Bestrophin homolog

Overview
NameBestrophin homolog
Smed IDSMED30018184
Length (bp)1369
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Bestrophin homolog (SMED30018184) t-SNE clustered cells

Violin plots show distribution of expression levels for Bestrophin homolog (SMED30018184) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Bestrophin homolog (SMED30018184) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Bestrophin homolog (SMED30018184) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018184

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018184SMESG000016291.1 dd_Smed_v4_1844_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Bestrophin homolog vs. Ensembl Human
Match: BEST3 (bestrophin 3 [Source:HGNC Symbol;Acc:HGNC:17105])

HSP 1 Score: 168.703 bits (426), Expect = 3.679e-47
Identity = 120/399 (30.08%), Postives = 195/399 (48.87%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAV---------------------IAR------ASDSCFPEYLNSSLE 1163
            +V Y S V+      F +LL +W+ S++KLL+ +F+ F ++YT I+L+YRL L+   QK+  FE +  Y ++ A  +P++F+LGF+V+ V+ R+W    ++PW  +  FL  + ++G +E   R LR T++RY+NL+ +L    +S  V KRF                P +        + +DE  ++ F  L              K    ++++P +W   L  KAR    I+      S++  +   ++    L  ++   +PLVYTQV+ + VYTF    LI   F   T      D++ Y PIF++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S +                      AR      A+D C P +L S+++
Sbjct:    2 TVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRL-LLTGVQKRY-FEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDE-HGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRF----------------PTMDHVVEAGFMTTDE--RKLFNHL--------------KSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQ 365
[Close]
BLAST of Bestrophin homolog vs. Ensembl Human
Match: BEST3 (bestrophin 3 [Source:HGNC Symbol;Acc:HGNC:17105])

HSP 1 Score: 167.548 bits (423), Expect = 8.006e-45
Identity = 119/399 (29.82%), Postives = 193/399 (48.37%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAV---------------------IAR------ASDSCFPEYLNSSLE 1163
            +V Y S V+      F +LL +W+ S++KLL+ +F+ F ++YT I+L+YRL L     +K  FE +  Y ++ A  +P++F+LGF+V+ V+ R+W    ++PW  +  FL  + ++G +E   R LR T++RY+NL+ +L    +S  V KRF                P +        + +DE  ++ F  L              K    ++++P +W   L  KAR    I+      S++  +   ++    L  ++   +PLVYTQV+ + VYTF    LI   F   T      D++ Y PIF++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S +                      AR      A+D C P +L S+++
Sbjct:    2 TVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTG--VQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDE-HGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRF----------------PTMDHVVEAGFMTTDE--RKLFNHL--------------KSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQ 365
[Close]
BLAST of Bestrophin homolog vs. Ensembl Human
Match: BEST1 (bestrophin 1 [Source:HGNC Symbol;Acc:HGNC:12703])

HSP 1 Score: 164.466 bits (415), Expect = 3.677e-44
Identity = 108/354 (30.51%), Postives = 180/354 (50.85%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQ--FFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            ++ Y S V+     +F +LL  W+ S++KLL+ +FL F L Y  I  +YRL L ++ Q  ++FE +  Y +    L+P+SF+LGF+V+ V+ R+W    ++PW  +   L    + G +E + R LR T+IRY NL  VL +  +S  V KRF                            ++  +V+  F   +   E   LE L    + P   F++P +W   L  KA     I+      S++N +  ++    +L  ++  ++PLVYTQV+ + VY+F ++ +  +Q +      P  +++   P+F+  +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQ--LMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDE-QGRLLRRTLIRYANLGNVLILRSVSTAVYKRF---------------------------PSAQHLVQAGF---MTPAEHKQLEKL----SLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Human
Match: BEST4 (bestrophin 4 [Source:HGNC Symbol;Acc:HGNC:17106])

HSP 1 Score: 162.155 bits (409), Expect = 4.679e-44
Identity = 109/367 (29.70%), Postives = 184/367 (50.14%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQ--------QTITNPN-------IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+      F  LL RW+ S++KLL+ +FL F  +Y  +++ YRL L +  +++ V+  +  Y N++A L+PLSF+LGF+V+ V+ R+W+   SIP   +   +  A ++G ++ + R LR T+IRY NL+ VL +  +S +V KRF   +      +++        R++   + SD      F K                     +++P +W T L  +AR+   I+ D     ++  +   +     L  ++  ++PLVYTQV+ I VY+F    L+   F +        Q +  P         D + Y P+ ++ +FF Y GW KV    INPFG DD D   ++++D NLQ+S
Sbjct:    2 TVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQ--EQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQ-RGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTME-----HVVDAGFMSQEERKKFESLKSD------FNK---------------------YWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVS 333
[Close]
BLAST of Bestrophin homolog vs. Ensembl Human
Match: BEST2 (bestrophin 2 [Source:HGNC Symbol;Acc:HGNC:17107])

HSP 1 Score: 154.066 bits (388), Expect = 6.796e-41
Identity = 102/352 (28.98%), Postives = 178/352 (50.57%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNP----NIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F +LL  W+ S++KLLW + L F   Y  ++  YR  L +   +K  FE +V Y ++ A+L+P+SF+LGF+V+ V+ R+W+    +P       +    ++G ++ + R  R T++RY  LS VL +  +S  V KRF                P I           D +V+  F   +  EE    E L+   +  ++++P +W + L  +AR+   I+ +S    ++  +   +     L  ++  +VPLVYTQV+ I +Y++ ++ +  +Q +       + D++   PIF++ +FF Y GW KV    INPFG DD D   + ++D N Q+S
Sbjct:    2 TVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGFYMALSAAYRFVLTEG--QKRYFEKLVIYCDQYASLIPVSFVLGFYVTLVVNRWWSQYLCMPLPDALMCVVAGTVHGRDD-RGRLYRRTLMRYAGLSAVLILRSVSTAVFKRF----------------PTI-----------DHVVEAGF---MTREERKKFENLNS--SYNKYWVPCVWFSNLAAQARREGRIRDNSALKLLLEELNVFRGKCGMLFHYDWISVPLVYTQVVTIALYSYFLACLIGRQFLDPAQGYKDHDLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Celegans
Match: best-22 (Bestrophin homolog 22 [Source:UniProtKB/Swiss-Prot;Acc:Q23369])

HSP 1 Score: 154.451 bits (389), Expect = 1.259e-41
Identity = 109/348 (31.32%), Postives = 179/348 (51.44%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYM-NKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYT-FLISEIFSQQTITNPN-IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNL 1094
            ++ Y   VS     +F  LL RW+ SVWK ++     +  ++  I+ +YR  L   P ++ VFE ++ Y  NK  A +PL+FLLGFFVS V+ R+ +I+N I W+   + L    + G +E ++R +R  ++RYL LS  L +  +S +VRKRF       + + L  +                         L+  EEM +L+ + D  +  +++  + W+  LV + +K   + S    + +V+ I   ++ L +L  ++   VPLVY QVI + V   F+I  I  Q  +T PN   I+ + PI ++ +F +YMGW KV    +NP G DD DL  + ++D NL
Sbjct:    2 TISYTLDVSQTNLQSFFSLLLRWRGSVWKAVFGQLAVWTAVFLLISCIYRYML--SPSQQDVFEQLIRYFDNKLDANIPLTFLLGFFVSFVVARWGSILNGIGWIDDASLLFATYIRGADE-ETRVIRRNLVRYLVLSQALVLRDISMQVRKRF------PTMDTLAAS------------------------GLMTHEEMDILDHIKDPYS--RYWTSIQWSLNLVYECQKKGKVDSYYLMNKIVDEIGKFRHGLASLLKYDWVPVPLVYPQVIFLAVRIYFMICLIGRQFIVTGPNPSGIDLWLPITTMVQFLVYMGWMKVAEALLNPLGEDDDDLECNYIIDKNL 314
[Close]
BLAST of Bestrophin homolog vs. Ensembl Celegans
Match: best-22 (Bestrophin homolog 22 [Source:UniProtKB/Swiss-Prot;Acc:Q23369])

HSP 1 Score: 154.451 bits (389), Expect = 1.424e-41
Identity = 110/348 (31.61%), Postives = 179/348 (51.44%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYM-NKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYT-FLISEIFSQQTITNPN-IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNL 1094
            ++ Y   VS     +F  LL RW+ SVWK ++     +  ++  I+ +YR  L   P ++ VFE ++ Y  NK  A +PL+FLLGFFVS V+ R+ +I+N I W+   + L    + G +E ++R +R  ++RYL LS  L +  +S +VRKRF                P +           D +       L+  EEM +L+ + D  +  +++  + W+  LV + +K   + S    + +V+ I   ++ L +L  ++   VPLVY QVI + V   F+I  I  Q  +T PN   I+ + PI ++ +F +YMGW KV    +NP G DD DL  + ++D NL
Sbjct:    2 TISYTLDVSQTNLQSFFSLLLRWRGSVWKAVFGQLAVWTAVFLLISCIYRYML--SPSQQDVFEQLIRYFDNKLDANIPLTFLLGFFVSFVVARWGSILNGIGWIDDASLLFATYIRGADE-ETRVIRRNLVRYLVLSQALVLRDISMQVRKRF----------------PTM-----------DTLAA---SGLMTHEEMDILDHIKDPYS--RYWTSIQWSLNLVYECQKKGKVDSYYLMNKIVDEIGKFRHGLASLLKYDWVPVPLVYPQVIFLAVRIYFMICLIGRQFIVTGPNPSGIDLWLPITTMVQFLVYMGWMKVAEALLNPLGEDDDDLECNYIIDKNL 314
[Close]
BLAST of Bestrophin homolog vs. Ensembl Celegans
Match: best-22 (Bestrophin homolog 22 [Source:UniProtKB/Swiss-Prot;Acc:Q23369])

HSP 1 Score: 154.451 bits (389), Expect = 1.575e-41
Identity = 109/348 (31.32%), Postives = 176/348 (50.57%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYM-NKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYT-FLISEIFSQQTITNPN-IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNL 1094
            ++ Y   VS     +F  LL RW+ SVWK ++     +  ++  I+ +YR  L   P ++ VFE ++ Y  NK  A +PL+FLLGFFVS V+ R+ +I+N I W+   + L    + G +E ++R +R  ++RYL LS  L +  +S +VRKRF                                M       L+  EEM +L+ + D  +  +++  + W+  LV + +K   + S    + +V+ I   ++ L +L  ++   VPLVY QVI + V   F+I  I  Q  +T PN   I+ + PI ++ +F +YMGW KV    +NP G DD DL  + ++D NL
Sbjct:    2 TISYTLDVSQTNLQSFFSLLLRWRGSVWKAVFGQLAVWTAVFLLISCIYRYML--SPSQQDVFEQLIRYFDNKLDANIPLTFLLGFFVSFVVARWGSILNGIGWIDDASLLFATYIRGADE-ETRVIRRNLVRYLVLSQALVLRDISMQVRKRF------------------------------PTMDTLAASGLMTHEEMDILDHIKDPYS--RYWTSIQWSLNLVYECQKKGKVDSYYLMNKIVDEIGKFRHGLASLLKYDWVPVPLVYPQVIFLAVRIYFMICLIGRQFIVTGPNPSGIDLWLPITTMVQFLVYMGWMKVAEALLNPLGEDDDDLECNYIIDKNL 314
[Close]
BLAST of Bestrophin homolog vs. Ensembl Celegans
Match: best-22 (Bestrophin homolog 22 [Source:UniProtKB/Swiss-Prot;Acc:Q23369])

HSP 1 Score: 154.451 bits (389), Expect = 1.947e-41
Identity = 109/348 (31.32%), Postives = 176/348 (50.57%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYM-NKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYT-FLISEIFSQQTITNPN-IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNL 1094
            ++ Y   VS     +F  LL RW+ SVWK ++     +  ++  I+ +YR  L   P ++ VFE ++ Y  NK  A +PL+FLLGFFVS V+ R+ +I+N I W+   + L    + G +E ++R +R  ++RYL LS  L +  +S +VRKRF                                M       L+  EEM +L+ + D  +  +++  + W+  LV + +K   + S    + +V+ I   ++ L +L  ++   VPLVY QVI + V   F+I  I  Q  +T PN   I+ + PI ++ +F +YMGW KV    +NP G DD DL  + ++D NL
Sbjct:    2 TISYTLDVSQTNLQSFFSLLLRWRGSVWKAVFGQLAVWTAVFLLISCIYRYML--SPSQQDVFEQLIRYFDNKLDANIPLTFLLGFFVSFVVARWGSILNGIGWIDDASLLFATYIRGADE-ETRVIRRNLVRYLVLSQALVLRDISMQVRKRF------------------------------PTMDTLAASGLMTHEEMDILDHIKDPYS--RYWTSIQWSLNLVYECQKKGKVDSYYLMNKIVDEIGKFRHGLASLLKYDWVPVPLVYPQVIFLAVRIYFMICLIGRQFIVTGPNPSGIDLWLPITTMVQFLVYMGWMKVAEALLNPLGEDDDDLECNYIIDKNL 314
[Close]
BLAST of Bestrophin homolog vs. Ensembl Celegans
Match: best-22 (Bestrophin homolog 22 [Source:UniProtKB/Swiss-Prot;Acc:Q23369])

HSP 1 Score: 154.451 bits (389), Expect = 2.038e-41
Identity = 109/348 (31.32%), Postives = 179/348 (51.44%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYM-NKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYT-FLISEIFSQQTITNPN-IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNL 1094
            ++ Y   VS     +F  LL RW+ SVWK ++     +  ++  I+ +YR  L   P ++ VFE ++ Y  NK  A +PL+FLLGFFVS V+ R+ +I+N I W+   + L    + G +E ++R +R  ++RYL LS  L +  +S +VRKRF       + + L  +                         L+  EEM +L+ + D  +  +++  + W+  LV + +K   + S    + +V+ I   ++ L +L  ++   VPLVY QVI + V   F+I  I  Q  +T PN   I+ + PI ++ +F +YMGW KV    +NP G DD DL  + ++D NL
Sbjct:    2 TISYTLDVSQTNLQSFFSLLLRWRGSVWKAVFGQLAVWTAVFLLISCIYRYML--SPSQQDVFEQLIRYFDNKLDANIPLTFLLGFFVSFVVARWGSILNGIGWIDDASLLFATYIRGADE-ETRVIRRNLVRYLVLSQALVLRDISMQVRKRF------PTMDTLAAS------------------------GLMTHEEMDILDHIKDPYS--RYWTSIQWSLNLVYECQKKGKVDSYYLMNKIVDEIGKFRHGLASLLKYDWVPVPLVYPQVIFLAVRIYFMICLIGRQFIVTGPNPSGIDLWLPITTMVQFLVYMGWMKVAEALLNPLGEDDDDLECNYIIDKNL 314
[Close]
BLAST of Bestrophin homolog vs. Ensembl Fly
Match: Best4 (gene:FBgn0036491 transcript:FBtr0113172)

HSP 1 Score: 204.912 bits (520), Expect = 7.194e-60
Identity = 118/367 (32.15%), Postives = 200/367 (54.50%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVT-FLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDK-PTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ----TITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPE 1142
            +V Y S VS +  ++ FL LL RW+ S++KL+W D + FF +Y  + + YRL +  + + K  FE+I++Y  K  +L+PLSF+LGFFV  V+ R+W    +IPW    A L    ++G ++ ++R +R TI+RY+ L  V+    +S +V++RF                P  +           ++++  F   ++E E  ++E +D   P+ P+ ++P++WA  +V +AR+ + I+ D    ++++ +  ++ N   L  ++  +VPLVYTQV+ +  Y+F +  +  QQ            I  +FPI ++ +FF YMGW KV  T INPFG DD D  L+ ++D NL +S  I        PE
Sbjct:    2 TVTYTSRVSTSSVLSNFLGLLVRWRGSIYKLIWLDLIVFFGLYCLLAITYRLLV--NEEGKRFFEAIMSYCEKNGSLIPLSFVLGFFVRIVMNRWWDQYTTIPWPDGIAILISTSIHGSDD-RARVMRRTILRYVCLCQVIVFTMISPRVKRRF----------------PTYT-----------QIIEAGF---LLENEKKIIEAMDQAFPSYPKHWMPIVWAASIVMRARRENKIRDDYAVKTIIDELNQLRGNCGFLLYYDWISVPLVYTQVVTVATYSFFLFSVLGQQWNESHSDEDGTRIRRWFPILTVLQFFFYMGWLKVAETLINPFGEDDDDFELNWIIDRNLTVSYCIVDEMHQEHPE 335
[Close]
BLAST of Bestrophin homolog vs. Ensembl Fly
Match: Best1 (gene:FBgn0040238 transcript:FBtr0082183)

HSP 1 Score: 205.682 bits (522), Expect = 1.078e-58
Identity = 120/356 (33.71%), Postives = 188/356 (52.81%), Query Frame = 3 
Query:  105 FLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDK-PTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ-----TITNPNI--DIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYL 1148
            FLKLL RW+ S++KL+W D L F  IY  I ++YR  L  +P +K  FE+IV Y +    L+PLSF+LGF+VS V+ R+W    SIPW    A    + ++G +E + R +R TI+RY+ L   + +  +S +V+KRF          LLN N                              E T++E ++   P   + ++P++WA  ++ +ARK   I+ D    ++++ +   +     L  ++T +VPLVYTQV+ + VY++ ++    QQ      + N      ++ YFP+F+  +FF YMGW KV  + INPFG DD D  ++ M+D NLQ+S +I        PE L
Sbjct:   18 FLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGL--NPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDE-RGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDN------------------------------EKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELL 340
[Close]
BLAST of Bestrophin homolog vs. Ensembl Fly
Match: Best1 (gene:FBgn0040238 transcript:FBtr0337020)

HSP 1 Score: 205.297 bits (521), Expect = 1.633e-58
Identity = 120/356 (33.71%), Postives = 188/356 (52.81%), Query Frame = 3 
Query:  105 FLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDK-PTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ-----TITNPNI--DIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYL 1148
            FLKLL RW+ S++KL+W D L F  IY  I ++YR  L  +P +K  FE+IV Y +    L+PLSF+LGF+VS V+ R+W    SIPW    A    + ++G +E + R +R TI+RY+ L   + +  +S +V+KRF          LLN N                              E T++E ++   P   + ++P++WA  ++ +ARK   I+ D    ++++ +   +     L  ++T +VPLVYTQV+ + VY++ ++    QQ      + N      ++ YFP+F+  +FF YMGW KV  + INPFG DD D  ++ M+D NLQ+S +I        PE L
Sbjct:   18 FLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGL--NPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDE-RGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDN------------------------------EKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELL 340
[Close]
BLAST of Bestrophin homolog vs. Ensembl Fly
Match: Best1 (gene:FBgn0040238 transcript:FBtr0114590)

HSP 1 Score: 205.297 bits (521), Expect = 2.345e-58
Identity = 120/356 (33.71%), Postives = 188/356 (52.81%), Query Frame = 3 
Query:  105 FLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDK-PTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ-----TITNPNI--DIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYL 1148
            FLKLL RW+ S++KL+W D L F  IY  I ++YR  L  +P +K  FE+IV Y +    L+PLSF+LGF+VS V+ R+W    SIPW    A    + ++G +E + R +R TI+RY+ L   + +  +S +V+KRF          LLN N                              E T++E ++   P   + ++P++WA  ++ +ARK   I+ D    ++++ +   +     L  ++T +VPLVYTQV+ + VY++ ++    QQ      + N      ++ YFP+F+  +FF YMGW KV  + INPFG DD D  ++ M+D NLQ+S +I        PE L
Sbjct:   18 FLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGL--NPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDE-RGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDN------------------------------EKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELL 340
[Close]
BLAST of Bestrophin homolog vs. Ensembl Fly
Match: Best1 (gene:FBgn0040238 transcript:FBtr0306023)

HSP 1 Score: 205.297 bits (521), Expect = 3.215e-58
Identity = 120/356 (33.71%), Postives = 188/356 (52.81%), Query Frame = 3 
Query:  105 FLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDK-PTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ-----TITNPNI--DIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYL 1148
            FLKLL RW+ S++KL+W D L F  IY  I ++YR  L  +P +K  FE+IV Y +    L+PLSF+LGF+VS V+ R+W    SIPW    A    + ++G +E + R +R TI+RY+ L   + +  +S +V+KRF          LLN N                              E T++E ++   P   + ++P++WA  ++ +ARK   I+ D    ++++ +   +     L  ++T +VPLVYTQV+ + VY++ ++    QQ      + N      ++ YFP+F+  +FF YMGW KV  + INPFG DD D  ++ M+D NLQ+S +I        PE L
Sbjct:   18 FLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGL--NPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDE-RGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDN------------------------------EKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELL 340
[Close]
BLAST of Bestrophin homolog vs. Ensembl Zebrafish
Match: best4 (bestrophin 4 [Source:ZFIN;Acc:ZDB-GENE-070912-6])

HSP 1 Score: 177.563 bits (449), Expect = 4.372e-51
Identity = 115/352 (32.67%), Postives = 186/352 (52.84%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+  +   F KLLFRW+ S++KLL+ +FL F L+YT I++LYR  L  + Q+  +F  +  + NK A L+P+SF+LGF+V+    R+W    S P       +    ++G +E + R LR T++RY NLS VL +  +S +VRKRF   +     +++           +LN + SD      F K                     +++PL W   L  +ARK   +K D     +++ + + ++    L  ++  ++PLVYTQV+ + VY+F    LI   F +   +  ++D+  Y PIF++ +FF Y GW KVG   INPFG DD D   ++++D N+Q+S
Sbjct:    2 TVSYTLKVANAKFGGFSKLLFRWKGSIYKLLYREFLVFCLLYTFISILYRCVLNNNQQE--IFARLARHCNKFAKLIPMSFVLGFYVTQAFQRWWGQYTSFPLPDNLMMVVSGNVHGTDE-RGRLLRRTLMRYANLSSVLILRSISTRVRKRFTTLE-----DIVEAGFMTTDELRKLNHLYSD------FNK---------------------YWMPLAWFANLASQARKEGRVKDDIALRLLMDELNNYRSKCSMLFHYDWISIPLVYTQVVTVAVYSFFTFSLIGRQFVKPASSEEHLDL--YIPIFTLLEFFFYAGWLKVGELIINPFGEDDDDFETNQLIDRNIQVS 316
[Close]
BLAST of Bestrophin homolog vs. Ensembl Zebrafish
Match: best4 (bestrophin 4 [Source:ZFIN;Acc:ZDB-GENE-070912-6])

HSP 1 Score: 176.407 bits (446), Expect = 5.346e-51
Identity = 117/365 (32.05%), Postives = 192/365 (52.60%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQI-----SAVIARASD 1127
            +V Y   V+  +   F KLLFRW+ S++KLL+ +FL F L+YT I++LYR  L  + Q+  +F  +  + NK A L+P+SF+LGF+V+    R+W    S P       +    ++G +E + R LR T++RY NLS VL +  +S +VRKRF   +     +++           +LN + SD      F K                     +++PL W   L  +ARK   +K D     +++ + + ++    L  ++  ++PLVYTQV+ + VY+F    LI   F +   +  ++D+  Y PIF++ +FF Y GW KVG   INPFG DD D   ++++D N+Q+     S +I+R ++
Sbjct:    2 TVSYTLKVANAKFGGFSKLLFRWKGSIYKLLYREFLVFCLLYTFISILYRCVLNNNQQE--IFARLARHCNKFAKLIPMSFVLGFYVTQAFQRWWGQYTSFPLPDNLMMVVSGNVHGTDE-RGRLLRRTLMRYANLSSVLILRSISTRVRKRFTTLE-----DIVEAGFMTTDELRKLNHLYSD------FNK---------------------YWMPLAWFANLASQARKEGRVKDDIALRLLMDELNNYRSKCSMLFHYDWISIPLVYTQVVTVAVYSFFTFSLIGRQFVKPASSEEHLDL--YIPIFTLLEFFFYAGWLKVGELIINPFGEDDDDFETNQLIDRNIQVCRLKNSIIISRNAN 329
[Close]
BLAST of Bestrophin homolog vs. Ensembl Zebrafish
Match: best2 (bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127])

HSP 1 Score: 174.481 bits (441), Expect = 4.562e-49
Identity = 118/354 (33.33%), Postives = 183/354 (51.69%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLG--FFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F KLL  W+ S++K+L+ +FL FF +YT I++ YR +L+ D QK+  FE +  Y +  A+L+P+SF+LG  F+V+ V+ R+W+   SIP   +   +    + G +E + R LR T++RY +LS +L +  +S  V KRF                P I           D +V+  F   +  EE    E L       +++IP +W T L   AR    IK D+T   ++  + + +     L  ++  +VPLVYTQV+ + VY+F    LI   F       P  D++ Y PIF++ +FF Y GW KV    INPFG DD D   + ++D N Q+S
Sbjct:    2 TVTYTARVANARFCGFSKLLLAWKGSIYKVLYKEFLAFFAMYTAISITYRFFLV-DGQKR-YFEKLSIYCDNYASLIPMSFVLGMRFYVTLVVNRWWSQYTSIPLPDRVMCVLSGGVQGSDE-RGRLLRRTLMRYSSLSALLILRSVSTAVFKRF----------------PTI-----------DHVVEAGF---MTREERKKFESLHSPYN--KYWIPCVWFTNLAAAARCEGRIKDDNTYKLLLEELNNFRGKCSMLFHYDMISVPLVYTQVVTLAVYSFFLVCLIGRQFLDPGQGYPGHDLDLYVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNFQVS 320
[Close]
BLAST of Bestrophin homolog vs. Ensembl Zebrafish
Match: best1 (bestrophin 1 [Source:ZFIN;Acc:ZDB-GENE-110411-214])

HSP 1 Score: 176.792 bits (447), Expect = 2.352e-48
Identity = 108/352 (30.68%), Postives = 190/352 (53.98%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+     TF +LL RW+ S++KLL+ + L F ++Y  I++ YR  L +  +++ +FE +  Y ++ A L+P+SF+LGF+V+ V+ R+W    S+PW  + + L    + G +E  +R +R +++RY NLS +L    +S  V KRF                P +S            +V+     L+  EE+  L+ L       +F++P MW   L  +AR    I +D   ++++N +  +++    L  ++  ++PLVYTQV+ + VY+F ++ +  +Q +      P  +++ Y P+F++ +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSRRVADARLGTFYRLLLRWKGSIYKLLYRELLIFTVMYCIISITYRCVLTE--EQRRMFEKLSLYCDQYAQLIPVSFVLGFYVTLVVSRWWGQFESVPWPDRLSALVSGHVRGADE-GARLIRRSLMRYANLSGILIYRSVSTAVYKRF----------------PTMS-----------HLVQ---AGLMTAEELRQLQELPSPHN--KFWVPCMWFVSLAMRARSEGRINNDVAMTAILNELNTLRSQCMRLYGYDWISLPLVYTQVVTVAVYSFFLTCLIGRQFLDPAQGYPGHNLDFYLPVFTLLQFFFYVGWLKVAEQLINPFGEDDDDFETNWLVDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Zebrafish
Match: best2 (bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127])

HSP 1 Score: 109.383 bits (272), Expect = 5.233e-27
Identity = 81/260 (31.15%), Postives = 131/260 (50.38%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAV 839
            +V Y + V+      F KLL  W+ S++K+L+ +FL FF +YT I++ YR +L+ D QK+  FE +  Y +  A+L+P+SF+LGF+V+ V+ R+W+   SIP   +   +    + G +E + R LR T++RY +LS +L +  +S  V KRF                P I           D +V+  F   +  EE    E L       +++IP +W T L   AR    IK D+T   ++  +  +KN    + V
Sbjct:    2 TVTYTARVANARFCGFSKLLLAWKGSIYKVLYKEFLAFFAMYTAISITYRFFLV-DGQKR-YFEKLSIYCDNYASLIPMSFVLGFYVTLVVNRWWSQYTSIPLPDRVMCVLSGGVQGSDE-RGRLLRRTLMRYSSLSALLILRSVSTAVFKRF----------------PTI-----------DHVVEAGF---MTREERKKFESLHSPYN--KYWIPCVWFTNLAAAARCEGRIKDDNTYKLLLE-VNTLKNGFIKVRV 225
[Close]
BLAST of Bestrophin homolog vs. Ensembl Xenopus
Match: best1 (bestrophin 1 [Source:NCBI gene;Acc:100302038])

HSP 1 Score: 171.014 bits (432), Expect = 3.733e-48
Identity = 108/352 (30.68%), Postives = 182/352 (51.70%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI----TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+     TF +LL RW+ S++KLL+ +FL F   Y  I++ YR  L  + Q+++ FE +  Y +  A L+P+SF+LGF+V+ V+ R+W    S+PW  +   L  + ++G +E + R LR T++RY NL+ +L +  +S  V  RF                P +      + VN+  M            E +  +GL       +F++P +W   L   AR    I+ +     ++  +  ++     L  ++  +VPLVYTQV+ + VY+F ++ +  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSNRVADARLGTFSRLLLRWRGSIYKLLYREFLIFISCYFIISVTYRFLL--NEQQRIYFEKLALYCDNYAELIPVSFVLGFYVTLVVSRWWGQYESVPWPDRLMCLVSSNVHGTDE-RGRMLRRTLMRYANLTGLLILRSVSTAVYMRF----------------PTLQ-----HVVNAGFMTP---------TERSKFDGLVSSHN--KFWVPCVWFANLAVTARSEGRIRDNVVLQLILKELNSLRTQCGRLYGYDWISVPLVYTQVVTVAVYSFFLACLIGRQFLDPQKGYPGHDMDLYVPVFTLLQFFFYAGWLKVAEQLINPFGQDDDDFETNWLIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Xenopus
Match: best1 (bestrophin 1 [Source:NCBI gene;Acc:100302038])

HSP 1 Score: 170.629 bits (431), Expect = 4.338e-48
Identity = 108/352 (30.68%), Postives = 182/352 (51.70%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI----TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+     TF +LL RW+ S++KLL+ +FL F   Y  I++ YR  L  + Q+++ FE +  Y +  A L+P+SF+LGF+V+ V+ R+W    S+PW  +   L  + ++G +E + R LR T++RY NL+ +L +  +S  V  RF                P +      + VN+  M            E +  +GL       +F++P +W   L   AR    I+ +     ++  +  ++     L  ++  +VPLVYTQV+ + VY+F ++ +  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSNRVADARLGTFSRLLLRWRGSIYKLLYREFLIFISCYFIISVTYRFLL--NEQQRIYFEKLALYCDNYAELIPVSFVLGFYVTLVVSRWWGQYESVPWPDRLMCLVSSNVHGTDE-RGRMLRRTLMRYANLTGLLILRSVSTAVYMRF----------------PTLQ-----HVVNAGFMTP---------TERSKFDGLVSSHN--KFWVPCVWFANLAVTARSEGRIRDNVVLQLILKELNSLRTQCGRLYGYDWISVPLVYTQVVTVAVYSFFLACLIGRQFLDPQKGYPGHDMDLYVPVFTLLQFFFYAGWLKVAEQLINPFGQDDDDFETNWLIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Xenopus
Match: best1 (bestrophin 1 [Source:NCBI gene;Acc:100302038])

HSP 1 Score: 170.629 bits (431), Expect = 9.809e-48
Identity = 119/405 (29.38%), Postives = 195/405 (48.15%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI----TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPE-----YLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQ 1247
            +V Y + V+     TF +LL RW+ S++KLL+ +FL F   Y  I++ YR  L  + Q+++ FE +  Y +  A L+P+SF+LGF+V+ V+ R+W    S+PW  +   L  + ++G +E + R LR T++RY NL+ +L +  +S  V  RF                P +      + VN+  M            E +  +GL       +F++P +W   L   AR    I+ +     ++  +  ++     L  ++  +VPLVYTQV+ + VY+F ++ +  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   + ++D NLQ+S +         P      Y N S          V    E  R   Q + RD Q
Sbjct:    2 TVTYSNRVADARLGTFSRLLLRWRGSIYKLLYREFLIFISCYFIISVTYRFLL--NEQQRIYFEKLALYCDNYAELIPVSFVLGFYVTLVVSRWWGQYESVPWPDRLMCLVSSNVHGTDE-RGRMLRRTLMRYANLTGLLILRSVSTAVYMRF----------------PTLQ-----HVVNAGFMTP---------TERSKFDGLVSSHN--KFWVPCVWFANLAVTARSEGRIRDNVVLQLILKELNSLRTQCGRLYGYDWISVPLVYTQVVTVAVYSFFLACLIGRQFLDPQKGYPGHDMDLYVPVFTLLQFFFYAGWLKVAEQLINPFGQDDDDFETNWLIDRNLQVSLLAVDEMHQDVPPLEKDIYWNDSDPQPPYTASTV----ETRRPSYQGSTRDPQ 367
[Close]
BLAST of Bestrophin homolog vs. Ensembl Xenopus
Match: best1 (bestrophin 1 [Source:NCBI gene;Acc:100302038])

HSP 1 Score: 171.4 bits (433), Expect = 1.003e-47
Identity = 108/352 (30.68%), Postives = 182/352 (51.70%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI----TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+     TF +LL RW+ S++KLL+ +FL F   Y  I++ YR  L  + Q+++ FE +  Y +  A L+P+SF+LGF+V+ V+ R+W    S+PW  +   L  + ++G +E + R LR T++RY NL+ +L +  +S  V  RF                P +      + VN+  M            E +  +GL       +F++P +W   L   AR    I+ +     ++  +  ++     L  ++  +VPLVYTQV+ + VY+F ++ +  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSNRVADARLGTFSRLLLRWRGSIYKLLYREFLIFISCYFIISVTYRFLL--NEQQRIYFEKLALYCDNYAELIPVSFVLGFYVTLVVSRWWGQYESVPWPDRLMCLVSSNVHGTDE-RGRMLRRTLMRYANLTGLLILRSVSTAVYMRF----------------PTLQ-----HVVNAGFMTP---------TERSKFDGLVSSHN--KFWVPCVWFANLAVTARSEGRIRDNVVLQLILKELNSLRTQCGRLYGYDWISVPLVYTQVVTVAVYSFFLACLIGRQFLDPQKGYPGHDMDLYVPVFTLLQFFFYAGWLKVAEQLINPFGQDDDDFETNWLIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Xenopus
Match: ifitm3 (interferon induced transmembrane protein 3 [Source:Xenbase;Acc:XB-GENE-5852510])

HSP 1 Score: 171.785 bits (434), Expect = 1.569e-46
Identity = 112/352 (31.82%), Postives = 186/352 (52.84%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI----TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y S V+      F +LL +W+ S++KLL+ +FL F  +YT ++++YR +L  D QK+  FE +  Y +K A  +P++F+LGF+V+ V+ R+W    ++PW  +  FL  + ++G +E   R LR T++RY+NL+ +L    +S  V KRF                P +           D +V+  F   +  +E  + + L  K    ++++P++W   L  KAR    I+       M+N +   ++    L  ++   +PLVYTQV+ + VYTF  + I  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S
Sbjct:    2 TVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFLLFTSLYTTLSVIYRFFLT-DSQKRY-FEKVSMYCDKYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSNVHGRDEY-GRLLRRTLMRYVNLTSLLIFRSVSTAVYKRF----------------PTM-----------DHVVEAGF---MTSDERKLFDSL--KSPHLKYWVPVIWFGNLASKARSEGRIRDSIDLQMMMNEMNKFRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACIIGRQFLDPKQAYPGHDLDLYIPVFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Mouse
Match: Best1 (bestrophin 1 [Source:MGI Symbol;Acc:MGI:1346332])

HSP 1 Score: 162.925 bits (411), Expect = 7.088e-45
Identity = 107/353 (30.31%), Postives = 173/353 (49.01%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESY-----FPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            ++ Y + V+     +F  LL  W+ S++KLL+ +FL F  +Y  I  LYR+ L  D Q  ++FE +  Y +    L+P+SF+LGF+V+ V+ R+W+   ++PW  +      + + G +E + R LR T+IRY  L  VL +  +S  V KRF                P +                      +   E   L+ L     T  F++P +W   L  KA     I+      S++N +  ++     L  ++  ++PLVYTQV+ + VY+F ++ +  +Q + NPN D   +      P+F+I +F  YMGW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TITYTNKVANARLGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQ--LLFEKLALYCDSYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDE-EGRLLRRTLIRYAILGQVLILRSISTSVYKRF----------------PTLH--------------HLVLAGFMTHGEHKQLQKLGLPHNT--FWVPWVWFANLSMKAYLGGRIRDTVLLQSLMNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQFL-NPNKDYPGHEMDLVVPVFTILQFLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Mouse
Match: Best1 (bestrophin 1 [Source:MGI Symbol;Acc:MGI:1346332])

HSP 1 Score: 162.54 bits (410), Expect = 7.153e-44
Identity = 106/353 (30.03%), Postives = 172/353 (48.73%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESY-----FPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            ++ Y + V+     +F  LL  W+ S++KLL+ +FL F  +Y  I  LYR+ L  D Q  ++FE +  Y +    L+P+SF+LGF+V+ V+ R+W+   ++PW  +      + + G +E + R LR T+IRY  L  VL +  +S  V KRF                                +        +   E   L+ L     T  F++P +W   L  KA     I+      S++N +  ++     L  ++  ++PLVYTQV+ + VY+F ++ +  +Q + NPN D   +      P+F+I +F  YMGW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TITYTNKVANARLGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQ--LLFEKLALYCDSYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDE-EGRLLRRTLIRYAILGQVLILRSISTSVYKRF------------------------------PTLHHLVLAGFMTHGEHKQLQKLGLPHNT--FWVPWVWFANLSMKAYLGGRIRDTVLLQSLMNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQFL-NPNKDYPGHEMDLVVPVFTILQFLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Mouse
Match: Best3 (bestrophin 3 [Source:MGI Symbol;Acc:MGI:3580298])

HSP 1 Score: 164.081 bits (414), Expect = 9.805e-44
Identity = 118/399 (29.57%), Postives = 195/399 (48.87%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAV---------------------IAR------ASDSCFPEYLNSSLE 1163
            +V Y S V+      F +LL +W+ S++KLL+ +F+ F ++YT I+L+YRL L     +K  FE +  Y ++ A  +P++F+LGF+V+ V+ R+W    ++PW  +   L  + ++G ++   R LR T++RY+NL+ +L    +S  V KRF                P +           D +V+  F   +  +E  + + L  K    ++++P +W   L  KAR    I+      S++  +   ++    L  ++   +PLVYTQV+ + VYTF    LI   F   T      D++ Y PIF++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S +                      AR      A+D C P +L S+++
Sbjct:    2 TVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTG--AQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMLLISSSVHGSDQ-HGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRF----------------PTM-----------DHVVEAGF---MTADERKLFDHL--KSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYVGHDLDLYVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTIQ 365
[Close]
BLAST of Bestrophin homolog vs. Ensembl Mouse
Match: Best2 (bestrophin 2 [Source:MGI Symbol;Acc:MGI:2387588])

HSP 1 Score: 152.91 bits (385), Expect = 1.599e-40
Identity = 103/353 (29.18%), Postives = 182/353 (51.56%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESY-----FPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F +LL  W+ S++KLLW + L F  +Y  ++  YR +L+ + QK+  FE +V Y ++ A+L+P+SF+LGF+V+ V+ R+W     +P       +    ++G ++ + R  R T++RY  LS VL +  +S  V KRF                P I           D +V+  F   +  EE    E L+   +  ++++P +W + L  +AR+   I+ +S    ++  +   ++    L  ++  ++PLVYTQV+ I VY++ ++ +  +Q + +P    + +      PIF++ +FF Y GW KV    INPFG DD D   + ++D N Q+S
Sbjct:    2 TVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGLYMALSAAYR-FLLAEEQKRY-FEKLVIYCDQYASLIPVSFVLGFYVTLVVHRWWNQYLCMPLPDALMCIVAGTVHGRDD-RGRLYRRTLMRYAGLSAVLILRSVSTAVFKRF----------------PTI-----------DHVVEAGF---MTREERKKFENLNS--SYNKYWVPCVWFSSLAAQARREGRIRDNSALKLLLEELNVFRSKCGMLFHYDWISIPLVYTQVVTIAVYSYFLACLIGRQFL-DPAQGYKDHTLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Mouse
Match: Best2 (bestrophin 2 [Source:MGI Symbol;Acc:MGI:2387588])

HSP 1 Score: 152.91 bits (385), Expect = 1.599e-40
Identity = 103/353 (29.18%), Postives = 182/353 (51.56%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESY-----FPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F +LL  W+ S++KLLW + L F  +Y  ++  YR +L+ + QK+  FE +V Y ++ A+L+P+SF+LGF+V+ V+ R+W     +P       +    ++G ++ + R  R T++RY  LS VL +  +S  V KRF                P I           D +V+  F   +  EE    E L+   +  ++++P +W + L  +AR+   I+ +S    ++  +   ++    L  ++  ++PLVYTQV+ I VY++ ++ +  +Q + +P    + +      PIF++ +FF Y GW KV    INPFG DD D   + ++D N Q+S
Sbjct:    2 TVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGLYMALSAAYR-FLLAEEQKRY-FEKLVIYCDQYASLIPVSFVLGFYVTLVVHRWWNQYLCMPLPDALMCIVAGTVHGRDD-RGRLYRRTLMRYAGLSAVLILRSVSTAVFKRF----------------PTI-----------DHVVEAGF---MTREERKKFENLNS--SYNKYWVPCVWFSSLAAQARREGRIRDNSALKLLLEELNVFRSKCGMLFHYDWISIPLVYTQVVTIAVYSYFLACLIGRQFL-DPAQGYKDHTLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVS 318
[Close]
BLAST of Bestrophin homolog vs. UniProt/SwissProt
Match: sp|Q8N1M1|BEST3_HUMAN (Bestrophin-3 OS=Homo sapiens OX=9606 GN=BEST3 PE=1 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 3.845e-44
Identity = 119/399 (29.82%), Postives = 193/399 (48.37%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAV---------------------IAR------ASDSCFPEYLNSSLE 1163
            +V Y S V+      F +LL +W+ S++KLL+ +F+ F ++YT I+L+YRL L     +K  FE +  Y ++ A  +P++F+LGF+V+ V+ R+W    ++PW  +  FL  + ++G +E   R LR T++RY+NL+ +L    +S  V KRF                P +        + +DE  ++ F  L              K    ++++P +W   L  KAR    I+      S++  +   ++    L  ++   +PLVYTQV+ + VYTF    LI   F   T      D++ Y PIF++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S +                      AR      A+D C P +L S+++
Sbjct:    2 TVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTG--VQKRYFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMFLISSSVHGSDE-HGRLLRRTLMRYVNLTSLLIFRSVSTAVYKRF----------------PTMDHVVEAGFMTTDE--RKLFNHL--------------KSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYAGHDLDLYIPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFLGSTVQ 365
[Close]
BLAST of Bestrophin homolog vs. UniProt/SwissProt
Match: sp|O76090|BEST1_HUMAN (Bestrophin-1 OS=Homo sapiens OX=9606 GN=BEST1 PE=1 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.766e-43
Identity = 108/354 (30.51%), Postives = 180/354 (50.85%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQ--FFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            ++ Y S V+     +F +LL  W+ S++KLL+ +FL F L Y  I  +YRL L ++ Q  ++FE +  Y +    L+P+SF+LGF+V+ V+ R+W    ++PW  +   L    + G +E + R LR T+IRY NL  VL +  +S  V KRF                            ++  +V+  F   +   E   LE L    + P   F++P +W   L  KA     I+      S++N +  ++    +L  ++  ++PLVYTQV+ + VY+F ++ +  +Q +      P  +++   P+F+  +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQ--LMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDE-QGRLLRRTLIRYANLGNVLILRSVSTAVYKRF---------------------------PSAQHLVQAGF---MTPAEHKQLEKL----SLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. UniProt/SwissProt
Match: sp|Q8NFU0|BEST4_HUMAN (Bestrophin-4 OS=Homo sapiens OX=9606 GN=BEST4 PE=2 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.247e-43
Identity = 109/367 (29.70%), Postives = 184/367 (50.14%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQ--------QTITNPN-------IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+      F  LL RW+ S++KLL+ +FL F  +Y  +++ YRL L +  +++ V+  +  Y N++A L+PLSF+LGF+V+ V+ R+W+   SIP   +   +  A ++G ++ + R LR T+IRY NL+ VL +  +S +V KRF   +      +++        R++   + SD      F K                     +++P +W T L  +AR+   I+ D     ++  +   +     L  ++  ++PLVYTQV+ I VY+F    L+   F +        Q +  P         D + Y P+ ++ +FF Y GW KV    INPFG DD D   ++++D NLQ+S
Sbjct:    2 TVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQ--EQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQ-RGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTME-----HVVDAGFMSQEERKKFESLKSD------FNK---------------------YWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVS 333
[Close]
BLAST of Bestrophin homolog vs. UniProt/SwissProt
Match: sp|O88870|BEST1_MOUSE (Bestrophin-1 OS=Mus musculus OX=10090 GN=Best1 PE=2 SV=3)

HSP 1 Score: 162.54 bits (410), Expect = 5.003e-43
Identity = 106/353 (30.03%), Postives = 172/353 (48.73%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESY-----FPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            ++ Y + V+     +F  LL  W+ S++KLL+ +FL F  +Y  I  LYR+ L  D Q  ++FE +  Y +    L+P+SF+LGF+V+ V+ R+W+   ++PW  +      + + G +E + R LR T+IRY  L  VL +  +S  V KRF                                +        +   E   L+ L     T  F++P +W   L  KA     I+      S++N +  ++     L  ++  ++PLVYTQV+ + VY+F ++ +  +Q + NPN D   +      P+F+I +F  YMGW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TITYTNKVANARLGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQ--LLFEKLALYCDSYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDE-EGRLLRRTLIRYAILGQVLILRSISTSVYKRF------------------------------PTLHHLVLAGFMTHGEHKQLQKLGLPHNT--FWVPWVWFANLSMKAYLGGRIRDTVLLQSLMNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQFL-NPNKDYPGHEMDLVVPVFTILQFLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. UniProt/SwissProt
Match: sp|Q6UY87|BEST1_MACFA (Bestrophin-1 OS=Macaca fascicularis OX=9541 GN=BEST1 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 5.871e-43
Identity = 107/354 (30.23%), Postives = 179/354 (50.56%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQ--FFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            ++ Y S V+     +F +LL  W+ S++KLL+ +F  F L Y  I  +YRL L ++ Q  ++FE +  Y +    L+P+SF+LGF+V+ V+ R+W    ++PW  +   L    + G +E + R LR T+IRY NL  VL +  +S  V KRF                            ++  +V+  F   +   E   LE L    + P   F++P +W   L  KA     I+      S++N +  ++    +L  ++  ++PLVYTQV+ + VY+F ++ +  +Q +      P  +++   P+F+  +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFFIFLLCYYIIRFIYRLALTEEQQ--LMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDE-QGRLLRRTLIRYANLGNVLILRSVSTAVYKRF---------------------------PSAQHLVQAGF---MTPAEHKQLEKL----SLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. TrEMBL
Match: A0A267FRV1 (Bestrophin homolog OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021055g1 PE=3 SV=1)

HSP 1 Score: 294.664 bits (753), Expect = 2.218e-90
Identity = 160/434 (36.87%), Postives = 244/434 (56.22%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKS---RQLRLTIIRYLNLSWVLTMMKLSDKVRKRF--WIKK-ENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLE-----GLDDKPT-TPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI-------------------TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFE------------AERSEIQTN 1232
             V Y S +   +R+ F+KLLFRW  SVW+LLW DFL F LIY  +  LY L L  DP+ K  FE  V+++   +  VPLSF+LGFFVS+V+ R+W++  SIPW+  P+F CQA+L+     +S   R+LR+T++RYLNL+W+L M  +S  +R+RF  W  K + K +        K++ R RL  VNSDE V++YFG+L+ + E+           D+K T  P++++PL+WAT+L+ +AR  D I  +  C  +++ +Q  + +L  + +F  FN+PLVY QV +  VY +L+S I S Q +                    N    +  + P+    +F  Y+GWFK+G+  +NP G D+ D+P+ ++L+ NL+ +  +  A DS FP  L  S E       E L  F+            AE++E Q N
Sbjct:    2 GVSYASKIYTKDRLFFIKLLFRWSGSVWQLLWLDFLVFILIYVSLAALYHLVLYNDPKMKATFEITVSWLKTVSTAVPLSFMLGFFVSSVVERWWSVCMSIPWLQTPSFYCQALLDSPKTYRSDLARKLRITVLRYLNLAWILMMAGVSLHIRERFGYWQPKWKTKKY--------KLTWRRRLKMVNSDEKVQKYFGQLVTDNEIAAFGRSAELSNDNKHTYDPEYWLPLVWATRLIRRARNLDCIADERHCLWLIDTVQKFRYDLGTIWMFTEFNIPLVYVQVAVTAVYLYLVSTIISTQLMDSDIVQSSTAILNVNGTYQRNTGQQVLGFIPVLGGLEFIFYLGWFKIGMCLLNPMGKDEADIPMSDILNSNLKAAIKMGGAEDSIFPRGLTDSGEAEEAQVAEFLCCFDFAGVHAHDKFGSAEQTERQLN 427
[Close]
BLAST of Bestrophin homolog vs. TrEMBL
Match: A0A267GVJ0 (Bestrophin homolog OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003074g1 PE=3 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 4.641e-90
Identity = 160/434 (36.87%), Postives = 244/434 (56.22%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKS---RQLRLTIIRYLNLSWVLTMMKLSDKVRKRF--WIKK-ENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLE-----GLDDKPT-TPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI-------------------TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFE------------AERSEIQTN 1232
             V Y S +   +R+ F+KLLFRW  SVW+LLW DFL F LIY  +  LY L L  DP+ K  FE  V+++   +  VPLSF+LGFFVS+V+ R+W++  SIPW+  P+F CQA+L+     +S   R+LR+T++RYLNL+W+L M  +S  +R+RF  W  K + K +        K++ R RL  VNSDE V++YFG+L+ + E+           D+K T  P++++PL+WAT+L+ +AR  D I  +  C  +++ +Q  + +L  + +F  FN+PLVY QV +  VY +L+S I S Q +                    N    +  + P+    +F  Y+GWFK+G+  +NP G D+ D+P+ ++L+ NL+ +  +  A DS FP  L  S E       E L  F+            AE++E Q N
Sbjct:    2 GVSYASKIYTKDRLFFIKLLFRWSGSVWQLLWLDFLVFILIYVSLAALYHLVLYNDPKMKATFEITVSWLKTVSTAVPLSFMLGFFVSSVVERWWSVCMSIPWLQTPSFYCQALLDSPKTYRSDLARKLRITVLRYLNLAWILMMAGVSLHIRERFGYWQPKWKTKKY--------KLTWRRRLKMVNSDEKVQKYFGQLVTDNEIAAFGRSAELSNDNKHTYDPEYWLPLVWATRLIRRARNLDCIADERHCLWLIDTVQKFRYDLGTIWMFTEFNIPLVYVQVAVTAVYLYLVSTIISTQLMDSDIVQSSTAILNVNGTYQRNTGQQVLGFIPVLGGLEFIFYLGWFKIGMCLLNPMGKDEADIPMSDILNSNLKAAIKMGGAEDSIFPRGLMDSGEAEEAQVAEFLCCFDFAGVHAHDKFGSAEQTERQLN 427
[Close]
BLAST of Bestrophin homolog vs. TrEMBL
Match: A0A267FSL4 (Bestrophin homolog OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030079g1 PE=3 SV=1)

HSP 1 Score: 285.419 bits (729), Expect = 1.068e-86
Identity = 153/411 (37.23%), Postives = 235/411 (57.18%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKS---RQLRLTIIRYLNLSWVLTMMKLSDKVRKRF--WIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTV------LEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ-----------------TITNP-NIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFE 1205
            +V Y S +  ++R+ F++LLFRW  SVW+LLW D+L F L+Y G+ +LY   L    + K  FE  VA++      VPLSF+LGFFVS V GR+W++  SIPW+   +F CQA+++     +S   R+LR+T++RYLNL+W+L M  +S  +++RF  W  K  K F+L        + + RL  +NSD  V++YFG+L+ ++EM        L   +D    P++++PLMWAT+L+ +A+    I  +     +VN+I + + NL  + ++  FN+PLVYTQV +  VY FL S + S Q                 T+T    +++ SY P+    +F  Y+GWFK+G+  +NP G D  D+P+ ++L+ NL+ S  I  A DS FP  L  S E       E L  F+
Sbjct:    2 AVSYASQIYTDDRLFFVRLLFRWAGSVWQLLWIDYLIFTLMYVGLAVLYFNLLYTHAELKATFEVTVAWLKAIRTSVPLSFMLGFFVSAVFGRWWSVCTSIPWLHSSSFYCQALVDSPKTSRSDLARKLRITVLRYLNLAWILMMAGVSLHIKERFGYWQPKWRK-FQL--------TWQRRLQLINSDSKVQKYFGQLVTDKEMQAFAKSAELSNDNDHSYDPEYWLPLMWATRLIRRAKNLGCIADERQYLFLVNIINNFRGNLGTVWMYTEFNIPLVYTQVAVTAVYLFLASTLISTQLVDSDILLLENFPSRNGTVTRTLGLNLLSYLPVIGSLEFIFYLGWFKIGMCLLNPMGKDKADIPMSDILNSNLKASVKIGGAEDSIFPGGLTDSGEAEEAQVAEFLRSFD 403
[Close]
BLAST of Bestrophin homolog vs. TrEMBL
Match: A0A267GVI2 (Bestrophin homolog OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001426g2 PE=3 SV=1)

HSP 1 Score: 284.648 bits (727), Expect = 1.985e-86
Identity = 153/411 (37.23%), Postives = 235/411 (57.18%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKS---RQLRLTIIRYLNLSWVLTMMKLSDKVRKRF--WIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTV------LEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ-----------------TITNP-NIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFE 1205
            +V Y S +  ++R+ F++LLFRW  SVW+LLW D+L F L+Y G+ +LY   L    + K  FE  VA++      VPLSF+LGFFVS V GR+W++  SIPW+   +F CQA+++     +S   R+LR+T++RYLNL+W+L M  +S  +++RF  W  K  K F+L        + + RL  +NSD  V++YFG+L+ E+EM        L   +D    P++++PLMWAT+L+ +A+    I  +     +VN+I + + +L  + ++  FN+PLVYTQV +  VY FL S + S Q                 T+T    +++ SY P+    +F  Y+GWFK+G+  +NP G D  D+P+ ++L+ NL+ S  I  A DS FP  L  S E       E L  F+
Sbjct:    2 AVSYASQIYTDDRLFFVRLLFRWAGSVWQLLWIDYLIFTLMYVGLAVLYFNLLYTHAELKATFEVTVAWLKAIRTSVPLSFMLGFFVSAVFGRWWSVCTSIPWLHSSSFYCQALVDSPKTSRSDLARKLRITVLRYLNLAWILMMAGVSLHIKERFGYWQPKWRK-FQL--------TWQRRLQLINSDSKVQKYFGQLVTEKEMQAFAKSAELSNDNDHSYDPEYWLPLMWATRLIRRAKNLGCIADERQYLFLVNIINNFRGDLGTVWMYTEFNIPLVYTQVAVTAVYLFLASTLISTQLVDSDILLLENFPSRNGTVTRTLGLNLLSYLPVIGSLEFIFYLGWFKIGMCLLNPMGKDKADIPMSDILNSNLKASVKIGGAEDSIFPGGLTDSGEAEEAQVAEFLRSFD 403
[Close]
BLAST of Bestrophin homolog vs. TrEMBL
Match: A0A267FBA9 (Bestrophin homolog OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028808g1 PE=3 SV=1)

HSP 1 Score: 284.263 bits (726), Expect = 3.202e-86
Identity = 152/421 (36.10%), Postives = 238/421 (56.53%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKS---RQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTV------LEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI---------------------TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTN 1232
            +V Y S +   E++ FLKLLFRW  SVW+LLW DF +F L Y G+ ++Y   L   P +++ FE+++         VPLSF+LGFFVS+VL R+W++  +IPWM  PAF  Q ++   ++ KS   R++R+T++RY NL+W+L M  +S  ++ RF   +   + E       K++ R+RL  +NSD  V++YFGKLI EEEM V      L    D   TP++++PL WA +++ +A+    I  +     MV + Q+ +N+L  +  ++ FN+PLVYTQV +  VY FL S + S Q I                     +   +++ S+ PI    +F  Y+GWFK+G+  +NP G D  DLPL E+L+ NL++S  +  A DS FP  L  + E      +++   F+   +E+ +N
Sbjct:    2 AVSYASNIYTKEKLFFLKLLFRWSGSVWQLLWVDFFFFALAYVGLAVMYHQLL--SPTRRITFEAVIRSTGSIRTSVPLSFMLGFFVSSVLSRWWSMCMAIPWMHAPAFYAQGLIESCHKSKSDIARKVRITVLRYFNLAWILMMATISLHIKHRFGFMRPKNTREF------KLTWRQRLELINSDAKVQKYFGKLITEEEMQVFARSAELSKDTDWTYTPEYWLPLGWANRIMRRAKNIGCIADERQFLWMVTIAQNFRNDLGIVWTYSEFNIPLVYTQVAVTAVYIFLFSTLLSTQLIESEVGLDRHSLVNGSRVDEPGSTTGVEVLSHIPILGSLEFIFYLGWFKIGMCLMNPLGKDKADLPLSEILNSNLKVSVKLGGAEDSLFPPGLTDNGEPEEQQLVDLFRTFDF--NEVHSN 412
[Close]
BLAST of Bestrophin homolog vs. Ensembl Cavefish
Match: best1 (bestrophin-2 [Source:NCBI gene;Acc:103045999])

HSP 1 Score: 185.267 bits (469), Expect = 2.820e-51
Identity = 117/353 (33.14%), Postives = 192/353 (54.39%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTP-QFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQ----TITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+     TF  LL RW+ S++KLL+ + L F ++Y  I++LYR  L  D +++ +FE +  Y ++ A L+P+SF+LGF+V+ V+ R+W    S+PW  + A L  A + G +E  +R +R +++RY NLS VL    +S  V KRF                P I           + +V+   G + +EE       LDD P+   +F++P MW   L  +AR    I +D   S+++N +  +++    L  ++  ++PLVYTQV+ + VY+F ++ +  +Q    T      D++ Y P+F++ +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSRRVADARLGTFSHLLLRWKGSIYKLLYRELLIFTVLYCTISVLYRFLL--DNEQRRLFEKLAIYCDQYAQLIPVSFVLGFYVTLVVSRWWGQFESVPWPDRLAALVGAYVRGADE-GARLIRRSLVRYANLSGVLIYRSISTAVYKRF----------------PTI-----------EHLVQA--GLMTVEERRQ----LDDLPSPHNKFWVPCMWFVNLALRARTEGRINNDVALSAILNELNTLRSQCMKLYSYDWISLPLVYTQVVTVAVYSFFLACLIGRQFLDPTQGYSGHDLDFYLPVFTLLQFFFYVGWLKVAEQLINPFGEDDDDFETNWLVDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Cavefish
Match: best2 (bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127])

HSP 1 Score: 176.407 bits (446), Expect = 1.864e-48
Identity = 116/352 (32.95%), Postives = 182/352 (51.70%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F KLL  W+ S++K+L+ + L FF++YT I++ YR +L+ D QK+  FE +  Y N  A+L+P+SF+LGF+V+ ++ R+W+   SIP       +    L G +E + R LR T++RY +L+ +L +  +S  V KRF                P +           D +V+  F   +  EE    E L       +++IP +W T L   AR    IK D+T   ++  +   +     L  ++  +VPLVYTQV+ + VY+F    LI   F   +   P  D++ Y PIF++ +FF Y GW KV    INPFG DD D   + ++D N Q+S
Sbjct:    2 TVTYTARVANARFCGFSKLLLAWRGSIYKVLYKEVLAFFILYTAISITYRFFLL-DEQKRY-FEKLSIYCNHYASLIPMSFVLGFYVTLIVNRWWSQYTSIPLPDSIMCVLSGGLQGGDE-RGRLLRRTLMRYASLASLLILRSVSTAVFKRF----------------PTM-----------DHVVEAGF---MTREERKKFESLHSPYN--KYWIPCVWFTNLAAVARSEGRIKDDNTYKLLLEELNKFRGKCSLLFHYDMISVPLVYTQVVTLAVYSFFLVCLIGRQFLDPSQAYPGHDLDLYVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNFQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Cavefish
Match: best4 (bestrophin 4 [Source:ZFIN;Acc:ZDB-GENE-070912-6])

HSP 1 Score: 163.31 bits (412), Expect = 1.047e-45
Identity = 107/354 (30.23%), Postives = 175/354 (49.44%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXX-XXYTFLISEIFSQQTITNPNID-----IESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+      F KLLFRW+ S++KLL+ +FL F  +Y+  +L+YR  L    Q+  +FE    + NK A L+P+SF+L F+V+    R+W    S P       +    ++G +E + R LR T++RY NLS VL +  +S +V KRF                                 ++      + EEEM  L+ L       ++++PL W   L  KAR+   +K D     +++ + + +     L  ++  ++PLVYTQ ++   VY+F    +  +Q +     D     ++ Y P+F++ +FF Y GW KVG   INPFG DD D   ++++D N+Q+S
Sbjct:    2 TVSYTLEVANARFAGFTKLLFRWKGSIYKLLYKEFLLFCFLYSLFSLIYRCVLTDGQQE--LFERAARHCNKFAKLIPMSFVL-FYVTQAFQRWWGQYTSFPLPDNLMMVVSGNVHGSDE-RGRLLRRTLMRYANLSSVLILRSISTRVHKRF-------------------------------PTLQHIVDAFMTEEEMRTLDHL--YSDFNKYWMPLTWFANLASKAREEGRVKDDIALRLLMDELNNYRGKCSMLFHYDWISIPLVYTQQVVTLAVYSFFAFCLIGRQFVKPERADELKVHVDLYVPVFTLLEFFFYAGWLKVGELIINPFGEDDDDFETNQLIDRNIQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Cavefish
Match: best1 (bestrophin-2 [Source:NCBI gene;Acc:103045999])

HSP 1 Score: 164.466 bits (415), Expect = 6.888e-44
Identity = 111/349 (31.81%), Postives = 176/349 (50.43%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTP-QFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+     TF  LL RW+ S++KLL+ + L F ++Y  I++LYR  L  D +++ +FE +  Y ++ A L+P+SF+LGF+V+ V+ R+W    S+PW  + A L  A + G +E  +R +R +++RY NLS VL    +S  V KRF                P I           + +V+   G + +EE       LDD P+   +F++P MW   L  +AR    I +D   S+++N++         +AV++ F                 LI   F   T      D++ Y P+F++ +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSRRVADARLGTFSHLLLRWKGSIYKLLYRELLIFTVLYCTISVLYRFLL--DNEQRRLFEKLAIYCDQYAQLIPVSFVLGFYVTLVVSRWWGQFESVPWPDRLAALVGAYVRGADE-GARLIRRSLVRYANLSGVLIYRSISTAVYKRF----------------PTI-----------EHLVQA--GLMTVEERRQ----LDDLPSPHNKFWVPCMWFVNLALRARTEGRINNDVALSAILNVV--------TVAVYSFF--------------LACLIGRQFLDPTQGYSGHDLDFYLPVFTLLQFFFYVGWLKVAEQLINPFGEDDDDFETNWLVDRNLQVS 292
[Close]
BLAST of Bestrophin homolog vs. Ensembl Cavefish
Match: BEST3 (bestrophin 3 [Source:HGNC Symbol;Acc:HGNC:17105])

HSP 1 Score: 95.5153 bits (236), Expect = 7.514e-21
Identity = 68/236 (28.81%), Postives = 107/236 (45.34%), Query Frame = 3 
Query:  642 IMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAV--------IAR-------------------ASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFG 1256
            +  EE  + E L  K    +++IP +W T L  KARK   I+      +++  +   +     L  ++   +PLVYTQV+ + VYTF  + I  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S +        I R                   A+D C P +L S+ +      + + E  + E ++IQ   R     G
Sbjct:   85 MTPEEKKIFENL--KSPHLKYWIPTVWFTNLASKARKDGRIQDSVDLQTILTEMNRFRTWCSTLFGYDWVGIPLVYTQVVTLAVYTFFSASIIGRQFLDPAQGYPGHDLDLYVPVFTLLQFFFYSGWLKVAEQLINPFGEDDDDFEANWCIDRNLQVSLMAVDEMHMNIPRMTKDVYWNDPDVRPPYTRAAADYCIPSFLGSTTD------MGLSEILQNEDTDIQNARRQGSVLG 312

HSP 2 Score: 66.2402 bits (160), Expect = 2.090e-11
Identity = 33/85 (38.82%), Postives = 60/85 (70.59%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGF 314
            +V Y S V+      F +LL RW+ S++KLL+ +FL+F L+Y+ ++L+YR +++ DPQ++ +FE +  Y ++ A  +P++F+LGF
Sbjct:    2 TVTYSSKVANATFFGFHRLLLRWRGSIYKLLYREFLFFALLYSILSLVYR-WVLTDPQRR-LFEKLSIYCDRYAEQIPVTFVLGF 84
[Close]
BLAST of Bestrophin homolog vs. Ensembl Sea Lamprey
Match: best1 (bestrophin 1 [Source:ZFIN;Acc:ZDB-GENE-110411-214])

HSP 1 Score: 172.17 bits (435), Expect = 9.887e-50
Identity = 108/351 (30.77%), Postives = 189/351 (53.85%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN---PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F +LL RW+ S++KLL+ +F  F +++  ++ +YR Y++++ QK+  FE +  Y NK A L+P+SF+LGF+V+ V+ R+W+   S+PW  +   L    ++G +E + R LR T++RY NLS +L +  +S  V KRF                P +           D +V+  F   +   E    +GL  +    +F++P +W   L  +A+    I  + +   ++N + + ++    L  ++  ++PLVYTQV+ + VY+F ++ +  +Q +     P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   +  +D NLQ+S
Sbjct:    2 TVTYSNKVANARLGGFSQLLLRWKGSIYKLLYKEFCIFLVLFFTLSSVYR-YMLQENQKRY-FEKLAMYCNKYAELIPVSFVLGFYVTLVVSRWWSQYESLPWPDRLMHLVACNVHGADE-RGRLLRRTLMRYANLSALLILRSVSTAVYKRF----------------PTM-----------DHVVEAGF---MTPAERRKFDGL--RSPHNKFWVPCVWFANLAAQAKAEGRILDNVSLQLILNDLNNFRSCCSMLYSYDWVSIPLVYTQVVTVAVYSFFLACLIGRQFLGPQGYPGHDMDLYIPVFTLLQFFFYAGWLKVAEQLINPFGEDDDDFEANWCVDRNLQVS 317
[Close]
BLAST of Bestrophin homolog vs. Ensembl Sea Lamprey
Match: best2 (bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127])

HSP 1 Score: 166.777 bits (421), Expect = 2.390e-46
Identity = 105/352 (29.83%), Postives = 183/352 (51.99%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F KLL +W+ S++KLL+ +F  +F++Y  ++L  R  L +D  +K +FE +  Y N  A L+P+    GF+V+ V+ R+W+   SIP   +        ++G +E + R LR T++RY NLS +L +  +S  V KRF                P +           D +V+  F   +  +E    +GL  + +  ++++P +W + L  +AR    ++ D     +++ +   + N   L  ++  +VPLVYTQV+ + VY+F ++ +  +Q +      P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYTNRVANARLCGFSKLLLQWRGSIYKLLYREFSIYFMMYAALSLTSRFALSED--QKRLFEKLSLYCNNYANLIPVRSCSGFYVALVVSRWWSQYTSIPTPDRLMCAVAGTVHGVDE-RGRLLRRTLMRYANLSALLILRSVSTAVYKRF----------------PTM-----------DHVVEAGF---MTRDERKRFDGL--RCSYNKYWVPCVWLSSLAAQARAEGRVRDDVALKLLLDEMNVYRGNCGMLFHYDWISVPLVYTQVVTVAVYSFFLACLIGRQFLDPAQGYPGHDMDLYIPVFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNLQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Medaka
Match: BEST3 (bestrophin 3 [Source:NCBI gene;Acc:101172121])

HSP 1 Score: 182.57 bits (462), Expect = 1.212e-50
Identity = 124/407 (30.47%), Postives = 209/407 (51.35%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTI----TNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVI---------------------AR------ASDSCFPEYLNSSLENSVVNKLE 1187
            +V Y S V+    ++F +LL RW+ S++KLL+ +FL F L+YT ++L+YRL L +  Q+K +FE +  Y ++ A  +P++F+LGF+V+ V+ R+W    ++PW  +  F   + + G +E   R LR T++RY+NL+ +L    +S  V KRF                P I           D +V+  F   +  EE  V E +  +    +++IP++W + L  +AR+   I+      +++N +   +     L  ++   +PLVYTQV+ + VYTF  + I  +Q +     +P  D++ Y P+F++ +FF Y GW KV    INPFG DD D   + ++D NLQ+S +                      AR      A+D C P +L S+ +  + + LE
Sbjct:    2 TVTYSSKVANATFLSFHRLLLRWRGSIYKLLYREFLLFALLYTILSLIYRLALSE--QQKRLFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFLNLPWPDRLMFHISSCVQGKDEY-GRLLRRTLVRYINLTSLLIFRSVSTAVCKRF----------------PTI-----------DHVVEAGF---MTPEERKVFENI--RSPHLKYWIPVVWFSNLASRARQEGRIQDAIDLQNILNEMNTFRTWCATLFGYDWVGIPLVYTQVVTLAVYTFFFACIIGRQFLDPAQGHPGHDLDLYVPVFTLLQFFFYSGWLKVAEQLINPFGEDDDDFEANWIIDRNLQVSLLAVDEMHMNLPHMTKDIYWNDSDARPPYTLAAADYCIPSFLGSTTDMGLSDILE 373
[Close]
BLAST of Bestrophin homolog vs. Ensembl Medaka
Match: best2 (bestrophin 2 [Source:NCBI gene;Acc:110015962])

HSP 1 Score: 175.637 bits (444), Expect = 2.481e-48
Identity = 113/352 (32.10%), Postives = 180/352 (51.14%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTF----LISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y + V+      F KLL  W+ S++K+L+ +FL FF++Y  I++ YR +L +D  +K  FE +  Y N  A+L+P+SF+LGF+V+ ++ R+W    SIP   +   +    L G++E + R LR +++RY +LS +L +  +S  V KRF                P +           D +V+  F   +  EE    EGL       +++IP +W T L   AR    I+ D     ++  +   +     L  ++  +VPLVYTQV+ + VY+F    LI   F   +      D++ Y PIF++ +FF Y GW KV    INPFG DD D   + ++D N Q+S
Sbjct:    2 TVTYTARVANARFCGFSKLLLAWKGSIYKVLYKEFLAFFVLYAAISITYRFFLYED--QKRYFEKLAIYCNHYASLIPMSFVLGFYVTLIVNRWWNQYTSIPLPDRIMCVLSGGLQGNDE-RGRLLRRSMMRYASLSALLILRSVSTAVFKRF----------------PTM-----------DHVVEAGF---MTREERKKFEGLHSPYN--KYWIPCVWFTNLAAVARCEGRIRDDHALKLLLEELNAFRGKCSLLFHYDMVSVPLVYTQVVTLAVYSFFLVCLIGRQFLDPSQGYQGHDLDLYVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNFQVS 318
[Close]
BLAST of Bestrophin homolog vs. Ensembl Medaka
Match: best1 (bestrophin-2 [Source:NCBI gene;Acc:101167866])

HSP 1 Score: 165.236 bits (417), Expect = 4.035e-44
Identity = 108/352 (30.68%), Postives = 184/352 (52.27%), Query Frame = 3 
Query:   60 SVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITN----PNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQIS 1103
            +V Y   V+     TF  LL RW+ S++KLL+ + + F L+Y  ++L+Y  Y++ + Q+++ FE +  Y ++ A L+P+SF+L F+V+ V+ R+W    +IPW  + A L    + G +E  SR  R  ++RY+NLS VL    +S  V KRF                             ++ +V+     L+  EE+   E L       +F++P MW   L  +AR    I +D   ++++  +  ++     L  ++  ++PLVYTQV  + VY+F ++ +  +Q +      P  D++ Y P+F++ +FF Y+GW KV    INPFG DD D   + ++D NLQ+S
Sbjct:    2 TVTYSRRVADAGLGTFFHLLLRWKGSIYKLLYRELIIFTLLYYFLSLVY--YMLDNDQRRL-FERLSIYCDRYAELIPVSFVL-FYVTLVVSRWWGQFENIPWPDRLAALVGGHVRGTDEA-SRLTRRALMRYVNLSGVLIYRSVSTAVYKRF---------------------------PTTEHLVQ----ALMTSEELRHFEELPSPHN--KFWVPCMWFVNLALRARTEGRINNDVALTAILTELNSLRAKCMKLYGYDWISLPLVYTQVATVAVYSFFLACLIGRQFLDPAQGYPGHDVDFYLPVFTLLQFFFYVGWLKVAEQLINPFGEDDDDFETNWVVDRNLQVS 315
[Close]
BLAST of Bestrophin homolog vs. Planmine SMEST
Match: SMESG000016291.1 (SMESG000016291.1)

HSP 1 Score: 823.543 bits (2126), Expect = 0.000e+0
Identity = 413/414 (99.76%), Postives = 413/414 (99.76%), Query Frame = 3 
Query:   48 MQLKSVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDDMFIKS 1289
            MQLKSVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKD QKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQVIIIVVYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDDMFIKS
Sbjct:    1 MQLKSVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDIQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQVIIIVVYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDDMFIKS 414
[Close]
BLAST of Bestrophin homolog vs. Planmine SMEST
Match: SMESG000016290.1 (SMESG000016290.1)

HSP 1 Score: 469.159 bits (1206), Expect = 5.968e-164
Identity = 239/409 (58.44%), Postives = 305/409 (74.57%), Query Frame = 3 
Query:   69 YLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKE-NKSFELL--NKNLPKIS----IRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDD 1274
            YLSA+SLNER+ FLKLL RWQ S+WKLLW DF+WF + Y+ IT+LYR+YLI DP KK  FESIV Y+NK A +VPLSFLLGFFVSTVLGR+W IVNSIPWM+KPAF CQA++  D E   + +RLTI+RY+NL+W+L++M LS KV++RF I    NK    L  + NL  IS    +R+++ K+NSDE+VK++FGKLIME+E+ V E ++     P F  PLMW T+L+EKA +  +IKS   C ++ NM+QD K+NL NLAV N FNVPLVYTQVIIIVVYTFLISE+ S+Q I+N NI I SYFPIF+IFKF IYMGWFK+GL+FINPFGSD +DLPLDE+LDYNL+IS+ IA   D  +PEYL  + ++   +  + LEKF     E   N + +++  +K Y D
Sbjct:    2 YLSAISLNERIKFLKLLLRWQGSLWKLLWVDFIWFSVFYSAITMLYRMYLIHDPNKKEAFESIVIYLNKVAGIVPLSFLLGFFVSTVLGRWWKIVNSIPWMTKPAFTCQALITEDTEPLCKDIRLTIVRYMNLAWILSVMYLSTKVKQRFIITSTTNKIISCLKDSPNLNTISKGKNVRKKMEKINSDEIVKKHFGKLIMEDEIRVFENVEHMSNKPSFITPLMWTTRLIEKAHRDGFIKSQGVCFNLFNMVQDFKSNLSNLAVHNEFNVPLVYTQVIIIVVYTFLISELLSEQIISNENILIGSYFPIFAIFKFIIYMGWFKMGLSFINPFGSDPSDLPLDEILDYNLEISSTIASGEDFYYPEYLTKTFKDPEDSPWKQLEKFRLLVPE-SFNVKKEEKCCSKTYVD 409
[Close]
BLAST of Bestrophin homolog vs. Planmine SMEST
Match: SMESG000016298.1 (SMESG000016298.1)

HSP 1 Score: 459.914 bits (1182), Expect = 7.998e-160
Identity = 235/433 (54.27%), Postives = 305/433 (70.44%), Query Frame = 3 
Query:   48 MQLKSVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGITLLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWI----KKENKSFELLNKNLPK-ISIRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNID----------------IESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDDMFI 1283
            M  K+VKYLSA+SLNER+ FLKLL RWQ S+WKLLWADFLWF   Y GITLLYRLYLI DP  K  FESIV YMNK AALVPLSFLLGFFVSTVLGR+W IV SIPWM+KPAF CQA++ G +    + +RLTI+RY+NL+W+L++M LS KV+ RF I     K+    ++L    PK I+IR+++ K+NSD +VK++FG LIMEEEM V E  + +   P + +PLMWATKL+EK+ KA YI+S+     + N++Q+ + +L  L+V+N FNVPLVYTQVIIIVVYTFLISEIF+QQ I +  I                 +++Y P+F IFKF IYMGWFKVGL+FINPFG + +DLPLDE+LDYNL++SA +A   D  FPEYL+ + E+   ++ ++ E F +    +        + GN+   +M I
Sbjct:    1 MYTKAVKYLSAISLNERIKFLKLLLRWQGSLWKLLWADFLWFICSYIGITLLYRLYLIHDPVNKGRFESIVTYMNKVAALVPLSFLLGFFVSTVLGRWWEIVMSIPWMTKPAFTCQALICGGDRNTCKNIRLTIVRYMNLAWILSLMNLSQKVKLRFLIPITEGKKTIKMKMLKHEKPKPITIRQKMMKINSDVLVKKFFGTLIMEEEMKVFENFEKQSKKPTYIVPLMWATKLIEKSYKAGYIESERVFLHIFNLVQEFRGSLGTLSVYNEFNVPLVYTQVIIIVVYTFLISEIFTQQLIDHNLIHKKTLYQNSTFISITAALDNYLPVFGIFKFLIYMGWFKVGLSFINPFGREQSDLPLDEILDYNLEVSASVAPGDDLYFPEYLSDTFEDGSGDRFKIFESFRSALPRLNMISGGNDKAGNEKAVEMQI 433
[Close]
BLAST of Bestrophin homolog vs. Planmine SMEST
Match: SMESG000016290.1 (SMESG000016290.1)

HSP 1 Score: 403.29 bits (1035), Expect = 1.031e-138
Identity = 209/366 (57.10%), Postives = 269/366 (73.50%), Query Frame = 3 
Query:  198 LLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKE-NKSFELL--NKNLPKIS----IRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDD 1274
            +LYR+YLI DP KK  FESIV Y+NK A +VPLSFLLGFFVSTVLGR+W IVNSIPWM+KPAF CQA++  D E   + +RLTI+RY+NL+W+L++M LS KV++RF I    NK    L  + NL  IS    +R+++ K+NSDE+VK++FGKLIME+E+ V E ++     P F  PLMW T+L+EKA +  +IKS   C ++ NM+QD K+NL NLAV N FNVPLVYTQVIIIVVYTFLISE+ S+Q I+N NI I SYFPIF+IFKF IYMGWFK+GL+FINPFGSD +DLPLDE+LDYNL+IS+ IA   D  +PEYL  + ++   +  + LEKF     E   N + +++  +K Y D
Sbjct:    1 MLYRMYLIHDPNKKEAFESIVIYLNKVAGIVPLSFLLGFFVSTVLGRWWKIVNSIPWMTKPAFTCQALITEDTEPLCKDIRLTIVRYMNLAWILSVMYLSTKVKQRFIITSTTNKIISCLKDSPNLNTISKGKNVRKKMEKINSDEIVKKHFGKLIMEDEIRVFENVEHMSNKPSFITPLMWTTRLIEKAHRDGFIKSQGVCFNLFNMVQDFKSNLSNLAVHNEFNVPLVYTQVIIIVVYTFLISELLSEQIISNENILIGSYFPIFAIFKFIIYMGWFKMGLSFINPFGSDPSDLPLDEILDYNLEISSTIASGEDFYYPEYLTKTFKDPEDSPWKQLEKFRLLVPE-SFNVKKEEKCCSKTYVD 365
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BLAST of Bestrophin homolog vs. Planmine SMEST
Match: SMESG000016290.1 (SMESG000016290.1)

HSP 1 Score: 396.356 bits (1017), Expect = 5.486e-136
Identity = 206/362 (56.91%), Postives = 265/362 (73.20%), Query Frame = 3 
Query:  210 LYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWTIVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLSDKVRKRFWIKKE-NKSFELL--NKNLPKIS----IRERLNKVNSDEMVKRYFGKLIMEEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVNMIQDMKNNLDNLAVFNTFNVPLVYTQXXXXXXYTFLISEIFSQQTITNPNIDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQRFGNKCYDD 1274
            +YLI DP KK  FESIV Y+NK A +VPLSFLLGFFVSTVLGR+W IVNSIPWM+KPAF CQA++  D E   + +RLTI+RY+NL+W+L++M LS KV++RF I    NK    L  + NL  IS    +R+++ K+NSDE+VK++FGKLIME+E+ V E ++     P F  PLMW T+L+EKA +  +IKS   C ++ NM+QD K+NL NLAV N FNVPLVYTQVIIIVVYTFLISE+ S+Q I+N NI I SYFPIF+IFKF IYMGWFK+GL+FINPFGSD +DLPLDE+LDYNL+IS+ IA   D  +PEYL  + ++   +  + LEKF     E   N + +++  +K Y D
Sbjct:    1 MYLIHDPNKKEAFESIVIYLNKVAGIVPLSFLLGFFVSTVLGRWWKIVNSIPWMTKPAFTCQALITEDTEPLCKDIRLTIVRYMNLAWILSVMYLSTKVKQRFIITSTTNKIISCLKDSPNLNTISKGKNVRKKMEKINSDEIVKKHFGKLIMEDEIRVFENVEHMSNKPSFITPLMWTTRLIEKAHRDGFIKSQGVCFNLFNMVQDFKSNLSNLAVHNEFNVPLVYTQVIIIVVYTFLISELLSEQIISNENILIGSYFPIFAIFKFIIYMGWFKMGLSFINPFGSDPSDLPLDEILDYNLEISSTIASGEDFYYPEYLTKTFKDPEDSPWKQLEKFRLLVPE-SFNVKKEEKCCSKTYVD 361
The following BLAST results are available for this feature:
BLAST of Bestrophin homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BEST33.679e-4730.08bestrophin 3 [Source:HGNC Symbol;Acc:HGNC:17105][more]
BEST38.006e-4529.82bestrophin 3 [Source:HGNC Symbol;Acc:HGNC:17105][more]
BEST13.677e-4430.51bestrophin 1 [Source:HGNC Symbol;Acc:HGNC:12703][more]
BEST44.679e-4429.70bestrophin 4 [Source:HGNC Symbol;Acc:HGNC:17106][more]
BEST26.796e-4128.98bestrophin 2 [Source:HGNC Symbol;Acc:HGNC:17107][more]
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BLAST of Bestrophin homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
best-221.259e-4131.32Bestrophin homolog 22 [Source:UniProtKB/Swiss-Pro... [more]
best-221.424e-4131.61Bestrophin homolog 22 [Source:UniProtKB/Swiss-Pro... [more]
best-221.575e-4131.32Bestrophin homolog 22 [Source:UniProtKB/Swiss-Pro... [more]
best-221.947e-4131.32Bestrophin homolog 22 [Source:UniProtKB/Swiss-Pro... [more]
best-222.038e-4131.32Bestrophin homolog 22 [Source:UniProtKB/Swiss-Pro... [more]
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BLAST of Bestrophin homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Best47.194e-6032.15gene:FBgn0036491 transcript:FBtr0113172[more]
Best11.078e-5833.71gene:FBgn0040238 transcript:FBtr0082183[more]
Best11.633e-5833.71gene:FBgn0040238 transcript:FBtr0337020[more]
Best12.345e-5833.71gene:FBgn0040238 transcript:FBtr0114590[more]
Best13.215e-5833.71gene:FBgn0040238 transcript:FBtr0306023[more]
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BLAST of Bestrophin homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
best44.372e-5132.67bestrophin 4 [Source:ZFIN;Acc:ZDB-GENE-070912-6][more]
best45.346e-5132.05bestrophin 4 [Source:ZFIN;Acc:ZDB-GENE-070912-6][more]
best24.562e-4933.33bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127][more]
best12.352e-4830.68bestrophin 1 [Source:ZFIN;Acc:ZDB-GENE-110411-214][more]
best25.233e-2731.15bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127][more]
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BLAST of Bestrophin homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
best13.733e-4830.68bestrophin 1 [Source:NCBI gene;Acc:100302038][more]
best14.338e-4830.68bestrophin 1 [Source:NCBI gene;Acc:100302038][more]
best19.809e-4829.38bestrophin 1 [Source:NCBI gene;Acc:100302038][more]
best11.003e-4730.68bestrophin 1 [Source:NCBI gene;Acc:100302038][more]
ifitm31.569e-4631.82interferon induced transmembrane protein 3 [Source... [more]
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BLAST of Bestrophin homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Best17.088e-4530.31bestrophin 1 [Source:MGI Symbol;Acc:MGI:1346332][more]
Best17.153e-4430.03bestrophin 1 [Source:MGI Symbol;Acc:MGI:1346332][more]
Best39.805e-4429.57bestrophin 3 [Source:MGI Symbol;Acc:MGI:3580298][more]
Best21.599e-4029.18bestrophin 2 [Source:MGI Symbol;Acc:MGI:2387588][more]
Best21.599e-4029.18bestrophin 2 [Source:MGI Symbol;Acc:MGI:2387588][more]
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BLAST of Bestrophin homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8N1M1|BEST3_HUMAN3.845e-4429.82Bestrophin-3 OS=Homo sapiens OX=9606 GN=BEST3 PE=1... [more]
sp|O76090|BEST1_HUMAN1.766e-4330.51Bestrophin-1 OS=Homo sapiens OX=9606 GN=BEST1 PE=1... [more]
sp|Q8NFU0|BEST4_HUMAN2.247e-4329.70Bestrophin-4 OS=Homo sapiens OX=9606 GN=BEST4 PE=2... [more]
sp|O88870|BEST1_MOUSE5.003e-4330.03Bestrophin-1 OS=Mus musculus OX=10090 GN=Best1 PE=... [more]
sp|Q6UY87|BEST1_MACFA5.871e-4330.23Bestrophin-1 OS=Macaca fascicularis OX=9541 GN=BES... [more]
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BLAST of Bestrophin homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FRV12.218e-9036.87Bestrophin homolog OS=Macrostomum lignano OX=28230... [more]
A0A267GVJ04.641e-9036.87Bestrophin homolog OS=Macrostomum lignano OX=28230... [more]
A0A267FSL41.068e-8637.23Bestrophin homolog OS=Macrostomum lignano OX=28230... [more]
A0A267GVI21.985e-8637.23Bestrophin homolog OS=Macrostomum lignano OX=28230... [more]
A0A267FBA93.202e-8636.10Bestrophin homolog OS=Macrostomum lignano OX=28230... [more]
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BLAST of Bestrophin homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
best12.820e-5133.14bestrophin-2 [Source:NCBI gene;Acc:103045999][more]
best21.864e-4832.95bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127][more]
best41.047e-4530.23bestrophin 4 [Source:ZFIN;Acc:ZDB-GENE-070912-6][more]
best16.888e-4431.81bestrophin-2 [Source:NCBI gene;Acc:103045999][more]
BEST37.514e-2128.81bestrophin 3 [Source:HGNC Symbol;Acc:HGNC:17105][more]
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BLAST of Bestrophin homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
best19.887e-5030.77bestrophin 1 [Source:ZFIN;Acc:ZDB-GENE-110411-214][more]
best22.390e-4629.83bestrophin 2 [Source:ZFIN;Acc:ZDB-GENE-090312-127][more]
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BLAST of Bestrophin homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Bestrophin homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Bestrophin homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
BEST31.212e-5030.47bestrophin 3 [Source:NCBI gene;Acc:101172121][more]
best22.481e-4832.10bestrophin 2 [Source:NCBI gene;Acc:110015962][more]
best14.035e-4430.68bestrophin-2 [Source:NCBI gene;Acc:101167866][more]
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BLAST of Bestrophin homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000016291.10.000e+099.76SMESG000016291.1[more]
SMESG000016290.15.968e-16458.44SMESG000016290.1[more]
SMESG000016298.17.998e-16054.27SMESG000016298.1[more]
SMESG000016290.11.031e-13857.10SMESG000016290.1[more]
SMESG000016290.15.486e-13656.91SMESG000016290.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018184 ID=SMED30018184|Name=Bestrophin homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=1369bp
CTTCCGATCTATTTAATTTCTAATGATTGCTCATCATTTTTCCAGAAATG
CAATTGAAATCTGTGAAATATTTATCAGCAGTCTCGCTGAATGAAAGAGT
GACATTTCTGAAATTGCTGTTCCGTTGGCAATTTAGTGTATGGAAATTAT
TGTGGGCGGATTTTTTGTGGTTTTTTCTCATCTACACCGGGATCACTCTC
CTCTATCGACTATATCTCATTAAAGATCCTCAGAAAAAGGTCGTTTTTGA
ATCGATTGTGGCTTACATGAATAAGGCGGCAGCTCTCGTACCTTTGTCAT
TTCTGCTCGGCTTTTTCGTGTCAACAGTTTTGGGTCGATTTTGGACAATA
GTCAACTCGATTCCCTGGATGAGCAAGCCGGCATTCCTCTGTCAAGCGGT
ATTGAATGGAGACAACGAAGTGAAATCCAGACAGCTCCGGTTGACGATCA
TTCGATATCTGAACCTTTCATGGGTGCTGACCATGATGAAGCTGAGCGAC
AAAGTTAGAAAGCGGTTCTGGATAAAGAAGGAAAACAAATCTTTCGAGTT
ACTCAACAAAAATCTCCCAAAAATCTCCATCCGTGAGAGGCTTAACAAGG
TAAATTCTGATGAAATGGTCAAGAGGTATTTCGGGAAGTTGATCATGGAA
GAAGAGATGACAGTGTTGGAAGGATTAGATGATAAGCCGACAACTCCACA
GTTTTTCATACCCCTGATGTGGGCGACAAAGTTGGTGGAGAAAGCTAGGA
AGGCCGATTACATCAAGTCAGACAGCACTTGCTCTAGTATGGTCAACATG
ATTCAGGACATGAAGAACAATTTGGACAATTTGGCAGTTTTCAATACATT
CAATGTTCCGCTGGTTTACACCCAGGTGATCATCATTGTCGTGTACACCT
TTCTGATCAGTGAGATATTTTCCCAGCAAACAATCACTAACCCAAATATT
GACATTGAATCCTACTTTCCAATATTCAGTATATTCAAATTTTTCATTTA
CATGGGTTGGTTCAAGGTTGGCCTAACCTTTATCAACCCTTTTGGTAGTG
ATGACACAGATTTGCCACTAGACGAAATGCTCGACTACAATTTACAGATC
TCTGCTGTGATTGCTCGAGCTAGCGATTCGTGCTTTCCAGAATATTTGAA
CAGCTCTTTGGAAAACTCAGTAGTAAACAAGCTTGAAGTGCTTGAAAAAT
TTGAAGCAGAAAGATCAGAAATACAAACGAATTACAGAGATCAACAGCGT
TTTGGCAACAAATGTTATGACGACATGTTTATCAAATCTTAATTGTTGGT
AAACAATATTATTTTTTTTCTTTTTGCTGAGACGATAATATTAATAGCTT
ATAGTTGTTTAAAAAAAAA
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protein sequence of SMED30018184-orf-1

>SMED30018184-orf-1 ID=SMED30018184-orf-1|Name=SMED30018184-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=415bp
MQLKSVKYLSAVSLNERVTFLKLLFRWQFSVWKLLWADFLWFFLIYTGIT
LLYRLYLIKDPQKKVVFESIVAYMNKAAALVPLSFLLGFFVSTVLGRFWT
IVNSIPWMSKPAFLCQAVLNGDNEVKSRQLRLTIIRYLNLSWVLTMMKLS
DKVRKRFWIKKENKSFELLNKNLPKISIRERLNKVNSDEMVKRYFGKLIM
EEEMTVLEGLDDKPTTPQFFIPLMWATKLVEKARKADYIKSDSTCSSMVN
MIQDMKNNLDNLAVFNTFNVPLVYTQVIIIVVYTFLISEIFSQQTITNPN
IDIESYFPIFSIFKFFIYMGWFKVGLTFINPFGSDDTDLPLDEMLDYNLQ
ISAVIARASDSCFPEYLNSSLENSVVNKLEVLEKFEAERSEIQTNYRDQQ
RFGNKCYDDMFIKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000615Bestrophin
IPR021134Bestrophin/UPF0187
Vocabulary: molecular function
TermDefinition
GO:0005254chloride channel activity
Vocabulary: cellular component
TermDefinition
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0034707chloride channel complex
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006811ion transport
GO:0006821chloride transport
GO:1902476chloride transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021134Bestrophin/UPF0187PFAMPF01062Bestrophincoord: 18..350
e-value: 3.0E-68
score: 230.4
IPR000615BestrophinPANTHERPTHR10736BESTROPHINcoord: 6..369
NoneNo IPR availablePANTHERPTHR10736:SF55BESTROPHIN 1, ISOFORM C-RELATEDcoord: 6..369
NoneNo IPR availableTMHMMTMhelixcoord: 79..101
NoneNo IPR availableTMHMMTMhelixcoord: 270..292
NoneNo IPR availableTMHMMTMhelixcoord: 305..322
NoneNo IPR availableTMHMMTMhelixcoord: 39..58