SMED30018140

Overview
NameSMED30018140
Smed IDSMED30018140
Length (bp)1347
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018140

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30018140SMESG000062853.1 GCR127smed_ncbi_20200123PMID:27163480
Saberi et al., 2016		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30018140 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.014102:4648..5918 -6320
Homology
[Close]
BLAST of SMED30018140 vs. TrEMBL
Match: A0A193KUT4 (GCR127 OS=Schmidtea mediterranea OX=79327 GN=gcr127 PE=2 SV=1)

HSP 1 Score: 725.702 bits (1872), Expect = 0.000e+0
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = -1 
Query:  133 MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWSDDASF 1209
            MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWSDDASF
Sbjct:    1 MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWSDDASF 359
[Close]
BLAST of SMED30018140 vs. TrEMBL
Match: A0A193KUD6 (GCR130 OS=Schmidtea mediterranea OX=79327 GN=gcr130 PE=2 SV=1)

HSP 1 Score: 400.208 bits (1027), Expect = 2.643e-134
Identity = 193/358 (53.91%), Postives = 260/358 (72.63%), Query Frame = -1 
Query:  133 EEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYAN-YETASSIFNALF--QKTTKFSATLSKGSSTAKRWSDDASF 1197
            + P  L+ +EI++M IGM GNI CC+LM R+RLMPD+NVN+NAV +L+QS LDFF  LFYI+SL+ YPKI ++ LG FVCR+I+S+ALFWMI +LRA +N+VI I NFIKLLYPFL +  NLI F+  GL +L  VSL+Q++P++  VSF  N    NCNFSAI  F E T L G++R +A+FNFIYLY+FP+T T+F+YKQ+++CLK+S+D  RKI  +EIMKSFL+DTV Y  CFS HSI+ FL +F+    Y +T   YRIGM ++ +YAV+YPYISM TRR Y+KP+SD+ K+ G K     + K+S SKY +  + + S F+  F  ++  K S   SK +S+   WSD+ SF
Sbjct:    4 DVPPPLHPVEIALMAIGMVGNILCCMLMFRNRLMPDKNVNLNAVLNLLQSLLDFFSNLFYIISLISYPKIKSEALGAFVCRVIRSQALFWMISTLRAGVNVVIAILNFIKLLYPFLSTSGNLIGFIGIGLALLFVVSLIQVVPHVSTVSFSPNSTKHNCNFSAILNFQEVTGLVGMVRFFALFNFIYLYIFPLTATMFLYKQVFNCLKESDDEPRKIAYREIMKSFLMDTVLYLLCFSMHSILFFLYQFSSKIGYLVTSTLYRIGMLLVALYAVLYPYISMATRRAYRKPISDVMKIFGFKQYNTTIYKQSISKYGHSIDNSGSRFSKFFSSKQKNKPSGNTSKPTSSVNDWSDNDSF 361
[Close]
BLAST of SMED30018140 vs. TrEMBL
Match: A0A504Y807 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_07433 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.924e-22
Identity = 72/296 (24.32%), Postives = 154/296 (52.03%), Query Frame = -1 
Query:  184 SLVQSALDFFLCLFYILSLMDYPK-IHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTV-SLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSI-IMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSAT 1062
             +VQ+ +D+ +C  +++ L      + ++F+ +F+C +I S AL W + ++R  +N++   F FI+ ++PF++ +      +   +G+L+++  LL I+P+  +VS+   D +  C+      F+  ++   +LRL  +   +Y +L P+   +++Y+ +   L+  + +      KE+  + +LD V+    FSF ++ IMFL ++ +     I+       + ++ +Y+V+YP  S++ RR Y+KPM D    +G+      L +R +         S I     ++TTK + +
Sbjct:   51 GVVQTVIDWLICTVFVIELCSIQVFVQHNFIAMFLCHLISSDALLWGLIAIRWNMNVLTAFFYFIQFIFPFMR-IEQQKKSMNLAMGILLSLCGLLGIVPHAFIVSY--RDDISACSRDVGEEFLVPSRTQIVLRLVILLGVVYNFLVPLGTGMYLYRHVLRVLRVRDKSQNAAAYKEMRLNAILD-VWLQGAFSFPAVCIMFLQKYPNADWLKISSNVQFAVVFLLCVYSVIYPGASVLFRRKYRKPMLDCLIQMGMHPTDSWLAQRWSLAAGERGKTSYISLNSRRRTTKIARS 342
[Close]
BLAST of SMED30018140 vs. TrEMBL
Match: A0A4E0R9Z6 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_006399 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.114e-21
Identity = 71/296 (23.99%), Postives = 152/296 (51.35%), Query Frame = -1 
Query:  184 SLVQSALDFFLCLFYILSLMDYPK-IHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTV-SLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSI-IMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSAT 1062
             +VQ+ +D+ +C+ +++ L      +   F+ + +C +I S AL W + ++R  +N++   F FI+ ++PF++ +      +   +G+L+++  LL I+P+  +VS+   D +  C+      F+  ++   +LRL  +   +Y +L P+   +++Y+ +   L+  + +      KE+  + +LD V+    FSF ++ IMFL ++ +     I+       + ++ +Y+V+YP  S++ RR Y+KPM D    +G+      L +R +         S I     ++TTK + +
Sbjct:   51 GVVQTVIDWLICIVFVIGLCSIQVFVQQKFIAMILCHLISSDALLWGLIAIRWNMNVLTAFFYFIQFVFPFMR-IEQQKKSMNLAMGILLSLCGLLGIVPHAFIVSY--RDDISACSRDVGEEFLVPSRTQIVLRLVILLGVVYNFLVPLGTGMYLYRHVLRVLRVRDKSQNAAAYKEMRLNAILD-VWLQAAFSFPAVCIMFLQKYPNADWLKISSNVQFAVIFLLCVYSVIYPGASVLFRRKYRKPMLDCLIQMGMHPTDSWLAQRWSLAAGERGKTSYISVNSRRRTTKLARS 342
[Close]
BLAST of SMED30018140 vs. TrEMBL
Match: A0A4S2LBB4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008559 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.254e-18
Identity = 83/321 (25.86%), Postives = 142/321 (44.24%), Query Frame = -1 
Query:  241 YGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVS-LLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSK----YANYE 1188
            Y +    I +  +G   NI   I +SR+               +VQ ++D   C+ Y+LS +D   +   F  IF CRI++++ALFW   + RA IN+   ++  ++++YPF  +V N       GL +L T + +LQ +     +S +  D+   C      +  E   ++  L LY+    IY + FP       +  +   L D +    + + KE  ++  +D V +  C S H  +  L+ F  N EY +    Y +   + + Y V+ P  SM  R+    P+     L  V  P   +D +  SK    ++NYE
Sbjct:    3 YAITVTGILLATVGTVINIISLIFVSRTTCQAGTLAKKVLYIIIVQHSIDIVACIAYLLSSVD---LDEQFPSIFTCRILRTRALFWAAINYRAYINVYGSLWQHVRIIYPFWSTVLNRTEG-CVGLFLLATAANILQTLA-FTRLSELSADQ-STCIPCTPCQTGENAGIYSFLVLYST---IYRFAFPAMAMFASFGYLMQVLSDCDGKVYRQMYKETRRNLFIDAVLFVICLSTHEALFTLNVFASNKEYGLRTAPYEVASLLALGYPVLSPCFSMCLRKTNYTPV-----LEAVLRP---MDYQKLSKLAIMWSNYE 306
[Close]
BLAST of SMED30018140 vs. Planmine SMEST
Match: SMESG000062853.1 (SMESG000062853.1)

HSP 1 Score: 725.702 bits (1872), Expect = 0.000e+0
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = -1 
Query:  133 MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWSDDASF 1209
            MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWSDDASF
Sbjct:    1 MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWSDDASF 359
[Close]
BLAST of SMED30018140 vs. Planmine SMEST
Match: SMESG000062854.1 (SMESG000062854.1)

HSP 1 Score: 400.208 bits (1027), Expect = 7.350e-138
Identity = 193/358 (53.91%), Postives = 260/358 (72.63%), Query Frame = -1 
Query:  133 EEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYAN-YETASSIFNALF--QKTTKFSATLSKGSSTAKRWSDDASF 1197
            + P  L+ +EI++M IGM GNI CC+LM R+RLMPD+NVN+NAV +L+QS LDFF  LFYI+SL+ YPKI ++ LG FVCR+I+S+ALFWMI +LRA +N+VI I NFIKLLYPFL +  NLI F+  GL +L  VSL+Q++P++  VSF  N    NCNFSAI  F E T L G++R +A+FNFIYLY+FP+T T+F+YKQ+++CLK+S+D  RKI  +EIMKSFL+DTV Y  CFS HSI+ FL +F+    Y +T   YRIGM ++ +YAV+YPYISM TRR Y+KP+SD+ K+ G K     + K+S SKY +  + + S F+  F  ++  K S   SK +S+   WSD+ SF
Sbjct:    4 DVPPPLHPVEIALMAIGMVGNILCCMLMFRNRLMPDKNVNLNAVLNLLQSLLDFFSNLFYIISLISYPKIKSEALGAFVCRVIRSQALFWMISTLRAGVNVVIAILNFIKLLYPFLSTSGNLIGFIGIGLALLFVVSLIQVVPHVSTVSFSPNSTKHNCNFSAILNFQEVTGLVGMVRFFALFNFIYLYIFPLTATMFLYKQVFNCLKESDDEPRKIAYREIMKSFLMDTVLYLLCFSMHSILFFLYQFSSKIGYLVTSTLYRIGMLLVALYAVLYPYISMATRRAYRKPISDVMKIFGFKQYNTTIYKQSISKYGHSIDNSGSRFSKFFSSKQKNKPSGNTSKPTSSVNDWSDNDSF 361
[Close]
BLAST of SMED30018140 vs. Planmine SMEST
Match: SMESG000062839.1 (SMESG000062839.1)

HSP 1 Score: 131.339 bits (329), Expect = 3.262e-34
Identity = 84/280 (30.00%), Postives = 147/280 (52.50%), Query Frame = -1 
Query:  316 ILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRN-----DKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYF------FCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDI 1122
            I+M + + +   + N++ + S VQ+ LD  +CL YIL    YP+ + +   I +C+ + + AL W + + R+ + +VI + NFIKLLYPF+    N+ +    G+ +   +S + +IP +   +   N     D    CNF  I + +  T   G+  +Y+++ F+YLYLFPI     +Y QI DCL    D++R    +E++K + LD VF F      +CF   S   F +++N    Y      +R+ + +IV+Y+  Y  +S+I +    K  +D+
Sbjct:   23 IIMIKYKPVNYSHFNLSILMSKVQAILDIVICLLYILRFTKYPERNGNLSKIIICKYVVNLALLWFVVTFRSLLKVVICLNNFIKLLYPFMNVRGNVEIVFVVGIIINFAISCVAVIPYVISSAPNENVELNVDIESYCNFKKIEKIINETDFIGLQYIYSIYQFVYLYLFPIIACYILYNQILDCLCQHVDSTRMQAYREVLKCYWLD-VFLFAILYGPYCFYSLS-YCFSAQYNSRIYYD----DHRVYIFLIVVYSCSYSVLSLIFKSSNVKARNDL 296
[Close]
BLAST of SMED30018140 vs. Planmine SMEST
Match: SMESG000062838.1 (SMESG000062838.1)

HSP 1 Score: 118.627 bits (296), Expect = 9.110e-30
Identity = 79/329 (24.01%), Postives = 165/329 (50.15%), Query Frame = -1 
Query:  148 MSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNI------GMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAKLLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTAKRWS 1116
            M R      R+ N++   + +QS +D  +C+ Y+L  ++YP  +     + +C+ I + A FW + S R+ +N+VI + NF+KLLYPF+            GL + + +S + I+P +        V+   N +   C+F  I + ++ +   G+  +Y++++F+YLYLFPI     +Y QI +CL  + D +R ++ +E++K + LDT  +   +  + +      F+  +E  I     ++ + +IV+Y+  Y  +S++ +    K  +++ ++L  +   ++ + +    Y  Y    +    L + +   S + ++ S +   WS
Sbjct:    1 MIRGTSAHHRHFNVSLFMNKLQSVMDILICVLYLLRAINYPLNNGKISRVIICKFIMNLAFFWFVVSFRSLLNVVICLNNFVKLLYPFMNIRGTFESIFLVGLLINIIISFISIVPYVLASGPNEQVTVSVNTE-SYCHFDEITKVIQDSDFIGLQYIYSIYHFVYLYLFPIIACYILYNQILNCLCQNVDKTRILIYQELLKCYWLDTYLFAILYGPYCLYSLSFCFSSQYEARIYFNDNQMYIFLIVVYSCTYSTLSLLFKSSNVKARNEMLRMLPCE-IKVLQNMKGGYLYREYGLQKNRSKYLGKGSDSMSVSQTESSES---WS 324
[Close]
BLAST of SMED30018140 vs. Planmine SMEST
Match: SMESG000062805.1 (SMESG000062805.1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.468e-18
Identity = 73/278 (26.26%), Postives = 138/278 (49.64%), Query Frame = -1 
Query:  331 IGMSGNIACCILMSRSRLMPDRNVNMNAVFSLVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLRAAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIGMVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVTLFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFLSEFNDNFE----YSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKK 1152
            +G+  N+    L+  +R + D+ +N + +F+L  +  D  +CL ++++LMD    +  F+G  +C +  + + F+ + S R+ + +++ +++FIKLLYPFL +       +++G  +L+  S+L  IP I     IR   +    +   +  +E     G   L  +  F Y +L P   T+F Y  I   +   E  SR+    E+ KSFL+D++ +  C+   S+ +    F+ +FE      I D S       I MY++ Y   SMI +  +++
Sbjct:   10 LGVIINVIFFYLLLINRHLSDKTINKSILFNLAITLCDGLICLEFLINLMDKWMFYEGFVGKLMCILFYNDSAFYFLMSCRSGLTLLMCLYHFIKLLYPFLVNSVYFTFVVSSGFVLLLGNSIL--IPFI----IIRRTLIYGYEYELTYCLLEPFNYEGTQFLINIVFFTYSFLMPGIGTVFFYAHILKSINSIETISREKAFVELRKSFLIDSLLFIICYLHFSLKLLSYCFSVSFEKENHLDIRDSS----SITIGMYSMSYALFSMIFKSSHRR 277
The following BLAST results are available for this feature:
BLAST of SMED30018140 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUT40.000e+0100.00GCR127 OS=Schmidtea mediterranea OX=79327 GN=gcr12... [more]
A0A193KUD62.643e-13453.91GCR130 OS=Schmidtea mediterranea OX=79327 GN=gcr13... [more]
A0A504Y8072.924e-2224.32Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A4E0R9Z61.114e-2123.99Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A4S2LBB41.254e-1825.86Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of SMED30018140 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30018140 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062853.10.000e+0100.00SMESG000062853.1[more]
SMESG000062854.17.350e-13853.91SMESG000062854.1[more]
SMESG000062839.13.262e-3430.00SMESG000062839.1[more]
SMESG000062838.19.110e-3024.01SMESG000062838.1[more]
SMESG000062805.11.468e-1826.26SMESG000062805.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018140 ID=SMED30018140|Name=SMED30018140|organism=Schmidtea mediterranea sexual|type=transcript|length=1347bp
TTTTTTTTAAATAAAATCATTTTAATTTTTTTTTAATTGTTACAATCATG
TACTGATTTTAAAAATTCCTATTGTCAATATATTTAGATGGGTTTTTTGA
CTGTTAAATTATGGTTTTGATGCAATATTTTAAAAACTGGCATCATCTGA
CCATCGCTTTGCAGTAGACGAACCTTTTGACAGTGTAGCGCTAAACTTTG
TCGTTTTTTGAAATAGAGCGTTGAAAATAGACGAGGCTGTTTCGTAATTT
GCATATTTAGAGGTCGATCGTTTGTCAAGAATGATTGCTGGCGACTTGAC
ACCCAATAATTTAGCAATGTCGGACATAGGCTTTTTGTATGGTCTTCGTG
TAATCATGGAAATGTAAGGATAGACAACTGCATACATTACAATCATGGCC
ATGCCAATTCGATATGATTTATCTGTTATAGAATATTCAAAATTATCATT
GAACTCAGACAGGAACATGATTATACTGTGGAAAGAAAAGCAAAAGAAAT
AGAAAACAGTATCTAATAAGAAGCTTTTCATTATTTCTTTCATAACAATT
TTCCTCGATGTATCTTCGCTGTCTTTCAGACAATCATATATCTGTTTGTA
TACAAAAAGTGTAACTGTAATTGGAAAGAGATACAGATAAATGAAATTGA
AGACAGCATAAAGTCTTAGTATGCCAAAAAGTTTTGTAAATTCTACGAAT
CTGTGAATGGCTGAGAAATTACAATTTCGCATTTTATCATTTCTAATAAA
ACTAACCATTCCAATGTTTGGTATGATCTGTAATAATGAAACAGTGACCA
ACATCCCTAAACCAGCAGTTAGAAACATTATTAAATTGCTGACACTTTTT
AGAAAAGGATAAAGTAATTTTATGAAATTAAAAATCCCAATCACTATGTT
TATTGCGGCTCTTAAAGAGCATATCATCCAAAACAATGCTTTACTCTTTA
TTATCCGACAGACAAATATTCCGAGAAAATCGTTGTGGATTTTTGGATAA
TCCATTAGTGAAAGGATGTAAAATAAGCATAAAAAGAAGTCTAGAGCAGA
CTGCACCAAACTAAAAACTGCATTCATATTAACATTTCTGTCTGGCATCA
GTCGACTTCTTGACATTAAAATGCAACAGGCAATATTTCCTGACATTCCA
ATTACCATTATTGAGATTTCAATGGAATAGAGACCATAAGGTTCTTCTCG
AATTATCATTTTGGTATATTTCAATATACAATCAATAATTATTTATTTAA
TTCTTAATTAATTTTATTTTATATAATTCATGCCCTACCCGTTCACTTTA
TTGTCAAAACTCAAAAAACGCATTTTACAGGGTTTTGCATTATTATA
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protein sequence of SMED30018140-orf-1

>SMED30018140-orf-1 ID=SMED30018140-orf-1|Name=SMED30018140-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=360bp
MIIREEPYGLYSIEISIMVIGMSGNIACCILMSRSRLMPDRNVNMNAVFS
LVQSALDFFLCLFYILSLMDYPKIHNDFLGIFVCRIIKSKALFWMICSLR
AAINIVIGIFNFIKLLYPFLKSVSNLIMFLTAGLGMLVTVSLLQIIPNIG
MVSFIRNDKMRNCNFSAIHRFVEFTKLFGILRLYAVFNFIYLYLFPITVT
LFVYKQIYDCLKDSEDTSRKIVMKEIMKSFLLDTVFYFFCFSFHSIIMFL
SEFNDNFEYSITDKSYRIGMAMIVMYAVVYPYISMITRRPYKKPMSDIAK
LLGVKSPAIILDKRSTSKYANYETASSIFNALFQKTTKFSATLSKGSSTA
KRWSDDASF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
Vocabulary: INTERPRO
TermDefinition
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.462