SMED30018479

Overview
NameSMED30018479
Smed IDSMED30018479
Length (bp)1459
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018479 (SMED30018479) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018479 (SMED30018479) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018479 (SMED30018479) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018479 (SMED30018479) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018479

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30018479h1SMcG0001302 SmedASXL_013430SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30018479h1SMcG0001302 dd_Smed_v4_12803_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30018479h1SMcG0001302 dd_Smed_v6_12803_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018479 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.026247:870..2379 +7196
v31.008511:13664..15170 +7186
Homology
[Close]
BLAST of SMED30018479 vs. Planmine SMEST
Match: SMESG000019113.1 (SMESG000019113.1)

HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0
Identity = 361/364 (99.18%), Postives = 362/364 (99.45%), Query Frame = 3 
Query:  294 MTIELTLYKINEIFKFINPXXXXXXXXXXXXNVVYFIQPPVELLIVTRIIHGISFVAINLLKESIFGYDNQTEKSNIVIYKRLIQSFSTICASIYVVLIQYVPVNKIVLTIQPTNISGIVLSTMWLIYSIFLLCNIFQSCKSKDITSIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLAPLGALVFDLSMKHISFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNFCVICGLXXXXXXXXXXXXXXLEQAVTLIMFLFGYFIVNVAQSGSYLLLHNMVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGILSLFWIGITVFSVIFYEISKKS 1385
            MTIELTLYKINEIFKFINPILLISS LGILSNVVYFIQPPVELLIVTRIIHGISFVAINLLKESIFGY NQTEKSNIVIYKRLIQSFSTICASIYVVLIQYVPVNKIVLTIQPTNISGIVLSTMWLIYSIFLLCNIFQSCKSKDITSIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLAPLGALVFDLSMKHISFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNFCVICGLISIAISIAAKSYSILEQAVTLIMFLFGYFIVNVAQSGSYLLLHNMVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGIL+LFWIGITVFSVIFYEISKKS
Sbjct:    1 MTIELTLYKINEIFKFINPILLISSVLGILSNVVYFIQPPVELLIVTRIIHGISFVAINLLKESIFGYVNQTEKSNIVIYKRLIQSFSTICASIYVVLIQYVPVNKIVLTIQPTNISGIVLSTMWLIYSIFLLCNIFQSCKSKDITSIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLAPLGALVFDLSMKHISFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNFCVICGLISIAISIAAKSYSILEQAVTLIMFLFGYFIVNVAQSGSYLLLHNMVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGILALFWIGITVFSVIFYEISKKS 364
[Close]
BLAST of SMED30018479 vs. Planmine SMEST
Match: SMESG000019107.1 (SMESG000019107.1)

HSP 1 Score: 652.514 bits (1682), Expect = 0.000e+0
Identity = 360/364 (98.90%), Postives = 362/364 (99.45%), Query Frame = 3 
Query:  294 MTIELTLYKINEIFKFINPXXXXXXXXXXXXNVVYFIQPPVELLIVTRIIHGISFVAINLLKESIFGYDNQTEKSNIVIYKRLIQSFSTICASIYVVLIQYVPVNKIVLTIQPTNISGIVLSTMWLIYSIFLLCNIFQSCKSKDITSIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLAPLGALVFDLSMKHISFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNFCVICGLXXXXXXXXXXXXXXLEQAVTLIMFLFGYFIVNVAQSGSYLLLHNMVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGILSLFWIGITVFSVIFYEISKKS 1385
            MTIELTLYKINEIFKFINPILLISSALGILSNVVYFIQPPVELLIVTRIIHGISFVAINLLKESIFGYDNQTEKSNIVIYKRLIQSFS ICASIYVVLIQYVPVNKIVLTIQPTNISGIVLS MWLIYSIFLLCNIFQSCKSKDITSIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLAPLGALVFDLSMKH+SFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNFCVICGLISIAISIAAKSYSILEQAVTLIMFLFGYFIVNVAQSGSYLLLHNMVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGIL+LFWIGITVFSVIFYEISKKS
Sbjct:    1 MTIELTLYKINEIFKFINPILLISSALGILSNVVYFIQPPVELLIVTRIIHGISFVAINLLKESIFGYDNQTEKSNIVIYKRLIQSFSIICASIYVVLIQYVPVNKIVLTIQPTNISGIVLSIMWLIYSIFLLCNIFQSCKSKDITSIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLAPLGALVFDLSMKHVSFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNFCVICGLISIAISIAAKSYSILEQAVTLIMFLFGYFIVNVAQSGSYLLLHNMVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGILALFWIGITVFSVIFYEISKKS 364
The following BLAST results are available for this feature:
BLAST of SMED30018479 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018479 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000019113.10.000e+099.18SMESG000019113.1[more]
SMESG000019107.10.000e+098.90SMESG000019107.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018479 ID=SMED30018479|Name=SMED30018479|organism=Schmidtea mediterranea sexual|type=transcript|length=1459bp
AAAAAGTTTTATGAAACATAACTGCAAGCAAATTAATATAATTGAAATTC
GTGTATGTAGGCTTGCTACAATGTGTTTCACGTTGAATCTGTTGAAACGC
AAATGAATTTTAGTTCTCAAAAAGCTCAAAAATGCTGAAGAACTTCATGT
TTTATTTGGTTAGTTTTATTTATTATTTCGATCACCTGGTTATTTGTGCA
ACCTATTGGTTTTATTTGTCAGAATATCTAAGTAACGAATTTCTTATCCA
AATTTACGGTTTCGTTTTATTGTGCCATGAAGTCTTGTTGAGAATGACAA
TCGAATTAACACTATATAAAATAAATGAAATTTTCAAATTTATCAATCCT
ATATTATTGATATCTTCAGCTCTGGGAATTCTATCAAACGTTGTCTATTT
CATCCAACCACCGGTCGAGTTATTAATAGTAACAAGAATCATTCATGGAA
TTTCATTCGTCGCTATAAATCTATTGAAAGAATCGATATTCGGATATGAC
AATCAAACGGAAAAATCGAACATTGTGATTTATAAGAGATTAATCCAGTC
ATTTTCAACAATTTGCGCATCAATTTATGTTGTATTGATACAGTATGTTC
CAGTAAATAAAATTGTGTTAACAATTCAACCTACGAATATTTCTGGAATT
GTTCTCTCAACAATGTGGTTGATTTACAGTATTTTTTTGCTGTGCAATAT
TTTCCAATCCTGCAAAAGCAAAGATATAACCAGCATTGATGAAGAATTCA
ATGAAAAACGATTATTGGAATTCGAAGCTGACTTGCATCAAAATAATATT
TTGTCAATATTTAATTTATATTATTTGTTTGCATTGTTGATTAATCTGTC
AACTAAATTCGCTCTCAATTTCGTTCTCGCACCCTTAGGAGCTTTGGTTT
TCGATTTGTCAATGAAACACATATCTTTCACATTTCTCGGTTTGAGTAGT
GCAAACTTTTTAGTGTATTTTTTACTAAAAATCAAAAGTGAATCAATAAG
CAATATTACGAAGTTAATTATTATGAATTTTTGTGTTATTTGTGGATTGA
TTTCAATTGCAATATCTATAGCTGCAAAATCGTACAGCATTTTAGAGCAA
GCAGTAACTTTGATAATGTTTCTTTTCGGATATTTTATAGTCAATGTTGC
ACAGTCTGGATCCTATTTACTGTTACACAATATGGTTATTTCTGATGTAA
CACCTCTCGAAAAACTGATAATTGTAAAAATGAGGCGATCATTCAAATTT
ATTGGCTGTATATTCGGAATATTAATGGGTCCCGTAGTCATTGTTTGGCC
AAGCTTAAGTATGGGAATTTTATCATTGTTTTGGATTGGAATCACAGTTT
TTAGTGTTATATTTTATGAAATATCTAAAAAAAGTTGAAAACAAAGAGTT
GTGGATAATCTCGACTATGTTATGTTTTGTTTGATTGTAAATACACCAGT
GCATTTTCT
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protein sequence of SMED30018479-orf-1

>SMED30018479-orf-1 ID=SMED30018479-orf-1|Name=SMED30018479-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=419bp
MLKNFMFYLVSFIYYFDHLVICATYWFYLSEYLSNEFLIQIYGFVLLCHE
VLLRMTIELTLYKINEIFKFINPILLISSALGILSNVVYFIQPPVELLIV
TRIIHGISFVAINLLKESIFGYDNQTEKSNIVIYKRLIQSFSTICASIYV
VLIQYVPVNKIVLTIQPTNISGIVLSTMWLIYSIFLLCNIFQSCKSKDIT
SIDEEFNEKRLLEFEADLHQNNILSIFNLYYLFALLINLSTKFALNFVLA
PLGALVFDLSMKHISFTFLGLSSANFLVYFLLKIKSESISNITKLIIMNF
CVICGLISIAISIAAKSYSILEQAVTLIMFLFGYFIVNVAQSGSYLLLHN
MVISDVTPLEKLIIVKMRRSFKFIGCIFGILMGPVVIVWPSLSMGILSLF
WIGITVFSVIFYEISKKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002032epidermal cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 263..282
NoneNo IPR availableTMHMMTMhelixcoord: 226..248
NoneNo IPR availableTMHMMTMhelixcoord: 396..415
NoneNo IPR availableTMHMMTMhelixcoord: 7..29
NoneNo IPR availableTMHMMTMhelixcoord: 168..190
NoneNo IPR availableTMHMMTMhelixcoord: 136..158
NoneNo IPR availableTMHMMTMhelixcoord: 327..349
NoneNo IPR availableTMHMMTMhelixcoord: 295..317
NoneNo IPR availableTMHMMTMhelixcoord: 70..92
NoneNo IPR availableTMHMMTMhelixcoord: 98..115
NoneNo IPR availableTMHMMTMhelixcoord: 370..392
NoneNo IPR availableTMHMMTMhelixcoord: 39..57