Calbindin-32
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30018274 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 17
Homology
BLAST of Calbindin-32 vs. Ensembl Human
Match: SCGN (secretagogin, EF-hand calcium binding protein [Source:HGNC Symbol;Acc:HGNC:16941]) HSP 1 Score: 210.305 bits (534), Expect = 2.179e-65 Identity = 123/271 (45.39%), Postives = 169/271 (62.36%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQV-LPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITT----VDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
L A F +VW +DAD GYIE ELD+F ++ LG D D + K L +K+ F+ D + D +I M ELA + L DE FLLLFR++ PLDSSVEFM+IW+++D D SGFI A EL+NFL + + + ISE KL EYT TM+++FD NKDG+L L+++AR+L ++EN+L + FK D +T D E +F YD K G +E E+ GF+KD++ LVQ DL+ FR+I+L DVN DGKI +EL + L
Sbjct: 9 LGRLDAAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMK-LGTD---DTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLLLFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHK--KAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQ--FK-MDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 270 HSP 2 Score: 89.7373 bits (221), Expect = 8.654e-20 Identity = 53/178 (29.78%), Postives = 100/178 (56.18%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
++ +F ++W YDAD +G+I +EL +FL + + + +++ L + + FD+N D ++++++LA++L E FLL F+ DA + +F KI+ +D ++G +E E+ F+ + ++ +P IS L ++ +L+ D NKDGK+Q SE+A L +K N
Sbjct: 106 SSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLH------HKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQP--SISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 275 HSP 3 Score: 75.485 bits (184), Expect = 8.442e-15 Identity = 46/170 (27.06%), Postives = 93/170 (54.71%), Query Frame = 3
Query: 450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+ F ++W++FD D+ G+IE EL F L + ++ + + L + + D++KDG++++ E+A + L EN+L +F+ + + ++V+ ++ YD D +G I EL+ FL+DL ++ + LE + ++ +D N DG++ +N+L +L Q
Sbjct: 14 AAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMKLGTDDTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLL--LFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQ 181
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 209.92 bits (533), Expect = 3.347e-65 Identity = 118/273 (43.22%), Postives = 178/273 (65.20%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
L TA+QF E+W H+DADGNGYIEG EL++F E + G + N + ++F+ +D+NSD KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+T+ + +F YD+D++G I+ EL LKDL +++ + L +R+ +++ + GK+ +L+++L + +
Sbjct: 13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMS---LAEAGKLYRKDLEIVLCSEPPM 271
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 209.92 bits (533), Expect = 3.347e-65 Identity = 118/273 (43.22%), Postives = 178/273 (65.20%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
L TA+QF E+W H+DADGNGYIEG EL++F E + G + N + ++F+ +D+NSD KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+T+ + +F YD+D++G I+ EL LKDL +++ + L +R+ +++ + GK+ +L+++L + +
Sbjct: 13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMS---LAEAGKLYRKDLEIVLCSEPPM 271
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 193.741 bits (491), Expect = 1.948e-59 Identity = 107/231 (46.32%), Postives = 155/231 (67.10%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ 836
L TA+QF E+W H+DADGNGYIEG EL++F E + G + N + ++F+ +D+NSD KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+T+ + +F YD++ N ++L + K +++L +
Sbjct: 13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKEMN----IQQLTNYRKSVMSLAE 228 HSP 2 Score: 110.923 bits (276), Expect = 7.565e-28 Identity = 62/174 (35.63%), Postives = 104/174 (59.77%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE-----EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
++ +F++IWK FD D +G+IE EL+NF E K + +S+ EK+ E+ +Q +D N DGK++++E+A++LP +EN+L F+ ++ + + YD D++G IE EL+GFL DLL YD+ L+ + Q IL +D+N DGK+ ++E+ +L Q
Sbjct: 17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEF----MQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 193.741 bits (491), Expect = 1.948e-59 Identity = 107/231 (46.32%), Postives = 155/231 (67.10%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ 836
L TA+QF E+W H+DADGNGYIEG EL++F E + G + N + ++F+ +D+NSD KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+T+ + +F YD++ N ++L + K +++L +
Sbjct: 13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKEMN----IQQLTNYRKSVMSLAE 228 HSP 2 Score: 110.923 bits (276), Expect = 7.565e-28 Identity = 62/174 (35.63%), Postives = 104/174 (59.77%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE-----EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
++ +F++IWK FD D +G+IE EL+NF E K + +S+ EK+ E+ +Q +D N DGK++++E+A++LP +EN+L F+ ++ + + YD D++G IE EL+GFL DLL YD+ L+ + Q IL +D+N DGK+ ++E+ +L Q
Sbjct: 17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEF----MQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183
BLAST of Calbindin-32 vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305]) HSP 1 Score: 49.2914 bits (116), Expect = 4.801e-7 Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query: 168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+F E +S +D DG+G I EL + ++ LG++ EL +D ++ D + + I+ E ++ RK DS E + ++ FDKD +GFI A EL++ ++ L E +E++ E M++ D + DG++ E ++ K
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTTK 149
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0340072) HSP 1 Score: 347.436 bits (890), Expect = 8.141e-119 Identity = 170/295 (57.63%), Postives = 223/295 (75.59%), Query Frame = 3
Query: 84 KMSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALV-QEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
++ K NF+ QY+D E+++L+ +A QF +VW+HYD DGNGYIEG+ELD FL EF+ S DI+P+ + L +LK F++A+D N D KI++ ELAQ+LP +E FLLLFR D PL+SSVEFMKIW+++D D SG+IEADELKNFL + LKEA+ +SE+KLIEYT TMLQ+FD+NKDG+LQLSEMA+LLPVKEN+LCR +FK A K+T DIE VF LYDRD +GTIENEEL+GFLKDLL LV ++DYD DL F + I+ + GKIS EL M+L+ ++++
Sbjct: 11 RVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMILLTLAKIS 305
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0087056) HSP 1 Score: 347.436 bits (890), Expect = 8.141e-119 Identity = 170/295 (57.63%), Postives = 223/295 (75.59%), Query Frame = 3
Query: 84 KMSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALV-QEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
++ K NF+ QY+D E+++L+ +A QF +VW+HYD DGNGYIEG+ELD FL EF+ S DI+P+ + L +LK F++A+D N D KI++ ELAQ+LP +E FLLLFR D PL+SSVEFMKIW+++D D SG+IEADELKNFL + LKEA+ +SE+KLIEYT TMLQ+FD+NKDG+LQLSEMA+LLPVKEN+LCR +FK A K+T DIE VF LYDRD +GTIENEEL+GFLKDLL LV ++DYD DL F + I+ + GKIS EL M+L+ ++++
Sbjct: 11 RVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMILLTLAKIS 305
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304964) HSP 1 Score: 48.1358 bits (113), Expect = 1.389e-6 Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query: 168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+F E +S +D DG+G I EL + ++ LG++ EL +D ++ D + + I+ E ++ RK DS E + ++ FDKD +GFI A EL++ ++ L E +E++ E M++ D + DG++ E ++ K
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTSK 149
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0088001) HSP 1 Score: 48.1358 bits (113), Expect = 1.389e-6 Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query: 168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+F E +S +D DG+G I EL + ++ LG++ EL +D ++ D + + I+ E ++ RK DS E + ++ FDKD +GFI A EL++ ++ L E +E++ E M++ D + DG++ E ++ K
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTSK 149
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304963) HSP 1 Score: 48.1358 bits (113), Expect = 1.389e-6 Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query: 168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+F E +S +D DG+G I EL + ++ LG++ EL +D ++ D + + I+ E ++ RK DS E + ++ FDKD +GFI A EL++ ++ L E +E++ E M++ D + DG++ E ++ K
Sbjct: 12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTSK 149
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2a (calbindin 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1677]) HSP 1 Score: 218.009 bits (554), Expect = 1.284e-68 Identity = 120/272 (44.12%), Postives = 175/272 (64.34%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
L TA+QF ++W H+DADGNGYIEG ELD+F S+ ++ G ++P + +F+ FD+N D +IEMSELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SGFIEA+ELK FLS+ LK+A ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F+ +++ +F YD+D NG I+ +EL LKDL ++ D L ++Q I+ ++ GK+ +EL+++L + L
Sbjct: 13 LAELTASQFLDIWKHFDADGNGYIEGKELDNFFSQLEIARRGAGVDP---SNAAFKGRMTEFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSTEFMAAWRKYDTDRSGFIEANELKGFLSDLLKKA--NRHYDDQKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGV-RLSAEQFNAIFTYYDKDGNGYIDEQELDALLKDLYEKNNKEVDSKSLTGYKQSIMA---LSDGGKLYRSELEIVLCRDPML 271
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010]) HSP 1 Score: 214.542 bits (545), Expect = 3.184e-67 Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 3
Query: 153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
N A F ++W H+DAD NGYIEG ELD F + + + P D++ E + +K+ F+ A+D D ++++ ELA +LP +E FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELKNFL + + + ++I KL EYT M+++FD NKDG+L L+++AR+L ++EN+L + + ++ D E +F YD + G +E E+ GF+KD++ LV+ DL+ FR+ +L D+N DGKI +EL + L
Sbjct: 7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHML-----KKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHK--KKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCL 266 HSP 2 Score: 97.4413 bits (241), Expect = 8.573e-23 Identity = 56/175 (32.00%), Postives = 105/175 (60.00%), Query Frame = 3
Query: 435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
A LD++ F++IW+ FD D +G+IE EL +F LK+ +P+++I++E++ + + + +D+ DG+LQ+ E+A + LP +EN+L IF+ + +V+ ++ YD D +G I EL+ FLKDL ++ + L+ + ++ +D N DG++ +N+L +L Q
Sbjct: 6 ANLDAA-GFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQ 177 HSP 3 Score: 96.6709 bits (239), Expect = 1.905e-22 Identity = 58/176 (32.95%), Postives = 103/176 (58.52%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKE 683
+ +F ++W YDAD +GYI +EL +FL + + + I P++L++ T + +K FD+N D ++++++LA++L E FLL F+ DA ++ +F KI+ +D ++G +E E+ F+ + ++ RP IS L ++ +L D NKDGK+Q SE+A L +K
Sbjct: 103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ-HKKKIPPNKLDEYTDAMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRP--SISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010]) HSP 1 Score: 214.542 bits (545), Expect = 3.184e-67 Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 3
Query: 153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
N A F ++W H+DAD NGYIEG ELD F + + + P D++ E + +K+ F+ A+D D ++++ ELA +LP +E FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELKNFL + + + ++I KL EYT M+++FD NKDG+L L+++AR+L ++EN+L + + ++ D E +F YD + G +E E+ GF+KD++ LV+ DL+ FR+ +L D+N DGKI +EL + L
Sbjct: 7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHML-----KKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHK--KKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCL 266 HSP 2 Score: 97.4413 bits (241), Expect = 8.573e-23 Identity = 56/175 (32.00%), Postives = 105/175 (60.00%), Query Frame = 3
Query: 435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
A LD++ F++IW+ FD D +G+IE EL +F LK+ +P+++I++E++ + + + +D+ DG+LQ+ E+A + LP +EN+L IF+ + +V+ ++ YD D +G I EL+ FLKDL ++ + L+ + ++ +D N DG++ +N+L +L Q
Sbjct: 6 ANLDAA-GFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQ 177 HSP 3 Score: 96.6709 bits (239), Expect = 1.905e-22 Identity = 58/176 (32.95%), Postives = 103/176 (58.52%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKE 683
+ +F ++W YDAD +GYI +EL +FL + + + I P++L++ T + +K FD+N D ++++++LA++L E FLL F+ DA ++ +F KI+ +D ++G +E E+ F+ + ++ RP IS L ++ +L D NKDGK+Q SE+A L +K
Sbjct: 103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ-HKKKIPPNKLDEYTDAMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRP--SISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2b (calbindin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1668]) HSP 1 Score: 201.83 bits (512), Expect = 2.857e-62 Identity = 117/268 (43.66%), Postives = 175/268 (65.30%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQ 950
L TA+QF ++W+ +DADGNG IEG EL++F+ E + I + +P + + D ++F+ FD+N D KIEMSELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SG+IE++ELK FLS+ LK+A ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F+N K++ + E +F+ YD+D NG I+ EL L+DL + D L ++ I+ ++ GK+ EL+++L +
Sbjct: 13 LAELTASQFLDIWNKFDADGNGCIEGKELENFIRE--LEIARKTADP---SNASFKDRMKEFMQKFDENGDGKIEMSELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGYIESNELKGFLSDLLKKAN--RHYDDKKLNEYTQTILKMFDLNGDGKLGLSEMARLLPVEENFLLK--FQNF-KLSAEEFEAIFNYYDKDGNGYIDEHELDALLRDLYHKHKMAVDPHSLSSSKKSIMA---LSDGGKLFRTELEIVLCK 265
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb1 (calbindin 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8107]) HSP 1 Score: 187.578 bits (475), Expect = 6.705e-57 Identity = 110/268 (41.04%), Postives = 165/268 (61.57%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKET---LSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+A+QF +++ HYD DGNG+IEG EL +F+ E + K+ L+D + F++ ++QN+D KI + EL Q+LPT+E FLL FR+ L S EFM+ W+ +D D SG+IEADELKNFL + L+ R ++ E+KL EYT T L++FDSNKDGKL LSEMARLLP EN+L + F+ K+ + +F LYD+D+NG ++ EL LKDL + + + + ++ I+ ++ GK+ EL ++L +
Sbjct: 12 SASQFLDIFHHYDNDGNGFIEGKELQNFIKEL-----------QQARKQAGLELTDKMKAFVEKYEQNADGKIGIIELVQILPTEENFLLFFRQ--QLKSCAEFMEAWRIYDADHSGYIEADELKNFLKDLLQ--RAKKPYDEKKLEEYTLTTLKIFDSNKDGKLCLSEMARLLPDNENFLLK--FQGV-KMARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNNKVLEANKISTYKSAIMA---LSDGGKLYRTELALVLCAE 258
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242]) HSP 1 Score: 216.853 bits (551), Expect = 5.436e-68 Identity = 113/263 (42.97%), Postives = 173/263 (65.78%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
A +F E+W +DAD NGYIEG ELD F + + + + + E + +K F+ A+D +D ++++ ELA +LP DE FLLLFR++ PLD+SVEFM+IW+++D+D SGFI A EL+NFL + + + +QI+ +KL EYT TM++LF+ NKDG+L L+++A++L ++EN+L + + ++ D E +F YD K G +E E+ GF+KD++ LV+ DL+ FRQI+LN DVN DGKI +EL + L
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLL-----KKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHK--KQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265 HSP 2 Score: 83.5741 bits (205), Expect = 9.465e-18 Identity = 52/170 (30.59%), Postives = 92/170 (54.12%), Query Frame = 3
Query: 450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+ EF++IW++FD D +G+IE EL F LK+ I+ EK+ + +D DG+LQ+ E+A + LP EN+L +F+ + +V+ ++ YD D +G I EL+ FL+DL ++ D L+ + ++ ++ N DG++ +N+L +L Q
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL-GANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLL--LFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQ 176 HSP 3 Score: 82.0333 bits (201), Expect = 3.249e-17 Identity = 53/177 (29.94%), Postives = 98/177 (55.37%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
+ +F +W YD D +G+I EL +FL + + + I D+L++ T + +K F++N D ++++++LA++L E FLL F+ DA + +F I+ +D ++G +E E+ F+ + ++ +P IS L ++ +L D NKDGK+Q SE+A L +K +
Sbjct: 102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ-HKKQITGDKLDEYTDTMMK-----LFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 270
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242]) HSP 1 Score: 211.46 bits (537), Expect = 7.429e-66 Identity = 111/262 (42.37%), Postives = 170/262 (64.89%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
A +F E+W +DAD NGYIEG ELD F + + + + + E + +K F+ A+D +D ++++ ELA +LP DE FLLLFR++ PLD+SVEFM+IW+++D+D SGFI A EL+NFL + + + +QI+ +KL EYT TM++LF+ NKDG+L L+++A+ + K ++L ++ ++ D E +F YD K G +E E+ GF+KD++ LV+ DL+ FRQI+LN DVN DGKI +EL + L
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLL-----KKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHK--KQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKYVFYK-DFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 263 HSP 2 Score: 68.1662 bits (165), Expect = 2.183e-12 Identity = 45/175 (25.71%), Postives = 92/175 (52.57%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
+ +F +W YD D +G+I EL +FL + + + I D+L++ T + +K F++N D ++++++LA+ + + + + + +F I+ +D ++G +E E+ F+ + ++ +P IS L ++ +L D NKDGK+Q SE+A L +K +
Sbjct: 102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ-HKKQITGDKLDEYTDTMMK-----LFNKNKDGRLDLNDLAKYVFYKDFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 268
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: gata5 (GATA binding protein 5 [Source:Xenbase;Acc:XB-GENE-484906]) HSP 1 Score: 189.504 bits (480), Expect = 3.521e-58 Identity = 95/185 (51.35%), Postives = 135/185 (72.97%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR 698
L TA+QF ++W HYD+DGNGYIEG EL++F E + G ++ +N + ++F+ +D+N+D +IEM+ELAQ+LPT+E FLL FR+ A SS+EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E+KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +
Sbjct: 12 LAELTASQFLDIWKHYDSDGNGYIEGKELENFFRELEAARRGAGVDASNVN---FGEKLKEFMQKYDKNADGRIEMAELAQILPTEENFLLCFRQHA--GSSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---FDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLK 188 HSP 2 Score: 105.531 bits (262), Expect = 3.783e-26 Identity = 61/175 (34.86%), Postives = 103/175 (58.86%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE------EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
++ +F+ IWK +D D +G+IE EL+NF E L+ AR + EKL E+ +Q +D N DG+++++E+A++LP +EN+L F+ ++++ + YD D++G IE EL+GFL DLL +D+ L + Q IL +D+N DGK+ ++E+ +L Q
Sbjct: 16 TASQFLDIWKHYDSDGNGYIEGKELENFFRE-LEAARRGAGVDASNVNFGEKLKEF----MQKYDKNADGRIEMAELAQILPTEENFLL--CFRQHAG-SSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPFDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQ 182 HSP 3 Score: 59.3066 bits (142), Expect = 1.040e-9 Identity = 33/90 (36.67%), Postives = 53/90 (58.89%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFR 428
++ +F E W YD D +GYIE +EL FLS+ +L + P +++ L + + L FD N D K+ +SE++++LP E FLL F+
Sbjct: 107 SSIEFMEAWRKYDTDRSGYIEANELKGFLSD----LLKKANRP--FDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLKFQ 190
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969]) HSP 1 Score: 187.578 bits (475), Expect = 1.113e-56 Identity = 117/259 (45.17%), Postives = 167/259 (64.48%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNEL 932
+A+QF E+W HYD DGNG+IEG EL +F+ E + ++ LSD + F+D ++ N+D KI ++ELAQ+LPT+E FLL FR+ L SS EFM+ W+++D D SGFIE DELK+FL + LK+A +P E E KL EYT TML +FD+N DGKL L+EM+ LLPV+EN+L + F+ K+ + VF LYD+D NG I+ EL LKDL ++D D ++L +++ I+ ++ GK+ EL
Sbjct: 17 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQARKKAGLD--------LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFLLFFRQQ--LKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCE---ETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLK--FQGV-KMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKDLDINNLSTYKKSIMA---LSDGGKLYRTEL 256 HSP 2 Score: 98.9821 bits (245), Expect = 2.794e-23 Identity = 57/169 (33.73%), Postives = 102/169 (60.36%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
S+ +F++IW+ +D D +GFIE EL+NF+ E L++AR + + L + + +++N DGK+ ++E+A++LP +EN+L +F ++ + + YD D +G IE +EL+ FLKDLL + ++ LE + +L +D N+DGK+ + E+ M+L Q
Sbjct: 17 SASQFLEIWRHYDTDGNGFIEGKELQNFILE-LQQARKKAGLD---LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFL---LFFRQQLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQ 178
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969]) HSP 1 Score: 181.8 bits (460), Expect = 3.898e-54 Identity = 119/287 (41.46%), Postives = 170/287 (59.23%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYL-------------------CRPIFK-----NADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
+A+QF E+W HYD DGNG+IEG EL +F+ E + ++ LSD + F+D ++ N+D KI ++ELAQ+LPT+E FLL FR+ L SS EFM+ W+++D D SGFIE DELK+FL + LK+A +P E E KL EYT TML +FD+N DGKL L+EM+ LLPV+EN+L C I + K+ + VF LYD+D NG I+ EL LKDL ++D D ++L +++ I+ ++ GK+ EL ++L
Sbjct: 12 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQARKKAGLD--------LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFLLFFRQQ--LKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCE---ETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQISVNFICVGINQMCFNPQGVKMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKDLDINNLSTYKKSIM---ALSDGGKLYRTELALVL 282 HSP 2 Score: 98.9821 bits (245), Expect = 4.544e-23 Identity = 61/186 (32.80%), Postives = 112/186 (60.22%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML-MQQSRLAKL---THLEPRQ 992
S+ +F++IW+ +D D +GFIE EL+NF+ E L++AR + + L + + +++N DGK+ ++E+A++LP +EN+L +F ++ + + YD D +G IE +EL+ FLKDLL + ++ LE + +L +D N+DGK+ + E+ M+L +Q++ L K + ++P Q
Sbjct: 12 SASQFLEIWRHYDTDGNGFIEGKELQNFILE-LQQARKKAGLD---LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFL---LFFRQQLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQ 190
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914]) HSP 1 Score: 208.764 bits (530), Expect = 6.188e-65 Identity = 118/270 (43.70%), Postives = 176/270 (65.19%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
L TA+QF E+W H+DADGNGYIEG EL++F E + G + N + ++F+ +D+NSD KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+T+ + +F YD+D +G I+ EL LKDL +++ + L +R+ +++ + GK+ +L+++L +
Sbjct: 13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTTYRKSVMS---LAEAGKLYRKDLEIVLCSE 268
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914]) HSP 1 Score: 205.297 bits (521), Expect = 5.642e-64 Identity = 112/226 (49.56%), Postives = 154/226 (68.14%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDL 821
L TA+QF E+W H+DADGNGYIEG EL++F E + G + N + ++F+ +D+NSD KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+T+ + +F YD+D +G I+ EL LKDL
Sbjct: 13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDGSGYIDENELDALLKDL 227 HSP 2 Score: 110.923 bits (276), Expect = 6.429e-28 Identity = 62/174 (35.63%), Postives = 104/174 (59.77%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE-----EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
++ +F++IWK FD D +G+IE EL+NF E K + +S+ EK+ E+ +Q +D N DGK++++E+A++LP +EN+L F+ ++ + + YD D++G IE EL+GFL DLL YD+ L+ + Q IL +D+N DGK+ ++E+ +L Q
Sbjct: 17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEF----MQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183 HSP 3 Score: 73.559 bits (179), Expect = 1.437e-14 Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFL----KEAR 557
++ +F E W YD D +GYIE +EL FLS+ +L + P ++ L + + L FD N D K+ +SE++++LP E FLL F+ +S EF I+ +DKD SG+I+ +EL L + KEAR
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSD----LLKKANRP--YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQ--GMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEAR 236
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb1 (calbindin 1 [Source:MGI Symbol;Acc:MGI:88248]) HSP 1 Score: 193.356 bits (490), Expect = 3.847e-59 Identity = 112/265 (42.26%), Postives = 167/265 (63.02%), Query Frame = 3
Query: 156 FTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSI--LGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
TA+QF E+W H+DADG+GY+EG EL + + E + + G +++P+ + F+D + Q D KI + ELA VLPT+E FLLLFR L S EFMK W+++D D SGFIE +ELKNFL + L++A + + + KL EYT ML+LFDSN DGKL+L+EMARLLPV+EN+L + F+ K+ + F LYD+D NG I+ EL LKDL +++ D +++ +++ I+ ++ GK+ +L ++L
Sbjct: 11 ITASQFFEIWLHFDADGSGYLEGKELQNLIQELLQARKKAGLELSPE----------MKSFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQ-QLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKAN--KTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLK--FQGI-KMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQELDINNITTYKKNIMA---LSDGGKLYRTDLALIL 256 HSP 2 Score: 88.1965 bits (217), Expect = 1.679e-19 Identity = 55/173 (31.79%), Postives = 94/173 (54.34%), Query Frame = 3
Query: 435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+ L ++ +F +IW FD D SG++E EL+N + E L +AR + + +L + + + DGK+ + E+A +LP +EN+L +F+ + + + YD D +G IE EEL+ FLKDLL + D L + ++L +D N+DGK+ + E+ +L Q
Sbjct: 8 SSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELL-QARKKAGL---ELSPEMKSFVDQYGQRDDGKIGIVELAHVLPTEENFLL--LFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQ 174
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873]) HSP 1 Score: 189.889 bits (481), Expect = 1.168e-57 Identity = 107/263 (40.68%), Postives = 162/263 (61.60%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQV-LPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
A F ++W +D + GYI +ELD+F +L + D L +E + +K + + + + + +I M ELA + L DE FLL FR + PLD+SVEFM+IW+++D D SGFI A EL NFL + + + ISE +L EYTSTM+++FD NKDG+L L+++AR+L ++EN+L + + ++ + D E +F YD K G +E E+ GF+KD++ LVQ DL+ FR+I+L DVN DGKI +EL + L
Sbjct: 14 AACFWQIWQRFDKEEKGYIRETELDAFFDH----LLAKSGTEDTLMEENVQKVKEQLMTSHNVSKEGRILMKELASMFLSEDENFLLFFRLETPLDNSVEFMQIWRKYDADSSGFISAAELCNFLRDLFLHHK--KNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 270 HSP 2 Score: 90.5077 bits (223), Expect = 2.839e-20 Identity = 60/180 (33.33%), Postives = 104/180 (57.78%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVE-----FMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
+ +F ++W YDAD +G+I +EL +FL + + ++I+ EL + T + +K FD+N D ++++++LA++L E FLL F+ DA SS E F KI+ +D ++G +E E+ F+ + ++ +P IS L ++ +L+ D NKDGK+Q SE+A L +K N
Sbjct: 107 SVEFMQIWRKYDADSSGFISAAELCNFLRDLFLH-HKKNISEAELEEYTSTMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDA---SSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQP--SISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 275 HSP 3 Score: 73.9442 bits (180), Expect = 1.667e-14 Identity = 49/175 (28.00%), Postives = 96/175 (54.86%), Query Frame = 3
Query: 435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
A LD++ F +IW++FDK++ G+I EL F L ++ E+ + EE + + ++ + +K+G++ + E+A + L EN+L F+ + +V+ ++ YD D +G I EL FL+DL +++ + +LE + ++ +D N DG++ +N+L +L Q
Sbjct: 10 ARLDAAC-FWQIWQRFDKEEKGYIRETELDAFFDHLLAKSGTEDTLMEENVQKVKEQLMTSHNVSKEGRILMKELASMFLSEDENFLL--FFRLETPLDNSVEFMQIWRKYDADSSGFISAAELCNFLRDLFLHHKKNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQ 181
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873]) HSP 1 Score: 88.1965 bits (217), Expect = 7.442e-21 Identity = 50/116 (43.10%), Postives = 74/116 (63.79%), Query Frame = 3
Query: 609 LQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITT----VDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
+++FD NKDG+L L+++AR+L ++EN+L + FK D +T D E +F YD K G +E E+ GF+KD++ LVQ DL+ FR+I+L DVN DGKI +EL + L
Sbjct: 1 MKIFDKNKDGRLDLNDLARILALQENFLLQ--FK-MDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 113 HSP 2 Score: 67.3958 bits (163), Expect = 1.795e-13 Identity = 43/120 (35.83%), Postives = 71/120 (59.17%), Query Frame = 3
Query: 342 FDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVE-----FMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
FD+N D ++++++LA++L E FLL F+ DA SS E F KI+ +D ++G +E E+ F+ + ++ +P IS L ++ +L+ D NKDGK+Q SE+A L +K N
Sbjct: 4 FDKNKDGRLDLNDLARILALQENFLLQFKMDA---SSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQP--SISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 118
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P41044|CAB32_DROME (Calbindin-32 OS=Drosophila melanogaster OX=7227 GN=Cbp53E PE=2 SV=1) HSP 1 Score: 347.436 bits (890), Expect = 8.160e-118 Identity = 170/295 (57.63%), Postives = 223/295 (75.59%), Query Frame = 3
Query: 84 KMSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALV-QEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
++ K NF+ QY+D E+++L+ +A QF +VW+HYD DGNGYIEG+ELD FL EF+ S DI+P+ + L +LK F++A+D N D KI++ ELAQ+LP +E FLLLFR D PL+SSVEFMKIW+++D D SG+IEADELKNFL + LKEA+ +SE+KLIEYT TMLQ+FD+NKDG+LQLSEMA+LLPVKEN+LCR +FK A K+T DIE VF LYDRD +GTIENEEL+GFLKDLL LV ++DYD DL F + I+ + GKIS EL M+L+ ++++
Sbjct: 11 RVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMILLTLAKIS 305
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q0VFG3|SEGN_XENTR (Secretagogin OS=Xenopus tropicalis OX=8364 GN=scgn PE=2 SV=1) HSP 1 Score: 216.853 bits (551), Expect = 2.880e-67 Identity = 113/263 (42.97%), Postives = 173/263 (65.78%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
A +F E+W +DAD NGYIEG ELD F + + + + + E + +K F+ A+D +D ++++ ELA +LP DE FLLLFR++ PLD+SVEFM+IW+++D+D SGFI A EL+NFL + + + +QI+ +KL EYT TM++LF+ NKDG+L L+++A++L ++EN+L + + ++ D E +F YD K G +E E+ GF+KD++ LV+ DL+ FRQI+LN DVN DGKI +EL + L
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLL-----KKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHK--KQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265 HSP 2 Score: 83.5741 bits (205), Expect = 5.014e-17 Identity = 52/170 (30.59%), Postives = 92/170 (54.12%), Query Frame = 3
Query: 450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+ EF++IW++FD D +G+IE EL F LK+ I+ EK+ + +D DG+LQ+ E+A + LP EN+L +F+ + +V+ ++ YD D +G I EL+ FL+DL ++ D L+ + ++ ++ N DG++ +N+L +L Q
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL-GANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLL--LFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQ 176 HSP 3 Score: 82.0333 bits (201), Expect = 1.721e-16 Identity = 53/177 (29.94%), Postives = 98/177 (55.37%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
+ +F +W YD D +G+I EL +FL + + + I D+L++ T + +K F++N D ++++++LA++L E FLL F+ DA + +F I+ +D ++G +E E+ F+ + ++ +P IS L ++ +L D NKDGK+Q SE+A L +K +
Sbjct: 102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ-HKKQITGDKLDEYTDTMMK-----LFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 270
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q63ZJ3|SEGN_XENLA (Secretagogin OS=Xenopus laevis OX=8355 GN=scgn PE=2 SV=1) HSP 1 Score: 215.698 bits (548), Expect = 8.194e-67 Identity = 114/263 (43.35%), Postives = 172/263 (65.40%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
A +F E+W +DAD NGYIEG ELD F + + I D++ +K F+ A+D +D ++++ ELA +LP DE FLLLFR++ PLD+SVEFM+IW++FD+D SGFI A EL+NFL + + + + I+ +KL EYT TM++LF+ NKDG+L L+++A++L ++EN+L + + ++ + D E +F YD K G +E E+ GF+KD++ LV+ DL+ FRQI+LN DVN DGKI +EL + L
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKLGTNAITADKVQG-----VKDRFMSAYDITADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKFDEDSSGFISAVELRNFLQDLFLQHK--KHITGDKLDEYTDTMMKLFNRNKDGRLDLNDLAKILALQENFLLQFKMDASSQEERKSDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265 HSP 2 Score: 82.8037 bits (203), Expect = 1.179e-16 Identity = 53/177 (29.94%), Postives = 98/177 (55.37%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
+ +F +W +D D +G+I EL +FL + + + I D+L++ T + +K F++N D ++++++LA++L E FLL F+ DA + +F I+ +D ++G +E E+ F+ + ++ +P IS L ++ +L D NKDGK+Q SE+A L +K N
Sbjct: 102 SVEFMRIWRKFDEDSSGFISAVELRNFLQDLFLQ-HKKHITGDKLDEYTDTMMK-----LFNRNKDGRLDLNDLAKILALQENFLLQFKMDASSQEERKSDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKAN 270 HSP 3 Score: 79.7221 bits (195), Expect = 1.298e-15 Identity = 50/170 (29.41%), Postives = 92/170 (54.12%), Query Frame = 3
Query: 450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+ EF++IW++FD D +G+IE EL F LK+ I+ +K+ + +D DG+LQ+ E+A + LP EN+L +F+ + +V+ ++ +D D +G I EL+ FL+DL ++ D L+ + ++ ++ N DG++ +N+L +L Q
Sbjct: 10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL-GTNAITADKVQGVKDRFMSAYDITADGRLQIQELANMILPEDENFLL--LFRRETPLDNSVEFMRIWRKFDEDSSGFISAVELRNFLQDLFLQHKKHITGDKLDEYTDTMMKLFNRNKDGRLDLNDLAKILALQ 176
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P07090|CALB2_CHICK (Calretinin OS=Gallus gallus OX=9031 GN=CALB2 PE=2 SV=3) HSP 1 Score: 214.542 bits (545), Expect = 2.289e-66 Identity = 120/273 (43.96%), Postives = 181/273 (66.30%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
L +A+QF +VW H+DADGNGYIEG EL++F E + G ++ +++L D ++F+ +D+N+D KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L + F+ K+++ + +F YD+D +G I+ EL LKDL +++ L +R+ I+N ++ GK+ EL+++L + L
Sbjct: 11 LAELSASQFLDVWRHFDADGNGYIEGKELENFFQELESARKGTGVDS---KRDSLGDKMKEFMHKYDKNADGKIEMAELAQILPTEENFLLCFRQH--VGSSSEFMEAWRRYDTDRSGYIEANELKGFLSDLLKKANRP---YDEAKLQEYTQTILRMFDMNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLSSEEFNAIFAFYDKDGSGFIDEHELDALLKDLYEKNKKEMSIQQLTNYRRSIMN---LSDGGKLYRKELEVVLCSEPPL 269
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q5XJX1|SEGN_DANRE (Secretagogin OS=Danio rerio OX=7955 GN=scgn PE=1 SV=1) HSP 1 Score: 214.542 bits (545), Expect = 2.404e-66 Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 3
Query: 153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
N A F ++W H+DAD NGYIEG ELD F + + + P D++ E + +K+ F+ A+D D ++++ ELA +LP +E FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELKNFL + + + ++I KL EYT M+++FD NKDG+L L+++AR+L ++EN+L + + ++ D E +F YD + G +E E+ GF+KD++ LV+ DL+ FR+ +L D+N DGKI +EL + L
Sbjct: 7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHML-----KKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHK--KKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCL 266 HSP 2 Score: 97.4413 bits (241), Expect = 6.472e-22 Identity = 56/175 (32.00%), Postives = 105/175 (60.00%), Query Frame = 3
Query: 435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
A LD++ F++IW+ FD D +G+IE EL +F LK+ +P+++I++E++ + + + +D+ DG+LQ+ E+A + LP +EN+L IF+ + +V+ ++ YD D +G I EL+ FLKDL ++ + L+ + ++ +D N DG++ +N+L +L Q
Sbjct: 6 ANLDAA-GFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQ 177 HSP 3 Score: 96.6709 bits (239), Expect = 1.438e-21 Identity = 58/176 (32.95%), Postives = 103/176 (58.52%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKE 683
+ +F ++W YDAD +GYI +EL +FL + + + I P++L++ T + +K FD+N D ++++++LA++L E FLL F+ DA ++ +F KI+ +D ++G +E E+ F+ + ++ RP IS L ++ +L D NKDGK+Q SE+A L +K
Sbjct: 103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ-HKKKIPPNKLDEYTDAMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRP--SISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267G6Z8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033745g2 PE=4 SV=1) HSP 1 Score: 422.55 bits (1085), Expect = 4.364e-145 Identity = 199/287 (69.34%), Postives = 247/287 (86.06%), Query Frame = 3
Query: 105 TNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
NFLAQ++D T++L+NFTATQFAEVWSHYD DGNG+IEG ELD FL EFI S++ EDI P+ +++ L+ +KRDF+DAFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IW+QFDKD SG+IEADEL++FL L A + +SE+KL+EYTSTMLQLFD N+DGKLQLSEMA+LLPVKENYLCRPIFKNA++I + DI+ VF+LYDRDKNG IENEELQGFLKDLL L QEDYD D+ FF+++IL++WDVNHDGKIS +ELKM+L+QQSRLA
Sbjct: 14 ANFLAQFRDQNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDIGPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCA--DRCVSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQEDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMILLQQSRLA 298
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A1I8GV54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003004g1 PE=4 SV=1) HSP 1 Score: 422.55 bits (1085), Expect = 4.509e-145 Identity = 199/287 (69.34%), Postives = 247/287 (86.06%), Query Frame = 3
Query: 105 TNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
NFLAQ++D T++L+NFTATQFAEVWSHYD DGNG+IEG ELD FL EFI S++ EDI P+ +++ L+ +KRDF+DAFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IW+QFDKD SG+IEADEL++FL L A + +SE+KL+EYTSTMLQLFD N+DGKLQLSEMA+LLPVKENYLCRPIFKNA++I + DI+ VF+LYDRDKNG IENEELQGFLKDLL L QEDYD D+ FF+++IL++WDVNHDGKIS +ELKM+L+QQSRLA
Sbjct: 14 ANFLAQFRDQNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDIGPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCA--DRCVSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQEDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMILLQQSRLA 298
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GD68 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g2 PE=4 SV=1) HSP 1 Score: 421.009 bits (1081), Expect = 1.863e-144 Identity = 204/300 (68.00%), Postives = 250/300 (83.33%), Query Frame = 3
Query: 87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE-------DYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
MS K NFL+Q++D TKKL+NFTATQFAEVWSHYD DGNG+IEG+ELD FL EFI S++ ED + +++ ++ +KRDF++AFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IWKQFDKD SGFIEADELK+FL L A ++ ++E+KLIEYT TMLQLFD N+DGKLQLSEMARLLPVKENYLCRPIFKNA KI++ DI+ VF LYDRDKNGTIENEELQGFLKDLL L QE DYD D++FF+++IL++WDVNHDGKIS +ELKM+LMQQSR+A
Sbjct: 1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKRDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSA--DKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGMTQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRIA 298
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GV22 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g1 PE=4 SV=1) HSP 1 Score: 419.468 bits (1077), Expect = 7.226e-144 Identity = 203/300 (67.67%), Postives = 250/300 (83.33%), Query Frame = 3
Query: 87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE-------DYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
MS K NFL+Q++D TKKL+NFTATQFAEVWSHYD DGNG+IEG+ELD FL EFI S++ ED + +++ ++ +K+DF++AFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IWKQFDKD SGFIEADELK+FL L A ++ ++E+KLIEYT TMLQLFD N+DGKLQLSEMARLLPVKENYLCRPIFKNA KI++ DI+ VF LYDRDKNGTIENEELQGFLKDLL L QE DYD D++FF+++IL++WDVNHDGKIS +ELKM+LMQQSR+A
Sbjct: 1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSA--DKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGMTQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRIA 298
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267FNU4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g3 PE=4 SV=1) HSP 1 Score: 419.468 bits (1077), Expect = 7.801e-144 Identity = 203/300 (67.67%), Postives = 250/300 (83.33%), Query Frame = 3
Query: 87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE-------DYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
MS K NFL+Q++D TKKL+NFTATQFAEVWSHYD DGNG+IEG+ELD FL EFI S++ ED + +++ ++ +K+DF++AFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IWKQFDKD SGFIEADELK+FL L A ++ ++E+KLIEYT TMLQLFD N+DGKLQLSEMARLLPVKENYLCRPIFKNA KI++ DI+ VF LYDRDKNGTIENEELQGFLKDLL L QE DYD D++FF+++IL++WDVNHDGKIS +ELKM+LMQQSR+A
Sbjct: 1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSA--DKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGITQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRIA 298
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:103030695]) HSP 1 Score: 220.705 bits (561), Expect = 1.035e-69 Identity = 118/271 (43.54%), Postives = 176/271 (64.94%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFK-NADKI--TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
L N A F ++W H+DAD NGYIEG ELD+F + + P D + +E + LK F+ A+D +D ++++ ELA +LP DE FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELK+FL + + + E +S KL EYT TM+ +FD NKDG+L L+++AR+L ++EN+L + FK +A+ + D E +F YD K G +E E+ GF+KD++ LV+ +L+ FR+++L D+N DGKI NEL + L
Sbjct: 5 LDNLDAAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKL-----RPGDNVTEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLLVFRREAPLDNSVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKKE--LSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQ--FKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRPSISGSELDQFRKVLLRHCDINKDGKIQKNELALCL 266 HSP 2 Score: 97.8265 bits (242), Expect = 5.277e-23 Identity = 56/178 (31.46%), Postives = 100/178 (56.18%), Query Frame = 3
Query: 432 DAPLDS--SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
D LD+ + F++IW+ FD D +G+IE EL F + + RP + ++EEK+ + + + +D DG+LQ+ E+A + LP EN+L +F+ + +V+ ++ YD D +G I EL+ FL+DL +++ LE + ++N +D N DG++ +N+L +L Q
Sbjct: 2 DCDLDNLDAAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKLRPGDNVTEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLL--VFRREAPLDNSVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQ 177 HSP 3 Score: 97.8265 bits (242), Expect = 6.130e-23 Identity = 57/175 (32.57%), Postives = 99/175 (56.57%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+ +F ++W YDAD +GYI EL SFL + + EL+ L + ++ FD+N D ++++++LA++L E FLL F+ DA L+ +F KI+ +D ++G +E E+ F+ + ++ RP IS +L ++ +L+ D NKDGK+Q +E+A L +K
Sbjct: 103 SVEFMKIWRKYDADSSGYISAVELKSFLQDLFQH------HKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQFKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRP--SISGSELDQFRKVLLRHCDINKDGKIQKNELALCLGMK 269
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2a (calretinin [Source:NCBI gene;Acc:103033342]) HSP 1 Score: 218.394 bits (555), Expect = 8.490e-69 Identity = 120/272 (44.12%), Postives = 179/272 (65.81%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
L TA+QF ++W H+D DGNGYIEG EL++F E V+ G ++P + + + +DF+ FD+N D +IEMSELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SG+IEA+ELK FLS+ LK+A ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F+ K+T +F YD+D NG I+ +EL L+DL +++ D DL +++ I+ ++ GK+ +EL+++L ++ L
Sbjct: 13 LAELTASQFLDIWKHFDTDGNGYIEGKELENFFRELEVARRGAGVDPTNV---SFREKMKDFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSSEFMAAWRRYDTDRSGYIEANELKGFLSDLLKKA--NRHYDDKKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGI-KLTAEQFNAIFTYYDKDGNGYIDEQELDALLRDLYEKNKKEVDTKDLTGYKKSIMA---LSDGGKLYRSELEIVLCREPML 271
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108]) HSP 1 Score: 198.749 bits (504), Expect = 3.450e-61 Identity = 124/269 (46.10%), Postives = 176/269 (65.43%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
L TA+QF +VW H+D+DGNG+IEG EL+ FL E + G +N + +N +L DF+ FDQNSD KIEM ELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SGFIEA+ELK FLS+ L +A Q ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F++ K+++ + +F YD+D NG I+ EL LKDL D+D L ++ I+ ++ GK+ EL+++L ++
Sbjct: 10 LAELTASQFMDVWRHFDSDGNGFIEGQELEDFLEELESARKG--VNAEPVNA-SLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKAN--RQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQSF-KLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKHNMDFDSQTLASSKKSIMA---LSDGGKLFRTELEIVLCKE 265
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108]) HSP 1 Score: 197.208 bits (500), Expect = 1.280e-60 Identity = 124/269 (46.10%), Postives = 174/269 (64.68%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
L TA+QF +VW H+D+DGNG+IEG EL+ FL E + G +NP +L DF+ FDQNSD KIEM ELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SGFIEA+ELK FLS+ L +A Q ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F++ K+++ + +F YD+D NG I+ EL LKDL D+D L ++ I+ ++ GK+ EL+++L ++
Sbjct: 16 LAELTASQFMDVWRHFDSDGNGFIEGQELEDFLEELESARKG--VNP---VNASLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKA--NRQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQSF-KLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKHNMDFDSQTLASSKKSIMA---LSDGGKLFRTELEIVLCKE 269
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb1 (calbindin 1 [Source:NCBI gene;Acc:103033937]) HSP 1 Score: 188.348 bits (477), Expect = 2.661e-57 Identity = 109/268 (40.67%), Postives = 168/268 (62.69%), Query Frame = 3
Query: 159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKET---LSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
+A+QF +++ HYD DGNGYIEG EL +F+ E + K+ L+D R F++ +++N+D KI++ EL Q+LPT+E FLL FR+ L S EFM+ W+ +D D SG+IE+DELKNFL + L+ R ++ E+KL EYT T L++FDSN DGKL LSEMARLLP EN+L + F+ K+ + +F LYD+D+NG ++ EL LKDL ++ + ++ +++ I+ ++ GK+ EL ++L ++
Sbjct: 12 SASQFLDIFHHYDNDGNGYIEGKELQNFIKEL-----------QQARKQAGLELTDQMRAFVEEYEKNADGKIDIVELVQILPTEENFLLFFRQ--QLKSCTEFMEAWRTYDADHSGYIESDELKNFLRDLLQ--RAKKPFDEKKLEEYTLTTLKIFDSNNDGKLCLSEMARLLPDDENFLLK--FEGV-KMARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNKKVLEIGKIQVYKKAIMA---LSDGGKLYRTELALVLCEE 258
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010]) HSP 1 Score: 204.527 bits (519), Expect = 4.475e-64 Identity = 111/258 (43.02%), Postives = 162/258 (62.79%), Query Frame = 3
Query: 177 EVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADK-ITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
EVW +DAD NGYIEG ELD F + + ++ DE + ++R+ + +D+++D ++ +SE+A +L DE FLL+F++ + LD+SVEF+KIW Q+D D SGFI A ELKNFL + L + Q+ E+KL EYT TM++LFD NKDG+L L++MAR+L +KENYL + ++A + D E +F YD + G +E E+ GF+KD++ LV+ DL FR +L D N DGKI EL + L
Sbjct: 16 EVWQKFDADDNGYIEGKELDEFFKHLLKQLAAKECVSDE----KVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLLIFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLL--SHHGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVADLSKFRDSLLRRCDTNQDGKIQKWELALCL 267 HSP 2 Score: 95.9005 bits (237), Expect = 5.545e-23 Identity = 54/164 (32.93%), Postives = 97/164 (59.15%), Query Frame = 3
Query: 459 FMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK-ENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
+++W++FD D +G+IE EL F LK+ +E +S+EK+ ++ ++D + DG+L LSE+A +L + E++L IFK A + +V+ ++H YD D +G I EL+ FLKDLL+ ++ LE + ++ +D N DG++ +N++ +L
Sbjct: 14 LLEVWQKFDADDNGYIEGKELDEFFKHLLKQLAAKECVSDEKVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLL--IFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLLSHHGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARIL 175 HSP 3 Score: 83.1889 bits (204), Expect = 1.805e-18 Identity = 50/178 (28.09%), Postives = 104/178 (58.43%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFR-KDAPL-DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENY 689
+ +F ++W YD D +G+I SEL +FL + ++S G + +L + T + +K FD+N D +++++++A++L E +LL F +DA + +F KI+ +D ++G +E E+ F+ + ++ RP +++ L ++ ++L+ D+N+DGK+Q E+A L ++ +
Sbjct: 104 SVEFIKIWHQYDLDSSGFISASELKNFLKD-LLSHHGRQVEEKKLEEYTDTMMK-----LFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVAD--LSKFRDSLLRRCDTNQDGKIQKWELALCLGIRTAH 273
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 101.293 bits (251), Expect = 2.704e-26 Identity = 62/146 (42.47%), Postives = 96/146 (65.75%), Query Frame = 3
Query: 519 LKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQS 956
L++FL + LK+A E E+KL EYT T L++FDSN+DGKL+LSEMARLLPV+EN+L + F+ +T + ++F YD+D+NG I+ +EL+ L+DL ++D D L +++ I+ D GK+ N+L+M+L +S
Sbjct: 1 LQDFLKDLLKKANRE--FDEKKLNEYTQTTLKMFDSNRDGKLELSEMARLLPVQENFLLK--FQGV-TMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNRKDLDAQTLTSYKRNIMALSD---HGKLYRNDLEMVLCVES 138 HSP 2 Score: 59.6918 bits (143), Expect = 2.452e-11 Identity = 41/129 (31.78%), Postives = 74/129 (57.36%), Query Frame = 3
Query: 291 ELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPV 677
E +++ L++ + L FD N D K+E+SE+A++LP E FLL F+ ++ EF I+ +DKD++G+I+ DEL+ L + ++ R + + + L Y ++ L D GKL +++ +L V
Sbjct: 15 EFDEKKLNEYTQTTLKMFDSNRDGKLELSEMARLLPVQENFLLKFQ--GVTMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNRKD--LDAQTLTSYKRNIMALSDH---GKLYRNDLEMVLCV 136
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000059.1 (pep scaffold:Pmarinus_7.0:GL485405:3181:11335:1 gene:ENSPMAG00000000055.1 transcript:ENSPMAT00000000059.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 85.5001 bits (210), Expect = 3.118e-21 Identity = 39/78 (50.00%), Postives = 60/78 (76.92%), Query Frame = 3
Query: 291 ELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELK 524
EL + + + + F++ +D+N+D KIEM+ELAQ+LPT+E FLL FR+ + SS EFM+ W+++D D+SG+IEA+ELK
Sbjct: 2 ELPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFLLFFRQH--VKSSAEFMETWRRYDTDRSGYIEANELK 77 HSP 2 Score: 47.3654 bits (111), Expect = 1.261e-7 Identity = 24/80 (30.00%), Postives = 49/80 (61.25%), Query Frame = 3
Query: 564 EQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQ 803
+++ E + E + ++ +D N DGK++++E+A++LP +EN+L F+ K ++ + + YD D++G IE EL+
Sbjct: 1 QELPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFLL--FFRQHVK-SSAEFMETWRRYDTDRSGYIEANELK 77
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001806.1 (pep scaffold:Pmarinus_7.0:GL476330:305753:307416:1 gene:ENSPMAG00000001642.1 transcript:ENSPMAT00000001806.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.151 bits (139), Expect = 1.200e-11 Identity = 27/48 (56.25%), Postives = 39/48 (81.25%), Query Frame = 3
Query: 324 RDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMK 467
++F++ FDQNSD +IE++ELAQ+LPT+E FLL FR+ + SS EFM+
Sbjct: 7 KEFMEKFDQNSDGRIELAELAQILPTEENFLLFFRQH--VKSSAEFME 52
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452]) HSP 1 Score: 48.521 bits (114), Expect = 2.100e-7 Identity = 42/172 (24.42%), Postives = 79/172 (45.93%), Query Frame = 3
Query: 165 TQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+F E +S +D DG+G I EL + ++ LG++ EL +D ++ D + + I+ E ++ RK DS E + ++ FDKD +G+I A EL++ ++ L E +E++ E M++ D + DG++ E +++ K
Sbjct: 11 AEFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVQMMTAK 149
BLAST of Calbindin-32 vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313]) HSP 1 Score: 43.8986 bits (102), Expect = 5.958e-6 Identity = 36/124 (29.03%), Postives = 59/124 (47.58%), Query Frame = 3
Query: 165 TQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLS 536
+F E ++ +D D NG I SEL + ++ LG + E+N D ++ D + +++IE SE L+ R+ DS E ++ +K FDK+ G I A ELK+ L+
Sbjct: 11 AEFKEAFALFDKDNNGSISSSELAT-----VMRSLGLSPSEAEVN---------DLMNEIDVDGNHQIEFSEF---------LALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLT 111
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45967 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ7]) HSP 1 Score: 159.073 bits (401), Expect = 2.083e-46 Identity = 96/269 (35.69%), Postives = 154/269 (57.25%), Query Frame = 3
Query: 144 KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQED--YDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
K + +F +V+ +D+D NGYIE E+D F+ + + +++N E K ++D K++ L+ +D + D + M EL +LP + L + + SV+FMKI+ +FD+D+SGF+E EL+ F E L + + I+ KL +Y L+ +D+NKDGK L+E+A++LP++EN + +T D V+ YD+D +G IE +E+ L D+L Q D Y+K DLE FR IIL +D N D K+S +EL +L
Sbjct: 4 KATKLSPVEFVKVFMKFDSDKNGYIEEKEMDLFIHDLL-----KELNDKEPTKAEVADKKKEILEKYDLDKDGRFSMDELENILPVEPNLLRTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLS-MKGKSSINPRKLDDYIEYCLEKYDANKDGKFNLNELAKMLPLEENIF--ETYGIKRDMTREDFNKVWDYYDKDNSGEIEGDEILALLHDVLKQQQPDREYNKPDLERFRDIILEIYDENKDNKLSRDELCCLL 264
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45965 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ5]) HSP 1 Score: 126.716 bits (317), Expect = 4.981e-35 Identity = 81/178 (45.51%), Postives = 117/178 (65.73%), Query Frame = 3
Query: 156 FTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENY 689
T+ QF +++ YDADGN I+ ELD FL +D+ + N L LK + L+ +D N D I+MSELAQ LP +E FL+ FRKD + SSV+FMKI+K++D D+SG+IE ELK FL + L + + IS ++ +Y S M+++FD NKDGKL+LSE++RLL V++N+
Sbjct: 22 LTSEQFYDIFKKYDADGNDCIDAKELDLFL---------QDVQKADPNATNLDALKNEILEKYDANYDGVIQMSELAQCLPLEENFLVGFRKDNVI-SSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLDADK--KAISPSRINDYASAMIEIFDRNKDGKLELSELSRLLQVEDNF 187 HSP 2 Score: 79.337 bits (194), Expect = 1.775e-17 Identity = 53/156 (33.97%), Postives = 91/156 (58.33%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGK 914
+S +F I+K++D D + I+A EL FL + +++A P + L + +L+ +D+N DG +Q+SE+A+ LP++EN+L F+ + I++VD +F YD DK+G IE EL+ FL DLL ++ + + ++ +D N DGK
Sbjct: 23 TSEQFYDIFKKYDADGNDCIDAKELDLFLQD-VQKADP----NATNLDALKNEILEKYDANYDGVIQMSELAQCLPLEENFLVG--FRKDNVISSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLDADKKAISPSRINDYASAMIEIFDRNKDGK 171
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45966 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ6]) HSP 1 Score: 128.642 bits (322), Expect = 6.328e-35 Identity = 86/258 (33.33%), Postives = 140/258 (54.26%), Query Frame = 3
Query: 177 EVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSE-FLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDL-EFFRQIILNEWDVNHDGKISMNELKMML 944
E++S +D DGNG+I+ ELDSF+ +DI +EL+++ ++ K L D+N+DNKI EL +VLPT F+ F V+F+KIWK +DKD SGF+E EL+ FL E F P ++I E++ ++ + +L DGK + + + + + C + NA D + F YD+D +G IE+ EL+ +DLL + D ++D+ ++ + +++ + D N D KI +ELK L
Sbjct: 23 EIFSKFDKDGNGFIDAKELDSFI---------KDICNNELSEDDVASFKTGLLAKLDENNDNKISKKELRKVLPTKSNFMRQFLTTKEF-CQVDFIKIWKNYDKDGSGFLEKPELQLFLEELFAVNKTPGQRIDEKEREKFQTILL-------DGKEGVDKKSLMKHLGVKAACDEVAANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLKINDADITEEDVKDYVQSVVMPDLDKNKDNKIQKDELKSYL 263 HSP 2 Score: 61.6178 bits (148), Expect = 6.824e-11 Identity = 55/193 (28.50%), Postives = 87/193 (45.08%), Query Frame = 3
Query: 105 TNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYT-STMLQLFDSNKDGKLQLSEMARLLPVK 680
+NF+ Q+ + F F ++W +YD DG+G++E EL FL E DE +E + D + D+ S M L DEV +A +F + + ++DKD SG IE+ EL+ + LK + I+EE + +Y S ++ D NKD K+Q E+ L +K
Sbjct: 90 SNFMRQFL-----TTKEFCQVDFIKIWKNYDKDGSGFLEKPELQLFLEELFAVNKTPGQRIDEKEREKFQTILLDGKEGVDKKS----LMKHLGVKAACDEV-----AANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLK--INDADITEEDVKDYVQSVVMPDLDKNKDNKIQKDELKSYLKIK 266
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO46016 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ4]) HSP 1 Score: 76.2554 bits (186), Expect = 2.071e-15 Identity = 48/172 (27.91%), Postives = 90/172 (52.33%), Query Frame = 3
Query: 456 EFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ-EDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAK 968
+ IW ++D ++ G + ++LK F+++ +K +++S + + L D ++ G++ L EMAR L V++N+L K+ D ++ YD+D G+I+ EL ++D+ A +Q + DD+E ++ II+ D + DG IS EL + Q + AK
Sbjct: 166 RYGTIWNRYDVNREGSLSTEQLKGFINDSMKG----QKLSSKNIGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNINPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDVFAKIQGRSPEMDDIEKWKDIIMMFADADGDGAISKEELNIFFAQGLKEAK 333 HSP 2 Score: 48.521 bits (114), Expect = 2.568e-6 Identity = 38/166 (22.89%), Postives = 83/166 (50.00%), Query Frame = 3
Query: 168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLD-SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLF-DSNKDGKLQLSEM 659
++ +W+ YD + G + +L F+++ S+ G+ +L+ + + L + LDA D++ ++++ E+A+ L ++ FL F + +F +IW +D+D G I+ EL + + A+ + + E IE ++ +F D++ DG + E+
Sbjct: 166 RYGTIWNRYDVNREGSLSTEQLKGFIND---SMKGQ-----KLSSKNIGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNINPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDVF--AKIQGRSPEMDDIEKWKDIIMMFADADGDGAISKEEL 321
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45935 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRE8]) HSP 1 Score: 55.8398 bits (133), Expect = 1.244e-9 Identity = 46/176 (26.14%), Postives = 82/176 (46.59%), Query Frame = 3
Query: 156 FTATQFAEV---WSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIW-KQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLL 671
T Q +E+ + YD +G+G I EL + E G+ ++ +EL +D + A D + + K+E E Q++ + AP VE MK + +FD+D +GFI++DE+K + F ++ + L + M++ D+N DGK+ E ++L
Sbjct: 5 LTPEQLSEIEAAFKMYDTNGDGQISAEELGQAMRE-----AGQLVSDEEL---------KDMIRAVDLDGNGKVEFKEFVQMMANQ------LGQPAP----VEEMKAYFDRFDQDGNGFIDSDEMKCLVRAFYS------NLTGDALKQQVRAMIEAADTNSDGKISFEEFVKVL 150
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000016338.2 (calretinin [Source:NCBI gene;Acc:101174993]) HSP 1 Score: 218.009 bits (554), Expect = 1.153e-68 Identity = 118/269 (43.87%), Postives = 178/269 (66.17%), Query Frame = 3
Query: 147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
L TA+QF ++W H+D DGNGYIEG EL++F E S G ++P + + R+F+ FD+N D +IEMSELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SG+IEA+ELK FLS+ L++A E+KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F+ K+T+ ++F YD+D NG I+ +EL+ L+DL +++ D L +++ I++ ++ GK+ EL+++L ++
Sbjct: 13 LAEVTASQFLDIWKHFDVDGNGYIEGKELENFFRELETSRRGAGVDP---SNPQFREKMREFMQKFDKNRDGRIEMSELAQILPTEENFLLCFRQ--FVSSSAEFMAAWRRYDTDRSGYIEANELKGFLSDLLEKA--NRHYDEQKLHEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGV-KLTSEQFNSIFTYYDKDGNGYIDEQELEALLRDLYQTNKKEIDAKSLMSYKESIMS---LSDGGKLYRGELEIVLCKE 268
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606]) HSP 1 Score: 213.001 bits (541), Expect = 1.000e-66 Identity = 117/273 (42.86%), Postives = 180/273 (65.93%), Query Frame = 3
Query: 144 KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
+L TA QF ++W H+D DGNGYIEG EL++F ++ + G ++P + + + R+F+ FD+NSD +IEMSELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SG+IE++ELK FLS+ LK+A +++L EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F+ ++T + +++F YD+D NG I+ +EL LKDL + D D L +++ I+ ++ GK+ E++++L + S L
Sbjct: 12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP---SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKA--NRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGI-RLTVKEFDSLFTFYDKDGNGYIDEQELDALLKDLCDKNKMDVDATGLVDYKKSIMA---LSDGGKLYRTEMEIVLCRDSML 271
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000006100.2 (secretagogin [Source:NCBI gene;Acc:101157482]) HSP 1 Score: 203.371 bits (516), Expect = 5.216e-63 Identity = 111/271 (40.96%), Postives = 171/271 (63.10%), Query Frame = 3
Query: 153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSD-LKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQS 956
N A F E+W H+DAD NGYIEG ELD F + + + P E E LK F+ A+D +D ++++ ELA +LP +E FLL+F ++APLD+SV+FMKIW+++D D SG+I A ELK FL + ++ E +S +KL EYT M+++FD NKDG+L L+++AR+L +++N+L + + ++ + D E +F YD K G +E E+ GF+KD++ LV+ +L+ R ++L+ DVN+DGKI NEL + L +S
Sbjct: 7 NLDAAGFLEIWQHFDADDNGYIEGKELDEFFHHMM-----QRLGPQEETTEERVRELKERFMSAYDVTADGRLQIQELASMILPEEENFLLIFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHHRE--VSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMDASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELVRPSLSGPELDKLRAVLLHHCDVNNDGKIQKNELALCLGVKS 270
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606]) HSP 1 Score: 201.83 bits (512), Expect = 1.677e-62 Identity = 115/273 (42.12%), Postives = 174/273 (63.74%), Query Frame = 3
Query: 144 KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
+L TA QF ++W H+D DGNGYIEG EL++F ++ + G ++P + + + R+F+ FD+NSD +IEMSELAQ+LPT+E FLL FR+ + SS EFM W+++D D+SG+IE++ELK FLS+ LK+A +++L EYT T+L++FD N DGKL LSEMARLLPV+EN+L + F+ I+ + L +D NG I+ +EL LKDL + D D L +++ I+ ++ GK+ E++++L + S L
Sbjct: 12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP---SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKA--NRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQKRPPISLTCV-----LVYQDGNGYIDEQELDALLKDLCDKNKMDVDATGLVDYKKSIMA---LSDGGKLYRTEMEIVLCRDSML 267 HSP 2 Score: 112.849 bits (281), Expect = 1.343e-28 Identity = 68/188 (36.17%), Postives = 110/188 (58.51%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEAR------PEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYL-CRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML-MQQSRLAKLTHLEP 986
++ +F+ IWK FD D +G+IE EL+NF ++ L+ AR P +EK+ E+ + FD N DG++++SE+A++LP +EN+L C F ++ + N + YD D++G IE+ EL+GFL DLL YD L + Q IL +D+N DGK+ ++E+ +L +Q++ L K P
Sbjct: 17 TAAQFIDIWKHFDTDGNGYIEGKELENFFTQ-LESARRGAGVDPSHASFKEKMREFMAN----FDKNSDGRIEMSELAQILPTEENFLLCFREFVG----SSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKANRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQKRPP 195
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: SCGN (secretagogin [Source:NCBI gene;Acc:101170527]) HSP 1 Score: 178.718 bits (452), Expect = 1.733e-53 Identity = 103/264 (39.02%), Postives = 158/264 (59.85%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQV-LPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLL--PVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
A F ++W ++D D NGYIE ELD+F ++ + +D+ D++ +L+R A ++D ++ + +LA V LP +E FLLLFR++ PLD+SVEFMKIW+++D+D SG+I A+ELK FL + + R + I+ KL EYT TML++FD N+ GKL L+++ARLL V C +N D E +F YD K G +E E+ G + D++ L + +L FR+ +++ D+N DGKI NEL + L
Sbjct: 10 AAGFLQIWLYFDKDDNGYIEQKELDAFFKHMLLHLGMKDVTEDKIQ-----ELRRRC--AHRPSADGRLLIQQLASVMLPEEENFLLLFRQETPLDNSVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHR--KSITASKLEEYTVTMLKIFDKNQGGKLDLNDLARLLLGYVVTFQACSREDRNR------DFEKIFAHYDVSKTGALEGPEVDGLVMDMMELAKPSISGAELNKFRKALMSHCDINRDGKIQKNELALCL 258 HSP 2 Score: 77.411 bits (189), Expect = 6.841e-16 Identity = 48/173 (27.75%), Postives = 97/173 (56.07%), Query Frame = 3
Query: 162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
+ +F ++W YD D +GYI +EL FL + + + I +L + T++ LK FD+N K+++++LA++L +++ F+ + D + +F KI+ +D ++G +E E+ + + ++ A+P IS +L ++ ++ D N+DGK+Q +E+A L +K
Sbjct: 100 SVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHR-KSITASKLEEYTVTMLK-----IFDKNQGGKLDLNDLARLLLG---YVVTFQACSREDRNRDFEKIFAHYDVSKTGALEGPEVDGLVMDMMELAKP--SISGAELNKFRKALMSHCDINRDGKIQKNELALCLGLK 261 HSP 3 Score: 72.7886 bits (177), Expect = 3.205e-14 Identity = 53/176 (30.11%), Postives = 92/176 (52.27%), Query Frame = 3
Query: 432 DAPLDS--SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMAR-LLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLM 947
D LD + F++IW FDKD +G+IE EL F L ++ ++E+K+ E + DG+L + ++A +LP +EN+L +F+ + +V+ ++ YD D +G I EL+GFL+DL ++ LE + +L +D N GK+ +N+L +L+
Sbjct: 2 DGALDKLDAAGFLQIWLYFDKDDNGYIEQKELDAFFKHMLLHLGMKD-VTEDKIQELRRRCAH--RPSADGRLLIQQLASVMLPEEENFLL--LFRQETPLDNSVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHRKSITASKLEEYTVTMLKIFDKNQGGKLDLNDLARLLL 172
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000055605.1 (SMESG000055605.1) HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0 Identity = 301/302 (99.67%), Postives = 301/302 (99.67%), Query Frame = 3
Query: 87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEPRQ 992
MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQI EEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEPRQ
Sbjct: 1 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQILEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEPRQ 302
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000023194.1 (SMESG000023194.1) HSP 1 Score: 370.933 bits (951), Expect = 3.767e-128 Identity = 177/287 (61.67%), Postives = 231/287 (80.49%), Query Frame = 3
Query: 108 NFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAK 968
NFL++++D++T++ NFTA QF EVW HYD DGNGYIEG ELD FL EFI S++ I + ++ LSD+K DF+ A+D+N DNKIE+ ELAQ+LPTDE FLLLFR+D PL+SSV+FMKIW++FD D SG+IE+DELK FL + E+ +E IS+EKL EY+ T+LQLFD N DGKLQLSEMARLLPVKENYL +P+FKN ++IT DI+ VF YD DKNGTIENEELQG LKDLL L E YD++ ++F++++IL +WD++HDGKI +ELKM+L+QQS++A+
Sbjct: 10 NFLSKFRDSKTRRWTNFTAKQFLEVWCHYDTDGNGYIEGEELDGFLREFISSVVPGSIGSEIISDSALSDMKEDFMAAYDENEDNKIEIGELAQMLPTDENFLLLFRRDNPLESSVDFMKIWRKFDTDNSGYIESDELKEFLRYLISESNVKE-ISDEKLEEYSYTILQLFDENGDGKLQLSEMARLLPVKENYLSKPLFKNVNRITGYDIDRVFKKYDTDKNGTIENEELQGLLKDLLELAGESYDEEKMKFYQEVILGQWDLDHDGKIGRDELKMILLQQSKMAQ 295
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000003798.1 (SMESG000003798.1) HSP 1 Score: 320.472 bits (820), Expect = 4.867e-108 Identity = 154/297 (51.85%), Postives = 222/297 (74.75%), Query Frame = 3
Query: 93 TTKFT-NFLAQYKDNETK-KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLK-EARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLT 974
+ KFT N+L +Y++ E +L FTATQF +VW+H+D D NGYIE +ELD+FL +F S++ E I + L++ +K+ FL+A+D+N D +IE+ EL+Q+LPTDE FL+LF+++ P+ SSVEFMKIWK+FDKD+SGFIEA+EL+ F++E LK + E++ E K++ +T+ ML+LFD N DGKLQLSEMARLLP+KENYL +P+ N KI+ +++E +F YD D+NG ++ E+Q L DL+ L +E YD LE F+ +IL++WDVN DGKISM ELKM+L Q S+L +++
Sbjct: 20 SQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKSAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEPYDDKKLEKFQNVILSQWDVNKDGKISMKELKMILTQHSKLGEIS 316
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1) HSP 1 Score: 237.654 bits (605), Expect = 1.894e-77 Identity = 117/198 (59.09%), Postives = 154/198 (77.78%), Query Frame = 3
Query: 87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDE----LNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL 668
MS K NFL+Q++D TKKL+NFTAT+FAEVW+HYD DGNGYIEG ELD FL EF+ S++ E+ + + L++ + LK + ++A+D+N+D +IE+ ELAQ+LP +E FLLLFR+D L+SSV+F+K+W+ FDK+QSGFIEA+ELK FL LKEA+P E +EK+ EYT +LQLFD NKDGKLQLSEMARL
Sbjct: 1 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 198 HSP 2 Score: 103.219 bits (256), Expect = 7.702e-26 Identity = 60/176 (34.09%), Postives = 113/176 (64.20%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPE--EQISEEKLIEYTST------MLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLL--ALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMM 941
++ EF ++W +DKD +G+IE DEL FL EF+ E + S+EK++ T+ +++ +D N DG++++ E+A++LP++EN+L +F+ +++ ++VD V+ ++D++++G IE EL+ FLK LL A ED K+ ++ + +IIL +D N DGK+ ++E+ +
Sbjct: 25 TATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 198
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1) HSP 1 Score: 234.958 bits (598), Expect = 2.927e-76 Identity = 120/209 (57.42%), Postives = 155/209 (74.16%), Query Frame = 3
Query: 87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPD--ELNKE-------------TLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL 668
MS K NFL+Q++D TKKL+NFTAT+FAEVW+HYD DGNGYIEG ELD FL EF+ S++ E+ + + ELNK + LK + ++A+D+N+D +IE+ ELAQ+LP +E FLLLFR+D L+SSV+F+K+W+ FDK+QSGFIEA+ELK FL LKEA+P E +EK+ EYT +LQLFD NKDGKLQLSEMARL
Sbjct: 1 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 209 HSP 2 Score: 98.5969 bits (244), Expect = 5.123e-24 Identity = 59/187 (31.55%), Postives = 111/187 (59.36%), Query Frame = 3
Query: 447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-------------RPEEQISEEKLIEYTST------MLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLL--ALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMM 941
++ EF ++W +DKD +G+IE DEL FL EF+ + E Q K++ T+ +++ +D N DG++++ E+A++LP++EN+L +F+ +++ ++VD V+ ++D++++G IE EL+ FLK LL A ED K+ ++ + +IIL +D N DGK+ ++E+ +
Sbjct: 25 TATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 209 The following BLAST results are available for this feature:
BLAST of Calbindin-32 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of Calbindin-32 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calbindin-32 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calbindin-32 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Calbindin-32 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calbindin-32 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30018274 ID=SMED30018274|Name=Calbindin-32|organism=Schmidtea mediterranea sexual|type=transcript|length=1131bpback to top protein sequence of SMED30018274-orf-1 >SMED30018274-orf-1 ID=SMED30018274-orf-1|Name=SMED30018274-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=303bp MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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