SMED30018185

Overview
NameSMED30018185
Smed IDSMED30018185
Length (bp)2012
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30018185 (SMED30018185) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30018185 (SMED30018185) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30018185 (SMED30018185) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30018185 (SMED30018185) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018185

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30018185SMESG000003780.1 SmedASXL_015641SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30018185SMESG000003780.1 dd_Smed_v6_9295_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30018185SMESG000003780.1 dd_Smed_v4_9295_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018185 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001594:44677..50899 +9667
Homology
[Close]
BLAST of SMED30018185 vs. TrEMBL
Match: A0A1I8H040 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.016e-10
Identity = 97/392 (24.74%), Postives = 189/392 (48.21%), Query Frame = 3 
Query:  747 EPYEDMKRHLQLYKQREAELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDXXXXXXXXXXXXXXXXXXXXRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQ--ANKADRAQLNNQFKLVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKE----IIAH--LSRQNDLM-KTVELLTIQLQ 1895
            E    +K+ L+  +QRE EL +QL  +I T+E+AKLEE  A +   Q+ ++   ++ R  E++ A  +++        +    ++  L+  + ++ D+L    A    L  +  S  ++ E  R+ L   + E     D+L +  T+A L RD A+++  +++Q ++++R +   E+  +Q ++ E+R RLS++E       ++++ L    + +  EL QL +  A  A RA      K    +   R + L  +LA +E  +EA   + + ++ Q   L  +L+RE   +   L G       E   + A  +Q AE L+  +++ RD ++ L  +      ++D  R L  ++ +Q +     +  H  L R+ DL+ K  + L  Q+Q
Sbjct:  316 ESVTSLKQKLEESRQREQELYDQLTASIETVEQAKLEESNALLEKRQLSDELIGLRGRLDELLAAQSRRLDEARTGAREQADSELEGLRKRLRESEDELAAAGAKLERLNGEKRSLGQQLEEARSMLRLHETELTRGRDELASAATRAALHRDQAVSELGRVRQQLEQERVDRTGERDRLQSQLAEMRARLSAAEQAVQQGGEERLRLAGRAESLMREL-QLARSGAESAGRAARQEAEK-AAGRAARRESELLRQLAEQEARHEAAAGEMQRLLDQHAGLAERLRRELRRSADQLAGLQAAACQETAELAAERDQAAERLERCQARLRDAEKQLAGHTKAEAELRDRVRQLAGQQQDQSRGALQLLAGHTALQRERDLLAKEADFLRQQMQ 705
[Close]
BLAST of SMED30018185 vs. TrEMBL
Match: A0A267ERY3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000998g10 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.361e-10
Identity = 97/392 (24.74%), Postives = 191/392 (48.72%), Query Frame = 3 
Query:  747 EPYEDMKRHLQLYKQREAELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDXXXXXXXXXXXXXXXXXXXXRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQ--ANKADRAQLNNQFKLVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKE----IIAH--LSRQNDLM-KTVELLTIQLQ 1895
            E    +K+ L+  +QRE EL +QL  +I T+E+AKLEE  A +   Q+ ++   ++ R  E++ A  +++        +    ++  L+  + ++ D+L    A    L  +  S  ++ E  R+ L   + E     D+L +  T+A L RD A+++  +++Q ++++R +   E+  +Q ++ E+R RLS++E       ++++ L    + + +EL QL +  A  A RA      K    +   R + L  +LA +E  +EA   + + ++ Q   L  +L+RE   +   L G       E   + A  +Q AE L+  +++ RD ++ L  +      ++D  R L  ++ +Q ++    +  H  L R+ DL+ K  + L  Q+Q
Sbjct:  143 ESVTSLKQKLEESRQREQELYDQLTASIETVEQAKLEESNALLEKRQLSDELIGLRGRLDELLAAQSRRLDEARTGAREQADSELEGLRKRLRESEDELAAAGAKLERLNGEKRSLGQQLEEARSMLRLHETELTRGRDELASAATRAALHRDQAVSELGRVRQQLEQERVDRTGERDRLQSQLAEMRARLSAAEQAVQQGGEERLRLAGRAESLMSEL-QLARSGAESAGRAARQEAEK-AAGRAARRESELLRQLAEQEARHEAAAGEMQRLLDQHAGLAERLRRELRRSADQLAGLQAAACQETAELAAERDQAAERLERCQARLRDAEKQLAGHTKAEAELRDRVRQLAGQQQDQSRDALQLLAGHTALQRERDLLAKEADFLRQQMQ 532
[Close]
BLAST of SMED30018185 vs. TrEMBL
Match: A0A1S3KC34 (serologically defined colon cancer antigen 8 homolog isoform X2 OS=Lingula unguis OX=7574 GN=LOC106180680 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.377e-10
Identity = 83/394 (21.07%), Postives = 200/394 (50.76%), Query Frame = 3 
Query:  762 MKRHLQLYKQREAELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDXXXXXXXXXXXXXXXXXXXXRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMK--TELIQLVQAN--KADRAQLNNQFKLVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKEIIAHLSRQNDLMKTVELLTIQLQKIRARF--PDCC 1925
             K  L+  KQRE E   QL  +   +E+A+ ++MQA  + EQ+ +     + R +++V ++QK +  +     +  +++I +L   + + TD           +  +  + Q E E  R Q+T    +  + +++L + + +A+++R+ A+ +  +++  +D+  R++  EK+ ++E + ++R RL  +E + +N  ++ + L      ++   +L ++ + +  K  R +L+     V K+ ++R   L + +   E  +    ++ E +++QQ  LI KL+ E       L+  A ++R E+  +    E+ +  L+ + ++ ++++     +G   + M+     ++E    Q ++I+  L++Q+ LM+  +LL  +++ +RA+   P+ C
Sbjct:  393 FKSKLEDQKQREEEAYAQLKKSAELVEQAQFDKMQAITQREQLADKLHKFERRMEDLVHSHQKNVDAERELARKESKKEIEELNLRLKELTDLHGTVQHQLDRVTREKVTMQAELERTRTQVTEFDTDYTKATEELKSQLAQANIERNAAVKEMNRIRTEVDKTNRDNEMEKSRLKENLEDVRRRLMQAERELMNAKEECITLATDMQAIEKDAQLCKMAKESMEKGRREELHT----VTKRAQQREVELNSLIEEIEARHALTNAETEDMLQQQNGLIGKLREECRTLASQLEQTAQKYRGEVRQVKNLNEELSVRLEKTLNRLKEMEAQHVQHGQMHDKMRQRLAQMDEHAQYQGQQILDLLAKQSSLMRDRQLLGKEVEFLRAQVAPPNEC 782
[Close]
BLAST of SMED30018185 vs. TrEMBL
Match: A0A1S3KCH1 (serologically defined colon cancer antigen 8 homolog isoform X1 OS=Lingula unguis OX=7574 GN=LOC106180680 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.338e-10
Identity = 83/394 (21.07%), Postives = 200/394 (50.76%), Query Frame = 3 
Query:  762 MKRHLQLYKQREAELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDXXXXXXXXXXXXXXXXXXXXRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMK--TELIQLVQAN--KADRAQLNNQFKLVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKEIIAHLSRQNDLMKTVELLTIQLQKIRARF--PDCC 1925
             K  L+  KQRE E   QL  +   +E+A+ ++MQA  + EQ+ +     + R +++V ++QK +  +     +  +++I +L   + + TD           +  +  + Q E E  R Q+T    +  + +++L + + +A+++R+ A+ +  +++  +D+  R++  EK+ ++E + ++R RL  +E + +N  ++ + L      ++   +L ++ + +  K  R +L+     V K+ ++R   L + +   E  +    ++ E +++QQ  LI KL+ E       L+  A ++R E+  +    E+ +  L+ + ++ ++++     +G   + M+     ++E    Q ++I+  L++Q+ LM+  +LL  +++ +RA+   P+ C
Sbjct:  394 FKSKLEDQKQREEEAYAQLKKSAELVEQAQFDKMQAITQREQLADKLHKFERRMEDLVHSHQKNVDAERELARKESKKEIEELNLRLKELTDLHGTVQHQLDRVTREKVTMQAELERTRTQVTEFDTDYTKATEELKSQLAQANIERNAAVKEMNRIRTEVDKTNRDNEMEKSRLKENLEDVRRRLMQAERELMNAKEECITLATDMQAIEKDAQLCKMAKESMEKGRREELHT----VTKRAQQREVELNSLIEEIEARHALTNAETEDMLQQQNGLIGKLREECRTLASQLEQTAQKYRGEVRQVKNLNEELSVRLEKTLNRLKEMEAQHVQHGQMHDKMRQRLAQMDEHAQYQGQQILDLLAKQSSLMRDRQLLGKEVEFLRAQVAPPNEC 783
[Close]
BLAST of SMED30018185 vs. TrEMBL
Match: A0A267F844 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000998g4 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.536e-10
Identity = 97/392 (24.74%), Postives = 189/392 (48.21%), Query Frame = 3 
Query:  747 EPYEDMKRHLQLYKQREAELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDXXXXXXXXXXXXXXXXXXXXRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQ--ANKADRAQLNNQFKLVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKE----IIAH--LSRQNDLM-KTVELLTIQLQ 1895
            E    +K+ L+  +QRE EL +QL  +I T+E+AKLEE  A +   Q+ ++   ++ R  E++ A  +++        +    ++  L+  + ++ D+L    A    L  +  S  ++ E  R+ L   + E     D+L +  T+A L RD A+++  +++Q ++++R +   E+  +Q ++ E+R RLS++E       ++++ L    + +  EL QL +  A  A RA      K    +   R + L  +LA +E  +EA   + + ++ Q   L  +L+RE   +   L G       E   + A  +Q AE L+  +++ RD ++ L  +      ++D  R L  ++ +Q +     +  H  L R+ DL+ K  + L  Q+Q
Sbjct:  143 ESVTSLKQKLEESRQREQELYDQLTASIETVEQAKLEESNALLEKRQLSDELIGLRGRLDELLAAQSRRLDEARTGAREQADSELEGLRKRLRESEDELAAAGAKLERLNGEKRSLGQQLEEARSMLRLHETELTRGRDELASAATRAALHRDQAVSELGRVRQQLEQERVDRTGERDRLQSQLAEMRARLSAAEQAVQQGGEERLRLAGRAESLMREL-QLARSGAESAGRAARQEAEK-AAGRAARRESELLRQLAEQEARHEAAAGEMQRLLDQHAGLAERLRRELRRSADQLAGLQAAACQETAELAAERDQAAERLERCQARLRDAEKQLAGHTKAEAELRDRVRQLAGQQQDQSRGALQLLAGHTALQRERDLLAKEADFLRQQMQ 532
[Close]
BLAST of SMED30018185 vs. Planmine SMEST
Match: SMESG000003780.1 (SMESG000003780.1)

HSP 1 Score: 1168.3 bits (3021), Expect = 0.000e+0
Identity = 617/624 (98.88%), Postives = 619/624 (99.20%), Query Frame = 3 
Query:  111 MNKSDFNFKTKISRSLTDLQNSLDEACRLGLPPNLAVRLNDTEETYFCEPLDYNFQNDLTYKSASSNLERLIDQQQQQLRYAKYSKEYSENQKKQLVSNNCVGSHRNVYIDTLRMLAEIRTTLPRSQSSDAVNEIGESRNSIRNRSKVTSNRNTTKENPNDGKEIMAXXXXXXXXXXXXXXXXXXXVYRDVQLSARSGDFRSIESKCSDIDQEPYEDMKRHLQLYKQREAELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDXXXXXXXXXXXXXXXXXXXXRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQANKADRAQLNNQFKLVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKEIIAHLSRQNDLMKTVELLTIQLQKIRARFPDCCCGAFDSIDHNTPESAFLGT 1982
            MNKSDFNFKTKISRSLTDLQNSLDEACRLGLPPNLAVRLNDTEETYFCEPLDYNFQNDLTYKSASSNLERLIDQQQQQ RYAKYSKEY ENQKKQLVSNNCVGSHRNVYIDTLRMLAE RTTLPRSQSSDAVNEIGESRNSIRNRSKVTSNRNTTKENPNDGKEIMALKKKLMEMNEEMMQLRNELVYRDVQLSARSGDFRSIESKCSDIDQEPYEDMKRHLQLYKQRE+ELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDQLRLELASAALLADQLHSAQRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQANKADRAQLNNQFKL VK+LEERI ALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKEIIAHLSRQNDLMKTVELLTIQLQKIRARFPDCCCGAFDSIDHNTPESAFLGT
Sbjct:    1 MNKSDFNFKTKISRSLTDLQNSLDEACRLGLPPNLAVRLNDTEETYFCEPLDYNFQNDLTYKSASSNLERLIDQQQQQFRYAKYSKEYFENQKKQLVSNNCVGSHRNVYIDTLRMLAENRTTLPRSQSSDAVNEIGESRNSIRNRSKVTSNRNTTKENPNDGKEIMALKKKLMEMNEEMMQLRNELVYRDVQLSARSGDFRSIESKCSDIDQEPYEDMKRHLQLYKQRESELLNQLNDAISTIEEAKLEEMQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKLKNEVTKATDQLRLELASAALLADQLHSAQRENENLRAQLTGLQNEKCEQSDQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELRTRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQANKADRAQLNNQFKLAVKKLEERIAALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYSLNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGATLEFMKDSCRNLEEKKCNQDKEIIAHLSRQNDLMKTVELLTIQLQKIRARFPDCCCGAFDSIDHNTPESAFLGT 624
The following BLAST results are available for this feature:
BLAST of SMED30018185 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8H0401.016e-1024.74Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267ERY31.361e-1024.74Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1S3KC345.377e-1021.07serologically defined colon cancer antigen 8 homol... [more]
A0A1S3KCH16.338e-1021.07serologically defined colon cancer antigen 8 homol... [more]
A0A267F8446.536e-1024.74Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30018185 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018185 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000003780.10.000e+098.88SMESG000003780.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018185 ID=SMED30018185|Name=SMED30018185|organism=Schmidtea mediterranea sexual|type=transcript|length=2012bp
CTAATGTAAATGCTAGTGGTATTTAAATTTAAATTAATTGTTTTTGTATA
AGCTATTTTATAATTTGCTAATAATTTGAAAGAAATTATAACTTTTAATT
ATTAAAAGAAATGAATAAAAGTGATTTTAATTTTAAAACAAAAATAAGTC
GTTCGCTGACTGACTTGCAAAACTCTTTAGATGAGGCTTGTAGATTGGGA
CTACCACCAAATTTAGCTGTCCGACTGAATGATACTGAGGAAACTTATTT
CTGTGAGCCATTGGATTATAATTTCCAAAATGATTTGACATACAAATCGG
CGTCGAGTAATCTGGAAAGACTAATTGATCAGCAGCAGCAGCAGTTGAGA
TATGCGAAATATTCAAAGGAATATTCTGAAAATCAGAAGAAACAACTAGT
CAGCAACAACTGTGTTGGGAGCCACAGAAATGTATACATCGACACGCTGC
GGATGCTGGCAGAAATCCGAACAACTTTACCGAGAAGCCAATCAAGTGAT
GCGGTGAATGAAATCGGGGAAAGTCGAAATAGCATCCGAAACCGGAGTAA
AGTAACTAGTAATAGAAACACTACCAAAGAAAACCCAAACGACGGAAAAG
AAATTATGGCACTTAAGAAGAAATTAATGGAAATGAATGAGGAAATGATG
CAGTTGCGGAATGAATTGGTTTATCGAGATGTGCAGTTGTCAGCCAGAAG
CGGCGATTTCCGATCAATTGAGTCCAAATGTTCTGATATTGACCAGGAAC
CGTACGAAGATATGAAACGACATCTTCAGTTATACAAGCAGCGGGAGGCG
GAATTGCTCAATCAATTGAATGATGCAATCTCTACGATTGAGGAAGCAAA
ATTGGAAGAAATGCAGGCGAGCATGCGTGCCGAACAAATAAAGGAGGATT
TCGATAACATTAAAATCAGATTTCAAGAAGTAGTTGAGGCGAACCAGAAG
AAAATTGGAAATCAAAATGCGTTTGAAGAGCAATTCGTCCAAGAAAAGAT
AATGAAACTGAAAAATGAGGTCACTAAGGCAACAGATCAGCTAAGACTCG
AGTTGGCTTCTGCTGCTTTGTTGGCAGATCAATTGCATTCGGCACAGCGT
GAGAACGAGAATCTACGCGCTCAACTGACGGGATTGCAGAATGAGAAATG
TGAACAGAGCGATCAGTTGACGACCGTTGTTACCAAGGCGCATCTTCAAC
GAGATATGGCAATGGCGGACTCAGCTAAGCTACAGCAAATCATCGACAGG
GACAGAAGGAATCATGCCCAGGAAAAGGCAATCATGCAAGAGGAAATGAA
TGAGCTGCGAACGAGATTGAGCTCATCTGAAAATCAGCATTTGAATCTGG
CCCAGGATAAAATGGGCCTCCTCAATGCTCAGGATCGCATGAAGACGGAG
TTGATCCAGTTGGTCCAGGCGAACAAGGCGGATCGCGCCCAACTAAATAA
TCAGTTCAAGTTGGTTGTCAAGCAACTAGAGGAAAGGATAACTGCGTTGC
AAACGGAACTGGCAAGAAAAGAGAAATTGTACGAGGCGATGCTGAGTGAC
GCGGAGGGAGTAATGCGCCAGCAGATATCACTGATCAACAAACTGAAACG
GGAGTATAGCTTGAATGTGCAGGGATTGGATGGGTTTGCTGACAGGCACC
GAGATGAGCTGGATTCGATTCATGCCAGATTCGAGCAATGTGCCGAGGAG
CTCCAGGTGAGCAAGTCCCAGAATCGAGATCTGCAGCAATCACTAGACAA
TTATGGAGCCACCTTGGAATTCATGAAGGACTCTTGTCGAAATCTTGAGG
AAAAGAAGTGCAATCAAGACAAAGAAATCATAGCCCATTTGTCCCGGCAG
AACGACCTGATGAAAACCGTGGAACTGTTGACCATTCAGTTACAGAAAAT
CCGTGCCAGGTTTCCCGACTGCTGCTGTGGTGCTTTCGATTCGATCGATC
ATAATACTCCAGAGTCTGCTTTTTTGGGAACTATTGGCTAAATATATATA
TATATATATATA
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protein sequence of SMED30018185-orf-1

>SMED30018185-orf-1 ID=SMED30018185-orf-1|Name=SMED30018185-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=627bp
MNKSDFNFKTKISRSLTDLQNSLDEACRLGLPPNLAVRLNDTEETYFCEP
LDYNFQNDLTYKSASSNLERLIDQQQQQLRYAKYSKEYSENQKKQLVSNN
CVGSHRNVYIDTLRMLAEIRTTLPRSQSSDAVNEIGESRNSIRNRSKVTS
NRNTTKENPNDGKEIMALKKKLMEMNEEMMQLRNELVYRDVQLSARSGDF
RSIESKCSDIDQEPYEDMKRHLQLYKQREAELLNQLNDAISTIEEAKLEE
MQASMRAEQIKEDFDNIKIRFQEVVEANQKKIGNQNAFEEQFVQEKIMKL
KNEVTKATDQLRLELASAALLADQLHSAQRENENLRAQLTGLQNEKCEQS
DQLTTVVTKAHLQRDMAMADSAKLQQIIDRDRRNHAQEKAIMQEEMNELR
TRLSSSENQHLNLAQDKMGLLNAQDRMKTELIQLVQANKADRAQLNNQFK
LVVKQLEERITALQTELARKEKLYEAMLSDAEGVMRQQISLINKLKREYS
LNVQGLDGFADRHRDELDSIHARFEQCAEELQVSKSQNRDLQQSLDNYGA
TLEFMKDSCRNLEEKKCNQDKEIIAHLSRQNDLMKTVELLTIQLQKIRAR
FPDCCCGAFDSIDHNTPESAFLGTIG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR031887SDCCAG8
Vocabulary: biological process
TermDefinition
GO:0007098centrosome cycle
GO:0051297centrosome organization
Vocabulary: cellular component
TermDefinition
GO:0005813centrosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 318..345
NoneNo IPR availableCOILSCoilCoilcoord: 446..480
NoneNo IPR availableCOILSCoilCoilcoord: 382..416
NoneNo IPR availableCOILSCoilCoilcoord: 215..263
NoneNo IPR availableCOILSCoilCoilcoord: 545..565
NoneNo IPR availableCOILSCoilCoilcoord: 290..310
NoneNo IPR availableCOILSCoilCoilcoord: 165..185
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..160
IPR031887Serologically defined colon cancer antigen 8PANTHERPTHR34343FAMILY NOT NAMEDcoord: 217..598