SMED30018337
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30018337 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30018337 aligns in the following genomic locations:
Homology
BLAST of SMED30018337 vs. Planmine SMEST
Match: SMESG000026858.1 (SMESG000026858.1) HSP 1 Score: 372.474 bits (955), Expect = 9.739e-120 Identity = 206/280 (73.57%), Postives = 229/280 (81.79%), Query Frame = 2 Query: 2066 MNSNSRSELNNLPRIPRLSPLVVALRQKXXXXXXXXXXXXTKYKRVKGTWTSPETAKGLHQKIVRLTRARDELITESRSQ*VLIKEQGTTLRQIFTDAELRASIPGSVWTRHLNIIPEEHLPMDTTNPTPSISAPRFTETPQVLLVE-----PLXXXXXXXXXXXXXXVEADLLREAELSNTQYRIDDLPPPYQEFEIPGFDPDVLYAPSVLFHSPARSTHIDLVEEESESPRIAQALDHLGLDLIEIAILTGNVPQISSPSEEAGSSTRIKVTYNINPP 2890 MN+NSRS+LN LPRIP LSP VVALRQKLEELE +Y L+ KY KGTWTSPE AKGLHQKI+ L+RARDELITESRSQ VLI+EQ TTLRQ+FTDA+LR IPGSVW RHLNIIPE+HLPMDT NPT S SAP+ E PQ+L+VE R R R R RR+EADLLRE E + YRIDDLPPPYQEF+IPG DPDVLYAPSVLFHSPARS HI+LV+EESESPR+ QALDHLGLDL EIA+LTG+VP+ S PSEEAG STRI+VTYN NPP Sbjct: 1 MNTNSRSDLNYLPRIPLLSPSVVALRQKLEELEGKYEALKAKYDWAKGTWTSPEAAKGLHQKIIWLSRARDELITESRSQRVLIEEQSTTLRQLFTDAKLRTRIPGSVWGRHLNIIPEQHLPMDTINPTSSTSAPQIMEPPQILVVEPRPRVARRRPNRNRRRRKSRRIEADLLRETEPPSAPYRIDDLPPPYQEFKIPGLDPDVLYAPSVLFHSPARSIHINLVDEESESPRVNQALDHLGLDLGEIAVLTGSVPRTSPPSEEAGPSTRIEVTYNTNPP 280
BLAST of SMED30018337 vs. Planmine SMEST
Match: SMESG000030584.1 (SMESG000030584.1) HSP 1 Score: 311.612 bits (797), Expect = 1.270e-97 Identity = 185/287 (64.46%), Postives = 217/287 (75.61%), Query Frame = 2 Query: 2084 SELNNLPRIPRLSPLVVALRQKXXXXXXXXXXXXTKYKRVKGTWTSPETAKGLHQKIVRLTRARDELITESRSQ*VLIKEQGTTLRQIFTDAELRASIPGSVWTRHLNIIPEEHLPMDTTNPTPSISAPRFTETPQVLLVEPLXXXX-----XXXXXXXXXXVEADLLREAELSNTQYRIDDLPPPYQEFEIPGFDPDVLYAPSVLFHSPARSTHIDLVEEESESPRIAQALDHLGLDLIEIAILTGNVPQISSPSEEAGSSTRIKVTYNINPPPYRPAKRRCLPTD 2929 ++LNNLPRIPRLSP VVALRQ LEELE++Y+ LRTKY+R+KGTWTSP AKGLHQKI+RL+RARDEL+TESRSQ VLIKEQ TL QIFT+AELRA IPGSVW RHLNIIPEEHLP++T PTPS S P+ TE+PQV +VEP A R R R RR+EADLLREAE S+ QYRIDDLPPPYQEFE+P FDPDVLYAP LF SP+ S HIDLV+++ E P QALD LGLD+ EIA+LT V + S E+G S R+ PP+ P+ + + D Sbjct: 2 TDLNNLPRIPRLSPSVVALRQWLEELEDKYKTLRTKYERMKGTWTSPVDAKGLHQKIIRLSRARDELVTESRSQWVLIKEQSRTLHQIFTNAELRARIPGSVWARHLNIIPEEHLPIETAIPTPSTSTPQVTESPQVPVVEPRTGAARRRPNRNRRRRESRRIEADLLREAEPSSAQYRIDDLPPPYQEFEMPEFDPDVLYAPIALFPSPSCSFHIDLVDDDYEVPVANQALDQLGLDIGEIAVLTSVVTKDPYRSNESGPSNRVD-------PPHPPSSQASVLAD 281
BLAST of SMED30018337 vs. Planmine SMEST
Match: SMESG000010758.1 (SMESG000010758.1) HSP 1 Score: 303.523 bits (776), Expect = 5.350e-94 Identity = 177/284 (62.32%), Postives = 217/284 (76.41%), Query Frame = 2 Query: 2105 RIPRLSPLVVALRQKXXXXXXXXXXXXTKYKRVKGTWTSPETAKGLHQKIVRLTRARDELITESRSQ*VLIKEQGTTLRQIFTDAELRASIPGSVWTRHLNIIPEEHLPMDTTNPTPSISAPRFTETPQVLLVEPLXXXXXXXXXXXXXX-----VEADLLREAELSNTQYRIDDLPPPYQEFEIPGFDPDVLYAPSVLFHSPARSTHIDLVEEESESPRIAQALDHLGLDLIEIAILTGNVPQISSPSEEAGSSTRIKVTYNINPPPYRPAKRRCLPTDIDAD 2941 +IPRLSP ++A+ QKLE+L+ + R KY R+KGTWTSPE K ++QKI RL+RARDELITESRSQ VLIKEQ TTLRQ FTDAELRA +PGS+W R+LNIIPE+HLP+D P PS AP+ E Q+ +VEP + ARRRPNRNRRR +EA+ LR E ++ YRIDDL PPYQEFE P DPDVLYAP+V FHSP RS HI+LV++ESE+P + ALD LGL++ EIA L GN P+ +E G STR++++ N+NP PYRPAKR+CLPTDIDAD Sbjct: 33 KIPRLSPSIIAICQKLEQLKRDHEAFRNKYHRLKGTWTSPEATKKMYQKITRLSRARDELITESRSQRVLIKEQSTTLRQKFTDAELRARVPGSIWARNLNIIPEKHLPLDVPRPAPSAPAPQSIEHSQIPVVEPHSGAARRRPNRNRRRRERRRMEANFLRTTEPASAPYRIDDLSPPYQEFEFPDLDPDVLYAPTVTFHSPPRSIHINLVDDESEAPALNNALDRLGLNVGEIAALMGNAPRSPPQIDEPGPSTRVELSINVNPHPYRPAKRQCLPTDIDAD 316
BLAST of SMED30018337 vs. Planmine SMEST
Match: SMESG000034473.1 (SMESG000034473.1) HSP 1 Score: 312.768 bits (800), Expect = 1.930e-91 Identity = 180/290 (62.07%), Postives = 211/290 (72.76%), Query Frame = 2 Query: 2087 ELNNLPRIPRLSPLVVALRQKXXXXXXXXXXXXTKYKRVKGTWTSPETAKGLHQKIVRLTRARDELITESRSQ*VLIKEQGTTLRQIFTDAELRASIPGSVWTRHLNIIPEEHLPMDTTNPTPSISAPRFTETPQVLLVEP-----LXXXXXXXXXXXXXXVEADLLREAELSNTQYRIDDLPPPYQEFEIPGFDPDVLYAPSVLFHSPARSTHIDLVEEESESPRIAQALDHLGLDLIEIAILTGNVPQISSPSEEAGSSTRIKVTYNINPPPYRPAKRRCLPTDIDAD 2941 E+ N+ + ++ALRQKLEELE R+ L+ KY RVKGTWTSPE K L+QKI L+RARDELITESRSQ VLIKEQ T LRQIFTDAELRA IPGSVW RHLNIIPE+H P++ NPTPS SAP Q+ + +P R R R R RRR+EA+LLRE E S+ YRIDDL PPYQEF+IPG DPDVLYAPS F+SPARS HI+LV++E+E + QAL+ LGLD+ +IA+LT NV E G STR + +N NPPPYRPAKRRCLPTDIDAD Sbjct: 540 EIKNVKERFKNDQRMIALRQKLEELEGRHEALKNKYNRVKGTWTSPEATKSLYQKISHLSRARDELITESRSQRVLIKEQSTMLRQIFTDAELRARIPGSVWARHLNIIPEQHFPLEAANPTPSTSAP------QIPIAKPRSGAARRRPNRNRRRRERRRMEAELLRETEPSSAPYRIDDLLPPYQEFKIPGLDPDVLYAPSATFYSPARSIHINLVDDENEVLAVNQALERLGLDIGKIAVLTSNVAGNPYRFNEPGPSTRTEPIHNANPPPYRPAKRRCLPTDIDAD 823
BLAST of SMED30018337 vs. Planmine SMEST
Match: SMESG000034473.1 (SMESG000034473.1) HSP 1 Score: 313.538 bits (802), Expect = 1.547e-89 Identity = 180/290 (62.07%), Postives = 211/290 (72.76%), Query Frame = 2 Query: 2087 ELNNLPRIPRLSPLVVALRQKXXXXXXXXXXXXTKYKRVKGTWTSPETAKGLHQKIVRLTRARDELITESRSQ*VLIKEQGTTLRQIFTDAELRASIPGSVWTRHLNIIPEEHLPMDTTNPTPSISAPRFTETPQVLLVEP-----LXXXXXXXXXXXXXXVEADLLREAELSNTQYRIDDLPPPYQEFEIPGFDPDVLYAPSVLFHSPARSTHIDLVEEESESPRIAQALDHLGLDLIEIAILTGNVPQISSPSEEAGSSTRIKVTYNINPPPYRPAKRRCLPTDIDAD 2941 E+ N+ + ++ALRQKLEELE R+ L+ KY RVKGTWTSPE K L+QKI L+RARDELITESRSQ VLIKEQ T LRQIFTDAELRA IPGSVW RHLNIIPE+H P++ NPTPS SAP Q+ + +P R R R R RRR+EA+LLRE E S+ YRIDDL PPYQEF+IPG DPDVLYAPS F+SPARS HI+LV++E+E + QAL+ LGLD+ +IA+LT NV E G STR + +N NPPPYRPAKRRCLPTDIDAD Sbjct: 848 EIKNVKERFKNDQRMIALRQKLEELEGRHEALKNKYNRVKGTWTSPEATKSLYQKISHLSRARDELITESRSQRVLIKEQSTMLRQIFTDAELRARIPGSVWARHLNIIPEQHFPLEAANPTPSTSAP------QIPIAKPRSGAARRRPNRNRRRRERRRMEAELLRETEPSSAPYRIDDLLPPYQEFKIPGLDPDVLYAPSATFYSPARSIHINLVDDENEVLAVNQALERLGLDIGKIAVLTSNVAGNPYRFNEPGPSTRTEPIHNANPPPYRPAKRRCLPTDIDAD 1131 The following BLAST results are available for this feature:
BLAST of SMED30018337 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30018337 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30018337 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30018337 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30018337 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30018337 ID=SMED30018337|Name=SMED30018337|organism=Schmidtea mediterranea sexual|type=transcript|length=3397bpback to top protein sequence of SMED30018337-orf-1 >SMED30018337-orf-1 ID=SMED30018337-orf-1|Name=SMED30018337-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=172bp MDTTNPTPSISAPRFTETPQVLLVEPLARTARRRPNRNRRRVEADLLREAback to top Annotated Terms
The following terms have been associated with this transcript:
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