Ribose-5-phosphate isomerase

Overview
NameRibose-5-phosphate isomerase
Smed IDSMED30018061
Length (bp)860
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ribose-5-phosphate isomerase (SMED30018061) t-SNE clustered cells

Violin plots show distribution of expression levels for Ribose-5-phosphate isomerase (SMED30018061) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ribose-5-phosphate isomerase (SMED30018061) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ribose-5-phosphate isomerase (SMED30018061) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30018061

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30018061h1SMcG0021946 Contig6356newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30018061h1SMcG0021946 Contig6356uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30018061h1SMcG0021946 dd_Smed_v4_12956_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30018061h1SMcG0021946 dd_Smed_v4_12956_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30018061h1SMcG0021946 dd_Smed_v4_12956_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30018061h1SMcG0021946 dd_Smed_v4_12956_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30018061h1SMcG0021946 dd_Smed_v4_12956_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Human
Match: RPIA (ribose 5-phosphate isomerase A [Source:HGNC Symbol;Acc:HGNC:10297])

HSP 1 Score: 267.314 bits (682), Expect = 1.744e-88
Identity = 131/236 (55.51%), Postives = 168/236 (71.19%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            MS  E  KK A   A++ +V+N Q +GIGSGSTI  AV+R+A +V +E L + C PTSFQA+ LIL+Y L L  L+ + E+D+A DGADEVD +L +IKGGGGC  QEKI+A    + I+IAD  KDSK LGD W +GIPIEVIP+AY  +  A+  KFGG   LR A NK GPVVTDNGNFI+DWKF   + + W E++  + MIPG+V+TGLFI+MA  V+FG  DG+V +R K
Sbjct:   75 MSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDWKFD--RVHKWSEVNTAIKMIPGVVDTGLFINMAERVYFGMQDGSVNMREK 308
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Celegans
Match: rpia-1 (Probable-ribose 5-phosphate isomerase [Source:UniProtKB/Swiss-Prot;Acc:P41994])

HSP 1 Score: 209.149 bits (531), Expect = 8.131e-67
Identity = 111/229 (48.47%), Postives = 142/229 (62.01%), Query Frame = 2 
Query:   14 EIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLT-VSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQS-NNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTV 694
            E  KK AA+   +KYVQ+G ++G+GSGST+ + VE L        L  + C PTSF  K  ++E  LP+  L+ + ELD+  DGADEVD   T IKGGGGC  QEKI+    K   +IAD  KDSK LGD +   +PIEV+PLA + +  +I    GG   LRQA  K GP+VTDNGNFI+DW+F+   S  +W  I + L   PG+VETGLFI     VFF  SDG+V
Sbjct:   15 EQAKKRAAFACGEKYVQSGCRLGVGSGSTVKYLVEYLKQGFQNGSLKDIICVPTSFLTKQWLIESGLPVSDLDSHPELDVCIDGADEVDGQFTCIKGGGGCLAQEKIVQTAAKNFYVIADYLKDSKHLGDRYPN-VPIEVLPLAAQPLLRSIPRAEGGSCQLRQAVKKCGPIVTDNGNFIIDWQFEKNVSGRDWFAIQQRLANTPGIVETGLFIGCVDAVFFAYSDGSV 242
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Celegans
Match: rpia-1 (Probable-ribose 5-phosphate isomerase [Source:UniProtKB/Swiss-Prot;Acc:P41994])

HSP 1 Score: 209.149 bits (531), Expect = 8.131e-67
Identity = 111/229 (48.47%), Postives = 142/229 (62.01%), Query Frame = 2 
Query:   14 EIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLT-VSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQS-NNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTV 694
            E  KK AA+   +KYVQ+G ++G+GSGST+ + VE L        L  + C PTSF  K  ++E  LP+  L+ + ELD+  DGADEVD   T IKGGGGC  QEKI+    K   +IAD  KDSK LGD +   +PIEV+PLA + +  +I    GG   LRQA  K GP+VTDNGNFI+DW+F+   S  +W  I + L   PG+VETGLFI     VFF  SDG+V
Sbjct:   15 EQAKKRAAFACGEKYVQSGCRLGVGSGSTVKYLVEYLKQGFQNGSLKDIICVPTSFLTKQWLIESGLPVSDLDSHPELDVCIDGADEVDGQFTCIKGGGGCLAQEKIVQTAAKNFYVIADYLKDSKHLGDRYPN-VPIEVLPLAAQPLLRSIPRAEGGSCQLRQAVKKCGPIVTDNGNFIIDWQFEKNVSGRDWFAIQQRLANTPGIVETGLFIGCVDAVFFAYSDGSV 242
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Fly
Match: Rpi (gene:FBgn0050410 transcript:FBtr0305675)

HSP 1 Score: 243.432 bits (620), Expect = 2.753e-80
Identity = 115/224 (51.34%), Postives = 168/224 (75.00%), Query Frame = 2 
Query:   47 IDKYVQNGQKI-GIGSGSTITFAVERLAHKVIEE-KLT-VSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            +D++V    KI GIGSGST+ +AV+R+A +V +E +LT + C P+S+QA+ LIL+Y L L  L+ N  +D+A DGADEVD+++ +IKGGGGC LQEK++A C K  I++AD +K+S  LG+ W RG+PIEV P+AY  I+  IE  FGGEA LR A  K GP+VTDNGNF++DWKF A +  +W E++R + +IPG++ETGLF++MA + ++G ++G+V ++ K
Sbjct:   18 VDQWVTEDTKILGIGSGSTVVYAVQRIAERVWKEGELTDLICVPSSYQARHLILDYNLNLGDLDRNPNIDVAIDGADEVDRHMVLIKGGGGCLLQEKVVASCAKHFIVVADYTKNSIRLGEQWCRGVPIEVAPMAYVPIKLHIEALFGGEASLRMAKVKAGPIVTDNGNFLLDWKFIANREYDWDEVNRAITLIPGVLETGLFVNMAHKCYYGMANGSVKVQNK 241
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Fly
Match: Rpi (gene:FBgn0050410 transcript:FBtr0301852)

HSP 1 Score: 243.432 bits (620), Expect = 2.753e-80
Identity = 115/224 (51.34%), Postives = 168/224 (75.00%), Query Frame = 2 
Query:   47 IDKYVQNGQKI-GIGSGSTITFAVERLAHKVIEE-KLT-VSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            +D++V    KI GIGSGST+ +AV+R+A +V +E +LT + C P+S+QA+ LIL+Y L L  L+ N  +D+A DGADEVD+++ +IKGGGGC LQEK++A C K  I++AD +K+S  LG+ W RG+PIEV P+AY  I+  IE  FGGEA LR A  K GP+VTDNGNF++DWKF A +  +W E++R + +IPG++ETGLF++MA + ++G ++G+V ++ K
Sbjct:   18 VDQWVTEDTKILGIGSGSTVVYAVQRIAERVWKEGELTDLICVPSSYQARHLILDYNLNLGDLDRNPNIDVAIDGADEVDRHMVLIKGGGGCLLQEKVVASCAKHFIVVADYTKNSIRLGEQWCRGVPIEVAPMAYVPIKLHIEALFGGEASLRMAKVKAGPIVTDNGNFLLDWKFIANREYDWDEVNRAITLIPGVLETGLFVNMAHKCYYGMANGSVKVQNK 241
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Zebrafish
Match: rpia (ribose 5-phosphate isomerase A (ribose 5-phosphate epimerase) [Source:ZFIN;Acc:ZDB-GENE-041114-24])

HSP 1 Score: 292.738 bits (748), Expect = 3.386e-99
Identity = 138/232 (59.48%), Postives = 173/232 (74.57%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D ++QN Q IG+GSGSTI +AV+RLA KV +EKL + C PTSFQA+ LIL++ LPL  L+ + ELD+A DGADEVD  LT+IKGGGGC  QEKI+A C K  I+IAD  KDSK LG  WK+G+P+EVIP+AY  +  AI  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V  R
Sbjct:   41 MAEEAKKLAAYAAVDNHIQNNQVIGVGSGSTIVYAVDRLAEKVRQEKLNIVCVPTSFQARQLILQHGLPLSDLDRHPELDVAIDGADEVDTALTLIKGGGGCLTQEKIVAGCAKHFIVIADYRKDSKALGQQWKKGVPVEVIPMAYVPVSRAIARRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKTR 270
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Zebrafish
Match: rpia (ribose 5-phosphate isomerase A (ribose 5-phosphate epimerase) [Source:ZFIN;Acc:ZDB-GENE-041114-24])

HSP 1 Score: 261.922 bits (668), Expect = 5.210e-87
Identity = 130/235 (55.32%), Postives = 163/235 (69.36%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKV-IEEKLTVSCAPTSF--QAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D ++QN Q IG+GSGSTI +AV+RL     I  K    C       QA+ LIL++ LPL  L+ + ELD+A DGADEVD  LT+IKGGGGC  QEKI+A C K  I+IAD  KDSK LG  WK+G+P+EVIP+AY  +  AI  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V  R
Sbjct:   41 MAEEAKKLAAYAAVDNHIQNNQVIGVGSGSTIVYAVDRLGKTFNISLKRLCLCVTVELLIQARQLILQHGLPLSDLDRHPELDVAIDGADEVDTALTLIKGGGGCLTQEKIVAGCAKHFIVIADYRKDSKALGQQWKKGVPVEVIPMAYVPVSRAIAHRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKTR 273
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Xenopus
Match: ccdc65 (coiled-coil domain containing 65 [Source:Xenbase;Acc:XB-GENE-1002732])

HSP 1 Score: 234.572 bits (597), Expect = 6.247e-77
Identity = 113/208 (54.33%), Postives = 148/208 (71.15%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMV 631
            + E  K+ A + A+D +V+N Q +GIGSGSTI  AV RLA +V +E L V C PTSFQA+ LIL+  L L  L+ + ELD+A DGADEVD  L +IKGGGGC  QEKI+A C K  I+IAD  KDSK  G+ WK+G+PIEVIP+AY  +  A++++FGG A LR A +K GPVVTDNGNF++DWKF   + + W  ++ ++ MIPG V
Sbjct:   13 MTEEAKRLAGWAAVDNHVKNNQALGIGSGSTIVHAVNRLAERVKQENLNVVCVPTSFQARQLILQNGLVLSDLDRHPELDVAIDGADEVDSELNLIKGGGGCLAQEKIVAGCAKSFIVIADYRKDSKNFGEQWKKGVPIEVIPMAYVPVIKALQSRFGGVAELRMAVSKAGPVVTDNGNFLLDWKFD--KVHEWKAVNTEIKMIPGDV 218
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Xenopus
Match: ccdc65 (coiled-coil domain containing 65 [Source:Xenbase;Acc:XB-GENE-1002732])

HSP 1 Score: 231.876 bits (590), Expect = 1.059e-75
Identity = 111/205 (54.15%), Postives = 146/205 (71.22%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIP 622
            + E  K+ A + A+D +V+N Q +GIGSGSTI  AV RLA +V +E L V C PTSFQA+ LIL+  L L  L+ + ELD+A DGADEVD  L +IKGGGGC  QEKI+A C K  I+IAD  KDSK  G+ WK+G+PIEVIP+AY  +  A++++FGG A LR A +K GPVVTDNGNF++DWKF   + + W  ++ ++ MIP
Sbjct:    1 MTEEAKRLAGWAAVDNHVKNNQALGIGSGSTIVHAVNRLAERVKQENLNVVCVPTSFQARQLILQNGLVLSDLDRHPELDVAIDGADEVDSELNLIKGGGGCLAQEKIVAGCAKSFIVIADYRKDSKNFGEQWKKGVPIEVIPMAYVPVIKALQSRFGGVAELRMAVSKAGPVVTDNGNFLLDWKFD--KVHEWKAVNTEIKMIP 203
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Mouse
Match: Rpia (ribose 5-phosphate isomerase A [Source:MGI Symbol;Acc:MGI:103254])

HSP 1 Score: 270.396 bits (690), Expect = 7.029e-90
Identity = 131/236 (55.51%), Postives = 170/236 (72.03%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            MS  E  KK A++ A++ +V+N Q +GIGSGSTI  AV+R+A +V +E L + C PTSFQA+ LIL+Y L L  L+++ E+D+A DGADEVD  L +IKGGGGC  QEKI+A    + I+IAD  KDSK LGD W +GIPIEVIP+AY  +  A+  KFGGE  LR A NK GPVVTDNGNFI+DWKF   + + W E++  + M PG+V+TGLFI+MA  V+FG  DG+V +R K
Sbjct:   68 MSKAEEAKKLASHTAVENHVKNNQVLGIGSGSTIVHAVQRIAERVKQENLDLICIPTSFQARQLILQYGLTLSDLDQHPEIDLAIDGADEVDAELNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDRWHKGIPIEVIPMAYVPVSRAVAQKFGGEVELRMAVNKAGPVVTDNGNFILDWKFD--RVHKWSEVNTAIKMTPGVVDTGLFINMAERVYFGMQDGSVNVREK 301
[Close]
BLAST of Ribose-5-phosphate isomerase vs. UniProt/SwissProt
Match: sp|P47968|RPIA_MOUSE (Ribose-5-phosphate isomerase OS=Mus musculus OX=10090 GN=Rpia PE=1 SV=2)

HSP 1 Score: 270.396 bits (690), Expect = 4.917e-89
Identity = 131/236 (55.51%), Postives = 170/236 (72.03%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            MS  E  KK A++ A++ +V+N Q +GIGSGSTI  AV+R+A +V +E L + C PTSFQA+ LIL+Y L L  L+++ E+D+A DGADEVD  L +IKGGGGC  QEKI+A    + I+IAD  KDSK LGD W +GIPIEVIP+AY  +  A+  KFGGE  LR A NK GPVVTDNGNFI+DWKF   + + W E++  + M PG+V+TGLFI+MA  V+FG  DG+V +R K
Sbjct:   68 MSKAEEAKKLASHTAVENHVKNNQVLGIGSGSTIVHAVQRIAERVKQENLDLICIPTSFQARQLILQYGLTLSDLDQHPEIDLAIDGADEVDAELNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDRWHKGIPIEVIPMAYVPVSRAVAQKFGGEVELRMAVNKAGPVVTDNGNFILDWKFD--RVHKWSEVNTAIKMTPGVVDTGLFINMAERVYFGMQDGSVNVREK 301
[Close]
BLAST of Ribose-5-phosphate isomerase vs. UniProt/SwissProt
Match: sp|P49247|RPIA_HUMAN (Ribose-5-phosphate isomerase OS=Homo sapiens OX=9606 GN=RPIA PE=1 SV=3)

HSP 1 Score: 267.314 bits (682), Expect = 8.377e-88
Identity = 131/236 (55.51%), Postives = 168/236 (71.19%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            MS  E  KK A   A++ +V+N Q +GIGSGSTI  AV+R+A +V +E L + C PTSFQA+ LIL+Y L L  L+ + E+D+A DGADEVD +L +IKGGGGC  QEKI+A    + I+IAD  KDSK LGD W +GIPIEVIP+AY  +  A+  KFGG   LR A NK GPVVTDNGNFI+DWKF   + + W E++  + MIPG+V+TGLFI+MA  V+FG  DG+V +R K
Sbjct:   75 MSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDWKFD--RVHKWSEVNTAIKMIPGVVDTGLFINMAERVYFGMQDGSVNMREK 308
[Close]
BLAST of Ribose-5-phosphate isomerase vs. UniProt/SwissProt
Match: sp|Q3T186|RPIA_BOVIN (Ribose-5-phosphate isomerase (Fragment) OS=Bos taurus OX=9913 GN=RPIA PE=2 SV=2)

HSP 1 Score: 263.462 bits (672), Expect = 6.316e-87
Identity = 130/236 (55.08%), Postives = 167/236 (70.76%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            MS  E  KK A   A++ +V+N Q +GIGSGSTI  AV+R+A +V +E L + C PTSFQA+ LIL+Y L L  L+ + E+D+A DGADEVD +L +IKGGGGC  QEKI+A    + I+IAD  KDSK LGD W +GIPIEVIP+AY  +   +  KFGG   LR A NK GPVVTDNGNFI+DWKF   + + W E++  + MIPG+V+TGLFI+MA  V+FG  DG+V +R K
Sbjct:   29 MSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVEQENLKLVCIPTSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGNASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRTVTQKFGGVIELRMAVNKAGPVVTDNGNFILDWKFD--RVHKWSEVNIAIKMIPGVVDTGLFINMAERVYFGMQDGSVNMREK 262
[Close]
BLAST of Ribose-5-phosphate isomerase vs. UniProt/SwissProt
Match: sp|A2TLM1|RPIA_PIG (Ribose-5-phosphate isomerase OS=Sus scrofa OX=9823 GN=RPIA PE=2 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 3.474e-86
Identity = 129/236 (54.66%), Postives = 167/236 (70.76%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            MS  E  KK A   A++ +V+N Q +GIGSGSTI  AV+R+A +V +E L + C PTSFQA+ LIL++ L L  L+ + E+D+A DGADEVD +L +IKGGGGC  QEKI+A    + I+IAD  KDSK LGD W +GIPIEVIP+AY  +   +  KFGG   LR A NK GPVVTDNGNFI+DWKF   + + W E++  + MIPG+V+TGLFI+MA  V+FG  DG+V +R K
Sbjct:   71 MSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLICIPTSFQARQLILQHGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGNASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRTVTQKFGGVIELRMAVNKAGPVVTDNGNFILDWKFD--RVHKWSEVNTAITMIPGVVDTGLFINMAERVYFGMQDGSVNMREK 304
[Close]
BLAST of Ribose-5-phosphate isomerase vs. UniProt/SwissProt
Match: sp|Q551C2|RPIA_DICDI (Ribose-5-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=rpiA PE=3 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 5.100e-80
Identity = 126/236 (53.39%), Postives = 166/236 (70.34%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIE-TKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRT 706
            MSL    KK AAY A+D +V++G KIGIGSGSTI +AV+R+    ++    V C PTSFQ+  LI+E  L L  L    ELDI  DGADEVDK+  +IKGGGGC LQEKI+AY +KKL+I+AD +K+S ELG+NWK+GIPIEV+P+AY  +   +E + F     LR A NK GPVVTDNGNFI+D +F    S N  ++  D+ MIPG+VETGLF++M    +FG++DGT  +++
Sbjct:    1 MSLDNC-KKVAAYKAVDDFVKDGCKIGIGSGSTIKYAVDRIKELGLKN---VICVPTSFQSTQLIVEAGLELSDLSRTPELDITIDGADEVDKDFNLIKGGGGCQLQEKIVAYSSKKLVIVADHTKESTELGENWKKGIPIEVVPMAYVPVMKKLESSSFSLTPKLRMAVNKAGPVVTDNGNFIIDAQFSKPLS-NIPQLAIDIKMIPGVVETGLFVNMTKIAYFGQTDGTCKVKS 231
[Close]
BLAST of Ribose-5-phosphate isomerase vs. TrEMBL
Match: A0A1A8B7J9 (Ribose 5-phosphate isomerase A (Ribose 5-phosphate epimerase) OS=Nothobranchius furzeri OX=105023 GN=RPIA PE=3 SV=1)

HSP 1 Score: 293.123 bits (749), Expect = 1.562e-96
Identity = 138/232 (59.48%), Postives = 173/232 (74.57%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D YVQN Q IG+GSGSTI +AV+RLA +V +EKL V C PTSFQA+ LIL++ L L  L+ + ELDIA DGADEVD  LT+IKGGGGC  QEKI+A C K+ +++AD  KDSK LG  WK+G+PIEVIP+AY  +   I  +FGGE +LR A +K GPVVTDN NFI+DWKF+ GQ  NW E++  + MIPG+VETGLF++MA   +FG  DG+V IR
Sbjct:    1 MAEEAKKLAAYAAVDNYVQNNQVIGVGSGSTIVYAVDRLAERVRQEKLNVVCVPTSFQARQLILQHGLTLSDLDRHSELDIAIDGADEVDSELTLIKGGGGCLTQEKIVAGCAKRFVVVADYRKDSKALGQQWKKGVPIEVIPMAYVPVSRMIVRRFGGETVLRMAVSKAGPVVTDNSNFILDWKFEHGQ--NWKEVNTAIKMIPGVVETGLFVNMAERAYFGMEDGSVQIR 230
[Close]
BLAST of Ribose-5-phosphate isomerase vs. TrEMBL
Match: A0A1A7WV47 (Ribose 5-phosphate isomerase A (Ribose 5-phosphate epimerase) OS=Iconisemion striatum OX=60296 GN=RPIA PE=3 SV=1)

HSP 1 Score: 293.893 bits (751), Expect = 2.599e-96
Identity = 139/233 (59.66%), Postives = 173/233 (74.25%), Query Frame = 2 
Query:    5 SLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            ++ E  KK AAY A+D YVQN Q IG+GSGSTI +AV+RLA +V +EKL V C PTSFQA+ LIL++ L L  L+ + ELD+A DGADEVD  LT+IKGGGGC  QEKI+A C K  ++IAD  KDSK LG  WK+G+PIEVIP+AY  I   I  +FGGEA+LR A +K GPVVTDN NFI+DWKF+   S NW E++  + MIPG+VETGLF++MA   +FG  DG+V IR
Sbjct:   39 NMAEEAKKLAAYAAVDNYVQNNQVIGVGSGSTIVYAVDRLAERVRQEKLNVVCVPTSFQARQLILQHGLTLSDLDRHSELDVAIDGADEVDSELTLIKGGGGCLTQEKIVAGCAKHFVVIADYRKDSKALGQQWKKGVPIEVIPMAYVPISRTIARRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFE--HSQNWKEVNTAIKMIPGVVETGLFVNMAERAYFGMEDGSVQIR 269
[Close]
BLAST of Ribose-5-phosphate isomerase vs. TrEMBL
Match: Q6NXA0 (Rpia protein (Fragment) OS=Danio rerio OX=7955 GN=rpia PE=2 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 3.237e-96
Identity = 138/232 (59.48%), Postives = 173/232 (74.57%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D ++QN Q IG+GSGSTI +AV+RLA KV +EKL + C PTSFQA+ LIL++ LPL  L+ + ELD+A DGADEVD  LT+IKGGGGC  QEKI+A C K  I+IAD  KDSK LG  WK+G+P+EVIP+AY  +  AI  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V  R
Sbjct:    7 MAEEAKKLAAYAAVDNHIQNNQVIGVGSGSTIVYAVDRLAEKVRQEKLNIVCVPTSFQARQLILQHGLPLSDLDRHPELDVAIDGADEVDTALTLIKGGGGCLTQEKIVAGCAKHFIVIADYRKDSKALGQQWKKGVPVEVIPMAYVPVSRAIAHRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKTR 236
[Close]
BLAST of Ribose-5-phosphate isomerase vs. TrEMBL
Match: B8JI15 (Ribose 5-phosphate isomerase A (ribose 5-phosphate epimerase) OS=Danio rerio OX=7955 GN=rpia PE=1 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 7.577e-96
Identity = 138/232 (59.48%), Postives = 173/232 (74.57%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D ++QN Q IG+GSGSTI +AV+RLA KV +EKL + C PTSFQA+ LIL++ LPL  L+ + ELD+A DGADEVD  LT+IKGGGGC  QEKI+A C K  I+IAD  KDSK LG  WK+G+P+EVIP+AY  +  AI  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V  R
Sbjct:   41 MAEEAKKLAAYAAVDNHIQNNQVIGVGSGSTIVYAVDRLAEKVRQEKLNIVCVPTSFQARQLILQHGLPLSDLDRHPELDVAIDGADEVDTALTLIKGGGGCLTQEKIVAGCAKHFIVIADYRKDSKALGQQWKKGVPVEVIPMAYVPVSRAIARRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKTR 270
[Close]
BLAST of Ribose-5-phosphate isomerase vs. TrEMBL
Match: A0A3N0XXM0 (Ribose-5-phosphate isomerase OS=Anabarilius grahami OX=495550 GN=DPX16_10136 PE=3 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 1.538e-95
Identity = 136/232 (58.62%), Postives = 173/232 (74.57%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D ++QN Q +G+GSGSTI +AV+RLA +V +EKL + C PTSFQA+ LIL++ LPL  L+ + ELD+A DGADEVD  LT+IKGGGGC  QEKI+A C K  I+IAD  KDSK LG  WK+G+P+EVIP+AY  +  AI  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V  R
Sbjct:   39 MAEEAKKLAAYAAVDNHIQNNQVVGVGSGSTIVYAVDRLAERVRQEKLNIVCVPTSFQARQLILQHGLPLSDLDRHPELDVAIDGADEVDAALTLIKGGGGCLTQEKIVAGCAKHFIVIADYRKDSKALGQQWKKGVPVEVIPMAYVPVSRAIARRFGGEAVLRMAVSKAGPVVTDNNNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKTR 268
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Cavefish
Match: rpia (ribose-5-phosphate isomerase [Source:NCBI gene;Acc:103042087])

HSP 1 Score: 287.345 bits (734), Expect = 8.779e-97
Identity = 134/232 (57.76%), Postives = 173/232 (74.57%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D ++QN Q +G+GSGSTI +AV+RLA +V +EKL + C PTSFQA+ LIL++ L L  L+ + ELD+A DGADEVD +LT+IKGGGGC  QEKI+A C K  ++IAD  KDSK LG  WK+G+P+EVIP+AY  +   I  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V IR
Sbjct:   60 MAEEAKKLAAYAAVDNHIQNNQIVGVGSGSTIVYAVDRLAERVRQEKLKIICVPTSFQARQLILKHGLSLSDLDRHPELDVAIDGADEVDASLTLIKGGGGCLTQEKIVAGCAKHFVVIADYRKDSKALGQQWKKGVPVEVIPMAYVPVSRTIARRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKIR 289
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Cavefish
Match: ENSAMXT00000041945.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001650.1:547:7606:1 gene:ENSAMXG00000035270.1 transcript:ENSAMXT00000041945.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 198.364 bits (503), Expect = 9.070e-63
Identity = 109/216 (50.46%), Postives = 137/216 (63.43%), Query Frame = 2 
Query:   59 VQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQ-AKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            V N Q +G+GSGSTI +AV+RL    I     + C     Q A+ LIL++ L L  L+ + ELD+A DGADEVD +LT+IKGGGGC  QEKI+A C K  ++IAD  KDSK LG                   Q  I  +FGGEA+LR A +K GPVVTDN NFI+DWKF+  Q  NW E++  + MIPG+VETGLF+ MA  V+FG  DG+V IR
Sbjct:   39 VINNQIVGVGSGSTIVYAVDRLGKFYI-----LLCVFLCKQCARQLILKHGLSLSDLDRHPELDVAIDGADEVDASLTLIKGGGGCLTQEKIVAGCAKHFVVIADYRKDSKALG-------------------QQTIARRFGGEAVLRMAVSKAGPVVTDNSNFILDWKFEHAQ--NWKEVNTAIKMIPGVVETGLFVGMAERVYFGMEDGSVKIR 228
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Yeast
Match: RKI1 (Ribose-5-phosphate ketol-isomerase; catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis [Source:SGD;Acc:S000005621])

HSP 1 Score: 201.445 bits (511), Expect = 1.964e-64
Identity = 108/242 (44.63%), Postives = 161/242 (66.53%), Query Frame = 2 
Query:   14 EIGKKAAAYFAIDKYVQ--NGQKIGIGSGSTITFAVERLAHKVIEEKL-----TVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDS-KELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAI-LRQAAN-KMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTK 709
            E  K+AAAY A+D+ ++  + + IGIGSGST+ +  ER+   + + K         C PT FQ++ LIL+ KL L S+E+   +DIAFDGADEVD+NL +IKGGG C  QEK+++   K  I++AD  K S K LG NW++G+PIE++P +Y  ++N +  +   E + +RQ  + K GPVVTDN NFI+D  F  G+ ++  ++ R++ ++ G+VETGLFID AS+ +FG SDG+V +  K
Sbjct:   19 EDAKRAAAYRAVDENLKFDDHKIIGIGSGSTVVYVAERIGQYLHDPKFYEVASKFICIPTGFQSRNLILDNKLQLGSIEQYPRIDIAFDGADEVDENLQLIKGGGACLFQEKLVSTSAKTFIVVADSRKKSPKHLGKNWRQGVPIEIVPSSYVRVKNDLLEQLHAEKVDIRQGGSAKAGPVVTDNNNFIIDADF--GEISDPRKLHREIKLLVGVVETGLFIDNASKAYFGNSDGSVEVTEK 258
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Nematostella
Match: EDO31708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX61])

HSP 1 Score: 278.1 bits (710), Expect = 1.804e-94
Identity = 132/230 (57.39%), Postives = 174/230 (75.65%), Query Frame = 2 
Query:   14 EIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            E GKKAAA +A++K+V+NG  +GIGSGST+ +AVERLA +V++EKL V C PTSFQA+ LI E KL L  LE N ELD+A DGADEVD NLT+IKGGGGC  QEKI+A C K L+IIAD+ KDSK+LG+ W +G+P+EV+P AY+ +   +E K G   +LR A  K GPVVTDNGNFI+D  F   Q  +W +++ DL++IPG++ETGLF++M + V FG+ DG++  R
Sbjct:    2 EAGKKAAAVYAVNKFVKNGHVLGIGSGSTVVYAVERLAKRVMKEKLEVVCVPTSFQAQQLITENKLVLSDLERNPELDVAIDGADEVDANLTLIKGGGGCLTQEKIVASCAKTLVIIADDRKDSKQLGEKWSKGVPVEVVPFAYRPVMLKME-KMGLSPVLRMAKAKAGPVVTDNGNFIIDVGFSKPQ--DWNKLNTDLNLIPGVLETGLFVNMTNHVVFGKEDGSIATR 228
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Medaka
Match: rpia (ribose 5-phosphate isomerase A [Source:NCBI gene;Acc:101166762])

HSP 1 Score: 283.493 bits (724), Expect = 1.428e-95
Identity = 130/232 (56.03%), Postives = 175/232 (75.43%), Query Frame = 2 
Query:    8 LKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIR 703
            + E  KK AAY A+D +VQN Q +G+GSGSTI +AV+RLA +V++EKL + C PTSFQA+ LIL++ L L  L+ + ELD+A DGADEVD +LT+IKGGGGC  QEKI+A C K  ++IAD  KDS+ LG  W++GIPIEVIP+A+  I   I  +FGGE  LR A +K GPVVTDN NF++DWKFK  +++NW +++ ++ MIPG+VETGLF++MA   +FG  DG+V +R
Sbjct:   37 MAEEAKKLAAYAAVDNHVQNNQVVGVGSGSTIVYAVDRLAERVMQEKLNIVCVPTSFQARQLILQHGLALSDLDRHPELDVAIDGADEVDADLTLIKGGGGCLTQEKIVAGCAKSFVVIADYRKDSEALGQQWRKGIPIEVIPMAWVPISRVIARRFGGEVNLRMAVSKAGPVVTDNSNFLLDWKFK--EAHNWKKVNTEIKMIPGVVETGLFVNMAERAYFGMEDGSVKVR 266
[Close]
BLAST of Ribose-5-phosphate isomerase vs. Planmine SMEST
Match: SMESG000004984.1 (SMESG000004984.1)

HSP 1 Score: 487.263 bits (1253), Expect = 1.062e-176
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 2 
Query:    2 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTKNN 715
            MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTKNN
Sbjct:    1 MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKLTVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKGGGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKIIQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEIDRDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTKNN 238
The following BLAST results are available for this feature:
BLAST of Ribose-5-phosphate isomerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RPIA1.744e-8855.51ribose 5-phosphate isomerase A [Source:HGNC Symbol... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpia-18.131e-6748.47Probable-ribose 5-phosphate isomerase [Source:Uni... [more]
rpia-18.131e-6748.47Probable-ribose 5-phosphate isomerase [Source:Uni... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rpi2.753e-8051.34gene:FBgn0050410 transcript:FBtr0305675[more]
Rpi2.753e-8051.34gene:FBgn0050410 transcript:FBtr0301852[more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpia3.386e-9959.48ribose 5-phosphate isomerase A (ribose 5-phosphate... [more]
rpia5.210e-8755.32ribose 5-phosphate isomerase A (ribose 5-phosphate... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ccdc656.247e-7754.33coiled-coil domain containing 65 [Source:Xenbase;A... [more]
ccdc651.059e-7554.15coiled-coil domain containing 65 [Source:Xenbase;A... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rpia7.029e-9055.51ribose 5-phosphate isomerase A [Source:MGI Symbol;... [more]
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BLAST of Ribose-5-phosphate isomerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P47968|RPIA_MOUSE4.917e-8955.51Ribose-5-phosphate isomerase OS=Mus musculus OX=10... [more]
sp|P49247|RPIA_HUMAN8.377e-8855.51Ribose-5-phosphate isomerase OS=Homo sapiens OX=96... [more]
sp|Q3T186|RPIA_BOVIN6.316e-8755.08Ribose-5-phosphate isomerase (Fragment) OS=Bos tau... [more]
sp|A2TLM1|RPIA_PIG3.474e-8654.66Ribose-5-phosphate isomerase OS=Sus scrofa OX=9823... [more]
sp|Q551C2|RPIA_DICDI5.100e-8053.39Ribose-5-phosphate isomerase OS=Dictyostelium disc... [more]
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BLAST of Ribose-5-phosphate isomerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1A8B7J91.562e-9659.48Ribose 5-phosphate isomerase A (Ribose 5-phosphate... [more]
A0A1A7WV472.599e-9659.66Ribose 5-phosphate isomerase A (Ribose 5-phosphate... [more]
Q6NXA03.237e-9659.48Rpia protein (Fragment) OS=Danio rerio OX=7955 GN=... [more]
B8JI157.577e-9659.48Ribose 5-phosphate isomerase A (ribose 5-phosphate... [more]
A0A3N0XXM01.538e-9558.62Ribose-5-phosphate isomerase OS=Anabarilius graham... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpia8.779e-9757.76ribose-5-phosphate isomerase [Source:NCBI gene;Acc... [more]
ENSAMXT00000041945.19.070e-6350.46pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RKI11.964e-6444.63Ribose-5-phosphate ketol-isomerase; catalyzes the ... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO317081.804e-9457.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpia1.428e-9556.03ribose 5-phosphate isomerase A [Source:NCBI gene;A... [more]
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BLAST of Ribose-5-phosphate isomerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000004984.11.062e-176100.00SMESG000004984.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30018061 ID=SMED30018061|Name=Ribose-5-phosphate isomerase|organism=Schmidtea mediterranea sexual|type=transcript|length=860bp
AATGTCCTTGAAAGAAATAGGTAAAAAAGCAGCAGCTTATTTTGCTATTG
ATAAATATGTACAAAATGGTCAGAAGATTGGTATTGGTAGTGGTTCAACT
ATTACATTTGCTGTAGAACGATTGGCTCATAAAGTGATTGAAGAAAAACT
AACAGTTTCTTGTGCGCCAACATCATTTCAAGCTAAATTGTTAATTTTGG
AGTATAAACTACCATTAATATCCTTAGAAGAAAATTATGAACTTGATATT
GCATTTGATGGAGCGGATGAAGTTGATAAAAATTTAACAATGATCAAAGG
GGGAGGCGGTTGTCATTTACAAGAGAAAATTATTGCTTACTGCACTAAAA
AATTAATAATAATTGCTGATGAAAGTAAAGATTCGAAAGAATTGGGAGAC
AATTGGAAACGTGGAATCCCAATAGAAGTGATCCCATTAGCATATAAGAT
AATTCAAAATGCAATTGAAACGAAATTCGGCGGAGAAGCTATTTTACGCC
AAGCTGCTAATAAAATGGGACCTGTAGTAACAGATAATGGGAATTTTATT
GTCGATTGGAAATTCAAGGCTGGCCAATCAAATAATTGGGTGGAAATCGA
TAGGGATTTGCACATGATTCCTGGCATGGTTGAAACTGGTCTTTTTATAG
ACATGGCAAGCGAAGTATTTTTTGGTAGGTCTGATGGAACTGTATTTATT
CGAACAAAGAATAATTTTATATAAAGAATTATTATTGCATTTTTTGCAAT
CTTTGTTCTAGCAAAAATTTTCGATGGCCTTATTATCTTTATATTCGTTG
TATGATTTGTTTTATTCGATGAAATATATCAGAAATATATCATTAGTGCA
ATCTCAAAAC
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protein sequence of SMED30018061-orf-1

>SMED30018061-orf-1 ID=SMED30018061-orf-1|Name=SMED30018061-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=241bp
MSLKEIGKKAAAYFAIDKYVQNGQKIGIGSGSTITFAVERLAHKVIEEKL
TVSCAPTSFQAKLLILEYKLPLISLEENYELDIAFDGADEVDKNLTMIKG
GGGCHLQEKIIAYCTKKLIIIADESKDSKELGDNWKRGIPIEVIPLAYKI
IQNAIETKFGGEAILRQAANKMGPVVTDNGNFIVDWKFKAGQSNNWVEID
RDLHMIPGMVETGLFIDMASEVFFGRSDGTVFIRTKNNFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR037171NagB/RpiA_transferase-like
IPR004788Ribose5P_isomerase_type_A
Vocabulary: molecular function
TermDefinition
GO:0004751ribose-5-phosphate isomerase activity
GO:0016853isomerase activity
Vocabulary: biological process
TermDefinition
GO:0009052pentose-phosphate shunt, non-oxidative branch
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004788Ribose 5-phosphate isomerase, type APFAMPF06026Rib_5-P_isom_Acoord: 54..229
e-value: 1.5E-61
score: 207.0
IPR004788Ribose 5-phosphate isomerase, type ATIGRFAMTIGR00021TIGR00021coord: 8..230
e-value: 5.1E-76
score: 252.8
IPR004788Ribose 5-phosphate isomerase, type APANTHERPTHR11934RIBOSE-5-PHOSPHATE ISOMERASEcoord: 5..236
IPR004788Ribose 5-phosphate isomerase, type ACDDcd01398RPI_Acoord: 8..225
e-value: 2.8026E-99
score: 285.905
NoneNo IPR availableGENE3DG3DSA:3.30.70.260coord: 130..213
e-value: 3.2E-85
score: 287.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.1360coord: 8..231
e-value: 3.2E-85
score: 287.2
NoneNo IPR availableSUPERFAMILYSSF75445D-ribose-5-phosphate isomerase (RpiA), lid domaincoord: 135..216
IPR037171NagB/RpiA transferase-likeSUPERFAMILYSSF100950NagB/RpiA/CoA transferase-likecoord: 8..152