tRNA pseudouridine synthase A

Overview
NametRNA pseudouridine synthase A
Smed IDSMED30018566
Length (bp)1353
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA pseudouridine synthase A (SMED30018566) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA pseudouridine synthase A (SMED30018566) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA pseudouridine synthase A (SMED30018566) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA pseudouridine synthase A (SMED30018566) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018566

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30018566SMESG000053891.1 SmedASXL_009187SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
cephalic gangliaSMED30018566SMESG000053891.1 dd_Smed_v4_8828_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30018566SMESG000053891.1 dd_Smed_v4_8828_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018566SMESG000066407.1 SMESG000053891.1 SMESG000046471.1 SMESG000041187.1 Contig41508newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018566SMESG000066407.1 SMESG000053891.1 SMESG000046471.1 SMESG000041187.1 Contig41508uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
zeta neoblastSMED30018566SMESG000053891.1 dd_Smed_v4_8828_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Match: PUSL1 (pseudouridine synthase like 1 [Source:HGNC Symbol;Acc:HGNC:26914])

HSP 1 Score: 62.003 bits (149), Expect = 4.525e-10
Identity = 63/297 (21.21%), Postives = 123/297 (41.41%), Query Frame = 2 
Query:   29 QFMLHISYVGTQYQGIS--KSSKRAV-ISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGL---------ISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNL---NITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKN 865
            +++++  YVGT + G++  + ++RAV +    E+  + L   +P     SSRTD GVHA  N         +    F  + L   +N +L   +  IR+ +   +P D +       RTYLYR+           +   N  W                  +     E A+ L GT+++  F +    +    PV+ L   +++  +++P V    S         +++ ++   ++SF+ + VR +   ++ V +G      V  ++      L +     AP + L+L ++ Y N
Sbjct:   13 RYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFTISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPA--IRVLRAFRVPSDFHARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADC-----------LDMVAMQEAAQHLLGTHDFSAFQSAGSPV--PSPVRTLRRVSVSPGQASPLVTPEESR-------KLRFWNLEFESQSFLYRQVRRMTAVLVAVGLGALAPAQVKTILE-SQDPLGKHQTRVAPAHGLFLKSVLYGN 286
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Fly
Match: CG34140 (gene:FBgn0083976 transcript:FBtr0110975)

HSP 1 Score: 65.4698 bits (158), Expect = 1.625e-11
Identity = 73/315 (23.17%), Postives = 135/315 (42.86%), Query Frame = 2 
Query:   29 QFMLHISYVGTQYQGISKS---------SKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVR--FLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCA----------TAWESQQIKFV-GAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKD-------PVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKP--------FICAPPYALYLYNIEYK 862
            +++L+ISY+GT ++GI K+           +++   +E  L    P N+   +  SSRTD GVHA  +TV+      +   +++  L   +N  L++    IR+   K +    +   +   RTYLYR   ++    L +C+             E  +  F+  + F+ +     A+   G +++  F ++      +D        +  +NI      P      S   + +     Y D+ + A+SF+ K VR +VG++I  A+GN R+++      R  Y +   P         + AP   LYL  + Y+
Sbjct:    3 RYLLNISYIGTTFRGIQKTVNKLEQSRLDTKSIQGCLELALQVFHPTNEIHTV-LSSRTDAGVHALHSTVQVDLERPNGQPYDTTILTGVLNRTLNKQRLPIRVLSSKLVANSFHCRYDAIGRTYLYRFA-VAKVPTLGDCSLRNRSFETFIPVEEIDRCYFLQSSSFDIKRVQAAARMFIGVHDFRTFMSVSRQKVCRDHPMFTVRKIDEINIR-----PGETRALSSNAIQAAETYNYWDIEIRAKSFLYKQVRRIVGALI--ALGNGRIDE------RCLYQMLTVPSKNSWDHRVLLAPACGLYLCRVHYR 302
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 79.337 bits (194), Expect = 5.065e-16
Identity = 70/304 (23.03%), Postives = 132/304 (43.42%), Query Frame = 2 
Query:   17 RNLKQFMLHISYVGTQYQGISKS-SKRAVISV---IEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYS---FNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCAT---AWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQ 898
            + + ++++   Y G++Y G+ K+ + +AV  V   +E  + KL P N+   +  SSRTD GVHA  N+              + + L   +N+ L    E IRI +   +  D +       RTY+YR+ L    + L         W  +  +      N +   E +  L GT+++  F  L      K PVK L++   E   S    H          +++ ++   + SF+ + VR + G ++ +  G   +  + D++      L     + AP + L+L N++Y++  L   +++ Q
Sbjct:    3 QTVARYLIFFQYTGSKYSGVMKAPAHQAVQGVGNHLENAVRKLKPVNE-VCVSISSRTDTGVHALCNSAHVDIQRRADKPPLSEQSLLEALNFQLK--EEPIRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTRL-----NIDAMREASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQR------DIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSVSKIQDLLE-ARDSLAFPNNLTAPAHGLFLTNVQYRDTDLVASMSEEQ 291
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 78.5666 bits (192), Expect = 8.712e-16
Identity = 68/293 (23.21%), Postives = 127/293 (43.34%), Query Frame = 2 
Query:   17 RNLKQFMLHISYVGTQYQGISKS-SKRAVISV---IEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYS---FNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCAT---AWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKN 865
            + + ++++   Y G++Y G+ K+ + +AV  V   +E  + KL P N+   +  SSRTD GVHA  N+              + + L   +N+ L    E IRI +   +  D +       RTY+YR+ L    + L         W  +  +      N +   E +  L GT+++  F  L      K PVK L++   E   S    H          +++ ++   + SF+ + VR + G ++ +  G   +  + D++      L     + AP + L+L N++Y++
Sbjct:    3 QTVARYLIFFQYTGSKYSGVMKAPAHQAVQGVGNHLENAVRKLKPVNE-VCVSISSRTDTGVHALCNSAHVDIQRRADKPPLSEQSLLEALNFQLK--EEPIRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTRL-----NIDAMREASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQR------DIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSVSKIQDLLE-ARDSLAFPNNLTAPAHGLFLTNVQYRD 280
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 55.4546 bits (132), Expect = 1.806e-8
Identity = 45/206 (21.84%), Postives = 85/206 (41.26%), Query Frame = 2 
Query:  266 MNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCAT---AWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHL 874
            +N+ L    E IRI +   +  D +       RTY+YR+ L    + L         W  +  +      N +   E +  L GT+++  F  L      K PVK L++   E   S    H          +++ ++   + SF+ + VR + G ++ +  G   +  + D++      L     + AP + L+L N++Y++  L
Sbjct:   15 LNFQLK--EEPIRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTRL-----NIDAMREASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQR------DIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSVSKIQDLLE-ARDSLAFPNNLTAPAHGLFLTNVQYRDTDL 206
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: PUSL1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:100497905])

HSP 1 Score: 74.3294 bits (181), Expect = 3.681e-14
Identity = 68/289 (23.53%), Postives = 118/289 (40.83%), Query Frame = 2 
Query:   29 QFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKT---SSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLN 877
            ++++   Y GT+Y G+ ++     +  ++  L     + +P G      SSRTD GVHA  N+        N    F    L   +N++L    E IR+ +   +  + +       RTY+YR+    + + L      +E      V    N     E ++ L GT+N+  F +       K P+K L       +  +L  H          +++ D    A SF+ K VR + G+++ V  G      + + +           F+ APP+ L+L N+EY    LN
Sbjct:    7 RYLVFFQYYGTKYSGVMETPVTQSVLGVQNYLEMAAQKLRPVGTVKFFISSRTDSGVHAICNSAHVDIQRANGKPPFTEDILVQALNHHLQ--PEPIRLLKAVRVCDNFHARHKALSRTYVYRVAAGCSHSEL----PVFEENLCWAVPGSLNVAAIHEASEMLLGTHNFSAFRSANSENVFKSPIKTLQQADITPSSGILTHHWQ-----YRNLQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPLQIKEFLENQDPNCTLGNFV-APPHGLFLNNVEYDETELN 283
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Match: PUSL1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:100497905])

HSP 1 Score: 71.633 bits (174), Expect = 2.407e-13
Identity = 67/283 (23.67%), Postives = 117/283 (41.34%), Query Frame = 2 
Query:   29 QFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKT---SSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEY 859
            ++++   Y GT+Y G+ ++     +  ++  L     + +P G      SSRTD GVHA  N+        N    F    L   +N++L    E IR+ +   +  + +       RTY+YR+    + + L      +E      V    N     E ++ L GT+N+  F +       K P+K L       +  +L  H       N  +++ D    A SF+ K VR + G+++ V  G      + + +           F+ APP+ L+L N+EY
Sbjct:    7 RYLVFFQYYGTKYSGVMETPVTQSVLGVQNYLEMAAQKLRPVGTVKFFISSRTDSGVHAICNSAHVDIQRANGKPPFTEDILVQALNHHLQ--PEPIRLLKAVRVCDNFHARHKALSRTYVYRVAAGCSHSEL----PVFEENLCWAVPGSLNVAAIHEASEMLLGTHNFSAFRSANSENVFKSPIKTLQQADITPSSGILTHHWQ---YRN--LQFWDFTFRARSFLYKQVRRMTGALVAVGQGRLTPLQIKEFLENQDPNCTLGNFV-APPHGLFLNNVEY 277
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Match: Pusl1 (pseudouridylate synthase-like 1 [Source:MGI Symbol;Acc:MGI:3047787])

HSP 1 Score: 56.225 bits (134), Expect = 2.730e-8
Identity = 63/296 (21.28%), Postives = 122/296 (41.22%), Query Frame = 2 
Query:   29 QFMLHISYVGTQYQGIS--KSSKRAV--ISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTN---SHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGL---------ISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEG-MLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKN 865
            ++++ + Y+GT + G++  + + RAV  ++ +EE   K L    P     SSRTD GVHA  N              F+ + +   +N +L   +  IR+ +   +P D +       RTY YR+           +   N  W   T +            +     E A+ L GT+++  F +    + N    ++ ++++   ++P VL   S         +++  +   ++SF+ + VR +   ++ V +G      V  V+      L +     AP   L+L ++ Y N
Sbjct:   13 RYLVFLQYLGTDFNGVAAVRGNPRAVGVLNFLEEAA-KRLNSVDPVRFTISSRTDAGVHALSNAAHLDIQRRPGQSPFSPEVVAKALNTHLKHPA--IRVLKAFRVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEY-----------LDMAAMQEAAQHLLGTHDFSAFQSAGSPVTNTVRTLRRVSVSPGPASPFVLPEGSR-------RLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQV-KVILESQDPLGKYQARVAPARGLFLKSVLYDN 286
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|A9ILJ7|TRUA_BART1 (tRNA pseudouridine synthase A OS=Bartonella tribocorum (strain CIP 105476 / IBS 506) OX=382640 GN=truA PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.111e-18
Identity = 68/283 (24.03%), Postives = 123/283 (43.46%), Query Frame = 2 
Query:   23 LKQFMLHISYVGTQYQGISKSSKRAVI-SVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNT--VRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLN-ITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEY 859
            + +F L + Y G+ Y G  + +K   I   +E+ + +     +   + T+ RTD GVHA      V F+ N    +    +R  +N  L + SE I I  V+ +P D +   +  +R YL++I L   S    N    W      ++  P N +   E A+ L G +++  F +      AK P++ L  + + +    +                   ++  A SF++  +R+  GS+++V IG    +D+   +   H    ++  + APP  LYL  +EY
Sbjct:    1 MSRFKLTLEYDGSNYAGWQRQAKLHTIQGALEQAIFRF--SGQQLTITTAGRTDAGVHATGQVAHVDFIKN----WPPHTVRDALNALLRQQSEAISILNVENVPDDFDARFSAIKRHYLFKI-LNRRSPPALNAKRVW------WIPKPLNAQAMHEAAQKLVGQHDFTTFRS--AHCQAKSPIRTLERLDIQKEGEEIF------------------IYAKARSFLHHQIRSFAGSLMEVGIGRWTAQDLEAAL---HAKDRKRCGVVAPPSGLYLTKVEY 247
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|B8E1G5|TRUA_DICTD (tRNA pseudouridine synthase A OS=Dictyoglomus turgidum (strain Z-1310 / DSM 6724) OX=515635 GN=truA PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.050e-17
Identity = 68/274 (24.82%), Postives = 119/274 (43.43%), Query Frame = 2 
Query:   38 LHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEY 859
            L ISY+G  + G  K   +  I    E++L  L + +   +  + RTD GVHA    V F +  H  F+   L   +N  L    E I+I +V+ +    +   +  RR Y+Y +      +      + W S++I       N EL + +A    G +++                KN  +T  E    +    S      +  +     F++A  F+ KMVR +VGSM+++A+G   + ++ D +        E+  + APP+ LYL+ ++Y
Sbjct:    6 LEISYIGKDFYGFQKQPGKRTIQGELEKVLSFLFDEE-IRVIGAGRTDTGVHALGQVVNFKSRGHKGFSCDKLHKVLNRLLP---EDIKIKKVEIVDDSFHARYSAKRRWYIYVVYNNDERDLFLKDYSWWISREI-------NRELLIFSANLFKGVHDF----------------KNFCVTEDEDQTVIEVYESFWYFKKDLLI----YFISAPFFLRKMVRFIVGSMVEIALGRKSLIELEDYLKEER---KERFSVPAPPWGLYLFRVDY 245
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|Q6G547|TRUA_BARHE (tRNA pseudouridine synthase A OS=Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) OX=283166 GN=truA PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.392e-17
Identity = 68/282 (24.11%), Postives = 121/282 (42.91%), Query Frame = 2 
Query:   23 LKQFMLHISYVGTQYQGISKSSK-RAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNT--VRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEY 859
            + +F L + Y G+ Y G  + ++ R + S +E+ L       +   + T+ RTD GVHA      V F  N    + +  +R  +N +L +  + I I  V+ +P   +   +  +R YL++I L   S    N    W      ++  P N +   E A+ L G +++  F +      AK P++ L                   L      + I ++  A SF++  +R+  GS+++V IG    +D+   +   H     +  + APP  LYL  +EY
Sbjct:    1 MPRFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHF--SGQQLTITTAGRTDAGVHATGQVAHVDFEKN----WRTHTVRDALNAHLQKQGDNIAILHVQNVPDSFDARFSAIKRHYLFKI-LNRRSPPALNTKRVW------WIPKPLNAQAMHEAAQKLVGKHDFTTFRS--AHCQAKSPIRTLE-----------------RLDVQREGEEIFLYAQARSFLHHQIRSFAGSLMEVGIGRWTTQDLEAAL---HAKDRTRCGMVAPPSGLYLTKVEY 247
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|B4U9D9|TRUA_HYDS0 (tRNA pseudouridine synthase A OS=Hydrogenobaculum sp. (strain Y04AAS1) OX=380749 GN=truA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.570e-16
Identity = 70/256 (27.34%), Postives = 117/256 (45.70%), Query Frame = 2 
Query:   23 LKQFMLHISYVGTQYQGISKSSKRAVIS-VIEEQLLKLLPENKPFGLKTS----SRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLIS-NSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSD 772
            +K +   IS+VGT Y G         I   +EE+L  ++ + KP   K      SRTD+GVHA    + F+ N    F+ KDL +      S   + I+IH ++E+PL+ N   +  ++TY+Y++     NS F  + A          V  P +    +E +    G  ++ GFT      N    V+N+   V                  ++P+  +++ +TA  F+  MVR +VG+M+  A G   ++DV+D
Sbjct:    1 MKNYKFTISFVGTNYSGWQYQPNVPTIQNEVEEKLYYIVNKKKPIKQKIRAIGCSRTDKGVHA----IEFVFNVKMEFD-KDLNFLRKALNSALPKDIKIHNIEEVPLEFNSRFDALKKTYIYKLFFDEKNSPFFQDRAM--------LVYKPTDLGKMMEISHLFLGYKDFRGFTKELEDENGYCSVENITFKV------------------DYPL--VEISITANRFLRYMVRRMVGTMLSYAQGLISIDDVND 223
[Close]
BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Match: sp|Q92SH4|TRUA_RHIME (tRNA pseudouridine synthase A OS=Rhizobium meliloti (strain 1021) OX=266834 GN=truA PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.436e-16
Identity = 72/282 (25.53%), Postives = 124/282 (43.97%), Query Frame = 2 
Query:   23 LKQFMLHISYVGTQYQGISKSS-KRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF-LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLN-ITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEY 859
            + ++ L + Y G+ Y G  +     +V   IE+ +L L  E     ++ + RTD GVHA        LT    S+    L+  +N +L+ A E + I  V E P D +   +  RR YLYRI  IS  + L     A E+++  +V  P + +   E A+ L G +++  F +      A  P++ L+ + VT +                   + I++  TA+SF++  +R+  GS+  V  G    +D+   +        +     APP  LY   ++Y
Sbjct:    1 MPRYRLTVEYDGSDYVGWQRQDNGPSVQGAIEKAVLSLTRET--VSIRGAGRTDSGVHARGQVAHLDLTREWKSYT---LQNALNAHLALAGERVSILDVAEAPGDFDARFSAIRRHYLYRI--ISRRSPL-----ALEARRAWWVPKPLDHDAMHEAAQRLVGHHDFTTFRS--AHCQATSPLRTLDRLDVTRAG------------------ELIEIRATAQSFLHNQIRSFAGSLKLVGEGKWTPDDLQAALE---ARDRKACGPVAPPDGLYFMRVDY 247
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A1I8HE35 (tRNA pseudouridine synthase OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.851e-38
Identity = 97/315 (30.79%), Postives = 159/315 (50.48%), Query Frame = 2 
Query:    5 SQKCRNLKQFMLHISYVGTQYQGISKSSK----RAVISVIEEQLLKLL-PENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPL---DMNIISNVFRRTYLYRIGLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNIT 922
            S K     Q +L++SY G QY G +  S     R+V   +   L++L+ P      L  SSRTD+ VHA  NTV    +    ++   LR  +N +L ++ E IRIH ++ +PL   + ++++ V RR Y+YR+ L++     L +   AW       V  P + EL  + A  L+  +++  FTNL G+     P++ L I V +S  SV  +H+    +S+     +D+   A SF+ K VR + G+++  A G   ++ + D+++    Y   KP+  APP+ LYL  + Y N    +D+N W D    N T
Sbjct:   41 SDKTAGRSQLLLYVSYNGCQYNGAANHSSDWQVRSVSKALHHHLIQLMGPSRWINRLNISSRTDKDVHALMNTVSVRHSGPIDYSVALLRLMLNASLKQSGESIRIHLIRPVPLSSGEFSLVTGVARREYVYRLALLAEPGLTLMHPEAAWLGSSRWLVEPPLSLELLQQAAGVLNEEHDFSAFTNLNGLSRCYTPLRRLEICVDDSV-SVDRLHACNSFTSDCFTGCVDLHFGARSFLYKQVRFMAGALVATAKGTVTLDQLKDMLSNPSDYTGLKPYDAAPPHGLYLKCVRYANP--KWDLN-WPDIQADNAT 351
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A267FZ26 (tRNA pseudouridine synthase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031552g1 PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 6.181e-33
Identity = 81/259 (31.27%), Postives = 134/259 (51.74%), Query Frame = 2 
Query:  158 LKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPL---DMNIISNVFRRTYLYRIGLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNIT 922
            L  SSRTD+ VHA  NTV    +    ++   LR  +N +L ++ E IRIH ++ +PL   + ++++ V RR Y+YR+ L++     L +   AW       V  P + EL  + A  L+  +++  FTNL G+     P++ L I V +S  SV  +H+    +S+     +D+   A SF+ K VR + G+++  A G   ++ + D+++    Y   KP+  APP+ LYL  + Y N    +D+N W D    N T
Sbjct:   10 LNISSRTDKDVHALMNTVSVRHSGPIDYSVALLRLMLNASLKQSGESIRIHLIRPVPLSSGEFSLVTGVARREYVYRLALLAEPGLTLMHPEAAWLGSSRWLVEPPLSLELLQQAAGVLNEEHDFSAFTNLNGLSRCYTPLRRLEICVDDSV-SVDRLHACNSFTSDCFTGCVDLHFGARSFLYKQVRFMAGALVATAKGTVTLDQLKDMLSNPSDYTGLKPYDAAPPHGLYLKCVRYANP--KWDLN-WPDIQADNAT 264
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A1I8HQ33 (tRNA pseudouridine synthase OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 4.926e-31
Identity = 91/315 (28.89%), Postives = 151/315 (47.94%), Query Frame = 2 
Query:    5 SQKCRNLKQFMLHISYVGTQYQGISKSSK----RAVISVIEEQLLKLLPENKPFG-LKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPL---DMNIISNVFRRTYLYRIGLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNIT 922
            S K     Q +L++SY G  Y G +  S     R+V   +   L++L+  ++    L  SSRTD+ VHA  NTV    +    ++   LR  +N +L ++ + IRIH ++ +PL   + ++++ V RR Y+YR+  ++     L +   AW       V  P + EL  + A  L+G +++  FTNL G+     P++ L I V +S  SV  +H+    +S       D F     FM        G+++  A G   ++ + D+++    Y   KP+  APP+ LYL  + Y N    +D+N W D    N T
Sbjct:   43 SDKTAGRSQLLLYVSYNGCPYNGAANHSSDWQVRSVSKALHHHLIQLMGPSRWINRLSISSRTDKDVHALMNTVSVRHSGPIDYSVALLRLMLNASLKQSGQSIRIHLIRPVPLSSGEFSLVTGVARREYVYRLAQLAEPGLTLMHPEAAWLGSSRWLVEPPLSLELLHQAAGVLNGEHDFSAFTNLNGLSRCYTPLRRLEICVDDSV-SVDRLHACNSFTS-------DCFTGCVRFM-------AGALVATAKGTVTLDQLKDMLSNPPDYTGLKPYDAAPPHGLYLKCVCYANP--KWDLN-WPDIQADNAT 339
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A1I8HPP9 (tRNA pseudouridine synthase OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 8.444e-28
Identity = 73/245 (29.80%), Postives = 125/245 (51.02%), Query Frame = 2 
Query:  200 FNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPL---DMNIISNVFRRTYLYRIGLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNIT 922
             NTV    +    ++   LR  +N +L ++ E IRIH ++ +PL   + ++++ V RR Y+YR+ L++     L +   AW       V  P + EL  + A  L+G +++  FTNL G+     P++ L I V +S  SV  +H+    +S+     +D+   A SF+ K VR + G+++  A G   ++ + D+++    Y   KP+  APP+ LYL  + Y N    +D+N W D    N T
Sbjct:    1 MNTVSVRHSGPIDYSVALLRLMLNASLKQSGESIRIHLIRPVPLSSGEFSLVTGVARREYVYRLALLAEPGLTLMHPEAAWLGSSRWLVEPPLSLELLHQAAGVLNGEHDFSAFTNLNGLSRCYTPLRRLEICVDDSV-SVDRLHACNSFTSDCFTGCVDLHFGARSFLYKQVRFMAGALVATAKGTVTLDQLKDMLSNPPDYTGLKPYDAAPPHGLYLKCVRYANP--KWDLN-WPDIRADNAT 241
[Close]
BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Match: A0A3B3DSG8 (tRNA pseudouridine synthase OS=Oryzias melastigma OX=30732 PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.845e-27
Identity = 87/302 (28.81%), Postives = 140/302 (46.36%), Query Frame = 2 
Query:   20 NLKQFMLHISYVGTQYQGISKSS-----KRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGL---------ISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHL 874
            N  ++++   Y+GT+Y G+ K       KR V   +EE + KL P N P  L  SSRTD GVHAF N+  F     N+   F  + L   +NY+L    E IRI++   +P D +       RTY+YRI L         ++ SN  WN                 + E   E A  L GT+++  F  +   ++ K+PVK L++   +   S  NM+ H  +S      ++++  T+ SF+ K VR + G+++ V  G   +  +  ++         K  + APP+ L+L  + Y+   L
Sbjct:    3 NCARYLIFFQYIGTKYSGVVKVPPHQMVKRGVQDHLEEAIQKLRP-NNPVSLLVSSRTDTGVHAFSNSAHFDLQRKNNKPPFAEELLVEALNYHLK--PEQIRINRAHRVPCDFHARYRAQSRTYVYRIALGVRHRTLLPLTESNLCWNIQ-----------DTELDEEAMREAAAVLVGTHDFSSFRAVSSDMSLKNPVKTLDVASVQMGNSFANMYFHRQIS------FLELTFTSRSFLYKQVRRMTGALVAVGRGLLTVSQLRALLEAKDSLAYPKNLV-APPHGLFLTRVAYRESDL 283
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:103044309])

HSP 1 Score: 82.0333 bits (201), Expect = 4.724e-17
Identity = 78/298 (26.17%), Postives = 130/298 (43.62%), Query Frame = 2 
Query:   20 NLKQFMLHISYVGTQYQGISK----SSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRI--GLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNC---RMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHL 874
            N  +++L   Y+GT+Y G+ K         V + IE  + +L P N+   L  SSRTD GVHA  N+        +    F  K L   +N  L +  E IRI +   +  + +   +   RTY+YR+  GL S S+  +      W  +  K      +     E +  L+GT+++  F       + K+PVK + +   +   S    H       N  ++Y ++     S++ K VR + G+++ V  G     +M+ + D  +   Y  N    +CAP   LYL  +EY +  L
Sbjct:    4 NCTRYLLFFQYLGTKYSGVMKIPAHQPVEGVQNHIENAVRRLKPLNE-VSLYISSRTDTGVHALCNSAHLDIQRKDDKPPFTGKVLTDALNLYLKK--EPIRITRAYRVSNEFHARHSAISRTYVYRLATGLSSYSDMPITEKDLCWALRDTK-----LDISAMEEASAVLTGTHDFSTFQARGS--DPKNPVKTMEMVQVQPGLSFSQRH------FNRDIEYWELSFKCRSYLYKQVRRMTGALVAVGQGKLSVRQMQKLLDARDSLAYPQN----MCAPACGLYLTKVEYNDSDL 281
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:103044309])

HSP 1 Score: 71.2478 bits (173), Expect = 1.515e-13
Identity = 69/264 (26.14%), Postives = 114/264 (43.18%), Query Frame = 2 
Query:  110 IEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRI--GLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNC---RMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHL 874
            IE  + +L P N+   L  SSRTD GVHA  N+        +    F  K L   +N  L +  E IRI +   +  + +   +   RTY+YR+  GL S S+  +      W  +  K      +     E +  L+GT+++  F       + K+PVK + +   +   S    H       N  ++Y ++     S++ K VR + G+++ V  G     +M+ + D  +   Y  N    +CAP   LYL  +EY +  L
Sbjct:   16 IENAVRRLKPLNE-VSLYISSRTDTGVHALCNSAHLDIQRKDDKPPFTGKVLTDALNLYLKK--EPIRITRAYRVSNEFHARHSAISRTYVYRLATGLSSYSDMPITEKDLCWALRDTK-----LDISAMEEASAVLTGTHDFSTFQARGS--DPKNPVKTMEMVQVQPGLSFSQRH------FNRDIEYWELSFKCRSYLYKQVRRMTGALVAVGQGKLSVRQMQKLLDARDSLAYPQN----MCAPACGLYLTKVEYNDSDL 259
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:103044309])

HSP 1 Score: 69.707 bits (169), Expect = 8.906e-13
Identity = 75/297 (25.25%), Postives = 125/297 (42.09%), Query Frame = 2 
Query:   20 NLKQFMLHISYVGTQYQGISK----SSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRI--GLISNSNF-LWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRN--LVGSMIDVAIGNC---RMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKN 865
            N  +++L   Y+GT+Y G+ K         V + IE  + +L P N+   L  SSRTD GVHA  N+        +    F  K L   +N  L +  E IRI +   +  + +   +   RTY+YR+  GL S S+  +      W  +  K      +     E +  L+GT+++  F       + K+PVK + +   +   S    H +         +Y  V +     M    R+  + G+++ V  G     +M+ + D  +   Y  N    +CAP   LYL  +EY +
Sbjct:    4 NCTRYLLFFQYLGTKYSGVMKIPAHQPVEGVQNHIENAVRRLKPLNE-VSLYISSRTDTGVHALCNSAHLDIQRKDDKPPFTGKVLTDALNLYLKK--EPIRITRAYRVSNEFHARHSAISRTYVYRLATGLSSYSDMPITEKDLCWALRDTK-----LDISAMEEASAVLTGTHDFSTFQARGS--DPKNPVKTMEMVQVQPGLSFSQRHFN---------RYTSVLIAHHYEMQIWYRSYLMTGALVAVGQGKLSVRQMQKLLDARDSLAYPQN----MCAPACGLYLTKVEYND 277
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005107.1 (pep scaffold:Pmarinus_7.0:GL480125:12705:18545:-1 gene:ENSPMAG00000004636.1 transcript:ENSPMAT00000005107.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 2.290e-7
Identity = 63/268 (23.51%), Postives = 112/268 (41.79%), Query Frame = 2 
Query:   26 KQFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLL------PEN-----KPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLS-------GTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVA---IGNCRMEDV 766
            K+  L ++Y G  Y G+ +++  A    IE++L++ L      P+N     +    +  +RTD+GV A    V     S   +   D+   +N +L    + IRI  ++ +    N       RTY+Y   L+ +  F      AWE           +F L  ET   ++       GT+N+  FT+ +G   A++      +T     P  L             +++  + +  +SFM   +R +VG ++ VA    G+ RM+D 
Sbjct:   12 KKVALLVAYSGKGYHGMQRNAGNAAYRTIEDELVEALVRAGCIPDNHAADMRKMSFQRCARTDKGVSAIGQVV-----SLKMWLIDDVVNKINAHLP---QQIRIMGMQRVTNGFNSKQLCDARTYIY---LLPSFTFGPKGQAAWEE----------SFRLPEETLSHVNALLRRYVGTHNFHNFTSGKG---AQEASARRYVTEAYCQPPSLQQ----------GIEFALIRIRGQSFMLHQIRKMVGLVVAVARGHAGDQRMQDA 245
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:101163566])

HSP 1 Score: 104.375 bits (259), Expect = 9.507e-25
Identity = 81/302 (26.82%), Postives = 134/302 (44.37%), Query Frame = 2 
Query:   20 NLKQFMLHISYVGTQYQGISKSS-----KRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGL---------ISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHL 874
            N  ++++   Y+GT+Y G+ K       K+ V   IEE + KL P N    L  SSRTD GVHA  N+  F     N+   F    L   +NY+L   +E IRI+Q   +  D +       RTY+YR+ L         ++ SN  W+                 + E   E A  L GT+++  F  +   +  K+PVK L++   +   S  + + H        V ++++  T+ SF+ K VR + G+++ V  G   +  + D++      L     + AP + L+L  + Y+   L
Sbjct:    3 NGARYLIFFQYIGTKYSGVVKVPPQQMVKKGVQDHIEEAIQKLKPVNSA-SLSVSSRTDTGVHALSNSAHFDLQRRNNKPPFAEDLLVEALNYHLQ--TEQIRINQAHRVADDFHARYRAQSRTYVYRVALGVRHRTLLPLTESNLCWDIQ-----------DTELDVEAMREAAAVLIGTHDFSSFRAVNSDMTFKNPVKTLDVASIQLGNSFASTYFHR------QVSFLELTFTSRSFLYKQVRRMTGALVAVGRGLLTVSQLRDLLE-AKDNLAYPQNLVAPAHGLFLTQVAYRESDL 283
[Close]
BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Match: pusl1 (pseudouridine synthase like 1 [Source:NCBI gene;Acc:101163566])

HSP 1 Score: 90.5077 bits (223), Expect = 6.319e-20
Identity = 71/267 (26.59%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  110 IEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRF---LTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGL---------ISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHL 874
            I+E + KL P N    L  SSRTD GVHA  N+  F     N+   F    L   +NY+L   +E IRI+Q   +  D +       RTY+YR+ L         ++ SN  W+                 + E   E A  L GT+++  F  +   +  K+PVK L++   +   S  + + H        V ++++  T+ SF+ K VR + G+++ V  G   +  + D++      L     + AP + L+L  + Y+   L
Sbjct:   39 IQEAIQKLKPVNSA-SLSVSSRTDTGVHALSNSAHFDLQRRNNKPPFAEDLLVEALNYHLQ--TEQIRINQAHRVADDFHARYRAQSRTYVYRVALGVRHRTLLPLTESNLCWDIQ-----------DTELDVEAMREAAAVLIGTHDFSSFRAVNSDMTFKNPVKTLDVASIQLGNSFASTYFHR------QVSFLELTFTSRSFLYKQVRRMTGALVAVGRGLLTVSQLRDLLE-AKDNLAYPQNLVAPAHGLFLTQVAYRESDL 284
[Close]
BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Match: SMESG000053891.1 (SMESG000053891.1)

HSP 1 Score: 678.707 bits (1750), Expect = 0.000e+0
Identity = 325/326 (99.69%), Postives = 326/326 (100.00%), Query Frame = 2 
Query:    2 MSQKCRNLKQFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNITDFEPKIEVPIIPKDEKLEI 979
            MSQKCRNLKQFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCR+EDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNITDFEPKIEVPIIPKDEKLEI
Sbjct:    1 MSQKCRNLKQFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRLEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNITDFEPKIEVPIIPKDEKLEI 326
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BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Match: SMESG000053891.1 (SMESG000053891.1)

HSP 1 Score: 654.825 bits (1688), Expect = 0.000e+0
Identity = 314/315 (99.68%), Postives = 315/315 (100.00%), Query Frame = 2 
Query:   35 MLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNITDFEPKIEVPIIPKDEKLEI 979
            MLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCR+EDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNITDFEPKIEVPIIPKDEKLEI
Sbjct:    1 MLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKPFGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMIRIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKFVGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESAPSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNCRLEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQDESRYNITDFEPKIEVPIIPKDEKLEI 315
The following BLAST results are available for this feature:
BLAST of tRNA pseudouridine synthase A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PUSL14.525e-1021.21pseudouridine synthase like 1 [Source:HGNC Symbol;... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG341401.625e-1123.17gene:FBgn0083976 transcript:FBtr0110975[more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
pusl15.065e-1623.03pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
pusl18.712e-1623.21pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
pusl11.806e-821.84pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
PUSL13.681e-1423.53pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
PUSL12.407e-1323.67pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pusl12.730e-821.28pseudouridylate synthase-like 1 [Source:MGI Symbol... [more]
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BLAST of tRNA pseudouridine synthase A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A9ILJ7|TRUA_BART13.111e-1824.03tRNA pseudouridine synthase A OS=Bartonella triboc... [more]
sp|B8E1G5|TRUA_DICTD5.050e-1724.82tRNA pseudouridine synthase A OS=Dictyoglomus turg... [more]
sp|Q6G547|TRUA_BARHE7.392e-1724.11tRNA pseudouridine synthase A OS=Bartonella hensel... [more]
sp|B4U9D9|TRUA_HYDS02.570e-1627.34tRNA pseudouridine synthase A OS=Hydrogenobaculum ... [more]
sp|Q92SH4|TRUA_RHIME6.436e-1625.53tRNA pseudouridine synthase A OS=Rhizobium melilot... [more]
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BLAST of tRNA pseudouridine synthase A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HE351.851e-3830.79tRNA pseudouridine synthase OS=Macrostomum lignano... [more]
A0A267FZ266.181e-3331.27tRNA pseudouridine synthase OS=Macrostomum lignano... [more]
A0A1I8HQ334.926e-3128.89tRNA pseudouridine synthase OS=Macrostomum lignano... [more]
A0A1I8HPP98.444e-2829.80tRNA pseudouridine synthase OS=Macrostomum lignano... [more]
A0A3B3DSG81.845e-2728.81tRNA pseudouridine synthase OS=Oryzias melastigma ... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
pusl14.724e-1726.17pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
pusl11.515e-1326.14pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
pusl18.906e-1325.25pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005107.12.290e-723.51pep scaffold:Pmarinus_7.0:GL480125:12705:18545:-1 ... [more]
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA pseudouridine synthase A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pusl19.507e-2526.82pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
pusl16.319e-2026.59pseudouridine synthase like 1 [Source:NCBI gene;Ac... [more]
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BLAST of tRNA pseudouridine synthase A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000053891.10.000e+099.69SMESG000053891.1[more]
SMESG000053891.10.000e+099.68SMESG000053891.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018566 ID=SMED30018566|Name=tRNA pseudouridine synthase A|organism=Schmidtea mediterranea sexual|type=transcript|length=1353bp
CATGTCGCAGAAATGTCGCAATCTAAAACAATTTATGTTACATATATCTT
ATGTTGGTACTCAATACCAAGGTATTTCAAAGAGTTCTAAAAGAGCGGTG
ATTTCTGTAATTGAAGAACAGTTATTAAAACTTTTACCAGAAAATAAGCC
CTTTGGATTAAAAACATCAAGTAGAACTGACCAAGGAGTTCATGCATTTT
TTAATACTGTCCGATTTCTTACGAATTCGCATTATTCATTTAATTCAAAA
GATTTAAGGTATTATATGAATTATAATCTATCAAGAGCATCTGAAATGAT
AAGAATACACCAAGTAAAAGAAATTCCACTTGATATGAACATAATATCCA
ATGTTTTTCGTCGAACTTATTTATACAGAATTGGTTTGATTTCAAATTCT
AATTTTCTTTGGAATTGCGCAACTGCATGGGAAAGTCAGCAAATTAAATT
TGTAGGGGCTCCATTCAATTTTGAATTATTTCTCGAGACAGCCAAGTGTT
TATCCGGCACTTACAATTATTGCGGATTCACAAATCTAGAGGGAATGTTA
AATGCAAAAGACCCGGTGAAAAATCTAAACATTACAGTGACAGAATCGGC
TCCCTCAGTATTAAATATGCATTCCCATCCGGTGCTATCCAGTAACTTTC
CTGTGAAATATATTGACGTTTTTGTTACGGCGGAAAGTTTCATGAACAAA
ATGGTTCGCAACCTTGTTGGCAGCATGATTGATGTCGCTATAGGAAATTG
TCGAATGGAAGACGTTTCAGATGTCGTCAATAGAGGTCATTATTATTTAA
ACGAAAAACCATTTATCTGTGCTCCCCCATACGCTTTGTACTTGTACAAT
ATTGAGTACAAAAATGAACATTTGAATTTTGATGTGAACAAATGGCAAGA
TGAATCTAGATACAATATAACTGACTTTGAACCTAAGATTGAGGTGCCGA
TTATTCCCAAAGATGAAAAATTGGAAATATAATTGATTTTTTATTTATTG
ACTTTTAAAAATTTCCCTAATGTGTGTATGTGTGCTGATTTCGAAACTCC
AAGTGAGTTTGCTTTCTGGCATTATCTGGATTTATTGATGTTGTCTTGGC
AACCGTATTGTAGTTAAGTTTGCATGTAAGATCGTTGCTATAACTATTTT
CCGACTAATGATTCACTTCCAATAGGGAAATAGTTTTATTTATTCATAAA
TGAAACTTTTGGTGGGTTTCTGAGATTTGGTGGGTGTTGAGATTTTGAAG
CCATATAGTTGTTACTGTGGGGTTAGTGGTGTGGATGGCCTTCACCCCAA
TACATACAAATTAATTTGACGCCAGCCAATGGTTACCCGTTGCTAGGAAA
CGT
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protein sequence of SMED30018566-orf-1

>SMED30018566-orf-1 ID=SMED30018566-orf-1|Name=SMED30018566-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=327bp
MSQKCRNLKQFMLHISYVGTQYQGISKSSKRAVISVIEEQLLKLLPENKP
FGLKTSSRTDQGVHAFFNTVRFLTNSHYSFNSKDLRYYMNYNLSRASEMI
RIHQVKEIPLDMNIISNVFRRTYLYRIGLISNSNFLWNCATAWESQQIKF
VGAPFNFELFLETAKCLSGTYNYCGFTNLEGMLNAKDPVKNLNITVTESA
PSVLNMHSHPVLSSNFPVKYIDVFVTAESFMNKMVRNLVGSMIDVAIGNC
RMEDVSDVVNRGHYYLNEKPFICAPPYALYLYNIEYKNEHLNFDVNKWQD
ESRYNITDFEPKIEVPIIPKDEKLEI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0031119tRNA pseudouridine synthesis
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
GO:0008033tRNA processing
Vocabulary: INTERPRO
TermDefinition
IPR001406PsdUridine_synth_TruA
IPR020095PsdUridine_synth_TruA_C
IPR020094PsdUridine_synth_TruA_N
IPR020097PsdUridine_synth_TruA_a/b_dom
IPR020103PsdUridine_synth_cat_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO:0016853isomerase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3DG3DSA:3.30.70.660coord: 119..289
e-value: 3.2E-15
score: 58.1
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 182..286
e-value: 1.3E-8
score: 35.2
NoneNo IPR availableGENE3DG3DSA:3.30.70.580coord: 8..116
e-value: 9.5E-14
score: 53.6
NoneNo IPR availablePANTHERPTHR11142:SF0TRNA PSEUDOURIDINE SYNTHASEcoord: 6..299
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 6..299
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILYSSF55120Pseudouridine synthasecoord: 9..294