SMED30018579
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30018579 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16
Alignments
SMED30018579 aligns in the following genomic locations:
Homology
BLAST of SMED30018579 vs. Ensembl Human
Match: CDT1 (chromatin licensing and DNA replication factor 1 [Source:HGNC Symbol;Acc:HGNC:24576]) HSP 1 Score: 60.077 bits (144), Expect = 1.340e-8 Identity = 78/358 (21.79%), Postives = 152/358 (42.46%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLI--YDNRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQH-VPS-KNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKP------TEFDSSKVSPLKTGD-----------------NHRLGSLSQSLIDKVRMNKANRSV--ITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV 2367 LP +VL E F+ ++ ++ NR + T ++ V+ + R+F EC +G+I VYP +Y R + VP+ K+G R S ++ I P L +S L +R Q F + V+ V ++ F+ + S V ++ R W+ FN++ +V +EP +PQ ++L A+ + + P ++ +P G L +SQ L++++R +A + + +T P +R +LPE+A ++ + F + + KP + + + C + + C +M G ++L +L +++P W+ Sbjct: 186 LPYKYQVLAEMFRSMDTIVGMLHNRSETPTFAKVQRGVQDMMR-RRFEECNVGQIKTVYPASYRFRQERSVPTFKDGTRRSDYQLTIEPLLEQEADGAAPQLTASRLL--------QRRQIFSQKLVEHVKEHHKAFLASLSPAMVVPEDQLTR---WHPRFNVD-EVPDIEPAALPQPPATEKLTTAQEVLARARNLISPRMEKALSQLALRSAAPSSPGSPRPALPATPPATPPAASPSALKGVSQDLLERIRAKEAQKQLAQMTRCPEQEQRLQRLERLPELARVLRSVF-VSERKP---ALSMEVACARM----------------VGSCCTIMSPGEMEKHLLLLSELLPDWL 510
BLAST of SMED30018579 vs. Ensembl Zebrafish
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:ZFIN;Acc:ZDB-GENE-070111-1]) HSP 1 Score: 81.6481 bits (200), Expect = 1.780e-15 Identity = 105/399 (26.32%), Postives = 180/399 (45.11%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLIYD--NRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQH--VPSKNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFI--LNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENAR--------LTFSVMKKN--------------NKPTEFDSSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV------KDLNWAVPRLTIGTPDLSLDEVLLRLNANVAKQQ 2469 LP K+L E F+ LE ++ NR + T T ++ V+ + R F E LG+I AVYP AY+ R + + + S+ ++ + +RP L +S L +R F +N V+IV ++ F+ LN I+ + + W+ F+++ +V V+P +PQ Q ++L +A+ L M+K +P S V+P +T L +SQSL++++R +A + +V+T P R M S+LPE+A I+ N F + + KP + ++ L C NR+ I Y R D ++L +L ++ P W+ KDL + + T DLS+ VL +LN +++ Sbjct: 321 LPYQYKLLAEMFRSLETIVAMLFNRSETVTFTKVKQGVQDMMRKR-FEESHLGQIKAVYPSAYTFRQEKNIISFSATAKRSSYQLTVEPVIDEEFKGVRPVLSASRLL--------ERRHIFHQNLVEIVKGHHKTFLASLNPPIVVPDE-----KLTRWHPRFSVD-EVPNVKPSDLPQPPQTEKLTSAQEVLDKARALMTPKMEKALANMALKTAEKACVKEPETTAKSAVTPTETPGA--LKGVSQSLLERIRAKEAQKLHAVMTRNPQQEERLLMMSRLPELARILRNVF-VAEKKP---ALIMELAC--------NRM---IASY---RSPLTSD--EMEKHLRLLAELTPAWLTIHPIRKDLYLKLNKTT----DLSI--VLDKLNQKTKEEE 676
BLAST of SMED30018579 vs. Ensembl Zebrafish
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:ZFIN;Acc:ZDB-GENE-070111-1]) HSP 1 Score: 55.0694 bits (131), Expect = 2.554e-7 Identity = 52/191 (27.23%), Postives = 92/191 (48.17%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLIYD--NRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQH--VPSKNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFI--LNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENAR 1935 LP K+L E F+ LE ++ NR + T T ++ V+ + R F E LG+I AVYP AY+ R + + + S+ ++ + +RP L +S L +R F +N V+IV ++ F+ LN I+ + + W+ F+++ +V V+P +PQ Q ++L +A+ Sbjct: 271 LPYQYKLLAEMFRSLETIVAMLFNRSETVTFTKVKQGVQDMMRKR-FEESHLGQIKAVYPSAYTFRQEKNIISFSATAKRSSYQLTVEPVIDEEFKGVRPVLSASRLL--------ERRHIFHQNLVEIVKGHHKTFLASLNPPIVVPDE-----KLTRWHPRFSVD-EVPNVKPSDLPQPPQTEKLTSAQ 446
BLAST of SMED30018579 vs. Ensembl Xenopus
Match: CDT1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:100379812]) HSP 1 Score: 71.2478 bits (173), Expect = 4.426e-12 Identity = 85/359 (23.68%), Postives = 161/359 (44.85%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLI--YDNRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQ-HVPS-KNGLRSSTNKIDIAI----------RPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFI--LNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDN---------------------HRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV 2367 LP KVL E F+ ++ ++ NR + T + ++ V+ + +QF + +G+I VYP AY R + ++P+ K+G++ + ++ I RP+L +S+ L +R Q F + IV ++ F+ LN ++ ++ + W+ FN++ +V V P +P Q ++L A+ S + P + LKT +N + L +SQSL++++R +A + +++T P R M S+LPE+A I+ N F + + KP + + + C NR +I M G ++L +L +++P W+ Sbjct: 258 LPYKYKVLAEMFRSMDTIVGMLFNRSETITFSKVKQGVQDMM-RKQFEQRNVGQIKTVYPTAYKYRQEKNIPTFKDGVKKTDYQLTIDPLIDEGDKLNGRPHLSASSLL--------QRRQLFHRSLTSIVKQHHKVFLASLNPPMVVPDD-----KLTRWHPRFNVD-EVPDVMPSELPLPPQVEKLTTAQEVLSKARGMITPKMEKALANLALKTAENAGEPKNVSSEETKSAATASTSNALKGVSQSLLERIRAKEAQKLQAMMTRRPQQEERLLMMSRLPELARILRNVF-VAEKKP---ALTLEVTC--------NR--------VISSYRSSMSPGEMEKHLALLSEILPDWL 581
BLAST of SMED30018579 vs. Ensembl Xenopus
Match: CDT1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:100379812]) HSP 1 Score: 71.2478 bits (173), Expect = 4.711e-12 Identity = 85/359 (23.68%), Postives = 161/359 (44.85%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLI--YDNRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQ-HVPS-KNGLRSSTNKIDIAI----------RPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFI--LNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDN---------------------HRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV 2367 LP KVL E F+ ++ ++ NR + T + ++ V+ + +QF + +G+I VYP AY R + ++P+ K+G++ + ++ I RP+L +S+ L +R Q F + IV ++ F+ LN ++ ++ + W+ FN++ +V V P +P Q ++L A+ S + P + LKT +N + L +SQSL++++R +A + +++T P R M S+LPE+A I+ N F + + KP + + + C NR +I M G ++L +L +++P W+ Sbjct: 211 LPYKYKVLAEMFRSMDTIVGMLFNRSETITFSKVKQGVQDMM-RKQFEQRNVGQIKTVYPTAYKYRQEKNIPTFKDGVKKTDYQLTIDPLIDEGDKLNGRPHLSASSLL--------QRRQLFHRSLTSIVKQHHKVFLASLNPPMVVPDD-----KLTRWHPRFNVD-EVPDVMPSELPLPPQVEKLTTAQEVLSKARGMITPKMEKALANLALKTAENAGEPKNVSSEETKSAATASTSNALKGVSQSLLERIRAKEAQKLQAMMTRRPQQEERLLMMSRLPELARILRNVF-VAEKKP---ALTLEVTC--------NR--------VISSYRSSMSPGEMEKHLALLSEILPDWL 534
BLAST of SMED30018579 vs. UniProt/SwissProt
Match: sp|Q9I9A7|CDT1_XENLA (DNA replication factor Cdt1 OS=Xenopus laevis OX=8355 GN=cdt1 PE=1 SV=2) HSP 1 Score: 76.6406 bits (187), Expect = 4.867e-13 Identity = 85/359 (23.68%), Postives = 158/359 (44.01%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLI--YDNRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQ-HVPS-KNGLRSSTNKIDI----------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFI--LNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDN---------------------HRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV 2367 LP KVL E F+ ++ ++ NR + T + ++ V+ + +QF + +G+I VYP AY R + ++P+ K+G++ + ++ I + RP+L +S L +R Q F + IV +R F+ LN ++ ++ + W+ FN++ +V V P +P Q + L A+ S + P + LKT +N L +SQSL++++R +A + +++T P R M S+LPE+A I+ N F + + KP + + + C +I C M G ++L +L +++P W+ Sbjct: 258 LPYKYKVLAEMFRSMDTIVGMLFNRSETITFSKVKQGVQDMM-RKQFEQRNVGQIKTVYPNAYKYRQEKNIPTFKDGVKKTDYQLTIEPLVAEGDMLSGRPHLSASRLL--------ERKQLFHRSLTSIVKQHHRVFLTSLNPPMLVPDD-----KLTRWHPRFNVD-EVLDVTPAELPLPPQVERLTTAQEVLSKARGLITPKMEKALANLALKTAENAGETKNVSTEETKSTATTSTSTALKGVSQSLLERIRAKEAQKLQAIMTRRPQQEERLLMMSRLPELARILRNVF-VAEKKP---ALTLEVTCSR----------------VIASCRSSMSPGEMEKHLALLSEILPDWL 581
BLAST of SMED30018579 vs. UniProt/SwissProt
Match: sp|Q9H211|CDT1_HUMAN (DNA replication factor Cdt1 OS=Homo sapiens OX=9606 GN=CDT1 PE=1 SV=3) HSP 1 Score: 60.077 bits (144), Expect = 6.437e-8 Identity = 78/358 (21.79%), Postives = 152/358 (42.46%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLI--YDNRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQH-VPS-KNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKP------TEFDSSKVSPLKTGD-----------------NHRLGSLSQSLIDKVRMNKANRSV--ITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV 2367 LP +VL E F+ ++ ++ NR + T ++ V+ + R+F EC +G+I VYP +Y R + VP+ K+G R S ++ I P L +S L +R Q F + V+ V ++ F+ + S V ++ R W+ FN++ +V +EP +PQ ++L A+ + + P ++ +P G L +SQ L++++R +A + + +T P +R +LPE+A ++ + F + + KP + + + C + + C +M G ++L +L +++P W+ Sbjct: 186 LPYKYQVLAEMFRSMDTIVGMLHNRSETPTFAKVQRGVQDMMR-RRFEECNVGQIKTVYPASYRFRQERSVPTFKDGTRRSDYQLTIEPLLEQEADGAAPQLTASRLL--------QRRQIFSQKLVEHVKEHHKAFLASLSPAMVVPEDQLTR---WHPRFNVD-EVPDIEPAALPQPPATEKLTTAQEVLARARNLISPRMEKALSQLALRSAAPSSPGSPRPALPATPPATPPAASPSALKGVSQDLLERIRAKEAQKQLAQMTRCPEQEQRLQRLERLPELARVLRSVF-VSERKP---ALSMEVACARM----------------VGSCCTIMSPGEMEKHLLLLSELLPDWL 510
BLAST of SMED30018579 vs. TrEMBL
Match: A0A0X3PKA8 (DNA replication factor Cdt1 (Fragment) OS=Schistocephalus solidus OX=70667 GN=CDT1 PE=4 SV=1) HSP 1 Score: 82.8037 bits (203), Expect = 1.513e-12 Identity = 49/173 (28.32%), Postives = 90/173 (52.02%), Query Frame = 1 Query: 181 NTILIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEK--MKIIPNF--NFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLETSVQPI 687 +T+ +P H +L+ F + D+VV+ L + DKV ++E+ ++F I Q ++P+SYS+ +D KVT+ + +IPN + + P K VF ++L+ R H + L+ +V+ H EFL+ ++S+ ++ + K+WH F L+T+V I Sbjct: 149 STLPLPSHMHTLLEFFRACDLVVSVLHNRSEVCSFDKVKLAVQELVRSDFTEAIVSQFATVYPDSYSFRYDKQLDKVTKLPTSSYVLILIPNLRSDGTQMARDSPSKGHLVFTGNRLIQRRHHFQKSLIARVRRAHREFLVNKLSISESDLPPDSPIKRWHPAFPLDTAVPTI 321 HSP 2 Score: 70.4774 bits (171), Expect = 9.653e-9 Identity = 86/384 (22.40%), Postives = 157/384 (40.89%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELE---KLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSN-----------FLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKE------------------LENARLT--FSVMKKNNKPTEFDSSKVSP------LKTGDNHRLGSLSQSLIDKVR-MNKANR-SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVL 2436 LP+H+ L E F+ + ++++ C+ ++ AV+ L FTE + + VYP++YS R K +++ + I I PNL S L F + +R F ++ + V ++REF++NK I ++ + W+ F ++ V T+ P+P K E A + FS ++ K T + S SP L + L +S+SL+ +VR KA + S++T +P + ++ S LP T + E + KP+ + + + +S + S L+ +D L ++P W++ + WA P L + D L +V+ Sbjct: 153 LPSHMHTLLEFFRACDLVVSVLHNRSEVCSFDKVKLAVQELVRS-DFTEAIVSQFATVYPDSYSFRYDKQLDKVTKLPTSSYVLILI-PNLRSDGTQMARDSPSKGHLVFTGNRLIQRRHHFQKSLIARVRRAHREFLVNKLSISESDLPPDSPIKRWHPAFPLDTAVPTIPSTPLPPKPDANESKITSASEAVAAFRSRALFREAEICERFSPHPQDAKKT-YKSVVPSPSQASGSLTKSNAVALKGISESLLARVREREKAMQFSLLTRSPISEKQRLALSCLPTTITQVWRELRALSGKPVPLATVATRLAQSSETGLSQEAALY--------------------RIDELISLMPDWLEKVAWAKPHLRLKDMDRPLKDVI 513
BLAST of SMED30018579 vs. TrEMBL
Match: A0A183TKL1 (DNA replication factor Cdt1 OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS17009 PE=4 SV=1) HSP 1 Score: 82.4185 bits (202), Expect = 1.742e-12 Identity = 49/173 (28.32%), Postives = 90/173 (52.02%), Query Frame = 1 Query: 181 NTILIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEK--MKIIPNF--NFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLETSVQPI 687 +T+ +P H +L+ F + D+VV+ L + DKV ++E+ ++F I Q ++P+SYS+ +D KVT+ + +IPN + + P K VF ++L+ R H + L+ +V+ H EFL+ ++S+ ++ + K+WH F L+T+V I Sbjct: 171 STLPLPSHMHTLLEFFRACDLVVSVLHNRSEVCSFDKVKLAVQELVRSDFTEAIVSQFATVYPDSYSFRYDKQLDKVTKLPTSSYVLILIPNLRSDGTQMARDSPSKGHLVFTGNRLIQRRHHFQKSLIARVRRAHREFLVNKLSISESDLPPDSPIKRWHPAFPLDTAVPTI 343 HSP 2 Score: 70.4774 bits (171), Expect = 1.021e-8 Identity = 86/384 (22.40%), Postives = 157/384 (40.89%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELE---KLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSN-----------FLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKE------------------LENARLT--FSVMKKNNKPTEFDSSKVSP------LKTGDNHRLGSLSQSLIDKVR-MNKANR-SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVL 2436 LP+H+ L E F+ + ++++ C+ ++ AV+ L FTE + + VYP++YS R K +++ + I I PNL S L F + +R F ++ + V ++REF++NK I ++ + W+ F ++ V T+ P+P K E A + FS ++ K T + S SP L + L +S+SL+ +VR KA + S++T +P + ++ S LP T + E + KP+ + + + +S + S L+ +D L ++P W++ + WA P L + D L +V+ Sbjct: 175 LPSHMHTLLEFFRACDLVVSVLHNRSEVCSFDKVKLAVQELVRS-DFTEAIVSQFATVYPDSYSFRYDKQLDKVTKLPTSSYVLILI-PNLRSDGTQMARDSPSKGHLVFTGNRLIQRRHHFQKSLIARVRRAHREFLVNKLSISESDLPPDSPIKRWHPAFPLDTAVPTIPSTPLPPKPDANESKITSASEAVAAFRSRALFREAEICERFSPHPQDAKKT-YKSVVPSPSQASGSLTKSNAVALKGISESLLARVREREKAMQFSLLTRSPISEKQRLALSCLPTTITQVWRELRALSGKPVPLATVATRLAQSSETGLSQEAALY--------------------RIDELISLMPDWLEKVAWAKPHLRLKDMDRPLKDVI 535
BLAST of SMED30018579 vs. TrEMBL
Match: A0A4Z2D6X1 (DNA replication factor Cdt1 OS=Schistosoma japonicum OX=6182 GN=EWB00_004002 PE=4 SV=1) HSP 1 Score: 82.4185 bits (202), Expect = 2.249e-12 Identity = 95/420 (22.62%), Postives = 172/420 (40.95%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELE---KLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLE-----------SSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELE-----------NARLTF---------SVMKKNNKPTEF-DSSKVSPLKT---------GDN---------------------HRLGSLSQSLIDKVRMNKANRSVIT----GAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVLLRLNANVAK 2463 LP +L+VL E F+ + ++++ + C+ L+ AV+ + R F E +G+ L +YP Y +R + K RS+ N + + + PNL S FL F + +R +F + +V+ S+REF++++ I + E W+ F +E V ++ P P+P + + E + AR F S+ K+ P SPLK DN + L +S +L++KVR + R ++T G + RR +LP I T + + + + +P+ S + V + + + SE+LD+L ++ P W++ LNW+ L + P+ S+ +V+ + VAK Sbjct: 177 LPHNLRVLLELFRSCDTVVSMLHNRKELCSFDKLQPAVQEIV-RRNFEETHVGQFLTIYPMVYFLRYEKQLDKFTHRSNGNYV-LVLSPNLRTDGTQIGHDSPSKGFLPFTGTRLIQRRNRFHSLLLNLVLKSHREFLISELGIDPSKLPEDSSLRRWHPKFPLESAVTSILPSPLPVRPSEFEQKITSAKDAVKAFQARALFREAETCHQISLSKQQLSPIPSPKKPAASPLKASNIIGSTVCADNVMGMQTNSEESTSKRIITSTPNTLKGVSLALLNKVRARENERRLLTELTYGKVSETRRAIYC-RLPLIVTQVWSILRSSNARPVPLSTVAVRVADAHPSG--------------------ISADAISEHLDVLVELAPCWIEKLNWSTVHLRLKDPERSVKDVIDLVKMKVAK 573
BLAST of SMED30018579 vs. TrEMBL
Match: E7F2R5 (Chromatin licensing and DNA replication factor 1 OS=Danio rerio OX=7955 GN=cdt1 PE=1 SV=1) HSP 1 Score: 81.6481 bits (200), Expect = 3.983e-12 Identity = 105/399 (26.32%), Postives = 180/399 (45.11%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLIYD--NRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQH--VPSKNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFI--LNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENAR--------LTFSVMKKN--------------NKPTEFDSSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV------KDLNWAVPRLTIGTPDLSLDEVLLRLNANVAKQQ 2469 LP K+L E F+ LE ++ NR + T T ++ V+ + R F E LG+I AVYP AY+ R + + + S+ ++ + +RP L +S L +R F +N V+IV ++ F+ LN I+ + + W+ F+++ +V V+P +PQ Q ++L +A+ L M+K +P S V+P +T L +SQSL++++R +A + +V+T P R M S+LPE+A I+ N F + + KP + ++ L C NR+ I Y R D ++L +L ++ P W+ KDL + + T DLS+ VL +LN +++ Sbjct: 321 LPYQYKLLAEMFRSLETIVAMLFNRSETVTFTKVKQGVQDMMRKR-FEESHLGQIKAVYPSAYTFRQEKNIISFSATAKRSSYQLTVEPVIDEEFKGVRPVLSASRLL--------ERRHIFHQNLVEIVKGHHKTFLASLNPPIVVPDE-----KLTRWHPRFSVD-EVPNVKPSDLPQPPQTEKLTSAQEVLDKARALMTPKMEKALANMALKTAEKACVKEPETTAKSAVTPTETPGA--LKGVSQSLLERIRAKEAQKLHAVMTRNPQQEERLLMMSRLPELARILRNVF-VAEKKP---ALIMELAC--------NRM---IASY---RSPLTSD--EMEKHLRLLAELTPAWLTIHPIRKDLYLKLNKTT----DLSI--VLDKLNQKTKEEE 676
BLAST of SMED30018579 vs. TrEMBL
Match: C1L4L6 (DNA replication factor Cdt1 (Double parked homolog) OS=Schistosoma japonicum OX=6182 GN=DUP PE=2 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 4.960e-12 Identity = 95/420 (22.62%), Postives = 171/420 (40.71%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELE---KLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLE-----------SSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELE-----------NARLTF---------SVMKKNNKPTEF-DSSKVSPLKT---------GDN---------------------HRLGSLSQSLIDKVRMNKANRSVIT----GAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVLLRLNANVAK 2463 LP +L+VL E F+ + ++++ C+ L+ AV+ + R F E +G+ L +YP Y +R + K RS+ N + + + PNL S FL F + +R +F + +V+ S+REF++++ I + E W+ F +E V ++ P P+P + + E + AR F S+ K+ P SPLK DN + L +S +L++KVR + R ++T G + RR +LP I T + + + + +P+ S + V + + + SE+LD+L ++ P W++ LNW+ L + P+ S+ +V+ + VAK Sbjct: 177 LPHNLRVLLELFRSCDTVVSMLHNREELCSFDKLQPAVQEIV-RRNFEETHVGQFLTIYPMVYFLRYEKQLDKFTHRSNGNYV-LVLSPNLRTDGTQIGHDSPSKGFLPFTGTRLIQRRNRFHSLLLNLVLKSHREFLISELGIDPSKLPEDSSLRRWHPKFPLESAVTSILPSPLPVRPSEFEQKITSAKDAVKAFQARALFREAETCHQISLSKQQLSPIPSPKKPAASPLKASNIIGSTVCADNVMGTQTNSEESTSKRIITSTPNTLKGVSLALLNKVRARENERRLLTELTYGKVSETRRAIYC-RLPLIVTQVWSILRSSNARPVPLSTVAVRVADAHPSG--------------------ISADAISEHLDVLVELAPCWIEKLNWSTVHLRLKDPERSVKDVIDLVKMKVAK 573
BLAST of SMED30018579 vs. Ensembl Cavefish
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:ZFIN;Acc:ZDB-GENE-070111-1]) HSP 1 Score: 67.0106 bits (162), Expect = 6.193e-11 Identity = 79/355 (22.25%), Postives = 156/355 (43.94%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLI--YDNRRKCAT-TYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQ-HVPS-KNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDN--------------------HRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWV 2367 LP K+L E F+ ++ ++ NR + T ++ V+ + H++F E +G+I VYP AY+ R + ++P+ + S ++ + +RP L ++ L R + F +N V+IV ++ F+ + + VE ++ I W+ F+++ +V ++P +PQ Q ++L A+ + P + LKT + L +SQSL++++R +A + +T P R M S+LPE+A I+ N F + + KP + ++ + C NR ++ + G ++L +L ++ P W+ Sbjct: 310 LPYKYKLLAEMFRSMDTIVGMLFNRSETVTFAKVKQGVQDMM-HKRFEEAHIGQIKTVYPSAYTFRQEKNIPNFSTTAKKSAYQLTVEPVIEEETNGVRPVLSATRLL--------DRRRMFHQNLVEIVKGHHKVFLAGLNPL-VEVPDDKI--TRWHPRFSVD-EVPNIQPGDLPQPPQTEKLTTAQEVLDRARALMTPKMEKALANMALKTAEASCAKEPEPPAKPVETPAQTPSALKGVSQSLLERIRAKEAQKLQVAMTRNPEQEERLGMMSRLPELARILRNVF-VAEKKP---ALIMEIAC--------NR--------VVASYRSSLSSGEMEKHLRLLAELAPEWL 631
BLAST of SMED30018579 vs. Ensembl Medaka
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:101175588]) HSP 1 Score: 67.781 bits (164), Expect = 2.958e-11 Identity = 69/291 (23.71%), Postives = 136/291 (46.74%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSN--HRQFTECQLGKILAVYPEAYSVRLQ-HVPS-KNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIEL-DVKTVEPVPMPQ-----KSQQKELENARLTF----------SVMKKNNKPTEFD------SSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEF 2172 LP H ++L E F+ ++ ++ +C T + + + HR+F + + +I V+PEAY+++ + ++P+ +G++ ++ + RP L +S L +R + F +N + +V ++EF+ S++ +V E R W+ FN++L V P+P P + Q+ L+ ARL + + +P + + V+P+ L +SQSL+D++R +A + +V+T PA R M S+L ++A I+ F Sbjct: 173 LPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQEKNIPTFSSGIKKGEYQLTVEPIIVSDHSQARPVLSASLLL--------ERRRVFHQNLISLVKQHHQEFL--SSLVPPVSVPED-RLTRWHPRFNVDLVPAVPVSPLPEPPHRETLSTAQEVLDRARLLINPKMEKALISAAQQVQPRPAQGSVCAQKAAEPVAPVPGS----LKGVSQSLLDRIRAKEAQKLQAVMTRDPAHEERLLMMSRLVDLARILRGIF 448 HSP 2 Score: 53.5286 bits (127), Expect = 7.124e-7 Identity = 47/199 (23.62%), Postives = 86/199 (43.22%), Query Frame = 1 Query: 166 SYQSLNTI--------LIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNK-------------SDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLE-TSVQPIEPI 696 +YQ +T+ ++P+HF+ + ++F S+D VV L + + KV + +++M F+ QI +FPE+Y+ + + K IP F+ G K SD +++ V L+ R ++ L+ V++ H EFL V + T +WH FN++ P+ P+ Sbjct: 156 AYQRYHTLAQDIPPGLVLPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQE--------------KNIPTFSSGIKKGEYQLTVEPIIVSDH--SQARPVLSASLLLERRRVFHQNLISLVKQHHQEFLSSLVPPVSVPEDRLT---RWHPRFNVDLVPAVPVSPL 335
BLAST of SMED30018579 vs. Ensembl Medaka
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:101175588]) HSP 1 Score: 67.0106 bits (162), Expect = 6.098e-11 Identity = 69/291 (23.71%), Postives = 136/291 (46.74%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSN--HRQFTECQLGKILAVYPEAYSVRLQ-HVPS-KNGLRSSTNKIDI---------AIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIEL-DVKTVEPVPMPQ-----KSQQKELENARLTF----------SVMKKNNKPTEFD------SSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANR--SVITGAPATIRRNFMASKLPEIATIIANEF 2172 LP H ++L E F+ ++ ++ +C T + + + HR+F + + +I V+PEAY+++ + ++P+ +G++ ++ + RP L +S L +R + F +N + +V ++EF+ S++ +V E R W+ FN++L V P+P P + Q+ L+ ARL + + +P + + V+P+ L +SQSL+D++R +A + +V+T PA R M S+L ++A I+ F Sbjct: 223 LPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQEKNIPTFSSGIKKGEYQLTVEPIIVSDHSQARPVLSASLLL--------ERRRVFHQNLISLVKQHHQEFL--SSLVPPVSVPED-RLTRWHPRFNVDLVPAVPVSPLPEPPHRETLSTAQEVLDRARLLINPKMEKALISAAQQVQPRPAQGSVCAQKAAEPVAPVPGS----LKGVSQSLLDRIRAKEAQKLQAVMTRDPAHEERLLMMSRLVDLARILRGIF 498 HSP 2 Score: 54.299 bits (129), Expect = 5.068e-7 Identity = 47/199 (23.62%), Postives = 86/199 (43.22%), Query Frame = 1 Query: 166 SYQSLNTI--------LIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNK-------------SDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLE-TSVQPIEPI 696 +YQ +T+ ++P+HF+ + ++F S+D VV L + + KV + +++M F+ QI +FPE+Y+ + + K IP F+ G K SD +++ V L+ R ++ L+ V++ H EFL V + T +WH FN++ P+ P+ Sbjct: 206 AYQRYHTLAQDIPPGLVLPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQE--------------KNIPTFSSGIKKGEYQLTVEPIIVSDH--SQARPVLSASLLLERRRVFHQNLISLVKQHHQEFLSSLVPPVSVPEDRLT---RWHPRFNVDLVPAVPVSPL 385
BLAST of SMED30018579 vs. Planmine SMEST
Match: SMESG000017019.1 (SMESG000017019.1) HSP 1 Score: 970.304 bits (2507), Expect = 0.000e+0 Identity = 517/542 (95.39%), Postives = 519/542 (95.76%), Query Frame = 1 Query: 853 GIINKGKMSMTNLSSTNGKENKNQIKWSNNEQISPTXXXXXXXXXXXXXXXXXXXXXXPSNQNAANEXXXXXXXXXXXXXQRKSSKLTVRFSTEDARQSNSDRKAFQSINRIPLRKSAITPSKTTPKRSENPPSVPNSGSKPTRSQPRLSFISNSNGGTPNGKNFLASVAMPNSPMLKTEFVLKQKLPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANRSVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVLLRLNANVAKQQPLK 2478 GIINKGKMSMTNLSSTNGKENKNQIKWSNNEQISPTILKEKLIKKSSLSNENNSNDLKPSNQNAANESSKSK+SPIDKSIQRKSSKLTVRFSTEDARQSNSDRKAFQSINRIPLRKSAITPSKTTPKRSENPPSVPNSGSKPTRSQPRLSFISNSNGGTPNGKNFLASVAMPNSPMLKTEFVLKQKLPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKN KPTEFDSSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANRSVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNES SFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPD+SLDEVLLRLNANVAKQQPLK Sbjct: 394 GIINKGKMSMTNLSSTNGKENKNQIKWSNNEQISPTILKEKLIKKSSLSNENNSNDLKPSNQNAANESSKSKESPIDKSIQRKSSKLTVRFSTEDARQSNSDRKAFQSINRIPLRKSAITPSKTTPKRSENPPSVPNSGSKPTRSQPRLSFISNSNGGTPNGKNFLASVAMPNSPMLKTEFVLKQKLPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSNHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSNFLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKKNKKPTEFDSSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANRSVITGAPATIRRNFMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNES----------------------SFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDVSLDEVLLRLNANVAKQQPLK 913 HSP 2 Score: 508.449 bits (1308), Expect = 7.323e-167 Identity = 295/309 (95.47%), Postives = 299/309 (96.76%), Query Frame = 1 Query: 28 MTADQIAKPSLNSLNVQSNIXXXXXXXXXXXXXXXXXXXXXXNNDSSYQSLNTILIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLETSVQPIEPITLDRNNLKQLSKKSSDKIAKSPLSASNNKRTLEXXXXXXXXXXXXXXXXXXXGIINKGKMSMTNLSSTNGKENKNQIKWSNNEQIS 954 MTADQIAKPSLNSLNVQSNISKKADKTSSAKSKVKKTC TVSNNDSSYQSLNTILIP HFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSK EMEMFTLNK WHEDFNLETSVQPIEPITLDRNNLKQLSKKSSDKIAKSPLSASNNKRTLENVKSTSKS LSLSNRS SVGIINKGKMSMTNLS+T+GKENKN IK NN+ +S Sbjct: 1 MTADQIAKPSLNSLNVQSNISKKADKTSSAKSKVKKTCATVSNNDSSYQSLNTILIPAHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKEEMEMFTLNKIWHEDFNLETSVQPIEPITLDRNNLKQLSKKSSDKIAKSPLSASNNKRTLENVKSTSKSPLSLSNRSSSVGIINKGKMSMTNLSATDGKENKNCIKSLNNQHLS 309 HSP 3 Score: 53.1434 bits (126), Expect = 9.175e-7 Identity = 27/34 (79.41%), Postives = 29/34 (85.29%), Query Frame = 1 Query: 853 GIINKGKMSMTNLSSTNGKENKNQIKWSNNEQIS 954 GIINKGKMSMTNLSSTNGKENKN I NN+ +S Sbjct: 335 GIINKGKMSMTNLSSTNGKENKNPIISVNNQYLS 368 HSP 4 Score: 50.447 bits (119), Expect = 6.286e-6 Identity = 61/245 (24.90%), Postives = 102/245 (41.63%), Query Frame = 1 Query: 1411 LPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLS--NHRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLE---SSNFLK------FDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQ---KSQQKELENARLTFSVMKKNNKPT--EFDSSKVSPLKTGDNHRLGSLSQSLIDKVRMNKANRSVITG 2097 +PAH K + + F L+ ++ ++ + Y+ E L N F G+IL ++PE+YS P K + K + I PN S+F+ F + R E + V + EF+L + E +E + W+ DFN+E V+ +EP+ + + K K+ + + NNK T S+ SPL + S S +I+K +M+ N S G Sbjct: 56 IPAHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLK---VTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKEEMEMFTLNKIWHEDFNLETSVQPIEPITLDRNNLKQLSKKSSDKIAKSPLSASNNKRTLENVKSTSKSPLSLSNR----SSSVGIINKGKMSMTNLSATDG 293
BLAST of SMED30018579 vs. Planmine SMEST
Match: SMESG000017018.1 (SMESG000017018.1) HSP 1 Score: 186.422 bits (472), Expect = 1.184e-49 Identity = 128/369 (34.69%), Postives = 191/369 (51.76%), Query Frame = 1 Query: 1390 EFVLKQKLPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSN-HRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSN-----------FLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQ----KELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDNHR---LGSLSQSLIDKVRMNKANRSVITGAPATIRRNF-MASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVL 2436 E LKQKL HLK L+E F +L+K+++D K +L V LS + F+E LGKI+ +Y EAY ++ + +PS N + KIDI I+PNL + N FL+FDF +K+R FLE C++ V SYR F+LNK I E + +++ E W+C+FNI+L V+ V+P PMP + ++ KE+ + + +MKK N FD L+ N +L QS+IDKV NK R N + SKL ++ ++ EFK KPL S L N+ SL ++E+ F +YL L ++VPGW+++L P+L+I + S+D+VL Sbjct: 279 EHSLKQKLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKP-VKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCIT-PELINDYLLNESWHCEFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKN---VFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAVVSKLRDLVALLYTEFKAVQYKPLAFSYLSNIASVSLAVDEN----------------------YFIDYLCYLMKLVPGWIEELGGPPPQLSIDKSNFSIDDVL 620 HSP 2 Score: 144.05 bits (362), Expect = 1.802e-35 Identity = 79/186 (42.47%), Postives = 119/186 (63.98%), Query Frame = 1 Query: 178 LNTILIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFL----LINK----NLSKVEMEMFTLNKKWHEDFNLETSVQPIEPITLDRN 711 +N + +P+HFK +LD+FVSLD VNKL S+YI+++ EVLK MN ++F+ IF QIL IFPESY YEF+S P+K+ ++++E++K++PN +FG D P+K +K + Q+ R + LL V+++H+ FL IN NL KV+ +WH F+LE V+ I+P+ LDRN Sbjct: 34 MNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMN-HDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINDKHEGNLIKVQ--------EWHCKFDLENCVEEIQPVFLDRN 210 HSP 3 Score: 51.6026 bits (122), Expect = 2.624e-6 Identity = 41/168 (24.40%), Postives = 79/168 (47.02%), Query Frame = 1 Query: 202 HFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKII--PNFNFGNKSDFIPKKSQKVFRLD--QLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLETSVQPIEP 693 H K + ++F+ LD VV+ + + ++ ++ L +N + F G+I+ I+ E+Y +++ P + +K+ I PN N + + R D QL R E + V + FLL NK ++ + + LN+ WH +FN++ V+ ++P Sbjct: 289 HLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVN-SPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLL-NKCITPELINDYLLNESWHCEFNIDLHVELVDP 454
BLAST of SMED30018579 vs. Planmine SMEST
Match: SMESG000017018.1 (SMESG000017018.1) HSP 1 Score: 186.422 bits (472), Expect = 1.259e-49 Identity = 128/369 (34.69%), Postives = 191/369 (51.76%), Query Frame = 1 Query: 1390 EFVLKQKLPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSN-HRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSN-----------FLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQ----KELENARLTFSVMKKNNKPTEFDSSKVSPLKTGDNHR---LGSLSQSLIDKVRMNKANRSVITGAPATIRRNF-MASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESNRIELFIYMYIIQRCHFVMDLGSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDLSLDEVL 2436 E LKQKL HLK L+E F +L+K+++D K +L V LS + F+E LGKI+ +Y EAY ++ + +PS N + KIDI I+PNL + N FL+FDF +K+R FLE C++ V SYR F+LNK I E + +++ E W+C+FNI+L V+ V+P PMP + ++ KE+ + + +MKK N FD L+ N +L QS+IDKV NK R N + SKL ++ ++ EFK KPL S L N+ SL ++E+ F +YL L ++VPGW+++L P+L+I + S+D+VL Sbjct: 278 EHSLKQKLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKP-VKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCIT-PELINDYLLNESWHCEFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKN---VFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAVVSKLRDLVALLYTEFKAVQYKPLAFSYLSNIASVSLAVDEN----------------------YFIDYLCYLMKLVPGWIEELGGPPPQLSIDKSNFSIDDVL 619 HSP 2 Score: 144.05 bits (362), Expect = 1.740e-35 Identity = 79/186 (42.47%), Postives = 119/186 (63.98%), Query Frame = 1 Query: 178 LNTILIPVHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFL----LINK----NLSKVEMEMFTLNKKWHEDFNLETSVQPIEPITLDRN 711 +N + +P+HFK +LD+FVSLD VNKL S+YI+++ EVLK MN ++F+ IF QIL IFPESY YEF+S P+K+ ++++E++K++PN +FG D P+K +K + Q+ R + LL V+++H+ FL IN NL KV+ +WH F+LE V+ I+P+ LDRN Sbjct: 33 MNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMN-HDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINDKHEGNLIKVQ--------EWHCKFDLENCVEEIQPVFLDRN 209 HSP 3 Score: 51.6026 bits (122), Expect = 2.556e-6 Identity = 41/168 (24.40%), Postives = 79/168 (47.02%), Query Frame = 1 Query: 202 HFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKII--PNFNFGNKSDFIPKKSQKVFRLD--QLVSRMAHLYECLLGKVQERHNEFLLINKNLSKVEMEMFTLNKKWHEDFNLETSVQPIEP 693 H K + ++F+ LD VV+ + + ++ ++ L +N + F G+I+ I+ E+Y +++ P + +K+ I PN N + + R D QL R E + V + FLL NK ++ + + LN+ WH +FN++ V+ ++P Sbjct: 288 HLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVN-SPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLL-NKCITPELINDYLLNESWHCEFNIDLHVELVDP 453 The following BLAST results are available for this feature:
BLAST of SMED30018579 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of SMED30018579 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30018579 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30018579 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of SMED30018579 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of SMED30018579 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30018579 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of SMED30018579 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30018579 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of SMED30018579 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30018579 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30018579 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30018579 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of SMED30018579 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30018579 ID=SMED30018579|Name=SMED30018579|organism=Schmidtea mediterranea sexual|type=transcript|length=2562bpback to top protein sequence of SMED30018579-orf-1 >SMED30018579-orf-1 ID=SMED30018579-orf-1|Name=SMED30018579-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=818bp MTADQIAKPSLNSLNVQSNISKKADKTSSAKSKVKKTCVTVSNNDSSYQSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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