SMED30018355

Overview
NameSMED30018355
Smed IDSMED30018355
Length (bp)1961
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018355 (SMED30018355) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018355 (SMED30018355) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018355 (SMED30018355) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018355 (SMED30018355) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018355

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30018355h1SMcG0022159 dd_Smed_v4_10379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30018355h1SMcG0022159 dd_Smed_v4_10379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018355 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003047:36411..42092 -8113
Homology
[Close]
BLAST of SMED30018355 vs. TrEMBL
Match: A0A3R7AKB1 (Uncharacterized protein OS=Aphanomyces astaci OX=112090 GN=DYB35_004616 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.541e-8
Identity = 38/118 (32.20%), Postives = 55/118 (46.61%), Query Frame = 3 
Query: 1146 LIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSH--DVEDTWNCSKCKMKNPFDRYRCHQCD-GLAGFF----DKWVCEK----CSFFNEKLDRNCYVCDLVNSNL 1466
            +++K N   E   D W C  C+F+ND     C +C  N P ++T    +V  TW C  C   NP     C  C+ G +G      DKW+C+     C++FN K    C VC+    +L
Sbjct: 3774 MVDKSNKPDEHTQDPWLCVVCTFQNDGLVTSCVMC--NEPRDHTKQGAEVAQTWQCENCTFINPIAETTCAVCEMGASGRREVPKDKWICDPEQGGCTYFNLKTAFYCDVCNRARPDL 3889
[Close]
BLAST of SMED30018355 vs. TrEMBL
Match: A0A418E4F8 (Uncharacterized protein OS=Aphanomyces astaci OX=112090 GN=DYB37_005301 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.541e-8
Identity = 38/118 (32.20%), Postives = 55/118 (46.61%), Query Frame = 3 
Query: 1146 LIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSH--DVEDTWNCSKCKMKNPFDRYRCHQCD-GLAGFF----DKWVCEK----CSFFNEKLDRNCYVCDLVNSNL 1466
            +++K N   E   D W C  C+F+ND     C +C  N P ++T    +V  TW C  C   NP     C  C+ G +G      DKW+C+     C++FN K    C VC+    +L
Sbjct: 3774 MVDKSNKPDEHTQDPWLCVVCTFQNDGLVTSCVMC--NEPRDHTKQGAEVAQTWQCENCTFINPIAETTCAVCEMGASGRREVPKDKWICDPEQGGCTYFNLKTAFYCDVCNRARPDL 3889
[Close]
BLAST of SMED30018355 vs. TrEMBL
Match: A0A397DRG3 (Uncharacterized protein OS=Aphanomyces astaci OX=112090 GN=DYB30_002991 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.541e-8
Identity = 38/118 (32.20%), Postives = 55/118 (46.61%), Query Frame = 3 
Query: 1146 LIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSH--DVEDTWNCSKCKMKNPFDRYRCHQCD-GLAGFF----DKWVCEK----CSFFNEKLDRNCYVCDLVNSNL 1466
            +++K N   E   D W C  C+F+ND     C +C  N P ++T    +V  TW C  C   NP     C  C+ G +G      DKW+C+     C++FN K    C VC+    +L
Sbjct: 3775 MVDKSNKPDEHTQDPWLCVVCTFQNDGLVTSCVMC--NEPRDHTKQVAEVAQTWQCENCTFINPIAETTCAVCEMGASGRREVPKDKWICDPEQGGCTYFNLKTAFYCDVCNRARPDL 3890
[Close]
BLAST of SMED30018355 vs. TrEMBL
Match: W4GLA5 (Uncharacterized protein OS=Aphanomyces astaci OX=112090 GN=H257_06741 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.545e-8
Identity = 38/118 (32.20%), Postives = 55/118 (46.61%), Query Frame = 3 
Query: 1146 LIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSH--DVEDTWNCSKCKMKNPFDRYRCHQCD-GLAGFF----DKWVCEK----CSFFNEKLDRNCYVCDLVNSNL 1466
            +++K N   E   D W C  C+F+ND     C +C  N P ++T    +V  TW C  C   NP     C  C+ G +G      DKW+C+     C++FN K    C VC+    +L
Sbjct: 3812 MVDKSNKPDEHTQDPWLCVVCTFQNDGLVTSCVMC--NEPRDHTKQVAEVAQTWQCENCTFINPIAETTCAVCEMGASGRREVPKDKWICDPEQGGCTYFNLKTAFYCDVCNRARPDL 3927
[Close]
BLAST of SMED30018355 vs. TrEMBL
Match: A0A3R7ZHI3 (Uncharacterized protein OS=Aphanomyces astaci OX=112090 GN=B5M09_007115 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.545e-8
Identity = 38/118 (32.20%), Postives = 55/118 (46.61%), Query Frame = 3 
Query: 1146 LIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSH--DVEDTWNCSKCKMKNPFDRYRCHQCD-GLAGFF----DKWVCEK----CSFFNEKLDRNCYVCDLVNSNL 1466
            +++K N   E   D W C  C+F+ND     C +C  N P ++T    +V  TW C  C   NP     C  C+ G +G      DKW+C+     C++FN K    C VC+    +L
Sbjct: 3812 MVDKSNKPDEHTQDPWLCVVCTFQNDGLVTSCVMC--NEPRDHTKQVAEVAQTWQCENCTFINPIAETTCAVCEMGASGRREVPKDKWICDPEQGGCTYFNLKTAFYCDVCNRARPDL 3927
[Close]
BLAST of SMED30018355 vs. Planmine SMEST
Match: SMESG000022833.1 (SMESG000022833.1)

HSP 1 Score: 1194.49 bits (3089), Expect = 0.000e+0
Identity = 605/611 (99.02%), Postives = 607/611 (99.35%), Query Frame = 3 
Query:   78 MNLENILNTDLRYKIIFYLKDVLKSSISKINNVDEXXXXXXXXXXCTDELKELDEEVLYVNLAASKAISQLAWNAYQNNGLYKDCHINAQYLEKIYFIMGNSNLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEIDNLMFKSCIYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLSSSSSNPTDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKSRNQEVFHGSNLTSFNDSFGLPEDKNQASKEYKDLNSSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSCIKENNYRGYGTSTPVNDISSNTSSRNEQDGKYSKNRIPIEEASNKITGKDSKLKQFHWYCINKKCSAINFSMAKYCSKCFVKNECASSNSFFIVLKNDKEVKMCRNCESVYVTSYCHCHKMMVTQV 1910
            MNLENILNTDLRYKIIFYLKDVLKSSISKINNVDEFIKFFFKDFKCTDELK+LDEEVLYVNLAASKAISQLAWNAYQNNGLYKDCHINAQYLEKIYFIMGN NLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEIDNLMFKSCIYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLSSSSSNPTDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKSRNQEVFHGSNLTSFNDSFGLPEDKNQASKEYKDLNSSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYEN SHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSCIKEN+YRGYGT TPVNDISSN SSRNEQDGKYSKNRIPIEEASNKITGKDSKLKQFHWYCINKKCSAINFSMAKYCSKCFVKNECASSNSFFIVLKNDKEVKMCRNCESVYVTSYCHCHKMMVTQV
Sbjct:    1 MNLENILNTDLRYKIIFYLKDVLKSSISKINNVDEFIKFFFKDFKCTDELKKLDEEVLYVNLAASKAISQLAWNAYQNNGLYKDCHINAQYLEKIYFIMGNCNLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEIDNLMFKSCIYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLSSSSSNPTDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKSRNQEVFHGSNLTSFNDSFGLPEDKNQASKEYKDLNSSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENISHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSCIKENDYRGYGTPTPVNDISSNKSSRNEQDGKYSKNRIPIEEASNKITGKDSKLKQFHWYCINKKCSAINFSMAKYCSKCFVKNECASSNSFFIVLKNDKEVKMCRNCESVYVTSYCHCHKMMVTQV 611
[Close]
BLAST of SMED30018355 vs. Planmine SMEST
Match: SMESG000022832.1 (SMESG000022832.1)

HSP 1 Score: 134.42 bits (337), Expect = 1.009e-32
Identity = 95/301 (31.56%), Postives = 141/301 (46.84%), Query Frame = 3 
Query: 1005 FWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEK-ENFNIEGNSDK------WSCNQCSFKNDKTDLLCSLCYA--NNPYENTSHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEK---NDVIEQDNEFCTSCIKENNYRGYGTST-----PVNDISSNTSSRNEQDGKYSKN----RIPIEEASNKITGKDSKLK----------------------QFHWYCINKKCSAINFSMAKYCSKCFVK 1778
            FW+C  CS+  NK     C  C    +++  +     +KIS  E K L E  EN  +  N+ +      W C QCS+ N+K+  +C +C+    NP +      E+TW C+KC   N FD Y+CH+C  +A F DKWVC +CSF+N+KL   C VCD+ N +LIKC+KC K   N    Q+N+ C +C        Y   +     P     S  + +N  D K   N       + + +      D+K K                      +  W+C+N+ C ++NF+  K+C KC VK
Sbjct:  290 FWQCAKCSY-INKTSAVTCETCGDTARNKRKANELQANKIS--EMKTLFEPGENAPVPNNASRIDNYNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCHKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPHLIKCRKCRKGFVNAPNNQNNDICGACKVSQKTDYYSLPSNNAIKPSPSPGSGGAKKNINDRKTGNNLSLQNANVTQPALSSGSGDTKRKSCKNCRKELQPNSNNNWCITCEKMKWFCLNEACDSVNFN-KKFCKKCQVK 586
[Close]
BLAST of SMED30018355 vs. Planmine SMEST
Match: SMESG000022828.1 (SMESG000022828.1)

HSP 1 Score: 131.724 bits (330), Expect = 1.494e-31
Identity = 94/300 (31.33%), Postives = 138/300 (46.00%), Query Frame = 3 
Query: 1008 WECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEK-ENFNIEGNSDK------WSCNQCSFKNDKTDLLCSLCYA--NNPYENTSHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEK---NDVIEQDNEFCTSCIKENNYRGYGTST-----PVNDISSNTSSRNEQDGKYSKN----RIPIEEASNKITGKDSKLK----------------------QFHWYCINKKCSAINFSMAKYCSKCFVK 1778
            WEC  CS+  NK     C  C    +++  +     +KIS  E K L E  EN  +  N+ +      W C QCS+ N+K+  +C +C+    NP +      E+TW C+KC   N FD Y+C +C G+A F DKWVC +CSF+N+KL   C VCD+ N  LIKC+KC K   N    Q+N+ C +C        Y         P     S  + +N  D K   N       + + +      D+K K                      +  W+C+N+ C ++NF + ++C KC VK
Sbjct:  466 WECAKCSY-INKISAVTCETCGDTARNKRKANELQANKIS--EMKTLFEPAENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCRGIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGACEISQKTDYYSLPIRIAIKPSPSPGSGGAKKNINDRKTGNNLSLQNANVTQPALSPGSGDTKRKSCKHCRKELQPNSNNNWCITCEKMKWFCLNEACDSVNFKV-EFCKKCQVK 761

HSP 2 Score: 68.1662 bits (165), Expect = 1.502e-11
Identity = 135/595 (22.69%), Postives = 224/595 (37.65%), Query Frame = 3 
Query:  132 LKDVLKSSISKINNVDEXXXXXXXXXXCTDELKELDEEVLYVNLAASKAISQLAWNAY-QNNGLY-KDCHINA--------QYLEKIYFIMGNSNLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEI-----DNLMFKSCIYCDCVVIGDICSVCEGN---------SF----VLKDKSGDPMTFDF---------------PFITDNVTDLKNQDLSSSSSNP--TDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAI------------ELNHKSRNQEVFHGSNLTSF---------NDSFGLPEDKNQASKEYKDLNSSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEKENFNIEGNSDK------WSCNQCSFKNDKTDLLCSLCY---ANNPYENTS--------HDVEDTWNCSKCKMKNPFDRYRCHQCDGLA------------------GFFDK------------------WVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSCIKENNYRGY 1559
            LK+ ++  IS IN +D F              +  D + LY  L  +K I+QL  N Y +NN LY K C  N         +Y+EKI        +  +Y+D         E+ L+E+++ Y+II+ +L  +E      DN++   CIYCD ++   IC VC+           SF      K+KS +  T++F                    NV + K    +S + N   T  ++    S+KT D   LN T       ++   +S              ++N+ + +Q      +  S           D FG   +K  ++K    + S+ N+S L+    +  + +      W C  CS+      ++  +    D+    ++E+   +K+  ++  I +  EN     N+++      WSC QCSFKN K+   C  C     N   E  S         +  D+W C+KC   N      C  C   A                    F+                   W C +CS+ NEK    C +C  ++ N  K     + + I ++   C  C   N++  Y
Sbjct:   22 LKEKIRQKISCINQIDYFPVEVQFKLSKDQRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNMPFDKYMEKI------PKMNDSYID---------ESQLVEIIAIYVIIYSLLLNQEKILDKDDNMV---CIYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESKQTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRADRFGKESKTNNIKYKDEKKKHKINNDNSDQPSVKTPDCYSLYERTCEDQRADGFGNKSNKTISAKTV--IASNTNLSTLANTKSIVCDNK------WACSKCSYENETSDVKCVTCSHTDRNGGKETES-GSNKLPESQS-ITVTHENAPTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYTDRNRGKETKSRSDKLLEMQNSVDSWECAKCSYINKISAVTCETCGDTARNKRKANELQANKISEMKTLFEPAENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGK-----EAEGIPEETWRCNKCGNNNSFDVY 583
[Close]
BLAST of SMED30018355 vs. Planmine SMEST
Match: SMESG000022823.1 (SMESG000022823.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.543e-8
Identity = 104/462 (22.51%), Postives = 177/462 (38.31%), Query Frame = 3 
Query:  219 DELKELDEEVLYVNLAASKAISQLAWNAYQNNGLYKDCHINAQYLEKIYFIMGNSNLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEIDNLMFKSCIYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLSSSSSNPTDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKSRNQEVFHGSNLTSFNDSFGLPEDKNQASKEYKDLN-SSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDK--QSQIDSENKNMDKISVTEDKILIEKEN----FNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENTSHD---VE--------------DTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSC 1532
            DE      + LY+ L   +  +Q+  N Y     Y+ C        K+  I  + N   N     +   E   ++++E +  ++  H ++Y +E+   + K+    D ++IG IC  CE             M  D      NV +    D++  ++N  + L  V      K +    +   + ++      D    LN  + + ++ +     S+ +   +    N+  K  ++L  + ENI K S DF           + W+C  C       + +FC  CL +K   +Q   +  N++K      K  +EK N    +  E  S   S  +C    ++T+  C     N+   N +H    VE              D W C KC M N +D + C  C         W CE+C   N+     C+ C   N N+  CK+C K+  I  D  FC +C
Sbjct:    9 DEYNNYSTDNLYLELRNCRKKAQILLNLYHCPKEYEKC--------KLLDIGSHLNNTNNKPFGIENVQEINSDIIVEAIGEFVKYHHVIYERELQVELDKNKEITD-LLIGSICCFCE-------------MRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDKVPNF------DPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKINEENIVKTSKDF-----------SEWKCSKCGL---LNYDEFCMACLNEKVITNQKYGQEINLEKSGA---KNSVEKRNTTSTYRYENVSSIES-EKCVICRNETNCNCKSNTKNSAITNENHKETLVENWENNDASTTNINSDQWKCDKCGMLN-YDDF-CSACLNERRTIKLWKCERCKKLNDFFIEKCFSCGKTNENIQLCKQC-KSIKIAADRRFCDTC 421
The following BLAST results are available for this feature:
BLAST of SMED30018355 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3R7AKB15.541e-832.20Uncharacterized protein OS=Aphanomyces astaci OX=1... [more]
A0A418E4F85.541e-832.20Uncharacterized protein OS=Aphanomyces astaci OX=1... [more]
A0A397DRG35.541e-832.20Uncharacterized protein OS=Aphanomyces astaci OX=1... [more]
W4GLA55.545e-832.20Uncharacterized protein OS=Aphanomyces astaci OX=1... [more]
A0A3R7ZHI35.545e-832.20Uncharacterized protein OS=Aphanomyces astaci OX=1... [more]
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BLAST of SMED30018355 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018355 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000022833.10.000e+099.02SMESG000022833.1[more]
SMESG000022832.11.009e-3231.56SMESG000022832.1[more]
SMESG000022828.11.494e-3131.33SMESG000022828.1[more]
SMESG000022823.11.543e-822.51SMESG000022823.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018355 ID=SMED30018355|Name=SMED30018355|organism=Schmidtea mediterranea sexual|type=transcript|length=1961bp
CATAACCTTGAAACTACAAGCTGCATGCATCAGTTTTTATTTGATATTTT
ATACGAAATCACAATGTAAAGCTTTTAATGAATTTGGAAAATATTTTAAA
TACTGACTTAAGATACAAGATTATTTTCTACCTAAAGGATGTGCTTAAGT
CTAGCATTTCAAAAATTAATAATGTGGATGAGTTTATAAAGTTTTTTTTC
AAAGACTTTAAATGCACTGATGAATTGAAGGAATTAGACGAAGAAGTATT
ATATGTTAATTTGGCAGCAAGTAAAGCAATTTCGCAATTGGCTTGGAATG
CTTACCAAAATAATGGCTTATATAAAGATTGTCATATTAACGCCCAGTAT
TTGGAAAAGATTTATTTCATTATGGGCAACAGCAATTTATATACCAATTA
TTTAGACAAATTTAAGTTGGGCACAGAATTCTTGGAAAATGTGTTGATTG
AAATGCTATCTAGATACATCATTATACATTGTATTTTATATTATAAAGAG
ATTGATAATTTAATGTTTAAATCGTGTATATATTGCGATTGTGTAGTTAT
AGGAGACATTTGTTCTGTGTGTGAAGGGAATTCATTTGTCTTAAAGGACA
AAAGTGGGGATCCAATGACTTTTGATTTTCCATTTATAACTGATAATGTA
ACTGATTTGAAAAATCAAGATTTGTCTTCATCAAGTTCTAATCCAACTGA
TTGTCTTGAAAGCGTTTCTGATTCAATTAAAACCAAAGACAATGATGAAT
TAAACTCTACCATAAATATTAGTGATTATACAGAAAAAATTGTAAGTGAT
TCTGCTATTGAATTGAACCACAAATCGAGAAATCAAGAGGTTTTCCATGG
TAGCAATCTTACATCATTTAATGATTCATTCGGTTTACCTGAGGACAAAA
ATCAAGCTTCGAAAGAGTATAAAGATCTCAACTCTTCCGAAAACATTTCA
AAATTATCTCCTGATTTTGATTTGGCAACTGAAATTGAACAAACTAAGGA
AACATTTTGGGAATGCAAATCTTGTAGTTTCAATAAAAACAAAGAACATA
TGCAATTTTGTTCGGTTTGCTTATTTGACAAACAATCACAAATTGACTCG
GAAAATAAAAATATGGACAAAATTAGTGTGACGGAGGATAAAATTTTAAT
TGAAAAAGAAAATTTTAACATTGAAGGAAATTCAGATAAATGGTCATGTA
ATCAGTGTTCCTTCAAAAACGATAAAACTGATTTGCTTTGTTCTTTGTGT
TATGCAAATAATCCATACGAAAATACTTCCCATGACGTCGAAGATACGTG
GAATTGTTCCAAATGCAAAATGAAAAATCCGTTCGATCGGTATAGATGTC
ATCAATGTGATGGATTGGCTGGGTTTTTTGATAAATGGGTTTGTGAAAAA
TGTAGTTTTTTCAACGAAAAACTCGATAGAAATTGTTATGTCTGTGACCT
TGTGAATTCTAATTTAATCAAATGTAAAAAATGTGAAAAAAATGACGTTA
TTGAACAAGATAATGAATTTTGCACTTCATGCATAAAAGAAAACAACTAT
AGAGGTTATGGAACTTCCACCCCAGTGAATGATATCTCTTCCAATACATC
TTCAAGAAATGAACAAGATGGGAAATATTCAAAAAATCGGATTCCTATTG
AAGAAGCTTCAAATAAAATCACTGGAAAAGATTCAAAGTTGAAACAATTT
CATTGGTACTGCATTAATAAAAAATGCTCTGCCATTAATTTCTCGATGGC
GAAATATTGTTCTAAATGTTTTGTGAAGAATGAATGCGCGTCGTCGAATT
CTTTTTTCATTGTCTTAAAAAATGATAAAGAAGTGAAAATGTGTCGCAAC
TGTGAGTCTGTTTACGTTACTAGTTATTGTCATTGTCATAAAATGATGGT
AACTCAAGTATGAATGTGCCATATTATTGTACTAAACTATTTTCACTTGC
TAATTAAAGAG
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protein sequence of SMED30018355-orf-1

>SMED30018355-orf-1 ID=SMED30018355-orf-1|Name=SMED30018355-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=612bp
MNLENILNTDLRYKIIFYLKDVLKSSISKINNVDEFIKFFFKDFKCTDEL
KELDEEVLYVNLAASKAISQLAWNAYQNNGLYKDCHINAQYLEKIYFIMG
NSNLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEIDNLMFKSC
IYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLS
SSSSNPTDCLESVSDSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKS
RNQEVFHGSNLTSFNDSFGLPEDKNQASKEYKDLNSSENISKLSPDFDLA
TEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKIS
VTEDKILIEKENFNIEGNSDKWSCNQCSFKNDKTDLLCSLCYANNPYENT
SHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLD
RNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSCIKENNYRGYGTSTPV
NDISSNTSSRNEQDGKYSKNRIPIEEASNKITGKDSKLKQFHWYCINKKC
SAINFSMAKYCSKCFVKNECASSNSFFIVLKNDKEVKMCRNCESVYVTSY
CHCHKMMVTQV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001876Zinc finger, RanBP2-typeSMARTSM00547zf_4coord: 406..430
e-value: 0.24
score: 16.8
coord: 435..459
e-value: 0.059
score: 21.6
coord: 370..394
e-value: 0.4
score: 15.0
coord: 541..567
e-value: 5.7
score: 5.9
coord: 309..334
e-value: 1.1
score: 11.5
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 543..564
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 408..427
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 437..456
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 404..427
score: 8.005
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 368..397
score: 8.185