SMED30018291

Overview
NameSMED30018291
Smed IDSMED30018291
Length (bp)654
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018291

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 29

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30018291h1SMcG0005835 Contig7058uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018291h1SMcG0005835 Contig7058newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018291h1SMcG0005836 Contig7058uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018291h1SMcG0005836 Contig7058newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30018291 SmedASXL_012879SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018291h1SMcG0005823 Contig19411uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018291h1SMcG0005823 Contig19411newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018291h1SMcG0005817 Contig10395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018291h1SMcG0005817 Contig10395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018291h1SMcG0005834 Contig10395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018291h1SMcG0005834 Contig10395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymaSMED30018291 dd_Smed_v4_23695_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30018291h1SMcG0005817 dd_Smed_v6_16570_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30018291h1SMcG0005823 OX_Smed_1.0.12815ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30018291h1SMcG0005817 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30018291h1SMcG0005823 dd_Smed_v4_20102_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018291h1SMcG0005823 dd_Smed_v4_5097_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018291 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.012180:7142..7801 -3167
v31.005332:38306..38853 -2280
v31.002942:1764..3139 -2138
v31.005383:51610..52262 -779
v31.012180:7373..8057 -696
v31.002942:2721..3360 +688
Homology
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BLAST of SMED30018291 vs. Planmine SMEST
Match: SMESG000047799.1 (SMESG000047799.1)

HSP 1 Score: 340.887 bits (873), Expect = 2.672e-114
Identity = 211/220 (95.91%), Postives = 215/220 (97.73%), Query Frame = 1 
Query:    1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIXXXXXXXXXXXXXNKDNECKISQLETPNKYQTNINQRPYSAEPTGQLQNENARVKSKNEEITIDCQSKMNDXXXXXXXXXXXXXXXXXKCTNLQSGNYQLESELKE 654
            LLSE+DNLKFL+KE+DDKNKILQNNYNKS EEN  LK QRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIEKLKLELDRLELKNKDNECKISQLETPNKYQTNINQRPYSAEPTGQLQNENARVKSKNEEITIDCQSKMNDLNNCINNLILELNQLEEKCTNLQSGNYQLESELKE
Sbjct:  381 LLSERDNLKFLVKEIDDKNKILQNNYNKSGEENENLKIQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIEKLKLELDRLELKNKDNECKISQLETPNKYQTNINQRPYSAEPTGQLQNENARVKSKNEEITIDCQSKMNDLNNCINNLILELNQLEEKCTNLQSGNYQLESELKE 600

HSP 2 Score: 81.2629 bits (199), Expect = 1.140e-17
Identity = 42/66 (63.64%), Postives = 53/66 (80.30%), Query Frame = 1 
Query:  139 KLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSI 336
            +LES LKES     +LHKD DDCKAELQRS ERN Q +NE+++NKRKIEEI KD+QLK++N  N++
Sbjct:  593 QLESELKESDKIKNKLHKDYDDCKAELQRSAERNLQLENEMEINKRKIEEIKKDFQLKLDNLFNTV 658

HSP 3 Score: 65.0846 bits (157), Expect = 3.905e-12
Identity = 43/103 (41.75%), Postives = 60/103 (58.25%), Query Frame = 1 
Query:   82 KSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEI-------------------DVNKRKIEEITKDYQLKMENSNNS 333
            K++ E  K +  + + +IN+LES LKE    N RL +D DDCK +L +S+ RNSQ ++EI                   D +KRK EEI+KD+QLKM+NS  S
Sbjct:  201 KTQLEKKKTRVKRQIEKINQLESELKELEGINLRLQQDFDDCKVKLNQSQRRNSQLESEINKKQCENCAELIKQLQKQKDTSKRKTEEISKDFQLKMDNSKTS 303
[Close]
BLAST of SMED30018291 vs. Planmine SMEST
Match: SMESG000038528.1 (SMESG000038528.1)

HSP 1 Score: 241.506 bits (615), Expect = 2.655e-74
Identity = 148/176 (84.09%), Postives = 160/176 (90.91%), Query Frame = 1 
Query:  130 EINKLES-LLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSIXXXXXXXXXXXXXNKDNECKISQLETPNKYQTNINQRPYSAEPTGQLQNENARVKSKNEEITIDCQSKMNDXXXXXXXXXXXXXXXXXKCTNLQSGNYQLESELKE 654
            E+N+L++ +LKESYATN RLH+DCDDCKAELQRSEE+NSQFKNEIDVNKRKIEEITKDYQLKMENSNNSI     E  +LELKNKDNECKISQLET NKYQTNIN+RPYSAE TGQLQNENARVKSKNEE TIDCQSKMNDLN  +NNLIL+LNQ EE+ TNLQSGNYQLESELKE
Sbjct:  686 ELNQLQNKMLKESYATNTRLHQDCDDCKAELQRSEEKNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSI-----EKLKLELKNKDNECKISQLETANKYQTNINKRPYSAESTGQLQNENARVKSKNEEFTIDCQSKMNDLNKSMNNLILKLNQSEEERTNLQSGNYQLESELKE 856

HSP 2 Score: 117.472 bits (293), Expect = 3.988e-30
Identity = 74/135 (54.81%), Postives = 93/135 (68.89%), Query Frame = 1 
Query:    1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDD----------------CKAELQRSEERNS-----QFKNEIDVNKRKIEEITKDYQLKMENSNNSI 336
            L S++DNLK L+KE+DDKNKILQNNYNKS EEN  LKFQ DKYLAEIN+LES+LKES  TNRRL ++ D                  K+++ R+   N+     Q +NE + +KRK+EEITKD+QLKME+ N SI
Sbjct:  444 LQSDRDNLKLLVKEIDDKNKILQNNYNKSGEENENLKFQCDKYLAEINRLESVLKESDRTNRRLQQEFDAKSKHSDRNSQLESTNLFKSQMDRNRFENNAELIKQLENEKETDKRKVEEITKDFQLKMEDLNRSI 578

HSP 3 Score: 83.5741 bits (205), Expect = 1.797e-18
Identity = 52/101 (51.49%), Postives = 66/101 (65.35%), Query Frame = 1 
Query:   43 MDDKNKILQN---NYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNSI 336
            M+D NK + N     N+SEEE    Q   Y     +LES LKE      +LHKD DDCKAELQRS ERN Q +NE+++NKRK+EEI KD+QLK+ N  N++
Sbjct:  819 MNDLNKSMNNLILKLNQSEEERTNLQSGNY-----QLESELKELDKIKNKLHKDYDDCKAELQRSAERNLQLENEMEINKRKLEEIKKDFQLKLHNLFNTV 914

HSP 4 Score: 68.9366 bits (167), Expect = 2.450e-13
Identity = 43/99 (43.43%), Postives = 58/99 (58.59%), Query Frame = 1 
Query:  115 DKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQF----KNEIDVN---------------------KRKIEEITKDYQLKMENSNNSI 336
            ++YLA+IN+L+S LKES   N RL +D DDCK +L  S+ RNSQ     K E  +N                     K+KIEE+ KD+QLKME+SN++I
Sbjct:  260 NQYLAQINQLQSALKESKGINLRLQQDFDDCKVKLNHSQRRNSQLESANKYEHQINRDQCANCAESTRQLQNQKSRVKKKIEELAKDFQLKMEDSNSTI 358

HSP 5 Score: 54.6842 bits (130), Expect = 1.346e-8
Identity = 60/159 (37.74%), Postives = 78/159 (49.06%), Query Frame = 1 
Query:  121 YLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRK---IEEITKDYQLKMENSNNSIXXXXXXXXXXXXXNKDNECKISQLET---PNKYQTNIN------------QRPYSAEPTGQLQNENARVKSKNEEITIDCQSKM 543
            + A+I +L+S LK+S    R L +D +DCK +L+ S+ERNSQ  NE +  KRK   IEE  K  QLKM N N S              NK+ + +++QL+       Y TN              QR  S E   Q +NE    K K EEIT D Q KM
Sbjct:  606 FTAQIEQLQSELKKSDRKMRILQRDFEDCKGKLKHSQERNSQLVNENETVKRKSHQIEESDKGCQLKMMNLNTS--------------NKNLKLELNQLQNKMLKESYATNTRLHQDCDDCKAELQR--SEEKNSQFKNEIDVNKRKIEEITKDYQLKM 748
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BLAST of SMED30018291 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1)

HSP 1 Score: 174.481 bits (441), Expect = 3.727e-50
Identity = 91/113 (80.53%), Postives = 104/113 (92.04%), Query Frame = 1 
Query:    1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNS 333
            LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN  LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQ +NEI+VN RK +EI K +Q+++++SN S
Sbjct:  428 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYS 540

HSP 2 Score: 73.559 bits (179), Expect = 6.144e-15
Identity = 47/104 (45.19%), Postives = 69/104 (66.35%), Query Frame = 1 
Query:   22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNS 333
            LK  +K+ ++K + LQN+ N  E         ++  +I +L+S LKES      L +D +DCK +L+ S+ERN Q +NE+ VNKR IEEIT+D+QLKME+SN+S
Sbjct:  544 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSS 639

HSP 3 Score: 46.2098 bits (108), Expect = 8.641e-6
Identity = 37/123 (30.08%), Postives = 62/123 (50.41%), Query Frame = 1 
Query:   31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERN---------------------SQFKNEIDVNKRKIEEITKDYQLKMENSNNSI 336
            LIK++  +N   +  Y K + EN     ++YLA+I +L+S  +ES  TN+RL  + D  K +L+RS+ERN                     ++  N++   K K   I KD + +++N N S+
Sbjct:  188 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSV 305
[Close]
BLAST of SMED30018291 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1)

HSP 1 Score: 174.481 bits (441), Expect = 4.075e-50
Identity = 91/113 (80.53%), Postives = 104/113 (92.04%), Query Frame = 1 
Query:    1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNS 333
            LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN  LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQ +NEI+VN RK +EI K +Q+++++SN S
Sbjct:  423 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYS 535

HSP 2 Score: 73.559 bits (179), Expect = 6.314e-15
Identity = 47/104 (45.19%), Postives = 69/104 (66.35%), Query Frame = 1 
Query:   22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNS 333
            LK  +K+ ++K + LQN+ N  E         ++  +I +L+S LKES      L +D +DCK +L+ S+ERN Q +NE+ VNKR IEEIT+D+QLKME+SN+S
Sbjct:  539 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSS 634

HSP 3 Score: 46.2098 bits (108), Expect = 8.956e-6
Identity = 37/123 (30.08%), Postives = 62/123 (50.41%), Query Frame = 1 
Query:   31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERN---------------------SQFKNEIDVNKRKIEEITKDYQLKMENSNNSI 336
            LIK++  +N   +  Y K + EN     ++YLA+I +L+S  +ES  TN+RL  + D  K +L+RS+ERN                     ++  N++   K K   I KD + +++N N S+
Sbjct:  183 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSV 300
[Close]
BLAST of SMED30018291 vs. Planmine SMEST
Match: SMESG000036067.1 (SMESG000036067.1)

HSP 1 Score: 174.096 bits (440), Expect = 7.354e-50
Identity = 91/113 (80.53%), Postives = 104/113 (92.04%), Query Frame = 1 
Query:    1 LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEEN--LKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNS 333
            LLSE+DNLKFL+KE+DDKNKILQNNYNKSEEEN  LKFQRDKYLAEINKLES+LKESY TNRRLHKDCDDCKAELQRSEERNSQ +NEI+VN RK +EI K +Q+++++SN S
Sbjct:  423 LLSERDNLKFLVKEIDDKNKILQNNYNKSEEENENLKFQRDKYLAEINKLESVLKESYTTNRRLHKDCDDCKAELQRSEERNSQLENEIEVNNRKNKEIAKYFQMQIKDSNYS 535

HSP 2 Score: 73.1738 bits (178), Expect = 8.173e-15
Identity = 47/104 (45.19%), Postives = 69/104 (66.35%), Query Frame = 1 
Query:   22 LKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKDYQLKMENSNNS 333
            LK  +K+ ++K + LQN+ N  E         ++  +I +L+S LKES      L +D +DCK +L+ S+ERN Q +NE+ VNKR IEEIT+D+QLKME+SN+S
Sbjct:  539 LKLELKQSENKFQSLQNDNNNIE--------SRFAEQIEQLQSALKESDRKMGSLQRDFEDCKEKLKHSQERNLQLENELVVNKRTIEEITEDFQLKMEDSNSS 634

HSP 3 Score: 46.2098 bits (108), Expect = 8.635e-6
Identity = 37/123 (30.08%), Postives = 62/123 (50.41%), Query Frame = 1 
Query:   31 LIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLESLLKESYATNRRLHKDCDDCKAELQRSEERN---------------------SQFKNEIDVNKRKIEEITKDYQLKMENSNNSI 336
            LIK++  +N   +  Y K + EN     ++YLA+I +L+S  +ES  TN+RL  + D  K +L+RS+ERN                     ++  N++   K K   I KD + +++N N S+
Sbjct:  183 LIKQLQKENSQFEGKYKKLQNEN-----NQYLAQIERLKSGFRESERTNKRLQLNIDQYKTKLERSQERNLKLESANRLKSQMERNRCVNCAELINQLQNEKGKTNRIAKDCEFQLDNLNRSV 300
The following BLAST results are available for this feature:
BLAST of SMED30018291 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018291 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000047799.12.672e-11495.91SMESG000047799.1[more]
SMESG000038528.12.655e-7484.09SMESG000038528.1[more]
SMESG000036067.13.727e-5080.53SMESG000036067.1[more]
SMESG000036067.14.075e-5080.53SMESG000036067.1[more]
SMESG000036067.17.354e-5080.53SMESG000036067.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018291 ID=SMED30018291|Name=SMED30018291|organism=Schmidtea mediterranea sexual|type=transcript|length=654bp
TTGCTGTCAGAAAAGGATAATTTGAAATTTCTAATAAAAGAAATGGATGA
TAAAAATAAAATTCTGCAAAATAATTATAATAAATCAGAAGAAGAAAATT
TAAAATTTCAACGTGATAAATATTTGGCAGAGATTAATAAATTGGAATCA
TTGTTGAAGGAATCATACGCGACAAATAGGAGATTACATAAGGATTGTGA
CGATTGCAAAGCGGAATTGCAACGTTCAGAGGAGAGAAATTCACAATTCA
AAAATGAAATTGATGTAAATAAAAGAAAAATTGAAGAAATCACAAAAGAT
TATCAGTTGAAAATGGAGAATTCAAATAATTCCATTGAGAAGCTGAAATT
GGAATTGGATCGATTGGAATTGAAAAACAAAGATAATGAGTGCAAAATTT
CACAATTGGAAACACCAAATAAATATCAAACTAACATAAATCAACGTCCG
TATTCTGCAGAACCCACAGGACAATTGCAAAATGAAAACGCGAGAGTTAA
AAGTAAAAATGAAGAAATTACAATTGATTGCCAATCGAAAATGAATGACT
TAAATAATTGTATTAATAATCTGATATTGGAATTGAATCAATTGGAAGAG
AAATGCACAAACTTGCAGAGTGGAAATTATCAATTGGAATCAGAATTGAA
GGAA
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protein sequence of SMED30018291-orf-1

>SMED30018291-orf-1 ID=SMED30018291-orf-1|Name=SMED30018291-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=218bp
LLSEKDNLKFLIKEMDDKNKILQNNYNKSEEENLKFQRDKYLAEINKLES
LLKESYATNRRLHKDCDDCKAELQRSEERNSQFKNEIDVNKRKIEEITKD
YQLKMENSNNSIEKLKLELDRLELKNKDNECKISQLETPNKYQTNINQRP
YSAEPTGQLQNENARVKSKNEEITIDCQSKMNDLNNCINNLILELNQLEE
KCTNLQSGNYQLESELKE
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 19..53
NoneNo IPR availableCOILSCoilCoilcoord: 61..134
NoneNo IPR availableCOILSCoilCoilcoord: 183..217
NoneNo IPR availableSUPERFAMILYSSF90257Myosin rod fragmentscoord: 170..220