MADS-box domain-containing protein

Overview


Name	MADS-box domain-containing protein
Smed ID	SMED30018122
Length (bp)	1295

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of MADS-box domain-containing protein (SMED30018122) t-SNE clustered cells

Violin plots show distribution of expression levels for MADS-box domain-containing protein (SMED30018122) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MADS-box domain-containing protein (SMED30018122) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MADS-box domain-containing protein (SMED30018122) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30018122

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30018122	SMESG000005612.1	Contig594	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30018122	SMESG000005612.1	Contig594	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30018122	SMESG000005612.1	Contig594	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30018122	SMESG000011575.1	PL04024A2A08	ncbi_smed_ests	PMID:23079596 Forsthoefel et al., 2012
pharynx	SMED30018122	SMESG000011575.1	Contig594	GPL15192	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30018122	SMESG000011575.1	Contig594	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30018122	SMESG000011575.1	Contig594	GPL15192	PMID:23079596 Forsthoefel et al., 2012
epidermis	SMED30018122	SMESG000011575.1	dd_Smed_v4_6536_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30018122	SMESG000011575.1	dd_Smed_v4_6536_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30018122	SMESG000011575.1	dd_Smed_v4_6536_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30018122	SMESG000011575.1	dd_Smed_v4_6536_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Human
Match: MEF2A (myocyte enhancer factor 2A [Source:HGNC Symbol;Acc:HGNC:6993])

HSP 1 Score: 56.225 bits (134), Expect = 9.608e-10
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Human
Match: MEF2C (myocyte enhancer factor 2C [Source:HGNC Symbol;Acc:HGNC:6996])

HSP 1 Score: 56.6102 bits (135), Expect = 1.126e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Human
Match: MEF2A (myocyte enhancer factor 2A [Source:HGNC Symbol;Acc:HGNC:6993])

HSP 1 Score: 56.225 bits (134), Expect = 1.392e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Human
Match: MEF2C (myocyte enhancer factor 2C [Source:HGNC Symbol;Acc:HGNC:6996])

HSP 1 Score: 56.6102 bits (135), Expect = 2.136e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Human
Match: MEF2D (myocyte enhancer factor 2D [Source:HGNC Symbol;Acc:HGNC:6997])

HSP 1 Score: 56.225 bits (134), Expect = 4.349e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Celegans
Match: mef-2 (Related to vertebrate MEF2 transcription factor [Source:UniProtKB/TrEMBL;Acc:Q9U325])

HSP 1 Score: 58.9214 bits (141), Expect = 2.594e-9
Identity = 26/49 (53.06%), Postives = 34/49 (69.39%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I+D+R R  TF KR+ GL KKA EL+ LC   I++I+ N
Sbjct:    1 MGRKKIQITRIQDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIVFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Celegans
Match: unc-120 (pep chromosome:WBcel235:I:8460225:8463015:1 gene:WBGene00006844.1 transcript:D1081.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:unc-120)

HSP 1 Score: 48.521 bits (114), Expect = 4.789e-6
Identity = 22/59 (37.29%), Postives = 37/59 (62.71%), Query Frame = 2

Query:  110 GRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDL-VYKFSDP 283
            GR KI M +I +  +R +TF KR+ G+ KKA EL+ L G  + +++ ++   VY ++ P
Sbjct:   46 GRVKIKMEYINNKLRRYTTFSKRKTGIMKKAFELSTLTGTQVMLLVASETGHVYTYATP 104

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Fly
Match: Mef2 (gene:FBgn0011656 transcript:FBtr0088446)

HSP 1 Score: 56.9954 bits (136), Expect = 2.089e-8
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVII 247
            MGRKKI ++ I D+R R  TF+KR+ G+ KKA EL+ LC   I++II
Sbjct:    1 MGRKKIQISRITDERNRQVTFNKRKFGVMKKAYELSVLCDCEIALII 47

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Fly
Match: Mef2 (gene:FBgn0011656 transcript:FBtr0088444)

HSP 1 Score: 56.6102 bits (135), Expect = 2.262e-8
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVII 247
            MGRKKI ++ I D+R R  TF+KR+ G+ KKA EL+ LC   I++II
Sbjct:    1 MGRKKIQISRITDERNRQVTFNKRKFGVMKKAYELSVLCDCEIALII 47

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Fly
Match: Mef2 (gene:FBgn0011656 transcript:FBtr0088443)

HSP 1 Score: 56.6102 bits (135), Expect = 2.349e-8
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVII 247
            MGRKKI ++ I D+R R  TF+KR+ G+ KKA EL+ LC   I++II
Sbjct:    1 MGRKKIQISRITDERNRQVTFNKRKFGVMKKAYELSVLCDCEIALII 47

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Fly
Match: Mef2 (gene:FBgn0011656 transcript:FBtr0088445)

HSP 1 Score: 56.6102 bits (135), Expect = 2.382e-8
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVII 247
            MGRKKI ++ I D+R R  TF+KR+ G+ KKA EL+ LC   I++II
Sbjct:    1 MGRKKIQISRITDERNRQVTFNKRKFGVMKKAYELSVLCDCEIALII 47

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Fly
Match: Mef2 (gene:FBgn0011656 transcript:FBtr0088447)

HSP 1 Score: 56.6102 bits (135), Expect = 2.442e-8
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVII 247
            MGRKKI ++ I D+R R  TF+KR+ G+ KKA EL+ LC   I++II
Sbjct:    1 MGRKKIQISRITDERNRQVTFNKRKFGVMKKAYELSVLCDCEIALII 47

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Zebrafish
Match: mef2d (myocyte enhancer factor 2d [Source:ZFIN;Acc:ZDB-GENE-990415-164])

HSP 1 Score: 56.9954 bits (136), Expect = 3.875e-10
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Zebrafish
Match: mef2cb (myocyte enhancer factor 2cb [Source:ZFIN;Acc:ZDB-GENE-040901-7])

HSP 1 Score: 56.225 bits (134), Expect = 6.642e-10
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Zebrafish
Match: mef2ca (myocyte enhancer factor 2ca [Source:ZFIN;Acc:ZDB-GENE-980526-253])

HSP 1 Score: 55.8398 bits (133), Expect = 1.126e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIARIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIARIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIARIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
Match: mef2a (myocyte enhancer factor 2A [Source:NCBI gene;Acc:100216045])

HSP 1 Score: 56.225 bits (134), Expect = 4.535e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
Match: mef2a (myocyte enhancer factor 2A [Source:NCBI gene;Acc:100216045])

HSP 1 Score: 56.6102 bits (135), Expect = 1.069e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
Match: mef2c (MADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C) [Source:NCBI gene;Acc:100127198])

HSP 1 Score: 57.3806 bits (137), Expect = 1.938e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
Match: mef2d (myocyte enhancer factor 2D [Source:NCBI gene;Acc:780389])

HSP 1 Score: 57.7658 bits (138), Expect = 1.989e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
Match: mef2c (MADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C) [Source:NCBI gene;Acc:100127198])

HSP 1 Score: 57.7658 bits (138), Expect = 1.999e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
Match: Mef2c (myocyte enhancer factor 2C [Source:MGI Symbol;Acc:MGI:99458])

HSP 1 Score: 56.6102 bits (135), Expect = 1.041e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
Match: Mef2c (myocyte enhancer factor 2C [Source:MGI Symbol;Acc:MGI:99458])

HSP 1 Score: 56.6102 bits (135), Expect = 1.331e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
Match: Mef2a (myocyte enhancer factor 2A [Source:MGI Symbol;Acc:MGI:99532])

HSP 1 Score: 55.8398 bits (133), Expect = 2.038e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
Match: Mef2c (myocyte enhancer factor 2C [Source:MGI Symbol;Acc:MGI:99458])

HSP 1 Score: 55.8398 bits (133), Expect = 3.156e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
Match: Mef2c (myocyte enhancer factor 2C [Source:MGI Symbol;Acc:MGI:99458])

HSP 1 Score: 55.8398 bits (133), Expect = 3.541e-9
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
Match: sp|Q12224|RLM1_YEAST (Transcription factor RLM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RLM1 PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.731e-10
Identity = 33/58 (56.90%), Postives = 41/58 (70.69%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK-DLVYKFS 277
            MGR+KI +  I DDR RA TF KR+ GLFKKA EL+ LC V I+VII+   +  Y+FS
Sbjct:    1 MGRRKIEIQRISDDRNRAVTFIKRKAGLFKKAHELSVLCQVDIAVIILGSNNTFYEFS 58

[Close]

BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
Match: sp|P38128|SMP1_YEAST (Transcription factor SMP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SMP1 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.275e-9
Identity = 31/58 (53.45%), Postives = 41/58 (70.69%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK-DLVYKFS 277
            MGR+KI +  I+DDR R  TF KR+ GLFKKA EL+ LC V I+VII+   +  Y++S
Sbjct:    1 MGRRKIEIEPIKDDRNRTVTFIKRKAGLFKKAHELSVLCQVDIAVIILGSNNTFYEYS 58

[Close]

BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
Match: sp|Q4PSU4|AGL61_ARATH (Agamous-like MADS-box protein AGL61 OS=Arabidopsis thaliana OX=3702 GN=AGL61 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.198e-8
Identity = 30/64 (46.88%), Postives = 38/64 (59.38%), Query Frame = 2

Query:  104 AMGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSF 295
            ++GR+KI M  I+ +  R  TF KRR GLFKKASEL  LCG  I +I      V+  +  P SF
Sbjct:   61 SIGRQKIPMVKIKKESHRQVTFSKRRAGLFKKASELCTLCGAEIGII------VFSPAKKPFSF 118

[Close]

BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
Match: sp|O64703|AGL29_ARATH (Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 2.431e-8
Identity = 24/47 (51.06%), Postives = 31/47 (65.96%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVII 247
            MGR+KI M  ++D   R  TF KRR GLFKKASEL  LC   + +++
Sbjct:    1 MGRRKIKMEMVQDMNTRQVTFSKRRTGLFKKASELATLCNAELGIVV 47

[Close]

BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
Match: sp|Q54QY7|SRFD_DICDI (Serum factor response D OS=Dictyostelium discoideum OX=44689 GN=srfD PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.614e-8
Identity = 27/49 (55.10%), Postives = 35/49 (71.43%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF+KR+ GL KKA EL  LC  ++S++IVN
Sbjct:    1 MGRKKIKIQRIPDERNRQVTFNKRKNGLIKKAMELALLCDSTVSLVIVN 49

[Close]

BLAST of MADS-box domain-containing protein vs. TrEMBL
Match: S8DI54 (MADS-box domain-containing protein (Fragment) OS=Genlisea aurea OX=192259 GN=M569_15720 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.597e-10
Identity = 33/64 (51.56%), Postives = 44/64 (68.75%), Query Frame = 2

Query:  104 AMGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSF 295
            ++GR+KI M  IEDD  R  TF KRR+G+FKKA+EL+ LCGV I+VII      + F+   +SF
Sbjct:    8 SLGRRKIEMKLIEDDNARTVTFSKRRVGVFKKATELSILCGVQIAVII------FSFAGRAYSF 65

[Close]

BLAST of MADS-box domain-containing protein vs. TrEMBL
Match: A0A0G4IL56 (MADS-box domain-containing protein OS=Plasmodiophora brassicae OX=37360 GN=PBRA_004543 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.291e-10
Identity = 29/57 (50.88%), Postives = 42/57 (73.68%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFS 277
            MGR+KI +  I D + R  TF+KR++GL KKA EL+ LCGV +++++  KD +YKFS
Sbjct:    1 MGRRKIKIEMIPDAKNRTVTFNKRKVGLLKKAIELSVLCGVDVALVVFGKDTIYKFS 57

[Close]

BLAST of MADS-box domain-containing protein vs. TrEMBL
Match: A0A0H5RAW9 (MADS-box domain-containing protein OS=Spongospora subterranea OX=70186 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.078e-9
Identity = 30/57 (52.63%), Postives = 41/57 (71.93%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFS 277
            MGR+KI +  I D R R  TF+KR++GL KKA EL+ LCG  I+++I  ++ VYKFS
Sbjct:    1 MGRRKIKIEMIADKRNRTVTFNKRKLGLMKKAIELSVLCGTDIALVIFGQESVYKFS 57

[Close]

BLAST of MADS-box domain-containing protein vs. TrEMBL
Match: A0A016U7J8 (MADS-box domain-containing protein OS=Ancylostoma ceylanicum OX=53326 GN=Acey_s0053.g2316 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.723e-9
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 2

Query:   83 ILHKIIFAMGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            +L ++ F+MGRKKI +  I+D+R R  TF KR+ GL KKA EL+ LC   I++I+ N
Sbjct:   18 LLRRVTFSMGRKKIQITRIQDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIVFN 74

[Close]

BLAST of MADS-box domain-containing protein vs. TrEMBL
Match: A0A4P9ZI70 (SRF-TF-domain-containing protein (Fragment) OS=Metschnikowia bicuspidata OX=27322 GN=METBISCDRAFT_11366 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.676e-9
Identity = 33/58 (56.90%), Postives = 43/58 (74.14%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK-DLVYKFS 277
            MGR+KI +  +EDDR R+ TF KR+ GLFKKA EL+ LC V ++VIIV   + VY+FS
Sbjct:    1 MGRRKIEIEPLEDDRNRSVTFVKRKAGLFKKAYELSVLCEVDLAVIIVGSNNKVYEFS 58

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
Match: mef2d (myocyte enhancer factor 2D [Source:NCBI gene;Acc:103037494])

HSP 1 Score: 57.7658 bits (138), Expect = 1.291e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
Match: mef2d (myocyte enhancer factor 2D [Source:NCBI gene;Acc:103037494])

HSP 1 Score: 57.7658 bits (138), Expect = 1.299e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
Match: mef2d (myocyte enhancer factor 2D [Source:NCBI gene;Acc:103037494])

HSP 1 Score: 57.7658 bits (138), Expect = 1.332e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
Match: mef2d (myocyte enhancer factor 2D [Source:NCBI gene;Acc:103037494])

HSP 1 Score: 57.7658 bits (138), Expect = 1.366e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
Match: mef2d (myocyte enhancer factor 2D [Source:NCBI gene;Acc:103037494])

HSP 1 Score: 57.7658 bits (138), Expect = 1.416e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Yeast
Match: RLM1 (MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication [Source:SGD;Acc:S000006010])

HSP 1 Score: 66.6254 bits (161), Expect = 2.492e-12
Identity = 33/58 (56.90%), Postives = 41/58 (70.69%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK-DLVYKFS 277
            MGR+KI +  I DDR RA TF KR+ GLFKKA EL+ LC V I+VII+   +  Y+FS
Sbjct:    1 MGRRKIEIQRISDDRNRAVTFIKRKAGLFKKAHELSVLCQVDIAVIILGSNNTFYEFS 58

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Yeast
Match: SMP1 (MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication [Source:SGD;Acc:S000000386])

HSP 1 Score: 62.7734 bits (151), Expect = 3.277e-11
Identity = 31/58 (53.45%), Postives = 41/58 (70.69%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK-DLVYKFS 277
            MGR+KI +  I+DDR R  TF KR+ GLFKKA EL+ LC V I+VII+   +  Y++S
Sbjct:    1 MGRRKIEIEPIKDDRNRTVTFIKRKAGLFKKAHELSVLCQVDIAVIILGSNNTFYEYS 58

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Yeast
Match: MCM1 (Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000004646])

HSP 1 Score: 56.225 bits (134), Expect = 2.592e-9
Identity = 26/58 (44.83%), Postives = 39/58 (67.24%), Query Frame = 2

Query:  113 RKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK-DLVYKFSDP 283
            R+KI + FIE+  +R  TF KR+ G+ KKA EL+ L G  + +++V++  LVY FS P
Sbjct:   18 RRKIEIKFIENKTRRHVTFSKRKHGIMKKAFELSVLTGTQVLLLVVSETGLVYTFSTP 75

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Yeast
Match: ARG80 (Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p [Source:SGD;Acc:S000004645])

HSP 1 Score: 51.2174 bits (121), Expect = 3.763e-8
Identity = 26/58 (44.83%), Postives = 38/58 (65.52%), Query Frame = 2

Query:  113 RKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIV-NKDLVYKFSDP 283
            R+K  + +IE+  +R  TF KRR G+ KKA EL+ L G +I ++I+ N  LVY F+ P
Sbjct:   80 RRKQPIRYIENKTRRHVTFSKRRHGIMKKAYELSVLTGANILLLILANSGLVYTFTTP 137

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Nematostella
Match: MEF2 (MADS-box transcription factor; Myocyte enhancer factor 2 splice variant IV; Predicted protein [Source:UniProtKB/TrEMBL;Acc:Q6S5H1])

HSP 1 Score: 57.3806 bits (137), Expect = 1.332e-9
Identity = 27/49 (55.10%), Postives = 34/49 (69.39%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI ++ I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQISRIGDERNRQVTFTKRKFGLMKKAYELSILCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Nematostella
Match: EDO43028 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZP6])

HSP 1 Score: 48.9062 bits (115), Expect = 3.162e-7
Identity = 23/57 (40.35%), Postives = 36/57 (63.16%), Query Frame = 2

Query:  110 GRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDL-VYKFS 277
            GR KI M FI +  +R +TF KR+ G+ KKA EL+ L G  + +++ ++   VY F+
Sbjct:   34 GRVKIEMKFISNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVMLLVASETGHVYTFA 90

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000029602.1 (pep primary_assembly:ASM223467v1:4:2313004:2320793:-1 gene:ENSORLG00000029337.1 transcript:ENSORLT00000029602.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 9.561e-10
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI ++ I D R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQISRILDQRNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
Match: mef2d (myocyte-specific enhancer factor 2D homolog [Source:NCBI gene;Acc:101174373])

HSP 1 Score: 57.7658 bits (138), Expect = 1.083e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
Match: mef2d (myocyte-specific enhancer factor 2D homolog [Source:NCBI gene;Acc:101174373])

HSP 1 Score: 57.7658 bits (138), Expect = 1.256e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
Match: mef2d (myocyte-specific enhancer factor 2D homolog [Source:NCBI gene;Acc:101174373])

HSP 1 Score: 57.7658 bits (138), Expect = 1.275e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
Match: mef2d (myocyte-specific enhancer factor 2D homolog [Source:NCBI gene;Acc:101174373])

HSP 1 Score: 57.7658 bits (138), Expect = 1.306e-8
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Planmine SMEST
Match: SMESG000011575.1 (SMESG000011575.1)

HSP 1 Score: 517.309 bits (1331), Expect = 0.000e+0
Identity = 312/314 (99.36%), Postives = 312/314 (99.36%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSFYXXXXXXXXXXXXDFNNDTLLDFINKKAQKNAYINDSNSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKENVDILQIKNLNESDQMNAPGGLMAYXXXXXXXXXXXXXXXXXXXXEMPMEFPSKGHEEMITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYKSHXXXXXXXXXXXXXXXXXXXXXETPTFETCSNGIILHLPNILHEEDDRPNLKRIKRFNDSIDT 1048
            MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSFYKKINNKPEKIEEDFNNDTLLDFINKKAQKNAYINDSNSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKENVDILQIKNLNESDQMNAPGGLMAYNFASNNQNIAQI APNNSINEMPMEFPSKGHEE ITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYKSHNYSNTNLNFNNANYQNATNYLETPTFETCSNGIILHLPNILHEEDDRPNLKRIKRFNDSIDT
Sbjct:    1 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSFYKKINNKPEKIEEDFNNDTLLDFINKKAQKNAYINDSNSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKENVDILQIKNLNESDQMNAPGGLMAYNFASNNQNIAQINAPNNSINEMPMEFPSKGHEERITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYKSHNYSNTNLNFNNANYQNATNYLETPTFETCSNGIILHLPNILHEEDDRPNLKRIKRFNDSIDT 314

[Close]

BLAST of MADS-box domain-containing protein vs. Planmine SMEST
Match: SMESG000011575.1 (SMESG000011575.1)

HSP 1 Score: 501.901 bits (1291), Expect = 4.875e-179
Identity = 305/307 (99.35%), Postives = 305/307 (99.35%), Query Frame = 2

Query:  128 MNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSFYXXXXXXXXXXXXDFNNDTLLDFINKKAQKNAYINDSNSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKENVDILQIKNLNESDQMNAPGGLMAYXXXXXXXXXXXXXXXXXXXXEMPMEFPSKGHEEMITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYKSHXXXXXXXXXXXXXXXXXXXXXETPTFETCSNGIILHLPNILHEEDDRPNLKRIKRFNDSIDT 1048
            MNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSFYKKINNKPEKIEEDFNNDTLLDFINKKAQKNAYINDSNSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKENVDILQIKNLNESDQMNAPGGLMAYNFASNNQNIAQI APNNSINEMPMEFPSKGHEE ITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYKSHNYSNTNLNFNNANYQNATNYLETPTFETCSNGIILHLPNILHEEDDRPNLKRIKRFNDSIDT
Sbjct:    1 MNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNKDLVYKFSDPPHSFYKKINNKPEKIEEDFNNDTLLDFINKKAQKNAYINDSNSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKENVDILQIKNLNESDQMNAPGGLMAYNFASNNQNIAQINAPNNSINEMPMEFPSKGHEERITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYKSHNYSNTNLNFNNANYQNATNYLETPTFETCSNGIILHLPNILHEEDDRPNLKRIKRFNDSIDT 307

[Close]

BLAST of MADS-box domain-containing protein vs. Planmine SMEST
Match: SMESG000006802.1 (SMESG000006802.1)

HSP 1 Score: 62.003 bits (149), Expect = 4.592e-10
Identity = 28/49 (57.14%), Postives = 36/49 (73.47%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  IED+R R  TF+KR++GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIQIKRIEDERNRQVTFNKRKMGLMKKAYELSVLCDCEIALIIFN 49

[Close]

BLAST of MADS-box domain-containing protein vs. Planmine SMEST
Match: SMESG000040479.1 (SMESG000040479.1)

HSP 1 Score: 56.225 bits (134), Expect = 3.142e-9
Identity = 27/49 (55.10%), Postives = 34/49 (69.39%), Query Frame = 2

Query:  107 MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVN 253
            MGRKKI +  I+D+R R  TF KR+ GL KKA EL+ LC   I++II N
Sbjct:    1 MGRKKIEIQKIKDERTRQVTFAKRKSGLMKKAYELSVLCDCEIALIIFN 49

The following BLAST results are available for this feature:

BLAST of MADS-box domain-containing protein vs. Ensembl Human
BLAST of MADS-box domain-containing protein vs. Ensembl Celegans
BLAST of MADS-box domain-containing protein vs. Ensembl Fly
BLAST of MADS-box domain-containing protein vs. Ensembl Zebrafish
BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
BLAST of MADS-box domain-containing protein vs. TrEMBL
BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
BLAST of MADS-box domain-containing protein vs. Ensembl Sea Lamprey
BLAST of MADS-box domain-containing protein vs. Ensembl Yeast
BLAST of MADS-box domain-containing protein vs. Ensembl Nematostella
BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
BLAST of MADS-box domain-containing protein vs. Planmine SMEST

BLAST of MADS-box domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
MEF2A	9.608e-10	55.10	myocyte enhancer factor 2A [Source:HGNC Symbol;Acc...	[more]
MEF2C	1.126e-9	55.10	myocyte enhancer factor 2C [Source:HGNC Symbol;Acc...	[more]
MEF2A	1.392e-9	55.10	myocyte enhancer factor 2A [Source:HGNC Symbol;Acc...	[more]
MEF2C	2.136e-9	55.10	myocyte enhancer factor 2C [Source:HGNC Symbol;Acc...	[more]
MEF2D	4.349e-9	55.10	myocyte enhancer factor 2D [Source:HGNC Symbol;Acc...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
mef-2	2.594e-9	53.06	Related to vertebrate MEF2 transcription factor [...	[more]
unc-120	4.789e-6	37.29	pep chromosome:WBcel235:I:8460225:8463015:1 gene:W...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Mef2	2.089e-8	53.19	gene:FBgn0011656 transcript:FBtr0088446	[more]
Mef2	2.262e-8	53.19	gene:FBgn0011656 transcript:FBtr0088444	[more]
Mef2	2.349e-8	53.19	gene:FBgn0011656 transcript:FBtr0088443	[more]
Mef2	2.382e-8	53.19	gene:FBgn0011656 transcript:FBtr0088445	[more]
Mef2	2.442e-8	53.19	gene:FBgn0011656 transcript:FBtr0088447	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mef2d	3.875e-10	55.10	myocyte enhancer factor 2d [Source:ZFIN;Acc:ZDB-GE...	[more]
mef2cb	6.642e-10	55.10	myocyte enhancer factor 2cb [Source:ZFIN;Acc:ZDB-G...	[more]
mef2ca	1.126e-9	55.10	myocyte enhancer factor 2ca [Source:ZFIN;Acc:ZDB-G...	[more]
mef2ca	1.126e-9	55.10	myocyte enhancer factor 2ca [Source:ZFIN;Acc:ZDB-G...	[more]
mef2ca	1.126e-9	55.10	myocyte enhancer factor 2ca [Source:ZFIN;Acc:ZDB-G...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mef2a	4.535e-9	55.10	myocyte enhancer factor 2A [Source:NCBI gene;Acc:1...	[more]
mef2a	1.069e-8	55.10	myocyte enhancer factor 2A [Source:NCBI gene;Acc:1...	[more]
mef2c	1.938e-8	55.10	MADS box transcription enhancer factor 2, polypept...	[more]
mef2d	1.989e-8	55.10	myocyte enhancer factor 2D [Source:NCBI gene;Acc:7...	[more]
mef2c	1.999e-8	55.10	MADS box transcription enhancer factor 2, polypept...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Mef2c	1.041e-9	55.10	myocyte enhancer factor 2C [Source:MGI Symbol;Acc:...	[more]
Mef2c	1.331e-9	55.10	myocyte enhancer factor 2C [Source:MGI Symbol;Acc:...	[more]
Mef2a	2.038e-9	55.10	myocyte enhancer factor 2A [Source:MGI Symbol;Acc:...	[more]
Mef2c	3.156e-9	55.10	myocyte enhancer factor 2C [Source:MGI Symbol;Acc:...	[more]
Mef2c	3.541e-9	55.10	myocyte enhancer factor 2C [Source:MGI Symbol;Acc:...	[more]

BLAST of MADS-box domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q12224\|RLM1_YEAST	1.731e-10	56.90	Transcription factor RLM1 OS=Saccharomyces cerevis...	[more]
sp\|P38128\|SMP1_YEAST	2.275e-9	53.45	Transcription factor SMP1 OS=Saccharomyces cerevis...	[more]
sp\|Q4PSU4\|AGL61_ARATH	2.198e-8	46.88	Agamous-like MADS-box protein AGL61 OS=Arabidopsis...	[more]
sp\|O64703\|AGL29_ARATH	2.431e-8	51.06	Agamous-like MADS-box protein AGL29 OS=Arabidopsis...	[more]
sp\|Q54QY7\|SRFD_DICDI	2.614e-8	55.10	Serum factor response D OS=Dictyostelium discoideu...	[more]

BLAST of MADS-box domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
S8DI54	1.597e-10	51.56	MADS-box domain-containing protein (Fragment) OS=G...	[more]
A0A0G4IL56	2.291e-10	50.88	MADS-box domain-containing protein OS=Plasmodiopho...	[more]
A0A0H5RAW9	1.078e-9	52.63	MADS-box domain-containing protein OS=Spongospora ...	[more]
A0A016U7J8	3.723e-9	49.12	MADS-box domain-containing protein OS=Ancylostoma ...	[more]
A0A4P9ZI70	4.676e-9	56.90	SRF-TF-domain-containing protein (Fragment) OS=Met...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mef2d	1.291e-8	55.10	myocyte enhancer factor 2D [Source:NCBI gene;Acc:1...	[more]
mef2d	1.299e-8	55.10	myocyte enhancer factor 2D [Source:NCBI gene;Acc:1...	[more]
mef2d	1.332e-8	55.10	myocyte enhancer factor 2D [Source:NCBI gene;Acc:1...	[more]
mef2d	1.366e-8	55.10	myocyte enhancer factor 2D [Source:NCBI gene;Acc:1...	[more]
mef2d	1.416e-8	55.10	myocyte enhancer factor 2D [Source:NCBI gene;Acc:1...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of MADS-box domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4


Match Name	E-value	Identity	Description
RLM1	2.492e-12	56.90	MADS-box transcription factor; component of the pr...	[more]
SMP1	3.277e-11	53.45	MADS-box transcription factor involved in osmotic ...	[more]
MCM1	2.592e-9	44.83	Transcription factor; involved in cell-type-specif...	[more]
ARG80	3.763e-8	44.83	Transcription factor involved in regulating argini...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
MEF2	1.332e-9	55.10	MADS-box transcription factor; Myocyte enhancer fa...	[more]
EDO43028	3.162e-7	40.35	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of MADS-box domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000029602.1	9.561e-10	55.10	pep primary_assembly:ASM223467v1:4:2313004:2320793...	[more]
mef2d	1.083e-8	55.10	myocyte-specific enhancer factor 2D homolog [Sourc...	[more]
mef2d	1.256e-8	55.10	myocyte-specific enhancer factor 2D homolog [Sourc...	[more]
mef2d	1.275e-8	55.10	myocyte-specific enhancer factor 2D homolog [Sourc...	[more]
mef2d	1.306e-8	55.10	myocyte-specific enhancer factor 2D homolog [Sourc...	[more]

BLAST of MADS-box domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000011575.1	0.000e+0	99.36	SMESG000011575.1	[more]
SMESG000011575.1	4.875e-179	99.35	SMESG000011575.1	[more]
SMESG000006802.1	4.592e-10	57.14	SMESG000006802.1	[more]
SMESG000040479.1	3.142e-9	55.10	SMESG000040479.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30018122 ID=SMED30018122|Name=MADS-box domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1295bp
TTTAAATACGGTAATAATAAATTAAATATCTATATATTTAAAAAATAGCG
ATAAGTAGTATTATTTTATTCTGAATTTATAAATTTTACATAAAATTATT
TTTGCTATGGGTCGAAAGAAAATCTCTATGAATTTTATAGAAGATGACCG
TAAAAGAGCTAGCACGTTTGACAAACGGAGAATCGGGCTATTTAAAAAGG
CCAGCGAACTGAATCGACTATGTGGAGTCAGTATCTCAGTTATTATTGTT
AACAAAGACCTTGTGTATAAATTCTCGGATCCGCCACATTCATTTTATAA
AAAAATAAATAATAAACCTGAAAAAATTGAAGAAGATTTCAATAATGACA
CATTATTGGATTTTATAAATAAGAAAGCTCAGAAAAATGCGTATATTAAT
GACAGTAATTCAATTGAGCATCATTCATTGGAAGAGGAGCAAAATGGTAC
TTTGACAGACGACGAAGAAGATCAAAGATTCCTGGATGTATTAGATGAAC
ATCATCTGAGAGATGTTTACAATAACATTGATCCAGACATTAGCCCAATA
AAAGAAAATGTCGACATCCTTCAGATAAAGAATCTGAACGAATCAGATCA
AATGAATGCACCAGGAGGTTTGATGGCCTATAATTTTGCTTCCAATAACC
AGAACATTGCTCAAATAATTGCTCCTAACAATTCAATAAACGAAATGCCT
ATGGAATTTCCATCTAAAGGACACGAAGAAATGATAACTTCAACCAAAAT
GGAAAATGTCTACAGTGAGCTAGTTACCGTGTCCACAACGGATACATCAT
ACTTATATGATAATACATCCTACAGTCTCAGTAATTCTAACTGGCAAACC
TATAAGTCGCATAATTATTCAAATACAAACTTAAATTTCAACAATGCAAA
TTATCAGAACGCAACGAATTACTTAGAAACTCCAACGTTTGAAACTTGTA
GCAATGGAATAATACTGCATTTACCCAATATTTTACACGAGGAAGACGAT
CGTCCGAATTTAAAGAGAATTAAACGTTTTAATGATTCGATAGATACCTA
AAGTTTTCATCTTTATTAACATAGCCTAGATTTCCTGGGAATTCAATATT
TTCCGCTTATTTAGAGATTTCAAATCAGGCATATTTTAATATAAGGATTT
TTTATTTTTTGTATATTGCACATCGTGATTAAGTTTTGGTGAAAATTTTA
TTGATTTCTCATGCTTAATTTTTTGAGGGTATTTTAATTACTATTTTCTA
TGAATTTATTGGTTTATAAAATCATTTTAAAATTTTGAGTATTTC

protein sequence of SMED30018122-orf-1

>SMED30018122-orf-1 ID=SMED30018122-orf-1|Name=SMED30018122-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=315bp
MGRKKISMNFIEDDRKRASTFDKRRIGLFKKASELNRLCGVSISVIIVNK
DLVYKFSDPPHSFYKKINNKPEKIEEDFNNDTLLDFINKKAQKNAYINDS
NSIEHHSLEEEQNGTLTDDEEDQRFLDVLDEHHLRDVYNNIDPDISPIKE
NVDILQIKNLNESDQMNAPGGLMAYNFASNNQNIAQIIAPNNSINEMPME
FPSKGHEEMITSTKMENVYSELVTVSTTDTSYLYDNTSYSLSNSNWQTYK
SHNYSNTNLNFNNANYQNATNYLETPTFETCSNGIILHLPNILHEEDDRP
NLKRIKRFNDSIDT*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000026	gut
PLANA:0000034	epidermis
PLANA:0000070	intestinal phagocyte
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036879	TF_MADSbox_sf
IPR033897	MADS_SRF-like
IPR002100	TF_MADSbox

Vocabulary: **molecular function**
Term	Definition
GO:0000981	RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0000987	core promoter proximal region sequence-specific DNA binding
GO:0046983	protein dimerization activity
GO:0003677	DNA binding
GO:0000977	RNA polymerase II regulatory region sequence-specific DNA binding

Vocabulary: **biological process**
Term	Definition
GO:0000165	MAPK cascade
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002100	Transcription factor, MADS-box	PRINTS	PR00404	MADSDOMAIN	coord: 3..23 score: 40.49 coord: 23..38 score: 66.01
IPR002100	Transcription factor, MADS-box	SMART	SM00432	madsneu2	coord: 1..59 e-value: 6.4E-19 score: 78.9
IPR002100	Transcription factor, MADS-box	PFAM	PF00319	SRF-TF	coord: 10..54 e-value: 4.5E-16 score: 58.1
IPR002100	Transcription factor, MADS-box	PROSITE	PS50066	MADS_BOX_2	coord: 1..49 score: 19.133
IPR036879	Transcription factor, MADS-box superfamily	GENE3D	G3DSA:3.40.1810.10		coord: 13..95 e-value: 3.4E-13 score: 51.4
IPR036879	Transcription factor, MADS-box superfamily	SUPERFAMILY	SSF55455	SRF-like	coord: 1..59
None	No IPR available	PANTHER	PTHR11945:SF221	SERUM RESPONSE FACTOR	coord: 4..271
None	No IPR available	PANTHER	PTHR11945	MADS BOX PROTEIN	coord: 4..271
IPR033897	MADS SRF-like	CDD	cd00266	MADS_SRF_like	coord: 2..81 e-value: 9.77029E-21 score: 82.6931

Main menu

MADS-box domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Owlery

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