Calbindin-32

Overview
NameCalbindin-32
Smed IDSMED30018274
Length (bp)1131
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calbindin-32 (SMED30018274) t-SNE clustered cells

Violin plots show distribution of expression levels for Calbindin-32 (SMED30018274) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calbindin-32 (SMED30018274) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calbindin-32 (SMED30018274) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30018274

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018274SMESG000055605.1 Contig48130newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018274SMESG000055605.1 Contig48130uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018274SMESG000055605.1 Contig27065newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018274SMESG000055605.1 Contig27065uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neuronSMED30018274SMESG000055605.1 dd_Smed_v6_1230_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30018274SMESG000055605.1 dd_Smed_v6_1230_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30018274SMESG000055605.1 Contig4898GPL15192PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30018274SMESG000055605.1 dd_Smed_v4_1230_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calbindin-32 vs. Ensembl Human
Match: SCGN (secretagogin, EF-hand calcium binding protein [Source:HGNC Symbol;Acc:HGNC:16941])

HSP 1 Score: 210.305 bits (534), Expect = 2.179e-65
Identity = 123/271 (45.39%), Postives = 169/271 (62.36%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQV-LPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITT----VDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            L    A  F +VW  +DAD  GYIE  ELD+F    ++  LG D   D + K  L  +K+ F+   D + D +I M ELA + L  DE FLLLFR++ PLDSSVEFM+IW+++D D SGFI A EL+NFL +     +  + ISE KL EYT TM+++FD NKDG+L L+++AR+L ++EN+L +  FK  D  +T     D E +F  YD  K G +E  E+ GF+KD++ LVQ      DL+ FR+I+L   DVN DGKI  +EL + L
Sbjct:    9 LGRLDAAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMK-LGTD---DTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLLLFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHK--KAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQ--FK-MDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 270          

HSP 2 Score: 89.7373 bits (221), Expect = 8.654e-20
Identity = 53/178 (29.78%), Postives = 100/178 (56.18%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            ++ +F ++W  YDAD +G+I  +EL +FL +  +       +   +++  L +     +  FD+N D ++++++LA++L   E FLL F+ DA    +   +F KI+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L+  D NKDGK+Q SE+A  L +K N
Sbjct:  106 SSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLH------HKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQP--SISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 275          

HSP 3 Score: 75.485 bits (184), Expect = 8.442e-15
Identity = 46/170 (27.06%), Postives = 93/170 (54.71%), Query Frame = 3
Query:  450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            +  F ++W++FD D+ G+IE  EL  F    L +   ++ + +  L +     +   D++KDG++++ E+A + L   EN+L   +F+  + + ++V+   ++  YD D +G I   EL+ FL+DL    ++   +  LE +   ++  +D N DG++ +N+L  +L  Q
Sbjct:   14 AAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMKLGTDDTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLL--LFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQ 181          
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 209.92 bits (533), Expect = 3.347e-65
Identity = 118/273 (43.22%), Postives = 178/273 (65.20%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            L   TA+QF E+W H+DADGNGYIEG EL++F  E   +  G  +     N     +  ++F+  +D+NSD KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+T+ +   +F  YD+D++G I+  EL   LKDL    +++ +   L  +R+ +++   +   GK+   +L+++L  +  +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMS---LAEAGKLYRKDLEIVLCSEPPM 271          
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 209.92 bits (533), Expect = 3.347e-65
Identity = 118/273 (43.22%), Postives = 178/273 (65.20%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            L   TA+QF E+W H+DADGNGYIEG EL++F  E   +  G  +     N     +  ++F+  +D+NSD KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+T+ +   +F  YD+D++G I+  EL   LKDL    +++ +   L  +R+ +++   +   GK+   +L+++L  +  +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMS---LAEAGKLYRKDLEIVLCSEPPM 271          
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 193.741 bits (491), Expect = 1.948e-59
Identity = 107/231 (46.32%), Postives = 155/231 (67.10%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ 836
            L   TA+QF E+W H+DADGNGYIEG EL++F  E   +  G  +     N     +  ++F+  +D+NSD KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+T+ +   +F  YD++ N     ++L  + K +++L +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKEMN----IQQLTNYRKSVMSLAE 228          

HSP 2 Score: 110.923 bits (276), Expect = 7.565e-28
Identity = 62/174 (35.63%), Postives = 104/174 (59.77%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE-----EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            ++ +F++IWK FD D +G+IE  EL+NF  E  K  +    +S+     EK+ E+    +Q +D N DGK++++E+A++LP +EN+L    F+     ++ +    +  YD D++G IE  EL+GFL DLL      YD+  L+ + Q IL  +D+N DGK+ ++E+  +L  Q
Sbjct:   17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEF----MQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183          
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 193.741 bits (491), Expect = 1.948e-59
Identity = 107/231 (46.32%), Postives = 155/231 (67.10%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ 836
            L   TA+QF E+W H+DADGNGYIEG EL++F  E   +  G  +     N     +  ++F+  +D+NSD KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+T+ +   +F  YD++ N     ++L  + K +++L +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKEMN----IQQLTNYRKSVMSLAE 228          

HSP 2 Score: 110.923 bits (276), Expect = 7.565e-28
Identity = 62/174 (35.63%), Postives = 104/174 (59.77%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE-----EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            ++ +F++IWK FD D +G+IE  EL+NF  E  K  +    +S+     EK+ E+    +Q +D N DGK++++E+A++LP +EN+L    F+     ++ +    +  YD D++G IE  EL+GFL DLL      YD+  L+ + Q IL  +D+N DGK+ ++E+  +L  Q
Sbjct:   17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEF----MQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183          
BLAST of Calbindin-32 vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 49.2914 bits (116), Expect = 4.801e-7
Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query:  168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
            +F E +S +D DG+G I   EL +     ++  LG++    EL         +D ++  D + +  I+  E            ++ RK    DS  E  + ++ FDKD +GFI A EL++ ++  L E   +E++ E         M++  D + DG++   E   ++  K
Sbjct:   12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTTK 149          
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0340072)

HSP 1 Score: 347.436 bits (890), Expect = 8.141e-119
Identity = 170/295 (57.63%), Postives = 223/295 (75.59%), Query Frame = 3
Query:   84 KMSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALV-QEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
            ++   K  NF+ QY+D E+++L+  +A QF +VW+HYD DGNGYIEG+ELD FL EF+ S    DI+P+ +    L +LK  F++A+D N D KI++ ELAQ+LP +E FLLLFR D PL+SSVEFMKIW+++D D SG+IEADELKNFL + LKEA+    +SE+KLIEYT TMLQ+FD+NKDG+LQLSEMA+LLPVKEN+LCR +FK A K+T  DIE VF LYDRD +GTIENEEL+GFLKDLL LV ++DYD  DL  F + I+     +  GKIS  EL M+L+  ++++
Sbjct:   11 RVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMILLTLAKIS 305          
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0087056)

HSP 1 Score: 347.436 bits (890), Expect = 8.141e-119
Identity = 170/295 (57.63%), Postives = 223/295 (75.59%), Query Frame = 3
Query:   84 KMSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALV-QEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
            ++   K  NF+ QY+D E+++L+  +A QF +VW+HYD DGNGYIEG+ELD FL EF+ S    DI+P+ +    L +LK  F++A+D N D KI++ ELAQ+LP +E FLLLFR D PL+SSVEFMKIW+++D D SG+IEADELKNFL + LKEA+    +SE+KLIEYT TMLQ+FD+NKDG+LQLSEMA+LLPVKEN+LCR +FK A K+T  DIE VF LYDRD +GTIENEEL+GFLKDLL LV ++DYD  DL  F + I+     +  GKIS  EL M+L+  ++++
Sbjct:   11 RVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMILLTLAKIS 305          
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304964)

HSP 1 Score: 48.1358 bits (113), Expect = 1.389e-6
Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query:  168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
            +F E +S +D DG+G I   EL +     ++  LG++    EL         +D ++  D + +  I+  E            ++ RK    DS  E  + ++ FDKD +GFI A EL++ ++  L E   +E++ E         M++  D + DG++   E   ++  K
Sbjct:   12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTSK 149          
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0088001)

HSP 1 Score: 48.1358 bits (113), Expect = 1.389e-6
Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query:  168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
            +F E +S +D DG+G I   EL +     ++  LG++    EL         +D ++  D + +  I+  E            ++ RK    DS  E  + ++ FDKD +GFI A EL++ ++  L E   +E++ E         M++  D + DG++   E   ++  K
Sbjct:   12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTSK 149          
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304963)

HSP 1 Score: 48.1358 bits (113), Expect = 1.389e-6
Identity = 43/171 (25.15%), Postives = 78/171 (45.61%), Query Frame = 3
Query:  168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
            +F E +S +D DG+G I   EL +     ++  LG++    EL         +D ++  D + +  I+  E            ++ RK    DS  E  + ++ FDKD +GFI A EL++ ++  L E   +E++ E         M++  D + DG++   E   ++  K
Sbjct:   12 EFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVTMMTSK 149          
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2a (calbindin 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1677])

HSP 1 Score: 218.009 bits (554), Expect = 1.284e-68
Identity = 120/272 (44.12%), Postives = 175/272 (64.34%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            L   TA+QF ++W H+DADGNGYIEG ELD+F S+  ++  G  ++P   +         +F+  FD+N D +IEMSELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SGFIEA+ELK FLS+ LK+A       ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F+   +++      +F  YD+D NG I+ +EL   LKDL     ++ D   L  ++Q I+    ++  GK+  +EL+++L +   L
Sbjct:   13 LAELTASQFLDIWKHFDADGNGYIEGKELDNFFSQLEIARRGAGVDP---SNAAFKGRMTEFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSTEFMAAWRKYDTDRSGFIEANELKGFLSDLLKKA--NRHYDDQKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGV-RLSAEQFNAIFTYYDKDGNGYIDEQELDALLKDLYEKNNKEVDSKSLTGYKQSIMA---LSDGGKLYRSELEIVLCRDPML 271          
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010])

HSP 1 Score: 214.542 bits (545), Expect = 3.184e-67
Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 3
Query:  153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            N  A  F ++W H+DAD NGYIEG ELD F    +     + + P D++  E +  +K+ F+ A+D   D ++++ ELA  +LP +E FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELKNFL +   + +  ++I   KL EYT  M+++FD NKDG+L L+++AR+L ++EN+L +     + ++    D E +F  YD  + G +E  E+ GF+KD++ LV+      DL+ FR+ +L   D+N DGKI  +EL + L
Sbjct:    7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHML-----KKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHK--KKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCL 266          

HSP 2 Score: 97.4413 bits (241), Expect = 8.573e-23
Identity = 56/175 (32.00%), Postives = 105/175 (60.00%), Query Frame = 3
Query:  435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            A LD++  F++IW+ FD D +G+IE  EL +F    LK+ +P+++I++E++ +   + +  +D+  DG+LQ+ E+A + LP +EN+L   IF+    +  +V+   ++  YD D +G I   EL+ FLKDL    ++    + L+ +   ++  +D N DG++ +N+L  +L  Q
Sbjct:    6 ANLDAA-GFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQ 177          

HSP 3 Score: 96.6709 bits (239), Expect = 1.905e-22
Identity = 58/176 (32.95%), Postives = 103/176 (58.52%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKE 683
            + +F ++W  YDAD +GYI  +EL +FL +  +    + I P++L++ T + +K      FD+N D ++++++LA++L   E FLL F+ DA   ++   +F KI+  +D  ++G +E  E+  F+ + ++  RP   IS   L ++   +L   D NKDGK+Q SE+A  L +K 
Sbjct:  103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ-HKKKIPPNKLDEYTDAMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRP--SISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270          
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010])

HSP 1 Score: 214.542 bits (545), Expect = 3.184e-67
Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 3
Query:  153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            N  A  F ++W H+DAD NGYIEG ELD F    +     + + P D++  E +  +K+ F+ A+D   D ++++ ELA  +LP +E FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELKNFL +   + +  ++I   KL EYT  M+++FD NKDG+L L+++AR+L ++EN+L +     + ++    D E +F  YD  + G +E  E+ GF+KD++ LV+      DL+ FR+ +L   D+N DGKI  +EL + L
Sbjct:    7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHML-----KKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHK--KKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCL 266          

HSP 2 Score: 97.4413 bits (241), Expect = 8.573e-23
Identity = 56/175 (32.00%), Postives = 105/175 (60.00%), Query Frame = 3
Query:  435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            A LD++  F++IW+ FD D +G+IE  EL +F    LK+ +P+++I++E++ +   + +  +D+  DG+LQ+ E+A + LP +EN+L   IF+    +  +V+   ++  YD D +G I   EL+ FLKDL    ++    + L+ +   ++  +D N DG++ +N+L  +L  Q
Sbjct:    6 ANLDAA-GFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQ 177          

HSP 3 Score: 96.6709 bits (239), Expect = 1.905e-22
Identity = 58/176 (32.95%), Postives = 103/176 (58.52%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKE 683
            + +F ++W  YDAD +GYI  +EL +FL +  +    + I P++L++ T + +K      FD+N D ++++++LA++L   E FLL F+ DA   ++   +F KI+  +D  ++G +E  E+  F+ + ++  RP   IS   L ++   +L   D NKDGK+Q SE+A  L +K 
Sbjct:  103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ-HKKKIPPNKLDEYTDAMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRP--SISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270          
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2b (calbindin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1668])

HSP 1 Score: 201.83 bits (512), Expect = 2.857e-62
Identity = 117/268 (43.66%), Postives = 175/268 (65.30%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQ 950
            L   TA+QF ++W+ +DADGNG IEG EL++F+ E  + I  +  +P   +  +  D  ++F+  FD+N D KIEMSELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SG+IE++ELK FLS+ LK+A       ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F+N  K++  + E +F+ YD+D NG I+  EL   L+DL    +   D   L   ++ I+    ++  GK+   EL+++L +
Sbjct:   13 LAELTASQFLDIWNKFDADGNGCIEGKELENFIRE--LEIARKTADP---SNASFKDRMKEFMQKFDENGDGKIEMSELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGYIESNELKGFLSDLLKKAN--RHYDDKKLNEYTQTILKMFDLNGDGKLGLSEMARLLPVEENFLLK--FQNF-KLSAEEFEAIFNYYDKDGNGYIDEHELDALLRDLYHKHKMAVDPHSLSSSKKSIMA---LSDGGKLFRTELEIVLCK 265          
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb1 (calbindin 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8107])

HSP 1 Score: 187.578 bits (475), Expect = 6.705e-57
Identity = 110/268 (41.04%), Postives = 165/268 (61.57%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKET---LSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            +A+QF +++ HYD DGNG+IEG EL +F+ E             +  K+    L+D  + F++ ++QN+D KI + EL Q+LPT+E FLL FR+   L S  EFM+ W+ +D D SG+IEADELKNFL + L+  R ++   E+KL EYT T L++FDSNKDGKL LSEMARLLP  EN+L +  F+   K+   +   +F LYD+D+NG ++  EL   LKDL     +  + + +  ++  I+    ++  GK+   EL ++L  +
Sbjct:   12 SASQFLDIFHHYDNDGNGFIEGKELQNFIKEL-----------QQARKQAGLELTDKMKAFVEKYEQNADGKIGIIELVQILPTEENFLLFFRQ--QLKSCAEFMEAWRIYDADHSGYIEADELKNFLKDLLQ--RAKKPYDEKKLEEYTLTTLKIFDSNKDGKLCLSEMARLLPDNENFLLK--FQGV-KMARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNNKVLEANKISTYKSAIMA---LSDGGKLYRTELALVLCAE 258          
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242])

HSP 1 Score: 216.853 bits (551), Expect = 5.436e-68
Identity = 113/263 (42.97%), Postives = 173/263 (65.78%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            A +F E+W  +DAD NGYIEG ELD F    +     + +  + +  E +  +K  F+ A+D  +D ++++ ELA  +LP DE FLLLFR++ PLD+SVEFM+IW+++D+D SGFI A EL+NFL +   + +  +QI+ +KL EYT TM++LF+ NKDG+L L+++A++L ++EN+L +  +  ++      D E +F  YD  K G +E  E+ GF+KD++ LV+      DL+ FRQI+LN  DVN DGKI  +EL + L
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLL-----KKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHK--KQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265          

HSP 2 Score: 83.5741 bits (205), Expect = 9.465e-18
Identity = 52/170 (30.59%), Postives = 92/170 (54.12%), Query Frame = 3
Query:  450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            + EF++IW++FD D +G+IE  EL  F    LK+      I+ EK+       +  +D   DG+LQ+ E+A + LP  EN+L   +F+    +  +V+   ++  YD D +G I   EL+ FL+DL    ++    D L+ +   ++  ++ N DG++ +N+L  +L  Q
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL-GANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLL--LFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQ 176          

HSP 3 Score: 82.0333 bits (201), Expect = 3.249e-17
Identity = 53/177 (29.94%), Postives = 98/177 (55.37%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            + +F  +W  YD D +G+I   EL +FL +  +    + I  D+L++ T + +K      F++N D ++++++LA++L   E FLL F+ DA    +   +F  I+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L   D NKDGK+Q SE+A  L +K +
Sbjct:  102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ-HKKQITGDKLDEYTDTMMK-----LFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 270          
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242])

HSP 1 Score: 211.46 bits (537), Expect = 7.429e-66
Identity = 111/262 (42.37%), Postives = 170/262 (64.89%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            A +F E+W  +DAD NGYIEG ELD F    +     + +  + +  E +  +K  F+ A+D  +D ++++ ELA  +LP DE FLLLFR++ PLD+SVEFM+IW+++D+D SGFI A EL+NFL +   + +  +QI+ +KL EYT TM++LF+ NKDG+L L+++A+ +  K ++L   ++ ++      D E +F  YD  K G +E  E+ GF+KD++ LV+      DL+ FRQI+LN  DVN DGKI  +EL + L
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLL-----KKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHK--KQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKYVFYK-DFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 263          

HSP 2 Score: 68.1662 bits (165), Expect = 2.183e-12
Identity = 45/175 (25.71%), Postives = 92/175 (52.57%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            + +F  +W  YD D +G+I   EL +FL +  +    + I  D+L++ T + +K      F++N D ++++++LA+ +   +  +      +  +   +F  I+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L   D NKDGK+Q SE+A  L +K +
Sbjct:  102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ-HKKQITGDKLDEYTDTMMK-----LFNKNKDGRLDLNDLAKYVFYKDFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 268          
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: gata5 (GATA binding protein 5 [Source:Xenbase;Acc:XB-GENE-484906])

HSP 1 Score: 189.504 bits (480), Expect = 3.521e-58
Identity = 95/185 (51.35%), Postives = 135/185 (72.97%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR 698
            L   TA+QF ++W HYD+DGNGYIEG EL++F  E   +  G  ++   +N     +  ++F+  +D+N+D +IEM+ELAQ+LPT+E FLL FR+ A   SS+EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E+KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +
Sbjct:   12 LAELTASQFLDIWKHYDSDGNGYIEGKELENFFRELEAARRGAGVDASNVN---FGEKLKEFMQKYDKNADGRIEMAELAQILPTEENFLLCFRQHA--GSSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---FDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLK 188          

HSP 2 Score: 105.531 bits (262), Expect = 3.783e-26
Identity = 61/175 (34.86%), Postives = 103/175 (58.86%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE------EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            ++ +F+ IWK +D D +G+IE  EL+NF  E L+ AR    +        EKL E+    +Q +D N DG+++++E+A++LP +EN+L    F+     ++++    +  YD D++G IE  EL+GFL DLL      +D+  L  + Q IL  +D+N DGK+ ++E+  +L  Q
Sbjct:   16 TASQFLDIWKHYDSDGNGYIEGKELENFFRE-LEAARRGAGVDASNVNFGEKLKEF----MQKYDKNADGRIEMAELAQILPTEENFLL--CFRQHAG-SSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPFDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQ 182          

HSP 3 Score: 59.3066 bits (142), Expect = 1.040e-9
Identity = 33/90 (36.67%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFR 428
            ++ +F E W  YD D +GYIE +EL  FLS+    +L +   P   +++ L +  +  L  FD N D K+ +SE++++LP  E FLL F+
Sbjct:  107 SSIEFMEAWRKYDTDRSGYIEANELKGFLSD----LLKKANRP--FDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLKFQ 190          
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969])

HSP 1 Score: 187.578 bits (475), Expect = 1.113e-56
Identity = 117/259 (45.17%), Postives = 167/259 (64.48%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNEL 932
            +A+QF E+W HYD DGNG+IEG EL +F+ E   +     ++        LSD  + F+D ++ N+D KI ++ELAQ+LPT+E FLL FR+   L SS EFM+ W+++D D SGFIE DELK+FL + LK+A +P E   E KL EYT TML +FD+N DGKL L+EM+ LLPV+EN+L +  F+   K+   +   VF LYD+D NG I+  EL   LKDL    ++D D ++L  +++ I+    ++  GK+   EL
Sbjct:   17 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQARKKAGLD--------LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFLLFFRQQ--LKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCE---ETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLK--FQGV-KMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKDLDINNLSTYKKSIMA---LSDGGKLYRTEL 256          

HSP 2 Score: 98.9821 bits (245), Expect = 2.794e-23
Identity = 57/169 (33.73%), Postives = 102/169 (60.36%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            S+ +F++IW+ +D D +GFIE  EL+NF+ E L++AR +  +    L +     +  +++N DGK+ ++E+A++LP +EN+L   +F      ++ +    +  YD D +G IE +EL+ FLKDLL    +  ++  LE +   +L  +D N+DGK+ + E+ M+L  Q
Sbjct:   17 SASQFLEIWRHYDTDGNGFIEGKELQNFILE-LQQARKKAGLD---LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFL---LFFRQQLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQ 178          
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969])

HSP 1 Score: 181.8 bits (460), Expect = 3.898e-54
Identity = 119/287 (41.46%), Postives = 170/287 (59.23%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYL-------------------CRPIFK-----NADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            +A+QF E+W HYD DGNG+IEG EL +F+ E   +     ++        LSD  + F+D ++ N+D KI ++ELAQ+LPT+E FLL FR+   L SS EFM+ W+++D D SGFIE DELK+FL + LK+A +P E   E KL EYT TML +FD+N DGKL L+EM+ LLPV+EN+L                   C  I +        K+   +   VF LYD+D NG I+  EL   LKDL    ++D D ++L  +++ I+    ++  GK+   EL ++L
Sbjct:   12 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQARKKAGLD--------LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFLLFFRQQ--LKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCE---ETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQISVNFICVGINQMCFNPQGVKMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKDLDINNLSTYKKSIM---ALSDGGKLYRTELALVL 282          

HSP 2 Score: 98.9821 bits (245), Expect = 4.544e-23
Identity = 61/186 (32.80%), Postives = 112/186 (60.22%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML-MQQSRLAKL---THLEPRQ 992
            S+ +F++IW+ +D D +GFIE  EL+NF+ E L++AR +  +    L +     +  +++N DGK+ ++E+A++LP +EN+L   +F      ++ +    +  YD D +G IE +EL+ FLKDLL    +  ++  LE +   +L  +D N+DGK+ + E+ M+L +Q++ L K    + ++P Q
Sbjct:   12 SASQFLEIWRHYDTDGNGFIEGKELQNFILE-LQQARKKAGLD---LSDQMKAFVDQYENNTDGKIGIAELAQILPTEENFL---LFFRQQLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQ 190          
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914])

HSP 1 Score: 208.764 bits (530), Expect = 6.188e-65
Identity = 118/270 (43.70%), Postives = 176/270 (65.19%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            L   TA+QF E+W H+DADGNGYIEG EL++F  E   +  G  +     N     +  ++F+  +D+NSD KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+T+ +   +F  YD+D +G I+  EL   LKDL    +++ +   L  +R+ +++   +   GK+   +L+++L  +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTTYRKSVMS---LAEAGKLYRKDLEIVLCSE 268          
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914])

HSP 1 Score: 205.297 bits (521), Expect = 5.642e-64
Identity = 112/226 (49.56%), Postives = 154/226 (68.14%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDL 821
            L   TA+QF E+W H+DADGNGYIEG EL++F  E   +  G  +     N     +  ++F+  +D+NSD KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+T+ +   +F  YD+D +G I+  EL   LKDL
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDN---FGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRP---YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDGSGYIDENELDALLKDL 227          

HSP 2 Score: 110.923 bits (276), Expect = 6.429e-28
Identity = 62/174 (35.63%), Postives = 104/174 (59.77%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISE-----EKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            ++ +F++IWK FD D +G+IE  EL+NF  E  K  +    +S+     EK+ E+    +Q +D N DGK++++E+A++LP +EN+L    F+     ++ +    +  YD D++G IE  EL+GFL DLL      YD+  L+ + Q IL  +D+N DGK+ ++E+  +L  Q
Sbjct:   17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEF----MQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183          

HSP 3 Score: 73.559 bits (179), Expect = 1.437e-14
Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFL----KEAR 557
            ++ +F E W  YD D +GYIE +EL  FLS+    +L +   P   ++  L +  +  L  FD N D K+ +SE++++LP  E FLL F+      +S EF  I+  +DKD SG+I+ +EL   L +      KEAR
Sbjct:  108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSD----LLKKANRP--YDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQ--GMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEAR 236          
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb1 (calbindin 1 [Source:MGI Symbol;Acc:MGI:88248])

HSP 1 Score: 193.356 bits (490), Expect = 3.847e-59
Identity = 112/265 (42.26%), Postives = 167/265 (63.02%), Query Frame = 3
Query:  156 FTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSI--LGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
             TA+QF E+W H+DADG+GY+EG EL + + E + +    G +++P+           + F+D + Q  D KI + ELA VLPT+E FLLLFR    L S  EFMK W+++D D SGFIE +ELKNFL + L++A   + + + KL EYT  ML+LFDSN DGKL+L+EMARLLPV+EN+L +  F+   K+   +    F LYD+D NG I+  EL   LKDL    +++ D +++  +++ I+    ++  GK+   +L ++L
Sbjct:   11 ITASQFFEIWLHFDADGSGYLEGKELQNLIQELLQARKKAGLELSPE----------MKSFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQ-QLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKAN--KTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLK--FQGI-KMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQELDINNITTYKKNIMA---LSDGGKLYRTDLALIL 256          

HSP 2 Score: 88.1965 bits (217), Expect = 1.679e-19
Identity = 55/173 (31.79%), Postives = 94/173 (54.34%), Query Frame = 3
Query:  435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            + L ++ +F +IW  FD D SG++E  EL+N + E L +AR +  +   +L     + +  +    DGK+ + E+A +LP +EN+L   +F+     +  +    +  YD D +G IE EEL+ FLKDLL    +  D   L  +  ++L  +D N+DGK+ + E+  +L  Q
Sbjct:    8 SSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELL-QARKKAGL---ELSPEMKSFVDQYGQRDDGKIGIVELAHVLPTEENFLL--LFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQ 174          
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873])

HSP 1 Score: 189.889 bits (481), Expect = 1.168e-57
Identity = 107/263 (40.68%), Postives = 162/263 (61.60%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQV-LPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            A  F ++W  +D +  GYI  +ELD+F       +L +    D L +E +  +K   + + + + + +I M ELA + L  DE FLL FR + PLD+SVEFM+IW+++D D SGFI A EL NFL +     +  + ISE +L EYTSTM+++FD NKDG+L L+++AR+L ++EN+L +  +  ++ +    D E +F  YD  K G +E  E+ GF+KD++ LVQ      DL+ FR+I+L   DVN DGKI  +EL + L
Sbjct:   14 AACFWQIWQRFDKEEKGYIRETELDAFFDH----LLAKSGTEDTLMEENVQKVKEQLMTSHNVSKEGRILMKELASMFLSEDENFLLFFRLETPLDNSVEFMQIWRKYDADSSGFISAAELCNFLRDLFLHHK--KNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 270          

HSP 2 Score: 90.5077 bits (223), Expect = 2.839e-20
Identity = 60/180 (33.33%), Postives = 104/180 (57.78%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVE-----FMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            + +F ++W  YDAD +G+I  +EL +FL +  +    ++I+  EL + T + +K      FD+N D ++++++LA++L   E FLL F+ DA   SS E     F KI+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L+  D NKDGK+Q SE+A  L +K N
Sbjct:  107 SVEFMQIWRKYDADSSGFISAAELCNFLRDLFLH-HKKNISEAELEEYTSTMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDA---SSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQP--SISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 275          

HSP 3 Score: 73.9442 bits (180), Expect = 1.667e-14
Identity = 49/175 (28.00%), Postives = 96/175 (54.86%), Query Frame = 3
Query:  435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            A LD++  F +IW++FDK++ G+I   EL  F    L ++  E+ + EE + +    ++   + +K+G++ + E+A + L   EN+L    F+    +  +V+   ++  YD D +G I   EL  FL+DL    +++  + +LE +   ++  +D N DG++ +N+L  +L  Q
Sbjct:   10 ARLDAAC-FWQIWQRFDKEEKGYIRETELDAFFDHLLAKSGTEDTLMEENVQKVKEQLMTSHNVSKEGRILMKELASMFLSEDENFLL--FFRLETPLDNSVEFMQIWRKYDADSSGFISAAELCNFLRDLFLHHKKNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQ 181          
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873])

HSP 1 Score: 88.1965 bits (217), Expect = 7.442e-21
Identity = 50/116 (43.10%), Postives = 74/116 (63.79%), Query Frame = 3
Query:  609 LQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITT----VDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            +++FD NKDG+L L+++AR+L ++EN+L +  FK  D  +T     D E +F  YD  K G +E  E+ GF+KD++ LVQ      DL+ FR+I+L   DVN DGKI  +EL + L
Sbjct:    1 MKIFDKNKDGRLDLNDLARILALQENFLLQ--FK-MDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 113          

HSP 2 Score: 67.3958 bits (163), Expect = 1.795e-13
Identity = 43/120 (35.83%), Postives = 71/120 (59.17%), Query Frame = 3
Query:  342 FDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVE-----FMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            FD+N D ++++++LA++L   E FLL F+ DA   SS E     F KI+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L+  D NKDGK+Q SE+A  L +K N
Sbjct:    4 FDKNKDGRLDLNDLARILALQENFLLQFKMDA---SSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQP--SISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 118          
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P41044|CAB32_DROME (Calbindin-32 OS=Drosophila melanogaster OX=7227 GN=Cbp53E PE=2 SV=1)

HSP 1 Score: 347.436 bits (890), Expect = 8.160e-118
Identity = 170/295 (57.63%), Postives = 223/295 (75.59%), Query Frame = 3
Query:   84 KMSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALV-QEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
            ++   K  NF+ QY+D E+++L+  +A QF +VW+HYD DGNGYIEG+ELD FL EF+ S    DI+P+ +    L +LK  F++A+D N D KI++ ELAQ+LP +E FLLLFR D PL+SSVEFMKIW+++D D SG+IEADELKNFL + LKEA+    +SE+KLIEYT TMLQ+FD+NKDG+LQLSEMA+LLPVKEN+LCR +FK A K+T  DIE VF LYDRD +GTIENEEL+GFLKDLL LV ++DYD  DL  F + I+     +  GKIS  EL M+L+  ++++
Sbjct:   11 RVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMILLTLAKIS 305          
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q0VFG3|SEGN_XENTR (Secretagogin OS=Xenopus tropicalis OX=8364 GN=scgn PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 2.880e-67
Identity = 113/263 (42.97%), Postives = 173/263 (65.78%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            A +F E+W  +DAD NGYIEG ELD F    +     + +  + +  E +  +K  F+ A+D  +D ++++ ELA  +LP DE FLLLFR++ PLD+SVEFM+IW+++D+D SGFI A EL+NFL +   + +  +QI+ +KL EYT TM++LF+ NKDG+L L+++A++L ++EN+L +  +  ++      D E +F  YD  K G +E  E+ GF+KD++ LV+      DL+ FRQI+LN  DVN DGKI  +EL + L
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLL-----KKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHK--KQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265          

HSP 2 Score: 83.5741 bits (205), Expect = 5.014e-17
Identity = 52/170 (30.59%), Postives = 92/170 (54.12%), Query Frame = 3
Query:  450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            + EF++IW++FD D +G+IE  EL  F    LK+      I+ EK+       +  +D   DG+LQ+ E+A + LP  EN+L   +F+    +  +V+   ++  YD D +G I   EL+ FL+DL    ++    D L+ +   ++  ++ N DG++ +N+L  +L  Q
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL-GANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLL--LFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQ 176          

HSP 3 Score: 82.0333 bits (201), Expect = 1.721e-16
Identity = 53/177 (29.94%), Postives = 98/177 (55.37%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            + +F  +W  YD D +G+I   EL +FL +  +    + I  D+L++ T + +K      F++N D ++++++LA++L   E FLL F+ DA    +   +F  I+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L   D NKDGK+Q SE+A  L +K +
Sbjct:  102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ-HKKQITGDKLDEYTDTMMK-----LFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 270          
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q63ZJ3|SEGN_XENLA (Secretagogin OS=Xenopus laevis OX=8355 GN=scgn PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 8.194e-67
Identity = 114/263 (43.35%), Postives = 172/263 (65.40%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            A +F E+W  +DAD NGYIEG ELD F    +  +    I  D++       +K  F+ A+D  +D ++++ ELA  +LP DE FLLLFR++ PLD+SVEFM+IW++FD+D SGFI A EL+NFL +   + +  + I+ +KL EYT TM++LF+ NKDG+L L+++A++L ++EN+L +  +  ++ +    D E +F  YD  K G +E  E+ GF+KD++ LV+      DL+ FRQI+LN  DVN DGKI  +EL + L
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKLGTNAITADKVQG-----VKDRFMSAYDITADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKFDEDSSGFISAVELRNFLQDLFLQHK--KHITGDKLDEYTDTMMKLFNRNKDGRLDLNDLAKILALQENFLLQFKMDASSQEERKSDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265          

HSP 2 Score: 82.8037 bits (203), Expect = 1.179e-16
Identity = 53/177 (29.94%), Postives = 98/177 (55.37%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPL--DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN 686
            + +F  +W  +D D +G+I   EL +FL +  +    + I  D+L++ T + +K      F++N D ++++++LA++L   E FLL F+ DA    +   +F  I+  +D  ++G +E  E+  F+ + ++  +P   IS   L ++   +L   D NKDGK+Q SE+A  L +K N
Sbjct:  102 SVEFMRIWRKFDEDSSGFISAVELRNFLQDLFLQ-HKKHITGDKLDEYTDTMMK-----LFNRNKDGRLDLNDLAKILALQENFLLQFKMDASSQEERKSDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKP--SISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKAN 270          

HSP 3 Score: 79.7221 bits (195), Expect = 1.298e-15
Identity = 50/170 (29.41%), Postives = 92/170 (54.12%), Query Frame = 3
Query:  450 SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            + EF++IW++FD D +G+IE  EL  F    LK+      I+ +K+       +  +D   DG+LQ+ E+A + LP  EN+L   +F+    +  +V+   ++  +D D +G I   EL+ FL+DL    ++    D L+ +   ++  ++ N DG++ +N+L  +L  Q
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL-GTNAITADKVQGVKDRFMSAYDITADGRLQIQELANMILPEDENFLL--LFRRETPLDNSVEFMRIWRKFDEDSSGFISAVELRNFLQDLFLQHKKHITGDKLDEYTDTMMKLFNRNKDGRLDLNDLAKILALQ 176          
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P07090|CALB2_CHICK (Calretinin OS=Gallus gallus OX=9031 GN=CALB2 PE=2 SV=3)

HSP 1 Score: 214.542 bits (545), Expect = 2.289e-66
Identity = 120/273 (43.96%), Postives = 181/273 (66.30%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-RPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            L   +A+QF +VW H+DADGNGYIEG EL++F  E   +  G  ++     +++L D  ++F+  +D+N+D KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK FLS+ LK+A RP     E KL EYT T+L++FD N DGKL LSEM+RLLPV+EN+L +  F+   K+++ +   +F  YD+D +G I+  EL   LKDL    +++     L  +R+ I+N   ++  GK+   EL+++L  +  L
Sbjct:   11 LAELSASQFLDVWRHFDADGNGYIEGKELENFFQELESARKGTGVDS---KRDSLGDKMKEFMHKYDKNADGKIEMAELAQILPTEENFLLCFRQH--VGSSSEFMEAWRRYDTDRSGYIEANELKGFLSDLLKKANRP---YDEAKLQEYTQTILRMFDMNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLSSEEFNAIFAFYDKDGSGFIDEHELDALLKDLYEKNKKEMSIQQLTNYRRSIMN---LSDGGKLYRKELEVVLCSEPPL 269          
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q5XJX1|SEGN_DANRE (Secretagogin OS=Danio rerio OX=7955 GN=scgn PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 2.404e-66
Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 3
Query:  153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            N  A  F ++W H+DAD NGYIEG ELD F    +     + + P D++  E +  +K+ F+ A+D   D ++++ ELA  +LP +E FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELKNFL +   + +  ++I   KL EYT  M+++FD NKDG+L L+++AR+L ++EN+L +     + ++    D E +F  YD  + G +E  E+ GF+KD++ LV+      DL+ FR+ +L   D+N DGKI  +EL + L
Sbjct:    7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHML-----KKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHK--KKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCL 266          

HSP 2 Score: 97.4413 bits (241), Expect = 6.472e-22
Identity = 56/175 (32.00%), Postives = 105/175 (60.00%), Query Frame = 3
Query:  435 APLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            A LD++  F++IW+ FD D +G+IE  EL +F    LK+ +P+++I++E++ +   + +  +D+  DG+LQ+ E+A + LP +EN+L   IF+    +  +V+   ++  YD D +G I   EL+ FLKDL    ++    + L+ +   ++  +D N DG++ +N+L  +L  Q
Sbjct:    6 ANLDAA-GFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQ 177          

HSP 3 Score: 96.6709 bits (239), Expect = 1.438e-21
Identity = 58/176 (32.95%), Postives = 103/176 (58.52%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKE 683
            + +F ++W  YDAD +GYI  +EL +FL +  +    + I P++L++ T + +K      FD+N D ++++++LA++L   E FLL F+ DA   ++   +F KI+  +D  ++G +E  E+  F+ + ++  RP   IS   L ++   +L   D NKDGK+Q SE+A  L +K 
Sbjct:  103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ-HKKKIPPNKLDEYTDAMMK-----IFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRP--SISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270          
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267G6Z8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033745g2 PE=4 SV=1)

HSP 1 Score: 422.55 bits (1085), Expect = 4.364e-145
Identity = 199/287 (69.34%), Postives = 247/287 (86.06%), Query Frame = 3
Query:  105 TNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
             NFLAQ++D  T++L+NFTATQFAEVWSHYD DGNG+IEG ELD FL EFI S++ EDI P+ +++  L+ +KRDF+DAFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IW+QFDKD SG+IEADEL++FL   L  A  +  +SE+KL+EYTSTMLQLFD N+DGKLQLSEMA+LLPVKENYLCRPIFKNA++I + DI+ VF+LYDRDKNG IENEELQGFLKDLL L QEDYD  D+ FF+++IL++WDVNHDGKIS +ELKM+L+QQSRLA
Sbjct:   14 ANFLAQFRDQNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDIGPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCA--DRCVSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQEDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMILLQQSRLA 298          
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A1I8GV54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003004g1 PE=4 SV=1)

HSP 1 Score: 422.55 bits (1085), Expect = 4.509e-145
Identity = 199/287 (69.34%), Postives = 247/287 (86.06%), Query Frame = 3
Query:  105 TNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
             NFLAQ++D  T++L+NFTATQFAEVWSHYD DGNG+IEG ELD FL EFI S++ EDI P+ +++  L+ +KRDF+DAFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IW+QFDKD SG+IEADEL++FL   L  A  +  +SE+KL+EYTSTMLQLFD N+DGKLQLSEMA+LLPVKENYLCRPIFKNA++I + DI+ VF+LYDRDKNG IENEELQGFLKDLL L QEDYD  D+ FF+++IL++WDVNHDGKIS +ELKM+L+QQSRLA
Sbjct:   14 ANFLAQFRDQNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDIGPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCA--DRCVSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQEDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMILLQQSRLA 298          
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GD68 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g2 PE=4 SV=1)

HSP 1 Score: 421.009 bits (1081), Expect = 1.863e-144
Identity = 204/300 (68.00%), Postives = 250/300 (83.33%), Query Frame = 3
Query:   87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE-------DYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
            MS  K  NFL+Q++D  TKKL+NFTATQFAEVWSHYD DGNG+IEG+ELD FL EFI S++ ED   + +++  ++ +KRDF++AFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IWKQFDKD SGFIEADELK+FL   L  A  ++ ++E+KLIEYT TMLQLFD N+DGKLQLSEMARLLPVKENYLCRPIFKNA KI++ DI+ VF LYDRDKNGTIENEELQGFLKDLL L QE       DYD  D++FF+++IL++WDVNHDGKIS +ELKM+LMQQSR+A
Sbjct:    1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKRDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSA--DKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGMTQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRIA 298          
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GV22 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g1 PE=4 SV=1)

HSP 1 Score: 419.468 bits (1077), Expect = 7.226e-144
Identity = 203/300 (67.67%), Postives = 250/300 (83.33%), Query Frame = 3
Query:   87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE-------DYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
            MS  K  NFL+Q++D  TKKL+NFTATQFAEVWSHYD DGNG+IEG+ELD FL EFI S++ ED   + +++  ++ +K+DF++AFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IWKQFDKD SGFIEADELK+FL   L  A  ++ ++E+KLIEYT TMLQLFD N+DGKLQLSEMARLLPVKENYLCRPIFKNA KI++ DI+ VF LYDRDKNGTIENEELQGFLKDLL L QE       DYD  D++FF+++IL++WDVNHDGKIS +ELKM+LMQQSR+A
Sbjct:    1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSA--DKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGMTQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRIA 298          
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267FNU4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g3 PE=4 SV=1)

HSP 1 Score: 419.468 bits (1077), Expect = 7.801e-144
Identity = 203/300 (67.67%), Postives = 250/300 (83.33%), Query Frame = 3
Query:   87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE-------DYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLA 965
            MS  K  NFL+Q++D  TKKL+NFTATQFAEVWSHYD DGNG+IEG+ELD FL EFI S++ ED   + +++  ++ +K+DF++AFD+N D KIE+ EL+Q+LPT+E FLLLFR++ P+DSSVEFM+IWKQFDKD SGFIEADELK+FL   L  A  ++ ++E+KLIEYT TMLQLFD N+DGKLQLSEMARLLPVKENYLCRPIFKNA KI++ DI+ VF LYDRDKNGTIENEELQGFLKDLL L QE       DYD  D++FF+++IL++WDVNHDGKIS +ELKM+LMQQSR+A
Sbjct:    1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSA--DKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGITQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRIA 298          
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:103030695])

HSP 1 Score: 220.705 bits (561), Expect = 1.035e-69
Identity = 118/271 (43.54%), Postives = 176/271 (64.94%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINP-DELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFK-NADKI--TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            L N  A  F ++W H+DAD NGYIEG ELD+F    +  +      P D + +E +  LK  F+ A+D  +D ++++ ELA  +LP DE FLL+FR++APLD+SVEFMKIW+++D D SG+I A ELK+FL +  +  + E  +S  KL EYT TM+ +FD NKDG+L L+++AR+L ++EN+L +  FK +A+ +     D E +F  YD  K G +E  E+ GF+KD++ LV+      +L+ FR+++L   D+N DGKI  NEL + L
Sbjct:    5 LDNLDAAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKL-----RPGDNVTEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLLVFRREAPLDNSVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKKE--LSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQ--FKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRPSISGSELDQFRKVLLRHCDINKDGKIQKNELALCL 266          

HSP 2 Score: 97.8265 bits (242), Expect = 5.277e-23
Identity = 56/178 (31.46%), Postives = 100/178 (56.18%), Query Frame = 3
Query:  432 DAPLDS--SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL-LPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            D  LD+  +  F++IW+ FD D +G+IE  EL  F    + + RP + ++EEK+ +  +  +  +D   DG+LQ+ E+A + LP  EN+L   +F+    +  +V+   ++  YD D +G I   EL+ FL+DL    +++     LE +   ++N +D N DG++ +N+L  +L  Q
Sbjct:    2 DCDLDNLDAAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKLRPGDNVTEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLL--VFRREAPLDNSVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQ 177          

HSP 3 Score: 97.8265 bits (242), Expect = 6.130e-23
Identity = 57/175 (32.57%), Postives = 99/175 (56.57%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAP--LDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
            + +F ++W  YDAD +GYI   EL SFL +          +  EL+   L +     ++ FD+N D ++++++LA++L   E FLL F+ DA   L+   +F KI+  +D  ++G +E  E+  F+ + ++  RP   IS  +L ++   +L+  D NKDGK+Q +E+A  L +K
Sbjct:  103 SVEFMKIWRKYDADSSGYISAVELKSFLQDLFQH------HKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQFKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRP--SISGSELDQFRKVLLRHCDINKDGKIQKNELALCLGMK 269          
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2a (calretinin [Source:NCBI gene;Acc:103033342])

HSP 1 Score: 218.394 bits (555), Expect = 8.490e-69
Identity = 120/272 (44.12%), Postives = 179/272 (65.81%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            L   TA+QF ++W H+D DGNGYIEG EL++F  E  V+  G  ++P  +   +  +  +DF+  FD+N D +IEMSELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SG+IEA+ELK FLS+ LK+A       ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F+   K+T      +F  YD+D NG I+ +EL   L+DL    +++ D  DL  +++ I+    ++  GK+  +EL+++L ++  L
Sbjct:   13 LAELTASQFLDIWKHFDTDGNGYIEGKELENFFRELEVARRGAGVDPTNV---SFREKMKDFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSSEFMAAWRRYDTDRSGYIEANELKGFLSDLLKKA--NRHYDDKKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGI-KLTAEQFNAIFTYYDKDGNGYIDEQELDALLRDLYEKNKKEVDTKDLTGYKKSIMA---LSDGGKLYRSELEIVLCREPML 271          
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108])

HSP 1 Score: 198.749 bits (504), Expect = 3.450e-61
Identity = 124/269 (46.10%), Postives = 176/269 (65.43%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            L   TA+QF +VW H+D+DGNG+IEG EL+ FL E   +  G  +N + +N  +L     DF+  FDQNSD KIEM ELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SGFIEA+ELK FLS+ L +A    Q  ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F++  K+++ +   +F  YD+D NG I+  EL   LKDL      D+D   L   ++ I+    ++  GK+   EL+++L ++
Sbjct:   10 LAELTASQFMDVWRHFDSDGNGFIEGQELEDFLEELESARKG--VNAEPVNA-SLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKAN--RQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQSF-KLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKHNMDFDSQTLASSKKSIMA---LSDGGKLFRTELEIVLCKE 265          
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108])

HSP 1 Score: 197.208 bits (500), Expect = 1.280e-60
Identity = 124/269 (46.10%), Postives = 174/269 (64.68%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            L   TA+QF +VW H+D+DGNG+IEG EL+ FL E   +  G  +NP      +L     DF+  FDQNSD KIEM ELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SGFIEA+ELK FLS+ L +A    Q  ++KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F++  K+++ +   +F  YD+D NG I+  EL   LKDL      D+D   L   ++ I+    ++  GK+   EL+++L ++
Sbjct:   16 LAELTASQFMDVWRHFDSDGNGFIEGQELEDFLEELESARKG--VNP---VNASLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKA--NRQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQSF-KLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKHNMDFDSQTLASSKKSIMA---LSDGGKLFRTELEIVLCKE 269          
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb1 (calbindin 1 [Source:NCBI gene;Acc:103033937])

HSP 1 Score: 188.348 bits (477), Expect = 2.661e-57
Identity = 109/268 (40.67%), Postives = 168/268 (62.69%), Query Frame = 3
Query:  159 TATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKET---LSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            +A+QF +++ HYD DGNGYIEG EL +F+ E             +  K+    L+D  R F++ +++N+D KI++ EL Q+LPT+E FLL FR+   L S  EFM+ W+ +D D SG+IE+DELKNFL + L+  R ++   E+KL EYT T L++FDSN DGKL LSEMARLLP  EN+L +  F+   K+   +   +F LYD+D+NG ++  EL   LKDL    ++  +   ++ +++ I+    ++  GK+   EL ++L ++
Sbjct:   12 SASQFLDIFHHYDNDGNGYIEGKELQNFIKEL-----------QQARKQAGLELTDQMRAFVEEYEKNADGKIDIVELVQILPTEENFLLFFRQ--QLKSCTEFMEAWRTYDADHSGYIESDELKNFLRDLLQ--RAKKPFDEKKLEEYTLTTLKIFDSNNDGKLCLSEMARLLPDDENFLLK--FEGV-KMARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNKKVLEIGKIQVYKKAIMA---LSDGGKLYRTELALVLCEE 258          
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010])

HSP 1 Score: 204.527 bits (519), Expect = 4.475e-64
Identity = 111/258 (43.02%), Postives = 162/258 (62.79%), Query Frame = 3
Query:  177 EVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA-QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADK-ITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            EVW  +DAD NGYIEG ELD F    +  +  ++   DE     +  ++R+ +  +D+++D ++ +SE+A  +L  DE FLL+F++ + LD+SVEF+KIW Q+D D SGFI A ELKNFL + L  +    Q+ E+KL EYT TM++LFD NKDG+L L++MAR+L +KENYL +   ++A +     D E +F  YD  + G +E  E+ GF+KD++ LV+      DL  FR  +L   D N DGKI   EL + L
Sbjct:   16 EVWQKFDADDNGYIEGKELDEFFKHLLKQLAAKECVSDE----KVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLLIFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLL--SHHGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVADLSKFRDSLLRRCDTNQDGKIQKWELALCL 267          

HSP 2 Score: 95.9005 bits (237), Expect = 5.545e-23
Identity = 54/164 (32.93%), Postives = 97/164 (59.15%), Query Frame = 3
Query:  459 FMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK-ENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
             +++W++FD D +G+IE  EL  F    LK+   +E +S+EK+      ++ ++D + DG+L LSE+A +L  + E++L   IFK A  +  +V+   ++H YD D +G I   EL+ FLKDLL+      ++  LE +   ++  +D N DG++ +N++  +L
Sbjct:   14 LLEVWQKFDADDNGYIEGKELDEFFKHLLKQLAAKECVSDEKVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLL--IFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLLSHHGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARIL 175          

HSP 3 Score: 83.1889 bits (204), Expect = 1.805e-18
Identity = 50/178 (28.09%), Postives = 104/178 (58.43%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFR-KDAPL-DSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENY 689
            + +F ++W  YD D +G+I  SEL +FL + ++S  G  +   +L + T + +K      FD+N D +++++++A++L   E +LL F  +DA   +   +F KI+  +D  ++G +E  E+  F+ + ++  RP   +++  L ++  ++L+  D+N+DGK+Q  E+A  L ++  +
Sbjct:  104 SVEFIKIWHQYDLDSSGFISASELKNFLKD-LLSHHGRQVEEKKLEEYTDTMMK-----LFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVAD--LSKFRDSLLRRCDTNQDGKIQKWELALCLGIRTAH 273          
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 101.293 bits (251), Expect = 2.704e-26
Identity = 62/146 (42.47%), Postives = 96/146 (65.75%), Query Frame = 3
Query:  519 LKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQS 956
            L++FL + LK+A  E    E+KL EYT T L++FDSN+DGKL+LSEMARLLPV+EN+L +  F+    +T  +  ++F  YD+D+NG I+ +EL+  L+DL    ++D D   L  +++ I+   D    GK+  N+L+M+L  +S
Sbjct:    1 LQDFLKDLLKKANRE--FDEKKLNEYTQTTLKMFDSNRDGKLELSEMARLLPVQENFLLK--FQGV-TMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNRKDLDAQTLTSYKRNIMALSD---HGKLYRNDLEMVLCVES 138          

HSP 2 Score: 59.6918 bits (143), Expect = 2.452e-11
Identity = 41/129 (31.78%), Postives = 74/129 (57.36%), Query Frame = 3
Query:  291 ELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPV 677
            E +++ L++  +  L  FD N D K+E+SE+A++LP  E FLL F+      ++ EF  I+  +DKD++G+I+ DEL+  L +  ++ R +  +  + L  Y   ++ L D    GKL  +++  +L V
Sbjct:   15 EFDEKKLNEYTQTTLKMFDSNRDGKLELSEMARLLPVQENFLLKFQ--GVTMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNRKD--LDAQTLTSYKRNIMALSDH---GKLYRNDLEMVLCV 136          
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000059.1 (pep scaffold:Pmarinus_7.0:GL485405:3181:11335:1 gene:ENSPMAG00000000055.1 transcript:ENSPMAT00000000059.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.5001 bits (210), Expect = 3.118e-21
Identity = 39/78 (50.00%), Postives = 60/78 (76.92%), Query Frame = 3
Query:  291 ELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELK 524
            EL +  + +  + F++ +D+N+D KIEM+ELAQ+LPT+E FLL FR+   + SS EFM+ W+++D D+SG+IEA+ELK
Sbjct:    2 ELPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFLLFFRQH--VKSSAEFMETWRRYDTDRSGYIEANELK 77          

HSP 2 Score: 47.3654 bits (111), Expect = 1.261e-7
Identity = 24/80 (30.00%), Postives = 49/80 (61.25%), Query Frame = 3
Query:  564 EQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQ 803
            +++ E  + E   + ++ +D N DGK++++E+A++LP +EN+L    F+   K ++ +    +  YD D++G IE  EL+
Sbjct:    1 QELPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFLL--FFRQHVK-SSAEFMETWRRYDTDRSGYIEANELK 77          
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001806.1 (pep scaffold:Pmarinus_7.0:GL476330:305753:307416:1 gene:ENSPMAG00000001642.1 transcript:ENSPMAT00000001806.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.151 bits (139), Expect = 1.200e-11
Identity = 27/48 (56.25%), Postives = 39/48 (81.25%), Query Frame = 3
Query:  324 RDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMK 467
            ++F++ FDQNSD +IE++ELAQ+LPT+E FLL FR+   + SS EFM+
Sbjct:    7 KEFMEKFDQNSDGRIELAELAQILPTEENFLLFFRQH--VKSSAEFME 52          
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 48.521 bits (114), Expect = 2.100e-7
Identity = 42/172 (24.42%), Postives = 79/172 (45.93%), Query Frame = 3
Query:  165 TQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
             +F E +S +D DG+G I   EL +     ++  LG++    EL         +D ++  D + +  I+  E            ++ RK    DS  E  + ++ FDKD +G+I A EL++ ++  L E   +E++ E         M++  D + DG++   E  +++  K
Sbjct:   11 AEFKEAFSLFDKDGDGTITTKELGT-----VMRSLGQNPTEAEL---------QDMINEVDADGNGTIDFPEF---------LTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTN-LGEKLTDEEVDE---------MIREADIDGDGQVNYEEFVQMMTAK 149          
BLAST of Calbindin-32 vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 43.8986 bits (102), Expect = 5.958e-6
Identity = 36/124 (29.03%), Postives = 59/124 (47.58%), Query Frame = 3
Query:  165 TQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLS 536
             +F E ++ +D D NG I  SEL +     ++  LG   +  E+N         D ++  D + +++IE SE            L+ R+    DS  E ++ +K FDK+  G I A ELK+ L+
Sbjct:   11 AEFKEAFALFDKDNNGSISSSELAT-----VMRSLGLSPSEAEVN---------DLMNEIDVDGNHQIEFSEF---------LALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLT 111          
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45967 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ7])

HSP 1 Score: 159.073 bits (401), Expect = 2.083e-46
Identity = 96/269 (35.69%), Postives = 154/269 (57.25%), Query Frame = 3
Query:  144 KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQED--YDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            K    +  +F +V+  +D+D NGYIE  E+D F+ + +     +++N  E  K  ++D K++ L+ +D + D +  M EL  +LP +   L  +  +     SV+FMKI+ +FD+D+SGF+E  EL+ F  E L   + +  I+  KL +Y    L+ +D+NKDGK  L+E+A++LP++EN      +     +T  D   V+  YD+D +G IE +E+   L D+L   Q D  Y+K DLE FR IIL  +D N D K+S +EL  +L
Sbjct:    4 KATKLSPVEFVKVFMKFDSDKNGYIEEKEMDLFIHDLL-----KELNDKEPTKAEVADKKKEILEKYDLDKDGRFSMDELENILPVEPNLLRTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLS-MKGKSSINPRKLDDYIEYCLEKYDANKDGKFNLNELAKMLPLEENIF--ETYGIKRDMTREDFNKVWDYYDKDNSGEIEGDEILALLHDVLKQQQPDREYNKPDLERFRDIILEIYDENKDNKLSRDELCCLL 264          
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45965 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ5])

HSP 1 Score: 126.716 bits (317), Expect = 4.981e-35
Identity = 81/178 (45.51%), Postives = 117/178 (65.73%), Query Frame = 3
Query:  156 FTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENY 689
             T+ QF +++  YDADGN  I+  ELD FL         +D+   + N   L  LK + L+ +D N D  I+MSELAQ LP +E FL+ FRKD  + SSV+FMKI+K++D D+SG+IE  ELK FL + L   +  + IS  ++ +Y S M+++FD NKDGKL+LSE++RLL V++N+
Sbjct:   22 LTSEQFYDIFKKYDADGNDCIDAKELDLFL---------QDVQKADPNATNLDALKNEILEKYDANYDGVIQMSELAQCLPLEENFLVGFRKDNVI-SSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLDADK--KAISPSRINDYASAMIEIFDRNKDGKLELSELSRLLQVEDNF 187          

HSP 2 Score: 79.337 bits (194), Expect = 1.775e-17
Identity = 53/156 (33.97%), Postives = 91/156 (58.33%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGK 914
            +S +F  I+K++D D +  I+A EL  FL + +++A P    +   L    + +L+ +D+N DG +Q+SE+A+ LP++EN+L    F+  + I++VD   +F  YD DK+G IE  EL+ FL DLL   ++      +  +   ++  +D N DGK
Sbjct:   23 TSEQFYDIFKKYDADGNDCIDAKELDLFLQD-VQKADP----NATNLDALKNEILEKYDANYDGVIQMSELAQCLPLEENFLVG--FRKDNVISSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLDADKKAISPSRINDYASAMIEIFDRNKDGK 171          
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45966 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ6])

HSP 1 Score: 128.642 bits (322), Expect = 6.328e-35
Identity = 86/258 (33.33%), Postives = 140/258 (54.26%), Query Frame = 3
Query:  177 EVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSE-FLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDL-EFFRQIILNEWDVNHDGKISMNELKMML 944
            E++S +D DGNG+I+  ELDSF+         +DI  +EL+++ ++  K   L   D+N+DNKI   EL +VLPT   F+  F         V+F+KIWK +DKD SGF+E  EL+ FL E F     P ++I E++  ++ + +L       DGK  + + + +  +     C  +  NA      D +  F  YD+D +G IE+ EL+   +DLL +   D  ++D+ ++ + +++ + D N D KI  +ELK  L
Sbjct:   23 EIFSKFDKDGNGFIDAKELDSFI---------KDICNNELSEDDVASFKTGLLAKLDENNDNKISKKELRKVLPTKSNFMRQFLTTKEF-CQVDFIKIWKNYDKDGSGFLEKPELQLFLEELFAVNKTPGQRIDEKEREKFQTILL-------DGKEGVDKKSLMKHLGVKAACDEVAANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLKINDADITEEDVKDYVQSVVMPDLDKNKDNKIQKDELKSYL 263          

HSP 2 Score: 61.6178 bits (148), Expect = 6.824e-11
Identity = 55/193 (28.50%), Postives = 87/193 (45.08%), Query Frame = 3
Query:  105 TNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYT-STMLQLFDSNKDGKLQLSEMARLLPVK 680
            +NF+ Q+        + F    F ++W +YD DG+G++E  EL  FL E            DE  +E    +  D  +  D+ S     M  L      DEV       +A      +F + + ++DKD SG IE+ EL+    + LK    +  I+EE + +Y  S ++   D NKD K+Q  E+   L +K
Sbjct:   90 SNFMRQFL-----TTKEFCQVDFIKIWKNYDKDGSGFLEKPELQLFLEELFAVNKTPGQRIDEKEREKFQTILLDGKEGVDKKS----LMKHLGVKAACDEV-----AANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLK--INDADITEEDVKDYVQSVVMPDLDKNKDNKIQKDELKSYLKIK 266          
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO46016 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ4])

HSP 1 Score: 76.2554 bits (186), Expect = 2.071e-15
Identity = 48/172 (27.91%), Postives = 90/172 (52.33%), Query Frame = 3
Query:  456 EFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ-EDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAK 968
             +  IW ++D ++ G +  ++LK F+++ +K     +++S + +       L   D ++ G++ L EMAR L V++N+L         K+   D   ++  YD+D  G+I+  EL   ++D+ A +Q    + DD+E ++ II+   D + DG IS  EL +   Q  + AK
Sbjct:  166 RYGTIWNRYDVNREGSLSTEQLKGFINDSMKG----QKLSSKNIGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNINPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDVFAKIQGRSPEMDDIEKWKDIIMMFADADGDGAISKEELNIFFAQGLKEAK 333          

HSP 2 Score: 48.521 bits (114), Expect = 2.568e-6
Identity = 38/166 (22.89%), Postives = 83/166 (50.00%), Query Frame = 3
Query:  168 QFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLD-SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLF-DSNKDGKLQLSEM 659
            ++  +W+ YD +  G +   +L  F+++   S+ G+     +L+ + +  L +  LDA D++   ++++ E+A+ L  ++ FL  F  +        +F +IW  +D+D  G I+  EL   + +    A+ + +  E   IE    ++ +F D++ DG +   E+
Sbjct:  166 RYGTIWNRYDVNREGSLSTEQLKGFIND---SMKGQ-----KLSSKNIGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNINPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDVF--AKIQGRSPEMDDIEKWKDIIMMFADADGDGAISKEEL 321          
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45935 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRE8])

HSP 1 Score: 55.8398 bits (133), Expect = 1.244e-9
Identity = 46/176 (26.14%), Postives = 82/176 (46.59%), Query Frame = 3
Query:  156 FTATQFAEV---WSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIW-KQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLL 671
             T  Q +E+   +  YD +G+G I   EL   + E      G+ ++ +EL         +D + A D + + K+E  E  Q++           + AP    VE MK +  +FD+D +GFI++DE+K  +  F         ++ + L +    M++  D+N DGK+   E  ++L
Sbjct:    5 LTPEQLSEIEAAFKMYDTNGDGQISAEELGQAMRE-----AGQLVSDEEL---------KDMIRAVDLDGNGKVEFKEFVQMMANQ------LGQPAP----VEEMKAYFDRFDQDGNGFIDSDEMKCLVRAFYS------NLTGDALKQQVRAMIEAADTNSDGKISFEEFVKVL 150          
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000016338.2 (calretinin [Source:NCBI gene;Acc:101174993])

HSP 1 Score: 218.009 bits (554), Expect = 1.153e-68
Identity = 118/269 (43.87%), Postives = 178/269 (66.17%), Query Frame = 3
Query:  147 LRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQ 953
            L   TA+QF ++W H+D DGNGYIEG EL++F  E   S  G  ++P   +     +  R+F+  FD+N D +IEMSELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SG+IEA+ELK FLS+ L++A       E+KL EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F+   K+T+    ++F  YD+D NG I+ +EL+  L+DL    +++ D   L  +++ I++   ++  GK+   EL+++L ++
Sbjct:   13 LAEVTASQFLDIWKHFDVDGNGYIEGKELENFFRELETSRRGAGVDP---SNPQFREKMREFMQKFDKNRDGRIEMSELAQILPTEENFLLCFRQ--FVSSSAEFMAAWRRYDTDRSGYIEANELKGFLSDLLEKA--NRHYDEQKLHEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGV-KLTSEQFNSIFTYYDKDGNGYIDEQELEALLRDLYQTNKKEIDAKSLMSYKESIMS---LSDGGKLYRGELEIVLCKE 268          
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606])

HSP 1 Score: 213.001 bits (541), Expect = 1.000e-66
Identity = 117/273 (42.86%), Postives = 180/273 (65.93%), Query Frame = 3
Query:  144 KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            +L   TA QF ++W H+D DGNGYIEG EL++F ++   +  G  ++P   +  +  +  R+F+  FD+NSD +IEMSELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SG+IE++ELK FLS+ LK+A       +++L EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F+   ++T  + +++F  YD+D NG I+ +EL   LKDL    + D D   L  +++ I+    ++  GK+   E++++L + S L
Sbjct:   12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP---SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKA--NRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGI-RLTVKEFDSLFTFYDKDGNGYIDEQELDALLKDLCDKNKMDVDATGLVDYKKSIMA---LSDGGKLYRTEMEIVLCRDSML 271          
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000006100.2 (secretagogin [Source:NCBI gene;Acc:101157482])

HSP 1 Score: 203.371 bits (516), Expect = 5.216e-63
Identity = 111/271 (40.96%), Postives = 171/271 (63.10%), Query Frame = 3
Query:  153 NFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSD-LKRDFLDAFDQNSDNKIEMSELAQ-VLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCR-PIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQS 956
            N  A  F E+W H+DAD NGYIEG ELD F    +     + + P E   E     LK  F+ A+D  +D ++++ ELA  +LP +E FLL+F ++APLD+SV+FMKIW+++D D SG+I A ELK FL +  ++   E  +S +KL EYT  M+++FD NKDG+L L+++AR+L +++N+L +  +  ++ +    D E +F  YD  K G +E  E+ GF+KD++ LV+      +L+  R ++L+  DVN+DGKI  NEL + L  +S
Sbjct:    7 NLDAAGFLEIWQHFDADDNGYIEGKELDEFFHHMM-----QRLGPQEETTEERVRELKERFMSAYDVTADGRLQIQELASMILPEEENFLLIFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHHRE--VSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMDASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELVRPSLSGPELDKLRAVLLHHCDVNNDGKIQKNELALCLGVKS 270          
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606])

HSP 1 Score: 201.83 bits (512), Expect = 1.677e-62
Identity = 115/273 (42.12%), Postives = 174/273 (63.74%), Query Frame = 3
Query:  144 KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 962
            +L   TA QF ++W H+D DGNGYIEG EL++F ++   +  G  ++P   +  +  +  R+F+  FD+NSD +IEMSELAQ+LPT+E FLL FR+   + SS EFM  W+++D D+SG+IE++ELK FLS+ LK+A       +++L EYT T+L++FD N DGKL LSEMARLLPV+EN+L +  F+    I+   +     L  +D NG I+ +EL   LKDL    + D D   L  +++ I+    ++  GK+   E++++L + S L
Sbjct:   12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP---SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKA--NRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQKRPPISLTCV-----LVYQDGNGYIDEQELDALLKDLCDKNKMDVDATGLVDYKKSIMA---LSDGGKLYRTEMEIVLCRDSML 267          

HSP 2 Score: 112.849 bits (281), Expect = 1.343e-28
Identity = 68/188 (36.17%), Postives = 110/188 (58.51%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEAR------PEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYL-CRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML-MQQSRLAKLTHLEP 986
            ++ +F+ IWK FD D +G+IE  EL+NF ++ L+ AR      P     +EK+ E+ +     FD N DG++++SE+A++LP +EN+L C   F      ++ +  N +  YD D++G IE+ EL+GFL DLL      YD   L  + Q IL  +D+N DGK+ ++E+  +L +Q++ L K     P
Sbjct:   17 TAAQFIDIWKHFDTDGNGYIEGKELENFFTQ-LESARRGAGVDPSHASFKEKMREFMAN----FDKNSDGRIEMSELAQILPTEENFLLCFREFVG----SSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKANRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQKRPP 195          
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: SCGN (secretagogin [Source:NCBI gene;Acc:101170527])

HSP 1 Score: 178.718 bits (452), Expect = 1.733e-53
Identity = 103/264 (39.02%), Postives = 158/264 (59.85%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQV-LPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLL--PVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 944
            A  F ++W ++D D NGYIE  ELD+F    ++ +  +D+  D++      +L+R    A   ++D ++ + +LA V LP +E FLLLFR++ PLD+SVEFMKIW+++D+D SG+I A+ELK FL +   + R  + I+  KL EYT TML++FD N+ GKL L+++ARLL   V     C    +N       D E +F  YD  K G +E  E+ G + D++ L +      +L  FR+ +++  D+N DGKI  NEL + L
Sbjct:   10 AAGFLQIWLYFDKDDNGYIEQKELDAFFKHMLLHLGMKDVTEDKIQ-----ELRRRC--AHRPSADGRLLIQQLASVMLPEEENFLLLFRQETPLDNSVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHR--KSITASKLEEYTVTMLKIFDKNQGGKLDLNDLARLLLGYVVTFQACSREDRNR------DFEKIFAHYDVSKTGALEGPEVDGLVMDMMELAKPSISGAELNKFRKALMSHCDINRDGKIQKNELALCL 258          

HSP 2 Score: 77.411 bits (189), Expect = 6.841e-16
Identity = 48/173 (27.75%), Postives = 97/173 (56.07%), Query Frame = 3
Query:  162 ATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVK 680
            + +F ++W  YD D +GYI  +EL  FL +  +    + I   +L + T++ LK      FD+N   K+++++LA++L     +++ F+  +  D + +F KI+  +D  ++G +E  E+   + + ++ A+P   IS  +L ++   ++   D N+DGK+Q +E+A  L +K
Sbjct:  100 SVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHR-KSITASKLEEYTVTMLK-----IFDKNQGGKLDLNDLARLLLG---YVVTFQACSREDRNRDFEKIFAHYDVSKTGALEGPEVDGLVMDMMELAKP--SISGAELNKFRKALMSHCDINRDGKIQKNELALCLGLK 261          

HSP 3 Score: 72.7886 bits (177), Expect = 3.205e-14
Identity = 53/176 (30.11%), Postives = 92/176 (52.27%), Query Frame = 3
Query:  432 DAPLDS--SVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMAR-LLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLM 947
            D  LD   +  F++IW  FDKD +G+IE  EL  F    L     ++ ++E+K+ E           + DG+L + ++A  +LP +EN+L   +F+    +  +V+   ++  YD D +G I   EL+GFL+DL    ++      LE +   +L  +D N  GK+ +N+L  +L+
Sbjct:    2 DGALDKLDAAGFLQIWLYFDKDDNGYIEQKELDAFFKHMLLHLGMKD-VTEDKIQELRRRCAH--RPSADGRLLIQQLASVMLPEEENFLL--LFRQETPLDNSVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHRKSITASKLEEYTVTMLKIFDKNQGGKLDLNDLARLLL 172          
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000055605.1 (SMESG000055605.1)

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 301/302 (99.67%), Postives = 301/302 (99.67%), Query Frame = 3
Query:   87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEPRQ 992
            MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQI EEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEPRQ
Sbjct:    1 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQILEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEPRQ 302          
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000023194.1 (SMESG000023194.1)

HSP 1 Score: 370.933 bits (951), Expect = 3.767e-128
Identity = 177/287 (61.67%), Postives = 231/287 (80.49%), Query Frame = 3
Query:  108 NFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAK 968
            NFL++++D++T++  NFTA QF EVW HYD DGNGYIEG ELD FL EFI S++   I  + ++   LSD+K DF+ A+D+N DNKIE+ ELAQ+LPTDE FLLLFR+D PL+SSV+FMKIW++FD D SG+IE+DELK FL   + E+  +E IS+EKL EY+ T+LQLFD N DGKLQLSEMARLLPVKENYL +P+FKN ++IT  DI+ VF  YD DKNGTIENEELQG LKDLL L  E YD++ ++F++++IL +WD++HDGKI  +ELKM+L+QQS++A+
Sbjct:   10 NFLSKFRDSKTRRWTNFTAKQFLEVWCHYDTDGNGYIEGEELDGFLREFISSVVPGSIGSEIISDSALSDMKEDFMAAYDENEDNKIEIGELAQMLPTDENFLLLFRRDNPLESSVDFMKIWRKFDTDNSGYIESDELKEFLRYLISESNVKE-ISDEKLEEYSYTILQLFDENGDGKLQLSEMARLLPVKENYLSKPLFKNVNRITGYDIDRVFKKYDTDKNGTIENEELQGLLKDLLELAGESYDEEKMKFYQEVILGQWDLDHDGKIGRDELKMILLQQSKMAQ 295          
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000003798.1 (SMESG000003798.1)

HSP 1 Score: 320.472 bits (820), Expect = 4.867e-108
Identity = 154/297 (51.85%), Postives = 222/297 (74.75%), Query Frame = 3
Query:   93 TTKFT-NFLAQYKDNETK-KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLK-EARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLT 974
            + KFT N+L +Y++ E   +L  FTATQF +VW+H+D D NGYIE +ELD+FL +F  S++ E I  + L++     +K+ FL+A+D+N D +IE+ EL+Q+LPTDE FL+LF+++ P+ SSVEFMKIWK+FDKD+SGFIEA+EL+ F++E LK   +  E++ E K++ +T+ ML+LFD N DGKLQLSEMARLLP+KENYL +P+  N  KI+ +++E +F  YD D+NG ++  E+Q  L DL+ L +E YD   LE F+ +IL++WDVN DGKISM ELKM+L Q S+L +++
Sbjct:   20 SQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKSAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEPYDDKKLEKFQNVILSQWDVNKDGKISMKELKMILTQHSKLGEIS 316          
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1)

HSP 1 Score: 237.654 bits (605), Expect = 1.894e-77
Identity = 117/198 (59.09%), Postives = 154/198 (77.78%), Query Frame = 3
Query:   87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDE----LNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL 668
            MS  K  NFL+Q++D  TKKL+NFTAT+FAEVW+HYD DGNGYIEG ELD FL EF+ S++ E+ + +     L++  +  LK + ++A+D+N+D +IE+ ELAQ+LP +E FLLLFR+D  L+SSV+F+K+W+ FDK+QSGFIEA+ELK FL   LKEA+P E   +EK+ EYT  +LQLFD NKDGKLQLSEMARL
Sbjct:    1 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 198          

HSP 2 Score: 103.219 bits (256), Expect = 7.702e-26
Identity = 60/176 (34.09%), Postives = 113/176 (64.20%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPE--EQISEEKLIEYTST------MLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLL--ALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMM 941
            ++ EF ++W  +DKD +G+IE DEL  FL EF+     E   + S+EK++  T+       +++ +D N DG++++ E+A++LP++EN+L   +F+  +++ ++VD   V+ ++D++++G IE  EL+ FLK LL  A   ED  K+ ++ + +IIL  +D N DGK+ ++E+  +
Sbjct:   25 TATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 198          
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1)

HSP 1 Score: 234.958 bits (598), Expect = 2.927e-76
Identity = 120/209 (57.42%), Postives = 155/209 (74.16%), Query Frame = 3
Query:   87 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPD--ELNKE-------------TLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARL 668
            MS  K  NFL+Q++D  TKKL+NFTAT+FAEVW+HYD DGNGYIEG ELD FL EF+ S++ E+ + +  ELNK               +  LK + ++A+D+N+D +IE+ ELAQ+LP +E FLLLFR+D  L+SSV+F+K+W+ FDK+QSGFIEA+ELK FL   LKEA+P E   +EK+ EYT  +LQLFD NKDGKLQLSEMARL
Sbjct:    1 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 209          

HSP 2 Score: 98.5969 bits (244), Expect = 5.123e-24
Identity = 59/187 (31.55%), Postives = 111/187 (59.36%), Query Frame = 3
Query:  447 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEA-------------RPEEQISEEKLIEYTST------MLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLL--ALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMM 941
            ++ EF ++W  +DKD +G+IE DEL  FL EF+                + E Q    K++  T+       +++ +D N DG++++ E+A++LP++EN+L   +F+  +++ ++VD   V+ ++D++++G IE  EL+ FLK LL  A   ED  K+ ++ + +IIL  +D N DGK+ ++E+  +
Sbjct:   25 TATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 209          
The following BLAST results are available for this feature:
BLAST of Calbindin-32 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SCGN2.179e-6545.39secretagogin, EF-hand calcium binding protein [Sou... [more]
CALB23.347e-6543.22calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
CALB23.347e-6543.22calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
CALB21.948e-5946.32calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
CALB21.948e-5946.32calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
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BLAST of Calbindin-32 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cmd-14.801e-725.15Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
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BLAST of Calbindin-32 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cbp53E8.141e-11957.63gene:FBgn0004580 transcript:FBtr0340072[more]
Cbp53E8.141e-11957.63gene:FBgn0004580 transcript:FBtr0087056[more]
Cam1.389e-625.15gene:FBgn0000253 transcript:FBtr0304964[more]
Cam1.389e-625.15gene:FBgn0000253 transcript:FBtr0088001[more]
Cam1.389e-625.15gene:FBgn0000253 transcript:FBtr0304963[more]
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BLAST of Calbindin-32 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
calb2a1.284e-6844.12calbindin 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1677... [more]
scgn3.184e-6741.20secretagogin, EF-hand calcium binding protein [Sou... [more]
scgn3.184e-6741.20secretagogin, EF-hand calcium binding protein [Sou... [more]
calb2b2.857e-6243.66calbindin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1668... [more]
calb16.705e-5741.04calbindin 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8107][more]
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BLAST of Calbindin-32 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scgn5.436e-6842.97secretagogin, EF-hand calcium binding protein [Sou... [more]
scgn7.429e-6642.37secretagogin, EF-hand calcium binding protein [Sou... [more]
gata53.521e-5851.35GATA binding protein 5 [Source:Xenbase;Acc:XB-GENE... [more]
stim11.113e-5645.17stromal interaction molecule 1 [Source:Xenbase;Acc... [more]
stim13.898e-5441.46stromal interaction molecule 1 [Source:Xenbase;Acc... [more]
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BLAST of Calbindin-32 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Calb26.188e-6543.70calbindin 2 [Source:MGI Symbol;Acc:MGI:101914][more]
Calb25.642e-6449.56calbindin 2 [Source:MGI Symbol;Acc:MGI:101914][more]
Calb13.847e-5942.26calbindin 1 [Source:MGI Symbol;Acc:MGI:88248][more]
Scgn1.168e-5740.68secretagogin, EF-hand calcium binding protein [Sou... [more]
Scgn7.442e-2143.10secretagogin, EF-hand calcium binding protein [Sou... [more]
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BLAST of Calbindin-32 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P41044|CAB32_DROME8.160e-11857.63Calbindin-32 OS=Drosophila melanogaster OX=7227 GN... [more]
sp|Q0VFG3|SEGN_XENTR2.880e-6742.97Secretagogin OS=Xenopus tropicalis OX=8364 GN=scgn... [more]
sp|Q63ZJ3|SEGN_XENLA8.194e-6743.35Secretagogin OS=Xenopus laevis OX=8355 GN=scgn PE=... [more]
sp|P07090|CALB2_CHICK2.289e-6643.96Calretinin OS=Gallus gallus OX=9031 GN=CALB2 PE=2 ... [more]
sp|Q5XJX1|SEGN_DANRE2.404e-6641.20Secretagogin OS=Danio rerio OX=7955 GN=scgn PE=1 S... [more]
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BLAST of Calbindin-32 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267G6Z84.364e-14569.34Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GV544.509e-14569.34Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GD681.863e-14468.00Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GV227.226e-14467.67Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FNU47.801e-14467.67Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Calbindin-32 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scgn1.035e-6943.54secretagogin, EF-hand calcium binding protein [Sou... [more]
calb2a8.490e-6944.12calretinin [Source:NCBI gene;Acc:103033342][more]
calb2b3.450e-6146.10calretinin-like [Source:NCBI gene;Acc:103034108][more]
calb2b1.280e-6046.10calretinin-like [Source:NCBI gene;Acc:103034108][more]
calb12.661e-5740.67calbindin 1 [Source:NCBI gene;Acc:103033937][more]
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BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scgn4.475e-6443.02secretagogin, EF-hand calcium binding protein [Sou... [more]
CALB22.704e-2642.47calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
ENSPMAT00000000059.13.118e-2150.00pep scaffold:Pmarinus_7.0:GL485405:3181:11335:1 ge... [more]
ENSPMAT00000001806.11.200e-1156.25pep scaffold:Pmarinus_7.0:GL476330:305753:307416:1... [more]
CALML32.100e-724.42calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145... [more]
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BLAST of Calbindin-32 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
CMD15.958e-629.03Calmodulin; Ca2+ binding protein that regulates Ca... [more]
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BLAST of Calbindin-32 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO459672.083e-4635.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459654.981e-3545.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459666.328e-3533.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460162.071e-1527.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459351.244e-926.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calbindin-32 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000016338.21.153e-6843.87calretinin [Source:NCBI gene;Acc:101174993][more]
calb2b1.000e-6642.86calretinin [Source:NCBI gene;Acc:101167606][more]
ENSORLT00000006100.25.216e-6340.96secretagogin [Source:NCBI gene;Acc:101157482][more]
calb2b1.677e-6242.12calretinin [Source:NCBI gene;Acc:101167606][more]
SCGN1.733e-5339.02secretagogin [Source:NCBI gene;Acc:101170527][more]
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BLAST of Calbindin-32 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000055605.10.000e+099.67SMESG000055605.1[more]
SMESG000023194.13.767e-12861.67SMESG000023194.1[more]
SMESG000003798.14.867e-10851.85SMESG000003798.1[more]
SMESG000043906.11.894e-7759.09SMESG000043906.1[more]
SMESG000043906.12.927e-7657.42SMESG000043906.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018274 ID=SMED30018274|Name=Calbindin-32|organism=Schmidtea mediterranea sexual|type=transcript|length=1131bp
ATTGGCTAGTCAGCCATCCAATAAAATCGTACTAAATTTATCATATTATA
AATGCTATTGTTTTCATTTTAAATAATTGTCTGAAAATGTCTACGACTAA
ATTCACTAATTTCCTTGCCCAATATAAAGATAATGAAACTAAGAAATTAA
GAAATTTCACCGCCACCCAATTTGCTGAGGTTTGGAGTCACTACGATGCG
GATGGCAATGGTTATATTGAGGGTTCCGAGCTGGATAGTTTCCTTAGCGA
GTTTATTGTGAGTATTCTCGGAGAAGATATAAATCCAGATGAATTAAACA
AAGAGACTTTATCCGATTTAAAAAGAGACTTCTTAGATGCTTTTGATCAA
AATTCTGACAATAAAATTGAAATGTCTGAGCTTGCCCAAGTTCTTCCCAC
TGATGAAGTATTTCTATTGTTATTTAGAAAGGATGCTCCTCTAGATTCAA
GCGTAGAATTCATGAAAATTTGGAAACAATTCGACAAAGATCAAAGCGGT
TTTATTGAAGCAGACGAACTTAAAAATTTCTTGTCTGAATTTCTGAAAGA
AGCGCGTCCTGAAGAACAAATTTCAGAAGAAAAGTTAATAGAATATACAA
GTACCATGCTACAACTATTTGACTCAAACAAAGATGGAAAGCTTCAGCTC
AGTGAAATGGCACGATTGTTACCAGTAAAGGAAAACTATCTCTGCCGACC
GATTTTCAAGAATGCCGACAAAATAACAACAGTTGATATTGAAAATGTCT
TCCATTTATATGATAGAGATAAAAATGGAACCATAGAAAATGAAGAACTT
CAAGGATTTTTGAAAGATCTCTTGGCTTTGGTGCAAGAAGATTACGACAA
AGATGATCTGGAATTCTTTCGGCAGATTATTCTCAATGAATGGGACGTCA
ACCATGACGGGAAAATTAGCATGAACGAGCTGAAAATGATGCTGATGCAA
CAAAGTCGACTGGCTAAACTTACTCACTTGGAACCCCGACAATGAGATTA
ACTGATAAAAACGGCGAAATTTGTGCCATTTCTTTTAATGTTGTCTTTAA
TCGTATTGGAATATTTTTTTATTCTTCAAAATGTTTAGTTTATTTATTGA
TAATATAGTTATATTTACCTTTGAAAAAAAA
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protein sequence of SMED30018274-orf-1

>SMED30018274-orf-1 ID=SMED30018274-orf-1|Name=SMED30018274-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=303bp
MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELD
SFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELA
QVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLS
EFLKEARPEEQISEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKEN
YLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ
EDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRLAKLTHLEP
RQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000099neuron
PLANA:0000464GABAergic neuron
PLANA:0002032epidermal cell
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 170..198
e-value: 9.6
score: 9.8
coord: 28..56
e-value: 0.0012
score: 28.1
coord: 260..289
e-value: 1.5
score: 16.3
coord: 217..245
e-value: 2.8E-4
score: 30.2
coord: 78..106
e-value: 33.0
score: 5.5
coord: 124..152
e-value: 2.6E-5
score: 33.7
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 28..101
e-value: 3.6E-8
score: 33.7
coord: 126..193
e-value: 3.0E-9
score: 37.2
coord: 217..283
e-value: 6.1E-9
score: 36.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 24..59
score: 10.915
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 73..109
score: 7.679
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 166..201
score: 8.404
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 120..155
score: 14.151
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 256..292
score: 10.245
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 213..248
score: 13.286
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 97..194
e-value: 4.0E-25
score: 89.7
coord: 195..288
e-value: 2.9E-20
score: 74.1
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 13..92
e-value: 6.6E-18
score: 66.6
NoneNo IPR availablePANTHERPTHR19972CALBINDINcoord: 12..291
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 37..49
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 179..191
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 133..145
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 87..99
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 226..238
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 270..282
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 124..288
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 29..160