEVVKSL-------------YDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWT--PLFHALAEGHGDAAVLLVE 195          
BLAST of SMED30018624 vs. Ensembl Yeast
Match: GDE1 (Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes [Source:SGD;Acc:S000006031])

HSP 1 Score: 56.225 bits (134), Expect = 2.250e-8
Identity = 48/162 (29.63%), Postives = 78/162 (48.15%), Query Frame = 2
Query:  266 DIEAEND-QKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNN-FSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYD-RPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIE 739
            D+ A  D + + P+HL C  G       ++    D +V  L  +S  HLA E+ +  ++  L+S+   D+  ++N   +  ++ ACR N  E A  L+ N  +LE     +    I +A   GF DI  LLI  N + + E+  G  P+  A + GHL I +
Sbjct:  464 DVSAFGDAESLTPLHL-CVLGAHPKTTEVLLQSLDPNVK-LKSSSLLHLATEWNNYPLLHVLLSSKRFDINYQDNELHETPLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIAD 623          
BLAST of SMED30018624 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672])

HSP 1 Score: 55.4546 bits (132), Expect = 3.924e-8
Identity = 47/213 (22.07%), Postives = 100/213 (46.95%), Query Frame = 2
Query:   86 EKENLLSQ---ACLNGDLIEVLNLLTRKTTNIEEINENL--YRPIHFAITNSNVAIVELLISNKCDIEAENDQ-KMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-----DLEARNNFSQY-LIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYG 688
            E++ LL++   AC  GDL  V  ++  K   +    ++      +H+A  N+ +++V+ L+S   D+ A        P+H A +YG + IV  L+ +G D ++ D  G +  HL+    ++ ++ +++ N       D++ R+   +  L+  A + + + +A+ L+    +++  + +   P+H     G   +   LIQ   D   +   G
Sbjct:   69 EEDPLLTRYHTACQRGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAE-LLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTG 280          

HSP 2 Score: 50.0618 bits (118), Expect = 1.711e-6
Identity = 47/211 (22.27%), Postives = 89/211 (42.18%), Query Frame = 2
Query:  272 EAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHL-----ACEFGHLDIIKFLISNGSDLEAR-NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACE--------YGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN 862
            EAE D  +   H AC+ G+L  V+ +I +G  L VN+ DG+S  H+     A     L ++ FL+S G+D+ AR        +H A R+  + I  FL+ +  +    + +    +H++          Y   ++ S   +   D++  +  G   +  A   G    +  L K       A+     P+ +    G   +++ L+ +  +
Sbjct:   67 EAEEDPLLTRYHTACQRGDLATVKEMI-HGKLLEVNN-DGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVS---KGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGAD 272          
BLAST of SMED30018624 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374])

HSP 1 Score: 54.6842 bits (130), Expect = 6.369e-8
Identity = 25/62 (40.32%), Postives = 42/62 (67.74%), Query Frame = 2
Query:  302 IHLACKYGNLDIVQLLISNGCDLSVNDLD--GNSPSHLACEFGHLDIIKFLISNGSDLEARN 481
            +H A   G+++IV+LLI NG D+++  ++  G++P   A   GHLD++K+L+ NG+D   RN
Sbjct:  368 LHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRN 429          

HSP 2 Score: 49.2914 bits (116), Expect = 3.102e-6
Identity = 27/92 (29.35%), Postives = 50/92 (54.35%), Query Frame = 2
Query:  302 IHLACKYGNLDIVQLLISNGCDLSVNDLD--GNSPSHLACEFGHLDIIKFLISNGSDLEARN--NFSQYLIHNACRFNRIEIAKFLISNNCN 565
            + +AC  G  D+V+ +I  G    +ND D  GN+  H A   GH++I++ LI NG+D+  ++   F    + +A     +++ K+L+ N  +
Sbjct:  334 LQIACDKGKYDVVKKMIEEGG-YDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGAD 424          
BLAST of SMED30018624 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465])

HSP 1 Score: 51.6026 bits (122), Expect = 7.036e-7
Identity = 54/212 (25.47%), Postives = 91/212 (42.92%), Query Frame = 2
Query:  299 PIHLACKYGNLDIV-QLLISNGC-DLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNR--IEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHL------------------ACINGHLRIIEHLFKVKCD---WDSANYWNMRPIEYACKLGVKSIVQLLLDNNC 859
            P+H A + G L+ V  LLI+N   D+   D D  +P  LA    H+D+++ L++ G               NA    +  ++ +K +IS+         ++D P+++AC++   D   LL++     NA+N+      HL                  A I G  ++I+ L +   D    D  N W   PI YA + G   ++  LL +N 
Sbjct:  427 PLHYAAELGKLEFVHSLLITNLLEDVDPIDSDSKTPLVLAITNNHIDVVRDLLTIGG-------------ANASPIEKPILDYSKNVISST------KVQFD-PLNVACKFNNHDAAKLLLEIRSKQNADNAKNKSSQHLCQPLFKKNSTGLCTLHIVAKIGGDPQLIQLLIRYGADPNEIDGFNKWT--PIFYAVRSGHSEVITELLKHNA 616          
BLAST of SMED30018624 vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11])

HSP 1 Score: 88.5817 bits (218), Expect = 9.061e-20
Identity = 51/185 (27.57%), Postives = 92/185 (49.73%), Query Frame = 2
Query:  299 PIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDS--ANYWNMRPIEYACKLG-VKSIVQLL 844
            P+H+ACK G++++ +LL+  G  L   +  G +P H+A   G  D +KFL+S G  ++ R+   +    +A    R E A  L+  + N ++       P+H+AC  G       L +     N  +  G   +H+A ++G  +++  L +   D ++  +N W   P+  A K G VK+ ++LL
Sbjct:   39 PLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKDENQKS-------PLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWT--PLMCAAKTGSVKTCMELL 214          

HSP 2 Score: 72.7886 bits (177), Expect = 2.622e-14
Identity = 47/196 (23.98%), Postives = 90/196 (45.92%), Query Frame = 2
Query:  281 NDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN 868
            +D     +H A +Y  ++ +  LI NG   S+ DL  ++P H+AC+ G +++ + L+  G+ L+ +N+  +  +H A      +  KFL+S    ++  ++ +  P   A   G  +   LL++++     EN     P+HLAC  G    +E L +     +  +      +  A   G + +V  LL    + N
Sbjct:    5 DDTGFSALHHATRYNRVEAMAELIRNG---SIEDL--STPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKD-----ENQ--KSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADIN 188          

HSP 3 Score: 60.077 bits (144), Expect = 4.524e-10
Identity = 57/242 (23.55%), Postives = 93/242 (38.43%), Query Frame = 2
Query:  500 IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECED 1225
            +H+A R+NR+E    LI N  ++E        P+H+AC+ G  ++  LL+++   ++ +N  G  P+H+A   G    ++ L       D  ++ +  P   A   G      LLL+ + N                                     +SPL+++C  G           R  +V  LT    S+ E          DE+ +   H+A  SG   VV  LL  G +DI   D
Sbjct:   12 LHHATRYNRVEAMAELIRNG-SIE----DLSTPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKDENQ------------------------------------KSPLHLACAAG-----------RALHVEALTRAGLSTNE---------RDEEGQSALHMAALSGFRKVVTQLLRYG-ADINARD 191          
BLAST of SMED30018624 vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8])

HSP 1 Score: 87.4261 bits (215), Expect = 1.007e-18
Identity = 58/226 (25.66%), Postives = 111/226 (49.12%), Query Frame = 2
Query:  311 ACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQ-QNCDV------------NAENSYGNR-------------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN---TNKTIYMEHKEVY 901
            A   G++D V+ L++ G D++  ++DG +  H AC   + D++K L+  G+ ++ R+N     +H A     +EIAK+LI N  ++ A N + + P+ +A E   ED+ +  I+ Q  DV            N   S+ N+              +H+A   G+L++I  L ++  + ++ +  N  P+  A   G K   +LL +N  +    NK + ++H++ +
Sbjct:   56 AVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSENGADFNARNKMVGIDHQQNW 281          
BLAST of SMED30018624 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 82.4185 bits (202), Expect = 2.616e-16
Identity = 55/199 (27.64%), Postives = 97/199 (48.74%), Query Frame = 2
Query:  242 ELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDG--NSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGH----LRIIEHLFKVKCDWDSANYWNMRPIEYACKLG 820
            +LL+ N   I+++ND+ + PIH A K+G +  V  L+     + +ND DG  N+P HLA   GH  ++K L+  G  ++ARN      +  A     +E+A  L+  +  ++  +     P+H+A   G  ++  LL+ +  ++   +S G   + +A  + H    + II+H+     DW  A       +   CKLG
Sbjct:  267 QLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAI-INDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAIIDHV-----DWKQA-------MRNRCKLG 452          

HSP 2 Score: 74.3294 bits (181), Expect = 9.752e-14
Identity = 71/323 (21.98%), Postives = 136/323 (42.11%), Query Frame = 2
Query:  269 IEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIK---------FLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYG-FEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLD-IGW 1204
            IE  +  K  P+ +A +  ++D V+ L+ +G D++  D++  +    A   G ++ ++         + I +   L      S  LI       RI  ++ L+ N   +++ N +   PIH A ++G    +C+LL +    +N E+   N P+HLA ++GH  +++ L       D+ N     P++ A   G+  +  +LLD +   + T                    D+ K          +PL+++ + GH+++V+L+L  +     N+T                + D   R C  IA  S H  V   ++D + W
Sbjct:  173 IECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLI----LVLRIS-SQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPT--------------------DKAKT---------TPLHLASREGHVEMVKLLLSRK----ANIT----------------LTDSAGRNCLDIAIESSHKEVAVAIIDHVDW 441          

HSP 3 Score: 72.7886 bits (177), Expect = 2.620e-13
Identity = 93/448 (20.76%), Postives = 175/448 (39.06%), Query Frame = 2
Query:  200 PIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLL-----ISNGCD-----LSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNA------CRFNRIEIAK----------------------------FLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACK---------------LGVKSIVQLLLDNN-CNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEI--------ESPLYVSCQRGHLDIV-ELILDARLRNVRNLT----NNCHSSAEDMIKCQSLIDDE---------KERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLD 1297
            P+H A ++  + + + L+    D+ + ++++M P+H AC  GNL + +LL     +  G       ++  D +  +  HLA E GH D+ K  +  G+++ A         H        C F  I+++K                            ++I     +E  +   D P+ IA E    D    L+Q   D+ A++      +  A   G +  +E           A   + +P+   C                L ++   QLLLDN  C  +K      +++  + Y+ K  R+      +     I         +PL+++   GH  +V EL+      + RN T     +C ++A  M++  +++ DE          +    H+A   GH+ +V+ LL    ++I   D +G   + IA   SH ++   ++D
Sbjct:    7 PLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLE-----------AALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKN----DEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDC-AAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRK-ANITLTDSAGRNCLDIAIESSHKEVAVAIID 437          

HSP 4 Score: 55.0694 bits (131), Expect = 1.152e-7
Identity = 85/399 (21.30%), Postives = 149/399 (37.34%), Query Frame = 2
Query:  290 KMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTK--RIDRFKMHIG-HHKEIESPLYVSCQRGHLDIVELIL---------DARLRN--VRNLTNNCHSSAEDMIKCQSLIDDEKERFCTH-IACSSGHLNVV-----QYLLDIGWSDIECEDMSGDR---LIHIACLFSHVDIVRFLLDRN-CCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +M P+H+AC +G + + + L+  G DL   D +  +P H AC  G+L + K L       E R  +S              ++K +          + +    +H+A E G  D+  L +++  +VNA     N+  H   +                           +   C   +  + +L   + C + K      +E+   F+        I RF+  I    K+ ++PL ++ ++ H+D V  +L         D   RN   R     C  + E  ++ Q  +    +  C + +   S  L +V     Q LLD G     C D   D     IH A  F  V  V  LL R+    +  +     P+H+     +A+++   L R  + D
Sbjct:    4 QMTPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAA---ELRGGWST-------------VSKMVTDQ-------DREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIV---------------------------VARECIFVMIKVSKLKYLSFCKSEKI----KREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNG----ACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAID 344          

HSP 5 Score: 52.373 bits (124), Expect = 6.998e-7
Identity = 33/143 (23.08%), Postives = 66/143 (46.15%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLI 550
            G +  V  LL R    I + +     P+H A  + + A+V+ L+     I+A N     P+  A   G +++  +L+     +   D    +P HLA   GH++++K L+S  +++   ++  +  +  A   +  E+A  +I
Sbjct:  294 GRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAII 436          
BLAST of SMED30018624 vs. Ensembl Nematostella
Match: EDO39113 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB41])

HSP 1 Score: 70.4774 bits (171), Expect = 9.189e-14
Identity = 46/188 (24.47%), Postives = 91/188 (48.40%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAEND---QKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGS-DLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQN 658
            QAC +G    V  LL    TN+  ++ + + P+H+A T ++V IV++L+    D++       Q++ PI  A K+  ++  ++L+  G D+++ +  G +  H A   GH  +++ L+ +G  D+   +  +   +H A       I K L+    N+ A +       H+A   G E++   L++  
Sbjct:    1 QACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188          

HSP 2 Score: 65.855 bits (159), Expect = 3.079e-12
Identity = 48/181 (26.52%), Postives = 80/181 (44.20%), Query Frame = 2
Query:  311 ACKYGNLDIV-QLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFK-VKCDWDSANYWNMRPIEYACKLGVKSIVQLLL 847
            AC+ G  D V +LL  N  +L+  D DG SP H A    H+DI++ L+  G+D++ R                               +P+ +   PI  A ++   + C +L++   DVN  N YG   +H A   GH +++E L +  + D +  +  N   +  A  +G   I++ LL
Sbjct:    2 ACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRG------------------------------SPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLL 152          

HSP 3 Score: 54.6842 bits (130), Expect = 1.843e-8
Identity = 38/177 (21.47%), Postives = 80/177 (45.20%), Query Frame = 2
Query:  236 IVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVND---LDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNN-CNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKV 754
            + +LL  N  ++   +     P+H A    ++DIVQ+L+  G D+ V     +    P   A +F  ++  + L+  G+D+   N + Q  +H+A R    ++ + L+ +   ++   +      +H+A   G   I   L+    ++ A+++ G    HLA   G   +++ L + 
Sbjct:   11 VRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLRT 187          
BLAST of SMED30018624 vs. Ensembl Nematostella
Match: EDO34704 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNN1])

HSP 1 Score: 62.3882 bits (150), Expect = 6.534e-12
Identity = 32/93 (34.41%), Postives = 54/93 (58.06%), Query Frame = 2
Query:  200 PIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGN--SPSHLACEFGHLDIIKFLISNGSDLE 472
            P+H A    NV  VELL+  + ++   +D++  P+H A + G + ++ LL+       VN LD N  +P HLA + GH+DI+ FL+  G+ ++
Sbjct:   11 PLHCAAHAQNVEAVELLLERRANVNVLDDREETPLHAAVRTGKIAVINLLLEGSNVRMVNWLDKNERTPLHLAAKHGHVDIVDFLLHTGASIQ 103          
BLAST of SMED30018624 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 149.828 bits (377), Expect = 3.369e-36
Identity = 115/481 (23.91%), Postives = 203/481 (42.20%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLD---------------------------------IIKFLISNGSDLEAR--NNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKK---TKRI--DRFKMHIGHHKEIESPLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCT--------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            + V  LL  +  N+    +N   P+H A    NV +V LL+     I+A+   ++ P+H A + G++ I+++L+ +G  +     +G SP H+A +  H+D                                 + K L+  G+   AR  N F+   +H AC+ N +     L+ ++ +LEA       P+H+A   G  +I   L+Q+    NA N     P+H+A   GH  + + L +     D+    +  P+  A ++G K +V+LLLD+  N +      H  + H+   +    T RI  D     +   K+  +PL+V+ + G +D+ EL+L+       A    +  L    H +  D++K   L+        T        HIA     + V   LL  G S   CE + G   +H+A      D+V  L+ +    ++ NK    P+H++    +  I    + +  S
Sbjct:  217 MSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTP--LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPL-HIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKL--LVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASP-NCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGAS 691          

HSP 2 Score: 148.288 bits (373), Expect = 1.063e-35
Identity = 106/475 (22.32%), Postives = 211/475 (44.42%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT-------IYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEKLEKALRIEKIFYCMENRIFPELEKM 1537
            +L +   N + +++  + P+H A    N+++ +LL++   ++       + P+H+A + GN+ +V+LL+  G  +     D  +P H A   GH+ II+ L+ +G+ ++A+       IH A + + ++  + L+  N  ++     +  P+H+A   G   +  +L+ +    NA    G  P+H+AC   H+R ++ L K     ++     + P+  A  +G  +IV+ LL    + N +       ++M     H EV   F  +   ++D         K+ ++PL+ + + GH ++V+L+LD +  N  + T   H+                     HIA   GH++ ++ LLD G   ++     G   +H+A  +  VD+   LL+R    +   K    P+H+    +   ++ L +S+  S        + N Y       K  +IE     ++N   P  E +
Sbjct:  189 VLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQ-FLLQNAAQVD------ARAKDDQTPLHCAARMGHKELVKLLLDHKA-NPDSATTAGHTP-------------------LHIAAREGHIHTIRILLDAGAQQVKMTK-KGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSA----HSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESL 631          

HSP 3 Score: 136.346 bits (342), Expect = 5.547e-32
Identity = 98/455 (21.54%), Postives = 187/455 (41.10%), Query Frame = 2
Query:  113 CLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKT----IYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIKC----QSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
               GD ++ +  L +    I++I  +   P+H A    +  + ++L+       A       P+H+ACK  ++  + LL+ +   L      G +P H+A   GHL+I+K L+  G+   A N   +  +H A R    E+A+FL+ N   ++A       P+H A   G +++  LL+    + ++  + G+ P+H+A   GH+  I  L                P+  A K G   + +LLL+   N N      +   H  V+H         +D  K+ +       S       PL+++ ++  +++  ++L          L+ +  L         DM+      Q+ ++   +   T  H+    GH+ +   L+  G S      M G   +H+AC + ++ +V+FLL +    +   +    P+H      +  I+ L L    
Sbjct:  310 AAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHH-------NNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRM-GYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGA 756          

HSP 4 Score: 119.013 bits (297), Expect = 1.101e-26
Identity = 106/485 (21.86%), Postives = 207/485 (42.68%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            +A  +G+L + L+ + +   NI   N+N    +H A    +V +V  L+ +  ++EA   +    +H+A   G   +V  L++ G +++     G SP ++A +  HL+++KFL+ NG++  L   + F+   +                           H A R +    A  L+ N+ N +  +     P+HIA  Y    +  LL+ +  +VN     G  P+H+A   G++ ++  L       D+     + P+  A + G   I+++LL++              +  +  + +   +D  +  + ++ EI+       +PL+V+   GH  + +++LD   + N R L            N   S + ++K  + ++   E   T  H+A   GHLN+V+ LL  G S     ++  +  +H+A    H ++ +FLL      D   K  Q P+H      +  ++ L L    + D
Sbjct:   16 RAARSGNLDKALDHI-KNGININIANQNGLNGLHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKT---KNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASP-NASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPD 495          

HSP 5 Score: 117.087 bits (292), Expect = 4.605e-26
Identity = 66/255 (25.88%), Postives = 121/255 (47.45%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLG---VKSIVQLLLDNNCNTNKTIYME 886
            ++V  LL  +  N     +N   P+H A+ ++N+ +V+LL+S      +       P+H+A K   +++  +L+ NG   +   L G +P HLA + G  D++  LIS  +++   N      +H   +   + IA  L+    ++ A +     P+H+AC YG   +   L+QQ   VNA+   G  P+H A   GH  I+  L K     +        P+  A +LG   V  +++L+ + + +T++  + E
Sbjct:  547 VDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVLKLVTEESVSTHRMSFPE 801          
BLAST of SMED30018624 vs. Ensembl Medaka
Match: ENSORLT00000031565.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 139.428 bits (350), Expect = 6.079e-33
Identity = 98/428 (22.90%), Postives = 184/428 (42.99%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + V ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V+++ G +  H+A   G  ++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  +  S+L++     +     G  P+H+    G L + + L + +   DSA    + P+  A     + +  LLLD   + + T    +  + H+   K    I    +  G       K+  +PL+++ Q GH D+  L++                 A+  +  +S +         H+A     + V + L+  G ++++ +   G   + +AC + +  +V FLL      +   K    P+H         I+ + L    
Sbjct:  388 VTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPL-HIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMS--------------KGAQINVPTKSGL------TALHLAAQEDRVAVAEILVRNG-ANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGA 793          

HSP 2 Score: 134.806 bits (338), Expect = 1.645e-31
Identity = 97/426 (22.77%), Postives = 179/426 (42.02%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDR-----FKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            LL +   N +  +++ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH   ++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + +  +  +   H+        + R       M     +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G L     LL+ G S        G   +H+   +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  226 LLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVS-NIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQLETASVLLEAGASH-SLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGAS 629          

HSP 3 Score: 127.872 bits (320), Expect = 2.148e-29
Identity = 114/512 (22.27%), Postives = 215/512 (41.99%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD--LSDEYKSINSYKKSEKLEKA 1474
            +A   G++ +VL  L +   +I   N+N    +H A    ++ +V+ L+     +++   +    +H++   G  ++V+ L+  G D++    +G +P ++A +  HLD++++L+ NG +      + F+   I                           H A R +  + A  L+ N+ N +  +     P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  ++  L       D+     + P+  A + G ++ V++LL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A      ++VR LL      D   +  Q P+H+        I+ L L      D   ++ Y  ++   +  +LE A
Sbjct:   53 RAARAGNIDKVLEYL-KGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETA 554          

HSP 4 Score: 116.701 bits (291), Expect = 5.866e-26
Identity = 100/480 (20.83%), Postives = 187/480 (38.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE-----------------------------------ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDN----NCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAE-DMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            + V  LL  +   ++    N   P+H A    N  +V LL+     I+A+    + P+H A + G+   V++L+  G  L     +G SP H+A +  H++ +K L+ + + ++                                   A N F+   +H AC+ NR+++ + L+    +++A       PIH+A   G  +I  LL+Q     +  N  G   +H+A   G   ++  L +     D+    +  P+  A +LG   IVQLLL +    +  T       H           +  ++    H    K+  +PL+V  + G LD+ +L+L  +       +N     H +A  D  K   L+ D+     T         HIA     +++   LL  G ++       G   +H+A    H D+   L+ +    ++  K     +H+       ++  + +    + D
Sbjct:  254 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP--LHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYG-AETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLD 730          

HSP 5 Score: 110.923 bits (276), Expect = 3.819e-24
Identity = 110/499 (22.04%), Postives = 192/499 (38.48%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSD--------------------------------IECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R    ++   +     +H +       +V+ L+    DI A++     P+++A +  +LD+V+ L+ NG + S    DG +P  +A + GH  ++  L+ N    + +       +H A R +  + A  L+ N+ N +  +     P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  ++  L       D+     + P+  A + G ++ V++LL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S                                 ++         +HIA      +IV+ LL      D        P+H+           + L    S  L+
Sbjct:   82 NALHLAAKEGHMDLVQELLDRGAP-VDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLA 567          

HSP 6 Score: 108.227 bits (269), Expect = 2.912e-23
Identity = 62/238 (26.05%), Postives = 105/238 (44.12%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+               P+H+A K   +DI  +L+  G + +     G +P HLA + GH D+   L+S G+ +          +H A + +R+ +A+ L+ N  NL+        P+ +AC YG   + S L+Q    VNA+   G  P+H A   G+  II  L +     ++        +  A +LG  S+V  L
Sbjct:  584 LDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 821          

HSP 7 Score: 51.6026 bits (122), Expect = 4.877e-6
Identity = 26/108 (24.07%), Postives = 51/108 (47.22%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFL 448
            D + V  +L R   N++   +  Y P+  A    N  +V  L+ N   + A+      P+H A + GN  I+ +L+ +G   +   ++GN+   +A   G++ ++  L
Sbjct:  714 DRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 821          
BLAST of SMED30018624 vs. Ensembl Medaka
Match: ENSORLT00000039458.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 139.043 bits (349), Expect = 9.130e-33
Identity = 98/428 (22.90%), Postives = 184/428 (42.99%), Query Frame = 2
Query:  137 VLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHH-----KEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            V  LL  K  N      N + P+H A   + V ++ELL+     I+A  +  + PIH+A   G+L+IV LL+ NG    V+++ G +  H+A   G  ++++ L+ NG+ ++AR    Q  +H A R  + EI + L+ +  + +A       P+HI+   G  +  S+L++     +     G  P+H+    G L + + L + +   DSA    + P+  A     + +  LLLD   + + T    +  + H+   K    I    +  G       K+  +PL+++ Q GH D+  L++                 A+  +  +S +         H+A     + V + L+  G ++++ +   G   + +AC + +  +V FLL      +   K    P+H         I+ + L    
Sbjct:  373 VTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPL-HIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMS--------------KGAQINVPTKSGL------TALHLAAQEDRVAVAEILVRNG-ANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGA 778          

HSP 2 Score: 134.42 bits (337), Expect = 2.599e-31
Identity = 97/426 (22.77%), Postives = 179/426 (42.02%), Query Frame = 2
Query:  146 LLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDR-----FKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            LL +   N +  +++ + P+H A    NV +  LL++    ++      + P+H+A K GN ++V+LL+  G  +     DG +P H A   GH   ++ L+  G+ L AR       +H A + + +E  K L+ +   ++     Y   +H+A   G   +  LL+ +  + NA    G  P+H+AC    ++++E L K      +     + PI  A  +G  +IV LLL N  + + +  +  +   H+        + R       M     +E ++PL+++ + G  +IV+L+L   + +    T N ++                     HI+   G L     LL+ G S        G   +H+   +  +D+ + LL R    D   K    P+H+        +  L L +  S
Sbjct:  211 LLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVS-NIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQ-HMAHPDAATTNGYTP-------------------LHISAREGQLETASVLLEAGASH-SLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGAS 614          

HSP 3 Score: 127.487 bits (319), Expect = 2.960e-29
Identity = 114/512 (22.27%), Postives = 215/512 (41.99%), Query Frame = 2
Query:  107 QACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD--LEARNNFSQYLI---------------------------HNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD--LSDEYKSINSYKKSEKLEKA 1474
            +A   G++ +VL  L +   +I   N+N    +H A    ++ +V+ L+     +++   +    +H++   G  ++V+ L+  G D++    +G +P ++A +  HLD++++L+ NG +      + F+   I                           H A R +  + A  L+ N+ N +  +     P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  ++  L       D+     + P+  A + G ++ V++LL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S  +  ++ G+  +H+A      ++VR LL      D   +  Q P+H+        I+ L L      D   ++ Y  ++   +  +LE A
Sbjct:   38 RAARAGNIDKVLEYL-KGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASP-DVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETA 539          

HSP 4 Score: 115.931 bits (289), Expect = 9.913e-26
Identity = 100/480 (20.83%), Postives = 187/480 (38.96%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLE-----------------------------------ARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDN----NCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARL---RNVRNLTNNCHSSAE-DMIKCQSLIDDEKERFCT---------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFD 1414
            + V  LL  +   ++    N   P+H A    N  +V LL+     I+A+    + P+H A + G+   V++L+  G  L     +G SP H+A +  H++ +K L+ + + ++                                   A N F+   +H AC+ NR+++ + L+    +++A       PIH+A   G  +I  LL+Q     +  N  G   +H+A   G   ++  L +     D+    +  P+  A +LG   IVQLLL +    +  T       H           +  ++    H    K+  +PL+V  + G LD+ +L+L  +       +N     H +A  D  K   L+ D+     T         HIA     +++   LL  G ++       G   +H+A    H D+   L+ +    ++  K     +H+       ++  + +    + D
Sbjct:  239 VNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTP--LHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYG-AETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLD 715          

HSP 5 Score: 110.538 bits (275), Expect = 4.711e-24
Identity = 110/499 (22.04%), Postives = 192/499 (38.48%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNC-------NTNKTIYM----EHKE-VYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLR-NVRNLTN----------NCHSSAEDMIKCQSLIDDEKERFCT--HIACSSGHLNVVQYLLDIGWSD--------------------------------IECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLS 1420
            N L  A   G +  V  LL R    ++   +     +H +       +V+ L+    DI A++     P+++A +  +LD+V+ L+ NG + S    DG +P  +A + GH  ++  L+ N    + +       +H A R +  + A  L+ N+ N +  +     P+HIA  YG  ++ +LL+ +   V+     G  P+H+A   G+  ++  L       D+     + P+  A + G ++ V++LL+          N    ++M    +H E V HL   K    +D   +      +  + L+V+   GH  + +L+LD R   N R L            N     E ++K  + I    E   T  H+A   GHLN+V  LL  G S                                 ++         +HIA      +IV+ LL      D        P+H+           + L    S  L+
Sbjct:   67 NALHLAAKEGHMDLVQELLDRGAP-VDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAP--VDDVTL------DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLA 552          

HSP 6 Score: 107.842 bits (268), Expect = 3.472e-23
Identity = 62/238 (26.05%), Postives = 105/238 (44.12%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLL 844
            ++V  LL ++    +   +N   P+H A    N  +  LL+               P+H+A K   +DI  +L+  G + +     G +P HLA + GH D+   L+S G+ +          +H A + +R+ +A+ L+ N  NL+        P+ +AC YG   + S L+Q    VNA+   G  P+H A   G+  II  L +     ++        +  A +LG  S+V  L
Sbjct:  569 LDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 806          

HSP 7 Score: 51.6026 bits (122), Expect = 5.484e-6
Identity = 26/108 (24.07%), Postives = 51/108 (47.22%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFL 448
            D + V  +L R   N++   +  Y P+  A    N  +V  L+ N   + A+      P+H A + GN  I+ +L+ +G   +   ++GN+   +A   G++ ++  L
Sbjct:  699 DRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 806          
BLAST of SMED30018624 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 137.502 bits (345), Expect = 2.312e-32
Identity = 108/448 (24.11%), Postives = 194/448 (43.30%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLIS----NKCDIEAEND-------QKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLD-------NNCNTNKTIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            +EV+  L     N     E+ + P+  A+   +  +V LLIS     K  + A  D           P+H+A  Y NL++ QLL++ G +      D  +P H A   GH  II+ L+ NG+ ++A+       IH A + + ++  K L+  N  ++     +  P+H+A   G   +  +L+ +    N+    G  P+H+AC   HLR+++ L K     ++     + P+  A  +G  +IV++LL        +N      ++M     H EV   F  +    +D         K+ ++PL+ + + GH ++V+L+L+ +  N  + T   HS                     HIA   GH+  V+ LLD+     +     G   +H+A  +  VD+   LL+R    +   K    P+H+    +   ++ L +S+  S
Sbjct:  119 LEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGAN------DELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAE-FLLQNAAPVD------AKAKDDQTPLHCAARMGHKELVKLLLEHKA-NPNSTTTAGHSP-------------------LHIAAREGHVQTVRLLLDMEAQQTKMTK-KGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGS 532          

HSP 2 Score: 135.576 bits (340), Expect = 8.921e-32
Identity = 101/440 (22.95%), Postives = 184/440 (41.82%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHI-------GHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCS 1408
            A  NG    ++ +L      I+   +N   PIH A    ++  V+ L+    +I+      + P+H+A   G+  + ++L+  G   +   L+G +P H+AC+  HL ++  L+ + + +EA        +H A     + I K L+    +  A N K + P+H+A   G  ++   L+Q    V+A+      P+H A   GH  +++ L + K + +S       P+  A + G    V+LLLD      K   M  K    L  + K  ++D  ++ +          K   +PL+V+    +LD+V L++            + HS+A +                 HIA     + V   LL  G S    E + G   +H+A      DIV  L+ +    ++ NK    P+H++    +  I  + + +  S
Sbjct:  217 AARNGHF-RIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTK---MTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSK--------GGSPHSAARNGYT------------ALHIAAKQNQVEVANSLLQHGAS-ANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGAS 631          

HSP 3 Score: 132.494 bits (332), Expect = 8.293e-31
Identity = 76/258 (29.46%), Postives = 127/258 (49.22%), Query Frame = 2
Query:  134 EVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMR----PIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDL--EARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEH 889
            E++ LL     N        + P+H A    +V  V LL+    D+EA+  +  +    P+H+A KYG +D+ +LL+  G + +    +G +P H+A    +LD++  L+S G      ARN ++   +H A + N++E+A  L+ +  +  A + +   P+H+A + G  DI SLLI +  +VN  N  G  P+HL    GH+ I + L K      +A      P+  AC  G   +V+ LL    N N    +E+
Sbjct:  422 ELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLL----DMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTA--LHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSKTRVEY 673          

HSP 4 Score: 131.724 bits (330), Expect = 1.338e-30
Identity = 88/378 (23.28%), Postives = 167/378 (44.18%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-----PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNC 1306
            N + P+H A   +++ +++LL+ +   IEA  +  + P+H+A   G+L+IV++L+  G   S +++   +P H+A   GH ++ +FL+ N + ++A+    Q  +H A R    E+ K L+ +  N  +       P+HIA   G      LL+            G  P+H+A   G + + E L +   + ++A    + P+  A       +V LL+    + +      +  + H+   +    +    +  G     ES     PL+++ Q G  DIV L++ ++  NV NL N    +                    H+    GH+ +   L+  G S      M G   +H+AC + ++ +V+FLL +  
Sbjct:  308 NGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTAL-HIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLI-SKQANV-NLGNKSGLTP------------------LHLVAQEGHVGIADILVKQGASVYAATRM-GYTPLHVACHYGNIKMVKFLLQQQA 663          

HSP 5 Score: 125.176 bits (313), Expect = 1.688e-28
Identity = 111/468 (23.72%), Postives = 202/468 (43.16%), Query Frame = 2
Query:   95 NLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSD----LEARNNFSQYL-------IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-------SPLYVSCQRGHLDIVELILD----ARLRNVRNLTNNCHSS-------AEDMIKCQSLIDDEKERFCTHIACSS--GHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNC 1405
            N L  A   G +  VL LL      +E   +     +H A       +V  L++   ++ A++ +   P+++A +  +L++V+ L+ NG + S+   DG +P  +A + GH +++  LIS G+     L A  +F  +L       +H A  +  + +A+ L++   N E        P+H A   G   I  +L+     + A+   G  PIH+A    H+  ++ L +   + D     ++ P+  A   G   + ++LLD     N         + H+   K   R+    + + H   IE       +PL+V+   GHL+IV+++L         NV+  T    +S       AE +++  + +D + +   T + C++  GH  +V+ LL+   ++      +G   +HIA    HV  VR LLD       M K+   P+H+        +  L L R  
Sbjct:   42 NALHLASKEGHVKMVLELLHNGIV-LETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDEL------TPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPL-HIACKKNHLRV--MDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLE-HKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGA 498          

HSP 6 Score: 117.087 bits (292), Expect = 4.182e-26
Identity = 101/436 (23.17%), Postives = 171/436 (39.22%), Query Frame = 2
Query:  251 ISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNC-------------------------------DVNAENSYGNR-------PIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-----------------SPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            I N  DI   N   +  +HLA K G++ +V  L+ NG  L      GN+  H+A   G   ++  L++ G+++ A++      ++ A + N +E+ KFL+ N  N   P      P+ +A + G E++ +LLI                                   ++N      NR       P+H A  NGH RIIE L        +     + PI  A +      V+ LL  N   +  I ++H    H+              H GHH+  +                 +PL+++C++ HL +++L+L              HS++ + +    L          H+A   GHLN+V+ LL  G S     ++  +  +H+A    H ++  FLL      D   K  Q P+H      +  ++ L L
Sbjct:   27 IKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDD-ITLDHLTPLHV------------AAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLK-------------HSASIEAVTESGLTP-------LHVASFMGHLNIVKILLQKGASP-SASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLL 428          
BLAST of SMED30018624 vs. Ensembl Medaka
Match: ankrd44 (ankyrin repeat domain 44 [Source:NCBI gene;Acc:101173198])

HSP 1 Score: 134.42 bits (337), Expect = 1.333e-31
Identity = 107/466 (22.96%), Postives = 201/466 (43.13%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAI-VELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYME-HKEVYHLFYSKKTKRIDRFKMHIGHHKEIE-----SPLYVSCQRGHLDIVELILDARLRNVRNLTNNC--------------HSSA-EDMIKCQSLID--DEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNC---CYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            AC NG    V++ L     N+ + N   + P+HFA  +++ A+ +E L++N  DI  ++     P+H+   +G     Q LI NG +++  D DGN+P H+A  +GH  +I  LI++G+D   R     + +H A      +  + L+S+   ++ P+      +H A   G  D   LL++   + N  +  G  P+H A  + H   +E L     D ++ + W    + YA              ++ +  + + +E   E       K+  +   F +  G    ++     SP++ +   GH   +EL+LD    + R    +C              H+ A + +++    +D  D+  R    +A   GH + V  LL  G S +  + + G   +H+A +  H   VR LLD        D+ + + + P+ +     +   + L L R  + + +D+
Sbjct:  196 ACFNGQ-DAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAA------------SDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTH--DGRQEEADCGNAQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADK 646          

HSP 2 Score: 105.916 bits (263), Expect = 9.648e-23
Identity = 96/455 (21.10%), Postives = 192/455 (42.20%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRI-IEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEY-KSINSYKKSEKLEKALRI 1483
            ++++ + +L R + ++   ++N   P+H A  N+ +   E++I     +   +      +H A   G+ ++V LL++ G +++  D       H A   GHLD++  L+S+G+++  ++      +H A    +I + K L+S +  ++ PN   +  +H+AC  G + + S LI    +V+  N+ G  P+H A  + H  + +E L     D +  +     P+      G  +  Q L+ N    N                     +D         K+  +PL+V+ + GH    EL++           N   +S  D  +           F  H+A  + H +  + LL  G+  I+  D  G   +H A    +VD V+ LL     ++  +K  + P+H   +S +   L   +      + +D++ +S   Y  +  L++  R+
Sbjct:   35 NVLDAVQVLIRHSADVNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINS--------------------VD---------KDGNTPLHVAARYGH----ELLI-----------NTLITSGADCAR-----RGVHGMFPLHLAALNAHSDCCRKLLSSGFQ-IDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRV 439          

HSP 3 Score: 103.99 bits (258), Expect = 3.310e-22
Identity = 108/508 (21.26%), Postives = 202/508 (39.76%), Query Frame = 2
Query:  101 LSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDII----------------------------------KFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTI--------YMEHKEVYHLFYS--------KKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDI-----------VELILD----ARLRNVRNLTNNCHSSAEDMIKCQSLIDD-----EKERFCT------HIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQK-PIHMIPSSSYASILPLFL 1393
            L  A LNG   E++NLL  K  NI   ++   R +H+A    ++ +V +L+S+  ++  ++ +   P+H A   G + +V+ L+S   ++   +  GN+  HLAC  G   ++                                  +FL++NG+D+  ++   +  +H      R   ++ LI N   + + +   + P+H+A  YG E + + LI    D      +G  P+HLA +N H      L       D+ +      +  A   G    VQLLL +    N+T         Y      YH   +          T +  R  +H     +++    V+ +     +           +E +L     A L++ +  +   +++A     C  L+ D     ++E  C       H+A   GH   +Q LL  G  +++  D +G   + +A L  H D V  LL +       ++ Y + P+H+   + + + + L L
Sbjct:   94 LHHAALNGH-TEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGNAQSPLHLAAYHGHAQALQVLLR-GGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLL 599          

HSP 4 Score: 92.8189 bits (229), Expect = 1.097e-18
Identity = 100/449 (22.27%), Postives = 171/449 (38.08%), Query Frame = 2
Query:  200 PIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNAC-----RFNRIEIA------------------KFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGN--RPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEI--------ESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQS--------------LIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIEC----EDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFL 1393
            P+H A  N++      L+S+   I+  +      +H A   GN+D VQLL+ +G + +  D  G +P H A    H   ++ L+  G+D+ A + + +  +H A      R  R+ +                   +FL+ +       + +   P+H A  +G      LL+  +     E   GN   P+HLA  +GH + ++ L +   + D  +     P+  A   G    V  LL    +   T  +  +   HL          R  +      E+         +PL ++   GH+D V L+L+ R  NV         +    + C                L  D + R   H+A + GH + +  LL I  S+       +D SG   +H AC +     V  LL++  C  I    +  P+H    + + S   L L
Sbjct:  325 PLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGNAQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLE-REANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQRGCRCIDGNPF-TPLHCAVVNGHESCAALLL 771          

HSP 5 Score: 68.5514 bits (166), Expect = 3.246e-11
Identity = 49/229 (21.40%), Postives = 103/229 (44.98%), Query Frame = 2
Query:  128 LIEVLNLLTRKTTNIEEINENL-YRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLI-SNGCDLSV-NDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCD---VNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRP 796
            L E+L +   +++++  + +   Y P+H+A        VE+L+  +      +     P+H A   G+     LL+ + G D++   D  G +P H A   G  + I+ L+S+ + ++  ++  +  +  A    R+   + L+     L   +   + P+H+AC  G ED   +++Q+  D   +NA N+    P+H+A   G  + ++ L        + +   + P
Sbjct:  697 LSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQR-GCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGNTPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGASTQTVDEDGLTP 924          

HSP 6 Score: 64.6994 bits (156), Expect = 5.024e-10
Identity = 68/340 (20.00%), Postives = 128/340 (37.65%), Query Frame = 2
Query:  122 GDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLS-----VNDLDGNSPSHLACEFGHLDIIKFLISN---------------------------------GSDLEA-RNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGH----LRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHL 1012
            G  ++ ++LL  +  N+   ++     +H A+  +    V+ L+     +   + Q   PIHLA   G+   +  L++  C  S     + D  G +P H AC +G    ++ L+                                   GSD+   R+   +  +H A    + E  + L+S++  ++  +     P+  A   G       L+     ++  +  GN P+H+AC NG     L I++ L       ++ N     P+  A + G+K  VQ LL    +T     ++   +        ++++     H G H     PL +   RG L
Sbjct:  625 GGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQRGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGNTPLHVACSNGTEDGVLMILQRLSDATL-INATNAAMQTPLHMAAQRGLKKAVQELLSRGASTQT---VDEDGLTPALACAPSRQVADCLAHSGSHDAFLLPLQL---RGPL 957          

HSP 7 Score: 63.929 bits (154), Expect = 8.370e-10
Identity = 66/295 (22.37%), Postives = 119/295 (40.34%), Query Frame = 2
Query:  200 PIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLI----SNGSDLEARNNFSQYL-IHNACRFNRIEIAKFLISN-NCN-LEAPNYKYDRPIHIACEYGFEDICSLLIQQ-NCDVNA-ENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKT--------KRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELIL 1033
            P+  A+   +V  V LL+  + ++   + + +  +HLA      + VQ L+     L   D  G +P HLA   GH   +  L+    S  S +    + S Y  +H AC + +    + L+    C  ++   +    P+H A   G E   +LL+     D+    ++ G  P+H A   G    I+ L       D A+     P+  A   G    ++ LLD     +  I  +     H+  S  T        +R+    +    +  +++PL+++ QRG    V+ +L
Sbjct:  618 PLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQRGCRCIDGNPFT---PLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLS-LIDRDGNTPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELL 908          
BLAST of SMED30018624 vs. Planmine SMEST
Match: SMESG000052676.1 (SMESG000052676.1)

HSP 1 Score: 1253.04 bits (3241), Expect = 0.000e+0
Identity = 611/612 (99.84%), Postives = 611/612 (99.84%), Query Frame = 2
Query:   83 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEKLEKALRIEKIFYCMENRIFPELEKMIFQYYSQYRSEIIFRLSCYFTGSIQMTKPSNSLKKMCKLICLIDNDNRIVVGKIEIIPEFYNRKLNSQARKYQRTEKICRKMCEICFTNEINYRFPCGHVTCSYCSWNADFCYYCWVPNNGGKFIQQ 1918
            MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEKLEKALRIEKIFYCMENRIFPELEKMIFQYYSQYRSEIIFRLSCYFTGSIQMTKPSNSLKKMCKLICLIDNDNRIVVGKIEIIPEFYNRKLNSQARKYQRTEKICRKMCEICFTNEINYRFPCGHVTCSYCSWNADFCYYCWVPNNGGKFI Q
Sbjct:    1 MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEKLEKALRIEKIFYCMENRIFPELEKMIFQYYSQYRSEIIFRLSCYFTGSIQMTKPSNSLKKMCKLICLIDNDNRIVVGKIEIIPEFYNRKLNSQARKYQRTEKICRKMCEICFTNEINYRFPCGHVTCSYCSWNADFCYYCWVPNNGGKFIHQ 612          
BLAST of SMED30018624 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 285.034 bits (728), Expect = 1.242e-81
Identity = 166/464 (35.78%), Postives = 257/464 (55.39%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSIN 1441
            AC +G   +++ LL     +IE       RPIHFA     + IV+LLI +KCDIEA + +   PIH AC+ G+ DIV+LLI   CD+   D     P H+ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA       PIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GH  I++ L   KCD +SA+  N RPI +AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     +++K      C     D   +   H+AC+ GH  VV+ L+D    DIE  D S  R I++AC   H+DIV+ L+D+ C  +  +   Q+PIH+    ++  I+ L + +NC  +  ++ K ++
Sbjct:  138 ACKHG-FPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID-QHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLS 596          

HSP 2 Score: 273.092 bits (697), Expect = 1.707e-77
Identity = 160/454 (35.24%), Postives = 247/454 (54.41%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQ-KPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            K+  +  AC  G L E++ LL  +  +IE   E+   PIH A  + +  IV+LLI   CDIE+ ++   RPI++ACK G+ DIV+LLI   CD+   D     P H ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA      RPIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GHL I++ L    CD +SA+  N RPI  AC  G   +V+LL+D +C+         + +Y    + K   +D  K+ I    +IES       P++V+C++ HL+IV+L+          +  NC   A +  K  S           H+AC +GHL +V+ L++   +   C   + +R IH AC   H++IV+ L+D++C  + +  R++ +PIH      +  I+ L +   C  +  D+
Sbjct:  263 KQRPIHVACEKGKL-EIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIY---VACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLL----------IGQNCDIEAFNKRKGLS--------HPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDK 694          

HSP 3 Score: 270.011 bits (689), Expect = 2.095e-76
Identity = 160/464 (34.48%), Postives = 247/464 (53.23%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDA-------RLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            I+++ LL     +IE ++ +L +PIH A  +    IV+LLI + CDIEA      RPIH AC+ G LDIV+LLI + CD+   D     P H ACE GH DI+K LI    D+E+ +N +Q  IH AC    ++I K LI   C++E+ +    RPIH+ACE G  +I  LLI Q+CD+ A       PIH+AC +GH  I++ L    CD +SA+  N RPI  ACK G   IV+LL+D  C+         + ++   ++ +   +D  K+ I    +IES       P++V+C++G L+IV+L++D        +  N R +   C     D++K      C     D   +   ++AC  GHL +V+ L++    DIE  D    R IH+AC   H ++V+ L+D++C  +  +   Q+PI++     +  I+ L + + C  + +D YK    +   EK
Sbjct:  111 IKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIH---FACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN-QHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEK 570          

HSP 4 Score: 265.388 bits (677), Expect = 9.841e-75
Identity = 182/591 (30.80%), Postives = 289/591 (48.90%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLR-------NVRNLTNNCHSSAEDMIKCQSLIDD--------EKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK---------LEKALRIEKIFYCMENRIFPELEKMIFQYYSQYRSEIIFRLSCYFTGSIQMTKPSNSLKKMCKLICLIDNDNRIVVGKIEIIPEFYNRKLNSQARKYQRTEKICRKMCE 1789
            AC  G L  V+  L  +  N+E       +PIH A    ++ IVELLI +KCDIEA +   ++PIHLACK+G  DIV+LLI + CD+         P H ACE G LDI+K LI +  D+EA +  ++  IH AC     +I K LI   C++E+ +    RPIH+ACE G  DI  LLI Q CD+ + ++Y  RPIH+AC  G L I++ L    CD ++    N  PI  AC+ G   IV+LL+D +C+         + +Y    + K    D  K+ I    +IES       P++ +C++GHLDIV+L++D +           R +   C     +++K   LID         E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H++IV+ L++++C  +  +   Q+PIH+  +  +  ++ L + ++C  + +D       Y   ++         +++   IE      +  I    EK   +       +++   +C      +    S+ +   CK             G +EI+    N+K N +A +YQ +E+     CE
Sbjct:   72 ACKQGHL-NVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIY---VACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVK--LLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIV-----KLLIGQNCDIEAFNKRKGLSHPIHLACK------------NGHLEIVKLLINKKCNIEACRYQNSERAIHFACE 638          

HSP 5 Score: 246.514 bits (628), Expect = 3.134e-68
Identity = 160/478 (33.47%), Postives = 249/478 (52.09%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFS--QYLIHNACRFNRIEIAKFLISNNCNLEAPNYK-YDRPIHIACEYGFEDICSLLIQQNCDVNA-ENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-------KTIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEY--KSINSYKKSEKLEKALRIEKI 1492
            AC  G L E++ LL  +  +IE  +    RPIH A    +  +V+LLI   CDIE+ ++   RPI++ACK G+LDIV+LLI   CD+   D     P H+ACE  HL+I+K LI    D+EA N      + IH AC+   +EI K LI+  CN+EA  Y+  +R IH ACE G  +I  LLI Q+CD+ A    + NRPIH AC  GHL I++ L   KCD ++ +  N RPI +AC+     I ++L++  CN         + I++     H E+  L +  K       + +IG    +  P++ +C  GHL+I++L++  +     N  N C  S                    + ACS GHL +V+ L+D    D E  D    + IH AC   H++I + L+D  C  + ++K  ++PIH   +  +  I+ L + +NC  +  +++  + I+  K+  +++    +EKI
Sbjct:  468 ACKKGHL-EIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNI--EVQENIG----LLRPIHFACNNGHLEIMKLLIAQKCD--INAVNKCFRSP------------------VYFACSKGHLEIVKLLIDQNC-DTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKI 917          

HSP 6 Score: 238.039 bits (606), Expect = 3.097e-65
Identity = 147/431 (34.11%), Postives = 227/431 (52.67%), Query Frame = 2
Query:  197 RPIHFAITNSNVAIVELLISNKCDIEAEN--DQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMI------KCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            +PI  A  N ++ IV+LL+S+KCDIEA +  D   R IH ACK G+L++V+ L+   C++         P H A   GH+ I++ LI +  D+EA +      IH AC+    +I K LI +NC++EA      RPIH ACE G  DI  LLI   CD+ A +     PIH AC  GH  I++ L   KCD +SA+  N RPI  AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     D++      KC     D   +   H AC  GHL++V+ L+D    DIE  D    R IH+AC    ++IV+ L+D++C  +   +  Q+PIH+     +  I+ L + ++C  + +D
Sbjct:   32 KPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLID-QKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESAD 458          

HSP 7 Score: 223.402 bits (568), Expect = 2.184e-60
Identity = 134/399 (33.58%), Postives = 214/399 (53.63%), Query Frame = 2
Query:  293 MRPIHLACKYGNLDIVQLLISNGCDL-SVNDLDGNSPS-HLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            ++PI LAC+ G+L+IV+LL+S+ CD+ +V+ +D    S H AC+ GHL+++KFL+    ++EA        IH A R   I+I + LI + C++EA +    +PIH+AC++GF DI  LLI  NCD+ A      RPIH AC  G L I++ L   KCD ++ +  N  PI +AC+ G   IV+LL+D  C+         + ++    + +   +D  K+ I    +IES       P++V+C++G L+IV+L++D                  D+  C+     E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H DIV+ L+D+ C  +  +   Q+PIH      +  I+ L + + C  + +D YK    +   EK
Sbjct:   31 IKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIH---VACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHC---------------DIEACK-----ESNQCPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEK 405          

HSP 8 Score: 182.956 bits (463), Expect = 4.221e-47
Identity = 103/259 (39.77%), Postives = 147/259 (56.76%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLY-RPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYL-IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN 862
            E  +  AC  G L E++ LL  +  +IE I      RPIHFA    ++ IV+LLI +KCDIEA +    RPIH AC+  +L+I ++LI   C++   D++   P HLAC +GH +I+  L     ++E + N      IH AC    +EI K LI+  C++ A N  +  P++ AC  G  +I  LLI QNCD  A +   ++PIH AC  GHL I + L   KCD ++ +  N RPI +AC  G   IV+LL+D NC+
Sbjct:  630 ERAIHFACEQGHL-EIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCD 887          

HSP 9 Score: 158.303 bits (399), Expect = 2.844e-39
Identity = 89/212 (41.98%), Postives = 123/212 (58.02%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEA-ENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK 757
            D +E+  +L  K  NIE  + N  RPIH A +  +  IV LL   KC+IE  EN   +RPIH AC  G+L+I++LLI+  CD++  +    SP + AC  GHL+I+K LI    D EA +  +   IH ACR   + I K LI + C++EA +    RPIH AC  G  DI  LLI QNCD+ A N +  RPI +A     ++I++ L K+K
Sbjct:  707 DHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKIK 918          
BLAST of SMED30018624 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 284.648 bits (727), Expect = 1.493e-81
Identity = 166/464 (35.78%), Postives = 257/464 (55.39%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSIN 1441
            AC +G   +++ LL     +IE       RPIHFA     + IV+LLI +KCDIEA + +   PIH AC+ G+ DIV+LLI   CD+   D     P H+ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA       PIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GH  I++ L   KCD +SA+  N RPI +AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     +++K      C     D   +   H+AC+ GH  VV+ L+D    DIE  D S  R I++AC   H+DIV+ L+D+ C  +  +   Q+PIH+    ++  I+ L + +NC  +  ++ K ++
Sbjct:  127 ACKHG-FPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID-QHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLS 585          

HSP 2 Score: 272.707 bits (696), Expect = 2.153e-77
Identity = 160/454 (35.24%), Postives = 247/454 (54.41%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQ-KPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            K+  +  AC  G L E++ LL  +  +IE   E+   PIH A  + +  IV+LLI   CDIE+ ++   RPI++ACK G+ DIV+LLI   CD+   D     P H ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA      RPIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GHL I++ L    CD +SA+  N RPI  AC  G   +V+LL+D +C+         + +Y    + K   +D  K+ I    +IES       P++V+C++ HL+IV+L+          +  NC   A +  K  S           H+AC +GHL +V+ L++   +   C   + +R IH AC   H++IV+ L+D++C  + +  R++ +PIH      +  I+ L +   C  +  D+
Sbjct:  252 KQRPIHVACEKGKL-EIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIY---VACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLL----------IGQNCDIEAFNKRKGLS--------HPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDK 683          

HSP 3 Score: 269.626 bits (688), Expect = 2.472e-76
Identity = 160/464 (34.48%), Postives = 247/464 (53.23%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDA-------RLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            I+++ LL     +IE ++ +L +PIH A  +    IV+LLI + CDIEA      RPIH AC+ G LDIV+LLI + CD+   D     P H ACE GH DI+K LI    D+E+ +N +Q  IH AC    ++I K LI   C++E+ +    RPIH+ACE G  +I  LLI Q+CD+ A       PIH+AC +GH  I++ L    CD +SA+  N RPI  ACK G   IV+LL+D  C+         + ++   ++ +   +D  K+ I    +IES       P++V+C++G L+IV+L++D        +  N R +   C     D++K      C     D   +   ++AC  GHL +V+ L++    DIE  D    R IH+AC   H ++V+ L+D++C  +  +   Q+PI++     +  I+ L + + C  + +D YK    +   EK
Sbjct:  100 IKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIH---FACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN-QHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEK 559          

HSP 4 Score: 265.003 bits (676), Expect = 1.260e-74
Identity = 182/591 (30.80%), Postives = 289/591 (48.90%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLR-------NVRNLTNNCHSSAEDMIKCQSLIDD--------EKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK---------LEKALRIEKIFYCMENRIFPELEKMIFQYYSQYRSEIIFRLSCYFTGSIQMTKPSNSLKKMCKLICLIDNDNRIVVGKIEIIPEFYNRKLNSQARKYQRTEKICRKMCE 1789
            AC  G L  V+  L  +  N+E       +PIH A    ++ IVELLI +KCDIEA +   ++PIHLACK+G  DIV+LLI + CD+         P H ACE G LDI+K LI +  D+EA +  ++  IH AC     +I K LI   C++E+ +    RPIH+ACE G  DI  LLI Q CD+ + ++Y  RPIH+AC  G L I++ L    CD ++    N  PI  AC+ G   IV+LL+D +C+         + +Y    + K    D  K+ I    +IES       P++ +C++GHLDIV+L++D +           R +   C     +++K   LID         E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H++IV+ L++++C  +  +   Q+PIH+  +  +  ++ L + ++C  + +D       Y   ++         +++   IE      +  I    EK   +       +++   +C      +    S+ +   CK             G +EI+    N+K N +A +YQ +E+     CE
Sbjct:   61 ACKQGHL-NVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIY---VACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVK--LLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIV-----KLLIGQNCDIEAFNKRKGLSHPIHLACK------------NGHLEIVKLLINKKCNIEACRYQNSERAIHFACE 627          

HSP 5 Score: 246.128 bits (627), Expect = 3.810e-68
Identity = 160/478 (33.47%), Postives = 249/478 (52.09%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFS--QYLIHNACRFNRIEIAKFLISNNCNLEAPNYK-YDRPIHIACEYGFEDICSLLIQQNCDVNA-ENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-------KTIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEY--KSINSYKKSEKLEKALRIEKI 1492
            AC  G L E++ LL  +  +IE  +    RPIH A    +  +V+LLI   CDIE+ ++   RPI++ACK G+LDIV+LLI   CD+   D     P H+ACE  HL+I+K LI    D+EA N      + IH AC+   +EI K LI+  CN+EA  Y+  +R IH ACE G  +I  LLI Q+CD+ A    + NRPIH AC  GHL I++ L   KCD ++ +  N RPI +AC+     I ++L++  CN         + I++     H E+  L +  K       + +IG    +  P++ +C  GHL+I++L++  +     N  N C  S                    + ACS GHL +V+ L+D    D E  D    + IH AC   H++I + L+D  C  + ++K  ++PIH   +  +  I+ L + +NC  +  +++  + I+  K+  +++    +EKI
Sbjct:  457 ACKKGHL-EIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNI--EVQENIG----LLRPIHFACNNGHLEIMKLLIAQKCD--INAVNKCFRSP------------------VYFACSKGHLEIVKLLIDQN-CDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKI 906          

HSP 6 Score: 237.269 bits (604), Expect = 3.940e-65
Identity = 147/431 (34.11%), Postives = 227/431 (52.67%), Query Frame = 2
Query:  197 RPIHFAITNSNVAIVELLISNKCDIEAEN--DQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMI------KCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            +PI  A  N ++ IV+LL+S+KCDIEA +  D   R IH ACK G+L++V+ L+   C++         P H A   GH+ I++ LI +  D+EA +      IH AC+    +I K LI +NC++EA      RPIH ACE G  DI  LLI   CD+ A +     PIH AC  GH  I++ L   KCD +SA+  N RPI  AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     D++      KC     D   +   H AC  GHL++V+ L+D    DIE  D    R IH+AC    ++IV+ L+D++C  +   +  Q+PIH+     +  I+ L + ++C  + +D
Sbjct:   21 KPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLID-QKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESAD 447          

HSP 7 Score: 223.016 bits (567), Expect = 2.636e-60
Identity = 134/399 (33.58%), Postives = 214/399 (53.63%), Query Frame = 2
Query:  293 MRPIHLACKYGNLDIVQLLISNGCDL-SVNDLDGNSPS-HLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSINSYKKSEK 1462
            ++PI LAC+ G+L+IV+LL+S+ CD+ +V+ +D    S H AC+ GHL+++KFL+    ++EA        IH A R   I+I + LI + C++EA +    +PIH+AC++GF DI  LLI  NCD+ A      RPIH AC  G L I++ L   KCD ++ +  N  PI +AC+ G   IV+LL+D  C+         + ++    + +   +D  K+ I    +IES       P++V+C++G L+IV+L++D                  D+  C+     E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H DIV+ L+D+ C  +  +   Q+PIH      +  I+ L + + C  + +D YK    +   EK
Sbjct:   20 IKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIH---VACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHC---------------DIEACK-----ESNQCPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEK 394          

HSP 8 Score: 182.57 bits (462), Expect = 5.002e-47
Identity = 103/259 (39.77%), Postives = 147/259 (56.76%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLY-RPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYL-IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN 862
            E  +  AC  G L E++ LL  +  +IE I      RPIHFA    ++ IV+LLI +KCDIEA +    RPIH AC+  +L+I ++LI   C++   D++   P HLAC +GH +I+  L     ++E + N      IH AC    +EI K LI+  C++ A N  +  P++ AC  G  +I  LLI QNCD  A +   ++PIH AC  GHL I + L   KCD ++ +  N RPI +AC  G   IV+LL+D NC+
Sbjct:  619 ERAIHFACEQGHL-EIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCD 876          

HSP 9 Score: 157.918 bits (398), Expect = 3.516e-39
Identity = 89/212 (41.98%), Postives = 123/212 (58.02%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEA-ENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK 757
            D +E+  +L  K  NIE  + N  RPIH A +  +  IV LL   KC+IE  EN   +RPIH AC  G+L+I++LLI+  CD++  +    SP + AC  GHL+I+K LI    D EA +  +   IH ACR   + I K LI + C++EA +    RPIH AC  G  DI  LLI QNCD+ A N +  RPI +A     ++I++ L K+K
Sbjct:  696 DHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKIK 907          
BLAST of SMED30018624 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 284.648 bits (727), Expect = 4.768e-81
Identity = 166/464 (35.78%), Postives = 257/464 (55.39%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSIN 1441
            AC +G   +++ LL     +IE       RPIHFA     + IV+LLI +KCDIEA + +   PIH AC+ G+ DIV+LLI   CD+   D     P H+ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA       PIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GH  I++ L   KCD +SA+  N RPI +AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     +++K      C     D   +   H+AC+ GH  VV+ L+D    DIE  D S  R I++AC   H+DIV+ L+D+ C  +  +   Q+PIH+    ++  I+ L + +NC  +  ++ K ++
Sbjct:  211 ACKHG-FPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID-QHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLS 669          

HSP 2 Score: 273.092 bits (697), Expect = 5.676e-77
Identity = 160/454 (35.24%), Postives = 247/454 (54.41%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQ-KPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            K+  +  AC  G L E++ LL  +  +IE   E+   PIH A  + +  IV+LLI   CDIE+ ++   RPI++ACK G+ DIV+LLI   CD+   D     P H ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA      RPIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GHL I++ L    CD +SA+  N RPI  AC  G   +V+LL+D +C+         + +Y    + K   +D  K+ I    +IES       P++V+C++ HL+IV+L+          +  NC   A +  K  S           H+AC +GHL +V+ L++   +   C   + +R IH AC   H++IV+ L+D++C  + +  R++ +PIH      +  I+ L +   C  +  D+
Sbjct:  336 KQRPIHVACEKGKL-EIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIY---VACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLL----------IGQNCDIEAFNKRKGLS--------HPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDK 767          

HSP 3 Score: 269.626 bits (688), Expect = 7.637e-76
Identity = 158/455 (34.73%), Postives = 244/455 (53.63%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDA-------RLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKS 1435
            I+++ LL     +IE ++ +L +PIH A  +    IV+LLI + CDIEA      RPIH AC+ G LDIV+LLI + CD+   D     P H ACE GH DI+K LI    D+E+ +N +Q  IH AC    ++I K LI   C++E+ +    RPIH+ACE G  +I  LLI Q+CD+ A       PIH+AC +GH  I++ L    CD +SA+  N RPI  ACK G   IV+LL+D  C+         + ++   ++ +   +D  K+ I    +IES       P++V+C++G L+IV+L++D        +  N R +   C     D++K      C     D   +   ++AC  GHL +V+ L++    DIE  D    R IH+AC   H ++V+ L+D++C  +  +   Q+PI++     +  I+ L + + C  + +D YK 
Sbjct:  184 IKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIH---FACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN-QHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQ 634          

HSP 4 Score: 265.003 bits (676), Expect = 3.806e-74
Identity = 160/460 (34.78%), Postives = 246/460 (53.48%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLR-------NVRNLTNNCHSSAEDMIKCQSLIDD--------EKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            AC  G L  V+  L  +  N+E       +PIH A    ++ IVELLI +KCDIEA +   ++PIHLACK+G  DIV+LLI + CD+         P H ACE G LDI+K LI +  D+EA +  ++  IH AC     +I K LI   C++E+ +    RPIH+ACE G  DI  LLI Q CD+ + ++Y  RPIH+AC  G L I++ L    CD ++    N  PI  AC+ G   IV+LL+D +C+         + +Y    + K    D  K+ I    +IES       P++ +C++GHLDIV+L++D +           R +   C     +++K   LID         E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H++IV+ L++++C  +  +   Q+PIH+  +  +  ++ L + ++C  + +D
Sbjct:  145 ACKQGHL-NVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIY---VACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVK--LLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESAD 597          

HSP 5 Score: 246.128 bits (627), Expect = 9.539e-68
Identity = 160/478 (33.47%), Postives = 249/478 (52.09%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFS--QYLIHNACRFNRIEIAKFLISNNCNLEAPNYK-YDRPIHIACEYGFEDICSLLIQQNCDVNA-ENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-------KTIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEY--KSINSYKKSEKLEKALRIEKI 1492
            AC  G L E++ LL  +  +IE  +    RPIH A    +  +V+LLI   CDIE+ ++   RPI++ACK G+LDIV+LLI   CD+   D     P H+ACE  HL+I+K LI    D+EA N      + IH AC+   +EI K LI+  CN+EA  Y+  +R IH ACE G  +I  LLI Q+CD+ A    + NRPIH AC  GHL I++ L   KCD ++ +  N RPI +AC+     I ++L++  CN         + I++     H E+  L +  K       + +IG    +  P++ +C  GHL+I++L++  +     N  N C  S                    + ACS GHL +V+ L+D    D E  D    + IH AC   H++I + L+D  C  + ++K  ++PIH   +  +  I+ L + +NC  +  +++  + I+  K+  +++    +EKI
Sbjct:  541 ACKKGHL-EIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNI--EVQENIG----LLRPIHFACNNGHLEIMKLLIAQKCD--INAVNKCFRSP------------------VYFACSKGHLEIVKLLIDQNC-DTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKI 990          

HSP 6 Score: 244.973 bits (624), Expect = 2.335e-67
Identity = 168/495 (33.94%), Postives = 255/495 (51.52%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEIN-ENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQK-MRPIHLACKYGNLDIVQLLISNGCDL-SVNDLDGNSPS-HLACEFGHLDIIKFL---------------------------------ISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMI------KCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            AC NG L E++ LL  K  NIE    +N  R IHFA    ++ IV+LLI   CDIEA + +K ++PI LAC+ G+L+IV+LL+S+ CD+ +V+ +D    S H AC+ GHL+++KFL                                 I +  D+EA +      IH AC+    +I K LI +NC++EA      RPIH ACE G  DI  LLI   CD+ A +     PIH AC  GH  I++ L   KCD +SA+  N RPI  AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     D++      KC     D   +   H AC  GHL++V+ L+D    DIE  D    R IH+AC    ++IV+ L+D++C  +   +  Q+PIH+     +  I+ L + ++C  + +D
Sbjct:   42 ACKNGHL-EIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLID-QKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESAD 531          

HSP 7 Score: 237.654 bits (605), Expect = 7.894e-65
Identity = 154/460 (33.48%), Postives = 234/460 (50.87%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQ-KMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGN-SPSHLACEFGHLDIIKFLISNGSDLEA--RNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAP------------------------NYKYD---------RPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKS 1435
             L  PIH A  N ++ IV+LLI+ KC+IEA   Q   R IH AC+ G+L+IV+LLI   CD+   D   N  P  LAC  GHL+I+K L+S+  D+EA  + + ++  IH AC+   + + KFL+  NCN+EA                         ++K D         +PIH+AC++GF DI  LLI  NCD+ A      RPIH AC  G L I++ L   KCD ++ +  N  PI +AC+ G   IV+LL+D  C+         + ++    + +   +D  K+ I    +IES       P++V+C++G L+IV+L++D                  D+  C+     E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H DIV+ L+D+ C  +  +   Q+PIH      +  I+ L + + C  + +D YK 
Sbjct:   34 GLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIH---VACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHC---------------DIEACK-----ESNQCPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQ 469          

HSP 8 Score: 182.956 bits (463), Expect = 6.087e-47
Identity = 103/259 (39.77%), Postives = 147/259 (56.76%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLY-RPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYL-IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN 862
            E  +  AC  G L E++ LL  +  +IE I      RPIHFA    ++ IV+LLI +KCDIEA +    RPIH AC+  +L+I ++LI   C++   D++   P HLAC +GH +I+  L     ++E + N      IH AC    +EI K LI+  C++ A N  +  P++ AC  G  +I  LLI QNCD  A +   ++PIH AC  GHL I + L   KCD ++ +  N RPI +AC  G   IV+LL+D NC+
Sbjct:  703 ERAIHFACEQGHL-EIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCD 960          

HSP 9 Score: 158.303 bits (399), Expect = 3.885e-39
Identity = 89/212 (41.98%), Postives = 123/212 (58.02%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEA-ENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK 757
            D +E+  +L  K  NIE  + N  RPIH A +  +  IV LL   KC+IE  EN   +RPIH AC  G+L+I++LLI+  CD++  +    SP + AC  GHL+I+K LI    D EA +  +   IH ACR   + I K LI + C++EA +    RPIH AC  G  DI  LLI QNCD+ A N +  RPI +A     ++I++ L K+K
Sbjct:  780 DHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKIK 991          
BLAST of SMED30018624 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 284.648 bits (727), Expect = 5.327e-81
Identity = 166/464 (35.78%), Postives = 257/464 (55.39%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKSIN 1441
            AC +G   +++ LL     +IE       RPIHFA     + IV+LLI +KCDIEA + +   PIH AC+ G+ DIV+LLI   CD+   D     P H+ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA       PIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GH  I++ L   KCD +SA+  N RPI +AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     +++K      C     D   +   H+AC+ GH  VV+ L+D    DIE  D S  R I++AC   H+DIV+ L+D+ C  +  +   Q+PIH+    ++  I+ L + +NC  +  ++ K ++
Sbjct:  212 ACKHG-FPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID-QHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLS 670          

HSP 2 Score: 272.707 bits (696), Expect = 6.390e-77
Identity = 160/454 (35.24%), Postives = 247/454 (54.41%), Query Frame = 2
Query:   89 KENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQ-KPIHMIPSSSYASILPLFLSRNCSFDLSDE 1426
            K+  +  AC  G L E++ LL  +  +IE   E+   PIH A  + +  IV+LLI   CDIE+ ++   RPI++ACK G+ DIV+LLI   CD+   D     P H ACE GHLDI+K LI    D+E+ +N+ Q  IH AC   ++EI K LI  +C++EA      RPIH+ACE+G  DI  LLI Q+CD+ + ++   RPI++AC  GHL I++ L    CD +SA+  N RPI  AC  G   +V+LL+D +C+         + +Y    + K   +D  K+ I    +IES       P++V+C++ HL+IV+L+          +  NC   A +  K  S           H+AC +GHL +V+ L++   +   C   + +R IH AC   H++IV+ L+D++C  + +  R++ +PIH      +  I+ L +   C  +  D+
Sbjct:  337 KQRPIHVACEKGKL-EIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIY---VACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLL----------IGQNCDIEAFNKRKGLS--------HPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDK 768          

HSP 3 Score: 269.626 bits (688), Expect = 8.429e-76
Identity = 158/455 (34.73%), Postives = 244/455 (53.63%), Query Frame = 2
Query:  131 IEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDA-------RLRNVRNLTNNCHSSAEDMIK------CQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKS 1435
            I+++ LL     +IE ++ +L +PIH A  +    IV+LLI + CDIEA      RPIH AC+ G LDIV+LLI + CD+   D     P H ACE GH DI+K LI    D+E+ +N +Q  IH AC    ++I K LI   C++E+ +    RPIH+ACE G  +I  LLI Q+CD+ A       PIH+AC +GH  I++ L    CD +SA+  N RPI  ACK G   IV+LL+D  C+         + ++   ++ +   +D  K+ I    +IES       P++V+C++G L+IV+L++D        +  N R +   C     D++K      C     D   +   ++AC  GHL +V+ L++    DIE  D    R IH+AC   H ++V+ L+D++C  +  +   Q+PI++     +  I+ L + + C  + +D YK 
Sbjct:  185 IKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIH---FACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN-QHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQ 635          

HSP 4 Score: 264.618 bits (675), Expect = 4.238e-74
Identity = 160/460 (34.78%), Postives = 246/460 (53.48%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLR-------NVRNLTNNCHSSAEDMIKCQSLIDD--------EKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            AC  G L  V+  L  +  N+E       +PIH A    ++ IVELLI +KCDIEA +   ++PIHLACK+G  DIV+LLI + CD+         P H ACE G LDI+K LI +  D+EA +  ++  IH AC     +I K LI   C++E+ +    RPIH+ACE G  DI  LLI Q CD+ + ++Y  RPIH+AC  G L I++ L    CD ++    N  PI  AC+ G   IV+LL+D +C+         + +Y    + K    D  K+ I    +IES       P++ +C++GHLDIV+L++D +           R +   C     +++K   LID         E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H++IV+ L++++C  +  +   Q+PIH+  +  +  ++ L + ++C  + +D
Sbjct:  146 ACKQGHL-NVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIY---VACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVK--LLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESAD 598          

HSP 5 Score: 246.128 bits (627), Expect = 1.048e-67
Identity = 160/478 (33.47%), Postives = 249/478 (52.09%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFS--QYLIHNACRFNRIEIAKFLISNNCNLEAPNYK-YDRPIHIACEYGFEDICSLLIQQNCDVNA-ENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTN-------KTIYME----HKEVYHLFYSKKTKRIDRFKMHIGHHKEIESPLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEY--KSINSYKKSEKLEKALRIEKI 1492
            AC  G L E++ LL  +  +IE  +    RPIH A    +  +V+LLI   CDIE+ ++   RPI++ACK G+LDIV+LLI   CD+   D     P H+ACE  HL+I+K LI    D+EA N      + IH AC+   +EI K LI+  CN+EA  Y+  +R IH ACE G  +I  LLI Q+CD+ A    + NRPIH AC  GHL I++ L   KCD ++ +  N RPI +AC+     I ++L++  CN         + I++     H E+  L +  K       + +IG    +  P++ +C  GHL+I++L++  +     N  N C  S                    + ACS GHL +V+ L+D    D E  D    + IH AC   H++I + L+D  C  + ++K  ++PIH   +  +  I+ L + +NC  +  +++  + I+  K+  +++    +EKI
Sbjct:  542 ACKKGHL-EIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNI--EVQENIG----LLRPIHFACNNGHLEIMKLLIAQKCD--INAVNKCFRSP------------------VYFACSKGHLEIVKLLIDQNC-DTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKI 991          

HSP 6 Score: 244.973 bits (624), Expect = 2.565e-67
Identity = 168/495 (33.94%), Postives = 255/495 (51.52%), Query Frame = 2
Query:  110 ACLNGDLIEVLNLLTRKTTNIEEIN-ENLYRPIHFAITNSNVAIVELLISNKCDIEAENDQK-MRPIHLACKYGNLDIVQLLISNGCDL-SVNDLDGNSPS-HLACEFGHLDIIKFL---------------------------------ISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILD-------ARLRNVRNLTNNCHSSAEDMI------KCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSD 1423
            AC NG L E++ LL  K  NIE    +N  R IHFA    ++ IV+LLI   CDIEA + +K ++PI LAC+ G+L+IV+LL+S+ CD+ +V+ +D    S H AC+ GHL+++KFL                                 I +  D+EA +      IH AC+    +I K LI +NC++EA      RPIH ACE G  DI  LLI   CD+ A +     PIH AC  GH  I++ L   KCD +SA+  N RPI  AC+ G   IV+LL+D  C+       + + ++    + +  +++  K+ I  H +IE+       P++V+C+ GH DIV+L++D       A   N R +   C     D++      KC     D   +   H AC  GHL++V+ L+D    DIE  D    R IH+AC    ++IV+ L+D++C  +   +  Q+PIH+     +  I+ L + ++C  + +D
Sbjct:   43 ACKNGHL-EIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIH---VACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLID-QKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESAD 532          

HSP 7 Score: 237.654 bits (605), Expect = 8.498e-65
Identity = 154/460 (33.48%), Postives = 234/460 (50.87%), Query Frame = 2
Query:  188 NLYRPIHFAITNSNVAIVELLISNKCDIEAENDQ-KMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGN-SPSHLACEFGHLDIIKFLISNGSDLEA--RNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAP------------------------NYKYD---------RPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIES-------PLYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKERFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIVRFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYKS 1435
             L  PIH A  N ++ IV+LLI+ KC+IEA   Q   R IH AC+ G+L+IV+LLI   CD+   D   N  P  LAC  GHL+I+K L+S+  D+EA  + + ++  IH AC+   + + KFL+  NCN+EA                         ++K D         +PIH+AC++GF DI  LLI  NCD+ A      RPIH AC  G L I++ L   KCD ++ +  N  PI +AC+ G   IV+LL+D  C+         + ++    + +   +D  K+ I    +IES       P++V+C++G L+IV+L++D                  D+  C+     E  +   H+AC  GH ++V+ L+D    DIE  D S  R I++AC   H DIV+ L+D+ C  +  +   Q+PIH      +  I+ L + + C  + +D YK 
Sbjct:   35 GLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIH---VACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHC---------------DIEACK-----ESNQCPIHVACEHGHFDIVKLLID-QHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQ 470          

HSP 8 Score: 182.956 bits (463), Expect = 6.326e-47
Identity = 103/259 (39.77%), Postives = 147/259 (56.76%), Query Frame = 2
Query:   92 ENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLY-RPIHFAITNSNVAIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYL-IHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSIVQLLLDNNCN 862
            E  +  AC  G L E++ LL  +  +IE I      RPIHFA    ++ IV+LLI +KCDIEA +    RPIH AC+  +L+I ++LI   C++   D++   P HLAC +GH +I+  L     ++E + N      IH AC    +EI K LI+  C++ A N  +  P++ AC  G  +I  LLI QNCD  A +   ++PIH AC  GHL I + L   KCD ++ +  N RPI +AC  G   IV+LL+D NC+
Sbjct:  704 ERAIHFACEQGHL-EIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCD 961          

HSP 9 Score: 158.303 bits (399), Expect = 4.031e-39
Identity = 89/212 (41.98%), Postives = 123/212 (58.02%), Query Frame = 2
Query:  125 DLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNVAIVELLISNKCDIEA-ENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVNDLDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIEIAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENSYGNRPIHLACINGHLRIIEHLFKVK 757
            D +E+  +L  K  NIE  + N  RPIH A +  +  IV LL   KC+IE  EN   +RPIH AC  G+L+I++LLI+  CD++  +    SP + AC  GHL+I+K LI    D EA +  +   IH ACR   + I K LI + C++EA +    RPIH AC  G  DI  LLI QNCD+ A N +  RPI +A     ++I++ L K+K
Sbjct:  781 DHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLIAQKCDINAVNKCFRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLLEKIK 992          
The following BLAST results are available for this feature:
BLAST of SMED30018624 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK23.114e-3724.53ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK23.535e-3724.53ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK23.738e-3724.53ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK23.738e-3724.53ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
ANK23.748e-3724.53ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
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BLAST of SMED30018624 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-446.151e-3524.14AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-446.151e-3524.14AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-446.428e-3524.14AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-446.428e-3524.14AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-447.941e-3524.14AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of SMED30018624 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank22.670e-3724.83gene:FBgn0261788 transcript:FBtr0303119[more]
Ank27.181e-3724.83gene:FBgn0261788 transcript:FBtr0334061[more]
Ank4.181e-3525.35gene:FBgn0011747 transcript:FBtr0089172[more]
Ank4.181e-3525.35gene:FBgn0011747 transcript:FBtr0300497[more]
Ank4.181e-3525.35gene:FBgn0011747 transcript:FBtr0089171[more]
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BLAST of SMED30018624 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1b7.476e-3823.70ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b7.688e-3823.86ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b9.204e-3823.86ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank3a3.270e-3723.02ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ank3a3.270e-3723.02ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
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BLAST of SMED30018624 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK12.281e-3724.57ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK12.658e-3724.57ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK12.937e-3724.57ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK13.428e-3724.57ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK13.531e-3724.57ankyrin 1 [Source:NCBI gene;Acc:613080][more]
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BLAST of SMED30018624 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank21.420e-3624.53ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank21.446e-3624.53ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank35.260e-3522.09ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank36.044e-3522.09ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank36.187e-3522.09ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
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BLAST of SMED30018624 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|G5E8K5|ANK3_MOUSE4.953e-3422.09Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV... [more]
sp|Q01484|ANK2_HUMAN1.368e-3224.76Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=... [more]
sp|Q8C8R3|ANK2_MOUSE2.941e-3224.76Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV... [more]
sp|P16157|ANK1_HUMAN2.701e-3122.02Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=... [more]
sp|O70511|ANK3_RAT3.617e-3122.09Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE... [more]
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BLAST of SMED30018624 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A182GGY87.506e-5227.58ANK_REP_REGION domain-containing protein OS=Aedes ... [more]
A0A355KTI74.719e-5128.97ANK_REP_REGION domain-containing protein OS=Candid... [more]
Q16FI91.942e-4824.95AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
W4YMA41.975e-4825.00ANK_REP_REGION domain-containing protein OS=Strong... [more]
A0A1X7T5X62.254e-4729.46Uncharacterized protein OS=Amphimedon queenslandic... [more]
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BLAST of SMED30018624 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank3a1.630e-3622.09ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ank3a1.982e-3622.09ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ank1a6.014e-3623.01ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b1.196e-3423.61ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank3b2.709e-3322.90ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
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BLAST of SMED30018624 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000093.16.698e-3723.72pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000007449.17.584e-3724.23pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
ENSPMAT00000007446.11.270e-3622.85pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000000095.11.756e-3623.57pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
ENSPMAT00000009608.16.433e-2924.44pep scaffold:Pmarinus_7.0:GL477242:23155:61557:-1 ... [more]
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BLAST of SMED30018624 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
NAS61.178e-1224.10Evolutionarily conserved 19S regulatory particle a... [more]
GDE12.250e-829.63Glycerophosphocholine (GroPCho) phosphodiesterase;... [more]
AKR13.924e-822.07Palmitoyl transferase involved in protein palmitoy... [more]
HOS46.369e-840.32Subunit of the Set3 complex; complex is a meiotic-... [more]
PHO817.036e-725.47Cyclin-dependent kinase (CDK) inhibitor; regulates... [more]
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BLAST of SMED30018624 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO387259.061e-2027.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO436901.007e-1825.66Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387022.616e-1627.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO391139.189e-1424.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347046.534e-1234.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30018624 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1b3.369e-3623.91ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
ENSORLT00000031565.16.079e-3322.90ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ENSORLT00000039458.19.130e-3322.90ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ank1a2.312e-3224.11ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ankrd441.333e-3122.96ankyrin repeat domain 44 [Source:NCBI gene;Acc:101... [more]
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BLAST of SMED30018624 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052676.10.000e+099.84SMESG000052676.1[more]
SMESG000017135.11.242e-8135.78SMESG000017135.1[more]
SMESG000017135.11.493e-8135.78SMESG000017135.1[more]
SMESG000017135.14.768e-8135.78SMESG000017135.1[more]
SMESG000017135.15.327e-8135.78SMESG000017135.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018624 ID=SMED30018624|Name=SMED30018624|organism=Schmidtea mediterranea sexual|type=transcript|length=2981bp
GTTATTTAGATAAAAACATTAATATTAAGGTAATTTGTTTTAATATTATA
ATATATAAATTTATTACAATTCACAAATTTGAATGGAAAAAGAAAATTTA
TTATCTCAAGCCTGTTTAAATGGTGATCTAATTGAGGTATTGAATTTGTT
AACTAGAAAAACTACTAACATTGAAGAAATTAATGAAAATTTATACAGAC
CAATACATTTTGCCATAACAAATAGTAATGTGGCTATTGTCGAATTATTA
ATATCTAACAAATGCGATATTGAAGCTGAAAATGATCAAAAAATGAGACC
AATCCACCTGGCCTGTAAATATGGAAATCTCGATATAGTTCAATTATTAA
TTTCAAATGGATGTGATCTGAGCGTCAATGATTTGGATGGAAATAGTCCA
TCGCATTTAGCTTGTGAGTTTGGACACCTTGATATTATCAAATTTTTAAT
TTCAAATGGATCCGATTTAGAAGCAAGGAATAATTTCTCTCAGTATTTGA
TTCACAATGCATGCAGATTCAATCGAATTGAAATCGCGAAATTTTTAATT
TCCAATAATTGTAACTTAGAAGCTCCCAATTATAAATACGACCGACCAAT
CCATATTGCCTGTGAATACGGATTTGAGGATATTTGCAGCTTATTAATCC
AGCAGAATTGTGATGTAAATGCTGAAAATTCCTACGGAAACCGGCCGATT
CATTTGGCTTGCATAAATGGGCATTTGAGAATTATCGAGCATCTCTTTAA
GGTTAAATGCGATTGGGACTCTGCAAATTATTGGAACATGCGGCCAATTG
AATATGCTTGCAAGCTTGGAGTCAAATCGATCGTCCAATTGCTATTGGAC
AATAATTGCAATACCAACAAAACTATTTACATGGAACATAAAGAGGTTTA
TCATTTGTTTTATAGTAAAAAAACTAAGCGAATAGATCGGTTCAAAATGC
ATATCGGTCATCACAAGGAAATTGAATCTCCCTTGTATGTCAGCTGTCAA
AGGGGACACCTTGATATTGTAGAGTTGATTCTCGACGCAAGACTAAGAAA
CGTGCGCAACCTTACCAATAATTGTCATTCCTCAGCAGAGGATATGATTA
AATGTCAATCATTGATTGACGATGAAAAAGAACGTTTCTGCACCCATATT
GCATGTTCCAGTGGCCACCTGAATGTTGTGCAATACCTATTAGACATCGG
GTGGTCGGATATCGAGTGTGAAGATATGAGCGGGGACAGGCTGATCCACA
TTGCCTGTTTGTTTTCACACGTTGATATCGTCCGGTTTCTTTTGGATCGG
AACTGTTGCTATGACATTATGAATAAGCGATATCAAAAGCCGATTCACAT
GATCCCTAGTTCAAGTTATGCCTCAATTCTGCCGCTGTTTCTCTCTAGGA
ATTGTTCTTTCGATCTCTCTGATGAATACAAATCAATTAACAGCTACAAA
AAATCCGAGAAACTCGAAAAGGCATTAAGAATAGAAAAGATTTTCTACTG
TATGGAAAATCGGATTTTCCCAGAATTAGAAAAGATGATTTTCCAATATT
ACTCACAATATAGATCGGAAATTATTTTCAGACTTTCCTGCTACTTTACA
GGCTCAATACAAATGACGAAACCTTCGAATTCTTTAAAGAAGATGTGCAA
ATTGATCTGCTTGATTGATAATGACAATCGGATAGTTGTGGGTAAAATTG
AGATAATTCCAGAATTTTACAACCGGAAACTGAATTCTCAAGCCAGGAAA
TATCAGAGGACGGAGAAAATCTGTCGAAAAATGTGCGAAATTTGCTTTAC
TAATGAAATCAATTACAGATTTCCGTGTGGACACGTAACTTGTAGCTATT
GCAGCTGGAACGCGGATTTTTGCTATTATTGCTGGGTCCCGAATAACGGA
GGCAAATTCATTCAGCAATAGATCAGCAGATTGCCATAAATAAATATGAT
TTGTTGACTTCAATGTTGTTAATTTCAATGTTTATTCTAAATATATATTA
TGTTTAGATATCACTGGAGCATTCTAGAGATCCTTGCGTTCGTCGGGTTT
TCTGCATTGCAGTCGGTAAGTTGATAACTAAATGAAATGGATCATTTGAG
ATGGAAACTTTGTTGAATATACTGTCGGATCAGGTCATGAAATTCGGGAA
ATTCATTATTTTTGATGTTTTTATTTTGGTATCAAAAAATGTCGGGTCCA
TCTTATGAGGCCTCAAGTCGGGTCAACAAGAGGATTTCCCCGTTCATTTG
TAGTTATATTTTGCAGCTCATGTCGGTTATAAGATTTTATTATTTAAAAT
TTAACTTTCTATTGAGCGTCATGCTCTTTTTTGTAAATAACTTATCTGGC
CCAGAATCGTTACTTTCTGTGGTTTTGAGCCTGGTGTGTATATTGAAACA
TTGGTACATGAATGATACATAGATCGGAGGATGTCAATTGTAACGGTCGA
GTGTATCAATATGTGGATTGATGCTACATCAATGGAAATATCAGAGGAAA
TTATGAAAACATTGGAACTACTATGATAATGATTATAGAGGAATGATCTA
TACAGTTGAATTGGGCGGTGATATATTTTTAAACAGTGTGTTATAGATTT
GCTTAAGAAAACAATTGAAGTAGAATTCAAAAAATCCAATTAGACGAAAG
TTTAAAGTAATGTCTTTTCACTTGGAAATCATTATAAGCAGCTCAGACGT
GATGGTTTGCAGATCATTTAACATGAAATTTACGAGTGGTAATGGCTATT
GACAAGAAGTGGCTGTATAGTGTTTCAGTGCCACATAACCCTGTGACTGG
GCATGAGCAGATCCCGATAATGATAGGCCAGAACAACGTCGTCATGCTAA
CCCAATATCAGCTAGTGTTAAAATATTAATAAAAATAATAAATTTTACCA
TTATTTTTTTTGTATAATTTGATAATTATTGTTGTTGTCACAATCATCAT
TGTATGAATTATTGCAGTATTTAGAGTATTT
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protein sequence of SMED30018624-orf-1

>SMED30018624-orf-1 ID=SMED30018624-orf-1|Name=SMED30018624-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=613bp
MEKENLLSQACLNGDLIEVLNLLTRKTTNIEEINENLYRPIHFAITNSNV
AIVELLISNKCDIEAENDQKMRPIHLACKYGNLDIVQLLISNGCDLSVND
LDGNSPSHLACEFGHLDIIKFLISNGSDLEARNNFSQYLIHNACRFNRIE
IAKFLISNNCNLEAPNYKYDRPIHIACEYGFEDICSLLIQQNCDVNAENS
YGNRPIHLACINGHLRIIEHLFKVKCDWDSANYWNMRPIEYACKLGVKSI
VQLLLDNNCNTNKTIYMEHKEVYHLFYSKKTKRIDRFKMHIGHHKEIESP
LYVSCQRGHLDIVELILDARLRNVRNLTNNCHSSAEDMIKCQSLIDDEKE
RFCTHIACSSGHLNVVQYLLDIGWSDIECEDMSGDRLIHIACLFSHVDIV
RFLLDRNCCYDIMNKRYQKPIHMIPSSSYASILPLFLSRNCSFDLSDEYK
SINSYKKSEKLEKALRIEKIFYCMENRIFPELEKMIFQYYSQYRSEIIFR
LSCYFTGSIQMTKPSNSLKKMCKLICLIDNDNRIVVGKIEIIPEFYNRKL
NSQARKYQRTEKICRKMCEICFTNEINYRFPCGHVTCSYCSWNADFCYYC
WVPNNGGKFIQQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0044325ion channel binding
GO:0051117ATPase binding
GO:0005200structural constituent of cytoskeleton
GO:0019899enzyme binding
GO:0019901protein kinase binding
GO:0030507spectrin binding
GO:0030674protein binding, bridging
Vocabulary: biological process
TermDefinition
GO:0036371protein localization to T-tubule
GO:0043268positive regulation of potassium ion transport
GO:0050821protein stabilization
GO:0051279regulation of release of sequestered calcium ion into cytosol
GO:0051597response to methylmercury
GO:0051924regulation of calcium ion transport
GO:0051928positive regulation of calcium ion transport
GO:0055117regulation of cardiac muscle contraction
GO:0060307regulation of ventricular cardiac muscle cell membrane repolarization
GO:0070296sarcoplasmic reticulum calcium ion transport
GO:0070972protein localization to endoplasmic reticulum
GO:0072659protein localization to plasma membrane
GO:0072661protein targeting to plasma membrane
GO:0086004regulation of cardiac muscle cell contraction
GO:0086005ventricular cardiac muscle cell action potential
GO:0086014atrial cardiac muscle cell action potential
GO:0086015SA node cell action potential
GO:0086046membrane depolarization during SA node cell action potential
GO:0086066atrial cardiac muscle cell to AV node cell communication
GO:0086070SA node cell to atrial cardiac muscle cell communication
GO:0086091regulation of heart rate by cardiac conduction
GO:0098907regulation of SA node cell action potential
GO:0098910regulation of atrial cardiac muscle cell action potential
GO:1901018positive regulation of potassium ion transmembrane transporter activity
GO:1901019regulation of calcium ion transmembrane transporter activity
GO:1901021positive regulation of calcium ion transmembrane transporter activity
GO:2001259positive regulation of cation channel activity
GO:0002027regulation of heart rate
GO:0003283atrial septum development
GO:0006874cellular calcium ion homeostasis
GO:0006888ER to Golgi vesicle-mediated transport
GO:0007010cytoskeleton organization
GO:0007165signal transduction
GO:0007399nervous system development
GO:0010628positive regulation of gene expression
GO:0010881regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
GO:0010882regulation of cardiac muscle contraction by calcium ion signaling
GO:0030913paranodal junction assembly
GO:0031647regulation of protein stability
GO:0033292T-tubule organization
GO:0033365protein localization to organelle
GO:0034394protein localization to cell surface
GO:0034613cellular protein localization
GO:0036309protein localization to M-band
Vocabulary: cellular component
TermDefinition
GO:0042383sarcolemma
GO:0043005neuron projection
GO:0043034costamere
GO:0045121membrane raft
GO:0045202synapse
GO:0045211postsynaptic membrane
GO:0005622intracellular
GO:0005737cytoplasm
GO:0005829cytosol
GO:0005856cytoskeleton
GO:0005886plasma membrane
GO:0014704intercalated disc
GO:0016020membrane
GO:0016323basolateral plasma membrane
GO:0016324apical plasma membrane
GO:0030018Z disc
GO:0030054cell junction
GO:0030315T-tubule
GO:0031430M band
GO:0031672A band
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 234..263
e-value: 150.0
score: 11.1
coord: 102..131
e-value: 6.3E-5
score: 32.4
coord: 296..326
e-value: 0.79
score: 18.8
coord: 69..98
e-value: 7.0E-4
score: 28.9
coord: 383..414
e-value: 13.0
score: 14.8
coord: 201..230
e-value: 0.31
score: 20.1
coord: 135..164
e-value: 13.0
score: 14.7
coord: 168..197
e-value: 0.067
score: 22.3
coord: 349..379
e-value: 3.6
score: 16.6
coord: 2..32
e-value: 1200.0
score: 4.3
coord: 36..65
e-value: 1.0
score: 18.4
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 69..101
score: 12.102
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 168..200
score: 9.297
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 36..68
score: 9.671
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 102..134
score: 14.025
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 383..415
score: 8.763
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 201..233
score: 9.137
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 289..468
e-value: 2.0E-21
score: 78.3
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 136..288
e-value: 5.7E-32
score: 112.6
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 2..135
e-value: 4.6E-35
score: 122.6
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 205..445
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 4..256
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 173..263
e-value: 8.6E-13
score: 48.7
coord: 349..411
e-value: 1.8E-8
score: 34.8
coord: 74..163
e-value: 1.2E-16
score: 61.1
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 2..448
score: 66.93
NoneNo IPR availablePANTHERPTHR24126FAMILY NOT NAMEDcoord: 99..263
coord: 299..416
coord: 5..166
coord: 140..325