SMED30018491

Overview
NameSMED30018491
Smed IDSMED30018491
Length (bp)2164
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018491 (SMED30018491) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018491 (SMED30018491) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018491 (SMED30018491) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018491 (SMED30018491) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018491

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30018491SMESG000000384.1 SmedASXL_015251SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30018491SMESG000000384.1 SmedASXL_015251SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30018491SMESG000000384.1 SmedASXL_015250SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30018491SMESG000000384.1 SmedASXL_015250SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30018491SMESG000000384.1 dd_Smed_v6_10941_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30018491SMESG000000384.1 dd_Smed_v4_10941_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018491 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002944:28824..38700 -9908
Homology
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BLAST of SMED30018491 vs. Planmine SMEST
Match: SMESG000000384.1 (SMESG000000384.1)

HSP 1 Score: 1073.54 bits (2775), Expect = 0.000e+0
Identity = 529/530 (99.81%), Postives = 530/530 (100.00%), Query Frame = 1 
Query:  391 FTNQSGTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTGILIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNFNVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEXXXXXXXXXXXXSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR 1980
            FTNQSGTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTG+LIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNFNVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEIKTKKVIVKKGKSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR
Sbjct:  333 FTNQSGTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTGVLIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNFNVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEIKTKKVIVKKGKSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR 862

HSP 2 Score: 60.4622 bits (145), Expect = 4.374e-15
Identity = 31/40 (77.50%), Postives = 31/40 (77.50%), Query Frame = 3 
Query:  255 IKSFKLHLKAEKPQFAEIRIISPSTPFHVNIFVFSHNCNY 374
            IKSFKLHLKAEKPQFAEIRIISPSTPFH   F      NY
Sbjct:  305 IKSFKLHLKAEKPQFAEIRIISPSTPFH---FTNQSGTNY 341

HSP 3 Score: 40.4318 bits (93), Expect = 4.374e-15
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 1 
Query:  109 SKPKYEEIRAWLQHNQM 159
            SKPKYEEIRAWLQHNQ+
Sbjct:  288 SKPKYEEIRAWLQHNQI 304
[Close]
BLAST of SMED30018491 vs. Planmine SMEST
Match: SMESG000000384.1 (SMESG000000384.1)

HSP 1 Score: 1073.15 bits (2774), Expect = 0.000e+0
Identity = 529/530 (99.81%), Postives = 530/530 (100.00%), Query Frame = 1 
Query:  391 FTNQSGTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTGILIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNFNVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEXXXXXXXXXXXXSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR 1980
            FTNQSGTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTG+LIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNFNVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEIKTKKVIVKKGKSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR
Sbjct:  316 FTNQSGTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTGVLIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNFNVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEIKTKKVIVKKGKSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR 845

HSP 2 Score: 60.8474 bits (146), Expect = 3.005e-9
Identity = 31/41 (75.61%), Postives = 31/41 (75.61%), Query Frame = 3 
Query:  252 SIKSFKLHLKAEKPQFAEIRIISPSTPFHVNIFVFSHNCNY 374
             IKSFKLHLKAEKPQFAEIRIISPSTPFH   F      NY
Sbjct:  287 DIKSFKLHLKAEKPQFAEIRIISPSTPFH---FTNQSGTNY 324
[Close]
BLAST of SMED30018491 vs. Planmine SMEST
Match: SMESG000072635.1 (SMESG000072635.1)

HSP 1 Score: 116.701 bits (291), Expect = 8.936e-27
Identity = 114/416 (27.40%), Postives = 181/416 (43.51%), Query Frame = 1 
Query:  406 GTNYQKSITGLLEPYKDRKFYVVFHPTNSSYHMAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFVNDNRSKKIQLFNSGAHTGFVSFYS-HQQQVRFQPRSFLMVSKTTKEIKVTSISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEEKDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDITDFKLTLNEASSVRTIDRQTSQSSVTGNSTLTN-IENLNSKPMYMNSVSSYIDSINSLTAIVIQKNENYFPPTVIYRTSYTGILIRNDNIFQVQWVISDMFLMSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFKPTVVGRLKCKCNVKVLKDNSDSFMEKELDFYG 1647
            G     +I   L P  + +  VVF PT+  Y   +IVV  + K+FK++LFGYA  A++           Q P +LL   +    NRS ++ L N    T F+ FY  ++  + F+PR FL+    T+ +++          +  LY DE LRRA +  C   P Q    LFN   IF   P E D    K    ++ P L +Q++ +     I L   I   +R +C   D  D   T++  ++V   + +  +     N  +TN I+NL +             S   LT ++I+KNE  FP T I   S  GI+I+N N   + W I  +       +P                 D    +   KGT+   D+ ++P+ F+P  VG+   +  V+V  ++  S +E  +   G
Sbjct:  177 GRKLSSAIDVSLLPKVENRITVVFLPTDHWYCSGNIVVECQSKKFKVHLFGYACPAKIVF---------QEPKRLLYNSENEESNRSVEMSLTNVSPRTAFIYFYDVYKSGITFRPRKFLVHPDETRSVEIILPRIEKQYEYGVLYVDEGLRRATKEYCNKDP-QKMPKLFNDLRIFFPCPGESDRNYEKWRSTNYVPELNKQIEKNFFEKTIELS-KIKYLSREDCRLLDSVDV-FTMDGLTTVSFPNLKVDEDKY-NNVVITNYIKNLRT------------SSKGELTNVLIEKNEYEFPETPIKSKSVIGIVIQNPNGNPLSWSIVHI-------NPH----------------DYMAVIGESKGTM--SDNKVIPIQFQPPSVGKFVSEFIVRVWNNDDYSQIENLIKMTG 542
The following BLAST results are available for this feature:
BLAST of SMED30018491 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018491 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000000384.14.374e-1599.81SMESG000000384.1[more]
SMESG000000384.13.005e-999.81SMESG000000384.1[more]
SMESG000072635.18.936e-2727.40SMESG000072635.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018491 ID=SMED30018491|Name=SMED30018491|organism=Schmidtea mediterranea sexual|type=transcript|length=2164bp
GGAGGAGGGGGCAGAGTAGGTGAGAGGGGACGATGCATTAGTAGTGATGA
GAATTGGGAGTTAATTATTTTCAATCTCCAAGGTTTAGTGTTGTCATTTG
TTACTAGATCAAAACCCAAATATGAGGAAATCCGTGCTTGGTTGCAACAC
AATCAAATGTAAGATTATAATCGTAAATTGTTGTTATTATTAGGTTTCTG
TTTGTTGTGCCTATTTTCTGCTAAGCTGGAAACTTAATTTCTTCATATCC
TAGCATTAAATCTTTCAAACTCCACCTGAAAGCCGAGAAACCACAATTTG
CTGAGATTCGAATTATTTCGCCGTCCACCCCTTTCCATGTTAACATTTTT
GTTTTTTCTCATAACTGCAATTACATTTCCCCACAACTAGTTCACCAATC
AGTCAGGAACCAATTACCAAAAGTCGATAACGGGTTTACTTGAGCCATAT
AAAGATAGAAAGTTCTACGTCGTATTTCACCCGACGAATTCCTCGTATCA
CATGGCGCATATTGTGGTGACCTTGGAGGATAAACGGTTCAAAATGTATC
TGTTTGGCTATGCAGAAGCCGCTAGAGTTGAATTGCTTCATTTCCAACCG
AGTTCCAATCCCCAAAATCCCGCTAAATTATTATTGATACGGGATTTCGT
AAATGATAATCGTAGCAAAAAAATCCAGTTGTTTAATTCTGGAGCTCATA
CTGGCTTTGTTTCTTTCTATTCTCACCAGCAACAGGTCCGATTCCAACCG
AGATCCTTCTTAATGGTCTCGAAAACGACAAAAGAAATTAAGGTTACATC
AATTTCTCCGGTTGATGTGATAACGTTTTTCTTTCTTTACTGTGATGAGG
AGTTGCGCCGTGCTTGTAGAATCAGCTGCACTACATTTCCCCATCAAGAT
AAATATAACCTTTTCAACGTGGCGCATATCTTTACAATTCTTCCGGAAGA
GAAAGATATTCAGCAATGTAAGTTGCCGGAATTTGATTGGCGGCCTAGTC
TCATTCGTCAACTTGATGGTTCCGTTTGTTATTGTAACATTAATCTCATG
CCTGCAATTTCTAAATCCACCAGACCGAATTGCGTTGATTTCGATATTAC
AGATTTTAAGTTGACTCTAAATGAAGCCAGTTCCGTGCGTACAATTGATC
GGCAAACTAGCCAATCATCTGTTACTGGAAATAGTACTTTGACAAATATT
GAGAATCTCAACAGCAAGCCCATGTACATGAATAGCGTTTCCAGCTATAT
CGACTCAATAAACTCATTGACCGCTATTGTCATACAGAAGAATGAGAATT
ATTTCCCTCCTACTGTAATCTATCGAACTTCATATACGGGCATTTTAATA
CGCAACGATAATATTTTCCAAGTCCAATGGGTGATTTCCGACATGTTCCT
CATGAGTTCCAATAATTCTCCTGACACTTGTAGTAGTAGATATTCTTTGT
CCAGTTGGTCTGGAACTATTCCAGACTCTTGTATGACTGTCACGATGTTT
AAAGGTACGCTGAAACCCGGAGATTCCGTTCTGGTTCCTGTTGCGTTTAA
ACCGACTGTTGTAGGACGCTTAAAATGCAAATGCAATGTGAAAGTTTTAA
AAGATAACAGTGATTCATTTATGGAAAAGGAACTCGATTTTTACGGGAAG
GGAATTGCACAGGACTTGGACTGCGAGCAGGATCAAAGGAATCTTAATTT
CAATGTCAGCCCGCAAATATTGAATTTCCTCAGTTTTGAGTCAGGGTTAC
CGGTCAAGCGGAAACTTTACCTTCAGAGTAACAGTGATTATCTACAGATT
TTCCTGAGTCATGTCGGAGAACCCTTTGAAATTAAAACGAAGAAAGTTAT
TGTTAAGAAAGGAAAAAGTCTTATTGAGATATCCTTCAAAGCCTTTAAAC
CCGACATCTTTAGCAACGTTCTGGTATTGAGTGATGATGAAGGAAATTCC
ACATTCGTTTTGCTGAAAGGAGAACTCAGATAGATAAATTATTATTATTA
TTATTAATTTGGCAATTATGCGGTAGATTTCTAACGGATTCCAGTTCACT
TTAGCGTGGCATTTTTCGACGATAATTTATGAATAATTTCACATAGAGAA
AATGTAATAAATCAGATTCTGAATTACACGAAAATTGTTTATTGAGAAAA
AAACCTCCCGATTC
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protein sequence of SMED30018491-orf-1

>SMED30018491-orf-1 ID=SMED30018491-orf-1|Name=SMED30018491-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=494bp
MAHIVVTLEDKRFKMYLFGYAEAARVELLHFQPSSNPQNPAKLLLIRDFV
NDNRSKKIQLFNSGAHTGFVSFYSHQQQVRFQPRSFLMVSKTTKEIKVTS
ISPVDVITFFFLYCDEELRRACRISCTTFPHQDKYNLFNVAHIFTILPEE
KDIQQCKLPEFDWRPSLIRQLDGSVCYCNINLMPAISKSTRPNCVDFDIT
DFKLTLNEASSVRTIDRQTSQSSVTGNSTLTNIENLNSKPMYMNSVSSYI
DSINSLTAIVIQKNENYFPPTVIYRTSYTGILIRNDNIFQVQWVISDMFL
MSSNNSPDTCSSRYSLSSWSGTIPDSCMTVTMFKGTLKPGDSVLVPVAFK
PTVVGRLKCKCNVKVLKDNSDSFMEKELDFYGKGIAQDLDCEQDQRNLNF
NVSPQILNFLSFESGLPVKRKLYLQSNSDYLQIFLSHVGEPFEIKTKKVI
VKKGKSLIEISFKAFKPDIFSNVLVLSDDEGNSTFVLLKGELR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000420parapharyngeal region
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR013783Ig-like_fold
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 256..386
e-value: 3.0E-6
score: 29.4