SMED30018615

Overview
NameSMED30018615
Smed IDSMED30018615
Length (bp)1820
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018615 (SMED30018615) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018615 (SMED30018615) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018615 (SMED30018615) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018615 (SMED30018615) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018615

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30018615SMESG000035950.1 dd_Smed_v4_6080_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018615SMESG000035950.1 dd_Smed_v4_6080_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018615SMESG000035950.1 dd_Smed_v4_6080_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018615SMESG000035950.1 Contig48289newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018615SMESG000035950.1 Contig48289uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30018615SMESG000035950.1 dd_Smed_v4_6080_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30018615 vs. TrEMBL
Match: A0A5K3EHJ5 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.056e-7
Identity = 78/335 (23.28%), Postives = 139/335 (41.49%), Query Frame = 2 
Query:  215 MELYKFLPEFTGPNMLPEHRMKHYRDNIRPNMQYCGLAFLS--------KLNAWCEYIKEYDS---KPVPE---TPNKDXXXXXXXXXXXXXXXXXXXXXXAG-----CYDNYYCNVQSLSGIIHALMVAMAILSIM---GFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLM-LIIGALLLYTVPKFAEYTFNRN--FTTGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFI 1144
            M+ Y  LP  +    +P+  ++ Y +  +   +YC    L+        K   WC YI  + +    P P    +P     +N+ + S    ++   T    G     CYD+Y C       +    +V  AI +++   G L N  C     +  N S  R+   +Y + I  +DC++L +   L   + +    Y  Q+  Y + +   +P  +P    + ++  WLI+ +L  R N  +        G    +V S K+    + R+  ++IG    Y+VPKF EY    N    TG  +V   +    +YR +M   L+  +E F+P + +
Sbjct:   14 MDQYSSLPPVS---EIPKKPVQFYYEKAKKLRKYCKPELLNNEEIANYPKFKDWCNYITRFSTCNIAPHPTEFFSPLNSPVSNTASLSVVATSSAPATIGPQGVFGYSCYDDYLCEPADKGSLF--FLVYGAIFTLICSAGLLLNLACL----VGFNRSTNRSGATLYFSIICLMDCVYLGIKLALKSTVHLNDVDYSGQVNAYAKRAAYFIPTAVPLLTFSEVVSVWLIVCVLFDRYNYLQ-------RGYFSKSVTSLKR----HIRIASVVIGLSFCYSVPKFFEYKAEPNPAIPTGYRVVYTTVGNSTVYRSLMDYLLNIPLEMFLPYLAV 328
[Close]
BLAST of SMED30018615 vs. TrEMBL
Match: A0A4Z2DMT2 (FMRFamide receptor isoform X1 isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_010815 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.635e-7
Identity = 63/224 (28.12%), Postives = 107/224 (47.77%), Query Frame = 2 
Query:  476 AGCYDNYYCNVQSLSGIIHALM-VAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNRNFTTGKGIVIRLLRV----FKLYRIIMLKFLSPSIECFIP 1132
             GCYD+Y C+    S   H ++ V    L + G ++N   F      +N    R+   IYL+++   DC+ + L+ LL  V+  + S + +Q+++Y +FS  ++P  LP + +  L+V WL IALLV R       L+    G    ++ S  +       + L+  A   Y   KF EYT+       +  V+R+L       +++R IM  +L   +E F+P
Sbjct:  119 TGCYDDYICDKPYNSYPNHCILGVVCFTLCLCGIISNLVIF----PAYNFGLNRSGATIYLSAMALFDCIFMGLS-LLINVVHYLPSSFVEQMEMYGQFSGYLIPYGLPVFHICELLVVWLTIALLVNR-------LLYLKWGPHSKSLCSQVESVKIVTAIFLLCIA---YMGGKFFEYTY---VEYPEKKVVRVLFTQLGKMEMFRDIMDNWLKVPLEMFLP 324
[Close]
BLAST of SMED30018615 vs. TrEMBL
Match: A0A183P0P9 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS7935 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.663e-7
Identity = 61/225 (27.11%), Postives = 110/225 (48.89%), Query Frame = 2 
Query:  476 AGCYDNYYCNVQSLSGIIHALM-VAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLL---YTVPKFAEYTFNRNFTTGKGIVIRLLRVFK--LYRIIMLKFLSPSIECFIP 1132
             GCYD+Y C+ Q  S   H ++ V   IL + G ++N   F      +N    R+   +YL+++   DC+ + L+ L+ ++  +  S + +Q+++Y +FS  ++P  LP   +  L+V WL IALL+ R       L+    G    ++ S  +       + ++I    L   Y   KF EYT+   +   K + + L ++ K  ++R IM  +L   +E F+P
Sbjct:  119 TGCYDDYICDKQYNSYPNHCILGVVCFILCLCGIISNIVLF----PAYNFGLNRSGATVYLSAMAIFDCVFMGLSLLINVIHYLPPS-FIEQMEMYGQFSGYLIPYGLPVIQICELLVVWLTIALLINR-------LLYLNLGPHSKSLCSQLE------SVKIVIAVFFLCIAYMGCKFFEYTY-VEYPDKKVVRVLLTQLGKTEMFRDIMDNWLKVPLEMFLP 324
[Close]
BLAST of SMED30018615 vs. TrEMBL
Match: A0A4Z2DMQ2 (FMRFamide receptor isoform X1 isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_010815 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.219e-7
Identity = 42/129 (32.56%), Postives = 70/129 (54.26%), Query Frame = 2 
Query:  476 AGCYDNYYCNVQSLSGIIHALM-VAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQR 859
             GCYD+Y C+    S   H ++ V    L + G ++N   F      +N    R+   IYL+++   DC+ + L+ LL  V+  + S + +Q+++Y +FS  ++P  LP + +  L+V WL IALLV R
Sbjct:   71 TGCYDDYICDKPYNSYPNHCILGVVCFTLCLCGIISNLVIF----PAYNFGLNRSGATIYLSAMALFDCIFMGLS-LLINVVHYLPSSFVEQMEMYGQFSGYLIPYGLPVFHICELLVVWLTIALLVNR 194
[Close]
BLAST of SMED30018615 vs. TrEMBL
Match: A0A0R3UED9 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS5360 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 6.652e-7
Identity = 57/227 (25.11%), Postives = 100/227 (44.05%), Query Frame = 2 
Query:  482 CYDNYYCNVQSLSGIIHALMVAMAILSIM---GFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLM-LIIGALLLYTVPKFAEYTFNRN--FTTGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFI 1144
            CYD+Y C       +    +V  AI +++   G L N  C     +  N S  R+   +Y + I  +DC++L +   L   + +    Y  Q+  Y + +   +P  +P    + ++  WLI+ +L  R N  +        G    +V S K+    + R+  ++IG    Y+VPKF EY    N    TG  +V   +    +YR +M   L+  +E F+P + +
Sbjct:  127 CYDDYLCEPADKGSLF--FLVYGAIFTLICSAGLLLNLACL----VGFNRSTNRSGATLYFSIICLMDCVYLGIKLALKSTVHLNDVDYSGQVNAYAKRAAYFIPTAVPLLTFSEVVSVWLIVCVLFDRYNYLQ-------RGYFSKSVTSLKR----HIRIASVVIGLSFCYSVPKFFEYKAEPNPAIPTGYRVVYTTVGNSTVYRSLMDYLLNIPLEMFLPYLAV 336
[Close]
BLAST of SMED30018615 vs. Planmine SMEST
Match: SMESG000035950.1 (SMESG000035950.1)

HSP 1 Score: 867.455 bits (2240), Expect = 0.000e+0
Identity = 445/449 (99.11%), Postives = 448/449 (99.78%), Query Frame = 2 
Query:  215 MELYKFLPEFTGPNMLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYIKEYDSKPVPETPNKDXXXXXXXXXXXXXXXXXXXXXXAGCYDNYYCNVQSLSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNRNFTTGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQVLSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLEQSLEGSPKEEWPKLRQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI 1561
            MELYKFLPEFTGPNMLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYIKEYDSKPVPETPNKDFTANSNNQSQTTFTNNLLTDNDAGCYDNYYCNVQSLSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNR+F TGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQVLSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLE+SLEGSPKEEWP+LRQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI
Sbjct:    1 MELYKFLPEFTGPNMLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYIKEYDSKPVPETPNKDFTANSNNQSQTTFTNNLLTDNDAGCYDNYYCNVQSLSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNRDFRTGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQVLSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLEKSLEGSPKEEWPRLRQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI 449
[Close]
BLAST of SMED30018615 vs. Planmine SMEST
Match: SMESG000035950.1 (SMESG000035950.1)

HSP 1 Score: 836.639 bits (2160), Expect = 0.000e+0
Identity = 431/435 (99.08%), Postives = 434/435 (99.77%), Query Frame = 2 
Query:  257 MLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYIKEYDSKPVPETPNKDXXXXXXXXXXXXXXXXXXXXXXAGCYDNYYCNVQSLSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNRNFTTGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQVLSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLEQSLEGSPKEEWPKLRQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI 1561
            MLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYIKEYDSKPVPETPNKDFTANSNNQSQTTFTNNLLTDNDAGCYDNYYCNVQSLSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNR+F TGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQVLSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLE+SLEGSPKEEWP+LRQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI
Sbjct:    1 MLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYIKEYDSKPVPETPNKDFTANSNNQSQTTFTNNLLTDNDAGCYDNYYCNVQSLSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLMLIIGALLLYTVPKFAEYTFNRDFRTGKGIVIRLLRVFKLYRIIMLKFLSPSIECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQVLSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLEKSLEGSPKEEWPRLRQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI 435
[Close]
BLAST of SMED30018615 vs. Planmine SMEST
Match: SMESG000036502.1 (SMESG000036502.1)

HSP 1 Score: 68.1662 bits (165), Expect = 9.356e-12
Identity = 41/135 (30.37%), Postives = 70/135 (51.85%), Query Frame = 2 
Query:  476 AGCYDNYYCNVQSLS-------GIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASIIFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLTCLIVTWLIIALLVQR 859
              CYD+YYCN +S++       GI   +M  + IL+   FL  F          N++  R    +YL +I  +DC+++IL + + +V+  +   Y  QL +Y RF+  ++P  LP   L  L+  W+ +A+L+ R
Sbjct:  111 GTCYDDYYCNQRSVAPYLHYILGITCLIMCLIGILTTSLFLPVF----------NVASHRTGVTLYLTAIAIIDCIYMIL-FTVIVVIQYIPYEYTSQLDMYSRFTAHLIPHGLPTLQLMELLSVWITVAVLIDR 234
The following BLAST results are available for this feature:
BLAST of SMED30018615 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K3EHJ51.056e-723.28Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A4Z2DMT23.635e-728.13FMRFamide receptor isoform X1 isoform 1 OS=Schisto... [more]
A0A183P0P93.663e-727.11Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A4Z2DMQ24.219e-732.56FMRFamide receptor isoform X1 isoform 2 OS=Schisto... [more]
A0A0R3UED96.652e-725.11Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of SMED30018615 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018615 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000035950.10.000e+099.11SMESG000035950.1[more]
SMESG000035950.10.000e+099.08SMESG000035950.1[more]
SMESG000036502.19.356e-1230.37SMESG000036502.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018615 ID=SMED30018615|Name=SMED30018615|organism=Schmidtea mediterranea sexual|type=transcript|length=1820bp
ATCAAAAACTAAAACTAAGCTTTTAATATACTGTTACATCAAATTTATGA
CCTTGAAACAAAAAGCCAATCAAATCGTGAAAATATGGTGTGATACGAAT
AGTGATTAAGACTTGTAAATCATTATAGTAATCCAAGTACTATTTATAGA
CTATAATTACAAAGATTTATGTTTTAAGTTTTATTTTAATTGATATAATT
TTGTTAAAATCAAAATGGAATTATATAAATTTTTGCCCGAATTCACAGGG
CCTAATATGCTCCCAGAACACCGAATGAAACATTACAGGGATAACATCAG
GCCAAATATGCAATACTGTGGTTTGGCGTTTTTATCTAAACTTAATGCAT
GGTGTGAATACATTAAAGAGTATGATTCAAAACCTGTACCCGAAACGCCA
AATAAAGATTTTACTGCAAACTCAAATAACCAGAGCCAAACGACATTTAC
CAATAATTTGCTCACAGATAATGACGCCGGGTGTTATGATAATTACTATT
GTAACGTCCAATCCTTATCTGGTATCATTCACGCTTTAATGGTCGCCATG
GCCATCCTCAGTATCATGGGTTTCTTGGCGAACTTTTATTGTTTCTTTAA
AATGCATTTAAAACACAACATGAGCTTTAGAAGAAATCCTTTCGGAATAT
ATCTCGCTTCGATAATTTTCGTTGATTGTTTGCATCTCATTTTGGCCTGG
CTGCTATTTATTGTAATGGGAATAGTCGGAAGTCATTATAAAGATCAATT
AAAGATTTATCAAAGATTTTCCTATGTTATTGTGCCGGTTCTGTTACCGT
TCTGGGATTTGACATGTCTGATTGTCACCTGGCTTATTATTGCGTTATTA
GTTCAAAGAAGCAACATAACCAGGTGCAAACTGATCCAAGAAGCTGAAGG
TTGCGATGATTCGAATGTAATGAGTGCCAAAAAACGAAAGTGGACTTACT
ATAGATTAATGTTAATTATTGGTGCTCTGTTGCTGTATACTGTTCCAAAG
TTTGCAGAGTATACTTTCAATAGGAATTTCACAACTGGGAAAGGTATTGT
GATAAGGTTGCTTAGGGTTTTCAAATTATATCGAATAATCATGTTGAAAT
TTCTTTCACCAAGTATCGAATGTTTCATACCGATTATTTTCATTCTGGCC
TACATCGGTGTGATCAAATATCACGAGTCCGTTCTCACTCGCAGAAGAAT
GACTATATCGATGTCAAGCAAAACGCCAATTAGTGCCACTCTTATCAATC
CGAATCGGCAAGTGCTCAGTGTAGCTGGCGTAAGTAGAAAAACCCAATTG
GTCATCTGGATAGCTGTGCTTACGCTTATCAATTTGATAATGAAATTGTC
TGCAGTATTTGTTGGCTTAGAACAAAGTCTAGAAGGCAGTCCGAAAGAAG
AATGGCCGAAATTACGACAAATTGTGAAATATATGGCGATTATTTTCTCC
ATAGTGACTGTATCAGTCAAACCTCTGGTTTACGTACATTGCTGTCCGAG
ACTTTTACGAAAGGATTTGTCGGGACAGTTTATGAAAGACCTGGATTCAA
CAGAAGTAATATGATTTTCAACGAGAAACTATTCCATTTCATCACAATGT
ATATCAAAAGATAACTATTTCAAATGTCTCTTATTTTAATAATTTTTAAA
TGATTTTTTAAAAAGTTTTTATGATTTTCGTGAGAAAATTTTTTGTGTAT
GAAAATGTTTGAAACTTTTTGAAATTTGTTTCTGAAATATTTGTATGATC
TCTTATGATCTTATATCATCAATTAGGAATCTATTGATTACCGTAGAAAA
TTTATTTCATTATTTGTGAC
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protein sequence of SMED30018615-orf-1

>SMED30018615-orf-1 ID=SMED30018615-orf-1|Name=SMED30018615-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=450bp
MELYKFLPEFTGPNMLPEHRMKHYRDNIRPNMQYCGLAFLSKLNAWCEYI
KEYDSKPVPETPNKDFTANSNNQSQTTFTNNLLTDNDAGCYDNYYCNVQS
LSGIIHALMVAMAILSIMGFLANFYCFFKMHLKHNMSFRRNPFGIYLASI
IFVDCLHLILAWLLFIVMGIVGSHYKDQLKIYQRFSYVIVPVLLPFWDLT
CLIVTWLIIALLVQRSNITRCKLIQEAEGCDDSNVMSAKKRKWTYYRLML
IIGALLLYTVPKFAEYTFNRNFTTGKGIVIRLLRVFKLYRIIMLKFLSPS
IECFIPIIFILAYIGVIKYHESVLTRRRMTISMSSKTPISATLINPNRQV
LSVAGVSRKTQLVIWIAVLTLINLIMKLSAVFVGLEQSLEGSPKEEWPKL
RQIVKYMAIIFSIVTVSVKPLVYVHCCPRLLRKDLSGQFMKDLDSTEVI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 96..440
e-value: 4.8E-6
score: 27.7
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 110..337
e-value: 3.8369E-4
score: 39.9237
NoneNo IPR availableTMHMMTMhelixcoord: 188..210
NoneNo IPR availableTMHMMTMhelixcoord: 146..168
NoneNo IPR availableTMHMMTMhelixcoord: 243..260
NoneNo IPR availableTMHMMTMhelixcoord: 297..319
NoneNo IPR availableTMHMMTMhelixcoord: 404..426
NoneNo IPR availableTMHMMTMhelixcoord: 362..384
NoneNo IPR availableTMHMMTMhelixcoord: 103..125