Pre-mRNA-splicing regulator female-lethal(2)D

Overview
NamePre-mRNA-splicing regulator female-lethal(2)D
Smed IDSMED30018505
Length (bp)924
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pre-mRNA-splicing regulator female-lethal(2)D (SMED30018505) t-SNE clustered cells

Violin plots show distribution of expression levels for Pre-mRNA-splicing regulator female-lethal(2)D (SMED30018505) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pre-mRNA-splicing regulator female-lethal(2)D (SMED30018505) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pre-mRNA-splicing regulator female-lethal(2)D (SMED30018505) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018505

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30018505SMESG000043668.1 SmedASXL_000866SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30018505SMESG000043668.1 SmedASXL_000866SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Human
Match: WTAP (WT1 associated protein [Source:HGNC Symbol;Acc:HGNC:16846])

HSP 1 Score: 154.836 bits (390), Expect = 1.696e-43
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Human
Match: WTAP (WT1 associated protein [Source:HGNC Symbol;Acc:HGNC:16846])

HSP 1 Score: 154.836 bits (390), Expect = 1.696e-43
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Human
Match: WTAP (WT1 associated protein [Source:HGNC Symbol;Acc:HGNC:16846])

HSP 1 Score: 83.1889 bits (204), Expect = 5.480e-19
Identity = 39/91 (42.86%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPD 333
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPD
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPD 150
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Human
Match: WTAP (WT1 associated protein [Source:HGNC Symbol;Acc:HGNC:16846])

HSP 1 Score: 83.1889 bits (204), Expect = 5.480e-19
Identity = 39/91 (42.86%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPD 333
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPD
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPD 150
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Human
Match: WTAP (WT1 associated protein [Source:HGNC Symbol;Acc:HGNC:16846])

HSP 1 Score: 83.9593 bits (206), Expect = 5.982e-19
Identity = 39/91 (42.86%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPD 333
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPD
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPD 150
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Fly
Match: fl(2)d (gene:FBgn0000662 transcript:FBtr0304697)

HSP 1 Score: 172.555 bits (436), Expect = 2.181e-50
Identity = 84/172 (48.84%), Postives = 121/172 (70.35%), Query Frame = 1 
Query:   94 KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:   10 RREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 181
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Fly
Match: fl(2)d (gene:FBgn0000662 transcript:FBtr0087648)

HSP 1 Score: 172.555 bits (436), Expect = 2.181e-50
Identity = 84/172 (48.84%), Postives = 121/172 (70.35%), Query Frame = 1 
Query:   94 KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:   10 RREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 181
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Fly
Match: fl(2)d (gene:FBgn0000662 transcript:FBtr0304696)

HSP 1 Score: 172.555 bits (436), Expect = 2.181e-50
Identity = 84/172 (48.84%), Postives = 121/172 (70.35%), Query Frame = 1 
Query:   94 KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:   10 RREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 181
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Fly
Match: fl(2)d (gene:FBgn0000662 transcript:FBtr0087647)

HSP 1 Score: 172.555 bits (436), Expect = 2.018e-49
Identity = 91/191 (47.64%), Postives = 131/191 (68.59%), Query Frame = 1 
Query:   43 ENNKLKEQIEIKNRELD--KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            EN KL EQ+ +  +  D  + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:  116 ENLKL-EQVRMAQQCADAQRREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 305
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Zebrafish
Match: wtap (WT1 associated protein [Source:NCBI gene;Acc:334058])

HSP 1 Score: 157.147 bits (396), Expect = 2.741e-44
Identity = 76/180 (42.22%), Postives = 125/180 (69.44%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISEL 600
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL   +  +S++
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSAAQLRSSMVDPAINLFFLKMKAELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLRETRQQLSQM 239
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Zebrafish
Match: wtap (WT1 associated protein [Source:NCBI gene;Acc:334058])

HSP 1 Score: 83.9593 bits (206), Expect = 2.151e-19
Identity = 39/91 (42.86%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPD 333
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPD
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSAAQLRSSMVDPAINLFFLKMKAELEQTKDKLEQAQNELSAWKFTPD 150
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Xenopus
Match: wtap (WT1 associated protein [Source:NCBI gene;Acc:394807])

HSP 1 Score: 153.295 bits (386), Expect = 5.150e-43
Identity = 77/173 (44.51%), Postives = 119/173 (68.79%), Query Frame = 1 
Query:   52 KLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            KLK+Q +   R     E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ F+++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   61 KLKQQQQDSARR----ENILVMRLATKEQEMQECTTQIQHLKQVQQPSVAQLRATMVDPAINLFFIKMKAELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Mouse
Match: Wtap (Wilms tumour 1-associating protein [Source:MGI Symbol;Acc:MGI:1926395])

HSP 1 Score: 154.836 bits (390), Expect = 1.307e-43
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Mouse
Match: Wtap (Wilms tumour 1-associating protein [Source:MGI Symbol;Acc:MGI:1926395])

HSP 1 Score: 154.836 bits (390), Expect = 1.307e-43
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Mouse
Match: Wtap (Wilms tumour 1-associating protein [Source:MGI Symbol;Acc:MGI:1926395])

HSP 1 Score: 85.1149 bits (209), Expect = 6.314e-20
Identity = 43/95 (45.26%), Postives = 64/95 (67.37%), Query Frame = 1 
Query:   52 KLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDS 336
            KLK+Q     +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDS
Sbjct:   38 KLKQQ----QQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDS 128
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Mouse
Match: Wtap (Wilms tumour 1-associating protein [Source:MGI Symbol;Acc:MGI:1926395])

HSP 1 Score: 83.1889 bits (204), Expect = 3.762e-19
Identity = 39/91 (42.86%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPD 333
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPD
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPD 150
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Mouse
Match: Wtap (Wilms tumour 1-associating protein [Source:MGI Symbol;Acc:MGI:1926395])

HSP 1 Score: 83.1889 bits (204), Expect = 3.762e-19
Identity = 39/91 (42.86%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPD 333
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPD
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPD 150
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. UniProt/SwissProt
Match: sp|Q9Y091|FL2D_DROME (Pre-mRNA-splicing regulator female-lethal(2)D OS=Drosophila melanogaster OX=7227 GN=fl(2)d PE=1 SV=2)

HSP 1 Score: 172.555 bits (436), Expect = 2.023e-48
Identity = 91/191 (47.64%), Postives = 131/191 (68.59%), Query Frame = 1 
Query:   43 ENNKLKEQIEIKNRELD--KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            EN KL EQ+ +  +  D  + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:  116 ENLKL-EQVRMAQQCADAQRREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 305
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. UniProt/SwissProt
Match: sp|Q28XY0|FL2D_DROPS (Pre-mRNA-splicing regulator female-lethal(2)D OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=fl(2)d PE=3 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 7.306e-48
Identity = 83/172 (48.26%), Postives = 121/172 (70.35%), Query Frame = 1 
Query:   94 KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L ++
Sbjct:  141 RREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKTKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKD 312
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. UniProt/SwissProt
Match: sp|Q7SXL7|FL2D_DANRE (Pre-mRNA-splicing regulator WTAP OS=Danio rerio OX=7955 GN=wtap PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 2.069e-43
Identity = 76/180 (42.22%), Postives = 125/180 (69.44%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISEL 600
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL   +  +S++
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSAAQLRSSMVDPAINLFFLKMKAELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLRETRQQLSQM 239
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. UniProt/SwissProt
Match: sp|Q15007|FL2D_HUMAN (Pre-mRNA-splicing regulator WTAP OS=Homo sapiens OX=9606 GN=WTAP PE=1 SV=2)

HSP 1 Score: 154.836 bits (390), Expect = 8.145e-43
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. UniProt/SwissProt
Match: sp|Q9ER69|FL2D_MOUSE (Pre-mRNA-splicing regulator WTAP OS=Mus musculus OX=10090 GN=Wtap PE=1 SV=3)

HSP 1 Score: 154.836 bits (390), Expect = 9.046e-43
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. TrEMBL
Match: A0A1S3K209 (pre-mRNA-splicing regulator WTAP OS=Lingula unguis OX=7574 GN=LOC106178079 PE=4 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 6.132e-51
Identity = 97/186 (52.15%), Postives = 133/186 (71.51%), Query Frame = 1 
Query:   52 KLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            KLK+Q      E  + E+VL+  L  KEQE+ D   QI+++K    P + QLR M LDPAVN++F R+KK+M ++ EKLEQTQN+L AWKFTPDSQTGKR+M + R+LLQENEELGK+  SGRTAKLE EI++++ L+EELK + +E E  + E+D + E  Q++I++ QQQL  A+  I +L  E
Sbjct:   62 KLKQQ----QLEATRRENVLVMRLTTKEQELQDYITQIQEIKQAQVPSSAQLRNMLLDPAVNLMFQRMKKEMDSSKEKLEQTQNELSAWKFTPDSQTGKRLMAKCRMLLQENEELGKVISSGRTAKLEGEIALEKKLVEELKNSQAETEEFLVEIDEDVEAMQSTIYLYQQQLKEAQEQIIKLQEE 243
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. TrEMBL
Match: R7UYR9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_221587 PE=4 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 1.518e-47
Identity = 90/181 (49.72%), Postives = 128/181 (70.72%), Query Frame = 1 
Query:   52 KLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTIS 594
            KLK+Q E    E  + E +L+  L  KEQE+    AQI +MK   N    QLR M LDPA+N++F R+KK+M+ A E+ EQ +ND+ AWKFTPDS TGKR+M + R+LLQENEE+GK+  SGRTAKLE EI++++  IEE+K++ +E ++ + EMD + E  Q++I++LQQQL  A+  +S
Sbjct:   54 KLKQQQE----EATRRESILVLRLTTKEQEVQKALAQINEMKQAENSSAAQLRSMLLDPAINLMFQRMKKEMETAKERQEQAENDMAAWKFTPDSHTGKRLMNKCRMLLQENEEIGKVIASGRTAKLEGEIALEKKFIEEMKKSQAETDDFLVEMDEDVEGMQSTIYLLQQQLKDARDQLS 230
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. TrEMBL
Match: B3MHE2 (Uncharacterized protein OS=Drosophila ananassae OX=7217 GN=Dana\GF11164 PE=4 SV=2)

HSP 1 Score: 173.326 bits (438), Expect = 2.577e-47
Identity = 84/172 (48.84%), Postives = 121/172 (70.35%), Query Frame = 1 
Query:   94 KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:   10 RREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKSKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 181
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. TrEMBL
Match: A0A1W4VGK5 (pre-mRNA-splicing regulator female-lethal(2)D isoform X2 OS=Drosophila ficusphila OX=30025 GN=LOC108094873 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 4.692e-47
Identity = 84/172 (48.84%), Postives = 121/172 (70.35%), Query Frame = 1 
Query:   94 KLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F R+KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E++M+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:   10 RREKILMRRLANKEQEFQDYVSQIAEYKAQQAPTALALRTALLDPAVNLLFERLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELAMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQTLEKE 181
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. TrEMBL
Match: A0A1I8MDP2 (Uncharacterized protein OS=Musca domestica OX=7370 GN=101893404 PE=4 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 5.218e-47
Identity = 89/190 (46.84%), Postives = 132/190 (69.47%), Query Frame = 1 
Query:   43 ENNKLKEQ-IEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRE 609
            EN KL++Q + ++  +  + E +L+  LA+KEQE  D  +QI + KA   P    LR   LDPAVN++F ++KK++KA   KLE+TQN+L AWKFTPDS TGKR+M + R+L QENEELGKM  +GR AKLE+E+SM+++  EE+K++ SE ++ ++E+D + E  Q++I  LQQ+L   +  I  L +E
Sbjct:   10 ENLKLEQQRLTMQCTDAQRREKILMRRLANKEQEFQDYVSQIAEYKAAQAPSAVALRSALLDPAVNLLFQKLKKELKATKAKLEETQNELSAWKFTPDSNTGKRLMAKCRLLYQENEELGKMTSNGRLAKLETELSMQKSFSEEVKKSQSELDDFLQELDEDVEGMQSTILFLQQELKTTRDRIQVLEKE 199
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Cavefish
Match: wtap (WT1 associated protein [Source:NCBI gene;Acc:103037461])

HSP 1 Score: 155.606 bits (392), Expect = 8.231e-44
Identity = 75/170 (44.12%), Postives = 120/170 (70.59%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSAAQLRSSMVDPAINLFFLKMKAELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQL 229
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Sea Lamprey
Match: wtap (WT1 associated protein [Source:NCBI gene;Acc:334058])

HSP 1 Score: 139.043 bits (349), Expect = 7.020e-39
Identity = 74/197 (37.56%), Postives = 127/197 (64.47%), Query Frame = 1 
Query:    1 MNSSLEDQNSVLFKENNKLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTI 591
            + S L D NS       + +E+++ + +E  + E++L+  LA KEQE+ + + QI  +K         LR   +DPAVN++F ++K +++ + +KL+Q  ++L AWKFTPDSQTGK++M++ R+L+QEN+ELG+    GR A+LE+E+++++   +ELK    E  + I ++D E E  Q++I +LQQQL  A+  +
Sbjct:   40 LESKLADLNSSDVTGLRESEERLKHQQQESLRRENILVMRLATKEQEMQECATQIASLKQGQLSSASHLRSCLVDPAVNLLFTKMKSELQQSKDKLQQAHDELSAWKFTPDSQTGKKLMSKCRMLIQENQELGRQLSQGRVAQLEAELALQKKYSQELKSGQDELNDFIIQLDEEVEGMQSTILVLQQQLKEARHKL 236
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Nematostella
Match: EDO46685 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPH4])

HSP 1 Score: 154.836 bits (390), Expect = 3.671e-44
Identity = 80/173 (46.24%), Postives = 116/173 (67.05%), Query Frame = 1 
Query:   52 KLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQL 570
            KLK+Q     +E  + E+VL+  LA KE E+ DL  QI D+K   NP   Q+R   LDPAVN++F R+K ++  A  KLEQ QNDL AWKFTPDS TGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   +ELK +  E  + + ++D E E  Q++I  LQQQ+
Sbjct:   76 KLKQQ----QQESTRRENVLVMRLATKEHEVQDLLTQIHDLKQAQNPSTVQMRSTLLDPAVNLLFQRMKTELDEAKVKLEQAQNDLSAWKFTPDSVTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSDELKSSQDELNDFVIQLDEEVEGMQSTIMTLQQQI 244
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Medaka
Match: wtap (WT1 associated protein [Source:NCBI gene;Acc:101154997])

HSP 1 Score: 157.147 bits (396), Expect = 1.763e-44
Identity = 77/179 (43.02%), Postives = 125/179 (69.83%), Query Frame = 1 
Query:   61 EQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISE 597
            E+++ + +E  + E++L+  LA KEQE+ + + QI+ +K V  P   QLR   +DPA+N+ FL++K +++   +KLEQ QN+L AWKFTPDSQTGK++M + R+L+QEN+ELG+    GR A+LE+E+++++   EELK +  E  + I ++D E E  Q++I +LQQQL  ++  IS+
Sbjct:   60 EKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSAAQLRSSMVDPAINLFFLKMKAELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKESRQLISQ 238
[Close]
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Planmine SMEST
Match: SMESG000043668.1 (SMESG000043668.1)

HSP 1 Score: 601.282 bits (1549), Expect = 0.000e+0
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 1 
Query:    1 MNSSLEDQNSVLFKENNKLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRELDKHSPGRSQIILTDCNWTDSGKLEPMEGVEEESSVLADVSGNVNSSNQDVNGAAGVXXXXXXXXXXXXDDTTNALTQQLILNSDCNIKTESSVFSPNEQQLHCD 924
            MNSSLEDQNSVLFKENNKLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRELDKHSPGRSQIILTDCNWTDSGKLEPMEGVEEESSVLADVSGNVNSSNQDVNGAAGVSSSSCLSNHHLSDDTTNALTQQLILNSDCNIKTESSVFSPNEQQLHCD
Sbjct:    1 MNSSLEDQNSVLFKENNKLKEQIEIKNRELDKLEHVLLANLAHKEQEITDLSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQNDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISMKRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISELSRELDKHSPGRSQIILTDCNWTDSGKLEPMEGVEEESSVLADVSGNVNSSNQDVNGAAGVSSSSCLSNHHLSDDTTNALTQQLILNSDCNIKTESSVFSPNEQQLHCD 308
The following BLAST results are available for this feature:
BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
WTAP1.696e-4344.12WT1 associated protein [Source:HGNC Symbol;Acc:HGN... [more]
WTAP1.696e-4344.12WT1 associated protein [Source:HGNC Symbol;Acc:HGN... [more]
WTAP5.480e-1942.86WT1 associated protein [Source:HGNC Symbol;Acc:HGN... [more]
WTAP5.480e-1942.86WT1 associated protein [Source:HGNC Symbol;Acc:HGN... [more]
WTAP5.982e-1942.86WT1 associated protein [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
fl(2)d2.181e-5048.84gene:FBgn0000662 transcript:FBtr0304697[more]
fl(2)d2.181e-5048.84gene:FBgn0000662 transcript:FBtr0087648[more]
fl(2)d2.181e-5048.84gene:FBgn0000662 transcript:FBtr0304696[more]
fl(2)d2.018e-4947.64gene:FBgn0000662 transcript:FBtr0087647[more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
wtap2.741e-4442.22WT1 associated protein [Source:NCBI gene;Acc:33405... [more]
wtap2.151e-1942.86WT1 associated protein [Source:NCBI gene;Acc:33405... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
wtap5.150e-4344.51WT1 associated protein [Source:NCBI gene;Acc:39480... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Wtap1.307e-4344.12Wilms tumour 1-associating protein [Source:MGI Sym... [more]
Wtap1.307e-4344.12Wilms tumour 1-associating protein [Source:MGI Sym... [more]
Wtap6.314e-2045.26Wilms tumour 1-associating protein [Source:MGI Sym... [more]
Wtap3.762e-1942.86Wilms tumour 1-associating protein [Source:MGI Sym... [more]
Wtap3.762e-1942.86Wilms tumour 1-associating protein [Source:MGI Sym... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9Y091|FL2D_DROME2.023e-4847.64Pre-mRNA-splicing regulator female-lethal(2)D OS=D... [more]
sp|Q28XY0|FL2D_DROPS7.306e-4848.26Pre-mRNA-splicing regulator female-lethal(2)D OS=D... [more]
sp|Q7SXL7|FL2D_DANRE2.069e-4342.22Pre-mRNA-splicing regulator WTAP OS=Danio rerio OX... [more]
sp|Q15007|FL2D_HUMAN8.145e-4344.12Pre-mRNA-splicing regulator WTAP OS=Homo sapiens O... [more]
sp|Q9ER69|FL2D_MOUSE9.046e-4344.12Pre-mRNA-splicing regulator WTAP OS=Mus musculus O... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3K2096.132e-5152.15pre-mRNA-splicing regulator WTAP OS=Lingula unguis... [more]
R7UYR91.518e-4749.72Uncharacterized protein OS=Capitella teleta OX=283... [more]
B3MHE22.577e-4748.84Uncharacterized protein OS=Drosophila ananassae OX... [more]
A0A1W4VGK54.692e-4748.84pre-mRNA-splicing regulator female-lethal(2)D isof... [more]
A0A1I8MDP25.218e-4746.84Uncharacterized protein OS=Musca domestica OX=7370... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
wtap8.231e-4444.12WT1 associated protein [Source:NCBI gene;Acc:10303... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
wtap7.020e-3937.56WT1 associated protein [Source:NCBI gene;Acc:33405... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO466853.671e-4446.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
wtap1.763e-4443.02WT1 associated protein [Source:NCBI gene;Acc:10115... [more]
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BLAST of Pre-mRNA-splicing regulator female-lethal(2)D vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000043668.10.000e+0100.00SMESG000043668.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018505 ID=SMED30018505|Name=Pre-mRNA-splicing regulator female-lethal(2)D|organism=Schmidtea mediterranea sexual|type=transcript|length=924bp
ATGAATAGTTCGTTAGAAGATCAAAATAGTGTCCTTTTTAAGGAAAACAA
TAAATTAAAAGAACAAATTGAAATCAAAAACAGAGAATTGGATAAATTGG
AACATGTTCTTTTAGCAAATCTAGCTCACAAAGAGCAAGAAATTACCGAT
TTATCAGCCCAAATTGAAGACATGAAGGCTGTGATGAATCCATGCAATCA
ACAATTACGCTGCATGGGTTTAGATCCAGCTGTGAACGTAGTATTTCTAC
GAATCAAAAAAGATATGAAAGCTGCAAATGAGAAGTTGGAACAAACTCAA
AATGATTTGCAGGCTTGGAAGTTTACTCCAGATAGTCAAACAGGTAAGCG
GATGATGACAAGAATGCGCGTATTATTGCAGGAAAATGAAGAGTTGGGTA
AAATGAATCAAAGTGGTCGAACTGCTAAATTGGAAAGTGAAATTTCAATG
AAACGAACTTTGATTGAGGAATTAAAGCAAACGAATAGCGAATATGAGAA
CGTAATTGAAGAAATGGACTGTGAAACTGAATGTTTCCAAACTAGCATAT
TCATGCTCCAGCAACAGCTGGCAATAGCGAAATCGACAATCTCTGAATTG
TCCCGGGAACTTGACAAACATTCTCCTGGTAGAAGTCAAATTATTTTAAC
TGACTGTAATTGGACAGACTCGGGGAAACTGGAACCAATGGAAGGCGTCG
AGGAGGAGTCCAGTGTTTTAGCTGACGTTAGTGGAAATGTTAACAGTAGT
AACCAAGACGTCAACGGAGCCGCCGGCGTTAGCAGCAGCAGTTGTCTTAG
TAACCACCATTTATCAGATGACACTACAAATGCCCTAACCCAACAACTCA
TTCTGAATTCAGATTGCAATATAAAAACCGAATCCTCTGTATTTTCCCCT
AACGAACAACAACTCCACTGTGAC
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protein sequence of SMED30018505-orf-1

>SMED30018505-orf-1 ID=SMED30018505-orf-1|Name=SMED30018505-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=308bp
MNSSLEDQNSVLFKENNKLKEQIEIKNRELDKLEHVLLANLAHKEQEITD
LSAQIEDMKAVMNPCNQQLRCMGLDPAVNVVFLRIKKDMKAANEKLEQTQ
NDLQAWKFTPDSQTGKRMMTRMRVLLQENEELGKMNQSGRTAKLESEISM
KRTLIEELKQTNSEYENVIEEMDCETECFQTSIFMLQQQLAIAKSTISEL
SRELDKHSPGRSQIILTDCNWTDSGKLEPMEGVEEESSVLADVSGNVNSS
NQDVNGAAGVSSSSCLSNHHLSDDTTNALTQQLILNSDCNIKTESSVFSP
NEQQLHCD
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007548sex differentiation
GO:0030154cell differentiation
GO:0048024regulation of mRNA splicing, via spliceosome
GO:0000375RNA splicing, via transesterification reactions
GO:0000381regulation of alternative mRNA splicing, via spliceosome
GO:0006397mRNA processing
GO:0007275multicellular organism development
GO:0007530sex determination
GO:0007539primary sex determination, soma
GO:0008380RNA splicing
GO:0019099female germ-line sex determination
GO:0046331lateral inhibition
GO:0048749compound eye development
GO:0070868heterochromatin organization involved in chromatin silencing
GO:0080009mRNA methylation
Vocabulary: cellular component
TermDefinition
GO:0036396MIS complex
GO:0005634nucleus
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR033757WTAP
IPR029732WTAP/Mum2
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 41..61
NoneNo IPR availableCOILSCoilCoilcoord: 2..36
NoneNo IPR availableCOILSCoilCoilcoord: 141..175
NoneNo IPR availableCOILSCoilCoilcoord: 82..109
IPR029732WTAP/Mum2 familyPFAMPF17098Wtapcoord: 30..183
e-value: 9.7E-52
score: 175.0
IPR033757Pre-mRNA-splicing regulator WTAPPANTHERPTHR15217WILMS' TUMOR 1-ASSOCIATING PROTEINcoord: 12..208