SMED30018404

Overview
NameSMED30018404
Smed IDSMED30018404
Length (bp)1579
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018404 (SMED30018404) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018404 (SMED30018404) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018404 (SMED30018404) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018404 (SMED30018404) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018404

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30018404SMESG000035739.1 Contig2727GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018404SMESG000032486.1 Contig2727GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
protonephridiaSMED30018404SMESG000032486.1 dd_Smed_v4_4574_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30018404SMESG000032486.1 dd_Smed_v4_4574_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018404SMESG000032486.1 dd_Smed_v4_4574_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30018404SMESG000032486.1 dd_Smed_v4_4574_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018404SMESG000032486.1 dd_Smed_v4_4574_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30018404 vs. Planmine SMEST
Match: SMESG000032486.1 (SMESG000032486.1)

HSP 1 Score: 850.507 bits (2196), Expect = 0.000e+0
Identity = 455/458 (99.34%), Postives = 456/458 (99.56%), Query Frame = 1 
Query:   94 MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPEDEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLKIMKHDXXXXXXXXXXXXXQTNPQDVEEQIKPIVRNLDQNIPIQNESQKSNEVINDIIEEPIIPVKHKQVKIELPKKNNFNMNKDELEKTLNKLSKTLTFDNNFSKINIFDRQLIFEGEVKALEDLQTTPGILKKISSGRMLKMNLILFDNLFVXXXXXXXXXXXVIECCSPDMIEAVTYFFPGNLRSNFVKPNQTLSCISKNASKNDISNHLFVLRIKIEVATVIKDPLILTAPTIERRDQWVSIISNTS 1467
            MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPEDEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLKIMKHDEESISNE NSVENQTNPQDVEEQIKPIVRNLDQNIPIQNESQKSNEVINDIIEEPIIPV HKQVKIELPKKNNFNMNKDELEKTLNKLSKTLTFDNNFSKINIFDRQLIFEGEVKALEDLQTTPGILKKISSGRMLK+NLILFDNLFVLSLKKKGKKLKVIECCSPDMIEAVTYFFPGNLRSNFVKPNQTLSCISKNASKNDISNHLFVLRIKIEVATVIKDPLILTAPTIERRDQWVSIISNTS
Sbjct:    1 MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPEDEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLKIMKHDEESISNEKNSVENQTNPQDVEEQIKPIVRNLDQNIPIQNESQKSNEVINDIIEEPIIPVTHKQVKIELPKKNNFNMNKDELEKTLNKLSKTLTFDNNFSKINIFDRQLIFEGEVKALEDLQTTPGILKKISSGRMLKLNLILFDNLFVLSLKKKGKKLKVIECCSPDMIEAVTYFFPGNLRSNFVKPNQTLSCISKNASKNDISNHLFVLRIKIEVATVIKDPLILTAPTIERRDQWVSIISNTS 458
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BLAST of SMED30018404 vs. Planmine SMEST
Match: SMESG000032486.1 (SMESG000032486.1)

HSP 1 Score: 456.062 bits (1172), Expect = 2.247e-160
Identity = 246/247 (99.60%), Postives = 246/247 (99.60%), Query Frame = 1 
Query:   94 MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPEDEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLKIMKHDXXXXXXXXXXXXXQTNPQDVEEQIKPIVRNLDQN 834
            MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPEDEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLKIMKHDEESISNE NSVENQTNPQDVEEQIKPIVRNLDQN
Sbjct:    1 MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPEDEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLKIMKHDEESISNEKNSVENQTNPQDVEEQIKPIVRNLDQN 247
[Close]
BLAST of SMED30018404 vs. Planmine SMEST
Match: SMESG000044434.1 (SMESG000044434.1)

HSP 1 Score: 116.316 bits (290), Expect = 4.109e-27
Identity = 69/188 (36.70%), Postives = 99/188 (52.66%), Query Frame = 1 
Query:  283 VKRTVKTYQRKVIRKTGPNGEVTEEIIESGD------------AIPNHEFPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQLTHDSDDN-------------------------------HSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLSVFGKSDLK 717
            +KRT KTY R++IRK GPNGEV+EEIIE GD            ++ N EFP    + N  +   PK+ G ++ QA+ G +S   SGE+ +M+ STGPMQ+      N                                  P G+ V +KF+ +K+ TVTHSST+NG++ + S+KS F+S  G+S L+
Sbjct:   85 IKRTTKTYCRRIIRKIGPNGEVSEEIIEEGDPPANFFPPTSQMSLQNPEFPSITESANNSDLSTPKIAGGTKFQATAGFQSVSQSGEQKSMQLSTGPMQINFKDSGNRMLSLENNRSLDSVDTDQQLGLVAGSGNSVQQVLPDGTIVTRKFYGEKSATVTHSSTINGQT-LTSSKSAFMSFSGESMLE 271

HSP 2 Score: 50.0618 bits (118), Expect = 4.049e-6
Identity = 51/179 (28.49%), Postives = 83/179 (46.37%), Query Frame = 1 
Query:  940 KKNNFNMNKDELEKTLNKLSKTLTFDNNFSKINIFDRQLIFEGEVKALEDLQTTPGILKKISSGRMLKMNLILFDNLFVXXXXXXXXXXXVIECCSPDMIEAVTYF-------FPGNLRSNFVKPNQTLSCISKNASKNDISNHLFVLRIKIEVATVIKDPLILTAPTIERRDQWVSII 1455
            ++N F M   E +  L  L K L F N+   + I    LI +GEV+A  + Q +   LKK+S  R     LI+   L + + KK   K  V+E C    ++A  +         P   R++        S  S +AS   +    FVL+ + +  +++ + L+LT PT E R+ W++ I
Sbjct:  621 EENKFKM---EQKARLIDLCKFLEFRNSSRHVAIGSMDLIKDGEVRAAAN-QISSNALKKLSKKRQPTFYLIILSELVIFAKKKSQTKFVVLEHCPRSKVQASIHVDFATSISGPKASRASIGSQLSISSSSSYDASTQAVIGQPFVLKFEKDADSLLPESLVLTVPTSEIRESWINAI 795
The following BLAST results are available for this feature:
BLAST of SMED30018404 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018404 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000032486.10.000e+099.34SMESG000032486.1[more]
SMESG000032486.12.247e-16099.60SMESG000032486.1[more]
SMESG000044434.14.109e-2736.70SMESG000044434.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018404 ID=SMED30018404|Name=SMED30018404|organism=Schmidtea mediterranea sexual|type=transcript|length=1579bp
TTTAAATATATCAAAAGTATTTAAACAGTAAAGTTAAAGTTTTACAAAAA
CAACTTAAAATTAAAAAAAATTTGCTAAATTGAACATTGTCAAATGTCAT
CTCCGAATAATTTCATGAAAAATGAATCGAACGATTCTAATAACATAAAC
ATTCCAACTAAGACTATCGAATCCAGGATCCTTTCACAGAAAGCCGAAGA
GACTTGTATTCAACAAAAGTTTTCCAGATTGAAAACACCGGAAGATGAAG
AGATAATGTTGAAAAACTCAGCGAATCCTTTAGTAAAAAGAACTGTCAAG
ACTTATCAACGAAAAGTTATCCGTAAAACCGGACCAAACGGAGAAGTGAC
CGAGGAAATCATTGAATCTGGAGATGCTATTCCCAATCATGAATTTCCAA
TGAAAGATACGACAGAGAATATTGATGAGAAACCAAAACCGAAAATGTAC
GGAAAATCTGAAATTCAAGCTTCATATGGAGTCAGATCGACAATGGCATC
AGGTGAAGAGGCTAATATGGAATATTCAACTGGACCAATGCAGTTGACCC
ATGATAGTGATGACAACCATTCATATCCTACCGGATCAACAGTTATGAAG
AAGTTCCACCAAGATAAAACAACAACAGTTACTCATTCGTCGACAGTTAA
TGGTGAATCCAAAGTGTTGTCTACAAAATCAACATTTCTCTCCGTCTTTG
GAAAAAGTGATTTAAAAATCATGAAACATGATGAAGAGAGCATTTCAAAT
GAAAACAATTCAGTTGAAAATCAGACGAATCCTCAAGATGTTGAAGAACA
AATAAAGCCAATTGTAAGAAATCTTGATCAAAATATACCAATTCAAAATG
AGTCTCAAAAATCAAATGAGGTTATCAATGACATTATTGAGGAACCAATA
ATACCAGTTAAGCATAAACAAGTCAAAATCGAATTACCGAAAAAAAATAA
TTTCAACATGAATAAAGACGAGTTGGAGAAGACATTGAATAAACTATCGA
AAACCTTAACATTTGACAATAATTTTTCAAAAATAAATATCTTCGATAGA
CAATTAATATTTGAAGGCGAAGTCAAAGCATTAGAAGATTTACAAACAAC
TCCGGGAATTTTGAAAAAGATTTCAAGCGGAAGAATGCTCAAAATGAATT
TGATTTTGTTTGATAATTTATTTGTATTATCTTTGAAAAAAAAGGGCAAA
AAGCTTAAAGTTATAGAATGTTGTAGTCCAGATATGATTGAAGCGGTTAC
TTATTTTTTCCCCGGAAACCTGAGAAGTAACTTCGTTAAACCAAATCAAA
CACTCTCCTGTATTTCAAAAAATGCATCCAAGAATGATATCTCCAATCAT
TTATTTGTGCTAAGAATCAAGATTGAAGTTGCAACCGTAATTAAAGATCC
ATTAATTTTGACAGCTCCGACAATAGAAAGACGAGATCAGTGGGTTTCGA
TAATTTCAAATACATCATAATAAAAACAGTAGAGATATGATAAACAAAAT
TTTACCGGGTGTTGAATGATGAAGAATATATTTCAGTTTTATTTTCTGTT
CTAAATAAATGTTGATTTTAATATTATAA
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protein sequence of SMED30018404-orf-1

>SMED30018404-orf-1 ID=SMED30018404-orf-1|Name=SMED30018404-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=459bp
MSSPNNFMKNESNDSNNINIPTKTIESRILSQKAEETCIQQKFSRLKTPE
DEEIMLKNSANPLVKRTVKTYQRKVIRKTGPNGEVTEEIIESGDAIPNHE
FPMKDTTENIDEKPKPKMYGKSEIQASYGVRSTMASGEEANMEYSTGPMQ
LTHDSDDNHSYPTGSTVMKKFHQDKTTTVTHSSTVNGESKVLSTKSTFLS
VFGKSDLKIMKHDEESISNENNSVENQTNPQDVEEQIKPIVRNLDQNIPI
QNESQKSNEVINDIIEEPIIPVKHKQVKIELPKKNNFNMNKDELEKTLNK
LSKTLTFDNNFSKINIFDRQLIFEGEVKALEDLQTTPGILKKISSGRMLK
MNLILFDNLFVLSLKKKGKKLKVIECCSPDMIEAVTYFFPGNLRSNFVKP
NQTLSCISKNASKNDISNHLFVLRIKIEVATVIKDPLILTAPTIERRDQW
VSIISNTS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 284..304