Musashi

Overview
NameMusashi
Smed IDSMED30018588
Length (bp)2495
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Musashi (SMED30018588) t-SNE clustered cells

Violin plots show distribution of expression levels for Musashi (SMED30018588) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Musashi (SMED30018588) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Musashi (SMED30018588) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018588

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30018588h1SMcG0011284 SmedASXL_011563SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30018588h1SMcG0011284 dd_Smed_v6_8902_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ventral epidermis progenitor cellSMED30018588h1SMcG0011284 dd_Smed_v4_8902_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30018588h1SMcG0011284 DN311207smed_ncbi_20240424PMID:25356635
Cowles et al., 2014
whole organism asexual adult colorimetric in situ hybridization evidence
nervous systemSMED30018588h1SMcG0011284 DN311207ncbi_smed_estsPMID:25356635
Cowles et al., 2014
whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30018588h1SMcG0011284 DN311207smed_ncbi_20240424PMID:25356635
Cowles et al., 2014
whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30018588h1SMcG0011284 DN311207ncbi_smed_estsPMID:25356635
Cowles et al., 2014
whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Musashi vs. Ensembl Human
Match: MSI2 (musashi RNA binding protein 2 [Source:HGNC Symbol;Acc:HGNC:18585])

HSP 1 Score: 230.335 bits (586), Expect = 4.779e-68
Identity = 110/200 (55.00%), Postives = 139/200 (69.50%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Human
Match: MSI2 (musashi RNA binding protein 2 [Source:HGNC Symbol;Acc:HGNC:18585])

HSP 1 Score: 220.705 bits (561), Expect = 1.277e-65
Identity = 107/196 (54.59%), Postives = 135/196 (68.88%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            KMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 KMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 187          

HSP 2 Score: 78.9518 bits (193), Expect = 1.239e-15
Identity = 37/88 (42.05%), Postives = 56/88 (63.64%), Query Frame = 2
Query:  641 SSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            +S+  + + K+FIGG+S  T+   L +YFS+FG +  C +M D  T R RGFGFVTF +  S +KV     H+L++KT++ K A P+ 
Sbjct:    9 TSVMFSPSSKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRR 96          
BLAST of Musashi vs. Ensembl Human
Match: MSI1 (musashi RNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:7330])

HSP 1 Score: 223.402 bits (568), Expect = 3.812e-65
Identity = 105/213 (49.30%), Postives = 146/213 (68.54%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            +DP KMFIGGLS  T+ E +R+YF ++G++KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT +++++YF QFG ++   LM DK T+RHRGFGFVTFE+ED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:   17 HDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 203          
BLAST of Musashi vs. Ensembl Human
Match: MSI2 (musashi RNA binding protein 2 [Source:HGNC Symbol;Acc:HGNC:18585])

HSP 1 Score: 221.476 bits (563), Expect = 8.386e-65
Identity = 106/195 (54.36%), Postives = 134/195 (68.72%), Query Frame = 2
Query:  326 MFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            MFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:    1 MFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 169          

HSP 2 Score: 65.855 bits (159), Expect = 8.191e-11
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKI 562
            K+F+GGLS  T  E ++ YF ++G +++ M+M D  T R RGFGFVTF     V+KV     H +++K ++ K A P+++
Sbjct:   89 KIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEV 168          
BLAST of Musashi vs. Ensembl Human
Match: DAZAP1 (DAZ associated protein 1 [Source:HGNC Symbol;Acc:HGNC:2683])

HSP 1 Score: 167.933 bits (424), Expect = 5.655e-45
Identity = 80/195 (41.03%), Postives = 118/195 (60.51%), Query Frame = 2
Query:  320 GKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            GK+F+GGL  +T+ ET+R YF +YG++ + +IM+D  T +SRGFGFV F + + V  VL   PH LD + IDPK   PR ++                G+  G  SD    N+K+ K+F+GGI  N  + EL EYF +FG++    ++ D    R RGFGF+TFE+E S ++  ++HFHD+  K VE K+A P++
Sbjct:   10 GKLFVGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPK--------EGWQKGPRSD----NSKSNKIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRD 192          

HSP 2 Score: 63.929 bits (154), Expect = 4.978e-10
Identity = 30/82 (36.59%), Postives = 50/82 (60.98%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIES 568
            K+F+GG+        +R+YF+K+G + E +++ D   +R RGFGF+TF +  SV + +N   H +  KK++ K A PR  +S
Sbjct:  114 KIFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKS 195          
BLAST of Musashi vs. Ensembl Celegans
Match: msi-1 (MuSashI (Fly neural) family; Neural RNA-binding protein MSI-1 [Source:UniProtKB/TrEMBL;Acc:G5EFS2])

HSP 1 Score: 218.394 bits (555), Expect = 3.216e-64
Identity = 104/199 (52.26%), Postives = 138/199 (69.35%), Query Frame = 2
Query:  314 DPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            DPGKMFIGGLS  T+ E +RDYF ++G++ E M+MRDP TKR+RGFGF+TF++ SSV KVLN   H LD KKIDPKVA P++ ++                    KL        KTKKVFIGG+S  +T +++++YF  +G +E   LM DKAT RHRGFGFVTF++++ A+KVC+IHFH++N K VE KKA PKE++
Sbjct:   43 DPGKMFIGGLSWQTTAENLRDYFGRFGEVNECMVMRDPATKRARGFGFITFVDPSSVDKVLNNREHELDGKKIDPKVAFPKRTQA--------------------KLV------TKTKKVFIGGLSATSTLEDMKQYFETYGKVEDAMLMFDKATQRHRGFGFVTFDSDEVADKVCEIHFHEINGKMVECKKAQPKEVM 215          

HSP 2 Score: 81.6481 bits (200), Expect = 1.705e-16
Identity = 40/103 (38.83%), Postives = 61/103 (59.22%), Query Frame = 2
Query:  608 INGYSSGKLS----DSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            ++G+   +L+    D S  +    K+FIGG+S  TT + L +YF +FG +  C +M D AT R RGFGF+TF +  S +KV +   H+L+ K ++ K A PK 
Sbjct:   22 VDGHEEARLNADSDDGSHGSQDPGKMFIGGLSWQTTAENLRDYFGRFGEVNECMVMRDPATKRARGFGFITFVDPSSVDKVLNNREHELDGKKIDPKVAFPKR 124          
BLAST of Musashi vs. Ensembl Celegans
Match: hrp-1 (Heterogeneous nuclear ribonucleoprotein A1 [Source:UniProtKB/Swiss-Prot;Acc:Q22037])

HSP 1 Score: 145.976 bits (367), Expect = 4.487e-38
Identity = 71/194 (36.60%), Postives = 114/194 (58.76%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            K+F+GGL+  T+ + MR+++ ++G+I + ++MRDP TKRSRGFGFVTF   + V   + + PH +D K +DPK AVPR      KN                     S +N  TK++++ G+  + T+  L EYF+++G +   E+++DKAT + RGFGFVTF++ DS ++      H +N    + +K L K+
Sbjct:   24 KIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPR----DDKN--------------------RSESNVSTKRLYVSGVREDHTEDMLTEYFTKYGTVTKSEIILDKATQKPRGFGFVTFDDHDSVDQCVLQKSHMVNGHRCDVRKGLSKD 193          

HSP 2 Score: 73.559 bits (179), Expect = 1.113e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 2
Query:  638 DSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            D+SL     +K+F+GG+++NTT   + E++SQFG +    +M D  T R RGFGFVTF  +   +       H ++ KTV+ K+A+P++
Sbjct:   14 DASLEPENLRKIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPRD 102          
BLAST of Musashi vs. Ensembl Celegans
Match: hrp-1 (Heterogeneous nuclear ribonucleoprotein A1 [Source:UniProtKB/Swiss-Prot;Acc:Q22037])

HSP 1 Score: 145.976 bits (367), Expect = 4.487e-38
Identity = 71/194 (36.60%), Postives = 114/194 (58.76%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            K+F+GGL+  T+ + MR+++ ++G+I + ++MRDP TKRSRGFGFVTF   + V   + + PH +D K +DPK AVPR      KN                     S +N  TK++++ G+  + T+  L EYF+++G +   E+++DKAT + RGFGFVTF++ DS ++      H +N    + +K L K+
Sbjct:   24 KIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPR----DDKN--------------------RSESNVSTKRLYVSGVREDHTEDMLTEYFTKYGTVTKSEIILDKATQKPRGFGFVTFDDHDSVDQCVLQKSHMVNGHRCDVRKGLSKD 193          

HSP 2 Score: 73.559 bits (179), Expect = 1.113e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 2
Query:  638 DSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            D+SL     +K+F+GG+++NTT   + E++SQFG +    +M D  T R RGFGFVTF  +   +       H ++ KTV+ K+A+P++
Sbjct:   14 DASLEPENLRKIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPRD 102          
BLAST of Musashi vs. Ensembl Celegans
Match: hrp-1 (Heterogeneous nuclear ribonucleoprotein A1 [Source:UniProtKB/Swiss-Prot;Acc:Q22037])

HSP 1 Score: 145.976 bits (367), Expect = 4.588e-38
Identity = 71/194 (36.60%), Postives = 114/194 (58.76%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            K+F+GGL+  T+ + MR+++ ++G+I + ++MRDP TKRSRGFGFVTF   + V   + + PH +D K +DPK AVPR      KN                     S +N  TK++++ G+  + T+  L EYF+++G +   E+++DKAT + RGFGFVTF++ DS ++      H +N    + +K L K+
Sbjct:   24 KIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPR----DDKN--------------------RSESNVSTKRLYVSGVREDHTEDMLTEYFTKYGTVTKSEIILDKATQKPRGFGFVTFDDHDSVDQCVLQKSHMVNGHRCDVRKGLSKD 193          

HSP 2 Score: 73.559 bits (179), Expect = 1.123e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 2
Query:  638 DSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            D+SL     +K+F+GG+++NTT   + E++SQFG +    +M D  T R RGFGFVTF  +   +       H ++ KTV+ K+A+P++
Sbjct:   14 DASLEPENLRKIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPRD 102          
BLAST of Musashi vs. Ensembl Celegans
Match: hrp-1 (Heterogeneous nuclear ribonucleoprotein A1 [Source:UniProtKB/Swiss-Prot;Acc:Q22037])

HSP 1 Score: 145.976 bits (367), Expect = 4.588e-38
Identity = 71/194 (36.60%), Postives = 114/194 (58.76%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            K+F+GGL+  T+ + MR+++ ++G+I + ++MRDP TKRSRGFGFVTF   + V   + + PH +D K +DPK AVPR      KN                     S +N  TK++++ G+  + T+  L EYF+++G +   E+++DKAT + RGFGFVTF++ DS ++      H +N    + +K L K+
Sbjct:   24 KIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPR----DDKN--------------------RSESNVSTKRLYVSGVREDHTEDMLTEYFTKYGTVTKSEIILDKATQKPRGFGFVTFDDHDSVDQCVLQKSHMVNGHRCDVRKGLSKD 193          

HSP 2 Score: 73.559 bits (179), Expect = 1.123e-13
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 2
Query:  638 DSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            D+SL     +K+F+GG+++NTT   + E++SQFG +    +M D  T R RGFGFVTF  +   +       H ++ KTV+ K+A+P++
Sbjct:   14 DASLEPENLRKIFVGGLTSNTTDDLMREFYSQFGEITDIIVMRDPTTKRSRGFGFVTFSGKTEVDAAMKQRPHIIDGKTVDPKRAVPRD 102          
BLAST of Musashi vs. Ensembl Fly
Match: Rbp6 (gene:FBgn0260943 transcript:FBtr0075285)

HSP 1 Score: 226.483 bits (576), Expect = 1.927e-66
Identity = 109/211 (51.66%), Postives = 140/211 (66.35%), Query Frame = 2
Query:  305 ICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQR 937
            + NDPGKMFIGGLS  TSPE++RDYF +YGDI E M+M+DP T+RSRGFGFVTF + +SV KVL +  H LD KK+DPKVA PR+                                 +TKK+F+GG+S  TT ++++ YF QFG +E   LM DK T+RHRGFGFVTF++ED  +KVC+IHFH++NNK VE KKA PKE++    + K R
Sbjct:   24 VPNDPGKMFIGGLSWQTSPESLRDYFGRYGDISEAMVMKDPTTRRSRGFGFVTFSDPNSVDKVLTQGTHELDGKKVDPKVAFPRRAHPKMV--------------------------TRTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKVCEIHFHEINNKMVECKKAQPKEVMLPANLAKTR 208          
BLAST of Musashi vs. Ensembl Fly
Match: Rbp6 (gene:FBgn0260943 transcript:FBtr0333861)

HSP 1 Score: 226.098 bits (575), Expect = 2.769e-66
Identity = 109/211 (51.66%), Postives = 140/211 (66.35%), Query Frame = 2
Query:  305 ICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQR 937
            + NDPGKMFIGGLS  TSPE++RDYF +YGDI E M+M+DP T+RSRGFGFVTF + +SV KVL +  H LD KK+DPKVA PR+                                 +TKK+F+GG+S  TT ++++ YF QFG +E   LM DK T+RHRGFGFVTF++ED  +KVC+IHFH++NNK VE KKA PKE++    + K R
Sbjct:   24 VPNDPGKMFIGGLSWQTSPESLRDYFGRYGDISEAMVMKDPTTRRSRGFGFVTFSDPNSVDKVLTQGTHELDGKKVDPKVAFPRRAHPKMV--------------------------TRTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKVCEIHFHEINNKMVECKKAQPKEVMLPANLAKTR 208          
BLAST of Musashi vs. Ensembl Fly
Match: Rbp6 (gene:FBgn0260943 transcript:FBtr0113427)

HSP 1 Score: 228.794 bits (582), Expect = 6.750e-66
Identity = 109/211 (51.66%), Postives = 140/211 (66.35%), Query Frame = 2
Query:  305 ICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQR 937
            + NDPGKMFIGGLS  TSPE++RDYF +YGDI E M+M+DP T+RSRGFGFVTF + +SV KVL +  H LD KK+DPKVA PR+                                 +TKK+F+GG+S  TT ++++ YF QFG +E   LM DK T+RHRGFGFVTF++ED  +KVC+IHFH++NNK VE KKA PKE++    + K R
Sbjct:   24 VPNDPGKMFIGGLSWQTSPESLRDYFGRYGDISEAMVMKDPTTRRSRGFGFVTFSDPNSVDKVLTQGTHELDGKKVDPKVAFPRRAHPKMVT--------------------------RTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKVCEIHFHEINNKMVECKKAQPKEVMLPANLAKTR 208          
BLAST of Musashi vs. Ensembl Fly
Match: msi (gene:FBgn0011666 transcript:FBtr0333665)

HSP 1 Score: 202.216 bits (513), Expect = 1.290e-55
Identity = 97/213 (45.54%), Postives = 133/213 (62.44%), Query Frame = 2
Query:  317 PGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRT 955
            PGK+F+GGLS  TS + +++YF  +G + + +IM+DPVT+RSRGFGF+TF E  +V+KVL    H LD KKIDPK A P+                                  KTKK+F+GG+S +T+ +E++ YFSQFG +E   ++MD+ T RHRGFGFVTFENED  ++VC+IHFH + NK VE KKA PKE +     L Q+  ++ T
Sbjct:  135 PGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQAN---------------------------KTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGT 320          

HSP 2 Score: 77.7962 bits (190), Expect = 1.901e-14
Identity = 36/90 (40.00%), Postives = 54/90 (60.00%), Query Frame = 2
Query:  632 LSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPK 901
            LS  S ++    K+F+GG+S  T+  +L+EYF+ FG +    +M D  T R RGFGF+TF+   + EKV  +  H L+ K ++ K A PK
Sbjct:  125 LSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPK 214          

HSP 3 Score: 68.9366 bits (167), Expect = 1.109e-11
Identity = 33/84 (39.29%), Postives = 52/84 (61.90%), Query Frame = 2
Query:  308 CNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRK 559
             N   K+F+GG+S  TS E ++ YF ++G ++E +++ D  TKR RGFGFVTF     V +V     H + +KK++ K A P++
Sbjct:  220 ANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKE 303          
BLAST of Musashi vs. Ensembl Fly
Match: msi (gene:FBgn0011666 transcript:FBtr0084882)

HSP 1 Score: 202.216 bits (513), Expect = 2.105e-55
Identity = 97/213 (45.54%), Postives = 133/213 (62.44%), Query Frame = 2
Query:  317 PGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRT 955
            PGK+F+GGLS  TS + +++YF  +G + + +IM+DPVT+RSRGFGF+TF E  +V+KVL    H LD KKIDPK A P+                                  KTKK+F+GG+S +T+ +E++ YFSQFG +E   ++MD+ T RHRGFGFVTFENED  ++VC+IHFH + NK VE KKA PKE +     L Q+  ++ T
Sbjct:  174 PGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPKNRPRQAN---------------------------KTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKEAVTPAAQLLQKRIMLGT 359          

HSP 2 Score: 77.7962 bits (190), Expect = 1.808e-14
Identity = 36/90 (40.00%), Postives = 54/90 (60.00%), Query Frame = 2
Query:  632 LSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPK 901
            LS  S ++    K+F+GG+S  T+  +L+EYF+ FG +    +M D  T R RGFGF+TF+   + EKV  +  H L+ K ++ K A PK
Sbjct:  164 LSGGSGSDPAPGKLFVGGLSWQTSSDKLKEYFNMFGTVTDVLIMKDPVTQRSRGFGFITFQEPCTVEKVLKVPIHTLDGKKIDPKHATPK 253          

HSP 3 Score: 68.9366 bits (167), Expect = 1.198e-11
Identity = 33/84 (39.29%), Postives = 52/84 (61.90%), Query Frame = 2
Query:  308 CNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRK 559
             N   K+F+GG+S  TS E ++ YF ++G ++E +++ D  TKR RGFGFVTF     V +V     H + +KK++ K A P++
Sbjct:  259 ANKTKKIFVGGVSQDTSAEEVKAYFSQFGPVEETVMLMDQQTKRHRGFGFVTFENEDVVDRVCEIHFHTIKNKKVECKKAQPKE 342          
BLAST of Musashi vs. Ensembl Zebrafish
Match: msi2b (musashi RNA-binding protein 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1268])

HSP 1 Score: 230.335 bits (586), Expect = 1.201e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Zebrafish
Match: msi2b (musashi RNA-binding protein 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1268])

HSP 1 Score: 231.876 bits (590), Expect = 7.661e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Zebrafish
Match: msi2b (musashi RNA-binding protein 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1268])

HSP 1 Score: 221.476 bits (563), Expect = 1.099e-66
Identity = 107/196 (54.59%), Postives = 136/196 (69.39%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            KMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:    4 KMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 173          
BLAST of Musashi vs. Ensembl Zebrafish
Match: msi2a (musashi RNA-binding protein 2a [Source:ZFIN;Acc:ZDB-GENE-030826-25])

HSP 1 Score: 227.254 bits (578), Expect = 2.244e-66
Identity = 112/215 (52.09%), Postives = 143/215 (66.51%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRT 955
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGF+TF + SSV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S +T  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++   G   +   L  T
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFCKFGEIRECMVMRDPTTKRSRGFGFITFADVSSVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSASTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDIVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARSLPYT 206          
BLAST of Musashi vs. Ensembl Zebrafish
Match: msi2a (musashi RNA-binding protein 2a [Source:ZFIN;Acc:ZDB-GENE-030826-25])

HSP 1 Score: 227.254 bits (578), Expect = 2.244e-66
Identity = 112/215 (52.09%), Postives = 143/215 (66.51%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRT 955
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGF+TF + SSV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S +T  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++   G   +   L  T
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFCKFGEIRECMVMRDPTTKRSRGFGFITFADVSSVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSASTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDIVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARSLPYT 206          
BLAST of Musashi vs. Ensembl Xenopus
Match: kcnj4 (potassium inwardly rectifying channel subfamily J member 4 [Source:Xenbase;Acc:XB-GENE-493748])

HSP 1 Score: 227.254 bits (578), Expect = 5.023e-67
Identity = 109/200 (54.50%), Postives = 138/200 (69.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFE ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFNKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFEIEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Xenopus
Match: kcnj4 (potassium inwardly rectifying channel subfamily J member 4 [Source:Xenbase;Acc:XB-GENE-493748])

HSP 1 Score: 227.254 bits (578), Expect = 1.117e-66
Identity = 109/200 (54.50%), Postives = 138/200 (69.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFE ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFNKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFEIEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Xenopus
Match: msi1 (musashi RNA binding protein 1 [Source:NCBI gene;Acc:496961])

HSP 1 Score: 226.868 bits (577), Expect = 1.147e-66
Identity = 107/214 (50.00%), Postives = 145/214 (67.76%), Query Frame = 2
Query:  308 CNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
             +DP KMFIGGLS  T+ E +R+YF  +GD+KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT +++++YF QFG ++   LM DK T+RHRGFGFVTFE ED  EKVCDIHFH++NNK VE KKA PKE++   G ++ R++++
Sbjct:   16 AHDPCKMFIGGLSWQTTQEGLREYFSHFGDVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFEGEDIVEKVCDIHFHEINNKMVECKKAQPKEVMSPTGSVRGRSRVM 203          
BLAST of Musashi vs. Ensembl Xenopus
Match: msi1 (musashi RNA binding protein 1 [Source:NCBI gene;Acc:496961])

HSP 1 Score: 226.868 bits (577), Expect = 1.189e-66
Identity = 107/214 (50.00%), Postives = 145/214 (67.76%), Query Frame = 2
Query:  308 CNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
             +DP KMFIGGLS  T+ E +R+YF  +GD+KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT +++++YF QFG ++   LM DK T+RHRGFGFVTFE ED  EKVCDIHFH++NNK VE KKA PKE++   G ++ R++++
Sbjct:   16 AHDPCKMFIGGLSWQTTQEGLREYFSHFGDVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFEGEDIVEKVCDIHFHEINNKMVECKKAQPKEVMSPTGSVRGRSRVM 203          
BLAST of Musashi vs. Ensembl Xenopus
Match: kcnj4 (potassium inwardly rectifying channel subfamily J member 4 [Source:Xenbase;Acc:XB-GENE-493748])

HSP 1 Score: 228.024 bits (580), Expect = 1.679e-66
Identity = 109/200 (54.50%), Postives = 138/200 (69.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFE ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFNKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFEIEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Mouse
Match: Msi2 (musashi RNA-binding protein 2 [Source:MGI Symbol;Acc:MGI:1923876])

HSP 1 Score: 229.95 bits (585), Expect = 3.562e-68
Identity = 110/200 (55.00%), Postives = 139/200 (69.50%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Mouse
Match: Msi2 (musashi RNA-binding protein 2 [Source:MGI Symbol;Acc:MGI:1923876])

HSP 1 Score: 230.72 bits (587), Expect = 3.980e-68
Identity = 110/200 (55.00%), Postives = 139/200 (69.50%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Mouse
Match: Msi1 (musashi RNA-binding protein 1 [Source:MGI Symbol;Acc:MGI:107376])

HSP 1 Score: 222.631 bits (566), Expect = 5.394e-65
Identity = 105/213 (49.30%), Postives = 145/213 (68.08%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            +DP KMFIGGLS  T+ E +R+YF ++G++KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT ++++ YF QFG ++   LM DK T+RHRGFGFVTFE+ED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:   17 HDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKHYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 203          
BLAST of Musashi vs. Ensembl Mouse
Match: Msi1 (musashi RNA-binding protein 1 [Source:MGI Symbol;Acc:MGI:107376])

HSP 1 Score: 204.142 bits (518), Expect = 1.178e-58
Identity = 95/199 (47.74%), Postives = 134/199 (67.34%), Query Frame = 2
Query:  353 TSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            T+ E +R+YF ++G++KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT ++++ YF QFG ++   LM DK T+RHRGFGFVTFE+ED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:    5 TTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKHYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 177          

HSP 2 Score: 70.8626 bits (172), Expect = 1.164e-12
Identity = 32/72 (44.44%), Postives = 46/72 (63.89%), Query Frame = 2
Query:  689 STNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            S  TT++ L EYF QFG ++ C +M D  T R RGFGFVTF ++   +KV     H+L++KT++ K A P+ 
Sbjct:    2 SWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRR 73          

HSP 3 Score: 67.781 bits (164), Expect = 1.431e-11
Identity = 34/87 (39.08%), Postives = 54/87 (62.07%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNC 583
            K+F+GGLS  T+ E ++ YF ++G + + M+M D  T R RGFGFVTF     V+KV     H +++K ++ K A P+++ S T + 
Sbjct:   84 KIFVGGLSVNTTVEDVKHYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSA 170          
BLAST of Musashi vs. Ensembl Mouse
Match: Msi1 (musashi RNA-binding protein 1 [Source:MGI Symbol;Acc:MGI:107376])

HSP 1 Score: 200.675 bits (509), Expect = 3.818e-58
Identity = 94/196 (47.96%), Postives = 132/196 (67.35%), Query Frame = 2
Query:  362 ETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            E +R+YF ++G++KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT ++++ YF QFG ++   LM DK T+RHRGFGFVTFE+ED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:    7 EGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKHYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 176          

HSP 2 Score: 67.781 bits (164), Expect = 8.362e-12
Identity = 34/84 (40.48%), Postives = 53/84 (63.10%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESST 574
            K+F+GGLS  T+ E ++ YF ++G + + M+M D  T R RGFGFVTF     V+KV     H +++K ++ K A P+++ S T
Sbjct:   83 KIFVGGLSVNTTVEDVKHYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPT 166          
BLAST of Musashi vs. UniProt/SwissProt
Match: sp|Q96DH6|MSI2H_HUMAN (RNA-binding protein Musashi homolog 2 OS=Homo sapiens OX=9606 GN=MSI2 PE=1 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 2.295e-67
Identity = 110/200 (55.00%), Postives = 139/200 (69.50%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. UniProt/SwissProt
Match: sp|Q920Q6|MSI2H_MOUSE (RNA-binding protein Musashi homolog 2 OS=Mus musculus OX=10090 GN=Msi2 PE=1 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 2.784e-67
Identity = 110/200 (55.00%), Postives = 139/200 (69.50%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF + +SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. UniProt/SwissProt
Match: sp|Q9VVE5|MSIR6_DROME (RNA-binding protein Musashi homolog Rbp6 OS=Drosophila melanogaster OX=7227 GN=Rbp6 PE=2 SV=3)

HSP 1 Score: 226.483 bits (576), Expect = 1.932e-65
Identity = 109/211 (51.66%), Postives = 140/211 (66.35%), Query Frame = 2
Query:  305 ICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQR 937
            + NDPGKMFIGGLS  TSPE++RDYF +YGDI E M+M+DP T+RSRGFGFVTF + +SV KVL +  H LD KK+DPKVA PR+                                 +TKK+F+GG+S  TT ++++ YF QFG +E   LM DK T+RHRGFGFVTF++ED  +KVC+IHFH++NNK VE KKA PKE++    + K R
Sbjct:   24 VPNDPGKMFIGGLSWQTSPESLRDYFGRYGDISEAMVMKDPTTRRSRGFGFVTFSDPNSVDKVLTQGTHELDGKKVDPKVAFPRRAHPKMV--------------------------TRTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKVCEIHFHEINNKMVECKKAQPKEVMLPANLAKTR 208          
BLAST of Musashi vs. UniProt/SwissProt
Match: sp|O43347|MSI1H_HUMAN (RNA-binding protein Musashi homolog 1 OS=Homo sapiens OX=9606 GN=MSI1 PE=1 SV=1)

HSP 1 Score: 223.402 bits (568), Expect = 1.831e-64
Identity = 105/213 (49.30%), Postives = 146/213 (68.54%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            +DP KMFIGGLS  T+ E +R+YF ++G++KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT +++++YF QFG ++   LM DK T+RHRGFGFVTFE+ED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:   17 HDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 203          
BLAST of Musashi vs. UniProt/SwissProt
Match: sp|Q61474|MSI1H_MOUSE (RNA-binding protein Musashi homolog 1 OS=Mus musculus OX=10090 GN=Msi1 PE=1 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 3.773e-64
Identity = 105/213 (49.30%), Postives = 145/213 (68.08%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            +DP KMFIGGLS  T+ E +R+YF ++G++KE ++MRDP+TKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NTT ++++ YF QFG ++   LM DK T+RHRGFGFVTFE+ED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:   17 HDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSVNTTVEDVKHYFEQFGKVDDAMLMFDKTTNRHRGFGFVTFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 203          
BLAST of Musashi vs. TrEMBL
Match: B1Q3I9 (Musashi OS=Dugesia japonica OX=6161 GN=DjmlgC PE=2 SV=1)

HSP 1 Score: 737.643 bits (1903), Expect = 0.000e+0
Identity = 413/446 (92.60%), Postives = 430/446 (96.41%), Query Frame = 2
Query:  239 MTLEIACHDXXXXXXXXXXXXPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLIXXXXXXXXXXXXXXXXXXXRQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSXX-XXXXXXXXXXXXLDIYSTSDPNHITASLIQQQTSQQPHNATXXXXXXXXXXXXXXXXGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNN 1573
            MTLEIACHDESNSEYETEN+SP CNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTF ESSSVKKVLNKTPH LDSKKIDPKVAVPRK+E+STKNC++S+DC+GINGYS+GKL DS++ + KTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENED+AEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRN LIRTSLPLISNQLQNNYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAP+SL APVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGH+INGNVAGPVLYSPSTPLFMSTT N +    NNNCTLDIYS+SDPNHITASL QQQTSQQPHNATAMIIASNNNSQ NILNGVSVKNT+FSNGCDSVTQIAQHIPTAHYLGATNN
Sbjct:    1 MTLEIACHDESNSEYETENNSPTCNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFFESSSVKKVLNKTPHILDSKKIDPKVAVPRKVETSTKNCESSLDCIGINGYSTGKLGDSNMISTKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDTAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNHLIRTSLPLISNQLQNNYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAPESLSAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHIINGNVAGPVLYSPSTPLFMSTTNNRQCNQNNNNCTLDIYSSSDPNHITASLFQQQTSQQPHNATAMIIASNNNSQTNILNGVSVKNTLFSNGCDSVTQIAQHIPTAHYLGATNN 446          
BLAST of Musashi vs. TrEMBL
Match: A0A0A7M1Y4 (Musashi-like gene C (Fragment) OS=Schmidtea polychroa OX=50054 GN=mlgC PE=2 SV=1)

HSP 1 Score: 321.627 bits (823), Expect = 7.355e-102
Identity = 155/156 (99.36%), Postives = 155/156 (99.36%), Query Frame = 2
Query:  302 PICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMD 769
            PICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNN KTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMD
Sbjct:    3 PICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNTKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMD 158          

HSP 2 Score: 67.3958 bits (163), Expect = 2.468e-9
Identity = 32/80 (40.00%), Postives = 52/80 (65.00%), Query Frame = 2
Query:  668 KVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEI 907
            K+FIGG+S  T+ + + +YF ++G ++   +M D  T R RGFGFVTF    S +KV +   H L++K ++ K A+P++I
Sbjct:   10 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKI 89          
BLAST of Musashi vs. TrEMBL
Match: A0A3Q0KQN1 (Rna-binding protein musashi-related OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 7.766e-85
Identity = 135/218 (61.93%), Postives = 167/218 (76.61%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIG-NNGVLKQRNQLIRTSL 961
            NDPGKMFIGGLSPTT+ E +RDYF+KYG+++E+MIMRDP+TKRSRGFGF+TF +   V+KVL   PH LD KKIDPK+AVPRK   +TK                         + KTK+VFIGG++T TT +EL EYFSQFG LE+CELMMDK+T+RHRGFGFVTFE+E+SAEKVC+IHFHDL+NK VEAKKA+PKE++  NN ++KQR+Q IR  +
Sbjct:   75 NDPGKMFIGGLSPTTTSEGLRDYFQKYGELREYMIMRDPLTKRSRGFGFITFSDPVCVEKVLETAPHILDYKKIDPKLAVPRKSGQNTKV------------------------STKTKRVFIGGVATQTTNEELSEYFSQFGKLESCELMMDKSTNRHRGFGFVTFESEESAEKVCEIHFHDLHNKMVEAKKAVPKEVMSTNNSLIKQRHQFIRAPI 268          

HSP 2 Score: 70.0922 bits (170), Expect = 5.733e-9
Identity = 36/96 (37.50%), Postives = 56/96 (58.33%), Query Frame = 2
Query:  638 DSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGV 925
            +S+ ++    K+FIGG+S  TT + L +YF ++G L    +M D  T R RGFGF+TF +    EKV +   H L+ K ++ K A+P++   N  V
Sbjct:   69 ESTADSNDPGKMFIGGLSPTTTSEGLRDYFQKYGELREYMIMRDPLTKRSRGFGFITFSDPVCVEKVLETAPHILDYKKIDPKLAVPRKSGQNTKV 164          
BLAST of Musashi vs. TrEMBL
Match: G4V9P3 (Rna-binding protein musashi-related OS=Schistosoma mansoni OX=6183 GN=Smp_154730 PE=4 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 1.317e-84
Identity = 135/215 (62.79%), Postives = 166/215 (77.21%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIG-NNGVLKQRNQLIR 952
            NDPGKMFIGGLSPTT+ E +RDYF+KYG+++E+MIMRDP+TKRSRGFGF+TF +   V+KVL   PH LD KKIDPK+AVPRK   +TK                         + KTK+VFIGG++T TT +EL EYFSQFG LE+CELMMDK+T+RHRGFGFVTFE+E+SAEKVC+IHFHDL+NK VEAKKA+PKE++  NN ++KQR+Q IR
Sbjct:   75 NDPGKMFIGGLSPTTTSEGLRDYFQKYGELREYMIMRDPLTKRSRGFGFITFSDPVCVEKVLETAPHILDYKKIDPKLAVPRKSGQNTKV------------------------STKTKRVFIGGVATQTTNEELSEYFSQFGKLESCELMMDKSTNRHRGFGFVTFESEESAEKVCEIHFHDLHNKMVEAKKAVPKEVMSTNNSLIKQRHQFIR 265          

HSP 2 Score: 69.707 bits (169), Expect = 7.803e-9
Identity = 37/108 (34.26%), Postives = 60/108 (55.56%), Query Frame = 2
Query:  602 LGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGV 925
            L +    +  + +S+ ++    K+FIGG+S  TT + L +YF ++G L    +M D  T R RGFGF+TF +    EKV +   H L+ K ++ K A+P++   N  V
Sbjct:   57 LALTKRQTSLVIESTADSNDPGKMFIGGLSPTTTSEGLRDYFQKYGELREYMIMRDPLTKRSRGFGFITFSDPVCVEKVLETAPHILDYKKIDPKLAVPRKSGQNTKV 164          
BLAST of Musashi vs. TrEMBL
Match: A0A5K4ET69 (Rna-binding protein musashi-related OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 1.526e-84
Identity = 135/218 (61.93%), Postives = 167/218 (76.61%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIG-NNGVLKQRNQLIRTSL 961
            NDPGKMFIGGLSPTT+ E +RDYF+KYG+++E+MIMRDP+TKRSRGFGF+TF +   V+KVL   PH LD KKIDPK+AVPRK   +TK                         + KTK+VFIGG++T TT +EL EYFSQFG LE+CELMMDK+T+RHRGFGFVTFE+E+SAEKVC+IHFHDL+NK VEAKKA+PKE++  NN ++KQR+Q IR  +
Sbjct:   75 NDPGKMFIGGLSPTTTSEGLRDYFQKYGELREYMIMRDPLTKRSRGFGFITFSDPVCVEKVLETAPHILDYKKIDPKLAVPRKSGQNTKV------------------------STKTKRVFIGGVATQTTNEELSEYFSQFGKLESCELMMDKSTNRHRGFGFVTFESEESAEKVCEIHFHDLHNKMVEAKKAVPKEVMSTNNSLIKQRHQFIRAPI 268          

HSP 2 Score: 71.2478 bits (173), Expect = 2.454e-9
Identity = 37/108 (34.26%), Postives = 60/108 (55.56%), Query Frame = 2
Query:  602 LGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGV 925
            L +    +  + +S+ ++    K+FIGG+S  TT + L +YF ++G L    +M D  T R RGFGF+TF +    EKV +   H L+ K ++ K A+P++   N  V
Sbjct:   57 LALTKRQTSLVIESTADSNDPGKMFIGGLSPTTTSEGLRDYFQKYGELREYMIMRDPLTKRSRGFGFITFSDPVCVEKVLETAPHILDYKKIDPKLAVPRKSGQNTKV 164          
BLAST of Musashi vs. Ensembl Cavefish
Match: msi2a (RNA-binding protein Musashi homolog 2-like [Source:NCBI gene;Acc:103045937])

HSP 1 Score: 230.72 bits (587), Expect = 1.480e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Cavefish
Match: msi2b (RNA-binding protein Musashi homolog 2 [Source:NCBI gene;Acc:103023867])

HSP 1 Score: 232.646 bits (592), Expect = 2.080e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Cavefish
Match: msi2b (RNA-binding protein Musashi homolog 2 [Source:NCBI gene;Acc:103023867])

HSP 1 Score: 232.261 bits (591), Expect = 2.195e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Cavefish
Match: msi2a (RNA-binding protein Musashi homolog 2-like [Source:NCBI gene;Acc:103045937])

HSP 1 Score: 231.876 bits (590), Expect = 3.764e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Cavefish
Match: msi2a (RNA-binding protein Musashi homolog 2-like [Source:NCBI gene;Acc:103045937])

HSP 1 Score: 231.491 bits (589), Expect = 5.550e-68
Identity = 110/200 (55.00%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG +E   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADAASVDKVLAQPHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Sea Lamprey
Match: HNRNPA0 (heterogeneous nuclear ribonucleoprotein A0 [Source:HGNC Symbol;Acc:HGNC:5030])

HSP 1 Score: 145.591 bits (366), Expect = 5.289e-40
Identity = 74/194 (38.14%), Postives = 112/194 (57.73%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            K+FIGGLS  T+ ++MR +F ++G + + ++MRDP TKRSRGFGFVT+     V   ++  PH LD++ ++ K A+ R+            D L    +             K KK+F+GGI  +T +Q L EYF  +G +E  E+M D++T + RGFGFVTF + D+ +KV    +H +N    E +KAL K+
Sbjct:   10 KLFIGGLSFETTEDSMRKHFEQWGTLTDCVVMRDPHTKRSRGFGFVTYTCPEEVDAAMDARPHHLDNRVVEAKRAISRE------------DSLKPGAH------------LKVKKIFVGGIRDDTDEQHLREYFQVYGDVEAAEVMEDRSTGKKRGFGFVTFSDHDTVDKVIVQKYHTINGHKAEVRKALSKQ 179          

HSP 2 Score: 74.7146 bits (182), Expect = 2.131e-15
Identity = 34/84 (40.48%), Postives = 53/84 (63.10%), Query Frame = 2
Query:  653 NAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            + + +K+FIGG+S  TT+  + ++F Q+G L  C +M D  T R RGFGFVT+   +  +   D   H L+N+ VEAK+A+ +E
Sbjct:    5 DEQQRKLFIGGLSFETTEDSMRKHFEQWGTLTDCVVMRDPHTKRSRGFGFVTYTCPEEVDAAMDARPHHLDNRVVEAKRAISRE 88          
BLAST of Musashi vs. Ensembl Sea Lamprey
Match: HNRNPA0 (heterogeneous nuclear ribonucleoprotein A0 [Source:HGNC Symbol;Acc:HGNC:5030])

HSP 1 Score: 147.902 bits (372), Expect = 4.134e-39
Identity = 72/194 (37.11%), Postives = 114/194 (58.76%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            K+FIGGLS  T+ E+M+ +F ++G + + ++MRDP TKRSRGFGFVT++ +  V   +   PH LD + ++ K A+ R+            D L    +             K KK+F+GGI  NT +  L +YFS++G++E+ ++M D+ + + RGFGFVTF + D+ +KV    +H +N    E +KAL K+
Sbjct:   34 KLFIGGLSFETTEESMQKHFEQWGTLTDCVVMRDPHTKRSRGFGFVTYMSAEEVDAAMEVRPHHLDGRVVEAKRAISRE------------DSLKPGAH------------LKVKKIFVGGIKDNTDEDHLRDYFSEYGMVESVDVMEDRQSGKKRGFGFVTFSDHDAVDKVVVQKYHTINGHKAEVRKALSKQ 203          

HSP 2 Score: 77.411 bits (189), Expect = 2.428e-15
Identity = 32/84 (38.10%), Postives = 56/84 (66.67%), Query Frame = 2
Query:  653 NAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            + + +K+FIGG+S  TT++ ++++F Q+G L  C +M D  T R RGFGFVT+ + +  +   ++  H L+ + VEAK+A+ +E
Sbjct:   29 DEQQRKLFIGGLSFETTEESMQKHFEQWGTLTDCVVMRDPHTKRSRGFGFVTYMSAEEVDAAMEVRPHHLDGRVVEAKRAISRE 112          
BLAST of Musashi vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004893.1 (pep scaffold:Pmarinus_7.0:GL479484:4:2056:-1 gene:ENSPMAG00000004449.1 transcript:ENSPMAT00000004893.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 138.658 bits (348), Expect = 2.978e-37
Identity = 70/200 (35.00%), Postives = 113/200 (56.50%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNG 922
            K+F+GGLS  T+ E +R YF ++G + + ++M+D  +KRSRGFGFVTF   S V   ++  PH +D + ++PK AV R+ +S       S+                       KK+F+GGI  +T ++ L +YF ++G +E  E+M ++ + + RGF FVTF + D  +K+    +H +N    E +KALPK+ + N G
Sbjct:   12 KLFVGGLSFETTEEGLRQYFEQWGSLTDCVVMKDTQSKRSRGFGFVTFSSMSEVDAAMSARPHKIDGRMVEPKRAVSRE-DSMRPGAHLSV-----------------------KKIFVGGIKDDTDEEHLRDYFEKYGRIEIVEVMTERESGKKRGFAFVTFNDHDPVDKIVVQKYHTINGHNCEIRKALPKQELQNYG 187          

HSP 2 Score: 68.9366 bits (167), Expect = 2.629e-13
Identity = 31/80 (38.75%), Postives = 49/80 (61.25%), Query Frame = 2
Query:  665 KKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            +K+F+GG+S  TT++ L +YF Q+G L  C +M D  + R RGFGFVTF +    +       H ++ + VE K+A+ +E
Sbjct:   11 RKLFVGGLSFETTEEGLRQYFEQWGSLTDCVVMKDTQSKRSRGFGFVTFSSMSEVDAAMSARPHKIDGRMVEPKRAVSRE 90          
BLAST of Musashi vs. Ensembl Sea Lamprey
Match: HNRNPA2B1 (heterogeneous nuclear ribonucleoprotein A2/B1 [Source:HGNC Symbol;Acc:HGNC:5033])

HSP 1 Score: 136.346 bits (342), Expect = 2.504e-36
Identity = 69/193 (35.75%), Postives = 111/193 (57.51%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPK 901
            K+FIGGLS  T+ E++R+Y+ ++G I + ++M+DP +KRSRGFGF+T+     V   +   PH +D + ++ K AVPR         D S   + +                  KK+F+GGI  NT +  L +YFS+FG +E+ +++ DK T + RGF FVTF++ D  +++    FH +N   +E +KAL +
Sbjct:   15 KLFIGGLSFETTEESLREYYEQWGTIVDCVVMKDPSSKRSRGFGFITYSVMEEVNSAMQARPHTVDGRVVEAKRAVPR---------DDSRPGIQVT----------------VKKLFVGGIKDNTDESHLRDYFSKFGRVESVDMVTDKQTGKKRGFAFVTFDDYDPVDQIVFQKFHTINCHKLEVRKALSR 182          

HSP 2 Score: 72.0182 bits (175), Expect = 3.113e-14
Identity = 30/82 (36.59%), Postives = 52/82 (63.41%), Query Frame = 2
Query:  659 KTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            + +K+FIGG+S  TT++ L EY+ Q+G +  C +M D ++ R RGFGF+T+   +          H ++ + VEAK+A+P++
Sbjct:   12 QMRKLFIGGLSFETTEESLREYYEQWGTIVDCVVMKDPSSKRSRGFGFITYSVMEEVNSAMQARPHTVDGRVVEAKRAVPRD 93          
BLAST of Musashi vs. Ensembl Sea Lamprey
Match: RBM39 (RNA binding motif protein 39 [Source:HGNC Symbol;Acc:HGNC:15923])

HSP 1 Score: 74.7146 bits (182), Expect = 3.524e-14
Identity = 45/181 (24.86%), Postives = 85/181 (46.96%), Query Frame = 2
Query:  302 PICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCD 844
            P   D   +F   L+    P  + ++F   G +++  ++ D  ++RS+G  ++ F+E  SV   +  T   L        + VP  +++S    + +         ++   +    + A   ++++G +  N T+  L+  F  FG +E C+LMMD  T R RG+GFVTF + + A K  +
Sbjct:  153 PEERDARTVFCMQLAARIRPRDLEEFFSAVGKVRDVRMISDRNSRRSKGIAYIEFVEVDSVSLAIGLTGQRL--------LGVPIIVQASQAEKNRAA-------AAAAAAATMQKSTAGPMRLYVGSLHFNITEDMLKGIFEPFGKIENCQLMMDSDTGRSRGYGFVTFADAECARKALE 318          

HSP 2 Score: 48.9062 bits (115), Expect = 3.657e-6
Identity = 31/116 (26.72%), Postives = 56/116 (48.28%), Query Frame = 2
Query:  317 PGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTS----------IDCLGINGYSSGKL 634
            P ++++G L    + + ++  F  +G I+   +M D  T RSRG+GFVTF ++   +K L +   F  + +       P K+   T+  D S          ++  GI+  S+G+L
Sbjct:  258 PMRLYVGSLHFNITEDMLKGIFEPFGKIENCQLMMDSDTGRSRGYGFVTFADAECARKALEQLNGFELAGR-------PMKVGHVTERSDGSGASSFLDNDELERTGIDLGSTGRL 366          
BLAST of Musashi vs. Ensembl Yeast
Match: HRP1 (Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability [Source:SGD;Acc:S000005483])

HSP 1 Score: 160.614 bits (405), Expect = 2.475e-42
Identity = 79/195 (40.51%), Postives = 116/195 (59.49%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEI 907
            KMFIGGL+  T+ + +R+YF KYG + +  IM+DP T RSRGFGF++F + SSV +V+ KT H LD K IDPK A+PR  +                               KT K+F+GGI  +   +E EE+FSQ+G +   +LM+DK T + RGFGFVT+++ D+ ++VC   F D  ++ +E K+A P+ +
Sbjct:  160 KMFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVV-KTQHILDGKVIDPKRAIPRDEQD------------------------------KTGKIFVGGIGPDVRPKEFEEFFSQWGTIIDAQLMLDKDTGQSRGFGFVTYDSADAVDRVCQNKFIDFKDRKIEIKRAEPRHM 323          

HSP 2 Score: 64.6994 bits (156), Expect = 3.462e-11
Identity = 33/86 (38.37%), Postives = 53/86 (61.63%), Query Frame = 2
Query:  320 GKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKV-LNKTPHFLDSKKIDPKVAVPRKIESST 574
            GK+F+GG+ P   P+   ++F ++G I +  +M D  T +SRGFGFVT+  + +V +V  NK   F D +KI+ K A PR ++  +
Sbjct:  243 GKIFVGGIGPDVRPKEFEEFFSQWGTIIDAQLMLDKDTGQSRGFGFVTYDSADAVDRVCQNKFIDFKD-RKIEIKRAEPRHMQQKS 327          
BLAST of Musashi vs. Ensembl Yeast
Match: NSR1 (Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation [Source:SGD;Acc:S000003391])

HSP 1 Score: 65.0846 bits (157), Expect = 1.958e-11
Identity = 46/177 (25.99%), Postives = 82/177 (46.33%), Query Frame = 2
Query:  314 DPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCD 844
            +P  +F+G LS +   E ++  F   G +    ++ +  T RSRG+G+V F   S  +K + +    +  K+ID +            NCD S      N   + K  D+    + T  +F+G +S N  +  + E F++ G + +  +     T + +GFG+V F N + A+K  D
Sbjct:  166 EPATIFVGRLSWSIDDEWLKKEFEHIGGVIGARVIYERGTDRSRGYGYVDFENKSYAEKAIQE----MQGKEIDGRPI----------NCDMSTSKPAGNNDRAKKFGDTPSEPSDT--LFLGNLSFNADRDAIFELFAKHGEVVSVRIPTHPETEQPKGFGYVQFSNMEDAKKALD 326          
BLAST of Musashi vs. Ensembl Yeast
Match: PAB1 (Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex [Source:SGD;Acc:S000000967])

HSP 1 Score: 62.7734 bits (151), Expect = 1.338e-10
Identity = 53/204 (25.98%), Postives = 92/204 (45.10%), Query Frame = 2
Query:  320 GKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGING-------YSSGKLSDSSLNNAKTK--KVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFE-NEDSAEKVCDIHFHDLNNKTVEAKKALPK 901
            G +FI  L P    + + D F  +GDI    I  D   K S+GFGFV F E  + K+ ++                              +++ + +NG       + S K  DS L   K     +++  I++ TT ++ +E F++FG + +  L  D A  + +GFGFV +E +ED+ + V  ++  +LN + +   +A  K
Sbjct:  126 GNIFIKNLHPDIDNKALYDTFSVFGDILSSKIATDENGK-SKGFGFVHFEEEGAAKEAID------------------------------ALNGMLLNGQEIYVAPHLSRKERDSQLEETKAHYTNLYVKNINSETTDEQFQELFAKFGPIVSASLEKD-ADGKLKGFGFVNYEKHEDAVKAVEALNDSELNGEKLYVGRAQKK 297          
BLAST of Musashi vs. Ensembl Yeast
Match: PUB1 (Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004961])

HSP 1 Score: 56.6102 bits (135), Expect = 9.037e-9
Identity = 45/194 (23.20%), Postives = 82/194 (42.27%), Query Frame = 2
Query:  326 MFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCD-IHFHDLNNKTVEAKKALPKE 904
            +++G L    + + ++ YF+  G I    IM D   K    + FV + +S      L      L+ K+I+  +    KI  + ++  +S              SD + N      +F+G ++ N   + L   F  F    +  +M D  T   RG+GFV+F ++D A+   D +   DLN + +    A  ++
Sbjct:   77 LYVGNLDKAITEDILKQYFQVGGPIANIKIMIDKNNKNVN-YAFVEYHQSHDANIALQT----LNGKQIENNIV---KINWAFQSQQSS--------------SDDTFN------LFVGDLNVNVDDETLRNAFKDFPSYLSGHVMWDMQTGSSRGYGFVSFTSQDDAQNAMDSMQGQDLNGRPLRINWAAKRD 242          
BLAST of Musashi vs. Ensembl Yeast
Match: SGN1 (Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation [Source:SGD;Acc:S000001440])

HSP 1 Score: 50.0618 bits (118), Expect = 4.719e-7
Identity = 33/130 (25.38%), Postives = 65/130 (50.00%), Query Frame = 2
Query:  524 KKIDPKVAVPRKIESSTKN------CDTSIDCLGING--YSSGKLSDSSLN----NAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTV 877
            +K+D K  +  +I ++ KN       D  +  L I G    S KLS    +     A ++ +F+G I+ + T +++E++F   G ++   L+ D+ T   +G+G++ FE+    EK   ++  +L  K +
Sbjct:    5 EKVDAKATLKTEISNNKKNDKQELELDELVGKLSIEGTPQVSQKLSKEEKHAHQLEADSRSIFVGNITPDVTPEQIEDHFKDCGQIKRITLLYDRNTGTPKGYGYIEFESPAYREKALQLNGGELKGKKI 134          

HSP 2 Score: 48.1358 bits (113), Expect = 2.078e-6
Identity = 26/82 (31.71%), Postives = 42/82 (51.22%), Query Frame = 2
Query:  314 DPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRK 559
            D   +F+G ++P  +PE + D+F+  G IK   ++ D  T   +G+G++ F   +  +K L      L  KKI    AV RK
Sbjct:   62 DSRSIFVGNITPDVTPEQIEDHFKDCGQIKRITLLYDRNTGTPKGYGYIEFESPAYREKALQLNGGELKGKKI----AVSRK 139          
BLAST of Musashi vs. Ensembl Nematostella
Match: EDO39879 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8S5])

HSP 1 Score: 200.29 bits (508), Expect = 1.040e-59
Identity = 101/196 (51.53%), Postives = 133/196 (67.86%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            KMFIGGLS  T+ E +R +F K+G++KE ++MRDPVTKRSRGFGF+TF +  +V  VLN     LD KKIDPK+AVP++  +  K   T                        TKK+FIGG+STNT+++++++YFSQFG +    LM DKAT RHRGFGFVTFE+E+SA+K CD  +H +NNK VE KKA PKE++
Sbjct:    1 KMFIGGLSWQTTAEGLRQHFEKFGELKECVVMRDPVTKRSRGFGFLTFKDPKAVDVVLNSGAQELDGKKIDPKLAVPKR--APAKMVTT------------------------TKKIFIGGLSTNTSEEDMKKYFSQFGKITETMLMFDKATQRHRGFGFVTFESENSADKACDTQYHLINNKKVEVKKAQPKEVM 170          
BLAST of Musashi vs. Ensembl Nematostella
Match: EDO33389 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSE1])

HSP 1 Score: 154.451 bits (389), Expect = 1.734e-43
Identity = 79/185 (42.70%), Postives = 114/185 (61.62%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTV 877
            K+F+GGL+ +T    ++ YF KYG++ + ++M DPVTKRSR FGF+TF + S V+ V++  PH LD K IDPK AVPR                   G   G    +  N     K+F+GG++ +T+KQ+LE+YF+ +G + +  LM+D  T + RGFGFVTFE++DS  K  D H+H +N KTV
Sbjct:    2 KIFVGGLAISTDSAGLQGYFEKYGEVTDCVLMTDPVTKRSRCFGFITFRDPSVVQNVMDDKPHQLDGKMIDPKPAVPR-------------------GPGQGPPQGNQNNEF---KIFVGGLAHSTSKQDLEDYFTAYGKVVSVNLMVDPQTQKMRGFGFVTFESKDSVTKAADEHYHQINGKTV 164          

HSP 2 Score: 67.0106 bits (162), Expect = 6.898e-13
Identity = 29/79 (36.71%), Postives = 47/79 (59.49%), Query Frame = 2
Query:  665 KKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPK 901
            +K+F+GG++ +T    L+ YF ++G +  C LM D  T R R FGF+TF +    + V D   H L+ K ++ K A+P+
Sbjct:    1 RKIFVGGLAISTDSAGLQGYFEKYGEVTDCVLMTDPVTKRSRCFGFITFRDPSVVQNVMDDKPHQLDGKMIDPKPAVPR 79          

HSP 3 Score: 63.929 bits (154), Expect = 7.112e-12
Identity = 30/74 (40.54%), Postives = 46/74 (62.16%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKI 532
            N+  K+F+GGL+ +TS + + DYF  YG +    +M DP T++ RGFGFVTF    SV K  ++  H ++ K +
Sbjct:   91 NNEFKIFVGGLAHSTSKQDLEDYFTAYGKVVSVNLMVDPQTQKMRGFGFVTFESKDSVTKAADEHYHQINGKTV 164          
BLAST of Musashi vs. Ensembl Nematostella
Match: EDO38417 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCX6])

HSP 1 Score: 137.887 bits (346), Expect = 4.993e-36
Identity = 72/201 (35.82%), Postives = 114/201 (56.72%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKT-PHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQ-FGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEI 907
            +D GK+F+GGLS  T+ E++++YF KYG++    I  D +T R RGF FV F   S    VLN+  PH LD + IDPK A P                        GK       + + KK+F+GG+   T+ +++ EYF + +  ++  E + + +++R RGF FV+F++ED+ +K+C+  FH++    VE K+ALPKE+
Sbjct:   26 DDIGKLFVGGLSYETTKESLKEYFSKYGELVGVDIKMDALTGRPRGFAFVQFKHQSEADAVLNQVDPHVLDGRTIDPKPAAP-----------------------IGKPP-----HLRVKKIFVGGLKPETSDEKIREYFGKAYAPVKEIEYITEHSSNRRRGFCFVSFDSEDTVDKICETQFHNIEGNKVEVKRALPKEV 198          

HSP 2 Score: 72.0182 bits (175), Expect = 1.114e-13
Identity = 40/106 (37.74%), Postives = 64/106 (60.38%), Query Frame = 2
Query:  608 INGYSSGKLSDSSLNNAKTK--------KVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCD-IHFHDLNNKTVEAKKALP 898
            +NGY       +SL++ KT+        K+F+GG+S  TTK+ L+EYFS++G L   ++ MD  T R RGF FV F+++  A+ V + +  H L+ +T++ K A P
Sbjct:    7 VNGYIG-----TSLDSNKTRMTKDDDIGKLFVGGLSYETTKESLKEYFSKYGELVGVDIKMDALTGRPRGFAFVQFKHQSEADAVLNQVDPHVLDGRTIDPKPAAP 107          
BLAST of Musashi vs. Ensembl Nematostella
Match: EDO49349 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG33])

HSP 1 Score: 127.872 bits (320), Expect = 5.601e-34
Identity = 76/201 (37.81%), Postives = 116/201 (57.71%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIM-RDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQF----GILETCELMMDKATS---RHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPK 901
            K+FIGGL+  T+ E ++DYF ++G I + +IM RD    RSRGFGFVT+  S SV +VL K  H LD ++I+PK +VPR                      SG     S    KT+K+F+GG+++ T +++++EYF+      G+ E  ++ + +      R RGF FVTF+N++  EKVC + +H++  K  E KKA P+
Sbjct:    1 KIFIGGLNWNTTEEGLKDYFSQWGTIVDCVIMKRDG---RSRGFGFVTYESSDSVNEVLKKKDHVLDDREIEPKRSVPRD--------------------ESGAPEAMS----KTRKIFVGGLASTTVEEDIKEYFNSLCRKNGMGEVIDVDLKRDRDNPKRIRGFAFVTFDNDEIVEKVCAMKYHEIRMKQCEVKKAEPQ 174          

HSP 2 Score: 75.0998 bits (183), Expect = 1.263e-15
Identity = 35/82 (42.68%), Postives = 57/82 (69.51%), Query Frame = 2
Query:  668 KVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIG 913
            K+FIGG++ NTT++ L++YFSQ+G +  C +M  K   R RGFGFVT+E+ DS  +V     H L+++ +E K+++P++  G
Sbjct:    1 KIFIGGLNWNTTEEGLKDYFSQWGTIVDCVIM--KRDGRSRGFGFVTYESSDSVNEVLKKKDHVLDDREIEPKRSVPRDESG 80          
BLAST of Musashi vs. Ensembl Nematostella
Match: EDO36522 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF0])

HSP 1 Score: 124.79 bits (312), Expect = 1.043e-32
Identity = 70/206 (33.98%), Postives = 115/206 (55.83%), Query Frame = 2
Query:  311 NDP-GKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKT----PHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQF--GILETCELMMDKA-TSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            NDP  K+F+GGL+  T+ ET+R+YF  YG++ + +++ D  TK+SRGFG+VTF +    + VL        H +D K+++ K A+PR   S+T                         ++ KTKK+F+GG+  + TK++++E         ++  +L+M K   ++HRGF FV   NED A+++C +    +  K VEAKKA P++
Sbjct:   10 NDPRAKLFVGGLNRETTNETLREYFEAYGELTDVVVICDSATKKSRGFGYVTFADYKVTRNVLKDKVENGAHRIDGKEVEVKRAIPRDDNSAT-------------------------SHEKTKKIFVGGLPEDATKEDIQEAIESLLEEKVDKVDLIMKKEDETKHRGFAFVELNNEDQADELCCVKKIHVKGKMVEAKKATPRD 190          

HSP 2 Score: 60.8474 bits (146), Expect = 1.588e-10
Identity = 32/89 (35.96%), Postives = 49/89 (55.06%), Query Frame = 2
Query:  650 NNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHF----HDLNNKTVEAKKALPKE 904
            NN    K+F+GG++  TT + L EYF  +G L    ++ D AT + RGFG+VTF +      V         H ++ K VE K+A+P++
Sbjct:    9 NNDPRAKLFVGGLNRETTNETLREYFEAYGELTDVVVICDSATKKSRGFGYVTFADYKVTRNVLKDKVENGAHRIDGKEVEVKRAIPRD 97          
BLAST of Musashi vs. Ensembl Medaka
Match: msi1 (musashi RNA binding protein 1 [Source:NCBI gene;Acc:101173434])

HSP 1 Score: 228.794 bits (582), Expect = 1.762e-67
Identity = 111/213 (52.11%), Postives = 148/213 (69.48%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLI 949
            +DP KMFIGGLS  T+ E +++YF KYG++KE M+MRDPVTKRSRGFGFVTF++ + V KVL ++ H LDSK IDPKVA PR+ +                     KL        +TKK+F+GG+S NTT +++++YF QFG ++   LM DK T+RHRGFGFVTFENED  EKVC+IHFH++NNK VE KKA PKE++   G  + R++++
Sbjct:   17 HDPCKMFIGGLSWQTTQEGLKEYFCKYGEVKECMVMRDPVTKRSRGFGFVTFVDQAGVDKVLAQSRHELDSKTIDPKVAFPRRAQP--------------------KLV------TRTKKIFVGGLSVNTTIEDVKQYFDQFGKVDDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMSPTGSARGRSRVM 203          
BLAST of Musashi vs. Ensembl Medaka
Match: msi2a (musashi RNA binding protein 2 [Source:NCBI gene;Acc:101163923])

HSP 1 Score: 226.868 bits (577), Expect = 2.403e-67
Identity = 107/200 (53.50%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG ++   LM DK T+RHRGFGF+TFE+ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFTDAASVDKVLAQQHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFITFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Medaka
Match: msi2a (musashi RNA binding protein 2 [Source:NCBI gene;Acc:101163923])

HSP 1 Score: 226.868 bits (577), Expect = 3.901e-67
Identity = 107/200 (53.50%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG ++   LM DK T+RHRGFGF+TFE+ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFTDAASVDKVLAQQHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFITFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Medaka
Match: msi2a (musashi RNA binding protein 2 [Source:NCBI gene;Acc:101163923])

HSP 1 Score: 227.639 bits (579), Expect = 1.309e-66
Identity = 107/200 (53.50%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG ++   LM DK T+RHRGFGF+TFE+ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFTDAASVDKVLAQQHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFITFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Ensembl Medaka
Match: msi2a (musashi RNA binding protein 2 [Source:NCBI gene;Acc:101163923])

HSP 1 Score: 227.639 bits (579), Expect = 1.491e-66
Identity = 107/200 (53.50%), Postives = 140/200 (70.00%), Query Frame = 2
Query:  311 NDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEII 910
            +DPGKMFIGGLS  TSP+++RDYF K+G+I+E M+MRDP TKRSRGFGFVTF +++SV KVL +  H LDSK IDPKVA PR+ +                               +TKK+F+GG+S NT  +++++YF QFG ++   LM DK T+RHRGFGF+TFE+ED  EKVC+IHFH++NNK VE KKA PKE++
Sbjct:   18 HDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFTDAASVDKVLAQQHHELDSKTIDPKVAFPRRAQPKMVT--------------------------RTKKIFVGGLSANTVVEDVKQYFEQFGKVDDAMLMFDKTTNRHRGFGFITFESEDIVEKVCEIHFHEINNKMVECKKAQPKEVM 191          
BLAST of Musashi vs. Planmine SMEST
Match: SMESG000077233.1 (SMESG000077233.1)

HSP 1 Score: 794.653 bits (2051), Expect = 0.000e+0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 2
Query:  239 MTLEIACHDXXXXXXXXXXXXPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLIXXXXXXXXXXXXXXXXXXXRQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSXXXXXXXXXXXXXXLDIYSTSDPNHITASLIQQQTSQQPHNATXXXXXXXXXXXXXXXXGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNNXXXXXXXXXXXXXXXXXXXQPGSCNGYH 1657
            MTLEIACHDESNSEYETENSSPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLISNQLQNNYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSTTNNRQCNQNNNCTLDIYSTSDPNHITASLIQQQTSQQPHNATAMIIASNNNSQANILNGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNNLLPHTAGHLAAAALSAPMLQPGSCNGYH
Sbjct:    1 MTLEIACHDESNSEYETENSSPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLISNQLQNNYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSTTNNRQCNQNNNCTLDIYSTSDPNHITASLIQQQTSQQPHNATAMIIASNNNSQANILNGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNNLLPHTAGHLAAAALSAPMLQPGSCNGYH 473          
BLAST of Musashi vs. Planmine SMEST
Match: SMESG000077233.1 (SMESG000077233.1)

HSP 1 Score: 781.941 bits (2018), Expect = 0.000e+0
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 2
Query:  239 MTLEIACHDXXXXXXXXXXXXPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLIXXXXXXXXXXXXXXXXXXXRQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSXXXXXXXXXXXXXXLDIYSTSDPNHITASLIQQQTSQQPHNATXXXXXXXXXXXXXXXXGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNN 1573
            MTLEIACHDESNSEYETENSSPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLISNQLQNNYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSTTNNRQCNQNNNCTLDIYSTSDPNHITASLIQQQTSQQPHNATAMIIASNNNSQANILNGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNN
Sbjct:    1 MTLEIACHDESNSEYETENSSPICNDPGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLISNQLQNNYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNSPLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLFMSTTNNRQCNQNNNCTLDIYSTSDPNHITASLIQQQTSQQPHNATAMIIASNNNSQANILNGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNN 445          
BLAST of Musashi vs. Planmine SMEST
Match: SMESG000071202.1 (SMESG000071202.1)

HSP 1 Score: 234.958 bits (598), Expect = 1.807e-68
Identity = 115/219 (52.51%), Postives = 157/219 (71.69%), Query Frame = 2
Query:  317 PGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIG-NNGVLKQRNQLIRTSLPLI 970
            P K+F+GGL+PTT+ E ++ YF ++G+IKEFM+MRD V KRSRGFGFVTF + S V KVL +  H LDSKKIDPK+AVP+ I      C               K+S      A+TKK+FIGGI+T++T +E+ +YF+++G +E CELMMDKAT+RHRGFGFVTF +ED AE++C IH+H++NNK VEAKKALPKE++  +N   K  N+ ++  L ++
Sbjct:   36 PCKLFVGGLNPTTTSEALQSYFSQFGEIKEFMVMRDIVHKRSRGFGFVTFTDYSGVIKVLKQENHILDSKKIDPKLAVPKSI------CQPE------------KIS------ARTKKIFIGGIATSSTVEEIRQYFNRYGEVENCELMMDKATNRHRGFGFVTFLDEDVAEQICQIHYHNINNKMVEAKKALPKELLSTSNATPKSENKSLQVPLAIL 230          
BLAST of Musashi vs. Planmine SMEST
Match: SMESG000071202.1 (SMESG000071202.1)

HSP 1 Score: 234.958 bits (598), Expect = 1.901e-68
Identity = 115/219 (52.51%), Postives = 157/219 (71.69%), Query Frame = 2
Query:  317 PGKMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKEIIG-NNGVLKQRNQLIRTSLPLI 970
            P K+F+GGL+PTT+ E ++ YF ++G+IKEFM+MRD V KRSRGFGFVTF + S V KVL +  H LDSKKIDPK+AVP+ I      C               K+S      A+TKK+FIGGI+T++T +E+ +YF+++G +E CELMMDKAT+RHRGFGFVTF +ED AE++C IH+H++NNK VEAKKALPKE++  +N   K  N+ ++  L ++
Sbjct:   44 PCKLFVGGLNPTTTSEALQSYFSQFGEIKEFMVMRDIVHKRSRGFGFVTFTDYSGVIKVLKQENHILDSKKIDPKLAVPKSI------CQPE------------KIS------ARTKKIFIGGIATSSTVEEIRQYFNRYGEVENCELMMDKATNRHRGFGFVTFLDEDVAEQICQIHYHNINNKMVEAKKALPKELLSTSNATPKSENKSLQVPLAIL 238          
BLAST of Musashi vs. Planmine SMEST
Match: SMESG000045507.1 (SMESG000045507.1)

HSP 1 Score: 182.57 bits (462), Expect = 2.623e-53
Identity = 85/158 (53.80%), Postives = 118/158 (74.68%), Query Frame = 2
Query:  323 KMFIGGLSPTTSPETMRDYFRKYGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDPKVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGF 796
            K+FIGGLSP T+ ET+R+YF+ YGDIK+ MI++DPVTKRSRGFGFVTF++ S+++K+L  +PHFLDSKKIDPK+A+P+K E                      L D ++N  KT+KVFIGG+S +T  ++LE +F ++G +E+CELMMDK+T+RHRG+
Sbjct:   18 KLFIGGLSPHTTLETLRNYFQNYGDIKDVMILKDPVTKRSRGFGFVTFIDYSTIEKILLNSPHFLDSKKIDPKIAIPKKPE----------------------LIDKAINTEKTRKVFIGGLSNSTVHKDLEAFFGKYGKVESCELMMDKSTNRHRGY 153          

HSP 2 Score: 64.3142 bits (155), Expect = 9.670e-12
Identity = 33/80 (41.25%), Postives = 51/80 (63.75%), Query Frame = 2
Query:  665 KKVFIGGISTNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKVCDIHFHDLNNKTVEAKKALPKE 904
            KK+FIGG+S +TT + L  YF  +G ++   ++ D  T R RGFGFVTF +  + EK+     H L++K ++ K A+PK+
Sbjct:   17 KKLFIGGLSPHTTLETLRNYFQNYGDIKDVMILKDPVTKRSRGFGFVTFIDYSTIEKILLNSPHFLDSKKIDPKIAIPKK 96          
The following BLAST results are available for this feature:
BLAST of Musashi vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MSI24.779e-6855.00musashi RNA binding protein 2 [Source:HGNC Symbol;... [more]
MSI21.277e-6554.59musashi RNA binding protein 2 [Source:HGNC Symbol;... [more]
MSI13.812e-6549.30musashi RNA binding protein 1 [Source:HGNC Symbol;... [more]
MSI28.386e-6554.36musashi RNA binding protein 2 [Source:HGNC Symbol;... [more]
DAZAP15.655e-4541.03DAZ associated protein 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of Musashi vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
msi-13.216e-6452.26MuSashI (Fly neural) family; Neural RNA-binding pr... [more]
hrp-14.487e-3836.60Heterogeneous nuclear ribonucleoprotein A1 [Sourc... [more]
hrp-14.487e-3836.60Heterogeneous nuclear ribonucleoprotein A1 [Sourc... [more]
hrp-14.588e-3836.60Heterogeneous nuclear ribonucleoprotein A1 [Sourc... [more]
hrp-14.588e-3836.60Heterogeneous nuclear ribonucleoprotein A1 [Sourc... [more]
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BLAST of Musashi vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rbp61.927e-6651.66gene:FBgn0260943 transcript:FBtr0075285[more]
Rbp62.769e-6651.66gene:FBgn0260943 transcript:FBtr0333861[more]
Rbp66.750e-6651.66gene:FBgn0260943 transcript:FBtr0113427[more]
msi1.290e-5545.54gene:FBgn0011666 transcript:FBtr0333665[more]
msi2.105e-5545.54gene:FBgn0011666 transcript:FBtr0084882[more]
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BLAST of Musashi vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
msi2b1.201e-6855.00musashi RNA-binding protein 2b [Source:ZFIN;Acc:ZD... [more]
msi2b7.661e-6855.00musashi RNA-binding protein 2b [Source:ZFIN;Acc:ZD... [more]
msi2b1.099e-6654.59musashi RNA-binding protein 2b [Source:ZFIN;Acc:ZD... [more]
msi2a2.244e-6652.09musashi RNA-binding protein 2a [Source:ZFIN;Acc:ZD... [more]
msi2a2.244e-6652.09musashi RNA-binding protein 2a [Source:ZFIN;Acc:ZD... [more]
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BLAST of Musashi vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kcnj45.023e-6754.50potassium inwardly rectifying channel subfamily J ... [more]
kcnj41.117e-6654.50potassium inwardly rectifying channel subfamily J ... [more]
msi11.147e-6650.00musashi RNA binding protein 1 [Source:NCBI gene;Ac... [more]
msi11.189e-6650.00musashi RNA binding protein 1 [Source:NCBI gene;Ac... [more]
kcnj41.679e-6654.50potassium inwardly rectifying channel subfamily J ... [more]
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BLAST of Musashi vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Msi23.562e-6855.00musashi RNA-binding protein 2 [Source:MGI Symbol;A... [more]
Msi23.980e-6855.00musashi RNA-binding protein 2 [Source:MGI Symbol;A... [more]
Msi15.394e-6549.30musashi RNA-binding protein 1 [Source:MGI Symbol;A... [more]
Msi11.178e-5847.74musashi RNA-binding protein 1 [Source:MGI Symbol;A... [more]
Msi13.818e-5847.96musashi RNA-binding protein 1 [Source:MGI Symbol;A... [more]
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BLAST of Musashi vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q96DH6|MSI2H_HUMAN2.295e-6755.00RNA-binding protein Musashi homolog 2 OS=Homo sapi... [more]
sp|Q920Q6|MSI2H_MOUSE2.784e-6755.00RNA-binding protein Musashi homolog 2 OS=Mus muscu... [more]
sp|Q9VVE5|MSIR6_DROME1.932e-6551.66RNA-binding protein Musashi homolog Rbp6 OS=Drosop... [more]
sp|O43347|MSI1H_HUMAN1.831e-6449.30RNA-binding protein Musashi homolog 1 OS=Homo sapi... [more]
sp|Q61474|MSI1H_MOUSE3.773e-6449.30RNA-binding protein Musashi homolog 1 OS=Mus muscu... [more]
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BLAST of Musashi vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
B1Q3I90.000e+092.60Musashi OS=Dugesia japonica OX=6161 GN=DjmlgC PE=2... [more]
A0A0A7M1Y47.355e-10299.36Musashi-like gene C (Fragment) OS=Schmidtea polych... [more]
A0A3Q0KQN17.766e-8561.93Rna-binding protein musashi-related OS=Schistosoma... [more]
G4V9P31.317e-8462.79Rna-binding protein musashi-related OS=Schistosoma... [more]
A0A5K4ET691.526e-8461.93Rna-binding protein musashi-related OS=Schistosoma... [more]
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BLAST of Musashi vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
msi2a1.480e-6855.00RNA-binding protein Musashi homolog 2-like [Source... [more]
msi2b2.080e-6855.00RNA-binding protein Musashi homolog 2 [Source:NCBI... [more]
msi2b2.195e-6855.00RNA-binding protein Musashi homolog 2 [Source:NCBI... [more]
msi2a3.764e-6855.00RNA-binding protein Musashi homolog 2-like [Source... [more]
msi2a5.550e-6855.00RNA-binding protein Musashi homolog 2-like [Source... [more]
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BLAST of Musashi vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HNRNPA05.289e-4038.14heterogeneous nuclear ribonucleoprotein A0 [Source... [more]
HNRNPA04.134e-3937.11heterogeneous nuclear ribonucleoprotein A0 [Source... [more]
ENSPMAT00000004893.12.978e-3735.00pep scaffold:Pmarinus_7.0:GL479484:4:2056:-1 gene:... [more]
HNRNPA2B12.504e-3635.75heterogeneous nuclear ribonucleoprotein A2/B1 [Sou... [more]
RBM393.524e-1424.86RNA binding motif protein 39 [Source:HGNC Symbol;A... [more]
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BLAST of Musashi vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
HRP12.475e-4240.51Subunit of cleavage factor I; cleavage factor I is... [more]
NSR11.958e-1125.99Nucleolar protein that binds nuclear localization ... [more]
PAB11.338e-1025.98Poly(A) binding protein; part of the 3'-end RNA-pr... [more]
PUB19.037e-923.20Poly (A)+ RNA-binding protein; abundant mRNP-compo... [more]
SGN14.719e-725.38Cytoplasmic RNA-binding protein; contains an RNA r... [more]
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BLAST of Musashi vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO398791.040e-5951.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO333891.734e-4342.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO384174.993e-3635.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO493495.601e-3437.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO365221.043e-3233.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Musashi vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
msi11.762e-6752.11musashi RNA binding protein 1 [Source:NCBI gene;Ac... [more]
msi2a2.403e-6753.50musashi RNA binding protein 2 [Source:NCBI gene;Ac... [more]
msi2a3.901e-6753.50musashi RNA binding protein 2 [Source:NCBI gene;Ac... [more]
msi2a1.309e-6653.50musashi RNA binding protein 2 [Source:NCBI gene;Ac... [more]
msi2a1.491e-6653.50musashi RNA binding protein 2 [Source:NCBI gene;Ac... [more]
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BLAST of Musashi vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000077233.10.000e+0100.00SMESG000077233.1[more]
SMESG000077233.10.000e+0100.00SMESG000077233.1[more]
SMESG000071202.11.807e-6852.51SMESG000071202.1[more]
SMESG000071202.11.901e-6852.51SMESG000071202.1[more]
SMESG000045507.12.623e-5353.80SMESG000045507.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018588 ID=SMED30018588|Name=Musashi|organism=Schmidtea mediterranea sexual|type=transcript|length=2495bp
TTAATTTACATACTACTGACTGATTAAATTGCTTAAGAAATTTTTTTTGG
GTATTCGGTTTGCTAAGTTTGCAATCCAGATTTCTTTTATATTTTTCCAA
TTCAAAATCGCAAGTTTTGTTTTTCGTGAATTGCATTTTTTTTGAGATCT
CGCAACAATTACAAACCCAATTGGAATATTTTTTTAAAATTTGTGTTAAT
TTAAACAAAAATTATTTAACAAAAATAATTTCTAAATCATGACTTTGGAA
ATCGCGTGCCATGATGAATCAAATAGTGAATACGAAACAGAAAACAGTAG
CCCAATTTGCAATGATCCAGGTAAAATGTTCATTGGAGGATTGAGCCCGA
CAACTTCGCCAGAAACCATGAGAGACTACTTCCGAAAATATGGGGACATA
AAAGAATTTATGATAATGCGAGACCCCGTAACGAAACGGTCCAGAGGATT
CGGATTTGTTACGTTTTTGGAGAGCTCAAGTGTCAAAAAAGTTCTCAACA
AAACTCCACATTTTTTAGATTCAAAAAAGATTGATCCAAAAGTTGCTGTT
CCGAGAAAAATAGAATCGTCTACCAAAAATTGTGACACCTCCATTGACTG
TCTTGGGATAAATGGTTATTCTTCTGGAAAACTTAGCGACTCGAGTCTGA
ATAATGCGAAAACTAAGAAAGTTTTCATTGGTGGAATATCCACAAACACA
ACAAAACAAGAATTAGAGGAATATTTTTCTCAATTTGGAATCTTAGAAAC
TTGTGAATTGATGATGGATAAAGCTACGAGTCGTCACAGAGGATTTGGCT
TTGTGACATTTGAAAACGAGGACTCAGCGGAAAAAGTCTGCGATATTCAT
TTCCATGATCTAAACAATAAAACAGTTGAAGCAAAAAAAGCTTTACCAAA
AGAAATTATCGGAAATAACGGAGTTTTGAAACAACGCAATCAGTTGATCA
GGACCTCATTACCTCTGATATCAAATCAATTGCAAAACAATTATGCCTTG
AATGCCCTCTCGGCTATAGCGGCTGCTAGGCAGTGTAATACATTCCTAAT
GCCAAGCTATTATATAACTTCAGGATTAATACCGAGTAATTTCATGCTGC
AAGCTCCTGATTCTCTCCCTGCTCCTGTTACGAATTCTCCTTTAATAAGC
AATCTACAGTCAGCTCGATCTATTCCTTTGTATTCGACAAATCATCCCTC
CGAACAGCTTATGTCATTAAACGCTGGCCACCTCATTAATGGCAATGTAG
CAGGACCTGTCCTCTACAGTCCATCAACCCCATTATTTATGTCCACTACC
AACAATAGACAATGTAATCAAAACAACAACTGTACATTAGACATTTATTC
CACATCTGATCCAAATCATATTACAGCATCTTTAATTCAACAACAGACTT
CCCAACAACCTCACAACGCAACGGCTATGATTATCGCTTCAAACAACAAT
TCCCAAGCAAATATTCTCAATGGGGTATCGGTGAAAAATACCATATTCAG
TAATGGCTGCGATTCAGTAACTCAGATAGCTCAGCATATACCAACGGCTC
ATTATCTTGGAGCTACTAATAATTTGTTACCTCACACTGCTGGGCATTTA
GCAGCTGCTGCTTTATCCGCTCCAATGCTTCAACCAGGATCTTGTAATGG
TTACCATTGATCATTTGACAATGATCTGACAAAAAATAGAAGAAAAAAAA
TTTATTCTTATTCTTGTTTGTTGATATGCTTCTGAATACATAAAACCTTA
ATCGTGCTTCTAGCCATATAAAAATTTTTCTTCAAATTTCAACTCGAAAG
TTCAAAGTTTTACAGACTGCGTATGTGGACATATCAGAAGAATAAAATGA
AAAATAAAAAATGAGAAACGTTTCCCTAAATGATATTTAATTAAGAAAAA
AATTAAAACTGATTTTTGCGAAGCAGACTAAATATTTTTCATTTTAATGC
GTGTTTCTTTATTATTTTATTAATTATTTATTCGAAAAAAAAACTTTTGA
AAAAATTTAATTTTATGTAATCTATTTTTTTATTGTTTATTCTGTTGAAT
TTCGTGTCATGTTGATGAGAAAAAATATATATAAATATATAAATAAGTAA
ATAGAAACTATGAAACGTTATATAAATTTGTAAATACGCCTGTTTGTTGG
AAATTTCACCAGGTTATAAATGTAAATCTTCTTTTTCAAAAGAAAACACA
AGAGGTAAAAAAAACATTTCTTTTCTTTCTTGCGATCGAATCTTTGTCAA
AGAGAAAACATCTTGTGATATTATTGAAAAAAAAGACGATTATTTTATAT
CTATTCAACGTGAATACCGATCATTTTAATATGAGATTGTGATTTTTTAT
TTATCATTTATATTTAATCATGCTTGTTTTAATGTTTCGATTTTTGGTGA
AATTTTAAATTAGTGTTTGTAATGGTATTCTAATTTGAAAAATACTAAAA
ATCGAAAAGATGTTTTCTGAATTTTTTGATGAGAAAATTTATGCA
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protein sequence of SMED30018588-orf-1

>SMED30018588-orf-1 ID=SMED30018588-orf-1|Name=SMED30018588-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=474bp
MTLEIACHDESNSEYETENSSPICNDPGKMFIGGLSPTTSPETMRDYFRK
YGDIKEFMIMRDPVTKRSRGFGFVTFLESSSVKKVLNKTPHFLDSKKIDP
KVAVPRKIESSTKNCDTSIDCLGINGYSSGKLSDSSLNNAKTKKVFIGGI
STNTTKQELEEYFSQFGILETCELMMDKATSRHRGFGFVTFENEDSAEKV
CDIHFHDLNNKTVEAKKALPKEIIGNNGVLKQRNQLIRTSLPLISNQLQN
NYALNALSAIAAARQCNTFLMPSYYITSGLIPSNFMLQAPDSLPAPVTNS
PLISNLQSARSIPLYSTNHPSEQLMSLNAGHLINGNVAGPVLYSPSTPLF
MSTTNNRQCNQNNNCTLDIYSTSDPNHITASLIQQQTSQQPHNATAMIIA
SNNNSQANILNGVSVKNTIFSNGCDSVTQIAQHIPTAHYLGATNNLLPHT
AGHLAAAALSAPMLQPGSCNGYH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003729mRNA binding
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
GO:0003723RNA binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000025nervous system
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0003503ventral epidermis progenitor cell
Vocabulary: INTERPRO
TermDefinition
IPR037366BOULE/DAZ
IPR034126MSI_RRM2
IPR035979RBD_domain_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 144..216
e-value: 1.3E-23
score: 94.5
coord: 29..101
e-value: 8.8E-21
score: 85.0
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 145..213
e-value: 1.0E-17
score: 63.7
coord: 31..89
e-value: 2.1E-15
score: 56.3
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 143..220
score: 17.131
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 28..113
score: 15.784
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 142..218
e-value: 5.8E-24
score: 86.2
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 26..108
e-value: 3.1E-21
score: 77.5
IPR037366BOULE/DAZ familyPANTHERPTHR11176BOULE-RELATEDcoord: 25..113
IPR037366BOULE/DAZ familyPANTHERPTHR11176BOULE-RELATEDcoord: 137..233
NoneNo IPR availablePANTHERPTHR11176:SF13GENE 17190-RELATEDcoord: 25..113
NoneNo IPR availablePANTHERPTHR11176:SF13GENE 17190-RELATEDcoord: 137..233
IPR034126RNA-binding protein Musashi homologue, RNA recognition motif 2CDDcd12323RRM2_MSIcoord: 144..217
e-value: 4.1967E-43
score: 144.885
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 28..111
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 137..222