Voltage-dependent T-type calcium channel subunit alpha

Overview
NameVoltage-dependent T-type calcium channel subunit alpha
Smed IDSMED30018324
Length (bp)6305
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Voltage-dependent T-type calcium channel subunit alpha (SMED30018324) t-SNE clustered cells

Violin plots show distribution of expression levels for Voltage-dependent T-type calcium channel subunit alpha (SMED30018324) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Voltage-dependent T-type calcium channel subunit alpha (SMED30018324) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Voltage-dependent T-type calcium channel subunit alpha (SMED30018324) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018324

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30018324h1SMcG0014350 SmedASXL_016738SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30018324h1SMcG0014350 Contig54903newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018324h1SMcG0014350 Contig54903uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30018324h1SMcG0014350 dd_Smed_v4_14730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018324h1SMcG0014350 dd_Smed_v4_14730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30018324h1SMcG0014350 dd_Smed_v4_14730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30018324h1SMcG0014350 dd_Smed_v4_14730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30018324h1SMcG0014350 dd_Smed_v4_14730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30018324h1SMcG0014350 dd_Smed_v4_14730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30018324h1SMcG0014350 dd_Smed_v6_14730_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1H (calcium voltage-gated channel subunit alpha1 H [Source:HGNC Symbol;Acc:HGNC:1395])

HSP 1 Score: 661.374 bits (1705), Expect = 0.000e+0
Identity = 397/618 (64.24%), Postives = 489/618 (79.13%), Query Frame = 1
Query: 3820 RYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIA--PIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            R   +LY+FS  N+FR  CQ ++  K FD++VL FI LNC+T+A+ERP I P S ER+FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+GP  + + TK  C + +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVGV+ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   S ED     C+S + A  P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D EL+ E++ ++ +
Sbjct:   11 REAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQC-RAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CSREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQ 622          

HSP 2 Score: 82.8037 bits (203), Expect = 6.484e-15
Identity = 60/234 (25.64%), Postives = 117/234 (50.00%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS 2772
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   S
Sbjct:  334 PYYADYSPTRRSIH-SLCTSHYLDLF--------ITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECS 558          

HSP 3 Score: 68.9366 bits (167), Expect = 1.003e-10
Identity = 61/282 (21.63%), Postives = 132/282 (46.81%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-------NNKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N   +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F+ EM++K++  G+L     Y+++ +NL DG++V++ ++++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E       + K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct:   22 QNRFRVSCQKV---ITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 300          

HSP 4 Score: 65.0846 bits (157), Expect = 1.616e-9
Identity = 33/112 (29.46%), Postives = 63/112 (56.25%), Query Frame = 1
Query:  328 ATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            A   ++F      R  C K+IT   F+ + +  I +NC+T+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+
Sbjct:   13 AWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERP--DIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIV 122          

HSP 5 Score: 54.6842 bits (130), Expect = 2.327e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct:  228 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 307          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1H (calcium voltage-gated channel subunit alpha1 H [Source:HGNC Symbol;Acc:HGNC:1395])

HSP 1 Score: 658.292 bits (1697), Expect = 0.000e+0
Identity = 411/676 (60.80%), Postives = 507/676 (75.00%), Query Frame = 1
Query: 3667 REKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIA--PIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAR 5685
            RE A E  DDS  E     R++ V +P     +   C S            R   +LY+FS  N+FR  CQ ++  K FD++VL FI LNC+T+A+ERP I P S ER+FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+GP  + + TK  C + +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVGV+ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   S ED     C+S + A  P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D EL+ E++ ++ +      R
Sbjct: 1236 REDAAELDDDS--EDSCCLRLHKVLEP----YKPQWCRS------------REAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQC-RAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CSREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQGPGSARR 1887          

HSP 2 Score: 372.474 bits (955), Expect = 2.988e-103
Identity = 237/451 (52.55%), Postives = 306/451 (67.85%), Query Frame = 1
Query:  313 YHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK-----TAQGNYQNKLKRQRK 1626
            Y    ATVF    Q   PR WCL+++  PWFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP          +++C+NWNQYY  C+ G+ NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE++ M  +R R  S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K       QG  Q    RQR+
Sbjct:   72 YPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYS-LDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPGRRELRMPCTLGWEAYTQPQAEGVGA--ARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRARHLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSAVQG--QGPGHRQRR 506          

HSP 3 Score: 337.806 bits (865), Expect = 1.792e-92
Identity = 189/320 (59.06%), Postives = 237/320 (74.06%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPY 3099
            K++ +V+   F RGI++AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF    +   T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   ++ +   K R         + Q+  L + + ++  +   E +  S  PP+I+ TAATPMPTP+SSP+
Sbjct:  785 KLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSVHFEEDFHKLR---------ELQTTELKMCSLAVTPNGHLEGRG-SLSPPLIMCTAATPMPTPKSSPF 1093          

HSP 4 Score: 112.464 bits (280), Expect = 9.012e-24
Identity = 75/280 (26.79%), Postives = 138/280 (49.29%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F   ++  I +N +++ +E    P    E L   L ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +     +   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  782 FSGKLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQP----EELTNALEISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIIVVISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKF----------------SLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1019          

HSP 5 Score: 82.0333 bits (201), Expect = 1.324e-14
Identity = 60/234 (25.64%), Postives = 117/234 (50.00%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS 2772
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   S
Sbjct: 1592 PYYADYSPTRRSIH-SLCTSHYLDLF--------ITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECS 1816          

HSP 6 Score: 80.1073 bits (196), Expect = 5.719e-14
Identity = 61/173 (35.26%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVVAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 72.7886 bits (177), Expect = 8.866e-12
Identity = 71/224 (31.70%), Postives = 117/224 (52.23%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W  +S K RR    +V SKYF   I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G   YI + +N  D +IV++S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  779 WVTFSGKLRR----IVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 984          

HSP 8 Score: 68.5514 bits (166), Expect = 1.922e-10
Identity = 61/282 (21.63%), Postives = 131/282 (46.45%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N   +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F+ EM++K++  G+L     Y+++ +NL DG++V++ ++++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1280 QNRFRVSCQKV---ITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1558          

HSP 9 Score: 65.4698 bits (158), Expect = 1.612e-9
Identity = 44/172 (25.58%), Postives = 81/172 (47.09%), Query Frame = 1
Query:  178 PQDNRSRRETLIGNKSDFNL-TDKSKADHCQQETDGTP---------INPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            P   R R   ++   SDF L  D  + D  + + D            + P +        A   ++F      R  C K+IT   F+ + +  I +NC+T+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+
Sbjct: 1211 PTKCRDRDGQVVALPSDFFLRIDSHREDAAELDDDSEDSCCLRLHKVLEPYKPQWCRSREAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERP--DIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIV 1380          

HSP 10 Score: 56.225 bits (134), Expect = 9.572e-7
Identity = 32/115 (27.83%), Postives = 60/115 (52.17%), Query Frame = 1
Query: 4399 PHLQAV-KTKNDCLKYSQ---------NEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            P  + V   +N C+ ++Q         +   N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  325 PQAEGVGAARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 429          

HSP 11 Score: 54.6842 bits (130), Expect = 2.902e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1486 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1565          

HSP 12 Score: 53.5286 bits (127), Expect = 5.829e-6
Identity = 35/132 (26.52%), Postives = 71/132 (53.79%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT----LEFSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            LV +  F+   ++ I++N +++G+    + V+        LE  +      F +EM++K++  G+ G   Y+ + +N  D  IVV G++E    G    LS +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:   96 LVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTL 227          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1H (calcium voltage-gated channel subunit alpha1 H [Source:HGNC Symbol;Acc:HGNC:1395])

HSP 1 Score: 658.292 bits (1697), Expect = 0.000e+0
Identity = 411/676 (60.80%), Postives = 507/676 (75.00%), Query Frame = 1
Query: 3667 REKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIA--PIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAR 5685
            RE A E  DDS  E     R++ V +P     +   C S            R   +LY+FS  N+FR  CQ ++  K FD++VL FI LNC+T+A+ERP I P S ER+FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+GP  + + TK  C + +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVGV+ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   S ED     C+S + A  P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D EL+ E++ ++ +      R
Sbjct: 1236 REDAAELDDDS--EDSCCLRLHKVLEP----YKPQWCRS------------REAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQC-RAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CSREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQGPGSARR 1887          

HSP 2 Score: 372.474 bits (955), Expect = 2.988e-103
Identity = 237/451 (52.55%), Postives = 306/451 (67.85%), Query Frame = 1
Query:  313 YHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK-----TAQGNYQNKLKRQRK 1626
            Y    ATVF    Q   PR WCL+++  PWFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP          +++C+NWNQYY  C+ G+ NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE++ M  +R R  S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K       QG  Q    RQR+
Sbjct:   72 YPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYS-LDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPGRRELRMPCTLGWEAYTQPQAEGVGA--ARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRARHLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSAVQG--QGPGHRQRR 506          

HSP 3 Score: 337.806 bits (865), Expect = 1.792e-92
Identity = 189/320 (59.06%), Postives = 237/320 (74.06%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPY 3099
            K++ +V+   F RGI++AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF    +   T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   ++ +   K R         + Q+  L + + ++  +   E +  S  PP+I+ TAATPMPTP+SSP+
Sbjct:  785 KLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSVHFEEDFHKLR---------ELQTTELKMCSLAVTPNGHLEGRG-SLSPPLIMCTAATPMPTPKSSPF 1093          

HSP 4 Score: 112.464 bits (280), Expect = 9.012e-24
Identity = 75/280 (26.79%), Postives = 138/280 (49.29%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F   ++  I +N +++ +E    P    E L   L ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +     +   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  782 FSGKLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQP----EELTNALEISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIIVVISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKF----------------SLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1019          

HSP 5 Score: 82.0333 bits (201), Expect = 1.324e-14
Identity = 60/234 (25.64%), Postives = 117/234 (50.00%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS 2772
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   S
Sbjct: 1592 PYYADYSPTRRSIH-SLCTSHYLDLF--------ITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECS 1816          

HSP 6 Score: 80.1073 bits (196), Expect = 5.719e-14
Identity = 61/173 (35.26%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVVAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 72.7886 bits (177), Expect = 8.866e-12
Identity = 71/224 (31.70%), Postives = 117/224 (52.23%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W  +S K RR    +V SKYF   I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G   YI + +N  D +IV++S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  779 WVTFSGKLRR----IVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 984          

HSP 8 Score: 68.5514 bits (166), Expect = 1.922e-10
Identity = 61/282 (21.63%), Postives = 131/282 (46.45%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N   +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F+ EM++K++  G+L     Y+++ +NL DG++V++ ++++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1280 QNRFRVSCQKV---ITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1558          

HSP 9 Score: 65.4698 bits (158), Expect = 1.612e-9
Identity = 44/172 (25.58%), Postives = 81/172 (47.09%), Query Frame = 1
Query:  178 PQDNRSRRETLIGNKSDFNL-TDKSKADHCQQETDGTP---------INPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            P   R R   ++   SDF L  D  + D  + + D            + P +        A   ++F      R  C K+IT   F+ + +  I +NC+T+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+
Sbjct: 1211 PTKCRDRDGQVVALPSDFFLRIDSHREDAAELDDDSEDSCCLRLHKVLEPYKPQWCRSREAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERP--DIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIV 1380          

HSP 10 Score: 56.225 bits (134), Expect = 9.572e-7
Identity = 32/115 (27.83%), Postives = 60/115 (52.17%), Query Frame = 1
Query: 4399 PHLQAV-KTKNDCLKYSQ---------NEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            P  + V   +N C+ ++Q         +   N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  325 PQAEGVGAARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 429          

HSP 11 Score: 54.6842 bits (130), Expect = 2.902e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1486 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1565          

HSP 12 Score: 53.5286 bits (127), Expect = 5.829e-6
Identity = 35/132 (26.52%), Postives = 71/132 (53.79%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT----LEFSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            LV +  F+   ++ I++N +++G+    + V+        LE  +      F +EM++K++  G+ G   Y+ + +N  D  IVV G++E    G    LS +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:   96 LVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTL 227          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1H (calcium voltage-gated channel subunit alpha1 H [Source:HGNC Symbol;Acc:HGNC:1395])

HSP 1 Score: 657.907 bits (1696), Expect = 0.000e+0
Identity = 398/629 (63.28%), Postives = 490/629 (77.90%), Query Frame = 1
Query: 3820 RYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPL-----------YWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIA--PIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            R   +LY+FS  N+FR  CQ ++  K FD++VL FI LNC+T+A+ERP I P S ER+FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+GP  + + TK  C + +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVGV+ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+    P+           Y+ +YS  RR+I+S+ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   S ED     C+S + A  P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D EL+ E++ ++ +
Sbjct:   11 REAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQC-RAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSKALPVAVAVAEAQRRPYYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CSREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQ 633          

HSP 2 Score: 87.4261 bits (215), Expect = 2.722e-16
Identity = 62/237 (26.16%), Postives = 119/237 (50.21%), Query Frame = 1
Query: 2092 HRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS 2772
             RRP+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   S
Sbjct:  342 QRRPYYADYSPTRRSIH-SLCTSHYLDLF--------ITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECS 569          

HSP 3 Score: 69.3218 bits (168), Expect = 9.685e-11
Identity = 61/282 (21.63%), Postives = 132/282 (46.81%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-------NNKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N   +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F+ EM++K++  G+L     Y+++ +NL DG++V++ ++++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E       + K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct:   22 QNRFRVSCQKV---ITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 300          

HSP 4 Score: 65.0846 bits (157), Expect = 1.470e-9
Identity = 33/112 (29.46%), Postives = 63/112 (56.25%), Query Frame = 1
Query:  328 ATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            A   ++F      R  C K+IT   F+ + +  I +NC+T+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+
Sbjct:   13 AWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERP--DIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIV 122          

HSP 5 Score: 54.6842 bits (130), Expect = 2.223e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct:  228 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 307          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Human
Match: CACNA1H (calcium voltage-gated channel subunit alpha1 H [Source:HGNC Symbol;Acc:HGNC:1395])

HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0
Identity = 399/625 (63.84%), Postives = 490/625 (78.40%), Query Frame = 1
Query: 3820 RYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHL-------RAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIA--PIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            R   +LY+FS  N+FR  CQ ++  K FD++VL FI LNC+T+A+ERP I P S ER+FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+GP  + + TK  C + +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVGV+ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+         R P Y+ +YS  RR+I+S+ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   S ED     C+S + A  P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D EL+ E++ ++ +
Sbjct:   11 REAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQC-RAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPSPEAQRRP-YYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CSREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQ 628          

HSP 2 Score: 87.8113 bits (216), Expect = 1.926e-16
Identity = 63/241 (26.14%), Postives = 120/241 (49.79%), Query Frame = 1
Query: 2080 SYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS 2772
            S    RRP+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   S
Sbjct:  333 SPEAQRRPYYADYSPTRRSIH-SLCTSHYLDLF--------ITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECS 564          

HSP 3 Score: 68.9366 bits (167), Expect = 9.881e-11
Identity = 61/282 (21.63%), Postives = 132/282 (46.81%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-------NNKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N   +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F+ EM++K++  G+L     Y+++ +NL DG++V++ ++++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E       + K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct:   22 QNRFRVSCQKV---ITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 300          

HSP 4 Score: 65.0846 bits (157), Expect = 1.514e-9
Identity = 33/112 (29.46%), Postives = 63/112 (56.25%), Query Frame = 1
Query:  328 ATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            A   ++F      R  C K+IT   F+ + +  I +NC+T+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+
Sbjct:   13 AWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERP--DIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIV 122          

HSP 5 Score: 54.6842 bits (130), Expect = 2.272e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct:  228 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 307          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Celegans
Match: cca-1 (Voltage-dependent T-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:A0A0K3ASP4])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 421/694 (60.66%), Postives = 506/694 (72.91%), Query Frame = 1
Query: 3664 AREKALEEPDD-----SNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILL-GHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE-----------NQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCD------DYELEREVQRDLDEEAKE 5676
            AREK LE   +     +  E +N ++ N               L K C         R   SL++    N  R KC     KK FDY VLFFI +NCITLAMERPSIPP S ER FL ++ YIFT+IFTGEM++KV++ G  IG  AY K GWNI+DG LV ISL+++   +L  G S +IFG++RV RLLR LRPLRVI+RAPG+KLVV TL+SSL+PIGNIVLICCTFFIIFGILGVQLFKG  Y+C GP +  V TK DC++  +N+W N +YNFDNLGQALM+LFVLSSKDGWV IMY GIDAVGV++QP EN+NEW +IYFISFLLLV FFVLNMFVGVVVENFHKC+E            +  + K +R         K++K  R   Y+ +Y   R  ++ +VTSKYFDLAIA VIG+NVI+MA EF+MMP  LK  LK  NYFFT+ F LEA +KL+ALG  R+  +KWN+LDM IVILSIAG++ +E E+  +PINPTI+RVMRVLRIARVLKLLKMA GIR+LLDTV +ALPQVGNLG LFFLLF+IFAALGVELFG+LECS+ +PC GLG HAHF+NFGMAFLTLFRIATGDNWNGIMKD LR+ C SS+ C+ NCCV  I+AP +FVIFVL++QFVLVNVVVAVLMKHLEES+K   +         +E E+ +  D+E  E
Sbjct: 1051 AREKFLEASQELKQALAEEEKRNEAKQNTF---------VRKLLKKTC------LHNRTEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSPKIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENFHKCKEALEKEMREKEKEKRLKRKLKRQKFEESMAGKRKKMERNYPYYHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECSEDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDALRDDCDSSDHCETNCCVDPILAPCFFVIFVLISQFVLVNVVVAVLMKHLEESNKRDAEGPAEPTGENIENEITKSDDDEIVE 1729          

HSP 2 Score: 375.941 bits (964), Expect = 3.504e-105
Identity = 217/406 (53.45%), Postives = 281/406 (69.21%), Query Frame = 1
Query:  307 LIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQ-NCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEIL--PSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRF---NSSNCS------SISKDEPQTCY 1488
            L Y GF       F+Q + PR W L+++  PWF++++M +I +NC+TL MY+PC D  +C+T +CQI+   D  IF +F  EMVIKI+A+GF G   Y  DTWNRLD FIV+ G  E +     G NI LTAIRT+RVLRPLRA+NRIPSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC +NL ++       L +  ++ R         ++ICS    NG+  CS++PP  +     +L + +        +  T  SC+NWN YY +CQ  ++NP++G+++FDNIGFAWVAIF VIS+E W DIMYYVQDAHSFW+WIYFV LI+IG+FFMINLCLVVIA+QF+ETKRRETERM  ER+     +S +C+      + SK+E  T Y
Sbjct:  100 LPYPGFAEPALRCFYQARPPRKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIFVYFAFEMVIKIMALGFYGPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAIRTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCVINLPKTISENQSALFNNVKLTRFYIPEDTSLEYICSQPDANGLHTCSNLPPYTVDGVKCNLTLDEY-------DKVTNDSCINWNIYYNECQVMQRNPFQGSVSFDNIGFAWVAIFLVISLEGWTDIMYYVQDAHSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRETERMLQERKMLLNRDSISCTGSEIGGASSKEEGDTVY 498          

HSP 3 Score: 300.056 bits (767), Expect = 1.593e-81
Identity = 166/281 (59.07%), Postives = 208/281 (74.02%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKR------------TCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHN--YRQSLKKRRANNKQLRIM 2955
            ++KI+  V    F RGIL+AIL+NTLSMG+EYHQQP  LT  LE+SN+ F+ LF +EM+LK+I  G+ GY+ +GFNLFDG IV L V+EL Q+G   GLS+LRTFRLLRILKLVRFMPALR QL VM+RT+DNV  FF LL+LFIFIFS LGM LFGCKFC   +                CDR NFD+ L++ VTVFQILTQEDWN VL+NGM +T+ WAALYF+ALMTFGNYVLFNLLVAILVEGF   + +E++ L     +Q++++     ++L ++
Sbjct:  643 REKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQEG-KGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKFCNHPDTGLQCTSAQVISSICECDRMNFDNFLFATVTVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARKQAVEEEDERKRELELI 922          

HSP 4 Score: 107.071 bits (266), Expect = 1.384e-22
Identity = 78/291 (26.80%), Postives = 138/291 (47.42%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPH-------LQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            FR+K Q  V    F   +L  I +N +++ +E    P      + L  +N  FT +F  EM++K+++ GL      YL  G+N+ DG +V +S+L+LF     G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF  LG+ LF  KF  C+ P         Q + +  +C           + NFDN   A + +F + +++ W  +++ G+             N WA +YF++ +    + + N+ V ++VE F + +E +
Sbjct:  642 FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQP--EILTVILEYSNLFFTALFALEMLLKIIASGLF----GYLADGFNLFDGGIVALSVLELFQEGKGGLS-----VLRTFRLLRILKLVRFM---PALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKF--CNHPDTGLQCTSAQVISSICEC----------DRMNFDNFLFATVTVFQILTQEDWNMVLFNGM----------AQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEE 896          

HSP 5 Score: 82.0333 bits (201), Expect = 5.222e-15
Identity = 61/175 (34.86%), Postives = 96/175 (54.86%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R+    +V    FD + +  I +NC+TL M RP    P     R  +   + N IF + F  EM+IK+++ G   G  AY+   WN +D F+V   + +  +   LG +  +  I    R +R LRPLR ++R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:  120 RKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIF-VYFAFEMVIKIMALGFY-GPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAI----RTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 288          

HSP 6 Score: 70.4774 bits (171), Expect = 1.575e-11
Identity = 93/326 (28.53%), Postives = 158/326 (48.47%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLG-------------RHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLI 5730
            L+W     +R  I   V   +F   I   I +N ++M  E+H  P++L   L+  N FFT+ F LE LLK++A GL  Y++D +N  D  IV LS+  +  +             + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C+  +P  GL                 +F+NF  A +T+F+I T ++WN ++ + + +              +   A +YFV  +    +VL N++VA+L++  +ES          + E +R L+E+A++ A E++  DE   E  LI
Sbjct:  639 LWW-----FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQE---------GKGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKFCN--HPDTGLQCTSAQVISSICECDRMNFDNFLFATVTVFQILTQEDWNMVLFNGMAQ--------------TNPWAALYFVALMTFGNYVLFNLLVAILVEGFQES----------KEEEKRQLEEDARKQAVEEE--DERKRELELI 922          

HSP 7 Score: 68.5514 bits (166), Expect = 7.162e-11
Identity = 54/211 (25.59%), Postives = 99/211 (46.92%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGM 2760
            +  +V    F   I   I IN +SM +E++  P+ L   L+  N  F+ +F +E  +KLI  G   +    +N  D  IV+L +  +  +           + + ++R  R+ R+LKL++    +R  L  +   +  V    +L  L  FIF+ LG+ LFG   C+       CD      +F +   + +T+F+I T ++WN ++ + +
Sbjct: 1441 LHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECS---EDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDAL 1648          

HSP 8 Score: 67.3958 bits (163), Expect = 1.313e-10
Identity = 69/278 (24.82%), Postives = 129/278 (46.40%), Query Frame = 1
Query: 2161 KIKTLVNDVK--FQRGILIAILINTLSMGIEYHQQPVQL--TQTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLL---------RILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFG---------------CKFCTIEN--NKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +IK L    K  F   +L  I IN +++ +E    P      Q L  S  +F+++F  EM++K+I  G       Y K+G+N+ DGI+VV+ +I +  +   +G S  + F ++         R L+++   P ++  +  +I ++  +     +   F  IF ILG+ LF                 K   IE+  NK    R NFD+L  +++++F + +++ W +++Y G+               W  +YFI+ +    + + N+ V ++VE F
Sbjct: 1108 RIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSP-KIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENF 1384          

HSP 9 Score: 65.4698 bits (158), Expect = 5.819e-10
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1
Query:  331 TVFHIFHQN-KHP-RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEI---LPSTGNN 678
            T F +F    K+P R  CL+     WF+   +  I +NCITL+M +P      ++ + Q +    YI    FT EM++K+IA G F+G   YF D WN LD  +V++  I I   L +TG++
Sbjct: 1094 TEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPP--DSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDS 1213          

HSP 10 Score: 64.3142 bits (155), Expect = 1.131e-9
Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 1
Query:  346 FHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSM---YQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGL----TAIRTIRVLRPLRAI 732
            +H   H R +   I+T  +F+     +I +N I+++M     P G +        +++A +Y   A FT E  +K+IA+GF     +F + WNRLDMFIVI+    I+      + L    T IR +RVLR  R +
Sbjct: 1431 YHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLK-------YVLKALNYFFTAVFTLEAAMKLIALGFKR---FFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVL 1556          

HSP 11 Score: 57.7658 bits (138), Expect = 1.081e-7
Identity = 25/81 (30.86%), Postives = 47/81 (58.02%), Query Frame = 1
Query: 1192 INFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            +NFDN  FA V +FQ+++ E W  +++      + W  +YFVAL+  G++ + NL + ++   F E+K  E  ++  + R+
Sbjct:  826 MNFDNFLFATVTVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARK 906          

HSP 12 Score: 54.299 bits (129), Expect = 1.260e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N+C    +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ AFF++N+ + V+   F + +  +
Sbjct:  377 NECQVMQRNPFQGS-VSFDNIGFAWVAIFLVISLEGWTDIMYYVQDA----------HSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRE 461          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Celegans
Match: cca-1 (Voltage-dependent T-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:A0A0K3ASP4])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 421/694 (60.66%), Postives = 506/694 (72.91%), Query Frame = 1
Query: 3664 AREKALEEPDD-----SNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILL-GHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE-----------NQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCD------DYELEREVQRDLDEEAKE 5676
            AREK LE   +     +  E +N ++ N               L K C         R   SL++    N  R KC     KK FDY VLFFI +NCITLAMERPSIPP S ER FL ++ YIFT+IFTGEM++KV++ G  IG  AY K GWNI+DG LV ISL+++   +L  G S +IFG++RV RLLR LRPLRVI+RAPG+KLVV TL+SSL+PIGNIVLICCTFFIIFGILGVQLFKG  Y+C GP +  V TK DC++  +N+W N +YNFDNLGQALM+LFVLSSKDGWV IMY GIDAVGV++QP EN+NEW +IYFISFLLLV FFVLNMFVGVVVENFHKC+E            +  + K +R         K++K  R   Y+ +Y   R  ++ +VTSKYFDLAIA VIG+NVI+MA EF+MMP  LK  LK  NYFFT+ F LEA +KL+ALG  R+  +KWN+LDM IVILSIAG++ +E E+  +PINPTI+RVMRVLRIARVLKLLKMA GIR+LLDTV +ALPQVGNLG LFFLLF+IFAALGVELFG+LECS+ +PC GLG HAHF+NFGMAFLTLFRIATGDNWNGIMKD LR+ C SS+ C+ NCCV  I+AP +FVIFVL++QFVLVNVVVAVLMKHLEES+K   +         +E E+ +  D+E  E
Sbjct: 1051 AREKFLEASQELKQALAEEEKRNEAKQNTF---------VRKLLKKTC------LHNRTEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSPKIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENFHKCKEALEKEMREKEKEKRLKRKLKRQKFEESMAGKRKKMERNYPYYHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECSEDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDALRDDCDSSDHCETNCCVDPILAPCFFVIFVLISQFVLVNVVVAVLMKHLEESNKRDAEGPAEPTGENIENEITKSDDDEIVE 1729          

HSP 2 Score: 375.941 bits (964), Expect = 3.504e-105
Identity = 217/406 (53.45%), Postives = 281/406 (69.21%), Query Frame = 1
Query:  307 LIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQ-NCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEIL--PSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRF---NSSNCS------SISKDEPQTCY 1488
            L Y GF       F+Q + PR W L+++  PWF++++M +I +NC+TL MY+PC D  +C+T +CQI+   D  IF +F  EMVIKI+A+GF G   Y  DTWNRLD FIV+ G  E +     G NI LTAIRT+RVLRPLRA+NRIPSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC +NL ++       L +  ++ R         ++ICS    NG+  CS++PP  +     +L + +        +  T  SC+NWN YY +CQ  ++NP++G+++FDNIGFAWVAIF VIS+E W DIMYYVQDAHSFW+WIYFV LI+IG+FFMINLCLVVIA+QF+ETKRRETERM  ER+     +S +C+      + SK+E  T Y
Sbjct:  100 LPYPGFAEPALRCFYQARPPRKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIFVYFAFEMVIKIMALGFYGPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAIRTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCVINLPKTISENQSALFNNVKLTRFYIPEDTSLEYICSQPDANGLHTCSNLPPYTVDGVKCNLTLDEY-------DKVTNDSCINWNIYYNECQVMQRNPFQGSVSFDNIGFAWVAIFLVISLEGWTDIMYYVQDAHSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRETERMLQERKMLLNRDSISCTGSEIGGASSKEEGDTVY 498          

HSP 3 Score: 300.056 bits (767), Expect = 1.593e-81
Identity = 166/281 (59.07%), Postives = 208/281 (74.02%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKR------------TCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHN--YRQSLKKRRANNKQLRIM 2955
            ++KI+  V    F RGIL+AIL+NTLSMG+EYHQQP  LT  LE+SN+ F+ LF +EM+LK+I  G+ GY+ +GFNLFDG IV L V+EL Q+G   GLS+LRTFRLLRILKLVRFMPALR QL VM+RT+DNV  FF LL+LFIFIFS LGM LFGCKFC   +                CDR NFD+ L++ VTVFQILTQEDWN VL+NGM +T+ WAALYF+ALMTFGNYVLFNLLVAILVEGF   + +E++ L     +Q++++     ++L ++
Sbjct:  643 REKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQEG-KGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKFCNHPDTGLQCTSAQVISSICECDRMNFDNFLFATVTVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARKQAVEEEDERKRELELI 922          

HSP 4 Score: 107.071 bits (266), Expect = 1.384e-22
Identity = 78/291 (26.80%), Postives = 138/291 (47.42%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPH-------LQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            FR+K Q  V    F   +L  I +N +++ +E    P      + L  +N  FT +F  EM++K+++ GL      YL  G+N+ DG +V +S+L+LF     G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF  LG+ LF  KF  C+ P         Q + +  +C           + NFDN   A + +F + +++ W  +++ G+             N WA +YF++ +    + + N+ V ++VE F + +E +
Sbjct:  642 FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQP--EILTVILEYSNLFFTALFALEMLLKIIASGLF----GYLADGFNLFDGGIVALSVLELFQEGKGGLS-----VLRTFRLLRILKLVRFM---PALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKF--CNHPDTGLQCTSAQVISSICEC----------DRMNFDNFLFATVTVFQILTQEDWNMVLFNGM----------AQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEE 896          

HSP 5 Score: 82.0333 bits (201), Expect = 5.222e-15
Identity = 61/175 (34.86%), Postives = 96/175 (54.86%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R+    +V    FD + +  I +NC+TL M RP    P     R  +   + N IF + F  EM+IK+++ G   G  AY+   WN +D F+V   + +  +   LG +  +  I    R +R LRPLR ++R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:  120 RKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIF-VYFAFEMVIKIMALGFY-GPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAI----RTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 288          

HSP 6 Score: 70.4774 bits (171), Expect = 1.575e-11
Identity = 93/326 (28.53%), Postives = 158/326 (48.47%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLG-------------RHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLI 5730
            L+W     +R  I   V   +F   I   I +N ++M  E+H  P++L   L+  N FFT+ F LE LLK++A GL  Y++D +N  D  IV LS+  +  +             + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C+  +P  GL                 +F+NF  A +T+F+I T ++WN ++ + + +              +   A +YFV  +    +VL N++VA+L++  +ES          + E +R L+E+A++ A E++  DE   E  LI
Sbjct:  639 LWW-----FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQE---------GKGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKFCN--HPDTGLQCTSAQVISSICECDRMNFDNFLFATVTVFQILTQEDWNMVLFNGMAQ--------------TNPWAALYFVALMTFGNYVLFNLLVAILVEGFQES----------KEEEKRQLEEDARKQAVEEE--DERKRELELI 922          

HSP 7 Score: 68.5514 bits (166), Expect = 7.162e-11
Identity = 54/211 (25.59%), Postives = 99/211 (46.92%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGM 2760
            +  +V    F   I   I IN +SM +E++  P+ L   L+  N  F+ +F +E  +KLI  G   +    +N  D  IV+L +  +  +           + + ++R  R+ R+LKL++    +R  L  +   +  V    +L  L  FIF+ LG+ LFG   C+       CD      +F +   + +T+F+I T ++WN ++ + +
Sbjct: 1441 LHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECS---EDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDAL 1648          

HSP 8 Score: 67.3958 bits (163), Expect = 1.313e-10
Identity = 69/278 (24.82%), Postives = 129/278 (46.40%), Query Frame = 1
Query: 2161 KIKTLVNDVK--FQRGILIAILINTLSMGIEYHQQPVQL--TQTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLL---------RILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFG---------------CKFCTIEN--NKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +IK L    K  F   +L  I IN +++ +E    P      Q L  S  +F+++F  EM++K+I  G       Y K+G+N+ DGI+VV+ +I +  +   +G S  + F ++         R L+++   P ++  +  +I ++  +     +   F  IF ILG+ LF                 K   IE+  NK    R NFD+L  +++++F + +++ W +++Y G+               W  +YFI+ +    + + N+ V ++VE F
Sbjct: 1108 RIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSP-KIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENF 1384          

HSP 9 Score: 65.4698 bits (158), Expect = 5.819e-10
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1
Query:  331 TVFHIFHQN-KHP-RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEI---LPSTGNN 678
            T F +F    K+P R  CL+     WF+   +  I +NCITL+M +P      ++ + Q +    YI    FT EM++K+IA G F+G   YF D WN LD  +V++  I I   L +TG++
Sbjct: 1094 TEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPP--DSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDS 1213          

HSP 10 Score: 64.3142 bits (155), Expect = 1.131e-9
Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 1
Query:  346 FHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSM---YQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGL----TAIRTIRVLRPLRAI 732
            +H   H R +   I+T  +F+     +I +N I+++M     P G +        +++A +Y   A FT E  +K+IA+GF     +F + WNRLDMFIVI+    I+      + L    T IR +RVLR  R +
Sbjct: 1431 YHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLK-------YVLKALNYFFTAVFTLEAAMKLIALGFKR---FFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVL 1556          

HSP 11 Score: 57.7658 bits (138), Expect = 1.081e-7
Identity = 25/81 (30.86%), Postives = 47/81 (58.02%), Query Frame = 1
Query: 1192 INFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            +NFDN  FA V +FQ+++ E W  +++      + W  +YFVAL+  G++ + NL + ++   F E+K  E  ++  + R+
Sbjct:  826 MNFDNFLFATVTVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARK 906          

HSP 12 Score: 54.299 bits (129), Expect = 1.260e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N+C    +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ AFF++N+ + V+   F + +  +
Sbjct:  377 NECQVMQRNPFQGS-VSFDNIGFAWVAIFLVISLEGWTDIMYYVQDA----------HSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRE 461          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Celegans
Match: cca-1 (Voltage-dependent T-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:A0A0K3ASP4])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 421/694 (60.66%), Postives = 506/694 (72.91%), Query Frame = 1
Query: 3664 AREKALEEPDD-----SNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILL-GHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE-----------NQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCD------DYELEREVQRDLDEEAKE 5676
            AREK LE   +     +  E +N ++ N               L K C         R   SL++    N  R KC     KK FDY VLFFI +NCITLAMERPSIPP S ER FL ++ YIFT+IFTGEM++KV++ G  IG  AY K GWNI+DG LV ISL+++   +L  G S +IFG++RV RLLR LRPLRVI+RAPG+KLVV TL+SSL+PIGNIVLICCTFFIIFGILGVQLFKG  Y+C GP +  V TK DC++  +N+W N +YNFDNLGQALM+LFVLSSKDGWV IMY GIDAVGV++QP EN+NEW +IYFISFLLLV FFVLNMFVGVVVENFHKC+E            +  + K +R         K++K  R   Y+ +Y   R  ++ +VTSKYFDLAIA VIG+NVI+MA EF+MMP  LK  LK  NYFFT+ F LEA +KL+ALG  R+  +KWN+LDM IVILSIAG++ +E E+  +PINPTI+RVMRVLRIARVLKLLKMA GIR+LLDTV +ALPQVGNLG LFFLLF+IFAALGVELFG+LECS+ +PC GLG HAHF+NFGMAFLTLFRIATGDNWNGIMKD LR+ C SS+ C+ NCCV  I+AP +FVIFVL++QFVLVNVVVAVLMKHLEES+K   +         +E E+ +  D+E  E
Sbjct: 1051 AREKFLEASQELKQALAEEEKRNEAKQNTF---------VRKLLKKTC------LHNRTEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSPKIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENFHKCKEALEKEMREKEKEKRLKRKLKRQKFEESMAGKRKKMERNYPYYHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECSEDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDALRDDCDSSDHCETNCCVDPILAPCFFVIFVLISQFVLVNVVVAVLMKHLEESNKRDAEGPAEPTGENIENEITKSDDDEIVE 1729          

HSP 2 Score: 375.941 bits (964), Expect = 3.504e-105
Identity = 217/406 (53.45%), Postives = 281/406 (69.21%), Query Frame = 1
Query:  307 LIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQ-NCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEIL--PSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRF---NSSNCS------SISKDEPQTCY 1488
            L Y GF       F+Q + PR W L+++  PWF++++M +I +NC+TL MY+PC D  +C+T +CQI+   D  IF +F  EMVIKI+A+GF G   Y  DTWNRLD FIV+ G  E +     G NI LTAIRT+RVLRPLRA+NRIPSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC +NL ++       L +  ++ R         ++ICS    NG+  CS++PP  +     +L + +        +  T  SC+NWN YY +CQ  ++NP++G+++FDNIGFAWVAIF VIS+E W DIMYYVQDAHSFW+WIYFV LI+IG+FFMINLCLVVIA+QF+ETKRRETERM  ER+     +S +C+      + SK+E  T Y
Sbjct:  100 LPYPGFAEPALRCFYQARPPRKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIFVYFAFEMVIKIMALGFYGPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAIRTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCVINLPKTISENQSALFNNVKLTRFYIPEDTSLEYICSQPDANGLHTCSNLPPYTVDGVKCNLTLDEY-------DKVTNDSCINWNIYYNECQVMQRNPFQGSVSFDNIGFAWVAIFLVISLEGWTDIMYYVQDAHSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRETERMLQERKMLLNRDSISCTGSEIGGASSKEEGDTVY 498          

HSP 3 Score: 300.056 bits (767), Expect = 1.593e-81
Identity = 166/281 (59.07%), Postives = 208/281 (74.02%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKR------------TCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHN--YRQSLKKRRANNKQLRIM 2955
            ++KI+  V    F RGIL+AIL+NTLSMG+EYHQQP  LT  LE+SN+ F+ LF +EM+LK+I  G+ GY+ +GFNLFDG IV L V+EL Q+G   GLS+LRTFRLLRILKLVRFMPALR QL VM+RT+DNV  FF LL+LFIFIFS LGM LFGCKFC   +                CDR NFD+ L++ VTVFQILTQEDWN VL+NGM +T+ WAALYF+ALMTFGNYVLFNLLVAILVEGF   + +E++ L     +Q++++     ++L ++
Sbjct:  643 REKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQEG-KGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKFCNHPDTGLQCTSAQVISSICECDRMNFDNFLFATVTVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARKQAVEEEDERKRELELI 922          

HSP 4 Score: 107.071 bits (266), Expect = 1.384e-22
Identity = 78/291 (26.80%), Postives = 138/291 (47.42%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPH-------LQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            FR+K Q  V    F   +L  I +N +++ +E    P      + L  +N  FT +F  EM++K+++ GL      YL  G+N+ DG +V +S+L+LF     G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF  LG+ LF  KF  C+ P         Q + +  +C           + NFDN   A + +F + +++ W  +++ G+             N WA +YF++ +    + + N+ V ++VE F + +E +
Sbjct:  642 FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQP--EILTVILEYSNLFFTALFALEMLLKIIASGLF----GYLADGFNLFDGGIVALSVLELFQEGKGGLS-----VLRTFRLLRILKLVRFM---PALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKF--CNHPDTGLQCTSAQVISSICEC----------DRMNFDNFLFATVTVFQILTQEDWNMVLFNGM----------AQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEE 896          

HSP 5 Score: 82.0333 bits (201), Expect = 5.222e-15
Identity = 61/175 (34.86%), Postives = 96/175 (54.86%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R+    +V    FD + +  I +NC+TL M RP    P     R  +   + N IF + F  EM+IK+++ G   G  AY+   WN +D F+V   + +  +   LG +  +  I    R +R LRPLR ++R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:  120 RKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIF-VYFAFEMVIKIMALGFY-GPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAI----RTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 288          

HSP 6 Score: 70.4774 bits (171), Expect = 1.575e-11
Identity = 93/326 (28.53%), Postives = 158/326 (48.47%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLG-------------RHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLI 5730
            L+W     +R  I   V   +F   I   I +N ++M  E+H  P++L   L+  N FFT+ F LE LLK++A GL  Y++D +N  D  IV LS+  +  +             + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C+  +P  GL                 +F+NF  A +T+F+I T ++WN ++ + + +              +   A +YFV  +    +VL N++VA+L++  +ES          + E +R L+E+A++ A E++  DE   E  LI
Sbjct:  639 LWW-----FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQE---------GKGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSTLGMVLFGCKFCN--HPDTGLQCTSAQVISSICECDRMNFDNFLFATVTVFQILTQEDWNMVLFNGMAQ--------------TNPWAALYFVALMTFGNYVLFNLLVAILVEGFQES----------KEEEKRQLEEDARKQAVEEE--DERKRELELI 922          

HSP 7 Score: 68.5514 bits (166), Expect = 7.162e-11
Identity = 54/211 (25.59%), Postives = 99/211 (46.92%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGM 2760
            +  +V    F   I   I IN +SM +E++  P+ L   L+  N  F+ +F +E  +KLI  G   +    +N  D  IV+L +  +  +           + + ++R  R+ R+LKL++    +R  L  +   +  V    +L  L  FIF+ LG+ LFG   C+       CD      +F +   + +T+F+I T ++WN ++ + +
Sbjct: 1441 LHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECS---EDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDAL 1648          

HSP 8 Score: 67.3958 bits (163), Expect = 1.313e-10
Identity = 69/278 (24.82%), Postives = 129/278 (46.40%), Query Frame = 1
Query: 2161 KIKTLVNDVK--FQRGILIAILINTLSMGIEYHQQPVQL--TQTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLL---------RILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFG---------------CKFCTIEN--NKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +IK L    K  F   +L  I IN +++ +E    P      Q L  S  +F+++F  EM++K+I  G       Y K+G+N+ DGI+VV+ +I +  +   +G S  + F ++         R L+++   P ++  +  +I ++  +     +   F  IF ILG+ LF                 K   IE+  NK    R NFD+L  +++++F + +++ W +++Y G+               W  +YFI+ +    + + N+ V ++VE F
Sbjct: 1108 RIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSP-KIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENF 1384          

HSP 9 Score: 65.4698 bits (158), Expect = 5.819e-10
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1
Query:  331 TVFHIFHQN-KHP-RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEI---LPSTGNN 678
            T F +F    K+P R  CL+     WF+   +  I +NCITL+M +P      ++ + Q +    YI    FT EM++K+IA G F+G   YF D WN LD  +V++  I I   L +TG++
Sbjct: 1094 TEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPP--DSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDS 1213          

HSP 10 Score: 64.3142 bits (155), Expect = 1.131e-9
Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 1
Query:  346 FHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSM---YQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGL----TAIRTIRVLRPLRAI 732
            +H   H R +   I+T  +F+     +I +N I+++M     P G +        +++A +Y   A FT E  +K+IA+GF     +F + WNRLDMFIVI+    I+      + L    T IR +RVLR  R +
Sbjct: 1431 YHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLK-------YVLKALNYFFTAVFTLEAAMKLIALGFKR---FFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVL 1556          

HSP 11 Score: 57.7658 bits (138), Expect = 1.081e-7
Identity = 25/81 (30.86%), Postives = 47/81 (58.02%), Query Frame = 1
Query: 1192 INFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            +NFDN  FA V +FQ+++ E W  +++      + W  +YFVAL+  G++ + NL + ++   F E+K  E  ++  + R+
Sbjct:  826 MNFDNFLFATVTVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARK 906          

HSP 12 Score: 54.299 bits (129), Expect = 1.260e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N+C    +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ AFF++N+ + V+   F + +  +
Sbjct:  377 NECQVMQRNPFQGS-VSFDNIGFAWVAIFLVISLEGWTDIMYYVQDA----------HSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRE 461          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Celegans
Match: cca-1 (Voltage-dependent T-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:A0A0K3ASP4])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 421/694 (60.66%), Postives = 506/694 (72.91%), Query Frame = 1
Query: 3664 AREKALEEPDD-----SNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILL-GHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE-----------NQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCD------DYELEREVQRDLDEEAKE 5676
            AREK LE   +     +  E +N ++ N               L K C         R   SL++    N  R KC     KK FDY VLFFI +NCITLAMERPSIPP S ER FL ++ YIFT+IFTGEM++KV++ G  IG  AY K GWNI+DG LV ISL+++   +L  G S +IFG++RV RLLR LRPLRVI+RAPG+KLVV TL+SSL+PIGNIVLICCTFFIIFGILGVQLFKG  Y+C GP +  V TK DC++  +N+W N +YNFDNLGQALM+LFVLSSKDGWV IMY GIDAVGV++QP EN+NEW +IYFISFLLLV FFVLNMFVGVVVENFHKC+E            +  + K +R         K++K  R   Y+ +Y   R  ++ +VTSKYFDLAIA VIG+NVI+MA EF+MMP  LK  LK  NYFFT+ F LEA +KL+ALG  R+  +KWN+LDM IVILSIAG++ +E E+  +PINPTI+RVMRVLRIARVLKLLKMA GIR+LLDTV +ALPQVGNLG LFFLLF+IFAALGVELFG+LECS+ +PC GLG HAHF+NFGMAFLTLFRIATGDNWNGIMKD LR+ C SS+ C+ NCCV  I+AP +FVIFVL++QFVLVNVVVAVLMKHLEES+K   +         +E E+ +  D+E  E
Sbjct: 1044 AREKFLEASQELKQALAEEEKRNEAKQNTF---------VRKLLKKTC------LHNRTEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSPKIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENFHKCKEALEKEMREKEKEKRLKRKLKRQKFEESMAGKRKKMERNYPYYHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECSEDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDALRDDCDSSDHCETNCCVDPILAPCFFVIFVLISQFVLVNVVVAVLMKHLEESNKRDAEGPAEPTGENIENEITKSDDDEIVE 1722          

HSP 2 Score: 375.941 bits (964), Expect = 3.372e-105
Identity = 217/406 (53.45%), Postives = 281/406 (69.21%), Query Frame = 1
Query:  307 LIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQ-NCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEIL--PSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRF---NSSNCS------SISKDEPQTCY 1488
            L Y GF       F+Q + PR W L+++  PWF++++M +I +NC+TL MY+PC D  +C+T +CQI+   D  IF +F  EMVIKI+A+GF G   Y  DTWNRLD FIV+ G  E +     G NI LTAIRT+RVLRPLRA+NRIPSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC +NL ++       L +  ++ R         ++ICS    NG+  CS++PP  +     +L + +        +  T  SC+NWN YY +CQ  ++NP++G+++FDNIGFAWVAIF VIS+E W DIMYYVQDAHSFW+WIYFV LI+IG+FFMINLCLVVIA+QF+ETKRRETERM  ER+     +S +C+      + SK+E  T Y
Sbjct:  100 LPYPGFAEPALRCFYQARPPRKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIFVYFAFEMVIKIMALGFYGPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAIRTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCVINLPKTISENQSALFNNVKLTRFYIPEDTSLEYICSQPDANGLHTCSNLPPYTVDGVKCNLTLDEY-------DKVTNDSCINWNIYYNECQVMQRNPFQGSVSFDNIGFAWVAIFLVISLEGWTDIMYYVQDAHSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRETERMLQERKMLLNRDSISCTGSEIGGASSKEEGDTVY 498          

HSP 3 Score: 315.464 bits (807), Expect = 2.176e-86
Identity = 169/274 (61.68%), Postives = 215/274 (78.47%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENN-----KRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHN--YRQSLKKRRANNKQLRIM 2955
            ++KI+  V    F RGIL+AIL+NTLSMG+EYHQQP  LT  LE+SN+ F+ LF +EM+LK+I  G+ GY+ +GFNLFDG IV L V+EL Q+G   GLS+LRTFRLLRILKLVRFMPALR QL VM+RT+DNV  FF LL+LFIFIFSILGM+LFGCKFC +E        + C+RKNFD+LLW+++TVFQILTQEDWN VL+NGM +T+ WAALYF+ALMTFGNYVLFNLLVAILVEGF   + +E++ L     +Q++++     ++L ++
Sbjct:  643 REKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQEG-KGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSILGMNLFGCKFCKVEEKFLGGLAKKCERKNFDTLLWALITVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARKQAVEEEDERKRELELI 915          

HSP 4 Score: 111.694 bits (278), Expect = 4.638e-24
Identity = 79/284 (27.82%), Postives = 138/284 (48.59%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            FR+K Q  V    F   +L  I +N +++ +E    P      + L  +N  FT +F  EM++K+++ GL      YL  G+N+ DG +V +S+L+LF     G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF  KF   +   L  +  K  C          ++ NFD L  AL+ +F + +++ W  +++ G+             N WA +YF++ +    + + N+ V ++VE F + +E +
Sbjct:  642 FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQP--EILTVILEYSNLFFTALFALEMLLKIIASGLF----GYLADGFNLFDGGIVALSVLELFQEGKGGLS-----VLRTFRLLRILKLVRFM---PALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSILGMNLFGCKFCKVEEKFLGGLAKK--C----------ERKNFDTLLWALITVFQILTQEDWNMVLFNGM----------AQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEE 889          

HSP 5 Score: 82.0333 bits (201), Expect = 5.771e-15
Identity = 61/175 (34.86%), Postives = 96/175 (54.86%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R+    +V    FD + +  I +NC+TL M RP    P     R  +   + N IF + F  EM+IK+++ G   G  AY+   WN +D F+V   + +  +   LG +  +  I    R +R LRPLR ++R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:  120 RKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIF-VYFAFEMVIKIMALGFY-GPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAI----RTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 288          

HSP 6 Score: 69.707 bits (169), Expect = 2.719e-11
Identity = 91/317 (28.71%), Postives = 155/317 (48.90%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCV-GLGRHAHFENFGM---AFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLI 5730
            L+W     +R  I   V   +F   I   I +N ++M  E+H  P++L   L+  N FFT+ F LE LLK++A GL  Y++D +N  D  IV LS+  +  +             + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C      + GL +    +NF     A +T+F+I T ++WN ++ + + +              +   A +YFV  +    +VL N++VA+L++  +ES          + E +R L+E+A++ A E++  DE   E  LI
Sbjct:  639 LWW-----FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQE---------GKGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSILGMNLFGCKFCKVEEKFLGGLAKKCERKNFDTLLWALITVFQILTQEDWNMVLFNGMAQ--------------TNPWAALYFVALMTFGNYVLFNLLVAILVEGFQES----------KEEEKRQLEEDARKQAVEEE--DERKRELELI 915          

HSP 7 Score: 68.1662 bits (165), Expect = 8.257e-11
Identity = 54/211 (25.59%), Postives = 99/211 (46.92%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGM 2760
            +  +V    F   I   I IN +SM +E++  P+ L   L+  N  F+ +F +E  +KLI  G   +    +N  D  IV+L +  +  +           + + ++R  R+ R+LKL++    +R  L  +   +  V    +L  L  FIF+ LG+ LFG   C+       CD      +F +   + +T+F+I T ++WN ++ + +
Sbjct: 1434 LHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECS---EDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDAL 1641          

HSP 8 Score: 67.3958 bits (163), Expect = 1.539e-10
Identity = 69/278 (24.82%), Postives = 129/278 (46.40%), Query Frame = 1
Query: 2161 KIKTLVNDVK--FQRGILIAILINTLSMGIEYHQQPVQL--TQTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLL---------RILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFG---------------CKFCTIEN--NKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +IK L    K  F   +L  I IN +++ +E    P      Q L  S  +F+++F  EM++K+I  G       Y K+G+N+ DGI+VV+ +I +  +   +G S  + F ++         R L+++   P ++  +  +I ++  +     +   F  IF ILG+ LF                 K   IE+  NK    R NFD+L  +++++F + +++ W +++Y G+               W  +YFI+ +    + + N+ V ++VE F
Sbjct: 1101 RIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSP-KIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENF 1377          

HSP 9 Score: 65.4698 bits (158), Expect = 5.576e-10
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1
Query:  331 TVFHIFHQN-KHP-RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEI---LPSTGNN 678
            T F +F    K+P R  CL+     WF+   +  I +NCITL+M +P    +  + + Q +    YI    FT EM++K+IA G F+G   YF D WN LD  +V++  I I   L +TG++
Sbjct: 1087 TEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPD--SFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDS 1206          

HSP 10 Score: 64.3142 bits (155), Expect = 1.112e-9
Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 1
Query:  346 FHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSM---YQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGL----TAIRTIRVLRPLRAI 732
            +H   H R +   I+T  +F+     +I +N I+++M     P G +        +++A +Y   A FT E  +K+IA+GF     +F + WNRLDMFIVI+    I+      + L    T IR +RVLR  R +
Sbjct: 1424 YHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLK-------YVLKALNYFFTAVFTLEAAMKLIALGFKR---FFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVL 1549          

HSP 11 Score: 54.299 bits (129), Expect = 1.441e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N+C    +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ AFF++N+ + V+   F + +  +
Sbjct:  377 NECQVMQRNPFQGS-VSFDNIGFAWVAIFLVISLEGWTDIMYYVQDA----------HSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRE 461          

HSP 12 Score: 53.5286 bits (127), Expect = 2.001e-6
Identity = 22/80 (27.50%), Postives = 46/80 (57.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            NFD + +A + +FQ+++ E W  +++      + W  +YFVAL+  G++ + NL + ++   F E+K  E  ++  + R+
Sbjct:  820 NFDTLLWALITVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARK 899          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Celegans
Match: cca-1 (Voltage-dependent T-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:A0A0K3ASP4])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 421/694 (60.66%), Postives = 506/694 (72.91%), Query Frame = 1
Query: 3664 AREKALEEPDD-----SNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILL-GHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE-----------NQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCD------DYELEREVQRDLDEEAKE 5676
            AREK LE   +     +  E +N ++ N               L K C         R   SL++    N  R KC     KK FDY VLFFI +NCITLAMERPSIPP S ER FL ++ YIFT+IFTGEM++KV++ G  IG  AY K GWNI+DG LV ISL+++   +L  G S +IFG++RV RLLR LRPLRVI+RAPG+KLVV TL+SSL+PIGNIVLICCTFFIIFGILGVQLFKG  Y+C GP +  V TK DC++  +N+W N +YNFDNLGQALM+LFVLSSKDGWV IMY GIDAVGV++QP EN+NEW +IYFISFLLLV FFVLNMFVGVVVENFHKC+E            +  + K +R         K++K  R   Y+ +Y   R  ++ +VTSKYFDLAIA VIG+NVI+MA EF+MMP  LK  LK  NYFFT+ F LEA +KL+ALG  R+  +KWN+LDM IVILSIAG++ +E E+  +PINPTI+RVMRVLRIARVLKLLKMA GIR+LLDTV +ALPQVGNLG LFFLLF+IFAALGVELFG+LECS+ +PC GLG HAHF+NFGMAFLTLFRIATGDNWNGIMKD LR+ C SS+ C+ NCCV  I+AP +FVIFVL++QFVLVNVVVAVLMKHLEES+K   +         +E E+ +  D+E  E
Sbjct: 1044 AREKFLEASQELKQALAEEEKRNEAKQNTF---------VRKLLKKTC------LHNRTEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSPKIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENFHKCKEALEKEMREKEKEKRLKRKLKRQKFEESMAGKRKKMERNYPYYHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECSEDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDALRDDCDSSDHCETNCCVDPILAPCFFVIFVLISQFVLVNVVVAVLMKHLEESNKRDAEGPAEPTGENIENEITKSDDDEIVE 1722          

HSP 2 Score: 375.941 bits (964), Expect = 3.372e-105
Identity = 217/406 (53.45%), Postives = 281/406 (69.21%), Query Frame = 1
Query:  307 LIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQ-NCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEIL--PSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRF---NSSNCS------SISKDEPQTCY 1488
            L Y GF       F+Q + PR W L+++  PWF++++M +I +NC+TL MY+PC D  +C+T +CQI+   D  IF +F  EMVIKI+A+GF G   Y  DTWNRLD FIV+ G  E +     G NI LTAIRT+RVLRPLRA+NRIPSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC +NL ++       L +  ++ R         ++ICS    NG+  CS++PP  +     +L + +        +  T  SC+NWN YY +CQ  ++NP++G+++FDNIGFAWVAIF VIS+E W DIMYYVQDAHSFW+WIYFV LI+IG+FFMINLCLVVIA+QF+ETKRRETERM  ER+     +S +C+      + SK+E  T Y
Sbjct:  100 LPYPGFAEPALRCFYQARPPRKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIFVYFAFEMVIKIMALGFYGPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAIRTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCVINLPKTISENQSALFNNVKLTRFYIPEDTSLEYICSQPDANGLHTCSNLPPYTVDGVKCNLTLDEY-------DKVTNDSCINWNIYYNECQVMQRNPFQGSVSFDNIGFAWVAIFLVISLEGWTDIMYYVQDAHSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRETERMLQERKMLLNRDSISCTGSEIGGASSKEEGDTVY 498          

HSP 3 Score: 315.464 bits (807), Expect = 2.176e-86
Identity = 169/274 (61.68%), Postives = 215/274 (78.47%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENN-----KRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHN--YRQSLKKRRANNKQLRIM 2955
            ++KI+  V    F RGIL+AIL+NTLSMG+EYHQQP  LT  LE+SN+ F+ LF +EM+LK+I  G+ GY+ +GFNLFDG IV L V+EL Q+G   GLS+LRTFRLLRILKLVRFMPALR QL VM+RT+DNV  FF LL+LFIFIFSILGM+LFGCKFC +E        + C+RKNFD+LLW+++TVFQILTQEDWN VL+NGM +T+ WAALYF+ALMTFGNYVLFNLLVAILVEGF   + +E++ L     +Q++++     ++L ++
Sbjct:  643 REKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQEG-KGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSILGMNLFGCKFCKVEEKFLGGLAKKCERKNFDTLLWALITVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARKQAVEEEDERKRELELI 915          

HSP 4 Score: 111.694 bits (278), Expect = 4.638e-24
Identity = 79/284 (27.82%), Postives = 138/284 (48.59%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            FR+K Q  V    F   +L  I +N +++ +E    P      + L  +N  FT +F  EM++K+++ GL      YL  G+N+ DG +V +S+L+LF     G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF  KF   +   L  +  K  C          ++ NFD L  AL+ +F + +++ W  +++ G+             N WA +YF++ +    + + N+ V ++VE F + +E +
Sbjct:  642 FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQP--EILTVILEYSNLFFTALFALEMLLKIIASGLF----GYLADGFNLFDGGIVALSVLELFQEGKGGLS-----VLRTFRLLRILKLVRFM---PALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSILGMNLFGCKFCKVEEKFLGGLAKK--C----------ERKNFDTLLWALITVFQILTQEDWNMVLFNGM----------AQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEE 889          

HSP 5 Score: 82.0333 bits (201), Expect = 5.771e-15
Identity = 61/175 (34.86%), Postives = 96/175 (54.86%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R+    +V    FD + +  I +NC+TL M RP    P     R  +   + N IF + F  EM+IK+++ G   G  AY+   WN +D F+V   + +  +   LG +  +  I    R +R LRPLR ++R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:  120 RKWALQMVMSPWFDRITMAVIMINCVTLGMYRPCEDGPDCDTYRCQILDIIDNCIF-VYFAFEMVIKIMALGFY-GPAAYMSDTWNRLDFFIVMAGIAEFVLHEYLGGNINLTAI----RTVRVLRPLRAVNRIPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 288          

HSP 6 Score: 69.707 bits (169), Expect = 2.719e-11
Identity = 91/317 (28.71%), Postives = 155/317 (48.90%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCV-GLGRHAHFENFGM---AFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLI 5730
            L+W     +R  I   V   +F   I   I +N ++M  E+H  P++L   L+  N FFT+ F LE LLK++A GL  Y++D +N  D  IV LS+  +  +             + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C      + GL +    +NF     A +T+F+I T ++WN ++ + + +              +   A +YFV  +    +VL N++VA+L++  +ES          + E +R L+E+A++ A E++  DE   E  LI
Sbjct:  639 LWW-----FREKIQKFVICDHFTRGILVAILVNTLSMGVEYHQQPEILTVILEYSNLFFTALFALEMLLKIIASGLFGYLADGFNLFDGGIVALSVLELFQE---------GKGGLSVLRTFRLLRILKLVRFMPALRYQLVVMLRTMDNVTVFFGLLVLFIFIFSILGMNLFGCKFCKVEEKFLGGLAKKCERKNFDTLLWALITVFQILTQEDWNMVLFNGMAQ--------------TNPWAALYFVALMTFGNYVLFNLLVAILVEGFQES----------KEEEKRQLEEDARKQAVEEE--DERKRELELI 915          

HSP 7 Score: 68.1662 bits (165), Expect = 8.257e-11
Identity = 54/211 (25.59%), Postives = 99/211 (46.92%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGM 2760
            +  +V    F   I   I IN +SM +E++  P+ L   L+  N  F+ +F +E  +KLI  G   +    +N  D  IV+L +  +  +           + + ++R  R+ R+LKL++    +R  L  +   +  V    +L  L  FIF+ LG+ LFG   C+       CD      +F +   + +T+F+I T ++WN ++ + +
Sbjct: 1434 LHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLKYVLKALNYFFTAVFTLEAAMKLIALGFKRFFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVLKLLKMAKGIRSLLDTVGEALPQVGNLGSLFFLLFFIFAALGVELFGKLECS---EDHPCDGLGEHAHFKNFGMAFLTLFRIATGDNWNGIMKDAL 1641          

HSP 8 Score: 67.3958 bits (163), Expect = 1.539e-10
Identity = 69/278 (24.82%), Postives = 129/278 (46.40%), Query Frame = 1
Query: 2161 KIKTLVNDVK--FQRGILIAILINTLSMGIEYHQQPVQL--TQTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLL---------RILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFG---------------CKFCTIEN--NKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +IK L    K  F   +L  I IN +++ +E    P      Q L  S  +F+++F  EM++K+I  G       Y K+G+N+ DGI+VV+ +I +  +   +G S  + F ++         R L+++   P ++  +  +I ++  +     +   F  IF ILG+ LF                 K   IE+  NK    R NFD+L  +++++F + +++ W +++Y G+               W  +YFI+ +    + + N+ V ++VE F
Sbjct: 1101 RIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPDSFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDSP-KIFGVIRVLRLLRALRPLRVINRAPGVKLVVMTLISSLKPIGNIVLICCTFFIIFGILGVQLFKGMMYHCIGPEVGNVTTKADCIEDYRNKWVNHRYNFDNLGQALMSLFVLSSKDGWVSIMYQGIDAVGVDVQPIENYNEWRMIYFISFLLLVGFFVLNMFVGVVVENF 1377          

HSP 9 Score: 65.4698 bits (158), Expect = 5.576e-10
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 1
Query:  331 TVFHIFHQN-KHP-RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEI---LPSTGNN 678
            T F +F    K+P R  CL+     WF+   +  I +NCITL+M +P    +  + + Q +    YI    FT EM++K+IA G F+G   YF D WN LD  +V++  I I   L +TG++
Sbjct: 1087 TEFSLFLMGPKNPLRIKCLQTTQKKWFDYTVLFFIGINCITLAMERPSIPPD--SFERQFLHISGYIFTVIFTGEMMMKVIANGCFIGQAAYFKDGWNILDGILVVISLINIAFELLATGDS 1206          

HSP 10 Score: 64.3142 bits (155), Expect = 1.112e-9
Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 1
Query:  346 FHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSM---YQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGL----TAIRTIRVLRPLRAI 732
            +H   H R +   I+T  +F+     +I +N I+++M     P G +        +++A +Y   A FT E  +K+IA+GF     +F + WNRLDMFIVI+    I+      + L    T IR +RVLR  R +
Sbjct: 1424 YHDYGHTRLFLHGIVTSKYFDLAIAAVIGINVISMAMEFYMMPMGLK-------YVLKALNYFFTAVFTLEAAMKLIALGFKR---FFIEKWNRLDMFIVILSIAGIIFEEFEALELPINPTIIRVMRVLRIARVL 1549          

HSP 11 Score: 54.299 bits (129), Expect = 1.441e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N+C    +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ AFF++N+ + V+   F + +  +
Sbjct:  377 NECQVMQRNPFQGS-VSFDNIGFAWVAIFLVISLEGWTDIMYYVQDA----------HSFWNWIYFVLLIVIGAFFMINLCLVVIATQFAETKRRE 461          

HSP 12 Score: 53.5286 bits (127), Expect = 2.001e-6
Identity = 22/80 (27.50%), Postives = 46/80 (57.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            NFD + +A + +FQ+++ E W  +++      + W  +YFVAL+  G++ + NL + ++   F E+K  E  ++  + R+
Sbjct:  820 NFDTLLWALITVFQILTQEDWNMVLFNGMAQTNPWAALYFVALMTFGNYVLFNLLVAILVEGFQESKEEEKRQLEEDARK 899          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Fly
Match: Ca-alpha1T (gene:FBgn0264386 transcript:FBtr0304609)

HSP 1 Score: 784.637 bits (2025), Expect = 0.000e+0
Identity = 446/636 (70.13%), Postives = 534/636 (83.96%), Query Frame = 1
Query: 3799 PKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN---FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQL 5697
            PK    +R  +SLY+F  DN+FRQ C   V++K FD +VL FI LNCITLAMERP+IPP S ERLFL   NY+FT++FT EM IKV++ G+  G  AY  SGWNIMDG LV IS++DL ++++   S RIFGILRVFRLLR+LRPLRVI+RAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC+G +++ V+  ++C +   N W N+KYNFD+LG+ALM+LFVLSS+DGWV IMYTG+DAVGV+ QP  N+NEW L+YFI+F+LLV FFVLNMFVGVVVENFH+C+E QEKEEK RRAAKRA ++EKKR+ +  P Y+ NYS  R  +++VVTSKYFDLAIA VIGLNV+TMA E++ MP  LK ALK+FNYFFT+ F+LEA +KLVALG   Y+ D+WNQLD+ IV+LSI G+V++E+E+N    +PINPTI+RVMRVLRIARVLKLLKMA+GIRALLDTV+QALPQVGNLGLLFFLLF+IFAALGVELFGRLECSD  PC GLG HAHF NFGMAFLTLFR+ATGDNWNGIMKDTLR+ C  + DC RNCCVS++IAPI+FVIFVLMAQFVLVNVVVAVLMKHLEESHK M D+ ++E E++R+L  E +E A+E++L
Sbjct: 2105 PKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDMEVELERELVRE-QEFAQEQKL 2739          

HSP 2 Score: 417.157 bits (1071), Expect = 3.580e-117
Identity = 249/435 (57.24%), Postives = 308/435 (70.80%), Query Frame = 1
Query:  295 PNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKD--EPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQG 1593
            PN  L Y GF         Q   PR+WCL +IT PWFE++S+ +I +NCITL MYQPC D  C T++C+I+Q FD IIFAFF  EM IK++AMG  G   Y  D+WNRLD FIV+ G +E +     N+ LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W+G+LR RC L   E   +P L LS +Y+ ++       D+D+ICST  ++G+  C + PP  +     SL +C + A+ F  N PT  SCVNWNQYYT C+   +NP++G I+FDNIG AWVAIF VIS+E W DIMYYVQDAHSFWDWIYFV LI+IGSFFMINLCLVVIA+QFSETK+RE ERM  ER R+ S++  + S +  EP TCY EI+K I  +  +  RR++K Y   + +  +  +G
Sbjct:  269 PN--LPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHV-ENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYP-LTLSQYYEFSK-------DQDYICSTPNDSGMHLCGNFPPYRI----GSL-VCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEG 687          

HSP 3 Score: 322.783 bits (826), Expect = 4.327e-88
Identity = 181/272 (66.54%), Postives = 212/272 (77.94%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQ-------QGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS--------------ERLDEQKLLHNYRQSL 2916
            I+ LV    FQ+GIL+AILINTLSMGIEYH QP +LT  +E SN+VFS +F +EM+LK++ EG   YI NGFN+FDGIIV+L  IE+CQ        GG SGLS+LRTFRLLRILKLVRFMP LR+QLFVM+RT+DNVA FF+LL+LFIFIFSILGM+LFGCKFC   N +  CDRKNFDSLLW++VTVFQILTQEDWN VL+NGM +TS WAALYF+ALMTFGNYVLFNLLVAILVEGFSS              ++L E+ L  NY   +
Sbjct: 1216 IRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKFCEKVNGEMICDRKNFDSLLWALVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKKLREETLAENYSDGM 1487          

HSP 4 Score: 107.457 bits (267), Expect = 1.266e-22
Identity = 87/343 (25.36%), Postives = 159/343 (46.36%), Query Frame = 1
Query: 3706 ESQNSSRMNNVEDPDEEDMEAMIC--------LSKCCPQPKGIF-AKRYYHSLY--VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDL---FITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            + Q +SRM     P    ME   C        LS    +P+      R   S+Y  +  V +  R+  + +V+ K F   +L  I +N +++ +E  + PP       +  +N +F+ IF  EM++KV+++G       Y+ +G+N+ DG +V +S +++   F+    G       +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF  KF                C K +  E    + NFD+L  AL+ +F + +++ W  +++ G+          E  + WA +YF++ +    + + N+ V ++VE F
Sbjct: 1149 QGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTA--IVETSNVVFSGIFAVEMLLKVVAEGPF----RYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFM---PNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKF----------------CEKVN-GEMICDRKNFDSLLWALVTVFQILTQEDWNVVLFNGM----------EKTSHWAALYFVALMTFGNYVLFNLLVAILVEGF 1455          

HSP 5 Score: 82.0333 bits (201), Expect = 6.864e-15
Identity = 80/308 (25.97%), Postives = 139/308 (45.13%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTL--EFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILK---------LVRFMPALR---QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTCDR----------KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQH 2961
             VN   F   +L+ I +N +++ +E    P   T+ L    +N VF+++F +EM +K++  G+      Y  +G+N+ DG +V + +I+L     +   S  R F +LR+ +         ++   P L+   Q L   +R I N+     +   F  IF ILG+ LF   F  C  EN     N   C R           NFD L  +++++F + +++ W  ++Y G+               W  LYFIA +    + + N+ V ++VE F   R +EQ+     R++ K+     K+ R M  
Sbjct: 2133 FVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISE--SSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIV---LICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCR-EEQEKEEKIRRAAKRALQMEKKRRRMHE 2434          

HSP 6 Score: 81.2629 bits (199), Expect = 1.355e-14
Identity = 60/173 (34.68%), Postives = 93/173 (53.76%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  ++    F+ + +  I LNCITL M +P +     +     L + + I    F  EM IK+++ G+  G   YL   WN +D F+V   LL+  +     H   +   L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL++G
Sbjct:  291 RNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGI-CGKNTYLADSWNRLDFFIVLAGLLEYVM-----HVENLN--LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEG 455          

HSP 7 Score: 72.4034 bits (176), Expect = 5.446e-12
Identity = 52/207 (25.12%), Postives = 104/207 (50.24%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-----------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-NNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            +  +V    F   I   I +N ++M +EY++ P  L   L+  N  F+ +F++E  +KL+  G   Y+K+ +N  D  IV+L ++ +  +   +            + ++R  R+ R+LKL++    +R  L  +++ +  V     L  L  FIF+ LG+ LFG   C+ E   +   +  +F +   + +T+F++ T ++WN ++
Sbjct: 2448 VHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIM 2654          

HSP 8 Score: 71.2478 bits (173), Expect = 1.209e-11
Identity = 68/211 (32.23%), Postives = 110/211 (52.13%), Query Frame = 1
Query: 4819 RRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFM----PINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIM 5439
            RR I  +V  KYF   I   I +N ++M  E+H  P  L   ++  N  F+  F +E LLK+VA G  RYI++ +N  D +IVILS       E+   FM        + + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C  +N  +   R  +F++   A +T+F+I T ++WN ++
Sbjct: 1213 RRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAI-----EICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKFCEKVNGEMICDRK-NFDSLLWALVTVFQILTQEDWNVVL 1417          

HSP 9 Score: 67.0106 bits (162), Expect = 2.247e-10
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQ--IMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPL---------RAINRIPSMRI 756
             +IF ++   R  C   +   WF+ + +  IA+NCITL+M +P    N   S  +   +   +Y+    FT EM IK++A G F G   YF   WN +D  +V +  I++L S    I  ++ R   +LR           R INR P +++
Sbjct: 2117 LYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERP----NIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSL---ISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKL 2261          

HSP 10 Score: 55.8398 bits (133), Expect = 5.436e-7
Identity = 37/136 (27.21%), Postives = 75/136 (55.15%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQP-------VQLTQTLE-FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            L+ +  F+R  ++ IL+N +++G+    QP           + L+ F +I+F+  F +EM +K++  GI G   Y+ + +N  D  IV+ G++E         L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:  297 LITNPWFERVSILVILLNCITLGM---YQPCVDDACVTNRCKILQIFDDIIFA-FFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTL 428          

HSP 11 Score: 54.6842 bits (130), Expect = 1.313e-6
Identity = 27/94 (28.72%), Postives = 53/94 (56.38%), Query Frame = 1
Query: 4432 CLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            C +  +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ +FF++N+ + V+   F + ++ +
Sbjct:  540 CKQSGENPFQG-TISFDNIGMAWVAIFLVISLEGWTDIMYYVQDA----------HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKRE 622          

HSP 12 Score: 54.6842 bits (130), Expect = 1.369e-6
Identity = 21/64 (32.81%), Postives = 40/64 (62.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFS 1386
            NFD++ +A V +FQ+++ E W  +++   +  S W  +YFVAL+  G++ + NL + ++   FS
Sbjct: 1393 NFDSLLWALVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFS 1456          

HSP 13 Score: 53.9138 bits (128), Expect = 2.548e-6
Identity = 26/88 (29.55%), Postives = 48/88 (54.55%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIER 1428
            NFD++G A +++F + S + WV+IMY   DA          ++ W  +YF+A I++  FF++N+ + V+   F   +  + +   I R
Sbjct: 2330 NFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRR 2417          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Fly
Match: Ca-alpha1T (gene:FBgn0264386 transcript:FBtr0112953)

HSP 1 Score: 784.637 bits (2025), Expect = 0.000e+0
Identity = 446/636 (70.13%), Postives = 534/636 (83.96%), Query Frame = 1
Query: 3799 PKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN---FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQL 5697
            PK    +R  +SLY+F  DN+FRQ C   V++K FD +VL FI LNCITLAMERP+IPP S ERLFL   NY+FT++FT EM IKV++ G+  G  AY  SGWNIMDG LV IS++DL ++++   S RIFGILRVFRLLR+LRPLRVI+RAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC+G +++ V+  ++C +   N W N+KYNFD+LG+ALM+LFVLSS+DGWV IMYTG+DAVGV+ QP  N+NEW L+YFI+F+LLV FFVLNMFVGVVVENFH+C+E QEKEEK RRAAKRA ++EKKR+ +  P Y+ NYS  R  +++VVTSKYFDLAIA VIGLNV+TMA E++ MP  LK ALK+FNYFFT+ F+LEA +KLVALG   Y+ D+WNQLD+ IV+LSI G+V++E+E+N    +PINPTI+RVMRVLRIARVLKLLKMA+GIRALLDTV+QALPQVGNLGLLFFLLF+IFAALGVELFGRLECSD  PC GLG HAHF NFGMAFLTLFR+ATGDNWNGIMKDTLR+ C  + DC RNCCVS++IAPI+FVIFVLMAQFVLVNVVVAVLMKHLEESHK M D+ ++E E++R+L  E +E A+E++L
Sbjct: 2130 PKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDMEVELERELVRE-QEFAQEQKL 2764          

HSP 2 Score: 417.157 bits (1071), Expect = 4.045e-117
Identity = 249/435 (57.24%), Postives = 308/435 (70.80%), Query Frame = 1
Query:  295 PNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKD--EPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQG 1593
            PN  L Y GF         Q   PR+WCL +IT PWFE++S+ +I +NCITL MYQPC D  C T++C+I+Q FD IIFAFF  EM IK++AMG  G   Y  D+WNRLD FIV+ G +E +     N+ LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W+G+LR RC L   E   +P L LS +Y+ ++       D+D+ICST  ++G+  C + PP  +     SL +C + A+ F  N PT  SCVNWNQYYT C+   +NP++G I+FDNIG AWVAIF VIS+E W DIMYYVQDAHSFWDWIYFV LI+IGSFFMINLCLVVIA+QFSETK+RE ERM  ER R+ S++  + S +  EP TCY EI+K I  +  +  RR++K Y   + +  +  +G
Sbjct:  269 PN--LPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHV-ENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYP-LTLSQYYEFSK-------DQDYICSTPNDSGMHLCGNFPPYRI----GSL-VCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEG 687          

HSP 3 Score: 298.516 bits (763), Expect = 1.342e-80
Identity = 175/289 (60.55%), Postives = 209/289 (72.32%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQ-------QGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTI-----------------ENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS--------------ERLDEQKLLHNYRQSL 2916
            I+ LV    FQ+GIL+AILINTLSMGIEYH QP +LT  +E SN+VFS +F +EM+LK++ EG   YI NGFN+FDGIIV+L  IE+CQ        GG SGLS+LRTFRLLRILKLVRFMP LR+QLFVM+RT+DNVA FF+LL+LFIFIFSILGM LFG KFC                    + +  CDRK+F+++LW+ VTVFQILTQEDWN VL+NGM +TS WAALYF+ALMTFGNYVLFNLLVAILVEGFSS              ++L E+ L  NY   +
Sbjct: 1224 IRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKKLREETLAENYSDGM 1512          

HSP 4 Score: 107.842 bits (268), Expect = 1.216e-22
Identity = 87/345 (25.22%), Postives = 166/345 (48.12%), Query Frame = 1
Query: 3706 ESQNSSRMNNVEDPDEEDMEAMIC--------LSKCCPQPKGIF-AKRYYHSLY--VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDL---FITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF-YYCDGPHLQAVKTKNDCL-KYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            + Q +SRM     P    ME   C        LS    +P+      R   S+Y  +  V +  R+  + +V+ K F   +L  I +N +++ +E  + PP       +  +N +F+ IF  EM++KV+++G       Y+ +G+N+ DG +V +S +++   F+    G       +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF GKF  + D   L+   T  + + ++ Q E    + +F+N+  A + +F + +++ W  +++ G+          E  + WA +YF++ +    + + N+ V ++VE F
Sbjct: 1157 QGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTA--IVETSNVVFSGIFAVEMLLKVVAEGPF----RYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFM---PNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCEC--DRKHFNNILWATVTVFQILTQEDWNVVLFNGM----------EKTSHWAALYFVALMTFGNYVLFNLLVAILVEGF 1480          

HSP 5 Score: 82.0333 bits (201), Expect = 6.756e-15
Identity = 80/308 (25.97%), Postives = 139/308 (45.13%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTL--EFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILK---------LVRFMPALR---QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTCDR----------KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQH 2961
             VN   F   +L+ I +N +++ +E    P   T+ L    +N VF+++F +EM +K++  G+      Y  +G+N+ DG +V + +I+L     +   S  R F +LR+ +         ++   P L+   Q L   +R I N+     +   F  IF ILG+ LF   F  C  EN     N   C R           NFD L  +++++F + +++ W  ++Y G+               W  LYFIA +    + + N+ V ++VE F   R +EQ+     R++ K+     K+ R M  
Sbjct: 2158 FVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISE--SSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIV---LICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCR-EEQEKEEKIRRAAKRALQMEKKRRRMHE 2459          

HSP 6 Score: 81.2629 bits (199), Expect = 1.380e-14
Identity = 60/173 (34.68%), Postives = 93/173 (53.76%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  ++    F+ + +  I LNCITL M +P +     +     L + + I    F  EM IK+++ G+  G   YL   WN +D F+V   LL+  +     H   +   L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL++G
Sbjct:  291 RNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGI-CGKNTYLADSWNRLDFFIVLAGLLEYVM-----HVENLN--LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEG 455          

HSP 7 Score: 72.4034 bits (176), Expect = 5.636e-12
Identity = 52/207 (25.12%), Postives = 104/207 (50.24%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-----------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-NNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            +  +V    F   I   I +N ++M +EY++ P  L   L+  N  F+ +F++E  +KL+  G   Y+K+ +N  D  IV+L ++ +  +   +            + ++R  R+ R+LKL++    +R  L  +++ +  V     L  L  FIF+ LG+ LFG   C+ E   +   +  +F +   + +T+F++ T ++WN ++
Sbjct: 2473 VHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIM 2679          

HSP 8 Score: 67.0106 bits (162), Expect = 2.211e-10
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQ--IMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPL---------RAINRIPSMRI 756
             +IF ++   R  C   +   WF+ + +  IA+NCITL+M +P    N   S  +   +   +Y+    FT EM IK++A G F G   YF   WN +D  +V +  I++L S    I  ++ R   +LR           R INR P +++
Sbjct: 2142 LYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERP----NIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSL---ISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKL 2286          

HSP 9 Score: 67.0106 bits (162), Expect = 2.248e-10
Identity = 71/228 (31.14%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 4819 RRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFM----PINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHA-----------------HFENFGMAFLTLFRIATGDNWNGIM 5439
            RR I  +V  KYF   I   I +N ++M  E+H  P  L   ++  N  F+  F +E LLK+VA G  RYI++ +N  D +IVILS       E+   FM        + + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C  ++   GL R                   HF N   A +T+F+I T ++WN ++
Sbjct: 1221 RRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAI-----EICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDES-GLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVL 1442          

HSP 10 Score: 56.225 bits (134), Expect = 5.260e-7
Identity = 37/136 (27.21%), Postives = 75/136 (55.15%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQP-------VQLTQTLE-FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            L+ +  F+R  ++ IL+N +++G+    QP           + L+ F +I+F+  F +EM +K++  GI G   Y+ + +N  D  IV+ G++E         L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:  297 LITNPWFERVSILVILLNCITLGM---YQPCVDDACVTNRCKILQIFDDIIFA-FFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTL 428          

HSP 11 Score: 54.6842 bits (130), Expect = 1.313e-6
Identity = 27/94 (28.72%), Postives = 53/94 (56.38%), Query Frame = 1
Query: 4432 CLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            C +  +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ +FF++N+ + V+   F + ++ +
Sbjct:  540 CKQSGENPFQG-TISFDNIGMAWVAIFLVISLEGWTDIMYYVQDA----------HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKRE 622          

HSP 12 Score: 53.9138 bits (128), Expect = 2.465e-6
Identity = 26/88 (29.55%), Postives = 48/88 (54.55%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIER 1428
            NFD++G A +++F + S + WV+IMY   DA          ++ W  +YF+A I++  FF++N+ + V+   F   +  + +   I R
Sbjct: 2355 NFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRR 2442          

HSP 13 Score: 53.5286 bits (127), Expect = 3.391e-6
Identity = 21/64 (32.81%), Postives = 40/64 (62.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFS 1386
            +F+NI +A V +FQ+++ E W  +++   +  S W  +YFVAL+  G++ + NL + ++   FS
Sbjct: 1418 HFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFS 1481          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Fly
Match: Ca-alpha1T (gene:FBgn0264386 transcript:FBtr0089393)

HSP 1 Score: 784.637 bits (2025), Expect = 0.000e+0
Identity = 446/636 (70.13%), Postives = 534/636 (83.96%), Query Frame = 1
Query: 3799 PKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN---FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQL 5697
            PK    +R  +SLY+F  DN+FRQ C   V++K FD +VL FI LNCITLAMERP+IPP S ERLFL   NY+FT++FT EM IKV++ G+  G  AY  SGWNIMDG LV IS++DL ++++   S RIFGILRVFRLLR+LRPLRVI+RAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC+G +++ V+  ++C +   N W N+KYNFD+LG+ALM+LFVLSS+DGWV IMYTG+DAVGV+ QP  N+NEW L+YFI+F+LLV FFVLNMFVGVVVENFH+C+E QEKEEK RRAAKRA ++EKKR+ +  P Y+ NYS  R  +++VVTSKYFDLAIA VIGLNV+TMA E++ MP  LK ALK+FNYFFT+ F+LEA +KLVALG   Y+ D+WNQLD+ IV+LSI G+V++E+E+N    +PINPTI+RVMRVLRIARVLKLLKMA+GIRALLDTV+QALPQVGNLGLLFFLLF+IFAALGVELFGRLECSD  PC GLG HAHF NFGMAFLTLFR+ATGDNWNGIMKDTLR+ C  + DC RNCCVS++IAPI+FVIFVLMAQFVLVNVVVAVLMKHLEESHK M D+ ++E E++R+L  E +E A+E++L
Sbjct: 2113 PKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDMEVELERELVRE-QEFAQEQKL 2747          

HSP 2 Score: 417.157 bits (1071), Expect = 3.265e-117
Identity = 249/435 (57.24%), Postives = 308/435 (70.80%), Query Frame = 1
Query:  295 PNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKD--EPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQG 1593
            PN  L Y GF         Q   PR+WCL +IT PWFE++S+ +I +NCITL MYQPC D  C T++C+I+Q FD IIFAFF  EM IK++AMG  G   Y  D+WNRLD FIV+ G +E +     N+ LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W+G+LR RC L   E   +P L LS +Y+ ++       D+D+ICST  ++G+  C + PP  +     SL +C + A+ F  N PT  SCVNWNQYYT C+   +NP++G I+FDNIG AWVAIF VIS+E W DIMYYVQDAHSFWDWIYFV LI+IGSFFMINLCLVVIA+QFSETK+RE ERM  ER R+ S++  + S +  EP TCY EI+K I  +  +  RR++K Y   + +  +  +G
Sbjct:  269 PN--LPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHV-ENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYP-LTLSQYYEFSK-------DQDYICSTPNDSGMHLCGNFPPYRI----GSL-VCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEG 687          

HSP 3 Score: 322.783 bits (826), Expect = 4.264e-88
Identity = 181/272 (66.54%), Postives = 212/272 (77.94%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQ-------QGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS--------------ERLDEQKLLHNYRQSL 2916
            I+ LV    FQ+GIL+AILINTLSMGIEYH QP +LT  +E SN+VFS +F +EM+LK++ EG   YI NGFN+FDGIIV+L  IE+CQ        GG SGLS+LRTFRLLRILKLVRFMP LR+QLFVM+RT+DNVA FF+LL+LFIFIFSILGM+LFGCKFC   N +  CDRKNFDSLLW++VTVFQILTQEDWN VL+NGM +TS WAALYF+ALMTFGNYVLFNLLVAILVEGFSS              ++L E+ L  NY   +
Sbjct: 1224 IRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKFCEKVNGEMICDRKNFDSLLWALVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKKLREETLAENYSDGM 1495          

HSP 4 Score: 107.457 bits (267), Expect = 1.278e-22
Identity = 87/343 (25.36%), Postives = 159/343 (46.36%), Query Frame = 1
Query: 3706 ESQNSSRMNNVEDPDEEDMEAMIC--------LSKCCPQPKGIF-AKRYYHSLY--VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDL---FITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            + Q +SRM     P    ME   C        LS    +P+      R   S+Y  +  V +  R+  + +V+ K F   +L  I +N +++ +E  + PP       +  +N +F+ IF  EM++KV+++G       Y+ +G+N+ DG +V +S +++   F+    G       +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF  KF                C K +  E    + NFD+L  AL+ +F + +++ W  +++ G+          E  + WA +YF++ +    + + N+ V ++VE F
Sbjct: 1157 QGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTA--IVETSNVVFSGIFAVEMLLKVVAEGPF----RYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFM---PNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKF----------------CEKVN-GEMICDRKNFDSLLWALVTVFQILTQEDWNVVLFNGM----------EKTSHWAALYFVALMTFGNYVLFNLLVAILVEGF 1463          

HSP 5 Score: 82.0333 bits (201), Expect = 7.343e-15
Identity = 80/308 (25.97%), Postives = 139/308 (45.13%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTL--EFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILK---------LVRFMPALR---QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTCDR----------KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQH 2961
             VN   F   +L+ I +N +++ +E    P   T+ L    +N VF+++F +EM +K++  G+      Y  +G+N+ DG +V + +I+L     +   S  R F +LR+ +         ++   P L+   Q L   +R I N+     +   F  IF ILG+ LF   F  C  EN     N   C R           NFD L  +++++F + +++ W  ++Y G+               W  LYFIA +    + + N+ V ++VE F   R +EQ+     R++ K+     K+ R M  
Sbjct: 2141 FVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISE--SSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIV---LICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCR-EEQEKEEKIRRAAKRALQMEKKRRRMHE 2442          

HSP 6 Score: 81.2629 bits (199), Expect = 1.289e-14
Identity = 60/173 (34.68%), Postives = 93/173 (53.76%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  ++    F+ + +  I LNCITL M +P +     +     L + + I    F  EM IK+++ G+  G   YL   WN +D F+V   LL+  +     H   +   L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL++G
Sbjct:  291 RNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGI-CGKNTYLADSWNRLDFFIVLAGLLEYVM-----HVENLN--LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEG 455          

HSP 7 Score: 72.4034 bits (176), Expect = 5.633e-12
Identity = 52/207 (25.12%), Postives = 104/207 (50.24%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-----------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-NNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            +  +V    F   I   I +N ++M +EY++ P  L   L+  N  F+ +F++E  +KL+  G   Y+K+ +N  D  IV+L ++ +  +   +            + ++R  R+ R+LKL++    +R  L  +++ +  V     L  L  FIF+ LG+ LFG   C+ E   +   +  +F +   + +T+F++ T ++WN ++
Sbjct: 2456 VHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIM 2662          

HSP 8 Score: 71.2478 bits (173), Expect = 1.360e-11
Identity = 68/211 (32.23%), Postives = 110/211 (52.13%), Query Frame = 1
Query: 4819 RRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFM----PINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIM 5439
            RR I  +V  KYF   I   I +N ++M  E+H  P  L   ++  N  F+  F +E LLK+VA G  RYI++ +N  D +IVILS       E+   FM        + + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C  +N  +   R  +F++   A +T+F+I T ++WN ++
Sbjct: 1221 RRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAI-----EICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKFCEKVNGEMICDRK-NFDSLLWALVTVFQILTQEDWNVVL 1425          

HSP 9 Score: 67.3958 bits (163), Expect = 2.173e-10
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQ--IMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPL---------RAINRIPSMRI 756
             +IF ++   R  C   +   WF+ + +  IA+NCITL+M +P    N   S  +   +   +Y+    FT EM IK++A G F G   YF   WN +D  +V +  I++L S    I  ++ R   +LR           R INR P +++
Sbjct: 2125 LYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERP----NIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSL---ISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKL 2269          

HSP 10 Score: 55.8398 bits (133), Expect = 5.576e-7
Identity = 37/136 (27.21%), Postives = 75/136 (55.15%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQP-------VQLTQTLE-FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            L+ +  F+R  ++ IL+N +++G+    QP           + L+ F +I+F+  F +EM +K++  GI G   Y+ + +N  D  IV+ G++E         L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:  297 LITNPWFERVSILVILLNCITLGM---YQPCVDDACVTNRCKILQIFDDIIFA-FFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTL 428          

HSP 11 Score: 54.6842 bits (130), Expect = 1.369e-6
Identity = 21/64 (32.81%), Postives = 40/64 (62.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFS 1386
            NFD++ +A V +FQ+++ E W  +++   +  S W  +YFVAL+  G++ + NL + ++   FS
Sbjct: 1401 NFDSLLWALVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFS 1464          

HSP 12 Score: 54.6842 bits (130), Expect = 1.381e-6
Identity = 27/94 (28.72%), Postives = 53/94 (56.38%), Query Frame = 1
Query: 4432 CLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            C +  +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ +FF++N+ + V+   F + ++ +
Sbjct:  540 CKQSGENPFQG-TISFDNIGMAWVAIFLVISLEGWTDIMYYVQDA----------HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKRE 622          

HSP 13 Score: 53.9138 bits (128), Expect = 2.506e-6
Identity = 26/88 (29.55%), Postives = 48/88 (54.55%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIER 1428
            NFD++G A +++F + S + WV+IMY   DA          ++ W  +YF+A I++  FF++N+ + V+   F   +  + +   I R
Sbjct: 2338 NFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRR 2425          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Fly
Match: Ca-alpha1T (gene:FBgn0264386 transcript:FBtr0304610)

HSP 1 Score: 784.252 bits (2024), Expect = 0.000e+0
Identity = 446/636 (70.13%), Postives = 534/636 (83.96%), Query Frame = 1
Query: 3799 PKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN---FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQL 5697
            PK    +R  +SLY+F  DN+FRQ C   V++K FD +VL FI LNCITLAMERP+IPP S ERLFL   NY+FT++FT EM IKV++ G+  G  AY  SGWNIMDG LV IS++DL ++++   S RIFGILRVFRLLR+LRPLRVI+RAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC+G +++ V+  ++C +   N W N+KYNFD+LG+ALM+LFVLSS+DGWV IMYTG+DAVGV+ QP  N+NEW L+YFI+F+LLV FFVLNMFVGVVVENFH+C+E QEKEEK RRAAKRA ++EKKR+ +  P Y+ NYS  R  +++VVTSKYFDLAIA VIGLNV+TMA E++ MP  LK ALK+FNYFFT+ F+LEA +KLVALG   Y+ D+WNQLD+ IV+LSI G+V++E+E+N    +PINPTI+RVMRVLRIARVLKLLKMA+GIRALLDTV+QALPQVGNLGLLFFLLF+IFAALGVELFGRLECSD  PC GLG HAHF NFGMAFLTLFR+ATGDNWNGIMKDTLR+ C  + DC RNCCVS++IAPI+FVIFVLMAQFVLVNVVVAVLMKHLEESHK M D+ ++E E++R+L  E +E A+E++L
Sbjct: 2125 PKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDMEVELERELVRE-QEFAQEQKL 2759          

HSP 2 Score: 416.772 bits (1070), Expect = 4.757e-117
Identity = 249/426 (58.45%), Postives = 305/426 (71.60%), Query Frame = 1
Query:  295 PNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKD--EPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQ 1566
            PN  L Y GF         Q   PR+WCL +IT PWFE++S+ +I +NCITL MYQPC D  C T++C+I+Q FD IIFAFF  EM IK++AMG  G   Y  D+WNRLD FIV+ G +E +     N+ LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W+G+LR RC L   E   +P L LS +Y+ ++       D+D+ICST  ++G+  C + PP  +     SL +C + A+ F  N PT  SCVNWNQYYT C+   +NP++G I+FDNIG AWVAIF VIS+E W DIMYYVQDAHSFWDWIYFV LI+IGSFFMINLCLVVIA+QFSETK+RE ERM  ER R+ S++  + S +  EP TCY EI+K I  +  +  RR++K Y + Q
Sbjct:  269 PN--LPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHV-ENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYP-LTLSQYYEFSK-------DQDYICSTPNDSGMHLCGNFPPYRI----GSL-VCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYQRQQ 678          

HSP 3 Score: 298.13 bits (762), Expect = 1.509e-80
Identity = 175/289 (60.55%), Postives = 209/289 (72.32%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQ-------QGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTI-----------------ENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS--------------ERLDEQKLLHNYRQSL 2916
            I+ LV    FQ+GIL+AILINTLSMGIEYH QP +LT  +E SN+VFS +F +EM+LK++ EG   YI NGFN+FDGIIV+L  IE+CQ        GG SGLS+LRTFRLLRILKLVRFMP LR+QLFVM+RT+DNVA FF+LL+LFIFIFSILGM LFG KFC                    + +  CDRK+F+++LW+ VTVFQILTQEDWN VL+NGM +TS WAALYF+ALMTFGNYVLFNLLVAILVEGFSS              ++L E+ L  NY   +
Sbjct: 1219 IRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKKLREETLAENYSDGM 1507          

HSP 4 Score: 107.842 bits (268), Expect = 1.216e-22
Identity = 87/345 (25.22%), Postives = 166/345 (48.12%), Query Frame = 1
Query: 3706 ESQNSSRMNNVEDPDEEDMEAMIC--------LSKCCPQPKGIF-AKRYYHSLY--VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDL---FITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF-YYCDGPHLQAVKTKNDCL-KYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            + Q +SRM     P    ME   C        LS    +P+      R   S+Y  +  V +  R+  + +V+ K F   +L  I +N +++ +E  + PP       +  +N +F+ IF  EM++KV+++G       Y+ +G+N+ DG +V +S +++   F+    G       +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF GKF  + D   L+   T  + + ++ Q E    + +F+N+  A + +F + +++ W  +++ G+          E  + WA +YF++ +    + + N+ V ++VE F
Sbjct: 1152 QGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTA--IVETSNVVFSGIFAVEMLLKVVAEGPF----RYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFM---PNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCEC--DRKHFNNILWATVTVFQILTQEDWNVVLFNGM----------EKTSHWAALYFVALMTFGNYVLFNLLVAILVEGF 1475          

HSP 5 Score: 82.0333 bits (201), Expect = 7.224e-15
Identity = 80/308 (25.97%), Postives = 139/308 (45.13%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTL--EFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILK---------LVRFMPALR---QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTCDR----------KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQH 2961
             VN   F   +L+ I +N +++ +E    P   T+ L    +N VF+++F +EM +K++  G+      Y  +G+N+ DG +V + +I+L     +   S  R F +LR+ +         ++   P L+   Q L   +R I N+     +   F  IF ILG+ LF   F  C  EN     N   C R           NFD L  +++++F + +++ W  ++Y G+               W  LYFIA +    + + N+ V ++VE F   R +EQ+     R++ K+     K+ R M  
Sbjct: 2153 FVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISE--SSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIV---LICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCR-EEQEKEEKIRRAAKRALQMEKKRRRMHE 2454          

HSP 6 Score: 80.8777 bits (198), Expect = 1.438e-14
Identity = 60/173 (34.68%), Postives = 93/173 (53.76%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  ++    F+ + +  I LNCITL M +P +     +     L + + I    F  EM IK+++ G+  G   YL   WN +D F+V   LL+  +     H   +   L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL++G
Sbjct:  291 RNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGI-CGKNTYLADSWNRLDFFIVLAGLLEYVM-----HVENLN--LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEG 455          

HSP 7 Score: 72.4034 bits (176), Expect = 5.877e-12
Identity = 52/207 (25.12%), Postives = 104/207 (50.24%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-----------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-NNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            +  +V    F   I   I +N ++M +EY++ P  L   L+  N  F+ +F++E  +KL+  G   Y+K+ +N  D  IV+L ++ +  +   +            + ++R  R+ R+LKL++    +R  L  +++ +  V     L  L  FIF+ LG+ LFG   C+ E   +   +  +F +   + +T+F++ T ++WN ++
Sbjct: 2468 VHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIM 2674          

HSP 8 Score: 67.0106 bits (162), Expect = 2.286e-10
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQ--IMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPL---------RAINRIPSMRI 756
             +IF ++   R  C   +   WF+ + +  IA+NCITL+M +P    N   S  +   +   +Y+    FT EM IK++A G F G   YF   WN +D  +V +  I++L S    I  ++ R   +LR           R INR P +++
Sbjct: 2137 LYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERP----NIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSL---ISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKL 2281          

HSP 9 Score: 67.0106 bits (162), Expect = 2.615e-10
Identity = 71/228 (31.14%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 4819 RRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFM----PINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHA-----------------HFENFGMAFLTLFRIATGDNWNGIM 5439
            RR I  +V  KYF   I   I +N ++M  E+H  P  L   ++  N  F+  F +E LLK+VA G  RYI++ +N  D +IVILS       E+   FM        + + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C  ++   GL R                   HF N   A +T+F+I T ++WN ++
Sbjct: 1216 RRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAI-----EICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDES-GLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVL 1437          

HSP 10 Score: 55.8398 bits (133), Expect = 5.485e-7
Identity = 37/136 (27.21%), Postives = 75/136 (55.15%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQP-------VQLTQTLE-FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            L+ +  F+R  ++ IL+N +++G+    QP           + L+ F +I+F+  F +EM +K++  GI G   Y+ + +N  D  IV+ G++E         L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:  297 LITNPWFERVSILVILLNCITLGM---YQPCVDDACVTNRCKILQIFDDIIFA-FFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTL 428          

HSP 11 Score: 54.6842 bits (130), Expect = 1.324e-6
Identity = 27/94 (28.72%), Postives = 53/94 (56.38%), Query Frame = 1
Query: 4432 CLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            C +  +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ +FF++N+ + V+   F + ++ +
Sbjct:  540 CKQSGENPFQG-TISFDNIGMAWVAIFLVISLEGWTDIMYYVQDA----------HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKRE 622          

HSP 12 Score: 53.9138 bits (128), Expect = 2.465e-6
Identity = 26/88 (29.55%), Postives = 48/88 (54.55%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIER 1428
            NFD++G A +++F + S + WV+IMY   DA          ++ W  +YF+A I++  FF++N+ + V+   F   +  + +   I R
Sbjct: 2350 NFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRR 2437          

HSP 13 Score: 53.5286 bits (127), Expect = 3.391e-6
Identity = 21/64 (32.81%), Postives = 40/64 (62.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFS 1386
            +F+NI +A V +FQ+++ E W  +++   +  S W  +YFVAL+  G++ + NL + ++   FS
Sbjct: 1413 HFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFS 1476          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Fly
Match: Ca-alpha1T (gene:FBgn0264386 transcript:FBtr0334984)

HSP 1 Score: 784.252 bits (2024), Expect = 0.000e+0
Identity = 446/636 (70.13%), Postives = 534/636 (83.96%), Query Frame = 1
Query: 3799 PKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN---FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQL 5697
            PK    +R  +SLY+F  DN+FRQ C   V++K FD +VL FI LNCITLAMERP+IPP S ERLFL   NY+FT++FT EM IKV++ G+  G  AY  SGWNIMDG LV IS++DL ++++   S RIFGILRVFRLLR+LRPLRVI+RAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC+G +++ V+  ++C +   N W N+KYNFD+LG+ALM+LFVLSS+DGWV IMYTG+DAVGV+ QP  N+NEW L+YFI+F+LLV FFVLNMFVGVVVENFH+C+E QEKEEK RRAAKRA ++EKKR+ +  P Y+ NYS  R  +++VVTSKYFDLAIA VIGLNV+TMA E++ MP  LK ALK+FNYFFT+ F+LEA +KLVALG   Y+ D+WNQLD+ IV+LSI G+V++E+E+N    +PINPTI+RVMRVLRIARVLKLLKMA+GIRALLDTV+QALPQVGNLGLLFFLLF+IFAALGVELFGRLECSD  PC GLG HAHF NFGMAFLTLFR+ATGDNWNGIMKDTLR+ C  + DC RNCCVS++IAPI+FVIFVLMAQFVLVNVVVAVLMKHLEESHK M D+ ++E E++R+L  E +E A+E++L
Sbjct: 2143 PKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHKQMEDELDMEVELERELVRE-QEFAQEQKL 2777          

HSP 2 Score: 409.838 bits (1052), Expect = 7.466e-115
Identity = 247/441 (56.01%), Postives = 308/441 (69.84%), Query Frame = 1
Query:  295 PNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTL------FLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKD--EPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQG 1593
            PN  L Y GF         Q   PR+WCL +IT PWFE++S+ +I +NCITL MYQPC D  C T++C+I+Q FD IIFAFF  EM IK++AMG  G   Y  D+WNRLD FIV+ G +E +     N+ LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W+G+LR RC L   E   +P L +   Y++   +      +  + D+D+ICST  ++G+  C + PP  +     SL +C + A+ F  N PT  SCVNWNQYYT C+   +NP++G I+FDNIG AWVAIF VIS+E W DIMYYVQDAHSFWDWIYFV LI+IGSFFMINLCLVVIA+QFSETK+RE ERM  ER R+ S++  + S +  EP TCY EI+K I  +  +  RR++K Y   + +  +  +G
Sbjct:  269 PN--LPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHV-ENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYP-LTVPEVYRVRVRVNSLSQYYEFSKDQDYICSTPNDSGMHLCGNFPPYRI----GSL-VCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEG 700          

HSP 3 Score: 298.13 bits (762), Expect = 1.453e-80
Identity = 175/289 (60.55%), Postives = 209/289 (72.32%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQ-------QGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTI-----------------ENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS--------------ERLDEQKLLHNYRQSL 2916
            I+ LV    FQ+GIL+AILINTLSMGIEYH QP +LT  +E SN+VFS +F +EM+LK++ EG   YI NGFN+FDGIIV+L  IE+CQ        GG SGLS+LRTFRLLRILKLVRFMP LR+QLFVM+RT+DNVA FF+LL+LFIFIFSILGM LFG KFC                    + +  CDRK+F+++LW+ VTVFQILTQEDWN VL+NGM +TS WAALYF+ALMTFGNYVLFNLLVAILVEGFSS              ++L E+ L  NY   +
Sbjct: 1237 IRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKKLREETLAENYSDGM 1525          

HSP 4 Score: 107.457 bits (267), Expect = 1.258e-22
Identity = 87/345 (25.22%), Postives = 166/345 (48.12%), Query Frame = 1
Query: 3706 ESQNSSRMNNVEDPDEEDMEAMIC--------LSKCCPQPKGIF-AKRYYHSLY--VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDL---FITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF-YYCDGPHLQAVKTKNDCL-KYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            + Q +SRM     P    ME   C        LS    +P+      R   S+Y  +  V +  R+  + +V+ K F   +L  I +N +++ +E  + PP       +  +N +F+ IF  EM++KV+++G       Y+ +G+N+ DG +V +S +++   F+    G       +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF GKF  + D   L+   T  + + ++ Q E    + +F+N+  A + +F + +++ W  +++ G+          E  + WA +YF++ +    + + N+ V ++VE F
Sbjct: 1170 QGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTA--IVETSNVVFSGIFAVEMLLKVVAEGPF----RYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFM---PNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCEC--DRKHFNNILWATVTVFQILTQEDWNVVLFNGM----------EKTSHWAALYFVALMTFGNYVLFNLLVAILVEGF 1493          

HSP 5 Score: 82.0333 bits (201), Expect = 6.991e-15
Identity = 80/308 (25.97%), Postives = 139/308 (45.13%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTL--EFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILK---------LVRFMPALR---QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTCDR----------KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQH 2961
             VN   F   +L+ I +N +++ +E    P   T+ L    +N VF+++F +EM +K++  G+      Y  +G+N+ DG +V + +I+L     +   S  R F +LR+ +         ++   P L+   Q L   +R I N+     +   F  IF ILG+ LF   F  C  EN     N   C R           NFD L  +++++F + +++ W  ++Y G+               W  LYFIA +    + + N+ V ++VE F   R +EQ+     R++ K+     K+ R M  
Sbjct: 2171 FVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISE--SSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIV---LICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCR-EEQEKEEKIRRAAKRALQMEKKRRRMHE 2472          

HSP 6 Score: 80.8777 bits (198), Expect = 1.427e-14
Identity = 60/173 (34.68%), Postives = 93/173 (53.76%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  ++    F+ + +  I LNCITL M +P +     +     L + + I    F  EM IK+++ G+  G   YL   WN +D F+V   LL+  +     H   +   L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL++G
Sbjct:  291 RNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGI-CGKNTYLADSWNRLDFFIVLAGLLEYVM-----HVENLN--LTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEG 455          

HSP 7 Score: 72.4034 bits (176), Expect = 5.686e-12
Identity = 52/207 (25.12%), Postives = 104/207 (50.24%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-----------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIE-NNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            +  +V    F   I   I +N ++M +EY++ P  L   L+  N  F+ +F++E  +KL+  G   Y+K+ +N  D  IV+L ++ +  +   +            + ++R  R+ R+LKL++    +R  L  +++ +  V     L  L  FIF+ LG+ LFG   C+ E   +   +  +F +   + +T+F++ T ++WN ++
Sbjct: 2486 VHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIM 2692          

HSP 8 Score: 67.3958 bits (163), Expect = 2.157e-10
Identity = 46/152 (30.26%), Postives = 73/152 (48.03%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQ--IMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPL---------RAINRIPSMRI 756
             +IF ++   R  C   +   WF+ + +  IA+NCITL+M +P    N   S  +   +   +Y+    FT EM IK++A G F G   YF   WN +D  +V +  I++L S    I  ++ R   +LR           R INR P +++
Sbjct: 2155 LYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERP----NIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSL---ISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKL 2299          

HSP 9 Score: 67.0106 bits (162), Expect = 2.326e-10
Identity = 71/228 (31.14%), Postives = 110/228 (48.25%), Query Frame = 1
Query: 4819 RRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFM----PINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHA-----------------HFENFGMAFLTLFRIATGDNWNGIM 5439
            RR I  +V  KYF   I   I +N ++M  E+H  P  L   ++  N  F+  F +E LLK+VA G  RYI++ +N  D +IVILS       E+   FM        + + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C  ++   GL R                   HF N   A +T+F+I T ++WN ++
Sbjct: 1234 RRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAI-----EICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDES-GLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVL 1455          

HSP 10 Score: 56.225 bits (134), Expect = 5.306e-7
Identity = 37/136 (27.21%), Postives = 75/136 (55.15%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQP-------VQLTQTLE-FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            L+ +  F+R  ++ IL+N +++G+    QP           + L+ F +I+F+  F +EM +K++  GI G   Y+ + +N  D  IV+ G++E         L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:  297 LITNPWFERVSILVILLNCITLGM---YQPCVDDACVTNRCKILQIFDDIIFA-FFALEMTIKMVAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTL 428          

HSP 11 Score: 54.6842 bits (130), Expect = 1.325e-6
Identity = 27/94 (28.72%), Postives = 53/94 (56.38%), Query Frame = 1
Query: 4432 CLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            C +  +N ++    +FDN+G A +A+F++ S +GW  IMY   DA          H+ W  IYF+  +++ +FF++N+ + V+   F + ++ +
Sbjct:  553 CKQSGENPFQG-TISFDNIGMAWVAIFLVISLEGWTDIMYYVQDA----------HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKRE 635          

HSP 12 Score: 53.9138 bits (128), Expect = 2.507e-6
Identity = 26/88 (29.55%), Postives = 48/88 (54.55%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIER 1428
            NFD++G A +++F + S + WV+IMY   DA          ++ W  +YF+A I++  FF++N+ + V+   F   +  + +   I R
Sbjct: 2368 NFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRR 2455          

HSP 13 Score: 53.5286 bits (127), Expect = 3.364e-6
Identity = 21/64 (32.81%), Postives = 40/64 (62.50%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFS 1386
            +F+NI +A V +FQ+++ E W  +++   +  S W  +YFVAL+  G++ + NL + ++   FS
Sbjct: 1431 HFNNILWATVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFS 1494          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ha (calcium channel, voltage-dependent, T type, alpha 1H subunit a [Source:ZFIN;Acc:ZDB-GENE-130103-1])

HSP 1 Score: 1286.94 bits (3329), Expect = 0.000e+0
Identity = 874/1848 (47.29%), Postives = 1160/1848 (62.77%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPP---SIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKT------------------AQGNYQNKL-----KRQRKHCNVCLNSRSQFPI-----DDHCHLYSI--------------VNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVL-------NSPRHPNHLISDISSKSVSTETKRKSQYN---------------------------------SQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVXXXXXXXXXXXXXXXQSILSINKSFETTSSRNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXH-LRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELA 5682
            M +I +NC+TL MYQPC D  C++  C ++QAFD  IFAFF  EMVIK+IA+G  G  GY GDTWNRLD FIV+ G +E     G+N  L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC++      ++  L+  S Y  N     +  D  FICS+ K+NG+ +CS +PP    +    N S           T N    +SCVNWNQYY++C+PG+ NP++GA+NFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QF+ETK+RE   M  +R R+ S++ +  S  EP +CY E++K ++ +  K+ RR+ ++YN  Q +  K                     G++   +     + QR+HC+  L++ S  P+      +   + SI               + +S   Q  ++I  Y  +F   P + +P    +  L       N P     LI + S      + + KS  N                                 +Q+LLE+ S P         Y      +  L   D  YP+   S    +   +Y    +T   + H+    + QN+  +  +    ++++  +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F++EM+ KL+  GI GYI+N +N+FDG+IVV+ V E+       GLS+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIF FSILGM LFGCKF   +EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS WAALYF+ALMTFGNYVLFNLLVAILVEGF +E  D  K         +++ + N + ++ Q QS      + +   D  G     +  PP+I+ TAATPMPTP+SS                        L     F+ T SR  +   +  +A     + + +++           P    P  +G N         + + RS++    + L R  S+       + +R  +L  E +   E D+    + ++           + L IP   +    LD  G+S       ++LA   A    +DS +               EED++   C  L +     K  + K +   SLY+FS  N+FR  CQ ++  K FDY+VL FI LNCIT+A+ERP I     ERLFL V+NY+FT+IF  EM +KV++ G   G+Q+YLKS WN++DG LVF+SL+D+ ++ L    NRIFGILRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICC FFI+FGILGVQLFKGKF++C+G   + +  K+DCL+ +  +W  +KYNFDNLGQALM+LFVLS KDGWV IMY G+DAVGV+ QP+ NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+ E      KR + +EKKR+  LR P Y+ +YS  R  I+++ T+ Y DL I G+I +NV+TM+ E    P  L  ALK  NY FT  F++EALLKLVA G+ R+  D+WNQLD+ IV+LSI G+ ++E++ N  +PINPTI+R+MRVLRIARVLKLLKMA+G+R+LLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+LEC++ NPC GL  HA FENFGMAFLTLFR++TGDNWNGIMKDTLRE C+ SE      C+S +  ++PIYFV FVLMAQFVLVNVVVAVLMKHLEES+K   +D E++  ++  + +E + L+
Sbjct:    1 MLVILLNCVTLGMYQPCEDLKCQSEWCIVLQAFDDCIFAFFAVEMVIKMIALGIFGINGYLGDTWNRLDFFIVMAGMME-YSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMPSKVRDQY-NLSYMSLYYENE----DGEDNPFICSSSKDNGMKRCSAVPPLKEGVECTLNASFLGHVYSGLSGTGN----YSCVNWNQYYSECKPGDLNPHKGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFAETKQRENMLMKEQRARYRSNDSTLASYSEPGSCYEEMLKYVSHLYRKVKRRLSRIYNSWQSKRRKKVNPNTGAGGGANGHSRHRGYGHWVQSIHSLIQQHQRQHCH--LSNGSPSPVATTGSSEALEMRSITAGQQLTVSSQPTATHNLSSSTQSVHSI--YQGDFREAPQERTPTNVAVPALSRLNGGMNYPTILPSLICNYSGGGSLGKERGKSDANTGHEVIQDFDKLHQQIEAHSHSRVLLQMAGVVPTQLLLEVMSCP---------YCVRALESMDLEIGDSDYPR---SEGDMVYEFSYDAGFITGRSAHHK--MQEKQNRLVQ--FWEDFRERLTRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNILEISNIVFTSMFVLEMLFKLLAFGIFGYIRNPYNIFDGVIVVISVWEIIGH-ADGGLSVLRTFRLLRVLKLVRFLPALRRQLLVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSLKMENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEG-DANK-----SDGDEEKTSVNSEEKMEQLQS------SANGHVDPCG-----TLPPPIIMRTAATPMPTPKSS------------------------LGPESVFDLTESRRGSAVSIDPNAFDQKSLSSPRRS-----------PCH--PRGSGSN---------WGSRRSSW----NSLGRAPSLKR--KDTSGERESLLSGEGRGSFEDDDIEAEKLTNISG--GSLQHPSSLDIPELPQMGEYLDCNGRS------QHILADLSASLNKEDSIA---------------EEDLDDSFCFRLRRTLAAYKPKWCKDHEEWSLYLFSPHNKFRMMCQKLISHKMFDYVVLVFIFLNCITIALERPHI--QQSERLFLLVSNYVFTVIFVAEMTVKVVALGFYSGNQSYLKSTWNVLDGVLVFVSLIDILVS-LAWTGNRIFGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFFHCEGGDTRNITNKSDCLQ-ANLKWIRRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPERNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEVEARLLELKRQKLMEKKRRKALRRP-YYADYSPARLYIHTLCTNHYLDLFITGIICINVVTMSIEHFNQPSYLDEALKYCNYVFTIIFIIEALLKLVAFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRSLLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECTENNPCEGLSPHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE-CLPSE----TQCLSYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAAIKLAILKETRRLS 1715          

HSP 2 Score: 63.5438 bits (153), Expect = 3.550e-9
Identity = 38/119 (31.93%), Postives = 64/119 (53.78%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPS---TGNNI 681
             ++F  +   R  C K+I+   F+ + +  I +NCIT+++ +P    + + S+   +   +Y+    F  EM +K++A+GF  G+  Y   TWN LD  +V V  I+IL S   TGN I
Sbjct: 1106 LYLFSPHNKFRMMCQKLISHKMFDYVVLVFIFLNCITIALERP----HIQQSERLFLLVSNYVFTVIFVAEMTVKVVALGFYSGNQSYLKSTWNVLDGVLVFVSLIDILVSLAWTGNRI 1220          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1hb (calcium channel, voltage-dependent, T type, alpha 1H subunit b [Source:ZFIN;Acc:ZDB-GENE-090312-128])

HSP 1 Score: 633.639 bits (1633), Expect = 0.000e+0
Identity = 384/618 (62.14%), Postives = 490/618 (79.29%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCC--VSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKE 5676
            SLY+F+  N FR +CQ ++  K FD++VLFFI  NCIT+A+ERP I P S ER+FL V+NYIFT+IF  EM +KV+S GL  G  +YLKS WN++DGFLVF+S++D+ +++     NRI GILRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICC FFI+FGILGVQLFKGKFY+C+G   + +  K++CL+ ++ +W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAV V+ QP  NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+EE  RR  KR +R+EKKR+      Y+ +YS  R  I+++ TS Y DL I  +I +NV+TM+ E +  P+ L   LK  NYFFT  FV+EA LKLVA G  R+  ++WNQLD+ IV+LSI G+ ++E++ N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L+C++ NPC GL RHA F+NFGMAFLTLFR++TGDNWNGIMKDTLRE       C+ + C     +++P+YFV FVLMAQFVLVNVVVAVLMKHLEES+    ++ E++ E++ +L+   ++
Sbjct:  634 SLYLFAPQNNFRMRCQKVITHKMFDHIVLFFIFFNCITIALERPDIQPDSTERIFLKVSNYIFTVIFVAEMTVKVVSLGLYAGPGSYLKSSWNVLDGFLVFVSIVDILVSLASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFYHCEGFDTRNITNKSECLQ-AKYKWIRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVAVDQQPIRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARRREEKRQKRMEKKRRKALQRPYYADYSPTRLYIHTLCTSHYLDLFITIIIAINVLTMSMEHYRQPKYLDEGLKYCNYFFTLVFVIEAALKLVAFGFRRFFKERWNQLDLAIVLLSIMGITLEEIDLNASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCTEENPCEGLSRHATFQNFGMAFLTLFRVSTGDNWNGIMKDTLRE-------CKNDKCLSYLPLLSPLYFVTFVLMAQFVLVNVVVAVLMKHLEESNIDAREEAEMDAELKLELEAGRRQ 1243          

HSP 2 Score: 306.605 bits (784), Expect = 1.734e-83
Identity = 172/285 (60.35%), Postives = 217/285 (76.14%), Query Frame = 1
Query: 2077 DSYSKHRRP-FNKNQNKTKRKNYLSIC----QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER--LDEQKLLHNYR 2907
            D  S+ R+   +  +  ++++N + +C    +  +K +V+   F RGI+IAILINTLSMG+EYH+QP +LT  LE SNIVF+ LF++EM+LKL   G+  YI N +N+FDGIIV++ V E+  Q    G S+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF   +EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS +AALYF+ALMTFGNYVLFNLLVAILVEGF +E   L  ++LL  Y+
Sbjct:  340 DVGSEQRKTNLDAEEKVSEKRNCVCMCVHYARAVLKRIVDSKYFNRGIMIAILINTLSMGVEYHEQPDELTDVLEISNIVFTSLFVLEMLLKLSACGLFDYISNPYNIFDGIIVIISVCEIVGQA-DGGFSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTLRLENGDTVSDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPYAALYFVALMTFGNYVLFNLLVAILVEGFQAESWCLILKRLLEPYK 623          

HSP 3 Score: 227.254 bits (578), Expect = 4.567e-59
Identity = 113/245 (46.12%), Postives = 162/245 (66.12%), Query Frame = 1
Query:  850 QMWQGVLRNRCYLNLN-ESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK 1581
            Q+W G+LRNRC+ + N     +   ++S +Y+++ T      D  FICST++ NG+L+CS++P   +  ++  L         F +     H CVNWN YY  C   E NP+ GAINFDNIG+AW+AIFQVI++E W DIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R RF S+  +  S  EP +CY E++K +  +  K++R +V++Y  +  +  K
Sbjct:   36 QLWAGLLRNRCFFDNNIRMLYNVSGSVSEYYRVDET-----DDIPFICSTNRENGMLRCSNVPKRRVAGADCELTADNVTELVFKE--APHHGCVNWNLYYNTCNASEHNPHNGAINFDNIGYAWIAIFQVITLEGWADIMYYVMDAHSFYNFIYFIFLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARFRSNESTLASLAEPGSCYEEMLKYLAHVCRKLWRHVVRIYGDLCPKRRK 273          

HSP 4 Score: 142.124 bits (357), Expect = 4.140e-33
Identity = 150/551 (27.22%), Postives = 267/551 (48.46%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLY-WENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEF-HMMPQ-VLKTALKVFNYFFTSTFVXXXXXXXXX----XXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELF-GRL-ECSDIN--------PCVG-----LGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            R   + IVD K F+  ++  I +N +++ +E    P    +   L ++N +FT +F  EM++K+ + GL      Y+ + +NI DG +V IS+ +     ++G ++  F +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 L+    +  + + NFD+L  A++ +F + +++ W  ++Y G+ +           + +A +YF++ +    + + N+ V ++VE F       E      +      + +  R H    LY +   + +R     V+T K FD  +   I  N IT+A E   + P    +  LKV NY FT  FV E  +K+V+     G   Y+   WN LD  +V +SI  +++    S    I   I+RV+R+LR  R L+++  A G++ +++T+I +L  +GN+ L+    F +F  LGV+LF G+   C   +         C+      + R  +F+N G A ++LF +++ D W  IM D L
Sbjct:  371 RAVLKRIVDSKYFNRGIMIAILINTLSMGVEYHEQPDELTD--VLEISNIVFTSLFVLEMLLKLSACGLF----DYISNPYNIFDGIIVIISVCE-----IVGQADGGFSVLRTFRLLRVLKLVRFL---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------TLRLENGDTVSDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SPYAALYFVALMTFGNYVLFNLLVAILVEGFQA-----ESWCLILKRLLEPYKPQWCRDHEDWSLYLFAPQNNFRMRCQKVITHKMFDHIVLFFIFFNCITIALERPDIQPDSTERIFLKVSNYIFTVIFVAEMTVKVVSLGLYAGPGSYLKSSWNVLDGFLVFVSIVDILVSLASSGGNRIL-GILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFYHCEGFDTRNITNKSECLQAKYKWIRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGL 875          

HSP 5 Score: 86.2705 bits (212), Expect = 4.361e-16
Identity = 60/205 (29.27%), Postives = 106/205 (51.71%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVL 2748
            I TL         I I I IN L+M +E+++QP  L + L++ N  F+++F+IE  LKL+  G   + K  +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   CT EN      R   F +   + +T+F++ T ++WN ++
Sbjct:  965 IHTLCTSHYLDLFITIIIAINVLTMSMEHYRQPKYLDEGLKYCNYFFTLVFVIEAALKLVAFGFRRFFKERWNQLDLAIVLLSIMGITLEEIDLNASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCTEENPCEGLSRHATFQNFGMAFLTLFRVSTGDNWNGIM 1169          

HSP 6 Score: 72.4034 bits (176), Expect = 6.830e-12
Identity = 67/282 (23.76%), Postives = 137/282 (48.58%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGILG----YIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTC---------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDE 2883
            + + + ++    F   +L  I  N +++ +E    QP    +  L+ SN +F+++F+ EM +K++  G+      Y+K+ +N+ DG +V + ++++     +SG       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  +F ILG+ LF  KF   E        NK  C          + NFD+L  +++++F + +++ W  ++Y+G+   +           W  LYFI+ +   ++ + N+ V ++VE F   R D+
Sbjct:  645 RMRCQKVITHKMFDHIVLFFIFFNCITIALERPDIQPDSTERIFLKVSNYIFTVIFVAEMTVKVVSLGLYAGPGSYLKSSWNVLDGFLVFVSIVDILVSLASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFYHCEGFDTRNITNKSECLQAKYKWIRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVAVDQQPIRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQ 926          

HSP 7 Score: 63.5438 bits (153), Expect = 3.739e-9
Identity = 34/117 (29.06%), Postives = 64/117 (54.70%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F    + R  C K+IT   F+ + +  I  NCIT+++ +P  D   ++++   ++  +YI    F  EM +K++++G + G   Y   +WN LD F+V V  ++IL S  ++ G
Sbjct:  635 LYLFAPQNNFRMRCQKVITHKMFDHIVLFFIFFNCITIALERP--DIQPDSTERIFLKVSNYIFTVIFVAEMTVKVVSLGLYAGPGSYLKSSWNVLDGFLVFVSIVDILVSLASSGG 749          

HSP 8 Score: 54.6842 bits (130), Expect = 1.588e-6
Identity = 31/92 (33.70%), Postives = 52/92 (56.52%), Query Frame = 1
Query: 4444 SQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            S++   N   NFDN+G A +A+F + + +GW  IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  135 SEHNPHNGAINFDNIGYAWIAIFQVITLEGWADIMYYVMDA----------HSFYNFIYFIFLIIVGSFFMINLCLVVIATQFSETKQRENQ 216          

HSP 9 Score: 53.9138 bits (128), Expect = 2.721e-6
Identity = 23/82 (28.05%), Postives = 46/82 (56.10%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLK 2919
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R   +
Sbjct:  145 NFDNIGYAWIAIFQVITLEGWADIMYYVMDAHSFYNFIYFIFLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARFR 226          

HSP 10 Score: 53.9138 bits (128), Expect = 2.985e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct:  847 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVAVDQQPIRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQ 926          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ia (calcium voltage-gated channel subunit alpha1 Ia [Source:ZFIN;Acc:ZDB-GENE-060503-324])

HSP 1 Score: 631.713 bits (1628), Expect = 0.000e+0
Identity = 387/612 (63.24%), Postives = 486/612 (79.41%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            S+++FS  N+FR  CQ I+  K FDY+VL FI  NCIT+A+ERP I   S ERLFLTV+NYIFT IF GEM +KV+S GL IG+QAYL+S WNI+DGFLVF+SL+D+ +++  G   +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SL+PIGNIVLICC FFIIFGILGVQLFKGKFYYC G  ++ +  K+DCL  +  +W + KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAV V+ QP  N+N W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RR+EKKR+  +   Y+ +YS  R  I+++ TS Y D+ I  +I +NV+TM+ E +  P  L+ ALK  NYFFTSTFVLEA+LKL+A G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG L C++  PC G+ RHA FENFGMAFLTLF+++TGDNWNGIMKDTLRE       C  +      I+P+YFV FVL AQFVL+NVVVAVLMKHL++S+K   ++ E++ E++ +L +
Sbjct: 1043 SVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGG--AKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCL-LANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKKRRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSL---QFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMELAQ 1648          

HSP 2 Score: 332.798 bits (852), Expect = 3.072e-91
Identity = 216/395 (54.68%), Postives = 276/395 (69.87%), Query Frame = 1
Query:  400 WFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSS-FYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPP------SIMLLSNNSL--QICKQPARPFTDNLPTKHS-CVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLY 1554
            WFE++S+ +I +NC+TL MYQPC + +C + +CQ++QAFD  I+ FF  EMV+K++A+G  G   Y GDTWNRLD FIV+ G +E       NI  +AIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRCY   N +    GL L + +YQ          ++ FICS  ++NGI+ CSD+P          L   ++L  Q     A P  +   +    CVNWNQYYT C  G  NP++GAINFDNIG+AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LIIIGSFFMINLCLVVIA+QFSETK+RE + M  +R R+ SS+ +  S  EP  CY E+ +L+  I+ K  RR   LY
Sbjct:   12 WFERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDL-QNINFSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTS-GLTLPTPYYQPEED-----DERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEPLVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSS-TLASLAEPGDCYEELFQLVCHILRKARRRSAALY 398          

HSP 3 Score: 290.426 bits (742), Expect = 2.732e-78
Identity = 163/267 (61.05%), Postives = 198/267 (74.16%), Query Frame = 1
Query: 2095 RRPFNKNQNKTKRKNYLSICQQ-------KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE 2871
            +R  + +    K++  L  C+        K+  +V    F RGI+IAILINT+SMGIE+H QP +LT  LE  NIVF+ +F +EMILKL   G   Y++N +N+FDGIIV++ V E+  Q    GLS+LRTFRLLR++KLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM +FGCKF    E      DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS  AALYF+ALMTFGNYVLFNLLVAILVEGF +E
Sbjct:  511 KRSSDSDDTLKKKRTCLGKCKDVWDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCEIIGQS-DGGLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAE 776          

HSP 4 Score: 108.997 bits (271), Expect = 6.341e-23
Identity = 72/280 (25.71%), Postives = 137/280 (48.93%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            ++ R K   IV+ K F+  ++  I +N I++ +E  + P        L + N +FT +FT EMI+K+ + G       YL++ +NI DG +V IS+ +     ++G S+    +LR FRLLR ++ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ +F  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           +  A +YF++ +    + + N+ V ++VE F 
Sbjct:  535 DEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTN--VLEICNIVFTSMFTLEMILKLTAFGFF----EYLRNPYNIFDGIIVIISVCE-----IIGQSDGGLSVLRTFRLLRVIKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKF----------------SLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SPLAALYFVALMTFGNYVLFNLLVAILVEGFQ 774          

HSP 5 Score: 88.5817 bits (218), Expect = 8.586e-17
Identity = 67/266 (25.19%), Postives = 127/266 (47.74%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSW-----------AALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQK 2889
            I TL         I   I +N ++M +E++ QP  L   L++ N  F+  F++E +LKLI  G   + K+ +N  D  IV+L V+ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C   N    C+       F++   + +T+FQ+ T ++WN ++ + +                 + LYF++ +    +VL N++VA+L++       + Q+
Sbjct: 1372 IHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVC---NEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQE 1634          

HSP 6 Score: 76.2554 bits (186), Expect = 5.683e-13
Identity = 55/161 (34.16%), Postives = 92/161 (57.14%), Query Frame = 1
Query: 3901 FDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            F+ + +  I LNC+TL M +P  +I   S+    L   +    I F  EM++K+++ G+  G + YL   WN +D F+V   +++  + +     N  F  +R    +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   13 FERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIF-GRRCYLGDTWNRLDFFIVMAGMVEYSLDL----QNINFSAIRT---VRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 165          

HSP 7 Score: 70.4774 bits (171), Expect = 2.934e-11
Identity = 65/220 (29.55%), Postives = 113/220 (51.36%), Query Frame = 1
Query: 4807 YSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            + + R  +  +V SKYF+  I   I +N I+M  E H  P  L   L++ N  FTS F LE +LKL A G   Y+ + +N  D +IVI+S+   +I + +          + V+R  R+ RV+KL++    +R  L  +++ +  V    +L  L  +IF+ LG+ +FG     + E  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  534 WDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCE-IIGQSDGG--------LSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNMVLYNGM 739          

HSP 8 Score: 67.3958 bits (163), Expect = 2.152e-10
Identity = 69/272 (25.37%), Postives = 132/272 (48.53%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLE-----FSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELC--QQGGTS---GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM----------TRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQ 2886
            F   +L  I  N +++ +E   +P  L  +LE      SN +F+ +F+ EM LK++  G+      Y+++ +N+ DG +V + +I++     GG      L +LR  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C + N K    + NFD+L  +++++F + +++ W  ++Y+G+          T  + W  LYFI+ +   ++ + N+ V ++VE F   R  ++
Sbjct: 1066 FDYVVLAFIFSNCITVALE---RPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCLLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQE 1334          

HSP 9 Score: 57.3806 bits (137), Expect = 2.329e-7
Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query:  331 TVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNN 678
            +VF    QNK  R  C  II    F+ + +  I  NCIT+++ +P   Q   + +   +   +YI  A F  EM +K+++MG ++G+  Y   +WN LD F+V V  I+I+ S    
Sbjct: 1043 SVFLFSPQNKF-RLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQG--SLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGG 1156          

HSP 10 Score: 54.299 bits (129), Expect = 2.197e-6
Identity = 25/80 (31.25%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSF----------WDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY+  DA +           W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1254 NFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1333          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ia (calcium voltage-gated channel subunit alpha1 Ia [Source:ZFIN;Acc:ZDB-GENE-060503-324])

HSP 1 Score: 631.328 bits (1627), Expect = 0.000e+0
Identity = 387/612 (63.24%), Postives = 486/612 (79.41%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            S+++FS  N+FR  CQ I+  K FDY+VL FI  NCIT+A+ERP I   S ERLFLTV+NYIFT IF GEM +KV+S GL IG+QAYL+S WNI+DGFLVF+SL+D+ +++  G   +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SL+PIGNIVLICC FFIIFGILGVQLFKGKFYYC G  ++ +  K+DCL  +  +W + KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAV V+ QP  N+N W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RR+EKKR+  +   Y+ +YS  R  I+++ TS Y D+ I  +I +NV+TM+ E +  P  L+ ALK  NYFFTSTFVLEA+LKL+A G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG L C++  PC G+ RHA FENFGMAFLTLF+++TGDNWNGIMKDTLRE       C  +      I+P+YFV FVL AQFVL+NVVVAVLMKHL++S+K   ++ E++ E++ +L +
Sbjct: 1043 SVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGG--AKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCL-LANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKKRRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSL---QFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMELAQ 1648          

HSP 2 Score: 332.028 bits (850), Expect = 4.047e-91
Identity = 216/395 (54.68%), Postives = 276/395 (69.87%), Query Frame = 1
Query:  400 WFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSS-FYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPP------SIMLLSNNSL--QICKQPARPFTDNLPTKHS-CVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLY 1554
            WFE++S+ +I +NC+TL MYQPC + +C + +CQ++QAFD  I+ FF  EMV+K++A+G  G   Y GDTWNRLD FIV+ G +E       NI  +AIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRCY   N +    GL L + +YQ          ++ FICS  ++NGI+ CSD+P          L   ++L  Q     A P  +   +    CVNWNQYYT C  G  NP++GAINFDNIG+AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LIIIGSFFMINLCLVVIA+QFSETK+RE + M  +R R+ SS+ +  S  EP  CY E+ +L+  I+ K  RR   LY
Sbjct:   12 WFERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDL-QNINFSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTS-GLTLPTPYYQPEED-----DERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEPLVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSS-TLASLAEPGDCYEELFQLVCHILRKARRRSAALY 398          

HSP 3 Score: 290.041 bits (741), Expect = 3.662e-78
Identity = 163/267 (61.05%), Postives = 198/267 (74.16%), Query Frame = 1
Query: 2095 RRPFNKNQNKTKRKNYLSICQQ-------KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE 2871
            +R  + +    K++  L  C+        K+  +V    F RGI+IAILINT+SMGIE+H QP +LT  LE  NIVF+ +F +EMILKL   G   Y++N +N+FDGIIV++ V E+  Q    GLS+LRTFRLLR++KLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM +FGCKF    E      DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS  AALYF+ALMTFGNYVLFNLLVAILVEGF +E
Sbjct:  511 KRSSDSDDTLKKKRTCLGKCKDVWDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCEIIGQS-DGGLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAE 776          

HSP 4 Score: 108.612 bits (270), Expect = 7.087e-23
Identity = 72/280 (25.71%), Postives = 137/280 (48.93%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            ++ R K   IV+ K F+  ++  I +N I++ +E  + P        L + N +FT +FT EMI+K+ + G       YL++ +NI DG +V IS+ +     ++G S+    +LR FRLLR ++ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ +F  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           +  A +YF++ +    + + N+ V ++VE F 
Sbjct:  535 DEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTN--VLEICNIVFTSMFTLEMILKLTAFGFF----EYLRNPYNIFDGIIVIISVCE-----IIGQSDGGLSVLRTFRLLRVIKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKF----------------SLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SPLAALYFVALMTFGNYVLFNLLVAILVEGFQ 774          

HSP 5 Score: 88.5817 bits (218), Expect = 8.987e-17
Identity = 67/266 (25.19%), Postives = 127/266 (47.74%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSW-----------AALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQK 2889
            I TL         I   I +N ++M +E++ QP  L   L++ N  F+  F++E +LKLI  G   + K+ +N  D  IV+L V+ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C   N    C+       F++   + +T+FQ+ T ++WN ++ + +                 + LYF++ +    +VL N++VA+L++       + Q+
Sbjct: 1372 IHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVC---NEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQE 1634          

HSP 6 Score: 75.8702 bits (185), Expect = 6.055e-13
Identity = 55/161 (34.16%), Postives = 92/161 (57.14%), Query Frame = 1
Query: 3901 FDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            F+ + +  I LNC+TL M +P  +I   S+    L   +    I F  EM++K+++ G+  G + YL   WN +D F+V   +++  + +     N  F  +R    +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   13 FERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIF-GRRCYLGDTWNRLDFFIVMAGMVEYSLDL----QNINFSAIRT---VRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 165          

HSP 7 Score: 70.4774 bits (171), Expect = 2.850e-11
Identity = 65/220 (29.55%), Postives = 113/220 (51.36%), Query Frame = 1
Query: 4807 YSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            + + R  +  +V SKYF+  I   I +N I+M  E H  P  L   L++ N  FTS F LE +LKL A G   Y+ + +N  D +IVI+S+   +I + +          + V+R  R+ RV+KL++    +R  L  +++ +  V    +L  L  +IF+ LG+ +FG     + E  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  534 WDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCE-IIGQSDGG--------LSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNMVLYNGM 739          

HSP 8 Score: 67.781 bits (164), Expect = 1.859e-10
Identity = 69/272 (25.37%), Postives = 132/272 (48.53%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLE-----FSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELC--QQGGTS---GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM----------TRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQ 2886
            F   +L  I  N +++ +E   +P  L  +LE      SN +F+ +F+ EM LK++  G+      Y+++ +N+ DG +V + +I++     GG      L +LR  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C + N K    + NFD+L  +++++F + +++ W  ++Y+G+          T  + W  LYFI+ +   ++ + N+ V ++VE F   R  ++
Sbjct: 1066 FDYVVLAFIFSNCITVALE---RPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCLLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQE 1334          

HSP 9 Score: 57.3806 bits (137), Expect = 2.442e-7
Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query:  331 TVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNN 678
            +VF    QNK  R  C  II    F+ + +  I  NCIT+++ +P   Q   + +   +   +YI  A F  EM +K+++MG ++G+  Y   +WN LD F+V V  I+I+ S    
Sbjct: 1043 SVFLFSPQNKF-RLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQG--SLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGG 1156          

HSP 10 Score: 54.299 bits (129), Expect = 2.248e-6
Identity = 25/80 (31.25%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSF----------WDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY+  DA +           W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1254 NFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1333          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Zebrafish
Match: cacna1ia (calcium voltage-gated channel subunit alpha1 Ia [Source:ZFIN;Acc:ZDB-GENE-060503-324])

HSP 1 Score: 631.328 bits (1627), Expect = 0.000e+0
Identity = 387/612 (63.24%), Postives = 486/612 (79.41%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            S+++FS  N+FR  CQ I+  K FDY+VL FI  NCIT+A+ERP I   S ERLFLTV+NYIFT IF GEM +KV+S GL IG+QAYL+S WNI+DGFLVF+SL+D+ +++  G   +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SL+PIGNIVLICC FFIIFGILGVQLFKGKFYYC G  ++ +  K+DCL  +  +W + KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAV V+ QP  N+N W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RR+EKKR+  +   Y+ +YS  R  I+++ TS Y D+ I  +I +NV+TM+ E +  P  L+ ALK  NYFFTSTFVLEA+LKL+A G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG L C++  PC G+ RHA FENFGMAFLTLF+++TGDNWNGIMKDTLRE       C  +      I+P+YFV FVL AQFVL+NVVVAVLMKHL++S+K   ++ E++ E++ +L +
Sbjct: 1109 SVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGG--AKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCL-LANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKKRRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSL---QFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMELAQ 1714          

HSP 2 Score: 347.051 bits (889), Expect = 1.168e-95
Identity = 223/417 (53.48%), Postives = 288/417 (69.06%), Query Frame = 1
Query:  334 VFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSS-FYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPP------SIMLLSNNSL--QICKQPARPFTDNLPTKHS-CVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLY 1554
            VF    Q   PR+WC++++   WFE++S+ +I +NC+TL MYQPC + +C + +CQ++QAFD  I+ FF  EMV+K++A+G  G   Y GDTWNRLD FIV+ G +E       NI  +AIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRCY   N +    GL L + +YQ          ++ FICS  ++NGI+ CSD+P          L   ++L  Q     A P  +   +    CVNWNQYYT C  G  NP++GAINFDNIG+AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LIIIGSFFMINLCLVVIA+QFSETK+RE + M  +R R+ SS+ +  S  EP  CY E+ +L+  I+ K  RR   LY
Sbjct:   59 VFFCLKQTTCPRNWCIRMV---WFERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDL-QNINFSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTS-GLTLPTPYYQPEED-----DERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEPLVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSS-TLASLAEPGDCYEELFQLVCHILRKARRRSAALY 464          

HSP 3 Score: 290.426 bits (742), Expect = 2.825e-78
Identity = 163/267 (61.05%), Postives = 198/267 (74.16%), Query Frame = 1
Query: 2095 RRPFNKNQNKTKRKNYLSICQQ-------KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE 2871
            +R  + +    K++  L  C+        K+  +V    F RGI+IAILINT+SMGIE+H QP +LT  LE  NIVF+ +F +EMILKL   G   Y++N +N+FDGIIV++ V E+  Q    GLS+LRTFRLLR++KLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM +FGCKF    E      DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS  AALYF+ALMTFGNYVLFNLLVAILVEGF +E
Sbjct:  577 KRSSDSDDTLKKKRTCLGKCKDVWDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCEIIGQS-DGGLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAE 842          

HSP 4 Score: 108.997 bits (271), Expect = 6.560e-23
Identity = 72/280 (25.71%), Postives = 137/280 (48.93%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            ++ R K   IV+ K F+  ++  I +N I++ +E  + P        L + N +FT +FT EMI+K+ + G       YL++ +NI DG +V IS+ +     ++G S+    +LR FRLLR ++ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ +F  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           +  A +YF++ +    + + N+ V ++VE F 
Sbjct:  601 DEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTN--VLEICNIVFTSMFTLEMILKLTAFGFF----EYLRNPYNIFDGIIVIISVCE-----IIGQSDGGLSVLRTFRLLRVIKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKF----------------SLKTEAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SPLAALYFVALMTFGNYVLFNLLVAILVEGFQ 840          

HSP 5 Score: 88.9669 bits (219), Expect = 7.367e-17
Identity = 67/266 (25.19%), Postives = 127/266 (47.74%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSW-----------AALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQK 2889
            I TL         I   I +N ++M +E++ QP  L   L++ N  F+  F++E +LKLI  G   + K+ +N  D  IV+L V+ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C   N    C+       F++   + +T+FQ+ T ++WN ++ + +                 + LYF++ +    +VL N++VA+L++       + Q+
Sbjct: 1438 IHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYFFTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVC---NEDYPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPPGEYTCNPSLQFISPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQE 1700          

HSP 6 Score: 75.8702 bits (185), Expect = 5.959e-13
Identity = 55/161 (34.16%), Postives = 92/161 (57.14%), Query Frame = 1
Query: 3901 FDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            F+ + +  I LNC+TL M +P  +I   S+    L   +    I F  EM++K+++ G+  G + YL   WN +D F+V   +++  + +     N  F  +R    +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   79 FERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKMVALGIF-GRRCYLGDTWNRLDFFIVMAGMVEYSLDL----QNINFSAIRT---VRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 231          

HSP 7 Score: 70.4774 bits (171), Expect = 2.973e-11
Identity = 65/220 (29.55%), Postives = 113/220 (51.36%), Query Frame = 1
Query: 4807 YSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            + + R  +  +V SKYF+  I   I +N I+M  E H  P  L   L++ N  FTS F LE +LKL A G   Y+ + +N  D +IVI+S+   +I + +          + V+R  R+ RV+KL++    +R  L  +++ +  V    +L  L  +IF+ LG+ +FG     + E  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  600 WDEIRVKLWGIVESKYFNRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIFDGIIVIISVCE-IIGQSDGG--------LSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTEAGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNMVLYNGM 805          

HSP 8 Score: 67.781 bits (164), Expect = 2.126e-10
Identity = 69/272 (25.37%), Postives = 132/272 (48.53%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLE-----FSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELC--QQGGTS---GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM----------TRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQ 2886
            F   +L  I  N +++ +E   +P  L  +LE      SN +F+ +F+ EM LK++  G+      Y+++ +N+ DG +V + +I++     GG      L +LR  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C + N K    + NFD+L  +++++F + +++ W  ++Y+G+          T  + W  LYFI+ +   ++ + N+ V ++VE F   R  ++
Sbjct: 1132 FDYVVLAFIFSNCITVALE---RPKILQGSLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCLGLDVKNITNKSDCLLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQE 1400          

HSP 9 Score: 57.3806 bits (137), Expect = 2.742e-7
Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = 1
Query:  331 TVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNN 678
            +VF    QNK  R  C  II    F+ + +  I  NCIT+++ +P   Q   + +   +   +YI  A F  EM +K+++MG ++G+  Y   +WN LD F+V V  I+I+ S    
Sbjct: 1109 SVFLFSPQNKF-RLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQG--SLERLFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSMAGG 1222          

HSP 10 Score: 54.299 bits (129), Expect = 2.319e-6
Identity = 25/80 (31.25%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSF----------WDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY+  DA +           W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1320 NFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAVDQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1399          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: efhd1 (EF-hand domain family member D1 [Source:Xenbase;Acc:XB-GENE-5738953])

HSP 1 Score: 645.58 bits (1664), Expect = 0.000e+0
Identity = 403/670 (60.15%), Postives = 509/670 (75.97%), Query Frame = 1
Query: 3739 EDP--DEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTL 5727
            EDP   E+D+E   C  + K     K  + K +   SLY+FS  NQFR  CQ ++  K FD++VL FI LNCIT+A+ERP I P+S ER+FL+V+NYIFT IF  EM IKV++ G + G+  YL+S WN++DG LVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  +++C + ++ +W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAV  ++QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLEKKR+  +   Y+  YS  R  I+++ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EAL KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C++ NPC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE C S +   +NC     +I+P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++  +      + Q +D   + N L
Sbjct: 1210 EDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTR-ARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRRPYYAEYSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE-CNSDD---KNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMSRDG-----DFQRRDGSGSRNLL 1869          

HSP 2 Score: 407.142 bits (1045), Expect = 4.588e-114
Identity = 233/445 (52.36%), Postives = 311/445 (69.89%), Query Frame = 1
Query:  298 NESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHC 1632
             E + Y     TVF    Q   PR+WCL+++  P +   SM +I +NC+TL M+QPC D NC + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWN LD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+++ N    +    L  +YQ++     +A D  +ICS++++NG+LKC DIPP +              +  +  + P+++SCVN NQYY  C+PGE NP++GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K I  I  K+ RR ++LYN  Q +  K  Q N     + + + C
Sbjct:   63 GEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNP-YPLHSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLE-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVD-----DAEDNPYICSSNRDNGMLKCQDIPPKM------------DCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKC 488          

HSP 3 Score: 344.739 bits (883), Expect = 1.094e-94
Identity = 195/319 (61.13%), Postives = 238/319 (74.61%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE------RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSP 3096
            ++K +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F +EM+LKL+  GI  YIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF  T +  +   DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        +E K   N+ + L+K     K+LR  + +  +L +  N         + + S  PP+I+ TAATPMPTP+SSP
Sbjct:  752 RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQS-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKL----KELRAAELKMYSLAVTPNG------HLDPRSSMPPPIIMRTAATPMPTPKSSP 1059          

HSP 4 Score: 116.316 bits (290), Expect = 4.630e-25
Identity = 73/285 (25.61%), Postives = 141/285 (49.47%), Query Frame = 1
Query: 3841 VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            V+ +  +F  + + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G+      Y+K+ +NI DG +V IS+ +     ++G S+    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF +                     E    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  742 VYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNAL--EISNIVFTSMFALEMLLKLLAFGIF----QYIKNPYNIFDGIIVVISVWE-----IIGQSDGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTW----------------TADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 986          

HSP 5 Score: 106.686 bits (265), Expect = 4.146e-22
Identity = 99/332 (29.82%), Postives = 157/332 (47.29%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHS-KER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG---------------------KFYYC-----DGPHL------------QAVKTKNDCLKYSQNEWK--------NQKY---------------NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            +  I LNC+TL M +P    +   ER  +      +IF   F  EM+IK+++ G+  G + YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G                     + YY      D P++            Q +  K DC     +++         NQ Y               NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  102 MLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAF-FAVEMVIKMVALGIF-GQKCYLGDTWNWLDFFIVMAGMLEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 414          

HSP 6 Score: 86.2705 bits (212), Expect = 5.863e-16
Identity = 55/201 (27.36%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            I   I IN ++M +E++ QP  L + L++ N VF+I+F+ E + KL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+F++ T ++WN ++ + +   +S
Sbjct: 1590 ITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNS 1790          

HSP 7 Score: 76.6406 bits (187), Expect = 5.930e-13
Identity = 67/216 (31.02%), Postives = 117/216 (54.17%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIM 5439
            L WE ++     +  +V SKYF+  I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G+ +YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG       +    +    +F++   A +T+F+I T ++WN ++
Sbjct:  744 LLWEEFTV---RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWE-IIGQSDGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVL 947          

HSP 8 Score: 74.3294 bits (181), Expect = 2.904e-12
Identity = 64/281 (22.78%), Postives = 133/281 (47.33%), Query Frame = 1
Query: 2149 ICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGILG----YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCDRK---------NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +CQ+ I   +    F   +L+ I +N +++ +E     P  L +  L  SN +F+ +F++EM +K++  G +     Y+++ +N+ DGI+V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E        N+  C R          NFD+L  +++++F + +++ W  ++Y+G+   ++          W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1259 MCQKVIAHKM----FDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1535          

HSP 9 Score: 63.1586 bits (152), Expect = 6.571e-9
Identity = 37/117 (31.62%), Postives = 62/117 (52.99%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D N  + +   +   +YI  A F  EM IK++A+GF+ G+  Y   +WN LD  +V V  I+I+ S  +  G
Sbjct: 1247 LYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPN--SLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGG 1361          

HSP 10 Score: 54.6842 bits (130), Expect = 2.260e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1459 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1538          

HSP 11 Score: 53.9138 bits (128), Expect = 4.070e-6
Identity = 24/75 (32.00%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R +TE
Sbjct:  923 NFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTE 997          

HSP 12 Score: 53.5286 bits (127), Expect = 6.041e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  343 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQR 420          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: efhd1 (EF-hand domain family member D1 [Source:Xenbase;Acc:XB-GENE-5738953])

HSP 1 Score: 642.884 bits (1657), Expect = 0.000e+0
Identity = 389/611 (63.67%), Postives = 487/611 (79.71%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDL 5658
            SLY+FS  NQFR  CQ ++  K FD++VL FI LNCIT+A+ERP I P+S ER+FL+V+NYIFT IF  EM IKV++ G + G+  YL+S WN++DG LVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  +++C + ++ +W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAV  ++QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLEKKR+  +   Y+  YS  R  I+++ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EAL KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C++ NPC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE   +S+D  +NC     +I+P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++
Sbjct: 1155 SLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTR-ARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRRPYYAEYSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE--CNSDD--KNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEM 1760          

HSP 2 Score: 404.831 bits (1039), Expect = 1.783e-113
Identity = 236/456 (51.75%), Postives = 313/456 (68.64%), Query Frame = 1
Query:  265 QQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHC 1632
            Q    G      E + Y     TVF    Q   PR+WCL+++  P +   SM +I +NC+TL M+QPC D NC + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWN LD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+++ N  F  P      +YQ++     +A D  +ICS++++NG+LKC DIPP +              +  +  + P+++SCVN NQYY  C+PGE NP++GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K I  I  K+ RR ++LYN  Q +  K  Q N     + + + C
Sbjct:   22 QGREQGMGSEEGEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNP-YPLHSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLE-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTN--FVMP------YYQVD-----DAEDNPYICSSNRDNGMLKCQDIPPKM------------DCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKC 450          

HSP 3 Score: 334.724 bits (857), Expect = 1.155e-91
Identity = 188/313 (60.06%), Postives = 231/313 (73.80%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSP 3096
            ++K +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F +EM+LKL+  GI  YIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF  T +  +   DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E                  R++ ++ +  + +  +L +  N         + + S  PP+I+ TAATPMPTP+SSP
Sbjct:  689 RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQS-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEG--------------DATRSDTEEDKASELKMYSLAVTPNG------HLDPRSSMPPPIIMRTAATPMPTPKSSP 980          

HSP 4 Score: 115.546 bits (288), Expect = 7.976e-25
Identity = 73/285 (25.61%), Postives = 141/285 (49.47%), Query Frame = 1
Query: 3841 VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            V+ +  +F  + + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G+      Y+K+ +NI DG +V IS+ +     ++G S+    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF +                     E    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  679 VYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNAL--EISNIVFTSMFALEMLLKLLAFGIF----QYIKNPYNIFDGIIVVISVWE-----IIGQSDGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTW----------------TADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 923          

HSP 5 Score: 108.612 bits (270), Expect = 1.008e-22
Identity = 99/324 (30.56%), Postives = 156/324 (48.15%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHS-KER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF-------------YYC-----DGPHL------------QAVKTKNDCLKYSQNEWK--------NQKY---------------NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            +  I LNC+TL M +P    +   ER  +      +IF   F  EM+IK+++ G+  G + YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G               YY      D P++            Q +  K DC     +++         NQ Y               NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:   72 MLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAF-FAVEMVIKMVALGIF-GQKCYLGDTWNWLDFFIVMAGMLEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 376          

HSP 6 Score: 85.5001 bits (210), Expect = 9.910e-16
Identity = 55/201 (27.36%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            I   I IN ++M +E++ QP  L + L++ N VF+I+F+ E + KL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+F++ T ++WN ++ + +   +S
Sbjct: 1499 ITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNS 1699          

HSP 7 Score: 76.6406 bits (187), Expect = 5.669e-13
Identity = 67/220 (30.45%), Postives = 119/220 (54.09%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            L WE ++     +  +V SKYF+  I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G+ +YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG       +    +    +F++   A +T+F+I T ++WN ++ + +
Sbjct:  681 LLWEEFTV---RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWE-IIGQSDGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGM 888          

HSP 8 Score: 73.559 bits (179), Expect = 4.411e-12
Identity = 64/281 (22.78%), Postives = 133/281 (47.33%), Query Frame = 1
Query: 2149 ICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGILG----YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCDRK---------NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +CQ+ I   +    F   +L+ I +N +++ +E     P  L +  L  SN +F+ +F++EM +K++  G +     Y+++ +N+ DGI+V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E        N+  C R          NFD+L  +++++F + +++ W  ++Y+G+   ++          W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1168 MCQKVIAHKM----FDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1444          

HSP 9 Score: 62.7734 bits (151), Expect = 7.903e-9
Identity = 37/117 (31.62%), Postives = 62/117 (52.99%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D N  + +   +   +YI  A F  EM IK++A+GF+ G+  Y   +WN LD  +V V  I+I+ S  +  G
Sbjct: 1156 LYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPN--SLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGG 1270          

HSP 10 Score: 54.6842 bits (130), Expect = 2.524e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1368 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1447          

HSP 11 Score: 53.9138 bits (128), Expect = 4.250e-6
Identity = 24/75 (32.00%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R +TE
Sbjct:  860 NFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTE 934          

HSP 12 Score: 53.1434 bits (126), Expect = 6.805e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  305 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQR 382          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: efhd1 (EF-hand domain family member D1 [Source:Xenbase;Acc:XB-GENE-5738953])

HSP 1 Score: 642.499 bits (1656), Expect = 0.000e+0
Identity = 393/656 (59.91%), Postives = 497/656 (75.76%), Query Frame = 1
Query: 3739 EDP--DEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ--------XXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLD 5661
            EDP   E+D+E   C  + K     K  + K +   SLY+FS  NQFR  CQ ++  K FD++VL FI LNCIT+A+ERP I P+S ER+FL+V+NYIFT IF  EM IKV++ G + G+  YL+S WN++DG LVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  +++C + ++ +W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAV  ++QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++Q        E++   R   KR  +     +  R P Y E YS  R  I+++ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EAL KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C++ NPC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE   +S+D  +NC     +I+P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++D
Sbjct: 1202 EDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTR-ARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKALYMSEAQRRPYYAE-YSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE--CNSDD--KNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMD 1851          

HSP 2 Score: 407.527 bits (1046), Expect = 3.473e-114
Identity = 233/445 (52.36%), Postives = 311/445 (69.89%), Query Frame = 1
Query:  298 NESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHC 1632
             E + Y     TVF    Q   PR+WCL+++  P +   SM +I +NC+TL M+QPC D NC + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWN LD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+++ N    +    L  +YQ++     +A D  +ICS++++NG+LKC DIPP +              +  +  + P+++SCVN NQYY  C+PGE NP++GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K I  I  K+ RR ++LYN  Q +  K  Q N     + + + C
Sbjct:   57 GEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNP-YPLHSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLE-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVD-----DAEDNPYICSSNRDNGMLKCQDIPPKM------------DCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKC 482          

HSP 3 Score: 344.739 bits (883), Expect = 1.004e-94
Identity = 195/319 (61.13%), Postives = 238/319 (74.61%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE------RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSP 3096
            ++K +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F +EM+LKL+  GI  YIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF  T +  +   DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        +E K   N+ + L+K     K+LR  + +  +L +  N         + + S  PP+I+ TAATPMPTP+SSP
Sbjct:  746 RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQS-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKL----KELRAAELKMYSLAVTPNG------HLDPRSSMPPPIIMRTAATPMPTPKSSP 1053          

HSP 4 Score: 116.316 bits (290), Expect = 4.603e-25
Identity = 73/285 (25.61%), Postives = 141/285 (49.47%), Query Frame = 1
Query: 3841 VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            V+ +  +F  + + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G+      Y+K+ +NI DG +V IS+ +     ++G S+    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF +                     E    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  736 VYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNAL--EISNIVFTSMFALEMLLKLLAFGIF----QYIKNPYNIFDGIIVVISVWE-----IIGQSDGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTW----------------TADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 980          

HSP 5 Score: 107.071 bits (266), Expect = 3.635e-22
Identity = 99/332 (29.82%), Postives = 157/332 (47.29%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHS-KER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG---------------------KFYYC-----DGPHL------------QAVKTKNDCLKYSQNEWK--------NQKY---------------NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            +  I LNC+TL M +P    +   ER  +      +IF   F  EM+IK+++ G+  G + YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G                     + YY      D P++            Q +  K DC     +++         NQ Y               NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:   96 MLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAF-FAVEMVIKMVALGIF-GQKCYLGDTWNWLDFFIVMAGMLEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 408          

HSP 6 Score: 86.2705 bits (212), Expect = 5.791e-16
Identity = 55/201 (27.36%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            I   I IN ++M +E++ QP  L + L++ N VF+I+F+ E + KL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+F++ T ++WN ++ + +   +S
Sbjct: 1589 ITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNS 1789          

HSP 7 Score: 76.6406 bits (187), Expect = 4.830e-13
Identity = 67/220 (30.45%), Postives = 119/220 (54.09%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            L WE ++     +  +V SKYF+  I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G+ +YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG       +    +    +F++   A +T+F+I T ++WN ++ + +
Sbjct:  738 LLWEEFTV---RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWE-IIGQSDGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGM 945          

HSP 8 Score: 74.3294 bits (181), Expect = 2.509e-12
Identity = 64/281 (22.78%), Postives = 133/281 (47.33%), Query Frame = 1
Query: 2149 ICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGILG----YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCDRK---------NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +CQ+ I   +    F   +L+ I +N +++ +E     P  L +  L  SN +F+ +F++EM +K++  G +     Y+++ +N+ DGI+V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E        N+  C R          NFD+L  +++++F + +++ W  ++Y+G+   ++          W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1251 MCQKVIAHKM----FDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1527          

HSP 9 Score: 63.5438 bits (153), Expect = 5.876e-9
Identity = 37/117 (31.62%), Postives = 62/117 (52.99%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D N  + +   +   +YI  A F  EM IK++A+GF+ G+  Y   +WN LD  +V V  I+I+ S  +  G
Sbjct: 1239 LYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPN--SLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGG 1353          

HSP 10 Score: 54.6842 bits (130), Expect = 2.274e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1451 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1530          

HSP 11 Score: 53.9138 bits (128), Expect = 4.062e-6
Identity = 24/75 (32.00%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R +TE
Sbjct:  917 NFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTE 991          

HSP 12 Score: 53.5286 bits (127), Expect = 6.131e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  337 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQR 414          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: efhd1 (EF-hand domain family member D1 [Source:Xenbase;Acc:XB-GENE-5738953])

HSP 1 Score: 641.728 bits (1654), Expect = 0.000e+0
Identity = 396/678 (58.41%), Postives = 503/678 (74.19%), Query Frame = 1
Query: 3739 EDP--DEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ--------XXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTL 5727
            EDP   E+D+E   C  + K     K  + K +   SLY+FS  NQFR  CQ ++  K FD++VL FI LNCIT+A+ERP I P+S ER+FL+V+NYIFT IF  EM IKV++ G + G+  YL+S WN++DG LVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  +++C + ++ +W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAV  ++QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++Q        E++   R   KR  +     +  R P Y E YS  R  I+++ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EAL KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C++ NPC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE C S +   +NC     +I+P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++  +      + Q +D   + N L
Sbjct: 1212 EDPLEYEDDIEDSYCFRIRKMLEPYKPTWCKTHEDWSLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTR-ARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKALYMSEAQRRPYYAE-YSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE-CNSDD---KNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEMSRDG-----DFQRRDGSGSRNLL 1878          

HSP 2 Score: 407.527 bits (1046), Expect = 3.009e-114
Identity = 233/445 (52.36%), Postives = 311/445 (69.89%), Query Frame = 1
Query:  298 NESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHC 1632
             E + Y     TVF    Q   PR+WCL+++  P +   SM +I +NC+TL M+QPC D NC + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWN LD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+++ N    +    L  +YQ++     +A D  +ICS++++NG+LKC DIPP +              +  +  + P+++SCVN NQYY  C+PGE NP++GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K I  I  K+ RR ++LYN  Q +  K  Q N     + + + C
Sbjct:   67 GEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNP-YPLHSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLE-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVD-----DAEDNPYICSSNRDNGMLKCQDIPPKM------------DCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKC 492          

HSP 3 Score: 345.125 bits (884), Expect = 8.368e-95
Identity = 195/319 (61.13%), Postives = 238/319 (74.61%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE------RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSP 3096
            ++K +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F +EM+LKL+  GI  YIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF  T +  +   DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        +E K   N+ + L+K     K+LR  + +  +L +  N         + + S  PP+I+ TAATPMPTP+SSP
Sbjct:  756 RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQS-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTEEDKASGNFDEDLEKL----KELRAAELKMYSLAVTPNG------HLDPRSSMPPPIIMRTAATPMPTPKSSP 1063          

HSP 4 Score: 116.316 bits (290), Expect = 4.679e-25
Identity = 73/285 (25.61%), Postives = 141/285 (49.47%), Query Frame = 1
Query: 3841 VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            V+ +  +F  + + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G+      Y+K+ +NI DG +V IS+ +     ++G S+    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF +                     E    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  746 VYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNAL--EISNIVFTSMFALEMLLKLLAFGIF----QYIKNPYNIFDGIIVVISVWE-----IIGQSDGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTW----------------TADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 990          

HSP 5 Score: 106.686 bits (265), Expect = 3.727e-22
Identity = 99/332 (29.82%), Postives = 157/332 (47.29%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHS-KER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG---------------------KFYYC-----DGPHL------------QAVKTKNDCLKYSQNEWK--------NQKY---------------NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            +  I LNC+TL M +P    +   ER  +      +IF   F  EM+IK+++ G+  G + YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G                     + YY      D P++            Q +  K DC     +++         NQ Y               NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  106 MLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAF-FAVEMVIKMVALGIF-GQKCYLGDTWNWLDFFIVMAGMLEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFVMSYNFTPLRPYYQVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 418          

HSP 6 Score: 86.2705 bits (212), Expect = 5.888e-16
Identity = 55/201 (27.36%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            I   I IN ++M +E++ QP  L + L++ N VF+I+F+ E + KL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+F++ T ++WN ++ + +   +S
Sbjct: 1599 ITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNS 1799          

HSP 7 Score: 76.6406 bits (187), Expect = 4.994e-13
Identity = 67/216 (31.02%), Postives = 117/216 (54.17%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIM 5439
            L WE ++     +  +V SKYF+  I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G+ +YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG       +    +    +F++   A +T+F+I T ++WN ++
Sbjct:  748 LLWEEFTV---RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWE-IIGQSDGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVL 951          

HSP 8 Score: 74.3294 bits (181), Expect = 2.706e-12
Identity = 64/281 (22.78%), Postives = 133/281 (47.33%), Query Frame = 1
Query: 2149 ICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGILG----YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCDRK---------NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +CQ+ I   +    F   +L+ I +N +++ +E     P  L +  L  SN +F+ +F++EM +K++  G +     Y+++ +N+ DGI+V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E        N+  C R          NFD+L  +++++F + +++ W  ++Y+G+   ++          W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1261 MCQKVIAHKM----FDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1537          

HSP 9 Score: 63.5438 bits (153), Expect = 5.875e-9
Identity = 37/117 (31.62%), Postives = 62/117 (52.99%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D N  + +   +   +YI  A F  EM IK++A+GF+ G+  Y   +WN LD  +V V  I+I+ S  +  G
Sbjct: 1249 LYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPN--SLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGG 1363          

HSP 10 Score: 55.0694 bits (131), Expect = 2.181e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1461 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1540          

HSP 11 Score: 53.9138 bits (128), Expect = 3.927e-6
Identity = 24/75 (32.00%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R +TE
Sbjct:  927 NFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTE 1001          

HSP 12 Score: 53.5286 bits (127), Expect = 5.731e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  347 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQR 424          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Xenopus
Match: efhd1 (EF-hand domain family member D1 [Source:Xenbase;Acc:XB-GENE-5738953])

HSP 1 Score: 637.491 bits (1643), Expect = 0.000e+0
Identity = 382/619 (61.71%), Postives = 481/619 (77.71%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ--------XXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDL 5658
            SLY+FS  NQFR  CQ ++  K FD++VL FI LNCIT+A+ERP I P+S ER+FL+V+NYIFT IF  EM IKV++ G + G+  YL+S WN++DG LVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  +++C + ++ +W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAV  ++QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++Q        E++   R   KR  +     +  R P Y E YS  R  I+++ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EAL KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C++ NPC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE   +S+D  +NC     +I+P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++
Sbjct: 1181 SLYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTR-ARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRSKALYMSEAQRRPYYAE-YSPARLYIHTMCTSHYLDLFITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE--CNSDD--KNCLTYLPVISPVYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIEMEM 1793          

HSP 2 Score: 397.512 bits (1020), Expect = 4.025e-111
Identity = 232/445 (52.13%), Postives = 307/445 (68.99%), Query Frame = 1
Query:  298 NESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHC 1632
             E + Y     TVF    Q   PR+WCL+++  P +   SM +I +NC+TL M+QPC D NC + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWN LD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+++ N            F  I+     +A D  +ICS++++NG+LKC DIPP +              +  +  + P+++SCVN NQYY  C+PGE NP++GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K I  I  K+ RR ++LYN  Q +  K  Q N     + + + C
Sbjct:   67 GEQVPYPALAPTVFFCLSQTTRPRNWCLRLVCNP-YPLHSMLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAFFAVEMVIKMVALGIFGQKCYLGDTWNWLDFFIVMAGMLE-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTN------------FLPISVD---DAEDNPYICSSNRDNGMLKCQDIPPKM------------DCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQRARYLSNDSTIASFSEPGSCYEELLKYICHIFRKVKRRTLRLYNNWQNKRRKKVQPNSNTNSQGRHRKC 482          

HSP 3 Score: 327.405 bits (838), Expect = 2.246e-89
Identity = 187/314 (59.55%), Postives = 222/314 (70.70%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGE-NQKISSQPPVIIHTAATPMPTPRSSP 3096
            ++K +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F +EM+LKL+  GI  YIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF  T +  +   DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E                                        S+     G  + + S  PP+I+ TAATPMPTP+SSP
Sbjct:  706 RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWEIIGQS-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEMY--------------------------------------SLAVTPNGHLDPRSSMPPPIIMRTAATPMPTPKSSP 980          

HSP 4 Score: 115.931 bits (289), Expect = 6.594e-25
Identity = 73/285 (25.61%), Postives = 141/285 (49.47%), Query Frame = 1
Query: 3841 VFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            V+ +  +F  + + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G+      Y+K+ +NI DG +V IS+ +     ++G S+    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF +                     E    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  696 VYLLWEEFTVRLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNAL--EISNIVFTSMFALEMLLKLLAFGIF----QYIKNPYNIFDGIIVVISVWE-----IIGQSDGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTW----------------TADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 940          

HSP 5 Score: 108.227 bits (269), Expect = 1.619e-22
Identity = 98/322 (30.43%), Postives = 155/322 (48.14%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHS-KER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFY-YC---------------DGPHL------------QAVKTKNDCLKYSQNEWK--------NQKY---------------NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            +  I LNC+TL M +P    +   ER  +      +IF   F  EM+IK+++ G+  G + YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G     C               D P++            Q +  K DC     +++         NQ Y               NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  106 MLVILLNCVTLGMFQPCEDTNCMSERCNILEAFDGFIFAF-FAVEMVIKMVALGIF-GQKCYLGDTWNWLDFFIVMAGMLEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMDTNFLPISVDDAEDNPYICSSNRDNGMLKCQDIPPKMDCSHVYYSDFPSRNSCVNINQYYNVCKPGEFNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 408          

HSP 6 Score: 86.2705 bits (212), Expect = 6.977e-16
Identity = 55/201 (27.36%), Postives = 106/201 (52.74%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            I   I IN ++M +E++ QP  L + L++ N VF+I+F+ E + KL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+F++ T ++WN ++ + +   +S
Sbjct: 1532 ITCIICINVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEALFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNEENPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRECNS 1732          

HSP 7 Score: 77.0258 bits (188), Expect = 4.564e-13
Identity = 67/220 (30.45%), Postives = 119/220 (54.09%), Query Frame = 1
Query: 4792 LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            L WE ++     +  +V SKYF+  I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G+ +YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG       +    +    +F++   A +T+F+I T ++WN ++ + +
Sbjct:  698 LLWEEFTV---RLKRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNALEISNIVFTSMFALEMLLKLLAFGIFQYIKNPYNIFDGIIVVISVWE-IIGQSDGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLVKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFTWTADTGETVKDRKNFDSLLWAIVTVFQILTQEDWNMVLYNGM 905          

HSP 8 Score: 74.3294 bits (181), Expect = 2.830e-12
Identity = 64/281 (22.78%), Postives = 133/281 (47.33%), Query Frame = 1
Query: 2149 ICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGILG----YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCDRK---------NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +CQ+ I   +    F   +L+ I +N +++ +E     P  L +  L  SN +F+ +F++EM +K++  G +     Y+++ +N+ DGI+V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E        N+  C R          NFD+L  +++++F + +++ W  ++Y+G+   ++          W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1194 MCQKVIAHKM----FDHVVLVFIFLNCITIALERPDIDPNSLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLETRNITNRSECTRARYKWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1470          

HSP 9 Score: 62.7734 bits (151), Expect = 9.931e-9
Identity = 37/117 (31.62%), Postives = 62/117 (52.99%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D N  + +   +   +YI  A F  EM IK++A+GF+ G+  Y   +WN LD  +V V  I+I+ S  +  G
Sbjct: 1182 LYMFSPQNQFRMMCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPN--SLERIFLSVSNYIFTAIFVLEMAIKVVALGFVSGENTYLQSSWNVLDGILVFVSIIDIVVSLASAGG 1296          

HSP 10 Score: 54.299 bits (129), Expect = 2.940e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1394 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVATDVQPVKNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1473          

HSP 11 Score: 53.5286 bits (127), Expect = 5.663e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  337 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMQEQR 414          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1h (calcium channel, voltage-dependent, T type, alpha 1H subunit [Source:MGI Symbol;Acc:MGI:1928842])

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 399/646 (61.76%), Postives = 497/646 (76.93%), Query Frame = 1
Query: 3814 AKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE----EAKELAREKQLKDEENNENTLI 5730
            + R   +LY+F   N+ R  CQ ++  K FD++VL FI LNCIT+A+ERP I P S ER FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + + TK +C   +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVG++ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I LNVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   + ED     C+S +  ++P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++ +    +    A+E Q   E +  N L+
Sbjct: 1278 SSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAEC-HAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CTREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQ-GTEPDTPNLLV 1916          

HSP 2 Score: 377.096 bits (967), Expect = 7.836e-105
Identity = 241/469 (51.39%), Postives = 312/469 (66.52%), Query Frame = 1
Query:  199 RETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK 1581
            RE   G+ S   L  +S    C  +       P   + Y    ATVF    Q   PR WCL+++  PWFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP     D    +++C+NWNQYY  C+ GE NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K
Sbjct:   38 REEQRGSGSSV-LAPESPGTECGADLGADEEQP---VPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMME-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQA--EDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRK 488          

HSP 3 Score: 337.806 bits (865), Expect = 1.066e-92
Identity = 190/324 (58.64%), Postives = 234/324 (72.22%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELV 3111
            K++ +V+   F RGI+ AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGI+V++ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V++RT+DNVATF  LL+LFIFIFSILGM LFGCKF    ++  T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   +  +   K       LR +Q     +  L  +      G     S  PP+I HTAATPMPTP+SSP+ ++ 
Sbjct:  782 KLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDK-------LRDVQATEMKMYSLAVTPNGHLEGRG---SLPPPLITHTAATPMPTPKSSPHLDMA 1094          

HSP 4 Score: 114.39 bits (285), Expect = 1.586e-24
Identity = 73/278 (26.26%), Postives = 138/278 (49.64%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  779 FSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNAL--EISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIVVIISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------SLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1016          

HSP 5 Score: 82.8037 bits (203), Expect = 5.314e-15
Identity = 64/247 (25.91%), Postives = 121/247 (48.99%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM---TRTSSWAALYFIAL 2802
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   TR       Y  AL
Sbjct: 1603 PYYADYSHTRRSIH-SLCTSHYLDLF--------ITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPAL 1840          

HSP 6 Score: 82.0333 bits (201), Expect = 9.177e-15
Identity = 61/173 (35.26%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVMAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 75.0998 bits (183), Expect = 1.308e-12
Identity = 71/224 (31.70%), Postives = 119/224 (53.12%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W ++S K RR    +V SKYF+  I   I +N ++M  E+H  P  L  AL++ N  FTS F LE LLKL+A G   YI + +N  D ++VI+S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  776 WASFSSKLRR----IVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 981          

HSP 8 Score: 70.4774 bits (171), Expect = 3.085e-11
Identity = 65/282 (23.05%), Postives = 138/282 (48.94%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSIL-------RTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N L +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F++EM++K++  G+L     Y+++ +N+ DG++V++ ++++     ++G + +       R  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1291 QNRLRVSCQKV---IAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1569          

HSP 9 Score: 63.929 bits (154), Expect = 2.676e-9
Identity = 33/118 (27.97%), Postives = 64/118 (54.24%), Query Frame = 1
Query:  334 VFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
              ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+ +  +  G
Sbjct: 1284 ALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERP--DIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGG 1399          

HSP 10 Score: 55.8398 bits (133), Expect = 9.279e-7
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 1
Query: 4462 NQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  356 NGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 431          

HSP 11 Score: 54.6842 bits (130), Expect = 1.848e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1497 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1576          

HSP 12 Score: 52.7582 bits (125), Expect = 7.150e-6
Identity = 23/75 (30.67%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R +T+
Sbjct:  953 NFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTD 1027          

HSP 13 Score: 52.373 bits (124), Expect = 8.821e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  360 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQR 437          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1h (calcium channel, voltage-dependent, T type, alpha 1H subunit [Source:MGI Symbol;Acc:MGI:1928842])

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 399/646 (61.76%), Postives = 497/646 (76.93%), Query Frame = 1
Query: 3814 AKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE----EAKELAREKQLKDEENNENTLI 5730
            + R   +LY+F   N+ R  CQ ++  K FD++VL FI LNCIT+A+ERP I P S ER FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + + TK +C   +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVG++ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I LNVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   + ED     C+S +  ++P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++ +    +    A+E Q   E +  N L+
Sbjct: 1278 SSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAEC-HAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CTREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQ-GTEPDTPNLLV 1916          

HSP 2 Score: 377.096 bits (967), Expect = 7.836e-105
Identity = 241/469 (51.39%), Postives = 312/469 (66.52%), Query Frame = 1
Query:  199 RETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK 1581
            RE   G+ S   L  +S    C  +       P   + Y    ATVF    Q   PR WCL+++  PWFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP     D    +++C+NWNQYY  C+ GE NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K
Sbjct:   38 REEQRGSGSSV-LAPESPGTECGADLGADEEQP---VPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMME-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQA--EDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRK 488          

HSP 3 Score: 337.806 bits (865), Expect = 1.066e-92
Identity = 190/324 (58.64%), Postives = 234/324 (72.22%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELV 3111
            K++ +V+   F RGI+ AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGI+V++ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V++RT+DNVATF  LL+LFIFIFSILGM LFGCKF    ++  T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   +  +   K       LR +Q     +  L  +      G     S  PP+I HTAATPMPTP+SSP+ ++ 
Sbjct:  782 KLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDK-------LRDVQATEMKMYSLAVTPNGHLEGRG---SLPPPLITHTAATPMPTPKSSPHLDMA 1094          

HSP 4 Score: 114.39 bits (285), Expect = 1.586e-24
Identity = 73/278 (26.26%), Postives = 138/278 (49.64%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  779 FSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNAL--EISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIVVIISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------SLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1016          

HSP 5 Score: 82.8037 bits (203), Expect = 5.314e-15
Identity = 64/247 (25.91%), Postives = 121/247 (48.99%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM---TRTSSWAALYFIAL 2802
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   TR       Y  AL
Sbjct: 1603 PYYADYSHTRRSIH-SLCTSHYLDLF--------ITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPAL 1840          

HSP 6 Score: 82.0333 bits (201), Expect = 9.177e-15
Identity = 61/173 (35.26%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVMAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 75.0998 bits (183), Expect = 1.308e-12
Identity = 71/224 (31.70%), Postives = 119/224 (53.12%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W ++S K RR    +V SKYF+  I   I +N ++M  E+H  P  L  AL++ N  FTS F LE LLKL+A G   YI + +N  D ++VI+S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  776 WASFSSKLRR----IVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 981          

HSP 8 Score: 70.4774 bits (171), Expect = 3.085e-11
Identity = 65/282 (23.05%), Postives = 138/282 (48.94%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSIL-------RTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N L +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F++EM++K++  G+L     Y+++ +N+ DG++V++ ++++     ++G + +       R  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1291 QNRLRVSCQKV---IAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1569          

HSP 9 Score: 63.929 bits (154), Expect = 2.676e-9
Identity = 33/118 (27.97%), Postives = 64/118 (54.24%), Query Frame = 1
Query:  334 VFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
              ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+ +  +  G
Sbjct: 1284 ALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERP--DIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGG 1399          

HSP 10 Score: 55.8398 bits (133), Expect = 9.279e-7
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 1
Query: 4462 NQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  356 NGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 431          

HSP 11 Score: 54.6842 bits (130), Expect = 1.848e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1497 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1576          

HSP 12 Score: 52.7582 bits (125), Expect = 7.150e-6
Identity = 23/75 (30.67%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R +T+
Sbjct:  953 NFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTD 1027          

HSP 13 Score: 52.373 bits (124), Expect = 8.821e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  360 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQR 437          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1h (calcium channel, voltage-dependent, T type, alpha 1H subunit [Source:MGI Symbol;Acc:MGI:1928842])

HSP 1 Score: 648.662 bits (1672), Expect = 0.000e+0
Identity = 399/646 (61.76%), Postives = 497/646 (76.93%), Query Frame = 1
Query: 3814 AKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE----EAKELAREKQLKDEENNENTLI 5730
            + R   +LY+F   N+ R  CQ ++  K FD++VL FI LNCIT+A+ERP I P S ER FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + + TK +C   +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVG++ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I LNVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   + ED     C+S +  ++P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++ +    +    A+E Q   E +  N L+
Sbjct: 1172 SSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAEC-HAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CTREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQ-GTEPDTPNLLV 1810          

HSP 2 Score: 337.035 bits (863), Expect = 1.189e-92
Identity = 217/396 (54.80%), Postives = 280/396 (70.71%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK 1581
            M +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP     D    +++C+NWNQYY  C+ GE NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K
Sbjct:    1 MLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMME-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQA--EDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRK 382          

HSP 3 Score: 335.88 bits (860), Expect = 2.170e-92
Identity = 190/324 (58.64%), Postives = 234/324 (72.22%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELV 3111
            K++ +V+   F RGI+ AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGI+V++ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V++RT+DNVATF  LL+LFIFIFSILGM LFGCKF    ++  T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   +  +   K       LR +Q     +  L  +      G     S  PP+I HTAATPMPTP+SSP+ ++ 
Sbjct:  676 KLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDK-------LRDVQATEMKMYSLAVTPNGHLEGRG---SLPPPLITHTAATPMPTPKSSPHLDMA 988          

HSP 4 Score: 112.849 bits (281), Expect = 3.586e-24
Identity = 73/278 (26.26%), Postives = 138/278 (49.64%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  673 FSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNAL--EISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIVVIISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------SLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 910          

HSP 5 Score: 82.0333 bits (201), Expect = 9.482e-15
Identity = 67/257 (26.07%), Postives = 126/257 (49.03%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM---TRTSSWAALYFIAL--MTFGNYVL 2826
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   TR       Y  AL  + F  +VL
Sbjct: 1497 PYYADYSHTRRSIH-SLCTSHYLDLF--------ITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPVYFVTFVL 1744          

HSP 6 Score: 75.485 bits (184), Expect = 8.063e-13
Identity = 71/224 (31.70%), Postives = 119/224 (53.12%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W ++S K RR    +V SKYF+  I   I +N ++M  E+H  P  L  AL++ N  FTS F LE LLKL+A G   YI + +N  D ++VI+S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  670 WASFSSKLRR----IVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 875          

HSP 7 Score: 75.0998 bits (183), Expect = 1.129e-12
Identity = 57/156 (36.54%), Postives = 88/156 (56.41%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:    1 MLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVMAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 148          

HSP 8 Score: 70.0922 bits (170), Expect = 3.770e-11
Identity = 66/286 (23.08%), Postives = 139/286 (48.60%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSIL-------RTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +N L +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F++EM++K++  G+L     Y+++ +N+ DG++V++ ++++     ++G + +       R  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1185 QNRLRVSCQKV---IAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1467          

HSP 9 Score: 63.929 bits (154), Expect = 2.639e-9
Identity = 33/118 (27.97%), Postives = 64/118 (54.24%), Query Frame = 1
Query:  334 VFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
              ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+ +  +  G
Sbjct: 1178 ALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERP--DIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGG 1293          

HSP 10 Score: 55.4546 bits (132), Expect = 1.145e-6
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 1
Query: 4462 NQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  250 NGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 325          

HSP 11 Score: 54.299 bits (129), Expect = 2.610e-6
Identity = 26/80 (32.50%), Postives = 48/80 (60.00%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1391 NFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1470          

HSP 12 Score: 52.373 bits (124), Expect = 9.693e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  254 NFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQR 331          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1g (calcium channel, voltage-dependent, T type, alpha 1G subunit [Source:MGI Symbol;Acc:MGI:1201678])

HSP 1 Score: 645.195 bits (1663), Expect = 0.000e+0
Identity = 388/612 (63.40%), Postives = 475/612 (77.61%), Query Frame = 1
Query: 3778 LSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDC-QRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESH 5607
            L  CC        +R   S Y+F   ++FR  C  I+  K FD++VL  I LNCIT+AMERP I PHS ER+FLT++NYIFT +F  EM +KV++ G   G+QAYL+S WN++DG LV IS++D+ ++++     +I G+LRV RLLRTLRPLRVISRA GLKLVV+TL+SSL+PIGNIV+ICC FFIIFGILGVQLFKGKF+ C G   + +  K+DC + S   W   KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAVGV+ QP  NHN W L+YFISFLL+VAFFVLNMFVGVVVENFHKC+++QE+EE  RR  KR +RLEKKR+  +   Y+ +YS++R  ++ + TS Y DL I GVIGLNV+TMA E +  PQ+L  ALK+ NY FT  FVLE++ KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA G+RALLDTV+QALPQVGNLGLLF LLF+IFAALGVELFG LEC + +PC GLGRHA F NFGMAFLTLFR++TGDNWNGIMKDTLR       DC Q + C + +I+PIYFV FVL AQFVLVNVV+AVLMKHLEES+
Sbjct: 1245 LPACC-------RERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASY-RWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLR-------DCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESN 1841          

HSP 2 Score: 369.777 bits (948), Expect = 1.579e-102
Identity = 229/466 (49.14%), Postives = 307/466 (65.88%), Query Frame = 1
Query:  226 DFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHP-GLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIR----HSKTAQGNYQNK 1608
            D +     +     ++  G+  +  E L Y      VF    Q+  PR WCL+ +  PWFE++SM +I +NC+TL M++PC D  C++ +C+I+QAFD  IFAFF  EMV+K++A+G  G   Y GDTWNRLD FIVI G +E       N+  +A+RT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L   E+F  P  ++L  +YQ       N  +  FICS  + NG+  C  +P                 A     N  +  +CVNWNQYYT+C  GE NP++GAINFDNIG+AW+AIFQVI++E WVDIMY+V DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE++ M  +R RF S+  +  S  EP +CY E++K +  I+ K  RR+ ++   + +R     S  A+G  + +
Sbjct:   24 DLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDL-QNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLP--ENFSLPLSVDLEPYYQTE-----NEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAY----NSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQ 477          

HSP 3 Score: 338.191 bits (866), Expect = 7.322e-93
Identity = 219/478 (45.82%), Postives = 289/478 (60.46%), Query Frame = 1
Query: 1744 YPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTR--------DNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAY-------QIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELC-QQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKD----------QTGENQKI--SSQPPVIIHTAATPMPTPRSS 3093
            YP   ++ PP+   + ++++V  SP  P     +I     S+  K         LLE  ST A HS+ +        D+      + P         P+S        +S A        Q   L D + + R     +   +    +  +     + +V+   F RGI+IAIL+NTLSMGIEYH+QP +LT  LE SNIVF+ LF +EM+LKL+  G  GYIKN +N+FDG+IVV+ V E+  QQGG  GLS+LRTFRL+R+LKLVRF+PAL++QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF +  +     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYFIALMTFGNYVLFNLLVAILVEGF +E + +++     + S  +   N++     + +SE     + S+  D            GE+ ++  S  PP+IIHTAATPM  P+SS
Sbjct:  590 YPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHK---------LLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGG--GLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKREDTSG-QLSCIQLPVNSQGGDATKSESEP-DFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSS 1054          

HSP 4 Score: 108.997 bits (271), Expect = 6.915e-23
Identity = 74/275 (26.91%), Postives = 136/275 (49.45%), Query Frame = 1
Query: 3877 QHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            + IVD K F   ++  I +N +++ +E    P    E L   L ++N +FT +F  EM++K+L    + G   Y+K+ +NI DG +V IS+ +     ++G       +LR FRL+R L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF         A +   D L          + NFD+L  A++ +F + +++ W +++Y G+ +           + WA +YFI+ +    + + N+ V ++VE F 
Sbjct:  737 RKIVDSKYFGRGIMIAILVNTLSMGIEYHEQP----EELTNALEISNIVFTSLFALEMLLKLL----VYGPFGYIKNPYNIFDGVIVVISVWE-----IVGQQGGGLSVLRTFRLMRVLKLVRFL---PALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKF---------ASERDGDTLP--------DRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMAST----------SSWAALYFIALMTFGNYVLFNLLVAILVEGFQ 968          

HSP 5 Score: 83.5741 bits (205), Expect = 3.738e-15
Identity = 58/235 (24.68%), Postives = 123/235 (52.34%), Query Frame = 1
Query: 2215 ILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC----TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM--------TRTSSWAALYFIALMTFGNYVLFNLLVAILVE 2856
            I +N ++M +E++QQP  L + L+  N +F+++F++E + KL+  G   + ++ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FIF+ LG+ LFG   C      E   R    +NF     + +T+F++ T ++WN ++ + +           +  + +YF++ +    +VL N+++A+L++
Sbjct: 1604 IGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFG---MAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMK 1835          

HSP 6 Score: 80.4925 bits (197), Expect = 2.958e-14
Identity = 70/215 (32.56%), Postives = 108/215 (50.23%), Query Frame = 1
Query: 3739 EDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +DP   D EA     +  P P       +Y      S D++ R  C   V    F+ + +  I LNC+TL M RP   I   S+    L   +      F  EM++K+++ G+  G + YL   WN +D F+V   +L+  + +     N  F  +R    +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   39 KDPGSADSEA-----EGLPYPALAPVVFFY-----LSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIF-GKKCYLGDTWNRLDFFIVIAGMLEYSLDL----QNVSFSAVRT---VRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 235          

HSP 7 Score: 72.0182 bits (175), Expect = 1.128e-11
Identity = 67/215 (31.16%), Postives = 113/215 (52.56%), Query Frame = 1
Query: 4816 WR---RTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            WR    T   +V SKYF   I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+  G   YI + +N  D +IV++S+  +V  +            + V+R  R+ RVLKL++    ++  L  +++ +  V    +L  L  +IF+ LG+ LFG    S+ +    L    +F++   A +T+F+I T ++WN ++ + +
Sbjct:  729 WRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGG---------LSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDT-LPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGM 933          

HSP 8 Score: 66.2402 bits (160), Expect = 6.596e-10
Identity = 38/116 (32.76%), Postives = 66/116 (56.90%), Query Frame = 1
Query:  340 HIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
            +IF      R  C +IIT   F+ + + II +NCIT++M +P  D +  +++   +   +YI  A F  EM +K++A+G+  G+  Y   +WN LD  +V++  I+IL S  ++ G
Sbjct: 1258 YIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPH--SAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSG 1371          

HSP 9 Score: 64.3142 bits (155), Expect = 2.158e-9
Identity = 63/270 (23.33%), Postives = 123/270 (45.56%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIE-LCQQGGTSGLSILRTFRLLR---------ILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTC---------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            F   +L+ I +N +++ +E  +      +   L  SN +F+ +FL EM +K++  G       Y+++ +N+ DG++V++ VI+ L      SG  IL   R+LR         ++   + +  + + L   ++ I N+        +   I    G+ LF  KF  C  E+     NK  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +    + + N+ V ++VE F   R
Sbjct: 1279 FDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGIL---GVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCR 1545          

HSP 10 Score: 56.6102 bits (135), Expect = 4.374e-7
Identity = 28/95 (29.47%), Postives = 51/95 (53.68%), Query Frame = 1
Query: 1150 TDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            +DC            NFDN+G A +++F + S + WVDIMY   DA          H+ W  +YF++ ++I +FF++N+ + V+   F + ++ +
Sbjct: 1454 SDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQ 1548          

HSP 11 Score: 55.4546 bits (132), Expect = 1.219e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
             +C     N +K    NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  321 TNCSAGEHNPFKG-AINFDNIGYAWIAIFQVITLEGWVDIMYFVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 405          

HSP 12 Score: 53.9138 bits (128), Expect = 3.179e-6
Identity = 23/72 (31.94%), Postives = 43/72 (59.72%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF--SETKRRE 1404
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YF+AL+  G++ + NL + ++   F   E  +RE
Sbjct:  905 NFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEIGKRE 976          

HSP 13 Score: 52.373 bits (124), Expect = 8.714e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  336 NFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQR 413          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Mouse
Match: Cacna1g (calcium channel, voltage-dependent, T type, alpha 1G subunit [Source:MGI Symbol;Acc:MGI:1201678])

HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0
Identity = 388/612 (63.40%), Postives = 475/612 (77.61%), Query Frame = 1
Query: 3778 LSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDC-QRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESH 5607
            L  CC        +R   S Y+F   ++FR  C  I+  K FD++VL  I LNCIT+AMERP I PHS ER+FLT++NYIFT +F  EM +KV++ G   G+QAYL+S WN++DG LV IS++D+ ++++     +I G+LRV RLLRTLRPLRVISRA GLKLVV+TL+SSL+PIGNIV+ICC FFIIFGILGVQLFKGKF+ C G   + +  K+DC + S   W   KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAVGV+ QP  NHN W L+YFISFLL+VAFFVLNMFVGVVVENFHKC+++QE+EE  RR  KR +RLEKKR+  +   Y+ +YS++R  ++ + TS Y DL I GVIGLNV+TMA E +  PQ+L  ALK+ NY FT  FVLE++ KLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA G+RALLDTV+QALPQVGNLGLLF LLF+IFAALGVELFG LEC + +PC GLGRHA F NFGMAFLTLFR++TGDNWNGIMKDTLR       DC Q + C + +I+PIYFV FVL AQFVLVNVV+AVLMKHLEES+
Sbjct: 1222 LPACC-------RERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASY-RWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLR-------DCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESN 1818          

HSP 2 Score: 369.392 bits (947), Expect = 1.838e-102
Identity = 229/466 (49.14%), Postives = 307/466 (65.88%), Query Frame = 1
Query:  226 DFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHP-GLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIR----HSKTAQGNYQNK 1608
            D +     +     ++  G+  +  E L Y      VF    Q+  PR WCL+ +  PWFE++SM +I +NC+TL M++PC D  C++ +C+I+QAFD  IFAFF  EMV+K++A+G  G   Y GDTWNRLD FIVI G +E       N+  +A+RT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L   E+F  P  ++L  +YQ       N  +  FICS  + NG+  C  +P                 A     N  +  +CVNWNQYYT+C  GE NP++GAINFDNIG+AW+AIFQVI++E WVDIMY+V DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE++ M  +R RF S+  +  S  EP +CY E++K +  I+ K  RR+ ++   + +R     S  A+G  + +
Sbjct:   24 DLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIFGKKCYLGDTWNRLDFFIVIAGMLEYSLDL-QNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLP--ENFSLPLSVDLEPYYQTE-----NEDESPFICSQPRENGMRSCRSVPTLRGEGGGGPPCGLDYEAY----NSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQ 477          

HSP 3 Score: 334.339 bits (856), Expect = 1.206e-91
Identity = 216/470 (45.96%), Postives = 280/470 (59.57%), Query Frame = 1
Query: 1744 YPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTR--------DNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAY-------QIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELC-QQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE--RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKI--SSQPPVIIHTAATPMPTPRSS 3093
            YP   ++ PP+   + ++++V  SP  P     +I     S+  K         LLE  ST A HS+ +        D+      + P         P+S        +S A        Q   L D + + R     +   +    +  +     + +V+   F RGI+IAIL+NTLSMGIEYH+QP +LT  LE SNIVF+ LF +EM+LKL+  G  GYIKN +N+FDG+IVV+ V E+  QQGG  GLS+LRTFRL+R+LKLVRF+PAL++QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF +  +     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYFIALMTFGNYVLFNLLVAILVEGF +E      +     +  S+       K+L ++                   GE+ ++  S  PP+IIHTAATPM  P+SS
Sbjct:  590 YPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHK---------LLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGG--GLSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRLALV-----------------ALGEHSELRKSLLPPLIIHTAATPMSLPKSS 1031          

HSP 4 Score: 108.612 bits (270), Expect = 8.160e-23
Identity = 74/275 (26.91%), Postives = 136/275 (49.45%), Query Frame = 1
Query: 3877 QHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            + IVD K F   ++  I +N +++ +E    P    E L   L ++N +FT +F  EM++K+L    + G   Y+K+ +NI DG +V IS+ +     ++G       +LR FRL+R L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF         A +   D L          + NFD+L  A++ +F + +++ W +++Y G+ +           + WA +YFI+ +    + + N+ V ++VE F 
Sbjct:  737 RKIVDSKYFGRGIMIAILVNTLSMGIEYHEQP----EELTNALEISNIVFTSLFALEMLLKLL----VYGPFGYIKNPYNIFDGVIVVISVWE-----IVGQQGGGLSVLRTFRLMRVLKLVRFL---PALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKF---------ASERDGDTLP--------DRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMAST----------SSWAALYFIALMTFGNYVLFNLLVAILVEGFQ 968          

HSP 5 Score: 83.1889 bits (204), Expect = 4.232e-15
Identity = 58/235 (24.68%), Postives = 123/235 (52.34%), Query Frame = 1
Query: 2215 ILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC----TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM--------TRTSSWAALYFIALMTFGNYVLFNLLVAILVE 2856
            I +N ++M +E++QQP  L + L+  N +F+++F++E + KL+  G   + ++ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FIF+ LG+ LFG   C      E   R    +NF     + +T+F++ T ++WN ++ + +           +  + +YF++ +    +VL N+++A+L++
Sbjct: 1581 IGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFG---MAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMK 1812          

HSP 6 Score: 80.4925 bits (197), Expect = 2.831e-14
Identity = 70/215 (32.56%), Postives = 108/215 (50.23%), Query Frame = 1
Query: 3739 EDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +DP   D EA     +  P P       +Y      S D++ R  C   V    F+ + +  I LNC+TL M RP   I   S+    L   +      F  EM++K+++ G+  G + YL   WN +D F+V   +L+  + +     N  F  +R    +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   39 KDPGSADSEA-----EGLPYPALAPVVFFY-----LSQDSRPRSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALGIF-GKKCYLGDTWNRLDFFIVIAGMLEYSLDL----QNVSFSAVRT---VRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 235          

HSP 7 Score: 72.0182 bits (175), Expect = 1.145e-11
Identity = 67/215 (31.16%), Postives = 113/215 (52.56%), Query Frame = 1
Query: 4816 WR---RTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            WR    T   +V SKYF   I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+  G   YI + +N  D +IV++S+  +V  +            + V+R  R+ RVLKL++    ++  L  +++ +  V    +L  L  +IF+ LG+ LFG    S+ +    L    +F++   A +T+F+I T ++WN ++ + +
Sbjct:  729 WRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGG---------LSVLRTFRLMRVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDT-LPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGM 933          

HSP 8 Score: 66.2402 bits (160), Expect = 6.532e-10
Identity = 38/116 (32.76%), Postives = 66/116 (56.90%), Query Frame = 1
Query:  340 HIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
            +IF      R  C +IIT   F+ + + II +NCIT++M +P  D +  +++   +   +YI  A F  EM +K++A+G+  G+  Y   +WN LD  +V++  I+IL S  ++ G
Sbjct: 1235 YIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPH--SAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSG 1348          

HSP 9 Score: 64.3142 bits (155), Expect = 2.229e-9
Identity = 63/270 (23.33%), Postives = 123/270 (45.56%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIE-LCQQGGTSGLSILRTFRLLR---------ILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN-----NKRTC---------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            F   +L+ I +N +++ +E  +      +   L  SN +F+ +FL EM +K++  G       Y+++ +N+ DG++V++ VI+ L      SG  IL   R+LR         ++   + +  + + L   ++ I N+        +   I    G+ LF  KF  C  E+     NK  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +    + + N+ V ++VE F   R
Sbjct: 1256 FDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGIL---GVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCR 1522          

HSP 10 Score: 56.6102 bits (135), Expect = 4.443e-7
Identity = 28/95 (29.47%), Postives = 51/95 (53.68%), Query Frame = 1
Query: 1150 TDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            +DC            NFDN+G A +++F + S + WVDIMY   DA          H+ W  +YF++ ++I +FF++N+ + V+   F + ++ +
Sbjct: 1431 SDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQ 1525          

HSP 11 Score: 55.4546 bits (132), Expect = 1.208e-6
Identity = 29/96 (30.21%), Postives = 53/96 (55.21%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
             +C     N +K    NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  321 TNCSAGEHNPFKG-AINFDNIGYAWIAIFQVITLEGWVDIMYFVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 405          

HSP 12 Score: 52.7582 bits (125), Expect = 6.880e-6
Identity = 22/75 (29.33%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YF+AL+  G++ + NL + ++   F    +  + E+E
Sbjct:  905 NFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESE 979          

HSP 13 Score: 52.373 bits (124), Expect = 9.468e-6
Identity = 23/78 (29.49%), Postives = 45/78 (57.69%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E +L+   R
Sbjct:  336 NFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQR 413          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|O95180|CAC1H_HUMAN (Voltage-dependent T-type calcium channel subunit alpha-1H OS=Homo sapiens OX=9606 GN=CACNA1H PE=1 SV=4)

HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0
Identity = 413/683 (60.47%), Postives = 508/683 (74.38%), Query Frame = 1
Query: 3667 REKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHL-------RAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIA--PIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAR 5685
            RE A E  DDS  E     R++ V +P     +   C S            R   +LY+FS  N+FR  CQ ++  K FD++VL FI LNC+T+A+ERP I P S ER+FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+GP  + + TK  C + +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVGV+ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+         R P Y+ +YS  RR+I+S+ TS Y DL I  +I +NVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LS+ G+ ++E+E S  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   S ED     C+S + A  P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D EL+ E++ ++ +      R
Sbjct: 1236 REDAAELDDDS--EDSCCLRLHKVLEP----YKPQWCRS------------REAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQC-RAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPSPEAQRRP-YYADYSPTRRSIHSLCTSHYLDLFITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CSREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAELDAEIELEMAQGPGSARR 1893          

HSP 2 Score: 372.859 bits (956), Expect = 1.337e-102
Identity = 237/451 (52.55%), Postives = 306/451 (67.85%), Query Frame = 1
Query:  313 YHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK-----TAQGNYQNKLKRQRK 1626
            Y    ATVF    Q   PR WCL+++  PWFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP          +++C+NWNQYY  C+ G+ NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE++ M  +R R  S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K       QG  Q    RQR+
Sbjct:   72 YPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVVAGMMEYS-LDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPGRRELRMPCTLGWEAYTQPQAEGVGA--ARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRARHLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSAVQG--QGPGHRQRR 506          

HSP 3 Score: 337.806 bits (865), Expect = 8.288e-92
Identity = 189/320 (59.06%), Postives = 237/320 (74.06%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPY 3099
            K++ +V+   F RGI++AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF    +   T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   ++ +   K R         + Q+  L + + ++  +   E +  S  PP+I+ TAATPMPTP+SSP+
Sbjct:  785 KLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSDTDEDKTSVHFEEDFHKLR---------ELQTTELKMCSLAVTPNGHLEGRG-SLSPPLIMCTAATPMPTPKSSPF 1093          

HSP 4 Score: 112.464 bits (280), Expect = 4.331e-23
Identity = 75/280 (26.79%), Postives = 138/280 (49.29%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F   ++  I +N +++ +E    P    E L   L ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +     +   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  782 FSGKLRRIVDSKYFSRGIMMAILVNTLSMGVEYHEQP----EELTNALEISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIIVVISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKF----------------SLKTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1019          

HSP 5 Score: 87.0409 bits (214), Expect = 1.943e-15
Identity = 63/241 (26.14%), Postives = 120/241 (49.79%), Query Frame = 1
Query: 2080 SYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS 2772
            S    RRP+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   S
Sbjct: 1591 SPEAQRRPYYADYSPTRRSIH-SLCTSHYLDLF--------ITFIICVNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSLMGITLEEIEMSAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFSNFGMAFLTLFRVSTGDNWNGIMKDTLRECS 1822          

HSP 6 Score: 80.1073 bits (196), Expect = 2.914e-13
Identity = 61/173 (35.26%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVCNPWFEHVSMLVIMLNCVTLGMFRPCEDVECGSERCNILEAFDAFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVVAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 72.7886 bits (177), Expect = 4.518e-11
Identity = 71/224 (31.70%), Postives = 117/224 (52.23%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W  +S K RR    +V SKYF   I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G   YI + +N  D +IV++S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  779 WVTFSGKLRR----IVDSKYFSRGIMMAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIIVVISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLVKTMDNVATFCTLLMLFIFIFSILGMHLFGCKFSLKTDTGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 984          

HSP 8 Score: 68.1662 bits (165), Expect = 9.549e-10
Identity = 61/282 (21.63%), Postives = 131/282 (46.45%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N   +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F+ EM++K++  G+L     Y+++ +NL DG++V++ ++++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1280 QNRFRVSCQKV---ITHKMFDHVVLVFIFLNCVTIALERPDIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIVVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGPDTRNISTKAQCRAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGVDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1558          

HSP 9 Score: 65.4698 bits (158), Expect = 7.873e-9
Identity = 44/172 (25.58%), Postives = 81/172 (47.09%), Query Frame = 1
Query:  178 PQDNRSRRETLIGNKSDFNL-TDKSKADHCQQETDGTP---------INPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            P   R R   ++   SDF L  D  + D  + + D            + P +        A   ++F      R  C K+IT   F+ + +  I +NC+T+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+
Sbjct: 1211 PTKCRDRDGQVVALPSDFFLRIDSHREDAAELDDDSEDSCCLRLHKVLEPYKPQWCRSREAWALYLFSPQNRFRVSCQKVITHKMFDHVVLVFIFLNCVTIALERP--DIDPGSTERVFLSVSNYIFTAIFVAEMMVKVVALGLLSGEHAYLQSSWNLLDGLLVLVSLVDIV 1380          

HSP 10 Score: 56.225 bits (134), Expect = 4.598e-6
Identity = 32/115 (27.83%), Postives = 60/115 (52.17%), Query Frame = 1
Query: 4399 PHLQAV-KTKNDCLKYSQ---------NEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            P  + V   +N C+ ++Q         +   N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  325 PQAEGVGAARNACINWNQYYNVCRSGDSNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 429          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q9EQ60|CAC1H_RAT (Voltage-dependent T-type calcium channel subunit alpha-1H OS=Rattus norvegicus OX=10116 GN=Cacna1h PE=2 SV=2)

HSP 1 Score: 649.047 bits (1673), Expect = 0.000e+0
Identity = 391/614 (63.68%), Postives = 485/614 (78.99%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            +LY+F   N+ R  CQ ++  K FD++VL FI LNCIT+A+ERP I P S ER FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + + TK +C   +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVG++ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+  +   Y+ +YS  RR+I+S+ TS Y DL I  +I LNVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   + ED     C+S +  ++P+YFV F+L+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++ +
Sbjct: 1284 ALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAEC-HAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRKAQRRPYYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CTREDKH---CLSYLPALSPVYFVTFMLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEMAQ 1891          

HSP 2 Score: 376.711 bits (966), Expect = 6.735e-104
Identity = 241/469 (51.39%), Postives = 312/469 (66.52%), Query Frame = 1
Query:  199 RETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK 1581
            RE   G+ S   L  +S    C  +       P   + Y    ATVF    Q   PR WCL+++  PWFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP     D    +++C+NWNQYY  C+ GE NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K
Sbjct:   38 REEQGGSGSGV-LAPESPGTECGADLGADEEQP---VPYPALAATVFFCLGQTTRPRSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMME-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQA--EDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIMGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRK 488          

HSP 3 Score: 340.887 bits (873), Expect = 8.922e-93
Identity = 197/350 (56.29%), Postives = 241/350 (68.86%), Query Frame = 1
Query: 2074 TDSYSKHRR--PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSP 3096
            T  +S  RR  P  K        +  +    K++ +V+   F RGI+ AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGI+VV+ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V++RT+DNVATF  LL+LFIFIFSILGM LFGCKF    ++  T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K          K R     LR  + +  +L +  N   + +       S  PP+I HTAATPMPTP+SSP
Sbjct:  751 TPGHSNERRRTPLRKASQPGGIGHLWASFSGKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVVISVWEIVGQA-NGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTQLEGDFDKLR----DLRATEMKMYSLAVTPNGHLEGRG------SLPPPLITHTAATPMPTPKSSP 1089          

HSP 4 Score: 115.931 bits (289), Expect = 3.503e-24
Identity = 76/280 (27.14%), Postives = 140/280 (50.00%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F+  ++  I +N +++ +E    P    E L   L ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G +N    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  779 FSGKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQP----EELTNALEISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIVVVISVWE-----IVGQANGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------SLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1016          

HSP 5 Score: 83.1889 bits (204), Expect = 3.304e-14
Identity = 68/261 (26.05%), Postives = 127/261 (48.66%), Query Frame = 1
Query: 2101 PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM---TRTSSWAALYFIALMTFGNYVLFNLLVA 2844
            P+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   TR       Y  AL     Y +  +LVA
Sbjct: 1603 PYYADYSHTRRSIH-SLCTSHYLDLF--------ITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPALSPV--YFVTFMLVA 1852          

HSP 6 Score: 82.4185 bits (202), Expect = 5.472e-14
Identity = 61/173 (35.26%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVMAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 74.7146 bits (182), Expect = 1.101e-11
Identity = 71/224 (31.70%), Postives = 121/224 (54.02%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W ++S K RR    +V SKYF+  I   I +N ++M  E+H  P+ L  AL++ N  FTS F LE LLKL+A G   YI + +N  D ++V++S+  +V    ++N        + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  776 WASFSGKLRR----IVDSKYFNRGIMAAILVNTLSMGVEYHEQPEELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVVISVWEIV---GQAN------GGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 981          

HSP 8 Score: 70.4774 bits (171), Expect = 2.289e-10
Identity = 65/282 (23.05%), Postives = 138/282 (48.94%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSIL-------RTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N L +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F++EM++K++  G+L     Y+++ +N+ DG++V++ ++++     ++G + +       R  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1291 QNRLRVSCQKV---IAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAMASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1569          

HSP 9 Score: 63.929 bits (154), Expect = 2.348e-8
Identity = 33/118 (27.97%), Postives = 64/118 (54.24%), Query Frame = 1
Query:  334 VFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
              ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+ +  +  G
Sbjct: 1284 ALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERP--DIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAMASAGG 1399          

HSP 10 Score: 55.8398 bits (133), Expect = 5.261e-6
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 1
Query: 4462 NQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  356 NGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIMGSFFMINLCLVVIATQFSETKQRENQ 431          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|O88427|CAC1H_MOUSE (Voltage-dependent T-type calcium channel subunit alpha-1H OS=Mus musculus OX=10090 GN=Cacna1h PE=1 SV=3)

HSP 1 Score: 647.121 bits (1668), Expect = 0.000e+0
Identity = 401/653 (61.41%), Postives = 498/653 (76.26%), Query Frame = 1
Query: 3814 AKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHL-------RAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE----EAKELAREKQLKDEENNENTLI 5730
            + R   +LY+F   N+ R  CQ ++  K FD++VL FI LNCIT+A+ERP I P S ER FL+V+NYIFT IF  EM++KV++ GLL G+ AYL+S WN++DG LV +SL+D+ + +      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSLRPIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + + TK +C   +   W  +KYNFDNLGQALM+LFVLSSKDGWV IMY G+DAVG++ QP +NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLE++R+         R P Y+ +YS  RR+I+S+ TS Y DL I  +I LNVITM+ E +  P+ L  ALK  NY FT  FV EA LKLVA G  R+  D+WNQLD+ IV+LSI G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFGRLECS+ NPC GL RHA F NFGMAFLTLFR++TGDNWNGIMKDTLRE   + ED     C+S +  ++P+YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E++ ++ +    +    A+E Q   E +  N L+
Sbjct: 1278 SSRESWALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAEC-HAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLERRRRSTFPNPEAQRRP-YYADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRE--CTREDKH---CLSYLPALSPVYFVTFVLVAQFVLVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPSTAQESQ-GTEPDTPNLLV 1922          

HSP 2 Score: 374.4 bits (960), Expect = 3.674e-103
Identity = 240/469 (51.17%), Postives = 312/469 (66.52%), Query Frame = 1
Query:  199 RETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYL------NLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--QICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSK 1581
            RE   G+ S   L  +S    C  +       P   + Y    ATVF    Q   PR WCL++++  WFE +SM +I +NC+TL M++PC D  C + +C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L      N N +F  P      +YQ          +  FICS+ ++NG+ KCS IP    L    +L  +   QP     D    +++C+NWNQYY  C+ GE NP+ GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE + M  +R R+ S++ +  S  EP +CY E++K +  I  K+ RR ++LY + Q R  K
Sbjct:   38 REEQRGSGSSV-LAPESPGTECGADLGADEEQP---VPYPALAATVFFCLGQTTRPRSWCLRLVSRRWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLFGQKCYLGDTWNRLDFFIVMAGMME-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRP------YYQTE-----EGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQA--EDGGAGRNACINWNQYYNVCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQLMREQRARYLSNDSTLASFSEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRK 488          

HSP 3 Score: 338.191 bits (866), Expect = 6.480e-92
Identity = 190/324 (58.64%), Postives = 234/324 (72.22%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELV 3111
            K++ +V+   F RGI+ AIL+NTLSMG+EYH+QP +LT  LE SNIVF+ +F +EM+LKL+  G LGYI+N +N+FDGI+V++ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V++RT+DNVATF  LL+LFIFIFSILGM LFGCKF    ++  T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TSSWAALYF+ALMTFGNYVLFNLLVAILVEGF +E        DE K   +  +   K       LR +Q     +  L  +      G     S  PP+I HTAATPMPTP+SSP+ ++ 
Sbjct:  782 KLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIVGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDK-------LRDVQATEMKMYSLAVTPNGHLEGRG---SLPPPLITHTAATPMPTPKSSPHLDMA 1094          

HSP 4 Score: 114.39 bits (285), Expect = 9.070e-24
Identity = 73/278 (26.26%), Postives = 138/278 (49.64%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            F  K + IVD K F+  ++  I +N +++ +E    P      L   ++N +FT +F  EM++K+L+ G L     Y+++ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  779 FSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNAL--EISNIVFTSMFALEMLLKLLACGPL----GYIRNPYNIFDGIVVIISVWE-----IVGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------SLKTDSGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SSWAALYFVALMTFGNYVLFNLLVAILVEGFQ 1016          

HSP 5 Score: 87.4261 bits (215), Expect = 1.449e-15
Identity = 66/250 (26.40%), Postives = 123/250 (49.20%), Query Frame = 1
Query: 2092 HRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM---TRTSSWAALYFIAL 2802
             RRP+  + + T+R  + S+C      L         I   I +N ++M +E++ QP  L + L++ N VF+I+F+ E  LKL+  G   + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ +N      R   F +   + +T+F++ T ++WN ++ + +   TR       Y  AL
Sbjct: 1606 QRRPYYADYSHTRRSIH-SLCTSHYLDLF--------ITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAALKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFGMAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYLPAL 1846          

HSP 6 Score: 85.8853 bits (211), Expect = 5.070e-15
Identity = 61/173 (35.26%), Postives = 98/173 (56.65%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V ++ F+++ +  I LNC+TL M RP   +   S+    L   +      F  EM+IK+++ GL  G + YL   WN +D F+V   +++  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   90 RSWCLRLVSRRWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIFAFFAVEMVIKMVALGLF-GQKCYLGDTWNRLDFFIVMAGMMEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 254          

HSP 7 Score: 74.7146 bits (182), Expect = 1.030e-11
Identity = 71/224 (31.70%), Postives = 119/224 (53.12%), Query Frame = 1
Query: 4798 WENYS-KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W ++S K RR    +V SKYF+  I   I +N ++M  E+H  P  L  AL++ N  FTS F LE LLKL+A G   YI + +N  D ++VI+S+  +V  + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  776 WASFSSKLRR----IVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALEISNIVFTSMFALEMLLKLLACGPLGYIRNPYNIFDGIVVIISVWEIV-GQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 981          

HSP 8 Score: 70.4774 bits (171), Expect = 2.123e-10
Identity = 65/282 (23.05%), Postives = 138/282 (48.94%), Query Frame = 1
Query: 2134 KNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSIL-------RTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTCD---------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +N L +  QK+   +    F   +L+ I +N +++ +E        T+   L  SN +F+ +F++EM++K++  G+L     Y+++ +N+ DG++V++ ++++     ++G + +       R  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF   E         K  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F
Sbjct: 1291 QNRLRVSCQKV---IAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGTDTRNITTKAECHAAHYRWVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENF 1569          

HSP 9 Score: 64.3142 bits (155), Expect = 1.795e-8
Identity = 33/118 (27.97%), Postives = 64/118 (54.24%), Query Frame = 1
Query:  334 VFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
              ++F      R  C K+I    F+ + +  I +NCIT+++ +P  D +  +++   +   +YI  A F  EM++K++A+G L G+  Y   +WN LD  +V+V  ++I+ +  +  G
Sbjct: 1284 ALYLFPPQNRLRVSCQKVIAHKMFDHVVLVFIFLNCITIALERP--DIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLWGEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGG 1399          

HSP 10 Score: 55.8398 bits (133), Expect = 6.173e-6
Identity = 31/86 (36.05%), Postives = 50/86 (58.14%), Query Frame = 1
Query: 4462 NQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH--KCQENQ 4713
            N   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F   K +ENQ
Sbjct:  356 NGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENQ 431          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q9Z0Y8|CAC1I_RAT (Voltage-dependent T-type calcium channel subunit alpha-1I OS=Rattus norvegicus OX=10116 GN=Cacna1i PE=1 SV=3)

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 389/611 (63.67%), Postives = 487/611 (79.71%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDL 5658
            S+Y+FS +N+FR  CQ I+  K FDY+VL FI LNCIT+A+ERP I   S ER+FLTV+NYIFT IF GEM +KV+S GL  G+QAYL+S WN++DGFLVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSL+PIGNIVLICC FFIIFGILGVQLFKGKFY+C G   + +  ++DC+  +   W + KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAV V+ QP  NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLEKKR+  +   Y+  Y   R  I+S+ TS Y D+ I  +I LNV+TM+ E +  P  L+TALK  NY FT+ FVLEA+LKLVA GL R+  D+WNQLD+ IV+LS+ G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C+D NPC G+ RHA FENFGMAFLTLF+++TGDNWNGIMKDTLR+ C   E   R+C  S   ++P+YFV FVL AQFVL+NVVVAVLMKHL++S+K   +D E++ E++ ++
Sbjct: 1108 SVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVA-ANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRD-CTHDE---RSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEM 1713          

HSP 2 Score: 360.918 bits (925), Expect = 5.630e-99
Identity = 235/481 (48.86%), Postives = 304/481 (63.20%), Query Frame = 1
Query:  298 NESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQIC--KQPARPFT---DNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGN----YQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISG 1713
            N  + +       F    Q   PR+WC+K++  PWFE +SM +I +NC+TL MYQPC D  C + +C+I+Q FD  IF FF  EMV+K++A+G  G   Y GDTWNRLD FIV+ G +E       NI L+AIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRC+L  N + +   + L  +YQ          +  FICS   +NGI+ C +IPP    L     + C  K     F     +L     CVNWN+YY  C+ G  NP++GAINFDNIG+AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE   M  +R+R+ SS+ +  S  EP  CY EI + +  I+ K  RR + LY  +Q R      G           ++  HC +C       P+D   H  ++V  IS 
Sbjct:   44 NPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDL-QNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQG-DVALPPYYQPEED-----DEMPFICSLTGDNGIMGCHEIPP----LKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSS-TVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQAMGPGTPAPAKPGPHAKEPSHCKLC---PRHSPLDPTPH--TLVQPISA 507          

HSP 3 Score: 273.092 bits (697), Expect = 4.647e-72
Identity = 169/317 (53.31%), Postives = 223/317 (70.35%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER------LDEQKLLHNYRQ--SLKKRRANNKQLRIMQ-------HQSENLVLLTNSIQKDQTGENQKISSQP-PVII 3054
            ++K++ +V+   F RGI++AIL+NT+SMGIE+H+QP +LT  LE  N+VF+ +F +EMILKL   G+  Y++N +N+FD IIV++ + E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM +FGCKF    +   T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  T+ WA+LYF+ALMTFGNYVLFNLLVAILVEGF +E        DE +   N  +   L +   N++ L++         H   +L L  +       G   ++S QP PV++
Sbjct:  588 REKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQA-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEEFDKLPEGLDNSRDLKLCPIPMTPNGHLDPSLPLGAHLGPAGTMGTAPRLSLQPDPVLV 903          

HSP 4 Score: 95.9005 bits (237), Expect = 3.895e-18
Identity = 64/250 (25.60%), Postives = 128/250 (51.20%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS-----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQK 2889
            I   I +N ++M +E++ QP  L   L++ N +F+ +F++E +LKL+  G+  + K+ +N  D  IV+L V+ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+FQ+ T ++WN ++ + +   +             + LYF++ +    +VL N++VA+L++       + Q+
Sbjct: 1452 ITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQE 1701          

HSP 5 Score: 93.9745 bits (232), Expect = 1.560e-17
Identity = 70/279 (25.09%), Postives = 134/279 (48.03%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            R+K + IVD K F+  ++  I +N +++ +E    P    E L   L + N +FT +F  EMI+K+ + GL      YL++ +NI         ++ + I  ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ +F  KF                 L+    +    + NFD+L  A++ +F + +++ W  ++Y G+ +             WA +YF++ +    + + N+ V ++VE F 
Sbjct:  588 REKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQP----EELTNILEICNVVFTSMFALEMILKLAAFGLF----DYLRNPYNIF-----DSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKF----------------SLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------TPWASLYFVALMTFGNYVLFNLLVAILVEGFQ 824          

HSP 6 Score: 73.559 bits (179), Expect = 2.632e-11
Identity = 62/275 (22.55%), Postives = 127/275 (46.18%), Query Frame = 1
Query: 2167 KTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM----------TRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +T++    F   +L  I +N +++ +E  Q     T+   L  SN +F+ +F+ EM LK++  G+      Y+++ +N+ DG +V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C   N +    + NFD+L  +++++F + +++ W  ++YNG+          T  + W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1123 QTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1397          

HSP 7 Score: 72.0182 bits (175), Expect = 6.865e-11
Identity = 59/173 (34.10%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+ + +  I LNC+TL M +P   +   S     L V +    I F  EM++K+++ G+  G + YL   WN +D F+V   +++   ++ L + N     L   R +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   67 RNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIF-GKKCYLGDTWNRLDFFIVMAGMVEY--SLDLQNIN-----LSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 231          

HSP 8 Score: 58.9214 bits (141), Expect = 7.366e-7
Identity = 68/220 (30.91%), Postives = 115/220 (52.27%), Query Frame = 1
Query: 4816 WRRT---INSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            WR T   +  +V SKYF+  I   I +N ++M  E H  P+ L   L++ N  FTS F LE +LKL A GL  Y+ + +N  D +IVI+SI   ++ + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ +FG     R +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  584 WRETREKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIW-EIVGQADGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 789          

HSP 9 Score: 56.9954 bits (136), Expect = 2.389e-6
Identity = 33/116 (28.45%), Postives = 60/116 (51.72%), Query Frame = 1
Query:  340 HIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
            ++F      R  C  II    F+ + +  I +NCIT+++ +P       +++   +   +YI  A F  EM +K++++G + G+  Y   +WN LD F+V V  I+I+ S  +  G
Sbjct: 1110 YLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERP--QIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGG 1223          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. UniProt/SwissProt
Match: sp|Q9P0X4|CAC1I_HUMAN (Voltage-dependent T-type calcium channel subunit alpha-1I OS=Homo sapiens OX=9606 GN=CACNA1I PE=1 SV=1)

HSP 1 Score: 637.106 bits (1642), Expect = 0.000e+0
Identity = 389/611 (63.67%), Postives = 487/611 (79.71%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS-AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDL 5658
            S+Y+FS +N+FR  CQ I+  K FDY+VL FI LNCIT+A+ERP I   S ER+FLTV+NYIFT IF GEM +KV+S GL  G+QAYL+S WN++DGFLVF+S++D+ +++      +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL+SSL+PIGNIVLICC FFIIFGILGVQLFKGKFY+C G   + +  ++DC+  +   W + KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAV V+ QP  NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RRLEKKR+  +   Y+  Y   R  I+S+ TS Y D+ I  +I LNV+TM+ E +  P  L+TALK  NY FT+ FVLEA+LKLVA GL R+  D+WNQLD+ IV+LS+ G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L C+D NPC G+ RHA FENFGMAFLTLF+++TGDNWNGIMKDTLR+ C   E   R+C  S   ++P+YFV FVL AQFVL+NVVVAVLMKHL++S+K   +D E++ E++ ++
Sbjct: 1149 SVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMA-ANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCHTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRD-CTHDE---RSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELEM 1754          

HSP 2 Score: 357.451 bits (916), Expect = 5.233e-98
Identity = 239/494 (48.38%), Postives = 311/494 (62.96%), Query Frame = 1
Query:  286 PINPNESLIYHGFYATV-FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQIC--KQPARPFT---DNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIR----------------HSKTAQGNYQNKLKRQ--------RKHCNVCLNSRSQFPIDDH 1677
            P++  +  + H   A + F    Q   PR+WC+K++  PWFE +SM +I +NC+TL MYQPC D +C + +C+I+Q FD  IF FF  EMV+K++A+G  G   Y GDTWNRLD FIV+ G +E       NI L+AIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRC+L  N + +   + L  +YQ          +  FICS   +NGI+ C +IPP    L     + C  K     F     +L     CVNWN+YY  C+ G  NP++GAINFDNIG+AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE   M  +R+R+ SS+ +  S  EP  CY EI + +  I+ K  RR + LY  +Q R                H+K  + +Y  K K Q         +HC       S  P +DH
Sbjct:   41 PLDGADPHVPHPDLAPIAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDL-QNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQG-DVALPPYYQPEED-----DEMPFICSLSGDNGIMGCHEIPP----LKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSS-TVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQSRRQALGPEAPAPAKPGPHAKEPR-HYHGKTKGQGDEGRHLGSRHCQTLHGPAS--PGNDH 519          

HSP 3 Score: 272.707 bits (696), Expect = 6.124e-72
Identity = 155/240 (64.58%), Postives = 194/240 (80.83%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE 2871
            + K++ +V+   F RGI++AIL+NT+SMGIE+H+QP +LT  LE  N+VF+ +F +EMILKL   G+  Y++N +N+FD IIV++ + E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM +FGCKF    +   T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS WA+LYF+ALMTFGNYVLFNLLVAILVEGF +E
Sbjct:  630 RAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQA-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAE 868          

HSP 4 Score: 96.2857 bits (238), Expect = 3.272e-18
Identity = 64/250 (25.60%), Postives = 128/250 (51.20%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS-----------WAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQK 2889
            I   I +N ++M +E++ QP  L   L++ N +F+ +F++E +LKL+  G+  + K+ +N  D  IV+L V+ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  EN      R   F++   + +T+FQ+ T ++WN ++ + +   +             + LYF++ +    +VL N++VA+L++       + Q+
Sbjct: 1493 ITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQE 1742          

HSP 5 Score: 93.5893 bits (231), Expect = 2.322e-17
Identity = 70/279 (25.09%), Postives = 134/279 (48.03%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            R K + IVD K F+  ++  I +N +++ +E    P    E L   L + N +FT +F  EMI+K+ + GL      YL++ +NI         ++ + I  ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ +F  KF                 L+    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  630 RAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQP----EELTNILEICNVVFTSMFALEMILKLAAFGLF----DYLRNPYNIF-----DSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKF----------------SLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SPWASLYFVALMTFGNYVLFNLLVAILVEGFQ 866          

HSP 6 Score: 73.1738 bits (178), Expect = 2.892e-11
Identity = 62/275 (22.55%), Postives = 127/275 (46.18%), Query Frame = 1
Query: 2167 KTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKL-------VRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGM----------TRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            +T++    F   +L  I +N +++ +E  Q     T+   L  SN +F+ +F+ EM LK++  G+      Y+++ +N+ DG +V + +I++     ++G + +     +  L         +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C   N +    + NFD+L  +++++F + +++ W  ++YNG+          T  + W  LYFI+ +   ++ + N+ V ++VE F   R
Sbjct: 1164 QTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCR 1438          

HSP 7 Score: 72.0182 bits (175), Expect = 6.537e-11
Identity = 59/173 (34.10%), Postives = 96/173 (55.49%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C  +V    F+ + +  I LNC+TL M +P   +   S     L V +    I F  EM++K+++ G+  G + YL   WN +D F+V   +++   ++ L + N     L   R +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   69 RNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIF-GKKCYLGDTWNRLDFFIVMAGMVEY--SLDLQNIN-----LSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 233          

HSP 8 Score: 59.3066 bits (142), Expect = 5.095e-7
Identity = 69/226 (30.53%), Postives = 116/226 (51.33%), Query Frame = 1
Query: 4798 WENYSKWRRT---INSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            W     WR T   +  +V SKYF+  I   I +N ++M  E H  P+ L   L++ N  FTS F LE +LKL A GL  Y+ + +N  D +IVI+SI   ++ + +          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ +FG     R +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  620 WLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIW-EIVGQADGG--------LSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 831          

HSP 9 Score: 56.9954 bits (136), Expect = 2.602e-6
Identity = 33/116 (28.45%), Postives = 60/116 (51.72%), Query Frame = 1
Query:  340 HIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
            ++F      R  C  II    F+ + +  I +NCIT+++ +P       +++   +   +YI  A F  EM +K++++G + G+  Y   +WN LD F+V V  I+I+ S  +  G
Sbjct: 1151 YLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERP--QIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLASAGG 1264          

HSP 10 Score: 55.4546 bits (132), Expect = 6.898e-6
Identity = 28/95 (29.47%), Postives = 51/95 (53.68%), Query Frame = 1
Query: 1150 TDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            +DC            NFDN+G A +++F + S + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1347 SDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1441          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. TrEMBL
Match: G0YP35 (Voltage operated calcium channel Cav3 (Fragment) OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 1668.67 bits (4320), Expect = 0.000e+0
Identity = 916/1096 (83.58%), Postives = 1001/1096 (91.33%), Query Frame = 1
Query: 2905 RQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVXXXXXXXXXXXXXXXQSILSINKSFETTSSRNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTN---RSNFIIP--SSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFXXXXXXXXXXXXXQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINKSQQSTTELRLSEKRKLFAKSKINE 6177
            RQSLKKR+AN KQ R+MQ+ ++NL+ L+N+ QKD    +Q+IS+QPP+IIHTAATPMPTPRSSPYNE VTT       +N+   QSILSINKSFETTSSRNINTT + ++   +P + N K N+D++   E  I V K   SNGLNVKLVSIPIPY  N   +SNFIIP  SSILLRQGSINSFMGS+NS++S+I+ DEKKS+SE DEKS+SRQSSFKSFMAYKNKINQLTIP +NRYSLSLDS+GKSNYQFKM YLL REKALEEPDD+NSESQNSSR+NNVED D+ED+EAM+CLSKCCPQPKGIFAKRYYHS+Y+FSVDNQFRQKCQ+ VDKK FDYLVLFFITLNCITLAMERPSIPP SKERLFL+VTNYIFTIIFTGEMI+KVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITI LGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHL  V TKNDC+ + +NEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAV V++QPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTIN+VVTSKYFDLAIAGVIGLNVITMATEFHMMP VLKTALKVFNYFFT+TFVLEA+LKLVALGL RYISDKWNQLDM+IVILSIAGVV+DEVE+NFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECS++NPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLR+KC SSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKE AREKQLK+EENNEN LI+IE DKNNN ++KKEN S+ + Q+KKLSC DC+SIN +L+N+SCQNVHL+NI SKNLS+SHPHLFVN DS+SFT N +T N+NK +QRRLSMKK+  NN+++M NNNK ++  EN    K KIN +QQS+ EL L+EKRKLF ++KI+E
Sbjct:    1 RQSLKKRQANLKQNRLMQNHTDNLLFLSNANQKDIN--DQRISTQPPLIIHTAATPMPTPRSSPYNEFVTTTIPITNQSNS---QSILSINKSFETTSSRNINTTTISDNPQTIPNISNKKTNIDNSAVFEPQIHVPKNIISNGLNVKLVSIPIPYNNNNNNKSNFIIPPRSSILLRQGSINSFMGSINSEKSRII-DEKKSVSELDEKSISRQSSFKSFMAYKNKINQLTIPCTNRYSLSLDSDGKSNYQFKMGYLLTREKALEEPDDTNSESQNSSRINNVEDQDDEDIEAMVCLSKCCPQPKGIFAKRYYHSMYIFSVDNQFRQKCQYFVDKKWFDYLVLFFITLNCITLAMERPSIPPQSKERLFLSVTNYIFTIIFTGEMIVKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITISLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLLNVTTKNDCIAHERNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVSVDVQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINAVVTSKYFDLAIAGVIGLNVITMATEFHMMPHVLKTALKVFNYFFTTTFVLEAILKLVALGLTRYISDKWNQLDMIIVILSIAGVVVDEVENNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSELNPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLRDKCTSSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKEQAREKQLKEEENNENLLINIEKDKNNNCVEKKENESVKKPQMKKLSCDDCYSINEKLKNISCQNVHLINICSKNLSKSHPHLFVNFDSSSFTRNIQTNNINKFIQRRLSMKKDRLNNDTLMLNNNKQIRNIENQWHQKCKINNNQQSSMELTLAEKRKLFTRNKIHE 1090          

HSP 2 Score: 81.6481 bits (200), Expect = 2.166e-11
Identity = 84/314 (26.75%), Postives = 153/314 (48.73%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTR--TSS------------WAALYFIALMTFGNYVLFNLLVAILVEGFSSER---LDEQKLLHNYRQSL---KKRRANNKQLRIMQHQSENLVLLTNSIQKDQ 3009
            ++ I  +V    F   I   I +N ++M  E+H  P  L   L+  N  F+  F++E ILKL+  G+  YI + +N  D IIV+L +  +              + + ++R  R+ R+LKL++    +R  L  +I+ +  V     L  L  +IF+ LG+ LFG   C+  N      R  +F++   + +T+F+I T ++WN ++ + +    TSS             A +YF+  +    +VL N++VA+L++          D+ +L    ++ L    K +A  KQL+  +  +ENL++   +I+KD+
Sbjct:  638 RRTINAVVTSKYFDLAIAGVIGLNVITMATEFHMMPHVLKTALKVFNYFFTTTFVLEAILKLVALGLTRYISDKWNQLDMIIVILSIAGVVVDEVENNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSELNPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLRDKCTSSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKEQAREKQLK-EEENNENLLI---NIEKDK 947          

HSP 3 Score: 79.337 bits (194), Expect = 9.670e-11
Identity = 75/283 (26.50%), Postives = 134/283 (47.35%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFS--NIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIEL--CQQGGTSGLSILRTFRLLRILK---------LVRFMPALR---QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTI-ENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +QK +  V+   F   +L  I +N +++ +E    P Q  + L  S  N +F+I+F  EMI+K++ +G+L     Y+K+G+N+ DG +V + +++L      G S     R F +LR+ +         ++   P L+   Q L   +R I N+     +   F  IF ILG+ LF  KF                C   E N+    + NFD+L  +++ +F + +++ W  ++Y G+   S           WA +YFI+ +    + + N+ V ++VE F
Sbjct:  320 RQKCQYFVDKKWFDYLVLFFITLNCITLAMERPSIPPQSKERLFLSVTNYIFTIIFTGEMIVKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITISLGHSN----RIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIV---LICCTFFIIFGILGVQLFKGKFYYCDGPHLLNVTTKNDCIAHERNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVSVDVQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 595          

HSP 4 Score: 65.0846 bits (157), Expect = 2.531e-6
Identity = 35/123 (28.46%), Postives = 60/123 (48.78%), Query Frame = 1
Query:  295 PNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            P    I+   Y    +IF  +   R  C   +   WF+ L +  I +NCITL+M +P      ++ +   +   +YI    FT EM++K+++ G L GD  Y    WN +D F+V +  +++ 
Sbjct:  296 PQPKGIFAKRYYHSMYIFSVDNQFRQKCQYFVDKKWFDYLVLFFITLNCITLAMERPSIPP--QSKERLFLSVTNYIFTIIFTGEMIVKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLF 416          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. TrEMBL
Match: X1WBF7 (Voltage-dependent T-type calcium channel subunit alpha OS=Danio rerio OX=7955 GN=cacna1ha PE=3 SV=1)

HSP 1 Score: 1286.94 bits (3329), Expect = 0.000e+0
Identity = 874/1848 (47.29%), Postives = 1160/1848 (62.77%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPP---SIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKT------------------AQGNYQNKL-----KRQRKHCNVCLNSRSQFPI-----DDHCHLYSI--------------VNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVL-------NSPRHPNHLISDISSKSVSTETKRKSQYN---------------------------------SQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVXXXXXXXXXXXXXXXQSILSINKSFETTSSRNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXH-LRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAI--IAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELA 5682
            M +I +NC+TL MYQPC D  C++  C ++QAFD  IFAFF  EMVIK+IA+G  G  GY GDTWNRLD FIV+ G +E     G+N  L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC++      ++  L+  S Y  N     +  D  FICS+ K+NG+ +CS +PP    +    N S           T N    +SCVNWNQYY++C+PG+ NP++GA+NFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QF+ETK+RE   M  +R R+ S++ +  S  EP +CY E++K ++ +  K+ RR+ ++YN  Q +  K                     G++   +     + QR+HC+  L++ S  P+      +   + SI               + +S   Q  ++I  Y  +F   P + +P    +  L       N P     LI + S      + + KS  N                                 +Q+LLE+ S P         Y      +  L   D  YP+   S    +   +Y    +T   + H+    + QN+  +  +    ++++  +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SNIVF+ +F++EM+ KL+  GI GYI+N +N+FDG+IVV+ V E+       GLS+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIF FSILGM LFGCKF   +EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS WAALYF+ALMTFGNYVLFNLLVAILVEGF +E  D  K         +++ + N + ++ Q QS      + +   D  G     +  PP+I+ TAATPMPTP+SS                        L     F+ T SR  +   +  +A     + + +++           P    P  +G N         + + RS++    + L R  S+       + +R  +L  E +   E D+    + ++           + L IP   +    LD  G+S       ++LA   A    +DS +               EED++   C  L +     K  + K +   SLY+FS  N+FR  CQ ++  K FDY+VL FI LNCIT+A+ERP I     ERLFL V+NY+FT+IF  EM +KV++ G   G+Q+YLKS WN++DG LVF+SL+D+ ++ L    NRIFGILRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICC FFI+FGILGVQLFKGKF++C+G   + +  K+DCL+ +  +W  +KYNFDNLGQALM+LFVLS KDGWV IMY G+DAVGV+ QP+ NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+ E      KR + +EKKR+  LR P Y+ +YS  R  I+++ T+ Y DL I G+I +NV+TM+ E    P  L  ALK  NY FT  F++EALLKLVA G+ R+  D+WNQLD+ IV+LSI G+ ++E++ N  +PINPTI+R+MRVLRIARVLKLLKMA+G+R+LLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+LEC++ NPC GL  HA FENFGMAFLTLFR++TGDNWNGIMKDTLRE C+ SE      C+S +  ++PIYFV FVLMAQFVLVNVVVAVLMKHLEES+K   +D E++  ++  + +E + L+
Sbjct:    1 MLVILLNCVTLGMYQPCEDLKCQSEWCIVLQAFDDCIFAFFAVEMVIKMIALGIFGINGYLGDTWNRLDFFIVMAGMME-YSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMPSKVRDQY-NLSYMSLYYENE----DGEDNPFICSSSKDNGMKRCSAVPPLKEGVECTLNASFLGHVYSGLSGTGN----YSCVNWNQYYSECKPGDLNPHKGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFAETKQRENMLMKEQRARYRSNDSTLASYSEPGSCYEEMLKYVSHLYRKVKRRLSRIYNSWQSKRRKKVNPNTGAGGGANGHSRHRGYGHWVQSIHSLIQQHQRQHCH--LSNGSPSPVATTGSSEALEMRSITAGQQLTVSSQPTATHNLSSSTQSVHSI--YQGDFREAPQERTPTNVAVPALSRLNGGMNYPTILPSLICNYSGGGSLGKERGKSDANTGHEVIQDFDKLHQQIEAHSHSRVLLQMAGVVPTQLLLEVMSCP---------YCVRALESMDLEIGDSDYPR---SEGDMVYEFSYDAGFITGRSAHHK--MQEKQNRLVQ--FWEDFRERLTRIVDSKYFNRGIMIAILINTLSMGIEYHEQPEELTNILEISNIVFTSMFVLEMLFKLLAFGIFGYIRNPYNIFDGVIVVISVWEIIGH-ADGGLSVLRTFRLLRVLKLVRFLPALRRQLLVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFSLKMENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEG-DANK-----SDGDEEKTSVNSEEKMEQLQS------SANGHVDPCG-----TLPPPIIMRTAATPMPTPKSS------------------------LGPESVFDLTESRRGSAVSIDPNAFDQKSLSSPRRS-----------PCH--PRGSGSN---------WGSRRSSW----NSLGRAPSLKR--KDTSGERESLLSGEGRGSFEDDDIEAEKLTNISG--GSLQHPSSLDIPELPQMGEYLDCNGRS------QHILADLSASLNKEDSIA---------------EEDLDDSFCFRLRRTLAAYKPKWCKDHEEWSLYLFSPHNKFRMMCQKLISHKMFDYVVLVFIFLNCITIALERPHI--QQSERLFLLVSNYVFTVIFVAEMTVKVVALGFYSGNQSYLKSTWNVLDGVLVFVSLIDILVS-LAWTGNRIFGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFFHCEGGDTRNITNKSDCLQ-ANLKWIRRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPERNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEVEARLLELKRQKLMEKKRRKALRRP-YYADYSPARLYIHTLCTNHYLDLFITGIICINVVTMSIEHFNQPSYLDEALKYCNYVFTIIFIIEALLKLVAFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNAALPINPTIIRIMRVLRIARVLKLLKMATGMRSLLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECTENNPCEGLSPHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE-CLPSE----TQCLSYLPWVSPIYFVTFVLMAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAAIKLAILKETRRLS 1715          

HSP 2 Score: 63.5438 bits (153), Expect = 7.943e-6
Identity = 38/119 (31.93%), Postives = 64/119 (53.78%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPS---TGNNI 681
             ++F  +   R  C K+I+   F+ + +  I +NCIT+++ +P    + + S+   +   +Y+    F  EM +K++A+GF  G+  Y   TWN LD  +V V  I+IL S   TGN I
Sbjct: 1106 LYLFSPHNKFRMMCQKLISHKMFDYVVLVFIFLNCITIALERP----HIQQSERLFLLVSNYVFTVIFVAEMTVKVVALGFYSGNQSYLKSTWNVLDGVLVFVSLIDILVSLAWTGNRI 1220          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. TrEMBL
Match: A0A2C9KE73 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106079304 PE=3 SV=1)

HSP 1 Score: 1135.17 bits (2935), Expect = 0.000e+0
Identity = 699/1278 (54.69%), Postives = 869/1278 (68.00%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCT-IENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS----------------------------ERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNS----IQKDQTGENQKISSQ-----PPVIIHTAATPMPTPRSSP-YNELVXXXXXXXXXXXXXXXQSILSINK----SFETTSSR--------NINTTILQEDAS---------------IMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSI-----PIPYTTNRSNFIIP---SSILLRQGSI--------NSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXX--------XXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEEN 5712
            Q+++K  V    FQR IL+AIL+NTLSMG+EYH QP +LT  LE+SNIVF ++F  EM  K+   G+ GYI NGFN+FDG IV+L ++EL Q G  G SGLS+LRTFRLLRILKLVRFMPALR+QL VM+RT+DNVATFFALL+LF+FIFSILGM+LFGCKFC  +E+  R CDRKNFDSLLW+I+TVFQ+LTQEDWNTVLYNGM +TS+WA+LYF+ALMTFGNYVLFNLLVAILVEGFS+                            +RL E   + + + +     + +  ++  + + + ++ L  S    I+ +    N   +S      PP+I HTAATPM TP+ SP  N +  T +        S  +S LS++     SF   S R        N   +  Q  AS               ++  + +S  +++  +F   H    ++PT +    +  S+      + Y  +  N   P      L  Q S+        NSF       R+  +    +SI  + E  LSRQ+SF S                +R   SL S    + +   NY+       + PD   +  +   R N  ++ D + ++        CP+P+G F +R+ ++ Y+ S +N  R+   H++ +K FD  VL FI LNCITLAMERP IPP S ER FL  TNY+FT +FT EM+IKV +KG  IG  AY KSGWN+MDGFLV ISL+D+FI+I    S +IFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC G ++  V  +N CL+  +NEW NQKYNFDNLGQALMALFVL+SKDGWVQIMYTG+DAVGV+ QP EN+NEW LIYFISFLLLVAFFVLNMFVGVVVENFHKC+E+QE EE+A+RAA        KR     K    +R P YW NYS  R  I++V+ SKYFDLAIA VIGLNVITMA E+H MP+ L  ALK+FN+FFTS F+LE+++K++ALG  RYI D+WNQLD++IVILS+ G+V++E+ + F+PINPTI+RVMRVLRIARVLKLLKMA GIRALLDTVIQALPQVGNLGLLFFLLF+IFAALGVELFGRL+C D  PC GLG+HAHF+NFGMAFLTLFR+ATGDNWNGIMKDTLREKC  S++C ++CCVS +IAP+YFV+FVLMAQFVLVNVVVAVLMKHLEESH+MM DD  L R+    + + A     ++   +E+N
Sbjct:  707 QKRLKYFVESNFFQRSILVAILLNTLSMGVEYHNQPEELTIVLEYSNIVFCVMFGTEMTFKIFAYGLFGYISNGFNVFDGFIVILSIVELAQPGENGASGLSVLRTFRLLRILKLVRFMPALRRQLVVMLRTMDNVATFFALLVLFMFIFSILGMNLFGCKFCRRLEDGSRKCDRKNFDSLLWAIITVFQVLTQEDWNTVLYNGMAKTSTWASLYFVALMTFGNYVLFNLLVAILVEGFSTEDEEKKKEKIKETDAADKDEEDEEEKEKQRLAENNNIDDVQSTKSCLNSVDLDVKNNKEKEKKMLALPPSSAPIIKTESPPPNHGFTSSDGPLNPPLITHTAATPMATPQGSPNENAIKDTNNKLSVSVKRSALKSTLSVDSDKSPSFRGGSPRPSPCLRRTNSRGSHHQRSASWRARNRRLRGDKTSLVVDSLSDSADDVEDDVFSSSH---SQSPTYSAGTPRTPSVNTHGHAVTYVFSDCNGHPPLNNQRALSPQSSLKGRALTPRNSFKSHHTWSRNNSV-GSGRSICNSFE--LSRQNSFTS----------------HRTVNSLGSGNSKDDKSNKNYV-------DLPDVKIALDKEEGRENEADEADPDAIDETEWNCSWCPEPRGCFLERHEYAFYLLSNENSLRKLAHHLISRKWFDNTVLIFIALNCITLAMERPDIPPESVERHFLIYTNYVFTFVFTIEMLIKVTAKGFFIGKHAYFKSGWNVMDGFLVIISLIDIFISITASSSPKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCRGLNVTHVTNRNQCLEDKKNEWVNQKYNFDNLGQALMALFVLASKDGWVQIMYTGLDAVGVDKQPIENYNEWRLIYFISFLLLVAFFVLNMFVGVVVENFHKCRESQEIEERAKRAAKRQEKLDKKRKSEAPKALSEMREPPYWANYSHSRLLIHTVINSKYFDLAIAAVIGLNVITMAMEYHTMPEELTFALKIFNFFFTSVFILESVMKIIALGFLRYIKDRWNQLDILIVILSVVGIVLEEMRTTFIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVIQALPQVGNLGLLFFLLFFIFAALGVELFGRLDC-DREPCEGLGKHAHFKNFGMAFLTLFRVATGDNWNGIMKDTLREKCDRSDNCLKDCCVSPLIAPVYFVVFVLMAQFVLVNVVVAVLMKHLEESHRMMDDDTILLRDALITITKAADNPDSKESGLEEDN 1954          

HSP 2 Score: 388.652 bits (997), Expect = 5.441e-105
Identity = 258/520 (49.62%), Postives = 337/520 (64.81%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPS-------TGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTK-HSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSI-SKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQ-------------IRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQ---HYYAINKYPINFSNIP--------PKASPELSVIDVLNSPRHPNHLISDISSKSVSTETK 1878
            M +I +NC+TL MYQPC D+ C+T +C++++ FD+ IFAFF  EM IK+IAMG  G   Y  D+WNRLD FIV+ G  E + +          N+ L+AIRT+RVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W GVLR RC+L L E+   P     +F  +    ++ +   ++ICS    +GI KC D PP      +     C   AR  +DN+P    SCVNWNQYYT C+   +NPY+GA++FDNIG AWVAIFQVIS+ESWV+IMY+VQDAHSFWDWIYFVALI+IGSFFMINLCLVVIA+QFSETK+RETERM  ER+RF S  CS++ S  EP  CY E++KLI Q+  ++ R+++K Y + +              R     +G    K  +QR        S     +  H H++ +V + S   Q   H+ ++    I + + P        P+ASPE S ID ++SPR PN+L+   S+ S++  ++
Sbjct:    1 MFVIILNCVTLGMYQPCNDE-CDTFRCKLLENFDHFIFAFFAVEMGIKMIAMGVAGKDTYLADSWNRLDCFIVVAGLAEYIVNKTIEYSVNTENLSLSAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWSGVLRRRCFLELPENVSVP-----AFVPL---YYIKSPKIEYICSPFNESGISKCQDFPPF-----HYEGIACNATARNDSDNVPVNGSSCVNWNQYYTSCEERGENPYQGAVSFDNIGLAWVAIFQVISLESWVNIMYFVQDAHSFWDWIYFVALIVIGSFFMINLCLVVIATQFSETKKRETERMLQERKRFQS--CSTLASNSEPAGCYTELLKLIAQMYRRVKRKLIKTYYKTRGMQKINPEKSLSLRRKKSKKKGTNIQKSLQQRFSIVHPHTSSHPATVHHHQHMH-LVPSHSPQPQHPVHHISLVTPDIRYQSPPRPSSTPQAPRASPEQSDIDSMSSPRRPNYLVLPSSNYSLNPSSE 503          

HSP 3 Score: 113.62 bits (283), Expect = 6.144e-21
Identity = 71/279 (25.45%), Postives = 134/279 (48.03%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            N  +++ ++ V+   F   +L  I LN +++ +E  + P   +  + L  +N +F ++F  EM  K+ + GL      Y+ +G+N+ DGF+V +S+++L      G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF  KF                C +      K  + NFD+L  A++ +F + +++ W  ++Y G+               WA +YF++ +    + + N+ V ++VE F
Sbjct:  704 NGMQKRLKYFVESNFFQRSILVAILLNTLSMGVEYHNQP--EELTIVLEYSNIVFCVMFGTEMTFKIFAYGLF----GYISNGFNVFDGFIVILSIVELAQPGENGASG--LSVLRTFRLLRILKLVRFM---PALRRQLVVMLRTMDNVATFFALLVLFMFIFSILGMNLFGCKF----------------CRRLEDGSRKCDRKNFDSLLWAIITVFQVLTQEDWNTVLYNGMAKTST----------WASLYFVALMTFGNYVLFNLLVAILVEGF 945          

HSP 4 Score: 103.219 bits (256), Expect = 7.354e-18
Identity = 92/335 (27.46%), Postives = 150/335 (44.78%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGI-LRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG--------------------KFYYCDGPHLQAV---------------------------KTKND----------CLKYSQ-----NEWKNQKY----NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            +F I LNC+TL M +P        R  L     ++IF   F  EM IK+++ G+  G   YL   WN +D F+V   L +  +   + +S     + L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G                      YY   P ++ +                             +ND          C+ ++Q      E     Y    +FDN+G A +A+F + S + WV IMY   DA          H+ W  IYF++ +++ +FF++N+ + V+   F + ++ +
Sbjct:    1 MFVIILNCVTLGMYQPCNDECDTFRCKLLENFDHFIFAF-FAVEMGIKMIAMGV-AGKDTYLADSWNRLDCFIVVAGLAEYIVNKTIEYSVNTENLSLSAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWSGVLRRRCFLELPENVSVPAFVPLYYIKSPKIEYICSPFNESGISKCQDFPPFHYEGIACNATARNDSDNVPVNGSSCVNWNQYYTSCEERGENPYQGAVSFDNIGLAWVAIFQVISLESWVNIMYFVQDA----------HSFWDWIYFVALIVIGSFFMINLCLVVIATQFSETKKRE 323          

HSP 5 Score: 68.9366 bits (167), Expect = 1.764e-7
Identity = 49/172 (28.49%), Postives = 80/172 (46.51%), Query Frame = 1
Query:  214 GNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLR 726
            G +++ +  D    D  +      P  P    +    YA  F++       R     +I+  WF+   +  IA+NCITL+M +P  D   E+ +   +   +Y+    FT EM+IK+ A GF +G   YF   WN +D F+VI+  I+I       I +TA  + ++   LR
Sbjct: 1284 GRENEADEADPDAIDETEWNCSWCP-EPRGCFLERHEYA--FYLLSNENSLRKLAHHLISRKWFDNTVLIFIALNCITLAMERP--DIPPESVERHFLIYTNYVFTFVFTIEMLIKVTAKGFFIGKHAYFKSGWNVMDGFLVIISLIDIF------ISITASSSPKIFGILR 1444          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. TrEMBL
Match: A0A2C9KE95 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106079304 PE=3 SV=1)

HSP 1 Score: 1117.84 bits (2890), Expect = 0.000e+0
Identity = 689/1248 (55.21%), Postives = 854/1248 (68.43%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCT-IENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS----------------------------ERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNS----IQKDQTGENQKISSQ-----PPVIIHTAATPMPTPRSSP-YNELVXXXXXXXXXXXXXXXQSILSINK----SFETTSSR--------NINTTILQEDAS---------------IMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSI-----PIPYTTNRSNFIIP---SSILLRQGSI--------NSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHS----KERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXX--------XXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHK 5610
            Q+++K  V    FQR IL+AIL+NTLSMG+EYH QP +LT  LE+SNIVF ++F  EM  K+   G+ GYI NGFN+FDG IV+L ++EL Q G  G SGLS+LRTFRLLRILKLVRFMPALR+QL VM+RT+DNVATFFALL+LF+FIFSILGM+LFGCKFC  +E+  R CDRKNFDSLLW+I+TVFQ+LTQEDWNTVLYNGM +TS+WA+LYF+ALMTFGNYVLFNLLVAILVEGFS+                            +RL E   + + + +     + +  ++  + + + ++ L  S    I+ +    N   +S      PP+I HTAATPM TP+ SP  N +  T +        S  +S LS++     SF   S R        N   +  Q  AS               ++  + +S  +++  +F   H    ++PT +    +  S+      + Y  +  N   P      L  Q S+        NSF       R+  +    +SI  + E  LSRQ+SF S                +R   SL S    + +   NY+       + PD   +  +   R N  ++ D + ++        CP+P+G F +R+ ++ Y+ S +N  R+   H++ +K FD  VL FI LNCITLAMERP IPP S    +ER FL  TNY+FT +FT EM+IKV +KG  IG  AY KSGWN+MDGFLV ISL+D+FI+I    S +IFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC G ++  V  +N CL+  +NEW NQKYNFDNLGQALMALFVL+SKDGWVQIMYTG+DAVGV+ QP EN+NEW LIYFISFLLLVAFFVLNMFVGVVVENFHKC+E+QE EE+A+RAA        KR     K    +R P YW NYS  R  I++V+ SKYFDLAIA VIGLNVITMA E+H MP+ L  ALK+FN+FFTS F+LE+++K++ALG  RYI D+WNQLD++IVILS+ G+V++E+ + F+PINPTI+RVMRVLRIARVLKLLKMA GIRALLDTVIQALPQVGNLGLLFFLLF+IFAALGVELFGRL+C D  PC GLG+HAHF+NFGMAFLTLFR+ATGDNWNGIMKDTLREKC  S++C ++CCVS +IAP+YFV+FVLMAQFVLVNVVVAVLMKHLEE++K
Sbjct:  707 QKRLKYFVESNFFQRSILVAILLNTLSMGVEYHNQPEELTIVLEYSNIVFCVMFGTEMTFKIFAYGLFGYISNGFNVFDGFIVILSIVELAQPGENGASGLSVLRTFRLLRILKLVRFMPALRRQLVVMLRTMDNVATFFALLVLFMFIFSILGMNLFGCKFCRRLEDGSRKCDRKNFDSLLWAIITVFQVLTQEDWNTVLYNGMAKTSTWASLYFVALMTFGNYVLFNLLVAILVEGFSTEDEEKKKEKIKETDAADKDEEDEEEKEKQRLAENNNIDDVQSTKSCLNSVDLDVKNNKEKEKKMLALPPSSAPIIKTESPPPNHGFTSSDGPLNPPLITHTAATPMATPQGSPNENAIKDTNNKLSVSVKRSALKSTLSVDSDKSPSFRGGSPRPSPCLRRTNSRGSHHQRSASWRARNRRLRGDKTSLVVDSLSDSADDVEDDVFSSSH---SQSPTYSAGTPRTPSVNTHGHAVTYVFSDCNGHPPLNNQRALSPQSSLKGRALTPRNSFKSHHTWSRNNSV-GSGRSICNSFE--LSRQNSFTS----------------HRTVNSLGSGNSKDDKSNKNYV-------DLPDVKIALDKEEGRENEADEADPDAIDETEWNCSWCPEPRGCFLERHEYAFYLLSNENSLRKLAHHLISRKWFDNTVLIFIALNCITLAMERPDIPPESVVSYEERHFLIYTNYVFTFVFTIEMLIKVTAKGFFIGKHAYFKSGWNVMDGFLVIISLIDIFISITASSSPKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCRGLNVTHVTNRNQCLEDKKNEWVNQKYNFDNLGQALMALFVLASKDGWVQIMYTGLDAVGVDKQPIENYNEWRLIYFISFLLLVAFFVLNMFVGVVVENFHKCRESQEIEERAKRAAKRQEKLDKKRKSEAPKALSEMREPPYWANYSHSRLLIHTVINSKYFDLAIAAVIGLNVITMAMEYHTMPEELTFALKIFNFFFTSVFILESVMKIIALGFLRYIKDRWNQLDILIVILSVVGIVLEEMRTTFIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVIQALPQVGNLGLLFFLLFFIFAALGVELFGRLDC-DREPCEGLGKHAHFKNFGMAFLTLFRVATGDNWNGIMKDTLREKCDRSDNCLKDCCVSPLIAPVYFVVFVLMAQFVLVNVVVAVLMKHLEETYK 1924          

HSP 2 Score: 389.037 bits (998), Expect = 5.136e-105
Identity = 256/519 (49.33%), Postives = 337/519 (64.93%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPS-------TGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTK-HSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSI-SKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNY-----------QNKLKRQRKHCNVCLNSRSQFPIDDHCHLY-SIVNTISGDKQ---HYYAINKYPINFSNIP--------PKASPELSVIDVLNSPRHPNHLISDISSKSVSTETK 1878
            M +I +NC+TL MYQPC D+ C+T +C++++ FD+ IFAFF  EM IK+IAMG  G   Y  D+WNRLD FIV+ G  E + +          N+ L+AIRT+RVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W GVLR RC+L L E+   P     +F  +    ++ +   ++ICS    +GI KC D PP      +     C   AR  +DN+P    SCVNWNQYYT C+   +NPY+GA++FDNIG AWVAIFQVIS+ESWV+IMY+VQDAHSFWDWIYFVALI+IGSFFMINLCLVVIA+QFSETK+RETERM  ER+RF S  CS++ S  EP  CY E++KLI Q+  ++ R+++K Y + +       + +             N  K  ++  ++     S  P   H H +  +V + S   Q   H+ ++    I + + P        P+ASPE S ID ++SPR PN+L+   S+ S++  ++
Sbjct:    1 MFVIILNCVTLGMYQPCNDE-CDTFRCKLLENFDHFIFAFFAVEMGIKMIAMGVAGKDTYLADSWNRLDCFIVVAGLAEYIVNKTIEYSVNTENLSLSAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWSGVLRRRCFLELPENVSVP-----AFVPL---YYIKSPKIEYICSPFNESGISKCQDFPPF-----HYEGIACNATARNDSDNVPVNGSSCVNWNQYYTSCEERGENPYQGAVSFDNIGLAWVAIFQVISLESWVNIMYFVQDAHSFWDWIYFVALIVIGSFFMINLCLVVIATQFSETKKRETERMLQERKRFQS--CSTLASNSEPAGCYTELLKLIAQMYRRVKRKLIKTYYKTRGMQKINPEKSLSLRRKKSKKKGTNIQKSLQQRFSIVHPHTSSHPATVHHHQHMHLVPSHSPQPQHPVHHISLVTPDIRYQSPPRPSSTPQAPRASPEQSDIDSMSSPRRPNYLVLPSSNYSLNPSSE 503          

HSP 3 Score: 113.62 bits (283), Expect = 6.115e-21
Identity = 71/279 (25.45%), Postives = 134/279 (48.03%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            N  +++ ++ V+   F   +L  I LN +++ +E  + P   +  + L  +N +F ++F  EM  K+ + GL      Y+ +G+N+ DGF+V +S+++L      G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF  KF                C +      K  + NFD+L  A++ +F + +++ W  ++Y G+               WA +YF++ +    + + N+ V ++VE F
Sbjct:  704 NGMQKRLKYFVESNFFQRSILVAILLNTLSMGVEYHNQP--EELTIVLEYSNIVFCVMFGTEMTFKIFAYGLF----GYISNGFNVFDGFIVILSIVELAQPGENGASG--LSVLRTFRLLRILKLVRFM---PALRRQLVVMLRTMDNVATFFALLVLFMFIFSILGMNLFGCKF----------------CRRLEDGSRKCDRKNFDSLLWAIITVFQVLTQEDWNTVLYNGMAKTST----------WASLYFVALMTFGNYVLFNLLVAILVEGF 945          

HSP 4 Score: 103.605 bits (257), Expect = 6.726e-18
Identity = 92/335 (27.46%), Postives = 150/335 (44.78%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGI-LRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG--------------------KFYYCDGPHLQAV---------------------------KTKND----------CLKYSQ-----NEWKNQKY----NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            +F I LNC+TL M +P        R  L     ++IF   F  EM IK+++ G+  G   YL   WN +D F+V   L +  +   + +S     + L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G                      YY   P ++ +                             +ND          C+ ++Q      E     Y    +FDN+G A +A+F + S + WV IMY   DA          H+ W  IYF++ +++ +FF++N+ + V+   F + ++ +
Sbjct:    1 MFVIILNCVTLGMYQPCNDECDTFRCKLLENFDHFIFAF-FAVEMGIKMIAMGV-AGKDTYLADSWNRLDCFIVVAGLAEYIVNKTIEYSVNTENLSLSAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWSGVLRRRCFLELPENVSVPAFVPLYYIKSPKIEYICSPFNESGISKCQDFPPFHYEGIACNATARNDSDNVPVNGSSCVNWNQYYTSCEERGENPYQGAVSFDNIGLAWVAIFQVISLESWVNIMYFVQDA----------HSFWDWIYFVALIVIGSFFMINLCLVVIATQFSETKKRE 323          

HSP 5 Score: 64.6994 bits (156), Expect = 3.858e-6
Identity = 50/176 (28.41%), Postives = 80/176 (45.45%), Query Frame = 1
Query:  214 GNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAF----DYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLR 726
            G +++ +  D    D  +      P  P    +    YA  F++       R     +I+  WF+   +  IA+NCITL+M +P  D   E+      + F    +Y+    FT EM+IK+ A GF +G   YF   WN +D F+VI+  I+I       I +TA  + ++   LR
Sbjct: 1284 GRENEADEADPDAIDETEWNCSWCP-EPRGCFLERHEYA--FYLLSNENSLRKLAHHLISRKWFDNTVLIFIALNCITLAMERP--DIPPESVVSYEERHFLIYTNYVFTFVFTIEMLIKVTAKGFFIGKHAYFKSGWNVMDGFLVIISLIDIF------ISITASSSPKIFGILR 1448          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. TrEMBL
Match: A0A2C9KE61 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106079304 PE=3 SV=1)

HSP 1 Score: 1115.14 bits (2883), Expect = 0.000e+0
Identity = 695/1293 (53.75%), Postives = 866/1293 (66.98%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKR------------TCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS----------------------------ERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNS----IQKDQTGENQKISSQ-----PPVIIHTAATPMPTPRSSP-YNELVXXXXXXXXXXXXXXXQSILSINK----SFETTSSR--------NINTTILQEDAS---------------IMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSI-----PIPYTTNRSNFIIP---SSILLRQGSI--------NSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHS----KERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXX--------XXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEEN 5712
            Q+++K  V    FQR IL+AIL+NTLSMG+EYH QP +LT  LE+SNIVF ++F  EM  K+   G+ GYI NGFN+FDG IV+L ++EL Q G  G SGLS+LRTFRLLRILKLVRFMPALR+QL VM+RT+DNVATFFALL+LF+FIFSILGM+LFG  FC +E+                CDR NFD+LLWS+VTVFQ+LTQEDWNTVLYNGM +TS+WA+LYF+ALMTFGNYVLFNLLVAILVEGFS+                            +RL E   + + + +     + +  ++  + + + ++ L  S    I+ +    N   +S      PP+I HTAATPM TP+ SP  N +  T +        S  +S LS++     SF   S R        N   +  Q  AS               ++  + +S  +++  +F   H    ++PT +    +  S+      + Y  +  N   P      L  Q S+        NSF       R+  +    +SI  + E  LSRQ+SF S                +R   SL S    + +   NY+       + PD   +  +   R N  ++ D + ++        CP+P+G F +R+ ++ Y+ S +N  R+   H++ +K FD  VL FI LNCITLAMERP IPP S    +ER FL  TNY+FT +FT EM+IKV +KG  IG  AY KSGWN+MDGFLV ISL+D+FI+I    S +IFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG FYYC G ++  V  +N CL+  +NEW NQKYNFDNLGQALMALFVL+SKDGWVQIMYTG+DAVGV+ QP EN+NEW LIYFISFLLLVAFFVLNMFVGVVVENFHKC+E+QE EE+A+RAA        KR     K    +R P YW NYS  R  I++V+ SKYFDLAIA VIGLNVITMA E+H MP+ L  ALK+FN+FFTS F+LE+++K++ALG  RYI D+WNQLD++IVILS+ G+V++E+ + F+PINPTI+RVMRVLRIARVLKLLKMA GIRALLDTVIQALPQVGNLGLLFFLLF+IFAALGVELFGRL+C D  PC GLG+HAHF+NFGMAFLTLFR+ATGDNWNGIMKDTLREKC  S++C ++CCVS +IAP+YFV+FVLMAQFVLVNVVVAVLMKHLEESH+MM DD  L R+    + + A     ++   +E+N
Sbjct:  707 QKRLKYFVESNFFQRSILVAILLNTLSMGVEYHNQPEELTIVLEYSNIVFCVMFGTEMTFKIFAYGLFGYISNGFNVFDGFIVILSIVELAQPGENGASGLSVLRTFRLLRILKLVRFMPALRRQLVVMLRTMDNVATFFALLVLFMFIFSILGMNLFGGSFCEMEDGTACSCKERCNASLCKCDRANFDNLLWSLVTVFQVLTQEDWNTVLYNGMAKTSTWASLYFVALMTFGNYVLFNLLVAILVEGFSTEDEEKKKEKIKETDAADKDEEDEEEKEKQRLAENNNIDDVQSTKSCLNSVDLDVKNNKEKEKKMLALPPSSAPIIKTESPPPNHGFTSSDGPLNPPLITHTAATPMATPQGSPNENAIKDTNNKLSVSVKRSALKSTLSVDSDKSPSFRGGSPRPSPCLRRTNSRGSHHQRSASWRARNRRLRGDKTSLVVDSLSDSADDVEDDVFSSSH---SQSPTYSAGTPRTPSVNTHGHAVTYVFSDCNGHPPLNNQRALSPQSSLKGRALTPRNSFKSHHTWSRNNSV-GSGRSICNSFE--LSRQNSFTS----------------HRTVNSLGSGNSKDDKSNKNYV-------DLPDVKIALDKEEGRENEADEADPDAIDETEWNCSWCPEPRGCFLERHEYAFYLLSNENSLRKLAHHLISRKWFDNTVLIFIALNCITLAMERPDIPPESVVSYEERHFLIYTNYVFTFVFTIEMLIKVTAKGFFIGKHAYFKSGWNVMDGFLVIISLIDIFISITASSSPKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCRGLNVTHVTNRNQCLEDKKNEWVNQKYNFDNLGQALMALFVLASKDGWVQIMYTGLDAVGVDKQPIENYNEWRLIYFISFLLLVAFFVLNMFVGVVVENFHKCRESQEIEERAKRAAKRQEKLDKKRKSEAPKALSEMREPPYWANYSHSRLLIHTVINSKYFDLAIAAVIGLNVITMAMEYHTMPEELTFALKIFNFFFTSVFILESVMKIIALGFLRYIKDRWNQLDILIVILSVVGIVLEEMRTTFIPINPTIIRVMRVLRIARVLKLLKMAKGIRALLDTVIQALPQVGNLGLLFFLLFFIFAALGVELFGRLDC-DREPCEGLGKHAHFKNFGMAFLTLFRVATGDNWNGIMKDTLREKCDRSDNCLKDCCVSPLIAPVYFVVFVLMAQFVLVNVVVAVLMKHLEESHRMMDDDTILLRDALITITKAADNPDSKESGLEEDN 1969          

HSP 2 Score: 388.652 bits (997), Expect = 5.546e-105
Identity = 258/520 (49.62%), Postives = 337/520 (64.81%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPS-------TGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTK-HSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSI-SKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQ-------------IRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQ---HYYAINKYPINFSNIP--------PKASPELSVIDVLNSPRHPNHLISDISSKSVSTETK 1878
            M +I +NC+TL MYQPC D+ C+T +C++++ FD+ IFAFF  EM IK+IAMG  G   Y  D+WNRLD FIV+ G  E + +          N+ L+AIRT+RVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W GVLR RC+L L E+   P     +F  +    ++ +   ++ICS    +GI KC D PP      +     C   AR  +DN+P    SCVNWNQYYT C+   +NPY+GA++FDNIG AWVAIFQVIS+ESWV+IMY+VQDAHSFWDWIYFVALI+IGSFFMINLCLVVIA+QFSETK+RETERM  ER+RF S  CS++ S  EP  CY E++KLI Q+  ++ R+++K Y + +              R     +G    K  +QR        S     +  H H++ +V + S   Q   H+ ++    I + + P        P+ASPE S ID ++SPR PN+L+   S+ S++  ++
Sbjct:    1 MFVIILNCVTLGMYQPCNDE-CDTFRCKLLENFDHFIFAFFAVEMGIKMIAMGVAGKDTYLADSWNRLDCFIVVAGLAEYIVNKTIEYSVNTENLSLSAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWSGVLRRRCFLELPENVSVP-----AFVPL---YYIKSPKIEYICSPFNESGISKCQDFPPF-----HYEGIACNATARNDSDNVPVNGSSCVNWNQYYTSCEERGENPYQGAVSFDNIGLAWVAIFQVISLESWVNIMYFVQDAHSFWDWIYFVALIVIGSFFMINLCLVVIATQFSETKKRETERMLQERKRFQS--CSTLASNSEPAGCYTELLKLIAQMYRRVKRKLIKTYYKTRGMQKINPEKSLSLRRKKSKKKGTNIQKSLQQRFSIVHPHTSSHPATVHHHQHMH-LVPSHSPQPQHPVHHISLVTPDIRYQSPPRPSSTPQAPRASPEQSDIDSMSSPRRPNYLVLPSSNYSLNPSSE 503          

HSP 3 Score: 119.013 bits (297), Expect = 1.264e-22
Identity = 75/279 (26.88%), Postives = 139/279 (49.82%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            N  +++ ++ V+   F   +L  I LN +++ +E  + P   +  + L  +N +F ++F  EM  K+ + GL      Y+ +G+N+ DGF+V +S+++L      G S     +LR FRLLR L+ +R +   P L+  +  +L ++  +     +   F  IF ILG+ LF G F  C+     A   K  C   + +  K  + NFDNL  +L+ +F + +++ W  ++Y G+               WA +YF++ +    + + N+ V ++VE F
Sbjct:  704 NGMQKRLKYFVESNFFQRSILVAILLNTLSMGVEYHNQP--EELTIVLEYSNIVFCVMFGTEMTFKIFAYGLF----GYISNGFNVFDGFIVILSIVELAQPGENGASG--LSVLRTFRLLRILKLVRFM---PALRRQLVVMLRTMDNVATFFALLVLFMFIFSILGMNLFGGSF--CEMEDGTACSCKERC---NASLCKCDRANFDNLLWSLVTVFQVLTQEDWNTVLYNGMAKTST----------WASLYFVALMTFGNYVLFNLLVAILVEGF 956          

HSP 4 Score: 103.605 bits (257), Expect = 5.819e-18
Identity = 92/335 (27.46%), Postives = 150/335 (44.78%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPHSKER--LFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGI-LRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG--------------------KFYYCDGPHLQAV---------------------------KTKND----------CLKYSQ-----NEWKNQKY----NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            +F I LNC+TL M +P        R  L     ++IF   F  EM IK+++ G+  G   YL   WN +D F+V   L +  +   + +S     + L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G                      YY   P ++ +                             +ND          C+ ++Q      E     Y    +FDN+G A +A+F + S + WV IMY   DA          H+ W  IYF++ +++ +FF++N+ + V+   F + ++ +
Sbjct:    1 MFVIILNCVTLGMYQPCNDECDTFRCKLLENFDHFIFAF-FAVEMGIKMIAMGV-AGKDTYLADSWNRLDCFIVVAGLAEYIVNKTIEYSVNTENLSLSAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWSGVLRRRCFLELPENVSVPAFVPLYYIKSPKIEYICSPFNESGISKCQDFPPFHYEGIACNATARNDSDNVPVNGSSCVNWNQYYTSCEERGENPYQGAVSFDNIGLAWVAIFQVISLESWVNIMYFVQDA----------HSFWDWIYFVALIVIGSFFMINLCLVVIATQFSETKKRE 323          

HSP 5 Score: 64.6994 bits (156), Expect = 4.019e-6
Identity = 50/176 (28.41%), Postives = 80/176 (45.45%), Query Frame = 1
Query:  214 GNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAF----DYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLR 726
            G +++ +  D    D  +      P  P    +    YA  F++       R     +I+  WF+   +  IA+NCITL+M +P  D   E+      + F    +Y+    FT EM+IK+ A GF +G   YF   WN +D F+VI+  I+I       I +TA  + ++   LR
Sbjct: 1295 GRENEADEADPDAIDETEWNCSWCP-EPRGCFLERHEYA--FYLLSNENSLRKLAHHLISRKWFDNTVLIFIALNCITLAMERP--DIPPESVVSYEERHFLIYTNYVFTFVFTIEMLIKVTAKGFFIGKHAYFKSGWNVMDGFLVIISLIDIF------ISITASSSPKIFGILR 1459          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1ha (calcium channel, voltage-dependent, T type, alpha 1H subunit a [Source:ZFIN;Acc:ZDB-GENE-130103-1])

HSP 1 Score: 623.624 bits (1607), Expect = 0.000e+0
Identity = 395/678 (58.26%), Postives = 512/678 (75.52%), Query Frame = 1
Query: 3673 KALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHK------MMCDDYELEREVQRDLDEEAKE 5676
            +A++ P D  +E      +N  +  +EED++   C  L +     K  + K +   SLY+FS +N+FR  C  I+  + FD++VL FI LNCIT+A+ERP+I PHS ER+FL+++NY+FT+IF  EM +KV++    +G  +YL+S WNI+DG LVF+SL+D+ +++     NRI GILRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIV+IC  FFIIFGILGVQLFKGKFY+C+G   + +  K+DCL  +  +W  +KYNFDNLGQALM+LFVLS KDGWV IMY G+DAVGV+ QP  NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+EE   R  KR R+ EK+R+  +   Y+  YS  R  I+++ ++ Y DL I  +I +NV+TM+ E +  PQ L+ ALK  NY FT  F+LEA+LKLVA G+ R+  D+WNQLD+ IV+LSI G+ ++E++ N  +PINPTI+R+MRVLRIARVLKLLKMA G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L+C    PC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE CI +++C        +++P+YFV FVLMAQFVLVNVVVAVLMKHLEES+K       M  D E+++E+Q D + +  +
Sbjct: 1089 RAMQLPTDLPAE------LNREDSANEEDLDDSFCCRLRRMLNAYKPQWCKDHEDWSLYMFSPENKFRVICNRIISHRLFDHVVLVFIFLNCITIALERPNIKPHSTERVFLSISNYVFTVIFLAEMTVKVVA----LGFYSYLQSTWNILDGMLVFVSLIDILVSLASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVVICWAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCL-LANYKWVPRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRRRQQEKRRRKAQKIPYYAYYSPIRLYIHTLCSNHYLDLFITFIICINVVTMSIEHYDQPQYLEEALKYCNYVFTIFFILEAILKLVAFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNAALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCIK-QPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE-CIPADNC---LSYLPLVSPLYFVTFVLMAQFVLVNVVVAVLMKHLEESNKEAKEEAEMDSDTEMDKEIQNDEERQGTQ 1750          

HSP 2 Score: 352.058 bits (902), Expect = 1.397e-97
Identity = 211/393 (53.69%), Postives = 282/393 (71.76%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGN 1596
            M +I +NC+TL MYQPC D  C++  C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N  L+AIRT+RVLRPL+AINR+PSM+ILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC++  N +  +  L++S +Y        +  D  FICS++K NG+ +C ++PP   L+ N            +T + P  +SCVNWNQYY  C+PG+ NP++GA+NFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+R  + M  +R  + S++ +  S  EP +CY E+++ I+ +  K+ RR  +LY+  Q +  K    N
Sbjct:    1 MLVILLNCVTLGMYQPCEDVACQSEWCIILRAFDDCIFAFFAVEMVIKMMALGIFGPACYLGDTWNRLDFFIVMAGMLE-YSLDGHNASLSAIRTVRVLRPLKAINRVPSMKILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMKENFTDIY-NLSMSQYYMDE-----DGEDNPFICSSEKENGMRRCRNVPP---LMENGFNCTLNASFSHYTYSDP--NSCVNWNQYYNQCRPGDLNPHKGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRVNKLMKDQRVCYQSNDSTLASYSEPGSCYEEMLRYISHLYRKVKRRTSRLYHGWQSKRRKKINPN 381          

HSP 3 Score: 325.479 bits (833), Expect = 2.869e-89
Identity = 183/320 (57.19%), Postives = 234/320 (73.12%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE------RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSS 3093
            + ++K +V+   F RGI+IAILINTLSMGIE+H+QP +LT  LE SNIVF+ +F++EMI KL+  GI GYIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V+++++DNVATF  LL+LFIF FSILGM LFGCKFC   EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  +S  A+LYF+ALMTFGNYVLFNLLVAILVE F +E        DE+K   ++ +  K      ++L++        ++++ +   +  G     +  PP+I+ TAATPMPTP+SS
Sbjct:  648 RDRMKRIVDSKYFNRGIMIAILINTLSMGIEHHEQPKELTDILEISNIVFTSMFVLEMIFKLVAFGIYGYIKNPYNIFDGIIVVISVWEIIGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKSMDNVATFCMLLMLFIFTFSILGMHLFGCKFCLKTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASSSPLASLYFVALMTFGNYVLFNLLVAILVEAFQAEGDANRSDSDEEKRSVDFEEEEKMGELRARELKMYS------LMMSANGHVESRG-----NLPPPIIMRTAATPMPTPKSS 955          

HSP 4 Score: 113.62 bits (283), Expect = 1.993e-24
Identity = 75/293 (25.60%), Postives = 143/293 (48.81%), Query Frame = 1
Query: 3817 KRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            KR    +++      FR + + IVD K F+  ++  I +N +++ +E    P    +   L ++N +FT +F  EMI K+++ G+      Y+K+ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ S+  +    ++   F   F ILG+ LF  KF                CLK    +    + NFD+L  A++ +F + +++ W  ++Y G+           + +  A +YF++ +    + + N+ V ++VE F 
Sbjct:  632 KRLCERIHMARYWEDFRDRMKRIVDSKYFNRGIMIAILINTLSMGIEHHEQPKELTD--ILEISNIVFTSMFVLEMIFKLVAFGIY----GYIKNPYNIFDGIIVVISVWE-----IIGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMKSMDNVATFCMLLMLFIFTFSILGMHLFGCKF----------------CLKTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM----------ASSSPLASLYFVALMTFGNYVLFNLLVAILVEAFQ 884          

HSP 5 Score: 88.5817 bits (218), Expect = 7.369e-17
Identity = 60/212 (28.30%), Postives = 111/212 (52.36%), Query Frame = 1
Query: 2140 YLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            Y S  +  I TL ++      I   I IN ++M IE++ QP  L + L++ N VF+I F++E ILKL+  GI  + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  +  +       F++   + +T+F++ T ++WN ++
Sbjct: 1458 YYSPIRLYIHTLCSNHYLDLFITFIICINVVTMSIEHYDQPQYLEEALKYCNYVFTIFFILEAILKLVAFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNAALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCIKQPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIM 1669          

HSP 6 Score: 81.2629 bits (199), Expect = 1.113e-14
Identity = 89/303 (29.37%), Postives = 156/303 (51.49%), Query Frame = 1
Query: 4795 YWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLE-ESHKMMCDDYELEREVQRDLDEEAKELAREKQLK 5700
            YWE++   R  +  +V SKYF+  I   I +N ++M  E H  P+ L   L++ N  FTS FVLE + KLVA G+  YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++++  V    +L  L  + F+ LG+ LFG   C        +    +F++   A +T+F+I T ++WN ++ + +                S+ +A +YFV  +    +VL N++VA+L++  + E      D  E +R V  + +E+  EL R ++LK
Sbjct:  643 YWEDF---RDRMKRIVDSKYFNRGIMIAILINTLSMGIEHHEQPKELTDILEISNIVFTSMFVLEMIFKLVAFGIYGYIKNPYNIFDGIIVVISVWE-IIGQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMKSMDNVATFCMLLMLFIFTFSILGMHLFGCKFCLKTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAS--------------SSPLASLYFVALMTFGNYVLFNLLVAILVEAFQAEGDANRSDSDEEKRSVDFEEEEKMGEL-RARELK 918          

HSP 7 Score: 79.7221 bits (195), Expect = 4.000e-14
Identity = 68/266 (25.56%), Postives = 130/266 (48.87%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDE 2883
            F   +L+ I +N +++ +E        T+   L  SN VF+++FL EM +K++  G   Y+++ +N+ DG++V + +I++     +SG       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C + N K    + NFD+L  +++++F +  ++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F   R D+
Sbjct: 1162 FDHVVLVFIFLNCITIALERPNIKPHSTERVFLSISNYVFTVIFLAEMTVKVVALGFYSYLQSTWNILDGMLVFVSLIDILVSLASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVVICWAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLLANYKWVPRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQ 1427          

HSP 8 Score: 78.1814 bits (191), Expect = 1.185e-13
Identity = 56/156 (35.90%), Postives = 88/156 (56.41%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M +P   +   S+  + L   +      F  EM+IK+++ G+  G   YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPL+ I+R P +K++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:    1 MLVILLNCVTLGMYQPCEDVACQSEWCIILRAFDDCIFAFFAVEMVIKMMALGIF-GPACYLGDTWNRLDFFIVMAGMLEYSLD---GHNASLSAI----RTVRVLRPLKAINRVPSMKILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 148          

HSP 9 Score: 55.8398 bits (133), Expect = 6.222e-7
Identity = 33/116 (28.45%), Postives = 60/116 (51.72%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C +II+   F+ + +  I +NCIT+++ +P  +    +++   +   +Y+    F  EM +K++A+GF     Y   TWN LD  +V V  I+IL S  ++ G
Sbjct: 1140 LYMFSPENKFRVICNRIISHRLFDHVVLVFIFLNCITIALERP--NIKPHSTERVFLSISNYVFTVIFLAEMTVKVVALGF---YSYLQSTWNILDGMLVFVSLIDILVSLASSGG 1250          

HSP 10 Score: 53.1434 bits (126), Expect = 4.455e-6
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE 4707
            N C     N  K    NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++
Sbjct:  233 NQCRPGDLNPHKG-AVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQ 315          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1ha (calcium channel, voltage-dependent, T type, alpha 1H subunit a [Source:ZFIN;Acc:ZDB-GENE-130103-1])

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 391/684 (57.16%), Postives = 506/684 (73.98%), Query Frame = 1
Query: 3673 KALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMIC--LSKCCPQPKGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ-------XXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESN-FMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHK------MMCDDYELEREVQRDLDEEAK 5673
            +A++ P D  +E      +N  +  +EED++   C  L +     K  + K +   SLY+FS +N+FR  C  I+  + FD++VL FI LNCIT+A+ERP+I PHS ER+FL+++NY+FT+IF  EM +KV++ G      +YL+S WNI+DG LVF+SL+D+ +++     NRI GILRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIV+IC  FFIIFGILGVQLFKGKFY+C+G   + +  K+DCL  +  +W  +KYNFDNLGQALM+LFVLS KDGWV IMY G+DAVGV+ QP  NHN W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++Q        +E++ R+  KR R  E      +   Y+  YS  R  I+++ ++ Y DL I  +I +NV+TM+ E +  PQ L+ ALK  NY FT  F+LEA+LKLVA G+ R+  D+WNQLD+ IV+LSI G+ ++E++ N  +PINPTI+R+MRVLRIARVLKLLKMA G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L+C    PC GL RHA FENFGMAFLTLFR++TGDNWNGIMKDTLRE CI +++C        +++P+YFV FVLMAQFVLVNVVVAVLMKHLEES+K       M  D E+++E+Q   D+  +
Sbjct: 1077 RAMQLPTDLPAE------LNREDSANEEDLDDSFCCRLRRMLNAYKPQWCKDHEDWSLYMFSPENKFRVICNRIISHRLFDHVVLVFIFLNCITIALERPNIKPHSTERVFLSISNYVFTVIFLAEMTVKVVALGFY----SYLQSTWNILDGMLVFVSLIDILVSLASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVVICWAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCL-LANYKWVPRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREEKRRRQQEKRRRSKENYGSEAQKIPYYAYYSPIRLYIHTLCSNHYLDLFITFIICINVVTMSIEHYDQPQYLEEALKYCNYVFTIFFILEAILKLVAFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNAALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCIK-QPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIMKDTLRE-CIPADNC---LSYLPLVSPLYFVTFVLMAQFVLVNVVVAVLMKHLEESNKEAKEEAEMDSDTEMDKEIQNATDDSGE 1744          

HSP 2 Score: 352.829 bits (904), Expect = 1.760e-97
Identity = 211/393 (53.69%), Postives = 282/393 (71.76%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGN 1596
            M +I +NC+TL MYQPC D  C++  C I++AFD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E     G+N  L+AIRT+RVLRPL+AINR+PSM+ILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC++  N +  +  L++S +Y        +  D  FICS++K NG+ +C ++PP   L+ N            +T + P  +SCVNWNQYY  C+PG+ NP++GA+NFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+R  + M  +R  + S++ +  S  EP +CY E+++ I+ +  K+ RR  +LY+  Q +  K    N
Sbjct:    1 MLVILLNCVTLGMYQPCEDVACQSEWCIILRAFDDCIFAFFAVEMVIKMMALGIFGPACYLGDTWNRLDFFIVMAGMLE-YSLDGHNASLSAIRTVRVLRPLKAINRVPSMKILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFMKENFTDIY-NLSMSQYYMDE-----DGEDNPFICSSEKENGMRRCRNVPP---LMENGFNCTLNASFSHYTYSDP--NSCVNWNQYYNQCRPGDLNPHKGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRVNKLMKDQRVCYQSNDSTLASYSEPGSCYEEMLRYISHLYRKVKRRTSRLYHGWQSKRRKKINPN 381          

HSP 3 Score: 326.635 bits (836), Expect = 2.153e-89
Identity = 183/320 (57.19%), Postives = 234/320 (73.12%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFC-TIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE------RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSS 3093
            + ++K +V+   F RGI+IAILINTLSMGIE+H+QP +LT  LE SNIVF+ +F++EMI KL+  GI GYIKN +N+FDGIIVV+ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V+++++DNVATF  LL+LFIF FSILGM LFGCKFC   EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  +S  A+LYF+ALMTFGNYVLFNLLVAILVE F +E        DE+K   ++ +  K      ++L++        ++++ +   +  G     +  PP+I+ TAATPMPTP+SS
Sbjct:  636 RDRMKRIVDSKYFNRGIMIAILINTLSMGIEHHEQPKELTDILEISNIVFTSMFVLEMIFKLVAFGIYGYIKNPYNIFDGIIVVISVWEIIGQA-DGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKSMDNVATFCMLLMLFIFTFSILGMHLFGCKFCLKTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASSSPLASLYFVALMTFGNYVLFNLLVAILVEAFQAEGDANRSDSDEEKRSVDFEEEEKMGELRARELKMYS------LMMSANGHVESRG-----NLPPPIIMRTAATPMPTPKSS 943          

HSP 4 Score: 115.546 bits (288), Expect = 5.505e-25
Identity = 75/293 (25.60%), Postives = 143/293 (48.81%), Query Frame = 1
Query: 3817 KRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            KR    +++      FR + + IVD K F+  ++  I +N +++ +E    P    +   L ++N +FT +F  EMI K+++ G+      Y+K+ +NI DG +V IS+ +     ++G ++    +LR FRLLR L+ +R +   P L+  +  L+ S+  +    ++   F   F ILG+ LF  KF                CLK    +    + NFD+L  A++ +F + +++ W  ++Y G+           + +  A +YF++ +    + + N+ V ++VE F 
Sbjct:  620 KRLCERIHMARYWEDFRDRMKRIVDSKYFNRGIMIAILINTLSMGIEHHEQPKELTD--ILEISNIVFTSMFVLEMIFKLVAFGIY----GYIKNPYNIFDGIIVVISVWE-----IIGQADGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMKSMDNVATFCMLLMLFIFTFSILGMHLFGCKF----------------CLKTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM----------ASSSPLASLYFVALMTFGNYVLFNLLVAILVEAFQ 872          

HSP 5 Score: 90.1225 bits (222), Expect = 2.914e-17
Identity = 61/224 (27.23%), Postives = 113/224 (50.45%), Query Frame = 1
Query: 2104 FNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            +     K     Y S  +  I TL ++      I   I IN ++M IE++ QP  L + L++ N VF+I F++E ILKL+  GI  + K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C  +  +       F++   + +T+F++ T ++WN ++
Sbjct: 1441 YGSEAQKIPYYAYYSPIRLYIHTLCSNHYLDLFITFIICINVVTMSIEHYDQPQYLEEALKYCNYVFTIFFILEAILKLVAFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNAALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLDCIKQPCEGLSRHATFENFGMAFLTLFRVSTGDNWNGIM 1664          

HSP 6 Score: 80.8777 bits (198), Expect = 1.585e-14
Identity = 89/303 (29.37%), Postives = 156/303 (51.49%), Query Frame = 1
Query: 4795 YWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLE-ESHKMMCDDYELEREVQRDLDEEAKELAREKQLK 5700
            YWE++   R  +  +V SKYF+  I   I +N ++M  E H  P+ L   L++ N  FTS FVLE + KLVA G+  YI + +N  D +IV++S+   +I + +          + V+R  R+ RVLKL++    +R  L  +++++  V    +L  L  + F+ LG+ LFG   C        +    +F++   A +T+F+I T ++WN ++ + +                S+ +A +YFV  +    +VL N++VA+L++  + E      D  E +R V  + +E+  EL R ++LK
Sbjct:  631 YWEDF---RDRMKRIVDSKYFNRGIMIAILINTLSMGIEHHEQPKELTDILEISNIVFTSMFVLEMIFKLVAFGIYGYIKNPYNIFDGIIVVISVWE-IIGQADGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMKSMDNVATFCMLLMLFIFTFSILGMHLFGCKFCLKTENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAS--------------SSPLASLYFVALMTFGNYVLFNLLVAILVEAFQAEGDANRSDSDEEKRSVDFEEEEKMGEL-RARELK 906          

HSP 7 Score: 80.1073 bits (196), Expect = 2.808e-14
Identity = 68/266 (25.56%), Postives = 130/266 (48.87%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQT--LEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF----------------CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDE 2883
            F   +L+ I +N +++ +E        T+   L  SN VF+++FL EM +K++  G   Y+++ +N+ DG++V + +I++     +SG       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF                C + N K    + NFD+L  +++++F +  ++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F   R D+
Sbjct: 1150 FDHVVLVFIFLNCITIALERPNIKPHSTERVFLSISNYVFTVIFLAEMTVKVVALGFYSYLQSTWNILDGMLVFVSLIDILVSLASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVVICWAFFIIFGILGVQLFKGKFYHCEGLDTRNITNKSDCLLANYKWVPRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQ 1415          

HSP 8 Score: 78.1814 bits (191), Expect = 1.025e-13
Identity = 56/156 (35.90%), Postives = 88/156 (56.41%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M +P   +   S+  + L   +      F  EM+IK+++ G+  G   YL   WN +D F+V   +L+  +    GH+  +  I    R +R LRPL+ I+R P +K++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:    1 MLVILLNCVTLGMYQPCEDVACQSEWCIILRAFDDCIFAFFAVEMVIKMMALGIF-GPACYLGDTWNRLDFFIVMAGMLEYSLD---GHNASLSAI----RTVRVLRPLKAINRVPSMKILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 148          

HSP 9 Score: 56.225 bits (134), Expect = 4.561e-7
Identity = 33/116 (28.45%), Postives = 60/116 (51.72%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F      R  C +II+   F+ + +  I +NCIT+++ +P  +    +++   +   +Y+    F  EM +K++A+GF     Y   TWN LD  +V V  I+IL S  ++ G
Sbjct: 1128 LYMFSPENKFRVICNRIISHRLFDHVVLVFIFLNCITIALERP--NIKPHSTERVFLSISNYVFTVIFLAEMTVKVVALGF---YSYLQSTWNILDGMLVFVSLIDILVSLASSGG 1238          

HSP 10 Score: 53.9138 bits (128), Expect = 2.533e-6
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE 4707
            N C     N  K    NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++
Sbjct:  233 NQCRPGDLNPHKG-AVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQ 315          

HSP 11 Score: 52.373 bits (124), Expect = 7.062e-6
Identity = 25/80 (31.25%), Postives = 47/80 (58.75%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            NFDN+G A +++F +   + WV+IMY   DA          H+ W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1336 NFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQ 1415          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: cacna1ib (calcium voltage-gated channel subunit alpha1 Ib [Source:ZFIN;Acc:ZDB-GENE-120215-18])

HSP 1 Score: 603.594 bits (1555), Expect = 8.855e-180
Identity = 367/613 (59.87%), Postives = 471/613 (76.84%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNF-MPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQV-GNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDE 5664
            S+Y+FS  N+FR  CQ I+  K FDY+VL FI LNCIT+A+ERP I   S ER+FLT++NYIFT IF  EM +KV+S GL +G++AYL+S WNI+DGFLVF+SL+D+ +++  G   +I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SL+PIGNIVLICC FFIIFGILGVQLFKGKFYYC G  ++ +  K+DCL  ++ +W + KYNFDNLGQALM+LFVL+SKDGWV IMY G+DAVG++ QP  N+N W L+YFISFLL+V+FFVLNMFVGVVVENFHKC+++QE EE  RR  KR RR+EKKR+  +   Y+  YS  R  I+S  TS Y DL I  +I +NV+TM+ E +  P+     LK  NYFF+STFV+EA+LKL+A G  R+  D+WNQLD+ IV+LS+ G+ ++E+E N  +PINPTI+R+MRVLRIARVLKLLKMA+G+RALLDTV+QALPQV   L L    +++   +    L   L C+  +PC G+ RHA FENFGMAFLTLF+++TGDNWNGIMKDTLRE C    +   N  +   I+P+YFV FVL AQFVL+NVVVAVLMKHL++S+K   +D E++ E++ +L +
Sbjct: 1061 SVYLFSPQNKFRLTCQKIIAHKLFDYVVLAFIFLNCITVALERPKIHQGSLERVFLTISNYIFTAIFVAEMTVKVVSMGLYLGERAYLRSSWNILDGFLVFVSLIDIVVSMAGG--AKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCVGLDVKNITNKSDCLS-AKYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVGIDQQPVINNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRLRRMEKKRRKAQKLPYYAGYSHIRLMIHSFCTSHYLDLFITLIICINVVTMSLEHYDQPK----GLKYCNYFFSSTFVVEAMLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVHAGLALFPSFIYHHLTSSFFSLSLYLVCNADHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRE-CPHGNNHSCNASLQ-FISPMYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELELAQ 1664          

HSP 2 Score: 305.449 bits (781), Expect = 6.155e-83
Identity = 211/464 (45.47%), Postives = 275/464 (59.27%), Query Frame = 1
Query:  235 LTDKSKADHCQQETDGTPINPNESLI-YHGFYATVFHIFHQNKHPRDWCLKIITWP------WFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSL--------QICKQPARPF--TDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR-FNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIR 1572
            L  + +A H ++  D      +  ++ Y      VF    Q   PR WC+++++ P      WFE++SM +I +NC+TL MYQPC + +C + +CQI+QAFD +I+ FF  EMV+K++ +G  G   Y GDTWNRLD FIV+ G +E       NI  TAIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRCY   N +    G+ L + Y           ++ FICS  ++NGI+ CSDIP       N  L        Q     A P            CVNWN+YYT C  G KNP++GAINFDNIG+AW+ IFQV +                    IYF    ++GSFFMINLCLVVIA+QFSETK+RE + M  +R    +SS  +S++  EP  CY E+ +L   I+ K  RR   L+   + R
Sbjct:   29 LEPEERAGHVEEVDDDVSSELSGLVVPYPELAPVVFFCLKQTTFPRSWCIRLVSSPYPLLFTWFERVSMMVIILNCVTLGMYQPCENIDCSSQRCQILQAFDAMIYIFFAVEMVVKMVGLGIFGRRCYLGDTWNRLDFFIVMAGVVEYSLDL-QNINFTAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEDNFTLSS-GMALPTPYYSPD----EEDERPFICSLPQDNGIMSCSDIPVRREGGRNCCLDVEDALHWQALGMAAEPIFNASAGAVPGLCVNWNRYYTRCLTGYKNPHKGAINFDNIGYAWIVIFQVFT--------------------IYFFFYQMVGSFFMINLCLVVIATQFSETKQREHQLMQEQRAACLSSSTLASLA--EPGDCYEELFQLACHILRKARRRSAALFRAFRRR 464          

HSP 3 Score: 292.352 bits (747), Expect = 5.935e-79
Identity = 164/267 (61.42%), Postives = 199/267 (74.53%), Query Frame = 1
Query: 2107 NKNQNKTKRKNYLSICQQKIKTL-----------VNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE 2871
            ++  ++++ K     C+ K K L           V    F RGI+IAILINT+SMGIE+H+QP +LT  LE  NIVF+ +F +EMILKL   G   Y++N +N+FDGIIV++ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIFIFSILGM +FGCKF    +   T  DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS  AALYF+ALMTFGNYVLFNLLVAILVEGF +E
Sbjct:  538 DEQADQSEEKKEKGACKGKCKELWIEMRIKLWGIVESKYFNRGIMIAILINTISMGIEHHEQPEELTNVLEICNIVFTSMFALEMILKLTAFGCFNYLRNPYNIFDGIIVIISVCEIVGQS-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTDAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAE 803          

HSP 4 Score: 106.686 bits (265), Expect = 2.268e-22
Identity = 86/342 (25.15%), Postives = 154/342 (45.03%), Query Frame = 1
Query: 3676 ALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERL--FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            A EE +D   +     + +   D  EE  E   C  KC               L++     + R K   IV+ K F+  ++  I +N I++ +E    P    E L   L + N +FT +F  EMI+K+ + G       YL++ +NI DG +V IS+ +     ++G S+    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ +F  KF                 LK    +    + NFD+L  A++ +F + +++ W  ++Y G+ +           +  A +YF++ +    + + N+ V ++VE F 
Sbjct:  525 ASEEAEDGAEQ-----KTDEQADQSEEKKEKGACKGKC-------------KELWI-----EMRIKLWGIVESKYFNRGIMIAILINTISMGIEHHEQP----EELTNVLEICNIVFTSMFALEMILKLTAFGCF----NYLRNPYNIFDGIIVIISVCE-----IVGQSDGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKF----------------SLKTDAGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SPLAALYFVALMTFGNYVLFNLLVAILVEGFQ 801          

HSP 5 Score: 76.2554 bits (186), Expect = 3.906e-13
Identity = 65/254 (25.59%), Postives = 121/254 (47.64%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD----RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT------SSWAALYFIALMTF------GNYVLFNLLVAILVEGFSSERLDEQK 2889
            I + I IN ++M +E++ QP    + L++ N  FS  F++E +LKLI  G   + K+ +N  D  IV+L V+ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V    AL   FI+       S F      + N    C+       F++   + +T+FQ+ T ++WN ++ + +         S  A+L FI+ M F        +VL N++VA+L++       + Q+
Sbjct: 1403 ITLIICINVVTMSLEHYDQP----KGLKYCNYFFSSTFVVEAMLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEINASLPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVHAGLALFPSFIYHHLTS--SFFSLSLYLVCNADHPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRECPHGNNHSCNASLQFISPMYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQE 1650          

HSP 6 Score: 75.485 bits (184), Expect = 7.278e-13
Identity = 55/161 (34.16%), Postives = 92/161 (57.14%), Query Frame = 1
Query: 3901 FDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            F+ + +  I LNC+TL M +P  +I   S+    L   + +  I F  EM++K++  G+  G + YL   WN +D F+V   +++  + +     N  F  +R    +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   92 FERVSMMVIILNCVTLGMYQPCENIDCSSQRCQILQAFDAMIYIFFAVEMVVKMVGLGIF-GRRCYLGDTWNRLDFFIVMAGVVEYSLDL----QNINFTAIRT---VRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 244          

HSP 7 Score: 71.2478 bits (173), Expect = 1.477e-11
Identity = 66/222 (29.73%), Postives = 113/222 (50.90%), Query Frame = 1
Query: 4801 ENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            E + + R  +  +V SKYF+  I   I +N I+M  E H  P+ L   L++ N  FTS F LE +LKL A G   Y+ + +N  D +IVI+S+  +V               + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ +FG     + +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  559 ELWIEMRIKLWGIVESKYFNRGIMIAILINTISMGIEHHEQPEELTNVLEICNIVFTSMFALEMILKLTAFGCFNYLRNPYNIFDGIIVIISVCEIVGQSDGG---------LSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLKTDAGDTVP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 766          

HSP 8 Score: 64.6994 bits (156), Expect = 1.544e-9
Identity = 87/350 (24.86%), Postives = 167/350 (47.71%), Query Frame = 1
Query: 1981 PLLTSKDKVYPQSVASR------SSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIK----TLVNDVKFQRGILIAILINTLSMGIE---YHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGI-LG---YIKNGFNLFDGIIVVLGVIELC--QQGGTS---GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CT---IEN--NKRTC---------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQ 2886
            PLL+   +VYPQ  A +        +  S  ++I ++ + Y    RP +  + +     YL   Q K +     ++    F   +L  I +N +++ +E    HQ  ++    L  SN +F+ +F+ EM +K++  G+ LG   Y+++ +N+ DG +V + +I++     GG      L +LR  R LR L+++   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF  C    ++N  NK  C          + NFD+L  +++++F + +++ W  ++Y+G+               W  LYFI+ +   ++ + N+ V ++VE F   R  ++
Sbjct: 1012 PLLS---EVYPQLNARKDRPHLDEETDYSLCFRIQKMMEVY----RP-DWCETRENWSVYLFSPQNKFRLTCQKIIAHKLFDYVVLAFIFLNCITVALERPKIHQGSLERV-FLTISNYIFTAIFVAEMTVKVVSMGLYLGERAYLRSSWNILDGFLVFVSLIDIVVSMAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCVGLDVKNITNKSDCLSAKYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVGIDQQPVINNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQE 1352          

HSP 9 Score: 61.6178 bits (148), Expect = 1.050e-8
Identity = 39/117 (33.33%), Postives = 63/117 (53.85%), Query Frame = 1
Query:  331 TVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNN 678
            +V+    QNK  R  C KII    F+ + +  I +NCIT+++ +P   Q   + +   +   +YI  A F  EM +K+++MG +LG+  Y   +WN LD F+V V  I+I+ S    
Sbjct: 1061 SVYLFSPQNKF-RLTCQKIIAHKLFDYVVLAFIFLNCITVALERPKIHQG--SLERVFLTISNYIFTAIFVAEMTVKVVSMGLYLGERAYLRSSWNILDGFLVFVSLIDIVVSMAGG 1174          

HSP 10 Score: 58.9214 bits (141), Expect = 7.200e-8
Identity = 37/129 (28.68%), Postives = 71/129 (55.04%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPV-------QLTQTLEFSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            F+R  ++ I++N +++G+    QP        Q  Q L+  + +  I F +EM++K++G GI G   Y+ + +N  D  IV+ GV+E          + +RT R+LR LK +  +P++R  + +++ T+
Sbjct:   92 FERVSMMVIILNCVTLGM---YQPCENIDCSSQRCQILQAFDAMIYIFFAVEMVVKMVGLGIFGRRCYLGDTWNRLDFFIVMAGVVEYSLDLQNINFTAIRTVRVLRPLKAINRVPSMRILVNLLLDTL 217          

HSP 11 Score: 54.6842 bits (130), Expect = 1.460e-6
Identity = 27/95 (28.42%), Postives = 51/95 (53.68%), Query Frame = 1
Query: 1150 TDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSF----------WDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            +DC   +        NFDN+G A +++F + S + WV+IMY+  DA             W  +YF++ ++I SFF++N+ + V+   F + ++ +
Sbjct: 1257 SDCLSAKYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVGIDQQPVINNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQ 1351          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: ENSAMXT00000033959.1 (pep primary_assembly:Astyanax_mexicanus-2.0:4:2732409:2930308:1 gene:ENSAMXG00000029856.1 transcript:ENSAMXT00000033959.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 292.352 bits (747), Expect = 2.616e-87
Identity = 191/368 (51.90%), Postives = 247/368 (67.12%), Query Frame = 1
Query:  244 KSKADHCQQET--DGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIIT--WPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHP-GLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIII 1332
                +H +QE    G     +E+L Y      VF    Q   PR WCLK++   +P+     M +I +NC+TL M+ PC D +C++ +C+I++ FD  IFAFF  EMVIK++A+G  G   Y GDTWNRLD FIV+ G +E   +   N+  +A+RT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGI+GVQ+W G+LRNRC+L   E+F  P  L+L ++Y        N  +  FICS  + NG+ +CS IP   MLL           A   TDN     +CVNWNQYYT+C  GE NP++GAINFDNIG+AW+AIFQVI++E WVDIMY+V DAHSF+++IYF+ LII+
Sbjct:   34 SGGGEHAEQEGAEQGAERRESEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPYPFIPNPDMLVILLNCVTLGMFHPCEDSDCDSERCKILEDFDDFIFAFFAIEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNL-QNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLP--ENFSLPQTLDLHTYYHTE-----NDDESPFICSQPRENGMRQCSSIP---MLLEETVQCHLDMGAYNTTDN----TTCVNWNQYYTNCSAGEVNPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIV 386          

HSP 2 Score: 69.707 bits (169), Expect = 1.208e-11
Identity = 55/156 (35.26%), Postives = 85/156 (54.49%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M  P       S+    L   +      F  EM+IK+++ G+  G + YL   WN +D F+V   +L+  + +     N  F  +R    +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   96 MLVILLNCVTLGMFHPCEDSDCDSERCKILEDFDDFIFAFFAIEMVIKMVALGIF-GKKCYLGDTWNRLDFFIVLAGMLEYSLNL----QNVSFSAVRT---VRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAG 243          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Cavefish
Match: ENSAMXT00000056794.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:27265512:27410284:-1 gene:ENSAMXG00000029376.1 transcript:ENSAMXT00000056794.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 277.715 bits (709), Expect = 1.865e-82
Identity = 173/334 (51.80%), Postives = 226/334 (67.66%), Query Frame = 1
Query:  331 TVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIII 1332
             VF    Q   PR+WC+++       ++S+ +I +NC+TL MYQPC + +C + +CQI+QAFD  I+ FF  EMV+K++A+G  G   Y GDTWNRLD FIV+ G +E       NI L+AIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNR    ++ S   P +    +YQ          ++ FICS  ++NGI+ C+D+P           + C      F   +     CVNWNQYYT C  G  NP++GAINFDNIG+AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LII+
Sbjct:   57 VVFFCLKQTTCPRNWCIRM-------RISIMVILLNCVTLGMYQPCENIDCTSDRCQILQAFDAFIYIFFALEMVVKMVALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDL-QNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRFQTKIDPSGISPPV---PYYQPEED-----DERPFICSLAQDNGIMSCTDVPTR----REGGTECCLDKEDVFVSAM---GLCVNWNQYYTRCFTGNSNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFIFLIIV 367          

HSP 2 Score: 72.4034 bits (176), Expect = 1.513e-12
Identity = 55/156 (35.26%), Postives = 89/156 (57.05%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M +P  +I   S     L   +    I F  EM++K+++ G+  G + YL   WN +D F+V   +++   ++ L + N     L   R +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   79 IMVILLNCVTLGMYQPCENIDCTSDRCQILQAFDAFIYIFFALEMVVKMVALGIF-GRRCYLGDTWNRLDFFIVMAGMVEY--SLDLQNIN-----LSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 226          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001901.1 (pep scaffold:Pmarinus_7.0:GL477793:3961:23506:1 gene:ENSPMAG00000001722.1 transcript:ENSPMAT00000001901.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 305.834 bits (782), Expect = 1.734e-93
Identity = 163/267 (61.05%), Postives = 210/267 (78.65%), Query Frame = 1
Query: 3796 QPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQEN 4596
            +PK   +++ + SLY+F+  N+ R +C+ I+  K FD++VL FI LNCIT+A+ERP I   S ERLF+T++NYIFT +F  EM IKV++  L+ GD AYL+S WN +DGFLV +SL+D+ +++      RI GILRV RLLRTLRPLRVISRAPGLKLVV+TL++SL+PIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  K+DCL+ +  +W   KYNFDNLGQALM+LFVLSSKDGWV IMYTG+DAVG++ Q  ++
Sbjct:    3 KPKFCESRKEW-SLYLFAPHNKVRMQCRKIIAHKVFDHVVLLFIFLNCITIALERPDIGADSPERLFMTISNYIFTAVFVTEMSIKVIAMALVFGDGAYLRSTWNTLDGFLVLVSLVDIIVSLASDSGARILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLDTKNITNKSDCLR-ANYKWLKHKYNFDNLGQALMSLFVLSSKDGWVNIMYTGLDAVGLDQQRSKD 267          

HSP 2 Score: 70.4774 bits (171), Expect = 8.977e-13
Identity = 40/117 (34.19%), Postives = 64/117 (54.70%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F  +   R  C KII    F+ + +  I +NCIT+++ +P  D   ++ +   M   +YI  A F  EM IK+IAM  + GD  Y   TWN LD F+V+V  ++I+ S  ++ G
Sbjct:   15 LYLFAPHNKVRMQCRKIIAHKVFDHVVLLFIFLNCITIALERP--DIGADSPERLFMTISNYIFTAVFVTEMSIKVIAMALVFGDGAYLRSTWNTLDGFLVLVSLVDIIVSLASDSG 129          

HSP 3 Score: 50.0618 bits (118), Expect = 3.375e-6
Identity = 73/249 (29.32%), Postives = 126/249 (50.60%), Query Frame = 1
Query: 4777 HLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTA-----LKVFNYFFTSTFVXXXXXXXXXXXXX----RYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELF-GRLE-CSDINP--------CV-----GLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            +L AP     ++K R     ++  K FD  +   I LN IT+A E    P +   +     + + NY FT+ FV E  +K++A+ L      Y+   WN LD  +V++S+  +++     +   I   I+RV+R+LR  R L+++  A G++ +++T+I +L  +GN+ L+    F IF  LGV+LF G+   C  ++         C+      L    +F+N G A ++LF +++ D W  IM   L
Sbjct:   16 YLFAP-----HNKVRMQCRKIIAHKVFDHVVLLFIFLNCITIALE---RPDIGADSPERLFMTISNYIFTAVFVTEMSIKVIAMALVFGDGAYLRSTWNTLDGFLVLVSLVDIIVSLASDSGARIL-GILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLDTKNITNKSDCLRANYKWLKHKYNFDNLGQALMSLFVLSSKDGWVNIMYTGL 255          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001900.1 (pep scaffold:Pmarinus_7.0:GL477793:3961:23576:1 gene:ENSPMAG00000001722.1 transcript:ENSPMAT00000001900.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 306.99 bits (785), Expect = 2.109e-93
Identity = 170/303 (56.11%), Postives = 227/303 (74.92%), Query Frame = 1
Query: 3796 QPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQ---ENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            +PK   +++ + SLY+F+  N+ R +C+ I+  K FD++VL FI LNCIT+A+ERP I   S ERLF+T++NYIFT +F  EM IKV++  L+ GD AYL+S WN +DGFLV +SL+D+ +++      RI GILRV RLLRTLRPLRVISRAPGLKLVV+TL++SL+PIGNIVLICC FFIIFGILGVQLFKGKFYYC+G   + +  K+DCL+ +  +W   KYNFDNLGQALM+LFVLSSKDGWV IMYTG+DAVG++ Q     +N N++ ++   +FL L+   ++ M +   + + H
Sbjct:    3 KPKFCESRKEW-SLYLFAPHNKVRMQCRKIIAHKVFDHVVLLFIFLNCITIALERPDIGADSPERLFMTISNYIFTAVFVTEMSIKVIAMALVFGDGAYLRSTWNTLDGFLVLVSLVDIIVSLASDSGARILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLDTKNITNKSDCLR-ANYKWLKHKYNFDNLGQALMSLFVLSSKDGWVNIMYTGLDAVGLDQQRSKDPKNSNDFFVLKCCTFLRLLLLLLMTMMITAPLHSRH 303          

HSP 2 Score: 70.8626 bits (172), Expect = 1.109e-12
Identity = 40/117 (34.19%), Postives = 64/117 (54.70%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F  +   R  C KII    F+ + +  I +NCIT+++ +P  D   ++ +   M   +YI  A F  EM IK+IAM  + GD  Y   TWN LD F+V+V  ++I+ S  ++ G
Sbjct:   15 LYLFAPHNKVRMQCRKIIAHKVFDHVVLLFIFLNCITIALERP--DIGADSPERLFMTISNYIFTAVFVTEMSIKVIAMALVFGDGAYLRSTWNTLDGFLVLVSLVDIIVSLASDSG 129          

HSP 3 Score: 50.447 bits (119), Expect = 2.964e-6
Identity = 73/249 (29.32%), Postives = 126/249 (50.60%), Query Frame = 1
Query: 4777 HLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTA-----LKVFNYFFTSTFVXXXXXXXXXXXXX----RYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELF-GRLE-CSDINP--------CV-----GLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            +L AP     ++K R     ++  K FD  +   I LN IT+A E    P +   +     + + NY FT+ FV E  +K++A+ L      Y+   WN LD  +V++S+  +++     +   I   I+RV+R+LR  R L+++  A G++ +++T+I +L  +GN+ L+    F IF  LGV+LF G+   C  ++         C+      L    +F+N G A ++LF +++ D W  IM   L
Sbjct:   16 YLFAP-----HNKVRMQCRKIIAHKVFDHVVLLFIFLNCITIALE---RPDIGADSPERLFMTISNYIFTAVFVTEMSIKVIAMALVFGDGAYLRSTWNTLDGFLVLVSLVDIIVSLASDSGARIL-GILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYYCEGLDTKNITNKSDCLRANYKWLKHKYNFDNLGQALMSLFVLSSKDGWVNIMYTGL 255          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001904.1 (pep scaffold:Pmarinus_7.0:GL477793:127370:134209:1 gene:ENSPMAG00000001732.1 transcript:ENSPMAT00000001904.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 222.631 bits (566), Expect = 1.732e-65
Identity = 159/229 (69.43%), Postives = 186/229 (81.22%), Query Frame = 1
Query: 4933 LKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVS--AIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHK 5610
            L   LK  NY FTS FVLEALLKL+A G+ RY  D+WNQLD+ IV+LSI G++++E+E S  +PINPTI+R+MRVLRI RVLKL+KMA+G++ALL  V+QALPQVGNLGLLF LLF+I+AALGVELFG L C D  PC GL RHA FENFGMA LTLFR++TGDNWNGIMKDTLRE   SS D Q   C+S   +I+PIYFV FVL AQFVLVNVVVAVLMKHLE+S +
Sbjct:   46 LNEGLKYCNYVFTSVFVLEALLKLLAFGIRRYFKDRWNQLDLAIVLLSIMGIILEEIEISASLPINPTIIRIMRVLRITRVLKLVKMATGMQALLAVVVQALPQVGNLGLLFLLLFFIYAALGVELFGGLTCEDY-PCEGLSRHATFENFGMALLTLFRVSTGDNWNGIMKDTLRE--CSSLDRQ---CISYLPVISPIYFVTFVLTAQFVLVNVVVAVLMKHLEDSAE 268          

HSP 2 Score: 77.0258 bits (188), Expect = 3.309e-15
Identity = 65/229 (28.38%), Postives = 113/229 (49.34%), Query Frame = 1
Query: 3997 LTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFIT-ILLGHSNRI-FGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG---KFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIM------YTGIDAVGVNIQPQENHNEWALIYFISFLLLVAF 4650
            L   NY+FT +F  E ++K+L+ G+    + Y K  WN +D  +V +S++ + +  I +  S  I   I+R+ R+LR  R L+++  A G++ ++  ++ +L  +GN+ L+    F I+  LGV+LF G   + Y C+G    A                     F+N G AL+ LF +S+ D W  IM       + +D   ++  P  +      IYF++F+L   F
Sbjct:   50 LKYCNYVFTSVFVLEALLKLLAFGI----RRYFKDRWNQLDLAIVLLSIMGIILEEIEISASLPINPTIIRIMRVLRITRVLKLVKMATGMQALLAVVVQALPQVGNLGLLFLLLFFIYAALGVELFGGLTCEDYPCEGLSRHA--------------------TFENFGMALLTLFRVSTGDNWNGIMKDTLRECSSLDRQCISYLPVISP-----IYFVTFVLTAQF 249          

HSP 3 Score: 68.1662 bits (165), Expect = 2.479e-12
Identity = 49/193 (25.39%), Postives = 101/193 (52.33%), Query Frame = 1
Query: 2224 NTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            ++L   +++      L + L++ N VF+ +F++E +LKL+  GI  Y K+ +N  D  IV+L ++ +  +            + + I+R  R+ R+LKLV+    ++  L V+++ +  V     L +L  FI++ LG+ LFG   C     +       F++   +++T+F++ T ++WN ++ + +   SS
Sbjct:   31 SSLPFAVKHRGVRHSLNEGLKYCNYVFTSVFVLEALLKLLAFGIRRYFKDRWNQLDLAIVLLSIMGIILEEIEISASLPINPTIIRIMRVLRITRVLKLVKMATGMQALLAVVVQALPQVGNLGLLFLLLFFIYAALGVELFGGLTCEDYPCEGLSRHATFENFGMALLTLFRVSTGDNWNGIMKDTLRECSS 223          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009098.1 (pep scaffold:Pmarinus_7.0:GL478807:14086:32911:1 gene:ENSPMAG00000008235.1 transcript:ENSPMAT00000009098.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 204.912 bits (520), Expect = 1.212e-60
Identity = 118/183 (64.48%), Postives = 147/183 (80.33%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQI 2718
            +VN   F RGI++AILINT+SM IEYH+QP +LT  LE SNIVF+ +F +EM+LK+   G+ GYI+N +N+FDG+IV++ V E+  Q G  GLS+LRTFRLLR+LKLVRFMPALR+QL V++RT+DNVATF  LL+LFIFIFSILGM LFGCKF    +   T  DRKNFDSLLW+IVTVFQ+
Sbjct:    1 IVNSKYFNRGIMVAILINTVSMAIEYHEQPEELTNILEISNIVFTSMFALEMLLKIFACGLFGYIRNQYNIFDGVIVIISVWEIVGQSG-GGLSVLRTFRLLRVLKLVRFMPALRRQL-VLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDTGDTIPDRKNFDSLLWAIVTVFQV 181          

HSP 2 Score: 72.0182 bits (175), Expect = 2.317e-14
Identity = 59/210 (28.10%), Postives = 104/210 (49.52%), Query Frame = 1
Query: 3883 IVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALF 4512
            IV+ K F+  ++  I +N +++A+E    P        L ++N +FT +F  EM++K+ + GL      Y+++ +NI DG +V IS+ +     ++G S     +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 LK    +    + NFD+L  A++ +F
Sbjct:    1 IVNSKYFNRGIMVAILINTVSMAIEYHEQPEELTN--ILEISNIVFTSMFALEMLLKIFACGLF----GYIRNQYNIFDGVIVIISVWE-----IVGQSGGGLSVLRTFRLLRVLKLVRFM---PALRRQL-VLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------SLKTDTGDTIPDRKNFDSLLWAIVTVF 179          

HSP 3 Score: 57.3806 bits (137), Expect = 3.128e-9
Identity = 46/123 (37.40%), Postives = 69/123 (56.10%), Query Frame = 1
Query: 4837 VVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIR 5205
            +V SKYF+  I   I +N ++MA E+H  P+ L   L++ N  FTS F LE LLK+ A GL  YI +++N  D +IVI+S+  +V               + V+R  R+ RVLKL++    +R
Sbjct:    1 IVNSKYFNRGIMVAILINTVSMAIEYHEQPEELTNILEISNIVFTSMFALEMLLKIFACGLFGYIRNQYNIFDGVIVIISVWEIVGQSGGG---------LSVLRTFRLLRVLKLVRFMPALR 114          

HSP 4 Score: 51.2174 bits (121), Expect = 3.699e-7
Identity = 38/123 (30.89%), Postives = 65/123 (52.85%), Query Frame = 1
Query:  385 IITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMR 753
            I+   +F +  M  I +N  T+SM     +Q  E +   I++  + +  + F  EM++KI A G  G   Y  + +N  D  IVI+   EI+  +G   GL+ +RT R+LR L+ +  +P++R
Sbjct:    1 IVNSKYFNRGIMVAILIN--TVSMAIEYHEQPEELT--NILEISNIVFTSMFALEMLLKIFACGLFG---YIRNQYNIFDGVIVIISVWEIVGQSGG--GLSVLRTFRLLRVLKLVRFMPALR 114          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008004.1 (pep scaffold:Pmarinus_7.0:GL477228:88466:118438:1 gene:ENSPMAG00000007222.1 transcript:ENSPMAT00000008004.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 207.608 bits (527), Expect = 8.154e-54
Identity = 166/569 (29.17%), Postives = 284/569 (49.91%), Query Frame = 1
Query: 3781 SKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYC---DGPHLQAVKTKN----DCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAP--LYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREK 5460
            S CC    G F     H++         R+    +     F+ LVLF + ++  TL ++      +++    L   +  +   F+ EM +K++  GL      Y  S W ++D FLV +S L+      L  +      LR  R LR LRPLR ISR  G++LVV  L++S+  I N++L+C  F+++FGI GVQLF GKF+ C   +G  L A +  N    + L      W N   N+DN+  A +AL  +++ +GW++ M    D+ GV+ QP  N N ++ ++FI F+++ +FF+LN+F+GVV++NF+   + +          ++      +R +   P     +  ++  R    +V  + F+     VI LN++ +A+E +   +      K+ N  FT  F+       +      Y    W+  D  +V +S+  +V++ + S  +  +P I+RV RV+R+ R+L+L++   GIR L+ T++ +LP + N+GLL  L+ +++  LGV +F  L  S       +    +FE F  +   LFR+ T   WN +M   L  K
Sbjct:  997 SGCCGGCVGAF-----HAV---------RRMVFRLCHTTGFNGLVLFMVIMSSFTLTLDDKYNSSNAQLMQVLRYMDIFYMAFFSMEMFLKMIGLGL----HKYFTSFWTLLDCFLVLVSWLNQ-----LASAVPALKSLRSLRTLRALRPLRAISRWKGMRLVVNALVTSIPSISNVMLMCLLFWMVFGIAGVQLFGGKFFRCIDAEGNRLPATEVANRTVCESLAAEGYLWVNAIINYDNILSAYLALLQVATLEGWIEQMADASDSTGVDQQPSYNANIYSQMFFIIFVIIGSFFILNLFIGVVIDNFNSVHKREGALMALLTEDQQKFYKTMRRLYRSKPSKSIPQPQNRVLRFFYLLVHRRLFEYISTAVISLNMVMLASEHYKQSESFTNITKIINLVFTGLFM-LEAALKILGNRIHYFRQPWDIFDFFVVCISLTEIVLETMASQ-VNFSPAILRVFRVVRLVRLLRLIRSVEGIRKLIYTLVISLPALINIGLLLLLIMFMYGVLGVSIFRDLPYSG-----SITNMVNFETFANSMTLLFRLNTAGGWNDVMGSVLGTK 1535          

HSP 2 Score: 76.2554 bits (186), Expect = 7.269e-14
Identity = 64/204 (31.37%), Postives = 103/204 (50.49%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTS---GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN--NKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLY 2751
            +Q+++TLV    F+  I I I+ NT+ M I+Y+  P   +  L+  N V + +F+ E +LKL       Y   G+N+FD  +V+  V+E      ++    +SI+RTFR+LR+ KL +    L   + VM   I  +     +  L I+IF+++GM L G  +    N  +     R NF     S + VF++L  E W   LY
Sbjct:  558 RQRLRTLVTSSVFETFITILIITNTVMMAIDYYNAP--YSSALDIGNTVLTTIFIAEAVLKLTALFPRVYFHLGWNIFDFCVVITSVVEWILDSMSNTNINISIIRTFRILRVFKLAKKWKTLNLLIRVMKSAIGEMRNLTVIFALVIYIFAVIGMQLIGRDYYHNRNKFSSGQVPRWNFLDFYSSFLMVFRVLCGE-WIEPLY 758          

HSP 3 Score: 75.8702 bits (185), Expect = 1.001e-13
Identity = 80/312 (25.64%), Postives = 148/312 (47.44%), Query Frame = 1
Query: 3781 SKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQA-YLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            SK  PQP+    + +Y                  +V ++ F+Y+    I+LN + LA E      H K+    T    I  ++FTG  +++   K  ++G++  Y +  W+I D F+V ISL ++ +  +    N    ILRVFR++R +R LR+I    G++ ++ TL+ SL  + NI L+      ++G+LGV +F+      D P+  ++                   NF+    ++  LF L++  GW  +M       G  +  +  +    + YF +F++     ++NM+V +++ENF++ QE +
Sbjct: 1318 SKSIPQPQNRVLRFFYL-----------------LVHRRLFEYISTAVISLNMVMLASE------HYKQSESFTNITKIINLVFTGLFMLEAALK--ILGNRIHYFRQPWDIFDFFVVCISLTEIVLETMASQVNFSPAILRVFRVVRLVRLLRLIRSVEGIRKLIYTLVISLPALINIGLLLLLIMFMYGVLGVSIFR------DLPYSGSI---------------TNMVNFETFANSMTLLFRLNTAGGWNDVM-------GSVLGTKTQNAILVIAYFTTFVIFSNIIIINMYVAIILENFNEAQEQE 1576          

HSP 4 Score: 75.8702 bits (185), Expect = 1.027e-13
Identity = 41/122 (33.61%), Postives = 65/122 (53.28%), Query Frame = 1
Query: 4012 YIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            YIFT I+TGE +IK L++G +    +YL++ WN +D           FI I+L +    F  L     LR  R L++I+  P LK +V  +  S   + N++L+     II  + G+Q+F+G
Sbjct:    1 YIFTAIYTGEFLIKTLARGFVFHPYSYLRNAWNWLD-----------FIVIILAYMTIAFPFLPGLSALRAFRALKMIAILPALKTIVGAIFKSAGLLLNVMLLMIFALIIISLFGLQIFRG 111          

HSP 5 Score: 70.4774 bits (171), Expect = 4.851e-12
Identity = 65/279 (23.30%), Postives = 128/279 (45.88%), Query Frame = 1
Query: 2173 LVNDVKFQRGILIAILIN--TLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG--GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF------------CTIENNKRTCDRK------------NFDSLLWSIVTVFQILTQEDWNTVLYN-----GMTRTSSWAA-----LYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLL 2895
            L +   F   +L  ++++  TL++  +Y+    QL Q L + +I +   F +EM LK+IG G+  Y  + + L D  +V++  +            L  LRT R LR L+ +     +R  +  ++ +I +++    + +LF  +F I G+ LFG KF             T   N+  C+              N+D++L + + + Q+ T E W   + +     G+ +  S+ A     ++FI  +  G++ + NL + ++++ F+S    E  L+
Sbjct: 1017 LCHTTGFNGLVLFMVIMSSFTLTLDDKYNSSNAQLMQVLRYMDIFYMAFFSMEMFLKMIGLGLHKYFTSFWTLLDCFLVLVSWLNQLASAVPALKSLRSLRTLRALRPLRAISRWKGMRLVVNALVTSIPSISNVMLMCLLFWMVFGIAGVQLFGGKFFRCIDAEGNRLPATEVANRTVCESLAAEGYLWVNAIINYDNILSAYLALLQVATLEGWIEQMADASDSTGVDQQPSYNANIYSQMFFIIFVIIGSFFILNLFIGVVIDNFNSVHKREGALM 1295          

HSP 6 Score: 64.3142 bits (155), Expect = 3.182e-10
Identity = 44/176 (25.00%), Postives = 89/176 (50.57%), Query Frame = 1
Query: 3862 FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYY 4389
            FRQ+ + +V    F+  +   I  N + +A++  + P  S     L + N + T IF  E ++K+ +    +  + Y   GWNI D  +V  S+++ +I   + ++N    I+R FR+LR  +   +  +   L L+++ + S++  + N+ +I      IF ++G+QL    +Y+
Sbjct:  557 FRQRLRTLVTSSVFETFITILIITNTVMMAIDYYNAPYSSA----LDIGNTVLTTIFIAEAVLKLTA----LFPRVYFHLGWNIFDFCVVITSVVE-WILDSMSNTNINISIIRTFRILRVFK---LAKKWKTLNLLIRVMKSAIGEMRNLTVIFALVIYIFAVIGMQLIGRDYYH 720          

HSP 7 Score: 63.929 bits (154), Expect = 4.200e-10
Identity = 76/280 (27.14%), Postives = 129/280 (46.07%), Query Frame = 1
Query:  520 YIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEI----LPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFF 1344
            YI  A +T E +IK +A GF+     Y  + WN LD  ++I+  + I    LP      GL+A+R     R L+ I  +P+++ +V  +  +  +L NV+LL  F   I  + G+Q+++GVLR +C    N     PG +  S             D ++       +  L   DI   IM+  N +           T + P  ++C+           GE NP  GA +FDN G+A +  FQ+I+++ W +    V  A   +   +F+ +I++G ++
Sbjct:    1 YIFTAIYTGEFLIKTLARGFVFHPYSYLRNAWNWLDFIVIILAYMTIAFPFLP------GLSALRA---FRALKMIAILPALKTIVGAIFKSAGLLLNVMLLMIFALIIISLFGLQIFRGVLRQKCVFLPN-----PGTSSLS-------------DAEWSNYVQNKSNWLITDDI--EIMVCGNGT----------GTSHCPANYTCLP--------DIGE-NPNHGATSFDNFGWAMMTSFQIITIDYWENCYNMVLRASGQYFIGFFIVVIMLGGYY 232          

HSP 8 Score: 63.929 bits (154), Expect = 4.608e-10
Identity = 44/122 (36.07%), Postives = 67/122 (54.92%), Query Frame = 1
Query:  403 FEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYI-IF--AFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNI-GLTAIRTIRVLRPLRAINRIPSMRI 756
            F  L + ++ ++  TL++     D    +S  Q+MQ   Y+ IF  AFF+ EM +K+I +G      YF   W  LD F+V+V  +  L S    +  L ++RT+R LRPLRAI+R   MR+
Sbjct: 1023 FNGLVLFMVIMSSFTLTL-----DDKYNSSNAQLMQVLRYMDIFYMAFFSMEMFLKMIGLGLHK---YFTSFWTLLDCFLVLVSWLNQLASAVPALKSLRSLRTLRALRPLRAISRWKGMRL 1136          

HSP 9 Score: 58.151 bits (139), Expect = 2.288e-8
Identity = 68/277 (24.55%), Postives = 140/277 (50.54%), Query Frame = 1
Query: 2086 SKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSI-------LRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAAL---YFIALMTFGNYVLFNLLVAILVEGFSSERLDEQ 2886
            SK  +   + QN+  R  YL         LV+   F+      I +N + +  E+++Q    T   +  N+VF+ LF++E  LK++G  I  Y +  +++FD  +V + + E+  +   S ++         R  RL+R+L+L+R +  +R+ ++ ++ ++  +     LL+L +F++ +LG+S+F      +  +    +  NF++   S+  +F++ T   WN V+ + +   +  A L   YF   + F N ++ N+ VAI++E F+  +  E+
Sbjct: 1315 SKPSKSIPQPQNRVLRFFYL---------LVHRRLFEYISTAVISLNMVMLASEHYKQSESFTNITKIINLVFTGLFMLEAALKILGNRI-HYFRQPWDIFDFFVVCISLTEIVLETMASQVNFSPAILRVFRVVRLVRLLRLIRSVEGIRKLIYTLVISLPALINIGLLLLLIMFMYGVLGVSIFR----DLPYSGSITNMVNFETFANSMTLLFRLNTAGGWNDVMGSVLGTKTQNAILVIAYFTTFVIFSNIIIINMYVAIILENFNEAQEQEE 1577          

HSP 10 Score: 53.1434 bits (126), Expect = 7.971e-7
Identity = 27/85 (31.76%), Postives = 48/85 (56.47%), Query Frame = 1
Query: 1192 INFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERM 1416
            IN+DNI  A++A+ QV ++E W++ M    D+           + +  ++F+  +IIGSFF++NL + V+   F+   +RE   M
Sbjct: 1211 INYDNILSAYLALLQVATLEGWIEQMADASDSTGVDQQPSYNANIYSQMFFIIFVIIGSFFILNLFIGVVIDNFNSVHKREGALM 1295          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Yeast
Match: CCH1 (Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function [Source:SGD;Acc:S000003449])

HSP 1 Score: 78.1814 bits (191), Expect = 1.443e-14
Identity = 77/331 (23.26%), Postives = 138/331 (41.69%), Query Frame = 1
Query: 3829 HSLYVFSVDNQFRQKCQHIV---------------------DKKSFDYLV-----LFFITLNCITLAMERPSIPPHSKERLFLTVTNY------IFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQ-TLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQN---EWK-------NQKY-NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVEN 4689
            +S Y FS  ++FR+ CQ +V                     +K+S+ + +     +F   L  I L +    + P  +    +   N+       F   F+ E I+K ++ G +    AYL++ WN +D F V IS+    I  L  + N    + R+F+ L  LR LR ++ +   +      +   L  I    LI  +    F + G+ +FKG+   C+   L     + DC     N   +W         Q Y + D+   A  +L+ + S +GWV ++   +++ G+        +    ++ + F  L   F+LN+FV  +V N
Sbjct: 1154 YSYYFFSPQHRFRRFCQRLVPPSTGKRTDGSRFFEDSTDLYNKRSYFHHIERDVFVFIFALATILLIVCSCYVTPLYRMHHKMGTWNWSSALDCAFIGAFSIEFIVKTVADGFIYSPNAYLRNPWNFID-FCVLISMWINLIAYLKNNGN----LSRIFKGLTALRALRCLTISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGRLGTCNDGSL----GRADCYNEYSNSVFQWDIMSPRVYQQPYLHLDSFASAFSSLYQIISLEGWVDLLENMMNSSGIGTPATVMGSAGNALFLVLFNFLSMVFILNLFVSFIVNN 1475          

HSP 2 Score: 63.1586 bits (152), Expect = 5.221e-10
Identity = 58/248 (23.39%), Postives = 108/248 (43.55%), Query Frame = 1
Query: 4084 QAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF--------------------YYCDGPHLQAVKTKND----------------CLKYSQNEWKNQKYN----FDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQE 4707
            +A+ +S WN +D        L +F++I    S      +R+F+ L  LR LR+++   G+  +++ L   +  + N+  +   F+I FGILGVQ+F+G F                     +C G      K K +                C +YS+       YN    FDN+  ++  +FV+ S + +  +MY  +D+  +           A ++FI  + ++  ++LN+ + V+V +F    E
Sbjct:  487 RAFARSSWNRIDLVSSVSFWLGMFLSI---KSYDTKTGIRIFKPLAILRILRLVNVDTGMPSILRGLKYGIPQLVNVSSMLVYFWIFFGILGVQIFQGSFRRQCVWFNPEDPTDTYQYDMQFCGGYLDPVTKRKQNYIYEDGSEGSVSKGFLCPQYSKCVSNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYTMDSDEMA----------ACLFFIVCIFVLTIWLLNLLIAVLVSSFEIANE 721          

HSP 3 Score: 54.299 bits (129), Expect = 2.652e-7
Identity = 27/96 (28.12%), Postives = 50/96 (52.08%), Query Frame = 1
Query: 1147 YTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            Y+ C     NPY G I+FDNI  +   +F ++S  ++ D+MYY  D+      ++F+  I + + +++NL + V+ S F        ++ +I   R
Sbjct:  639 YSKCV-SNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYTMDSDEMAACLFFIVCIFVLTIWLLNLLIAVLVSSFEIANEEYKKKKFIYGSR 733          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO36844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHI0])

HSP 1 Score: 592.423 bits (1526), Expect = 2.212e-178
Identity = 371/720 (51.53%), Postives = 479/720 (66.53%), Query Frame = 1
Query: 3799 PKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDIN-PCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRN----CCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKN-------LSRSHPHL 5922
            P    +KR   SLYVF+ DN+FR   + +   K FD  VL FI LNC+ +A+E PS+ P S ER  + +  Y+F  IFT EM++KV++ GL IG  AYL+SGWN+MDGFLV IS +D+ +T      N I G+LRVFR LRTLRPLRVISRAPGLK+VV+TL+SSL+PIGNIVLI  TFFIIFGILGVQLFKGKF+YC    +  V TK +CL+ +   W N++YNFDNL +AL+ LFV S+KDGWV IMY GIDAVG++ QP  NH  + +IYF+ FLLL  F VLNM VGVVVENF KC+   E E+K     K  +  + +R+        E+Y + R+ I++V T  YFDL IA VI LNV+ MA E +  P  L   LK  NY FT+ F+LEA+LK+ ALG+ RYI D+WNQLDM+IVILSI G+ ++E+    +PINPTI+RVMRVLRIARVLKLLK A GIR LLDTV QALPQVGNLG+LF L+F+IF+ALG+ELFG+++C      C G+  HA+F +FG+A LTLFRI+TGDNWNGI+KDT     IS + C+ N    C +   +APIYF +FVL  QFVL+NVVVAVLMKHLEE+           ++ +  ++      +  +  KD  NN  +L     D+  +F D+ +N S  +++  +  C    ++N Q  N       L  I  ++       LSRS P L
Sbjct:  899 PTSCCSKRADWSLYVFAPDNRFRMLNKELYQNKWFDRTVLLFILLNCVVMALEGPSVLPGSLERRVIDICMYVFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGKFHYCTDATV-PVTTKTECLE-NGGSWVNREYNFDNLAKALLTLFVFSTKDGWVNIMYDGIDAVGIDKQPIRNHARYNVIYFVGFLLLAGFVVLNMLVGVVVENFQKCRTLIEMEKKIDEEKKNKKEEKMRREIAEDEALSEHYPRPRKFIHAVCTHGYFDLGIAAVIALNVLCMALEHYQQPDGLTAFLKCANYVFTAVFILEAILKIFALGIKRYIKDRWNQLDMLIVILSIVGIALEEMTME-LPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELFGKIDCEKPGITCQGMDEHANFRSFGIAMLTLFRISTGDNWNGILKDT-----ISPDVCRVNPEIDCSMLEHVAPIYFAVFVLATQFVLLNVVVAVLMKHLEEA-----------KDTKTPVNTPPLSSSSRESSKD--NNGASLKVPSSDRKVSFDDEADNYSKPESRSSR--CLPMVAVNGQGYNSFDNEFGLQGITRQDAIKPPAMLSRSAPSL 1595          

HSP 2 Score: 258.455 bits (659), Expect = 4.076e-69
Identity = 139/228 (60.96%), Postives = 175/228 (76.75%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCD--RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS 2868
            F   I+ AI +NTL+MGIEY+ QP ++T  LE  N +F+ +F IEMI+KLIG G  GYIK+ FN+FDG IV++ V+EL      SG+S+LR+FRLLR+ KLVRF+PALR+QL VMI T+DNV TF ALL++F+F  SILGM+LFG K+    N++   +  R NFD L W+IVTVFQ+LTQEDWN V+Y+GM  TS WA LYFI LMT GNY+LFNLLVAILVEGF+S
Sbjct:  413 FMYVIMGAIFVNTLTMGIEYYGQPQKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGDD-DSGISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKY-RFPNDEGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMRATSKWAGLYFILLMTIGNYILFNLLVAILVEGFAS 638          

HSP 3 Score: 211.846 bits (538), Expect = 7.409e-55
Identity = 133/250 (53.20%), Postives = 169/250 (67.60%), Query Frame = 1
Query:  685 LTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRR 1434
            +TA+R IRVLRPLRAINR+PS+RILV LLLDTLPMLGNVL +C  +F IFGI+GVQMWQGVLR+RC   L  +     LN+S+FYQ   +        D +CS  +N+G   C+    + +  S    + C      +++     + C NWNQYYT C     NP   A++FDNI  AWVAIFQVI++E W DIMYY+QDAH FW++IYFV LI+IGS+FM NLCLVVI +QF ETK+RE + M   RR+
Sbjct:    1 MTAVRAIRVLRPLRAINRVPSIRILVTLLLDTLPMLGNVLAMCSLIFSIFGIVGVQMWQGVLRSRCVAQLPGNLSANQLNISAFYQPGGS-------SDLVCSLPENSGDKHCTS---AFIPASTAHGRECGLTYEQYSNRSNYTNVCANWNQYYTLCADMGINPLYDAVSFDNILIAWVAIFQVITLEGWTDIMYYIQDAHGFWNFIYFVVLIVIGSYFMTNLCLVVITTQFQETKQRENDLMRQSRRK 240          

HSP 4 Score: 119.783 bits (299), Expect = 8.184e-27
Identity = 80/278 (28.78%), Postives = 138/278 (49.64%), Query Frame = 1
Query: 3859 QFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            +FR  C   VD K F Y+++  I +N +T+ +E    P   K    L + NYIFT IF  EMI+K++  G       Y+K  +NI DG +V IS+++LF     G  +    +LR FRLLR  + +R +   P L+  +  ++ ++  +   + +   F     ILG+ LF GK+ +            ND     +   +  + NFD+L  A++ +F + +++ W  +MY G+ A           ++WA +YFI  + +  + + N+ V ++VE F
Sbjct:  399 KFRDGCASSVDSKWFMYVIMGAIFVNTLTMGIEYYGQP--QKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFY----GYIKDAFNIFDGTIVIISVVELF-----GDDDSGISVLRSFRLLRVFKLVRFL---PALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKYRF-----------PND-----EGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMRAT----------SKWAGLYFILLMTIGNYILFNLLVAILVEGF 636          

HSP 5 Score: 98.5969 bits (244), Expect = 2.758e-20
Identity = 80/277 (28.88%), Postives = 139/277 (50.18%), Query Frame = 1
Query: 2167 KTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNI-----VFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSG----LSILRTFRLLRILKLVRFM---PALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CT------------IENNKRTCDRK-NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT----------SSWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            K L  +  F R +L+ IL+N + M +E    P  L  +LE   I     VF  +F IEM++K+I  G+      Y+++G+N+ DG +VV+  +++     T+G    L +LR FR LR L+ +R +   P L+  +  +I ++  +     +   F  IF ILG+ LF  KF  CT            +EN     +R+ NFD+L  +++T+F   T++ W  ++Y+G+             + +  +YF+  +    +V+ N+LV ++VE F   R
Sbjct:  925 KELYQNKWFDRTVLLFILLNCVVMALE---GPSVLPGSLERRVIDICMYVFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGKFHYCTDATVPVTTKTECLENGGSWVNREYNFDNLAKALLTLFVFSTKDGWVNIMYDGIDAVGIDKQPIRNHARYNVIYFVGFLLLAGFVVLNMLVGVVVENFQKCR 1198          

HSP 6 Score: 97.0561 bits (240), Expect = 8.036e-20
Identity = 69/197 (35.03%), Postives = 109/197 (55.33%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSI-------LRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC----DRKNFDSLLWSIVTVFQILTQEDWNTVL 2748
            F  GI   I +N L M +E++QQP  LT  L+ +N VF+ +F++E ILK+   GI  YIK+ +N  D +IV+L ++ +  +  T  L I       +R  R+ R+LKL++    +R+ L  + + +  V     L +L  FIFS LG+ LFG   C  E    TC    +  NF S   +++T+F+I T ++WN +L
Sbjct: 1249 FDLGIAAVIALNVLCMALEHYQQPDGLTAFLKCANYVFTAVFILEAILKIFALGIKRYIKDRWNQLDMLIVILSIVGIALEEMTMELPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELFGKIDC--EKPGITCQGMDEHANFRSFGIAMLTLFRISTGDNWNGIL 1443          

HSP 7 Score: 85.8853 bits (211), Expect = 1.923e-16
Identity = 67/215 (31.16%), Postives = 111/215 (51.63%), Query Frame = 1
Query: 4810 SKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLR 5454
            +K+R    S V SK+F   I G I +N +TM  E++  PQ +   L++FNY FT+ F +E ++KL+ LG   YI D +N  D  IVI+S+  +  D+            + V+R  R+ RV KL++    +R  L  +I  +  V     L  +  +  + LG+ LFG       +  V     A+F++   A +T+F++ T ++WN +M D +R
Sbjct:  398 AKFRDGCASSVDSKWFMYVIMGAIFVNTLTMGIEYYGQPQKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGDDDSG---------ISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKYRFPNDEGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMR 603          

HSP 8 Score: 70.4774 bits (171), Expect = 9.036e-12
Identity = 36/126 (28.57%), Postives = 70/126 (55.56%), Query Frame = 1
Query:  400 WFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNN-----IG-LTAIRTIRVLRPLRAINRIPSMRI 756
            WF++  +  I +NC+ +++  P       + + +++    Y+    FT EM++K+IA+G ++G   Y    WN +D F+V++  ++++ +   N     +G L   R +R LRPLR I+R P ++I
Sbjct:  932 WFDRTVLLFILLNCVVMALEGPSVLPG--SLERRVIDICMYVFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKI 1055          

HSP 9 Score: 65.855 bits (159), Expect = 2.068e-10
Identity = 38/129 (29.46%), Postives = 67/129 (51.94%), Query Frame = 1
Query:  367 RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMR 753
            RD C   +   WF  + M  I VN +T+ +      Q        +++ F+YI  A F  EM++K+I +GF    GY  D +N  D  IVI+  +E+     ++ G++ +R+ R+LR  + +  +P++R
Sbjct:  401 RDGCASSVDSKWFMYVIMGAIFVNTLTMGIEYYGQPQK----MTDVLEIFNYIFTAIFGIEMIMKLIGLGF---YGYIKDAFNIFDGTIVIISVVELF--GDDDSGISVLRSFRLLRVFKLVRFLPALR 520          

HSP 10 Score: 62.003 bits (149), Expect = 3.185e-9
Identity = 26/60 (43.33%), Postives = 42/60 (70.00%), Query Frame = 1
Query: 4198 LRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +   R +R LRPLR I+R P ++++V  LL +L  +GN++ +C   F IFGI+GVQ+++G
Sbjct:    1 MTAVRAIRVLRPLRAINRVPSIRILVTLLLDTLPMLGNVLAMCSLIFSIFGIVGVQMWQG 60          

HSP 11 Score: 54.6842 bits (130), Expect = 5.579e-7
Identity = 23/66 (34.85%), Postives = 40/66 (60.61%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSET 1392
            NFD++ +A V +FQV++ E W  +MY    A S W  +YF+ L+ IG++ + NL + ++   F+  
Sbjct:  574 NFDDLFWAIVTVFQVLTQEDWNIVMYDGMRATSKWAGLYFILLMTIGNYILFNLLVAILVEGFASA 639          

HSP 12 Score: 53.5286 bits (127), Expect = 1.251e-6
Identity = 26/86 (30.23%), Postives = 46/86 (53.49%), Query Frame = 1
Query: 2665 DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKK 2922
            D  +FD++L + V +FQ++T E W  ++Y        W  +YF+ L+  G+Y + NL + ++   F   +  E  L+   RQS +K
Sbjct:  158 DAVSFDNILIAWVAIFQVITLEGWTDIMYYIQDAHGFWNFIYFVVLIVIGSYFMTNLCLVVITTQFQETKQRENDLM---RQSRRK 240          

HSP 13 Score: 50.447 bits (119), Expect = 9.342e-6
Identity = 43/135 (31.85%), Postives = 62/135 (45.93%), Query Frame = 1
Query:  361 HPRDWCLKIITWPWFEQLSMTIIAVN--CITLSMYQ-PCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEI--------LPSTGNNIGLTAIRTIRVLRPLRAI 732
             PR +   + T  +F+     +IA+N  C+ L  YQ P G           ++  +Y+  A F  E ++KI A+G      Y  D WN+LDM IVI+  + I        LP     I  T IR +RVLR  R +
Sbjct: 1235 RPRKFIHAVCTHGYFDLGIAAVIALNVLCMALEHYQQPDG-------LTAFLKCANYVFTAVFILEAILKIFALGIKR---YIKDRWNQLDMLIVILSIVGIALEEMTMELP-----INPTIIRVMRVLRIARVL 1354          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO35497 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLD1])

HSP 1 Score: 495.352 bits (1274), Expect = 2.987e-157
Identity = 280/528 (53.03%), Postives = 382/528 (72.35%), Query Frame = 1
Query: 3859 QFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMK 5442
            +FRQ+ + +   ++FDY++L FI  +C  LA+E P I     +R  + ++  +FTIIFT EM+IK+++ GL++G   YL+ GW+++DGFLV +S +D+ +T     S  + G LRVFR LRTLRPLRVI RAPGLKLVVQTLL SL+PIGN VLI   FF++FGILGVQLFKGKFYYC+G     V +K +C   ++ +W N++YNFD+L QAL++LFV+S+KDGWV+IM+ GIDAV V++QP  N+ EW L+YFI FLLL  F VLNM VGVVVENF +C+E           A+  R+ +K R  ++  LY+E+Y   R  I+ + T + +D+ IA +I +NVI M+ E + MPQV    ++  NYFFTS FV+E ++K++ALG  RY  D+WN +D+ IV+LS+ G+V++ +       NPT++R +RVLRI RVLKL+K+A G+R+LLDT+ +ALPQV NLGLLFFLLF+I++ LG++LFG LECS   PC G  RHAHF +FG A LTLFRIATGDNWNGI+K
Sbjct:    7 RFRQRMRAVCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLKRQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYYCEGD--SHVISKQECANSTRGQWVNRRYNFDDLLQALISLFVVSTKDGWVEIMHHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVVVENFQRCRERM----DEEEQARPHRKGKKARNQMQDSLYYESYGPLRLKIHFICTHRNWDITIAAIICINVICMSLEHYKMPQVF---VETTNYFFTSVFVIEVVVKVIALGFVRYPKDRWNLIDLAIVLLSVTGIVLELLVKVDHLFNPTVIRTLRVLRITRVLKLVKLAKGVRSLLDTLFEALPQVPNLGLLFFLLFFIYSCLGIQLFGSLECSHDYPCQGFNRHAHFRDFGTAMLTLFRIATGDNWNGILK 525          

HSP 2 Score: 93.5893 bits (231), Expect = 2.897e-19
Identity = 76/298 (25.50%), Postives = 155/298 (52.01%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIE---YHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEG-ILG---YIKNGFNLFDGIIVVLGVIELCQQGGT-------SGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENN-----KRTCD----------RKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT----------SSWAALYFIALMTFGNYVLFNLLVAILVEGFS--SERLDEQKLLHNYRQSLKKR 2925
            +Q+++ + +   F   IL+ I+ +   + IE     +Q ++  Q ++ S +VF+I+F IEM++KL+  G +LG   Y+++G+++ DG +V++  I++     +         L + R  R LR L+++R  P L+  +  ++ ++  +     +  +F  +F ILG+ LF  KF   E +     K+ C           R NFD LL +++++F + T++ W  ++++G+             + W  +YFI  +  G +++ N++V ++VE F    ER+DE++    +R+  K R
Sbjct:    9 RQRMRAVCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLK-RQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYYCEGDSHVISKQECANSTRGQWVNRRYNFDDLLQALISLFVVSTKDGWVEIMHHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVVVENFQRCRERMDEEEQARPHRKGKKAR 305          

HSP 3 Score: 65.0846 bits (157), Expect = 1.900e-10
Identity = 41/125 (32.80%), Postives = 66/125 (52.80%), Query Frame = 1
Query:  403 FEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG------LTAIRTIRVLRPLRAINRIPSMRI 756
            F+ + +  I  +C  L++  P  D   +  K QI+     +    FT EM+IK++AMG  LG   Y  D W+ LD F+V+V  I+I+ +  +++       L   R +R LRPLR I R P +++
Sbjct:   21 FDYVILVFIIFSCAVLAIEAP--DIAEQGLKRQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKL 143          

HSP 4 Score: 57.3806 bits (137), Expect = 5.969e-8
Identity = 65/240 (27.08%), Postives = 121/240 (50.42%), Query Frame = 1
Query: 2083 YSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGV--------IELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS 2775
            + K ++  N+ Q+    ++Y  + + KI  +     +   I   I IN + M +E+++ P    +T   +N  F+ +F+IE+++K+I  G + Y K+ +NL D  IV+L V        +++      + +  LR  R+ R+LKLV+    +R  L  +   +  V     L  L  FI+S LG+ LFG   C+ +   +  +R  +F     +++T+F+I T ++WN +L  G T T +
Sbjct:  298 HRKGKKARNQMQDSLYYESYGPL-RLKIHFICTHRNWDITIAAIICINVICMSLEHYKMPQVFVET---TNYFFTSVFVIEVVVKVIALGFVRYPKDRWNLIDLAIVLLSVTGIVLELLVKVDHLFNPTVIRTLRVLRITRVLKLVKLAKGVRSLLDTLFEALPQVPNLGLLFFLLFFIYSCLGIQLFGSLECSHDYPCQGFNRHAHFRDFGTAMLTLFRIATGDNWNGILKVGPTNTVT 533          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO35498 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLD2])

HSP 1 Score: 283.493 bits (724), Expect = 7.802e-81
Identity = 181/340 (53.24%), Postives = 230/340 (67.65%), Query Frame = 1
Query:  400 WFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKC-SDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERM 1416
            WFE++SM II VNCITL+MY P  D+ CE  +C +++ F++ IF FF  EMV+K++AMG  G  GY  DTWN+LD  I+++G IE   S   +  LT IR  RVLRPLRAIN++PS+RILV LLLDTLPMLGNVLLL F VFF+FGIIGVQ+WQG LRNRC+ + N++      N S FY+ +       +  DF+CS   N G+  C SDIP S +    +  Q C  P    T N     +C +WN  Y DC+    NP+ G I+FDNI  AW+ IFQVI++E W D+MY VQD HSFW+WIYF+ LI+IG+FF++NLCLVVI  QF ETK RE E M
Sbjct:    4 WFERISMVIILVNCITLAMYDPL-DKRCEKVRCNVLEKFEFFIFGFFLLEMVVKMVAMGVFGSHGYLQDTWNKLDFVIIVMGVIE--KSLQGSDYLTIIRAFRVLRPLRAINKVPSIRILVTLLLDTLPMLGNVLLLSFLVFFVFGIIGVQLWQGKLRNRCFASTNDTALLLSFNQSVFYKPS------FIQPDFVCSGPTNTGMTSCLSDIPHSYL----HGRQ-CTLPPENLTSN---SSNCADWNSLYRDCREDGPNPFWGTISFDNIAIAWMVIFQVITLEGWSDVMYLVQDTHSFWNWIYFIILIVIGAFFLVNLCLVVITMQFQETKAREIELM 326          

HSP 2 Score: 212.231 bits (539), Expect = 7.005e-57
Identity = 133/249 (53.41%), Postives = 166/249 (66.67%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTI-ENNKRTCDRKNFDSLLWSIVTVFQ----------------------ILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSS 2868
            F R I+IAIL+N + MG+E+H QP  LT TLE +NIVF  +F++EMI+ +I  GI+GY+    N+FDG +VVL V EL  + G + LS+ R+ RLLRI KLVR    +R QL V+IRT+ +V TFF LL LF+F F+ILGM+LFG +F      N     R NFDS LW++VTVFQ                      ILTQE+WN V++ GM  TS WAALYFIALMT G YVLFNLLVAILVEGF+S
Sbjct:  485 FSRLIMIAILLNMICMGLEHHNQPQALTVTLEKTNIVFVTIFVLEMIINVISFGIMGYLSQLQNIFDGFVVVLSVTELLLENGYARLSVFRSIRLLRIFKLVR---PVRYQLLVVIRTMTSVVTFFGLLFLFMFAFAILGMNLFGGEFYFPNAENISVPARTNFDSFLWAMVTVFQVRRGSCHGDDVPYEKKIIAMVTILTQENWNQVMFKGMRATSYWAALYFIALMTVGYYVLFNLLVAILVEGFTS 730          

HSP 3 Score: 90.1225 bits (222), Expect = 4.944e-18
Identity = 75/289 (25.95%), Postives = 146/289 (50.52%), Query Frame = 1
Query: 3847 SVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSN-RIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQA-VKTKNDCLKYSQN---EWKNQKYNFDNLG--QALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
             V ++FR  C+ + + + F  L++  I LN I + +E  + P      + L  TN +F  IF  EMII V+S G++     YL    NI DGF+V +S+ +L +    G++   +F  +R+ R+ + +RP+R       L +V++T+ S    +     +   F   F ILG+ LF G+FY+ +  ++    +T  D   ++     + +    + D++   + ++A+  + +++ W Q+M+ G+ A           + WA +YFI+ + +  + + N+ V ++VE F
Sbjct:  467 GVVHRFRAFCRRMAESRHFSRLIMIAILLNMICMGLEHHNQP--QALTVTLEKTNIVFVTIFVLEMIINVISFGIM----GYLSQLQNIFDGFVVVLSVTELLLEN--GYARLSVFRSIRLLRIFKLVRPVRY-----QLLVVIRTMTS----VVTFFGLLFLFMFAFAILGMNLFGGEFYFPNAENISVPARTNFDSFLWAMVTVFQVRRGSCHGDDVPYEKKIIAMVTILTQENWNQVMFKGMRAT----------SYWAALYFIALMTVGYYVLFNLLVAILVEGF 728          

HSP 4 Score: 80.8777 bits (198), Expect = 4.263e-15
Identity = 54/165 (32.73%), Postives = 87/165 (52.73%), Query Frame = 1
Query: 3901 FDYLVLFFITLNCITLAMERPSIPPHSKERL-FLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIF---GILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF 4383
            F+ + +  I +NCITLAM  P      K R   L    +     F  EM++K+++ G+  G   YL+  WN +D           F+ I++G   +       L + R  R LRPLR I++ P ++++V  LL +L  +GN++L+    F +FGI+GVQL++GK 
Sbjct:    5 FERISMVIILVNCITLAMYDPLDKRCEKVRCNVLEKFEFFIFGFFLLEMVVKMVAMGVF-GSHGYLQDTWNKLD-----------FVIIVMGVIEKSLQGSDYLTIIRAFRVLRPLRAINKVPSIRILVTLLLDTLPMLGNVLLLSFLVFFVFGIIGVQLWQGKL 157          

HSP 5 Score: 50.447 bits (119), Expect = 7.762e-6
Identity = 26/77 (33.77%), Postives = 52/77 (67.53%), Query Frame = 1
Query: 2326 MILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            M++K++  G+ G   Y+++ +N  D +I+V+GVIE   Q G+  L+I+R FR+LR L+ +  +P++R  + +++ T+
Sbjct:   53 MVVKMVAMGVFGSHGYLQDTWNKLDFVIIVMGVIEKSLQ-GSDYLTIIRAFRVLRPLRAINKVPSIRILVTLLLDTL 128          

HSP 6 Score: 50.0618 bits (118), Expect = 9.927e-6
Identity = 60/236 (25.42%), Postives = 105/236 (44.49%), Query Frame = 1
Query: 4813 KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATG----------------------DNWNGIMKDTLR 5454
            ++R     +  S++F   I   I LN+I M  E H  PQ L   L+  N  F + FVLE ++ +++ G+  Y+S   N  D  +V+LS+  ++++   +            + V R  R+L++ K+   +R  L  VI+ +  V     L FL  + FA LG+ LFG          + +    +F++F  A +T+F++  G                      +NWN +M   +R
Sbjct:  471 RFRAFCRRMAESRHFSRLIMIAILLNMICMGLEHHNQPQALTVTLEKTNIVFVTIFVLEMIINVISFGIMGYLSQLQNIFDGFVVVLSVTELLLENGYAR-----------LSVFRSIRLLRIFKLVRPVRYQLLVVIRTMTSVVTFFGLLFLFMFAFAILGMNLFGGEFYFPNAENISVPARTNFDSFLWAMVTVFQVRRGSCHGDDVPYEKKIIAMVTILTQENWNQVMFKGMR 695          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO46991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNU9])

HSP 1 Score: 231.491 bits (589), Expect = 8.327e-61
Identity = 187/563 (33.21%), Postives = 309/563 (54.88%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYC-DGPHLQAVKTKNDCLKY----------SQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLR--APLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVI---QALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIM---KDTLREKC 5463
            SL++FS  N FR  C  I     F   +L  I ++   LA E P +  +SK    L   +Y FT +FT E+ IK+++ G+++   ++ +S +N++D  +V +S+    ++I L  S+    ++R+ R+LR LRPLR I+RA GLK VVQ +  +++ IGNI+L+   F  +F ++GVQLFKG F+ C D   +   + +   +++             EW+ Q +NF+++ QA++ LF + + +GW  I+ + +D+  V+  P  N+  W  IY++ +++++AFF++N+FVG V+  F    E + K+ +  +  ++      K +  R   P     +  WR     VVTS+ F+  I   I  N I +  +++  P++    L  FN  FTS F+LE +LKL A     Y  D+WN  D ++V+ S+  + ++EV S  M          R+ R  R++KLL   SGI+ LL T I   QALP VG   LL  + F+I+A +G+++FGR+    I+P   + R+ +F+ F  + + LFR ATG+NW  IM    DT   KC
Sbjct:  812 SLFIFSNTNCFRVVCHRIATNSYFVNFILLLIIVSSCMLAAEDP-LNSNSKRNQVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSI----VSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLFKGTFFSCTDAEKITKRECQGQYIEFKGPGLTNPVVKDREWQPQTFNFNDVPQAMLTLFTVMTFEGWPGILESSMDSTDVDEGPFLNNRPWVAIYYVIYIIIIAFFMINIFVGFVIVTFQNEGEEEFKDCELDKNQRKCVEFALKARPTRRYIPTNRLQFHVWR-----VVTSQPFEYLIFAFITGNTILLMMQYYNEPKLYTRVLDGFNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQM----FAFGFFRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVG---LLIIMTFFIYAVVGMQMFGRIA---IDPETQINRNNNFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKC 1354          

HSP 2 Score: 150.214 bits (378), Expect = 4.328e-36
Identity = 101/344 (29.36%), Postives = 168/344 (48.84%), Query Frame = 1
Query: 3781 SKCCPQPK----GIFAKRYYHSLYVFSVDNQFRQKCQHI-------VDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLF---LTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYC-----------DGPHLQAVKTKN----------DCLKYSQNEWKNQKY---NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHK 4698
             K  PQPK       A R   +L+  ++ N  R     +       V +  FD ++L  I  NC  LA  +P   P     L    L V  ++F  IFT E ++K+++ G ++   AYL++GWNI+D  +V + L  + + +    S  +    +  R  R LRPLR++S  P L++V+ +++ +L P+ +I L+     II+ I+GV+LF GK +             D PH  + +++            CLK    +W+   Y   NFDN+G A + +F   + +GW  +MY+  DA+G         N W  +YF++ ++  +FFVLN+ +GV+   F K
Sbjct:   25 GKPPPQPKRQKKPTNAVRPKRALFCLTLGNPIRSTAISLFTVFFLDVFRTPFDVMILITIFANCAALAAFQPL--PEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDV----KALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQPTFDEPHPCSTESEGYSCSNAGPGQVCLK----KWEGPNYGITNFDNIGLACLTVFQCITLEGWTDVMYSINDAIG---------NSWPWLYFVTLIIWGSFFVLNLVLGVLSGEFAK 349          

HSP 3 Score: 137.887 bits (346), Expect = 2.677e-32
Identity = 121/412 (29.37%), Postives = 188/412 (45.63%), Query Frame = 1
Query:  178 PQDNRSRRETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLG-YFGDTWNRLDMFIVIVGSIEILPS--TGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA-HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRR 1401
            PQ  R ++ T        N     +A  C   T G PI      ++  F+  VF      + P            F+ + +  I  NC  L+ +QP  +Q+      ++  A +++    FT E V+KIIA GF+   G Y  + WN LD  IV+VG   I+    T ++  + A+R  RVLRPLR ++ +PS+++++  ++  L  L ++ LL  FV  I+ IIGV+++ G L + CY N+                          D+   CST+       CS+  P          Q+C +                 W           + P  G  NFDNIG A + +FQ I++E W D+MY + DA  + W W+YFV LII GSFF++NL L V++ +F++ K R
Sbjct:   29 PQPKRQKKPT--------NAVRPKRALFCL--TLGNPIRSTAISLFTVFFLDVF------RTP------------FDVMILITIFANCAALAAFQPLPEQDSSLINEELEVA-EFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQ--------------------PTFDEPHPCSTESEG--YSCSNAGPG---------QVCLK----------------KW-----------EGPNYGITNFDNIGLACLTVFQCITLEGWTDVMYSINDAIGNSWPWLYFVTLIIWGSFFVLNLVLGVLSGEFAKEKAR 353          

HSP 4 Score: 137.502 bits (345), Expect = 3.814e-32
Identity = 91/252 (36.11%), Postives = 145/252 (57.54%), Query Frame = 1
Query: 2152 CQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTS----GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAA----------LYFIALMTFGNYVLFNLLVAILVEGFS 2865
            C++ +KT      F   +++ + +N+L++ +E++ QP  LTQ L+ +N +F  LF +EM++K+   G  GY  + FN FD ++V+  ++EL           G+S+LR  RLLRI K+ R+  +L   +  ++ ++ ++     LL LF+ IFS+LGM +FG KF   + +     R NFDS   ++VTVFQILT EDWN V+Y G+    SW            LYFI L+  GNY+L N+ +AI V+  +
Sbjct:  451 CRKAVKTQW----FYWTVIVFVFLNSLTLALEHYNQPEFLTQFLDKANKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISSLLELGLTEAMDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGGKFNLGDED---VPRSNFDSFWRALVTVFQILTGEDWNAVMYTGI---QSWGGITESLSAIPILYFIFLVVVGNYILLNVFLAIAVDNLA 692          

HSP 5 Score: 112.079 bits (279), Expect = 1.794e-24
Identity = 87/296 (29.39%), Postives = 147/296 (49.66%), Query Frame = 1
Query: 3817 KRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQ 4704
            KR++H L         R+ C+  V  + F + V+ F+ LN +TLA+E  + P    +  FL   N +F  +FT EM++K+      +G   Y  S +N  D  +V  SLL+L +T  +        +LR  RLLR  +  R  S    L  +V +LL+S+R I  ++L+   F +IF +LG+Q+F GKF   D           D            + NFD+  +AL+ +F + + + W  +MYTGI + G      E+ +   ++YFI  +++  + +LN+F+ + V+N    +
Sbjct:  441 KRFHHRL---------RRSCRKAVKTQWFYWTVIVFVFLNSLTLALEHYNQPEFLTQ--FLDKANKLFLALFTLEMVVKMYC----LGFHGYFASLFNRFDCLVVISSLLELGLTEAMDQRPIGISMLRCVRLLRIFKVTRYWS---SLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGGKFNLGD----------EDV----------PRSNFDSFWRALVTVFQILTGEDWNAVMYTGIQSWG---GITESLSAIPILYFIFLVVVGNYILLNVFLAIAVDNLADAE 695          

HSP 6 Score: 91.6633 bits (226), Expect = 2.772e-18
Identity = 74/287 (25.78%), Postives = 135/287 (47.04%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGV----NIQPQENHN----EWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            N+ +     +V  + F+YL+  FIT N I L M+      +++ +L+  V +  F I FT   +++ + K      + Y    WN+ D  +V  S++D+ +  +       FG    FRL R LR ++++++  G+K ++ T + S + +  + L+    F I+ ++G+Q+F G+      P  Q                 N+  NF    Q+LM LF  ++ + W  IM    D        N  PQ+       ++A  YF SF  + +F ++N+FV V+++NF
Sbjct: 1141 NRLQFHVWRVVTSQPFEYLIFAFITGNTILLMMQY-----YNEPKLYTRVLDG-FNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQMFAFGF---FRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMF-GRI--AIDPETQI----------------NRNNNFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKCDPNAYPQDTDGLCGTDFAYAYFCSFYAICSFLIINLFVAVIMDNF 1399          

HSP 7 Score: 90.8929 bits (224), Expect = 5.346e-18
Identity = 77/282 (27.30%), Postives = 136/282 (48.23%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQ----LTQTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTS---GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF-------------------CTIENNKRTCDRK------------------NFDSLLWSIVTVFQILTQEDWNTVLYN-GMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQK 2889
            F   ILI I  N  ++   +   P Q    + + LE +  VF  +F +E +LK+I  G +     Y++NG+N+ D +IVV+G+  +  +  T     +  LR FR+LR L+LV  +P+L+  L  +I+ +  +     L++  + I++I+G+ LF  K                    C+ E+   +C                     NFD++  + +TVFQ +T E W  V+Y+      +SW  LYF+ L+ +G++ + NL++ +L   F+ E+   QK
Sbjct:   76 FDVMILITIFANCAALA-AFQPLPEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQPTFDEPHPCSTESEGYSCSNAGPGQVCLKKWEGPNYGITNFDNIGLACLTVFQCITLEGWTDVMYSINDAIGNSWPWLYFVTLIIWGSFFVLNLVLGVLSGEFAKEKARAQK 356          

HSP 8 Score: 70.8626 bits (172), Expect = 6.309e-12
Identity = 75/251 (29.88%), Postives = 111/251 (44.22%), Query Frame = 1
Query:   55 KKSENK-VERKKYSLDSFPSSVF---YEANEFLEIENEIECLDILPQDNRSRRETLIGNKSDFNLTDKSKA----DH-------CQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYF-GDTWNRLDMFIVIVGSIEI-LPSTGNNIGLTAI-RTIRVLRPLRAINRIPSMR 753
            + S NK +E K+ S  S  S      ++ NE L +  +I         +R R+  +   +S  +L + SKA    DH         +  D  P+ P  SL           IF      R  C +I T  +F    + +I V+   L+   P    N  + + Q++  FDY   A FT E+ IKIIA G +   G F    +N LD  +V V  + I L  + + I +  I R +RVLRPLRAINR   ++
Sbjct:  722 ESSSNKDLENKRASTSSGKSHRLIKIWKTNELLHLAGKIRV-------SRLRKHPITDAESP-SLAEDSKATLNRDHESVRSATSTEVMDHEPMPPESSLF----------IFSNTNCFRVVCHRIATNSYFVNFILLLIIVSSCMLAAEDPL---NSNSKRNQVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLK 951          

HSP 9 Score: 66.2402 bits (160), Expect = 1.570e-10
Identity = 71/285 (24.91%), Postives = 134/285 (47.02%), Query Frame = 1
Query: 2107 NKNQNK----------TKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTS----GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLY-------------------NGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGF 2862
            +KNQ K          T+R    +  Q  +  +V    F+  I   I  NT+ + ++Y+ +P   T+ L+  NI F+ +FL+E ILKL       Y  + +NLFD ++VV  V+++     +S         R FR LR++KL+     ++  L+  I++   +     L+I+  FI++++GM +FG     I+   +     NF +   S++ +F+  T E+W  ++                    +G+  T  +A  YF +     ++++ NL VA++++ F
Sbjct: 1118 DKNQRKCVEFALKARPTRRYIPTNRLQFHVWRVVTSQPFEYLIFAFITGNTILLMMQYYNEPKLYTRVLDGFNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQMFAFGFFRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMFGR--IAIDPETQINRNNNFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKCDPNAYPQDTDGLCGT-DFAYAYFCSFYAICSFLIINLFVAVIMDNF 1399          

HSP 10 Score: 63.1586 bits (152), Expect = 1.575e-9
Identity = 66/246 (26.83%), Postives = 126/246 (51.22%), Query Frame = 1
Query: 4807 YSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG---RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIF 5535
            + + RR+    V +++F   +   + LN +T+A E +  P+ L   L   N  F + F LE ++K+  LG   Y +  +N+ D ++VI S+  + + E     M   P  + ++R +R+ R+ K+ +  S +  L+ +++ ++  +  L LL  L   IF+ LG+++FG    L   D+         ++F++F  A +T+F+I TG++WN +M   ++     +E       +SAI  PI + IF
Sbjct:  444 HHRLRRSCRKAVKTQWFYWTVIVFVFLNSLTLALEHYNQPEFLTQFLDKANKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISSLLELGLTEA----MDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGGKFNLGDEDVP-------RSNFDSFWRALVTVFQILTGEDWNAVMYTGIQSWGGITES------LSAI--PILYFIF 670          

HSP 11 Score: 62.003 bits (149), Expect = 3.562e-9
Identity = 53/203 (26.11%), Postives = 104/203 (51.23%), Query Frame = 1
Query: 2275 QTLEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALR------QQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN--NKRTC------------------DRK------NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT 2769
            Q L + +  F+ +F IE+ +K+I  G++     + ++ FNL D ++V + ++ +  +  +S +S++R  R+LR+L+ +R +   +      Q +FV ++TI N+     + +LF F+F+++G+ LF   F  CT      KR C                  DR+      NF+ +  +++T+F ++T E W  +L + M  T
Sbjct:  864 QVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNI---MLVTVLFNFLFAVIGVQLFKGTFFSCTDAEKITKRECQGQYIEFKGPGLTNPVVKDREWQPQTFNFNDVPQAMLTLFTVMTFEGWPGILESSMDST 1063          

HSP 12 Score: 52.7582 bits (125), Expect = 2.059e-6
Identity = 34/120 (28.33%), Postives = 58/120 (48.33%), Query Frame = 1
Query:  367 RDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNN---IGLTAIRTIRVLR 717
            R  C K +   WF    +  + +N +TL++       N      Q +   + +  A FT EMV+K+  +GF G   YF   +NR D  +VI   +E+  +   +   IG++ +R +R+LR
Sbjct:  448 RRSCRKAVKTQWFYWTVIVFVFLNSLTLALEH----YNQPEFLTQFLDKANKLFLALFTLEMVVKMYCLGFHG---YFASLFNRFDCLVVISSLLELGLTEAMDQRPIGISMLRCVRLLR 560          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Nematostella
Match: EDO35661 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKR1])

HSP 1 Score: 230.72 bits (587), Expect = 8.918e-61
Identity = 200/640 (31.25%), Postives = 341/640 (53.28%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYC---DGPHLQA--VKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXX----------XXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNC-------CVSAIIAPIY---FVIFXXXXXXXXXXXXXXXXXKHLEESHKMMC--DDYELEREVQRDLDEEAKELAREKQLKD 5703
            R K + +V+ K F+ ++LF I  + I+LA E   +      +  L + N +F +IFT EM++K +     +G + Y  + WNI+D  +V +S++    TI     N     +R  R LR  RPLR ISR  G+K+V+ +LL ++  IGN++L+C  F++IF I+GVQ+F GKF  C    G    A  V  K  CL  +   W+N   NFD + Q  +AL  +++ +GW++IM   +DA  ++ QP E +N  A ++F+ F++L  FF LN+F+GV+++NF++ ++  E                         +K +K +R P      +K++  +  +V S+ F++ I   I +N++ M  + +    V     ++ N  FTS F+LEA+L+++AL    Y  + WN  D +IVI SI G++++ ++++ + INP+++RV+RV R+ R+L+  + A GIR LL +++ + P + N+G L FL+ +I+A +G+ +FG ++ ++      L    +FE FG +F+ LFR+ T   WN    D L    IS  DC  N        C +  +APIY   FV+ + +    +   V+      + E  K+    +D EL  ++    D  A +      L D
Sbjct:  817 RYKVRRLVEHKVFEGIILFLIAASSISLAFEDVYLDSKPTLKQVLQILNILFAVIFTVEMLLKWIG----LGFKTYFTNPWNILDFVIVIVSIMVSLATIF---GNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKFGKCVDEGGEKCPASVVPNKTVCLS-NGYRWENSNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKIDEQPIEENNVSAYLFFVVFIILGTFFTLNLFIGVIIDNFNQLKQQMEAVGSMDVFLTSTQRNWMNALKSAATKKPKKRIRRP-----QNKFQGELYDMVQSRKFEVFIMLFITINMVVMMVQHYDHLAVWSK--QILNLIFTSVFILEAILRIIALRKG-YFLNPWNVFDFVIVISSIIGIIVENLQTSLV-INPSLLRVVRVFRVGRLLRFFEAARGIRRLLFSLVISTPALFNIGALLFLIIFIYAIIGMSIFGHVKKTE-----ALNDVVNFETFGSSFVLLFRLMTSAGWN----DILDPLMISEPDCDPNYRGLPSGNCGNKYMAPIYLGSFVVIIFLILINMYIAVILENYNQVMEQEKIGITNEDIELFYQMWELFDPNATQYIPYADLSD 1430          

HSP 2 Score: 123.25 bits (308), Expect = 7.777e-28
Identity = 86/282 (30.50%), Postives = 150/282 (53.19%), Query Frame = 1
Query: 2020 VASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRG------ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIE-----LCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFN 2832
            + S+ S++N+ +  I       S H+   N +  K ++K    + ++  +     +KF  G      I++ IL+NTL M IE+ +    L   +  SN VF+ +FL+EMILKLI  G LGYI+  +N+FDGI+V++ +++          G +G+S+LRTFRLLR+LKL +    +   L  + +++  +     +L + ++IF+++GM L G  + T +    +  R NF     S + +F++L  E W   L++ M  T   A L F+     GN+++ N
Sbjct:  449 IGSQKSNLNNASQII-------SSHKSSLNLSVKKEEKKRSSIMARRWKRFRRRLLKFTNGKVMEIFIIVCILLNTLVMSIEHPRLEDPLLTVVNISNEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSY-TPDKFGGSIPRWNFKDFPHSFMMIFRVLCGE-WIEPLWDCMLATGPVAMLLFVPAFILGNFIILN 721          

HSP 3 Score: 108.227 bits (269), Expect = 2.633e-23
Identity = 114/379 (30.08%), Postives = 174/379 (45.91%), Query Frame = 1
Query:  340 HIFHQNKHPRDWCLKIITWPWFEQLS------MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFS---ETKRRETERMWIERRRFNSS 1446
            ++F      R + LK+IT  +  QL       +  I VNC+ L+M  P                 +Y+  A +T EM  KIIA GF L    Y  D WN LD  +VI+G++ I P   N   L+ I T RV R LR I+ +  ++ +V  LL ++ ML +V++L  F   IF +IG+Q++ G LRN+C L + E       NLS  Y   RT   N+                       SI  L +N   +C       +   PT ++C+              NP  G  +FD+ G+A +  FQ+I+++ W ++   V      W  +YF  +I  G FF++NL L V+A+ +    +  + ETE    E++R  SS
Sbjct:   25 YLFGPENPIRQFSLKLITNQYPLQLHNRHMFVLLTILVNCVFLAMTNPPEQP-------------EYVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVTISPDVAN---LSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGELRNKCALPVPE-------NLSVPY---RTYASNS-----------------------SIWYLEDNEPILCGNTTG--SRTCPTNYTCL---------PHAGGNPNFGYTSFDHFGWALLTGFQLITLDFWENVYNNVIYTMGPWYVVYFAVVIFFGPFFLVNLVLAVVAASYENEVKASKMETEEERKEQKRVASS 343          

HSP 4 Score: 95.9005 bits (237), Expect = 1.508e-19
Identity = 89/336 (26.49%), Postives = 141/336 (41.96%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKC------QHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSN------RIFGI--LRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKF-------------------------YYCDG--PHL-------QAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            SLY+F  +N  RQ        Q+ +   +    VL  I +NC+ LAM  P   P            Y+F  I+T EM  K+++KG  +   AYL+  WN +D           FI ++LG+         + GI   RVFR LRT      IS   GLK +V TLL S++ + +++++   F  IF ++G+QLF G+                          +Y +   P L       +   T   CL ++         +FD+ G AL+  F L + D W  +    I  +G           W ++YF   +    FF++N+ + VV  ++ 
Sbjct:   23 SLYLFGPENPIRQFSLKLITNQYPLQLHNRHMFVLLTILVNCVFLAMTNPPEQPE-----------YVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLD-----------FIVVILGNVTISPDVANLSGIPTFRVFRALRT------ISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGELRNKCALPVPENLSVPYRTYASNSSIWYLEDNEPILCGNTTGSRTCPTNYTCLPHAGGNPNFGYTSFDHFGWALLTGFQLITLDFWENVYNNVIYTMG----------PWYVVYFAVVIFFGPFFLVNLVLAVVAASYE 320          

HSP 5 Score: 81.2629 bits (199), Expect = 4.122e-15
Identity = 68/276 (24.64%), Postives = 130/276 (47.10%), Query Frame = 1
Query: 2149 ICQQKIKTLVNDVKFQRGILIAILINTLSMGIE--YHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGV-IELCQQGGTSGLSILRTFRLLRILKLVRFMP---ALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CTIEN----------NKRTC---------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT----------SSWAALYFIALMTFGNYVLFNLLVAILVEGFS 2865
            + + K++ LV    F+  IL  I  +++S+  E  Y      L Q L+  NI+F+++F +EM+LK IG G   Y  N +N+ D +IV++ + + L    G   ++ +R+ R LR  + +R +     ++  +  ++  I  +     + ++F  IFSI+G+ +FG KF  C  E           NK  C            NFD++    + + Q+ T E W  ++ + +  T          +  A L+F+  +  G +   NL + ++++ F+
Sbjct:  815 VMRYKVRRLVEHKVFEGIILFLIAASSISLAFEDVYLDSKPTLKQVLQILNILFAVIFTVEMLLKWIGLGFKTYFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKFGKCVDEGGEKCPASVVPNKTVCLSNGYRWENSNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKIDEQPIEENNVSAYLFFVVFIILGTFFTLNLFIGVIIDNFN 1090          

HSP 6 Score: 77.411 bits (189), Expect = 6.706e-14
Identity = 69/262 (26.34%), Postives = 121/262 (46.18%), Query Frame = 1
Query: 3889 DKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNY---IFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRI-FGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLN 4662
            + K  +  ++  I LN + +++E P +     E   LTV N    +FT IF  EMI+K+++ G L     Y++  WNI DG +V IS++D  +   +  +      +LR FRLLR L+  +  S    L   + T+  SL  +GN+ +I      IF ++G+QL  G  Y  D              K+  +     ++NF +   + M +F +   + W++ ++  + A G            A++ F+   +L  F +LN
Sbjct:  501 NGKVMEIFIIVCILLNTLVMSIEHPRL-----EDPLLTVVNISNEVFTFIFLLEMILKLIALGFL----GYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSL---LATIGKSLGALGNLTVILAIIVYIFAVMGMQLL-GNSYTPD--------------KFGGSI---PRWNFKDFPHSFMMIFRVLCGE-WIEPLWDCMLATG----------PVAMLLFVPAFILGNFIILN 721          

HSP 7 Score: 70.8626 bits (172), Expect = 6.132e-12
Identity = 69/305 (22.62%), Postives = 123/305 (40.33%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGI----LGYIKNGFNLFDGIIVVLGVIELCQQ-GGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLF------GCKFCTIEN-----------------------------NKRTC----------------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLR 2949
            +L+ IL+N + +          +T   E    VF+ ++ IEM  K+I +G       Y+++ +N  D I+V+LG + +       SG+   R FR LR +  V+ + A+   L V ++ + +V       I    +    GM LF       C     EN                               RTC                   +FD   W+++T FQ++T + W  V  N +     W  +YF  ++ FG + L NL++A++   + +E +   K+     +  +KR A++  +R
Sbjct:   56 VLLTILVNCVFLA---------MTNPPEQPEYVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVTISPDVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALI---GMQLFIGELRNKCALPVPENLSVPYRTYASNSSIWYLEDNEPILCGNTTGSRTCPTNYTCLPHAGGNPNFGYTSFDHFGWALLTGFQLITLDFWENVYNNVIYTMGPWYVVYFAVVIFFGPFFLVNLVLAVVAASYENE-VKASKMETEEERKEQKRVASSYSVR 347          

HSP 8 Score: 63.5438 bits (153), Expect = 1.137e-9
Identity = 66/193 (34.20%), Postives = 107/193 (55.44%), Query Frame = 1
Query: 4846 SKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMP-INPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGD 5421
             K  ++ I   I LN + M+ E   +   L T + + N  FT  F+LE +LKL+ALG   YI   WN  D ++VI+SI    +D + + F+P    T + V+R  R+ RVLKL K  S + +LL T+ ++L  +GNL ++  ++ YIFA +G++L G     D     G     +F++F  +F+ +FR+  G+
Sbjct:  502 GKVMEIFIIVCILLNTLVMSIEHPRLEDPLLTVVNISNEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISI----VDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSYTPD--KFGGSIPRWNFKDFPHSFMMIFRVLCGE 688          

HSP 9 Score: 61.2326 bits (147), Expect = 5.308e-9
Identity = 40/121 (33.06%), Postives = 65/121 (53.72%), Query Frame = 1
Query:  403 FEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLT---AIRTIRVLRPLRAINRIPSMRI 756
            FE + + +IA + I+L+      D   + +  Q++Q  + +    FT EM++K I +GF     YF + WN LD  IVIV  +  L +   N  +    ++RT+R  RPLRAI+R   M++
Sbjct:  829 FEGIILFLIAASSISLAFEDVYLDS--KPTLKQVLQILNILFAVIFTVEMLLKWIGLGFKT---YFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKV 944          

HSP 10 Score: 57.3806 bits (137), Expect = 7.283e-8
Identity = 68/304 (22.37%), Postives = 142/304 (46.71%), Query Frame = 1
Query: 3856 NQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMER-PSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLL-----DLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWAL------------IYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N+F+ +   +V  + F+  ++ FIT+N + + ++    +   SK+     + N IFT +F  E I+++++       + Y  + WN+ D  +V  S++     +L  ++++  S       RV R+ R  R LR    A G++ ++ +L+ S   + NI  +      I+ I+G+ +F          H++  +  ND +            NF+  G + + LF L +  GW  I+    D + ++ +P  + N   L            IY  SF++++   ++NM++ V++EN+++  E +
Sbjct: 1134 NKFQGELYDMVQSRKFEVFIMLFITINMVVMMVQHYDHLAVWSKQ-----ILNLIFTSVFILEAILRIIAL-----RKGYFLNPWNVFDFVIVISSIIGIIVENLQTSLVINPSLL-----RVVRVFRVGRLLRFFEAARGIRRLLFSLVISTPALFNIGALLFLIIFIYAIIGMSIFG---------HVKKTEALNDVV------------NFETFGSSFVLLFRLMTSAGWNDIL----DPLMIS-EPDCDPNYRGLPSGNCGNKYMAPIYLGSFVVIIFLILINMYIAVILENYNQVMEQE 1396          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1ha (voltage-dependent T-type calcium channel subunit alpha-1H [Source:NCBI gene;Acc:101161560])

HSP 1 Score: 645.195 bits (1663), Expect = 0.000e+0
Identity = 393/617 (63.70%), Postives = 482/617 (78.12%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQ---RNC-CVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEE 5667
            SLY+F  ++ FR  CQ ++  K FD++VLFFI LNCIT+AMERP I PHS ERLFL+ +NYIFT+IF  EM IKV++ G   G Q YL+  WNI+DG LVF+SLLD+ ++I     N+I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICCTFFI+FGILGVQLFKGKFY C+G   + V  K+DCL+ +   W N+KYNFDNL +ALM+LFVLS KDGWV IMY G+DAVGV+ QP  NHN W LIYFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+EE   R  KR + ++KKR+      Y+ +YS  R  I+ + T+ Y DL I  ++G+N++TM+ E +   Q LK ALK  NY FT  F +EALLKLVA G+ R+  DKWNQLD+ IV LSI G+V++E+     +PINPTI+R+MRVLRIARVLKLLKMA G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L+C+DINPC GL RHA FENFGMAFLTLFR++TGDNW+GIMKDTLRE       CQ   RNC    A ++P YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E+  +++ E
Sbjct: 1144 SLYLFDPESWFRSCCQRVISHKLFDHVVLFFIFLNCITIAMERPDIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVSIAYVGENKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCTFFIVFGILGVQLFKGKFYICEGFDTRNVTNKSDCLQ-ANYRWTNRKYNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRRAMQRPYYADYSPVRLYIHRLCTNHYLDLFITLIVGINILTMSVEHYNQQQYLKEALKDCNYVFTFIFFVEALLKLVAFGIHRFFKDKWNQLDVAIVALSILGIVLEELNMKGALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLKCTDINPCEGLSRHATFENFGMAFLTLFRVSTGDNWSGIMKDTLRE-------CQPDDRNCFAYLAWVSPFYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIALEMERE 1752          

HSP 2 Score: 335.109 bits (858), Expect = 4.562e-92
Identity = 204/383 (53.26%), Postives = 267/383 (69.71%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFT--DNLP--TKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLY 1554
            M +I +NC+TL M+QPC D +C + +C+++Q  D  IFAFF  EMVIK++A G  G   Y  D WN+LD  IV+ G +E     G N  L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVL LCFFVFFIFGI+GVQ+W G+LRNRC+L  +    +   +L  +Y           D  FICST + NG+L C D+PPS       SL   +Q +      D++   + + CVNWNQYY  C  GE NP++GA+NFDNIG+A +AIFQVI++E WVDIMY+V DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE   M  +R R+ SS+ +  S  EP +CY EI++ I+ +  K+ RR+ ++Y
Sbjct:    1 MMVILLNCVTLGMFQPCEDVDCLSERCKVLQVLDDCIFAFFAVEMVIKMMAFGVFGSKCYLADRWNQLDFVIVMAGMLE-YSLDGQNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLFLCFFVFFIFGIVGVQLWAGLLRNRCFLGEDTKVLY-NQSLKPYYVDEE-----GEDNPFICSTPRENGMLHCYDVPPSTEDGVECSLTASQQSSVLIGSFDSVAGASVNGCVNWNQYYNVCHAGEFNPHKGAVNFDNIGYASIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSSDSTFSSYTEPGSCYEEILRYISHLYRKLRRRLQRIY 376          

HSP 3 Score: 318.546 bits (815), Expect = 6.308e-87
Identity = 179/315 (56.83%), Postives = 227/315 (72.06%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF-CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVL-LTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSS 3093
            ++ ++T+V    F + I+ AILINT SMGIEYH+QP  LTQ LE +N VF+ +F++EM   L+  G+ GYIKN +N+FD ++V++ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIF FSILGM LFGCKF   +EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS WAALYF+ALMTFGNYVLFNLLVAILVEGF +E         N  +S  +++++N        Q  ++ L +T +   D  G     ++ PP+I+ TAATPMPTP SS
Sbjct:  645 RETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQVLEITNFVFTSIFVLEMGFMLLAFGLWGYIKNPYNIFDSVVVIISVWEISAQA-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFGLRLENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEG------DANRSESDDEKKSDNSDEDEKLEQFTDIELEMTANGHIDACG-----TTAPPIIMRTAATPMPTPTSS 947          

HSP 4 Score: 107.071 bits (266), Expect = 1.672e-22
Identity = 77/292 (26.37%), Postives = 141/292 (48.29%), Query Frame = 1
Query: 3832 SLYVFSVDNQ----FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            S +VFS        FR+  + IV+ K F+  ++  I +N  ++ +E    P +  +   L +TN++FT IF  EM   +L+ GL      Y+K+ +NI D  +V IS+ ++        ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F   F ILG+ LF  KF    G  L+   T  D            + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  630 SKFVFSKAGHAWKNFRETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQ--VLEITNFVFTSIFVLEMGFMLLAFGLW----GYIKNPYNIFDSVVVIISVWEIS-----AQADGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKF----GLRLENGDTIPD------------RKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SPWAALYFVALMTFGNYVLFNLLVAILVEGFQ 881          

HSP 5 Score: 87.0409 bits (214), Expect = 2.275e-16
Identity = 71/228 (31.14%), Postives = 119/228 (52.19%), Query Frame = 1
Query: 4783 RAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            +A   W+N+   R T+ ++V SKYF+ AI   I +N  +M  E+H  P+ L   L++ N+ FTS FVLE    L+A GL  YI + +N  D ++VI+S+  +                + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  + F+ LG+ LFG     RLE  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  636 KAGHAWKNF---RETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQVLEITNFVFTSIFVLEMGFMLLAFGLWGYIKNPYNIFDSVVVIISVWEISAQADGG---------LSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFGLRLENGDTIP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 846          

HSP 6 Score: 81.2629 bits (199), Expect = 1.155e-14
Identity = 61/223 (27.35%), Postives = 116/223 (52.02%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVI-----ELCQQGG----TSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT-----SSWAALYFIALMTFGNYVL 2826
            I + + IN L+M +E++ Q   L + L+  N VF+ +F +E +LKL+  GI  + K+ +N  D  IV L ++     EL  +G      + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   CT  N      R   F++   + +T+F++ T ++W+ ++ + +        + +A L +++   F  +VL
Sbjct: 1488 ITLIVGINILTMSVEHYNQQQYLKEALKDCNYVFTFIFFVEALLKLVAFGIHRFFKDKWNQLDVAIVALSILGIVLEELNMKGALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLKCTDINPCEGLSRHATFENFGMAFLTLFRVSTGDNWSGIMKDTLRECQPDDRNCFAYLAWVSPFYFVTFVL 1710          

HSP 7 Score: 76.6406 bits (187), Expect = 3.066e-13
Identity = 77/312 (24.68%), Postives = 151/312 (48.40%), Query Frame = 1
Query: 2140 YLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTC--------DRK-NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQ 2958
            + S CQ+ I   +    F   +L  I +N +++ +E    +P  L +  L  SN +F+++FLIEM +K++  G       Y++  +N+ DG++V + ++++       G       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  +F ILG+ LF  KF   E        NK  C        +RK NFD+L  +++++F +  ++ W  ++Y+G+               W  +YFI+ +   ++ + N+ V ++VE F   R D+++     R+  +++  + K+ R MQ
Sbjct: 1154 FRSCCQRVISHKL----FDHVVLFFIFLNCITIAMERPDIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVSIAYVGENKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCTFFIVFGILGVQLFKGKFYICEGFDTRNVTNKSDCLQANYRWTNRKYNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRRAMQ 1461          

HSP 8 Score: 63.5438 bits (153), Expect = 3.077e-9
Identity = 54/156 (34.62%), Postives = 86/156 (55.13%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M +P   +   S+    L V +      F  EM+IK+++ G+  G + YL   WN +D  +V   +L+  +    G +  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++ +C   F IFGI+GVQL+ G
Sbjct:    1 MMVILLNCVTLGMFQPCEDVDCLSERCKVLQVLDDCIFAFFAVEMVIKMMAFGVF-GSKCYLADRWNQLDFVIVMAGMLEYSLD---GQNASLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLFLCFFVFFIFGIVGVQLWAG 148          

HSP 9 Score: 58.5362 bits (140), Expect = 1.105e-7
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPS 666
             ++F      R  C ++I+   F+ + +  I +NCIT++M +P  D    + +   + A +YI    F  EM IK++A+GF  G   Y   +WN LD  +V V  +++L S
Sbjct: 1145 LYLFDPESWFRSCCQRVISHKLFDHVVLFFIFLNCITIAMERP--DIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVS 1253          

HSP 10 Score: 53.9138 bits (128), Expect = 2.394e-6
Identity = 32/110 (29.09%), Postives = 59/110 (53.64%), Query Frame = 1
Query: 4411 AVKTKNDCLKYSQ-------NEWKNQK--YNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            A  + N C+ ++Q        E+   K   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  227 AGASVNGCVNWNQYYNVCHAGEFNPHKGAVNFDNIGYASIAIFQVITLEGWVDIMYFVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 326          

HSP 11 Score: 52.7582 bits (125), Expect = 5.016e-6
Identity = 32/114 (28.07%), Postives = 58/114 (50.88%), Query Frame = 1
Query: 1099 TDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETE 1410
            T N+  K  C+  N  +T+ +           NFDN+  A +++F +   + WV+IMY   DA          H+ W  IYF++ ++I SFF++N+ + V+   F + ++ + E
Sbjct: 1335 TRNVTNKSDCLQANYRWTNRK----------YNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEE 1438          

HSP 12 Score: 52.373 bits (124), Expect = 7.572e-6
Identity = 23/78 (29.49%), Postives = 44/78 (56.41%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E  L+   R
Sbjct:  257 NFDNIGYASIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQR 334          

HSP 13 Score: 52.373 bits (124), Expect = 7.897e-6
Identity = 23/75 (30.67%), Postives = 44/75 (58.67%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQF---SETKRRETE 1410
            NFD++ +A V +FQ+++ E W  ++Y    + S W  +YFVAL+  G++ + NL + ++   F    +  R E++
Sbjct:  818 NFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSESD 892          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1ha (voltage-dependent T-type calcium channel subunit alpha-1H [Source:NCBI gene;Acc:101161560])

HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0
Identity = 393/617 (63.70%), Postives = 482/617 (78.12%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQ---RNC-CVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEE 5667
            SLY+F  ++ FR  CQ ++  K FD++VLFFI LNCIT+AMERP I PHS ERLFL+ +NYIFT+IF  EM IKV++ G   G Q YL+  WNI+DG LVF+SLLD+ ++I     N+I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICCTFFI+FGILGVQLFKGKFY C+G   + V  K+DCL+ +   W N+KYNFDNL +ALM+LFVLS KDGWV IMY G+DAVGV+ QP  NHN W LIYFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+EE   R  KR + ++KKR+      Y+ +YS  R  I+ + T+ Y DL I  ++G+N++TM+ E +   Q LK ALK  NY FT  F +EALLKLVA G+ R+  DKWNQLD+ IV LSI G+V++E+     +PINPTI+R+MRVLRIARVLKLLKMA G+RALLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+L+C+DINPC GL RHA FENFGMAFLTLFR++TGDNW+GIMKDTLRE       CQ   RNC    A ++P YFV FVL+AQFVLVNVVVAVLMKHLEES+K   +D E++ E+  +++ E
Sbjct: 1092 SLYLFDPESWFRSCCQRVISHKLFDHVVLFFIFLNCITIAMERPDIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVSIAYVGENKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCTFFIVFGILGVQLFKGKFYICEGFDTRNVTNKSDCLQ-ANYRWTNRKYNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRRAMQRPYYADYSPVRLYIHRLCTNHYLDLFITLIVGINILTMSVEHYNQQQYLKEALKDCNYVFTFIFFVEALLKLVAFGIHRFFKDKWNQLDVAIVALSILGIVLEELNMKGALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLKCTDINPCEGLSRHATFENFGMAFLTLFRVSTGDNWSGIMKDTLRE-------CQPDDRNCFAYLAWVSPFYFVTFVLIAQFVLVNVVVAVLMKHLEESNKEAKEDAEMDAEIALEMERE 1700          

HSP 2 Score: 335.109 bits (858), Expect = 4.629e-92
Identity = 204/383 (53.26%), Postives = 267/383 (69.71%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFT--DNLP--TKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLY 1554
            M +I +NC+TL M+QPC D +C + +C+++Q  D  IFAFF  EMVIK++A G  G   Y  D WN+LD  IV+ G +E     G N  L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVL LCFFVFFIFGI+GVQ+W G+LRNRC+L  +    +   +L  +Y           D  FICST + NG+L C D+PPS       SL   +Q +      D++   + + CVNWNQYY  C  GE NP++GA+NFDNIG+A +AIFQVI++E WVDIMY+V DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE   M  +R R+ SS+ +  S  EP +CY EI++ I+ +  K+ RR+ ++Y
Sbjct:    1 MMVILLNCVTLGMFQPCEDVDCLSERCKVLQVLDDCIFAFFAVEMVIKMMAFGVFGSKCYLADRWNQLDFVIVMAGMLE-YSLDGQNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLFLCFFVFFIFGIVGVQLWAGLLRNRCFLGEDTKVLY-NQSLKPYYVDE-----EGEDNPFICSTPRENGMLHCYDVPPSTEDGVECSLTASQQSSVLIGSFDSVAGASVNGCVNWNQYYNVCHAGEFNPHKGAVNFDNIGYASIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSSDSTFSSYTEPGSCYEEILRYISHLYRKLRRRLQRIY 376          

HSP 3 Score: 201.83 bits (512), Expect = 2.959e-51
Identity = 112/188 (59.57%), Postives = 144/188 (76.60%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF-CTIENNKRTCDRKNFDSLLWSIVTVFQ 2715
            ++ ++T+V    F + I+ AILINT SMGIEYH+QP  LTQ LE +N VF+ +F++EM   L+  G+ GYIKN +N+FD ++V++ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIF FSILGM LFGCKF   +EN     DRKNFDSLLW+IVTVFQ
Sbjct:  645 RETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQVLEITNFVFTSIFVLEMGFMLLAFGLWGYIKNPYNIFDSVVVIISVWEISAQ-ADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFGLRLENGDTIPDRKNFDSLLWAIVTVFQ 831          

HSP 4 Score: 81.2629 bits (199), Expect = 1.140e-14
Identity = 61/223 (27.35%), Postives = 115/223 (51.57%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVI-----ELCQQGG----TSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSS-----WAALYFIALMTFGNYVL 2826
            I + + IN L+M +E++ Q   L + L+  N VF+ +F +E +LKL+  GI  + K+ +N  D  IV L ++     EL  +G      + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   CT  N      R   F++   + +T+F++ T ++W+ ++ + +          +A L +++   F  +VL
Sbjct: 1436 ITLIVGINILTMSVEHYNQQQYLKEALKDCNYVFTFIFFVEALLKLVAFGIHRFFKDKWNQLDVAIVALSILGIVLEELNMKGALPINPTIIRIMRVLRIARVLKLLKMAKGMRALLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLKCTDINPCEGLSRHATFENFGMAFLTLFRVSTGDNWSGIMKDTLRECQPDDRNCFAYLAWVSPFYFVTFVL 1658          

HSP 5 Score: 79.7221 bits (195), Expect = 3.373e-14
Identity = 65/231 (28.14%), Postives = 111/231 (48.05%), Query Frame = 1
Query: 3832 SLYVFSVDNQ----FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALF 4512
            S +VFS        FR+  + IV+ K F+  ++  I +N  ++ +E    P +  +   L +TN++FT IF  EM   +L+ GL      Y+K+ +NI D  +V IS+ ++        ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F   F ILG+ LF  KF    G  L+   T  D            + NFD+L  A++ +F
Sbjct:  630 SKFVFSKAGHAWKNFRETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQ--VLEITNFVFTSIFVLEMGFMLLAFGLW----GYIKNPYNIFDSVVVIISVWEIS-----AQADGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKF----GLRLENGDTIPD------------RKNFDSLLWAIVTVF 830          

HSP 6 Score: 76.6406 bits (187), Expect = 3.406e-13
Identity = 77/312 (24.68%), Postives = 151/312 (48.40%), Query Frame = 1
Query: 2140 YLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTC--------DRK-NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQ 2958
            + S CQ+ I   +    F   +L  I +N +++ +E    +P  L +  L  SN +F+++FLIEM +K++  G       Y++  +N+ DG++V + ++++       G       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  +F ILG+ LF  KF   E        NK  C        +RK NFD+L  +++++F +  ++ W  ++Y+G+               W  +YFI+ +   ++ + N+ V ++VE F   R D+++     R+  +++  + K+ R MQ
Sbjct: 1102 FRSCCQRVISHKL----FDHVVLFFIFLNCITIAMERPDIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVSIAYVGENKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCTFFIVFGILGVQLFKGKFYICEGFDTRNVTNKSDCLQANYRWTNRKYNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRRAMQ 1409          

HSP 7 Score: 76.2554 bits (186), Expect = 4.347e-13
Identity = 67/213 (31.46%), Postives = 109/213 (51.17%), Query Frame = 1
Query: 4783 RAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFR 5406
            +A   W+N+   R T+ ++V SKYF+ AI   I +N  +M  E+H  P+ L   L++ N+ FTS FVLE    L+A GL  YI + +N  D ++VI+S+  +                + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  + F+ LG+ LFG     RLE  D  P        +F++   A +T+F+
Sbjct:  636 KAGHAWKNF---RETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQVLEITNFVFTSIFVLEMGFMLLAFGLWGYIKNPYNIFDSVVVIISVWEISAQADGG---------LSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFGLRLENGDTIP-----DRKNFDSLLWAIVTVFQ 831          

HSP 8 Score: 63.5438 bits (153), Expect = 3.336e-9
Identity = 54/156 (34.62%), Postives = 86/156 (55.13%), Query Frame = 1
Query: 3916 LFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            +  I LNC+TL M +P   +   S+    L V +      F  EM+IK+++ G+  G + YL   WN +D  +V   +L+  +    G +  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++ +C   F IFGI+GVQL+ G
Sbjct:    1 MMVILLNCVTLGMFQPCEDVDCLSERCKVLQVLDDCIFAFFAVEMVIKMMAFGVF-GSKCYLADRWNQLDFVIVMAGMLEYSLD---GQNASLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLFLCFFVFFIFGIVGVQLWAG 148          

HSP 9 Score: 58.151 bits (139), Expect = 1.149e-7
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPS 666
             ++F      R  C ++I+   F+ + +  I +NCIT++M +P  D    + +   + A +YI    F  EM IK++A+GF  G   Y   +WN LD  +V V  +++L S
Sbjct: 1093 LYLFDPESWFRSCCQRVISHKLFDHVVLFFIFLNCITIAMERP--DIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVS 1201          

HSP 10 Score: 53.9138 bits (128), Expect = 2.470e-6
Identity = 32/110 (29.09%), Postives = 59/110 (53.64%), Query Frame = 1
Query: 4411 AVKTKNDCLKYSQ-------NEWKNQK--YNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            A  + N C+ ++Q        E+   K   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  227 AGASVNGCVNWNQYYNVCHAGEFNPHKGAVNFDNIGYASIAIFQVITLEGWVDIMYFVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 326          

HSP 11 Score: 53.1434 bits (126), Expect = 4.838e-6
Identity = 32/114 (28.07%), Postives = 58/114 (50.88%), Query Frame = 1
Query: 1099 TDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETE 1410
            T N+  K  C+  N  +T+ +           NFDN+  A +++F +   + WV+IMY   DA          H+ W  IYF++ ++I SFF++N+ + V+   F + ++ + E
Sbjct: 1283 TRNVTNKSDCLQANYRWTNRK----------YNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEE 1386          

HSP 12 Score: 52.373 bits (124), Expect = 6.886e-6
Identity = 23/78 (29.49%), Postives = 44/78 (56.41%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E  L+   R
Sbjct:  257 NFDNIGYASIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQR 334          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1hb (calcium voltage-gated channel subunit alpha1 H [Source:NCBI gene;Acc:101160713])

HSP 1 Score: 639.032 bits (1647), Expect = 0.000e+0
Identity = 382/595 (64.20%), Postives = 480/595 (80.67%), Query Frame = 1
Query: 3832 SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNC-CVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHK 5610
            SLY+F+ DN+FR  C  ++  K FD++VL FI LNCIT+A+ERP I P S ER+FL+V+NYIFT+IF GEM+IKV++ GL  G+  YL+S WNI+DG LVF+SL+D+ ++I     NRI GILRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICC FFI+FGILGVQLFKGKF++C+G H++ +  + +CL+ + + W  +KYNFDNLGQALM+LFVLS KDGWV IMY G+DAVGV+ QP  N+N W L++FISFLL+V+FFVLNMFVGVVVENFHKC++ QE+EE   R  KR +RLEK+R+      Y+ +YS  RR+I+++ TS Y DL I  +I  N++TM+ E +  PQ L+  LK  NY FT  FV+EA+LKL+A GL R+  ++WNQLD+ IV+LSI G+ ++E++ S  +PINPTI+R+MRVLRI RVLKLLKMA+G+R+LLDTV+QALPQVGNLGLLF LLF+I+AALGVELFG+LECS+ NPC GL RHA F+NFGMAFLTLFR++TGDNWNGIMKDTLRE C   E   R+C     +I+P+YFV FVL AQFVLVNVVVAVLMKHLEES+K
Sbjct: 1128 SLYLFAPDNRFRLWCLRVIGHKMFDHIVLLFIFLNCITIALERPDISPRSMERVFLSVSNYIFTVIFVGEMMIKVVAMGLYFGEGVYLQSSWNILDGVLVFVSLVDILVSIASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFFHCEGLHVRNITNRTECLQ-AGHRWVRRKYNFDNLGQALMSLFVLSCKDGWVSIMYDGLDAVGVDQQPIRNNNPWMLLFFISFLLIVSFFVLNMFVGVVVENFHKCRQQQEEEEARIREEKRQKRLEKRRRRALEKPYYADYSPLRRSIHTLCTSHYLDLFITVIIFTNLLTMSMEHYNQPQYLEEILKYCNYVFTLVFVIEAILKLIAFGLRRFFKERWNQLDLAIVLLSIMGIALEEIDLSASLPINPTIIRIMRVLRITRVLKLLKMATGMRSLLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSEENPCEGLSRHATFDNFGMAFLTLFRVSTGDNWNGIMKDTLRE-CRPHE---RHCLSYLPLISPVYFVTFVLTAQFVLVNVVVAVLMKHLEESNK 1717          

HSP 2 Score: 375.17 bits (962), Expect = 2.096e-104
Identity = 223/424 (52.59%), Postives = 294/424 (69.34%), Query Frame = 1
Query:  307 LIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIER--RRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIR 1572
            L Y      V     Q   PR WCL+ +  PWFE++S+  I +NC+TL M+QPCG          ++QA D  IFAFF  EMV+K++A+G +G  GY GDTWNRLD FIVIVG +E     G+N+ L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVL LCFFVFFIFGI+GVQ+W G+LRNRC++  +   R+    L+ +Y+ + T      D  FICST + NG+L+CSD+P   +  ++ +L   +  +        ++ SCVNW Q+Y  C+ GE NP++GAINFDNIG+AW+AIFQVI++E WVDIMYYV DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE   M  E+  R+ + S  +  S  +P +CY EII+ +  +  K +RRI + Y+QI  R
Sbjct:   58 LPYPSLAPVVLLALTQTSPPRSWCLRAVCHPWFERVSILAILLNCVTLGMFQPCGHTP------SVLQALDDGIFAFFAGEMVVKMVALGVIGQKGYLGDTWNRLDFFIVIVGMLE-YSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLALCFFVFFIFGIVGVQLWAGLLRNRCFIEEDVKMRYNITFLNDYYKPDGT-----DDHPFICSTARENGMLRCSDVPRRRVGRTHCNLDADEAFSETGLKGDESE-SCVNWYQFYNQCRAGELNPHKGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFIFLIIVGSFFMINLCLVVIATQFSETKQREHALMKSEQRARQLHQSASTLASDSQPGSCYEEIIRYLAHLGRKAWRRISRCYSQIWRR 468          

HSP 3 Score: 290.812 bits (743), Expect = 2.209e-78
Identity = 181/311 (58.20%), Postives = 222/311 (71.38%), Query Frame = 1
Query: 2161 KIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTC-DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSE------RLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATP 3072
            K+K +V+   F RGI+IAILINTLSMGIEYH+QP +LT  LE SN+VF+ LF +EM+LKL+  G+ GYIKN +N FD IIV++ V E+  Q    GLS+LRTFRLLR+LKLVRF+PALR+QL V+++T+DNVATF  LL+LFIFIFSILGM LFGCKF   +++  T  DRKNFDSLLW+ VTVFQILTQEDWN VLYNGM  TS  AALYF+ALMTFGNYVLFNLLVAILVEGF +E        D+ +L   Y    K +     +L+I     + ++L  N +   +           PVI HTAATP
Sbjct:  674 KLKLIVSSRYFNRGIMIAILINTLSMGIEYHEQPQELTDILEISNMVFTSLFSLEMLLKLLALGLFGYIKNPYNGFDSIIVIISVWEIVGQA-EGGLSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGLRQDSGDTLPDRKNFDSLLWATVTVFQILTQEDWNAVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSEADDDRLSLCYHDEEKLKELYAAELKI-----QAMMLSPNGLLNPKASMPPPPPPPLPVITHTAATP 978          

HSP 4 Score: 92.8189 bits (229), Expect = 4.082e-18
Identity = 71/259 (27.41%), Postives = 129/259 (49.81%), Query Frame = 1
Query: 2104 FNKNQNKTKRKNYL---SICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQG---------GTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM--TRTSSWAALYFIALMTFGNYVLFNL 2835
              K + +   K Y    S  ++ I TL         I + I  N L+M +E++ QP  L + L++ N VF+++F+IE ILKLI  G+  + K  +N  D  IV+L ++ +  +            + + I+R  R+ R+LKL++    +R  L  +++ +  V     L +L  FI++ LG+ LFG   C+ EN      R   FD+   + +T+F++ T ++WN ++ + +   R      L ++ L++   +V F L
Sbjct: 1436 LEKRRRRALEKPYYADYSPLRRSIHTLCTSHYLDLFITVIIFTNLLTMSMEHYNQPQYLEEILKYCNYVFTLVFVIEAILKLIAFGLRRFFKERWNQLDLAIVLLSIMGIALEEIDLSASLPINPTIIRIMRVLRITRVLKLLKMATGMRSLLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECSEENPCEGLSRHATFDNFGMAFLTLFRVSTGDNWNGIMKDTLRECRPHERHCLSYLPLISPVYFVTFVL 1694          

HSP 5 Score: 86.2705 bits (212), Expect = 3.735e-16
Identity = 89/390 (22.82%), Postives = 170/390 (43.59%), Query Frame = 1
Query: 3550 MAYKNKINQLTIPF---SNRYSLSLDSEGKSN-----YQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKC-CPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            M   N++ QL +     +   +L L  EG        Y    + L   E   E P++ +   QNS   N+    +        C       +P+    +R +  L         R K + IV  + F+  ++  I +N +++ +E    P    +   L ++N +FT +F+ EM++K+L+ GL      Y+K+ +N  D  +V IS+ +     ++G +     +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F  IF ILG+ LF  KF                 L+    +    + NFD+L  A + +F + +++ W  ++Y G+ +           +  A +YF++ +    + + N+ V ++VE F
Sbjct:  567 METINRLPQLVLEHGGSAGHPALQLPGEGSGEASSCPYCTYYSQLSGSENLHEHPEEQHHRYQNSRLGNHPCHGNSPCQSRSPCYGNAQVLEPRKKLWERCWAGL---------RIKLKLIVSSRYFNRGIMIAILINTLSMGIEYHEQPQELTD--ILEISNMVFTSLFSLEMLLKLLALGLF----GYIKNPYNGFDSIIVIISVWE-----IVGQAEGGLSVLRTFRLLRVLKLVRFL---PALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKF----------------GLRQDSGDTLPDRKNFDSLLWATVTVFQILTQEDWNAVLYNGMAST----------SPLAALYFVALMTFGNYVLFNLLVAILVEGF 907          

HSP 6 Score: 85.5001 bits (210), Expect = 6.032e-16
Identity = 61/171 (35.67%), Postives = 98/171 (57.31%), Query Frame = 1
Query: 3865 RQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C   V    F+ + +  I LNC+TL M +P    H+   +   + + IF   F GEM++K+++ G+ IG + YL   WN +D F+V + +L+  +    GH+  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++ +C   F IFGI+GVQL+ G
Sbjct:   78 RSWCLRAVCHPWFERVSILAILLNCVTLGMFQPC--GHTPS-VLQALDDGIFAF-FAGEMVVKMVALGV-IGQKGYLGDTWNRLDFFIVIVGMLEYSLD---GHNVSLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLALCFFVFFIFGIVGVQLWAG 236          

HSP 7 Score: 78.1814 bits (191), Expect = 1.236e-13
Identity = 73/224 (32.59%), Postives = 121/224 (54.02%), Query Frame = 1
Query: 4798 WEN-YSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            WE  ++  R  +  +V+S+YF+  I   I +N ++M  E+H  PQ L   L++ N  FTS F LE LLKL+ALGL  YI + +N  D +IVI+S+  +V  + E          + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  +IF+ LG+ LFG     R +  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  664 WERCWAGLRIKLKLIVSSRYFNRGIMIAILINTLSMGIEYHEQPQELTDILEISNMVFTSLFSLEMLLKLLALGLFGYIKNPYNGFDSIIVIISVWEIV-GQAEGG--------LSVLRTFRLLRVLKLVRFLPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGLRQDSGDTLP-----DRKNFDSLLWATVTVFQILTQEDWNAVLYNGM 873          

HSP 8 Score: 69.3218 bits (168), Expect = 4.919e-11
Identity = 67/297 (22.56%), Postives = 148/297 (49.83%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLI------GEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTC---------DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQ 2958
            F   +L+ I +N +++ +E     P  + +  L  SN +F+++F+ EM++K++      GEG+  Y+++ +N+ DG++V + ++++     +SG       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  +F ILG+ LF  KF   E        N+  C          + NFD+L  +++++F +  ++ W +++Y+G+               W  L+FI+ +   ++ + N+ V ++VE F   R  +++     R+  +++R   ++ R ++
Sbjct: 1151 FDHIVLLFIFLNCITIALERPDISPRSMERVFLSVSNYIFTVIFVGEMMIKVVAMGLYFGEGV--YLQSSWNILDGVLVFVSLVDILVSIASSGGNRILGILRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFFHCEGLHVRNITNRTECLQAGHRWVRRKYNFDNLGQALMSLFVLSCKDGWVSIMYDGLDAVGVDQQPIRNNNPWMLLFFISFLLIVSFFVLNMFVGVVVENFHKCRQQQEEEEARIREEKRQKRLEKRRRRALE 1445          

HSP 9 Score: 67.781 bits (164), Expect = 1.419e-10
Identity = 36/117 (30.77%), Postives = 65/117 (55.56%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMG-FLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIG 684
             ++F  +   R WCL++I    F+ + +  I +NCIT+++ +P  D +  + +   +   +YI    F  EM+IK++AMG + G+  Y   +WN LD  +V V  ++IL S  ++ G
Sbjct: 1129 LYLFAPDNRFRLWCLRVIGHKMFDHIVLLFIFLNCITIALERP--DISPRSMERVFLSVSNYIFTVIFVGEMMIKVVAMGLYFGEGVYLQSSWNILDGVLVFVSLVDILVSIASSGG 1243          

HSP 10 Score: 60.8474 bits (146), Expect = 2.145e-8
Identity = 36/124 (29.03%), Postives = 74/124 (59.68%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTL--EFSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            F+R  ++AIL+N +++G+    QP   T ++     + +F+  F  EM++K++  G++G   Y+ + +N  D  IV++G++E    G    LS +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:   90 FERVSILAILLNCVTLGMF---QPCGHTPSVLQALDDGIFA-FFAGEMVVKMVALGVIGQKGYLGDTWNRLDFFIVIVGMLEYSLDGHNVSLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTL 209          

HSP 11 Score: 54.6842 bits (130), Expect = 1.508e-6
Identity = 30/96 (31.25%), Postives = 52/96 (54.17%), Query Frame = 1
Query: 4426 NDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            N C     N  K    NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  326 NQCRAGELNPHKG-AINFDNIGYAWIAIFQVITLEGWVDIMYYVMDA----------HSFYNFIYFIFLIIVGSFFMINLCLVVIATQFSETKQRE 410          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Medaka
Match: cacna1ha (voltage-dependent T-type calcium channel subunit alpha-1H [Source:NCBI gene;Acc:101161560])

HSP 1 Score: 577.015 bits (1486), Expect = 2.220e-171
Identity = 385/669 (57.55%), Postives = 477/669 (71.30%), Query Frame = 1
Query: 3670 EKALEEPDDSN--SESQNSSRMNNVEDPDE-EDMEAMICLSKCCPQP--KGIFAKRYYH-SLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXX--------XXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVE-SNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRL-ECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQ---RNC-CVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMM 5616
            +K+LE+P  S+   E  + + M     PDE  D +  I L     +   K  + K +   SLY+F  ++ FR  CQ ++  K FD++VLFFI LNCIT+AMERP I PHS ERLFL+ +NYIFT+IF  EM IKV++ G   G Q YL+  WNI+DG LVF+SLLD+ ++I     N+I G+LRV RLLRTLRPLRVISRAPGLKLVV+TL++SLRPIGNIVLICCTFFI+FGILGVQLFKGKFY C+G   + V  K+DCL+ +   W N+KYNFDNL +ALM+LFVLS KDGWV IMY G+DAVGV+ QP  NHN W LIYFISFLL+V+FFVLNMFVGVVVENFHKC+++QE+EE              KR  +       ++ P Y+ +YS  R  I+ + T+ Y DL I  ++G+N++TM+ E +   Q LK ALK  NY FT  F +EALLKLVA G+ R+  DKWNQLD+ IV LSI G+V++E+     +PINPTI+R+MRVLRIAR   L +M   I +          QVGNLGLLF LLF+I+AALGVELFG+L EC+DINPC GL RHA FENFGMAFLTLFR++TGDNW+GIMKDTLRE       CQ   RNC    A ++P YFV FVL+AQFVLVNVVVAVLMKHLEES+K++
Sbjct:  955 QKSLEQPQVSSMVGEHSDCNGMTLTCSPDENADAKDDISLEDDFDEDNLKPRWCKEHEDCSLYLFDPESWFRSCCQRVISHKLFDHVVLFFIFLNCITIAMERPDIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVSIAYVGENKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCTFFIVFGILGVQLFKGKFYICEGFDTRNVTNKSDCLQ-ANYRWTNRKYNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRSKENGGAGAMQRP-YYADYSPVRLYIHRLCTNHYLDLFITLIVGINILTMSVEHYNQQQYLKEALKDCNYVFTFIFFVEALLKLVAFGIHRFFKDKWNQLDVAIVALSILGIVLEELNMKGALPINPTIIRIMRVLRIARGNFLDRMELNIASFCF-------QVGNLGLLFMLLFFIYAALGVELFGKLIECTDINPCEGLSRHATFENFGMAFLTLFRVSTGDNWSGIMKDTLRE-------CQPDDRNCFAYLAWVSPFYFVTFVLIAQFVLVNVVVAVLMKHLEESNKVL 1607          

HSP 2 Score: 348.977 bits (894), Expect = 1.689e-96
Identity = 220/467 (47.11%), Postives = 295/467 (63.17%), Query Frame = 1
Query:  289 INPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWP----------WFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKH--SCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNY----QNKLKRQRK-HCNV 1638
            I+  E   +       F    Q   PR WC++I+  P          W   +SM +I +NC+TL M+QPC D +C + +C+++Q  D  IFAFF  EMVIK++A G  G   Y  D WN+LD  IV+ G +E     G N  L+AIRT+RVLRPLRAINR+PSMRILV LLLDTLPMLGNVL LCFFVFFIFGI+GVQ+W G+LRNRC+L  +     P       Y ++        D  FICST + NG+L C D+PPS    + + ++           +L   H   CVNWNQYY  C  GE NP++GA+NFDNIG+A +AIFQVI++E WVDIMY+V DAHSF+++IYF+ LII+GSFFMINLCLVVIA+QFSETK+RE   M  +R R+ SS+ +  S  EP +CY EI++ I+ +  K+ RR+ ++Y +    HS      +     N +++ R+ HC++
Sbjct:   54 ISCEEEQPFPALAPKAFFCLKQTTRPRYWCIRIVCNPYPFTTLEKYLWRVYISMMVILLNCVTLGMFQPCEDVDCLSERCKVLQVLDDCIFAFFAVEMVIKMMAFGVFGSKCYLADRWNQLDFVIVMAGMLE-YSLDGQNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLFLCFFVFFIFGIVGVQLWAGLLRNRCFLGEDTKSLKP-------YYVDE----EGEDNPFICSTPRENGMLHCYDVPPS----TEDGVEC----------SLTASHVNGCVNWNQYYNVCHAGEFNPHKGAVNFDNIGYASIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQRARYMSSDSTFSSYTEPGSCYEEILRYISHLYRKLRRRLQRIYYRW---HSGPGAAFWLRPIHNLMQQHRQEHCHL 491          

HSP 3 Score: 310.071 bits (793), Expect = 1.883e-84
Identity = 176/314 (56.05%), Postives = 217/314 (69.11%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF-CTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSS 3093
            ++ ++T+V    F + I+ AILINT SMGIEYH+QP  LTQ LE +N VF+ +F++EM   L+  G+ GYIKN +N+FD ++V++ V E+  Q    GLS+LRTFRLLR+LKLVRFMPALR+QL V+++T+DNVATF  LL+LFIF FSILGM LFGCKF   +EN     DRKNFDSLLW+IVTVFQILTQEDWN VLYNGM  TS WAALYF+ALMTFGNYVLFNLLVAILVEGF +E                  R  +          + L +  N    D  G     ++ PP+I+ TAATPMPTP SS
Sbjct:  574 RETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQVLEITNFVFTSIFVLEMGFMLLAFGLWGYIKNPYNIFDSVVVIISVWEISAQA-DGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFGLRLENGDTIPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAE-----------------VRMTHIFFSHFFFAFKALEMTANG-HIDACG-----TTAPPIIMRTAATPMPTPTSS 863          

HSP 4 Score: 105.531 bits (262), Expect = 5.243e-22
Identity = 77/292 (26.37%), Postives = 141/292 (48.29%), Query Frame = 1
Query: 3832 SLYVFSVDNQ----FRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFH 4695
            S +VFS        FR+  + IV+ K F+  ++  I +N  ++ +E    P +  +   L +TN++FT IF  EM   +L+ GL      Y+K+ +NI D  +V IS+ +     +   ++    +LR FRLLR L+ +R +   P L+  +  L+ ++  +    ++   F   F ILG+ LF  KF    G  L+   T  D            + NFD+L  A++ +F + +++ W  ++Y G+ +           + WA +YF++ +    + + N+ V ++VE F 
Sbjct:  559 SKFVFSKAGHAWKNFRETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQ--VLEITNFVFTSIFVLEMGFMLLAFGLW----GYIKNPYNIFDSVVVIISVWE-----ISAQADGGLSVLRTFRLLRVLKLVRFM---PALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKF----GLRLENGDTIPD------------RKNFDSLLWAIVTVFQILTQEDWNVVLYNGMAST----------SPWAALYFVALMTFGNYVLFNLLVAILVEGFQ 810          

HSP 5 Score: 93.2041 bits (230), Expect = 3.295e-18
Identity = 93/326 (28.53%), Postives = 152/326 (46.63%), Query Frame = 1
Query: 3907 YLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG---------------KFYY-----------CDGP------HLQAVKTK--------------NDCLKYSQ-------NEWKNQK--YNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            Y+ +  I LNC+TL M +P   +   S+    L V +      F  EM+IK+++ G+  G + YL   WN +D  +V   +L+  +    G +  +  I    R +R LRPLR I+R P ++++V  LL +L  +GN++ +C   F IFGI+GVQL+ G               K YY           C  P      H   V                 N C+ ++Q        E+   K   NFDN+G A +A+F + + +GWV IMY  +DA          H+ +  IYFI  +++ +FF++N+ + V+   F + ++ +
Sbjct:  104 YISMMVILLNCVTLGMFQPCEDVDCLSERCKVLQVLDDCIFAFFAVEMVIKMMAFGVF-GSKCYLADRWNQLDFVIVMAGMLEYSLD---GQNASLSAI----RTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLFLCFFVFFIFGIVGVQLWAGLLRNRCFLGEDTKSLKPYYVDEEGEDNPFICSTPRENGMLHCYDVPPSTEDGVECSLTASHVNGCVNWNQYYNVCHAGEFNPHKGAVNFDNIGYASIAIFQVITLEGWVDIMYFVMDA----------HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRE 411          

HSP 6 Score: 86.6557 bits (213), Expect = 2.576e-16
Identity = 71/228 (31.14%), Postives = 119/228 (52.19%), Query Frame = 1
Query: 4783 RAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFG-----RLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            +A   W+N+   R T+ ++V SKYF+ AI   I +N  +M  E+H  P+ L   L++ N+ FTS FVLE    L+A GL  YI + +N  D ++VI+S+  +                + V+R  R+ RVLKL++    +R  L  +++ +  V    +L  L  + F+ LG+ LFG     RLE  D  P        +F++   A +T+F+I T ++WN ++ + +
Sbjct:  565 KAGHAWKNF---RETLRTIVESKYFNQAIMTAILINTFSMGIEYHEQPEYLTQVLEITNFVFTSIFVLEMGFMLLAFGLWGYIKNPYNIFDSVVVIISVWEISAQADGG---------LSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFTFSILGMHLFGCKFGLRLENGDTIP-----DRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM 775          

HSP 7 Score: 73.1738 bits (178), Expect = 3.646e-12
Identity = 80/309 (25.89%), Postives = 149/309 (48.22%), Query Frame = 1
Query: 2140 YLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQ-QPVQLTQT-LEFSNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGVIELCQQGGTSG-------LSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIEN-------NKRTC--------DRK-NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLD----EQKLLHNYRQSL--KKRRA 2931
            + S CQ+ I   +    F   +L  I +N +++ +E    +P  L +  L  SN +F+++FLIEM +K++  G       Y++  +N+ DG++V + ++++       G       L +LR  R LR L+++   P L+  +  +I ++  +     +   F  +F ILG+ LF  KF   E        NK  C        +RK NFD+L  +++++F +  ++ W  ++Y+G+               W  +YFI+ +   ++ + N+ V ++VE F   R D    E +L  + RQ +  KKRR+
Sbjct: 1025 FRSCCQRVISHKL----FDHVVLFFIFLNCITIAMERPDIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVSIAYVGENKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLRPIGNIVLICCTFFIVFGILGVQLFKGKFYICEGFDTRNVTNKSDCLQANYRWTNRKYNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRS 1329          

HSP 8 Score: 59.6918 bits (143), Expect = 4.240e-8
Identity = 57/221 (25.79%), Postives = 106/221 (47.96%), Query Frame = 1
Query: 2203 ILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVI-----ELCQQGGTS-GLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF-CTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRT-----SSWAALYFIALMTFGNYVL 2826
            I + + IN L+M +E++ Q   L + L+  N VF+ +F +E +LKL+  GI  + K+ +N  D  IV L ++     EL  +G      +I+R  R+LRI +   F+  +   +      + N+   F LL          G+ LFG    CT  N      R   F++   + +T+F++ T ++W+ ++ + +        + +A L +++   F  +VL
Sbjct: 1366 ITLIVGINILTMSVEHYNQQQYLKEALKDCNYVFTFIFFVEALLKLVAFGIHRFFKDKWNQLDVAIVALSILGIVLEELNMKGALPINPTIIRIMRVLRIAR-GNFLDRMELNIASFCFQVGNLGLLFMLLFFIYAAL---GVELFGKLIECTDINPCEGLSRHATFENFGMAFLTLFRVSTGDNWSGIMKDTLRECQPDDRNCFAYLAWVSPFYFVTFVL 1582          

HSP 9 Score: 57.7658 bits (138), Expect = 1.816e-7
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  337 FHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGF-LGDLGYFGDTWNRLDMFIVIVGSIEILPS 666
             ++F      R  C ++I+   F+ + +  I +NCIT++M +P  D    + +   + A +YI    F  EM IK++A+GF  G   Y   +WN LD  +V V  +++L S
Sbjct: 1016 LYLFDPESWFRSCCQRVISHKLFDHVVLFFIFLNCITIAMERP--DIKPHSLERLFLSASNYIFTVIFLIEMAIKVVALGFCFGKQCYLQCSWNILDGLLVFVSLLDVLVS 1124          

HSP 10 Score: 52.7582 bits (125), Expect = 5.756e-6
Identity = 39/146 (26.71%), Postives = 69/146 (47.26%), Query Frame = 1
Query: 1099 TDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQF---------SETKRRETERM-WIERRRFNSSNCSSISKDEP 1476
            T N+  K  C+  N  +T+ +           NFDN+  A +++F +   + WV+IMY   DA          H+ W  IYF++ ++I SFF++N+ + V+   F          E + RE +R   I+++R +  N  + +   P
Sbjct: 1206 TRNVTNKSDCLQANYRWTNRK----------YNFDNLFKALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPVRNHNPWMLIYFISFLLIVSFFVLNMFVGVVVENFHKCRQDQEEEEARLREDKRQKMIDKKRRSKENGGAGAMQRP 1341          

HSP 11 Score: 51.9878 bits (123), Expect = 9.777e-6
Identity = 23/78 (29.49%), Postives = 44/78 (56.41%), Query Frame = 1
Query: 2674 NFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYR 2907
            NFD++ ++ + +FQ++T E W  ++Y  M   S +  +YFI L+  G++ + NL + ++   FS  +  E  L+   R
Sbjct:  342 NFDNIGYASIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRENALMREQR 419          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Medaka
Match: ENSORLT00000045672.1 (pep primary_assembly:ASM223467v1:1:25452484:25669127:1 gene:ENSORLG00000026938.1 transcript:ENSORLT00000045672.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 285.419 bits (729), Expect = 3.833e-85
Identity = 178/356 (50.00%), Postives = 230/356 (64.61%), Query Frame = 1
Query:  268 QETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPW-FEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIII 1332
            +E DG+       L Y      VF    Q   PR WC+ ++  P+      M +I +NC+TL MYQPC + +C + +CQ +QAFD  I+ FF  EM+IK++A+G  G   Y GDTWNRLD FIV+ G +E       N+ LTAIRT+RVLRPL+AINR+PSMRILV LLLDTLPMLGNVLLLCFFVFFIFGIIGVQ+W G+LRNRCY   N +     L+L  +Y  +        ++ FICS   +NGI+ C D+P           ++C        D +     CVNWNQYYT C  G +NP++ AINFDNI +AW+ IFQVI++E WV+IMYYV DAHSF+++IYF+ LII+
Sbjct:   31 REEDGSSDTSVLMLPYPELVPVVFFCLKQTTFPRSWCIHMVCSPYPLNCDGMMVILLNCVTLGMYQPCENIDCSSERCQTLQAFDAFIYIFFALEMIIKMVALGIFGRRSYLGDTWNRLDFFIVMAGMVEYSLDL-QNMNLTAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTL----LDLEEYYMPDED-----DERPFICSLPIDNGIMSCMDVPAR-----REGGRVCCLDK---DDVVSLAGLCVNWNQYYTRCHTGSRNPHKDAINFDNIAYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIV 368          

HSP 2 Score: 75.485 bits (184), Expect = 1.851e-13
Identity = 61/174 (35.06%), Postives = 97/174 (55.75%), Query Frame = 1
Query: 3865 RQKCQHIV-DKKSFDYLVLFFITLNCITLAMERP--SIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            R  C H+V      +   +  I LNC+TL M +P  +I   S+    L   +    I F  EMIIK+++ G+  G ++YL   WN +D F+V   +++   ++ L + N     L   R +R LRPL+ I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQL+ G
Sbjct:   64 RSWCIHMVCSPYPLNCDGMMVILLNCVTLGMYQPCENIDCSSERCQTLQAFDAFIYIFFALEMIIKMVALGIF-GRRSYLGDTWNRLDFFIVMAGMVEY--SLDLQNMN-----LTAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAG 229          

HSP 3 Score: 55.4546 bits (132), Expect = 4.050e-7
Identity = 34/121 (28.10%), Postives = 68/121 (56.20%), Query Frame = 1
Query: 2206 LIAILINTLSMGIEYHQQPVQLT----QTLEFSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            ++ IL+N +++G+    + +  +    QTL+  +    I F +EMI+K++  GI G   Y+ + +N  D  IV+ G++E         L+ +RT R+LR LK +  +P++R  + +++ T+
Sbjct:   82 MMVILLNCVTLGMYQPCENIDCSSERCQTLQAFDAFIYIFFALEMIIKMVALGIFGRRSYLGDTWNRLDFFIVMAGMVEYSLDLQNMNLTAIRTVRVLRPLKAINRVPSMRILVNLLLDTL 202          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000005760.1 (SMESG000005760.1)

HSP 1 Score: 3513.39 bits (9109), Expect = 0.000e+0
Identity = 1891/1897 (99.68%), Postives = 1894/1897 (99.84%), Query Frame = 1
Query:  418 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVXXXXXXXXXXXXXXXQSILSINKSFETTSSRNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFXXXXXXXXXXXXXQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINKS 6108
            MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKLYNQ+QIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDS SKHR+PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFSILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSSRNINTTILQEDASIMP MKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDD+NSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLKLVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKIN S
Sbjct:    1 MTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKLYNQMQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSSSKHRKPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFSILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSSRNINTTILQEDASIMPRMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDTNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLKLVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINVS 1897          

HSP 2 Score: 61.6178 bits (148), Expect = 8.196e-9
Identity = 51/196 (26.02%), Postives = 85/196 (43.37%), Query Frame = 1
Query:   79 RKKYSLDSFPSSVF-YEANEFLEIENEIECLDILPQDNRSRRETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            R   SLDS   S + ++ N  L  E  +E     P D  S  +    N S  N  +    +  +     +   P    I+   Y    ++F  +   R  C  I+    F+ L +  I +NCITL+M +P    +  + +   +   +YI    FT EM+IK+++ G L GD  Y    WN +D F+V +  +++ 
Sbjct: 1061 RYSLSLDSEGKSNYQFKMNYLLAREKALE----EPDDTNSESQ----NSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLF 1246          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000005760.1 (SMESG000005760.1)

HSP 1 Score: 3492.21 bits (9054), Expect = 0.000e+0
Identity = 1882/1902 (98.95%), Postives = 1890/1902 (99.37%), Query Frame = 1
Query:  454 MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVXXXXXXXXXXXXXXXQSILSINKSFETTSSRNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFXXXXXXXXXXXXXQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINKSQQSTTEL---RLSEKRK 6150
            MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKLYNQ+QIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDS SKHR+PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFSILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSSRNINTTILQEDASIMP MKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDD+NSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLKLVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKIN +  +   L   ++ +KRK
Sbjct:    1 MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKLYNQMQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSSSKHRKPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFSILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSSRNINTTILQEDASIMPRMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDTNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLKLVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINSTLNACWRLLLPQMVQKRK 1902          

HSP 2 Score: 61.6178 bits (148), Expect = 8.228e-9
Identity = 51/196 (26.02%), Postives = 85/196 (43.37%), Query Frame = 1
Query:   79 RKKYSLDSFPSSVF-YEANEFLEIENEIECLDILPQDNRSRRETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            R   SLDS   S + ++ N  L  E  +E     P D  S  +    N S  N  +    +  +     +   P    I+   Y    ++F  +   R  C  I+    F+ L +  I +NCITL+M +P    +  + +   +   +YI    FT EM+IK+++ G L GD  Y    WN +D F+V +  +++ 
Sbjct: 1049 RYSLSLDSEGKSNYQFKMNYLLAREKALE----EPDDTNSESQ----NSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLF 1234          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000005760.1 (SMESG000005760.1)

HSP 1 Score: 3490.66 bits (9050), Expect = 0.000e+0
Identity = 1879/1885 (99.68%), Postives = 1882/1885 (99.84%), Query Frame = 1
Query:  454 MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVXXXXXXXXXXXXXXXQSILSINKSFETTSSRNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFXXXXXXXXXXXXXQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINKS 6108
            MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKLYNQ+QIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDS SKHR+PFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFSILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSSRNINTTILQEDASIMP MKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDD+NSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLKLVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKIN S
Sbjct:    1 MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKLYNQMQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVNTISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDISSKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKDKVYPQSVASRSSSINSCAYQIPRLTDSSSKHRKPFNKNQNKTKRKNYLSICQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFSILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHNYRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSSRNINTTILQEDASIMPRMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKLVSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSISETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKMNYLLAREKALEEPDDTNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLKLVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKNNNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNLSRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNNKPMKISENPKSLKSKINVS 1885          

HSP 2 Score: 62.003 bits (149), Expect = 7.098e-9
Identity = 51/196 (26.02%), Postives = 85/196 (43.37%), Query Frame = 1
Query:   79 RKKYSLDSFPSSVF-YEANEFLEIENEIECLDILPQDNRSRRETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            R   SLDS   S + ++ N  L  E  +E     P D  S  +    N S  N  +    +  +     +   P    I+   Y    ++F  +   R  C  I+    F+ L +  I +NCITL+M +P    +  + +   +   +YI    FT EM+IK+++ G L GD  Y    WN +D F+V +  +++ 
Sbjct: 1049 RYSLSLDSEGKSNYQFKMNYLLAREKALE----EPDDTNSESQ----NSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPH--SKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLF 1234          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000000986.1 (SMESG000000986.1)

HSP 1 Score: 878.241 bits (2268), Expect = 0.000e+0
Identity = 514/766 (67.10%), Postives = 606/766 (79.11%), Query Frame = 1
Query: 3712 QNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREVQRDLDEEAKELARE---KQLKDEENNENTLIDIEI--DKNNNFLDKKENNSINQNQIKKLSC----------GDCHSINAQLRNVSCQNVHLVNIYS-----KNLSRSHPHLFVNLDSASF 5949
            ++SS +N  +D   E+ E ++C    C      F   YY SL+V S DN+FR  CQ++++KK FD +VLFFI LNCITLAMERPSIP  S+ER FLT+TNY+FT+IF  EM IKV++KGLL+G  +YL+SGWN+MDG LV ISL D+ I +++GHS++IFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGI GVQLFKGKFYYC GP +  VK  ++C    +N WKNQ+YNFDNLGQALM+LFVLSSKDGWVQIMYTG+DAVGVN QPQEN+NEW L+YFISFLLLV FFVLNMFVGVVVENFHKC+ENQEKEEKARRAAKRARRLEKK+K +  P YW+ Y  WR  I+ +V SKYFDLAIAG+IGLNVITMA EF+MMP  L+ ALK  NY FTS F++EAL+K +A G  RYI D+WNQLD++IVILS++G+V++E+++ ++PIN TI+RV RVLRIARVLKLLKMA GIRALLDTVIQALPQVGNLGLLFFLLF+IFAALGVELFGRLECSD NPC GL RHAHFENFGMAFLTLFRIATGDNWNGIMKDTLR KC +SE+CQ+NCCVS IIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDD E+E+EVQR++DEE KE  RE     +++ EN  N  I  +I    NNN + + +   I  N++   +               S      N S   +H++N+ +     + LS+S P L VNL+  S 
Sbjct: 1192 EDSSLLNQDQDDHNENEEKILCNYFWCSWLSKFFRAHYYDSLFVLSPDNRFRNSCQNLMEKKVFDNIVLFFIALNCITLAMERPSIPQKSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIMIVLIIGHSSKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGIFGVQLFKGKFYYCSGPDVAHVKNFSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGLDAVGVNQQPQENYNEWYLLYFISFLLLVGFFVLNMFVGVVVENFHKCRENQEKEEKARRAAKRARRLEKKKKKMMQPPYWKFYPHWRLLIHGLVNSKYFDLAIAGIIGLNVITMAMEFYMMPVELEVALKSLNYIFTSVFIIEALIKTIAYGPKRYIIDRWNQLDVLIVILSVSGIVMEEMKNKYIPINMTIIRVFRVLRIARVLKLLKMAKGIRALLDTVIQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDYNPCTGLSRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLRNKCDNSEECQKNCCVSPIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEESHKMMCDDDEIEKEVQREIDEENKEKLRENITSDVEEIENRGNKEIKEQIIEPNNNNIVYEPKKIEIKCNRLSNSAAVKFNRNANSQDSADSGKTPPDNGSGGTLHILNLPASRKLHEQLSQSQPLLLVNLNPDSL 1957          

HSP 2 Score: 457.988 bits (1177), Expect = 1.671e-130
Identity = 252/374 (67.38%), Postives = 302/374 (80.75%), Query Frame = 1
Query:  454 MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRH 1575
            MY+PC D +C+T +C+I+  FD+ IF FF  E++IKI AMG LG  GYF ++WNRLDM IVI G IE +PSTG  + LTAIRT+RVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGI+GVQMWQG+LRNRCYL+LN S+RHPGLNLS +YQ+N+T    +  KDFICS D  NG++KCSDIPPS++ L +   ++C   A P +DN+PT  SCVNWNQYY+ C+    NPY GAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDW YFV+LIIIGSFFMINLCLVVIA+QFSETK+RETERM +ER++  S++    S  EPQ+CY EIIKLI++    + + ++K Y +   +H
Sbjct:    1 MYKPCEDTSCKTIRCKILGGFDHCIFMFFAVELMIKICAMGLLGPSGYFSESWNRLDMVIVIAGLIECIPSTGE-MSLTAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQMWQGILRNRCYLHLNSSYRHPGLNLSYYYQVNQTTTQLSPHKDFICSPDNFNGLVKCSDIPPSVIWLPSGEARVCNDSALPNSDNMPTNVSCVNWNQYYSKCEARANNPYYGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWSYFVSLIIIGSFFMINLCLVVIATQFSETKKRETERMLMERKKQQSTSTLG-SSIEPQSCYHEIIKLISRFCRHLLKTVIKRYERFSEKH 372          

HSP 3 Score: 400.979 bits (1029), Expect = 1.506e-112
Identity = 218/331 (65.86%), Postives = 265/331 (80.06%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLL-------HNYRQ--SLKKRRANNKQLRIMQHQSENLVLLTNS-----IQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNE 3105
            ++K+K++V    FQRGILIAILINTLSMGIEYH QPV LT TLE+SN++F+ LFL+EM+LK++ EG+LGYI NGFN+FDG IV+LG++EL Q+GG SGLS+LRTFRLLRILKLVRFMPALR QL VM++T+DNVATFFALLILFIFIFSILGMSLFGCKFC  +N KR CDRKNFDSLLW+IVTVFQILTQEDWN VL +GM++TSSWAALYF+ALMTFGNYVLFNLLVAILVEGFS+ER+DE KL        HN  +  +L K  A+  Q    +   E   +++N+     I+K    + Q +    P+I HT ATPMP+PR+SP+N+
Sbjct:  622 RKKMKSIVAHRYFQRGILIAILINTLSMGIEYHNQPVLLTNTLEYSNMIFNGLFLVEMLLKILAEGLLGYISNGFNVFDGGIVILGLVELAQEGGASGLSVLRTFRLLRILKLVRFMPALRLQLIVMLKTMDNVATFFALLILFIFIFSILGMSLFGCKFCKFKNGKRKCDRKNFDSLLWAIVTVFQILTQEDWNAVLISGMSQTSSWAALYFVALMTFGNYVLFNLLVAILVEGFSTERVDEAKLAEKIHLEKHNEEKMANLMKHEAHKNQETTKKSSIEPQEVISNNKNIAIIRKGSLNQKQVM----PIITHTDATPMPSPRASPFND 948          

HSP 4 Score: 112.079 bits (279), Expect = 4.210e-24
Identity = 75/281 (26.69%), Postives = 142/281 (50.53%), Query Frame = 1
Query: 3859 QFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLF---LTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            +FR+K + IV  + F   +L  I +N +++ +E      H++  L    L  +N IF  +F  EM++K+L++GLL     Y+ +G+N+ DG +V + L++L      G ++ +  +LR FRLLR L+ +R +   P L+L +  +L ++  +     +   F  IF ILG+ LF  KF                  K+   + K  + NFD+L  A++ +F + +++ W  ++ +G+             + WA +YF++ +    + + N+ V ++VE F
Sbjct:  620 RFRKKMKSIVAHRYFQRGILIAILINTLSMGIEY-----HNQPVLLTNTLEYSNMIFNGLFLVEMLLKILAEGLL----GYISNGFNVFDGGIVILGLVELAQE---GGASGL-SVLRTFRLLRILKLVRFM---PALRLQLIVMLKTMDNVATFFALLILFIFIFSILGMSLFGCKF-----------------CKFKNGKRKCDRKNFDSLLWAIVTVFQILTQEDWNAVLISGMSQT----------SSWAALYFVALMTFGNYVLFNLLVAILVEGF 857          

HSP 5 Score: 84.3445 bits (207), Expect = 1.045e-15
Identity = 64/208 (30.77%), Postives = 111/208 (53.37%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTS-----GLSILRTFRLLRI---LKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDR-KNFDSLLWSIVTVFQILTQEDWNTVLYNGM 2760
            I  LVN   F   I   I +N ++M +E++  PV+L   L+  N +F+ +F+IE ++K I  G   YI + +N  D +IV+L V  +  +   +      ++I+R FR+LRI   LKL++    +R  L  +I+ +  V     L  L  FIF+ LG+ LFG   C+  N      R  +F++   + +T+F+I T ++WN ++ + +
Sbjct: 1564 IHGLVNSKYFDLAIAGIIGLNVITMAMEFYMMPVELEVALKSLNYIFTSVFIIEALIKTIAYGPKRYIIDRWNQLDVLIVILSVSGIVMEEMKNKYIPINMTIIRVFRVLRIARVLKLLKMAKGIRALLDTVIQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDYNPCTGLSRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 1771          

HSP 6 Score: 76.2554 bits (186), Expect = 3.204e-13
Identity = 71/269 (26.39%), Postives = 133/269 (49.44%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLEF---SNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGV----IELCQQGGTSGLSILRTFRLLRILK---LVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CT------------IENNKRTC---DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            F   +L  I +N +++ +E    P Q ++  EF   +N VF+++F +EM +K+I +G+L     Y+++G+N+ DG++VV+ +    I L     +    ILR FRLLR L+   ++   P L+  +  ++ ++  +     +   F  IF I G+ LF  KF  C+              N+KR      + NFD+L  +++++F + +++ W  ++Y G+               W  LYFI+ +    + + N+ V ++VE F   R
Sbjct: 1255 FDNIVLFFIALNCITLAMERPSIP-QKSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIMIVLIIGHSSKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGIFGVQLFKGKFYYCSGPDVAHVKNFSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGLDAVGVNQQPQENYNEWYLLYFISFLLLVGFFVLNMFVGVVVENFHKCR 1522          

HSP 7 Score: 73.9442 bits (180), Expect = 1.407e-12
Identity = 64/209 (30.62%), Postives = 109/209 (52.15%), Query Frame = 1
Query: 4813 KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIM 5439
            ++R+ + S+V  +YF   I   I +N ++M  E+H  P +L   L+  N  F   F++E LLK++A GL  YIS+ +N  D  IVIL +  +  +   S         + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C   N      R  +F++   A +T+F+I T ++WN ++
Sbjct:  620 RFRKKMKSIVAHRYFQRGILIAILINTLSMGIEYHNQPVLLTNTLEYSNMIFNGLFLVEMLLKILAEGLLGYISNGFNVFDGGIVILGLVELAQEGGASG--------LSVLRTFRLLRILKLVRFMPALRLQLIVMLKTMDNVATFFALLILFIFIFSILGMSLFGCKFCKFKNGKRKCDRK-NFDSLLWAIVTVFQILTQEDWNAVL 819          

HSP 8 Score: 63.1586 bits (152), Expect = 2.786e-9
Identity = 43/118 (36.44%), Postives = 69/118 (58.47%), Query Frame = 1
Query: 4024 IIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            + F  E++IK+ + GLL G   Y    WN +D  +V   L++   +   G  +     L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQ+++G
Sbjct:   27 MFFAVELMIKICAMGLL-GPSGYFSESWNRLDMVIVIAGLIECIPST--GEMS-----LTAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQMWQG 136          

HSP 9 Score: 58.9214 bits (141), Expect = 5.646e-8
Identity = 33/114 (28.95%), Postives = 61/114 (53.51%), Query Frame = 1
Query:  322 FYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            +Y ++F +   N+  R+ C  ++    F+ + +  IA+NCITL+M +P   Q  ++ + + +   +Y+    F  EM IK+IA G L G   Y    WN +D  +V++   +I+
Sbjct: 1229 YYDSLFVLSPDNRF-RNSCQNLMEKKVFDNIVLFFIALNCITLAMERPSIPQ--KSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIM 1339          

HSP 10 Score: 54.6842 bits (130), Expect = 1.224e-6
Identity = 30/80 (37.50%), Postives = 47/80 (58.75%), Query Frame = 1
Query:  517 DYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRI 756
            + I    F  EM++KI+A G LG   Y  + +N  D  IVI+G +E L   G   GL+ +RT R+LR L+ +  +P++R+
Sbjct:  668 NMIFNGLFLVEMLLKILAEGLLG---YISNGFNVFDGGIVILGLVE-LAQEGGASGLSVLRTFRLLRILKLVRFMPALRL 743          

HSP 11 Score: 54.299 bits (129), Expect = 1.342e-6
Identity = 27/90 (30.00%), Postives = 53/90 (58.89%), Query Frame = 1
Query: 2287 FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            F + +F + F +E+++K+   G+LG   Y    +N  D +IV+ G+IE     G   L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:   21 FDHCIF-MFFAVELMIKICAMGLLGPSGYFSESWNRLDMVIVIAGLIECIPSTGEMSLTAIRTVRVLRPLRAINRIPSMRILVMLLLDTL 109          

HSP 12 Score: 52.7582 bits (125), Expect = 4.244e-6
Identity = 24/81 (29.63%), Postives = 46/81 (56.79%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE---TERMWIER 1428
            NFD++ +A V +FQ+++ E W  ++       S W  +YFVAL+  G++ + NL + ++   FS  +  E    E++ +E+
Sbjct:  795 NFDSLLWAIVTVFQILTQEDWNAVLISGMSQTSSWAALYFVALMTFGNYVLFNLLVAILVEGFSTERVDEAKLAEKIHLEK 875          

HSP 13 Score: 52.7582 bits (125), Expect = 4.464e-6
Identity = 26/80 (32.50%), Postives = 46/80 (57.50%), Query Frame = 1
Query: 4474 NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            NFDN+G A +A+F + S + WV IMY   DA          H+ W   YF+S +++ +FF++N+ + V+   F + ++ +
Sbjct:  247 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWSYFVSLIIIGSFFMINLCLVVIATQFSETKKRE 316          

HSP 14 Score: 52.373 bits (124), Expect = 5.370e-6
Identity = 25/97 (25.77%), Postives = 54/97 (55.67%), Query Frame = 1
Query: 1147 YTDCQPGEKNPYEGA-INFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            +++C   ++N ++    NFDN+G A +++F + S + WV IMY   DA          ++ W  +YF++ +++  FF++N+ + V+   F + +  +
Sbjct: 1429 FSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGLDAVGVNQQPQENYNEWYLLYFISFLLLVGFFVLNMFVGVVVENFHKCRENQ 1525          
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Planmine SMEST
Match: SMESG000000986.1 (SMESG000000986.1)

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 336/486 (69.14%), Postives = 402/486 (82.72%), Query Frame = 1
Query: 3712 QNSSRMNNVEDPDEEDMEAMICLSKCCPQPKGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQXXXXXXXXXXXXXXXXXXXXXHLRAPLYWENYSKWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIAR 5169
            ++SS +N  +D   E+ E ++C    C      F   YY SL+V S DN+FR  CQ++++KK FD +VLFFI LNCITLAMERPSIP  S+ER FLT+TNY+FT+IF  EM IKV++KGLL+G  +YL+SGWN+MDG LV ISL D+ I +++GHS++IFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGI GVQLFKGKFYYC GP +  VK  ++C    +N WKNQ+YNFDNLGQALM+LFVLSSKDGWVQIMYTG+DAVGVN QPQEN+NEW L+YFISFLLLV FFVLNMFVGVVVENFHKC+ENQEKEEKARRAAKRARRLEKK+K +  P YW+ Y  WR  I+ +V SKYFDLAIAG+IGLNVITMA EF+MMP  L+ ALK  NY FTS F++EAL+K +A G  RYI D+WNQLD++IVILS++G+V++E+++ ++PIN TI+RV RVLRIAR
Sbjct: 1192 EDSSLLNQDQDDHNENEEKILCNYFWCSWLSKFFRAHYYDSLFVLSPDNRFRNSCQNLMEKKVFDNIVLFFIALNCITLAMERPSIPQKSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIMIVLIIGHSSKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGIFGVQLFKGKFYYCSGPDVAHVKNFSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGLDAVGVNQQPQENYNEWYLLYFISFLLLVGFFVLNMFVGVVVENFHKCRENQEKEEKARRAAKRARRLEKKKKKMMQPPYWKFYPHWRLLIHGLVNSKYFDLAIAGIIGLNVITMAMEFYMMPVELEVALKSLNYIFTSVFIIEALIKTIAYGPKRYIIDRWNQLDVLIVILSVSGIVMEEMKNKYIPINMTIIRVFRVLRIAR 1677          

HSP 2 Score: 456.062 bits (1172), Expect = 1.016e-131
Identity = 252/374 (67.38%), Postives = 302/374 (80.75%), Query Frame = 1
Query:  454 MYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINRTLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFTDNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRETERMWIERRRFNSSNCSSISKDEPQTCYLEXXXXXXXXXXXXYRRIVKLYNQIQIRH 1575
            MY+PC D +C+T +C+I+  FD+ IF FF  E++IKI AMG LG  GYF ++WNRLDM IVI G IE +PSTG  + LTAIRT+RVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGI+GVQMWQG+LRNRCYL+LN S+RHPGLNLS +YQ+N+T    +  KDFICS D  NG++KCSDIPPS++ L +   ++C   A P +DN+PT  SCVNWNQYY+ C+    NPY GAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDW YFV+LIIIGSFFMINLCLVVIA+QFSETK+RETERM +ER++  S++    S  EPQ+CY EIIKLI++    + + ++K Y +   +H
Sbjct:    1 MYKPCEDTSCKTIRCKILGGFDHCIFMFFAVELMIKICAMGLLGPSGYFSESWNRLDMVIVIAGLIECIPSTGE-MSLTAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQMWQGILRNRCYLHLNSSYRHPGLNLSYYYQVNQTTTQLSPHKDFICSPDNFNGLVKCSDIPPSVIWLPSGEARVCNDSALPNSDNMPTNVSCVNWNQYYSKCEARANNPYYGAINFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWSYFVSLIIIGSFFMINLCLVVIATQFSETKKRETERMLMERKKQQSTSTLG-SSIEPQSCYHEIIKLISRFCRHLLKTVIKRYERFSEKH 372          

HSP 3 Score: 399.438 bits (1025), Expect = 4.284e-113
Identity = 218/331 (65.86%), Postives = 265/331 (80.06%), Query Frame = 1
Query: 2155 QQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLL-------HNYRQ--SLKKRRANNKQLRIMQHQSENLVLLTNS-----IQKDQTGENQKISSQPPVIIHTAATPMPTPRSSPYNE 3105
            ++K+K++V    FQRGILIAILINTLSMGIEYH QPV LT TLE+SN++F+ LFL+EM+LK++ EG+LGYI NGFN+FDG IV+LG++EL Q+GG SGLS+LRTFRLLRILKLVRFMPALR QL VM++T+DNVATFFALLILFIFIFSILGMSLFGCKFC  +N KR CDRKNFDSLLW+IVTVFQILTQEDWN VL +GM++TSSWAALYF+ALMTFGNYVLFNLLVAILVEGFS+ER+DE KL        HN  +  +L K  A+  Q    +   E   +++N+     I+K    + Q +    P+I HT ATPMP+PR+SP+N+
Sbjct:  622 RKKMKSIVAHRYFQRGILIAILINTLSMGIEYHNQPVLLTNTLEYSNMIFNGLFLVEMLLKILAEGLLGYISNGFNVFDGGIVILGLVELAQEGGASGLSVLRTFRLLRILKLVRFMPALRLQLIVMLKTMDNVATFFALLILFIFIFSILGMSLFGCKFCKFKNGKRKCDRKNFDSLLWAIVTVFQILTQEDWNAVLISGMSQTSSWAALYFVALMTFGNYVLFNLLVAILVEGFSTERVDEAKLAEKIHLEKHNEEKMANLMKHEAHKNQETTKKSSIEPQEVISNNKNIAIIRKGSLNQKQVM----PIITHTDATPMPSPRASPFND 948          

HSP 4 Score: 109.383 bits (272), Expect = 2.538e-23
Identity = 75/281 (26.69%), Postives = 142/281 (50.53%), Query Frame = 1
Query: 3859 QFRQKCQHIVDKKSFDYLVLFFITLNCITLAMERPSIPPHSKERLF---LTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGPHLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENF 4692
            +FR+K + IV  + F   +L  I +N +++ +E      H++  L    L  +N IF  +F  EM++K+L++GLL     Y+ +G+N+ DG +V + L++L      G ++ +  +LR FRLLR L+ +R +   P L+L +  +L ++  +     +   F  IF ILG+ LF  KF                  K+   + K  + NFD+L  A++ +F + +++ W  ++ +G+             + WA +YF++ +    + + N+ V ++VE F
Sbjct:  620 RFRKKMKSIVAHRYFQRGILIAILINTLSMGIEY-----HNQPVLLTNTLEYSNMIFNGLFLVEMLLKILAEGLL----GYISNGFNVFDGGIVILGLVELAQE---GGASGL-SVLRTFRLLRILKLVRFM---PALRLQLIVMLKTMDNVATFFALLILFIFIFSILGMSLFGCKF-----------------CKFKNGKRKCDRKNFDSLLWAIVTVFQILTQEDWNAVLISGMSQT----------SSWAALYFVALMTFGNYVLFNLLVAILVEGF 857          

HSP 5 Score: 75.0998 bits (183), Expect = 7.137e-13
Identity = 88/333 (26.43%), Postives = 164/333 (49.25%), Query Frame = 1
Query: 4813 KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVXXXXXXXXXXXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTLREKCISSEDCQRNCCVSAIIAPIYFVIFXXXXXXXXXXXXXXXXXKHLEESHKMMCDDYELEREV--QRDLDEEAKELAREKQLKDEENNENTLIDI-EIDKNNNFLDKKENNSINQNQI 5802
            ++R+ + S+V  +YF   I   I +N ++M  E+H  P +L   L+  N  F   F++E LLK++A GL  YIS+ +N  D  IVIL +  +  +   S         + V+R  R+ R+LKL++    +R  L  +++ +  V     L  L  +IF+ LG+ LFG   C   N      R  +F++   A +T+F+I T ++WN ++   + +              ++  A +YFV  +    +VL N++VA+L++          D+ +L  ++  ++  +E+   L + +  K++E  + + I+  E+  NN  +      S+NQ Q+
Sbjct:  620 RFRKKMKSIVAHRYFQRGILIAILINTLSMGIEYHNQPVLLTNTLEYSNMIFNGLFLVEMLLKILAEGLLGYISNGFNVFDGGIVILGLVELAQEGGASG--------LSVLRTFRLLRILKLVRFMPALRLQLIVMLKTMDNVATFFALLILFIFIFSILGMSLFGCKFCKFKNGKRKCDR-KNFDSLLWAIVTVFQILTQEDWNAVLISGMSQ--------------TSSWAALYFVALMTFGNYVLFNLLVAILVEGFSTER---VDEAKLAEKIHLEKHNEEKMANLMKHEAHKNQETTKKSSIEPQEVISNNKNIAIIRKGSLNQKQV 926          

HSP 6 Score: 75.0998 bits (183), Expect = 7.444e-13
Identity = 71/269 (26.39%), Postives = 133/269 (49.44%), Query Frame = 1
Query: 2191 FQRGILIAILINTLSMGIEYHQQPVQLTQTLEF---SNIVFSILFLIEMILKLIGEGIL----GYIKNGFNLFDGIIVVLGV----IELCQQGGTSGLSILRTFRLLRILK---LVRFMPALRQQLFVMIRTIDNVATXXXXXXXXXXXXXXXGMSLFGCKF--CT------------IENNKRTC---DRKNFDSLLWSIVTVFQILTQEDWNTVLYNGMTRTS----------SWAALYFIALMTFGNYVLFNLLVAILVEGFSSER 2874
            F   +L  I +N +++ +E    P Q ++  EF   +N VF+++F +EM +K+I +G+L     Y+++G+N+ DG++VV+ +    I L     +    ILR FRLLR L+   ++   P L+  +  ++ ++  +     +   F  IF I G+ LF  KF  C+              N+KR      + NFD+L  +++++F + +++ W  ++Y G+               W  LYFI+ +    + + N+ V ++VE F   R
Sbjct: 1255 FDNIVLFFIALNCITLAMERPSIP-QKSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIMIVLIIGHSSKIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGIFGVQLFKGKFYYCSGPDVAHVKNFSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGLDAVGVNQQPQENYNEWYLLYFISFLLLVGFFVLNMFVGVVVENFHKCR 1522          

HSP 7 Score: 64.3142 bits (155), Expect = 1.228e-9
Identity = 41/122 (33.61%), Postives = 67/122 (54.92%), Query Frame = 1
Query: 2164 IKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIVFSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTS-----GLSILRTFRLLRILK--LVRFMP 2508
            I  LVN   F   I   I +N ++M +E++  PV+L   L+  N +F+ +F+IE ++K I  G   YI + +N  D +IV+L V  +  +   +      ++I+R FR+LRI +    RFMP
Sbjct: 1564 IHGLVNSKYFDLAIAGIIGLNVITMAMEFYMMPVELEVALKSLNYIFTSVFIIEALIKTIAYGPKRYIIDRWNQLDVLIVILSVSGIVMEEMKNKYIPINMTIIRVFRVLRIARGEKFRFMP 1685          

HSP 8 Score: 62.003 bits (149), Expect = 6.556e-9
Identity = 43/118 (36.44%), Postives = 69/118 (58.47%), Query Frame = 1
Query: 4024 IIFTGEMIIKVLSKGLLIGDQAYLKSGWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKG 4377
            + F  E++IK+ + GLL G   Y    WN +D  +V   L++   +   G  +     L   R +R LRPLR I+R P ++++V  LL +L  +GN++L+C   F IFGI+GVQ+++G
Sbjct:   27 MFFAVELMIKICAMGLL-GPSGYFSESWNRLDMVIVIAGLIECIPST--GEMS-----LTAIRTVRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIVGVQMWQG 136          

HSP 9 Score: 61.2326 bits (147), Expect = 9.902e-9
Identity = 68/235 (28.94%), Postives = 122/235 (51.91%), Query Frame = 1
Query: 4813 KWRRTINSVVTSKYFDLAIAGVIGLNVITMATEFHMMPQVLKTA--LKVFNYFFTSTFVXXXXXXXXX----XXXXRYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMRVLRIARVLKLLKMASGIRALLDTVIQALPQVGNXXXXXXXXXXXXXXXXVELF-GRL---------------ECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL 5451
            ++R +  +++  K FD  +   I LN IT+A E   +PQ  +    L + NY FT  F +E  +K++A    +G   Y+   WN +D ++V++S+A ++I  +  +   I   I+RV R+LR  R L+++  A G++ ++ T++ +L  +GN+ L+    F IF   GV+LF G+                EC++        +  +F+N G A ++LF +++ D W  IM   L
Sbjct: 1241 RFRNSCQNLMEKKVFDNIVLFFIALNCITLAMERPSIPQKSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIMIVLIIGHSSKIF-GILRVFRLLRTLRPLRVISRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGIFGVQLFKGKFYYCSGPDVAHVKNFSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGL 1474          

HSP 10 Score: 56.9954 bits (136), Expect = 1.837e-7
Identity = 33/114 (28.95%), Postives = 61/114 (53.51%), Query Frame = 1
Query:  322 FYATVFHIFHQNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQIMQAFDYIIFAFFTCEMVIKIIAMGFL-GDLGYFGDTWNRLDMFIVIVGSIEIL 660
            +Y ++F +   N+  R+ C  ++    F+ + +  IA+NCITL+M +P   Q  ++ + + +   +Y+    F  EM IK+IA G L G   Y    WN +D  +V++   +I+
Sbjct: 1229 YYDSLFVLSPDNRF-RNSCQNLMEKKVFDNIVLFFIALNCITLAMERPSIPQ--KSREREFLTITNYVFTVIFAVEMTIKVIAKGLLVGKHSYLRSGWNVMDGLLVVISLADIM 1339          

HSP 11 Score: 53.9138 bits (128), Expect = 1.666e-6
Identity = 30/80 (37.50%), Postives = 47/80 (58.75%), Query Frame = 1
Query:  517 DYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSIEILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRI 756
            + I    F  EM++KI+A G LG   Y  + +N  D  IVI+G +E L   G   GL+ +RT R+LR L+ +  +P++R+
Sbjct:  668 NMIFNGLFLVEMLLKILAEGLLG---YISNGFNVFDGGIVILGLVE-LAQEGGASGLSVLRTFRLLRILKLVRFMPALRL 743          

HSP 12 Score: 53.1434 bits (126), Expect = 3.293e-6
Identity = 27/90 (30.00%), Postives = 53/90 (58.89%), Query Frame = 1
Query: 2287 FSNIVFSILFLIEMILKLIGEGILG---YIKNGFNLFDGIIVVLGVIELCQQGGTSGLSILRTFRLLRILKLVRFMPALRQQLFVMIRTI 2547
            F + +F + F +E+++K+   G+LG   Y    +N  D +IV+ G+IE     G   L+ +RT R+LR L+ +  +P++R  + +++ T+
Sbjct:   21 FDHCIF-MFFAVELMIKICAMGLLGPSGYFSESWNRLDMVIVIAGLIECIPSTGEMSLTAIRTVRVLRPLRAINRIPSMRILVMLLLDTL 109          

HSP 13 Score: 51.9878 bits (123), Expect = 6.677e-6
Identity = 26/80 (32.50%), Postives = 46/80 (57.50%), Query Frame = 1
Query: 4474 NFDNLGQALMALFVLSSKDGWVQIMYTGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKCQENQ 4713
            NFDN+G A +A+F + S + WV IMY   DA          H+ W   YF+S +++ +FF++N+ + V+   F + ++ +
Sbjct:  247 NFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWSYFVSLIIIGSFFMINLCLVVIATQFSETKKRE 316          

HSP 14 Score: 51.6026 bits (122), Expect = 8.522e-6
Identity = 27/98 (27.55%), Postives = 52/98 (53.06%), Query Frame = 1
Query: 1195 NFDNIGFAWVAIFQVISMESWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE---TERMWIERRRFNSSNCSSISKDEPQ 1479
            NFD++ +A V +FQ+++ E W  ++       S W  +YFVAL+  G++ + NL + ++   FS  +  E    E++ +E+   N    +++ K E  
Sbjct:  795 NFDSLLWAIVTVFQILTQEDWNAVLISGMSQTSSWAALYFVALMTFGNYVLFNLLVAILVEGFSTERVDEAKLAEKIHLEKH--NEEKMANLMKHEAH 890          

HSP 15 Score: 51.6026 bits (122), Expect = 9.428e-6
Identity = 25/97 (25.77%), Postives = 54/97 (55.67%), Query Frame = 1
Query: 1147 YTDCQPGEKNPYEGA-INFDNIGFAWVAIFQVISMESWVDIMYYVQDA----------HSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRRE 1404
            +++C   ++N ++    NFDN+G A +++F + S + WV IMY   DA          ++ W  +YF++ +++  FF++N+ + V+   F + +  +
Sbjct: 1429 FSECNNDKRNTWKNQQYNFDNLGQALMSLFVLSSKDGWVQIMYTGLDAVGVNQQPQENYNEWYLLYFISFLLLVGFFVLNMFVGVVVENFHKCRENQ 1525          
The following BLAST results are available for this feature:
BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CACNA1H6.484e-1564.24calcium voltage-gated channel subunit alpha1 H [So... [more]
CACNA1H2.988e-10360.80calcium voltage-gated channel subunit alpha1 H [So... [more]
CACNA1H2.988e-10360.80calcium voltage-gated channel subunit alpha1 H [So... [more]
CACNA1H2.722e-1663.28calcium voltage-gated channel subunit alpha1 H [So... [more]
CACNA1H1.926e-1663.84calcium voltage-gated channel subunit alpha1 H [So... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cca-13.504e-10560.66Voltage-dependent T-type calcium channel subunit a... [more]
cca-13.504e-10560.66Voltage-dependent T-type calcium channel subunit a... [more]
cca-13.504e-10560.66Voltage-dependent T-type calcium channel subunit a... [more]
cca-13.372e-10560.66Voltage-dependent T-type calcium channel subunit a... [more]
cca-13.372e-10560.66Voltage-dependent T-type calcium channel subunit a... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ca-alpha1T3.580e-11770.13gene:FBgn0264386 transcript:FBtr0304609[more]
Ca-alpha1T4.045e-11770.13gene:FBgn0264386 transcript:FBtr0112953[more]
Ca-alpha1T3.265e-11770.13gene:FBgn0264386 transcript:FBtr0089393[more]
Ca-alpha1T4.757e-11770.13gene:FBgn0264386 transcript:FBtr0304610[more]
Ca-alpha1T7.466e-11570.13gene:FBgn0264386 transcript:FBtr0334984[more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cacna1ha3.550e-947.29calcium channel, voltage-dependent, T type, alpha ... [more]
cacna1hb1.734e-8362.14calcium channel, voltage-dependent, T type, alpha ... [more]
cacna1ia3.072e-9163.24calcium voltage-gated channel subunit alpha1 Ia [S... [more]
cacna1ia4.047e-9163.24calcium voltage-gated channel subunit alpha1 Ia [S... [more]
cacna1ia1.168e-9563.24calcium voltage-gated channel subunit alpha1 Ia [S... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
efhd14.588e-11460.15EF-hand domain family member D1 [Source:Xenbase;Ac... [more]
efhd11.783e-11363.67EF-hand domain family member D1 [Source:Xenbase;Ac... [more]
efhd13.473e-11459.91EF-hand domain family member D1 [Source:Xenbase;Ac... [more]
efhd13.009e-11458.41EF-hand domain family member D1 [Source:Xenbase;Ac... [more]
efhd14.025e-11161.71EF-hand domain family member D1 [Source:Xenbase;Ac... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cacna1h7.836e-10561.76calcium channel, voltage-dependent, T type, alpha ... [more]
Cacna1h7.836e-10561.76calcium channel, voltage-dependent, T type, alpha ... [more]
Cacna1h1.189e-9261.76calcium channel, voltage-dependent, T type, alpha ... [more]
Cacna1g1.579e-10263.40calcium channel, voltage-dependent, T type, alpha ... [more]
Cacna1g1.838e-10263.40calcium channel, voltage-dependent, T type, alpha ... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O95180|CAC1H_HUMAN1.337e-10260.47Voltage-dependent T-type calcium channel subunit a... [more]
sp|Q9EQ60|CAC1H_RAT6.735e-10463.68Voltage-dependent T-type calcium channel subunit a... [more]
sp|O88427|CAC1H_MOUSE3.674e-10361.41Voltage-dependent T-type calcium channel subunit a... [more]
sp|Q9Z0Y8|CAC1I_RAT5.630e-9963.67Voltage-dependent T-type calcium channel subunit a... [more]
sp|Q9P0X4|CAC1I_HUMAN5.233e-9863.67Voltage-dependent T-type calcium channel subunit a... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G0YP352.166e-1183.58Voltage operated calcium channel Cav3 (Fragment) O... [more]
X1WBF77.943e-647.29Voltage-dependent T-type calcium channel subunit a... [more]
A0A2C9KE735.441e-10554.69Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9KE955.136e-10555.21Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A2C9KE615.546e-10553.75Uncharacterized protein OS=Biomphalaria glabrata O... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cacna1ha1.397e-9758.26calcium channel, voltage-dependent, T type, alpha ... [more]
cacna1ha1.760e-9757.16calcium channel, voltage-dependent, T type, alpha ... [more]
cacna1ib8.855e-18059.87calcium voltage-gated channel subunit alpha1 Ib [S... [more]
ENSAMXT00000033959.12.616e-8751.90pep primary_assembly:Astyanax_mexicanus-2.0:4:2732... [more]
ENSAMXT00000056794.11.865e-8251.80pep primary_assembly:Astyanax_mexicanus-2.0:2:2726... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000001901.11.734e-9361.05pep scaffold:Pmarinus_7.0:GL477793:3961:23506:1 ge... [more]
ENSPMAT00000001900.12.109e-9356.11pep scaffold:Pmarinus_7.0:GL477793:3961:23576:1 ge... [more]
ENSPMAT00000001904.11.732e-6569.43pep scaffold:Pmarinus_7.0:GL477793:127370:134209:1... [more]
ENSPMAT00000009098.11.212e-6064.48pep scaffold:Pmarinus_7.0:GL478807:14086:32911:1 g... [more]
ENSPMAT00000008004.18.154e-5429.17pep scaffold:Pmarinus_7.0:GL477228:88466:118438:1 ... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
CCH11.443e-1423.26Voltage-gated high-affinity calcium channel; invol... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO368442.212e-17851.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO354972.987e-15753.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO354987.802e-8153.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO469918.327e-6133.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO356618.918e-6131.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cacna1ha4.562e-9263.70voltage-dependent T-type calcium channel subunit a... [more]
cacna1ha4.629e-9263.70voltage-dependent T-type calcium channel subunit a... [more]
cacna1hb2.096e-10464.20calcium voltage-gated channel subunit alpha1 H [So... [more]
cacna1ha2.220e-17157.55voltage-dependent T-type calcium channel subunit a... [more]
ENSORLT00000045672.13.833e-8550.00pep primary_assembly:ASM223467v1:1:25452484:256691... [more]
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BLAST of Voltage-dependent T-type calcium channel subunit alpha vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005760.18.196e-999.68SMESG000005760.1[more]
SMESG000005760.18.228e-998.95SMESG000005760.1[more]
SMESG000005760.17.098e-999.68SMESG000005760.1[more]
SMESG000000986.11.671e-13067.10SMESG000000986.1[more]
SMESG000000986.11.016e-13169.14SMESG000000986.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018324 ID=SMED30018324|Name=Voltage-dependent T-type calcium channel subunit alpha|organism=Schmidtea mediterranea sexual|type=transcript|length=6305bp
AAAATGGATTGCAACTGAACATTTGTAAATTGAAAACTAATTAAAATCAA
CATGAAGAAATCGGAAAATAAAGTCGAGAGAAAAAAATATTCTCTAGATT
CATTTCCATCATCGGTGTTCTATGAGGCGAATGAGTTTTTGGAAATTGAA
AACGAAATTGAATGTCTTGACATCCTTCCACAAGACAATAGATCTCGACG
AGAAACTCTCATCGGAAACAAATCGGATTTTAATCTCACAGACAAATCGA
AAGCTGATCATTGTCAGCAAGAAACCGATGGAACTCCAATCAATCCTAAT
GAAAGTTTGATTTACCACGGATTTTACGCGACTGTCTTTCATATTTTCCA
TCAGAATAAACATCCAAGAGATTGGTGTTTGAAAATAATCACTTGGCCTT
GGTTTGAGCAACTGAGTATGACGATTATTGCTGTAAATTGCATCACATTG
AGCATGTATCAGCCATGTGGTGATCAAAATTGTGAAACATCCAAATGTCA
AATAATGCAAGCATTTGATTATATTATATTTGCCTTTTTCACTTGCGAAA
TGGTTATCAAGATTATTGCGATGGGATTTCTAGGAGATTTGGGATATTTC
GGTGATACTTGGAATCGGTTAGACATGTTCATAGTAATTGTTGGATCAAT
TGAAATTCTTCCTTCCACCGGTAATAATATTGGCCTCACAGCAATCAGAA
CGATAAGAGTGCTCAGGCCGCTACGCGCCATCAACAGAATACCAAGTATG
CGTATTCTCGTCATGTTACTGCTGGATACTTTGCCAATGCTCGGAAATGT
ACTGCTGCTATGTTTCTTTGTTTTCTTTATTTTTGGAATTATTGGAGTTC
AAATGTGGCAAGGCGTGCTCAGAAATCGTTGCTACCTAAACCTCAACGAA
TCCTTTAGACATCCAGGGCTCAATCTTTCATCGTTTTATCAAATTAACCG
AACTCTATTTTTAAATGCGGTCGACAAGGATTTTATTTGTTCAACAGACA
AAAACAATGGAATTTTAAAATGCTCAGATATCCCACCAAGTATTATGTTG
TTGTCGAACAATTCTCTTCAAATCTGCAAGCAACCCGCTCGCCCTTTTAC
CGACAACTTGCCTACGAAGCACAGTTGCGTCAATTGGAATCAGTATTATA
CAGACTGCCAGCCGGGTGAAAAAAACCCTTATGAAGGAGCAATTAATTTC
GATAATATTGGATTTGCTTGGGTGGCTATTTTTCAGGTAATCAGCATGGA
AAGTTGGGTGGATATAATGTATTACGTACAAGATGCTCATTCGTTTTGGG
ATTGGATATATTTTGTTGCGTTAATAATTATTGGCTCCTTTTTTATGATC
AATTTGTGCTTGGTCGTTATTGCCAGTCAGTTTTCTGAAACAAAACGCCG
AGAAACCGAACGCATGTGGATTGAAAGAAGAAGATTCAATTCAAGCAATT
GCTCCTCGATTTCCAAAGACGAACCCCAAACATGTTATTTGGAAATAATC
AAATTAATCACTCAAATCATTTGCAAAATTTATAGACGAATTGTCAAATT
ATATAATCAAATACAAATTCGACATTCTAAAACTGCTCAAGGAAATTATC
AAAACAAACTCAAAAGACAAAGAAAACATTGTAACGTGTGCTTAAATTCC
CGAAGTCAATTTCCCATTGATGATCACTGCCATCTTTATTCTATTGTCAA
TACAATTAGTGGGGATAAACAACACTATTATGCCATAAATAAATATCCTA
TTAATTTTTCAAATATTCCCCCGAAAGCCAGTCCGGAATTATCTGTGATT
GATGTACTCAATTCTCCTAGACATCCTAATCACCTTATTTCTGATATCTC
ATCAAAATCTGTCTCCACTGAAACAAAACGAAAATCTCAATATAATTCCC
AAATTTTATTAGAAATTCCTTCCACCCCAGCTAATCATTCGACCACGAGA
GACAACTATCGATGGGAATTAACAAATAATCCTTTATTAACTTCCAAAGA
TAAAGTCTACCCCCAATCTGTTGCGAGTAGATCTTCGTCTATCAACAGCT
GCGCATATCAAATTCCTCGATTAACAGATAGTTATAGTAAACATCGTAGA
CCTTTTAATAAAAATCAAAATAAAACGAAAAGAAAAAATTATTTATCGAT
ATGTCAACAGAAAATTAAAACATTGGTGAATGATGTTAAATTTCAGAGAG
GGATTCTGATTGCAATTCTTATCAATACTCTTAGCATGGGTATAGAATAT
CATCAGCAACCGGTTCAGCTGACGCAAACATTGGAGTTCAGCAATATTGT
TTTTAGTATCTTATTTCTTATCGAAATGATTTTGAAACTGATAGGCGAAG
GCATTCTTGGATACATCAAAAATGGTTTCAATTTGTTCGATGGAATTATT
GTCGTTTTGGGTGTGATCGAGCTTTGCCAGCAAGGAGGGACCTCAGGTTT
ATCAATTTTAAGGACGTTCAGGTTACTAAGAATTTTAAAATTGGTAAGAT
TTATGCCTGCTCTTCGACAACAATTATTCGTAATGATTCGAACAATCGAC
AATGTCGCTACATTTTTTGCTCTTTTAATATTATTTATTTTCATCTTCAG
TATTTTAGGAATGAGCCTATTTGGTTGTAAATTTTGTACGATAGAAAACA
ATAAACGGACTTGCGATAGAAAAAATTTTGATTCATTGCTTTGGTCAATT
GTAACTGTTTTTCAAATTCTAACTCAAGAGGATTGGAACACTGTGTTATA
CAACGGAATGACTAGAACTTCATCATGGGCTGCACTTTATTTTATTGCTT
TGATGACTTTCGGAAATTATGTTTTATTCAATCTCCTTGTGGCAATATTA
GTCGAAGGATTTTCTTCAGAAAGACTGGATGAACAAAAGCTTCTGCATAA
CTATCGACAATCACTAAAGAAAAGGAGAGCAAATAATAAACAACTTAGAA
TCATGCAACATCAATCAGAAAATTTAGTACTTCTTACGAATTCAATACAA
AAAGATCAAACGGGCGAAAATCAAAAAATATCTAGTCAACCACCAGTTAT
AATTCATACTGCTGCTACCCCAATGCCTACTCCACGGAGTAGCCCTTATA
ATGAATTGGTAACAACTCCTCATACTACTTTTCCTCATACGAATACCTCA
AATTCTCAGTCGATTCTTTCTATAAACAAAAGTTTTGAAACTACCTCAAG
TAGAAATATAAACACTACCATATTACAAGAAGATGCCTCAATAATGCCCG
GAATGAAAAATTCAAAGCAAAATTTAGATAGTACATTATTCGTTGAGCAT
CACATCCCTGTTCAGAAAACTCCCACATCCAATGGCTTGAACGTTAAGCT
GGTTTCTATTCCGATTCCTTATACAACAAACCGATCTAATTTTATCATTC
CATCCAGTATTTTACTACGTCAAGGTTCCATCAACAGTTTCATGGGAAGT
GTCAACAGTGATCGAAGCAAAATTCTTTTGGATGAAAAGAAAAGTATATC
AGAAACGGATGAAAAATCTCTAAGTCGGCAGTCGTCTTTCAAAAGCTTTA
TGGCATATAAAAACAAGATAAATCAGTTAACCATTCCGTTTTCTAATAGA
TATAGTCTTTCATTAGATAGTGAAGGGAAATCAAATTACCAATTTAAAAT
GAATTATCTTTTGGCACGTGAAAAAGCACTTGAAGAACCTGATGATTCAA
ATTCAGAATCACAAAATTCTTCTAGAATGAATAATGTTGAGGATCCAGAT
GAAGAAGATATGGAAGCAATGATTTGTCTCTCAAAATGTTGTCCTCAACC
GAAAGGAATATTTGCCAAACGTTATTATCATTCTCTCTATGTATTTTCAG
TTGACAATCAATTCCGACAGAAATGTCAACACATTGTGGACAAAAAATCT
TTTGATTATCTGGTGCTATTTTTTATTACTTTAAACTGTATTACTCTTGC
CATGGAAAGACCCTCAATTCCTCCACATAGTAAGGAGCGACTGTTTCTTA
CCGTGACAAATTATATATTCACCATAATATTCACTGGAGAAATGATCATA
AAAGTATTATCAAAAGGATTGCTTATTGGTGATCAGGCATATCTGAAAAG
TGGCTGGAATATAATGGATGGTTTTCTTGTTTTTATATCTCTGCTCGACT
TGTTTATAACAATATTATTGGGACACAGCAACAGGATATTTGGGATTTTG
CGAGTGTTTCGTCTACTCAGAACTTTACGGCCATTGCGAGTAATCAGCCG
GGCGCCCGGTTTGAAACTTGTTGTCCAAACGCTGTTATCTTCATTGCGTC
CCATTGGCAATATAGTACTGATATGTTGTACATTTTTCATCATTTTTGGA
ATTTTAGGAGTACAGCTGTTTAAAGGAAAGTTTTATTATTGTGATGGTCC
CCATTTGCAAGCAGTGAAAACTAAAAACGATTGCTTGAAGTATAGTCAAA
ATGAATGGAAAAATCAGAAATACAATTTCGACAATCTGGGACAAGCATTA
ATGGCTTTATTTGTATTGTCTTCCAAAGATGGATGGGTTCAAATAATGTA
TACTGGAATCGACGCTGTTGGTGTAAATATTCAACCTCAAGAAAATCACA
ATGAATGGGCACTAATATACTTCATATCTTTTTTACTCTTAGTGGCGTTT
TTTGTTTTAAATATGTTTGTTGGAGTTGTAGTGGAAAATTTTCACAAGTG
TCAGGAAAATCAAGAGAAAGAAGAAAAGGCAAGACGTGCAGCAAAACGAG
CTAGACGCTTGGAGAAAAAACGAAAACATCTTCGAGCACCACTATATTGG
GAAAACTATTCAAAATGGCGGAGAACAATAAATTCAGTCGTTACCAGTAA
ATATTTTGATTTAGCAATCGCAGGGGTCATCGGGTTGAATGTCATAACAA
TGGCCACAGAATTCCACATGATGCCCCAGGTTTTAAAAACAGCACTGAAA
GTTTTCAATTACTTTTTTACCTCAACATTCGTTCTTGAAGCCCTGCTTAA
ATTAGTCGCTTTGGGACTTGCACGATATATTAGCGACAAATGGAATCAGT
TGGACATGATGATCGTCATTTTGTCTATTGCAGGAGTCGTTATAGATGAA
GTTGAAAGCAATTTCATGCCAATTAATCCAACTATTATGCGAGTAATGAG
AGTTTTGAGAATAGCTAGAGTATTGAAATTGCTGAAAATGGCAAGTGGAA
TCAGAGCTTTATTGGACACTGTAATTCAAGCTCTTCCTCAAGTAGGAAAT
TTAGGATTACTTTTCTTTCTATTATTTTATATTTTTGCTGCACTTGGTGT
GGAATTGTTTGGCAGATTGGAATGTTCTGATATAAATCCTTGTGTTGGAC
TAGGAAGACATGCTCATTTTGAAAATTTTGGTATGGCATTTTTAACGTTG
TTTCGAATTGCAACTGGCGATAATTGGAATGGAATAATGAAAGATACACT
CAGGGAAAAATGTATATCATCCGAAGACTGTCAGAGAAATTGCTGTGTTT
CAGCTATTATTGCTCCCATTTATTTTGTAATTTTTGTTCTCATGGCTCAA
TTTGTTTTAGTCAATGTTGTTGTGGCCGTATTAATGAAACATTTAGAAGA
GTCTCATAAAATGATGTGTGATGATTATGAATTAGAGCGTGAAGTTCAGC
GTGATCTTGACGAAGAGGCAAAAGAATTGGCAAGAGAAAAACAATTGAAA
GACGAAGAGAATAATGAAAATACGCTCATTGACATCGAAATAGACAAAAA
TAATAATTTTCTTGATAAAAAAGAAAATAACTCCATAAATCAAAATCAGA
TCAAAAAGTTATCCTGCGGAGACTGCCATTCAATTAATGCACAGTTGAGA
AATGTTTCATGTCAGAATGTTCATTTGGTCAATATCTACAGTAAAAATCT
TTCACGCAGTCATCCGCATTTGTTTGTAAATCTTGATTCGGCTTCTTTTA
CTAATAATACCAAAACAACCAATTTAAACAAATTATTACAGCGAAGACTA
TCTATGAAGAAAGAGAGTTGGAATAATGAATCAGTTATGTTCAATAATAA
TAAACCAATGAAAATAAGTGAAAATCCGAAGAGTCTAAAATCCAAAATTA
ATAAGTCTCAACAATCAACCACCGAATTGAGGCTGTCGGAAAAGAGGAAA
TTATTTGCAAAAAGTAAAATCAACGAAAAGGAAATTGAGCAAGATCCGTG
AAAAAGAAGGGAGAGAGTACGTATCAGATTTATAATGACAGTTCTGAAAA
AGGAAATTCATTTTGTGCCTGATAAATAATTGTAATAAAAGAATTTCTAA
GCCGA
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protein sequence of SMED30018324-orf-1

>SMED30018324-orf-1 ID=SMED30018324-orf-1|Name=SMED30018324-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2050bp
MKKSENKVERKKYSLDSFPSSVFYEANEFLEIENEIECLDILPQDNRSRR
ETLIGNKSDFNLTDKSKADHCQQETDGTPINPNESLIYHGFYATVFHIFH
QNKHPRDWCLKIITWPWFEQLSMTIIAVNCITLSMYQPCGDQNCETSKCQ
IMQAFDYIIFAFFTCEMVIKIIAMGFLGDLGYFGDTWNRLDMFIVIVGSI
EILPSTGNNIGLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNV
LLLCFFVFFIFGIIGVQMWQGVLRNRCYLNLNESFRHPGLNLSSFYQINR
TLFLNAVDKDFICSTDKNNGILKCSDIPPSIMLLSNNSLQICKQPARPFT
DNLPTKHSCVNWNQYYTDCQPGEKNPYEGAINFDNIGFAWVAIFQVISME
SWVDIMYYVQDAHSFWDWIYFVALIIIGSFFMINLCLVVIASQFSETKRR
ETERMWIERRRFNSSNCSSISKDEPQTCYLEIIKLITQIICKIYRRIVKL
YNQIQIRHSKTAQGNYQNKLKRQRKHCNVCLNSRSQFPIDDHCHLYSIVN
TISGDKQHYYAINKYPINFSNIPPKASPELSVIDVLNSPRHPNHLISDIS
SKSVSTETKRKSQYNSQILLEIPSTPANHSTTRDNYRWELTNNPLLTSKD
KVYPQSVASRSSSINSCAYQIPRLTDSYSKHRRPFNKNQNKTKRKNYLSI
CQQKIKTLVNDVKFQRGILIAILINTLSMGIEYHQQPVQLTQTLEFSNIV
FSILFLIEMILKLIGEGILGYIKNGFNLFDGIIVVLGVIELCQQGGTSGL
SILRTFRLLRILKLVRFMPALRQQLFVMIRTIDNVATFFALLILFIFIFS
ILGMSLFGCKFCTIENNKRTCDRKNFDSLLWSIVTVFQILTQEDWNTVLY
NGMTRTSSWAALYFIALMTFGNYVLFNLLVAILVEGFSSERLDEQKLLHN
YRQSLKKRRANNKQLRIMQHQSENLVLLTNSIQKDQTGENQKISSQPPVI
IHTAATPMPTPRSSPYNELVTTPHTTFPHTNTSNSQSILSINKSFETTSS
RNINTTILQEDASIMPGMKNSKQNLDSTLFVEHHIPVQKTPTSNGLNVKL
VSIPIPYTTNRSNFIIPSSILLRQGSINSFMGSVNSDRSKILLDEKKSIS
ETDEKSLSRQSSFKSFMAYKNKINQLTIPFSNRYSLSLDSEGKSNYQFKM
NYLLAREKALEEPDDSNSESQNSSRMNNVEDPDEEDMEAMICLSKCCPQP
KGIFAKRYYHSLYVFSVDNQFRQKCQHIVDKKSFDYLVLFFITLNCITLA
MERPSIPPHSKERLFLTVTNYIFTIIFTGEMIIKVLSKGLLIGDQAYLKS
GWNIMDGFLVFISLLDLFITILLGHSNRIFGILRVFRLLRTLRPLRVISR
APGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGKFYYCDGP
HLQAVKTKNDCLKYSQNEWKNQKYNFDNLGQALMALFVLSSKDGWVQIMY
TGIDAVGVNIQPQENHNEWALIYFISFLLLVAFFVLNMFVGVVVENFHKC
QENQEKEEKARRAAKRARRLEKKRKHLRAPLYWENYSKWRRTINSVVTSK
YFDLAIAGVIGLNVITMATEFHMMPQVLKTALKVFNYFFTSTFVLEALLK
LVALGLARYISDKWNQLDMMIVILSIAGVVIDEVESNFMPINPTIMRVMR
VLRIARVLKLLKMASGIRALLDTVIQALPQVGNLGLLFFLLFYIFAALGV
ELFGRLECSDINPCVGLGRHAHFENFGMAFLTLFRIATGDNWNGIMKDTL
REKCISSEDCQRNCCVSAIIAPIYFVIFVLMAQFVLVNVVVAVLMKHLEE
SHKMMCDDYELEREVQRDLDEEAKELAREKQLKDEENNENTLIDIEIDKN
NNFLDKKENNSINQNQIKKLSCGDCHSINAQLRNVSCQNVHLVNIYSKNL
SRSHPHLFVNLDSASFTNNTKTTNLNKLLQRRLSMKKESWNNESVMFNNN
KPMKISENPKSLKSKINKSQQSTTELRLSEKRKLFAKSKINEKEIEQDP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0005261cation channel activity
GO:0005245voltage-gated calcium channel activity
GO:0005216ion channel activity
GO:0005244voltage-gated ion channel activity
GO:0005248voltage-gated sodium channel activity
GO:0005262calcium channel activity
GO:0008332low voltage-gated calcium channel activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR043203VGCC_Ca_Na
IPR005445VDCC_T_a1
IPR002077VDCCAlpha1
IPR005821Ion_trans_dom
IPR027359Volt_channel_dom_sf
IPR001156Transferrin-like_dom
Vocabulary: biological process
TermDefinition
GO:0070588calcium ion transmembrane transport
GO:0006811ion transport
GO:0055085transmembrane transport
GO:0006816calcium ion transport
GO:0019228neuronal action potential
GO:0034765regulation of ion transmembrane transport
GO:0035725sodium ion transmembrane transport
GO:0060078regulation of postsynaptic membrane potential
GO:0070509calcium ion import
GO:0086010membrane depolarization during action potential
Vocabulary: cellular component
TermDefinition
GO:0005891voltage-gated calcium channel complex
GO:0016020membrane
GO:0005886plasma membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1859..1889
NoneNo IPR availableCOILSCoilCoilcoord: 1550..1573
NoneNo IPR availableGENE3DG3DSA:1.10.287.70coord: 1403..1552
e-value: 4.0E-25
score: 89.8
coord: 1716..1853
e-value: 3.5E-30
score: 106.1
coord: 364..449
e-value: 1.1E-16
score: 62.7
NoneNo IPR availableGENE3DG3DSA:1.10.287.70coord: 821..936
e-value: 6.3E-37
score: 127.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 2025..2049
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1211..1231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1993..2024
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1993..2049
NoneNo IPR availablePANTHERPTHR10037:SF192VOLTAGE-DEPENDENT T-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1Hcoord: 121..2027
NoneNo IPR availableSUPERFAMILYSSF81324Voltage-gated potassium channelscoord: 1281..1551
NoneNo IPR availableSUPERFAMILYSSF81324Voltage-gated potassium channelscoord: 1599..1852
NoneNo IPR availableSUPERFAMILYSSF81324Voltage-gated potassium channelscoord: 714..938
NoneNo IPR availableSUPERFAMILYSSF81324Voltage-gated potassium channelscoord: 115..447
NoneNo IPR availableTMHMMTMhelixcoord: 1815..1837
NoneNo IPR availableTMHMMTMhelixcoord: 418..440
NoneNo IPR availableTMHMMTMhelixcoord: 386..408
NoneNo IPR availableTMHMMTMhelixcoord: 158..177
NoneNo IPR availableTMHMMTMhelixcoord: 835..857
NoneNo IPR availableTMHMMTMhelixcoord: 1522..1544
NoneNo IPR availableTMHMMTMhelixcoord: 1418..1440
NoneNo IPR availableTMHMMTMhelixcoord: 1734..1756
NoneNo IPR availableTMHMMTMhelixcoord: 781..803
NoneNo IPR availableTMHMMTMhelixcoord: 749..771
NoneNo IPR availableTMHMMTMhelixcoord: 1638..1660
NoneNo IPR availableTMHMMTMhelixcoord: 1321..1343
NoneNo IPR availableTMHMMTMhelixcoord: 1358..1380
NoneNo IPR availableTMHMMTMhelixcoord: 244..266
NoneNo IPR availableTMHMMTMhelixcoord: 1596..1618
NoneNo IPR availableTMHMMTMhelixcoord: 1776..1795
NoneNo IPR availableTMHMMTMhelixcoord: 909..931
IPR002077Voltage-dependent calcium channel, alpha-1 subunitPRINTSPR00167CACHANNELcoord: 1474..1486
score: 41.11
coord: 874..900
score: 59.14
IPR005445Voltage-dependent calcium channel, T-type, alpha-1 subunitPRINTSPR01629TVDCCALPHA1coord: 96..112
score: 41.18
coord: 1555..1570
score: 61.98
coord: 474..488
score: 40.0
coord: 730..743
score: 59.52
coord: 138..154
score: 43.14
IPR027359Voltage-dependent channel domain superfamilyGENE3DG3DSA:1.20.120.350coord: 1590..1714
e-value: 9.3E-33
score: 114.8
coord: 702..820
e-value: 7.8E-30
score: 105.3
coord: 1271..1402
e-value: 2.0E-34
score: 120.2
IPR027359Voltage-dependent channel domain superfamilyGENE3DG3DSA:1.20.120.350coord: 108..231
e-value: 6.8E-27
score: 96.0
IPR005821Ion transport domainPFAMPF00520Ion_transcoord: 116..451
e-value: 6.9E-72
score: 241.6
coord: 1282..1554
e-value: 6.9E-65
score: 218.7
coord: 716..941
e-value: 6.0E-49
score: 166.5
coord: 1600..1852
e-value: 1.7E-52
score: 178.1
IPR043203Voltage-gated cation channel calcium and sodiumPANTHERPTHR10037VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUMcoord: 121..2027