SMED30018262

Overview
NameSMED30018262
Smed IDSMED30018262
Length (bp)3468
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018262 (SMED30018262) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018262 (SMED30018262) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018262 (SMED30018262) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018262 (SMED30018262) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018262

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30018262SMESG000077295.1 dd_Smed_v4_10888_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018262SMESG000077295.1 Contig34279newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30018262SMESG000077295.1 Contig34279uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30018262SMESG000077295.1 dd_Smed_v6_10888_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30018262SMESG000077295.1 dd_Smed_v4_10888_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018262 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000312:91468..107151 -16761
Homology
[Close]
BLAST of SMED30018262 vs. Planmine SMEST
Match: SMESG000077295.1 (SMESG000077295.1)

HSP 1 Score: 2112.81 bits (5473), Expect = 0.000e+0
Identity = 1111/1119 (99.29%), Postives = 1114/1119 (99.55%), Query Frame = 3 
Query:   57 MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVDVDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCILCFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIENAKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINARSTHDSVSELGIAXXXXXXXXXXXXINLINKPRRLPNTFLSPSYRRNINSPKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIAKFKQENRKLMDTIDCLNAKIEENSLFFTKKDKELEKIRKDMESTKKNISEKGDEIKQLENSNHKLRHDIDELNNKLKNKSFQCEESLKIEVNNAKEKNALQLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPILVCEEGESMELISQMKQAELXXXXXXXXXXXXXXXXXXXSELQASKAIPNSEELDSKLIFTKNQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILKTTAGYLKSELSIFEKNHEICQKDLSIVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQIENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQLQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSVTCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKDQTTSRKILPHKLSVGNDQPIFPCRKCGESLLGNDFDNEIQYPYNPIIRSPLIPKVNTNFKNSIASDISLANCKTKAIIDPSWINIDRNRTEEKFFYNNSFNHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPIETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPLNLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCETFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSSISMKFEIPNSPRRKGRIGP 3413
            MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVDVDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCILCFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIENAKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINARSTHDSVSELGIADHSKDSSKRKHKINLINKPRRLPNTFLSPSYRRNINSPKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIAKFKQENRKLMDTID LNAKIEENSLFFTKKDKELEKIRKDMESTKKNISEKGDEIKQLENSN+KLRHDIDELNNKLKNKSFQCEESLKIEVNNAKEKNALQLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPI VCEEGESMELISQMKQAELIEKFESEKKSMEEMILKLKSELQASKAIPNSEE DSKLIFT+NQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERKEIKNNNIDNLLDIWKDLLSEFELS EDKKKILKTTAGYLKSELSIFEKNHEICQKDLSIVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQIENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQLQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSVTCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKDQTTSRKILPHKLSVGNDQPIFPCRKCGESLLG+DFDNEIQYPYNPIIRSPLIPKVNTNFKNSIASDISLA CKTKAIIDPSWINIDRNRTEEKFFYNNSFNHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPIETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPLNLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCETFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSSISMKFEIPNSPRRKGRIGP
Sbjct:    1 MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVDVDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCILCFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIENAKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINARSTHDSVSELGIADHSKDSSKRKHKINLINKPRRLPNTFLSPSYRRNINSPKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIAKFKQENRKLMDTIDFLNAKIEENSLFFTKKDKELEKIRKDMESTKKNISEKGDEIKQLENSNYKLRHDIDELNNKLKNKSFQCEESLKIEVNNAKEKNALQLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPIPVCEEGESMELISQMKQAELIEKFESEKKSMEEMILKLKSELQASKAIPNSEEFDSKLIFTRNQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERKEIKNNNIDNLLDIWKDLLSEFELSFEDKKKILKTTAGYLKSELSIFEKNHEICQKDLSIVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQIENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQLQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSVTCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKDQTTSRKILPHKLSVGNDQPIFPCRKCGESLLGSDFDNEIQYPYNPIIRSPLIPKVNTNFKNSIASDISLAKCKTKAIIDPSWINIDRNRTEEKFFYNNSFNHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPIETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPLNLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCETFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSSISMKFEIPNSPRRKGRIGP 1119
[Close]
BLAST of SMED30018262 vs. Planmine SMEST
Match: SMESG000077295.1 (SMESG000077295.1)

HSP 1 Score: 1397.49 bits (3616), Expect = 0.000e+0
Identity = 738/745 (99.06%), Postives = 741/745 (99.46%), Query Frame = 3 
Query: 1179 KLKNKSFQCEESLKIEVNNAKEKNALQLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPILVCEEGESMELISQMKQAELXXXXXXXXXXXXXXXXXXXSELQASKAIPNSEELDSKLIFTKNQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXILKTTAGYLKSELSIFEKNHEICQKDLSIVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQIENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQLQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSVTCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKDQTTSRKILPHKLSVGNDQPIFPCRKCGESLLGNDFDNEIQYPYNPIIRSPLIPKVNTNFKNSIASDISLANCKTKAIIDPSWINIDRNRTEEKFFYNNSFNHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPIETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPLNLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCETFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSSISMKFEIPNSPRRKGRIGP 3413
            +LKNKSFQCEESLKIEVNNAKEKNALQLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPI VCEEGESMELISQMKQAELIEKFESEKKSMEEMILKLKSELQASKAIPNSEE DSKLIFT+NQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERKEIKNNNIDNLLDIWKDLLSEFELS EDKKKILKTTAGYLKSELSIFEKNHEICQKDLSIVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQIENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQLQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSVTCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKDQTTSRKILPHKLSVGNDQPIFPCRKCGESLLG+DFDNEIQYPYNPIIRSPLIPKVNTNFKNSIASDISLA CKTKAIIDPSWINIDRNRTEEKFFYNNSFNHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPIETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPLNLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCETFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSSISMKFEIPNSPRRKGRIGP
Sbjct:    5 QLKNKSFQCEESLKIEVNNAKEKNALQLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPIPVCEEGESMELISQMKQAELIEKFESEKKSMEEMILKLKSELQASKAIPNSEEFDSKLIFTRNQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERKEIKNNNIDNLLDIWKDLLSEFELSFEDKKKILKTTAGYLKSELSIFEKNHEICQKDLSIVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQIENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQLQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSVTCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKDQTTSRKILPHKLSVGNDQPIFPCRKCGESLLGSDFDNEIQYPYNPIIRSPLIPKVNTNFKNSIASDISLAKCKTKAIIDPSWINIDRNRTEEKFFYNNSFNHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPIETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPLNLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCETFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSSISMKFEIPNSPRRKGRIGP 749
[Close]
BLAST of SMED30018262 vs. Planmine SMEST
Match: SMESG000077295.1 (SMESG000077295.1)

HSP 1 Score: 721.465 bits (1861), Expect = 0.000e+0
Identity = 373/376 (99.20%), Postives = 375/376 (99.73%), Query Frame = 3 
Query:   57 MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVDVDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCILCFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIENAKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINARSTHDSVSELGIAXXXXXXXXXXXXINLINKPRRLPNTFLSPSYRRNINSPKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIAKFKQENRKLMDTIDCLNAKIEENSLFFTKKDKELEKIRKDMESTKKNISEKGDEIKQLENSNHKLRHDIDELNNKL 1184
            MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVDVDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCILCFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIENAKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINARSTHDSVSELGIADHSKDSSKRKHKINLINKPRRLPNTFLSPSYRRNINSPKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIAKFKQENRKLMDTID LNAKIEENSLFFTKKDKELEKIRKDMESTKKNISEKGDEIKQLENSN+KLRHDIDELNNK+
Sbjct:    1 MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVDVDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCILCFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIENAKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINARSTHDSVSELGIADHSKDSSKRKHKINLINKPRRLPNTFLSPSYRRNINSPKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIAKFKQENRKLMDTIDFLNAKIEENSLFFTKKDKELEKIRKDMESTKKNISEKGDEIKQLENSNYKLRHDIDELNNKV 376
The following BLAST results are available for this feature:
BLAST of SMED30018262 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018262 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000077295.10.000e+099.29SMESG000077295.1[more]
SMESG000077295.10.000e+099.06SMESG000077295.1[more]
SMESG000077295.10.000e+099.20SMESG000077295.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018262 ID=SMED30018262|Name=SMED30018262|organism=Schmidtea mediterranea sexual|type=transcript|length=3468bp
ATTGTAGTTGTCTATATAAAATATTTGATTATTATTAGATTTTAAAAGAA
ATTTTTATGAGTATTAGAAGTAACATTGAAGGATATGAGGAAATATTTTA
CTTAATAAAAAAGTGGTTTGATAAATCATTAAATGTTTCTATTAAAGTAC
CCGATGATTTAGCGGATGGTTCTCTTTATCTTGAACTCCTTAAAAAATGT
GTTGATGTTGATATTCCAAATAAAATTAACTTAAAATATGAAAAAATTGA
ATGTATTCTTAATGAATTTTATGGTTGTTCAACAAACTCAGCAGTTGATT
ATTATGAATTTTTGTATGGGGCAGGATGTGAAAGTGTTATCACCTTATGC
ATTTTATGTTTTGTCATGTATCGAAATTATAATGAAATCCCGAAATTAAT
CGATGAAGATTACAAACGTTTAGCCTATTTAACTATACCAATTAATGAAT
ATTTGAAAAATAAAATCAAATTTACTACCGATAGTTTTCATAAAGCTATT
GAAAATGCAAAAGAACGAGATTTGACAGAGCCTTCACATTCATCGTTTTC
TAAAATTGAAAGTTTAAAAACAGACAATCGTCAAAATGGATCGGATGATG
AAATATCAAATTCCTCAAATATCCGAAGTTGTCTAAGTGAATATTCAATA
AACGCCAGATCGACTCACGATTCAGTCAGTGAGCTTGGTATAGCTGATCA
TAGTAAAGACTCCTCAAAACGTAAGCACAAAATCAATCTTATCAATAAGC
CCCGTCGATTACCGAACACATTTCTCTCTCCTTCGTATCGACGAAACATT
AATTCGCCAAAATTAAATGAGTCCAGAAAATTTGAGCAGAGGCGGATGAA
GAACCTCGAAATTGAAAATTTAAATTTAAAACAAGAAATTGATAGCTTGA
ATGAAGATTTGGAAAAAGTCATACACAATGAAAGCAAGTCTAACGCAGAA
ATTGCGAAATTTAAACAAGAAAATCGTAAATTAATGGACACTATTGATTG
TTTAAATGCAAAAATAGAAGAAAACTCTTTATTTTTTACAAAAAAAGATA
AAGAACTGGAAAAAATTCGCAAAGATATGGAATCTACTAAAAAAAATATC
AGTGAAAAAGGTGATGAAATCAAGCAATTGGAAAACAGTAATCACAAACT
TCGTCATGATATTGACGAATTGAACAACAAGTTGAAGAACAAATCATTTC
AGTGTGAAGAATCATTGAAAATCGAAGTCAACAATGCCAAGGAGAAAAAT
GCTTTACAGCTCGAAAATGATCAGCTAAAGTGTGACGTTGAGGAGTTGAA
AAACCACATCAAAGAATTGCACAACTCAAAAACTGATATCCTCTGTCGTG
AACCGTTAGTTGTTTCACCCATACTGGTTTGCGAGGAGGGGGAGTCCATG
GAATTGATCAGTCAAATGAAGCAAGCTGAATTAATTGAAAAATTCGAATC
TGAAAAGAAATCAATGGAAGAAATGATACTGAAGCTTAAGTCAGAGCTTC
AAGCCTCCAAAGCAATACCAAACTCTGAAGAACTTGACTCAAAATTGATA
TTTACCAAGAATCAATTGGAATTGTATCAAACGCTATTGCGGGAAAAGGA
TGATGAAATGGCTAGATTACAGGAGGAATCTTTTCGATTGCATGAGGAAA
TACGTATCTTTAATCGGAAATCTGAAAGAAAAGAAATAAAGAACAATAAT
ATTGATAATTTGTTGGATATTTGGAAAGATTTGTTGTCAGAATTCGAATT
ATCTCTTGAAGATAAAAAAAAAATATTGAAGACAACAGCTGGATATTTGA
AATCGGAACTGTCTATCTTTGAAAAAAACCACGAGATTTGCCAGAAAGAT
CTCTCGATCGTATATCAGAAATTGAAAATGAATAAAGAGGAGATTAGACA
GAAAGAAGAACTTTTAGAACAAACACACAATCAGCTATTTGAAAACGAGC
AAGAAATGAATATTTTAAAGAAAGAATTATCCGACTGTCGACTGGAATAT
GAGCAAATAGAAAACATGTATAAAATCCAGAAATCCAAGAGTGCTGATGT
TGAATTCAAATTACGTCAGACATTGTTTATGGTTGACAAAGAACGGAAAG
AGATCATCTTGCAAAACACGAGCCTGAAAACAGAGATGTCGATAAGAGAA
AATCAACTTCAAAAATCCAATCAAGAATTCAACATTATGCGCAATCGATA
CGATACATGTTTGGAAGCCATTAAGACCATGACGAACGACTGTGCGGATT
ACGAAAGAGTGATTAGTGAAGTCATAGATCGACAGAGTAGGCCTTTACGC
AGCGTTACATGTCAAACCGACAGCGTAACGCAATATATTGACAAATCAAT
TTTTTCACCTAATCTTGATCGATCTGATACTTGTCGATATGAGTTTGTAA
AAGAAATGCCTGCTAATCATGAGTGTTCAGAAGTTGTCAGAGTAAATCGA
AAAGATCAGACAACTTCACGAAAGATCTTGCCCCATAAATTATCTGTTGG
TAATGACCAACCAATATTTCCCTGTCGAAAGTGCGGTGAAAGCTTGCTCG
GAAACGATTTTGATAATGAAATACAATATCCTTACAATCCCATAATCAGA
AGTCCTTTAATCCCCAAAGTGAATACCAATTTCAAAAATTCTATTGCGAG
TGATATTAGCCTTGCTAATTGCAAAACCAAAGCTATTATAGATCCGAGTT
GGATAAATATCGACAGAAATCGAACCGAGGAGAAATTCTTTTATAATAAC
TCTTTTAACCACTCGAAAGTCGCCGCCTTTCATAATAATAAATCCGTAGT
TGATGATAAAAGAGTTTATTCTCCTTCCGTTAAACCTGCAAGGATTCATC
AAATGATATCGAGGAATCTCCAGCAGCCAGACCATTTGAAAAGTAGTTAT
CCAATAGAAACTCAAACTCTCGTGCAAGGCTATTTGGCGGATTGTTTAAT
GAAAGAAATCAACTATGACTGTGCCGATGGATTTGAAACATCCGATCGGC
CAGAGCTCACTCCACGAACATCCAGTGCTCGGCATTACATTCCTGCAGAT
CCTCTGAATCTCAGGTCGACGGGCAATAACCAGAAACATTACAATTATAC
CGATGATGCATTTTCCGACCAACTGCTAAAGGGCACGGTGTCCAATGGCT
TGCAAAATCTCGTTCTCAGCGCACGCTCTAATAACCATTCCACTCCAAGA
TGCGAAACATTCAAAATTCCAATGGCGGTACCGTTGAGGAATATCTCAAA
CGATCTGAAACAGCAGACAAATCGAGATAGCGACCGGGAGAATATTATTC
CAGACGAGGAGAATAATCGTGAAAATTTGAATGACAAAATCCTATCTCAG
TCGTCAATATCAATGAAGTTTGAAATCCCTAATAGTCCCCGGAGAAAAGG
CCGTATTGGTCCTCGAAGCTCTCATAATAAAAATTCGAGAAATTGAAACC
AAATCCCAGAGATCGGAA
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protein sequence of SMED30018262-orf-1

>SMED30018262-orf-1 ID=SMED30018262-orf-1|Name=SMED30018262-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1130bp
MSIRSNIEGYEEIFYLIKKWFDKSLNVSIKVPDDLADGSLYLELLKKCVD
VDIPNKINLKYEKIECILNEFYGCSTNSAVDYYEFLYGAGCESVITLCIL
CFVMYRNYNEIPKLIDEDYKRLAYLTIPINEYLKNKIKFTTDSFHKAIEN
AKERDLTEPSHSSFSKIESLKTDNRQNGSDDEISNSSNIRSCLSEYSINA
RSTHDSVSELGIADHSKDSSKRKHKINLINKPRRLPNTFLSPSYRRNINS
PKLNESRKFEQRRMKNLEIENLNLKQEIDSLNEDLEKVIHNESKSNAEIA
KFKQENRKLMDTIDCLNAKIEENSLFFTKKDKELEKIRKDMESTKKNISE
KGDEIKQLENSNHKLRHDIDELNNKLKNKSFQCEESLKIEVNNAKEKNAL
QLENDQLKCDVEELKNHIKELHNSKTDILCREPLVVSPILVCEEGESMEL
ISQMKQAELIEKFESEKKSMEEMILKLKSELQASKAIPNSEELDSKLIFT
KNQLELYQTLLREKDDEMARLQEESFRLHEEIRIFNRKSERKEIKNNNID
NLLDIWKDLLSEFELSLEDKKKILKTTAGYLKSELSIFEKNHEICQKDLS
IVYQKLKMNKEEIRQKEELLEQTHNQLFENEQEMNILKKELSDCRLEYEQ
IENMYKIQKSKSADVEFKLRQTLFMVDKERKEIILQNTSLKTEMSIRENQ
LQKSNQEFNIMRNRYDTCLEAIKTMTNDCADYERVISEVIDRQSRPLRSV
TCQTDSVTQYIDKSIFSPNLDRSDTCRYEFVKEMPANHECSEVVRVNRKD
QTTSRKILPHKLSVGNDQPIFPCRKCGESLLGNDFDNEIQYPYNPIIRSP
LIPKVNTNFKNSIASDISLANCKTKAIIDPSWINIDRNRTEEKFFYNNSF
NHSKVAAFHNNKSVVDDKRVYSPSVKPARIHQMISRNLQQPDHLKSSYPI
ETQTLVQGYLADCLMKEINYDCADGFETSDRPELTPRTSSARHYIPADPL
NLRSTGNNQKHYNYTDDAFSDQLLKGTVSNGLQNLVLSARSNNHSTPRCE
TFKIPMAVPLRNISNDLKQQTNRDSDRENIIPDEENNRENLNDKILSQSS
ISMKFEIPNSPRRKGRIGPRSSHNKNSRN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000240body wall musculature
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR002491ABC_transptr_periplasmic_BD
Vocabulary: molecular function
TermDefinition
GO:0005488binding
GO:0003674molecular_function
GO:0008565protein transporter activity
GO:0042802identical protein binding
Vocabulary: cellular component
TermDefinition
GO:0000139Golgi membrane
GO:0005737cytoplasm
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0005794Golgi apparatus
GO:0005795Golgi stack
GO:0005856cytoskeleton
GO:0012507ER to Golgi transport vesicle membrane
GO:0016020membrane
GO:0030659cytoplasmic vesicle membrane
GO:0031410cytoplasmic vesicle
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006886intracellular protein transport
GO:0006888ER to Golgi vesicle-mediated transport
GO:0015031protein transport
GO:0035493SNARE complex assembly
GO:0045056transcytosis
GO:0048211Golgi vesicle docking
GO:0048280vesicle fusion with Golgi apparatus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 257..291
NoneNo IPR availableCOILSCoilCoilcoord: 299..319
NoneNo IPR availableCOILSCoilCoilcoord: 460..480
NoneNo IPR availableCOILSCoilCoilcoord: 504..531
NoneNo IPR availableCOILSCoilCoilcoord: 327..375
NoneNo IPR availableCOILSCoilCoilcoord: 603..654
NoneNo IPR availableCOILSCoilCoilcoord: 397..424
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1063..1129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1070..1091
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1092..1106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..185