Collagen alpha-6(VI) chain
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30018052 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A6 (collagen type VI alpha 6 chain [Source:HGNC Symbol;Acc:HGNC:27023]) HSP 1 Score: 166.392 bits (420), Expect = 1.770e-40 Identity = 225/931 (24.17%), Postives = 415/931 (44.58%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKH-------VGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPF-LR--QDFXXXXXXXXXXXXXXCKTTPA----IKPQTCS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRAR-QVFKIDYRA----DVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMI----------CDAFKQR----QDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--------NFESSIEDMTTSTNGVFIGKALTSARVLFKS--DTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQ-LDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL-------RECKNELLNSTRKEVPKVIVLVTDGVS 4163
D+ +VD S ++ + +F + I + +K R +L ++++ + EF H+ + L H +GG +I KAL A R+++ N + ++V+L + S + V+ +A+ L++ GV I ++G+ KA E LK ++T F LR +D + +K K I + C S D++F++D S + + E +D ++ + ++ +L+I R+ ++ YS+ET L K ++ I+ L A+ + R +VF + V +I VL T + + +D++++A + L+ +GV I +G+ S E +AS + + + + + F +R + C ++ D+ + ID S S +F F++ +V ++ + R+G + L I+ D+ K I I + GNT G A+ N T L K ++ VP LVVLT+G + +S A + + I++ ++G+ ++ ++ +L +IA GE V D + K I N+ Q CK D++F++ SS + E F +M FMK+L + + + ++ +++F+ N KE+++ + ++I+ M G AL+ F R N K +I+I DGE D + E A +L+++G+ ++++G+ G + P+ +V+NF LQR I +L I +EC + LD++F +DSS SI D + DF+ +V+ D+ N + G + + D F LD F K +IS ++ G+T EAL E + LN K VP+V++++TDG S
Sbjct: 27 DVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDK--LHSEF-HL-STFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVE--EASKALRKDGVKIISVGV-QKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHR---DSEDNVTKAAVNLRREGVTIFTLGIEGASDTQL-EKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAAL-NFTLSLLQKAKKQRGNKVPCHLVVLTNGMSK--DSILEPANRLREEHIRVYAIGI-KEANQTQLREIA--GEEKRVYYVHDFDAL--KDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDIN------KEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVK--EPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQR----IEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLN---KGVPQVLIVITDGES 923 HSP 2 Score: 164.081 bits (414), Expect = 1.055e-39 Identity = 230/996 (23.09%), Postives = 439/996 (44.08%), Query Frame = 3
Query: 1002 DLIFVLDAS-SSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVT--DGADNSPTMKPEVEAEKLKKMGIRVIVIGL-GLSVIKDQLNKMSSTETYYPIED-MSKMALF-------------------DTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI--DYRADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNA-----TDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNA---KTISNEA---CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNR--------GNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKN--ATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELC 3785
D++F+LD S + +D F + +L I +VG++ YS+ ++ L NK+E+ + + L G + A+++ +E S+ +G + +LVT D DN A L++ G+ + +G+ G S QL K++S +P E +SK+ F T + + + K + + + DI L++D S Q ++ F + F + +K R V +A+ +E E + D+ K +E+++ +GG T ALN + S K+K + +V+LTNG+S + + ++ A L+E + + IG+ + + +I + DF K + + V+ C T A K DI+F+VD S E + +++ M + I +I ++ +SD EF+L+ ++ DI AI Q+ G +AL+ Q F R ++ K ++L TD +D + E A +L+ +GV I VG+ ++ E + +EN + + + ++ + IC ++EC ++ LDV+ ID S S+ + + I DF+ +V+ D+ K++ R G + + + +D D ++ I ++ + A+G + A +D++FT+ R + VP+VL+V+TDG + + +A + GI +++VG+ + L SS +++ V+TF L+ + ++ N + C+I + DL+F+M S + FK+M +F+ S+ + V R+ +F+ G +I K IE++ IG AL F+ D +R N + ++++ DG+ D E + A L+ GI ++++G+ + ++ + +K L V NF L++ N I +C
Sbjct: 229 DVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKA-----AVNLRREGVTIFTLGIEGAS--DTQLEKIAS----HPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNF-TLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSI--LEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEIC-TEEACKEMKA----DIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITD---GEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSP----REECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLD------DFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKV--------DLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGE------KEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQD--EVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNIC 1176 HSP 3 Score: 145.976 bits (367), Expect = 2.925e-34 Identity = 207/949 (21.81%), Postives = 425/949 (44.78%), Query Frame = 3
Query: 987 NNQQLDLIFVLDAS-SSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKT---VILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTE--TYYPIEDMSKMALFDTTD-IAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT----HIYDDILKKVESLKHVGGFTRIDKALNLASRSFYL---KSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP-FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDY----LFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGD---LIFVMGSSANKTSERFK-----------QMSDFMKSLTKLTVVDKE---NTRVAILKFNRGNYID---SGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVF-TIGLTGKTSESDL 3695
+ ++ D+ ++D S S+ A + + F + V+ I +VG + Y+D ++ + NK ++ K + + + G T AL + ++ K ++++T+G ++P A +L++ IRV IG+ + QL +++ E YY + D AL D + + Q+ C K + K DI +VD S + + F K + + ++G ++ + +V F++ + +EEF DI ++ + H+G T AL+ S+ F N ++ ++L+T+G + + V+ + A L++ GV I ++G+ ++ +L+ +S P F ++F + + + V C K D++FV+D S + ++ ++ M+ ++ ++ + R + Y+D+ F LD+ TK ++ ++ ++ G AL + +F + R V +++++ TD + + A+ L+ G+ ++ VG+ +P S +E + + +C++ K +C + K+D++ +D STS+ P +F+ +F+ +VQ+ D+ +R RIG F+ + P+ + ++ +I I I GNT IG A+ Y + ++ P+VL+VLTDG + + A++ + GI I SVG+G + +++L I + E V F +L+K N + + N IC AG+ + V+ T E+ + + D +++++ L V E T+V++ F N ++ F+ +N +S++D+T + L S F++ + K V++ DG D + ++++ L+K+G+ T+ L G SDL
Sbjct: 431 DTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEP---ANRLREEHIRVYAIGI-KEANQTQLREIAGEEKRVYY-VHDFD--ALKDIRNQVVQEICTEEACKEM---KADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSD--INKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVK--EPAVVLRQEGVIIYSVGVFG-SNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVL---DVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMF-TEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKV---DCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHR--GIDIYSVGIG-DVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRN----------ICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSV-AFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARG-KVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDL 1342 HSP 4 Score: 58.151 bits (139), Expect = 1.446e-7 Identity = 44/185 (23.78%), Postives = 90/185 (48.65%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIV-PEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVKYTK 701
DI +++D S S +F +++ + F I P+ RV + Y D+ EF K+++ L A+ R++GG+++ G ALN TL + + + +++ L++L++G + D S+ + A +++E+ + +Y + I L +K V +F ++ +V IC + K
Sbjct: 436 DIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNT-GAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKD--SILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACK 617 HSP 5 Score: 54.299 bits (129), Expect = 2.708e-6 Identity = 36/142 (25.35%), Postives = 69/142 (48.59%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM--RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
D+ ++D S+SI +F+ + + F + N R+ + DT EF ++F + +++ G++ + G AL + P+M R + ++LL+L+DG + DE V A ++ +G+ IY+V IG
Sbjct: 1000 DLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHI-GAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDE--VAQAAEALRHRGIDIYSVGIG 1138
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213]) HSP 1 Score: 161.384 bits (407), Expect = 6.060e-39 Identity = 266/1145 (23.23%), Postives = 484/1145 (42.27%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS--STETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXX-------XXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLR-SNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---------TKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQ----------KPTICKAAG---DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN--------GVFIGKALTS-ARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYI--------SSELCDVIKGTN---KECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
D++F++D SS+L L ++ F VI+ L+I QV V Y+D + + K E+ V K++ L+G+ F+ N L S + + EG K ++L+T G +P A++LK+ I IG + +L +++ S+ + P E + + + + S K DI ++D S+ V + F + ++G + R LV F++ V E DIL + L+ GG AL+ + + ++ S+ +L++LLT G S + +QAA L +G+ +G +A++ EL+ ++ P+ +L DF +L V + P +P+ S DI+F+ D S + + + +R + + LN+ +RI++ YSD+ E R DE +K +I +K+++ G +L AL+ A++ + V + +VL +++ D A LK GV + F+ +KN+ E + + I + + + + DV+ +D S + F + +FV R+V++LD+ +DR R+ + ++ E YLN S D+ + ++ + G T G A +++ ++ E VP++L+VLT A+ G+ + +V K GG I +G+G E+ I+ +F + TF L + IS Q+ + +P G D++F++ S + E F+ + ++ L V + TRVA+++F+ D E N SS +++ + + +G AL +R +FK S + + +++I G+ D E + A LK+ G+ FTI E +K SP+ V F L + S ++ ++ T + D++F +DSS + PD + DFV RIVR ++I + +VGVV F+ D EF L + + ++ A++R+ G+ + G+AL L +R E VP+ +VLV G S
Sbjct: 239 DIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGY--RAAEGIPKLLVLITGGKSLDEISQP---AQELKRSSIMAFAIG-NKGADQAELEEIAFDSSLVFIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAGQSED--SYLQAANALTRAGILTFCVG-ASQANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPE---SKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGSEGVR-SGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLN----SYMNKQDVVNAVRQLTLLGGPTP---NTGAALEFVLRNILVSSAGSRITEGVPQLLIVLT--ADRSGDDVRNPSVVVKRGGAVPIGIGIGNA-DITEMQTISFIPDFAVAIPTFRQLGTVQ-QVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPE-FQYVRTLIERLVDYLDVGFDTTRVAVIQFS-----DDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDD--EVDDPAVELKQFGVAPFTIARNADQEEL-VKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPAVESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSNDVFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKS 1346 HSP 2 Score: 130.183 bits (326), Expect = 2.437e-29 Identity = 250/1144 (21.85%), Postives = 479/1144 (41.87%), Query Frame = 3
Query: 1002 DLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT---------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG--------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSA----KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---TH-IYDDILKKVESLKHVGG-----FTRIDKALN--LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIG--LGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTA-------LNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXX-----XXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY----NVKTFTDLEKFNA---KTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFI--------GKALTSA-RVLFKSDTRKNATKTVIIIIDGEITDR--NETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYIS-----------SELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALREC-----KNELLNSTRKEVPKVIVLVTDGVS 4163
D+IF++D S++ ++ + L F N+++ L I + +VGV+ +SD L + K ++ V L G + N R+G S+ EG + ++L++ G + E+ + V GLG L I N + + + D+ + L +AQ + P + K DI +VD SS + L + F + I++ E+G + + ++ +A+ V EF TH +++ V +K + G + +D N S + Y ++ +L+VL+T G S++ + Q A +L+ S + IG D+A+ EE+ S+ F+ +F+ ++ T + P+ S+ DIIF++D S + + +R ++ + SL+I R+ ++ +SD TEF L+ TK DI ++QL+ G G LNT N + R V ++++L T ++ L A L G+ VG S+ + E +A S + + + + + ++ K D++ D S +L + F + DF+ +I+ L+++ + TRI + ++ +V + D + ++ + ++ G +G+A+ A Y+F K + V + LV+L G ++D + P A K G+ + + + EL I S F ++ DL K++ N A P D++F++ S S F + +F++ + + V ++ RVA+++ Y D E Y N + +D+ + + + G AL R + S T+ V ++ DR ++ + ++K+ G IG+ G +++++++ PD A+ + F L IS S L V++ + D++F +D S S GP++ Q +ER+V +D+ + T+V V+ F+ D + EF L+ + K + +AV+R+ G ++G AL K L + + VP+ +VL++ G S
Sbjct: 36 DIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGG---SRVEEGVPQVLVLISAGPSSD-----EIRYGVVALKQASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYAD--TVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKSLDEIS--QPAQELKRSSIMAFAIGNKGADQAELEEIAFDSSLV--FIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSG-LNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAG--QSEDSYLQAANALTRAGILTFCVGASQANKAEL-EQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDGP---ASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGA------PAPVSGEKDVVFLLDGSEGVRSG-FPLLKEFVQRVVESLDVGQDRVRVAVVQ-----YSDRTRPEFYLNSYMNKQDVVNAVRQLTLLGGPTPNTGAALEFVLRNILVSSAGSRITEGVPQLLIVLTADRSGDDVRNPSVVVKRGGAVPIGIGI-GNADITEMQTISFIPDFAVAIPTFRQLGTVQQVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPEF-QYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKS 1142 HSP 3 Score: 83.1889 bits (204), Expect = 4.664e-15 Identity = 114/487 (23.41%), Postives = 218/487 (44.76%), Query Frame = 3
Query: 966 STPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF---------ISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-STETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRICSAKLDIALIVDCSSQ-VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--THIYDDILKKVESLK-HVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL 2342
TP+ + N + D+IF+LD S+++ + F N++ SL I +VG++ +SD L + Q K++I + +L+L G+ +N +G S+ RE + ++L+T G ++ A L + GI +G K +L +++ + Y ++D S + TT ++ ++P + +K DI + D S+ V V L+ + I++ + TR ++ ++++ VE F +IL V+ +K G + AL+ A R ++KS S+ + +VLL G S +RV A +L++SGV + I AD EL+ + P L + + L + K+ P S D++F++D S + + ++ + ++SL++ + R++++ YSD T EF L+ K+D+ A++QL
Sbjct: 423 GTPEVHSNKR--DIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGG---SRIREHVPQLLLLLTAGQSEDSYLQ---AANALTRAGILTFCVGAS-QANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQ--PESKRDILFLFDGSANLVGQFPVVRDFLY--KIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGS-EGVRSGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLNSYMNKQDVVNAVRQL 892 HSP 4 Score: 81.6481 bits (200), Expect = 1.393e-14 Identity = 111/500 (22.20%), Postives = 213/500 (42.60%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFISKG----------REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-------STETYYPIEDMSKMALFDTTDIAQDACQ-------IPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD---ILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDK-ADREELKILSTFPN-PFLRQDFXXXX-----------XXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGN 2357
D++F++D S S + ++ L + ++V V+ +SD ++ L + +K E+Q V +L G + NAL E++S+ EG + ++L++ G + P VE LK+ G+ I + +++L K+S S T+ + + + L T + + Q P P + S DI ++D S V+ + + F R + + +G +K R +V F+N+ V E + Y +L + L+ GG KAL +R+ ++KS S+ + +VL+ G S + V A ++ SG I ++G+GD+ DR EL+ ++ P F ++F+E T P +P+ DI+F++D S + + + + + + ++ + ++ ++ Y+ + T EF L + TKR I AI ++ G
Sbjct: 1027 DVVFLIDGSQSAGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNAL-----EYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVDDPAVE---LKQFGVAPFTI--ARNADQEELVKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPA-VESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSND-VFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKSQDDVSRF--AQVIRSSG--IVSLGVGDRNIDRTELQTITNDPRLVFTVREFRELPNIEERIMNSFGPSAATPAPPGVDTPPPSRPE--KKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEDGDSIQVGLVQYNSDPTDEFFLKDFSTKRQIIDAINKVVYKGG 1508 HSP 5 Score: 62.003 bits (149), Expect = 1.032e-8 Identity = 74/330 (22.42%), Postives = 149/330 (45.15%), Query Frame = 3
Query: 3261 AGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF------------KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKT-SESDLKSVASPDKALFV----DNFSNLQ-RYNAYIS--SELCDVIKG---TNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRI-IFDDGATDIGEALRECKNELLNSTR-----KEVPKVIVLVTDGVS 4163
+ D+IF++ S N S F + DF+ +L + + + RV +++F+ + + F D + ++ + + F G L + + S + + +++I G +D E LK+ VF+ GL + S ++L+ +A+ D +F +F +LQ + YI ++ V+K + + D++F +D SS++G + DF+ ++++ ++I + +V V + R EF + K +I+AV+++ D A G AL +N L S+ + +PK++VL+T G S
Sbjct: 34 SADIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFS--DEPRTMFSLDTYSTKAQVLGAVKALG--FAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSSD--EIRYGVVALKQ--ASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKS 355 HSP 6 Score: 58.5362 bits (140), Expect = 1.266e-7 Identity = 45/157 (28.66%), Postives = 75/157 (47.77%), Query Frame = 3
Query: 108 VILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSE-----IDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
V+ L +G RD+ ++D S S EFQ I + + + + + TRV+ + + D K EFL H+S + AV+ R GG +G AL +I RP + + L+++S G + DE V D A ++++ GV +T+A
Sbjct: 1013 VLQPLPSPGVGGKRDVVFLIDGSQS-AGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIF--KRPLGSRIEEGVPQFLVLISSGKSDDE--VDDPAVELKQFGVAPFTIA 1164 HSP 7 Score: 53.9138 bits (128), Expect = 3.454e-6 Identity = 38/118 (32.20%), Postives = 63/118 (53.39%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALR---ECKNELLNSTRKE--VPKVIVLVTDGVST 4166
D++F +D S++ G + LDF+ ++ + I +VGVV F+ + R F LD ++ K ++ AVK + F G +IG AL E +R E VP+V+VL++ G S+
Sbjct: 36 DIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSS 153
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213]) HSP 1 Score: 161.384 bits (407), Expect = 6.060e-39 Identity = 266/1145 (23.23%), Postives = 484/1145 (42.27%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS--STETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXX-------XXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLR-SNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---------TKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQ----------KPTICKAAG---DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN--------GVFIGKALTS-ARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYI--------SSELCDVIKGTN---KECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
D++F++D SS+L L ++ F VI+ L+I QV V Y+D + + K E+ V K++ L+G+ F+ N L S + + EG K ++L+T G +P A++LK+ I IG + +L +++ S+ + P E + + + + S K DI ++D S+ V + F + ++G + R LV F++ V E DIL + L+ GG AL+ + + ++ S+ +L++LLT G S + +QAA L +G+ +G +A++ EL+ ++ P+ +L DF +L V + P +P+ S DI+F+ D S + + + +R + + LN+ +RI++ YSD+ E R DE +K +I +K+++ G +L AL+ A++ + V + +VL +++ D A LK GV + F+ +KN+ E + + I + + + + DV+ +D S + F + +FV R+V++LD+ +DR R+ + ++ E YLN S D+ + ++ + G T G A +++ ++ E VP++L+VLT A+ G+ + +V K GG I +G+G E+ I+ +F + TF L + IS Q+ + +P G D++F++ S + E F+ + ++ L V + TRVA+++F+ D E N SS +++ + + +G AL +R +FK S + + +++I G+ D E + A LK+ G+ FTI E +K SP+ V F L + S ++ ++ T + D++F +DSS + PD + DFV RIVR ++I + +VGVV F+ D EF L + + ++ A++R+ G+ + G+AL L +R E VP+ +VLV G S
Sbjct: 239 DIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGY--RAAEGIPKLLVLITGGKSLDEISQP---AQELKRSSIMAFAIG-NKGADQAELEEIAFDSSLVFIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAGQSED--SYLQAANALTRAGILTFCVG-ASQANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPE---SKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGSEGVR-SGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLN----SYMNKQDVVNAVRQLTLLGGPTP---NTGAALEFVLRNILVSSAGSRITEGVPQLLIVLT--ADRSGDDVRNPSVVVKRGGAVPIGIGIGNA-DITEMQTISFIPDFAVAIPTFRQLGTVQ-QVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPE-FQYVRTLIERLVDYLDVGFDTTRVAVIQFS-----DDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDD--EVDDPAVELKQFGVAPFTIARNADQEEL-VKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPAVESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSNDVFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKS 1346 HSP 2 Score: 130.183 bits (326), Expect = 2.437e-29 Identity = 250/1144 (21.85%), Postives = 479/1144 (41.87%), Query Frame = 3
Query: 1002 DLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT---------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG--------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSA----KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---TH-IYDDILKKVESLKHVGG-----FTRIDKALN--LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIG--LGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTA-------LNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXX-----XXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY----NVKTFTDLEKFNA---KTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFI--------GKALTSA-RVLFKSDTRKNATKTVIIIIDGEITDR--NETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYIS-----------SELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALREC-----KNELLNSTRKEVPKVIVLVTDGVS 4163
D+IF++D S++ ++ + L F N+++ L I + +VGV+ +SD L + K ++ V L G + N R+G S+ EG + ++L++ G + E+ + V GLG L I N + + + D+ + L +AQ + P + K DI +VD SS + L + F + I++ E+G + + ++ +A+ V EF TH +++ V +K + G + +D N S + Y ++ +L+VL+T G S++ + Q A +L+ S + IG D+A+ EE+ S+ F+ +F+ ++ T + P+ S+ DIIF++D S + + +R ++ + SL+I R+ ++ +SD TEF L+ TK DI ++QL+ G G LNT N + R V ++++L T ++ L A L G+ VG S+ + E +A S + + + + + ++ K D++ D S +L + F + DF+ +I+ L+++ + TRI + ++ +V + D + ++ + ++ G +G+A+ A Y+F K + V + LV+L G ++D + P A K G+ + + + EL I S F ++ DL K++ N A P D++F++ S S F + +F++ + + V ++ RVA+++ Y D E Y N + +D+ + + + G AL R + S T+ V ++ DR ++ + ++K+ G IG+ G +++++++ PD A+ + F L IS S L V++ + D++F +D S S GP++ Q +ER+V +D+ + T+V V+ F+ D + EF L+ + K + +AV+R+ G ++G AL K L + + VP+ +VL++ G S
Sbjct: 36 DIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGG---SRVEEGVPQVLVLISAGPSSD-----EIRYGVVALKQASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYAD--TVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKSLDEIS--QPAQELKRSSIMAFAIGNKGADQAELEEIAFDSSLV--FIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSG-LNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAG--QSEDSYLQAANALTRAGILTFCVGASQANKAEL-EQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDGP---ASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGA------PAPVSGEKDVVFLLDGSEGVRSG-FPLLKEFVQRVVESLDVGQDRVRVAVVQ-----YSDRTRPEFYLNSYMNKQDVVNAVRQLTLLGGPTPNTGAALEFVLRNILVSSAGSRITEGVPQLLIVLTADRSGDDVRNPSVVVKRGGAVPIGIGI-GNADITEMQTISFIPDFAVAIPTFRQLGTVQQVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPEF-QYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKS 1142 HSP 3 Score: 83.1889 bits (204), Expect = 4.664e-15 Identity = 114/487 (23.41%), Postives = 218/487 (44.76%), Query Frame = 3
Query: 966 STPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF---------ISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-STETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRICSAKLDIALIVDCSSQ-VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--THIYDDILKKVESLK-HVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL 2342
TP+ + N + D+IF+LD S+++ + F N++ SL I +VG++ +SD L + Q K++I + +L+L G+ +N +G S+ RE + ++L+T G ++ A L + GI +G K +L +++ + Y ++D S + TT ++ ++P + +K DI + D S+ V V L+ + I++ + TR ++ ++++ VE F +IL V+ +K G + AL+ A R ++KS S+ + +VLL G S +RV A +L++SGV + I AD EL+ + P L + + L + K+ P S D++F++D S + + ++ + ++SL++ + R++++ YSD T EF L+ K+D+ A++QL
Sbjct: 423 GTPEVHSNKR--DIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGG---SRIREHVPQLLLLLTAGQSEDSYLQ---AANALTRAGILTFCVGAS-QANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQ--PESKRDILFLFDGSANLVGQFPVVRDFLY--KIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGS-EGVRSGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLNSYMNKQDVVNAVRQL 892 HSP 4 Score: 81.6481 bits (200), Expect = 1.393e-14 Identity = 111/500 (22.20%), Postives = 213/500 (42.60%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFISKG----------REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-------STETYYPIEDMSKMALFDTTDIAQDACQ-------IPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD---ILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDK-ADREELKILSTFPN-PFLRQDFXXXX-----------XXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGN 2357
D++F++D S S + ++ L + ++V V+ +SD ++ L + +K E+Q V +L G + NAL E++S+ EG + ++L++ G + P VE LK+ G+ I + +++L K+S S T+ + + + L T + + Q P P + S DI ++D S V+ + + F R + + +G +K R +V F+N+ V E + Y +L + L+ GG KAL +R+ ++KS S+ + +VL+ G S + V A ++ SG I ++G+GD+ DR EL+ ++ P F ++F+E T P +P+ DI+F++D S + + + + + + ++ + ++ ++ Y+ + T EF L + TKR I AI ++ G
Sbjct: 1027 DVVFLIDGSQSAGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNAL-----EYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVDDPAVE---LKQFGVAPFTI--ARNADQEELVKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPA-VESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSND-VFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKSQDDVSRF--AQVIRSSG--IVSLGVGDRNIDRTELQTITNDPRLVFTVREFRELPNIEERIMNSFGPSAATPAPPGVDTPPPSRPE--KKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEDGDSIQVGLVQYNSDPTDEFFLKDFSTKRQIIDAINKVVYKGG 1508 HSP 5 Score: 62.003 bits (149), Expect = 1.032e-8 Identity = 74/330 (22.42%), Postives = 149/330 (45.15%), Query Frame = 3
Query: 3261 AGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF------------KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKT-SESDLKSVASPDKALFV----DNFSNLQ-RYNAYIS--SELCDVIKG---TNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRI-IFDDGATDIGEALRECKNELLNSTR-----KEVPKVIVLVTDGVS 4163
+ D+IF++ S N S F + DF+ +L + + + RV +++F+ + + F D + ++ + + F G L + + S + + +++I G +D E LK+ VF+ GL + S ++L+ +A+ D +F +F +LQ + YI ++ V+K + + D++F +D SS++G + DF+ ++++ ++I + +V V + R EF + K +I+AV+++ D A G AL +N L S+ + +PK++VL+T G S
Sbjct: 34 SADIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFS--DEPRTMFSLDTYSTKAQVLGAVKALG--FAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSSD--EIRYGVVALKQ--ASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKS 355 HSP 6 Score: 58.5362 bits (140), Expect = 1.266e-7 Identity = 45/157 (28.66%), Postives = 75/157 (47.77%), Query Frame = 3
Query: 108 VILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSE-----IDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
V+ L +G RD+ ++D S S EFQ I + + + + + TRV+ + + D K EFL H+S + AV+ R GG +G AL +I RP + + L+++S G + DE V D A ++++ GV +T+A
Sbjct: 1013 VLQPLPSPGVGGKRDVVFLIDGSQS-AGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIF--KRPLGSRIEEGVPQFLVLISSGKSDDE--VDDPAVELKQFGVAPFTIA 1164 HSP 7 Score: 53.9138 bits (128), Expect = 3.454e-6 Identity = 38/118 (32.20%), Postives = 63/118 (53.39%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALR---ECKNELLNSTRKE--VPKVIVLVTDGVST 4166
D++F +D S++ G + LDF+ ++ + I +VGVV F+ + R F LD ++ K ++ AVK + F G +IG AL E +R E VP+V+VL++ G S+
Sbjct: 36 DIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSS 153
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A3 (collagen type VI alpha 3 chain [Source:HGNC Symbol;Acc:HGNC:2213]) HSP 1 Score: 161.384 bits (407), Expect = 7.514e-39 Identity = 266/1144 (23.25%), Postives = 481/1144 (42.05%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIG-LGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXX-------XXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLR-SNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---------TKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQ----------KPTICKAAG---DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN--------GVFIGKALTS-ARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYI--------SSELCDVIKGTN---KECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
D++F++D SS+L L ++ F VI+ L+I QV V Y+D + + K E+ V K++ L+G+ F+ N L S + + EG K ++L+T G +P A++LK+ I IG G + + S+ + P E + + + + S K DI ++D S+ V + F + ++G + R LV F++ V E DIL + L+ GG AL+ + + ++ S+ +L++LLT G S + +QAA L +G+ +G +A++ EL+ ++ P+ +L DF +L V + P +P+ S DI+F+ D S + + + +R + + LN+ +RI++ YSD+ E R DE +K +I +K+++ G +L AL+ A++ + V + +VL +++ D A LK GV + F+ +KN+ E + + I + + + + DV+ +D S + F + +FV R+V++LD+ +DR R+ + ++ E YLN S D+ + ++ + G T G A +++ ++ E VP++L+VLT A+ G+ + +V K GG I +G+G E+ I+ +F + TF L + IS Q+ + +P G D++F++ S + E F+ + ++ L V + TRVA+++F+ D E N SS +++ + + +G AL +R +FK S + + +++I G+ D E + A LK+ G+ FTI E +K SP+ V F L + S ++ ++ T + D++F +DSS + PD + DFV RIVR ++I + +VGVV F+ D EF L + + ++ A++R+ G+ + G+AL L +R E VP+ +VLV G S
Sbjct: 445 DIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGY--RAAEGIPKLLVLITGGKSLDEISQP---AQELKRSSIMAFAIGNKGADQAELEEIAFDSSLVFIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAGQSED--SYLQAANALTRAGILTFCVG-ASQANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPE---SKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGSEGVR-SGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLN----SYMNKQDVVNAVRQLTLLGGPTP---NTGAALEFVLRNILVSSAGSRITEGVPQLLIVLT--ADRSGDDVRNPSVVVKRGGAVPIGIGIGNA-DITEMQTISFIPDFAVAIPTFRQLGTVQ-QVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPE-FQYVRTLIERLVDYLDVGFDTTRVAVIQFS-----DDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDD--EVDDPAVELKQFGVAPFTIARNADQEEL-VKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPAVESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSNDVFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKS 1552 HSP 2 Score: 129.413 bits (324), Expect = 3.527e-29 Identity = 251/1144 (21.94%), Postives = 480/1144 (41.96%), Query Frame = 3
Query: 1002 DLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT---------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG--------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSA----KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---TH-IYDDILKKVESLKHVGG-----FTRIDKALN--LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIG--LGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTA-------LNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXX-----XXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY----NVKTFTDLEK--FN-AKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFI--------GKALTSA-RVLFKSDTRKNATKTVIIIIDGEITDR--NETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYIS-----------SELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALREC-----KNELLNSTRKEVPKVIVLVTDGVS 4163
D+IF++D S++ ++ + L F N+++ L I + +VGV+ +SD L + K ++ V L G + N R+G S+ EG + ++L++ G + E+ + V GLG L I N + + + D+ + L +AQ + P + K DI +VD SS + L + F + I++ E+G + + ++ +A+ V EF TH +++ V +K + G + +D N S + Y ++ +L+VL+T G S++ + Q A +L+ S + IG D+A+ EE+ S+ F+ +F+ ++ T + P+ S+ DIIF++D S + + +R ++ + SL+I R+ ++ +SD TEF L+ TK DI ++QL+ G G LNT N + R V ++++L T ++ L A L G+ VG S+ + E +A S + + + + + ++ K D++ D S +L + F + DF+ +I+ L+++ + TRI + ++ +V + D + ++ + ++ G +G+A+ A Y+F K + V + LV+L G ++D + P A K G+ + + + EL I S F ++ DL N K++ N A P D++F++ S S F + +F++ + + V ++ RVA+++ Y D E Y N + +D+ + + + G AL R + S T+ V ++ DR ++ + ++K+ G IG+ G +++++++ PD A+ + F L IS S L V++ + D++F +D S S GP++ Q +ER+V +D+ + T+V V+ F+ D + EF L+ + K + +AV+R+ G ++G AL K L + + VP+ +VL++ G S
Sbjct: 242 DIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGG---SRVEEGVPQVLVLISAGPSSD-----EIRYGVVALKQASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYAD--TVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKSLDEIS--QPAQELKRSSIMAFAIGNKGADQAELEEIAFDSSLV--FIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSG-LNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAG--QSEDSYLQAANALTRAGILTFCVGASQANKAEL-EQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDGP---ASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGA------PAPVSGEKDVVFLLDGSEGVRSG-FPLLKEFVQRVVESLDVGQDRVRVAVVQ-----YSDRTRPEFYLNSYMNKQDVVNAVRQLTLLGGPTPNTGAALEFVLRNILVSSAGSRITEGVPQLLIVLTADRSGDDVRNPSVVVKRGGAVPIGIGI-GNADITEMQTISFIPDFAVAIPTFRQLGTVQQVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPEF-QYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKS 1348 HSP 3 Score: 115.546 bits (288), Expect = 6.169e-25 Identity = 175/752 (23.27%), Postives = 316/752 (42.02%), Query Frame = 3
Query: 2088 TTPAIKPQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRAD--VAKIVVLFTDKLPTNQKDSLS--EAEILKGDGVKIIFVGLS----------KNSPYNFYE-NLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEK-ELNDIASSGEFY----NVKTFTDLEKFNAKTISNEACQ--IPQKPTICKAA-----GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTST--------NGVFIGKALTSARV-LFKSDTRKNAT----KTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDV--IKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELLNST-----RKEVPKVIVLVTDGVS 4163
TT A + Q NG DIIF+VD S E + +R + ++SL + +++ ++ TEF L+ TK+++ I + G L Q RA V +++V+ TD + KD L+ AE+ D V + +G+ + P N + NL + S I + D + +D D+I ID S + G NF + LDF+ +++ L + + R+G+ F+ E +D+ A + + + F G A IG A+ + FT+ E VP+VLV+++ G + S +I L + S G+G Q + + EL IA+ ++F DL++ I A + + + PTI D++F++ S+ F + DF+ + + + ++ +VA+ + Y D+ E Y N + ++ T+ + ++ G AL R LF S A K +++I G+ D E + A LK+ I F IG G +++L+ +A +F+ + L + + GT + S K+ D++F LD S+++G + DFV +V +DI + +VG+V F+ EF L+ + K ++ ++++ G+ + G AL R+ VP++++L+T G S
Sbjct: 22 TTHAQQQQADVKNGAAADIIFLVDSSWTIGEEHFQLVREFLYDVVKSLAVGENDFHFALVQFNGNPHTEFLLNTYRTKQEVLSHISNMSYIGGTNQTGKGLEYIMQSHLTKAAGSRAGDGVPQVIVVLTDG---HSKDGLALPSAELKSAD-VNVFAIGVEDADEGALKEIASEPLNMHMFNLENFTSLHDIVGNLVSCVHSSVSPERAGDT-ETLKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPS----SDEIRYGVVALKQASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQ-----YADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKSLD--EISQPAQELKRSSIMAFAIGNKG-ADQAELEEIAFDSSLVFIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKR-DIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAGQS 755 HSP 4 Score: 82.8037 bits (203), Expect = 5.118e-15 Identity = 114/487 (23.41%), Postives = 218/487 (44.76%), Query Frame = 3
Query: 966 STPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF---------ISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-STETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRICSAKLDIALIVDCSSQ-VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--THIYDDILKKVESLK-HVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL 2342
TP+ + N + D+IF+LD S+++ + F N++ SL I +VG++ +SD L + Q K++I + +L+L G+ +N +G S+ RE + ++L+T G ++ A L + GI +G K +L +++ + Y ++D S + TT ++ ++P + +K DI + D S+ V V L+ + I++ + TR ++ ++++ VE F +IL V+ +K G + AL+ A R ++KS S+ + +VLL G S +RV A +L++SGV + I AD EL+ + P L + + L + K+ P S D++F++D S + + ++ + ++SL++ + R++++ YSD T EF L+ K+D+ A++QL
Sbjct: 629 GTPEVHSNKR--DIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGG---SRIREHVPQLLLLLTAGQSEDSYLQ---AANALTRAGILTFCVGAS-QANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQ--PESKRDILFLFDGSANLVGQFPVVRDFLY--KIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGS-EGVRSGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLNSYMNKQDVVNAVRQL 1098 HSP 5 Score: 81.2629 bits (199), Expect = 1.635e-14 Identity = 111/500 (22.20%), Postives = 213/500 (42.60%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFISKG----------REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-------STETYYPIEDMSKMALFDTTDIAQDACQ-------IPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD---ILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDK-ADREELKILSTFPN-PFLRQDFXXXX-----------XXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGN 2357
D++F++D S S + ++ L + ++V V+ +SD ++ L + +K E+Q V +L G + NAL E++S+ EG + ++L++ G + P VE LK+ G+ I + +++L K+S S T+ + + + L T + + Q P P + S DI ++D S V+ + + F R + + +G +K R +V F+N+ V E + Y +L + L+ GG KAL +R+ ++KS S+ + +VL+ G S + V A ++ SG I ++G+GD+ DR EL+ ++ P F ++F+E T P +P+ DI+F++D S + + + + + + ++ + ++ ++ Y+ + T EF L + TKR I AI ++ G
Sbjct: 1233 DVVFLIDGSQSAGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNAL-----EYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVDDPAVE---LKQFGVAPFTI--ARNADQEELVKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPA-VESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSND-VFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKSQDDVSRF--AQVIRSSG--IVSLGVGDRNIDRTELQTITNDPRLVFTVREFRELPNIEERIMNSFGPSAATPAPPGVDTPPPSRPE--KKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEDGDSIQVGLVQYNSDPTDEFFLKDFSTKRQIIDAINKVVYKGG 1714 HSP 6 Score: 76.2554 bits (186), Expect = 5.945e-13 Identity = 84/331 (25.38%), Postives = 144/331 (43.50%), Query Frame = 3
Query: 3258 AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE---SSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNA--------TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKE-----------CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALR---ECKNELLNSTRKE--VPKVIVLVTDGVST 4166
AA D+IF++ SS E F+ + +F+ + K V + + A+++FN GN Y+ + S I +M+ GK L + +S K A + ++++ DG D A L D + VF IG+ E LK +AS + + N N + + + + V + E + D++F +D S++ G + LDF+ ++ + I +VGVV F+ + R F LD ++ K ++ AVK + F G +IG AL E +R E VP+V+VL++ G S+
Sbjct: 36 AAADIIFLVDSSWTIGEEHFQLVREFLYDVVKSLAVGENDFHFALVQFN-GNPHTEFLLNTYRTKQEVLSHISNMSYIGGTNQTGKGL---EYIMQSHLTKAAGSRAGDGVPQVIVVLTDGHSKD-GLALPSAELKSAD-VNVFAIGVE-DADEGALKEIASEPLNMHMFNLENFTSLHDIVGNLVSCVHSSVSPERAGDTETLKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSS 359 HSP 7 Score: 58.5362 bits (140), Expect = 1.339e-7 Identity = 45/157 (28.66%), Postives = 75/157 (47.77%), Query Frame = 3
Query: 108 VILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSE-----IDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
V+ L +G RD+ ++D S S EFQ I + + + + + TRV+ + + D K EFL H+S + AV+ R GG +G AL +I RP + + L+++S G + DE V D A ++++ GV +T+A
Sbjct: 1219 VLQPLPSPGVGGKRDVVFLIDGSQS-AGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIF--KRPLGSRIEEGVPQFLVLISSGKSDDE--VDDPAVELKQFGVAPFTIA 1370 HSP 8 Score: 57.3806 bits (137), Expect = 3.158e-7 Identity = 38/130 (29.23%), Postives = 65/130 (50.00%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVSTGGL---SPELEST 4196
D++F +DSS +IG +++Q +F+ +V+ + + N +V FN + EF L+ + K ++S + + + G G+ L L VP+VIV++TDG S GL S EL+S
Sbjct: 39 DIIFLVDSSWTIGEEHFQLVREFLYDVVKSLAVGENDFHFALVQFNGNPHTEFLLNTYRTKQEVLSHISNMSYIGGTNQTGKGLEYIMQSHLTKAAGSRAGDGVPQVIVVLTDGHSKDGLALPSAELKSA 168
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Match: COL6A5 (collagen type VI alpha 5 chain [Source:HGNC Symbol;Acc:HGNC:26674]) HSP 1 Score: 148.673 bits (374), Expect = 4.811e-35 Identity = 248/1130 (21.95%), Postives = 491/1130 (43.45%), Query Frame = 3
Query: 1002 DLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSK-LELLEGATFISNALRRSGEEFIS---KGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF--DTTDIAQDACQIPKPKRICSAKLDIALIVDCS--SQVQLHEWVNQLL-----------FAKRFIEQFELGINKTRFSLVSFANEA---------VVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQ-------------TC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKI---VVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL-FTKG-REDVPKVLVVLTDGA--NDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS-SGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG--DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSG----FKEDYKNFESSIEDMTT--STNG-VFIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKE--VPKVIVLVTDG 4157
D++F++D+S L + F N +I SL I+ +V + YSD L + + ++ + + K + + G+ I AL+ + + S GR+ + ILV + S + EVE ++ L+K G+++I +G+ ++ L M+++ ++ + + ++ F + T I +D + + A + + + C S L V++ L F + ++ N R L+S++N A + EF +++++L G + A++ R + +S S+R + VL+T+ S + V AA +L+ V++ + + A+ +L+ + ++P K L S K K I Q C + DI F++D S+ + ++ I+ ML + +I R+ ++ YSD+T EF + + D+ AI ++ G AL+ Q+ K + ++K+ +++ TD + T++ + A+ L+ + + + VG+ + E ++ +N + + K ++ IC + C K D++ +D S S+G ENF+ F+ ++ + + D+T+IG+ F+ + ++ F ++ I+++ I T G A+ Y +KG R K L+++TDG +D+ + +I K + I SVGV + +L +I+ S ++V+ F L+ K I C + CK D++FV+ S + KQ D M +LT + +V K + ++F Y D + Y N + IE++ T G + KAL A LF S ++N + +I+I DGE D ++ + A L+ GI +F +G+ GK ++ +L+ +A + + ++VDNF L+ + +C + C ++ D++F D S + + T F+ ++ + DI + K+G+ F ++ + L K + ++ + G I AL++ N L R+ VP+ +V++T G
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAES---EDEVEEASKALQKDGVKIISVGV-QKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKE--GAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQ-------QQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVH--DAALNLRLEDVNVFALSI-QGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDR--VVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC-----AEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKD----VTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLKALERKLIF-RVCALHD----CKRITLLDVVFVLDHSGSIK----KQYQDHMINLT-IHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGV-GKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTE---APEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSG 1117 HSP 2 Score: 137.887 bits (346), Expect = 9.609e-32 Identity = 167/729 (22.91%), Postives = 336/729 (46.09%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSN----GNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQD------------ECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNA-----TDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS---SGEFYNVKTFTDLEKFNAK-----------TISNEACQIPQKPTIC--KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKT---VIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKAL-FVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVS 4163
D++F+VD S + + +++ + + SL I R+++ YSDE +EF L F R + L+ N G + AL A + + I+ R D + + +D + EA + L+ DGVKII VG+ K S N + +A+ + I + + + +I D K ++ C L D++ +D+S G N + F+ I ++D++++ R+G+ ++++ + S+ ++ Q++I + G + G AI ++ ++ + VP++ V++T +D + +A+ +L + + ++ + Q + +L +I S +K++ DLE ++ K IS A Q T C D+ F++ S++ ++F+Q+ FM +T++ + + RV +++++ ++ + DY N +I ++ T G + GKAL + K+ + +K +I++ DG TDR E A L+ + I V +G+ G ++ +L+ +A ++ + F NF L+ + E+C K C + D+MF +DSS SIG + ++ F++ ++ + I A+ T++GVV F+ ++EF L+++ + + A+ R+ + T G+AL +S R K ++L+TDGV+
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHL-STFKGRS--PMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGR-DRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENL-KAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTG-GNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSD--DEVHDAALNLRLEDVNVFALSI-QGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEF-YITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDR--VVEPAKRLRAEQITVHAVGI-GAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC-----AEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVA 740 HSP 3 Score: 107.457 bits (267), Expect = 1.583e-22 Identity = 176/794 (22.17%), Postives = 354/794 (44.58%), Query Frame = 3
Query: 948 SNYIEISTPDQ-NC-NNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTV----ILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT-----TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY--DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK----NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY----RADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDY--LFTKGRED--VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFN----AKTISNEACQ 3230
S Y E D+ C + ++ D+ F++D SSS+ ++Q F V + I +VGV+ YSD E+ + N +++K + ++ L G T+ AL + I G + V I++TDG ++P A++L+ I V +G+G + NK+ E E +S FD ++ ++ C ++ C K DI +VD S + + +F K + + ++G +KT+ +V F+++ E + + +I ++ + + T KALN + ++ SK +K+ ++L+T+G++ + V+ A L+ V I ++G+ + A+R +L+ +S + F ++F K+L K + C + D++FV+D S + D + +L + ++ ++ R + + YSD+ F L+ + I +++ R G AL A +F ++ + +V +++++ TD ++ D L++ + L+ G+ I VG+ K + +K ++I ++N +K+ ++ +C + + C +++ DVI D S + +F F++ ++ N D++ R +IG+ F S + + ++ + +I + G I +A+ ++ L GR + VP+ LVV+T G + + +K GI ++ +G+G E L +G + TF D +K K I E CQ
Sbjct: 422 STYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYI-LQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEP---AKRLRAEQITVHAVGIGAA------NKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC----AEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQ-YFTHSKGARLGAKKFLILITDGVAQDDVR--DPARILRGKDVTIFSVGVYN-ANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLL----DVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDG-ESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMC---TEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDL--GICVLVLGIGDVYKEHLL---PITGNSEKIITFQDFDKLKNVDVKKRIIREICQ 1184 HSP 4 Score: 105.916 bits (263), Expect = 4.503e-22 Identity = 124/534 (23.22%), Postives = 245/534 (45.88%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINK-IPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF--NAKTISNEACQIPQK----------PTICK--AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED--YKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT----RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS--PDKAL-FVDNFSNLQRYNAY--------ISSELCDVIKGTNKE---C-STKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEA----LRECKNELLNSTRKEVPKVIVLVTDGVST 4166
DV+ +D S LGP++F F+N+++ +L + ++ R+ + ++ E + + + + M + K FI G+ IG A+ A F+ + R+ P +LVVL ++ E + S K G+KIISVGV Q+ SE+ L +A+S +N++T DL F N I + + + P C+ + DL+F++ S T + + F++++T V + R+ ++ ++ S K F+ I++++ G A+ R S++ R + +++ +D +E + A L+ + + VF + + G + + L+ + S P++ + + ++++L+ Y+ I S++ + N + C TK+ D+ F +D SSSI ++ F+ + I + +VGVV ++ D EF + ++ + A+ I G T G+A L+ KN + + K VP ++++TDG+ST
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESE-DEVEEASKALQK-DGVKIISVGV-QKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLG-TGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSD-DEVHDAALNLRLEDVNVFALSIQG-ANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSK-VPCYLIVLTDGMST 556 HSP 5 Score: 56.225 bits (134), Expect = 6.966e-7 Identity = 49/185 (26.49%), Postives = 89/185 (48.11%), Query Frame = 3
Query: 141 KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRP-TSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVK 692
K DI ++D SSSI +F+ + + F I P+ RV + Y D + EF T +++ L A+ +++ G + G+AL+ L I M+ S++ L++L+DG +TD V + A +++ + +T++ V IG+ I L ++ V F ++ +V IC K
Sbjct: 439 KEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTG-GTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDR--VVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEK 620
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: cut-6 (CUTiclin [Source:UniProtKB/TrEMBL;Acc:Q21540]) HSP 1 Score: 73.9442 bits (180), Expect = 4.600e-13 Identity = 42/136 (30.88%), Postives = 73/136 (53.68%), Query Frame = 3
Query: 3759 NAYISSELCDVIKGTNKECST-KQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
N + SEL EC T K ++++ LD+S SIG D ++ +L F + ++I+ + + + ++ + + EF L QF + A++RI + GAT+ G+ALR + L R +PKV +++TDG S
Sbjct: 29 NGLVDSELI-------HECVTHKAVEVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQS 157 HSP 2 Score: 61.6178 bits (148), Expect = 3.379e-9 Identity = 42/194 (21.65%), Postives = 92/194 (47.42%), Query Frame = 3
Query: 2676 DEC-TMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG-EFYNVKTFTDLEKFNAKTISNEACQIPQKP 3245
EC T K ++VI+ +D S S+G + F+ +L F + L++ +D + + + + L + ++ I +I + G T G A+ + R +PKV +V+TDG +D+ E Q+ + + + ++GV ++ +L+ + + + V++F L++ A +++ C+ +P
Sbjct: 38 HECVTHKAVEVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQSQDDVSEPSQL----LRDADVMVYAIGVTNLVNVHQLHQMTGNPVRVFTVESFEQLDRALADSLTWSMCKTEFRP 227 HSP 3 Score: 59.3066 bits (142), Expect = 1.812e-8 Identity = 39/155 (25.16%), Postives = 80/155 (51.61%), Query Frame = 3
Query: 990 NQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSS 1451
++ +++I +LDAS S+ + ++L+F ++ L I S + ++ Y++ ++ LG + T+++ + ++E GAT ALR + E+ + R G K I++TDG +P ++ L+ + V IG+ V QL++M+
Sbjct: 43 HKAVEVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQSQDDVSEP---SQLLRDADVMVYAIGVTNLVNVHQLHQMTG 194
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: cut-6 (CUTiclin [Source:UniProtKB/TrEMBL;Acc:Q21540]) HSP 1 Score: 73.9442 bits (180), Expect = 4.600e-13 Identity = 42/136 (30.88%), Postives = 73/136 (53.68%), Query Frame = 3
Query: 3759 NAYISSELCDVIKGTNKECST-KQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
N + SEL EC T K ++++ LD+S SIG D ++ +L F + ++I+ + + + ++ + + EF L QF + A++RI + GAT+ G+ALR + L R +PKV +++TDG S
Sbjct: 29 NGLVDSELI-------HECVTHKAVEVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQS 157 HSP 2 Score: 61.6178 bits (148), Expect = 3.379e-9 Identity = 42/194 (21.65%), Postives = 92/194 (47.42%), Query Frame = 3
Query: 2676 DEC-TMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG-EFYNVKTFTDLEKFNAKTISNEACQIPQKP 3245
EC T K ++VI+ +D S S+G + F+ +L F + L++ +D + + + + L + ++ I +I + G T G A+ + R +PKV +V+TDG +D+ E Q+ + + + ++GV ++ +L+ + + + V++F L++ A +++ C+ +P
Sbjct: 38 HECVTHKAVEVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQSQDDVSEPSQL----LRDADVMVYAIGVTNLVNVHQLHQMTGNPVRVFTVESFEQLDRALADSLTWSMCKTEFRP 227 HSP 3 Score: 59.3066 bits (142), Expect = 1.812e-8 Identity = 39/155 (25.16%), Postives = 80/155 (51.61%), Query Frame = 3
Query: 990 NQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSS 1451
++ +++I +LDAS S+ + ++L+F ++ L I S + ++ Y++ ++ LG + T+++ + ++E GAT ALR + E+ + R G K I++TDG +P ++ L+ + V IG+ V QL++M+
Sbjct: 43 HKAVEVILLLDASGSIGDDTFKKQLSFAMHLASRLNISEDGSHMALIQYAETPKLEFSLGQFNHPTQLEWAIQRIEYQSGATNTGQALRLTLEKGLQGARPGIPKVAIVITDGQSQDDVSEP---SQLLRDADVMVYAIGVTNLVNVHQLHQMTG 194
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: mup-4 (Transmembrane matrix receptor MUP-4 [Source:UniProtKB/Swiss-Prot;Acc:Q21281]) HSP 1 Score: 71.633 bits (174), Expect = 5.203e-12 Identity = 41/123 (33.33%), Postives = 66/123 (53.66%), Query Frame = 3
Query: 3813 CSTKQLDLMFALDSSSSIGPDYWQTE-LDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTR-----KEVPKVIVLVTDGVS 4163
C + DL+F +D S SIG +QTE L F+ DI T+V VV ++ R EFGLD ++++ ++ +A++ I + G T G A+ NE + R +V +V +++TDG S
Sbjct: 431 CPAQPTDLVFLIDGSGSIGSYVFQTEVLRFLAEFTELFDIAPQKTRVSVVQYSDQIRHEFGLDNYSDRKSLQNAIRNIEYLTGLTRTGAAIEHVANEAFSERRGARPVGQVSRVAIVITDGRS 553 HSP 2 Score: 60.8474 bits (146), Expect = 8.923e-9 Identity = 35/136 (25.74%), Postives = 67/136 (49.26%), Query Frame = 3
Query: 111 ILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIA-INQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPE---MRPTSEIDRILLILSDGSNTDELS 506
+ L C +P D+ ++D S SI + FQ+ ++ + +F F I P TRVS + Y D ++ EF ++ +L A+R + G + G A+ + E RP ++ R+ ++++DG + D ++
Sbjct: 424 VCTLSTACPAQPTDLVFLIDGSGSIGSYVFQTEVLRFLAEFTELFDIAPQKTRVSVVQYSDQIRHEFGLDNYSDRKSLQNAIRNIEYLTGLTRT-GAAIEHVANEAFSERRGARPVGQVSRVAIVITDGRSQDNVT 558
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: cutl-23 (CUTiclin-Like [Source:UniProtKB/TrEMBL;Acc:A0A168H2V5]) HSP 1 Score: 67.0106 bits (162), Expect = 8.543e-11 Identity = 71/321 (22.12%), Postives = 153/321 (47.66%), Query Frame = 3
Query: 3267 DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRK----NATKTVIIIIDGEITDRNETFEEANLLKKD--GIKVFTIGLTGKTSESDLKSVASPDKALFVD-NFSNLQ-RYNAYISSELCD----VIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFT--EKYAMISAVKRIIFDDGATDIGEALRECKNELLNS------TRKEVPKVIVLVTDGVS 4163
DL+F++ SS + ++F+ D ++ + K + K TRV +++F+ +++ F++ D + ++++ + + IG A A K+ K V ++ DG D + ++ A++L+++ I ++ G + + L + ++ + + N L+ ++ Y +E+C+ +KG++K LDL +D+S S+ + + VER++ +++I+ +V ++T++ A F + F ++ VK I G T AL + +LL S R+ VPK+ +++TDG S
Sbjct: 54 DLVFILDSSGS-LRDKFQDEIDIIRRILKHVTIGKTATRVMLIQFSGTQHLEFNFEKFTDREELLAALDVLRHVSGITRIGGAFEFALQQLKTPGSGLRDGTVPKIVYLLSDGRTHDFPKDWQMADILRREIPNIDIWAYGTGDYVAMAALANYTQSEQKIVTNLNLQKLEPQFEKYHGTEICEQQPSCVKGSDKP-----LDLALVVDASESLDHLFSDQKKFLVERVLGNINIHPEAVRVALITYSGQAFVHFKFNSFLYGNNTSVQGFVKNIRSIKGTTATNVALMD-AFDLLTSKDPSIGVREGVPKMALVLTDGHS 367 HSP 2 Score: 62.003 bits (149), Expect = 3.313e-9 Identity = 84/374 (22.46%), Postives = 164/374 (43.85%), Query Frame = 3
Query: 2697 LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR----EDVPKVLVVLTDG-ANDIGESPQISAV-KAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNA---KTISNEACQIPQKPTICKAAG---DLIFVMGSSANKTSERFKQMSDFM--KSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDM---------TTSTNGVFIGKALTSARVLFKSD-----TRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGK--TSESDLKSVASPDKALF 3728
LD++ +D S SL + FQ E+D + RI++++ + K TR+ + F+ +L + + ++ ++ + + G T IG A A L T G VPK++ +L+DG +D + Q++ + + ++ I I + G G ++ L + S + + T +L+K K E C+ Q+P+ K + DL V+ +S + F F+ + L + + E RVA++ ++ ++ F +S++ TT+TN AL A L S R+ K +++ DG + E ++ GI + + +T + E++L+ +A +K F
Sbjct: 53 LDLVFILDSSGSLR-DKFQDEIDIIRRILKHVTIGKTATRVMLIQFSGTQHLEFNFEKFTDREELLAALDVLRHVSGITRIGGAFEFALQQLKTPGSGLRDGTVPKIVYLLSDGRTHDFPKDWQMADILRREIPNIDIWAYGTGDYVAMAALANYTQSEQ--KIVTNLNLQKLEPQFEKYHGTEICE--QQPSCVKGSDKPLDLALVVDASES-LDHLFSDQKKFLVERVLGNIN-IHPEAVRVALITYSGQAFVHFKFNSFLYGNNTSVQGFVKNIRSIKGTTATN-----VALMDAFDLLTSKDPSIGVREGVPKMALVLTDGHSHKSPKDISEK--MRAAGIIMIAVSVTPRPLVDEAELRLIAGSEKRAF 412 HSP 3 Score: 61.6178 bits (148), Expect = 3.927e-9 Identity = 41/139 (29.50%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 3759 NAYISSELCDVIKGTNKECS--TKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGE----ALRECKNELLNSTRKEVPKVIVLVTDG 4157
N E+ T C LDL+F LDSS S+ D +Q E+D + RI++ + I T+V ++ F+ EF ++FT++ +++A+ + G T IG AL++ K VPK++ L++DG
Sbjct: 28 NGLAPPEIVHTPVSTKPRCKVFAPPLDLVFILDSSGSLR-DKFQDEIDIIRRILKHVTIGKTATRVMLIQFSGTQHLEFNFEKFTDREELLAALDVLRHVSGITRIGGAFEFALQQLKTPGSGLRDGTVPKIVYLLSDG 165 HSP 4 Score: 51.6026 bits (122), Expect = 5.027e-6 Identity = 45/161 (27.95%), Postives = 78/161 (48.45%), Query Frame = 3
Query: 960 EISTPDQNC---NNQQLDLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGS--KQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-----REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGL 1409
EI +C +++ LDL V+DAS SL L+ DQ+ V+ ++ I + +V ++ YS + S N T +Q FV + ++G T + AL + + SK REG K +++TDG + P+ +EK++ GI +I + +
Sbjct: 233 EICEQQPSCVKGSDKPLDLALVVDASESLDHLFSDQKKFLVERVLGNINIHPEAVRVALITYSGQAFVHFKFNSFLYGNNTSVQGFVKNIRSIKGTTATNVALMDAFDLLTSKDPSIGVREGVPKMALVLTDGHSHK---SPKDISEKMRAAGIIMIAVSV 390
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Match: mua-3 (Transmembrane cell adhesion receptor mua-3 [Source:UniProtKB/Swiss-Prot;Acc:P34576]) HSP 1 Score: 63.929 bits (154), Expect = 1.129e-9 Identity = 52/195 (26.67%), Postives = 96/195 (49.23%), Query Frame = 3
Query: 3813 CSTKQLDLMFALDSSSSIGPDYWQTE-LDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTR------KEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLK-ILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKK 4373
C ++ DL+F +D S SIG ++ E L FV V +I + T+VG++ ++ R EF LDQ+ ++ +++ + + G T G A++ E + R ++ +V +++TDG S ++ +S +KL I IG+ D S+L+ I S ++ + FK L + I + +C + K
Sbjct: 640 CPKQKTDLVFLIDGSGSIGSYVFKNEVLRFVREFVELFEIGRSKTRVGLIQYSDQIRHEFDLDQYGDRDSLLKGISETQYLTGLTRTGAAIQHMVQEGFSERRGARPQQSDIARVAIILTDGRSQDNVTGPADSA--RKLSINTFAIGVTDHVLASELESIAGSPNRWFYVDKFKDLDTRLRSMIQKAACPSPTK 832 HSP 2 Score: 59.6918 bits (143), Expect = 1.939e-8 Identity = 45/193 (23.32%), Postives = 95/193 (49.22%), Query Frame = 3
Query: 1509 DIAQDACQIPKPKRICSA-------KLDIALIVDCSSQVQLHEWVNQLL-FAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHI--YDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK-------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQD 2036
D++ +A +P P R+C+ K D+ ++D S + + + N++L F + F+E FE+G +KTR L+ ++++ E + D +LK + +++ G TR A+ + + + + ++ R+ ++LT+G S + V A ++ ++ IG+ D EL+ ++ PN + D
Sbjct: 621 DVSSNA-NLP-PGRVCTVQTTCPKQKTDLVFLIDGSGSIGSYVFKNEVLRFVREFVELFEIGRSKTRVGLIQYSDQIRHEFDLDQYGDRDSLLKGISETQYLTGLTRTGAAIQHMVQEGFSERRGARPQQSDIARVAIILTDGRSQDNVTG--PADSARKLSINTFAIGVTDHVLASELESIAGSPNRWFYVD 809
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 196.438 bits (498), Expect = 6.402e-50 Identity = 226/911 (24.81%), Postives = 413/911 (45.33%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTT---------PAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAA--GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGV----FIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL--NSTRKE--VPKVIVLVTDGVST 4166
DI IVD S + + + F R I E+ + R +V + + E + F + +DI+ ++ + + GG T AL A + + + + S K++ +++T+G S + V T A +L+ SGV + +G+ + A EELK + ++P F F SL + K CK + Q C + DI F++D S +T ++ ++ +L +LQ NI R+ ++ T +F L E + A++ + G + AL +F K A V +I+++ TDK T+Q D AE L+ GV + +G+ S + A + + N + ++ K +I IC Q+ C K D++ ID S+S+ +F F+ ++V + +D +G+ F++ P++ F+ ++++ I+ I + G+T G A+ + Y GR DVP+ LVV+TDG A+D P A++ K G+ I S+GV ++ +L +I+ + + Y + F L + K + + C P C + D+IF++ + + F+++ F+ S+ T + R ++ ++ S F + N I + TS + +AL + F ++ + + +I DG+ DR A+ I V+ IG+ + +S+L+ + S +K V+N+ NLQ +S LC+ K C + DL+F +D S SI + W T + F+ +V + I +VG+ F++ +KEF ++++ + + SA++RI T IG+AL +RK+ VP+ +VL+TDGVS+
Sbjct: 416 DIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFAN-KNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGESEDDVTTTAA--ELRRSGVTVYALGVKN-ASVEELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNS-GSTRADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVASLFNQAKASRPAKVQEILIVITDK--TSQDDVGDPAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNYDALNVLKREIVTDICS-----QEACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDGEAHDAVAVPA-KAIRDK--GVTIFSIGVAS-VNTTQLWEISGTQDKVYVQRDFDALHSID-KNLQFKLCS--SHPGGCPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVYSD---TPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFAAKQINVYGIGVA-RAQDSELEIITKSKNKIFHVNNYDNLQDLQMNVSGVLCNATKPV---CQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFSSVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSS 1300 HSP 2 Score: 143.28 bits (360), Expect = 1.171e-33 Identity = 239/1126 (21.23%), Postives = 467/1126 (41.47%), Query Frame = 3
Query: 990 NQQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFD--TTDIAQDACQIPK--PKRICSAKLDIALIVDCSSQ---VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLK----HVGGFTRIDKALNLASRSFYLK------SKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQT-------CSSN--GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQR-----------QDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVP----KVLVVLTD--GANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF--NRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT--RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRI----IFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDG 4157
N D++F++D+SSS+ +E+ F + + L+ID K QVGV+ +S LG NK E+ + + L G T++ A+ + + S+ E + V+++TDG P AE L+K GI + IG+G S +L ++ST + ++ TT + + C + + K + D+ ++VD ++Q Q+ E +N+LL Q +G + +L F E VV+E Y K E + + G ++ KA++ +F +K K+ +++L G S + T++A ++ V + I R E + S FP+ LR + + + V+ T I+ + C S DI+F+VD+S T ++ I+ + + L ++ + R+ ++ Y+D + EF LD K DI IK + G G AL A+ R V +I ++ TD ++ D + A L+ GV + +G+ KN+ + + S + N+ K++ +C R Q ++ D+ +D S S +F+ F+ +Q ++ +R R+G+ + L + + A + + I G + G A+ LF + + P ++L+V+TD +D+G+ A + ++ G+ + ++GV ++ L A + + V + L + + S EAC+ D++F++ S++ F M F+ + T++ +++ V +++F N D E +I+ + T + GKAL+ F + R + + +++I DGE D A ++ G+ +F+IG+ + + + DK +F L + + +LC G C + QL D++F + + I ++ F+ +V I N + GV+ + +D +F L+Q+ + + A+ + + A + +L ++ VP+++ ++TDG
Sbjct: 31 NPPYDIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDDIKGP---AEDLRKKGIIIFAIGVG-STNMTELKAIASTPPERFVVNIDNYQALQGLTTTMKETVCVLMRDQGKALAPKFTDVFVLVDSTAQGETQQIRELLNRLLV------QLNVGDGSNKIALAQFG-ENVVKEFLFKDYQTAQKAREFINRFEPRLNGERKLGKAIDYVRTNFLNTVSGSRIAKGYKQYLLVLRTGDSYD--STVRAIRTMKNEDVSVIDI-------RLEPNLQSLFPS--LR-----AFQIDQNVIDVAADVTKRIQEKEVFNVTGDCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDG--ESEDDVTTTAAELRRSGVTVYALGV-KNASVEELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNSGST-RADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVAS-LFNQAKASRPAKVQEILIVITDKTSQDDVGD----PAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNYDALNVLKREIVTDICSQEACK--------NKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDGEAHDAVAV--PAKAIRDKGVTIFSIGVASVNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSSHPGG---CPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVY-SDTPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDG 1106 HSP 3 Score: 121.324 bits (303), Expect = 5.741e-27 Identity = 210/1018 (20.63%), Postives = 436/1018 (42.83%), Query Frame = 3
Query: 981 NCNNQQL-DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGRE-----GAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD--IAQDACQIPKPKR-------------ICSAKLDIALIVDCSSQVQL-HEWVNQLLFAKRFIEQFELGINKTR-------------FSLVSFANEAVVEEEFTHIYDDI--LKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN-GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEIN------KIPFIEGNTA--IGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQ-QISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDS 3854
+C + Q+ D++F++D S S+ ++ F + +I L+++ +VG+++Y+D L + NK +I ++ + G T AL+ + ++ R G K+ I++TDG A +L++ G+ V +G+ + + ++L K+ S + + ++ + + + + + C++ +R + + + DI ++D S + E V + + ++ F +G N+ R FSL N A E I + +K ++LK+V N A S + + +++++T+ S + V A +L+ GV + IG+ D + E LK+ + F ++ L + V C + C + DI+F++D S+ + +++ + + I + + ++ +S+ +F L+ F + ++ AI + AL+ + F R DV + +V+ TD D+++ A+ ++ GV I +G++ + +E ++ + + + + ++ +C + C +L DVI + + + ++F+ F+ +V + + + R G+ VY + P + + +++I K P + G TA + +++ T+ + + VP++L ++TDG E+ + A + I + +GV + Q SE E+ S + ++V + +L+ +S C KP DL+F++ S + + E + + F+ ++ + E RV I +F+ Y + +YK+ +S+I +T G IGKAL++ F+ D +RK + + +++I DG +DR E A+ L+ GI VF IG+ G S L +A SPD+ V NF+ L A LC + C+ +D+ D+
Sbjct: 408 DCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGESEDDVT---TTAAELRRSGVTVYALGVKNASV-EELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNSGSTRADFEDVKKFILGS--LQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVASL------FNQAKAS---RPAKVQEILIVITDKTSQDDVG--DPAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNYDALNVLKREIVTDICSQ------EACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDG---EAHDAVAVPAKAIRDKGVTIFSIGVASVNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSS---HPGGCPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVI-----VYSDTPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFAAKQINVYGIGVARAQDSELEI-ITKSKNKIFHVNNYDNLQDLQM-NVSGVLCN-ATKPVCQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFS-SVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSSDR--VNEAADQLRNLGINVFAIGI-GAVSLQQLSYIAGSPDRLFKVQNFNYLNLTTATFVDALCVPLPLAQASCT---IDIGIGFDN 1381 HSP 4 Score: 113.62 bits (283), Expect = 1.179e-24 Identity = 122/514 (23.74%), Postives = 242/514 (47.08%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQI--PQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESS---IEDMTTSTNGVF-IGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDV-IKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVS 4163
D++ +D S+S+G +F+ F++ V L++ + ++G+ F+++ + + A++ ++I+ +P+ G T +G A+ D FT E VP+V+VV+TDG D + + A + GI I ++GVG + EL IAS+ N+ + L+ T+ C + Q + D+ ++ S+A +++ +++ + + L +L V D N ++A+ +F N + +DY+ + + I NG +GKA+ R F S K + ++++ G+ D T +K + + V I L E +L+S+ +A +D N+ A ++ + + + +C + Q+ D++F +D S SI + ++ F+ RI+ +++N++ +VG+V +N EF LD F K +++ +K I + G T G AL+ +N L R V ++ +++TDG S
Sbjct: 35 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDG--DSTDDIKGPAEDLRKKGIIIFAIGVGS-TNMTELKAIASTPPERFVVNIDNYQALQGLTT-TMKETVCVLMRDQGKALAPKFTDVFVLVDSTAQGETQQIRELLN--RLLVQLNVGDGSN-KIALAQFGE-NVVKEFLFKDYQTAQKAREFINRFEPRLNGERKLGKAIDYVRTNFLNTVSGSRIAKGYKQYLLVLRTGDSYD--STVRAIRTMKNEDVSVIDIRL-----EPNLQSLFPSLRAFQID--QNVIDVAADVTKRIQEKEVFNVTGDCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGES 531 HSP 5 Score: 68.5514 bits (166), Expect = 7.162e-11 Identity = 123/560 (21.96%), Postives = 246/560 (43.93%), Query Frame = 3
Query: 966 STPDQNCNNQQL-DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI--SKGRE--GAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF-THIY---DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLR-QDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIW-----------DGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDE-AFTKRDIYVAIKQLR-SNGNGGDLNTALNRARQVFKIDYRAD--VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIF 2528
S+ C + QL D+IF++ + + L +++ +F +V+ S QI + GV++YSD L + +I + ++ L+ + + + AL S F + GR+ G + + ++TDG D A++ I V IG+ L +I NK+ Y ++D+ +++ C KP +C ++ D+ ++D S + W+ + F +++ +G R + F+ +V ++EF + Y D + + + + T I KAL+ F+ + K S++ +VL+T+G+S +RV +AA L+ G+++ IG+G ++L ++ P+ + Q+F V C P + +C+ + I F +++ ++ I+ + IRS+ + + +L + + I + T RLD+ +F + D+ V K + N L R+ F+ +RA K++++FTD L ++ LS L+ +GVK +
Sbjct: 982 SSHPGGCPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVYSDT-PSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDG-DARDRENLRARADEFAAKQINVYGIGVARAQDSELEIITKSKNKIFHVNNYDNLQDLQ-------MNVSGVLCNATKP--VCQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFS--SVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVE-FFQEDKGSRKQSGVPQNLVLITDGVSSDRVN--EAADQLRNLGINVFAIGIG-AVSLQQLSYIAGSPDRLFKVQNFNYLNLTTATFVDALCVPLP-LAQASCTIDIGIGFDNSRTSSSSRSIFRDQMKLQTYLPEIIRSI--SEVHNLCCITKPTIKTNIGFRLVDATGKRLDDFSFEEYDVGVLNKVMALQTSQSLKFNNLLLRS---FEEKFRASNSFVKVLIIFTDGLDEPVENLLSTVNSLRTNGVKALL 1518 HSP 6 Score: 67.781 bits (164), Expect = 1.325e-10 Identity = 39/117 (33.33%), Postives = 66/117 (56.41%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST-----RKEVPKVIVLVTDGVST 4166
D++F +DSSSSIG ++ F+ V ++I+ +VGVV F+TD KEF L ++ K + + + + G T +G+A+ K+ S ++VP+V+V++TDG ST
Sbjct: 35 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDST 151 HSP 7 Score: 55.0694 bits (131), Expect = 7.694e-7 Identity = 46/155 (29.68%), Postives = 82/155 (52.90%), Query Frame = 3
Query: 114 LALDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVR-LHRKVGGDSSVLGRALN--STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
L L Q C+ + DI +LD+S S T +F+ I L F I PN RV + +F T+H + + AVR + + VGG S G+AL ++L++ RP +++ IL++++D ++ D+ V D A +++ +GV++Y + +
Sbjct: 609 LLLKQGCVNTEEADIYFLLDNSGS-TRADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGG--SEKGKALKYVASLFNQAKASRP-AKVQEILIVITDKTSQDD--VGDPAEELRIQGVSVYAIGV 757 HSP 8 Score: 52.373 bits (124), Expect = 6.544e-6 Identity = 43/188 (22.87%), Postives = 93/188 (49.47%), Query Frame = 3
Query: 144 PRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAV-RLHRKVGGDSSVLGRALN---STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXX--KKNVVMFPEFQTMRGDSENLVSVICVV 689
P DI ++DSSSSI +F+ ++ F+ I +V + + EFL ++ + L + L + GG + +G+A+N + R + ++ +++++++DG +TD+ +K A +++KG+ I+ + +GS L S+ ++ VV +Q ++G + + +CV+
Sbjct: 33 PYDIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGG--TYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDD--IKGPAEDLRKKGIIIFAIGVGSTNMTELKAIASTPPERFVVNIDNYQALQGLTTTMKETVCVL 216 HSP 9 Score: 51.9878 bits (123), Expect = 6.884e-6 Identity = 48/214 (22.43%), Postives = 99/214 (46.26%), Query Frame = 3
Query: 126 QRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALN--STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVR 761
+ C K DI ++D SSSI +F S I + ++ +I ++ V + + + + +F ++ L A+ ++ GD+ G+AL+ S +D RP ++ + L++++DG D ++V A I++KGVTI+++ + S L ++ V + +F + +NL +C PS + + + + +R
Sbjct: 799 EACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYT-GKALSFISKYFDASNGGRP--DVPQFLVVITDGEAHDAVAVP--AKAIRDKGVTIFSIGVASVNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSSHPGGCPSTQLADVIFLVQCTRQIR 1007
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 196.438 bits (498), Expect = 8.476e-50 Identity = 226/911 (24.81%), Postives = 414/911 (45.44%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTT---------PAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAA--GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGV----FIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKE--VPKVIVLVTDGVST 4166
DI IVD S + + + F R I E+ + R +V + + E + F + +DI+ ++ + + GG T AL A + + + + S K++ +++T+G S + V T A +L+ SGV + +G+ + A EELK + ++P F F SL + K CK + Q C + DI F++D S +T ++ ++ +L +LQ NI R+ ++ T +F L E + A++ + G + AL +F K A V +I+++ TDK T+Q D AE L+ GV + +G+ S + A + + N + ++ K +I IC Q+ C K D++ ID S+S+ +F F+ ++V + +D +G+ F++ P++ F+ ++++ I+ I + G+T G A+ + Y GR DVP+ LVV+TDG A+D P A++ K G+ I S+GV ++ +L +I+ + + Y + F L + K + + C P C + D+IF++ + + F+++ F+ S+ T + R ++ ++ S F + N I + TS + +AL + F ++ + + +I DG+ DR A+ I V+ IG+ + +S+L+ + S +K V+N+ NLQ +S LC+ K C + DL+F +D S SI + W T + F+ +V + I +VG+ F++ +KEF ++++ + + SA++RI T IG+AL + +RK+ VP+ +VL+TDGVS+
Sbjct: 391 DIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFAN-KNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGESEDDVTTTAA--ELRRSGVTVYALGVKN-ASVEELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNS-GSTRADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVASLFNQAKASRPAKVQEILIVITDK--TSQDDVGDPAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNYDALNVLKREIVTDICS-----QEACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDGEAHDAVAVP-AKAIRDK--GVTIFSIGVAS-VNTTQLWEISGTQDKVYVQRDFDALHSID-KNLQFKLCS--SHPGGCPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVYSD---TPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFAAKQINVYGIGVA-RAQDSELEIITKSKNKIFHVNNYDNLQDLQMNVSGVLCNATKPV---CQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFSSVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSS 1275 HSP 2 Score: 142.895 bits (359), Expect = 1.689e-33 Identity = 240/1128 (21.28%), Postives = 469/1128 (41.58%), Query Frame = 3
Query: 990 NQQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFD--TTDIAQDACQIPK--PKRICSAKLDIALIVDCSSQ---VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLK----HVGGFTRIDKALNLASRSFYLK------SKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQT-------CSSN--GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQR-----------QDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVP----KVLVVLTD--GANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE----DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT--RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRI----IFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDG 4157
N D++F++D+SSS+ +E+ F + + L+ID K QVGV+ +S LG NK E+ + + L G T++ A+ + + S+ E + V+++TDG P AE L+K GI + IG+G S +L ++ST + ++ TT + + C + + K + D+ ++VD ++Q Q+ E +N+LL Q +G + +L F E VV+E Y K E + + G ++ KA++ +F +K K+ +++L G S + T++A ++ V + I R E + S FP+ LR + + + V+ T I+ + C S DI+F+VD+S T ++ I+ + + L ++ + R+ ++ Y+D + EF LD K DI IK + G G AL A+ R V +I ++ TD ++ D + A L+ GV + +G+ KN+ + + S + N+ K++ +C R Q ++ D+ +D S S +F+ F+ +Q ++ +R R+G+ + L + + A + + I G + G A+ LF + + P ++L+V+TD +D+G+ A + ++ G+ + ++GV ++ L A + + V + L + + S EAC+ D++F++ S++ F M F+ + T++ +++ V +++F+ N F D E +I+ + T + GKAL+ F + R + + +++I DGE D A ++ G+ +F+IG+ + + + DK +F L + + +LC G C + QL D++F + + I ++ F+ +V I N + GV+ + +D +F L+Q+ + + A+ + + A + +L ++ VP+++ ++TDG
Sbjct: 6 NPPYDIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDDIKGP---AEDLRKKGIIIFAIGVG-STNMTELKAIASTPPERFVVNIDNYQALQGLTTTMKETVCVLMRDQGKALAPKFTDVFVLVDSTAQGETQQIRELLNRLLV------QLNVGDGSNKIALAQFG-ENVVKEFLFKDYQTAQKAREFINRFEPRLNGERKLGKAIDYVRTNFLNTVSGSRIAKGYKQYLLVLRTGDSYD--STVRAIRTMKNEDVSVIDI-------RLEPNLQSLFPS--LR-----AFQIDQNVIDVAADVTKRIQEKEVFNVTGDCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDG--ESEDDVTTTAAELRRSGVTVYALGV-KNASVEELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNSGST-RADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVAS-LFNQAKASRPAKVQEILIVITDKTSQDDVGD----PAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNYDALNVLKREIVTDICSQEACK--------NKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFS--NNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITDGEAHD--AVAVPAKAIRDKGVTIFSIGVASVNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSSHPGG---CPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVY-SDTPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDG 1081 HSP 3 Score: 120.553 bits (301), Expect = 1.207e-26 Identity = 206/1010 (20.40%), Postives = 436/1010 (43.17%), Query Frame = 3
Query: 981 NCNNQQL-DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGRE-----GAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD--IAQDACQIPKPKR-------------ICSAKLDIALIVDCSSQVQL-HEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK-----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN-GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEIN------KIPFIEGNTA--IGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQ-QISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDS 3854
+C + Q+ D++F++D S S+ ++ F + +I L+++ +VG+++Y+D L + NK +I ++ + G T AL+ + ++ R G K+ I++TDG A +L++ G+ V +G+ + + ++L K+ S + + ++ + + + + + C++ +R + + + DI ++D S + E V + + ++ F +G N+ R +V ++ T + V + G + KAL + S + ++K S+ +++++T+ S + V A +L+ GV + IG+ D + E LK+ + F ++ L + V C + C + DI+F++D S+ + +++ + + I + + ++ +S+ +F L+ F + ++ AI + AL+ + F R DV + +V+ TD D+++ A+ ++ GV I +G++ + +E ++ + + + + ++ +C + C +L DVI + + + ++F+ F+ +V + + + R G+ VY + P + + +++I K P + G TA + +++ T+ + + VP++L ++TDG E+ + A + I + +GV + Q SE E+ S + ++V + +L+ +S C KP DL+F++ S + + E + + F+ ++ + E RV I +F+ Y + +YK+ +S+I +T G IGKAL++ F+ D +RK + + +++I DG +DR E A+ L+ GI VF IG+ G S L +A SPD+ V NF+ L A LC + C+ +D+ D+
Sbjct: 383 DCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGESEDDVT---TTAAELRRSGVTVYALGVKNASV-EELKKIGSYPEHEFVFNVGSFLMLSSLEKSLRKSLCKVVVNRRFDKNNRLLLKQGCVNTEEADIYFLLDNSGSTRADFEDVKKFILGS--LQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGGSEKGKALKYVA-SLFNQAKASRPAKVQEILIVITDKTSQDDVG--DPAEELRIQGVSVYAIGVKDASQDELLKMTADETKDFYVTNYDALNVLKREIVTDICSQ------EACKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYTGKALSFISKYFDASNGGRPDVPQFLVVITD---GEAHDAVAVPAKAIRDKGVTIFSIGVASVNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSS---HPGGCPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVI-----VYSDTPSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDGDARDRENLRARADEFAAKQINVYGIGVARAQDSELEI-ITKSKNKIFHVNNYDNLQDLQM-NVSGVLCNA-TKPVCQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFS-SVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVVEFFQEDKGSRKQSGVPQNLVLITDGVSSDR--VNEAADQLRNLGINVFAIGI-GAVSLQQLSYIAGSPDRLFKVQNFNYLNLTTATFVDALCVPLPLAQASCT---IDIGIGFDN 1356 HSP 4 Score: 113.62 bits (283), Expect = 1.429e-24 Identity = 122/514 (23.74%), Postives = 242/514 (47.08%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQI--PQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESS---IEDMTTSTNGVF-IGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDV-IKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVS 4163
D++ +D S+S+G +F+ F++ V L++ + ++G+ F+++ + + A++ ++I+ +P+ G T +G A+ D FT E VP+V+VV+TDG D + + A + GI I ++GVG + EL IAS+ N+ + L+ T+ C + Q + D+ ++ S+A +++ +++ + + L +L V D N ++A+ +F N + +DY+ + + I NG +GKA+ R F S K + ++++ G+ D T +K + + V I L E +L+S+ +A +D N+ A ++ + + + +C + Q+ D++F +D S SI + ++ F+ RI+ +++N++ +VG+V +N EF LD F K +++ +K I + G T G AL+ +N L R V ++ +++TDG S
Sbjct: 10 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDG--DSTDDIKGPAEDLRKKGIIIFAIGVGS-TNMTELKAIASTPPERFVVNIDNYQALQGLTT-TMKETVCVLMRDQGKALAPKFTDVFVLVDSTAQGETQQIRELLNRL--LVQLNVGDGSN-KIALAQFGE-NVVKEFLFKDYQTAQKAREFINRFEPRLNGERKLGKAIDYVRTNFLNTVSGSRIAKGYKQYLLVLRTGDSYD--STVRAIRTMKNEDVSVIDIRL-----EPNLQSLFPSLRAFQIDQ--NVIDVAADVTKRIQEKEVFNVTGDCRSAQVADIVFIVDESGSITRNNFELIKRFLHRIISGLEVNSDSVRVGMVLYNDRPSAEFYLDTFANKNDIMNYIKIIPYRGGGTATGAALKFAQNNLFTQKRGSRKALGVKQIAIVMTDGES 506 HSP 5 Score: 67.781 bits (164), Expect = 1.368e-10 Identity = 39/117 (33.33%), Postives = 66/117 (56.41%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST-----RKEVPKVIVLVTDGVST 4166
D++F +DSSSSIG ++ F+ V ++I+ +VGVV F+TD KEF L ++ K + + + + G T +G+A+ K+ S ++VP+V+V++TDG ST
Sbjct: 10 DIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGGTYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDST 126 HSP 6 Score: 67.0106 bits (162), Expect = 2.325e-10 Identity = 123/560 (21.96%), Postives = 246/560 (43.93%), Query Frame = 3
Query: 966 STPDQNCNNQQL-DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI--SKGRE--GAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF-THIY---DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLR-QDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIW-----------DGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDE-AFTKRDIYVAIKQLR-SNGNGGDLNTALNRARQVFKIDYRAD--VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIF 2528
S+ C + QL D+IF++ + + L +++ +F +V+ S QI + GV++YSD L + +I + ++ L+ + + + AL S F + GR+ G + + ++TDG D A++ I V IG+ L +I NK+ Y ++D+ +++ C KP +C ++ D+ ++D S + W+ + F +++ +G R + F+ +V ++EF + Y D + + + + T I KAL+ F+ + K S++ +VL+T+G+S +RV +AA L+ G+++ IG+G ++L ++ P+ + Q+F V C P + +C+ + I F +++ ++ I+ + IRS+ + + +L + + I + T RLD+ +F + D+ V K + N L R+ F+ +RA K++++FTD L ++ LS L+ +GVK +
Sbjct: 957 SSHPGGCPSTQLADVIFLVQCTRQIRLQDFEKIKSFLISVVNSTQIGDNLIRFGVIVYSDT-PSQFSLNQYNTRRQIAEAITSLKSPAVSGYTARALAYSLTYFTEANGGRQKRGVPQMLFMITDG-DARDRENLRARADEFAAKQINVYGIGVARAQDSELEIITKSKNKIFHVNNYDNLQDLQ-------MNVSGVLCNATKP--VCQNEVADLVFLIDGSESISEESWITVIAFLLNVVDKLRIGPELFRVGIAQFS--SVYQKEFYMNEYKDADGVKSAIRRITQIKEGTLIGKALSNVV-EFFQEDKGSRKQSGVPQNLVLITDGVSSDRVN--EAADQLRNLGINVFAIGIG-AVSLQQLSYIAGSPDRLFKVQNFNYLNLTTATFVDALCVPLP-LAQASCTIDIGIGFDNSRTSSSSRSIFRDQMKLQTYLPEIIRSI--SEVHNLCCITKPTIKTNIGFRLVDATGKRLDDFSFEEYDVGVLNKVMALQTSQSLKFNNLLLRS---FEEKFRASNSFVKVLIIFTDGLDEPVENLLSTVNSLRTNGVKALL 1493 HSP 7 Score: 55.4546 bits (132), Expect = 7.448e-7 Identity = 46/155 (29.68%), Postives = 82/155 (52.90%), Query Frame = 3
Query: 114 LALDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVR-LHRKVGGDSSVLGRALN--STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
L L Q C+ + DI +LD+S S T +F+ I L F I PN RV + +F T+H + + AVR + + VGG S G+AL ++L++ RP +++ IL++++D ++ D+ V D A +++ +GV++Y + +
Sbjct: 584 LLLKQGCVNTEEADIYFLLDNSGS-TRADFEDVKKFILGSLQLFNIGPNRVRVGVVKVDRNPTLQFSLTEHKNRASFEAAVRGISQPVGG--SEKGKALKYVASLFNQAKASRP-AKVQEILIVITDKTSQDD--VGDPAEELRIQGVSVYAIGV 732 HSP 8 Score: 52.373 bits (124), Expect = 6.754e-6 Identity = 43/188 (22.87%), Postives = 93/188 (49.47%), Query Frame = 3
Query: 144 PRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAV-RLHRKVGGDSSVLGRALN---STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXX--KKNVVMFPEFQTMRGDSENLVSVICVV 689
P DI ++DSSSSI +F+ ++ F+ I +V + + EFL ++ + L + L + GG + +G+A+N + R + ++ +++++++DG +TD+ +K A +++KG+ I+ + +GS L S+ ++ VV +Q ++G + + +CV+
Sbjct: 8 PYDIVFLVDSSSSIGTRDFREVKTFMHTFVDGLEIDTKKVQVGVVQFSTDPHKEFLLGEYANKAELFRKIDNLPYRTGG--TYMGKAMNFLKDNYFTSAGGSRVSEKVPQVVVVITDGDSTDD--IKGPAEDLRKKGIIIFAIGVGSTNMTELKAIASTPPERFVVNIDNYQALQGLTTTMKETVCVL 191 HSP 9 Score: 51.6026 bits (122), Expect = 9.221e-6 Identity = 48/212 (22.64%), Postives = 98/212 (46.23%), Query Frame = 3
Query: 132 CIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALN--STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVR 761
C K DI ++D SSSI +F S I + ++ +I ++ V + + + + +F ++ L A+ ++ GD+ G+AL+ S +D RP ++ + L++++DG D ++V A I++KGVTI+++ + S L ++ V + +F + +NL +C PS + + + + +R
Sbjct: 776 CKNKVADIMFLIDGSSSIYGPDFTSMKTFITKVVNGTIIGEDSVHVGVVQFSNNPQEQFPLNRYFDQNELEEAIDGIEQLTGDTYT-GKALSFISKYFDASNGGRP--DVPQFLVVITDGEAHDAVAVP--AKAIRDKGVTIFSIGVASVNTTQLWEISGTQDKVYVQRDFDALHSIDKNLQFKLCSSHPGGCPSTQLADVIFLVQCTRQIR 982
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: CR545476.3 (pep chromosome:GRCz11:CHR_ALT_CTG9_1_11:23915065:23940181:-1 gene:ENSDARG00000114020.1 transcript:ENSDART00000190370.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR545476.3) HSP 1 Score: 142.51 bits (358), Expect = 2.617e-33 Identity = 295/1237 (23.85%), Postives = 530/1237 (42.85%), Query Frame = 3
Query: 960 EISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI------SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG--------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQ---DFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNG----------DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNT--ALNRAR-QVFKIDYRA----DVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXX-----XXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTF----NSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKGR-----EDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTI-------SNEACQI-PQKPT---ICKAAGD---LIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTS--------TNGVFIGKALTS-ARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL----------DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALREC-KNELLNSTR---KEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRES 4355
E STP + D++F+LD S + F V++ I+ +V V+ YS E+ L S K +I V+ L G + A + + + S+ EG + +IL++ G + A LK++G V+ +G+G L I + N S + + ++ + L +A A + + + D+ ++D S + + + F +R +EQ + N+ R S+V ++ +A V + + Y D+L V L+H GG AL S + S S++ +++LL+ G S + V T +A L+ESGV I +G+G + E++ ++ +P Q DF + + ++ +KP T + G D++F++D S D T + +R + ++ LN+S R+S++ YS + F L+ K D I+ LR G G LNT AL R VF + V ++++L + + D+ A LK GV I +G S++S + ++++ S N K I + + F E K D+I ID S +G E F I +FV R+V NL++ +++ RIG+ + N+++YLN S+ + I ++ + G +G AI + GR E VP+ +VV++ G A D + + SA K GI +S+G + + +EL + F Y V L I S E Q+ P PT + GD ++F++ ++ K F + D ++ + + V +N RV++++ Y D E N S+ E++ + N + GKAL ++ +++ S + + +I++ G+ D + AN LK + +G +E +L+ ++ SP+ A + +F L + L V T E ST + D++F +D S S+G DF+ ++V +D+ + +V +V + + EF L+ K ++ISA+KR+ G D+ EA++ +NEL S + + +V++T G ST +S +++K + I IG ++ S ++I +SS ++ F L + FI R S
Sbjct: 8 EYSTPRK-------DVVFLLDGSDGTRSSFPAMRDFVQRVVEKFNIEANRDRVSVVQYSRDAEVHFYLNSYTKKEDILDRVTGLRHKGGRPLYTGAALQYVRDNVFTASSGSRRLEGVPQILILLSGGRSFDSV---DAAASSLKELG--VLTLGIGSRGSDSRELQRISYEANYALSVADFSELPNVQEQLLASVQTVAMSAGPLLTTYIVNEVVIKDVVFLLDGSDGTR-NTFPAMRAFVQRLVEQLNVEANRDRISVVQYSRDAEVNF-YLNTYTAKADVLNTVRGLRHRGGRPLNTGAALQYVRDSVFTASAGSRKQEGIPQVLILLSGGRSNDNVDTPASA--LKESGVLI--LGIGTRNSSREVQRITN--DPAYAQSLNDFSDLPGVQQQFYSSLNNVLVQVKPMTPTVAGKTEFPRMARRDVVFLLDGS-DGTRSSFSAMRDFVERMVERLNVSENRDRVSVVQYSRDPEAHFYLNTYSRKEDALDTIRGLRHKG-GRPLNTGAALQYLRDNVFTASSGSRRVEGVPQLLILLSGTRSFDNVDT--PASSLKELGVLIFAIG-SRSSDSQELQRVSNEPSYALSVREFSDLSNVQQKLLSNINTALVEVFLSFLVESEGPKKDIIFLIDGSEDVGRE-FPIIQEFVRRVVSNLNVGENKIRIGVVQYGDIPNADIYLN----SHRTKEGVLNGIKELRQLRGTKRNLGQAIDFVRREVLASGRGGRKEEGVPQFVVVVSGGKATD---NIRQSATALKQSGIFPLSIGT-RDVGTQELQVTSYVPRFAYLVDDLPGLYTIQETLINTLTELSSEELAQLRPVYPTETVVPVPRGDKRDVVFLIDGTS-KIRNEFPAIRDMVQRVVEKLDVGLDNVRVSVVQ-----YSDDPKLEFLLNEHSTKEEVRQAIRRLRNKGGNELNTGKALEYVSKTIYQRSAGSRVEEGVPQFLILVTGGKSND--DVSGPANQLKLSRVAPLAVGARDADAE-ELRLISFSPELAYTIRDFQQLPGVEQQL---LTKVSTMTRDEISTPAVPRDPLNLGRKDIIFLIDGSDSVGQSGVAHIRDFILKVVDQLDVRPDQVRVALVQYGERPKTEFSLNSHDNKQSVISAIKRLRHMGGRGADLAEAIKYVIRNELQASAGVRLAQASQHLVVLTGGRSTSDVS--TYGSILKGSRVNCIGIGAENADSRQLIQIATSSDDVLQVPSFPNLPNIQNKFIARLS 1196 HSP 2 Score: 87.0409 bits (214), Expect = 1.784e-16 Identity = 112/547 (20.48%), Postives = 248/547 (45.34%), Query Frame = 3
Query: 2661 FKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAI-GWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISE-KELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG--------------DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFI--GKAL-TSARVLFKSDT---------RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNL----QRYNAYISSELCDV------IKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALRECKNELL----NSTRKE-VPKVIVLVT 4151
F E + + DV+ +D S +F DFV R+V+ ++ +R R+ + ++ + ++ ++S + D+ + + G G A+ D +FT + E VP++L++L+ G + + A + L + ++++G+G + S+ +EL I+ + +V F++L + +++ T+ +AG D++F++ S + T F M F++ L + V+ R+++++++R ++ Y N ++ D+ + G+ G+ L T A + + D+ ++ V+I++ G ++ N A+ LK+ G+ + IG T +S + P A +++FS+L Q++ + +++ L V + G + + D++F LD S + DFVER+V ++++ N +V VV ++ D F L+ ++ K + ++ + G + G AL+ ++ + S R E VP++++L++
Sbjct: 2 FPSLSAEYSTPRKDVVFLLDGSDGTR-SSFPAMRDFVQRVVEKFNIEANRDRVSVVQYSRDAEVHFYLNSYTKKEDILDRVTGLRHKGGRPLYTGAALQYVRDNVFTASSGSRRLEGVPQILILLSGGRS----FDSVDAAASSLKELGVLTLGIGSRGSDSRELQRISYEANYALSVADFSELPNVQEQLLASVQ-------TVAMSAGPLLTTYIVNEVVIKDVVFLLDGS-DGTRNTFPAMRAFVQRLVEQLNVEANRDRISVVQYSRDAEVNF-----YLNTYTAKADVLNTVRGLRHRGGRPLNTGAALQYVRDSVFTASAGSRKQEGIPQVLILLSGGRSNDN-VDTPASALKESGVLILGIG-TRNSSREVQRITNDPAYAQSLNDFSDLPGVQQQFYSSLNNVLVQVKPMTPTVAGKTEFPRMARRDVVFLLDGSDGTRSSFSAMR-DFVERMVERLNVSENRDRVSVVQYSRDPEAHFYLNTYSRKEDALDTIRGLRHKGGRPLNTGAALQYLRDNVFTASSGSRRVEGVPQLLILLS 527 HSP 3 Score: 73.559 bits (179), Expect = 2.094e-12 Identity = 115/541 (21.26%), Postives = 228/541 (42.14%), Query Frame = 3
Query: 2673 QDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNT-----AIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISE-KELNDIA-SSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTI-----------CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTS--------TNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYI---------SSELCDVIKGTNKECSTKQLDLMFALDSSSS---IGPDYWQTEL-DFVERIVRDMDINANFT-----KVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGV 4160
+D + + D+I ID S S+G DF+ ++V LD+R D+ R+ + + ++S+ + I ++ + G AI + I N + LVVLT G + + +S + L G ++ +G+G + ++ ++L IA SS + V +F +L K I+ + I +P I KAA D++F++ S N FK++ +F+ +L L +++ ++ + + + D + Y N + I +T + G I + V K + +++++ G + R+++ A LK G++V+ +G+ E +L ++ S + F L N I LC +K +EC +LD++ D SS +T++ ++RI++ I+ N +VG++ ++ A + LD FT KY + + + + G + A + + S + KV++ +TDG+
Sbjct: 1010 RDPLNLGRKDIIFLIDGSDSVGQSGVAHIRDFILKVVDQLDVRPDQVRVALVQYGERPKTEFSLNSHDNKQSVISAIKRLRHMGGRGADLAEAIKYVIRNELQASAGVRLAQASQHLVVLTGGRS----TSDVSTYGSILKGSRVNCIGIGAENADSRQLIQIATSSDDVLQVPSFPNLPNIQNKFIARLSGSIVVEPPIEIDETTPGLPQAKAA-DIVFLVDGSINLGRNNFKEVMEFILNLIDLFYTERDRLQIGLAHYAT-DVTDVFYLNTYNNKDDIINAITRAEYKGGREIRTGSAIRHVQKTHFVKEKGSRKDEGIPQILMVVTGGRS-RDDSKSAALGLKASGVRVYAVGV--GDIEDELNNLGSEATTVARASTFQELSELNEQILDTLDQDVKGINLCTGVKEATREC---KLDVLVGFDVSSQNIFAAQRSLETKVAAILQRIIQMQSISCNSGQAPSIQVGMLAMDS-ASEPVQLD-FTNKYTDLLEPFKALRNRGPFVLNGATIKAYADRFKSRPSDTIKVVIHLTDGL 1536 HSP 4 Score: 52.373 bits (124), Expect = 5.567e-6 Identity = 35/126 (27.78%), Postives = 64/126 (50.79%), Query Frame = 3
Query: 138 GKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRAL---NSTLYDIVPEMRPTSEIDRILLILSDGSNTDELS 506
G RD+ ++D +S I N EF + + + + + +N RVS + Y D K EFL +H++ + A+R R GG+ G+AL + T+Y R + + L++++ G + D++S
Sbjct: 815 GDKRDVVFLIDGTSKIRN-EFPAIRDMVQRVVEKLDVGLDNVRVSVVQYSDDPKLEFLLNEHSTKEEVRQAIRRLRNKGGNELNTGKALEYVSKTIYQRSAGSRVEEGVPQFLILVTGGKSNDDVS 939
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: col6a3 (collagen, type VI, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070501-8]) HSP 1 Score: 140.584 bits (353), Expect = 9.125e-33 Identity = 257/1132 (22.70%), Postives = 477/1132 (42.14%), Query Frame = 3
Query: 1065 FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEG-ATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETY-YPIEDMSKM--------ALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY-------DDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDK-ADREELKILSTFPNPFLR-QDFXXXX-------XXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNT--ALNRAR-QVFKIDY----RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQD-------ECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNE----ACQIPQKPTIC----KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNR---GNYIDSGFKEDYKNFESSIEDMTTSTNGV--------FIGKALTSAR-VLFKSDT---RKNATKTVIIIIDGEITDRNETFEEANL----LKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYI-------SSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALRECKNELL----NSTRKE-VPKVIVLVTDGVS 4163
F +++ L + ++ V+ YS E + L + K EI V L G + AL+ + S+ EG + ++L++ G + A LK++G+ IG S + +L ++S +Y + D S++ A T I + + D+ ++D S + + F +R +E+F + N+ R S+V ++ +A V H Y +DIL +V+ L+H GG AL + + S S+RL ++LL+ G S + V AA L+E GV T+G+G + +D EL+ +S N L DF E + ++ T+P + D++F++D S D T + +R+ + ++ N+ RIS++ YS + F L+ K D+ ++ LR G G LNT AL R VF + V ++++L + + D+ + A LK GV I+ +G ++NS + + + + + ++Q + + + Q + E M + DV+ +D S +F DFV R+V+ L++ ++R R+ + ++ + + +++ D I + G G A+ D +FT E VP++L++L+ GA ++ A K G+ I ++G + +EL I+ + +V FTDL + +N +P T + D++F++ S + T F M DF++ + + V + R+++++++R N+ Y N ++ E++ + G+ + G+AL R +F + + R +++++ G +F+ N LK+ G+ F IG G S + P AL V +FS L + S + E ST + D++F LD S + DFV+R+V +I AN +V VV ++ DA F L+ +T+K ++ V + G G AL+ ++ + S R E VP++++L++ G S
Sbjct: 4 FVQRMVEKLDVAENRDRISVVQYSREPEANFYLNTYTTKEEIVDAVRGLRHKGGRPLYTGEALQYVRDNVFTASSGSRRLEGVPQILVLLSGGRSFDSV---DAAASSLKELGVLTFGIGSRGSDSR-ELQRISYEPSYALSVSDFSELPNVQEQLLASVQVTSIPVTPTSPTVTAEYSTPRKDVVFLLDGSDGTR-SSFPAMRDFVQRVVEKFNIEANRDRVSVVQYSRDAEV-----HFYLNSYTKKEDILDRVKGLRHKGGRPLYTGAALQYVRDNVFTASSGSRRLEGVPQILILLSGGRSFDSVDA--AASSLKELGV--LTLGIGSRGSDSRELQRISYEANYALSVADFSELPNVQEQLLASVQTVAMSGTPTSPTLTADDTIPRKDVVFLLDGS-DGTRNTFPAMRAFVQRLVEQFNVEANRDRISVVQYSRDAEVNFYLNTYTAKADVLNTVRGLRHRG-GRPLNTGAALQYVRDSVFTASAGSRKQEGVPQVLILLSGGRSNDNVDTPASA--LKESGVLILGIG-TRNSSREVQRITNDPAYAQSLNDFSDLPGVQQQFYSSLNNVLVQVKPMTPTVAAEPRMARRDVVFLLDGSDGTR-SSFSAMRDFVERMVERLNVSENRDRVSVVQYSRDPEAHFYLNTYSRKEDALDTIRGLRHKGGRPLNTGAALQYLRDNVFTASSGSRRVEGVPQLLILLS-GARSF-DNVDTPASSLKELGVLIFAIG-SRSSDSQELQRISQEPSYALSVSDFTDLPSVQQQLFTNINKVFVAGVPTTSTTIAEGRRQRRDVVFLLDGS-DGTRNGFPAMKDFVQRMVEKLDVAENRDRISVVQYSREPGANF--------YLNTYTTKEEIVDAVRGLRHKGGRPLYTGEALQYVRDNVFTASSGSRRLEGVPQILVLLSG-----GRSFDSVNAAASSLKELGVLTFGIGSRGSDSRELQRISYEPSYALSVSDFSELPNVQEQLLASVQVTSIPVTPTSPTVTAEYSTPRKDVVFLLDGSDGTRSSFPAMR-DFVQRVVEKFNIEANRDRVSVVQYSRDAEVHFYLNSYTKKEDILDRVTGLRHKGGRPLYTGAALQYVRDNVFTASSGSRRLEGVPQILILLSGGRS 1097 HSP 2 Score: 124.405 bits (311), Expect = 9.135e-28 Identity = 267/1234 (21.64%), Postives = 511/1234 (41.41%), Query Frame = 3
Query: 993 QQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEG-ATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETY-YPIEDMSKM--------ALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY-------DDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDK-ADREELKILSTFPNPFLR-QDFXXXX-------XXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNT--ALNRAR-QVFKIDY----RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQD-------ECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGAN-DIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISN----------------EACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF----NRGNYIDSG-FKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTR-----KNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNL--------------------QRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALRECKNELLN---STRKE--VPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTF-YRILDFKTLKDYDTTFITRES 4355
Q+ D++F+LD S + F +++ L + ++ V+ YS + L + K EI V L G + AL+ + S+ EG + ++L++ G A LK++G+ IG S + +L ++S +Y + D S++ A T I + + D+ ++D S + + F +R +E+F + N+ R S+V ++ +A V H Y +DIL +V L+H GG AL + + S S+RL ++LL+ G S + V AA L+E GV T+G+G + +D EL+ +S N L DF E + ++ T+P + D++F++D S D T + +R+ + ++ LN+ RIS++ YS + F L+ K D+ ++ LR G G LNT AL R VF + + ++++L + + D+ + A LK GV I+ +G ++NS + + + + + ++Q + + + Q + E M + DV+ +D S +F DFV R+V+ L++ ++R R+ + ++ + + +++ D I + G G A+ D +FT E VP++L++L+ + D ++P A K G+ I ++G + +EL +++ + +V+ F+DL K +SN + P+K D+IF++ S + E F + +F++ + V + R+ ++++ N Y++S KE N + + + +G+A+ R + R + + V+++ G+ TD + A LK+ GI +IG ++ + P A VD+ L Q Y + + V +G + D++F +D +S I ++ D V+R+V +D+ + +V VV ++ D + EF L++ + K + A++R+ G + G+AL + +R E VP+ ++LVT G S +S + ++ + D+ +L+++S S Y I DF+ L + +T+ S
Sbjct: 779 QRRDVVFLLDGSDGTRNGFPAMKDFVQRMVEKLDVAENRDRISVVQYSREPGANFYLNTYTTKEEIVDAVRGLRHKGGRPLYTGEALQYVRDNVFTASSGSRRLEGVPQILVLLSGGRSFDSV---NAAASSLKELGVLTFGIGSRGSDSR-ELQRISYEPSYALSVSDFSELPNVQEQLLASVQVTSIPVTPTSPTVTAEYSTPRKDVVFLLDGSDGTR-SSFPAMRDFVQRVVEKFNIEANRDRVSVVQYSRDAEV-----HFYLNSYTKKEDILDRVTGLRHKGGRPLYTGAALQYVRDNVFTASSGSRRLEGVPQILILLSGGRSFDSVDA--AASSLKELGV--LTLGIGSRGSDSRELQRISYEANYALSVADFSELPNVQEQLLASVQTVAMSGTPTSPTLTADDTIPRKDVVFLLDGS-DGTRNTFPAMRAFVQRLVEQLNVEANRDRISVVQYSRDAEVNFYLNTYTAKADVLNTVRGLRHRG-GRPLNTGAALQYVRDSVFTASAGSRKQEGIPQVLILLSGGRSNDNVDTPASA--LKESGVLILGIG-TRNSSREVQRITNDPAYAQSLNDFSDLPGVQQQFYSSLNNVLVQVKPMTPTVAAEPRMARRDVVFLLDGSDGTR-SSFSAMRDFVERMVERLNVSENRDRVSVVQYSRDPEAHFYLNTYSRKEDALDTIRGLRHKGGRPLNTGAALQYLRDNVFTASSGSRRVEGVPQLLILLSGTRSFDNVDTP---ASSLKELGVLIFAIG-SRSSDSQELQRVSNEPSYALSVREFSDLSNVQQKLLSNINTALVEVTSLTTTVTVESEGPKK--------DIIFLIDGSEDVGRE-FPIIQEFVRRVVSNLNVGENKIRIGVVQYGDIPNADIYLNSHRTKEGVLNGIKELRQLRGTKRN--LGQAIDFVRREVLASGRGGRKEEGVPQFVVVVSGGKATD--NIRQSATALKQSGIFPLSIGTRDVGTQELQVTSYVPRFAYLVDDLPGLYTIQETLINTLTELSSEELAQLRPVYPTETVVPVPRGDKR-------DVVFLIDGTSKIRNEFPAIR-DMVQRVVEKLDVGLDNVRVSVVQYSDDPKLEFLLNEHSTKEEVRQAIRRLRNKGGNELNTGKALEYVSKTIYQRSAGSRVEEGVPQFLILVTGGKSNDDVSGPANQLKLSRVAPLAVGARDADA---EELRLISFSPELAYTIRDFQQLPGVEQQLLTKVS 1964 HSP 3 Score: 114.39 bits (285), Expect = 8.120e-25 Identity = 191/812 (23.52%), Postives = 359/812 (44.21%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFISKGR-----EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETY-YPIEDMSKMALFDTTDI-------AQDACQ----------IPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE----EEFTHIYDDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXXXXXXXXXCK------TTPAIKPQTCSSNG--DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRS-NGNGGDLNTALNRARQVFKIDYR-------ADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQ----DECT-----MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEV----YLNVPIDSNFENADMQKEINKIPFIE--GNTAI--GWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG-EFYNVKTFTDLEKFNAKTI 3212
D+IF++D S + + F V+ +L + ++GV+ Y DI + L S + K + + +L L G + A+ E ++ GR EG + V++V+ G T A LK+ GI + IG V +L S + Y ++D+ + T I +++ Q +P P+ K D+ ++D +S+++ +E+ +R +E+ ++G++ R S+V ++++ +E E T +++ + + L++ GG KAL S++ Y +S S+ + ++L+T G S + V A L+ S V +G D AD EEL+++S P + +DF++ + ++ + +TPA+ P+ + G DIIF++D S IR +L + L++ R++++ Y + TEF L+ K+ + AIK+LR G G DL A+ + V + + + A ++ +V+ T T+ D + ILKG V I +G + S +++ + + + + + + DE T K D++ +D S +LG NF+ ++F+ ++ +DR +IG+ + ++V YLN + N D IN I E G I G AI + F K + E +P++L+V+T G + + + +A+ K G+++ +VGVG E ELN++ S TF +L + N + +
Sbjct: 1581 DIIFLIDGSEDVGREFPIIQEFVRRVVSNLNVGENKIRIGVVQYGDIPNADIYLNSHRTKEGVLNGIKELRQLRGTKRNLGQAIDFVRREVLASGRGGRKEEGVPQFVVVVSGG---KATDNIRQSATALKQSGIFPLSIGT-RDVGTQELQVTSYVPRFAYLVDDLPGLYTIQETLINTLTELSSEELAQLRPVYPTETVVPVPR---GDKRDVVFLIDGTSKIR-NEFPAIRDMVQRVVEKLDVGLDNVRVSVVQYSDDPKLEFLLNEHSTK--EEVRQAIRRLRNKGGNELNTGKALEYVSKTIYQRSAGSRVEEGVPQFLILVTGGKSNDDVSG--PANQLKLSRVAPLAVGARD-ADAEELRLISFSPELAYTIRDFQQLPGVEQQLLTKVSTMTRDEISTPAV-PRDPLNLGRKDIIFLIDGSDSVGQSGVAHIRDFILKVVDQLDVRPDQVRVALVQYGERPKTEFSLNSHDNKQSVISAIKRLRHMGGRGADLAEAI---KYVIRNELQASAGVRLAQASQHLVVLTGGRSTS--DVSTYGSILKGSRVNCIGIGAENADSRQLIQIATSSDDVLQVPSFPNLPNIQNKFIARLSGSIVVEPPIEIDETTPGLPQAKAADIVFLVDGSINLGRNNFKEVMEFILNLIDLFYTERDRLQIGLAHYATDVTDVFYLNT-----YNNKD--DIINAITRAEYKGGREIRTGSAIRHVQKTHFVKEKGSRKDEGIPQILMVVTGGRSR--DDSKSAALGLKASGVRVYAVGVGD--IEDELNNLGSEATTVARASTFQELSELNEQIL 2362 HSP 4 Score: 88.5817 bits (218), Expect = 6.145e-17 Identity = 137/550 (24.91%), Postives = 246/550 (44.73%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTF----NSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKGR-----EDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTI-------SNEACQI-PQKPT---ICKAAGD---LIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTS--------TNGVFIGKALTS-ARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL----------DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALREC-KNELLNSTR---KEVPKVIVLVTDGVSTGGLS 4178
K D+I ID S +G E F I +FV R+V NL++ +++ RIG+ + N+++YLN S+ + I ++ + G +G AI + GR E VP+ +VV++ G A D + + SA K GI +S+G + + +EL + F Y V L I S E Q+ P PT + GD ++F++ ++ K F + D ++ + + V +N RV++++ Y D E N S+ E++ + N + GKAL ++ +++ S + + +I++ G+ D + AN LK + +G +E +L+ ++ SP+ A + +F L + L V T E ST + D++F +D S S+G DF+ ++V +D+ + +V +V + + EF L+ K ++ISA+KR+ G D+ EA++ +NEL S + + +V++T G ST +S
Sbjct: 1579 KKDIIFLIDGSEDVGRE-FPIIQEFVRRVVSNLNVGENKIRIGVVQYGDIPNADIYLN----SHRTKEGVLNGIKELRQLRGTKRNLGQAIDFVRREVLASGRGGRKEEGVPQFVVVVSGGKATD---NIRQSATALKQSGIFPLSIGT-RDVGTQELQVTSYVPRFAYLVDDLPGLYTIQETLINTLTELSSEELAQLRPVYPTETVVPVPRGDKRDVVFLIDGTS-KIRNEFPAIRDMVQRVVEKLDVGLDNVRVSVVQ-----YSDDPKLEFLLNEHSTKEEVRQAIRRLRNKGGNELNTGKALEYVSKTIYQRSAGSRVEEGVPQFLILVTGGKSND--DVSGPANQLKLSRVAPLAVGARDADAE-ELRLISFSPELAYTIRDFQQLPGVEQQL---LTKVSTMTRDEISTPAVPRDPLNLGRKDIIFLIDGSDSVGQSGVAHIRDFILKVVDQLDVRPDQVRVALVQYGERPKTEFSLNSHDNKQSVISAIKRLRHMGGRGADLAEAIKYVIRNELQASAGVRLAQASQHLVVLTGGRSTSDVS 2107 HSP 5 Score: 73.559 bits (179), Expect = 2.406e-12 Identity = 115/541 (21.26%), Postives = 228/541 (42.14%), Query Frame = 3
Query: 2673 QDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNT-----AIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISE-KELNDIA-SSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTI-----------CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTS--------TNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYI---------SSELCDVIKGTNKECSTKQLDLMFALDSSSS---IGPDYWQTEL-DFVERIVRDMDINANFT-----KVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGV 4160
+D + + D+I ID S S+G DF+ ++V LD+R D+ R+ + + ++S+ + I ++ + G AI + I N + LVVLT G + + +S + L G ++ +G+G + ++ ++L IA SS + V +F +L K I+ + I +P I KAA D++F++ S N FK++ +F+ +L L +++ ++ + + + D + Y N + I +T + G I + V K + +++++ G + R+++ A LK G++V+ +G+ E +L ++ S + F L N I LC +K +EC +LD++ D SS +T++ ++RI++ I+ N +VG++ ++ A + LD FT KY + + + + G + A + + S + KV++ +TDG+
Sbjct: 1978 RDPLNLGRKDIIFLIDGSDSVGQSGVAHIRDFILKVVDQLDVRPDQVRVALVQYGERPKTEFSLNSHDNKQSVISAIKRLRHMGGRGADLAEAIKYVIRNELQASAGVRLAQASQHLVVLTGGRS----TSDVSTYGSILKGSRVNCIGIGAENADSRQLIQIATSSDDVLQVPSFPNLPNIQNKFIARLSGSIVVEPPIEIDETTPGLPQAKAA-DIVFLVDGSINLGRNNFKEVMEFILNLIDLFYTERDRLQIGLAHYAT-DVTDVFYLNTYNNKDDIINAITRAEYKGGREIRTGSAIRHVQKTHFVKEKGSRKDEGIPQILMVVTGGRS-RDDSKSAALGLKASGVRVYAVGV--GDIEDELNNLGSEATTVARASTFQELSELNEQILDTLDQDVKGINLCTGVKEATREC---KLDVLVGFDVSSQNIFAAQRSLETKVAAILQRIIQMQSISCNSGQAPSIQVGMLAMDS-ASEPVQLD-FTNKYTDLLEPFKALRNRGPFVLNGATIKAYADRFKSRPSDTIKVVIHLTDGL 2504 HSP 6 Score: 64.6994 bits (156), Expect = 1.026e-9 Identity = 74/307 (24.10%), Postives = 139/307 (45.28%), Query Frame = 3
Query: 3330 DFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGV--------FIGKALTSAR-VLFKSDT---RKNATKTVIIIIDGEITDRNETFEE----ANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYI-------SSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALRECKNELL----NSTRKE-VPKVIVLVTDGVS 4163
DF++ + + V + R+++++++R ++ F Y N ++ E++ + G+ + G+AL R +F + + R +++++ G +F+ A+ LK+ G+ F IG G S + P AL V +FS L + S + E ST + D++F LD S + DFV+R+V +I AN +V VV ++ DA F L+ +T+K ++ VK + G G AL+ ++ + S R E VP++++L++ G S
Sbjct: 3 DFVQRMVEKLDVAENRDRISVVQYSR--EPEANF---YLNTYTTKEEIVDAVRGLRHKGGRPLYTGEALQYVRDNVFTASSGSRRLEGVPQILVLLSG-----GRSFDSVDAAASSLKELGVLTFGIGSRGSDSRELQRISYEPSYALSVSDFSELPNVQEQLLASVQVTSIPVTPTSPTVTAEYSTPRKDVVFLLDGSDGTRSSFPAMR-DFVQRVVEKFNIEANRDRVSVVQYSRDAEVHFYLNSYTKKEDILDRVKGLRHKGGRPLYTGAALQYVRDNVFTASSGSRRLEGVPQILILLSGGRS 298 HSP 7 Score: 52.373 bits (124), Expect = 7.060e-6 Identity = 35/126 (27.78%), Postives = 64/126 (50.79%), Query Frame = 3
Query: 138 GKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRAL---NSTLYDIVPEMRPTSEIDRILLILSDGSNTDELS 506
G RD+ ++D +S I N EF + + + + + +N RVS + Y D K EFL +H++ + A+R R GG+ G+AL + T+Y R + + L++++ G + D++S
Sbjct: 1783 GDKRDVVFLIDGTSKIRN-EFPAIRDMVQRVVEKLDVGLDNVRVSVVQYSDDPKLEFLLNEHSTKEEVRQAIRRLRNKGGNELNTGKALEYVSKTIYQRSAGSRVEEGVPQFLILVTGGKSNDDVS 1907 HSP 8 Score: 51.6026 bits (122), Expect = 9.883e-6 Identity = 79/332 (23.80%), Postives = 151/332 (45.48%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGE--EFI----SKGREGAKKTVILVTDGA--DNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYP----IEDMSKM--ALFDTTDIAQDA-----CQ-IPKPKRICSAKLDIALIVDCSSQ------VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAV------VEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSME 1898
D++F++D S +L + + + F N+I + Q+G+ Y+ + L + NK +I +++ E G + + R + F+ S+ EG + +++VT G D+S + A LK G+RV +G+G I+D+LN + S T +++S++ + DT D QD C + + R C KLD+ + D SSQ L V +L +R I+ + N + + A+ V+ +FT+ Y D+L+ ++L++ G F + + F + ++ ++V+ LT+GL +
Sbjct: 2189 DIVFLVDGSINLGRNNFKEVMEFILNLIDLFYTERDRLQIGLAHYATDVTDVFYLNTYNNKDDIINAITRAEYKGGREIRTGSAIRHVQKTHFVKEKGSRKDEGIPQILMVVTGGRSRDDSKSA-----ALGLKASGVRVYAVGVG--DIEDELNNLGSEATTVARASTFQELSELNEQILDTLD--QDVKGINLCTGVKEATREC--KLDVLVGFDVSSQNIFAAQRSLETKVAAIL--QRIIQMQSISCNSGQAPSIQVGMLAMDSASEPVQLDFTNKYTDLLEPFKALRNRGPFVLNGATIKAYADRFKSRPSDTIKVVIHLTDGLDAQ 2507
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Match: VIT (si:ch1073-291c23.1 [Source:ZFIN;Acc:ZDB-GENE-050208-114]) HSP 1 Score: 109.768 bits (273), Expect = 1.045e-24 Identity = 85/332 (25.60%), Postives = 155/332 (46.69%), Query Frame = 3
Query: 3219 EACQIP-QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKA---------LFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
A ++P Q CK D+ F+M S + RFK DF+ +++ V + I+++ + K+ + K+ + +I + +GKAL+ F SD R A ++++DG TDR E E + L ++ GI +F + + G V S DKA L + ++ L++ + +CD + + D+ F +D SSS+G ++T L FV + R+ +I+ T+VG+V + + R EF Q K +++A+KRI + G T G A+ ++L + ++ K+++++TDG S
Sbjct: 6 RAPEVPSQGDPNCKV--DMAFLMDGSWSIGKRRFKIQKDFLVEVSQALNVGVAGAMMGIIQYGDDPVTEFSLKQFSNSKDLKPAISKIVQKGGPSHVGKALSYINKQFFSDANGNRGGAPNVAVVLVDGWPTDRVE--EASRLARESGINIFFVTIEGPDDNEKQNVVESNFVDKAVCRTNGFFSLPIASWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGIVQYTYEQRLEFAFGQHNNKADLLNAIKRINYWSGGTSTGAAITYAADQLFSKSKPNKRKIMIVITDGRS 333 HSP 2 Score: 92.0485 bits (227), Expect = 5.926e-19 Identity = 96/391 (24.55%), Postives = 175/391 (44.76%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFV---GLSKNSPYNFYEN--------LAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE----FYNVKTFTDLEKFNAKTISNEACQIPQKP 3245
D+ F++D S + + ++ Q+LN+ + + II Y D+ TEF L + +D+ AI ++ G + AL+ + F D R + V+ D PT++ + S + + G+ I FV G N N E+ + S+ I + + K + + +CD + + + D+ ID S+S+G NF+ L FV + + ++ TR+GI + E L + AD+ I +I + G T+ G AI A D LF+K + + K+++V+TDG + D +P ++ + G+ S+G+ ++ EL IA+ + F+ V F +L K+ +K I N + +P
Sbjct: 21 DMAFLMDGSWSIGKRRFKIQKDFLVEVSQALNVGVAGAMMGIIQYGDDPVTEFSLKQFSNSKDLKPAISKIVQKGGPSHVGKALSYINKQFFSDANGNRGGAPNVAVVLVDGWPTDRVEEASR--LARESGINIFFVTIEGPDDNEKQNVVESNFVDKAVCRTNGFFSLPIASWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGIVQYTYEQRLEFAFGQHNNKADLLNAIKRINYWSGGTSTGAAITYAADQLFSKSKPNKRKIMIVITDGRSYDDVRAPALAVHRT---GVIAYSIGIAWA-AQDELEYIATDPDREHSFF-VDEFDNLYKYVSKIIHNICNEFNSQP 404 HSP 3 Score: 83.9593 bits (206), Expect = 3.042e-16 Identity = 88/388 (22.68%), Postives = 178/388 (45.88%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGV-------GQQISEKELND--IASSGEFYN--VKTFTDLEKFNAKTISNEACQIPQ--KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDK--ALFVDNFSNLQRYNAYISSELCD 3788
K+D+ +D S S+G F+I+ DF+ + Q L++ +GI + + + + D++ I+KI G + +G A+ F+ R P V VVL DG + + E+ ++ A+ GI I V + Q + E D + + F++ + ++ L K + + C + C A D+ FV+ S++ + F+ + F+ ++T+ + +TRV I+++ ++ F + + + ++I+ + + G G A+T A S ++ N K +I+I DG D + A + + G+ ++IG+ ++ +L+ +A+ PD+ + FVD F NL +Y + I +C+
Sbjct: 19 KVDMAFLMDGSWSIGKRRFKIQKDFLVEVSQALNVGVAGAMMGIIQYGDDPVTEFSLKQFSNSKDLKPAISKIVQKGGPSHVGKALSYINKQFFSDANGNRGGAPNVAVVLVDGWPTDRVEEASRL----ARESGINIFFVTIEGPDDNEKQNVVESNFVDKAVCRTNGFFSLPIASWFALRK-AVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGIVQYTYEQRLEFAFGQHNNKADLLNAIKRINYWSGGTSTGAAITYAADQLFSKSKPNKRKIMIVITDGRSYD--DVRAPALAVHRTGVIAYSIGIAW-AAQDELEYIATDPDREHSFFVDEFDNLYKYVSKIIHNICN 398 HSP 4 Score: 83.5741 bits (205), Expect = 3.741e-16 Identity = 89/382 (23.30%), Postives = 172/382 (45.03%), Query Frame = 3
Query: 942 TTSNYIEISTPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIA-----QDACQIPKPKRICSAKL-----------DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN 2018
TT+ E+ P Q N ++D+ F++D S S+ + + F V ++L + + + +G++ Y D L N +++ +SK+ G + + AL ++F S R GA +++ DG PT + E + ++ GI + + + D K + E+ + + + + F + IA + A Q P KR+C DIA ++D SS V + L F +FE+ TR +V + E +E F + D+L ++ + + G T A+ A+ + KSK N +++++++T+G S + V+ A + +GV +IG+ A ++EL+ ++T P+
Sbjct: 3 TTARAPEV--PSQGDPNCKVDMAFLMDGSWSIGKRRFKIQKDFLVEVSQALNVGVAGAMMGIIQYGDDPVTEFSLKQFSNSKDLKPAISKIVQKGGPSHVGKALSYINKQFFSDANGNRGGAPNVAVVLVDGW---PTDRVEEASRLARESGINIFFVTIEGP---DDNEKQNVVESNFVDKAVCRTNGFFSLPIASWFALRKAVQ-PLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGIVQYTYEQRLEFAFGQHNNKADLLNAIKRINYWSGGTSTGAAITYAADQLFSKSKPNKRKIMIVITDGRSYDDVRA--PALAVHRTGVIAYSIGIA-WAAQDELEYIATDPD 372 HSP 5 Score: 56.6102 bits (135), Expect = 1.649e-7 Identity = 41/153 (26.80%), Postives = 74/153 (48.37%), Query Frame = 3
Query: 114 LALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTS-SPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
L + C+ DIA ++D SSS+ G F++ L + F I +TRV + Y + EF F +H + + LN R++ GG S+ G A+ + + +P +I+++++DG + D++ LA + GV Y++ I
Sbjct: 212 LVCSKTCL-NANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGIVQYTYEQRLEFAFGQHNNKADLLNAIKRINYWSGGTST--GAAITYAADQLFSKSKPNKR--KIMIVITDGRSYDDVRAPALA--VHRTGVIAYSIGIA 357
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: ENSXETT00000014204.1 (collagen type VI alpha 5 chain [Source:NCBI gene;Acc:100486063]) HSP 1 Score: 249.595 bits (636), Expect = 6.312e-66 Identity = 275/1128 (24.38%), Postives = 522/1128 (46.28%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNAL-----RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQ--DFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNT--ALNRARQVF-------KIDYRADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXX-XXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-------DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK---GR--EDVPKVLVVLTDGAN-DIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQK----PTICKAA---------GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED--YKNFESSIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST--RKEVPKVIVLVTDG 4157
D++F++D S S+ + F ++ + + Q+G++ YSD L S NK ++ K++ L+ G T +L + E S+ EG + +++TDG +P + +K GI + IG+ +V+ +LN+++S + Y + D + + T + + A Q + ++C + + DI L+V+ ++++ + F + ++GINK R LV++ N+ E + Y +IL+ ++++K+V G+T +AL + +++ ++ S+ ++++++T G S + + + A +L+ G+ + +G K DR+ + S F Q DF + +++ E+ +K C + D++F+VD ST + ++ ++ + LN+ RI + YS TEF L+ TK +I IK+ + GG L T AL R F +I+Y + +VV+ + K +D++ AE LK GV I VG+ NS E +A+ + + ++ +I + Q + + D++ ID+S+S+GP NFQ+ F++++V LD+ R+G+ ++ E L + +++ E ++ I K+P+ G G A+ +FTK GR + V ++ VV+T+G + D P A K + G+++ +VG Q I++ EL+ IAS NV++F L + +I K P I +A D+ F++ S + E F+ M FM L + V R +++++ + E K + +I + G G+ALTS + LF D +++++I DGE DR E A ++ DGI +F IG+ E S +K FV+NF +L+ ++ ELC T + C + D++F +DSS+SI D ++T +F+E +V+ +I + ++G++ F+++ ++EF L+++ K + SA++ I T +GEAL+ S R + ++++TDG
Sbjct: 34 DIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAI---AVKNAGIILYAIGIKDAVLS-ELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTY-NDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTL--TEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTY-RGGPLKTGHALEFVRSTFFIEEAGSRINY-GNPQFLVVITSSK----SEDAVRRHAEELKSVGVTTISVGIG-NSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGF-QNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDR--VTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELC-----TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDG 1136 HSP 2 Score: 240.35 bits (612), Expect = 4.548e-63 Identity = 274/1115 (24.57%), Postives = 504/1115 (45.20%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALR--RSG---EEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF--DTTD--IAQDACQ------IPKPKRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLR----QDFXXXXXXXXXXXXXXCK-------TTPAIK---PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF--NRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEIT--DRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKEVPKVIVLVTDGVS 4163
D++F++D+S+S+ + Q++ F ++I L + I ++G+ YS + + L + K EI K + + G +AL RS EE S+ G + ++++T + AE+LK +G+ I +G+G S K +L K+++ + + ++ D + IA+D Q +P +CS+ DI ++D SS + + +F + + ++ ++ R LV +++E +E F Y+ IL + L + GG AL+ + + K + ++ V++TNG SM+ + A L+ SGV++ +G + D EL I+++ P P + F + +L + K C PAI + C + DI F++D S E ++ ++ M+ + + R ++ YSD TEF + E T++ + AI Q+ G G AL +Q+F D V + +V+ TD +D ++EA ++ DG+ I +G+ + S + + N + + K + +C + C K D++ +D S S+ ++++ +F+ +V+ ++ DR +IG+ F+SE P++ ++Q I I + T +G A+ Y GR + + L+V+TDG A D +P A + G+ I ++GV QQ + +L +IA E Y +F L N K I E C PQ+ D+IF++ +SA+ T F+ M F++++ ++V K+N + + + N + ++ + + + KAL R+ F + R + +I++ DG T DR + LK DGI VF +G+ GK +L+ +A PD+ V N+ L + I+ +CD K CS +QLDL+F +D S+SI + + F++ IV I+ N ++GV ++ + +KEF L+++ M + I T G+ LR K + N RK VP+ ++++TDG+S
Sbjct: 432 DVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRR---HAEELKSVGVTTISVGIGNSDRK-ELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPA---VCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYE-ILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNF--TKPAAKLRRSGVEVFAVGFQNIND-TELDIIASHP-PRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITD---GESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTP-----EACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGV-QQANNTQLLEIAGKQEQVYYEDSFDSLAFLN-KNIMFEICN-PQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGV-GKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESK---PACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMS 1516 HSP 3 Score: 214.927 bits (546), Expect = 2.677e-55 Identity = 267/1143 (23.36%), Postives = 515/1143 (45.06%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFD-----TTDIAQDAC-QIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHI--YDDILKKV-ESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPF----------LRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN--GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVG-----------LSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF------KQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--------NFESSIEDMTTSTNGVFIGKALTSARVLFKSDT--RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVSTGGLSPEL 4187
Q D++ ++++++ + +++ F +++ +L + I ++G++ Y+D L S +KTEI + + ++ +EG T+ AL + S+ E + +I+VT+G + +P A++LK GI V V+G + + QL + SS + +Y ++D FD T + ++ C I ++ S + D+ +VD S+ + + F I Q +GINK R L ++ E H ++ILK + E+ + GG + AL +F+++ S+ + +V++T+ S + V+ + A +L+ GV ++G+G+ +DR+EL+ ++T P F L+QD + PA+ CSS DI+F++D+S+ + R + + +L+IS + R+ ++ YSDE E +L+ K +I I +L G AL+ R+ V +I V+ T+ D+ ++ A L+ GV++ VG ++ + P N+ S + + K ++ +F + ++ C ++ D+ ID S S+ PE+F+ F+ ++ + +R R G+ ++ I + ++ I++I + G T G A+ + LF +D VP+ LVV+TDG + + +A + + GI I ++GV + E+E+ DIA S E + V F L K I N+ + P CK D++F++ SSA+ S+ ++ M +FM+S+ K + + ++ +++F+ S KE++ +S+I + + G +G+AL F + R N + +I+I DGE D A ++ G+ ++ IG+ + L+ ++ + D+F +L N I E+C+ + K T+ D++F LD+S+SI ++ FVE +V D + + + G V + T+ ++F L+ ++ K ++ AV + G T +AL + S R + +++LVTDG +T P L
Sbjct: 235 QADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVGTNIKYDR-QLQEASSKPDEKFFYQLDD------FDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVR--RHAEELKSVGVTTISVGIGN-SDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAV----CSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTN---GQSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNV----ESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEAL-TSMKQLFVNAAKDRPHKVPQSLVVITDGESQ--DRVTEAAAEIRNDGITIFAIGVKNAV-EEEIRDIAGSNEKMFFVNNFDSL-----KVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFS------SETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQD--AVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKK---TEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNL 1333 HSP 4 Score: 193.356 bits (490), Expect = 1.093e-48 Identity = 228/1000 (22.80%), Postives = 445/1000 (44.50%), Query Frame = 3
Query: 1002 DLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK---GR--EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNK--MSSTETYYPIEDMS-KMALFDTTDIAQDACQIPKPKR------ICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---THIYDDILK-KVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLV----VLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSL-SEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWA-----IGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKL--GGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--NFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGT--NKECSTKQLDLMFALDSS 3857
D++F++D SSS+ + + F + V+ +L I + +VG+++YSD + L L + K EI F++KL G AL ++ +K GR +G ++ +++T+G KP A KL++ G+ V +G L +I + +++ E++ + ++ ++ +I + +P R + + + DI ++D S + ++ + F I F++G N+ RF +V +++ V EF H +LK + ++ +GG T +AL + F +K+ V V++T+G S +RV +AA +++ G+ I IG+ + + E I + F +F K + + C P+ C + DI+F+VD S ++ ++ ++ M + ++ I +I +I +S ET EF L+ K +I AI+ ++ G + AL F R + + +++ TD +D++ + A+ ++ GV I +G+ + + E ++ ++ + + + I IC+ Q+ C ++ D+I +D S S+ F++ FV +V + + KD + G + + +++ D+ K + +P + G T A I T Y GR + +L+++TDGA + P + V L GI + +VGVG+ + + EL IA + ++ V+ + L+ + I+ C KP DL+F++ SA+ TS F FMK + + + R+ + +++ + E Y + + I+ ++ + GK L + F + RKN + +I++ DG D E+A L+ G+K+F+IG+ + S + SP V+ F L I +++C+ KECS LD+ ++D S
Sbjct: 633 DIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSD--VRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRV--TEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCT------PEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITD---GEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNP----QESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSY---GGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQ-ELQQIAGYPDRWFLVQNYKGLDNIH-DNITQVVCD-ESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSND--SVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECS---LDITISVDYS 1601 HSP 5 Score: 156.762 bits (395), Expect = 1.688e-37 Identity = 169/779 (21.69%), Postives = 357/779 (45.83%), Query Frame = 3
Query: 978 QNC-NNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKK---TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT-HIY---DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK----NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPT--NQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK 3257
+ C + ++ D+ F++D S S+ ++ F +I Q+ + GV+ YSD+ + + ++ +S++E L G T AL + F++ ++ K +++++TDG + A +++ GI + IG+ +V ++ + S E + + + + + D+A++ C P+ + K DI +VD S+ + ++ F + ++Q E+G ++ + L+ F++E +EEF + Y D+I + ++ + T + +AL + ++ SK N+K+ ++++T+G + + V A +++ GV I IG+ + + L+I + F L + + C + K + DIIF++D S T + ++ + + + + + + Y +F L+ TK DI A+ L AL R F Y R ++ I++L TD T ++ + ++ LK DG+ + VG+ K P + ++N +D + I ++CD + + C+ ++LD++ ID S S+ NF F+ IV + + ++R RIG+ +++ ++ + ++DM+K+I+ I ++ T G + + GR++VP+ L+V+TDG ++ + +A+++ G+KI S+G+G + S + + S Y V+TF L+ + ++ + C+ +P I K
Sbjct: 831 EGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTE---AAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVI-KNDLARELC---TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSE--TKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKY-TLPYFSASKGGRVNTKQYLIVITDGEAQDAVGN--PAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVA---DIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCD---ESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSS--GVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVA-QVCEPSDQPIIGK 1588 HSP 6 Score: 136.346 bits (342), Expect = 2.798e-31 Identity = 168/730 (23.01%), Postives = 337/730 (46.16%), Query Frame = 3
Query: 2112 TCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN-RARQVFK--IDYRA--DVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMI---CDAFKQRQDE-------C-TMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQIPQKPTI-CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF----VDNFSNLQR--YNAYISSELCDV--IKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
T +S DI+F++D S E + +++ + T + ++ +I +I YSD TEF L+ K D+ I+ L+ G G +L Q F RA V +I V+ TD +DS+ E I +K G+ + +G+ K++ + +AS + ++ + + +NMI C ++ + C T + D+++ ++ +T +G F+ +F+ +V NLD+ ++ RIG+ T+N E ++S ++ + I + ++EG T G A+ FT+ E V ++L+++T+G D ++ A + K GI + VG + +++L + +S + FY + F D E + + N I + K D++F++ SS + + F++M DF+ + V R+ + +++ + ++ + ++ + + E T + G AL R F ++ +R N +++ + A LK G+ ++G+ G + +L+ +A+ D +F + + SNLQ+ N I+ ++ + CS+ + D++F +D SSSIGP +Q F+ ++V +DI+ + +VG+V ++ + R E L+ F EKY ++ + ++ + G G AL + ++ N R V ++ V++T+G S
Sbjct: 28 TEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITD---GQAQDSIREPAIAVKNAGIILYAIGI-KDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEG--DSSDTLTEPAKELKSRGISVYVVGTNIKY-DRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGI-GNSDRKELEKIAT-DPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQS 748 HSP 7 Score: 122.094 bits (305), Expect = 5.697e-27 Identity = 115/497 (23.14%), Postives = 226/497 (45.47%), Query Frame = 3
Query: 2682 CTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAI-----GNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFN-------AKTISNEACQIPQKPTICKAA--GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF--VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKR-IIFDDGATDIGEAL 4082
CT + D++ +D S S+G ENF +F+ +V D+ D+ +IG+ ++ ++S D+ K I + + G T G ++ + ++ ++ E VP++ VV+TDG A D P A+ K GI + ++G+ + ELN+IAS + Y+V F L+ + T+ A Q Q +C+ A D++ ++ S+ F++ +F+ L V R+ ++ +N ++ + + + E SI++M + G+AL + S ++ + +II+ +G+ +D E A LK GI V+ +G K ++ + PD+ F +D+F + + + LC I + S + D++F +DSS+S+G ++Q DF+ I+ +++ N ++G+ ++ + EF L+ + K ++ +K + G G AL
Sbjct: 27 CTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREP---AIAVKNAGIILYAIGIKDAVL-SELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILE-SIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSD--TLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHAL 516 HSP 8 Score: 59.6918 bits (143), Expect = 5.663e-8 Identity = 53/215 (24.65%), Postives = 101/215 (46.98%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEF--ISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQI---PKPKRICSAKLDIALIVDCSSQVQL 1610
C+++QLDL+F++D S+S+ + + F ++ S I ++GV YS + L + ++++K + + L+ T+ LR + F + GR+ + +I++TDG N + +A L+ G+++ IG+GL + + S + Y +E + +AQ C+ P + CS LDI + VD S + L
Sbjct: 1398 CSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNE---DAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQ-VCEPSDQPIIGKECS--LDITISVDYSRRAML 1606 HSP 9 Score: 57.7658 bits (138), Expect = 1.763e-7 Identity = 40/153 (26.14%), Postives = 79/153 (51.63%), Query Frame = 3
Query: 117 ALDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNST--LYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
A+ + C+ + DI ++D S SI +F+ + + +S F + N R + Y D +TEF ++H + L A+ ++GG ++ G AL S L+ + RP ++ + L++++DG + D V + A +I+ G+TI+ + +
Sbjct: 828 AVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGG-GTLTGEALTSMKQLFVNAAKDRP-HKVPQSLVVITDGESQDR--VTEAAAEIRNDGITIFAIGV 976
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: ENSXETT00000014315.1 (collagen type VI alpha 5 chain [Source:NCBI gene;Acc:100486063]) HSP 1 Score: 248.825 bits (634), Expect = 8.934e-66 Identity = 275/1128 (24.38%), Postives = 522/1128 (46.28%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNAL-----RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQ--DFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNT--ALNRARQVF-------KIDYRADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXX-XXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-------DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK---GR--EDVPKVLVVLTDGAN-DIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQK----PTICKAA---------GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED--YKNFESSIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST--RKEVPKVIVLVTDG 4157
D++F++D S S+ + F ++ + + Q+G++ YSD L S NK ++ K++ L+ G T +L + E S+ EG + +++TDG +P + +K GI + IG+ +V+ +LN+++S + Y + D + + T + + A Q + ++C + + DI L+V+ ++++ + F + ++GINK R LV++ N+ E + Y +IL+ ++++K+V G+T +AL + +++ ++ S+ ++++++T G S + + + A +L+ G+ + +G K DR+ + S F Q DF + +++ E+ +K C + D++F+VD ST + ++ ++ + LN+ RI + YS TEF L+ TK +I IK+ + GG L T AL R F +I+Y + +VV+ + K +D++ AE LK GV I VG+ NS E +A+ + + ++ +I + Q + + D++ ID+S+S+GP NFQ+ F++++V LD+ R+G+ ++ E L + +++ E ++ I K+P+ G G A+ +FTK GR + V ++ VV+T+G + D P A K + G+++ +VG Q I++ EL+ IAS NV++F L + +I K P I +A D+ F++ S + E F+ M FM L + V R +++++ + E K + +I + G G+ALTS + LF D +++++I DGE DR E A ++ DGI +F IG+ E S +K FV+NF +L+ ++ ELC T + C + D++F +DSS+SI D ++T +F+E +V+ +I + ++G++ F+++ ++EF L+++ K + SA++ I T +GEAL+ S R + ++++TDG
Sbjct: 34 DIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAI---AVKNAGIILYAIGIKDAVLS-ELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTY-NDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTL--TEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTY-RGGPLKTGHALEFVRSTFFIEEAGSRINY-GNPQFLVVITSSK----SEDAVRRHAEELKSVGVTTISVGIG-NSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGF-QNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDR--VTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELC-----TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDG 1136 HSP 2 Score: 239.58 bits (610), Expect = 6.272e-63 Identity = 274/1115 (24.57%), Postives = 504/1115 (45.20%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALR--RSG---EEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF--DTTD--IAQDACQ------IPKPKRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLR----QDFXXXXXXXXXXXXXXCK-------TTPAIK---PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF--NRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEIT--DRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKEVPKVIVLVTDGVS 4163
D++F++D+S+S+ + Q++ F ++I L + I ++G+ YS + + L + K EI K + + G +AL RS EE S+ G + ++++T + AE+LK +G+ I +G+G S K +L K+++ + + ++ D + IA+D Q +P +CS+ DI ++D SS + + +F + + ++ ++ R LV +++E +E F Y+ IL + L + GG AL+ + + K + ++ V++TNG SM+ + A L+ SGV++ +G + D EL I+++ P P + F + +L + K C PAI + C + DI F++D S E ++ ++ M+ + + R ++ YSD TEF + E T++ + AI Q+ G G AL +Q+F D V + +V+ TD +D ++EA ++ DG+ I +G+ + S + + N + + K + +C + C K D++ +D S S+ ++++ +F+ +V+ ++ DR +IG+ F+SE P++ ++Q I I + T +G A+ Y GR + + L+V+TDG A D +P A + G+ I ++GV QQ + +L +IA E Y +F L N K I E C PQ+ D+IF++ +SA+ T F+ M F++++ ++V K+N + + + N + ++ + + + KAL R+ F + R + +I++ DG T DR + LK DGI VF +G+ GK +L+ +A PD+ V N+ L + I+ +CD K CS +QLDL+F +D S+SI + + F++ IV I+ N ++GV ++ + +KEF L+++ M + I T G+ LR K + N RK VP+ ++++TDG+S
Sbjct: 432 DVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRR---HAEELKSVGVTTISVGIGNSDRK-ELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPA---VCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYE-ILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNF--TKPAAKLRRSGVEVFAVGFQNIND-TELDIIASHP-PRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITD---GESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTP-----EACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGV-QQANNTQLLEIAGKQEQVYYEDSFDSLAFLN-KNIMFEICN-PQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGV-GKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESK---PACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMS 1516 HSP 3 Score: 214.542 bits (545), Expect = 3.913e-55 Identity = 266/1142 (23.29%), Postives = 513/1142 (44.92%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFD-----TTDIAQDAC-QIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHI--YDDILKKV-ESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPF----------LRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN--GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVG-----------LSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF------KQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--------NFESSIEDMTTSTNGVFIGKALTSARVLFKSDT--RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVSTGGLSPEL 4187
Q D++ ++++++ + +++ F +++ +L + I ++G++ Y+D L S +KTEI + + ++ +EG T+ AL + S+ E + +I+VT+G + +P A++LK GI V V+G + + QL + SS + +Y ++D FD T + ++ C I ++ S + D+ +VD S+ + + F I Q +GINK R L ++ E H ++ILK + E+ + GG + AL +F+++ S+ + +V++T+ S + V+ + A +L+ GV ++G+G+ +DR+EL+ ++T P F L+QD + PA+ CSS DI+F++D+S+ + R + + +L+IS + R+ ++ YSDE E +L+ K +I I +L G AL+ R+ V +I V+ T+ + + A L+ GV++ VG ++ + P N+ S + + K ++ +F + ++ C ++ D+ ID S S+ PE+F+ F+ ++ + +R R G+ ++ I + ++ I++I + G T G A+ + LF +D VP+ LVV+TDG + + +A + + GI I ++GV + E+E+ DIA S E + V F L K I N+ + P CK D++F++ SSA+ S+ ++ M +FM+S+ K + + ++ +++F+ S KE++ +S+I + + G +G+AL F + R N + +I+I DGE D A ++ G+ ++ IG+ + L+ ++ + D+F +L N I E+C+ + K T+ D++F LD+S+SI ++ FVE +V D + + + G V + T+ ++F L+ ++ K ++ AV + G T +AL + S R + +++LVTDG +T P L
Sbjct: 235 QADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVGTNIKYDR-QLQEASSKPDEKFFYQLDD------FDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVR--RHAEELKSVGVTTISVGIGN-SDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAV----CSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNG--QSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNV----ESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEAL-TSMKQLFVNAAKDRPHKVPQSLVVITDGESQ--DRVTEAAAEIRNDGITIFAIGVKNAV-EEEIRDIAGSNEKMFFVNNFDSL-----KVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFS------SETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQD--AVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKK---TEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNL 1333 HSP 4 Score: 192.971 bits (489), Expect = 1.370e-48 Identity = 228/1000 (22.80%), Postives = 444/1000 (44.40%), Query Frame = 3
Query: 1002 DLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK---GR--EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNK--MSSTETYYPIEDMS-KMALFDTTDIAQDACQIPKPKR------ICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---THIYDDILK-KVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLV----VLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWA-----IGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKL--GGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--NFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGT--NKECSTKQLDLMFALDSS 3857
D++F++D SSS+ + + F + V+ +L I + +VG+++YSD + L L + K EI F++KL G AL ++ +K GR +G ++ +++T+G KP A KL++ G+ V +G L +I + +++ E++ + ++ ++ +I + +P R + + + DI ++D S + ++ + F I F++G N+ RF +V +++ V EF H +LK + ++ +GG T +AL + F +K+ V V++T+G S +RV +AA +++ G+ I IG+ + + E I + F +F K + + C P+ C + DI+F+VD S ++ ++ ++ M + ++ I +I +I +S ET EF L+ K +I AI+ ++ G + AL F R + + +++ TD +D++ A+ ++ GV I +G+ + + E ++ ++ + + + I IC+ Q+ C ++ D+I +D S S+ F++ FV +V + + KD + G + + +++ D+ K + +P + G T A I T Y GR + +L+++TDGA + P + V L GI + +VGVG+ + + EL IA + ++ V+ + L+ + I+ C KP DL+F++ SA+ TS F FMK + + + R+ + +++ + E Y + + I+ ++ + GK L + F + RKN + +I++ DG D E+A L+ G+K+F+IG+ + S + SP V+ F L I +++C+ KECS LD+ ++D S
Sbjct: 633 DIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSD--VRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRV--TEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCT------PEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITD---GEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNP----QESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSY---GGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQ-ELQQIAGYPDRWFLVQNYKGLDNIH-DNITQVVCD-ESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSND--SVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECS---LDITISVDYS 1601 HSP 5 Score: 156.377 bits (394), Expect = 2.081e-37 Identity = 169/779 (21.69%), Postives = 357/779 (45.83%), Query Frame = 3
Query: 978 QNC-NNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKK---TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT-HIY---DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK----NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPT--NQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK 3257
+ C + ++ D+ F++D S S+ ++ F +I Q+ + GV+ YSD+ + + ++ +S++E L G T AL + F++ ++ K +++++TDG + A +++ GI + IG+ +V ++ + S E + + + + + D+A++ C P+ + K DI +VD S+ + ++ F + ++Q E+G ++ + L+ F++E +EEF + Y D+I + ++ + T + +AL + ++ SK N+K+ ++++T+G + + V A +++ GV I IG+ + + L+I + F L + + C + K + DIIF++D S T + ++ + + + + + + Y +F L+ TK DI A+ L AL R F Y R ++ I++L TD T ++ + ++ LK DG+ + VG+ K P + ++N +D + I ++CD + + C+ ++LD++ ID S S+ NF F+ IV + + ++R RIG+ +++ ++ + ++DM+K+I+ I ++ T G + + GR++VP+ L+V+TDG ++ + +A+++ G+KI S+G+G + S + + S Y V+TF L+ + ++ + C+ +P I K
Sbjct: 831 EGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTE---AAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVI-KNDLARELC---TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSE--TKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKY-TLPYFSASKGGRVNTKQYLIVITDGEAQDAVGN--PAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVA---DIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCD---ESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSS--GVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVA-QVCEPSDQPIIGK 1588 HSP 6 Score: 135.961 bits (341), Expect = 3.569e-31 Identity = 168/730 (23.01%), Postives = 337/730 (46.16%), Query Frame = 3
Query: 2112 TCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN-RARQVFK--IDYRA--DVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMI---CDAFKQRQDE-------C-TMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQIPQKPTI-CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF----VDNFSNLQR--YNAYISSELCDV--IKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
T +S DI+F++D S E + +++ + T + ++ +I +I YSD TEF L+ K D+ I+ L+ G G +L Q F RA V +I V+ TD +DS+ E I +K G+ + +G+ K++ + +AS + ++ + + +NMI C ++ + C T + D+++ ++ +T +G F+ +F+ +V NLD+ ++ RIG+ T+N E ++S ++ + I + ++EG T G A+ FT+ E V ++L+++T+G D ++ A + K GI + VG + +++L + +S + FY + F D E + + N I + K D++F++ SS + + F++M DF+ + V R+ + +++ + ++ + ++ + + E T + G AL R F ++ +R N +++ + A LK G+ ++G+ G + +L+ +A+ D +F + + SNLQ+ N I+ ++ + CS+ + D++F +D SSSIGP +Q F+ ++V +DI+ + +VG+V ++ + R E L+ F EKY ++ + ++ + G G AL + ++ N R V ++ V++T+G S
Sbjct: 28 TEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITD---GQAQDSIREPAIAVKNAGIILYAIGI-KDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEG--DSSDTLTEPAKELKSRGISVYVVGTNIKY-DRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGI-GNSDRKELEKIAT-DPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQS 748 HSP 7 Score: 121.709 bits (304), Expect = 6.622e-27 Identity = 115/497 (23.14%), Postives = 226/497 (45.47%), Query Frame = 3
Query: 2682 CTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAI-----GNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFN-------AKTISNEACQIPQKPTICKAA--GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF--VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKR-IIFDDGATDIGEAL 4082
CT + D++ +D S S+G ENF +F+ +V D+ D+ +IG+ ++ ++S D+ K I + + G T G ++ + ++ ++ E VP++ VV+TDG A D P A+ K GI + ++G+ + ELN+IAS + Y+V F L+ + T+ A Q Q +C+ A D++ ++ S+ F++ +F+ L V R+ ++ +N ++ + + + E SI++M + G+AL + S ++ + +II+ +G+ +D E A LK GI V+ +G K ++ + PD+ F +D+F + + + LC I + S + D++F +DSS+S+G ++Q DF+ I+ +++ N ++G+ ++ + EF L+ + K ++ +K + G G AL
Sbjct: 27 CTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREP---AIAVKNAGIILYAIGIKDAVL-SELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILE-SIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSD--TLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHAL 516 HSP 8 Score: 59.3066 bits (142), Expect = 6.105e-8 Identity = 53/215 (24.65%), Postives = 101/215 (46.98%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEF--ISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQI---PKPKRICSAKLDIALIVDCSSQVQL 1610
C+++QLDL+F++D S+S+ + + F ++ S I ++GV YS + L + ++++K + + L+ T+ LR + F + GR+ + +I++TDG N + +A L+ G+++ IG+GL + + S + Y +E + +AQ C+ P + CS LDI + VD S + L
Sbjct: 1398 CSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNE---DAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQ-VCEPSDQPIIGKECS--LDITISVDYSRRAML 1606 HSP 9 Score: 57.7658 bits (138), Expect = 1.917e-7 Identity = 40/153 (26.14%), Postives = 79/153 (51.63%), Query Frame = 3
Query: 117 ALDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNST--LYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
A+ + C+ + DI ++D S SI +F+ + + +S F + N R + Y D +TEF ++H + L A+ ++GG ++ G AL S L+ + RP ++ + L++++DG + D V + A +I+ G+TI+ + +
Sbjct: 828 AVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGG-GTLTGEALTSMKQLFVNAAKDRP-HKVPQSLVVITDGESQDR--VTEAAAEIRNDGITIFAIGV 976
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: ENSXETT00000014273.1 (collagen type VI alpha 5 chain [Source:NCBI gene;Acc:100486063]) HSP 1 Score: 248.054 bits (632), Expect = 1.519e-65 Identity = 275/1128 (24.38%), Postives = 522/1128 (46.28%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNAL-----RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQ--DFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNT--ALNRARQVF-------KIDYRADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXX-XXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-------DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK---GR--EDVPKVLVVLTDGAN-DIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQK----PTICKAA---------GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED--YKNFESSIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST--RKEVPKVIVLVTDG 4157
D++F++D S S+ + F ++ + + Q+G++ YSD L S NK ++ K++ L+ G T +L + E S+ EG + +++TDG +P + +K GI + IG+ +V+ +LN+++S + Y + D + + T + + A Q + ++C + + DI L+V+ ++++ + F + ++GINK R LV++ N+ E + Y +IL+ ++++K+V G+T +AL + +++ ++ S+ ++++++T G S + + + A +L+ G+ + +G K DR+ + S F Q DF + +++ E+ +K C + D++F+VD ST + ++ ++ + LN+ RI + YS TEF L+ TK +I IK+ + GG L T AL R F +I+Y + +VV+ + K +D++ AE LK GV I VG+ NS E +A+ + + ++ +I + Q + + D++ ID+S+S+GP NFQ+ F++++V LD+ R+G+ ++ E L + +++ E ++ I K+P+ G G A+ +FTK GR + V ++ VV+T+G + D P A K + G+++ +VG Q I++ EL+ IAS NV++F L + +I K P I +A D+ F++ S + E F+ M FM L + V R +++++ + E K + +I + G G+ALTS + LF D +++++I DGE DR E A ++ DGI +F IG+ E S +K FV+NF +L+ ++ ELC T + C + D++F +DSS+SI D ++T +F+E +V+ +I + ++G++ F+++ ++EF L+++ K + SA++ I T +GEAL+ S R + ++++TDG
Sbjct: 34 DIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREPAI---AVKNAGIILYAIGIKDAVLS-ELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTY-NDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTL--TEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTY-RGGPLKTGHALEFVRSTFFIEEAGSRINY-GNPQFLVVITSSK----SEDAVRRHAEELKSVGVTTISVGIG-NSDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGF-QNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDR--VTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELC-----TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDG 1136 HSP 2 Score: 238.81 bits (608), Expect = 1.130e-62 Identity = 274/1115 (24.57%), Postives = 504/1115 (45.20%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALR--RSG---EEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF--DTTD--IAQDACQ------IPKPKRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLR----QDFXXXXXXXXXXXXXXCK-------TTPAIK---PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF--NRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEIT--DRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKEVPKVIVLVTDGVS 4163
D++F++D+S+S+ + Q++ F ++I L + I ++G+ YS + + L + K EI K + + G +AL RS EE S+ G + ++++T + AE+LK +G+ I +G+G S K +L K+++ + + ++ D + IA+D Q +P +CS+ DI ++D SS + + +F + + ++ ++ R LV +++E +E F Y+ IL + L + GG AL+ + + K + ++ V++TNG SM+ + A L+ SGV++ +G + D EL I+++ P P + F + +L + K C PAI + C + DI F++D S E ++ ++ M+ + + R ++ YSD TEF + E T++ + AI Q+ G G AL +Q+F D V + +V+ TD +D ++EA ++ DG+ I +G+ + S + + N + + K + +C + C K D++ +D S S+ ++++ +F+ +V+ ++ DR +IG+ F+SE P++ ++Q I I + T +G A+ Y GR + + L+V+TDG A D +P A + G+ I ++GV QQ + +L +IA E Y +F L N K I E C PQ+ D+IF++ +SA+ T F+ M F++++ ++V K+N + + + N + ++ + + + KAL R+ F + R + +I++ DG T DR + LK DGI VF +G+ GK +L+ +A PD+ V N+ L + I+ +CD K CS +QLDL+F +D S+SI + + F++ IV I+ N ++GV ++ + +KEF L+++ M + I T G+ LR K + N RK VP+ ++++TDG+S
Sbjct: 432 DVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRR---HAEELKSVGVTTISVGIGNSDRK-ELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPA---VCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYE-ILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNF--TKPAAKLRRSGVEVFAVGFQNIND-TELDIIASHP-PRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITD---GESQDRVTEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCTP-----EACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQDAVGNP---AKAIRDHGVIIYAIGV-QQANNTQLLEIAGKQEQVYYEDSFDSLAFLN-KNIMFEICN-PQESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGV-GKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCDESK---PACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMS 1516 HSP 3 Score: 213.386 bits (542), Expect = 7.072e-55 Identity = 266/1142 (23.29%), Postives = 513/1142 (44.92%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFD-----TTDIAQDAC-QIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHI--YDDILKKV-ESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPF----------LRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN--GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVG-----------LSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF------KQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--------NFESSIEDMTTSTNGVFIGKALTSARVLFKSDT--RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVSTGGLSPEL 4187
Q D++ ++++++ + +++ F +++ +L + I ++G++ Y+D L S +KTEI + + ++ +EG T+ AL + S+ E + +I+VT+G + +P A++LK GI V V+G + + QL + SS + +Y ++D FD T + ++ C I ++ S + D+ +VD S+ + + F I Q +GINK R L ++ E H ++ILK + E+ + GG + AL +F+++ S+ + +V++T+ S + V+ + A +L+ GV ++G+G+ +DR+EL+ ++T P F L+QD + PA+ CSS DI+F++D+S+ + R + + +L+IS + R+ ++ YSDE E +L+ K +I I +L G AL+ R+ V +I V+ T+ + + A L+ GV++ VG ++ + P N+ S + + K ++ +F + ++ C ++ D+ ID S S+ PE+F+ F+ ++ + +R R G+ ++ I + ++ I++I + G T G A+ + LF +D VP+ LVV+TDG + + +A + + GI I ++GV + E+E+ DIA S E + V F L K I N+ + P CK D++F++ SSA+ S+ ++ M +FM+S+ K + + ++ +++F+ S KE++ +S+I + + G +G+AL F + R N + +I+I DGE D A ++ G+ ++ IG+ + L+ ++ + D+F +L N I E+C+ + K T+ D++F LD+S+SI ++ FVE +V D + + + G V + T+ ++F L+ ++ K ++ AV + G T +AL + S R + +++LVTDG +T P L
Sbjct: 235 QADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSDTLTEP---AKELKSRGISVYVVGTNIKYDR-QLQEASSKPDEKFFYQLDD------FDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVR--RHAEELKSVGVTTISVGIGN-SDRKELEKIATDPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAV----CSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNG--QSMDNFTKPAAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNV----ESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEAL-TSMKQLFVNAAKDRPHKVPQSLVVITDGESQ--DRVTEAAAEIRNDGITIFAIGVKNAV-EEEIRDIAGSNEKMFFVNNFDSL-----KVIKNDLARELCTPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFS------SETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITDGEAQD--AVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKK---TEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNL 1333 HSP 4 Score: 192.2 bits (487), Expect = 2.307e-48 Identity = 228/1000 (22.80%), Postives = 444/1000 (44.40%), Query Frame = 3
Query: 1002 DLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK---GR--EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNK--MSSTETYYPIEDMS-KMALFDTTDIAQDACQIPKPKR------ICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---THIYDDILK-KVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLV----VLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWA-----IGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKL--GGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--NFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGT--NKECSTKQLDLMFALDSS 3857
D++F++D SSS+ + + F + V+ +L I + +VG+++YSD + L L + K EI F++KL G AL ++ +K GR +G ++ +++T+G KP A KL++ G+ V +G L +I + +++ E++ + ++ ++ +I + +P R + + + DI ++D S + ++ + F I F++G N+ RF +V +++ V EF H +LK + ++ +GG T +AL + F +K+ V V++T+G S +RV +AA +++ G+ I IG+ + + E I + F +F K + + C P+ C + DI+F+VD S ++ ++ ++ M + ++ I +I +I +S ET EF L+ K +I AI+ ++ G + AL F R + + +++ TD +D++ A+ ++ GV I +G+ + + E ++ ++ + + + I IC+ Q+ C ++ D+I +D S S+ F++ FV +V + + KD + G + + +++ D+ K + +P + G T A I T Y GR + +L+++TDGA + P + V L GI + +VGVG+ + + EL IA + ++ V+ + L+ + I+ C KP DL+F++ SA+ TS F FMK + + + R+ + +++ + E Y + + I+ ++ + GK L + F + RKN + +I++ DG D E+A L+ G+K+F+IG+ + S + SP V+ F L I +++C+ KECS LD+ ++D S
Sbjct: 633 DIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQSMDNFTKP---AAKLRRSGVEVFAVGFQNINDTELDIIASHPPRKHVTNVESFLQLSNLEFRIQKRLCNEIVVKSFAVPAIARAVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSD--VRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRV--TEAAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVIKNDLARELCT------PEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSETKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKYTLPYFSASKGGRVNTKQYLIVITD---GEAQDAVGNPAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNP----QESCKKTEVADIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSY---GGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQ-ELQQIAGYPDRWFLVQNYKGLDNIH-DNITQVVCD-ESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSND--SVNEDAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQVCEPSDQPIIGKECS---LDITISVDYS 1601 HSP 5 Score: 155.992 bits (393), Expect = 2.995e-37 Identity = 169/779 (21.69%), Postives = 357/779 (45.83%), Query Frame = 3
Query: 978 QNC-NNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKK---TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT-HIY---DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK----NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPT--NQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK 3257
+ C + ++ D+ F++D S S+ ++ F +I Q+ + GV+ YSD+ + + ++ +S++E L G T AL + F++ ++ K +++++TDG + A +++ GI + IG+ +V ++ + S E + + + + + D+A++ C P+ + K DI +VD S+ + ++ F + ++Q E+G ++ + L+ F++E +EEF + Y D+I + ++ + T + +AL + ++ SK N+K+ ++++T+G + + V A +++ GV I IG+ + + L+I + F L + + C + K + DIIF++D S T + ++ + + + + + + Y +F L+ TK DI A+ L AL R F Y R ++ I++L TD T ++ + ++ LK DG+ + VG+ K P + ++N +D + I ++CD + + C+ ++LD++ ID S S+ NF F+ IV + + ++R RIG+ +++ ++ + ++DM+K+I+ I ++ T G + + GR++VP+ L+V+TDG ++ + +A+++ G+KI S+G+G + S + + S Y V+TF L+ + ++ + C+ +P I K
Sbjct: 831 EGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGGGTLTGEALTSMKQLFVNAAKDRPHKVPQSLVVITDGESQDRVTE---AAAEIRNDGITIFAIGVKNAVEEEIRDIAGSNEKMFFVNNFDSLKVI-KNDLARELC---TPEACKNMKADIVFLVDSSASINSDDYETMKEFMESMVKQAEIGPDRVQIGLIQFSSE--TKEEFPLNRYKRKDEIQSAIRGIQQLSQGTLMGEALKY-TLPYFSASKGGRVNTKQYLIVITDGEAQDAVGN--PAKAIRDHGVIIYAIGVQQANNTQLLEIAGKQEQVYYEDSFDSLAFLNKNIMFEICNPQESCKKTEVA---DIIFLLDASASITRGEFRLMQRFVEAVVNDSLVGKDNVQFGAVVYGTNPAEQFSLNTYSTKLDILKAVFSLPQVSGYTYTAKALEYTRIRFGTSYGGRPGISHILILVTDGATTEADRPNLPIVSKALKDDGIIVFAVGVGKAVPQELQQIAGYPDRWFLVQNYKGLDNIHDNITQVVCD---ESKPACSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNEDAAALRSS--GVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVA-QVCEPSDQPIIGK 1588 HSP 6 Score: 135.191 bits (339), Expect = 5.302e-31 Identity = 168/730 (23.01%), Postives = 337/730 (46.16%), Query Frame = 3
Query: 2112 TCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN-RARQVFK--IDYRA--DVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMI---CDAFKQRQDE-------C-TMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQIPQKPTI-CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF----VDNFSNLQR--YNAYISSELCDV--IKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
T +S DI+F++D S E + +++ + T + ++ +I +I YSD TEF L+ K D+ I+ L+ G G +L Q F RA V +I V+ TD +DS+ E I +K G+ + +G+ K++ + +AS + ++ + + +NMI C ++ + C T + D+++ ++ +T +G F+ +F+ +V NLD+ ++ RIG+ T+N E ++S ++ + I + ++EG T G A+ FT+ E V ++L+++T+G D ++ A + K GI + VG + +++L + +S + FY + F D E + + N I + K D++F++ SS + + F++M DF+ + V R+ + +++ + ++ + ++ + + E T + G AL R F ++ +R N +++ + A LK G+ ++G+ G + +L+ +A+ D +F + + SNLQ+ N I+ ++ + CS+ + D++F +D SSSIGP +Q F+ ++V +DI+ + +VG+V ++ + R E L+ F EKY ++ + ++ + G G AL + ++ N R V ++ V++T+G S
Sbjct: 28 TEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITD---GQAQDSIREPAIAVKNAGIILYAIGI-KDAVLSELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILESIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEG--DSSDTLTEPAKELKSRGISVYVVGTNIKY-DRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHALEFVRSTFFIEEAGSRINYGNPQFLVVITSSKSEDAVRRHAEELKSVGVTTISVGI-GNSDRKELEKIAT-DPFVFQTTGLQHISNLQQDVANVIIAEDMLQFSPVSVVPAVCSSASVADIVFLIDESSSIGPINFQLTRVFLHKVVSALDISLSNVRVGLVLYSDEPRLELKLNTFNEKYEILDFITKLPYRGGKAHTGAALDFLRKKMFTKQNGGRPHQGVQQIAVVMTNGQS 748 HSP 7 Score: 121.709 bits (304), Expect = 8.160e-27 Identity = 115/497 (23.14%), Postives = 226/497 (45.47%), Query Frame = 3
Query: 2682 CTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAI-----GNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFN-------AKTISNEACQIPQKPTICKAA--GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF--VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKR-IIFDDGATDIGEAL 4082
CT + D++ +D S S+G ENF +F+ +V D+ D+ +IG+ ++ ++S D+ K I + + G T G ++ + ++ ++ E VP++ VV+TDG A D P A+ K GI + ++G+ + ELN+IAS + Y+V F L+ + T+ A Q Q +C+ A D++ ++ S+ F++ +F+ L V R+ ++ +N ++ + + + E SI++M + G+AL + S ++ + +II+ +G+ +D E A LK GI V+ +G K ++ + PD+ F +D+F + + + LC I + S + D++F +DSS+S+G ++Q DF+ I+ +++ N ++G+ ++ + EF L+ + K ++ +K + G G AL
Sbjct: 27 CTEASIADIVFLMDGSWSIGTENFITMKNFLYTLVNGFDVGLDKIQIGLIQYSDNARTEFFLNSYSNKEDVLKYIQNLKYKGGGTKTGLSLEFMLTQHFSEAAGSRAAEGVPQIAVVITDGQAQDSIREP---AIAVKNAGIILYAIGIKDAVL-SELNEIASDPDDKHVYSVADFNALQSISQNMIQVLCTTVEEAARQTGQIAQVCRTANQADIVLLVESTTRMGDATFEKAKNFLYDLVSNLDVGINKIRIGLVTYNDETNPEFLLNSYSSKTEILE-SIQNMKYVEGYTYTGRALEYVNTTYFTQAAGSRFEESVAQILIIVTEGDSSD--TLTEPAKELKSRGISVYVVGTNIKYDRQLQEASSKPDEKFFYQLDDFDDSENVTEQLLKNLCFSIDLNIQVYSKRYADVVFLVDSSTSMGTIFFQKMKDFIIHIINQLNVGINKHRIGLAQYSGLPQTEFLLNHYETKEEILKHIKETFTYRGGPLKTGHAL 516 HSP 8 Score: 59.3066 bits (142), Expect = 7.003e-8 Identity = 53/215 (24.65%), Postives = 101/215 (46.98%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEF--ISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQI---PKPKRICSAKLDIALIVDCSSQVQL 1610
C+++QLDL+F++D S+S+ + + F ++ S I ++GV YS + L + ++++K + + L+ T+ LR + F + GR+ + +I++TDG N + +A L+ G+++ IG+GL + + S + Y +E + +AQ C+ P + CS LDI + VD S + L
Sbjct: 1398 CSHEQLDLVFLIDGSASITSSNFTSAKTFMKEIVDSFTISENRVRIGVAQYSANPKKEFFLNEYYSSSDMKKQIDSISQLKATTYTGKGLRFVKQFFDPANGGRKNVPQYLIVMTDGMSNDSVNE---DAAALRSSGVKIFSIGIGLRNSFELVMIAGSPKNVYEVETFQALDSIKRQIVAQ-VCEPSDQPIIGKECS--LDITISVDYSRRAML 1606 HSP 9 Score: 57.7658 bits (138), Expect = 1.874e-7 Identity = 40/153 (26.14%), Postives = 79/153 (51.63%), Query Frame = 3
Query: 117 ALDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNST--LYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
A+ + C+ + DI ++D S SI +F+ + + +S F + N R + Y D +TEF ++H + L A+ ++GG ++ G AL S L+ + RP ++ + L++++DG + D V + A +I+ G+TI+ + +
Sbjct: 828 AVKEGCVDTEEADIYFLIDGSGSIYPEDFEDMKKFMIELISMFQVGANRVRFGVVQYSDVRRTEFFISEHNTQKMLKDAISQIEQLGG-GTLTGEALTSMKQLFVNAAKDRP-HKVPQSLVVITDGESQDR--VTEAAAEIRNDGITIFAIGV 976
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: COL6A6 (Gametogenetin-binding protein 2 [Source:UniProtKB/Swiss-Prot;Acc:Q6DFP9]) HSP 1 Score: 167.548 bits (423), Expect = 6.980e-41 Identity = 246/1142 (21.54%), Postives = 491/1142 (42.99%), Query Frame = 3
Query: 954 YIEISTPDQNCNNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFV-SKLELLEGATFISNALRRSGEEFISKGREGAKKT----VILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTT------DIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDK-------ALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-----------------PFLR-------QDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVP-----KVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEAC-QIPQKPTICK--AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRK--NATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPD-KALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-----NSTRKEVPKVIVLVTDG 4157
+++IS + + DL+F++D+SS+L + Q +I SL + +VG+ Y+D L++ L + + K + + +KL G+ + NAL ++ E+F + + K +++V G+ +P +E L++ G+R++ +GL + + SST + ++ + TT D+ Q +P P + DI I+D S + E N F + + N LV + ++A EF + + K L+ + T + + ALN K S K++ +L+T+ S + V AA+ L++ V +G+ + A + ++ ++++P +L+ +D+ + ++ C T A DI +VD+S T + + +++ + ++ +I + + YSD + E + F+K ++ A++ LR G G + A+N +QV + DV +++ TD +DS+ EA EI++G GV +G+ + E ++ + + + K+ I IC C D++ +D S+ +G + F F+ +V ++ + F++ + + + + I+ + + G A+ N DY FT+ + P K+L+++TD N + ++ A + G+ I S+G G ++KEL +I+ Y ++F L+ TI N+ +I CK D++FV+ SS + +K+M +FM SL + V +N + LK++ N + + Y N + I TT + A+ ++ F K +RK + V++I DG+ D+++ E A L+++GI ++ IG+ + + ++L+++A + K V NFS LQ +S +C+ +C +Q DL+F +D SS+ + ++ +FV ++ D ++ + V ++ +E D TE+ + + + I G IG AL K+ + N + V +++V++T G
Sbjct: 13 WLQISFTQKAAVPEYADLVFLVDSSSNLGEKGFAQVKTLITRIISSLPVGPDKYRVGLAQYNDDLDIGFLLNTYKGKGPVISHIKNKLAYKGGSLKLGNALTKAHEKFFKEVSDRDKSVYPPVLVVVASGSSLDEVTQPALE---LQQDGVRIVSVGLQTAPLSQLQAIASSTRHVLQVSNIRDIPAVSTTLIDSIKDVVQSKTVVPTPSEFTA---DIVFIIDTSRH-KPSESENLKKFLSDVVSTLPISENCAHVHLVKYGSKA----EFLAPLETGVNKSRVLEAIKSITPMKEEAANTGHALNFTRTEVVADPKTSRKGQGIKQISILITHSPSADNV--TDAAHLLRQDRVTSFALGIAE-AKKNQMDQIASYPPDSYTISLKGFSDLSTQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEA---------ADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSD-SPREIPIGRDFSKDELKSALQNLRQLGGGTNTGKAINFTQQVITKTENTRPGDVPVYLIVLTD---GESQDSVKEAAEIVRGHGVNTYAIGVKGYNKTQLLEITGAEHRVHLAHDFDSLKHIKDVIAQQICFG-----KACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNSIKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVADY-FTEAKGARPSSKVSKILLLITD--NPSQDEVKVPADSLRSNGLIIYSIG-GFSANKKELQEISGKITPY-YQSFDKLQ-----TIENDLLFRICNPEEECKRIEVADIVFVIDSSGSIDYTEYKEMQNFMVSLVNKSAVGPDNVQFGALKYSDYN-TELFYLNRYTNKVDIINHINKDTTQGGNTYTAGAVRFSKEFFTEKHGSRKARGVPQIVMVITDGDSHDKDKLNETARQLEQEGIIIYAIGID-QANTNELETLAGTEGKWFMVANFSGLQDILVQVSEAMCN-----KTDCKVEQADLLFLIDGSSNTSEETFREIKNFVVSVMDDFNVGPVNVHIAVSQYSESCIREINFDYSTERGTLKNEIINIRKTKGRRHIGAALDFTKSTVYSPSSDNRLNQGVKQLLVVITAG 1105 HSP 2 Score: 98.2117 bits (243), Expect = 9.270e-20 Identity = 180/794 (22.67%), Postives = 356/794 (44.84%), Query Frame = 3
Query: 891 TSVKQTTKSIPFETKRTTTSNYIEISTPDQNC-NNQQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQD--ACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT---HIYDD-ILKKVESLKHVGGFTRIDKAL-NLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRA----DVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED-----VPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTD 3185
T V+ K+I E + T +Y Q C + + D+ ++D S S+ QE+ F +++ I + V ++YSD +P+G +K E++ + L L G T A+ + ++ I+K R G ++V ++ ++K AE ++ G+ IG+ K QL +++ E + FD+ +D A QI K DI +VD SS + ++ F K + + E+G+ +F+ V F+N ++EEF H + I ++++ +G + AL N+A ++ ++K ++ ++++L+T+ S + V+ A L+ +G+ I +IG G A+++EL+ +S P+ Q F + +++ + C K + DI+FV+D S + +++ M++ + + + + YSD T F L+ K DI I + + G A+ +++ F + + V +IV++ TD ++ KD L+E A L+ +G+ I +G+ + + N E LA + + + I + +A + D C +++ D++ ID S++ E F+ +FV ++ + ++ I + ++ + D + E ++ EI I +G IG A+ +++ ++ V ++LVV+T G A+D P A++ + G+ I +VG+G I + +L I S E K +TD
Sbjct: 393 TQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEAADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSDS-PREIPIGRDFSKDELKSALQNLRQLGGGTNTGKAINFT-QQVITKTENTRPGDVPVYLIVLTDGESQDSVKEA--AEIVRGHGVNTYAIGVK-GYNKTQLLEITGAEHRVHLAHD-----FDSLKHIKDVIAQQICFGKACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNS--IKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVA--DYFTEAKGARPSSKVSKILLLITDNPSQDEVKV--PADSLRSNGLIIYSIG-GFSANKKELQEISGKITPYY-QSFDKLQTIENDLLFRICNPEEECKRIEVA---DIVFVIDSSGSIDYTEYKEMQNFMVSLVNKSAVGPDNVQFGALKYSDYNTELFYLNRYTNKVDIINHINKDTTQGGNTYTAGAVRFSKEFFTEKHGSRKARGVPQIVMVITDG-DSHDKDKLNETARQLEQEGIIIYAIGIDQANT-NELETLA---GTEGKWFMVANFSGLQDILVQVSEAMCNKTD-CKVEQADLLFLIDGSSNTSEETFREIKNFVVSVMDDFNVGPVNVHIAVSQYSESCIREINFDYSTERGTLKNEIINIRKTKGRRHIGAALDFTKSTVYSPSSDNRLNQGVKQLLVVITAGNASDQVARPA-KALRDR--GVDIYAVGIG-NICKTQLTQITGSPE----KIYTD 1151 HSP 3 Score: 89.7373 bits (221), Expect = 3.866e-17 Identity = 144/724 (19.89%), Postives = 311/724 (42.96%), Query Frame = 3
Query: 2127 GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIK-QLRSNGNGGDLNTALNRARQVF--KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXX-MICDAFKQRQDECTMKKL--DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPK------------VLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVG-QQISEKELNDIAS-SGEFYNV--KTFTDLE---KFNAKTISNEA----------CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALT-SARVLFKSDTRKNATKTVIIII--DGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS----TRKEVPKVIVLVTDGVS 4163
D++F+VD S++ + + +++L+ + SL + R+ + Y+D+ F L+ K + IK +L G L AL +A + F ++ R VL ++ + A L+ DGV+I+ VGL + +P + + +AS + + + I D + + T + D++ ID S E+ ++ F++ +V L + ++ + + + S+ P+++ + + + I I ++ A G+A ++ T+ D PK +L+ + A+++ ++ + L ++ S +G + + +++ IAS + Y + K F+DL ++ K I NE + Q+ + A D+ ++ S + T++ F++M F+K L ++ + E V + ++ R I F +D +S+++++ G GKA+ + +V+ K++ + V +I+ DGE D + E A +++ G+ + IG+ G L+ + + +F +L+ I+ ++C K C D++F +DSSS +G D + F++ +V ++ + V F+ ++EF ++ K A+ ++ + G AL + + +V K+++L+TD S
Sbjct: 28 ADLVFLVDSSSNLGEKGFAQVKTLITRIISSLPVGPDKYRVGLAQYNDDLDIGFLLNTYKGKGPVISHIKNKLAYKGGSLKLGNALTKAHEKFFKEVSDRDKSVYPPVLVVVASGSSLDEVTQPALELQQDGVRIVSVGL-QTAPLSQLQAIASSTRHVLQVSNIRDIPAVSTTLIDSIKDVVQSKTVVPTPSEFTADIVFIIDTSRHKPSESENLK-KFLSDVVSTLPISENCAHVHLVKYGSKAEFLAPLETGVNKSRVLEAIKSITPMKEEAA---NTGHALNFTRTEVVAD-PKTSRKGQGIKQISILITHSPSADNVTDAAHL------LRQDRVTSFALGIAEAKKNQMDQIASYPPDSYTISLKGFSDLSTQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEAADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSDSPREIPIGRDFSKD--ELKSALQNLRQLGGGTNTGKAINFTQQVITKTENTRPGDVPVYLIVLTDGESQDSVK--EAAEIVRGHGVNTYAIGVKGYNKTQLLEITGAEHRVHLAHDFDSLKHIKDVIAQQIC-----FGKACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNSIKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVADYFTEAKGARPSSKVSKILLLITDNPS 730
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Match: COL6A6 (Gametogenetin-binding protein 2 [Source:UniProtKB/Swiss-Prot;Acc:Q6DFP9]) HSP 1 Score: 157.918 bits (398), Expect = 3.343e-38 Identity = 248/1127 (22.01%), Postives = 488/1127 (43.30%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFV-SKLELLEGATFISNALRRSGEEFISKGREGAKKT----VILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTT--DIAQDACQIP--KPKRICSAKL--DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDK-------ALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-----------------PFLR-------QDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKGREDVP-----KVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEAC-QIPQKPTICK--AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRK--NATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-----NSTRKEVPKVIVLVTDG 4157
DL+F++D+SS+L + Q +I SL + +VG+ Y+D L++ L + + K + + +KL G+ + NAL ++ E+F + + K +++V G+ +P +E L++ G+R++ +GL + + SST + ++ + TT D +D Q C + DI I+D S + E N F + + N LV + ++A EF + + K L+ + T + + ALN K S K++ +L+T+ S + V AA+ L++ V +G+ + A + ++ ++++P +L+ +D+ + ++ C T A DI +VD+S T + + +++ + ++ +I + + YSD + E + F+K ++ A++ LR G G + A+N +QV + DV +++ TD +DS+ EA EI++G GV +G+ + E ++ + + + K+ I IC F + C D++ +D S+ +G + F F+ +V ++ + F++ + + + + I+ + + GN A G A+ N DY FT+ + P K+L+++TD N + ++ A + G+ I S+G G ++KEL +I+ Y ++F L+ TI N+ +I CK D++FV+ SS + +K+M +FM SL + V +N + LK++ N + + Y N + I TT + A+ ++ F K +RK + V++I DG+ D+++ E A L+++GI ++ IG+ + + ++L+++A + F V NFS LQ +S +C+ +C +Q DL+F +D SS+ + ++ +FV ++ D ++ + V ++ +E D TE+ + + + I G IG AL K+ + N + V +++V++T G
Sbjct: 4 DLVFLVDSSSNLGEKGFAQVKTLITRIISSLPVGPDKYRVGLAQYNDDLDIGFLLNTYKGKGPVISHIKNKLAYKGGSLKLGNALTKAHEKFFKEVSDRDKSVYPPVLVVVASGSSLDEVTQPALE---LQQDGVRIVSVGLQTAPLSQLQAIASSTRHVLQVSNIRDIPAVSTTLIDSIKDVVQTAGTADNTACDQEFTADIVFIIDTSRH-KPSESENLKKFLSDVVSTLPISENCAHVHLVKYGSKA----EFLAPLETGVNKSRVLEAIKSITPMKEEAANTGHALNFTRTEVVADPKTSRKGQGIKQISILITHSPSADNV--TDAAHLLRQDRVTSFALGIAE-AKKNQMDQIASYPPDSYTISLKGFSDLSTQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEA---------ADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSD-SPREIPIGRDFSKDELKSALQNLRQLGGGTNTGKAINFTQQVITKTENTRPGDVPVYLIVLTDG---ESQDSVKEAAEIVRGHGVNTYAIGVKGYNKTQLLEITGAEHRVHLAHDFDSLKHIKDVIAQQIC--FGK---ACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNSIKEEFQFNKHATKNAIWDSIDNMNLM-GNVAKTGNALANVADY-FTEAKGARPSSKVSKILLLITD--NPSQDEVKVPADSLRSNGLIIYSIG-GFSANKKELQEISGKITPY-YQSFDKLQ-----TIENDLLFRICNPEEECKRIEVADIVFVIDSSGSIDYTEYKEMQNFMVSLVNKSAVGPDNVQFGALKYSDYN-TELFYLNRYTNKVDIINHINKDTTQGGNTYTAGAVRFSKEFFTEKHGSRKARGVPQIVMVITDGDSHDKDKLNETARQLEQEGIIIYAIGID-QANTNELETLAGTEGKWFMVANFSGLQDILVQVSEAMCN-----KTDCKVEQADLLFLIDGSSNTSEETFREIKNFVVSVMDDFNVGPVNVHIAVSQYSESCIREINFDYSTERGTLKNEIINIRKTKGRRHIGAALDFTKSTVYSPSSDNRLNQGVKQLLVVITAG 1083 HSP 2 Score: 98.2117 bits (243), Expect = 6.825e-20 Identity = 181/795 (22.77%), Postives = 356/795 (44.78%), Query Frame = 3
Query: 891 TSVKQTTKSIPFETKRTTTSNYIEISTPDQNC-NNQQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGL-GLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQD--ACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT---HIYDD-ILKKVESLKHVGGFTRIDKAL-NLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRA----DVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED-----VPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTD 3185
T V+ K+I E + T +Y Q C + + D+ ++D S S+ QE+ F +++ I + V ++YSD +P+G +K E++ + L L G T A+ + ++ I+K R G ++V ++ ++K AE ++ G+ IG+ G + K QL +++ E + FD+ +D A QI K DI +VD SS + ++ F K + + E+G+ +F+ V F+N ++EEF H + I ++++ +G + AL N+A ++ ++K ++ ++++L+T+ S + V+ A L+ +G+ I +IG G A+++EL+ +S P+ Q F + +++ + C K + DI+FV+D S + +++ M++ + + + + YSD T F L+ K DI I + + G A+ +++ F + + V +IV++ TD ++ KD L+E A L+ +G+ I +G+ + + N E LA + + + I + +A + D C +++ D++ ID S++ E F+ +FV ++ + ++ I + ++ + D + E ++ EI I +G IG A+ +++ ++ V ++LVV+T G A+D P A + G+ I +VG+G I + +L I S E K +TD
Sbjct: 371 TQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEAADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSD-SPREIPIGRDFSKDELKSALQNLRQLGGGTNTGKAINFT-QQVITKTENTRPGDVPVYLIVLTDGESQDSVKEA--AEIVRGHGVNTYAIGVKGYN--KTQLLEITGAEHRVHLAHD-----FDSLKHIKDVIAQQICFGKACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNS--IKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVA--DYFTEAKGARPSSKVSKILLLITDNPSQDEVKV--PADSLRSNGLIIYSIG-GFSANKKELQEISGKITPYY-QSFDKLQTIENDLLFRICNPEEECKRIEVA---DIVFVIDSSGSIDYTEYKEMQNFMVSLVNKSAVGPDNVQFGALKYSDYNTELFYLNRYTNKVDIINHINKDTTQGGNTYTAGAVRFSKEFFTEKHGSRKARGVPQIVMVITDG-DSHDKDKLNETARQLEQEGIIIYAIGIDQANT-NELETLA---GTEGKWFMVANFSGLQDILVQVSEAMCNKTD-CKVEQADLLFLIDGSSNTSEETFREIKNFVVSVMDDFNVGPVNVHIAVSQYSESCIREINFDYSTERGTLKNEIINIRKTKGRRHIGAALDFTKSTVYSPSSDNRLNQGVKQLLVVITAGNASDQVARP---AKALRDRGVDIYAVGIG-NICKTQLTQITGSPE----KIYTD 1129 HSP 3 Score: 86.6557 bits (213), Expect = 2.857e-16 Identity = 145/727 (19.94%), Postives = 313/727 (43.05%), Query Frame = 3
Query: 2127 GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIK-QLRSNGNGGDLNTALNRARQVF--KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXX-----XXXXXXXXXXXXMICDAFKQRQDECTMK-KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPK------------VLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVG-QQISEKELNDIAS-SGEFYNV--KTFTDLE---KFNAKTISNE----------ACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALT-SARVLFKSDTRKNATKTVIIII--DGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS----TRKEVPKVIVLVTDGVS 4163
D++F+VD S++ + + +++L+ + SL + R+ + Y+D+ F L+ K + IK +L G L AL +A + F ++ R VL ++ + A L+ DGV+I+ VGL + +P + + +AS + + + IK+++ A C + D++ ID S E+ ++ F++ +V L + ++ + + + S+ P+++ + + + I I ++ A G+A ++ T+ D PK +L+ + A+++ ++ + L ++ S +G + + +++ IAS + Y + K F+DL ++ K I NE + Q+ + A D+ ++ S + T++ F++M F+K L ++ + E V + ++ R I F +D +S+++++ G GKA+ + +V+ K++ + V +I+ DGE D + E A +++ G+ + IG+ G L+ + + +F +L+ I+ ++C K C D++F +DSSS +G D + F++ +V ++ + V F+ ++EF ++ K A+ ++ + G AL + + +V K+++L+TD S
Sbjct: 3 ADLVFLVDSSSNLGEKGFAQVKTLITRIISSLPVGPDKYRVGLAQYNDDLDIGFLLNTYKGKGPVISHIKNKLAYKGGSLKLGNALTKAHEKFFKEVSDRDKSVYPPVLVVVASGSSLDEVTQPALELQQDGVRIVSVGL-QTAPLSQLQAIASSTRHVLQVSNIRDIPAVSTTLIDSIKDVVQTAGTADNTACDQEFTADIVFIIDTSRHKPSESENLK-KFLSDVVSTLPISENCAHVHLVKYGSKAEFLAPLETGVNKSRVLEAIKSITPMKEEAA---NTGHALNFTRTEVVAD-PKTSRKGQGIKQISILITHSPSADNVTDAAHL------LRQDRVTSFALGIAEAKKNQMDQIASYPPDSYTISLKGFSDLSTQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEAADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSDSPREIPIGRDFSKD--ELKSALQNLRQLGGGTNTGKAINFTQQVITKTENTRPGDVPVYLIVLTDGESQDSVK--EAAEIVRGHGVNTYAIGVKGYNKTQLLEITGAEHRVHLAHDFDSLKHIKDVIAQQIC-----FGKACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNSIKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVADYFTEAKGARPSSKVSKILLLITDNPS 708
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a6 (collagen, type VI, alpha 6 [Source:MGI Symbol;Acc:MGI:2444259]) HSP 1 Score: 225.713 bits (574), Expect = 2.455e-59 Identity = 270/1130 (23.89%), Postives = 504/1130 (44.60%), Query Frame = 3
Query: 975 DQNCNNQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSK-LELLEGATFISNALRRSGEEFIS---KGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF---------DTT--------DIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFL-------------RQDFXXXXXXXXXXXXXXCKTTPAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN---RARQVFKIDYRADVAKIVVLFTDKLPTNQKDS-LSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-KG-REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDS-GFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN----ELLNSTRKEVPKVIVLVTDGVS 4163
+Q+ + D++F++D+S L L + F + +I SL I+ +V + YSD L LG+ +N+ + + K + G+ I NAL+ + + S GR+ + ILV + S E A+ L++ G+++I +G+ ++ L M++++ ++ + +++F D T DI +ACQ P + D+ ++D + + + F I ++ N R LV+++NE V + + ++L++++ L G AL + + + S++ + VL+T+ S + V +AA +L+ GV I T+G+ + A+ +EL+ +++ P Q F ++ C + DI ++D S + +++ + + NI+ R+ + Y+D EF + + K D+ AI+ +R G + ALN + Q K + + V +V+ T+ + +DS L A L+ + +++ +G+ + + E +K + + + + Q+ IC ++ C K D++ +D S S+GPENF F+ +V + DR +IG+ F+ E +++ +D+ I+++ I T G A+ + Y KG R +V K L+++TDG A DI P I+ K G+ I SVGV G +++ L +I+ E + V+ F L+ + C P++ D++FV+ SS + + + M DFM L K V K R LK+ Y+D G K + + + M +T + +AL + +F S K + +I+I DGE D + A L+ GI V +G+ G S L S DK FV+ F L+ + +S+ +C+ + +C +++DL+F +D S+SI PD +Q F+ +V+D D++ N ++GV F+ R EF L FT + + + ++ I G T IG+ALR+ K ++ + P+V++++TDG S
Sbjct: 17 NQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEA-AKALREDGVKIISVGVQ-KASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGP-------SVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNV--TKAAVNLRREGVTIFTMGI-EGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGM---SRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEIC-----AEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKE---GVIIYSVGVFGSNVTQ--LEEISGKPEMVFYVENFDILQHIEDDLVLG-ICS-PREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNT---YTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCN---SSKVDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRS 1113 HSP 2 Score: 179.104 bits (453), Expect = 9.176e-45 Identity = 230/981 (23.45%), Postives = 436/981 (44.44%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQE-----LAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGR-----EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIE-------DMSKMALFDTTDIAQDACQIPKP-----------KRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALN----LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID--YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELC 3785
D++F+LD +A+ QE AF I +L I +VG++ YS+ + L + NKTE+ + + L G + ALR++ +E S R +G + +LVT A K V L++ G+ + +G+ D+L K++S +P E + S++A + T + + QI K C + + DI L++D S Q ++ F + F + +K R V +A+ +E E + + D+ K +E+++ +GG T ALN L R+ + +V+LTNG+S + V + A+ L+E + + IG+ + + +I + +F +++ + V+ C + C DI+F+VD S E + ++ M + I +I ++ +S E EF+L+ ++ DI AI ++ G +AL Q F D R +V K ++L TD +D + + I L+ +GV I VG+ ++ E + +EN + + ++ + IC ++EC ++ LDV+ ID S S+ + + I DF+ +V+ D+ K++ R G + + + +D ++ + + GNT A+ +D++FT+ R + VP+VL+V+TDG + E +A + GI +++VG+ S + L SS ++Y V+TF L+ + +S C + + DL+F+M S + + F++M F+ S+ + V R+ + +F+ R ++ F + + + IE + IG AL + F+ D +R NA + ++++ DG D E + A L+ G+ ++++G+ + ++ + +K L V NF L++ I +C
Sbjct: 228 DVVFLLD----MAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAV---NLRREGVTIFTMGIE-GANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSV--LGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEIC------AEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITD---GEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSP----REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALA-FSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSD-VSASVCNSSKVDCEIEKV-DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGE-REISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQD--EVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNIC 1175 HSP 3 Score: 66.2402 bits (160), Expect = 3.157e-10 Identity = 48/179 (26.82%), Postives = 88/179 (49.16%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPT-SEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
DI +++D S S +F +++ + F I P+ RV + Y DT EF +K+++ P L A+ R++GG+++ G ALN TL + + S++ L++L++G + D SV A K++E+ + ++ + + L +K V EF +R +V IC
Sbjct: 435 DIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNT-GAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRD--SVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEIC 610 HSP 4 Score: 58.151 bits (139), Expect = 9.662e-8 Identity = 40/142 (28.17%), Postives = 72/142 (50.70%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM--RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
D+ ++D S+SI +FQ + + F + N R+ + D+ ++EFL T ++ + +++ G + + G AL Y P+M R + ++LL+L+DG + DE V A +++ KGV IY+V IG
Sbjct: 999 DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHI-GDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDE--VAQAAEELRHKGVDIYSVGIG 1137
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a6 (collagen, type VI, alpha 6 [Source:MGI Symbol;Acc:MGI:2444259]) HSP 1 Score: 226.483 bits (576), Expect = 4.717e-59 Identity = 271/1132 (23.94%), Postives = 506/1132 (44.70%), Query Frame = 3
Query: 975 DQNCNNQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSK-LELLEGATFISNALRRSGEEFISKGREGAKK----TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF---------DTT--------DIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFL-------------RQDFXXXXXXXXXXXXXXCKTTPAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN---RARQVFKIDYRADVAKIVVLFTDKLPTNQKDS-LSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-KG-REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKELNDIASSGE--FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDS-GFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECK----NELLNSTRKEVPKVIVLVTDGVS 4163
+Q+ + D++F++D+S L L + F + +I SL I+ +V + YSD L LG+ +N+ + + K + G+ I NAL+ + + S G K +++V A++ + E A+ L++ G+++I +G+ ++ L M++++ ++ + +++F D T DI +ACQ P + D+ ++D + + + F I ++ N R LV+++NE V + + ++L++++ L G AL + + + S++ + VL+T+ S + V +AA +L+ GV I T+G+ + A+ +EL+ +++ P Q F ++ C + DI ++D S + +++ + + NI+ R+ + Y+D EF + + K D+ AI+ +R G + ALN + Q K + + V +V+ T+ + +DS L A L+ + +++ +G+ + + E +K + + + + Q+ IC ++ C K D++ +D S S+GPENF F+ +V + DR +IG+ F+ E +++ +D+ I+++ I T G A+ + Y KG R +V K L+++TDG A DI P I+ K G+ I SVGV G +++ L +I+ E FY V+ F L+ + C P++ D++FV+ SS + + + M DFM L K V K R LK+ Y+D G K + + + M +T + +AL + +F S K + +I+I DGE D + A L+ GI V +G+ G S L S DK FV+ F L+ + +S+ +C+ K +C +++DL+F +D S+SI PD +Q F+ +V+D D++ N ++GV F+ R EF L FT + + + ++ I G T IG+ALR+ K ++ + P+V++++TDG S
Sbjct: 17 NQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDV--EEAAKALREDGVKIISVGV-QKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGP-------SVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNV--TKAAVNLRREGVTIFTMGI-EGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGM---SRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEIC-----AEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKE---GVIIYSVGVFGSNVTQ--LEEISGKPEMVFY-VENFDILQHIEDDLVLG-ICS-PREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNT---YTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSK---VDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRS 1113 HSP 2 Score: 179.104 bits (453), Expect = 1.702e-44 Identity = 237/1012 (23.42%), Postives = 448/1012 (44.27%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQE-----LAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGR-----EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIE-------DMSKMALFDTTDIAQDACQIPKP-----------KRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALN----LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID--YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF---NAKTISNEA---CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSS 3860
D++F+LD +A+ QE AF I +L I +VG++ YS+ + L + NKTE+ + + L G + ALR++ +E S R +G + +LVT A K V L++ G+ + +G+ D+L K++S +P E + S++A + T + + QI K C + + DI L++D S Q ++ F + F + +K R V +A+ +E E + + D+ K +E+++ +GG T ALN L R+ + +V+LTNG+S + V + A+ L+E + + IG+ + + +I + +F +++ + V+ C + C DI+F+VD S E + ++ M + I +I ++ +S E EF+L+ ++ DI AI ++ G +AL Q F D R +V K ++L TD +D + + I L+ +GV I VG+ ++ E + +EN + + ++ + IC ++EC ++ LDV+ ID S S+ + + I DF+ +V+ D+ K++ R G + + + +D ++ + + GNT A+ +D++FT+ R + VP+VL+V+TDG + E +A + GI +++VG+ S + L SS ++Y V+TF L+ + ++ N + C+I + DL+F+M S + + F++M F+ S+ + V R+ + +F+ R ++ F + + + IE + IG AL + F+ D +R NA + ++++ DG D E + A L+ G+ ++++G+ + ++ + +K L V NF L++ I +C G C +D++ D SS
Sbjct: 228 DVVFLLD----MAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAV---NLRREGVTIFTMGI-EGANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSV--LGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAE------EACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITD---GEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSP----REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALA-FSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKV--------DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGE-REISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQD--EVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNIC--TSGGESNCF---VDVVVGFDISS 1195 HSP 3 Score: 66.2402 bits (160), Expect = 3.862e-10 Identity = 48/180 (26.67%), Postives = 88/180 (48.89%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRP-TSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICV 686
DI +++D S S +F +++ + F I P+ RV + Y DT EF +K+++ P L A+ R++GG+++ G ALN TL + + S++ L++L++G + D SV A K++E+ + ++ + + L +K V EF +R +V IC
Sbjct: 435 DIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNT-GAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRD--SVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICA 611 HSP 4 Score: 57.7658 bits (138), Expect = 1.604e-7 Identity = 40/142 (28.17%), Postives = 72/142 (50.70%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM--RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
D+ ++D S+SI +FQ + + F + N R+ + D+ ++EFL T ++ + +++ G + + G AL Y P+M R + ++LL+L+DG + DE V A +++ KGV IY+V IG
Sbjct: 999 DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHI-GDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDE--VAQAAEELRHKGVDIYSVGIG 1137
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a6 (collagen, type VI, alpha 6 [Source:MGI Symbol;Acc:MGI:2444259]) HSP 1 Score: 226.483 bits (576), Expect = 5.642e-59 Identity = 271/1132 (23.94%), Postives = 506/1132 (44.70%), Query Frame = 3
Query: 975 DQNCNNQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSK-LELLEGATFISNALRRSGEEFISKGREGAKK----TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF---------DTT--------DIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFL-------------RQDFXXXXXXXXXXXXXXCKTTPAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN---RARQVFKIDYRADVAKIVVLFTDKLPTNQKDS-LSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-KG-REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKELNDIASSGE--FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDS-GFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECK----NELLNSTRKEVPKVIVLVTDGVS 4163
+Q+ + D++F++D+S L L + F + +I SL I+ +V + YSD L LG+ +N+ + + K + G+ I NAL+ + + S G K +++V A++ + E A+ L++ G+++I +G+ ++ L M++++ ++ + +++F D T DI +ACQ P + D+ ++D + + + F I ++ N R LV+++NE V + + ++L++++ L G AL + + + S++ + VL+T+ S + V +AA +L+ GV I T+G+ + A+ +EL+ +++ P Q F ++ C + DI ++D S + +++ + + NI+ R+ + Y+D EF + + K D+ AI+ +R G + ALN + Q K + + V +V+ T+ + +DS L A L+ + +++ +G+ + + E +K + + + + Q+ IC ++ C K D++ +D S S+GPENF F+ +V + DR +IG+ F+ E +++ +D+ I+++ I T G A+ + Y KG R +V K L+++TDG A DI P I+ K G+ I SVGV G +++ L +I+ E FY V+ F L+ + C P++ D++FV+ SS + + + M DFM L K V K R LK+ Y+D G K + + + M +T + +AL + +F S K + +I+I DGE D + A L+ GI V +G+ G S L S DK FV+ F L+ + +S+ +C+ K +C +++DL+F +D S+SI PD +Q F+ +V+D D++ N ++GV F+ R EF L FT + + + ++ I G T IG+ALR+ K ++ + P+V++++TDG S
Sbjct: 17 NQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDV--EEAAKALREDGVKIISVGV-QKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGP-------SVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNV--TKAAVNLRREGVTIFTMGI-EGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGM---SRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEIC-----AEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKE---GVIIYSVGVFGSNVTQ--LEEISGKPEMVFY-VENFDILQHIEDDLVLG-ICS-PREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNT---YTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSK---VDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRS 1113 HSP 2 Score: 178.718 bits (452), Expect = 2.213e-44 Identity = 237/1012 (23.42%), Postives = 448/1012 (44.27%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQE-----LAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGR-----EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIE-------DMSKMALFDTTDIAQDACQIPKP-----------KRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALN----LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID--YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF---NAKTISNEA---CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSS 3860
D++F+LD +A+ QE AF I +L I +VG++ YS+ + L + NKTE+ + + L G + ALR++ +E S R +G + +LVT A K V L++ G+ + +G+ D+L K++S +P E + S++A + T + + QI K C + + DI L++D S Q ++ F + F + +K R V +A+ +E E + + D+ K +E+++ +GG T ALN L R+ + +V+LTNG+S + V + A+ L+E + + IG+ + + +I + +F +++ + V+ C + C DI+F+VD S E + ++ M + I +I ++ +S E EF+L+ ++ DI AI ++ G +AL Q F D R +V K ++L TD +D + + I L+ +GV I VG+ ++ E + +EN + + ++ + IC ++EC ++ LDV+ ID S S+ + + I DF+ +V+ D+ K++ R G + + + +D ++ + + GNT A+ +D++FT+ R + VP+VL+V+TDG + E +A + GI +++VG+ S + L SS ++Y V+TF L+ + ++ N + C+I + DL+F+M S + + F++M F+ S+ + V R+ + +F+ R ++ F + + + IE + IG AL + F+ D +R NA + ++++ DG D E + A L+ G+ ++++G+ + ++ + +K L V NF L++ I +C G C +D++ D SS
Sbjct: 228 DVVFLLD----MAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAV---NLRREGVTIFTMGI-EGANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSV--LGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAE------EACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITD---GEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSP----REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALA-FSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKV--------DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGE-REISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQD--EVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNIC--TSGGESNCF---VDVVVGFDISS 1195 HSP 3 Score: 66.2402 bits (160), Expect = 3.550e-10 Identity = 48/180 (26.67%), Postives = 88/180 (48.89%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRP-TSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICV 686
DI +++D S S +F +++ + F I P+ RV + Y DT EF +K+++ P L A+ R++GG+++ G ALN TL + + S++ L++L++G + D SV A K++E+ + ++ + + L +K V EF +R +V IC
Sbjct: 435 DIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNT-GAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRD--SVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICA 611 HSP 4 Score: 57.7658 bits (138), Expect = 1.591e-7 Identity = 40/142 (28.17%), Postives = 72/142 (50.70%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM--RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
D+ ++D S+SI +FQ + + F + N R+ + D+ ++EFL T ++ + +++ G + + G AL Y P+M R + ++LL+L+DG + DE V A +++ KGV IY+V IG
Sbjct: 999 DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHI-GDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDE--VAQAAEELRHKGVDIYSVGIG 1137
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a4 (collagen, type VI, alpha 4 [Source:MGI Symbol;Acc:MGI:1915803]) HSP 1 Score: 171.785 bits (434), Expect = 2.768e-42 Identity = 238/1124 (21.17%), Postives = 486/1124 (43.24%), Query Frame = 3
Query: 1086 SLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-----REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTET-----YYP-IEDMSKMA------LFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANE--AVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL-------RSNGNGGDLNTALNRA--RQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLS--------KNSPYNFYENL-AXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMK-KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFI--EGNTAIGWAIGNATDYLF-----TKGREDVPKVLVVLTDGANDIGESPQISAVKA-----KLGGIKIISVGVGQQISEKELNDIASSGEFYN---VKTFTDLEKFNAKTISNEACQ------------IPQKPTICKAAG-------DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKV---IVLVTDGVSTGGLSP 4181
SLQ+ +VG+ YSD L K ++ K + L+ G + AL+ E +G +G + ++V+ G +P AE L++ GI V IG +KD +L ++SS+ + P + +A L T A + P ++ DI +VD S+ + L + F + ++ ++ + LV +++ + + + +L ++ +L + G T AL + + S+ ++VVL+T+G S + VQ + A L+ GV + +G+ + D +EL+ ++ P F ++F ++L ++ C T ++ + D++F++D S + + +++ + + L + + +I + YSD+ TEF + T+ ++ I +L R G G L+R ++ V + VV+ T +D + E A+IL+ GV I+ VGL P +L + + ++N + + ++ D K +EC + D++ ++ +S NFQ + F+ V +L++ D TR+ + ++ + L +D A + + ++++ F G G A+ +F ++ V ++ VV+ ESP + V + G+ I + G K+L I + + + +++F L K + + C IP+ G D+ F++ S + F +M DFMK + K+ + + R +++++ I F Y + ++I+++ G GKAL+ +F++ R + + +I+I DG+ TD E A L+ G+ ++ IG+ + ++L+ +AS K F+ F +L+ + + ++C +++ C +++ D++F +D S SI P ++ DF+ER+V +I A+ ++G++ F+++ ++EF L++++ K M A+ + T G+AL +S+R P+V ++++TDGVS ++P
Sbjct: 61 SLQVGRDNIRVGLAQYSDTPTSEFLLSVYHRKGDVLKHIRGLQFKPGGNRMGQALQFILEHHFREGAGSRASQGVPQVAVVVSSGLTEDHIREP---AEALRRAGILVYAIG-----VKDASQAELREISSSPKDNFTFFVPNFPGLPGLAQKLRPELCSTLGKAAQYTERESPACSEASPADIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQSSLKSAVLDRIRNLPYSMGGTSTGSALEFIRANSLTEMSGSRAKDGVPQIVVLVTDGESSDEVQDV--ADQLKRDGVFVFVVGI-NIQDVQELQKIANEPFEEFLFTTENFSILQALSGTLLQALCSTVERQMKKSTKTYADVVFLIDTSQGTSQASFQWMQNFISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRF---LHRTFFQEAAGSRLLQGVPQYVVVITS---GKSEDEVGEVAQILRKRGVDIVSVGLQDFDRAELEGIGPVVLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVPVPADLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVII-------ESPSLDNVSTPASYLRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNK-LKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSD-KIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQSTD--PVAEAAQGLRDIGVNIYAIGVR-DANTTELEEIAS-KKMFFIYEFDSLKSIHQEVIRDIC-----SSENCKSQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKAL-NFTLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAP 1148 HSP 2 Score: 163.696 bits (413), Expect = 8.962e-40 Identity = 256/1063 (24.08%), Postives = 479/1063 (45.06%), Query Frame = 3
Query: 945 TSNYIEISTPDQNCNN-QQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI----------SKGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAK--LDIALIVDCS---SQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF-TH-IYDDILKKV-ESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN--------------GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL--RSNGNGGDLNTALNRAR-QVFKID-----YRADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNS------------PYNF------YENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQR-QDEC-TMKKLDVIVTIDKSTSLGPENFQIEL-DFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKA-KLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK-NFESSIEDMTTS-------TNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSS 3863
+ Y E +P C+ D++F++D+S+S+ L Q++ F ++V+ L + QVG++ YSD + + PL K+ + + L G T +AL EFI S+ ++G + V+LVTDG + EV+ A++LK+ G+ V V+G+ + +++ L K+++ E + E+ S + T + Q C + + S K D+ ++D S SQ +W+ F R I E+G +K + L ++++ E F TH ++++ + E L GG + + L R+F+ ++ S+ L VV++T+G S + V + A L++ GVDI ++GL D DR EL+ + L D + E + + + V+ + KP ++ D++F+V+ + + + + TT+ SLNI T+R+S++ YS++ EF LD + + + +L R+ AL+ R +VF + +R V +I V+ + + D++S A L+ GV I G S P+ + L+ + +K + ++ + + I ++ +Q Q+ C +++K D+ ID S S+ P +F IE+ DF+ +++ + DR R G+ ++ ++ + A + I+ I + G T G A+ R DV + L+V+TDG + P A + + G+ I ++GV + + EL +IAS F+ + F L+ + + I S+E C+ D+IF++ S + + F++M DFM+ + + + + ++ +L+F+ S +E+++ N SS DM + ++G GKAL F S R + +I+I DG ++ A L+ I +F IG+ L+ DK +NF +LQ I SE+C +++ C+ +DL +D+S+S
Sbjct: 217 AAQYTERESP--ACSEASPADIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQSSLKSAVLDRIRNLPYSMGGTSTGSAL-----EFIRANSLTEMSGSRAKDGVPQIVVLVTDGESSD-----EVQDVADQLKRDGVFVFVVGINIQDVQE-LQKIANEPFEEFLFTTENFSILQALSGT-LLQALCSTVERQMKKSTKTYADVVFLIDTSQGTSQASF-QWMQN--FISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSEDEVGEV--AQILRKRGVDIVSVGLQD-FDRAELEGIGPV---VLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVPVPADLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRG-VQQIAVVIIE---SPSLDNVSTPASYLRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDF-IEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGV-RDANTTELEEIASKKMFF-IYEFDSLKSIHQEVIRDICSSENCK--------SQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFS------SNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKALNFTLPFFDSSRGGRPRVHQYLIVITDG--VSQDNVAPPAKALRDRNIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESLQSLEKEILSEVC-----SSQGCN---IDLSVGVDTSTS 1222 HSP 3 Score: 148.288 bits (373), Expect = 4.812e-35 Identity = 218/1034 (21.08%), Postives = 437/1034 (42.26%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN---PFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQT------CS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN--RARQVFKID---YRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLS----------KNSPYNFY----ENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGN---TAIGWAIGNATDYLFTKGR---EDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS-------SGE------FYNVKTFTDLEKFNAKTISN------EACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED-YKNFESSIEDMTTSTNGVFIGKALTSARVLF----------KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS--PDK-ALFVDNFSNLQRYNAYISSELC-DVIKG---------------TNKEC-STKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFI----TRESCKAGK 4370
DI +V S Q V L+ ++G + R L +++ E + H D+LK + L+ G R+ +AL + + S+ ++ V++++GL+ + ++ + A L+ +G+ + IG+ D A + EL+ +S+ P F +F L +K C T T CS S DI+F+VD ST + + ++ + + + L++ ++ ++ YSD F L ++ K + I+ L + G +AL RA + ++ + V +IVVL TD +++ + A+ LK DGV + VG++ N P+ + EN + ++ +C +++ + T DV+ ID S +FQ +F++RI+ L++ +D+ +IG+ ++ + + +++ +M I+++ +G T G + T + G + VP+ +VV+T G +++GE QI + G+ I+SVG+ Q EL I GE +V F + +++ E C +P DL+F++ ++ F+++ F+ + + + TRV+++ ++ ++ F D Y++ + + T G+A A + F S + + ++II+ D T A+ L++ G+ ++ G + DL+ + + P K A+ +++F L + +LC +++ G T + C S ++ D+ F +D S SI P+ + DF++ +++ I + + GVV ++ +F L Q+ + +A+ I G T G+AL + N+ R +V + ++++TDG ST P E+ + I + ++L+ ++S F+ I +F +LK I + E+CK+ K
Sbjct: 34 DIVFLVHNSINPQHAHSVRNFLYI--LANSLQVGRDNIRVGLAQYSDTPTSEFLLSVYHRKGDVLKHIRGLQFKPGGNRMGQALQFILEHHFREGAGSRASQGVPQVAVVVSSGLTEDHIR--EPAEALRRAGILVYAIGVKD-ASQAELREISSSPKDNFTFFVPNFPGLPGLAQKLRPELCSTLGKAAQYTERESPACSEASPADIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQSSLKSAVLDRIRNLPYSMGGTSTGSALEFIRANSLTEMSGSRAKDGVPQIVVLVTDGESSDEVQDV--ADQLKRDGVFVFVVGINIQDVQELQKIANEPFEEFLFTTENFSILQALSGTLLQA------------LCSTVERQMKKSTKTYADVVFLIDTSQGTSQASFQWMQNFISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSEDEVGEVAQILRKR----GVDIVSVGL-QDFDRAELEGIGPVVLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVP-------VPADLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLE--FSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVIIESPSLDNVST--PASYLRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGVRDANTTELEEIASKKMFF-IYEFDSLKSIHQEVIRDICSSENCKSQK 1028 HSP 4 Score: 112.079 bits (279), Expect = 4.696e-24 Identity = 199/902 (22.06%), Postives = 379/902 (42.02%), Query Frame = 3
Query: 1002 DLIFVLDAS---SSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPI--------EDMSKMALFDT--------------TDIAQDA---CQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE--EEFTHIYDDILKKVESL--KHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKP---------------QTCSS--NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKV---LVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG-DLIFVMGSSANKTSERFKQ-----MSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTST 3485
D++F++D S S + W Q F + +I L++ Q+G+ YSD + + + E+ + +L + +G + LR F S+ +G + V+++T G EV A+ L+K G+ ++ +GL Q + E P+ ED + + D TD+A+DA C +P P D+ +V+ S + + + F + + + TR SLV ++ + +E + +L+ ++ L + G + AL+ + +L K S+ ++ V++ S++ V T A+Y L+ +GV I G ++ ++L+ + T+P + + F + + K K C + P + C S DI F++D S + ++ M ++ +I R ++ YSD+ ++F L + + + AI ++ G G AL++ VF+ R DVA+ +++ TD T D ++EA + L+ GV I +G+ +++ E +ASKK E + ++E I++ IC + + C +K D+I ID S S+ P++F+ DF+ R+V ++ D +IG+ F+S ++ DM + I + + T G A+ N T F R P+V L+V+TDG + +P A++ + I I ++GVG + L + + + F L+ + +S +C + G ++ +G + +SER +Q + + M+ L L+ + E +F Y+ G D F+S E + T
Sbjct: 430 DVVFLIDTSQGTSQASFQWMQN--FISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSEDEVG--EV-AQILRKRGVDIVSVGL-------QDFDRAELEGIGPVVLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVPVPA-------DLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVIIESPSLDNVST-PASY-LRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGV-RDANTTELEEIASKKMFFIYEFDSLKSIHQEVIRD-ICSS-----ENCKSQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKAL-NFTLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAPPAKALRDR--NIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESLQSLEKEILSE----------VCSSQGCNIDLSVGVDTSTSSERAQQELRRLLPELMQQLAFLSNISCEAPGQMEPRF---RYVVPG-SSDQPVFDSGFEKYSDET 1283 HSP 5 Score: 67.781 bits (164), Expect = 1.533e-10 Identity = 100/449 (22.27%), Postives = 203/449 (45.21%), Query Frame = 3
Query: 918 IPFETKRTTTSNYIEISTPDQNCNN-QQLDLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT-HIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSL 2222
IP T+++T + C + ++ D+ F++D S S+ E+ F VIK I + GV+ YSD + L + + + ++ + G T AL + F + R + +I++TDG P + A+ L+ +G+ + IG ++D +L +++S + ++ E S ++ ++ +D C K S K DI ++D S + ++ F +R + Q +G ++ + L+ F++ +EEF + Y D+ + + S++ + T KALN + F+ S+ + + ++++T+G+S + V A L++ + I IG+G+ + L+I + F ++F+ +SL ++ + C + Q C N D+ VD ST + + +R L+ +Q L
Sbjct: 830 IPESTRQST----------QEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQSTDPVAE---AAQGLRDIGVNIYAIG-----VRDANTTELEEIASKKMFFIYEFDSLKSIHQ--EVIRDICSSENCK---SQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNP--QEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKALNF-TLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAP--PAKALRDRNIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESLQSLEKEILSEVCSS------QGC--NIDLSVGVDTSTSSERAQQE-LRRLLPELMQQL 1241 HSP 6 Score: 63.929 bits (154), Expect = 1.854e-9 Identity = 50/180 (27.78%), Postives = 91/180 (50.56%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEID--RILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
DI ++D S SI +F + + + F I P+ R + Y D + ++F T++ S L+ A+ ++VGG ++ G+AL+ +VP + T+ ID R L++++DG +TD V + A +++ GV IY AIG L + K + EF +++ + ++ IC
Sbjct: 849 DIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTT-GKALSK----MVPVFQNTARIDVARYLIVITDGQSTD--PVAEAAQGLRDIGVNIY--AIGVRDANTTELEEIASKKMFFIYEFDSLKSIHQEVIRDIC 1019
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Match: Col6a3 (collagen, type VI, alpha 3 [Source:MGI Symbol;Acc:MGI:88461]) HSP 1 Score: 166.392 bits (420), Expect = 9.594e-41 Identity = 278/1155 (24.07%), Postives = 502/1155 (43.46%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS--STETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSA-------KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--THIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKS------------KNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXX-------XXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLR-SNGNGGDLNTALNRA-RQVFKIDYRA----DVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNT-----AIGWAIGN-ATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQ-ISEKE----LNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQ------KPTICKAAG-------DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNG---VFIGKALTSA-RVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYI--------SSELCDVIKGTNKECSTKQ---LDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
D++F++D SSSL ++ F VI+ L+I QV V Y+D ++ L S NK + V K+ L G+ F+ N L S + EG K ++L+T G +P A++LK+ I + +G + +D+L +++ S+ + P E F + + P R + K DI ++D S V + + F + ++G + R LV F++ V E ++L + L+ GG LN S Y+ + ++ +L++LL G S + +QAA L SGV +G ++AD+ EL+ ++ P+ +L DF+ SL L V + P +P+ S DI+F+ D S + + + +R + ++ L++ +R++I +SD+ E R E TK +I +K+++ G +L AL+ A R +F + +V + +VL + D+++ A LK GV + F+ +KN+ + E + + I + + + A +Q + +K DV+ ID S + F + DFV R+V++LD+ DR R+ + ++ E YLN S+ + + I ++ + G T A+ + + N T ++ E VP++L+VLT A G+ + +V K GG I +G+G ISE + + D A + + TF +L + IS Q+ + KP + + G D++F++ S N E F+ + ++ + + + + TRVA+++F+ + ++ + E G V+IG AL + +F+ S + + +++I G+ D E + A LK+ G+ TI T + +L ++ SP+ V F L R + S ++ ++ T S + D++F +DSS ++ PD DFV RIVR ++I + ++GVV F+ D EF L + +++ A++R+ F G+ + G AL L +R E VP+ +VL G S
Sbjct: 444 DIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAG--HRAAEGVPKLLVLITGGKSLDEVSQP---AQELKRGSIMALAVG-SKAADEDELKEIAFDSSLVFIPAE-------FRPAPLQNMLPSLMAPLRTLTGTTEVHVNKRDIIFLLDGSDNVGKNNFPYVRDFVTNLVNSLDVGSDNIRVGLVQFSDTPVTEFSLDTYQTKSELLAHLRRLQLKGG-----SGLNAGSALSYIHANHFTEAGGSRTREHVPQLLLLLMAGPSED--AYLQAANALVRSGVLTFCVGT-NRADKAELEHIAFNPSLVYLMDDFRSLPSLPQQLIQPLTTYVSGGVQEVPLSQPE---SKRDILFLFDGSVNVLGQ-FPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLL---VAGRSSDAVAGPASSLKQRGV-VPFIFQAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKA-EQGSGPVSGEK-DVVFLIDGSEGVR-SGFPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLN----SHMDQQGVISAIRRLTLLGGPTPNTGAALEFVLRNILTSSTGSRIAEGVPQLLIVLT--AEPSGDDVRGPSVVLKQGGAVPIGIGIGNADISEMQTISFIPDFAVA-----IPTFRELGTIQ-QVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPE-FQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSDD--EVDDSAVELKQFGVAPLTIAR--HTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKTHKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKS 1549 HSP 2 Score: 112.079 bits (279), Expect = 3.770e-24 Identity = 251/1137 (22.08%), Postives = 485/1137 (42.66%), Query Frame = 3
Query: 1002 DLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT---------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVI-KDQLNKMSSTET-------YYPIEDMSKMALFDTTDIAQDACQIPKPKRICSA----KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDI--LKKVESLKHVGGFT--RIDKALN--LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKA-DREELKILSTFPNP--FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGG-DLNTAL-----NRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXX-----XXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTS-LGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDSGFKEDYKNFESSIEDMT-----TSTNGVFIGKALTSARVLFKSDTRKNATKTV---IIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYIS-----------SELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALREC-----KNELLNSTRKEVPKVIVLVTDGVS 4163
D+IF++D S + +D F NV++ L + + QVGV+ YS+ L S +K + V L L+ G + + N R+G S+ EG + ++L++ G +S ++ V A K V GLG + +L +++ ++ + D+ + L +AQ + P + K DI +VD SS + + F R I++ E+G + + S+ +A+ E +T+ D I ++K+ +L +T +D N S + + ++ +L+VL+T G S++ V Q A +L+ I + +G KA D +ELK ++ F + F+ +F+ ++ T + + DIIF++D S + + +R + + SL++ R+ ++ +SD TEF LD TK ++ +++L+ G G + +AL N + R V ++++L ++ L A L GV VG ++ E++A S + + + + + + ++ K D++ D S + LG F DF+ RI++ LD++ D TR+ I F+ +V L + A++ + K+ G +G+A+ A +F + ++V + LV+L G + + S++K + G + I + + EL I S F ++ + ++ +S + P D++F++ S S F + DF++ + + V + RVA+++++ Y++S D + S+I +T T G + L R + S T + V +I++ E + ++ + +LK+ G IG+ G S++++++ PD A+ + F L IS S L ++ + + D++F +D S + GP++ Q +ERIV +DI + T+V V+ F+ D++ EF L+ K + +AV+R+ G+ IG AL + L + + VP+ +VL++ G S
Sbjct: 241 DIIFLIDGSQNTGNANFDVIRDFLVNVLERLSVGNQQVQVGVVQYSEEPITMFSLNSYPSKAAVLDAVKGLSLVGGESANIGQALDFVVENHFTRAGG---SRVEEGVPQVLVLISAGP-SSDEIRDSVVALKQAS----VFSFGLGAQAASRAELQHIATDDSLVFTVPEFRSFGDLQEQILPYLVGVAQRHIVLQPPAIVTQVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLDEVS--QPAQELKRG--SIMALAVGSKAADEDELKEIA-FDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTTEVHVNKRDIIFLLDGSDNVGKNNFPYVRDFVTNLVNSLDVGSDNIRVGLVQFSDTPVTEFSLDTYQTKSELLAHLRRLQLKGGSGLNAGSALSYIHANHFTEAGGSRTREHVPQLLLLLMAG--PSEDAYLQAANALVRSGVLTFCVGTNRADKAEL-EHIAFNPSLVYLMDDFRSLPSLPQQLIQPLTTYVSGGVQEVPLSQPESKRDILFLFDGSVNVLG--QFPAVRDFLYRIIEELDVKPDGTRVAIAQFSDDVRLESRFSEHQTKAEILNLVKKMKLKTGKALNLGYALDYALRNIFVRSAGSRIEDNVQQFLVLLVAGRSSDAVAGPASSLKQR-GVVPFIF--QAKNANPSELEQIVLSPAFILAAESLPKIGDLQSQIVSLLKAEQGSGP--VSGEKDVVFLIDGSEGVRSG-FPLLKDFVQRVVESLDVGPDRVRVALVQYSDRTRPEFYLNSHM--DQQGVISAIRRLTLLGGPTPNTGAALEFVL---RNILTSSTGSRIAEGVPQLLIVLTAEPSG-DDVRGPSVVLKQGGAVPIGIGI-GNADISEMQTISFIPDFAVAIPTFRELGTIQQVISERVIQLNREELSSLKPILTPSTGAGVGSKKDVVFLIDGSRNAGPEF-QYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKS 1345 HSP 3 Score: 105.145 bits (261), Expect = 5.950e-22 Identity = 115/488 (23.57%), Postives = 224/488 (45.90%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---------TKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISN----EACQIPQ-------KPTICKA-AGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMT----TSTNGVFIGKALT-SARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYI---------SSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAV 4034
K DV+ ID S + GPE FQ + RIV+ LD+ D TR+ + F+ + + P++++F ++Q + ++ G+ IGNA +Y+ ++ E VP+ LV+++ G +D + SAV+ K G+ +++ + ++EL I+ S E+ Y+V TF +L + K ++ + QI Q P++ ++ A D++F++ SS + + DF+ + + + R+ +++F+ + + K +K+ S +E + + + G+AL AR LF S + +++ + G+ D + A ++ GI IG +DL+++ + + +F V F L + + + V + K+ D++F LD S + D +Q L F IV + + + +VG+V +N+D EF L F+ K +I A+
Sbjct: 1228 KKDVVFLIDGSRNAGPE-FQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVY---IGNALEYVLKNIFQRPLGSRIEEGVPQFLVLISSGKSD--DEVDDSAVELKQFGVAPLTIA--RHTDQEELVKISLSPEYVYSVSTFRELPRLEQKLLTPITTLTSQQIHQILASTRYPPSVVESDAADIVFLIDSSDAVKPDGIAHIRDFVSRIVRRLNIGPSKVRIGVVQFSNDVFPEFYLKT-HKSQSSVLEAIRRLRFKGGSPLNTGRALEFVARNLFVKSAGSRIEDGVPQHLVLFLGGKSQD--DVARHAQVISSSGIVSLGIG-DRNIDRTDLQTITNDPRLVFTVREFRELPNIEERVMLSFGPSGATPQPPGVDLPSPSRPEKKKADIVFLLDGSINFRRDSFQEVLRFASEIVDTVYEDGDSIRVGLVQYNSDPTDEFFLRDFSTKRQIIDAI 1703 HSP 4 Score: 98.2117 bits (243), Expect = 6.092e-20 Identity = 116/557 (20.83%), Postives = 238/557 (42.73%), Query Frame = 3
Query: 2664 KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAI-----GNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS---SGEFYNVKTFTDLEKFNAKTISNEACQI-PQ--------KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKS-LTKLTV---------------------VDKENTRVAILKFNRGNYIDSGFKEDY-KNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKT-SESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKG---------TNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRI-IFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVS 4163
+Q D D++ +D S S G + F + +F++ +V++L + + + N + +++ ++ I + +I G+ G + + T+ ++ + VP+V++VLTDG ++ G + + + + K + + +VGV + E+ L ++AS S +N++ T L +S + P+ K + + D+IF++ S N + F + DF+ + L +L+V ++ ++ A+L +G + G + + + +E+ T G + + + VL + + EI D ++A+ VF+ GL + S ++L+ +A+ D +F V F + I L V + + + D++F +D SSS+GP + DFV R+++ ++I + +V V + + EF L+ +T K I+AV+++ + A G +L +N L S+ VPK++VL+T G S
Sbjct: 26 QQHGDVKNGAAADIVFLVDSSWSAGKDRFLLVQEFLSDVVESLAVGDNDFHFALVRLNGNPHTEFLLNTYHSKQEVLSHIVNMSYIGGSNQTGKGLEYVIHSHLTEASGSRAADGVPQVIIVLTDGQSEDGFA--LPSAELKSADVNVFAVGV-EGADERALGEVASEPLSMHVFNLENVTSLHGLVGNLVSCIHSSVNPERAGDKESLKDITAQDSADIIFLIDGSQNTGNANFDVIRDFLVNVLERLSVGNQQVQVGVVQYSEEPITMFSLNSYPSKAAVLDAVKGLSLVGGESANIGQALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSSD-----------EIRDSVVALKQAS--------VFSFGLGAQAASRAELQHIATDDSLVFTVPEFRSFGDLQEQILPYLVGVAQRHIVLQPPAIVTQVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSVAQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKS 560 HSP 5 Score: 97.4413 bits (241), Expect = 1.259e-19 Identity = 122/536 (22.76%), Postives = 235/536 (43.84%), Query Frame = 3
Query: 2673 QDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEK-ELNDIASSGEFY----NVKTFTDLEKFNAKTISNEACQ--IPQKPTICKAA-----GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTST------NG--VFIGKALTSARV-LFKSDTRKNAT----KTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDV--IKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALRECKNELL-----NSTRKEVPKVIVLVTDGVS 4163
+D D+I ID S + G NF + DF+ +++ L + + ++G+ ++ E ++S A + + + + G +A IG A+ + FT+ E VP+VLV+++ G + S +I L + S G+G Q + + EL IA+ ++F DL++ + A + + Q P I D++F++ S++ F + DF+ + + + ++ +V++ Y D+ E Y N ++ D T+ NG ++ G +L R LF S A K +++I G+ D E + A LK+ I +G + E +LK +A +F+ + S + + + GT E + D++F LD S ++G + + DFV +V +D+ ++ +VG+V F+ EF LD + K +++ ++R+ G+ + G AL + TR+ VP++++L+ G S
Sbjct: 232 KDITAQDSADIIFLIDGSQNTGNANFDVIRDFLVNVLERLSVGNQQVQVGVVQYSEEPITMFSLNSYPSKAAVLDAVKGLSLVGGESANIGQALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPS----SDEIRDSVVALKQASVFSFGLGAQAASRAELQHIATDDSLVFTVPEFRSFGDLQEQILPYLVGVAQRHIVLQPPAIVTQVMEVNKRDIVFLVDGSSSLGPSNFNAIRDFVTRVIQRLEIGQDLVQVSV-----AQYADTVKPEFYLNSYTNKRDAITAVRKMRALNGSALYTGSSLDFVRNNLFTSSAGHRAAEGVPKLLVLITGGKSLD--EVSQPAQELKRGSIMALAVG-SKAADEDELKEIAFDSSLVFIPAEFRPAPLQNMLPSLMAPLRTLTGTT-EVHVNKRDIIFLLDGSDNVGKNNFPYVRDFVTNLVNSLDVGSDNIRVGLVQFSDTPVTEFSLDTYQTKSELLAHLRRLQLKGGSGLNAGSALSYIHANHFTEAGGSRTREHVPQLLLLLMAGPS 754 HSP 6 Score: 51.9878 bits (123), Expect = 7.914e-6 Identity = 42/148 (28.38%), Postives = 70/148 (47.30%), Query Frame = 3
Query: 135 IGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSE-----IDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
+G +D+ ++D S + EFQ I + + + I + TRV+ + + + K EF H S + AVR R GG +G AL L +I RP + + L+++S G + DE V D A ++++ GV T+A
Sbjct: 1225 VGSKKDVVFLIDGSRN-AGPEFQYIRTLIERIVEYLDIGFDTTRVAVIQFSEDSKMEFPLNAHFSKDEVQNAVRRLRPKGGSQVYIGNALEYVLKNIF--QRPLGSRIEEGVPQFLVLISSGKSDDE--VDDSAVELKQFGVAPLTIA 1367
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|Q8C6K9|CO6A6_MOUSE (Collagen alpha-6(VI) chain OS=Mus musculus OX=10090 GN=Col6a6 PE=1 SV=2) HSP 1 Score: 226.483 bits (576), Expect = 3.300e-58 Identity = 271/1132 (23.94%), Postives = 506/1132 (44.70%), Query Frame = 3
Query: 975 DQNCNNQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSK-LELLEGATFISNALRRSGEEFISKGREGAKK----TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF---------DTT--------DIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFL-------------RQDFXXXXXXXXXXXXXXCKTTPAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN---RARQVFKIDYRADVAKIVVLFTDKLPTNQKDS-LSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-KG-REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKELNDIASSGE--FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDS-GFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECK----NELLNSTRKEVPKVIVLVTDGVS 4163
+Q+ + D++F++D+S L L + F + +I SL I+ +V + YSD L LG+ +N+ + + K + G+ I NAL+ + + S G K +++V A++ + E A+ L++ G+++I +G+ ++ L M++++ ++ + +++F D T DI +ACQ P + D+ ++D + + + F I ++ N R LV+++NE V + + ++L++++ L G AL + + + S++ + VL+T+ S + V +AA +L+ GV I T+G+ + A+ +EL+ +++ P Q F ++ C + DI ++D S + +++ + + NI+ R+ + Y+D EF + + K D+ AI+ +R G + ALN + Q K + + V +V+ T+ + +DS L A L+ + +++ +G+ + + E +K + + + + Q+ IC ++ C K D++ +D S S+GPENF F+ +V + DR +IG+ F+ E +++ +D+ I+++ I T G A+ + Y KG R +V K L+++TDG A DI P I+ K G+ I SVGV G +++ L +I+ E FY V+ F L+ + C P++ D++FV+ SS + + + M DFM L K V K R LK+ Y+D G K + + + M +T + +AL + +F S K + +I+I DGE D + A L+ GI V +G+ G S L S DK FV+ F L+ + +S+ +C+ K +C +++DL+F +D S+SI PD +Q F+ +V+D D++ N ++GV F+ R EF L FT + + + ++ I G T IG+ALR+ K ++ + P+V++++TDG S
Sbjct: 17 NQDSGPEYADVVFLVDSSDHLGLKSFPLVKTFIHKMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFGFIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDV--EEAAKALREDGVKIISVGV-QKASEENLKAMATSQFHFNLRTARDLSVFAPNMTEIIKDVTQYREGMADDIIVEACQGP-------SVADVVFLLDMAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNV--TKAAVNLRREGVTIFTMGI-EGANPDELEKIASHPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGM---SRDSVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEIC-----AEEACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITDGEAQDIVRDPAIALRKE---GVIIYSVGVFGSNVTQ--LEEISGKPEMVFY-VENFDILQHIEDDLVLG-ICS-PREECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNT---YTAEALAFSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSK---VDCEIEKVDLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRS 1113 HSP 2 Score: 179.104 bits (453), Expect = 1.190e-43 Identity = 237/1012 (23.42%), Postives = 448/1012 (44.27%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQE-----LAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGR-----EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIE-------DMSKMALFDTTDIAQDACQIPKP-----------KRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALN----LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID--YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF---NAKTISNEA---CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSS 3860
D++F+LD +A+ QE AF I +L I +VG++ YS+ + L + NKTE+ + + L G + ALR++ +E S R +G + +LVT A K V L++ G+ + +G+ D+L K++S +P E + S++A + T + + QI K C + + DI L++D S Q ++ F + F + +K R V +A+ +E E + + D+ K +E+++ +GG T ALN L R+ + +V+LTNG+S + V + A+ L+E + + IG+ + + +I + +F +++ + V+ C + C DI+F+VD S E + ++ M + I +I ++ +S E EF+L+ ++ DI AI ++ G +AL Q F D R +V K ++L TD +D + + I L+ +GV I VG+ ++ E + +EN + + ++ + IC ++EC ++ LDV+ ID S S+ + + I DF+ +V+ D+ K++ R G + + + +D ++ + + GNT A+ +D++FT+ R + VP+VL+V+TDG + E +A + GI +++VG+ S + L SS ++Y V+TF L+ + ++ N + C+I + DL+F+M S + + F++M F+ S+ + V R+ + +F+ R ++ F + + + IE + IG AL + F+ D +R NA + ++++ DG D E + A L+ G+ ++++G+ + ++ + +K L V NF L++ I +C G C +D++ D SS
Sbjct: 228 DVVFLLD----MAINGSQEDLDHLKAFLGESISALDIKENCMRVGLVTYSNETRVISSLSTGNNKTEVLQRIQDLSPQVGQAYTGAALRKTRKEIFSAQRGSRKNQGVPQIAVLVTHRASEDNVTKAAV---NLRREGVTIFTMGI-EGANPDELEKIAS----HPAEQFTSKLGNFSELATHNQTFLKKLRNQITHTVSVFSERTETLKSACVDTEEADIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNTGAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRDSV--LGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICAE------EACRDMKADIMFLVDSSGSIGPENFSKMKMFMKNLVSKSQIGADRVQIGVVQFSHENKEEFQLNTFMSQSDIANAIDRMTHIGETTLTGSALTFVSQYFSPDKGARPNVRKFLILITD---GEAQDIVRDPAIALRKEGVIIYSVGVFGSNVTQLEEISGKPEMVFYVENFDILQHIEDDLVLGICSP----REECKRIEVLDVVFVIDSSGSIDYQEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDELGTKLEVVSVLQNDHPMGGNTYTAEALA-FSDHMFTEARGSRLHKGVPQVLIVITDGESHDAEKLNTTAKALRDKGILVLAVGIAGANSWELLAMAGSSDKYYFVETFGGLKGIFSDVSASVCNSSKVDCEIEKV--------DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGE-REISTQIEGIQQIFGYTHIGDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQD--EVAQAAEELRHKGVDIYSVGIGDVDDQELVQITGTAEKKLTVHNFDELKKVKKRIVRNIC--TSGGESNCF---VDVVVGFDISS 1195 HSP 3 Score: 66.2402 bits (160), Expect = 2.702e-9 Identity = 48/180 (26.67%), Postives = 88/180 (48.89%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRP-TSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICV 686
DI +++D S S +F +++ + F I P+ RV + Y DT EF +K+++ P L A+ R++GG+++ G ALN TL + + S++ L++L++G + D SV A K++E+ + ++ + + L +K V EF +R +V IC
Sbjct: 435 DIYLLIDGSGSTQPTDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADTWDLEFEISKYSNKPDLGKAIENIRQMGGNTNT-GAALNFTLKLLQRAKKERGSKVPCHLVVLTNGMSRD--SVLGPAHKLREENIRVHAIGVKEANQTQLREIAGEEKRVYYVHEFDALRNIRNQVVQEICA 611 HSP 4 Score: 57.7658 bits (138), Expect = 1.122e-6 Identity = 40/142 (28.17%), Postives = 72/142 (50.70%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM--RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
D+ ++D S+SI +FQ + + F + N R+ + D+ ++EFL T ++ + +++ G + + G AL Y P+M R + ++LL+L+DG + DE V A +++ KGV IY+V IG
Sbjct: 999 DLVFLMDGSNSIHPDDFQKMKGFLVSVVQDFDVSLNRVRIGVAQFSDSYRSEFLLGTFTGEREISTQIEGIQQIFGYTHI-GDALRKVKYYFQPDMGSRINAGTPQVLLVLTDGRSQDE--VAQAAEELRHKGVDIYSVGIG 1137
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|A2AX52|CO6A4_MOUSE (Collagen alpha-4(VI) chain OS=Mus musculus OX=10090 GN=Col6a4 PE=1 SV=2) HSP 1 Score: 171.785 bits (434), Expect = 1.936e-41 Identity = 238/1124 (21.17%), Postives = 486/1124 (43.24%), Query Frame = 3
Query: 1086 SLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-----REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTET-----YYP-IEDMSKMA------LFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANE--AVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL-------RSNGNGGDLNTALNRA--RQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLS--------KNSPYNFYENL-AXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMK-KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFI--EGNTAIGWAIGNATDYLF-----TKGREDVPKVLVVLTDGANDIGESPQISAVKA-----KLGGIKIISVGVGQQISEKELNDIASSGEFYN---VKTFTDLEKFNAKTISNEACQ------------IPQKPTICKAAG-------DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKV---IVLVTDGVSTGGLSP 4181
SLQ+ +VG+ YSD L K ++ K + L+ G + AL+ E +G +G + ++V+ G +P AE L++ GI V IG +KD +L ++SS+ + P + +A L T A + P ++ DI +VD S+ + L + F + ++ ++ + LV +++ + + + +L ++ +L + G T AL + + S+ ++VVL+T+G S + VQ + A L+ GV + +G+ + D +EL+ ++ P F ++F ++L ++ C T ++ + D++F++D S + + +++ + + L + + +I + YSD+ TEF + T+ ++ I +L R G G L+R ++ V + VV+ T +D + E A+IL+ GV I+ VGL P +L + + ++N + + ++ D K +EC + D++ ++ +S NFQ + F+ V +L++ D TR+ + ++ + L +D A + + ++++ F G G A+ +F ++ V ++ VV+ ESP + V + G+ I + G K+L I + + + +++F L K + + C IP+ G D+ F++ S + F +M DFMK + K+ + + R +++++ I F Y + ++I+++ G GKAL+ +F++ R + + +I+I DG+ TD E A L+ G+ ++ IG+ + ++L+ +AS K F+ F +L+ + + ++C +++ C +++ D++F +D S SI P ++ DF+ER+V +I A+ ++G++ F+++ ++EF L++++ K M A+ + T G+AL +S+R P+V ++++TDGVS ++P
Sbjct: 61 SLQVGRDNIRVGLAQYSDTPTSEFLLSVYHRKGDVLKHIRGLQFKPGGNRMGQALQFILEHHFREGAGSRASQGVPQVAVVVSSGLTEDHIREP---AEALRRAGILVYAIG-----VKDASQAELREISSSPKDNFTFFVPNFPGLPGLAQKLRPELCSTLGKAAQYTERESPACSEASPADIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQSSLKSAVLDRIRNLPYSMGGTSTGSALEFIRANSLTEMSGSRAKDGVPQIVVLVTDGESSDEVQDV--ADQLKRDGVFVFVVGI-NIQDVQELQKIANEPFEEFLFTTENFSILQALSGTLLQALCSTVERQMKKSTKTYADVVFLIDTSQGTSQASFQWMQNFISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRF---LHRTFFQEAAGSRLLQGVPQYVVVITS---GKSEDEVGEVAQILRKRGVDIVSVGLQDFDRAELEGIGPVVLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVPVPADLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVII-------ESPSLDNVSTPASYLRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNK-LKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSD-KIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQSTD--PVAEAAQGLRDIGVNIYAIGVR-DANTTELEEIAS-KKMFFIYEFDSLKSIHQEVIRDIC-----SSENCKSQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKAL-NFTLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAP 1148 HSP 2 Score: 163.696 bits (413), Expect = 6.269e-39 Identity = 256/1063 (24.08%), Postives = 479/1063 (45.06%), Query Frame = 3
Query: 945 TSNYIEISTPDQNCNN-QQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI----------SKGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAK--LDIALIVDCS---SQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF-TH-IYDDILKKV-ESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSN--------------GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL--RSNGNGGDLNTALNRAR-QVFKID-----YRADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNS------------PYNF------YENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQR-QDEC-TMKKLDVIVTIDKSTSLGPENFQIEL-DFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKA-KLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK-NFESSIEDMTTS-------TNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSS 3863
+ Y E +P C+ D++F++D+S+S+ L Q++ F ++V+ L + QVG++ YSD + + PL K+ + + L G T +AL EFI S+ ++G + V+LVTDG + EV+ A++LK+ G+ V V+G+ + +++ L K+++ E + E+ S + T + Q C + + S K D+ ++D S SQ +W+ F R I E+G +K + L ++++ E F TH ++++ + E L GG + + L R+F+ ++ S+ L VV++T+G S + V + A L++ GVDI ++GL D DR EL+ + L D + E + + + V+ + KP ++ D++F+V+ + + + + TT+ SLNI T+R+S++ YS++ EF LD + + + +L R+ AL+ R +VF + +R V +I V+ + + D++S A L+ GV I G S P+ + L+ + +K + ++ + + I ++ +Q Q+ C +++K D+ ID S S+ P +F IE+ DF+ +++ + DR R G+ ++ ++ + A + I+ I + G T G A+ R DV + L+V+TDG + P A + + G+ I ++GV + + EL +IAS F+ + F L+ + + I S+E C+ D+IF++ S + + F++M DFM+ + + + + ++ +L+F+ S +E+++ N SS DM + ++G GKAL F S R + +I+I DG ++ A L+ I +F IG+ L+ DK +NF +LQ I SE+C +++ C+ +DL +D+S+S
Sbjct: 217 AAQYTERESP--ACSEASPADIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQSSLKSAVLDRIRNLPYSMGGTSTGSAL-----EFIRANSLTEMSGSRAKDGVPQIVVLVTDGESSD-----EVQDVADQLKRDGVFVFVVGINIQDVQE-LQKIANEPFEEFLFTTENFSILQALSGT-LLQALCSTVERQMKKSTKTYADVVFLIDTSQGTSQASF-QWMQN--FISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSEDEVGEV--AQILRKRGVDIVSVGLQD-FDRAELEGIGPV---VLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVPVPADLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRG-VQQIAVVIIE---SPSLDNVSTPASYLRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDF-IEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGV-RDANTTELEEIASKKMFF-IYEFDSLKSIHQEVIRDICSSENCK--------SQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFS------SNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKALNFTLPFFDSSRGGRPRVHQYLIVITDG--VSQDNVAPPAKALRDRNIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESLQSLEKEILSEVC-----SSQGCN---IDLSVGVDTSTS 1222 HSP 3 Score: 148.288 bits (373), Expect = 3.366e-34 Identity = 218/1034 (21.08%), Postives = 437/1034 (42.26%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN---PFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQT------CS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN--RARQVFKID---YRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLS----------KNSPYNFY----ENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGN---TAIGWAIGNATDYLFTKGR---EDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS-------SGE------FYNVKTFTDLEKFNAKTISN------EACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED-YKNFESSIEDMTTSTNGVFIGKALTSARVLF----------KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS--PDK-ALFVDNFSNLQRYNAYISSELC-DVIKG---------------TNKEC-STKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFI----TRESCKAGK 4370
DI +V S Q V L+ ++G + R L +++ E + H D+LK + L+ G R+ +AL + + S+ ++ V++++GL+ + ++ + A L+ +G+ + IG+ D A + EL+ +S+ P F +F L +K C T T CS S DI+F+VD ST + + ++ + + + L++ ++ ++ YSD F L ++ K + I+ L + G +AL RA + ++ + V +IVVL TD +++ + A+ LK DGV + VG++ N P+ + EN + ++ +C +++ + T DV+ ID S +FQ +F++RI+ L++ +D+ +IG+ ++ + + +++ +M I+++ +G T G + T + G + VP+ +VV+T G +++GE QI + G+ I+SVG+ Q EL I GE +V F + +++ E C +P DL+F++ ++ F+++ F+ + + + TRV+++ ++ ++ F D Y++ + + T G+A A + F S + + ++II+ D T A+ L++ G+ ++ G + DL+ + + P K A+ +++F L + +LC +++ G T + C S ++ D+ F +D S SI P+ + DF++ +++ I + + GVV ++ +F L Q+ + +A+ I G T G+AL + N+ R +V + ++++TDG ST P E+ + I + ++L+ ++S F+ I +F +LK I + E+CK+ K
Sbjct: 34 DIVFLVHNSINPQHAHSVRNFLYI--LANSLQVGRDNIRVGLAQYSDTPTSEFLLSVYHRKGDVLKHIRGLQFKPGGNRMGQALQFILEHHFREGAGSRASQGVPQVAVVVSSGLTEDHIR--EPAEALRRAGILVYAIGVKD-ASQAELREISSSPKDNFTFFVPNFPGLPGLAQKLRPELCSTLGKAAQYTERESPACSEASPADIVFLVDSSTSIGLQNFQKVKHFLHSVVSGLDVRSDQVQVGLVQYSDNIYPAFPLKQSSLKSAVLDRIRNLPYSMGGTSTGSALEFIRANSLTEMSGSRAKDGVPQIVVLVTDGESSDEVQDV--ADQLKRDGVFVFVVGINIQDVQELQKIANEPFEEFLFTTENFSILQALSGTLLQA------------LCSTVERQMKKSTKTYADVVFLIDTSQGTSQASFQWMQNFISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSEDEVGEVAQILRKR----GVDIVSVGL-QDFDRAELEGIGPVVLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVP-------VPADLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLE--FSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVIIESPSLDNVST--PASYLRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGVRDANTTELEEIASKKMFF-IYEFDSLKSIHQEVIRDICSSENCKSQK 1028 HSP 4 Score: 112.079 bits (279), Expect = 3.285e-23 Identity = 199/902 (22.06%), Postives = 379/902 (42.02%), Query Frame = 3
Query: 1002 DLIFVLDAS---SSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPI--------EDMSKMALFDT--------------TDIAQDA---CQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE--EEFTHIYDDILKKVESL--KHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKP---------------QTCSS--NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKV---LVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG-DLIFVMGSSANKTSERFKQ-----MSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTST 3485
D++F++D S S + W Q F + +I L++ Q+G+ YSD + + + E+ + +L + +G + LR F S+ +G + V+++T G EV A+ L+K G+ ++ +GL Q + E P+ ED + + D TD+A+DA C +P P D+ +V+ S + + + F + + + TR SLV ++ + +E + +L+ ++ L + G + AL+ + +L K S+ ++ V++ S++ V T A+Y L+ +GV I G ++ ++L+ + T+P + + F + + K K C + P + C S DI F++D S + ++ M ++ +I R ++ YSD+ ++F L + + + AI ++ G G AL++ VF+ R DVA+ +++ TD T D ++EA + L+ GV I +G+ +++ E +ASKK E + ++E I++ IC + + C +K D+I ID S S+ P++F+ DF+ R+V ++ D +IG+ F+S ++ DM + I + + T G A+ N T F R P+V L+V+TDG + +P A++ + I I ++GVG + L + + + F L+ + +S +C + G ++ +G + +SER +Q + + M+ L L+ + E +F Y+ G D F+S E + T
Sbjct: 430 DVVFLIDTSQGTSQASFQWMQN--FISRIIGILEVGQDKYQIGLAQYSDQGHTEFLFNTHKTRNEMVAHIHELLVFQGGSRKTGQGLRFLHRTFFQEAAGSRLLQGVPQYVVVITSGKSEDEVG--EV-AQILRKRGVDIVSVGL-------QDFDRAELEGIGPVVLVSDLQGEDRIRQLMLDVNMFIQGSPKPPRVMTDVAKDAVEECLVPVPA-------DLVFLVEDFSSARQPNFQRVVHFLTTTVHSLNIHPDTTRVSLVFYSEKPRLEFSLDMYQSAAQVLRHLDRLTFRARRGRAKAGAALDFLRKEVFLPEKGSRPHRGVQQIAVVIIESPSLDNVST-PASY-LRRAGVTIYAAGTQPASESKDLEKIVTYPPWKHAIRLESFLQLSVVGNKLKKKLCPEMLSGMPPLMSFIPESTRQSTQEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQST---DPVAEAAQGLRDIGVNIYAIGV-RDANTTELEEIASKKMFFIYEFDSLKSIHQEVIRD-ICSS-----ENCKSQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNPQEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKAL-NFTLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAPPAKALRDR--NIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESLQSLEKEILSE----------VCSSQGCNIDLSVGVDTSTSSERAQQELRRLLPELMQQLAFLSNISCEAPGQMEPRF---RYVVPG-SSDQPVFDSGFEKYSDET 1283 HSP 5 Score: 67.781 bits (164), Expect = 1.073e-9 Identity = 100/449 (22.27%), Postives = 203/449 (45.21%), Query Frame = 3
Query: 918 IPFETKRTTTSNYIEISTPDQNCNN-QQLDLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT-HIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSL 2222
IP T+++T + C + ++ D+ F++D S S+ E+ F VIK I + GV+ YSD + L + + + ++ + G T AL + F + R + +I++TDG P + A+ L+ +G+ + IG ++D +L +++S + ++ E S ++ ++ +D C K S K DI ++D S + ++ F +R + Q +G ++ + L+ F++ +EEF + Y D+ + + S++ + T KALN + F+ S+ + + ++++T+G+S + V A L++ + I IG+G+ + L+I + F ++F+ +SL ++ + C + Q C N D+ VD ST + + +R L+ +Q L
Sbjct: 830 IPESTRQST----------QEGCESVEKADIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTTGKALSKMVPVFQNTARIDVARYLIVITDGQSTDPVAE---AAQGLRDIGVNIYAIG-----VRDANTTELEEIASKKMFFIYEFDSLKSIHQ--EVIRDICSSENCK---SQKADIIFLIDGSESIAPKDFEKMKDFMERMVNQSNIGADEIQIGLLQFSSNP--QEEFRLNRYSSKVDMCRAILSVQQMSDGTHTGKALNF-TLPFFDSSRGGRPRVHQYLIVITDGVSQDNVAP--PAKALRDRNIIIFAIGVGNVQRAQLLEITNDQDKVFQEENFESLQSLEKEILSEVCSS------QGC--NIDLSVGVDTSTSSERAQQE-LRRLLPELMQQL 1241 HSP 6 Score: 63.929 bits (154), Expect = 1.297e-8 Identity = 50/180 (27.78%), Postives = 91/180 (50.56%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEID--RILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
DI ++D S SI +F + + + F I P+ R + Y D + ++F T++ S L+ A+ ++VGG ++ G+AL+ +VP + T+ ID R L++++DG +TD V + A +++ GV IY AIG L + K + EF +++ + ++ IC
Sbjct: 849 DIYFLIDGSGSIKPNDFIEMKDFMKEVIKMFHIGPDRVRFGVVQYSDKIISQFFLTQYASMAGLSAAIDNIQQVGGGTTT-GKALSK----MVPVFQNTARIDVARYLIVITDGQSTD--PVAEAAQGLRDIGVNIY--AIGVRDANTTELEEIASKKMFFIYEFDSLKSIHQEVIRDIC 1019
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|A6NMZ7|CO6A6_HUMAN (Collagen alpha-6(VI) chain OS=Homo sapiens OX=9606 GN=COL6A6 PE=1 SV=2) HSP 1 Score: 166.392 bits (420), Expect = 8.500e-40 Identity = 225/931 (24.17%), Postives = 415/931 (44.58%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKH-------VGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPF-LR--QDFXXXXXXXXXXXXXXCKTTPA----IKPQTCS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRAR-QVFKIDYRA----DVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMI----------CDAFKQR----QDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--------NFESSIEDMTTSTNGVFIGKALTSARVLFKS--DTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQ-LDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL-------RECKNELLNSTRKEVPKVIVLVTDGVS 4163
D+ +VD S ++ + +F + I + +K R +L ++++ + EF H+ + L H +GG +I KAL A R+++ N + ++V+L + S + V+ +A+ L++ GV I ++G+ KA E LK ++T F LR +D + +K K I + C S D++F++D S + + E +D ++ + ++ +L+I R+ ++ YS+ET L K ++ I+ L A+ + R +VF + V +I VL T + + +D++++A + L+ +GV I +G+ S E +AS + + + + + F +R + C ++ D+ + ID S S +F F++ +V ++ + R+G + L I+ D+ K I I + GNT G A+ N T L K ++ VP LVVLT+G + +S A + + I++ ++G+ ++ ++ +L +IA GE V D + K I N+ Q CK D++F++ SS + E F +M FMK+L + + + ++ +++F+ N KE+++ + ++I+ M G AL+ F R N K +I+I DGE D + E A +L+++G+ ++++G+ G + P+ +V+NF LQR I +L I +EC + LD++F +DSS SI D + DF+ +V+ D+ N + G + + D F LD F K +IS ++ G+T EAL E + LN K VP+V++++TDG S
Sbjct: 27 DVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQYSDK--LHSEF-HL-STFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLASSESEDNVE--EASKALRKDGVKIISVGV-QKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYKEGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHR---DSEDNVTKAAVNLRREGVTIFTLGIEGASDTQL-EKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAAL-NFTLSLLQKAKKQRGNKVPCHLVVLTNGMSK--DSILEPANRLREEHIRVYAIGI-KEANQTQLREIA--GEEKRVYYVHDFDAL--KDIRNQVVQEICTEEACKEMKADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDIN------KEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVK--EPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQR----IEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLN---KGVPQVLIVITDGES 923 HSP 2 Score: 164.081 bits (414), Expect = 5.068e-39 Identity = 230/996 (23.09%), Postives = 439/996 (44.08%), Query Frame = 3
Query: 1002 DLIFVLDAS-SSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFI-----SKGREGAKKTVILVT--DGADNSPTMKPEVEAEKLKKMGIRVIVIGL-GLSVIKDQLNKMSSTETYYPIED-MSKMALF-------------------DTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI--DYRADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNA-----TDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNA---KTISNEA---CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNR--------GNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKN--ATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELC 3785
D++F+LD S + +D F + +L I +VG++ YS+ ++ L NK+E+ + + L G + A+++ +E S+ +G + +LVT D DN A L++ G+ + +G+ G S QL K++S +P E +SK+ F T + + + K + + + DI L++D S Q ++ F + F + +K R V +A+ +E E + D+ K +E+++ +GG T ALN + S K+K + +V+LTNG+S + + ++ A L+E + + IG+ + + +I + DF K + + V+ C T A K DI+F+VD S E + +++ M + I +I ++ +SD EF+L+ ++ DI AI Q+ G +AL+ Q F R ++ K ++L TD +D + E A +L+ +GV I VG+ ++ E + +EN + + + ++ + IC ++EC ++ LDV+ ID S S+ + + I DF+ +V+ D+ K++ R G + + + +D D ++ I ++ + A+G + A +D++FT+ R + VP+VL+V+TDG + + +A + GI +++VG+ + L SS +++ V+TF L+ + ++ N + C+I + DL+F+M S + FK+M +F+ S+ + V R+ +F+ G +I K IE++ IG AL F+ D +R N + ++++ DG+ D E + A L+ GI ++++G+ + ++ + +K L V NF L++ N I +C
Sbjct: 229 DVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVINSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTKA-----AVNLRREGVTIFTLGIEGAS--DTQLEKIAS----HPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNF-TLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSI--LEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEIC-TEEACKEMKA----DIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITD---GEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSP----REECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLD------DFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKVDCEIDKV--------DLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGE------KEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQD--EVAQAAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNIC 1176 HSP 3 Score: 145.976 bits (367), Expect = 1.405e-33 Identity = 207/949 (21.81%), Postives = 425/949 (44.78%), Query Frame = 3
Query: 987 NNQQLDLIFVLDAS-SSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKT---VILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTE--TYYPIEDMSKMALFDTTD-IAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT----HIYDDILKKVESLKHVGGFTRIDKALNLASRSFYL---KSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP-FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDY----LFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGD---LIFVMGSSANKTSERFK-----------QMSDFMKSLTKLTVVDKE---NTRVAILKFNRGNYID---SGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVF-TIGLTGKTSESDL 3695
+ ++ D+ ++D S S+ A + + F + V+ I +VG + Y+D ++ + NK ++ K + + + G T AL + ++ K ++++T+G ++P A +L++ IRV IG+ + QL +++ E YY + D AL D + + Q+ C K + K DI +VD S + + F K + + ++G ++ + +V F++ + +EEF DI ++ + H+G T AL+ S+ F N ++ ++L+T+G + + V+ + A L++ GV I ++G+ ++ +L+ +S P F ++F + + + V C K D++FV+D S + ++ ++ M+ ++ ++ + R + Y+D+ F LD+ TK ++ ++ ++ G AL + +F + R V +++++ TD + + A+ L+ G+ ++ VG+ +P S +E + + +C++ K +C + K+D++ +D STS+ P +F+ +F+ +VQ+ D+ +R RIG F+ + P+ + ++ +I I I GNT IG A+ Y + ++ P+VL+VLTDG + + A++ + GI I SVG+G + +++L I + E V F +L+K N + + N IC AG+ + V+ T E+ + + D +++++ L V E T+V++ F N ++ F+ +N +S++D+T + L S F++ + K V++ DG D + ++++ L+K+G+ T+ L G SDL
Sbjct: 431 DTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEP---ANRLREEHIRVYAIGI-KEANQTQLREIAGEEKRVYY-VHDFD--ALKDIRNQVVQEICTEEACKEM---KADIMFLVDSSGSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSD--INKEEFQLNRFMSQSDISNAIDQMAHIGQTTLTGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVK--EPAVVLRQEGVIIYSVGVFG-SNVTQLEEISGRPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVL---DVVFVIDSSGSIDYDEYNIMKDFMIGLVKKADVGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMF-TEARGSRLNKGVPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVTASVCNSSKV---DCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHPEFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQAAEALRHR--GIDIYSVGIG-DVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRN----------ICTTAGESNCFVDVVVGFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSV-AFQVTNAMEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARG-KVVLLFSDGLDDDVEKLEQKSDELRKEGLNALITVALDGPADSSDL 1342 HSP 4 Score: 58.151 bits (139), Expect = 6.944e-7 Identity = 44/185 (23.78%), Postives = 90/185 (48.65%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIV-PEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVKYTK 701
DI +++D S S +F +++ + F I P+ RV + Y D+ EF K+++ L A+ R++GG+++ G ALN TL + + + +++ L++L++G + D S+ + A +++E+ + +Y + I L +K V +F ++ +V IC + K
Sbjct: 436 DIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEINKYSNKQDLGKAIENIRQMGGNTNT-GAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKD--SILEPANRLREEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACK 617
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|P12111|CO6A3_HUMAN (Collagen alpha-3(VI) chain OS=Homo sapiens OX=9606 GN=COL6A3 PE=1 SV=5) HSP 1 Score: 161.384 bits (407), Expect = 3.608e-38 Identity = 266/1144 (23.25%), Postives = 481/1144 (42.05%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIG-LGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT--HIYDDILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXX-------XXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLR-SNGNGGDLNTALNRARQVFKIDYRAD-----VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---------TKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQ----------KPTICKAAG---DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN--------GVFIGKALTS-ARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYI--------SSELCDVIKGTN---KECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELL---NSTRKE--VPKVIVLVTDGVS 4163
D++F++D SS+L L ++ F VI+ L+I QV V Y+D + + K E+ V K++ L+G+ F+ N L S + + EG K ++L+T G +P A++LK+ I IG G + + S+ + P E + + + + S K DI ++D S+ V + F + ++G + R LV F++ V E DIL + L+ GG AL+ + + ++ S+ +L++LLT G S + +QAA L +G+ +G +A++ EL+ ++ P+ +L DF +L V + P +P+ S DI+F+ D S + + + +R + + LN+ +RI++ YSD+ E R DE +K +I +K+++ G +L AL+ A++ + V + +VL +++ D A LK GV + F+ +KN+ E + + I + + + + DV+ +D S + F + +FV R+V++LD+ +DR R+ + ++ E YLN S D+ + ++ + G T G A +++ ++ E VP++L+VLT A+ G+ + +V K GG I +G+G E+ I+ +F + TF L + IS Q+ + +P G D++F++ S + E F+ + ++ L V + TRVA+++F+ D E N SS +++ + + +G AL +R +FK S + + +++I G+ D E + A LK+ G+ FTI E +K SP+ V F L + S ++ ++ T + D++F +DSS + PD + DFV RIVR ++I + +VGVV F+ D EF L + + ++ A++R+ G+ + G+AL L +R E VP+ +VLV G S
Sbjct: 445 DIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGY--RAAEGIPKLLVLITGGKSLDEISQP---AQELKRSSIMAFAIGNKGADQAELEEIAFDSSLVFIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAGQSED--SYLQAANALTRAGILTFCVG-ASQANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPE---SKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGSEGVR-SGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLN----SYMNKQDVVNAVRQLTLLGGPTP---NTGAALEFVLRNILVSSAGSRITEGVPQLLIVLT--ADRSGDDVRNPSVVVKRGGAVPIGIGIGNA-DITEMQTISFIPDFAVAIPTFRQLGTVQ-QVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPE-FQYVRTLIERLVDYLDVGFDTTRVAVIQFS-----DDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDD--EVDDPAVELKQFGVAPFTIARNADQEEL-VKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPAVESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSNDVFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKS 1552 HSP 2 Score: 129.413 bits (324), Expect = 1.694e-28 Identity = 251/1144 (21.94%), Postives = 480/1144 (41.96%), Query Frame = 3
Query: 1002 DLIFVLDASSSL-ALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT---------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG--------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSA----KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---TH-IYDDILKKVESLKHVGG-----FTRIDKALN--LASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIG--LGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTA-------LNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXX-----XXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY----NVKTFTDLEK--FN-AKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFI--------GKALTSA-RVLFKSDTRKNATKTVIIIIDGEITDR--NETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYIS-----------SELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALREC-----KNELLNSTRKEVPKVIVLVTDGVS 4163
D+IF++D S++ ++ + L F N+++ L I + +VGV+ +SD L + K ++ V L G + N R+G S+ EG + ++L++ G + E+ + V GLG L I N + + + D+ + L +AQ + P + K DI +VD SS + L + F + I++ E+G + + ++ +A+ V EF TH +++ V +K + G + +D N S + Y ++ +L+VL+T G S++ + Q A +L+ S + IG D+A+ EE+ S+ F+ +F+ ++ T + P+ S+ DIIF++D S + + +R ++ + SL+I R+ ++ +SD TEF L+ TK DI ++QL+ G G LNT N + R V ++++L T ++ L A L G+ VG S+ + E +A S + + + + + ++ K D++ D S +L + F + DF+ +I+ L+++ + TRI + ++ +V + D + ++ + ++ G +G+A+ A Y+F K + V + LV+L G ++D + P A K G+ + + + EL I S F ++ DL N K++ N A P D++F++ S S F + +F++ + + V ++ RVA+++ Y D E Y N + +D+ + + + G AL R + S T+ V ++ DR ++ + ++K+ G IG+ G +++++++ PD A+ + F L IS S L V++ + D++F +D S S GP++ Q +ER+V +D+ + T+V V+ F+ D + EF L+ + K + +AV+R+ G ++G AL K L + + VP+ +VL++ G S
Sbjct: 242 DIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGG---SRVEEGVPQVLVLISAGPSSD-----EIRYGVVALKQASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQYAD--TVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKSLDEIS--QPAQELKRSSIMAFAIGNKGADQAELEEIAFDSSLV--FIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKRDIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSG-LNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAG--QSEDSYLQAANALTRAGILTFCVGASQANKAEL-EQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQPESKRDILFLFDGSANLVGQ-FPVVRDFLYKIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDGP---ASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGA------PAPVSGEKDVVFLLDGSEGVRSG-FPLLKEFVQRVVESLDVGQDRVRVAVVQ-----YSDRTRPEFYLNSYMNKQDVVNAVRQLTLLGGPTPNTGAALEFVLRNILVSSAGSRITEGVPQLLIVLTADRSGDDVRNPSVVVKRGGAVPIGIGI-GNADITEMQTISFIPDFAVAIPTFRQLGTVQQVISERVTQLTREELSRLQPVLQPLPSPGVGGKRDVVFLIDGSQSAGPEF-QYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIFKRPLGSRIEEGVPQFLVLISSGKS 1348 HSP 3 Score: 115.546 bits (288), Expect = 2.963e-24 Identity = 175/752 (23.27%), Postives = 316/752 (42.02%), Query Frame = 3
Query: 2088 TTPAIKPQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF---KIDYRAD--VAKIVVLFTDKLPTNQKDSLS--EAEILKGDGVKIIFVGLS----------KNSPYNFYE-NLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEK-ELNDIASSGEFY----NVKTFTDLEKFNAKTISNEACQ--IPQKPTICKAA-----GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTST--------NGVFIGKALTSARV-LFKSDTRKNAT----KTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDV--IKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGAT-DIGEALRECKNELLNST-----RKEVPKVIVLVTDGVS 4163
TT A + Q NG DIIF+VD S E + +R + ++SL + +++ ++ TEF L+ TK+++ I + G L Q RA V +++V+ TD + KD L+ AE+ D V + +G+ + P N + NL + S I + D + +D D+I ID S + G NF + LDF+ +++ L + + R+G+ F+ E +D+ A + + + F G A IG A+ + FT+ E VP+VLV+++ G + S +I L + S G+G Q + + EL IA+ ++F DL++ I A + + + PTI D++F++ S+ F + DF+ + + + ++ +VA+ + Y D+ E Y N + ++ T+ + ++ G AL R LF S A K +++I G+ D E + A LK+ I F IG G +++L+ +A +F+ + L + + GT + S K+ D++F LD S+++G + DFV +V +DI + +VG+V F+ EF L+ + K ++ ++++ G+ + G AL R+ VP++++L+T G S
Sbjct: 22 TTHAQQQQADVKNGAAADIIFLVDSSWTIGEEHFQLVREFLYDVVKSLAVGENDFHFALVQFNGNPHTEFLLNTYRTKQEVLSHISNMSYIGGTNQTGKGLEYIMQSHLTKAAGSRAGDGVPQVIVVLTDG---HSKDGLALPSAELKSAD-VNVFAIGVEDADEGALKEIASEPLNMHMFNLENFTSLHDIVGNLVSCVHSSVSPERAGDT-ETLKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPS----SDEIRYGVVALKQASVFSFGLGAQAASRAELQHIATDDNLVFTVPEFRSFGDLQEKLLPYIVGVAQRHIVLKPPTIVTQVIEVNKRDIVFLVDGSSALGLANFNAIRDFIAKVIQRLEIGQDLIQVAVAQ-----YADTVRPEFYFNTHPTKREVITAVRKMKPLDGSALYTGSALDFVRNNLFTSSAGYRAAEGIPKLLVLITGGKSLD--EISQPAQELKRSSIMAFAIGNKG-ADQAELEEIAFDSSLVFIPAEFRAAPLQGMLPGLLAPLRTLSGTPEVHSNKR-DIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGGSRIREHVPQLLLLLTAGQS 755 HSP 4 Score: 82.8037 bits (203), Expect = 2.458e-14 Identity = 114/487 (23.41%), Postives = 218/487 (44.76%), Query Frame = 3
Query: 966 STPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF---------ISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-STETYYPIEDMSKMALFD-------TTDIAQDACQIPKPKRICSAKLDIALIVDCSSQ-VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--THIYDDILKKVESLK-HVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL 2342
TP+ + N + D+IF+LD S+++ + F N++ SL I +VG++ +SD L + Q K++I + +L+L G+ +N +G S+ RE + ++L+T G ++ A L + GI +G K +L +++ + Y ++D S + TT ++ ++P + +K DI + D S+ V V L+ + I++ + TR ++ ++++ VE F +IL V+ +K G + AL+ A R ++KS S+ + +VLL G S +RV A +L++SGV + I AD EL+ + P L + + L + K+ P S D++F++D S + + ++ + ++SL++ + R++++ YSD T EF L+ K+D+ A++QL
Sbjct: 629 GTPEVHSNKR--DIIFLLDGSANVGKTNFPYVRDFVMNLVNSLDIGNDNIRVGLVQFSDTPVTEFSLNTYQTKSDILGHLRQLQLQGGSGLNTGSALSYVYANHFTEAGG---SRIREHVPQLLLLLTAGQSEDSYLQ---AANALTRAGILTFCVGAS-QANKAELEQIAFNPSLVYLMDDFSSLPALPQQLIQPLTTYVSGGVEEVPLAQ--PESKRDILFLFDGSANLVGQFPVVRDFLY--KIIDELNVKPEGTRIAVAQYSDDVKVESRFDEHQSKPEILNLVKRMKIKTGKALNLGYALDYAQRYIFVKSAGSRIEDGVLQFLVLLVAGRSSDRVDG--PASNLKQSGV-VPFIFQAKNADPAELEQIVLSPAFILAAESLPKIGDLHPQIVNLLKSVHNGAPAPVSGEKDVVFLLDGS-EGVRSGFPLLKEFVQRVVESLDVGQDRVRVAVVQYSDRTRPEFYLNSYMNKQDVVNAVRQL 1098 HSP 5 Score: 81.2629 bits (199), Expect = 7.852e-14 Identity = 111/500 (22.20%), Postives = 213/500 (42.60%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFISKG----------REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMS-------STETYYPIEDMSKMALFDTTDIAQDACQ-------IPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD---ILKKVESLKHVGGFT-RIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDK-ADREELKILSTFPN-PFLRQDFXXXX-----------XXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGN 2357
D++F++D S S + ++ L + ++V V+ +SD ++ L + +K E+Q V +L G + NAL E++S+ EG + ++L++ G + P VE LK+ G+ I + +++L K+S S T+ + + + L T + + Q P P + S DI ++D S V+ + + F R + + +G +K R +V F+N+ V E + Y +L + L+ GG KAL +R+ ++KS S+ + +VL+ G S + V A ++ SG I ++G+GD+ DR EL+ ++ P F ++F+E T P +P+ DI+F++D S + + + + + + ++ + ++ ++ Y+ + T EF L + TKR I AI ++ G
Sbjct: 1233 DVVFLIDGSQSAGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNAL-----EYVSRNIFKRPLGSRIEEGVPQFLVLISSGKSDDEVDDPAVE---LKQFGVAPFTI--ARNADQEELVKISLSPEYVFSVSTFRELPSLEQKLLTPITTLTSEQIQKLLASTRYPPPA-VESDAADIVFLIDSSEGVRPDGFAHIRDFVSRIVRRLNIGPSKVRVGVVQFSND-VFPEFYLKTYRSQAPVLDAIRRLRLRGGSPLNTGKALEFVARNLFVKSAGSRIEDGVPQHLVLVLGGKSQDDVSRF--AQVIRSSG--IVSLGVGDRNIDRTELQTITNDPRLVFTVREFRELPNIEERIMNSFGPSAATPAPPGVDTPPPSRPE--KKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEDGDSIQVGLVQYNSDPTDEFFLKDFSTKRQIIDAINKVVYKGG 1714 HSP 6 Score: 76.2554 bits (186), Expect = 2.855e-12 Identity = 84/331 (25.38%), Postives = 144/331 (43.50%), Query Frame = 3
Query: 3258 AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE---SSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNA--------TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKE-----------CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALR---ECKNELLNSTRKE--VPKVIVLVTDGVST 4166
AA D+IF++ SS E F+ + +F+ + K V + + A+++FN GN Y+ + S I +M+ GK L + +S K A + ++++ DG D A L D + VF IG+ E LK +AS + + N N + + + + V + E + D++F +D S++ G + LDF+ ++ + I +VGVV F+ + R F LD ++ K ++ AVK + F G +IG AL E +R E VP+V+VL++ G S+
Sbjct: 36 AAADIIFLVDSSWTIGEEHFQLVREFLYDVVKSLAVGENDFHFALVQFN-GNPHTEFLLNTYRTKQEVLSHISNMSYIGGTNQTGKGL---EYIMQSHLTKAAGSRAGDGVPQVIVVLTDGHSKD-GLALPSAELKSAD-VNVFAIGVE-DADEGALKEIASEPLNMHMFNLENFTSLHDIVGNLVSCVHSSVSPERAGDTETLKDITAQDSADIIFLIDGSNNTGSVNFAVILDFLVNLLEKLPIGTQQIRVGVVQFSDEPRTMFSLDTYSTKAQVLGAVKALGFAGGELANIGLALDFVVENHFTRAGGSRVEEGVPQVLVLISAGPSS 359 HSP 7 Score: 58.5362 bits (140), Expect = 6.432e-7 Identity = 45/157 (28.66%), Postives = 75/157 (47.77%), Query Frame = 3
Query: 108 VILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSE-----IDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
V+ L +G RD+ ++D S S EFQ I + + + + + TRV+ + + D K EFL H+S + AV+ R GG +G AL +I RP + + L+++S G + DE V D A ++++ GV +T+A
Sbjct: 1219 VLQPLPSPGVGGKRDVVFLIDGSQS-AGPEFQYVRTLIERLVDYLDVGFDTTRVAVIQFSDDPKVEFLLNAHSSKDEVQNAVQRLRPKGGRQINVGNALEYVSRNIF--KRPLGSRIEEGVPQFLVLISSGKSDDE--VDDPAVELKQFGVAPFTIA 1370 HSP 8 Score: 57.3806 bits (137), Expect = 1.517e-6 Identity = 38/130 (29.23%), Postives = 65/130 (50.00%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVSTGGL---SPELEST 4196
D++F +DSS +IG +++Q +F+ +V+ + + N +V FN + EF L+ + K ++S + + + G G+ L L VP+VIV++TDG S GL S EL+S
Sbjct: 39 DIIFLVDSSWTIGEEHFQLVREFLYDVVKSLAVGENDFHFALVQFNGNPHTEFLLNTYRTKQEVLSHISNMSYIGGTNQTGKGLEYIMQSHLTKAAGSRAGDGVPQVIVVLTDGHSKDGLALPSAELKSA 168
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Match: sp|A8TX70|CO6A5_HUMAN (Collagen alpha-5(VI) chain OS=Homo sapiens OX=9606 GN=COL6A5 PE=1 SV=1) HSP 1 Score: 148.673 bits (374), Expect = 2.311e-34 Identity = 248/1130 (21.95%), Postives = 491/1130 (43.45%), Query Frame = 3
Query: 1002 DLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSK-LELLEGATFISNALRRSGEEFIS---KGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALF--DTTDIAQDACQIPKPKRICSAKLDIALIVDCS--SQVQLHEWVNQLL-----------FAKRFIEQFELGINKTRFSLVSFANEA---------VVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQ-------------TC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKI---VVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL-FTKG-REDVPKVLVVLTDGA--NDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS-SGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG--DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSG----FKEDYKNFESSIEDMTT--STNG-VFIGKALTSARVLF----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKE--VPKVIVLVTDG 4157
D++F++D+S L + F N +I SL I+ +V + YSD L + + ++ + + K + + G+ I AL+ + + S GR+ + ILV + S + EVE ++ L+K G+++I +G+ ++ L M+++ ++ + + ++ F + T I +D + + A + + + C S L V++ L F + ++ N R L+S++N A + EF +++++L G + A++ R + +S S+R + VL+T+ S + V AA +L+ V++ + + A+ +L+ + ++P K L S K K I Q C + DI F++D S+ + ++ I+ ML + +I R+ ++ YSD+T EF + + D+ AI ++ G AL+ Q+ K + ++K+ +++ TD + T++ + A+ L+ + + + VG+ + E ++ +N + + K ++ IC + C K D++ +D S S+G ENF+ F+ ++ + + D+T+IG+ F+ + ++ F ++ I+++ I T G A+ Y +KG R K L+++TDG +D+ + +I K + I SVGV + +L +I+ S ++V+ F L+ K I C + CK D++FV+ S + KQ D M +LT + +V K + ++F Y D + Y N + IE++ T G + KAL A LF S ++N + +I+I DGE D ++ + A L+ GI +F +G+ GK ++ +L+ +A + + ++VDNF L+ + +C + C ++ D++F D S + + T F+ ++ + DI + K+G+ F ++ + L K + ++ + G I AL++ N L R+ VP+ +V++T G
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAES---EDEVEEASKALQKDGVKIISVGV-QKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKE--GAVDADMQVHFPISCQKDSLADLVFLVDESLGTGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQ-------QQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSDDEVH--DAALNLRLEDVNVFALSI-QGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDR--VVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC-----AEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVAQDDVRDPARILRGKD----VTIFSVGV-YNANRSQLEEISGDSSLVFHVENFDHLKALERKLIF-RVCALHD----CKRITLLDVVFVLDHSGSIK----KQYQDHMINLT-IHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDGESHDHDQLNDTALELRNKGITIFAVGV-GKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMCTE---APEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSG 1117 HSP 2 Score: 137.887 bits (346), Expect = 4.614e-31 Identity = 167/729 (22.91%), Postives = 336/729 (46.09%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSN----GNGGDLNTALNRARQVF---KIDYRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQD------------ECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNA-----TDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS---SGEFYNVKTFTDLEKFNAK-----------TISNEACQIPQKPTIC--KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKT---VIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKAL-FVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVS 4163
D++F+VD S + + +++ + + SL I R+++ YSDE +EF L F R + L+ N G + AL A + + I+ R D + + +D + EA + L+ DGVKII VG+ K S N + +A+ + I + + + +I D K ++ C L D++ +D+S G N + F+ I ++D++++ R+G+ ++++ + S+ ++ Q++I + G + G AI ++ ++ + VP++ V++T +D + +A+ +L + + ++ + Q + +L +I S +K++ DLE ++ K IS A Q T C D+ F++ S++ ++F+Q+ FM +T++ + + RV +++++ ++ + DY N +I ++ T G + GKAL + K+ + +K +I++ DG TDR E A L+ + I V +G+ G ++ +L+ +A ++ + F NF L+ + E+C K C + D+MF +DSS SIG + ++ F++ ++ + I A+ T++GVV F+ ++EF L+++ + + A+ R+ + T G+AL +S R K ++L+TDGV+
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHL-STFKGRS--PMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGR-DRKQFPPILVVLASAESEDEVEEASKALQKDGVKIISVGVQKASEENL-KAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLGTG-GNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSD--DEVHDAALNLRLEDVNVFALSI-QGANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEF-YITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDR--VVEPAKRLRAEQITVHAVGI-GAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC-----AEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQYFTHSKGARLGAKKFLILITDGVA 740 HSP 3 Score: 107.457 bits (267), Expect = 7.604e-22 Identity = 176/794 (22.17%), Postives = 354/794 (44.58%), Query Frame = 3
Query: 948 SNYIEISTPDQ-NC-NNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTV----ILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT-----TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY--DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK----NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-PFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY----RADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDY--LFTKGRED--VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFN----AKTISNEACQ 3230
S Y E D+ C + ++ D+ F++D SSS+ ++Q F V + I +VGV+ YSD E+ + N +++K + ++ L G T+ AL + I G + V I++TDG ++P A++L+ I V +G+G + NK+ E E +S FD ++ ++ C ++ C K DI +VD S + + +F K + + ++G +KT+ +V F+++ E + + +I ++ + + T KALN + ++ SK +K+ ++L+T+G++ + V+ A L+ V I ++G+ + A+R +L+ +S + F ++F K+L K + C + D++FV+D S + D + +L + ++ ++ R + + YSD+ F L+ + I +++ R G AL A +F ++ + +V +++++ TD ++ D L++ + L+ G+ I VG+ K + +K ++I ++N +K+ ++ +C + + C +++ DVI D S + +F F++ ++ N D++ R +IG+ F S + + ++ + +I + G I +A+ ++ L GR + VP+ LVV+T G + + +K GI ++ +G+G E L +G + TF D +K K I E CQ
Sbjct: 422 STYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYI-LQIIKNGMKDRMSKVPCYLIVLTDGMSTDRVVEP---AKRLRAEQITVHAVGIGAA------NKIELQEIAGKEERVSFGQNFDALKSIKNEVVREIC----AEKGCEDMKADIMFLVDSSWSIGNENFRKMKIFMKNLLTKIQIGADKTQIGVVQFSDKTKEEFQLNRYFTQQEISDAIDRMSLINEGTLTGKALNFVGQ-YFTHSKGARLGAKKFLILITDGVAQDDVR--DPARILRGKDVTIFSVGVYN-ANRSQLEEISGDSSLVFHVENFDHLKALERKLIFRVCALHDCKRITLL----DVVFVLDHSGSIKKQYQDHMINLTIHLVKKADVGRDRVQFGALKYSDQPNILFYLNTYSNRSAIIENLRKRRDTGGNTYTAKALKHANALFTEEHGSRIKQNVKQMLIVITDG-ESHDHDQLNDTALELRNKGITIFAVGVGKANQKELEGMAGNKNNTIYVDNFDKLKDVFTLVQERMC---TEAPEVCHLQEADVIFLCDGSDRVSNSDFVTMTTFLSDLIDNFDIQSQRMKIGMAQFGSNYQSIIELKNSLTKTQWKTQIQNVSKSGGFPRIDFALKKVSNMFNLHAGGRRNAGVPQTLVVITSGDPRYDVADAVKTLKDL--GICVLVLGIGDVYKEHLL---PITGNSEKIITFQDFDKLKNVDVKKRIIREICQ 1184 HSP 4 Score: 105.916 bits (263), Expect = 2.163e-21 Identity = 124/534 (23.22%), Postives = 245/534 (45.88%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINK-IPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF--NAKTISNEACQIPQK----------PTICK--AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED--YKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT----RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS--PDKAL-FVDNFSNLQRYNAY--------ISSELCDVIKGTNKE---C-STKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEA----LRECKNELLNSTRKEVPKVIVLVTDGVST 4166
DV+ +D S LGP++F F+N+++ +L + ++ R+ + ++ E + + + + M + K FI G+ IG A+ A F+ + R+ P +LVVL ++ E + S K G+KIISVGV Q+ SE+ L +A+S +N++T DL F N I + + + P C+ + DL+F++ S T + + F++++T V + R+ ++ ++ S K F+ I++++ G A+ R S++ R + +++ +D +E + A L+ + + VF + + G + + L+ + S P++ + + ++++L+ Y+ I S++ + N + C TK+ D+ F +D SSSI ++ F+ + I + +VGVV ++ D EF + ++ + A+ I G T G+A L+ KN + + K VP ++++TDG+ST
Sbjct: 30 DVVFLVDSSDHLGPKSFPFVKTFINKMINSLPIEANKYRVALAQYSDEFHSEFHLSTFKGRSPMLNHLKKNFQFIGGSLQIGKALQEAHRTYFSAPINGRDRKQFPPILVVLASAESE-DEVEEASKALQK-DGVKIISVGV-QKASEENLKAMATSHFHFNLRTIRDLSTFSQNMTQIIKDVTKYKEGAVDADMQVHFPISCQKDSLADLVFLVDESLG-TGGNLRHLQTFLENITSSMDVKENCMRLGLMSYSNSAKTISFLKSSTTQSEFQQQIKNLSIQVGKSNTGAAIDQMRRDGFSESYGSRRAQGVPQIAVLVTHRPSD-DEVHDAALNLRLEDVNVFALSIQG-ANNTQLEEIVSYPPEQTISTLKSYADLETYSTKFLKKLQNEIWSQISTYAEQRNLDKTGCVDTKEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTGGTYTGKALDYILQIIKNGMKDRMSK-VPCYLIVLTDGMST 556 HSP 5 Score: 56.225 bits (134), Expect = 3.345e-6 Identity = 49/185 (26.49%), Postives = 89/185 (48.11%), Query Frame = 3
Query: 141 KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRP-TSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVK 692
K DI ++D SSSI +F+ + + F I P+ RV + Y D + EF T +++ L A+ +++ G + G+AL+ L I M+ S++ L++L+DG +TD V + A +++ + +T++ V IG+ I L ++ V F ++ +V IC K
Sbjct: 439 KEADIHFLIDGSSSIQEKQFEQIKRFMLEVTEMFSIGPDKVRVGVVQYSDDTEVEFYITDYSNDIDLRKAIFNIKQLTG-GTYTGKALDYILQIIKNGMKDRMSKVPCYLIVLTDGMSTDR--VVEPAKRLRAEQITVHAVGIGAANKIELQEIAGKEERVSFGQNFDALKSIKNEVVREICAEK 620
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A1S6KME9 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 2703.7 bits (7007), Expect = 0.000e+0 Identity = 1436/1440 (99.72%), Postives = 1438/1440 (99.86%), Query Frame = 3
Query: 57 MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVRNXXXXXXXXXXXXIIRTTIPDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIPFETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKKA 4376
MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGSNLNIPLLLSISSKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVRNTTTATSKQTETTIIRTTIPDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIP ETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD+AQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALN+ASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDI TIGLGDKADREELKILSTFPNPFLRQDFKEEKSLLEKSVKVSCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNISRTTSRISIIDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLASKKSSIKIENINKIDKNKEQIKNMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTLIKKLGIKIIVIGIGDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKKA
Sbjct: 1 MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGSNLNIPLLLSISSKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVRNTTTATSKQTETTIIRTTIPDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIPVETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDLAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNIASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIVTIGLGDKADREELKILSTFPNPFLRQDFKEEKSLLEKSVKVSCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNISRTTSRISIIDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLASKKSSIKIENINKIDKNKEQIKNMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTLIKKLGIKIIVIGIGDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKKA 1440
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A210PQ64 (Collagen alpha-4(VI) chain OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09962 PE=4 SV=1) HSP 1 Score: 306.22 bits (783), Expect = 1.625e-81 Identity = 273/1112 (24.55%), Postives = 517/1112 (46.49%), Query Frame = 3
Query: 972 PDQNCNNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEE-FISK--GREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT-----TDIAQDACQIPKPKRICSAK-LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY-----DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP-NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQV-----FKIDY--RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIP-QKPTICKAAG----DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRG--NYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSAR---VLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKK-DGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS---TRKEVPKVIVLVTDGVST 4166
P + CN ++ D++F+LD+SSS + ++L F N ++ + +Q + +S ++ + L Q ++ + ++ + G T AL + + F++ GR A+K VI++TDG SP+ K + L G+ VI +G+G + + +LN ++S + + +++ FD T++ ACQ P CS K DI ++D SS + ++ QL F K + QF++G + T+ S+VSF++ V +EF H+ +L + ++K+ G T + AL ++ + + ++V++LT+G S TI A L +G ++ IG+G D+ EL ++T P + F Q++ S+ +CKT + DI+F++D S +E ++ M I + ++ + +S T EF L+ + AI ++ NT + A Q F ++ R + I+V+ TD + + S A+++ G+K + +G+ + N + +A+ + + + + ++ EC + D++ +D S+S G NF +L F+ V+ + ++G+ TF++ + ++ + A + ++ I + G T + + + F R + K+ VV+TDG + S A K G+K+IS+G+G I +EL IA+ + ++V +F L+ + + N AC +P Q P + + G D+IF++ SS ++T F + DF+K+ + N +V + F+ I D + + I ++ + AL AR L R NAT V+++ DG+ T +A LLK G+KV +IG+ + +L ++AS +F V NF++L + + I+ C+ ++ D++FA+D+S S G +Q LDF+ + V + + G+VTF T A EF L+Q+++K +++SA+++ ++ G T GEAL+ + L + R ++++TDG ST
Sbjct: 24 PPKQCN-EKADIVFILDSSSSEGTTNFHKQLDFVKNFVQQFDVGPDKTQFSAITFSTHVKNNFWLNEHQTQSSVLSALNNVNYQSGITNTDQALNFARQNAFLANNGGRPDAEKIVIVLTDGQSTSPS-KTSAASNTLHHSGVEVISVGIGNGITQSELNVIASDPQH-----VFQVSSFDALQSIQTELTNAACQAPTAA--CSKKKADIVFVLDSSSSIGSTDFTKQLDFVKNNVRQFDIGPDDTQISVVSFSDS--VFDEF-HLNRFTAKTQVLGAISAIKYHTGTTYTNLALAHVRNETFIAANGGRPDAAKVVIVLTDGQSTSGQATIAEANKLHMTGAEVVAIGIGSGVDKGELNAIATDPAHVFEVQNYDVLSSIQHSLELATCKTN-----ECRGQKADIVFLLDSSASEGSENFNKQLDFMKNFTMQFQIGKDNVQMGLAVFSTLTNNEFWLNTYNDSGSLENAIDNVQYLPG----NTYTDEALQFIVNNSFTAEHGMRDGIPHILVVMTDGQSHDPAATASIAKLVHNAGIKTVAIGIGTDVDKNELQAIATDANHMILVPDFNALATIQSEVEASACKSERPDQECGTQPADIVFILDASSSEGHANFNKQLQFMGDFVKQFQIGPKNVQVGLVTFSTSAHNEFYFNTYHDQASILNKLQNIHYTGGTTHTDKGLQYSRLFHFNSAYHGARRNADKIAVVMTDGQSSSKSSTSRQAQYLKDMGVKVISIGIGSGIDSRELTSIATDTQHAFSVNSFDVLKSIELE-LKNAACGVPTQAPNVDQECGQKLADIIFLLDSSGSETRTNFNKQLDFVKNFASQFQIGPNNVQVGVATFSTHVTEQIKLNAYHDQQALMAGISHVSYDGGLTYTDDALKYARQTGFLPSHGGRSNATHIVVVMTDGQSYSSGLTKTQATLLKNIPGMKVISIGIGSSVNRDELLTIASDSSHMFEVANFNSLSQIGSEITFATCNTCGYDDRA------DMVFAIDASGSEGSTNFQKSLDFMAQFVNSFPVGPGHVQFGLVTFGTHAVNEFDLNQYSDKQSVLSAIQKANYESGTTATGEALKIIREHSLQTPAGARPSSHHFVIVLTDGAST 1107 HSP 2 Score: 188.734 bits (478), Expect = 2.096e-44 Identity = 196/784 (25.00%), Postives = 352/784 (44.90%), Query Frame = 3
Query: 957 IEISTPDQN-CNNQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRR-SGEEFISKG--REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYY-PIEDMSKMALFDTTDIAQDACQIPKPKRICSAK-LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD--ILKKVESLKHVGGFTRIDKALNLASRSFYLKS------KNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILST-FPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKP---QTCSSN-GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQV-FKIDY--RADVAKIVVLFTDKLPTNQKDSLSEAEILKG-DGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMK---------KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---TKGREDVPKVLVVLTDGANDIGESPQISAVKAKL---GGIKIISVGVGQQISEKELNDIAS-SGEFYNVKTFTDL 3188
+E++T N C Q+ D++F+LD+S+S ++++L F N QI Q+G+ ++S + L + + ++ + ++ L G T+ AL+ F ++ R+G ++++TDG + P + A+ + GI+ + IG+G V K++L +++ + + D + +A + ++ AC+ +P + C + DI I+D SS + QL F F++QF++G + LV+F+ A E F +D IL K++++ + GG T DK L SR F+ S +N+ ++ V++T+G S + T + A L++ GV + +IG+G D EL ++T + F F KS+ + +C P P Q C DIIF++D S T ++ + I ++ + +S T + +L+ ++ + I + +G + AL ARQ F + R++ IVV+ TD + + ++A +LK G+K+I +G+ S N E L S + + ++ Q E T + D++ ID S S G NFQ LDF+ + V + + + G+ TF + ++ + + I K + G TA G A+ ++ R ++VLTDGA+ S Q A AKL G +IS+G+G + + ELN IA+ S +N F L
Sbjct: 397 LELATCKTNECRGQKADIVFLLDSSASEGSENFNKQLDFMKNFTMQFQIGKDNVQMGLAVFSTLTNNEFWLNTYNDSGSLENAIDNVQYLPGNTYTDEALQFIVNNSFTAEHGMRDGIPHILVVMTDGQSHDPAATASI-AKLVHNAGIKTVAIGIGTDVDKNELQAIATDANHMILVPDFNALATIQS-EVEASACKSERPDQECGTQPADIVFILDASSSEGHANFNKQLQFMGDFVKQFQIGPKNVQVGLVTFSTSAHNEFYFNTYHDQASILNKLQNIHYTGGTTHTDKGLQY-SRLFHFNSAYHGARRNADKIAVVMTDGQSSSKSSTSRQAQYLKDMGVKVISIGIGSGIDSRELTSIATDTQHAFSVNSFDVLKSIELELKNAACGV-PTQAPNVDQECGQKLADIIFLLDSSGSETRTNFNKQLDFVKNFASQFQIGPNNVQVGVATFSTHVTEQIKLNAYHDQQALMAGISHVSYDGGLTYTDDALKYARQTGFLPSHGGRSNATHIVVVMTDGQSYSSGLTKTQATLLKNIPGMKVISIGIG--SSVNRDELLTIASDSSHMFEVANF------------NSLSQIGSEITFATCNTCGYDDRADMVFAIDASGSEGSTNFQKSLDFMAQFVNSFPVGPGHVQFGLVTFGTHAVNEFDLNQYSDKQSVLSAIQKANYESGTTATGEALKIIREHSLQTPAGARPSSHHFVIVLTDGAST--NSSQTVAEAAKLKQNGNTTVISIGIGSNVDKSELNTIATDSRHVFNAIDFNAL 1160 HSP 3 Score: 142.124 bits (357), Expect = 4.188e-30 Identity = 96/321 (29.91%), Postives = 167/321 (52.02%), Query Frame = 3
Query: 3243 PTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN---GVF-IGKALTSAR---VLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL---NSTRKEVPKVIVLVTDGVSTGG 4172
P C D++F++ SS+++ + F + DF+K+ + V + T+ + + F+ ++ + F + +SS+ + N G+ +AL AR L + R +A K VI++ DG+ T ++T +N L G++V ++G+ ++S+L +AS P V +F LQ +++ C + CS K+ D++F LDSSSSIG + +LDFV+ VR DI + T++ VV+F+ EF L++FT K ++ A+ I + G T AL +NE N R + KV++++TDG ST G
Sbjct: 25 PKQCNEKADIVFILDSSSSEGTTNFHKQLDFVKNFVQQFDVGPDKTQFSAITFST--HVKNNFWLNEHQTQSSVLSALNNVNYQSGITNTDQALNFARQNAFLANNGGRPDAEKIVIVLTDGQSTSPSKTSAASNTLHHSGVEVISVGIGNGITQSELNVIASDPQHVFQVSSFDALQSIQTELTNAAC---QAPTAACSKKKADIVFVLDSSSSIGSTDFTKQLDFVKNNVRQFDIGPDDTQISVVSFSDSVFDEFHLNRFTAKTQVLGAISAIKYHTGTTYTNLALAHVRNETFIAANGGRPDAAKVVIVLTDGQSTSG 340 HSP 4 Score: 115.931 bits (289), Expect = 5.342e-22 Identity = 102/382 (26.70%), Postives = 179/382 (46.86%), Query Frame = 3
Query: 912 KSIPFETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSS-LALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNAL---RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKM-GIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIP-KPKRICSA--KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKS-----KNSKRLVVLLTNGLSMERVQTIQAAYDLQESG-VDIATIGLGDKADREELKILST 2009
KSI E K + DQ C + D+IF+LD+S S ++++L F N QI QVGV +S + + L + ++ + +S + G T+ +AL R++G GR A V+++TDG S + + +A LK + G++VI IG+G SV +D+L ++S ++ M ++A F++ ++Q +I C + D+ +D S + L F +F+ F +G +F LV+F AV E + D +L ++ + G T +AL + R L++ +S V++LT+G S QT+ A L+++G + +IG+G D+ EL ++T
Sbjct: 774 KSIELELKNAACGVPTQAPNVDQECGQKLADIIFLLDSSGSETRTNFNKQLDFVKNFASQFQIGPNNVQVGVATFSTHVTEQIKLNAYHDQQALMAGISHVSYDGGLTYTDDALKYARQTGFLPSHGGRSNATHIVVVMTDGQSYSSGLT-KTQATLLKNIPGMKVISIGIGSSVNRDELLTIASDSSH-----MFEVANFNS--LSQIGSEITFATCNTCGYDDRADMVFAIDASGSEGSTNFQKSLDFMAQFVNSFPVGPGHVQFGLVTFGTHAVNEFDLNQYSDKQSVLSAIQKANYESGTTATGEALKII-REHSLQTPAGARPSSHHFVIVLTDGASTNSSQTVAEAAKLKQNGNTTVISIGIGSNVDKSELNTIAT 1146
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A0L8IBT2 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22021680mg PE=4 SV=1) HSP 1 Score: 276.559 bits (706), Expect = 6.661e-73 Identity = 275/1002 (27.45%), Postives = 493/1002 (49.20%), Query Frame = 3
Query: 1557 SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHI--YDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXC---KTTPAIKP---QTCS-SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL-RSNGNGGDLNTALNRARQV-FKIDY--RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM-ICDA---FKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNA--KTISNEACQ------IP--QKPTIC-KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE-----SSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS---PDKALFVDNFSNLQRYNAYISSELCDVIKGTNK------ECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEA---LRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGT---FYRILDFKTLKDYDTTFITRESCKAGKKA 4376
S+ DI I+D S V ++ + F K ++ F++G +K R ++ FA +V E+F H+ Y D +L + ++K + G T AL A R+ + N R + +++T+G S T+ AA +L+ + + +IG+G + EL+ ++T P + F +F ++ ++ +C K P I P C+ S DI+FV+D S +N + + + + ++ +IS +T R+++ +S T +F LD+ F K + AI Q+ R G+ N AL RAR V F+ + R+ I ++ TD N + A LK G + +G+ N + E++AS KS I K +N ++ + C+A Q C D++ ID S S+G NFQ L FV +V D+ KD RIG+ +F+++ + +++ F + + I+K+P+ +G+T A+G +FT R P + +V+TDG ++ S A + G + S+GVG ++ +ELN++AS + +V T +D F + K ++ +AC+ +P + P +C + D+IF + +SA+ +K M F+ + + + K++ RV ++ F+ + ++ + + E S+++ STN + + + +L ++ R N VI++ DG+ T EA LKK VF+IG+ + +LK +++ D V NF L++ A ++ C+V G NK +C Q D++F LDSS S+G +Q L FVE + + +I + ++GV TF++ +R EF +++ ++ +++ AV +I G T +GEA LRE R V + +++TDG G L+ E+ +K G K+ IGIG S +QL +++S T ++ +F +LK + ++R+SC KA
Sbjct: 37 SSVADILFILDSSGSVSNVDFTKMITFVKEMVKGFDVGKDKIRVAVSLFAGTSV--EKF-HLNKYSDKNALLSAIGAIKRISGNTNTADALTKA-RTVIFTAGNGDRPKVPNIAIVITDGNSNNAQLTVSAANNLKATQAVVFSIGVGTNIRQSELQAIATGPGTSHVFTVTNFAALNTIKKELATKTCEAQKFIPTIMPTDAPDCNVSMADILFVLDSSGSVSNPDFVRMITFVKEMVKGFDISNSTVRVAVSIFSGGTVEKFHLDKYFDKAALLKAIGQISRVTGSTNTAN-ALTRARTVSFQPPHGDRSGAPNIAIVITDGNSDNPIQTTDAALQLKNTGTAVFSIGVGSNVRQSELESIASDKSHIFTVTNFKALENIKKNLAIKTCEAQVIPTQPAITCNQSMADIVFLIDSSGSVGSSNFQKMLQFVKNMVNGFDVGKDTVRIGVASFSNKPVQHFALNTYFNRSQIIDAISKLPYYQGSTNTASALGLLRSSVFTAASGDRPTAPNIAIVITDGQSNDPSSTAAQAKALRQTGTILFSIGVGSGVNARELNNMASDPDKDHVFTVSDFSAFGSIQKELAIKACEAQKYTPLPPTKSPVVCNRTMADIIFALDTSASVDEHSYKLMLTFVHRMVQSFDIGKDDVRVGLVTFSDNSVLEFHLNKYFNASELDKAISNVKYKAGSTNTADALRYIRTISLLPQNGNRPNIPNIVIVLTDGKSNVPASTTAEAMKLKKLA-TVFSIGIGSQAFYRELKDISTDPDADHVFTVSNFKALEKIRATLAVRTCEV--GKNKTEPEIRKCPA-QADIVFILDSSGSVGSANFQKMLRFVETVSKSFNIGPDEVQIGVDTFDSGSRTEFNMNKHKDQQSLLKAVGKIPTRSGMTHMGEALKRLRETSMSKAAGMRSNVSHIAIMITDGRPNGRLNVVQEAKKVKDSGTKLFTIGIGPSVDYNQLNVIASKPTKQFVFKADNFDSLKSI-ASMLSRQSCNGKPKA 1028 HSP 2 Score: 222.631 bits (566), Expect = 1.980e-55 Identity = 232/999 (23.22%), Postives = 454/999 (45.45%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTT--DIAQDACQ--------IPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLA-----SRSFYLKSKNSKR-----LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELK-ILSTFPNPFLRQDFXXXXXXXXXXXXXXCK-----TTPAIKPQTCS-SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRAR-QVFKIDY--RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFK-------QRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEG--NTAIGWAIGNATDYLFTKG-REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS---SGEFYNVKTFTDLEKFNAKTISNEACQIPQ---KPTI--CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGF--KEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFV---DNFSNLQRYNAYISSELCD 3788
CN+ D++F+LD+S S++ + + + + F ++K + +V V +++ L +K + + ++ + G T ++AL ++ + G R I++TDG N+ + A LK V IG+G ++ + +L +++ + ++ A +T ++A C+ +P C+ + DI ++D S V ++V + F K ++ F++ + R ++ F+ V E+F H+ D K LK +G +R+ + N A +R+ + + R + +++T+G S +QT AA L+ +G + +IG+G + EL+ I S + F +FK +++ + +C+ T PAI TC+ S DI+F++D S + + + + + ++ + T RI + +S++ F L+ F + I AI +L + +AL R VF R I ++ TD + + ++A+ L+ G + +G+ N+AS + ++ K + C+A K + C D+I +D S S+ ++++ L FV+R+VQ+ D+ KD R+G+ TF+ L ++ F +++ K I+ + + G NTA L G R ++P +++VLTDG +++ S A+K K + S+G+G Q +EL DI++ + + V F LEK A T++ C++ + +P I C A D++F++ SS + S F++M F+++++K + + ++ + F+ G+ + +D ++ ++ + T + +G+AL R S R N + I+I DG R +EA +K G K+FTIG+ + L +AS FV DNF +L+ + +S + C+
Sbjct: 35 CNSSVADILFILDSSGSVSNVDFTKMITFVKEMVKGFDVGKDKIRVAVSLFAGTSVEKFHLNKYSDKNALLSAIGAIKRISGNTNTADALTKARTVIFTAGNGDRPKVPNIAIVITDGNSNNAQLTVSA-ANNLKATQAVVFSIGVGTNIRQSELQAIATGPGTSHVFTVTNFAALNTIKKELATKTCEAQKFIPTIMPTDAPDCNVSMADILFVLDSSGSVSNPDFVRMITFVKEMVKGFDISNSTVRVAVSIFSGGTV--EKF-HL-DKYFDKAALLKAIGQISRVTGSTNTANALTRARTVSFQPPHGDRSGAPNIAIVITDGNSDNPIQTTDAALQLKNTGTAVFSIGVGSNVRQSELESIASDKSHIFTVTNFKALENIKKNLAIKTCEAQVIPTQPAI---TCNQSMADIVFLIDSSGSVGSSNFQKMLQFVKNMVNGFDVGKDTVRIGVASFSNKPVQHFALNTYFNRSQIIDAISKLPYYQGSTNTASALGLLRSSVFTAASGDRPTAPNIAIVITDGQSNDPSSTAAQAKALRQTGTILFSIGVGSGVNARELNNMASDPDKDHVFTVSDFSAFGSIQKELAIKACEAQKYTPLPPTKSPVVCNRTMADIIFALDTSASVDEHSYKLMLTFVHRMVQSFDIGKDDVRVGLVTFSDNSVLEFHLNKYFNASELDKAISNVKYKAGSTNTADALRYIRTISLLPQNGNRPNIPNIVIVLTDGKSNVPASTTAEAMKLKKLA-TVFSIGIGSQAFYRELKDISTDPDADHVFTVSNFKALEKIRA-TLAVRTCEVGKNKTEPEIRKCPAQADIVFILDSSGSVGSANFQKMLRFVETVSKSFNIGPDEVQIGVDTFDSGSRTEFNMNKHKDQQSLLKAVGKIPTRSGMTHMGEALKRLRETSMSKAAGMRSNVSHIAIMITDGRPNGRLNVVQEAKKVKDSGTKLFTIGIGPSVDYNQLNVIASKPTKQFVFKADNFDSLKSIASMLSRQSCN 1023 HSP 3 Score: 169.088 bits (427), Expect = 1.696e-38 Identity = 143/527 (27.13%), Postives = 246/527 (46.68%), Query Frame = 3
Query: 2682 CTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNS----EVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNA--KTISNEACQIPQK--PTI-------CKAA-GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSAR-VLFKSDT--RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCD---VIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST---RKEVPKVIVLVTDGVS 4163
C D++ +D S S+ +F + FV +V+ D+ KD+ R+ + F + +LN D N + I I I GNT A+ A +FT G R VP + +V+TDG ++ + +A K + S+GVG I + EL IA+ +V T T+ N K ++ + C+ QK PTI C + D++FV+ SS + ++ F +M F+K + K + RVA+ F+ G ++D F D +I ++ T ALT AR V F+ R A I+I DG + +T + A LK G VF+IG+ +S+L+S+AS +F V NF L+ ++ + C+ + C+ D++F +DSS S+G +Q L FV+ +V D+ + ++GV +F+ + F L+ + + +I A+ ++ + G+T+ AL ++ + + R P + +++TDG S
Sbjct: 35 CNSSVADILFILDSSGSVSNVDFTKMITFVKEMVKGFDVGKDKIRVAVSLFAGTSVEKFHLNKYSDKN----ALLSAIGAIKRISGNTNTADALTKARTVIFTAGNGDRPKVPNIAIVITDGNSNNAQLTVSAANNLKATQAVVFSIGVGTNIRQSELQAIATGPGTSHVFTVTNFAALNTIKKELATKTCE-AQKFIPTIMPTDAPDCNVSMADILFVLDSSGSVSNPDFVRMITFVKEMVKGFDISNSTVRVAVSIFSGGTVEKFHLDKYF--DKAALLKAIGQISRVTGSTNTANALTRARTVSFQPPHGDRSGAPNIAIVITDGNSDNPIQTTDAALQLKNTGTAVFSIGVGSNVRQSELESIASDKSHIFTVTNFKALENIKKNLAIKTCEAQVIPTQPAITCNQSMADIVFLIDSSGSVGSSNFQKMLQFVKNMVNGFDVGKDTVRIGVASFSNKPVQHFALNTYFNRSQIIDAISKLPYYQGSTNTASALGLLRSSVFTAASGDRPTAPNIAIVITDGQS 554 HSP 4 Score: 167.548 bits (423), Expect = 4.194e-38 Identity = 183/800 (22.88%), Postives = 362/800 (45.25%), Query Frame = 3
Query: 972 PDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGE---EFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYY-------PIEDMSKMALFDTTDIAQDACQ---IP-KPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKT------TPAIKPQTCS-SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIF-VGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQRQDECTMK---KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS--SGEF-YNVKTFTDLEKFNAKTISNEAC 3227
PD CN D++FVLD+S S++ + + + F ++K I +V V I+S L +K + K + ++ + G+T +NAL R+ + R GA I++TDG ++P + A +LK G V IG+G +V + +L ++S +++ +E++ K ++A C+ IP +P C+ + DI ++D S V + L F K + F++G + R + SF+N+ V ++ I+ + L + G T AL L S + + + + +++T+G S + T A L+++G + +IG+G + EL +++ P+ F DF S+ ++ +C+ P P C+ + DIIF +D S + + + + +QS +I + R+ ++ +SD + EF L++ F ++ AI ++ + AL R + + R ++ IV++ TD + +EA LK + +F +G+ + Y +++++ + + ++ ++ + C+ K + + K + D++ +D S S+G NFQ L FV + ++ ++ D +IG+ TF+S ++ + + + K + KIP G T +G A+ + +K R +V + +++TDG + + A K K G K+ ++G+G + +LN IAS + +F + F L+ A +S ++C
Sbjct: 236 PD--CNVSMADILFVLDSSGSVSNPDFVRMITFVKEMVKGFDISNSTVRVAVSIFSGGTVEKFHLDKYFDKAALLKAIGQISRVTGSTNTANALTRARTVSFQPPHGDRSGAPNIAIVITDGNSDNPIQTTDA-ALQLKNTGTAVFSIGVGSNVRQSELESIASDKSHIFTVTNFKALENIKK-------NLAIKTCEAQVIPTQPAITCNQSMADIVFLIDSSGSVGSSNFQKMLQFVKNMVNGFDVGKDTVRIGVASFSNKPVQHFALNTYFNRSQIIDAISKLPYYQGSTNTASALGLLRSSVFTAASGDRPTAPNIAIVITDGQSNDPSSTAAQAKALRQTGTILFSIGVGSGVNARELNNMASDPDKDHVFTVSDFSAFGSIQKELAIKACEAQKYTPLPPTKSPVVCNRTMADIIFALDTSASVDEHSYKLMLTFVHRMVQSFDIGKDDVRVGLVTFSDNSVLEFHLNKYFNASELDKAISNVKYKAGSTNTADALRYIRTISLLPQNGNRPNIPNIVIVLTDGKSNVPASTTAEAMKLK--KLATVFSIGIGSQAFYRELKDISTDPDADHVFTVSNFKALEKIRATLAVRTCEVGKNKTEPEIRKCPAQADIVFILDSSGSVGSANFQKMLRFVETVSKSFNIGPDEVQIGVDTFDSGSRTEFNMNKHKDQQSLLKAVGKIPTRSGMTHMGEALKRLRETSMSKAAGMRSNVSHIAIMITDGRPNGRLNVVQEAKKVKDSGTKLFTIGIGPSVDYNQLNVIASKPTKQFVFKADNFDSLKSI-ASMLSRQSC 1022
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A2C9JSZ0 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106066853 PE=4 SV=1) HSP 1 Score: 276.944 bits (707), Expect = 2.927e-71 Identity = 296/1168 (25.34%), Postives = 531/1168 (45.46%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKM---------ALFDT--------TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIK--------------PQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKI--IFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF--------------KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQI-SAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIP--------------QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSAR-VLFKSDT--RKNATKTVIIIIDG--EITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLN---STRKEVPKVIVLVTDGVST 4166
C +LDL+FVLDAS+S+ ++ F + + ID +VGV+IYSD + L + K ++ + + G+T ++AL+ + G R ++VTDG N + + EAE+ + GI + IG+GL+ K +L+ ++S E + ++D +++ AL T T PK + C+ AKLD+ ++D S+ V + F K F+ ++ R +V +++ V+ + D+ ++ + + G T AL RS + N R + V++T+G+S + +T+ A + G+ I IG+G D +EL +++ P N F QDF E + L K C T P ++ Q S G D++FV+D ST T ++ ++ + L +I R+ ++ YSD +F+L+ K D+Y AI + + AL R V R+DV + V+ TD + N + ++ EAE + +G+ I I +GL+ + + ++ +++ ++ + ++ + +C KQR C KLD++ +D STS+ NF++ DFV + + D+ D R+G+ ++ Y+ ++S + AD+ I+ IP+ G+T A+ +FT G R DV V VV+TDG ++I + A +A+ GI I ++G+G KEL+ IAS + V+ F++L K S P Q+ + + DL+FV+ +S + T F+ M DF+K +D +N RV ++ ++ +Y+ K + ++I+D+ AL + R V+F + R + +++ DG I R T EA + +GI ++ IG+ G T +L +AS D+ F V +F+ L+ + S LC I + EC+ + DL+ +DSS+S+ + L FV+ + DI++ +VG++T+N + +EF L+ K ++ V + ++ G+T+ EAL +N + + R +VP +++TDGVST
Sbjct: 472 CAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRAVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTK-ELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPTRTEPTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALK-TMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARADGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEQTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPAVRTEPTPPPTTPKQRV-SCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNSYAKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLT-DTKELDGIASKPVDENRFAVQDFSELRDLRHKVFSALCSTSPPVKNEPTPPPTTPKQRVSCAGSKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSR-RTVPEAEQARAEGIHIYAIGI-GLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCGAIPTASSECALARADLVIVIDSSTSVTESNFDKILSFVKDFLSKTDIDSGNVRVGLITYNNEVSQEFYLNSAFTKREILELVDDVKYNFGSTNTAEALEIMRNVMFSPEKGDRADVPNFAIVLTDGVST 1632 HSP 2 Score: 263.848 bits (673), Expect = 4.226e-67 Identity = 301/1199 (25.10%), Postives = 531/1199 (44.29%), Query Frame = 3
Query: 963 ISTPDQN--------CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKM---------ALFDT--------TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKT----------TPAIKPQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKI--IFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF------------------KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQI-SAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIP------------------QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGF-----KEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDG--EITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKE-------------CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL---NSTRKEVPKVIVLVTDGVS 4163
I+ P++N C +LDL+FVLDAS+S+ ++ F + + ID +VGV+IYSD + L + K ++ + + G+T ++AL+ + G R ++VTDG N + + EAE+ + GI + IG+GL+ K +L+ ++S E + +++ +++ AL T T PK + C+ AKLD+ ++D S+ V + F K F+ ++ R +V +++ V+ + D+ ++ + + G T AL RS + N R + V++T+G+S + +T+ A + G+ I IG+G D +EL +++ P N F QDF E + L K C T P Q S G D++FV+D ST T ++ ++ + L +I R+ ++ YSD +F+L+ K D+Y AI + + AL R V R+DV + V+ TD + N + ++ EAE + +G+ I I +GL+ + + ++ +++ ++ + ++ + +C KQR C KLD++ +D STS+ NF++ DFV + + D+ R+G+ ++ Y+ +++ + AD+ I+ IP+ G+T A+ +FT G R DV V VV+TDG ++I + A +A+ GI I ++G+G KEL+ IAS + V+ FT+L K S +P Q+ + A DL+FV+ +S + T F+ M DF+K +D +N RV ++ ++ +Y+ K D N I STN K + S + R + +++ DG I R T EA + +GI ++ IG+ G T +L +AS D+ F V +F+ L+ + S LC + E C+ +LDL+F LD+S+S+ ++ DFV+ + D DI+ + +VGVV ++ +F L+ + +K + +A+ I + G+T+ +AL+ + + N R +V V V+VTDGVS
Sbjct: 35 IAYPNKNNPTRKPKGCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVNNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTK-ELDGIASKPVDENRFAVQEFTELRDLRHKVFSALCSTLPPVSTEPTPPPTRPPITPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALK-TMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTKPTPPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRAVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPTRTEPTPPPTRPPTTPKQRV-SCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARADGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEQTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSR-RTVPEAEQARAEGIHIYAIGI-GLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPAVRTEPTPPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNSYAKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVS 1226 HSP 3 Score: 231.876 bits (590), Expect = 2.561e-57 Identity = 258/1022 (25.24%), Postives = 463/1022 (45.30%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFDTTDIAQDACQIP-----------------KPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIK----------PQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKI--IFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDE-------------CTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQI-SAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN----RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSAR-VLFKSDT--RKNATKTVIIIIDGEITDRNE-TFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP---DKALFVDNFSNLQRYNAYISSELC 3785
C +LDL+FVLDAS+S+ ++ F + + ID +VGV+IYSD + L + K ++ + + G+T ++AL+ + G R ++VTDG N + + EAE+ + GI + IG+GL+ K +L+ ++S E + ++D +++ + +P K + C+ AKLD+ ++D S+ V + F K F+ ++ + R +V +++ V+ + + Y D+ ++ + + G T AL RS + N R + V++T+G+S + +T+ A + G+ I IG+G D +EL +++ P N F QDF E + L K C T PA++ Q S G D++FV+D ST T ++ ++ + L +I R+ ++ YSD +F+L+ K D+Y AI + + AL R V R+DV + V+ TD + N + ++ EAE + +G+ I I +GL+ + + ++ +++ +++ + ++ + +C ++E C KLD++ +D STS+ NF++ DFV + + D+ D R+G+ ++ Y+ +++ + AD+ I+ IP+ G+T A+ +FT G R DV V VV+TDG ++I + A +A+ GI I ++G+G KEL+ IAS + V+ FT+L K S IP + C A DL+ V+ SS + T F ++ F+K T +D N RV ++ +N + Y++S F + + ++D+ + +AL R V+F + R + I++ DG T E T A L DGI +FTIG+ G + +L+ +A+P + VDNF L+ + I + C
Sbjct: 685 CAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARADGIHIYAIGIGLTDTK-ELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEQTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQL-NTYSKKADVYNAIDDIPYRYGSTNTADALK-TMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPAVRTEPTPPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNSYAKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTKELDGIASKPVDENRFAVQDFSELRDLRHKVFSALCSTSPPVKNEPTPPPTTPKQRVSCAGSKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLT-DTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCGAIPTASSECALARADLVIVIDSSTSVTESNFDKILSFVKDFLSKTDIDSGNVRVGLITYNNEVSQEFYLNSAFTK--REILELVDDVKYNFGSTNTAEALEIMRNVMFSPEKGDRADVPNFAIVLTDGVSTVNAERTVPNARLAHDDGIHIFTIGV-GLSDTRELEGIATPPASQNSFKVDNFDALEYVDDLIFASAC 1698 HSP 4 Score: 106.686 bits (265), Expect = 4.976e-19 Identity = 214/1025 (20.88%), Postives = 392/1025 (38.24%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY---DDILKKVESLKH---------VGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTN---GLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKI-----------------LSTFPNPFL---RQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRAR-QVF--KIDYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFY--------ENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQD-EC-----------------------TMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPK---VLVVLTDGANDIGESPQISAVKAKL-GGIKIISVGVGQQ-----------------ISEKELNDIASSGEFYN--VKTFTDLEKFNAKTISNEA----------CQIPQ--KPTICKAAGDLIFVMGSSAN----KTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN-----RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP----DKALFVDNFSNLQRYNAYISSELCDV-----------------------------IKGTNKE-CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLN---STRKEVPKVIVLVTDGVSTGGLSPEL 4187
DI ++D S QL W+ L + F + + R ++F + + E + Y D+++ + + + G F + ++ AS +++N V++LT L+ ER + AA L++ GVDI +IG + E +I L PN FL R + + + C + GDIIF++D S E + +++ +T++ L+I R+ II +SD + EF L+ T+ D+ I+++ AL R R ++F + R+D ++V+ TD N +++L EA +K G I+ V + Y ENL ++ I ++ C+ R + EC DV++ +D STS+GP F+ + ++ L++ ++G+ ++S + + + F M + I I + G + + A +F D P + +LTDG ++ + + V+ + GI I + V Q I E E N + S +F V + + + N N C+ P+ IC + + T F+ + F+KS K N R+ I+++ R I+ K + SS+ + N + L S+ VLF+ RK V+I+ EI N T + N LK G K IG+ S+ L +A D +D L+ ++ +C+ G N E D++F +DSS S+G + ++ ++V+ ++ +I T+VG+ TF+ ++ E L+ + +K A+ +AV + ++ G T+ L+ + + + R VP +V++TDG+S ++PE
Sbjct: 1799 DIIFMMDSSVNNQLFGWMKSFL--RDFTNIVNVDDGEWRIGAMTF-DRSTRNEFHLNRYPFNDEVVGALNRVNNRPSSSDPDVAGAFDYVRNSMFTASNGDRPRARN---YVIILTGKEKSLNTER--SYAAANRLKDYGVDIFSIGFNFRNSTELDEISSKPVEDFRYLVFSEDQLKEIPNTFLYNIRNAPPQTPRPQPATTPRPTRPPAPPYTGKCDAKGDIIFILDSSGSVGEENFRMMKNFTYSTVKDLSIDNGYFRVGIITFSDSSRIEFHLNGYNTREDLIEQIEKIPYTYGYTHTAEALRRVRTEMFTQRNGDRSDAPNLLVIVTDGHSNINHEETLPEARRIKETGTTIVTVAVGVKGNYELRQMTSEPVDENLLEVTDFSALDQITRLIVAPVCTDENRCERNPCRNNGECVDGLRSYMCRCLPGFYGTNCEKACGPPADVVLILDSSTSVGPTTFEDIKKYAQAMISELNIMTCNIQVGVIKYSSAAMIQFNLGTIFTEEGMYRAIGDISYTPGRSNMAEAFRVVRTQMFNNRNGDRPNARNIAYLLTDGTVEVNKDITSNEVELSIDAGIHIFPLAVAIQDRSEIEYIAQVQNIPFLIIEDEKNFMEQSDQFLRAVVDSPSADDSCNPNPCRNNGQCVDEPFGFTCKCPEGFAGEICDKVCSSSVDVVFVVDASKYNTGPDFRTIKRFLKSNVKNLAFKSGNIRIGIVEYGAHPRLRLELIEGDNKRTINSVISSLRRVDGDPN-LEQAIKLVSSNVLFRGGDRKEVPNYVVILAR-EIGGEN-TVQAINELKSKGTKFIGIGMGLSKSDEKLLEIAVSSALEDVHHLLDGPQRLEGIALDFATLVCEAKDQCVNSPCQNGGTCENGEKTYACRCPPGFAGKNCERACDDSADIVFLIDSSGSVGFENFRHIKEYVQNVIDHFNIGPTATQVGIATFSQSSKAEIFLNSYKDKRALKAAVSGLKYEYGNTNTASGLKLSRTYMFSPAKGNRPNVPDFLVVITDGLSN--VNPEF 2810 HSP 5 Score: 99.7525 bits (247), Expect = 5.706e-17 Identity = 129/561 (22.99%), Postives = 245/561 (43.67%), Query Frame = 3
Query: 2736 GPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIG-NATDYLFTKG-REDVPKVLVVLTDGANDIGESPQISAV-KAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTD----LEKF---------------------NAKTISN----EACQIPQK------PTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETF--EEANLLKKDGIKVFTIGLTGKTSES-DLKSVAS-PDKA----------LF------VDNFSNLQRYN------------AYISSELCDVIKG-----TNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVP---KVIVLVTDGVS 4163
GP+ F+ F+ V+NL + RIGI + + L + + + I+ + ++G+ + AI +++ LF G R++VP +V+L A +IG + A+ + K G K I +G+G S+++L +IA S +V D LE N T N AC+ P C + D++F++ SS + E F+ + ++++++ + T+V I F++ + +++S +D + ++++ + L +R S R N +++I DG +++ N F EA L +K GI V +G+ K ++ +LK +AS P A LF VDN Q A S C +G +C + D++F LDSS+S+GP ++ L +V +V+D+ + + +V ++T+ + F +++ +++A+K ++ G+T+ LR+ EL +S+ + P + +L+TDG S
Sbjct: 2480 GPD-FRTIKRFLKSNVKNLAFKSGNIRIGIVEYGAHPRLRLELIEGDNKRTINSVISSLRRVDGDPNLEQAIKLVSSNVLFRGGDRKEVPNYVVIL---AREIGGENTVQAINELKSKGTKFIGIGMGLSKSDEKLLEIAVSSALEDVHHLLDGPQRLEGIALDFATLVCEAKDQCVNSPCQNGGTCENGEKTYACRCPPGFAGKNCERACDDSADIVFLIDSSGSVGFENFRHIKEYVQNVIDHFNIGPTATQVGIATFSQSSKAEIFLNS--YKDKRALKAAVSGLKYEYGNTNTASGLKLSRTYMFSPAKGNRPNVPDFLVVITDG-LSNVNPEFTIPEAELARKQGIHVIAVGINLKDNDDWELKGIASEPVNANVFRVNAFDKLFDITDKIVDNTCRDQSVCDTNPCKNDGNCIASAGSYTCQCQQGYLGPNCEYDCQNNK-DIVFILDSSASVGPSNFELMLKYVSEVVKDLTLYGQDHRFSLVIYSTEVKTIFSFNRYNSPKDILNAIKTTVYMPGSTNTAGGLRQAL-ELFSSSFGDRPTADNIAILLTDGQS 3031 HSP 6 Score: 80.4925 bits (197), Expect = 3.504e-11 Identity = 99/397 (24.94%), Postives = 176/397 (44.33%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT--KG-REDVPKVLVVLTDGANDIGESPQISAVKAKLG---GIKIISVGVGQQISEK-ELNDIAS---SGEFYNVKTFTDLEKFNAKTISNEA-------------------------CQIPQ---KPTI---CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVA----------ILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEI-TDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF 3728
D++ ID S S+G ENF+ ++V ++ + ++ T++GI TF+ + ++S + ++ ++ + + GNT + + Y+F+ KG R +VP LVV+TDG +++ +P+ + +A+L GI +I+VG+ + ++ EL IAS + + V F L K + N CQ Q P C+ D++F++ SSA+ F+ M ++ + K + ++ R + I FNR N +D N + M STN L A LF S R A I++ DG+ + +T A LK + KV TIG+ + + ++K++AS K +F
Sbjct: 2691 DIVFLIDSSGSVGFENFRHIKEYVQNVIDHFNIGPTATQVGIATFSQSSKAEIFLNSYKDKRALKAAVSGLKYEYGNTNTASGLKLSRTYMFSPAKGNRPNVPDFLVVITDGLSNV--NPEFTIPEAELARKQGIHVIAVGINLKDNDDWELKGIASEPVNANVFRVNAFDKLFDITDKIVDNTCRDQSVCDTNPCKNDGNCIASAGSYTCQCQQGYLGPNCEYDCQNNKDIVFILDSSASVGPSNFELMLKYVSEVVKDLTLYGQDHRFSLVIYSTEVKTIFSFNRYNS-----PKDILNAIKTTVYMPGSTN---TAGGLRQALELFSSSFGDRPTADNIAILLTDGQSNVNYLDTIPAATDLKAEKNKVITIGIALQNLD-EIKAIASSPKDVF 3076
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Match: A0A2C9JSY7 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106066853 PE=4 SV=1) HSP 1 Score: 276.174 bits (705), Expect = 5.613e-71 Identity = 296/1169 (25.32%), Postives = 533/1169 (45.59%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKM---------ALFDT--------TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIK--------------PQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKI--IFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF--------------KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQI-SAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIP--------------QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSAR-VLFKSDT--RKNATKTVIIIIDG--EITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLN---STRKEVPKVIVLVTDGVST 4166
C +LDL+FVLDAS+S+ ++ F + + ID +VGV+IYSD + L + K ++ + + G+T ++AL+ + G R ++VTDG N + + EAE+ + GI + IG+GL+ K +L+ ++S E + ++D +++ AL T T PK + C+ AKLD+ ++D S+ V + F K F+ ++ R +V +++ V+ + + Y D+ ++ + + G T AL R+ + N R + V++T+G+S + +T+ A + G+ I IG+G D +EL +++ P N F QDF E + L K C T P ++ Q S G D++FV+D ST T ++ ++ + L +I R+ ++ YSD +F+L+ K D+Y AI + + AL R V R+DV + V+ TD + N + ++ EAE + +G+ I I +GL+ + + ++ +++ ++ + ++ + +C KQR C KLD++ +D STS+ NF++ DFV + + D+ D R+G+ ++ Y+ ++S + AD+ I+ IP+ G+T A+ +FT G R DV V VV+TDG ++I + A +A+ GI I ++G+G KEL+ IAS + V+ F++L K S P Q+ + + DL+FV+ +S + T F+ M DF+K +D +N RV ++ ++ +Y+ K + ++I+D+ AL + R V+F + R + +++ DG I R T EA + +GI ++ IG+ G T +L +AS D+ F V +F+ L+ + S LC I + EC+ + DL+ +DSS+S+ + L FV+ + DI++ +VG++T+N + +EF L+ K ++ V + ++ G+T+ EAL +N + + R +VP +++TDGVST
Sbjct: 685 CAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARADGIHIYAIGIGLTDTK-ELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEPTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQL-NTYSKKADVYNAIDDIPYRYGSTNTADALK-TMRAVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEQTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPAVRTEPTPPPTTPKQRV-SCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNSYAKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLT-DTKELDGIASKPVDENRFAVQDFSELRDLRHKVFSALCSTSPPVKNEPTPPPTTPKQRVSCAGSKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSR-RTVPEAEQARAEGIHIYAIGI-GLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCGAIPTASSECALARADLVIVIDSSTSVTESNFDKILSFVKDFLSKTDIDSGNVRVGLITYNNEVSQEFYLNSAFTKREILELVDDVKYNFGSTNTAEALEIMRNVMFSPEKGDRADVPNFAIVLTDGVST 1845 HSP 2 Score: 256.529 bits (654), Expect = 6.728e-65 Identity = 299/1203 (24.85%), Postives = 536/1203 (44.56%), Query Frame = 3
Query: 963 ISTPDQN--------CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKM---------ALFDT--------TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKT----------TPAIKPQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKI--IFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF------------------KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQI-SAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQI------------------PQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSAR-VLFKSDT--RKNATKTVIIIIDG--EITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKE-----------------CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL---NSTRKEVPKVIVLVTDGVS 4163
I+ P++N C +LDL+FVLDAS+S+ ++ F + + ID +VGV+IYSD + L + K ++ + + G+T ++AL+ + G R ++VTDG N + + EAE+ + GI + IG+GL+ K +L+ ++S E + +++ +++ AL T T PK + C+ AKLD+ ++D S+ V + F K F+ ++ R +V +++ V+ + D+ ++ + + G T AL RS + N R + V++T+G+S + +T+ A + G+ I IG+G D +EL +++ P N F QDF E + L K C T P Q S G D++FV+D ST T ++ ++ + L +I R+ ++ YSD +F+L+ K D+Y AI + + AL R V R+DV + V+ TD + N + ++ EAE + +G+ I I +GL+ + + ++ +++ ++ + ++ + +C KQR C KLD++ +D STS+ NF++ DFV + + D+ R+G+ ++ Y+ +++ + AD+ I+ IP+ G+T A+ +FT G R DV V VV+TDG ++I + A +A+ GI I ++G+G KEL+ IAS + V+ FT+L K S + Q+ + A DL+FV+ +S + T F+ M DF+K +D N RV ++ ++ +Y+ K + ++I+D+ AL + R V+F + R + +++ DG I R T EA + +GI ++ IG+ G T +L +AS D+ F V +F+ L+ + S LC + E C+ +LDL+F LD+S+S+ ++ DFV+ + D DI+ + +VGVV ++ +F L+ +++K + +A+ I + G+T+ +AL+ ++ + N R +V V V+VTDGVS
Sbjct: 35 IAYPNKNNPTRKPKGCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVNNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTK-ELDGIASKPVDENRFAVQEFTELRDLRHKVFSALCSTLPPVSTEPTPPPTRPPITPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALK-TMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTKPTPPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRAVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPTRTEPTPPPTRPPTTPKQRV-SCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYAKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARADGIHIYAIGIGLT-DTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEPTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRAVMFTAGNGDRSDVDNVAVVVTDGVSNINSR-RTVPEAEQARAEGIHIYAIGI-GLTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEQTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRSVMFTAGNGDRSDVDNVAVVVTDGVS 1230 HSP 3 Score: 233.032 bits (593), Expect = 1.395e-57 Identity = 258/1022 (25.24%), Postives = 463/1022 (45.30%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFDTTDIAQDACQIP-----------------KPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR-----LVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIK----------PQTCSSNG---DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKI--IFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDE-------------CTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQI-SAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN----RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSAR-VLFKSDT--RKNATKTVIIIIDGEITDRNE-TFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP---DKALFVDNFSNLQRYNAYISSELC 3785
C +LDL+FVLDAS+S+ ++ F + + ID +VGV+IYSD + L + K ++ + + G+T ++AL+ + G R ++VTDG N + + EAE+ + GI + IG+GL+ K +L+ ++S E + ++D +++ + +P K + C+ AKLD+ ++D S+ V + F K F+ ++ + R +V +++ V+ + + Y D+ ++ + + G T AL RS + N R + V++T+G+S + +T+ A + G+ I IG+G D +EL +++ P N F QDF E + L K C T PA++ Q S G D++FV+D ST T ++ ++ + L +I R+ ++ YSD +F+L+ K D+Y AI + + AL R V R+DV + V+ TD + N + ++ EAE + +G+ I I +GL+ + + ++ +++ +++ + ++ + +C ++E C KLD++ +D STS+ NF++ DFV + + D+ D R+G+ ++ Y+ +++ + AD+ I+ IP+ G+T A+ +FT G R DV V VV+TDG ++I + A +A+ GI I ++G+G KEL+ IAS + V+ FT+L K S IP + C A DL+ V+ SS + T F ++ F+K T +D N RV ++ +N + Y++S F + + ++D+ + +AL R V+F + R + I++ DG T E T A L DGI +FTIG+ G + +L+ +A+P + VDNF L+ + I + C
Sbjct: 898 CAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGGNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRAVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTK-ELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPPVRTEQTPPPTRPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQL-NTYSKKADVYNAIDDIPYRYGSTNTADALK-TMRSVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIG-LTDTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCSTLPAVRTEPTPPPTTPKQRVSCAGAKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNSYAKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLTDTKELDGIASKPVDENRFAVQDFSELRDLRHKVFSALCSTSPPVKNEPTPPPTTPKQRVSCAGSKLDLVFVLDASTSVTEPNFELMKDFVKDFLLDADIDGDNVRVGVVIYSDADYVQFQLNTYSKKADVYNAIDDIPYRYGSTNTADALKTMRGVMFTAGNGDRSDVDNVAVVVTDGVSNINSRRTVPEAEQARAEGIHIYAIGIGLT-DTKELDGIASKPVDENRFAVQDFTELRDLRHKVFSALCGAIPTASSECALARADLVIVIDSSTSVTESNFDKILSFVKDFLSKTDIDSGNVRVGLITYNNEVSQEFYLNSAFTK--REILELVDDVKYNFGSTNTAEALEIMRNVMFSPEKGDRADVPNFAIVLTDGVSTVNAERTVPNARLAHDDGIHIFTIGV-GLSDTRELEGIATPPASQNSFKVDNFDALEYVDDLIFASAC 1911 HSP 4 Score: 106.301 bits (264), Expect = 6.159e-19 Identity = 214/1025 (20.88%), Postives = 392/1025 (38.24%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY---DDILKKVESLKH---------VGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTN---GLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKI-----------------LSTFPNPFL---RQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRAR-QVF--KIDYRADVAKIVVLFTDKLPT-NQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFY--------ENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQD-ECT-----------------------MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPK---VLVVLTDGANDIGESPQISAVKAKL-GGIKIISVGVGQQ-----------------ISEKELNDIASSGEFYN--VKTFTDLEKFNAKTISNEA----------CQIPQ--KPTICKAAGDLIFVMGSSAN----KTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN-----RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP----DKALFVDNFSNLQRYNAYISSELCDV-----------------------------IKGTNKE-CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLN---STRKEVPKVIVLVTDGVSTGGLSPEL 4187
DI ++D S QL W+ L + F + + R ++F + + E + Y D+++ + + + G F + ++ AS +++N V++LT L+ ER + AA L++ GVDI +IG + E +I L PN FL R + + + C + GDIIF++D S E + +++ +T++ L+I R+ II +SD + EF L+ T+ D+ I+++ AL R R ++F + R+D ++V+ TD N +++L EA +K G I+ V + Y ENL ++ I ++ C+ R + EC DV++ +D STS+GP F+ + ++ L++ ++G+ ++S + + + F M + I I + G + + A +F D P + +LTDG ++ + + V+ + GI I + V Q I E E N + S +F V + + + N N C+ P+ IC + + T F+ + F+KS K N R+ I+++ R I+ K + SS+ + N + L S+ VLF+ RK V+I+ EI N T + N LK G K IG+ S+ L +A D +D L+ ++ +C+ G N E D++F +DSS S+G + ++ ++V+ ++ +I T+VG+ TF+ ++ E L+ + +K A+ +AV + ++ G T+ L+ + + + R VP +V++TDG+S ++PE
Sbjct: 2012 DIIFMMDSSVNNQLFGWMKSFL--RDFTNIVNVDDGEWRIGAMTF-DRSTRNEFHLNRYPFNDEVVGALNRVNNRPSSSDPDVAGAFDYVRNSMFTASNGDRPRARN---YVIILTGKEKSLNTER--SYAAANRLKDYGVDIFSIGFNFRNSTELDEISSKPVEDFRYLVFSEDQLKEIPNTFLYNIRNAPPQTPRPQPATTPRPTRPPAPPYTGKCDAKGDIIFILDSSGSVGEENFRMMKNFTYSTVKDLSIDNGYFRVGIITFSDSSRIEFHLNGYNTREDLIEQIEKIPYTYGYTHTAEALRRVRTEMFTQRNGDRSDAPNLLVIVTDGHSNINHEETLPEARRIKETGTTIVTVAVGVKGNYELRQMTSEPVDENLLEVTDFSALDQITRLIVAPVCTDENRCERNPCRNNGECVDGLRSYMCRCLPGFYGTNCEKACGPPADVVLILDSSTSVGPTTFEDIKKYAQAMISELNIMTCNIQVGVIKYSSAAMIQFNLGTIFTEEGMYRAIGDISYTPGRSNMAEAFRVVRTQMFNNRNGDRPNARNIAYLLTDGTVEVNKDITSNEVELSIDAGIHIFPLAVAIQDRSEIEYIAQVQNIPFLIIEDEKNFMEQSDQFLRAVVDSPSADDSCNPNPCRNNGQCVDEPFGFTCKCPEGFAGEICDKVCSSSVDVVFVVDASKYNTGPDFRTIKRFLKSNVKNLAFKSGNIRIGIVEYGAHPRLRLELIEGDNKRTINSVISSLRRVDGDPN-LEQAIKLVSSNVLFRGGDRKEVPNYVVILAR-EIGGEN-TVQAINELKSKGTKFIGIGMGLSKSDEKLLEIAVSSALEDVHHLLDGPQRLEGIALDFATLVCEAKDQCVNSPCQNGGTCENGEKTYACRCPPGFAGKNCERACDDSADIVFLIDSSGSVGFENFRHIKEYVQNVIDHFNIGPTATQVGIATFSQSSKAEIFLNSYKDKRALKAAVSGLKYEYGNTNTASGLKLSRTYMFSPAKGNRPNVPDFLVVITDGLSN--VNPEF 3023 HSP 5 Score: 99.3673 bits (246), Expect = 6.940e-17 Identity = 129/561 (22.99%), Postives = 245/561 (43.67%), Query Frame = 3
Query: 2736 GPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIG-NATDYLFTKG-REDVPKVLVVLTDGANDIGESPQISAV-KAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTD----LEKF---------------------NAKTISN----EACQIPQK------PTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGN----YIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETF--EEANLLKKDGIKVFTIGLTGKTSES-DLKSVAS-PDKA----------LF------VDNFSNLQRYN------------AYISSELCDVIKG-----TNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVP---KVIVLVTDGVS 4163
GP+ F+ F+ V+NL + RIGI + + L + + + I+ + ++G+ + AI +++ LF G R++VP +V+L A +IG + A+ + K G K I +G+G S+++L +IA S +V D LE N T N AC+ P C + D++F++ SS + E F+ + ++++++ + T+V I F++ + +++S +D + ++++ + L +R S R N +++I DG +++ N F EA L +K GI V +G+ K ++ +LK +AS P A LF VDN Q A S C +G +C + D++F LDSS+S+GP ++ L +V +V+D+ + + +V ++T+ + F +++ +++A+K ++ G+T+ LR+ EL +S+ + P + +L+TDG S
Sbjct: 2693 GPD-FRTIKRFLKSNVKNLAFKSGNIRIGIVEYGAHPRLRLELIEGDNKRTINSVISSLRRVDGDPNLEQAIKLVSSNVLFRGGDRKEVPNYVVIL---AREIGGENTVQAINELKSKGTKFIGIGMGLSKSDEKLLEIAVSSALEDVHHLLDGPQRLEGIALDFATLVCEAKDQCVNSPCQNGGTCENGEKTYACRCPPGFAGKNCERACDDSADIVFLIDSSGSVGFENFRHIKEYVQNVIDHFNIGPTATQVGIATFSQSSKAEIFLNS--YKDKRALKAAVSGLKYEYGNTNTASGLKLSRTYMFSPAKGNRPNVPDFLVVITDG-LSNVNPEFTIPEAELARKQGIHVIAVGINLKDNDDWELKGIASEPVNANVFRVNAFDKLFDITDKIVDNTCRDQSVCDTNPCKNDGNCIASAGSYTCQCQQGYLGPNCEYDCQNNK-DIVFILDSSASVGPSNFELMLKYVSEVVKDLTLYGQDHRFSLVIYSTEVKTIFSFNRYNSPKDILNAIKTTVYMPGSTNTAGGLRQAL-ELFSSSFGDRPTADNIAILLTDGQS 3244 HSP 6 Score: 80.8777 bits (198), Expect = 3.503e-11 Identity = 99/397 (24.94%), Postives = 176/397 (44.33%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT--KG-REDVPKVLVVLTDGANDIGESPQISAVKAKLG---GIKIISVGVGQQISEK-ELNDIAS---SGEFYNVKTFTDLEKFNAKTISNEA-------------------------CQIPQ---KPTI---CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVA----------ILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEI-TDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF 3728
D++ ID S S+G ENF+ ++V ++ + ++ T++GI TF+ + ++S + ++ ++ + + GNT + + Y+F+ KG R +VP LVV+TDG +++ +P+ + +A+L GI +I+VG+ + ++ EL IAS + + V F L K + N CQ Q P C+ D++F++ SSA+ F+ M ++ + K + ++ R + I FNR N +D N + M STN L A LF S R A I++ DG+ + +T A LK + KV TIG+ + + ++K++AS K +F
Sbjct: 2904 DIVFLIDSSGSVGFENFRHIKEYVQNVIDHFNIGPTATQVGIATFSQSSKAEIFLNSYKDKRALKAAVSGLKYEYGNTNTASGLKLSRTYMFSPAKGNRPNVPDFLVVITDGLSNV--NPEFTIPEAELARKQGIHVIAVGINLKDNDDWELKGIASEPVNANVFRVNAFDKLFDITDKIVDNTCRDQSVCDTNPCKNDGNCIASAGSYTCQCQQGYLGPNCEYDCQNNKDIVFILDSSASVGPSNFELMLKYVSEVVKDLTLYGQDHRFSLVIYSTEVKTIFSFNRYNS-----PKDILNAIKTTVYMPGSTN---TAGGLRQALELFSSSFGDRPTADNIAILLTDGQSNVNYLDTIPAATDLKAEKNKVITIGIALQNLD-EIKAIASSPKDVF 3289
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 222.631 bits (566), Expect = 4.307e-58 Identity = 269/1153 (23.33%), Postives = 507/1153 (43.97%), Query Frame = 3
Query: 945 TSNYIEISTPDQNCNNQQL-DLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNA----LRRS-GEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST---ETYYPIEDMSKMALFDTTDIA-----------QDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKS------KNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN-----PFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIK--QLRSNGNGGDLNTALNRAR-QVFKIDYRADVAKIVVLFTDKLPTNQKDS--LSEAEILKGDGVKIIFVGLSKNSPYNFYENL--------------AXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK-----GREDVPKVLVVLTDGANDIGESPQ---ISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTF---TDLEKFNAKTISNE-----ACQIPQ----KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIII-DGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNEL--LNSTRKEVPKVIVLVT 4151
+S Y+ S C + L D++F++D S S+ Q + F ++ S + ++ ++G++ YS + L +K EI ++S L G T LR EE S+ ++G + +++TDG E A++LK+ GI + IG+ + + DQL +++S + Y + D + + + I + Q+P C A L DI +VD SS + +++ F FI+ E+ + R ++V F+N + D ++L+KV+ L + G T KALN ++ ++ N R+ V++T+G S + ++T A +L+ GV I TIG+G+ + EL+ ++ P+ F+ +++ + + C + + D+ +VD S+ IR ++ L++ +R+++I + E EF L+ T+R+ I+ QLRS+G +L A++ R ++ + + +A+ + + + D+ L LK +GVKI+ V SK F L A ++ + + D D + + D++ +D S ++ NF + DF++R+++ LD+ D RIG+ + +E + + D+ + I +P+ G A+ A + +F+K V ++ V+T+ GESP A K + G+++ ++G+ Q + +L IAS YNV +F T +E+F KT+ N +I Q + + D+ F++ S + F+ M F+ + + + RV ++KF ++ + D + E ++ ++ G G+ALT R F TR + ++I+I DGE TD E + A LK G+ ++ +G+ L+ +SP K FV+N+ +L+ I +++C + C D++F +D S SIG + + FV V + I + +VG++ F T+ R EF L+ + +K M+ AV + + + +IG+AL E R+ PK ++L+T
Sbjct: 14 SSCYLSSSAQKTVCTQEALADIVFLVDGSWSIGTENFQRMREFLFTLVDSFDVGLEKVRIGLVQYSTTVHTEFLLNGYNSKQEILDYISNLPYRGGGTKTGLGLDFLLRYHFKEEAGSRAKKGTPQIAVVITDGQSQDNV---EPYAQELKQKGIILYAIGIKDADM-DQLKEIASQPHDQHIYSVSDFTALQGISQSFITVLCSTVEEEAKRQVTQVPD----CRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNFIKDRYFTQAGRIQARANVPRIAVVITDGDSTDDMKT--PAQELRREGVLIFTIGVGE-VNMAELQSIANKPHQRFVISFV--NYEALVNAASNMMNTVCISMEDKQRALAPKYSDVFVLVDSSSQQIAN----IRKFLIQLATQLDVGAKVNRMALIQFGGEVNVEFLLNAYNTRREAIALIQKFQLRSSGR-SNLGQAMDYVRTRLLNSESGSRIAQGFKQYLLVVGKGETDTAVLRATRALKAEGVKIVEVDTSKRVDLVFSGALPGSSGVPVLDKTVIAPGRTFFSATQDLTNIVQDVKDSINVPDVISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITE-----GESPDDVTSEASKLRRSGVRVYALGI-QHKNVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYFIEARKTRDTRVREILIVITDGESTD--EVKQPAAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDIC-----SEDACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILIT 1135 HSP 2 Score: 205.682 bits (522), Expect = 6.795e-53 Identity = 245/941 (26.04%), Postives = 416/941 (44.21%), Query Frame = 3
Query: 1500 DTTDIAQD---ACQIPKPKRI---C-SAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQ--DFXXXXXXXXXXXXXXC-----KTTPAIK----PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYR----ADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF-TKG-REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY---NVKTFTDLEKFNAKTISNEA--CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALT-SARVLFKSDTRK---NATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVS 4163
D T+I QD + +P + C SA++ DI IVD S + + F R I+ ++G + R +V + NE E + +D DIL+ ++ L + GG KAL A + K S+R + ++T G S + V + A L+ SGV + +G+ K + ++I S P ++ F + + + K C +T I Q C + DI F++D S + ++ +L L NI R+ ++ + T EF + + + I A+ + G G + AL R F I+ R V +I+++ TD T++ + A LK GV I VG+ + + E +S +EN + + K++I IC +D C D+I +D S S+G E F FVN V L + KD ++G+ F + ++S ++ M + +++ E N IG A+ + TKG R++ PK L+++T + + A++ K G+ I S+G+G+ S + N S + Y N+ L++ I N A CQ Q D++F++ S + E F +M FM + T V + R + ++ + K+ Y E ++I ++ T + AL S R K++ + + + +I DGE TD + A L GI VF IG+ G+ E +L ++AS K +F VDN+ L+ + IS LC+ + ECS DL+ +D S SI W T ++F+ R V + + + ++GV F+T +KEF L++ E A+ A+K I T+IG AL + +E S + + ++L+TDG S
Sbjct: 604 DLTNIVQDVKDSINVPDVISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEF-YLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGESPDDVTS--EASKLRRSGVRVYALGIQHKNVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYF-IEARKTRDTRVREILIVITDGESTDEVKQPAAA--LKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDICS-----EDACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITGAESKDDVNAPAKALRDK--GVTIYSIGIGEAKSLQLRNISGSPKQVYMERNLDALDLLDENLLLKICNSADDCQKTQ-------VADVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSPAGKLHALGINVFGIGV-GEAKEDELMTIASDPKNVFKVDNYEALKALHQNISKVLCN---NSKPECSKVAADLVILIDGSESIEDAPWNTMINFMMRFVDSLRVREDLFRIGVAQFSTVYKKEFYLNEHMEPAAIKKAIKDIKQMKDGTNIGNALYQV-DEFFQSAAGSRINSGISQNLLLITDGKS 1519 HSP 3 Score: 156.762 bits (395), Expect = 8.488e-38 Identity = 237/1017 (23.30%), Postives = 459/1017 (45.13%), Query Frame = 3
Query: 963 ISTPD-----QNCNNQQL-DLIFVLDASSSLA-----LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGE-----EFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD--QLNKMSSTETYYPIEDMSKMALFD--------TTDIAQDACQIPK--PKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS-----KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNFENADMQKEIN------KIPFIEGNTAIGWAIGNATDYLFTK---GRED--VPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN----RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKS--DTRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYIS--SELCDVIKGTNKECS 3818
I+ PD +C + Q+ D++F++DAS +++ L D F + +IK L + ++G+++Y + + L + NK +I +++ L G S AL+ + E E S+ G + ++T+G SP EA KL++ G+RV +G+ + Q+ + Y ++ +K+ + + + + +I + K+ C + + DI ++D S + ++ + F + F +G + R +V F + +E T D I V ++ +GG T +AL R ++++++ + + +++++T+G S + V+ Q A L+ GV I +G+ + L++ S+ F +++ K L ++ V C + A K DIIF+VD S E + I+ + T+ L I + + ++ ++ + TEF+L+ + K + A+ Q ++ ALN + F R + K ++L T ++ D + A+ L+ GV I +G+ + S K N++ +D E + IC++ D+C ++ DV+ +D STS+ E+F F+N +V N ++ ++ R F + VY N P + N + ++E+N K P G+T A+ + + F+K GR + VP+++ V+TDG A D + P A K GI + +GVG+ E EL IAS + + V + L+ + + IS C KP K A DL+ ++ S + + M +FM V ++ R+ + +F+ + Y++ + + +I+D+ +G IG AL F+S +R N+ ++ +++I DG+ D + + L+ I +F IG+ G+ S ++ +A S D+ +D+F L+ + S +C +G EC+
Sbjct: 616 INVPDVISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRD----FLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEG--ESPD-DVTSEASKLRRSGVRVYALGIQHKNVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFM-RPYFIEARKTRDTRVREILIVITDGESTDEVK--QPAAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDIC-SEDACKQMMA----DIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITG--AESKDDVNAPAKALRDKGVTIYSIGIGEAKSLQLRNISGSPKQVYMERNLDALDLLDENLLLKICNS----ADDCQKTQVADVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVR-----FCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAP--TGDTYTASALQYSIRF-FSKAEGGRAEDGVPQLMFVITDGEATDHWDLPS-PAGKLHALGINVFGIGVGEA-KEDELMTIASDPKNVFKVDNYEALKALH-QNISKVLCN-NSKPECSKVAADLVILIDGSESIEDAPWNTMINFMMRFVDSLRVREDLFRIGVAQFSTVYKKEFYLNEHMEP--AAIKKAIKDIKQMKDGTNIGNALYQVDEFFQSAAGSRINSGISQNLLLITDGKSND--NIYNATDRLRARNINMFVIGI-GQISMKQIEYIAGSQDRVFVLDSFDALKLNTTILEVISHICQ--QGEESECT 1592 HSP 4 Score: 114.005 bits (284), Expect = 7.824e-25 Identity = 169/757 (22.32%), Postives = 349/757 (46.10%), Query Frame = 3
Query: 2082 CKTTPAIKPQTCSSNG--DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN-RARQVFKIDYRADVAK----IVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMI---CDAFKQ---RQ----DECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF--NAKTISNEAC-QIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSL-TKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESS----IEDMTTSTNGVF-IGKALTSARVLF---KSDTR-KNATKTVIIIIDGEITDRNETFEEANLLKKDGIK------------VFTIGLTGKTSESDL-KSVASPDKALF--VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNEL----LNSTR-KEVPKVIVLVTDGVSTGGLSPE 4184
C + + + C+ DI+F+VD S E + +R + T + S ++ RI ++ YS TEF L+ +K++I I L G G L+ R FK + + K I V+ TD +Q + A+ LK G+ + +G+ K++ + + +AS+ I +++ + ++ I C ++ RQ +C D++ +D S+S+G +F F++ +Q L++ D R+ + F++ + ++P+ + ++ ++++++ + G+T G A+ D FT + R +VP++ VV+TDG D + + A + + G+ I ++GVG +++ EL IA+ V +F + E A + N C + K +FV+ + +S++ + F+ L T+L V K N R+A+++F G ++ F + N I+ ++G +G+A+ R +S +R K ++++ TD LK +G+K VF+ L G + L K+V +P + F + +N+ + + S + DVI + +C + Q+ D++F +D+S +I + DF+ R+++ +D+ ++ ++G+V + + + EF L F K ++ ++ + + G + +AL+ + ++ L S R + V ++ ++T+G S ++ E
Sbjct: 16 CYLSSSAQKTVCTQEALADIVFLVDGSWSIGTENFQRMREFLFTLVDSFDVGLEKVRIGLVQYSTTVHTEFLLNGYNSKQEILDYISNLPYRGGGTKTGLGLDFLLRYHFKEEAGSRAKKGTPQIAVVITDG--QSQDNVEPYAQELKQKGIILYAIGI-KDADMDQLKEIASQPHDQHIYSVSDFTALQGISQSFITVLCSTVEEEAKRQVTQVPDCRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNFIKDRYFTQAGRIQARANVPRIAVVITDG--DSTDDMKTPAQELRREGVLIFTIGVG-EVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTVCISMEDKQRALAPKYSDVFVL---VDSSSQQIANIRKFLIQLATQLDVGAKVN-RMALIQF--GGEVNVEFLLNAYNTRREAIALIQKFQLRSSGRSNLGQAMDYVRTRLLNSESGSRIAQGFKQYLLVVGKGETD-TAVLRATRALKAEGVKIVEVDTSKRVDLVFSGALPGSSGVPVLDKTVIAPGRTFFSATQDLTNIVQ-DVKDSINVPDVIS-VSGDCKSAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGESPDDVTSE 757 HSP 5 Score: 59.6918 bits (143), Expect = 2.908e-8 Identity = 54/191 (28.27%), Postives = 92/191 (48.17%), Query Frame = 3
Query: 120 LDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPT--SEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
+ Q C+ + DI ++D S SI +FQ I + L F I P RV + + T EF T++ ++ AV ++GG + GRAL + + E R T + + IL++++DG +TDE VK A ++ +GV+IY V + LL SS + + +++ + +V+ IC
Sbjct: 830 IKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTET-GRAL-TFMRPYFIEARKTRDTRVREILIVITDGESTDE--VKQPAAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDIC 1016 HSP 6 Score: 52.7582 bits (125), Expect = 3.489e-6 Identity = 38/180 (21.11%), Postives = 81/180 (45.00%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTL--YDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
D+ ++D S+SI +F I +N ++ + N R + Y +T + +F KH + +N A+ + GD+ AL ++ + R + +++ +++DG TD + A K+ G+ ++ + +G L+ S KNV ++ ++ +N+ V+C
Sbjct: 1214 DVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYT-ASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSPAGKLHALGINVFGIGVGEAKEDELMTIASDPKNVFKVDNYEALKALHQNISKVLC 1392
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: si:ch211-62a1.3 (si:ch211-62a1.3 [Source:ZFIN;Acc:ZDB-GENE-061207-41]) HSP 1 Score: 210.69 bits (535), Expect = 3.485e-54 Identity = 270/1135 (23.79%), Postives = 519/1135 (45.73%), Query Frame = 3
Query: 981 NCNNQQL-DLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALR-----RSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKD---QLNKMSSTETY----YPIEDMSKMALFDTTDIAQDAC----------QIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKS-KNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFX----XXXXXXXXXXXXXCKTTPAIKPQTCSSN---GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNG-GDLNTALNRARQVFKIDYRADVAKIVVLFTDKL-----PTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKL--GGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQ------IP-------QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF-NRGNYIDSGFKEDYKN-FESSIEDMTTSTNGVFIGKALTSARVLFKS--DTRKNATKTVIIII-DGEITDRNETFE-EANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEK---YAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVS 4163
+C + + D++F++D S+S+ L Q++ F ++++ I ++G++ YSD L + ++K EI F+ L G T L+ E S+ ++G + +++TDG NS + EVE AE+L++ GI++ IG IKD +L K +T+ Y + + D + ++ Q+ C +P+ +A DI L+VD S + + + F + FI+ ++ + K + L +++ E D D+L+K+ S+++ G AL+ F+ ++ KN + VL+T+G+S + V+ + A L+ GV + G+ A+ +L+ ++ P +Q+F + L E + + K AI Q+ + + DI F+VD + + + IR+ + + LN+ + T+RI + +S+ EF D TK +I +I+ L+ G + A+N AR F A ++I F L ++ D + + +K + V +I VG+ + + +++AS S K+++ ++ + + ++C + ++ DV+ +DKS ++GPENFQ+ +F+ + LD+ D+ R+ I ++ E +V +++ +++ + + K+ + G G A+ A DY+FTK R E V ++ VV+TDG + S +SA A L G+ + +VG+ + KE+ ++V FT L + ++ C+ IP QK A D+ F++ S + T + F M F+ + + + R+ ++KF +R + D K+ E +++D+ G L + LFK TRK + ++I+I DG+ + A L+K + ++ +G+ + SP + +V N+ L+ I E+C + C+ D++F +D + ++ +Q D ++ V + I N ++ VV ++T+ + EF L++F +K I A+K++ G T G AL E S R V + +V+VTD S
Sbjct: 26 DCTQEAMADIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDG--NS---QDEVEPYAEELREKGIKIYAIG-----IKDADEKLLKEIATQPYDQHLFSVSDFEALQGI-MLNVTQEICTTVEREGEINSVPECNEFAAA--DIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVE--EPAQKLRMQGVITFVVKTGE-ANILQLRAIANSP----QQEFLFSGDSNQKLQELAENLRRKVCIAIDSQSQAFSLKFADIFFLVDSAA--SRQESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTP--AAGSRIAEGFKQFLLVATAGSSVDDVVQPSRQIKKEPVNLIAVGVGRPD-MDELKDIASPTLSYKLDSRTMPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKS----SDDVSAPAADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKLNALSG-VVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGVKNADTAELEDMSGSPKRTFYVQNYDALKLIKTKILKEICSF-----EACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQL---KGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSS 1122 HSP 2 Score: 172.555 bits (436), Expect = 1.492e-42 Identity = 253/1134 (22.31%), Postives = 500/1134 (44.09%), Query Frame = 3
Query: 939 TTTSNYIEI-STPDQNCNN-QQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD-IAQDAC-QIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD----DILKKVESLK-HVGGFTRIDKALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQT--C--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQ-VFKIDY--RAD--VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMIC----DAFKQRQDECTMKK-------LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGN---TAIGWAIGNATDYLFTKG----REDVPKVLVVLTDGAND-IGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE--FYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDN-FSNLQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALREC----KNELLNSTRKEVPKVIVLVTDG 4157
TT EI S P+ CN D++ ++D+S ++ + +E+ +F I+ L +++ ++G+ YSD LG +K ++ + + + G AL E F S+ R+ +L+TDG + +P A+KL+ G+ V+ G + I +S + + S L + + + + C I + S K DI +VD ++ Q + + F R + Q +G N R L F+ VEEEF D +I+ + +L+ G RI A+N A F+ + S K+ +++ T G S++ V +Q + +++ V++ +G+G + D +ELK +++ P L + + +P T C + D++F+VDKS + E + +R+ + T+ L++ R++I+DYSDE + L+ K +I +K+L + AL A+ VF + R+D V +I V+ TD + D + A L+ GV + VG+ + K + N K++ + IC F T++K D+ +D+S S+ ++F F+ + + D+ RIG+ F + +++ + ++K + + F+EG T +G A LF + +E V ++L+V+TDG ++ +G ++ A + + + I +VGV + EL D++ S + FY V+ + L+ K + S EAC D++F++ + + F+Q D + + + + R+A+++++ ++ E D + I + + G+ALT F+ R N + ++++ D + +++ A L++ + V+ +G+ G S+S+L ++ + +++N F +LQ + SEL +I N EC +L D++F +D + +I + +Q F+E +V ++ + G+V + A+ F L+Q+ K ++ A+ ++ G + +AL+ + VP+V+ L+T+G
Sbjct: 214 TTVEREGEINSVPE--CNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVEEP---AQKLRMQGVITFVVKTGEANILQLRAIANSPQQEFLFSGDSNQKLQELAENLRRKVCIAIDSQSQAFSLKFADIFFLVDSAASRQESQQIRN--FISRLVNQLNVGKNTNRIGLAQFSEN--VEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVDDV--VQPSRQIKKEPVNLIAVGVG-RPDMDELKDIAS---PTLSYKLDSRTMPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDG--KSSDDVSAPAADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKLNALSGVVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDL-FMEGGGTRTDLGLY---AMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFY-VQNYDALKLIKTKILKEICSFEACN--------DLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDS--SSKDDVVAPAMALREKNVNVYAVGM-GHASQSELLDISGSYETAYLENPFGSLQ----VLGSELVFMI--CNTECKRPELIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEG 1307 HSP 3 Score: 157.532 bits (397), Expect = 6.143e-38 Identity = 242/1108 (21.84%), Postives = 490/1108 (44.22%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLEL-LEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTT-----PAIK----PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTD--KLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAK---TISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF-NRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSARVLFKS----DTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPD-KALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTR--KEVPKVIVLVTDGVST 4166
D+ F++D+++S Q F + ++ L + +++G+ +S+ +E + + K EI + L+L +G I NA+ + F S+ EG K+ +++ T G+ ++P + ++KK + +I +G+G + D+L ++S Y ++ + L D+ + K C + D+ IVD S + + F + I ++G +K R ++V +++E + + +IL+ V+ L + G +AL A + K + S+ ++ V++T+G S + V A DL+ SGV + +G+ + LK ++++P F +F + +L K C+ P +K + C ++ DI F++D+S T + +D +++ +L L I RI ++ ++D T FRL+ +K + A+K L G G + L +FK + V +++++ TD P + AE L+ V I VG+ KN+ E+++ K + +F + C DV+ ID + ++ +FQ D ++ V+ L +R++R R+ + +++ + ++ ++ +QK+I I ++G T G A+ GR +V + LVV+TD ++ A++ K + + +VG+G S+ EL DI+ S E Y F L+ ++ I N C+ P+ D+IF++ + E F+ + FM+++ T V ++ R ++ + +R + + + Y E +I + T +AL S F + ++ + + +I +G + D + L + +F +G+ G E++L+ +A + +A +VD +S L+ I+ +LC++ K C + DL+ +D S S+ W T + +VR ++I + +V V F A F L+++ + ++ + +T+ ALR ++ E + +R V + ++L+TDG +T
Sbjct: 425 DIFFLVDSAASRQ-ESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVDDVVQP---SRQIKKEPVNLIAVGVGRPDM-DELKDIASPTLSYKLDSRTMPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSSDDVSA--PAADLRRSGVTVFAVGI---KSFDNLKEMASYPQDKFVFSVGNFTKLNALSGVVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVP-AEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFYVQNYDALKLIKTKILKEICSF----EACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSSKDDVVAPAMALREK--NVNVYAVGMGHA-SQSELLDISGSYETAYLENPFGSLQVLGSELVFMICNTECKRPE-------LIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFT-LNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSLADWPANLAASEVNMFAVGVAG-AREAELRRIAGNNQRAFYVDTYSQLETLCKPITQQLCNLTKPV---CEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGDAAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQDYFEPEHGSRMNARVSQNLLLITDGRAT 1501 HSP 4 Score: 133.65 bits (335), Expect = 1.019e-30 Identity = 161/717 (22.45%), Postives = 337/717 (47.00%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY-----RADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQRQD-----ECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQI--PQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGV-FIGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSN---LQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTR-----KEVPKVIVLVTDGVST 4166
DI+F+VD S + IR + + + +I+ RI ++ YSD TEF L+ K++I IK L G + L Q ++ + V +I V+ TD N +D + AE L+ G+KI +G+ K++ + +A++ + +++ + + + N+ IC ++ + EC D+++ +D S ++G F+ F+ +Q+LD+ + +IG+ ++ Y + + AD+ +++ I + G G A+ + F++ R++VP + V++TDG ++D E P A K ++ G+ V G+ + +L IA+S + ++ + L++ A+ + + C Q D+ F++ S+A++ + +Q+ +F+ L V K R+ + +F+ + F D E +SI ++ G+ IG A+ AR F S + + +++ G D + + + +KK+ + + +G+ G+ +LK +ASP + +D+ + Q+ A I S + ++C ++ D++F +D S ++GP+ +Q +F++ + +D+ + +V +V ++ + R + L+ F+ K ++ VK++ + G + GEAL+ K+ + R + V ++ V+VTDG S+
Sbjct: 34 DIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITD---GNSQDEVEPYAEELREKGIKIYAIGI-KDADEKLLKEIATQPYDQHLFSVSDFEALQGIMLNVTQEICTTVEREGEINSVPECNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVEEP---AQKLRMQGVITFVVKTGEA-NILQLRAIANSPQQEFLFSGDSNQKLQEL-AENLRRKVCIAIDSQSQAFSLKFADIFFLVDSAASR--QESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVD--DVVQPSRQIKKEPVNLIAVGV-GRPDMDELKDIASPTLSYKLDSRTMPVLPQQIKAVIDSPEGHKV---TEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSS 733 HSP 5 Score: 110.153 bits (274), Expect = 1.575e-23 Identity = 82/323 (25.39%), Postives = 156/323 (48.30%), Query Frame = 3
Query: 3255 KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFK--EDYKNFESSIEDMTTSTNGVFIGKALTSARVLFK--------SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNK-----ECST-KQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVST 4166
+A D++F++ SA+ F+Q+ +F+ SL + R+ +++++ + ED + I+ + T G G+ L + L + S +K + ++I DG D E + E L++ GIK++ IG+ E LK +A+ D+ LF V +F LQ ++ E+C ++ + EC+ D++ +DSS +IG +Y++ F+ ++D+D+N K+G+ ++ EF L ++T+K ++ + I + G + G AL + + RK VP + VL+TDGVS+
Sbjct: 30 EAMADIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGL---KFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDEVEPYAEE--LREKGIKIYAIGIK-DADEKLLKEIATQPYDQHLFSVSDFEALQGIMLNVTQEICTTVEREGEINSVPECNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSS 346 HSP 6 Score: 65.0846 bits (157), Expect = 6.915e-10 Identity = 38/138 (27.54%), Postives = 72/138 (52.17%), Query Frame = 3
Query: 3795 KGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-----NSTRKEVPKVIVLVTDGVSTGGLSPELE 4190
G +C+ + + D++F +D S+SIG +Q +F+ +V DI N ++G+V ++ R EF L+ + +K ++ +K + + G T+ GE L+ + +K VP++ V++TDG S + P E
Sbjct: 21 HGQKVDCTQEAMADIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDEVEPYAE 158 HSP 7 Score: 61.2326 bits (147), Expect = 1.130e-8 Identity = 45/143 (31.47%), Postives = 72/143 (50.35%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEF-LFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM---RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
DI ++D S+SI FQ ++ ++ F I PN R+ + Y DT +TEF L T L+F L K GG ++ G L L E R + +I ++++DG++ DE V+ A +++EKG+ IY + I
Sbjct: 34 DIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNT--GEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDE--VEPYAEELREKGIKIYAIGI 172 HSP 8 Score: 56.225 bits (134), Expect = 3.713e-7 Identity = 120/571 (21.02%), Postives = 228/571 (39.93%), Query Frame = 3
Query: 984 CNNQQL-DLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK--GR--EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDIL---KKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL-------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREE-LKILSTFPNPFLRQ-DFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRL----------DEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF-------KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGV-KIIFVGLSKNSPYNFYE 2567
C +L D+IF++D + ++ Q++ F V+ ++ K + G++IY+D + L NK+E+ + +SKL + G + AL+ + F + GR + + + L+T+G + A L + + +G+ L I + +TY +E + K I Q C + KP +C ++ D+ +++D S V +W + + E+ ++ R S+ F + A + + + Y+++ + + +L T AL Y + ++ R+ ++L+T+G + ++ + + L+ V+I +G+G +R LKI + FL + K+ K ++ C PQ CS + I F V + T + + L+ + L++ + ET FR+ D F K D V K L +L A A F K+ K+V+LFTD + +E L+ GV ++ V L+ N Y E
Sbjct: 1188 CKRPELIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSLADWPA-NLAASEVNMFAVGVAGAREAELRRIAGNNQRAFYVDTYSQLETLCK-------PITQQLCNLTKP--VCEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGD-AAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQD--YFEPEHGSRMNARVSQNLLLITDGRATDK-EDLGVLASLRAKNVEIFAVGIGKNINRHALLKIAGSKERVFLESFESLPLKTTTAKVLQAICSPDEIRDPQGCSIDIGIGFDVSRRTSSQPLLGPHSEPLVAAAIHHLSMMGALCCLPADKI--ETKIGFRVVSGRDGRVLDDFNFEKYDENVVRKVL-------ELRPATPTAFNAFLLNSFMQKLSSSQAGVKVVILFTDGFDDTLEHLKESSESLRKSGVDALLIVALNGNVNYQQLE 1735 HSP 9 Score: 51.6026 bits (122), Expect = 9.961e-6 Identity = 42/146 (28.77%), Postives = 69/146 (47.26%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVR-LHRKVGGDSSVLGRALNSTLYDIVP-----EMRPTSEIDRILLILSDG-SNTDELSVKDLATKIQEKGVTIYTVAI 566
DI +LD S SIT +F I +FL F I P+ R+ + + D F + S + AV+ L + GG + LG LY ++P E ++ +L++++DG S VK A +++++ VTIY V +
Sbjct: 821 DIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLG------LYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV 960
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: si:ch211-62a1.3 (si:ch211-62a1.3 [Source:ZFIN;Acc:ZDB-GENE-061207-41]) HSP 1 Score: 210.69 bits (535), Expect = 3.485e-54 Identity = 270/1135 (23.79%), Postives = 519/1135 (45.73%), Query Frame = 3
Query: 981 NCNNQQL-DLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALR-----RSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKD---QLNKMSSTETY----YPIEDMSKMALFDTTDIAQDAC----------QIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKS-KNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFX----XXXXXXXXXXXXXCKTTPAIKPQTCSSN---GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNG-GDLNTALNRARQVFKIDYRADVAKIVVLFTDKL-----PTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKL--GGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQ------IP-------QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF-NRGNYIDSGFKEDYKN-FESSIEDMTTSTNGVFIGKALTSARVLFKS--DTRKNATKTVIIII-DGEITDRNETFE-EANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEK---YAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVS 4163
+C + + D++F++D S+S+ L Q++ F ++++ I ++G++ YSD L + ++K EI F+ L G T L+ E S+ ++G + +++TDG NS + EVE AE+L++ GI++ IG IKD +L K +T+ Y + + D + ++ Q+ C +P+ +A DI L+VD S + + + F + FI+ ++ + K + L +++ E D D+L+K+ S+++ G AL+ F+ ++ KN + VL+T+G+S + V+ + A L+ GV + G+ A+ +L+ ++ P +Q+F + L E + + K AI Q+ + + DI F+VD + + + IR+ + + LN+ + T+RI + +S+ EF D TK +I +I+ L+ G + A+N AR F A ++I F L ++ D + + +K + V +I VG+ + + +++AS S K+++ ++ + + ++C + ++ DV+ +DKS ++GPENFQ+ +F+ + LD+ D+ R+ I ++ E +V +++ +++ + + K+ + G G A+ A DY+FTK R E V ++ VV+TDG + S +SA A L G+ + +VG+ + KE+ ++V FT L + ++ C+ IP QK A D+ F++ S + T + F M F+ + + + R+ ++KF +R + D K+ E +++D+ G L + LFK TRK + ++I+I DG+ + A L+K + ++ +G+ + SP + +V N+ L+ I E+C + C+ D++F +D + ++ +Q D ++ V + I N ++ VV ++T+ + EF L++F +K I A+K++ G T G AL E S R V + +V+VTD S
Sbjct: 26 DCTQEAMADIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDG--NS---QDEVEPYAEELREKGIKIYAIG-----IKDADEKLLKEIATQPYDQHLFSVSDFEALQGI-MLNVTQEICTTVEREGEINSVPECNEFAAA--DIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVE--EPAQKLRMQGVITFVVKTGE-ANILQLRAIANSP----QQEFLFSGDSNQKLQELAENLRRKVCIAIDSQSQAFSLKFADIFFLVDSAA--SRQESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTP--AAGSRIAEGFKQFLLVATAGSSVDDVVQPSRQIKKEPVNLIAVGVGRPD-MDELKDIASPTLSYKLDSRTMPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKS----SDDVSAPAADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKLNALSG-VVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGVKNADTAELEDMSGSPKRTFYVQNYDALKLIKTKILKEICSF-----EACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQL---KGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSS 1122 HSP 2 Score: 172.555 bits (436), Expect = 1.492e-42 Identity = 253/1134 (22.31%), Postives = 500/1134 (44.09%), Query Frame = 3
Query: 939 TTTSNYIEI-STPDQNCNN-QQLDLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD-IAQDAC-QIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD----DILKKVESLK-HVGGFTRIDKALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQT--C--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQ-VFKIDY--RAD--VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMIC----DAFKQRQDECTMKK-------LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGN---TAIGWAIGNATDYLFTKG----REDVPKVLVVLTDGAND-IGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE--FYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD--TRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDN-FSNLQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALREC----KNELLNSTRKEVPKVIVLVTDG 4157
TT EI S P+ CN D++ ++D+S ++ + +E+ +F I+ L +++ ++G+ YSD LG +K ++ + + + G AL E F S+ R+ +L+TDG + +P A+KL+ G+ V+ G + I +S + + S L + + + + C I + S K DI +VD ++ Q + + F R + Q +G N R L F+ VEEEF D +I+ + +L+ G RI A+N A F+ + S K+ +++ T G S++ V +Q + +++ V++ +G+G + D +ELK +++ P L + + +P T C + D++F+VDKS + E + +R+ + T+ L++ R++I+DYSDE + L+ K +I +K+L + AL A+ VF + R+D V +I V+ TD + D + A L+ GV + VG+ + K + N K++ + IC F T++K D+ +D+S S+ ++F F+ + + D+ RIG+ F + +++ + ++K + + F+EG T +G A LF + +E V ++L+V+TDG ++ +G ++ A + + + I +VGV + EL D++ S + FY V+ + L+ K + S EAC D++F++ + + F+Q D + + + + R+A+++++ ++ E D + I + + G+ALT F+ R N + ++++ D + +++ A L++ + V+ +G+ G S+S+L ++ + +++N F +LQ + SEL +I N EC +L D++F +D + +I + +Q F+E +V ++ + G+V + A+ F L+Q+ K ++ A+ ++ G + +AL+ + VP+V+ L+T+G
Sbjct: 214 TTVEREGEINSVPE--CNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVEEP---AQKLRMQGVITFVVKTGEANILQLRAIANSPQQEFLFSGDSNQKLQELAENLRRKVCIAIDSQSQAFSLKFADIFFLVDSAASRQESQQIRN--FISRLVNQLNVGKNTNRIGLAQFSEN--VEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVDDV--VQPSRQIKKEPVNLIAVGVG-RPDMDELKDIAS---PTLSYKLDSRTMPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDG--KSSDDVSAPAADLRRSGVTVFAVGIKSFDNLKEMASYPQDKFVFSVGNFTKLNALSGVVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDL-FMEGGGTRTDLGLY---AMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFY-VQNYDALKLIKTKILKEICSFEACN--------DLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDS--SSKDDVVAPAMALREKNVNVYAVGM-GHASQSELLDISGSYETAYLENPFGSLQ----VLGSELVFMI--CNTECKRPELIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEG 1307 HSP 3 Score: 157.532 bits (397), Expect = 6.143e-38 Identity = 242/1108 (21.84%), Postives = 490/1108 (44.22%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLEL-LEGATFISNALRRSGEEFI-----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTT-----PAIK----PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI---DYRADVAKIVVLFTD--KLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL--FTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAK---TISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF-NRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSARVLFKS----DTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPD-KALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTR--KEVPKVIVLVTDGVST 4166
D+ F++D+++S Q F + ++ L + +++G+ +S+ +E + + K EI + L+L +G I NA+ + F S+ EG K+ +++ T G+ ++P + ++KK + +I +G+G + D+L ++S Y ++ + L D+ + K C + D+ IVD S + + F + I ++G +K R ++V +++E + + +IL+ V+ L + G +AL A + K + S+ ++ V++T+G S + V A DL+ SGV + +G+ + LK ++++P F +F + +L K C+ P +K + C ++ DI F++D+S T + +D +++ +L L I RI ++ ++D T FRL+ +K + A+K L G G + L +FK + V +++++ TD P + AE L+ V I VG+ KN+ E+++ K + +F + C DV+ ID + ++ +FQ D ++ V+ L +R++R R+ + +++ + ++ ++ +QK+I I ++G T G A+ GR +V + LVV+TD ++ A++ K + + +VG+G S+ EL DI+ S E Y F L+ ++ I N C+ P+ D+IF++ + E F+ + FM+++ T V ++ R ++ + +R + + + Y E +I + T +AL S F + ++ + + +I +G + D + L + +F +G+ G E++L+ +A + +A +VD +S L+ I+ +LC++ K C + DL+ +D S S+ W T + +VR ++I + +V V F A F L+++ + ++ + +T+ ALR ++ E + +R V + ++L+TDG +T
Sbjct: 425 DIFFLVDSAASRQ-ESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVDDVVQP---SRQIKKEPVNLIAVGVGRPDM-DELKDIASPTLSYKLDSRTMPVLPQQIKAVIDSPEGHKVTEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSSDDVSA--PAADLRRSGVTVFAVGI---KSFDNLKEMASYPQDKFVFSVGNFTKLNALSGVVTKRICRDISSVFIPLLKNITIQKGCKFTAEADIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLGLYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVP-AEELRKQNVTIYAVGV-KNADTAELEDMSGSPKRTFYVQNYDALKLIKTKILKEICSF----EACNDLLADVVFLIDGADAVDAADFQQAKDLIDFAVEKLPIRENRVRLAVVQYSTNTKVEFALNEFYDKDRIQKQIVAIKQLKGKTYTGRALTEVWQAFEESRGGRPNVLRFLVVVTDSSSKDDVVAPAMALREK--NVNVYAVGMGHA-SQSELLDISGSYETAYLENPFGSLQVLGSELVFMICNTECKRPE-------LIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFT-LNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSLADWPANLAASEVNMFAVGVAG-AREAELRRIAGNNQRAFYVDTYSQLETLCKPITQQLCNLTKPV---CEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGDAAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQDYFEPEHGSRMNARVSQNLLLITDGRAT 1501 HSP 4 Score: 133.65 bits (335), Expect = 1.019e-30 Identity = 161/717 (22.45%), Postives = 337/717 (47.00%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY-----RADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQRQD-----ECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQI--PQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGV-FIGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSN---LQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTR-----KEVPKVIVLVTDGVST 4166
DI+F+VD S + IR + + + +I+ RI ++ YSD TEF L+ K++I IK L G + L Q ++ + V +I V+ TD N +D + AE L+ G+KI +G+ K++ + +A++ + +++ + + + N+ IC ++ + EC D+++ +D S ++G F+ F+ +Q+LD+ + +IG+ ++ Y + + AD+ +++ I + G G A+ + F++ R++VP + V++TDG ++D E P A K ++ G+ V G+ + +L IA+S + ++ + L++ A+ + + C Q D+ F++ S+A++ + +Q+ +F+ L V K R+ + +F+ + F D E +SI ++ G+ IG A+ AR F S + + +++ G D + + + +KK+ + + +G+ G+ +LK +ASP + +D+ + Q+ A I S + ++C ++ D++F +D S ++GP+ +Q +F++ + +D+ + +V +V ++ + R + L+ F+ K ++ VK++ + G + GEAL+ K+ + R + V ++ V+VTDG S+
Sbjct: 34 DIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITD---GNSQDEVEPYAEELREKGIKIYAIGI-KDADEKLLKEIATQPYDQHLFSVSDFEALQGIMLNVTQEICTTVEREGEINSVPECNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSSDAVEEP---AQKLRMQGVITFVVKTGEA-NILQLRAIANSPQQEFLFSGDSNQKLQEL-AENLRRKVCIAIDSQSQAFSLKFADIFFLVDSAASR--QESQQIRNFISRLVNQLNVGKNTNRIGLAQFSENVEEEFLFDNDKTKNEIVASIRNLQLKPKGLRRIGNAINHARTRFFTPAAGSRIAEGFKQFLLVATAGSSVD--DVVQPSRQIKKEPVNLIAVGV-GRPDMDELKDIASPTLSYKLDSRTMPVLPQQIKAVIDSPEGHKV---TEDCRLAEVADVVFIVDKSGNMGPENFQLVRNFLQNTISGLDVGTDKVRVAIVDYSDEPRADVYLNTFSNKSEILQHVKKLSYGRGKANTGEALKYAKDYVFTKERGSRSDEYVQQIAVVVTDGKSS 733 HSP 5 Score: 110.153 bits (274), Expect = 1.575e-23 Identity = 82/323 (25.39%), Postives = 156/323 (48.30%), Query Frame = 3
Query: 3255 KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFK--EDYKNFESSIEDMTTSTNGVFIGKALTSARVLFK--------SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNK-----ECST-KQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVST 4166
+A D++F++ SA+ F+Q+ +F+ SL + R+ +++++ + ED + I+ + T G G+ L + L + S +K + ++I DG D E + E L++ GIK++ IG+ E LK +A+ D+ LF V +F LQ ++ E+C ++ + EC+ D++ +DSS +IG +Y++ F+ ++D+D+N K+G+ ++ EF L ++T+K ++ + I + G + G AL + + RK VP + VL+TDGVS+
Sbjct: 30 EAMADIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGL---KFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDEVEPYAEE--LREKGIKIYAIGIK-DADEKLLKEIATQPYDQHLFSVSDFEALQGIMLNVTQEICTTVEREGEINSVPECNEFAAADIVLLVDSSDNIGENYFREIQSFLRVFIQDLDVNVGKVKIGLAQYSDRPYPEFLLGEYTDKADLLRKLGSIRYRRGRANTGAALDFIREGFFSQARKNVPHIAVLITDGVSS 346 HSP 6 Score: 65.0846 bits (157), Expect = 6.915e-10 Identity = 38/138 (27.54%), Postives = 72/138 (52.17%), Query Frame = 3
Query: 3795 KGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-----NSTRKEVPKVIVLVTDGVSTGGLSPELE 4190
G +C+ + + D++F +D S+SIG +Q +F+ +V DI N ++G+V ++ R EF L+ + +K ++ +K + + G T+ GE L+ + +K VP++ V++TDG S + P E
Sbjct: 21 HGQKVDCTQEAMADIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNTGEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDEVEPYAE 158 HSP 7 Score: 61.2326 bits (147), Expect = 1.130e-8 Identity = 45/143 (31.47%), Postives = 72/143 (50.35%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEF-LFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM---RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
DI ++D S+SI FQ ++ ++ F I PN R+ + Y DT +TEF L T L+F L K GG ++ G L L E R + +I ++++DG++ DE V+ A +++EKG+ IY + I
Sbjct: 34 DIVFLVDGSASIGLTNFQQIREFLSSLVTHFDIAPNKVRIGLVQYSDTPRTEFSLNTYEDKQEILDFIKVLPYKTGGTNT--GEGLKFLLQHHFVEAAGSRAKKGVPQIAVVITDGNSQDE--VEPYAEELREKGIKIYAIGI 172 HSP 8 Score: 56.225 bits (134), Expect = 3.713e-7 Identity = 120/571 (21.02%), Postives = 228/571 (39.93%), Query Frame = 3
Query: 984 CNNQQL-DLIFVLDASSSLALYWDQELA-FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK--GR--EGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLG------LSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDIL---KKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL-------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREE-LKILSTFPNPFLRQ-DFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRL----------DEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF-------KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGV-KIIFVGLSKNSPYNFYE 2567
C +L D+IF++D + ++ Q++ F V+ ++ K + G++IY+D + L NK+E+ + +SKL + G + AL+ + F + GR + + + L+T+G + A L + + +G+ L I + +TY +E + K I Q C + KP +C ++ D+ +++D S V +W + + E+ ++ R S+ F + A + + + Y+++ + + +L T AL Y + ++ R+ ++L+T+G + ++ + + L+ V+I +G+G +R LKI + FL + K+ K ++ C PQ CS + I F V + T + + L+ + L++ + ET FR+ D F K D V K L +L A A F K+ K+V+LFTD + +E L+ GV ++ V L+ N Y E
Sbjct: 1188 CKRPELIDIIFLVDGAGAINKETFQDIKIFMETVVTKTEVGEKRVRFGLVIYADRAQSVFTLNQYYNKSEVLEAISKLRTIGGRRNTAQALKSTLPYFSAAYGGRRAQHVPQVLFLITEGPVADSFSLADWPA-NLAASEVNMFAVGVAGAREAELRRIAGNNQRAFYVDTYSQLETLCK-------PITQQLCNLTKP--VCEKEVADLVILIDGSESVSALDWGTLKSSLINLVRKLEIAQDRWRVSVAQFGD-AAPDRFYLNKYNNLAGVEQGIRALSQRRQSTNTWTALRFLQD--YFEPEHGSRMNARVSQNLLLITDGRATDK-EDLGVLASLRAKNVEIFAVGIGKNINRHALLKIAGSKERVFLESFESLPLKTTTAKVLQAICSPDEIRDPQGCSIDIGIGFDVSRRTSSQPLLGPHSEPLVAAAIHHLSMMGALCCLPADKI--ETKIGFRVVSGRDGRVLDDFNFEKYDENVVRKVL-------ELRPATPTAFNAFLLNSFMQKLSSSQAGVKVVILFTDGFDDTLEHLKESSESLRKSGVDALLIVALNGNVNYQQLE 1735 HSP 9 Score: 51.6026 bits (122), Expect = 9.961e-6 Identity = 42/146 (28.77%), Postives = 69/146 (47.26%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVR-LHRKVGGDSSVLGRALNSTLYDIVP-----EMRPTSEIDRILLILSDG-SNTDELSVKDLATKIQEKGVTIYTVAI 566
DI +LD S SIT +F I +FL F I P+ R+ + + D F + S + AV+ L + GG + LG LY ++P E ++ +L++++DG S VK A +++++ VTIY V +
Sbjct: 821 DIYFLLDESGSITYDDFDDMKAFILEFLHMFQIGPDQVRIGVVKFADRATIVFRLNTYDSKSAVEKAVKDLFMEGGGTRTDLG------LYAMIPLFKQAEQTRKEKVRELLIVITDGKSEPVGTPVKVPAEELRKQNVTIYAVGV 960
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: col6a4a (collagen, type VI, alpha 4a [Source:ZFIN;Acc:ZDB-GENE-041111-303]) HSP 1 Score: 202.216 bits (513), Expect = 8.094e-52 Identity = 268/1107 (24.21%), Postives = 482/1107 (43.54%), Query Frame = 3
Query: 1008 IFVLDASSSLALYWDQELA----FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLE-GATFISNAL-----RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVI----KDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQ--DFXXXXXXXXXXXXXXC-----KTTPAIK----PQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYR----ADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF-TKG-REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALT-SARVLFKSDTRK---NATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKE-----VPKVIVLVTDGVS 4163
+FVL SSS Q++A F + L + K +++ ++ + + + L + + E + K +L G + + A+ R E S+ +G K+ +++V G ++ ++ LK G++++ + L+ I KD +N + D ++ D C+ SA++ DI IVD S + + F R I+ ++G + R +V + NE E + +D DIL+ ++ L + GG KAL A + K S+R + ++T G S + V + A L+ SGV + +G+ K + ++I S P ++ F + + + K C +T I Q C + DI F++D S + ++ +L L NI R+ ++ + T EF + + + I A+ + G G + AL R F I+ R V +I+++ TD T++ + A LK GV I VG+ + + E +S +EN + + K++I IC +D C D+I +D S S+G E F FVN V L + KD ++G+ F + ++S ++ M + +++ E N IG A+ + TKG R++ PK L+++T + + A++ K G+ I S+G+G+ S +L +I+ S + ++ D + + + C + + A D++F++ S + E F +M FM + T V + R + ++ + K+ Y E ++I ++ T + AL S R K++ + + + +I DGE TD + A L GI VF IG+ G+ E +L ++AS K +F VDN+ L+ + IS LC+ + ECS DL+ +D S SI W T ++F+ R V + + + ++GV F+T +KEF L++ E A+ A+K I T+IG AL + +E S + + ++L+TDG S
Sbjct: 229 VFVLVDSSS------QQIANIRKFLIQLATQLDVGAKVNRMALIQFGGEVNVEFLLNAYNTRREAIALIQKFQLRSSGRSNLGQAMDYVRTRLLNSESGSRIAQGFKQYLLVVGKGETDTAVLRA---TRALKAEGVKIVEVDTNLTNIVQDVKDSIN------------------VPDVISVSGD-CK--------SAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEF-YLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGESPDDVTS--EASKLRRSGVRVYALGIQHKNVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPYF-IEARKTRDTRVREILIVITDGESTDEVKQPAAA--LKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDICS-----EDACKQMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITGAESKDDVNAPAKALRDK--GVTIYSIGIGEAKS-LQLRNISGSPKQVYMERNLDALDLLDENLLLKICNSADGESKTQVA-DVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSPAGKLHALGINVFGIGV-GEAKEDELMTIASDPKNVFKVDNYEALKALHQNISKVLCN---NSKPECSKVAADLVILIDGSESIEDAPWNTMINFMMRFVDSLRVREDLFRIGVAQFSTVYKKEFYLNEHMEPAAIKKAIKDIKQMKDGTNIGNALYQV-DEFFQSAAGSRINSGISQNLLLITDGKS 1279 HSP 2 Score: 160.614 bits (405), Expect = 5.316e-39 Identity = 244/1130 (21.59%), Postives = 500/1130 (44.25%), Query Frame = 3
Query: 945 TSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQEL----AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK-GREGAKKTV----ILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFD-TTDIAQDAC-QIPKPKRICSAKL-DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHV----GGFTRIDKALNLASRSFYLKSKNSKRLV-----VLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQ-VFKIDYRADVAKIV--VLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNS-----------PYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMIC--DAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASS-GEFYNVKTFTDLEKFNAKTI----SNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVD-NFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNEL--LNSTRKE--VPKVIVLVTDGVST 4166
+S Y+ S +C D++F++D SSS+ D + F + I+ L+I +V V+ +S+ +PLG +K E+ + V +L G+T AL + + ++ GR A+ V +++TDG D++ MK A++L++ G+ + IG+G + + + + + I ++ AL + +++ C + +R + K D+ ++VD SSQ + N F + Q ++G R +L+ F E V E + Y+ + + ++ G + + +A++ R+ L S++ R+ LL G ++A L+ GV I + +++K P+ SV CK+ DI+F+VD S ++ ++ +R + ++ L++ + RI ++ Y +E EF L K DI I+ L G + + AL AR+ VF + + A+ V + F + D SEA L+ GV++ +G+ + P + N+ S +E + ++ + + F +Q ++ D+ ID S S+ P +FQ F+ ++ + ++ + R+G+ F S L + + A ++ ++ I I G T G A+ Y F + R+ V ++L+V+TDG + +A+K++ G+ I +VGV +Q ++ +L ++ASS + + V+ + L+ + + S +AC+ + D+IF++ S + +E F ++ F+ + + K++ +V +++F R + + + + + + + + TN IGKAL F R+NA K +I+I E +++ A L+ G+ +++IG+ G+ L++++ K ++++ N L + + ++C+ G E T+ D++F +D S+SI + + F+ +V + ++ N+ + + ++ +F L + K + +A+ + G T AL+ R E VP+++ ++TDG +T
Sbjct: 14 SSCYLSSSAQKTDCRANLADIVFLVDGSSSIG---DADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNFIKDRYFTQAGRIQARANVPRIAVVITDG-DSTDDMK--TPAQELRREGVLIFTIGVGEVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTVCISMEDKQRALAPKYSDVFVLVDSSSQ----QIANIRKFLIQLATQLDVGAKVNRMALIQFGGEVNV-EFLLNAYNTRREAIALIQKFQLRSSGRSNLGQAMDYV-RTRLLNSESGSRIAQGFKQYLLVVGKGETDTAVLRATRALKAEGVKIVEVDTNLTNIVQDVKDSINVPD--------------VISVSGDCKSAQV---------ADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGESPDDVTSEASKLRRSGVRVYALGIQHKNVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFMRPY-FIEARKTRDTRVREILIVITDGESTDEVKQPAAALKSQ--GVSIYAVGV-KQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDICSEDACK--------QMMADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPE-IGKALNELPKFFGPTKGGRQNAPKFLILITGAE--SKDDVNAPAKALRDKGVTIYSIGI-GEAKSLQLRNISGSPKQVYMERNLDALDLLDENLLLKICNSADG---ESKTQVADVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYTASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEAT 1089 HSP 3 Score: 152.91 bits (385), Expect = 1.160e-36 Identity = 273/1183 (23.08%), Postives = 505/1183 (42.69%), Query Frame = 3
Query: 963 ISTPD-----QNCNNQQL-DLIFVLDASSSLA-----LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGE-----EFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD--QLNKMSSTETYYPIEDMSKMALFD--------TTDIAQDACQIPK--PKRIC--SAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS-----KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI--DYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNFENADMQKEIN------KIPFIEGNTAIGWAIGNATDYLFTK---GRED--VPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN-------FESSIEDMTTSTNGVFIGKALTSARVLFKS--DTRKNA--TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALD------SSSSI--GPDYWQTELDFVER------------------------IVRD--MDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKR----------------IIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVST 4166
I+ PD +C + Q+ D++F++DAS +++ L D F + +IK L + ++G+++Y + + L + NK +I +++ L G S AL+ + E E S+ G + ++T+G SP EA KL++ G+RV +G+ + Q+ + Y ++ +K+ + + + + +I + K+ C + + DI ++D S + ++ + F + F +G + R +V F + +E T D I V ++ +GG T +AL R ++++++ + + +++++T+G S + V+ Q A L+ GV I +G+ + L++ S+ F +++ K L ++ V C + A K DIIF+VD S E + I+ + T+ L I + + ++ ++ + TEF+L+ + K + A+ Q ++ ALN + F R + K ++L T ++ D + A+ L+ GV I +G+ + S K N++ +D E + IC++ E + DV+ +D STS+ E+F F+N +V N ++ ++ R F + VY N P + N + ++E+N K P G+T A+ + + F+K GR + VP+++ V+TDG A D + P A K GI + +GVG+ E EL IAS + + V + L+ + + IS C KP K A DL+ ++ S + + M +FM + VD R + + + KE Y N + +I+D+ +G IG AL F+S +R N+ ++ +++I DG+ D + + L+ I +F IG+ G+ S ++ +A S D+ +D+F L+ + C + EC+ +D+ D SS S+ G + QT L + R + RD M + NF T+N + K+ Q + A S + R IIF DG D + L +L NS V ++++ DGV +
Sbjct: 376 INVPDVISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRD----FLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEG--ESPD-DVTSEASKLRRSGVRVYALGIQHKNVTQLMQIASYPPRKYVYNVDSFAKLTIMEQFLRKTLCNNVMRRTVAKINQFNIKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTETGRALTFM-RPYFIEARKTRDTRVREILIVITDGESTDEVK--QPAAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDIC-SEDACKQMM----ADIIFLVDGSWSIGAEGFSKIKKFVNNTVSRLKIGKDSVQVGLMQFGTNPRTEFQLNSYYDKVQMMRAVSQTVHAETNPEIGKALNELPKFFGPTKGGRQNAPKFLILITG--AESKDDVNAPAKALRDKGVTIYSIGIGEAKSLQLRNISGSPKQVYMERNLDALDLLDENLLLKICNS---ADGESKTQVADVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVR-----FCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAP--TGDTYTASALQYSIRF-FSKAEGGRAEDGVPQLMFVITDGEATDHWDLPS-PAGKLHALGINVFGIGVGEA-KEDELMTIASDPKNVFKVDNYEALKALH-QNISKVLCN-NSKPECSKVAADLVILIDGSESIEDAPWNTMINFM-----MRFVDSLRVREDLFRIGVAQFSTVYKKEFYLNEHMEPAAIKKAIKDIKQMKDGTNIGNALYQVDEFFQSAAGSRINSGISQNLLLITDGKSND--NIYNATDRLRARNINMFVIGI-GQISMKQIEYIAGSQDRVFVLDSFDALKLNTTILEVINCISVALLLTECT---VDIGVGFDVSRISASSQSLFTGQNKLQTYLPEIIRYISTLNNLCCLKEPTLQTKTGFQVVARDGTMLYDTNFE-----TYNEEVIKKLLAVQIAQPLAFNSQLLRSFQLKHAASTTGVKIVIIFSDGLDDSADVLMRASQDLRNSG---VDALLIVALDGVQS 1507 HSP 4 Score: 102.834 bits (255), Expect = 2.201e-21 Identity = 111/530 (20.94%), Postives = 244/530 (46.04%), Query Frame = 3
Query: 2670 RQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKF--NAKTISNEAC-QIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSL-TKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESS----IEDMTTSTNGVF-IGKALTSARVLF---KSDTR-KNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLK-SVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNEL----LNSTR-KEVPKVIVLVTDGVSTGGLSPE 4184
++ +C D++ +D S+S+G +F F++ +Q L++ D R+ + F++ + ++P+ + ++ ++++++ + G+T G A+ D FT + R +VP++ VV+TDG D + + A + + G+ I ++GVG +++ EL IA+ V +F + E A + N C + K +FV+ + +S++ + F+ L T+L V K N R+A+++F G ++ F + N I+ ++G +G+A+ R +S +R K ++++ TD LK +G+K+ + D+K S+ PD + + +C + Q+ D++F +D+S +I + DF+ R+++ +D+ ++ ++G+V + + + EF L F K ++ ++ + + G + +AL+ + ++ L S R + V ++ ++T+G S ++ E
Sbjct: 23 QKTDCRANLADIVFLVDGSSSIGDADFLRMKRFLHAFIQGLEISADSIRVAVVQFSNNPHEDIPLGDYADKNELLEKVDRLVYHRGSTETGKALNFIKDRYFTQAGRIQARANVPRIAVVITDG--DSTDDMKTPAQELRREGVLIFTIGVG-EVNMAELQSIANKPHQRFVISFVNYEALVNAASNMMNTVCISMEDKQRALAPKYSDVFVL---VDSSSQQIANIRKFLIQLATQLDVGAKVN-RMALIQF--GGEVNVEFLLNAYNTRREAIALIQKFQLRSSGRSNLGQAMDYVRTRLLNSESGSRIAQGFKQYLLVVGKGETD-TAVLRATRALKAEGVKIVEVDTNLTNIVQDVKDSINVPD-------------------------VISVSGDCKSAQVADIVFIVDASDAISDSNFNLVRDFLHRLIKGLDVGSDSMRIGMVLYGNEPKAEFYLKTFDNKPDILQYIQILPYKGGKANTSKALKFAREKVFSKELGSRRARGVLQIAFVITEGESPDDVTSE 517 HSP 5 Score: 59.3066 bits (142), Expect = 3.734e-8 Identity = 54/191 (28.27%), Postives = 92/191 (48.17%), Query Frame = 3
Query: 120 LDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPT--SEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
+ Q C+ + DI ++D S SI +FQ I + L F I P RV + + T EF T++ ++ AV ++GG + GRAL + + E R T + + IL++++DG +TDE VK A ++ +GV+IY V + LL SS + + +++ + +V+ IC
Sbjct: 590 IKQGCVQTEEADIYFLIDHSGSIDPPDFQDMKKFILEVLLHFNIGPKQVRVGVVKFESTPTLEFTVTQYGDRASIEGAVSNIVQIGGGTET-GRAL-TFMRPYFIEARKTRDTRVREILIVITDGESTDE--VKQPAAALKSQGVSIYAVGVKQANKTQLLEMASSPSKMFFVENYDSLKPLKDEIVTDIC 776 HSP 6 Score: 52.7582 bits (125), Expect = 3.729e-6 Identity = 38/180 (21.11%), Postives = 81/180 (45.00%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTL--YDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
D+ ++D S+SI +F I +N ++ + N R + Y +T + +F KH + +N A+ + GD+ AL ++ + R + +++ +++DG TD + A K+ G+ ++ + +G L+ S KNV ++ ++ +N+ V+C
Sbjct: 974 DVVFLVDGSTSIAEEDFDKMKIFMNLVVNNTEVGENYVRFCTIVYSNTPEVKFNLKKHYTKREVNNAISELKAPTGDTYT-ASALQYSIRFFSKAEGGRAEDGVPQLMFVITDGEATDHWDLPSPAGKLHALGINVFGIGVGEAKEDELMTIASDPKNVFKVDNYEALKALHQNISKVLC 1152
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Match: si:ch1073-291c23.1 (vitrin [Source:HGNC Symbol;Acc:HGNC:12697]) HSP 1 Score: 107.457 bits (267), Expect = 4.031e-23 Identity = 85/349 (24.36%), Postives = 162/349 (46.42%), Query Frame = 3
Query: 3189 EKFNAKTISNEACQIPQKPTI-------CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP---DKA---------LFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
E N + + E +P+ P I CK D+ F+M S + RFK DF+ ++++ V + I+++ + ++ + K + +I+ + +GKAL+ + SD R A ++++DG TD+ E E + ++ GI +F + + G +++ +V P DKA L V ++ L++ + +CD + + D+ F +D SSS+G ++T L FV + R+ +I+ T+VG V + + R EF Q K +++A+KRI + G T G A+ +L + ++ K+++++TDG S
Sbjct: 312 ENSNPRFSTREQDSLPRAPEIMSQGDPNCKV--DIAFLMDGSWSIGKRRFKIQKDFLAEVSQVINVGVAGPMMGIIQYGDDPVTEFSLRQYSNSKELKPAIDKIMQKGGLSNVGKALSYINKHYFSDANGNRGGAPNVAVVLVDGWPTDKVE--EASRQARESGINIFFVTIEG-PDDNEKHNVVEPNFVDKAVCRTNGFFSLPVASWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQHNTKADLLNAIKRINYWSGGTSTGAAITYAAEQLFSKSKPNKRKIMIVITDGRS 655 HSP 2 Score: 78.9518 bits (193), Expect = 2.619e-14 Identity = 79/341 (23.17%), Postives = 148/341 (43.40%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFV---GLSKNSPYNFYE-NLAXXXXXXX-------XXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGAN-DIGESPQISAVKAKLGGIKIISVGV 3107
DI F++D S + + + Q +N+ + II Y D+ TEF L + +++ AI ++ G ++ AL+ + + D R + V+ D PT++ + S + G+ I FV G N +N E N K + + + K + + +CD + + + D+ ID S+S+G NF+ L FV + + ++ TR+G + E L + AD+ I +I + G T+ G AI A + LF+K + + K+++V+TDG + D +P ++ ++ G+ S+G+
Sbjct: 343 DIAFLMDGSWSIGKRRFKIQKDFLAEVSQVINVGVAGPMMGIIQYGDDPVTEFSLRQYSNSKELKPAIDKIMQKGGLSNVGKALSYINKHYFSDANGNRGGAPNVAVVLVDGWPTDKVEEASRQA--RESGINIFFVTIEGPDDNEKHNVVEPNFVDKAVCRTNGFFSLPVASWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQHNTKADLLNAIKRINYWSGGTSTGAAITYAAEQLFSKSKPNKRKIMIVITDGRSYDDVRAPALAVHRS---GVIAYSIGI 678 HSP 3 Score: 70.8626 bits (172), Expect = 8.391e-12 Identity = 81/358 (22.63%), Postives = 153/358 (42.74%), Query Frame = 3
Query: 963 ISTPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIA-----QDACQIPKPKRICSAKL-----------DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--THIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGL 1967
+S D NC ++D+ F++D S S+ + + F V + + + + +G++ Y D L N E++ + K+ G + + AL + + S R GA +++ DG PT K E + + ++ GI + + + D K + E + + + + F + +A + A Q P KR+C DIA ++D SS V + L F +FE+ TR V + E +E F + D+L ++ + + G T A+ A+ + KSK N +++++++T+G S + V+ A + SGV +IG+
Sbjct: 333 MSQGDPNC---KVDIAFLMDGSWSIGKRRFKIQKDFLAEVSQVINVGVAGPMMGIIQYGDDPVTEFSLRQYSNSKELKPAIDKIMQKGGLSNVGKALSYINKHYFSDANGNRGGAPNVAVVLVDGW---PTDKVEEASRQARESGINIFFVTIEGP---DDNEKHNVVEPNFVDKAVCRTNGFFSLPVASWFALRKAVQ-PLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQHNTKADLLNAIKRINYWSGGTSTGAAITYAAEQLFSKSKPNKRKIMIVITDGRSYDDVRA--PALAVHRSGVIAYSIGI 678 HSP 4 Score: 63.1586 bits (152), Expect = 2.212e-9 Identity = 74/344 (21.51%), Postives = 153/344 (44.48%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGV-GQQISEK----ELN----DIASSGEFYN--VKTFTDLEKFNAKTISNEACQIPQ--KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKED--YKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLT 3671
K+D+ +D S S+G F+I+ DF+ + Q +++ +GI + + + + +++ I+KI G + +G A+ + F+ R P V VVL DG + + ++ +A+ GI I V + G +EK E N + + F++ V ++ L K + + C + C A D+ FV+ S++ + F+ + F+ ++T+ + +TRV +++ ++ F + + ++I+ + + G G A+T A S ++ N K +I+I DG D + A + + G+ ++IG+
Sbjct: 341 KVDIAFLMDGSWSIGKRRFKIQKDFLAEVSQVINVGVAGPMMGIIQYGDDPVTEFSLRQYSNSKELKPAIDKIMQKGGLSNVGKALSYINKHYFSDANGNRGGAPNVAVVLVDGWPT--DKVEEASRQARESGINIFFVTIEGPDDNEKHNVVEPNFVDKAVCRTNGFFSLPVASWFALRK-AVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQHNTKADLLNAIKRINYWSGGTSTGAAITYAAEQLFSKSKPNKRKIMIVITDGRSYD--DVRAPALAVHRSGVIAYSIGIA 679 HSP 5 Score: 56.9954 bits (136), Expect = 1.566e-7 Identity = 40/140 (28.57%), Postives = 70/140 (50.00%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKH-TSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
DIA ++D SSS+ G F++ L + F I +TRV + Y + EF F +H T + LN R++ GG S+ G A+ + + +P +I+++++DG + D++ LA + GV Y++ I
Sbjct: 545 DIAFVIDGSSSVGTGNFRTVLQFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQHNTKADLLNAIKRINYWSGGTST--GAAITYAAEQLFSKSKPNKR--KIMIVITDGRSYDDVRAPALA--VHRSGVIAYSIGI 678
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009632.1 (pep scaffold:Pmarinus_7.0:GL479939:9900:31909:-1 gene:ENSPMAG00000008703.1 transcript:ENSPMAT00000009632.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 204.912 bits (520), Expect = 2.475e-53 Identity = 263/1106 (23.78%), Postives = 490/1106 (44.30%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLP-------LGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--TDIAQDACQIPKPKRI----------CSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN---EAVVEEEF-THIYDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXC----------KTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTT-----EFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQ-KDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQ-DECTMKK-LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTICK--------AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF--NRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGK----ALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF---VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL 4082
DL ++D SSS+ + L F ++ L I +VG++ YS +M P +G +K +++ + + + G TF ALR + + F S+GR G K ++LVTDG N P ++++K GI + +G+G + +L M+S + + A D+ T + C + +P++ CS K D+ L+VD SS V + L F + +++ ++G +K R +V ++ +++EF Y D + ++ K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + F +F S++ C T A + T S D+ +VD S+ + + + + LNI + R+ ++ YS + EF + + K+ + AI + + AL Q+FK R V KI+VL TD ++ KD E +K G+ + VG+ K + LA S +++ ++ A EC+ K+ D+ + +D S+S+G NF + L F+ ++V LD+ KD+ R+G+ ++ + P I F + AD +K I + ++ G T G A+ + +GR V K+LV++TDG AND + P + + K GI + SVGVG+ KEL +AS ++ F L+ +++ C + Q+ A D+IF + SA + F M F+ + + V RV +++ +G+ G + +++ ++ G IG AL +A + V++II G + + LL+ G++ F IG+ G DL+ + S L V ++ L ++S + V G N + D++F LDSS+++G + + F+ +++ + + +V VV F + +E+ A+++AV ++ GA ++G AL
Sbjct: 2 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSG--DMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATDFAGLDSIITALTSTVCTVVQPEKTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDPSDE---VKSSGIVLFSVGVGKAD----RKELALMASPPVDKHMFHATNFAGLDSIIMTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGN--MRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSI-ITALTSTVCTVVQEAQTPAPTPPPGPFAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASS-DAVAHYRGLLRAHGVRSFVIGI-GAFQLLDLQRLPSDPIGLHLYAVGGYAQLLGIAGQVTSVI--VRPGGNGALGPRYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQVGGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAAL 1080 HSP 2 Score: 192.586 bits (488), Expect = 1.547e-49 Identity = 232/945 (24.55%), Postives = 421/945 (44.55%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANE---AVVEEEF-THIYDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTT--------PAIKPQTCS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTT-----EFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQ-KDSLSEAEILKGDGVKIIFVGLSK--NSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTM----------KKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTIC--KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILK-------------FNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECST------KQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGE----ALRECKNELLNSTRKE-VPKVIVLVTDGVST 4166
D+ L+VD SS V + L F + +++ ++G +K R +V ++ + +++EF Y D + + K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + F DF S++ C P + CS D+ +VD S+ + + + + L+I + R+ ++ YS + EF + + K+ + AI+ + AL Q+FK R V KI+VL TD ++ KD E +K G+ + VG+ K + K N +D + + IC ++ Q + K D+ + +D S+S+G NF + L F+ ++V L++ KD+ R+G+ ++ + P I F + AD +K I + ++ G T G A+ + +GR V K+LV++TDG AND + P + + K GI + SVGVG+ KEL +AS ++ F L+ T++ + T C K DL ++ S++ F + F+ L + K+ RV +++ FN G Y D K +++I F G AL LFK R K ++++ DGE D+ + + ++ +K GI +F++G+ GK +L +ASP DK +F NF+ L ++S +C V++ T +LD++FA+D S+ + + FV +IV+ + + A +VG+V EF L QF+ + +++AV ++ G IG AL ++ E VP+V+V++T G S+
Sbjct: 2 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATDFAGLDSIITALTSTVCTVVQPEKTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDPSDE---VKSSGIVLFSVGVGKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGN--MRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSI-IMTLAGPTLAL----TECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADK------KKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGV-GKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVCTVVQEAQTPAPTPPPGPFAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASS 920 HSP 3 Score: 160.999 bits (406), Expect = 7.641e-40 Identity = 252/1147 (21.97%), Postives = 474/1147 (41.33%), Query Frame = 3
Query: 984 CNNQQ-LDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLP-------LGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--TDIAQDAC------QIPKPKRICS------AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN---EAVVEEEFTHIYDDILKKVESL----KHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTT-----EFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQ-KDSLSEAEILKGDGVKIIFVGLSK--NSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT------MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK---GR--EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVG--QQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG----DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVF----IGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL--------------DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST-------RKEVPKVIVLVTDGVS 4163
C+++Q DL ++D SSS+ + L F ++ L I +VG++ YS M P +G +K +++ + + + G TF ALR + + F +GR G K ++LVTDG N P ++++K GI + +G+G + +L M+S + + A D+ T + C Q P P + +K D+ L+VD SS V + L F + +++ +G +K R +V ++ +++EF KK+++ K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + F +F S++ + T QT D+ +VD S+ + + + + L+I + R+ ++ YS + EF + + K+ + AI + + AL Q+FK R V KI+VL TD ++ KD E +K G+ + VG+ K + K N +D + + +C ++ Q KLDVI ID S + +F + FV +IVQ L + + R+G+ + + ++ + K+ G AIG + A F++ GR E VP+VLV++T GA+ + ++A G++ +G+G Q + + L Y V + L I+ + + +P A G D++F + SSA + F + F+ + V + RVA+++ G + F+ + + S++ + V +G AL+ + SD + I++ G D + G+++F +G+ ++ ++ VAS AL S+ + + + L I K T++L DL+F +D S+S+ + FVERI+ + +V V F R E L + ++ + I G ++G +L + + T R V + I +++ G S
Sbjct: 200 CSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSG--NMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIIMTLAGPTLALTECSDKQTA----DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDPSDE---VKSSGIVLFSVGVGKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVCTVVQEAQTPAPTPPPGPFAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASSDAVAHYRGLLRAH--GVRSFVIGIGAFQLLDLQRLPSDPIGLHLYAVGGYAQLLG-----IAGQVTSVIVRPGGNGALGPRYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQV--GGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAALSLGYTTYFSQASDAGLQPAQVGILLTGGASADNAAKAAAQL--QASGVRLFVVGVGSGVDQALIQGVASFPYALHSFTVSDYTGLSTVVVTSLVKYIFRPGKMGGTRKLPSDSAAAGRGLDDVDLVFLIDGSTSVEQQTFHLIKKFVERIITNYYYIGGSLRVAVYQFGGAGRLEVPLGNQNDADSLTHTISNIEKVSGPRNLGASLTYVLSNIYKETTTTKLSQRGNVQQSIFIISGGKS 1319
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009633.1 (pep scaffold:Pmarinus_7.0:GL479939:15464:32536:-1 gene:ENSPMAG00000008703.1 transcript:ENSPMAT00000009633.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 190.274 bits (482), Expect = 5.080e-49 Identity = 240/956 (25.10%), Postives = 432/956 (45.19%), Query Frame = 3
Query: 1554 CSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANE---AVVEEEF-THIYDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS------KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP-NPFLRQDFXXXXXXXXXXXXXXCK--------TTPAIKPQTCS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTT-----EFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQ-KDSLSEAEILKGDGVKIIFVGLSKN---------SP----YNFYE-NLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQR----QDECTMKK-LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTIC--KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILK-------------FNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGE----ALRECKNELLNSTRKE-VPKVIVLVTDGVST 4166
CS K D+ L+VD SS V + L F + +++ ++G +K R +V ++ + +++EF Y D + + K++ GFT AL + F + + ++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + LR F + ++ ++ + K P + CS D+ +VD S+ + + + + L+I + R+ ++ YS + EF + + K+ + AI+ + AL Q+FK R V KI+VL TD ++ KD E +K G+ + VG+ K SP + F+ N A S I +E A Q EC+ K+ D+ + +D S+S+G NF + L F+ ++V L++ KD+ R+G+ ++ + P I F + AD +K I + ++ G T G A+ + +GR V K+LV++TDG AND + P + + K GI + SVGVG+ KEL +AS ++ F L+ T+++ C + Q C K DL ++ S++ F + F+ L + K+ RV +++ FN G Y D K +++I F G AL LFK R K ++++ DGE D+ + + ++ +K GI +F++G+ GK +L +ASP DK +F NF+ L ++S +C V++ + +LD++FA+D S+ + + FV +IV+ + + A +VG+V EF L QF+ + +++AV ++ G IG AL ++ E VP+V+V++T G S+
Sbjct: 19 CSDKQTADLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKPQVPFILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDNLLR--FCXDTAIFAVYLEQTVKPIVQLSTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDPSDE---VKSSGIVLFSVGVGKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGN--MRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSI-ITTLTSTVCTVVQ----CSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADK------KKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGV-GKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVCTVVQ---EARPFAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASS 943 HSP 2 Score: 188.734 bits (478), Expect = 1.211e-48 Identity = 269/1124 (23.93%), Postives = 497/1124 (44.22%), Query Frame = 3
Query: 984 CNNQQ-LDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLP-------LGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKK-----TVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKP-------------KRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN---EAVVEEEF-THIYDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS-KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKT-----TPA----------IKPQTCS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTT-----EFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQ-KDSLSEAEILKGDGVKIIFVGLSK--NSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKK-LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQ--KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKF--NRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGK----ALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF---VDNFSNLQRYNAYISSELCDVIKGTNKECSTK---QLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL 4082
C+++Q DL ++D SSS+ + L F ++ L I +VG++ YS +M P +G +K +++ + + + G TF ALR + + F S+GR G K ++LVTDG N P ++++K GI + +G+G + +L M+S P++++ + DT A Q KP CS K D+ L+VD SS V + L F + +++ ++G +K R +V ++ +++EF Y D + ++ K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + F +F S++ C TPA + + CS D+ +VD S+ + + + + LNI + R+ ++ YS + EF + + K+ + AI + + AL Q+FK R V KI+VL TD ++ KD E +K G+ + VG+ K + K N +D + + +C +C+ K+ D+ + +D S+S+G NF + L F+ ++V LD+ KD+ R+G+ ++ + P I F + AD +K I + ++ G T G A+ + +GR V K+LV++TDG AND + P + + K GI + SVGVG+ KEL +AS ++ F L+ +++ C + Q +P A D+IF + SA + F M F+ + + V RV +++ +G+ G + +++ ++ G IG AL +A + V++II G + + LL+ G++ F IG+ G DL+ + S L V ++ L I+ ++ VI C T+ D++F LDSS+++G + + F+ +++ + + +V VV F + +E+ A+++AV ++ GA ++G AL
Sbjct: 19 CSDKQTADLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSG--DMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKPQVPFILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVG-KADRKELALMASP----PVDNLLRFCX-DTAIFAVYLEQTVKPIVQLSTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDPSDE---VKSSGIVLFSVGVGKADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVV-----QCSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGN--MRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSI-ITALTSTVCTVVQEARPF---AKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASS-DAVAHYRGLLRAHGVRSFVIGI-GAFQLLDLQRLPSDPIGLHLYAVGGYAQLLG----IAGQVTSVI--VRSSCVTRGAQYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQVGGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAAL 1102 HSP 3 Score: 169.474 bits (428), Expect = 1.329e-42 Identity = 156/550 (28.36%), Postives = 260/550 (47.27%), Query Frame = 3
Query: 2679 ECTMKK-LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQ-----KPTIC------KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNY--------IDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGT----------------NKECSTKQ-LDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDAR-----KEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDG 4157
EC+ K+ D+ + +D S+S+GP NF + L F+ ++V LD+ KD+ R+G+ ++ ++ + I F + AD +K I ++ G T G A+ + ++GR VP +LV++TDG AND + P + + K GI + SVGVG+ KEL +AS N+ F A + I Q PT+ K DL ++ S++ F + F+ L + K+ RV +++++ GN D G D K +++I+ F G AL LFK R K ++++ DGE D+ + + ++ +K GI +F++G+ GK +L +ASP DK +F NF+ L ++S +C V++ +KECS KQ DL +D SSS+G + L F+ ++V ++I + +VG+V ++ + R KEF + Q+ +K + +A+ R + G T G ALR R V K++VLVTDG
Sbjct: 18 ECSDKQTADLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMR-SPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKPQVPFILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASP-PVDNLLRFCXDTAIFAVYLEQTVKPIVQLSTLAGPTLALTECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYS-GNMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGV-GKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDG 557 HSP 4 Score: 141.739 bits (356), Expect = 3.774e-34 Identity = 223/983 (22.69%), Postives = 416/983 (42.32%), Query Frame = 3
Query: 984 CNNQQ-LDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLP-------LGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--TDIAQDAC------QIPK-----------PKRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN---EAVVEEEFTHIYDDILKKVESL----KHVGGFTRIDKALNLASRSFYLKSKNS-KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTT-----EFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQ-KDSLSEAEILKGDGVKIIFVGLSK--NSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK---GR--EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVG--QQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAA--GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVF----IGKALTSARVLFKSDT-------RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKAL 3725
C+++Q DL ++D SSS+ + L F ++ L I +VG++ YS M P +G +K +++ + + + G TF ALR + + F +GR G K ++LVTDG N P ++++K GI + +G+G + +L M+S + + A D+ T + C Q P P + CS K D+ L+VD SS V + L F + +++ +G +K R +V ++ +++EF KK+++ K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + F +F S++ C QT D+ +VD S+ + + + + L+I + R+ ++ YS + EF + + K+ + AI + + AL Q+FK R V KI+VL TD ++ KD E +K G+ + VG+ K + K N +D + + +C Q+ KLDVI ID S + +F + FV +IVQ L + + R+G+ + + ++ + K+ G AIG + A F++ GR E VP+VLV++T GA+ + ++A G++ +G+G Q + + L Y V + L + S I + + + A D++F + SSA + F + F+ + V + RVA+++ G + F+ + + S++ + V +G AL+ + S R V I++ G + N + A L+ G+++F +G+ ++ ++ VAS AL
Sbjct: 225 CSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSG--NMRSPRIKKEFDMGQYADKKKLKAAIQGTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTICTVVQEAQTPAPVTAATQAVTLPSKECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVVQCSDKQTA----DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDPSDE---VKSSGIVLFSVGVGKADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVCTVV---QEARPFAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAEGGRAGEGVPQVLVIITGGASSDAVAHYRGLLRAH--GVRSFVIGIGAFQLLDLQRLPSDPIGLHLYAVGGYAQLLGIAGQVTS----VIVRSSCVTRGAQYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQV--GGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAALSLGYTTYFSQASDAGLQVRPGPPAQVGILLTGGASADNAA-KAAAQLQASGVRLFVVGVGSGVDQALIQGVASFPYAL 1179 HSP 5 Score: 117.857 bits (294), Expect = 9.272e-27 Identity = 161/784 (20.54%), Postives = 352/784 (44.90%), Query Frame = 3
Query: 927 ETKRTTTSNYIEISTPDQNCNNQQ-LDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLP-------LGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--TDIAQDACQIPKPKRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN---EAVVEEEFTHIYDDILKKVESL----KHVGGFTRIDKALNLASRSFYLKSKNS-KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGG--------DLNTALNRARQVFKIDYRA--DVAKIVVLFTDKLPTNQKDSLSEAE-ILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-------REDVP-KVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS 3149
+T T+ ++ P + C+++Q DL ++D SSS+ + L F ++ L I +VG++ YS M P +G +K +++ + + + + G TF ALR + + F +GR G K ++LVTDG N P ++++K GI + +G+G + +L M+S + + A D+ T + C + + CS K D+ L+VD SS V + L F + +++ ++G +K R +V ++ +++EF KK+++ K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL ++++ P + F +F S++ C +P + D+IF +D S + ++ + +Q L++ R+ ++ + +EF+L + +++++ A+ ++ ++G G L TA ++A RA V +++V+ T + D+++ +L+ GV+ +G+ + + L S + + + + + + + D++ +D S +LG F F+ +++ L + + R+ + +S+ E + + ++++ + G +G A+ F++ R P +V ++LT GA+ ++ +A + + G+++ VGVG + + + +AS
Sbjct: 418 QTPAPVTAATQAVTLPSKECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSG--NMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVVQ----CSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITALTSTVCTVVQEARP---FAKLDVIFAIDGSAGVAAADFTLMKGFVAQIVQGLSVGAAQVRVGLVQLCGKQGSEFKLGQFSSRQEVVAAVGKVSAHGGGRAIGATVQFALATAFSQAE-----GGRAGEGVPQVLVIITGGASS---DAVAHYRGLLRAHGVRSFVIGIGAFQLLDL-QRLPSDPIGLHLYAVGGYAQLLGIAGQVTSVIVRSSCVTRGAQYADIVFYLDSSATLGAAGFVSLKAFLVQVLSYLQVGRYTVRVAVVQVGGAPVAAFQFNSHSERSALVAAVSQLKAVGGARNVGAALSLGYTTYFSQASDAGLQVRPGPPAQVGILLTGGASA--DNAAKAAAQLQASGVRLFVVGVGSGVDQALIQGVAS 1174
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007304.1 (pep scaffold:Pmarinus_7.0:GL477669:89164:98057:1 gene:ENSPMAG00000006585.1 transcript:ENSPMAT00000007304.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 154.451 bits (389), Expect = 5.904e-38 Identity = 266/1214 (21.91%), Postives = 501/1214 (41.27%), Query Frame = 3
Query: 1002 DLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTET---YYPIEDMSKMALFDTTDIAQDAC--------QIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---THIY-DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTTPA-IKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL------RSNGNGGD--------LNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSK----------NSP---YNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNV----------KTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVF--------IGKALTSAR----VLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDK--ALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTK--QLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS---TRK--EVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLK 4322
D++F++D S S+ + + Q +F +++ L + +VG+ YS + + +K + ++ L G T + N L + S+ + + VI++TDG P V K++ MG V IG IKD +L +M+S+ Y ++D S +A + I Q C +I KPK DI I+D S V + + + ++ ++ R +V + + EF TH +D+LK + K GG + A + + S++ + VLLT S + V+ + A L++SGV + +G +KA+ EL ++ + P+ F +++F+ S L + ++ I+ DI+F++D + + + ++ +R L++ ++ LN+ + RI + Y + +T F L+ TK ++ ++ R+N G + A +R RQ F +I VL T +Q ++ AE +KG G + +G ++ +SP + FY N + + + M+ E DV+ +D S ++G +NF+ FV +IV+ LD+ + R+G+ ++ +E +LN ++ D+Q I F G A G A+ + FT + E ++ V+LT +D + + A K G+K+ ++G +Q +LN + S +V +F K+ I PT + D++F++ S E F+ + + VDK++ R+ + + Y E + + ++ +D+T + G+ +G AL + V K + +++ G + ++ A LK DG VF IG G +L + S P+K + +F L + ++ L ++K ++E TK + D++ +D S SIG +Q +F+ IV + + + ++G+ F+ D R EF L F+ + ++ +K + + G T G AL + + +RK VP++ V++TDG S + E + + I I D Q+ SS Y ++DF L+
Sbjct: 36 DIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYADKASLLNYLKTLPYKGGNTKTGLGIEFLMQNHLTDAAG---SRKAQNVPQVVIIITDGKSQDDVAGPAV---KMRNMGASVYAIG-----IKDADISELGQMASSPASKFVYNVDDFSALAGI-SEQILQTVCVTVEEIVTEIRKPKFA-----DIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNG--HAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQDEVE--RRAKALKDSGVTVFALG-ANKANLNELNLIGSDPSANFVFYQRNFRGFLSSLARILQSLLGLIQINIEVIEGERYADIVFLLDDTDNVGSANFEELRKLVIMLIEGLNVGKDKVRIGMAQYGRQPSTTFFLNAHGTKEEVAAGVRSAVFRGGPRANTGSGMRFLQENHFVEQAGSRVRQGFP--------QIAVLLTGS--ASQDNASMGAEAIKGAGGTVFAIGANRARLAELNRVASSPPDNHAFYVN-----NFGFPPMWVCLSDTFSPLFTMLGHLPSWINTEVEKPYADVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLN----THGTKPDVQAAIQLARFTGGRGANTGEAVRSLLQNHFTAQAGSRKDEGYTQIAVILT--GSDSQDVVEQHAGALKQEGVKVFALGA-EQARLPQLNALGSLPSEQHVLYAPNFSELFSSFDRFLKYLRLKIHTGILAGGVSPT--EGYADVVFLVDGSDAVGQENFRHSLGLVSRVVDALDVDKDHIRIGLAQ-----YSGDPRAEFFLSTHAAKKDVTDAIRGIRFKGGRSGNMGAALQHLKDNHFVEAAGSRVKEGFPQIAVLVAGSGSG-DDVLASAQALKNDGTTVFAIG-AGAAKLGELVTAGSDPEKDYVFYQRDFRALSGLSGQLAEGLSSLMKQESEEQCTKVDKADIVLLVDGSWSIGLANFQQMREFLYSIVDGLTVGKDQVRIGMAQFSGDPRTEFFLKTFSSQKDILDYIKTLPYKGGNTMTGRALEFISDNHFTAAAGSRKAQNVPQIAVVITDGKSQDDVEDAAEKLRKLGIAVYAIGIKNADFHELQQIASQPSSKFTYNVVDFSLLR 1196 HSP 2 Score: 53.1434 bits (126), Expect = 5.556e-7 Identity = 41/173 (23.70%), Postives = 78/173 (45.09%), Query Frame = 3
Query: 66 IWSCLIVVACINFEVILALDQRC---IGKP-RDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTL---YDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
+W CL F ++ L + KP D+ +LDSS ++ F+ + Q + + + + RV Y D + EF H + P + A++L R GG + G A+ S L + R +I +IL+ GS++ ++ V+ A ++++GV ++ +
Sbjct: 598 MWVCLSDTFSPLFTMLGHLPSWINTEVEKPYADVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLNTHGTKPDVQAAIQLARFTGGRGANTGEAVRSLLQNHFTAQAGSRKDEGYTQIAVILT-GSDSQDV-VEQHAGALKQEGVKVFALG 768 HSP 3 Score: 51.9878 bits (123), Expect = 1.293e-6 Identity = 46/184 (25.00%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPT-LNFAVRLHRKVGGDSSVLG--RALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI--GXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICV 686
DI ++D S SI N FQ + + + P+ RV Y +TEFL + + LN+ L K G + LG + + L D R + ++++I++DG + D+ V A K++ G ++Y + I + + S + K V +F + G SE ++ +CV
Sbjct: 36 DIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYADKASLLNYLKTLPYKGGNTKTGLGIEFLMQNHLTDAAGS-RKAQNVPQVVIIITDGKSQDD--VAGPAVKMRNMGASVYAIGIKDADISELGQMASSPASKFVYNVDDFSALAGISEQILQTVCV 216
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007296.1 (pep scaffold:Pmarinus_7.0:GL477669:89164:98084:1 gene:ENSPMAG00000006585.1 transcript:ENSPMAT00000007296.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 152.14 bits (383), Expect = 2.715e-37 Identity = 268/1230 (21.79%), Postives = 505/1230 (41.06%), Query Frame = 3
Query: 1002 DLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGAT--------FISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTET---YYPIEDMSKMALFDTTDIAQDAC--------QIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF---THIY-DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKR------LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL------RSNGNGGD--------LNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSK----------NSP---YNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQ--RQDECTMKKL----DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFN----SEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNV-------KTFTDLEKF-----------------NAKTISNEACQIPQKPTICKAAG--DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGV-FIGKALTSARV------LFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDK--ALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTK--QLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS---TRK--EVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLK 4322
D++F++D S S+ + + Q +F +++ L + +VG+ YS + + +K + ++ L G T + N L + S+ + + VI++TDG P V K++ MG V IG IKD +L +M+S+ Y ++D S +A + I Q C +I KPK DI I+D S V + + + ++ ++ R +V + + EF TH +D+LK + K GG + A + + S++ + VLLT S + V+ + A L++SGV + +G +KA+ EL ++ + P+ F +++F+ +SLL DI+F++D + + + ++ +R L++ ++ LN+ + RI + Y + +T F L+ TK ++ ++ R+N G + A +R RQ F +I VL T +Q ++ AE +KG G + +G ++ +SP + FY N + + + F R+DE + DV+ +D S ++G +NF+ FV +IV+ LD+ + R+G+ ++ +E +LN ++ D+Q I F G A G A+ + FT + E ++ V+LT +D + + A K G+K+ ++G +Q +LN + S +V + F+ ++F + + ++P P + G D++F++ S E F+ + + VDK++ R+ + + Y E + + ++ +D+T + G+ F G + + L S T + +++ D + A LK DG VF IG G +L + S P+K + +F L + ++ L ++K ++E TK + D++ +D S SIG +Q +F+ IV + + + ++G+ F+ D R EF L F+ + ++ +K + + G T G AL + + +RK VP++ V++TDG S + E + + I I D Q+ SS Y ++DF L+
Sbjct: 36 DIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYADKASLLNYLKTLPYKGGNTKTGLGIEFLMQNHLTDAAG---SRKAQNVPQVVIIITDGKSQDDVAGPAV---KMRNMGASVYAIG-----IKDADISELGQMASSPASKFVYNVDDFSALAGI-SEQILQTVCVTVEEIVTEIRKPKFA-----DIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNG--HAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQDEVE--RRAKALKDSGVTVFALG-ANKANLNELNLIGSDPSANFVFYQRNFRILQSLLGLIQINIEVIEGVHFFAPAERYADIVFLLDDTDNVGSANFEELRKLVIMLIEGLNVGKDKVRIGMAQYGRQPSTTFFLNAHGTKEEVAAGVRSAVFRGGPRANTGSGMRFLQENHFVEQAGSRVRQGFP--------QIAVLLTGS--ASQDNASMGAEAIKGAGGTVFAIGANRARLAELNRVASSPPDNHAFYVN---------------NFGDLSGLYTQLMGGFNTLLRRDEEEEEVEKPYADVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLN----THGTKPDVQAAIQLARFTGGRGANTGEAVRSLLQNHFTAQAGSRKDEGYTQIAVILT--GSDSQDVVEQHAGALKQEGVKVFALGA-EQARLPQLNALGSLPSEQHVLYAPNFSELFSSFDRFLKYLRLKIHTGILAGASQSVIVIYWGVKVPM-PCVSPTEGYADVVFLVDGSDAVGQENFRHSLGLVSRVVDALDVDKDHIRIGLAQ-----YSGDPRAEFFLSTHAAKKDVTDAIRGIRFKGNGSSRPKYPGKMVPLCHSVTGPDKDNIAVLVAGSGSGD--DVLASAQALKNDGTTVFAIG-AGAAKLGELVTAGSDPEKDYVFYQRDFRALSGLSGQLAEGLSSLMKQESEEQCTKVDKADIVLLVDGSWSIGLANFQQMREFLYSIVDGLTVGKDQVRIGMAQFSGDPRTEFFLKTFSSQKDILDYIKTLPYKGGNTMTGRALEFISDNHFTAAAGSRKAQNVPQIAVVITDGKSQDDVEDAAEKLRKLGIAVYAIGIKNADFHELQQIASQPSSKFTYNVVDFSLLR 1202 HSP 2 Score: 98.9821 bits (245), Expect = 5.312e-21 Identity = 157/708 (22.18%), Postives = 308/708 (43.50%), Query Frame = 3
Query: 2097 AIKPQTCS--SNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTAL-------NRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE----FYN-----VKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTST-NGVFIG--KALTSARVLFKSDTR---------KNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS--PD-KALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQ--LDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDG-ATDIGEALR 4085
A + CS S DI+F++D S N + +RS + T ++ L+++ R+ + YS + TEF ++ K + +K L G G+ T L N +V ++V++ TD +Q D A ++ G + +G+ K++ + +AS +S + N++ + + +C ++ E K D++ +D S ++G F+ V +I LD+ D+ RIG+ +N + ++++ D+ K I + F G G I A + LFT + E ++ V+LT A+ + + A K G+ + ++G + + ELN I S FY +++ L + N + I P + D++F++ + N S F+++ + L + V K+ R+ + ++ R + F + N + E++ + VF G +A T + + F + + + +++ G + N + A +K G VF IG + ++L VAS PD A +V+NF +L + +++ +E ++ D++F LDSS ++G ++ FV +IV +D++A+ +VG+ ++ + R EF L+ K + +A++ F G + GEA+R
Sbjct: 23 AAQKTACSKASVADIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYADKASLLNYLKTLPYKG--GNTKTGLGIEFLMQNHLTDAAGSRKAQNVPQVVIIITDG--KSQDDVAGPAVKMRNMGASVYAIGI-KDADISELGQMASSPASKFVYNVDDFSALAGISEQILQTVCVTVEEIVTEIRKPKFADIVFIMDGSDNVGKAGFEDMRKVVYKIASILDVAPDQNRIGVVQYNDNGHAEFLLNTHSSKEDVLKNIRQFKFRGGIANTGAGIRFAEERLFTEQAGSRKAEGFAQIAVLLTGSASQ--DEVERRAKALKDSGVTVFALGA-NKANLNELNLIGSDPSANFVFYQRNFRILQSLLGLIQINIEVIEGVHFFAP-----AERYADIVFLLDDTDNVGSANFEELRKLVIMLIEGLNVGKDKVRIGMAQYGR--QPSTTF---FLNAHGTKEEVAAGVRSAVFRGGPRANTGSGMRFLQENHFVEQAGSRVRQGFPQIAVLLTGSASQDNASM-GAEAIKGAGGTVFAIG-ANRARLAELNRVASSPPDNHAFYVNNFGDLSGLYTQLMGGFNTLLRRDEEEEEVEKPYADVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLNTHGTKPDVQAAIQLARFTGGRGANTGEAVR 710 HSP 3 Score: 52.373 bits (124), Expect = 9.728e-7 Identity = 36/145 (24.83%), Postives = 69/145 (47.59%), Query Frame = 3
Query: 141 KP-RDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTL---YDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
KP D+ +LDSS ++ F+ + Q + + + + RV Y D + EF H + P + A++L R GG + G A+ S L + R +I +IL+ GS++ ++ V+ A ++++GV ++ +
Sbjct: 621 KPYADVVFLLDSSDAVGRKNFEGMKTFVGQIVEYLDVDASKVRVGLAQYSDNPRNEFFLNTHGTKPDVQAAIQLARFTGGRGANTGEAVRSLLQNHFTAQAGSRKDEGYTQIAVILT-GSDSQDV-VEQHAGALKQEGVKVFALG 763 HSP 4 Score: 51.9878 bits (123), Expect = 1.191e-6 Identity = 46/184 (25.00%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPT-LNFAVRLHRKVGGDSSVLG--RALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI--GXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICV 686
DI ++D S SI N FQ + + + P+ RV Y +TEFL + + LN+ L K G + LG + + L D R + ++++I++DG + D+ V A K++ G ++Y + I + + S + K V +F + G SE ++ +CV
Sbjct: 36 DIVFLIDGSWSIGNINFQQMRSFLYTLVEGLDVAPDKVRVGMAQYSGDARTEFLMNTYADKASLLNYLKTLPYKGGNTKTGLGIEFLMQNHLTDAAGS-RKAQNVPQVVIIITDGKSQDD--VAGPAVKMRNMGASVYAIGIKDADISELGQMASSPASKFVYNVDDFSALAGISEQILQTVCV 216
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009635.1 (pep scaffold:Pmarinus_7.0:GL479939:22902:31906:-1 gene:ENSPMAG00000008703.1 transcript:ENSPMAT00000009635.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 116.701 bits (291), Expect = 8.435e-27 Identity = 151/670 (22.54%), Postives = 294/670 (43.88%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGY-GDT----LKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFP--EFQTMRGDSENLVSVICVV----KYTKP--PSRETISIPDITSSSATVRNXXXXXXXXXXXXI-------------------IRTTIPDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIPFETKRTTTSNYIEISTPDQNCNNQQ-LDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLP-------LGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--TDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN---EAVVEEEFTHIYDDILKKVESL----KHVGGFTRIDKALNLASRSFYLKSKNS-KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILS 2006
D+ +++D SSS+ G F L + + + I + RV + Y GD +K EF ++ L A+ L K + G AL TL + RP + +IL++++DG D+ VKD + +++ G+ +++V +G L L S + MF +F + L S +C V K P + + +++P + + R + + RTT PD S + + ++ +P R ++S+ C+++Q DL ++D SSS+ + L F ++ L I +VG++ YS M P +G +K +++ + + + + G TF ALR + + F +GR G K ++LVTDG N P ++++K GI + +G+G + +L M+S + + A D+ T + C + + + + D+ L+VD SS V + L F + +++ ++G +K R +V ++ +++EF KK+++ K++ GFT AL + F + + +++VL+T+G + ++V+ + +++ SG+ + ++G+G KADR+EL +++
Sbjct: 1 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAI-LGTKYMRGFTFTGAALRHTLQLFKSQGRPG--VGKILVLVTDGEANDK--VKDPSDEVKSSGIVLFSVGVGKADRKELALMASPPVDKHMFHATDFAGLDSIITALTSTVCTVVQEAKVAVPVTAATQAVALPSKGAGGSVQRRLALRRTFSIFPKLAGFWGRHCLNNEHRADCKTYRTTFPDPCWP------------HSGTHRVQRQADSRPVP-AGGRLQQRGAPQLSS---ECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSG--NMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVG-KADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVVQEAQTPAPTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGVG-KADRKELALMA 640 HSP 2 Score: 114.775 bits (286), Expect = 2.685e-26 Identity = 92/331 (27.79%), Postives = 152/331 (45.92%), Query Frame = 3
Query: 3234 PQKPTIC--KAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILK-------------FNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDAR-----KEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDG 4157
PQ + C K DL ++ S++ F + F+ L + K+ RV +++ FN G Y D K +++I F G AL LFK R K ++++ DGE D+ + + ++ +K GI +F++G+ GK +L +ASP DK +F NF+ L ++S +C V++ T DL +D SSS+G + L F+ ++V +DI + +VG+V ++ + R KEF + Q+ +K + +A+ R + G T G ALR R V K++VLVTDG
Sbjct: 284 PQLSSECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADK------KKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGV-GKADRKELALMASPPVDKHMFHATNFAGLDSIITTLTSTVCTVVQEAQTPAPTA--DLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDG 603 HSP 3 Score: 107.457 bits (267), Expect = 5.753e-24 Identity = 99/372 (26.61%), Postives = 173/372 (46.51%), Query Frame = 3
Query: 2667 QRQDECTMKK-LDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVP-IDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILK-------------FNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVA 3707
Q EC+ K+ D+ + +D S+S+G NF + L F+ ++V L++ KD+ R+G+ ++ + P I F + AD +K I + ++ G T G A+ + +GR V K+LV++TDG AND + P + + K GI + SVGVG+ KEL +AS ++ F L+ T+++ C + Q+ DL ++ S++ F + F+ L + K+ RV +++ FN G Y D K +++I F G AL LFK R K ++++ DGE D+ + + ++ +K GI +F++G+ GK +L +A
Sbjct: 285 QLSSECSDKQTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLNIGKDKVRVGMVQYSGN--MRSPRIKKEFNMGQYADKKKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATNFAGLDSI-ITTLTSTVCTVVQEAQTPAPTADLYLLVDGSSSVGRRNFHLVLRFLAKLVDKLDIGKDKVRVGMVQYSGNMRSPRIKKEFNMGQYADK------KKLKAAILRTKYMRGFTFTGAALRHTLQLFKRQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGV-GKADRKELALMA 640 HSP 4 Score: 73.1738 bits (178), Expect = 3.029e-13 Identity = 57/195 (29.23%), Postives = 101/195 (51.79%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNF---ENADMQK---EINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAKTISNEACQIPQKPTIC 3254
D+ + +D S+S+GP NF + L F+ ++V LD+ KD+ R+G+ ++ ++ + I F + AD +K I ++ G T G A+ + ++GR V K+LV++TDG AND + P + + K GI + SVGVG+ KEL +AS ++ F L+ +++ C + Q+ +
Sbjct: 1 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMR-SPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKILVLVTDGEANDKVKDP---SDEVKSSGIVLFSVGVGKA-DRKELALMASPPVDKHMFHATDFAGLDSI-ITALTSTVCTVVQEAKVA 189 HSP 5 Score: 67.3958 bits (163), Expect = 1.908e-11 Identity = 39/114 (34.21%), Postives = 61/114 (53.51%), Query Frame = 3
Query: 3831 DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDAR-----KEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDG 4157
DL +D SSS+GP + L F+ ++V +DI + +VG+V ++ D R KEF + Q+ +K + +A+ + G T G ALR + R V K++VLVTDG
Sbjct: 1 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKILVLVTDG 114 HSP 6 Score: 56.6102 bits (135), Expect = 3.334e-8 Identity = 48/187 (25.67%), Postives = 89/187 (47.59%), Query Frame = 3
Query: 3267 DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYI----DSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP--DKALF-VDNFSNLQRYNAYISSELCDVIK 3797
DL ++ S++ F + F+ L + K+ RV +++++ R I D G D + +++I F G AL LFKS R K ++++ DGE D+ + + ++ +K GI +F++G+ GK +L +ASP DK +F +F+ L ++S +C V++
Sbjct: 1 DLYLLVDGSSSVGPGNFHLVLKFLAKLVDKLDIGKDKVRVGMVQYSGDMRSPKIKKEFDMGQYADKEKLKAAILGTKYMRGFTFTGAALRHTLQLFKSQGRPGVGKILVLVTDGEANDKVK--DPSDEVKSSGIVLFSVGV-GKADRKELALMASPPVDKHMFHATDFAGLDSIITALTSTVCTVVQ 184
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO40384 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7E3]) HSP 1 Score: 117.472 bits (293), Expect = 3.888e-26 Identity = 234/1107 (21.14%), Postives = 468/1107 (42.28%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQV--GVLIYSDILEMSLPLGSKQ-NKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQF--ELGINKTRFSLVSFANEAVVEEEFTHIYDD--ILKKVESLKHVGGFTRIDKALNLASRSFYLKS--KNSKRLVVL---LTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSL--NXXXXXXXXXXXDYSDETTTEFRLDEAF-TKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID-YRADVAKIVVLFTD-KLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNS---------------EVYL-NVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG--REDVPKVLVVLTDGANDIGESPQISAVKAKLG-GIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE---SSIEDMTTSTNGVFIGKALTSARVLFKSD-TRKNATKTVIIIIDGEITDRNETFEEAN-LLKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYA--MISAVKRIIFDDGATDIGEALRECKNELLN-STRKEVPKVIVLVTDGVSTGGLS 4178
++DL+F + AS+S D LA + + S++ ++I+ L K ++ + ++ + + G I A + + F +G R A+K V+++ D + + +L + + VI + +G DQL +S T S++ I Q Q K +LD+A+ V ++ + + AK ++ G+ R S + +++ A+ + F YD+ + + +ES + G + +DKAL S +N+ +++VL +T+GLS ++ AA LQ+ + + I +G+ EL+ + + R++ V + ++ DI+F + ++ +++++ SLM +++S+ T S+I Y D T + + ++ D+ +I ++ ++ AL A+QVF+ + R + +KI+V+ D P + ++ +LK GV+II V + N E + K I + I + I A K+ + ++++ + ++ + + + D + R++ R D+ + + S E ++ V ++ + ++ +I+ +P +AI + A T R + KVLV++TD + ES S K L IKII V VG ++ ++EL I + + V + A I N+A P K + + DL+F + +S+ + S +K M + S+ L + + R ++ ++ GF ++Y N E + I + +T G I +AL++A +F+ R A K +++I D E A+ L + +K+ + + + S DL++ + +PD + S + I ++ + +E S ++DL FAL ++S+ P ++Q D V+ I+ I+ + V R FG D + ++ A+ R+ G +D+G+AL E N R KV+V++TD S+ G++
Sbjct: 490 EIDLVFAISASAS---DPDTTLALMKKTAQHIVDTYGTSKIRYSLVIFGSQTSTQLSFDRKMPDRDSLSSMIAGMPRIPGRPAIDQAFLEADKLFQLQGTRPNARKVVVMMVDQRSAATDDDLRRSSRRLHQRKVDVIPVAIGSESDPDQLLHTTSDNTNLIKAAKSELPNSLANQIMQKIVQGRK-----VPELDLAVAVSATAT----DADTTFMLAKNALKSIMDRYGVGAIRLSFIVYSDMALKKFTFQDKYDEEELKRSIESAMRMDGGSALDKALEAGSEVLQGPGARENAHKVLVLITDMTSGLSDAEIEA--AAKPLQQKQIAVVPIAVGNDVTTRELEKTTPY-----RENVMTAYRAENPEVLGAKIIDKILQAIRRVPKMDIVFAMSATSAQSDQMF----SLMKNSIKSIIDMHGTTDIHYSVILYGDTATIKRDFGASVPSEEDLQRSIDAMQPLLGRALVDKALQLAQQVFEGEGVRPNASKILVVMMDMSSPLSVAYLKAKTRLLKDSGVRIIAVAAGQAVDANKLEQVLPKGGVISAPEGTSPGNLSKSIMDTILKAPKKPE-------INLMFMVSATSQRASQIYTLMKDTMKRVIDKYGTR-DQVHYSVIVYGSSPTTEITFQDSYNIAEAHIITVVLEQSVSPVALKSDIDALPRDSSGSAITRTLDEALKQFATYDGVRPEASKVLVIITDKRSGASESEIRSKAKPLLDKNIKIIPVAVGGEVDKRELEAITPNNDDIIVVPPEEKATALATKIMNKALT-PMKVVLPEL--DLVFAISASSREASIAYKLMKATLISI--LDQYGRGDFRYGVITYSNYATRRKGFGDNYPNIEDVKTLIRYLPPNTRGSAINRALSAADAMFRGPFVRPTADKILVLITDNASGMTVEEIRAASKPLTDNKVKIIGVAMGNQASVPDLQAASGNPDNVMQAKLSSKPNKLGNKIMQKVLE------REPSIAEIDLAFALSATSA--PQHFQVMKDTVKSIIEKYGIDRIHLSLIVYGDLPYTRVSFGQDIPDARTLKNLVEALSRV---SGGSDLGKALAEATKVFRNDDVRPNAKKVLVVITDSKSSYGVA 1549 HSP 2 Score: 106.686 bits (265), Expect = 7.167e-23 Identity = 204/1006 (20.28%), Postives = 419/1006 (41.65%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLALYWDQELAFTNNVIKSL-----QIDIKFSQVGVLIYSDILEMSLPLG-SKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNK-------MSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQ--VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--------THIYDDILKK----------VESLKHVGGFTRIDKALNLASRSFY----LKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKP-QTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTK-RDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY-RADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKE-INKIPFIEGNTAIGWAIGNATDYLFTKG-REDVPKVLVVLTDGANDIGESPQISAVKA-KLGGIKIISVGVGQQISEKELNDIA-SSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESS---IEDMTTSTNGVFIGKALTSARVLFK-SDTRKNATKTVIIIIDGEITDRNETFEEANL-LKKDGIKVFTIGLTGKTSESDLKSVA-SPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSS 3857
++D++F + A+S+ + DQ + N IKS+ DI +S V++Y D + G S ++ ++Q+ + ++ L G + AL+ + + F +G R A K ++++ D + + + LK G+R+I + G +V ++L + +S+ E P ++SK ++ DT + PK+ ++++ +V +SQ Q++ + + KR I+++ ++ +S++ + + E F HI +L++ +++L + I + L+ A + F ++ + SK LV++ + A L + + I + +G + D+ EL+ ++ PN D + + + A+ P + D++F + S+ + + +++ +++ L + YS+ T + + D+ I+ L N G +N AL+ A +F+ + R KI+VL TD + + A + L + VKII V + + +L + + K+ ++ N I +R E ++ ++D+ + S + P++FQ+ D V I++ + DR + + + Y V + +A K + + + G + +G A+ AT R + KVLVV+TD + G + + A + + GI++++V +G EL I G+ + D + +S + + P + DL F + + ++ + + M D +KS+ +T E R ++ + F + N S I+ + + +G + KAL + LF+ TR K +++I D + T E A L K G+ V I + + +L A +P L + A ++ + D+I E +DL FA+ SS
Sbjct: 860 KMDIVFAMSATSAQS---DQMFSLMKNSIKSIIDMHGTTDIHYS---VILYGDTATIKRDFGASVPSEEDLQRSIDAMQPLLGRALVDKALQLAQQVFEGEGVRPNASKILVVMMDMSSPLSVAYLKAKTRLLKDSGVRIIAVAAGQAVDANKLEQVLPKGGVISAPEGTSP-GNLSK-SIMDTI--------LKAPKK---PEINLMFMVSATSQRASQIYTLMKDTM--KRVIDKYGTR-DQVHYSVIVYGSSPTTEITFQDSYNIAEAHIITVVLEQSVSPVALKSDIDALPRDSSGSAITRTLDEALKQFATYDGVRPEASKVLVIITDKRSGASESEIRSKAKPLLDKNIKIIPVAVGGEVDKRELEAIT--PN---NDDIIVVPPEEKATALATKIMNKALTPMKVVLPELDLVFAISASSREASIAYKLMKATLISILDQYGRGDFRYGVIT--YSNYATRRKGFGDNYPNIEDVKTLIRYLPPNTRGSAINRALSAADAMFRGPFVRPTADKILVLITDNASGMTVEEIRAASKPLTDNKVKIIGVAMGNQASV---PDLQAASGNPDNVMQAKLSSKPNKLGNKIMQKVLER--EPSIAEIDLAFAL--SATSAPQHFQVMKDTVKSIIEKYGI--DRIHLSLIVYGDLPYTRVSFGQDIPDARTLKNLVEALSRVSGGSDLGKALAEATKVFRNDDVRPNAKKVLVVITDSKSSYGVASLVKAAEPLEHAGIRVLAVAIGADADASELRSIVQHQGDVIKASSSEDPSGLADRLVSAMLKETRKIPKV-----DLGFAVSAGSSNSDATLRHMKDIIKSI--VTRYGAERMRYGLMSYGDDVTTTIFFTDGITNPNSLLPFIDILQKTPSGSSLEKALVGGKKLFEHGGTRPGVDKFLVVITDRKSTGDAAQTENAKADLAKAGVNVLVIAVGSEVDHKELNETATTPSHKL----VTAPGESPAEVADRVVDII--NKDELDFTAVDLGFAISSS 1814 HSP 3 Score: 97.0561 bits (240), Expect = 5.405e-20 Identity = 182/885 (20.56%), Postives = 377/885 (42.60%), Query Frame = 3
Query: 1197 FVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD----IAQDACQIPKP--KRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY--DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSM---ERVQTIQAAYDLQESGVDIATIGLGDKADREELKILS----------TFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKST-DNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAF-TKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGD-GVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENA-DMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVV------LTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVK-TFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKL--------TVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT--RKNATKTVIIIIDGEITDR-NETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPD 3716
V + G + + NAL + F ++ R A+K +I+ D + + L K+G+RVIV+ + + + ++ +++ D + + D IA + K KRI S + IA+ + + + + + + I+Q+ GI K +S++ F + V + F D+ + + V G I KAL A + ++ ++ R+VV++ + S+ ++ ++AA ++++ GV + +GLG D EL +++ T P+P RQ + + + P +KP D++F V S+ N + + I+ ++ T +IS I + D+TT+ + F + D+ I + ++ AL++AR F+ R++ KI+V+ DK N + + +A D VK+I V + +++ +L + N + ++ K+ E + ++ D++ + + ++F++ + V +V + R G+ F + V V F +A D+ K I+ + +G +G A+ A R+ K+L+ TD A+ +S +++++ G+KII + VG +I +++ + SG+ N++ + TD + + + ++ + K + D+ F + +S+ + F +M + +K++ + T+V T +++ ++G + +G + I+ +T + G I AL + FK T R N KT+II+ D D ++ ++ DGI+V +G+ +LK VA D
Sbjct: 4131 LVDAMPRRSGGSALGNALENARTMFHTEARPNAEKVLIIFIDQRPSDDVGVAINSSIALDKLGVRVIVVAMQMPNVPVEIEQVAP--------DRGSIIRANNNDKPQAIADKILNLSKKDVKRIPSIDMVIAISATVARPDRTFKSMKNTI--RSIIDQY--GIAKIHYSVIVFGDRPVTKITFQDRLSPSDLKSYIRTFARVNGGPSISKALQQARYEWEQDARPDAHRVVVIIVDNKSVGDSGNIKKLRAAKEMEKDGVIVIPVGLGRDFDENELHLITPYKENSIARVTLPDP--RQ-----LARDIMDKSLMAISKPLVKPM------DVLFAVSSSSPQNLLKAKELIKRMIDT------YGMDKLKISYIGFGDKTTSPISFKDKFPSAEDLKSRIDVISLVPGRPNVLEALDKARNEFETGGRSNAKKILVIVVDKRSPNTNEQIKDAVTPLADRDVKVIVVTVGEDADPQQGTSL------TGDISAVIKADNSSEPDQLVQKIVKEGSKEVSPVRDADIVFALSATGQQRDQSFRMLQEAVKGLVARYGI--GSVRYGVIVFGNTVSTAVDFGDQFNSASDLMKAISNLERKDGGPRLGLALDRAKRQFSNTNRKGAKKILIFTLDQKPTTDEASIRSKSDELNSM-----GVKIIPLLVGSEIGPQDVKPV--SGDTKNIEVSVTDPPDHVTDKLYKQIGEAVKEESQPKPSLDIGFAISASSADSDANFAKMKNIIKAVLDIYGTEGVHYTIVTFGQTAKTVVQNSKGAELRAGLPTLATELKRLIDRITKNPEGTSIVSALNRIKQSFKEATSSRPNTKKTLIIMTDSNHEDGVDDVIIAMEGVEDDGIQVLYVGVDDSVDLEELKIVAPDD 4969 HSP 4 Score: 97.0561 bits (240), Expect = 5.590e-20 Identity = 180/928 (19.40%), Postives = 382/928 (41.16%), Query Frame = 3
Query: 1188 IQKFVSKLELLEGATFISNALRRSGEEFISKG-REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSS--TETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY---DDILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQ-TIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQD----FXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDS---LSEAEILKGDGVKIIFVGLSKNSPYNFYEN-LAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENA-DMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLG--GIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAA---------GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSG--FKEDY---KNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGE-ITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQ--LDLMFALDSSSS 3863
+++ + L++ +G+ + ALR + + F G R A+ ++++TD + A L IR+I +GLG + K +L M+ + + +D L +I Q PKR ++DIA+ + + K+ + + LG R+ L+ F + A E F+ + DD L V+++ G + + AL A F+ +++ N+++++++ + + V I ++ L + GV + + + E++ ++ +R + + + K K P+I D++ + + + + +++ + + + I++ + + + D T+ + + D+ I+ G ++ AL +AR ++ D R D ++VV+ D + L A+ ++ DGV +I VGL + +F EN L + + Q+ I D + +K +DV+ + S+ P+N + + R++ + D+ +I F + + F +A D++ I+ I + G + A+ A + T GR + K+LV++ D + + QI L +K+I V VG+ ++ + D+ S+E Q+ QK I K D++F + ++ + + F+ + + +K L + + R ++ F GN + + F + + + +I ++ G +G AL A+ F + RK A K +I +D + TD +++ L G+K+ + + + D+K V+ K + V ++ +++ +L I KE S + LD+ FA+ +SS+
Sbjct: 3942 MKRLIDGLQIEQGSPSLGAALREARDMFKMDGVRPHARHVLVVITDSSSGLTDQDISSAARPLHLNEIRLIPVGLGGLIGKKELESMTPVKADVIHVAKDTEPRYL--GKEIMSRVVQALIPKR----QMDIAIAIGSGGPNGDATFTQAKEITKKLLARNPLG--AARYGLIVFGDAAFTETTFSREFPSQDDFLALVDAMPRRSGGSALGNALENARTMFHTEARPNAEKVLIIFIDQRPSDDVGVAINSSIALDKLGVRVIVVAMQMPNVPVEIEQVAPDRGSIIRANNNDKPQAIADKILNLSKKDVKRIPSI---------DMVIAISATVARPDRTFKSMKNTIRSIIDQYGIAKIHYSVIV--FGDRPVTKITFQDRLSPSDLKSYIRTFARVNGGPSISKALQQARYEWEQDARPDAHRVVVIIVDNKSVGDSGNIKKLRAAKEMEKDGVIVIPVGLGR----DFDENELHLITPYKENSIARVTLPDPRQLARDIMDKSLMAISKPLVKPMDVLFAVSSSS---PQNLLKAKELIKRMIDTYGM--DKLKISYIGFGDKTTSPISFKDKFPSAEDLKSRIDVISLVPGRPNVLEALDKARNEFETGGRSNAKKILVIVVDKRSP-NTNEQIKDAVTPLADRDVKVIVVTVGEDADPQQGTSLTG-----------DISAVIKADNSSEPDQLVQK--IVKEGSKEVSPVRDADIVFALSATGQQRDQSFRMLQEAVKGLVARYGIG--SVRYGVIVF--GNTVSTAVDFGDQFNSASDLMKAISNLERKDGGPRLGLALDRAKRQFSNTNRKGAKKILIFTLDQKPTTDEASIRSKSDELNSMGVKIIPLLVGSEIGPQDVKPVSGDTKNIEV----SVTDPPDHVTDKLYKQIGEAVKEESQPKPSLDIGFAISASSA 4819 HSP 5 Score: 94.7449 bits (234), Expect = 2.670e-19 Identity = 224/1100 (20.36%), Postives = 452/1100 (41.09%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQV--GVLIYSDILEMSLPLG-SKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD---------IAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFE-LGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKH-VGGFTRID--KALNLASRSFY------LKSKNSKRLVVLLTNGLS-MERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKP---QTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY-RADVAKIVVLFTDKL-PTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAF--KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENAD-MQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKL-----GGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQ-IPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENT---RVAILKFNRGNYIDSGFKEDYKNFESS---IEDMTTSTNGVFIGKALTSARVLFKSD-TRKNATKTVIIIIDGE--ITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLD-QFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTD 4154
++D+ F + A+++ + + L F ++ S+ + S++ G++ Y D + G S+QN +Q+ V L G + + AL G F S R A+K ++++TD S AE L+ + +I + +G V +L P+ + + TTD I + A + P + K+D+ + +S+ + L I F+ +K R+S+ F + ++ T +DD L V+ LK V R+D AL+ + F +++++++VL+T+ S + + I+ + L + GV I +G+GD+ ++ L+ +T P L+++ K + T IK Q D++F + +T + + + ++ ++L+ + ++ + + S E T RL++ F + I + L+ ++R R +F RA K++VLFTDK +N D ++ A+ L GV +I +GL E + S + + + +++ ICD + R + + +LD++ + + + F + I+ + R G+ F + + + +N+ + + ++K ++ + G + A+ A K+LV+L D +D + +K + G+K++ V VG EL S + KT D + N+A + I + P + D++F + +SA + FK + D +K +VD T A+L F F + E+ I+ + +G AL AR +FK D R +A +++I D +TD++ + A L + I++ +GL G + +L+S+ +P KA + + + Y+ E+ + +Q+D+ A+ S G + + ++++ + A + G++ F A E +F + ++ V + G + +G AL + R KV+++ D
Sbjct: 3136 KMDIAFAISANATSS---EDRLGFIRQIVSSIIMRYGTSKINYGLVTYGDSPTTRINFGDSEQNSDMLQRVVDTLPHSSGGSNLEKALSTVGLMFES-ARADARKVLVIITDRPSTSQADAEAKAAETLRNKNVEIIAVAVGAEVQPGKLR---------PLLPHGRNLINATTDERPDKKAEEIIKQAAKDP----LEDNKVDVGFAISATSKNADSTF---KLMKDALITIFDKYHPDKIRYSVAVFGSRV---QKITS-FDDALPSVDELKRSVEQAARLDGDPALDKVLKDFLEIFKGPRARRDARKVLVLITDKDSGLRKKDIIENSKPLNDLGVVIIPVGVGDEVKKDFLE--TTTP---LKKNVIHIPRTESKEDLA-SRITDKIKNGLYQEHVPEVDLVFAMSAATADADVNFMHMKDVLLSIIDKYSMGKIRYSVLSYGASQEVWT--RLNDDFPDSQLKSVINSISRPFGEPSLHKVMDRVRNLFTSGQPRALAKKVLVLFTDKRSSSNPDDVVNSAKALADAGVHVIAIGLGS-------EASRPELSVSTPDAGDVMTLTRDERPEDICDRIFDRIRSRKPVVPQLDLVFAVSATAPNADDTFARMKRMIGGIITKYG--RKYIRYGLIVFGNSAQTVISLRNNYPDEESLKKALSISSRMSGIPDVVEALKTAKSMFEIDKGNGRAKILVILVDKRSD----NSLDDIKEQASFLIRSGVKVVPVAVGSLADAGELAGSTDSKDKVITKTNMDDPDALGGEVMNKALKAIKETPDV-----DVLFALSTSAIGAPQTFKLVQDTIK-----YIVDTYGTSKIHYAVLVFGAAPKRSILFTDIMVTPEAMKRLIDGLQIEQGSPSLGAALREARDMFKMDGVRPHARHVLVVITDSSSGLTDQDIS-SAARPLHLNEIRLIPVGLGGLIGKKELESM-TPVKADVIHVAKDTEPR--YLGKEIMSRV--VQALIPKRQMDIAIAIGSGGPNGDATFTQAKEITKKLLARNPLGA--ARYGLIVFGDAAFTETTFSREFPSQDDFLALVDAMPRRSGGSALGNALENARTMFHTEARPNAEKVLIIFID 4172 HSP 6 Score: 92.8189 bits (229), Expect = 1.202e-18 Identity = 136/654 (20.80%), Postives = 275/654 (42.05%), Query Frame = 3
Query: 2262 YSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGD-LNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKD-SLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQ--RQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKA----KLGGIKIISVGVGQQISEKELNDIASSGEFYNVK-TFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE---SSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEA--NLLKKD-GIKVFTIG-----LTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECS-TKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARK--EFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTD 4154
+ D TE F +D ++A+ +GG L AL AR +F + R + K++++F D+ P++ +++ + L GV++I V + N+ + + + + I N I D ++D + +D+++ I + + F+ + + I+ + K + F + +D++ I + G +I A+ A R D +V+V++ D + +G+S I ++A + G+ +I VG+G+ E EL+ I E + T D + A+ I +++ KP + D++F + SS+ + + K++ +K + +DK +++ + F FK+ + + E S I+ ++ + +AL AR F++ R NA K ++I++D + NE ++A L +D + V T+G G + D+ +V D + D + ++K +KE S + D++FAL ++ ++ + V+ +V I + + GV+ F +FG DQF ++ A+ + DG +G AL K + N+ RK K+++ D
Sbjct: 4108 FGDAAFTETTFSREFPSQDDFLALVDAMPRRSGGSALGNALENARTMFHTEARPNAEKVLIIFIDQRPSDDVGVAINSSIALDKLGVRVIVVAMQ-------MPNVPVEIEQVAPDRGSIIRANNNDKPQAIADKILNLSKKDVKRIPSIDMVIAISATVARPDRTFKSMKNTIRSIIDQYGIAK--IHYSVIVFGDRPVTKITFQDRLSPSDLKSYIRTFARVNGGPSISKALQQARYEWEQDARPDAHRVVVIIVDNKS-VGDSGNIKKLRAAKEMEKDGVIVIPVGLGRDFDENELHLITPYKENSIARVTLPDPRQL-ARDIMDKSLMAISKPLVKPM--DVLFAVSSSSPQNLLKAKEL---IKRMIDTYGMDK--LKISYIGFGDKTTSPISFKDKFPSAEDLKSRIDVISLVPGRPNVLEALDKARNEFETGGRSNAKKILVIVVDKRSPNTNEQIKDAVTPLADRDVKVIVVTVGEDADPQQGTSLTGDISAVIKADNSSEPDQL-------------VQKIVKEGSKEVSPVRDADIVFALSATGQQRDQSFRMLQEAVKGLVARYGIGS--VRYGVIVFGNTVSTAVDFG-DQFNSASDLMKAISNLERKDGGPRLGLALDRAKRQFSNTNRKGAKKILIFTLD 4727 HSP 7 Score: 90.5077 bits (223), Expect = 5.986e-18 Identity = 186/920 (20.22%), Postives = 384/920 (41.74%), Query Frame = 3
Query: 1176 NKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY---DDILKKVESLKHVGGFTRIDKALNLASRSFY--LKSKNSKRLVVLLTNGLSMERVQTIQ-AAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDK---STDNTNEIW-DGIRSLM-LTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAF-TKRDIYVAIKQLRSNGNGGDLNTALNRARQVF-KIDYRADVAKIVVLFTDKLPTNQKDSLS-EAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRI--GIFTFNSEVYLNVPIDSNFENADM-QKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKA-KLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAIL--KFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD-TRKNATKTVIIIIDGEITDR-NETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAY--ISSELCDVIKGTNKECSTKQLDLMFALDSSSS 3863
N +++ ++ + +G + AL + + F R A+K ++++ D S A L GI VI + +G +L + + ++ ++ +K D D + KRI ++ A I+ L+ + K I+++ +G K R+S+V++ AV + F ++ DD+ +E++ V G + AL AS F K N+K+++V++++ S I+ +A L++S + + ++ +G+ A EL+ T P + + + T A K + N D+ F + S D+T + D +++L+ + + L+ + + D TT F T D+ I+ + DL L A +F + RA+ K++V+ +D++ N D +S A +L G +++I V + + E L + + + K +I A K+ + K+D+ I + + + + L F+ +IV ++ +R ++I G+ T+ + + +N+DM Q+ ++ +P G + + A+ + +F R D KVLV++TD + + A + + ++II+V VG ++ +L + G T + A+ I +A + P + D+ F + +++ FK M D + ++ DK VA+ + + D + S+E + K L +FK R++A K +++I D + R + E + L G+ + +G+ G + D +P K N ++ R + ++S + D IK + ++DL+FA+ ++++
Sbjct: 2641 NPAALEQKLNSVSRPQGIPDLKVALGEASKAF-RDSRPNARKILVVIVDNKSGSIVSDVRQAARSLDANGIEVIPVAIGPYANPSELEQTTPLKSNL-LQVSAKTKPGQLADTVMDKIE----KRINPERIPEADIIFALGAYNLNNYPPMKDVIKSMIDKYSMG--KIRYSVVTYGASAVEKLAFRDVFDSDDDLKLFIETILPVRGGPNLAAALEEASMIFKKDTKRPNAKKILVIISDKKSSNSPAEIERSAIQLEKSKIKVISLAIGNDASVAELE--KTTPE---TGNVVKASNGEMPEDLRDRITAVAFKDKLFIPNIDLGFALTADSLSADSTFRLMKDTVKALLEMYGRKRLHYAIVV-------FGDTPTTRINFGSVFPTDADLKAMIELIPRPRGRPDLVDGLKEATGLFLRQGTRANARKVLVVMSDRMSMNSVDDVSRRARVLHGRDIRVIPVAVGNQAYPTELEKL--TRMKDNLIKAKTSEDPSRLGKAIIEKAIKEY---VPVPKMDIAFAISANAT----SSEDRLGFIRQIVSSIIMRYGTSKINYGLVTYGDSPTTRINFGDSEQNSDMLQRVVDTLPHSSGGSNLEKAL-STVGLMFESARADARKVLVIITDRPSTSQADAEAKAAETLRNKNVEIIAVAVGAEVQPGKLRPLLPHGRNLINATTDERPDKKAEEIIKQAAKDPLEDNKV----DVGFAISATSKNADSTFKLMKDALITIFDKYHPDKIRYSVAVFGSRVQKITSFDDALPS-VDELKRSVEQAARLDGDPALDKVLKDFLEIFKGPRARRDARKVLVLITDKDSGLRKKDIIENSKPLNDLGVVIIPVGV-GDEVKKDFLETTTPLKK----NVIHIPRTESKEDLASRITDKIKNGLYQEHVPEVDLVFAMSAATA 3520 HSP 8 Score: 88.9669 bits (219), Expect = 1.726e-17 Identity = 195/878 (22.21%), Postives = 350/878 (39.86%), Query Frame = 3
Query: 1665 LGINKTRFSLVSFANEAVVEEEFTH----IYDDILKK-VESLKHVGGFTRIDKALNLASRSF---YLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP-------NPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQL-----RSNGNGGDLNTALNRARQVFKID-YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNS-----PYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFEN-ADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS-SGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLT--------KLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT-RKNATKTVIIIIDGEITDRNETFEEANL-LKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGT--NKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFG--LDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-NSTRKEVPKVIVLVTDGVS 4163
G + +++++F + V +F DD LK+ V S+ G + +A++ A R F + + K LVV++ S Q + L+E GV + + +GD+ EL L+ +P PF + + I+P T D+ F ++ E ++ ++ ++ + + + + Y + D+ + A+K + RS G G ++ A+ +Q+F D R++ K++V+ TD T ++ A L+ DG+K+I + L N+ P Y A+ K I D K +QD K LD+ I +++ G Q+ LD + +V+ + R I TF V +F N A +++++N + +G + A+G A+ F R + K+LVV+ D + I + +A GI++I V +G + EL V T + A T+ ++ + I +A D+IF +G+ + M D +KS+ + +VV + V L F + DS +D K F IE + G + AL A ++FK DT R NA K ++II D + ++ E + + L+K IKV ++ + S ++L+ +P+ V + N + +L D I + +DL FAL + S ++ D V+ ++ + V R FG + AMI + R G D+ + L+E L TR KV+V+++D +S
Sbjct: 2243 FGSKRINYAIITFNRRSQVALDFDQNKGLRNDDALKRLVASVSQEDGQPVLSQAMDEADRIFDGPTARPEAHKVLVVMIDKKSSSSLEDVKQKGHALEEKGVKVIPVAIGDEVHPGELAALTPYPEDIVTVAKPFYPPRLVDTL----------IRKAQQIRPSTPQI--DLAFALNSDGPRAVETFEYMKDVIKSVINKYGAYKLRYALIT--YGNSAAERISFDDQISN---TTALKHMLNQTERSRG-GSNIGVAIAALKQLFASDAVRSNARKVLVIMTDSQATGDLNAAKAAAKELENDGIKVITLALGPNADLDVAPKKKYSVTATTKEEPGSVANT------------IMD--KAKQDPMKDKVLDLGFAISATSADGASLHQVMLDAIKTMVRAYG--TENARYSIMTFGERPSEAVRFRESFPNPAALEQKLNSVSRPQGIPDLKVALGEASKA-FRDSRPNARKILVVIVDNKSGSIVSDVRQAARSLDANGIEVIPVAIGPYANPSELEQTTPLKSNLLQVSAKTKPGQL-ADTVMDKIEKRINPERIPEA--DIIFALGAY---NLNNYPPMKDVIKSMIDKYSMGKIRYSVVTYGASAVEKLAFR--DVFDS--DDDLKLF---IETILPVRGGPNLAAALEEASMIFKKDTKRPNAKKILVIISDKKSSNSPAEIERSAIQLEKSKIKVISLAIGNDASVAELEKT-TPETGNVV------KASNGEMPEDLRDRITAVAFKDKLFIPNIDLGFALTADSLSADSTFRLMKDTVKALLEMYGRKRLHYAIVVFGDTPTTRINFGSVFPTDADLKAMIELIPR---PRGRPDLVDGLKEATGLFLRQGTRANARKVLVVMSDRMS 3062 HSP 9 Score: 86.2705 bits (212), Expect = 1.125e-16 Identity = 144/629 (22.89%), Postives = 271/629 (43.08%), Query Frame = 3
Query: 2343 RSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDS-LSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFEN-ADMQKEINKIPFIEGNTAIGWAIGNA-TDYLFTKGREDVPKVLVVLTDGA-----NDIGESPQ-ISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLT--------KLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEIT-DRNETFEEANLLKKDGIKVFTIGL-TGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKV--GVVTFNTDARKEFGLDQFTEKYAMISAVKRII----FDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTD 4154
+++G +L AL ++ +F+ + R K++V+ D N + L+ A L+G+G+++I V L + + +NL + +N + + EQ+ N I R + M +D+ I S+ + F++ + V +++ + + IF + +LN F + ++++ I +P G + A+ A T + R +V K+LVV+ D ND+ E+ + + +K I++I V +G KE+ + ++ +N+ T EK K + + Q K + DL FV+ ++A +S F++M D +KS + +++ NT V L F+RG + K D T+G + KAL LF+ R N TK ++I+D T D E + A L+ GI V + L G E L A D + V++ ++ + + ++ N+ ++DL FA+ +S+S P+ + + +I+ N TK+ ++ + + R F + S +KR + + + AL K LNS R + KV+V++TD
Sbjct: 7090 KASGASPNLQLALQTSQSLFE-EARPSARKVLVVVMDSKSGNSPNEILTAAASLEGNGIRVIPVALGDATDHAELDNLTPITDDVVTDNKDWPKETAEQLMNRI------RDENAYMPAIDLGFAISASSLDKAKTFELMKEVVGQVIAKYGTTRLSYSLIIFGSSPTTFLN--FGQKFPDLGNLRRLIESLPPSFGTPNLKEALKEAKTIFNNAPKRSNVKKILVVVMDSESSSEINDVSEAAKSLEQLK-----IRVIPVLIGPDTDNKEMESLTTNK--HNLIRATGDEK--PKDLRDVIMQTVLKDIVPVPEMDLSFVLSATAVDSSTTFRKMRDVIKSAVDSLGMGRVRYSIITFGNTPVVSLPFSRGFPTEDALKR--------YVDSIPRTDGAVLDKALDKTIELFRQQGRPNVTKIAVVIMDKTSTSDIEEIRKRAKPLEDRGIIVIPVSLGPGANIEELLSITADHDNLIGVNDSTDPEVVMRVVMDKIA------NQRPRVPKIDLGFAISASASGAPENFAKMKSVIAKIID----NYGTTKIRYSLLIYGSTPRVHI---PFQKTPPTASDLKRYLQGAPLNTPRASLAMALETGKQMFLNS-RNDASKVLVVITD 7678 HSP 10 Score: 82.0333 bits (201), Expect = 2.259e-15 Identity = 222/1111 (19.98%), Postives = 452/1111 (40.68%), Query Frame = 3
Query: 948 SNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSL--QIDIKFSQVGVLIYSDILEMSLPLGSK-QNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQ--VQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSMERVQTI-QAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDK-STDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAF-TKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID-YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFE-NADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-REDVPKVLVVLTD-----GANDIGESPQISAVKAKLGG--IKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTK-----------LTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNET-FEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKE-CSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLN-----STRKEVPKVIVLVTD 4154
+ I STP Q+DL F L++ A+ + + +VIKS+ + + ++ Y + + + N T ++ +++ E G + I A+ + F S R A+K ++++TD + A++L+ GI+VI + LG + D K + T E+ +A I A Q P ++ LD+ + +S LH+ + L K + + G R+S+++F F + + + +K+ S+ G + AL AS++F N+++++V++ + S V + QAA L +G+++ + +G A+ EL+ + + L+ + + + V K I P+ DIIF + + +N + D I+S++ S R S++ Y + + F + D+ + I+ + G +L AL A +FK D R + KI+V+ +DK +N + + I L+ +K+I + + ++ E + ++ + ++ ++ + AFK D+ + +D+ + + F++ D V +++ + R I F + S F +AD++ I IP G + + AT +G R + KVLVV++D +D+ ++ L G I++I V VG Q EL + + KT D + I + P + D+ F + ++A + +R + + S+ +T D TR+ N+ DS D + ++ + S+ G + KAL++ ++F+S R +A K ++II D T + + + A L+ +++ + + + L+ + + L N + +R + + ++IK K+ ++D+ FA+ ++S ++ D + I + + + V F + +K + F + + +KR + D AL + + L R++ KV+VL+TD
Sbjct: 2390 AQQIRPSTP-------QIDLAFALNSDGPRAV---ETFEYMKDVIKSVINKYGAYKLRYALITYGNSAAERISFDDQISNTTALKHMLNQTERSRGGSNIGVAIAALKQLFASDAVRSNARKVLVIMTDSQATGDLNAAKAAAKELENDGIKVITLALGPNADLDVAPKKKYSVTATTKEEPGSVA----NTIMDKAKQDPMKDKV----LDLGFAISATSADGASLHQVM--LDAIKTMVRAY--GTENARYSIMTFGERPSEAVRFRESFPNPAALEQKLNSVSRPQGIPDLKVALGEASKAFRDSRPNARKILVVIVDNKSGSIVSDVRQAARSLDANGIEVIPVAIGPYANPSELEQTTPLKSNLLQ--VSAKTKPGQLADTVMDKIEKRINPERI-PEADIIFALGAYNLNNYPPMKDVIKSMI------DKYSMGKIRYSVVTYGASAVEKLAFRDVFDSDDDLKLFIETILPVRGGPNLAAALEEASMIFKKDTKRPNAKKILVIISDKKSSNSPAEIERSAIQLEKSKIKVISLAIGNDASVAELEKTTPETGNVVKASNGEMPEDLRDRITAV--AFK---DKLFIPNIDLGFALTADSLSADSTFRLMKDTVKALLEMYG--RKRLHYAIVVFGDTPTTRINFGSVFPTDADLKAMIELIPRPRGRPDLVDGLKEATGLFLRQGTRANARKVLVVMSDRMSMNSVDDVSRRARV------LHGRDIRVIPVAVGNQAYPTELEKLTRMKDNLIKAKTSEDPSRLGKAIIEKAIKEYVPVPKM-----DIAFAISANATSSEDRLGFIRQIVSSIIMRYGTSKINYGLVTYGDSPTTRI--------NFGDSEQNSDM--LQRVVDTLPHSSGGSNLEKALSTVGLMFES-ARADARKVLVIITDRPSTSQADAEAKAAETLRNKNVEIIAVAVGAEVQPGKLRPLLPHGRNLI--NATTDERPD----KKAEEIIKQAAKDPLEDNKVDVGFAISATSKNADSTFKLMKDALITIFDKY--HPDKIRYSVAVFGSRVQK---ITSFDDALPSVDELKRSVEQAARLDGDPALDKVLKDFLEIFKGPRARRDARKVLVLITD 3429 HSP 11 Score: 82.0333 bits (201), Expect = 2.259e-15 Identity = 230/1072 (21.46%), Postives = 438/1072 (40.86%), Query Frame = 3
Query: 1092 QIDIKFSQV--GVLIYSDILEMSLPLGSKQ-NKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIE--------QFELGINKTRFSLVSFANEAVVEEEFTHIYD-----DILKKVESLKHVGGFTRIDKALNLASRSFYLKS--KNSKRLVVLLTNGLSMERVQTIQA-AYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVA-IKQLRSNGNGGDLNTALNRARQVFKI--DYRADVAKIVVLFTDKLP-TNQKDSLSEAEILKGDGVKIIFVGL-SKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATD-----YLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS-SGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDY---KNFESSIEDMT-TSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEIT-DRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNF--SNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDF--VERIVRDM--DINANFTKVGVVTFNTDARKEFGLDQFTEKYAMI-SAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSP 4181
QI K +Q+ V+++ + L G + + ++ ++ + +G + + + F + +GA+K +++V D + + A+ L+ GI+VI + LG D+ M+ + Y + +T + Q QI + AK D+ + + L N L K FI + G + + LV F VV T Y ++L + + VG + + AL A +L+S +++++V+ + S + T++A A L G+ + + G+ + ++EL+ ++ + K K + + ++ K+ +KP D+ FV++ + N+ ++ ++ + ++ + + D + E F D+ I + + G L+ AL AR+VF R K++V+ TDK + D + L+ DGV++I V L S+ P + + +K+ I I + + K E+ +I +AF D KLD++ + S+ + FQ D + +V+ + R G+ F S + D F N ++ + K NTA I + + R KVLVV+ D +D + A K K I VG + EL I + G T E K I+ P + DL FV+ +++ + + +M D +KS + +N R +++ F + F + + S I+ + TS +G + KAL AR LF S++R +A K +++++DG+ T D E ++A L+K G+ V + S+ ++ ++ +P + DN +N ++ ++ D ++ ++DL FAL SSS Q+ +F ++ +V +M + T+ G++TF + +++ +VK I GA+ + + L R KV+V+V D S G SP
Sbjct: 6121 QIVKKHNQLRYSVILFGETPSTKLTFGQRMYDANTLKGLMTTWAMPKGDPDVVEVMAEANRLFTNADVQGARKILVVVMDKSSKNSMADIRGAAKDLEAAGIKVIPLLLGRFKDTDEQEAMTPNKGYI-------VTANNTDNPKQIGNQI-----VNKAKKDLPSVPELDMVFALS--ANSLNADKNFIRFKDIVQAVVHQYGSGRVHYGLVVFGQLPVVYARLTENYSGQQLRELLSTIP--RSVG--SSLSSALYKAEE-LFLESIRPEARKVLVVFIDRSSSSNIDTVKARARSLWSRGIRVIPVLFGESS-KDELESITGDKRNIVTD--KGNKPPKDIADNIAKKSVAEMKP---VPEVDMAFVINARSTNSASNFEKMKDTVKAIIEEYGSRQIKYSLI--QVGDTPSVELNFRNTFPGDDLLKRYISDILQDSGGSALDLALEEARKVFDPAQGARPKAKKVLVIITDKKSDSTVSDIKAVTRSLQDDGVRVIIVALGSEVQPSALSKIIPNKQDIINITDTAQPKKIAEK---IIKNAF---NDSINAPKLDLLFAVSSSSPDADQTFQHMQDTLQSVVETYGMHS--IRYGLVVFGSAPDVKFTFDEYFPNTEV---LRKRMLTANNTAGKPDIVKVLEKAVLMFASASSRPMAKKVLVVMIDAKSDNSADELLEAAKPLKN--KKIKAPVGSKAVPDELTTITTDKGNVIVTNTSQPSETLVNKLINKARTDKRDVPVL-----DLGFVISATSRDAKDVYAKMKDVIKSF--INRYGTKNIRYSLITFGDDPRVKLSFSSNLGSDRELRSFIDVIPRTSDSGRSLDKALEQARQLFTSESRPDAKKVLVVMLDGKSTSDPRELPKKATALEKAGVTVIPLVFEPSGSKDEM-ALITP----YKDNIISANKSSDPMDVAEQILDKSLALERKPRVFEVDLAFALGSSS------LQSSANFEKMKELVTEMIEKYGSYKTRYGLLTFGDRPSIRMKFNNNPPSNSLLKQSVKFIPKASGASPNLQLALQTSQSLFEEARPSARKVLVVVMD--SKSGNSP 7132 HSP 12 Score: 81.6481 bits (200), Expect = 2.499e-15 Identity = 241/1146 (21.03%), Postives = 459/1146 (40.05%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDI--KFSQ----VGVLIYSDILEMSLPLGSK-QNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDI--LKK-VESLKHVGGFTRIDKALNLASRSFYLKS--KNSKRLVVLLTNGLSMERVQT-IQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLR----QDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDE-TTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKID-YRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQK-EINKIPFIEGNTAIGWAIGNATDYLFTKG-REDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY-NVKTFTDLEK---------------------------------------FNA-KTISNEA-CQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKS-LTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNAT--KTVIIIIDGEI-TDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVI--KGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDM--DINANFTKVGVVTFNTDARKEFGLDQFTEKYAMIS--AVKRIIF----DDGATDIGEALRECKNELLNST-RKEVPKVIVLVTDGVSTGGL 4175
+LDL+F + ASS +E + ++K+ I I ++ + GV+ YS+ G N +++ + L + I+ AL S + + +G R A K ++L+TD A + ++ L +++I + +G L S +S I Q + + A++D+A + +S Q + + + K IE++ GI++ SL+ + + F D LK VE+L V G + + KAL A++ F N+K+++V++T+ S V + ++AA L+ +G+ + + +G AD EL+ + ++ +D L ++ + P + D+ F V + N++ ++ ++ + + R + Y D+ TTT F D + I L+ +G L AL +++F+ R V K +V+ TD+ T A+ L GV ++ + + + A+ S + + I + +DE +D+ I S+S E F++ + + I+ +D R + F S + + + ++ K +I+ + I G+ + A+ +A ++G R K LV++ D A G + +A + GI++I V VG++++ D+ S+ F N+ + D E NA KT + A +P+ P + L+F + + A ++ E F M + + ++K T+ + ++ F + K Y + +M + I K LTSA LFKS T + K ++I+ID + T + + A L++D +KV + + + S+L +V SP+K S ++ +L D I K NK LDL+FA+ S+ G D LD +++ + + ++TFN R + LD F + + + A+KR++ +DG + +A+ E T R E KV+V++ D S+ L
Sbjct: 1248 ELDLVFAISASS-------REASIAYKLMKATLISILDQYGRGDFRYGVITYSNYATRRKGFGDNYPNIEDVKTLIRYLPPNTRGSAINRAL--SAADAMFRGPFVRPTADKILVLITDNASGMTVEEIRAASKPLTDNKVKIIGVAMGNQASVPDLQAASGNPDNVMQAKLSSKPNKLGNKIMQKVLE----REPSIAEIDLAFALSATSAPQHFQVMKDTV--KSIIEKY--GIDRIHLSLIVYGDLPYTRVSFGQDIPDARTLKNLVEALSRVSGGSDLGKALAEATKVFRNDDVRPNAKKVLVVITDSKSSYGVASLVKAAEPLEHAGIRVLAVAIGADADASELRSIVQHQGDVIKASSSEDPSGLADRLVSAMLKETRKIPKV---------DLGFAVSAGSSNSDATLRHMKDIIKSIVTRYGAERMRYGLMS--YGDDVTTTIFFTDGITNPNSLLPFIDILQKTPSGSSLEKALVGGKKLFEHGGTRPGVDKFLVVITDRKSTGDAAQTENAKADLAKAGVNVLVIAVGSEVDHKELNETATTPSHKLVTAPGESPAEVADRVVDIIN-----KDELDFTAVDLGFAI--SSSDVKETFKLMKESIKSIINTYG--RDNIRYAVMVFGSSTRVVLRFGEALPSLEILKMKIDGVGPIPGDPNLKTALEDAKRMFESRGARPKAKKFLVLMMDRRAQQPGSAVNRAAGDLERSGIRVIPVSVGKRVAPV---DMESTTPFRDNIISVPDSESPAGLGKKITDKIKQTYVILLMRSIHGKKMSTTISYVIVSLNAFKTFTLRADIGVPKIPDV-----QLVFALSAVAAESEENFNHMKSVVNAVISKYTL---HWIKFGLITFGSKVTVWENMKGIYPTEAVLRKFVNNMPKPSGRPAIDKVLTSAAKLFKSQTPSEPSTKKALVILIDKKSGTTPADIIKAATPLEQDKVKVVAVAIGNEADVSELGNV-SPEKG------STIKGDKGVNPDDLADKIMNKVFNKYAGVSPLDLVFAI---SAAGVD---DTLDQMKKTIESTIDTFGSKRINYAIITFNR--RSQVALD-FDQNKGLRNDDALKRLVASVSQEDGQPVLSQAMDEADRIFDGPTARPEAHKVLVVMIDKKSSSSL 2329 HSP 13 Score: 78.1814 bits (191), Expect = 2.627e-14 Identity = 193/908 (21.26%), Postives = 363/908 (39.98%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSL--QIDIKFSQVGVLIYSDILEMSLPLGSKQNK-TEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYY-------PIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEA--VVEE---------------EFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYD-LQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIF-------TFNSEVYLNVPIDSNFENAD-MQKEINKIPFIEGNTAIGWAIGNATDYL---FTKGREDVPKVLVVLTDGANDIGESP-QISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYI----DSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIID 3584
D++F L A+ DQ +K L + I + GV+++ + + ++ G + N +++ K +S LE +G + AL R+ +F + R+GAKK +I D + ++++L MG+++I + +G S I Q K S +T P + ++ + ++ Q PKP LDI + SS + K ++ + G +++V+F A VV+ E + D I K E V RI ++ A+ S N+K+ ++++T+ + V + A + +++ G+ + +G+ D D EELKI++ N + D + + E+ + T K DI + + +++ + IR ML L +N + + + F + +I + LR D+ L AR+ + + RA K++V+ T++ +N L A I L ++II V L ++ N LA K + EQI + +A R+D + KL + + I ENFQ + V IV +R G F S+ + + F + D + + I+ + + G+++ L F ++ KV VV+ D + S + +A+ ++ GIK+I V + EL + + + + A+ I +A ++ P I + DL+F + S AN + F +M D +K + +E ++ F + D+ +D + I + + +G + KAL A +F S R A+KT+++I D
Sbjct: 4621 DIVFALSATGQ---QRDQSFRMLQEAVKGLVARYGIGSVRYGVIVFGNTVSTAVDFGDQFNSASDLMKAISNLERKDGGPRLGLALDRAKRQFSNTNRKGAKKILIFTLDQKPTTDEASIRSKSDELNSMGVKIIPLLVG-SEIGPQDVKPVSGDTKNIEVSVTDPPDHVTDKLYKQIGEAVKEESQ-PKP------SLDIGFAISASSADSDANFAKMKNIIKAVLDIY--GTEGVHYTIVTFGQTAKTVVQNSKGAELRAGLPTLATELKRLIDRITKNPEGTSIVSALNRIKQSFKEATSS----RPNTKKTLIIMTDSNHEDGVDDVIIAMEGVEDDGIQVLYVGVDDSVDLEELKIVAPDDNHVISADGRTPGDVAEQIARRINATRNIPK-------SDIALCISPPSSGSDKDYSRIRD-MLKNL--MNQKGSENLRYTGVIMEANPKVFTMRRGPGGWNIDELVASLRRASGRPDIVKTLTMARRSLE-NGRAGAKKVMVILTNQQSSNLPSELKAAAIPLDRAEIRIITVPLGPDANTN---ELAKITPVKKDLLRVSPGASTEQIATAVRNAL--RRDRLRVPKLQLAIAISAGAIKAVENFQKMKEVVKSIV---------SRYGSFNISYSVVAMGSDPAVQIAFSKTFPDDDALMRAIDGVQW-----TFGYSLAKGLRELKSVFASEGQETRKVAVVIMDRRSSSSVSEVKRAALDLEVAGIKVIPVAFEGYSDKSELGKTTPNKQNLIARMPLESPDTTAEKIVRKAVKV--LPPISEL--DLVFAISSVANPVAN-FPKMLDIIKQ--SVQQYGQERIHFYVIVFGSRAEVKLRFDASLSDD--QVKKKISGIRPNDDGDSLDKALELASDVFGAASAKRPGASKTLVLITD 5472 HSP 14 Score: 75.485 bits (184), Expect = 1.769e-13 Identity = 131/613 (21.37%), Postives = 250/613 (40.78%), Query Frame = 3
Query: 2367 LNTALNRARQVFKID-YRADVAKIVVLFTDKLP--TNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXX--XXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENAD-MQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTD--GANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASS-GEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGE-ITDRN--ETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELC-DVIKGTNKECS---TKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGL-DQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTD 4154
L AL AR +FK+D R ++V+ TD T+Q D S A L + +++I VGL E++ K+ + + + KE + ++ +RQ +D+ + I G F + +++ L R G+ F + F + D ++ +P G +A+G A+ NA T+ R + KVL++ D ++D+G + S KL G+++I V + E+ +A G + K ++ + + P+I D++ + ++ + FK M + ++S+ + K + V + I + + +S I G I KAL AR ++ D R +A + V+II+D + + D + A ++KDG+ V +GL E++L + +P K +++ R +L D++ + S K +D++FA+ SSS P + ++R++ ++ K+ + F D+F + S + I G ++ EAL + +NE R K++V+V D
Sbjct: 3958 LGAALREARDMFKMDGVRPHARHVLVVITDSSSGLTDQ-DISSAARPLHLNEIRLIPVGLGGLIGKKELESMTPVKADVIHVAKDTEPRYLGKEIMSRVVQALIPKRQ-------MDIAIAIGSGGPNGDATFTQAKEITKKLLARNPL--GAARYGLIVFGDAAFTETTFSREFPSQDDFLALVDAMPRRSGGSALGNALENARTMFHTEARPNAEKVLIIFIDQRPSDDVGVAINSSIALDKL-GVRVIVVAMQMPNVPVEIEQVAPDRGSIIRANNNDKPQAIADKILNLSKKDVKRIPSI-----DMVIAISATVARPDRTFKSMKNTIRSIIDQYGIAKIHYSVIVFGDRPVTKITFQDRLSPSDLKSYIRTFARVNGGPSISKALQQARYEWEQDARPDAHRVVVIIVDNKSVGDSGNIKKLRAAKEMEKDGVIVIPVGLGRDFDENELHLI-TPYKE------NSIARVTLPDPRQLARDIMDKSLMAISKPLVKPMDVLFAVSSSS---PQNLLKAKELIKRMIDTYGMDK--LKISYIGFGDKTTSPISFKDKFPSAEDLKSRIDVISLVPGRPNVLEALDKARNEFETGGRSNAKKILVIVVD 4542 HSP 15 Score: 65.0846 bits (157), Expect = 2.619e-10 Identity = 108/480 (22.50%), Postives = 202/480 (42.08%), Query Frame = 3
Query: 1191 QKFVSKLELLEGATFISNALRRSGEEFISK--------------GREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSST--ETYYPIEDMSKMALFDTT--DIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF-THI--YDDILKKVESLKHVGGFTRIDKALNLASRSFYLKS--KNSKRLVVLLTNGLSM----ERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKI--DYRADVAKIVVLFT-DKLPTNQKDSLSEAEILKGDGVKIIFVGLS 2540
Q+F S ++ F+ +A + SG+ ++K GR AKK +IL+TD NS K + L+K +RVI + L SV L K++ T + P+ + D I QD IP ++D+A + + + K I ++ G K R+S+VSF ++ F H+ D +LK +ES G + KAL + F + ++KR +V++ +G S E ++++ L V + T+ + A+ +EL +++ + ++ K + L V I P D++F + D ++ I+++ + + + R + + Y T R + +T + I+ L+ + D+ AL A +F R + K++V+ T D+ +++D LS A+ L + +++I V LS
Sbjct: 8209 QRFASDEQM---KLFVDSAWQPSGKPDLAKALKEVNALYGIAAGGRPDAKKVLILITDNDANSSDEKTKAALRSLEKSSVRVIAVPLYHSVDTTSLEKITVTKDDVIKPVPSAMPDTVVDKIIGRILQDTKIIP--------EVDLAFAISSTMLDSGESFFTMRNIIKHIISKY--GRVKIRYSVVSFGSDPTTILRFDQHLSNSDAVLKSIESSPRPTGKPDLVKALQEVRKQFEAAAPRPDAKRFLVVIMDGRSKNTKEELMKSVVPLERLPSGHVKVITVPIPPDANEQELSAVASNKDNVIKAMIKPDPQGLGDRVIDKIIEDNPIVPTM-----DVLFAIGAKPDTPTSTFNNIKAIAKSVVSTYGKDRIGYSLLV--YGTTPTVHIRFRDGYTVEQLGDFIELLQRPNDSPDVAAALRVAADIFDPAQGSRPEAKKVLVVITADESGRSRQDILSSAQDLFRNWIRVIVVALS 8668 HSP 16 Score: 65.0846 bits (157), Expect = 3.177e-10 Identity = 114/537 (21.23%), Postives = 228/537 (42.46%), Query Frame = 3
Query: 2664 KQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLG-----GIKIISVGVGQQISEKELNDIASSGEFYNV------KTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKN---FESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIIDGE----ITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCD-VIKGT-NKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDD---GATDIGEALRECKNELLN-STRKEVPKVIVLVTDGVSTGGLSPELEST 4196
K ++D ++ +LD++ + ++ +NF D V +V R G+ F + + N+ +++ ++ IP G +++ A+ A + R + KVLVV D ++ S I VKA+ GI++I V G+ S+ EL I +G+ N+ K D+ AK E +P+ D+ FV+ + + ++ F++M D +K++ + + ++++ ++ F+ + + I D+ + G + AL AR +F R A K ++II D + ++D L+ DG++V + L + S L + P+K ++ Q ++ + +IK N + +LDL+FA+ SSS +Q D ++ +V +++ + G+V F + +F D++ ++ KR++ + G DI + L + + S+R KV+V++ D S LE+
Sbjct: 6267 KAKKDLPSVPELDMVFALSANSLNADKNFIRFKDIVQAVVHQYG--SGRVHYGLVVFGQLPVVYARLTENYSGQQLRELLSTIPRSVG-SSLSSALYKAEELFLESIRPEARKVLVVFIDRSS----SSNIDTVKARARSLWSRGIRVIPVLFGES-SKDELESI--TGDKRNIVTDKGNKPPKDIADNIAKKSVAEMKPVPEV--------DMAFVINARSTNSASNFEKMKDTVKAI--IEEYGSRQIKYSLIQVGDTPSVELNFRNTFPGDDLLKRYISDILQDSGGSALDLALEEARKVFDPAQGARPKAKKVLVIITDKKSDSTVSDIKAVTRS---LQDDGVRVIIVALGSEVQPSALSKII-PNKQDIINITDTAQ------PKKIAEKIIKNAFNDSINAPKLDLLFAVSSSSPDADQTFQHMQDTLQSVVETYGMHS--IRYGLVVFGSAPDVKFTFDEYFPNTEVLR--KRMLTANNTAGKPDIVKVLEKAVLMFASASSRPMAKKVLVVMIDAKSDNSADELLEAA 6769 HSP 17 Score: 59.6918 bits (143), Expect = 1.064e-8 Identity = 99/399 (24.81%), Postives = 174/399 (43.61%), Query Frame = 3
Query: 2712 TIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENAD-MQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTD--GANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDI-ASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENT---RVAILKFNRGNYIDSGFKE------DYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFE-EANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVI--KGTNKECSTKQLDLMFALDSSS 3860
+D ST+ F+ D + V +L + R R I TF + +++P F D +++ ++ IP +G + A+ + +GR +V K+ VV+ D +DI E + A + GI +I V +G + +EL I A V TD E + A Q P+ P I DL F + +SA+ E F +M + ++D T R ++L + + F++ D K + ++ +T + AL + + +F ++R +A+K +++I D + E A LL+ GI+V +GL + +L+ + S K ++F Q+ N L VI K N + ++DL FAL +SS
Sbjct: 7398 AVDSSTT-----FRKMRDVIKSAVDSLGM--GRVRYSIITFGNTPVVSLPFSRGFPTEDALKRYVDSIPRTDG-AVLDKALDKTIELFRQQGRPNVTKIAVVIMDKTSTSDI-EEIRKRAKPLEDRGIIVIPVSLGPGANIEELLSITADHDNLIGVNDSTDPEVVMRVVMDKIANQRPRVPKI-----DLGFAISASASGAPENFAKMKSVIAK-----IIDNYGTTKIRYSLLIYGSTPRVHIPFQKTPPTASDLKRY---LQGAPLNTPRASLAMALETGKQMF-LNSRNDASKVLVVITDSKSVSSKSEVEEAAALLEMAGIRVIPVGLG--FFDKELEMITSNKK----NSFQADQKVN---PETLGHVIMKKVINDTPNVPRMDLAFALGASS 7764 HSP 18 Score: 59.6918 bits (143), Expect = 1.073e-8 Identity = 109/506 (21.54%), Postives = 214/506 (42.29%), Query Frame = 3
Query: 2742 ENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFE-NADMQKEINKIPFIEGNTAIGWAIGNATDYLF---TKGREDVPKVLVVLTDGANDIGESPQ---ISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAG----DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYI------DSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT-RKNATKTVIIIIDGEITDRNETFEE-ANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVI--KGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDIN---ANFTKVGVVTFNTDARKEFGL-DQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-NSTRKEVPKVIVLVTDGVSTGGLS 4178
ENF VN ++ L + G+ TF S+V + + + A ++K +N +P G AI + +A LF T K LV+L D + G +P +A + +K+++V +G + EL +++ T + N ++++ K AG DL+F + SA + QM ++S + + AI+ FNR + + + G + D + + ++ + +A+ A +F T R A K ++++ID + + E ++ + L++ G+KV + + + +L ++ P+ + V + L D + K ST Q+DL FAL+S GP +T ++++ +++ + IN A + ++T+ A + DQ + A+ + + G ++IG A+ K ++ R KV+V++TD +TG L+
Sbjct: 2042 ENFNHMKSVVNAVISKYTLH--WIKFGLITFGSKVTVWENMKGIYPTEAVLRKFVNNMPKPSGRPAIDKVLTSAAK-LFKSQTPSEPSTKKALVILIDKKS--GTTPADIIKAATPLEQDKVKVVAVAIGNEADVSELGNVSPE----KGSTIKGDKGVNPDDLADKIMN----KVFNKYAGVSPLDLVFAI--SAAGVDDTLDQMKKTIES--TIDTFGSKRINYAIITFNRRSQVALDFDQNKGLRND-DALKRLVASVSQEDGQPVLSQAMDEADRIFDGPTARPEAHKVLVVMIDKKSSSSLEDVKQKGHALEEKGVKVIPVAIGDEVHPGELAALTPYPEDIVTV--------AKPFYPPRLVDTLIRKAQQIRPSTPQIDLAFALNSD---GPRAVET-FEYMKDVIKSV-INKYGAYKLRYALITYGNSAAERISFDDQISNTTALKHMLNQTERSRGGSNIGVAIAALKQLFASDAVRSNARKVLVIMTDSQATGDLN 2516 HSP 19 Score: 56.225 bits (134), Expect = 1.246e-7 Identity = 110/480 (22.92%), Postives = 211/480 (43.96%), Query Frame = 3
Query: 2814 RIGIFTFNSEVYLNVPIDSNFE-NADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLG--GIKIISVGVGQQISEKELNDIASSGEFYNV--KTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVM---GSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIE-------DMTTSTNGVFIGKALTSARVLF--KSDTRKNATKTVIIIIDGEITDRNETFEEANL--LKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFN--TDARKEFG---LDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLS 4178
R G+ F + + + + ++D+++ ++ I + +++ ++ A +GR D VLV++TDG ++ +I + L + II V G + +L +A+S + Y + KT D + + + + + + T DL FV+ G A +T + KQ+ + ++ ++ D T +++ + FK D K F S E ++ + + KAL SAR LF ++ +R+ A + +++I D E +D + +A + L +D I++ ++ L G+ +E++LKSVA PD + V N S +++K E K ++++FA+ SS G Q +D V +IV+ N + V+ F + FG D T K M + G D+ E + E N+ + K++V+V D S ++
Sbjct: 5764 RYGVIVFGGVPVIKMGFEKEYRSDSDIKRYLDSINREKQGSSLHRSLEEARKVFVQQGRPDAQHVLVIMTDGQSE-SLPDEIETARNPLDQMNVTIIPVAFGDESDPAQLR-VATSDDRYLIVSKTTVDPSELGQQIMDRLSAESKVQET------DLAFVISAAGPDAQQTFSKIKQIIGLV--ISNYSIGD---TLYSLVTYGDRPVTHVQFK-DSKKFPSDSELRQFIRNSVSKPSGAADLYKALMSARDLFSKEAGSREKAARVMVVISDRE-SDSSLMDVKAAVSGLIQDNIRLISVALGGQGNENELKSVAPVPDAVISVG-------INDSSSKAGGEIVKQVQTEPVPK-MNIVFAI---SSRGKARSQA-IDVVTQIVK----KHNQLRYSVILFGETPSTKLTFGQRMYDANTLKGLMTTWA----MPKGDPDVVEVMAEANRLFTNADVQGARKILVVVMDKSSKNSMA 6208 HSP 20 Score: 56.225 bits (134), Expect = 1.366e-7 Identity = 160/818 (19.56%), Postives = 333/818 (40.71%), Query Frame = 3
Query: 1851 NSKRLVVLLTN---GLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFL----RQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSL--NXXXXXXXXXXXDYSDETTTEFRLDEAFTKRD-IYVAIKQLRSNGNGGDLNTALNRARQVFKID-YRADVAKIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGL-SKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPI-----DSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-REDVPKVLVVLTDGANDIGESPQISAVKA-KLGGIKIISVGVGQQISEKELNDIASSGEFYNVKT---FTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRV------AILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSD-TRKNATKTVIIIIDGEITDRNETFEEA--NLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFG----------------LDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNEL--LNSTRKEVPKVIVLVTD 4154
N++++VVL+++ G S +Q I L+ VD+ + +G++ +EL +L+ N + ++D + + + P I D++F + S + D +LM T Q + + R S++ + +T+T+ D RD + I + ++ A A ++F++ R + K+VV+ D+ D L + L V +I V + S++ P + + IK + QI I K + +LD+ V + + + F + + + I+ DR +G + VY ++ + ++ ++++ I ++G +A+ A+ ++ L G RE+ KVLV++TD + + ++ +A K + I ++ + VG ++ +EL E NV T + E AK I I + P + D++F M +++ ++ + F M + +KS+ + + V A +K + G + S +ED + SI+ M + KAL A+ +F+ + R NA+K +++++D + + + +A LLK G+++ + + L+ V + ++ +S + D I K+ +++LMF + ++S + D ++R++ D + V+ + + E L+Q A+ S + + D + I L E + + R E KV+V++TD
Sbjct: 403 NTRKVVVLMSDKGSGWSKSFIQGI--IQPLETRYVDVIPVAVGNEGSEDELLLLTPNKNDLVTVSKKEDPIRLGDKIMGKIYPEIEAIPEI---------DLVFAISASASDP----DTTLALMKKTAQHIVDTYGTSKIRYSLVIFGSQTSTQLSFDRKMPDRDSLSSMIAGMPRIPGRPAIDQAFLEADKLFQLQGTRPNARKVVVMMVDQRSAATDDDLRRSSRRLHQRKVDVIPVAIGSESDPDQLLHTTSDNTNLIKAAKSELPNSLANQIMQKIVQGRK-------VPELDLAVAVSATATDADTTFMLAKNALKSIM-------DRYGVGAIRLSFIVYSDMALKKFTFQDKYDEEELKRSIESAMRMDGGSALDKALEAGSEVLQGPGARENAHKVLVLITDMTSGLSDAEIEAAAKPLQQKQIAVVPIAVGNDVTTRELEKTTPYRE--NVMTAYRAENPEVLGAKIIDKILQAIRRVPKM-----DIVFAMSATSAQSDQMFSLMKNSIKSIIDMHGTTDIHYSVILYGDTATIKRDFGASVPS--EED---LQRSIDAMQPLLGRALVDKALQLAQQVFEGEGVRPNASKILVVMMDMS-SPLSVAYLKAKTRLLKDSGVRIIAVAAGQAVDANKLEQVLPKGGVISAPEGTS----PGNLSKSIMDTILKAPKKP---EINLMFMVSATSQRASQIYTLMKDTMKRVI-DKYGTRDQVHYSVIVYGSSPTTEITFQDSYNIAEAHIITVVLEQSVSPVALKSDIDALPRDSSGSAITRTLDEALKQFATYDGVRPEASKVLVIITD 1170 HSP 21 Score: 56.225 bits (134), Expect = 1.437e-7 Identity = 87/352 (24.72%), Postives = 161/352 (45.74%), Query Frame = 3
Query: 999 LDLIFVLDASSSLA--LYWDQELAFTNNVIKSL--QIDIKFSQVGVLIYSD----ILEMSLPLGSKQNKTEIQKFVSKLELL-EGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQD----ACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTH--IYDDILKKVESLKHVGGFTRIDKALNLA---SRSFYLKSKNSKR--LVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREEL 1994
LDL FV+ A+S A +Y A +VIKS + K + ++ + D L S LGS + E++ F+ + + + AL ++ + F S+ R AKK ++++ DG S + +A L+K G+ VI + S KD++ + T Y +S D D+A+ + + + R+ ++D+A + SS + IE++ G KTR+ L++F + + +F + + +LK +S+K + + L LA S+S + +++ S R LVV++ + + + AA L+ +G+ + + LGD D EL
Sbjct: 6830 LDLGFVISATSRDAKDVY-----AKMKDVIKSFINRYGTKNIRYSLITFGDDPRVKLSFSSNLGSDR---ELRSFIDVIPRTSDSGRSLDKALEQARQLFTSESRPDAKKVLVVMLDGKSTSDPRELPKKATALEKAGVTVIPLVFEPSGSKDEMALI----TPYKDNIISANKSSDPMDVAEQILDKSLALERKPRVF--EVDLAFALGSSSLQSSANFEKMKELVTEMIEKY--GSYKTRYGLLTFGDRPSIRMKFNNNPPSNSLLK--QSVKFIPKASGASPNLQLALQTSQSLFEEARPSARKVLVVVMDSKSGNSPNEILTAAASLEGNGIRVIPVALGDATDHAEL 7163 HSP 22 Score: 55.8398 bits (133), Expect = 1.880e-7 Identity = 95/457 (20.79%), Postives = 194/457 (42.45%), Query Frame = 3
Query: 1188 IQKFVSKLELLEGATFISNAL---RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD-IAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD---ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL-VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY--RADVAKIVVLFTDKLPTNQKDSL-SEAEILKGDGVKII 2525
I + V+ L G I L RRS E GR GAKK ++++T+ ++ + + A L + IR+I + LG ++L K++ + +D+ +++ +T+ IA + R+ KL +A+ + + + + K + ++ G +S+V+ ++ V+ F+ + D +++ ++ ++ G++ + K L F + + ++++ VV++ S + +AA DL+ +G+ + + +D+ EL T PN +Q+ L A+K S D++F + S N + + ++ ++Q R + + + + R D + + + I +R N +G L+ AL A VF R +K +VL TD KD + S + L+ V II
Sbjct: 5061 IDELVASLRRASGRPDIVKTLTMARRSLEN----GRAGAKKVMVILTNQQSSNLPSELKAAAIPLDRAEIRIITVPLGPDANTNELAKITPVK-----KDLLRVSPGASTEQIATAVRNALRRDRLRVPKLQLAIAISAGAIKAVENFQKMKEVVKSIVSRY--GSFNISYSVVAMGSDPAVQIAFSKTFPDDDALMRAIDGVQWTFGYS-LAKGLRELKSVFASEGQETRKVAVVIMDRRSSSSVSEVKRAALDLEVAGIKVIPVAFEGYSDKSELG--KTTPN---KQNLIARMPLESPDTTAEKIVRKAVKVLPPISELDLVFAI-SSVANPVANFPKMLDIIKQSVQQYGQERIHFYVIV--FGSRAEVKLRFDASLSDDQVKKKISGIRPNDDGDSLDKALELASDVFGAASAKRPGASKTLVLITDHSSRLPKDVIQSSRQALENASVNII 5497 HSP 23 Score: 51.6026 bits (122), Expect = 3.767e-6 Identity = 111/550 (20.18%), Postives = 234/550 (42.55%), Query Frame = 3
Query: 993 QQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQID-IKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLE---LLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVE--AEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQ--IPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLL--FAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD---DILKKVESLKHVGGFTRIDKALNLASRSFY-LKSKNSKRLVVLLTNGLSMERVQTIQAAYD-LQESGVDIATIGLGDKADREELKILS--------TFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETT------TEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFK--IDYRADVAKIVVLFTDKLPTNQKDSLSEA----EILKGDGVKIIFVGL 2537
+ +D++F + +SS L +EL +I + +D +K S +G + D + + P+ K + S+++ L+ G + AL ++ EF + GR AKK +++V D SP +++ L ++VIV+ +G Q ++ D+S + D + Q + + + S D ++ S+ Q + ++L K + ++ GI R+ ++ F N +F ++ D++K + +L+ G R+ AL+ A R F K +K++++ + +I++ D L GV I + +G + +++K +S + +P D +K + V ++ P KP DI F + S+ +++ + +++++ L I + +T E R ++ I ++ N G + +ALNR +Q FK R + K +++ TD +N +D + + E ++ DG+++++VG+
Sbjct: 4436 KPMDVLFAVSSSSPQNLLKAKEL--IKRMIDTYGMDKLKISYIG---FGD--KTTSPISFKDKFPSAEDLKSRIDVISLVPGRPNVLEALDKARNEFETGGRSNAKKILVIVVD--KRSPNTNEQIKDAVTPLADRDVKVIVVTVGEDADPQQGTSLTG--------DISAVIKADNSSEPDQLVQKIVKEGSKEVSPVRDADIVFALSATGQQRDQSFRMLQEAVKGLVARY--GIGSVRYGVIVFGNTVSTAVDFGDQFNSASDLMKAISNLERKDGGPRLGLALDRAKRQFSNTNRKGAKKILIFTLDQKPTTDEASIRSKSDELNSMGVKIIPLLVGSEIGPQDVKPVSGDTKNIEVSVTDP---PDHVTDKLYKQIGEAVKEESQP--KPSL-----DIGFAISASSADSDANFAKMKNIIKAVLDIYGTEGVHYTIVTFGQTAKTVVQNSKGAELRAGLPTLATELKRLIDRITKNPEGTSIVSALNRIKQSFKEATSSRPNTKKTLIIMTD---SNHEDGVDDVIIAMEGVEDDGIQVLYVGV 4953 HSP 24 Score: 51.2174 bits (121), Expect = 4.419e-6 Identity = 72/360 (20.00%), Postives = 163/360 (45.28%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFS---QVGVLIYSDILEMSLPLGSK-QNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICS----AKLDIALIVDCSS--QVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTH----IYDDILKKV--ESLKHVGGFTRIDKALNLASRSFYLKSKNSK----RLVVLLTNGLSMERVQTIQAAYD-LQESGVDIATIGLGDKADREELKILSTFPN 2018
D++F L A S + LA N IKS+ ID S + GV+++ + + + + ++ ++I++++ + + + + +L + + F+ +GR A+ ++++TDG S + E L +M + +I + G QL +S + Y + + D +++ Q R+ + + D+A ++ + Q + Q++ I + +G T +SLV++ + V +F D L++ S+ G + KAL +++R + K S+ R++V++++ S + ++AA L + + + ++ LG + + ELK ++ P+
Sbjct: 5728 DMLFALSAGSGDPV---SNLARMKNTIKSI-IDRYGSTSIRYGVIVFGGVPVIKMGFEKEYRSDSDIKRYLDSINREKQGSSLHRSLEEARKVFVQQGRPDAQHVLVIMTDGQSESLPDEIETARNPLDQMNVTIIPVAFGDESDPAQLRVATSDDRYLIVSKTT----VDPSELGQQIMD-----RLSAESKVQETDLAFVISAAGPDAQQTFSKIKQII--GLVISNYSIG--DTLYSLVTYGDRPVTHVQFKDSKKFPSDSELRQFIRNSVSKPSGAADLYKAL-MSARDLFSKEAGSREKAARVMVVISDRESDSSLMDVKAAVSGLIQDNIRLISVALGGQGNENELKSVAPVPD 6069
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO34390 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPH4]) HSP 1 Score: 115.546 bits (288), Expect = 7.220e-26 Identity = 175/740 (23.65%), Postives = 325/740 (43.92%), Query Frame = 3
Query: 2097 AIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDE-----TTTEF---RLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRA-RQVF--KIDYRADVAKIVVLFT------DKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT---KGREDVPKVLVVLTDGANDIGESPQISAV-KAKLGGIKIISVGVGQQISEKELNDIAS--------SGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTK---LTVVDKENT-RVAILKFNRGNYIDSGFKEDYKNFES---------SIEDMTTSTNGVFIGKALTSARVLF---KSDTRKNATKTVIIIIDGEITDRNETFEEANL-LKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARK--EFGLDQFTEKYAMISAVKRIIF-DDGATDIGEALRECKNELLNS---TRKEVPKVIVLVTDG 4157
A+K + C++ D+ ++D S + + W + + IS +RIS+ +S + + EF ++ A KR I + KQ S G G ++ AL A + +F + R D K+ +L T D+ P+ Q +++ +L GV I VG+ + N+AS + I + + + + F Q K LD+++ I S +LG + QI+L FVN ++ D+ +T +G+ TF+ +++ +D +E + + IN I I AI A DY FT R+ +PK V+ + + + A K K G++++ VG+ I++ + + + S S Y+ FT + A T+ N AC +P D+ F+M +S + +SE++ DF+K K ++ D EN+ AI ++ + Y +G K ++NF S S+ M++S +G L A F K+ R+ + +I++ + +E E A + L K+ ++ +G T + L+ +AS +F VD L + ++ LC K K ++++F ++S+ +G + F+ R+V + D++ + + T+ A+ +F K AV+ +F +G EAL+ + S +R+++ K IVL+ G
Sbjct: 82 AVKQERCAAAIDLAILLDASEAISPQEWSKLLKFTADLMDYYGISEDGTRISVATFSTDVDIVLSFNEFSGVEMNAASVKRGI-LGAKQ--SRGPGLRIDKALKAADKDLFNRRFGMREDQKKVCLLVTSGAQTKDQGPSTQLGTVTA--LLSARGVDIYAVGVGDGVDSSELRNIASTEDFIYTASSFEEINK-------VLEPFGTVQCSGCKKPLDLVLGIPTSRTLGDDFKQIKLFFVN-LIALFDISPSKTHVGVITFSESAQMSLHMDKMYEPRTVTRFINDITPTGDQMRIDRAIDTAGDYGFTIYGGVRQTMPKTFVLFAPTNSSSSAAANMKAAEKLKSLGVRLLIVGLTADINKVDFSSVVSQPSRKFLFSSAAYD--GFTSVRHDMASTLCN-ACFVPI---------DVAFIMDNSESVSSEKY----DFVKRFVKDVIMSYADAENSANFAIGQYAK--YFQTGTKR-FRNFRSMEELDEVINSLRQMSSSAERN-VGAGLRGAANEFFQVKNGMRQGLPRFLIVLASANPSASSEAIESAVVNLDKENVRRIAVGFTEDATPGFLRMLASDPSLMFRVDEPKKLDKVMMELTPMLCQACK--------KSMEILFLVESTKFVGEHGFNRVKHFITRLVENFDVSGTTITLAMATYADSAQPVVQFSDSAAWRKAEFQKAVQGALFLGEGRVRTAEALKFADRRIFTSRFGSREDIKKTIVLIGSG 780 HSP 2 Score: 90.5077 bits (223), Expect = 3.262e-18 Identity = 154/725 (21.24%), Postives = 310/725 (42.76%), Query Frame = 3
Query: 999 LDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPL----GSKQNKTEIQKFVSKLELLEGATF-ISNALRRSGEEFISKG---REGAKKTVILVTDGA---DNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTE----TYYPIEDMSK-MALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQL-LFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD--ILKKVESLKHVGGFTRIDKALNLASRSFY-----LKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYS---DETTTEFRLDEAFTKRD-IYVAIKQLRSNGN---GGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYENLAX-XXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTF--NSEVYLNVPIDSNFENADMQKEINKIPFI-EGNTAIGWAIGNATDYLFTK---GREDVPKVLVVLTDG-ANDIGESP 3050
+DL +LDAS +++ W + L FT +++ I +++ V +S +++ L G + N +++ + + G I AL+ + ++ ++ RE KK +LVT GA D P+ + L G+ + +G+G V +L ++STE T E+++K + F T + C LD+ L + S L + Q+ LF I F++ +KT +++F+ A + +Y+ + + + + G RID+A++ A + ++ K V+ S ++AA L+ GV + +GL AD ++ S P + F SV+ +T C D+ F++D S ++E +D ++ + + S + ++ +I Y+ T FR + + D + +++Q+ S+ G L A N QV K R + + +++ P+ +++ A + L + V+ I VG ++++ F LAS +++ K+DK ++ M+C A K K ++++ ++ + +G F F+ R+V+N D+ + + T+ +++ + + + A+ QK + F+ EG A+ A +FT RED+ K +V++ G N++ P
Sbjct: 92 IDLAILLDASEAISPQEWSKLLKFTADLMDYYGISEDGTRISVATFSTDVDIVLSFNEFSGVEMNAASVKRGILGAKQSRGPGLRIDKALKAADKDLFNRRFGMREDQKKVCLLVTSGAQTKDQGPSTQLGTVTALLSARGVDIYAVGVGDGVDSSELRNIASTEDFIYTASSFEEINKVLEPFGTVQCSG-----------CKKPLDLVLGIPTSR--TLGDDFKQIKLFFVNLIALFDISPSKTHVGVITFSESAQMSLHMDKMYEPRTVTRFINDITPTGDQMRIDRAIDTAGDYGFTIYGGVRQTMPKTFVLFAPTNSSSSAAANMKAAEKLKSLGVRLLIVGL--TADINKVDFSSVVSQPSRKFLFSSAAYDGFTSVRHDMASTLC---NACFVPIDVAFIMDNSESVSSEKYDFVKRFVKDVIMSYADAENSANFAIGQYAKYFQTGTKRFRNFRSMEELDEVINSLRQMSSSAERNVGAGLRGAANEFFQV-KNGMRQGLPRFLIVLASANPSASSEAIESAVVNLDKENVRRIAVGFTEDATPGFLRMLASDPSLMFRVDEPKKLDKVMMELTPMLCQACK--------KSMEILFLVESTKFVGEHGFNRVKHFITRLVENFDVSGTTITLAMATYADSAQPVVQFSDSAAWRKAEFQKAVQGALFLGEGRVRTAEALKFADRRIFTSRFGSREDIKKTIVLIGSGRYNEVPTQP 789 HSP 3 Score: 65.855 bits (159), Expect = 1.294e-10 Identity = 144/653 (22.05%), Postives = 271/653 (41.50%), Query Frame = 3
Query: 1527 CQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKH--------VGGFTRIDKAL-----NLASRSFYLKSKNSKRLVVLLTNGLSME------RVQTIQAAYDLQESGVDIATIGLGDKADREELK-ILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RADVAKIVVLFTDKLPTNQKDS----LSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXM---ICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLR-KDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKI--------PFIEGNTAIGWAIGNATDYLFTKG--REDVPKVLVVLTDGANDIGESPQISAVKAKLG--GIKIISVGVGQQISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTK 3353
C+ + C+A +D+A+++D S + EW L F ++ + + + TR S+ +F+ + + F + + S+K G RIDKAL +L +R F ++ ++ K++ +L+T+G + ++ T+ A L GVDI +G+GD D EL+ I ST + F+E +LE V C C D++ + S + + I+ + + +IS + + + +I +S+ +D+ + R + I + G+ ++ A++ A Y R + K VLF PTN S + AE LK GV+++ VGL+ + + ++ S+ S + + D +M +C+A C + +DV +D S S+ E + DFV R V+++ + D F F N +E++++ E N G G A ++ K R+ +P+ L+VL AN S I + L ++ I+VG + + L +AS + V L+K + ++ CQ CK + +++F++ S+ F ++ F+ L +
Sbjct: 79 CRKAVKQERCAAAIDLAILLDASEAISPQEWSKLLKFTADLMDYYGISEDGTRISVATFSTDVDIVLSFNE-FSGVEMNAASVKRGILGAKQSRGPGLRIDKALKAADKDLFNRRFGMR-EDQKKVCLLVTSGAQTKDQGPSTQLGTVTAL--LSARGVDIYAVGVGDGVDSSELRNIASTEDFIYTASSFEEINKVLEPFGTVQC--------SGCKKPLDLVLGIPTSR-TLGDDFKQIKLFFVNLIALFDISPSKTHVGVITFSESAQMSLHMDKMYEPRTVTRFINDITPTGDQMRIDRAIDTAGDYGFTIYGGVRQTMPKTFVLFA---PTNSSSSAAANMKAAEKLKSLGVRLLIVGLTADINKVDFSSVVSQPSRKFLFSSAAYDGFTSVRHDMASTLCNA-------CFV-PIDVAFIMDNSESVSSEKY----DFVKRFVKDVIMSYADAENSANFAIGQYAKYFQTGTKRFRNFRSMEELDEVINSLRQMSSSAERNVGAGLR-GAANEFFQVKNGMRQGLPRFLIVLAS-ANPSASSEAIESAVVNLDKENVRRIAVGFTEDATPGFLRMLASDPSLMFRVDEPKKLDKVMME-LTPMLCQ------ACKKSMEILFLVESTKFVGEHGFNRVKHFITRLVE 694 HSP 4 Score: 50.8322 bits (120), Expect = 5.440e-6 Identity = 41/145 (28.28%), Postives = 66/145 (45.52%), Query Frame = 3
Query: 3783 CDVI--KGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATD-------IGEALRECKNELLN---STRKEVPKVIVLVTDGVSTGGLSP 4181
C+++ K +E +DL LD+S +I P W L F ++ I+ + T++ V TF+TD ++F+ ++VKR I GA I +AL+ +L N R++ KV +LVT G T P
Sbjct: 75 CELLCRKAVKQERCAAAIDLAILLDASEAISPQEWSKLLKFTADLMDYYGISEDGTRISVATFSTDVDIVLSFNEFSGVEMNAASVKRGIL--GAKQSRGPGLRIDKALKAADKDLFNRRFGMREDQKKVCLLVTSGAQTKDQGP 217
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO47555 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM80]) HSP 1 Score: 99.7525 bits (247), Expect = 6.823e-22 Identity = 94/387 (24.29%), Postives = 165/387 (42.64%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLALYWDQELA----FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD-----IAQDACQ-IPKPKR---------------------------ICSAKLDIALIVDCSSQVQLHEWVN-QLL--FAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS--KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP 2021
++D+ F+LD S S+ Y F N +KS + S G++++S + + I V+ + T+ L + ++ S R G + +I++TDG + P A+ L+ MG+ + +G+G + + QL+ M S P D A FD D I AC I K + +C AK+D+ ++D S V+ + N Q F +F++ F + + + F LV F++++ VE +F YD I V + + G T K L LA Y S R+++++T+G+S + V A L++ GV+I +G+G D+ +L + + P P
Sbjct: 5 KVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATKYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGP---AKALRDMGVEIFALGIGKNYDQGQLDSMGSD----PKPDHVVTADFDKLDPVIQTIKDKACNGIDKEQTESHSASPVDDGALKTKNQNRFRSSSVTVCKAKVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATNYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGPAKA--LRDMGVEIFALGIGKNYDQGQLDSMGSDPKP 382 HSP 2 Score: 97.4413 bits (241), Expect = 3.244e-21 Identity = 97/413 (23.49%), Postives = 187/413 (45.28%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSL---GPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG-REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNA--KTISNEACQIPQKP----------------------------TICKAAGDLIFVM---GSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN----GVFIGKALTSAR-VLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS---PDKALFVDNFSNLQRYNAYISSELCDVIK 3797
K+DV +D S S+ NFQ +F+N+ V++ + KD + G+ F+S+ + D +++ A + +N + T G + A D L++ R VP++L+V+TDG + + A++ G++I ++G+G+ + +L+ + S + +V T D +K + +TI ++AC K T+CKA D+ F++ GS F++ +F+ K +V K+++ ++ F+ + ++ F + Y +SI +TN G + GK LT A+ L+ + R + +I++ DG +D + A L+ G+++F +G+ + L S+ S PD + D F L I + C +K
Sbjct: 5 KVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATKYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGPAKALRDM--GVEIFALGIGKNYDQGQLDSMGSDPKPDHVVT-ADFDKLDPVIQTIKDKACNGIDKEQTESHSASPVDDGALKTKNQNRFRSSSVTVCKAKVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYD--AASITAAVNATNYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSD--DVAGPAKALRDMGVEIFALGIGKNYDQGQLDSMGSDPKPDHVVTAD-FDKLDPVIQTIKDKACKGMK 409 HSP 3 Score: 80.8777 bits (198), Expect = 9.887e-16 Identity = 79/348 (22.70%), Postives = 155/348 (44.54%), Query Frame = 3
Query: 3249 ICKAAGDLIFVM---GSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN----GVFIGKALTSARV-LFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS---PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKE---------------------------CSTKQLDLMFALDSSSSI---GPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS-TRKEVPKVIVLVTDGVST 4166
+CKA D+ F++ GS F++ +F+ K +V K+++ ++ F+ + ++ F + Y +SI +T G + GK LT A+ L+ + R + +I++ DG +D + A L+ G+++F +G+ + L S+ S PD + D F L I + C+ I E C K +D+ F LD S S+ +Q +F+ + V+ ++ + + G+V F++D+ EF D + ++ +AV + T G+ L K++L ++ R VP++++++TDG+S+
Sbjct: 1 MCKAKVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYD--AASITAAVNATKYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSD--DVAGPAKALRDMGVEIFALGIGKNYDQGQLDSMGSDPKPDHVVTAD-FDKLDPVIQTIKDKACNGIDKEQTESHSASPVDDGALKTKNQNRFRSSSVTVCKAK-VDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATNYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISS 342 HSP 4 Score: 80.4925 bits (197), Expect = 1.328e-15 Identity = 87/373 (23.32%), Postives = 171/373 (45.84%), Query Frame = 3
Query: 1551 ICSAKLDIALIVDCSSQVQLHEWVN-QLL--FAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS--KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP--FLRQDFXXXXXXXXXXXXXXCK-----------TTP----AIKPQ-----------TCSSNGDIIFVVDKSTDNTNEIW-----DGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRAR-QVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKN 2546
+C AK+D+ ++D S V+ + N Q F +F++ F + + + F LV F++++ VE +F YD I V + K+ G T K L LA Y S R+++++T+G+S + V A L++ GV+I +G+G D+ +L + + P P + DF + +++ +C +P A+K + C + D+ F++D S + E + ++ + ++S +S+ S ++ +S ++ EF+ D+ + I A+ G G L A+ ++ R+ V +I+++ TD + ++ D A+ L+ GV+I +G+ KN
Sbjct: 1 MCKAKVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATKYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGPAKA--LRDMGVEIFALGIGKNYDQGQLDSMGSDPKPDHVVTADFDKLDPVIQTIKDKACNGIDKEQTESHSASPVDDGALKTKNQNRFRSSSVTVCKAKVDVGFLLDGS--GSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATNYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSD--DVAGPAKALRDMGVEIFALGIGKN 367 HSP 5 Score: 52.7582 bits (125), Expect = 8.156e-7 Identity = 45/189 (23.81%), Postives = 78/189 (41.27%), Query Frame = 3
Query: 996 QLDLIFVLDASSSLALYWDQELA----FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG-REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD-----IAQDACQ 1532
++D+ F+LD S S+ Y F N +KS + S G++++S + + I V+ T+ L + ++ S R G + +I++TDG + P A+ L+ MG+ + +G+G + + QL+ M S P D A FD D I AC+
Sbjct: 225 KVDVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNATNYPGMGTYAGKGLTLAKDDLYSAPVRSGVPRILIVMTDGISSDDVAGP---AKALRDMGVEIFALGIGKNYDQGQLDSMGSD----PKPDHVVTADFDKLDPVIQTIKDKACK 406 HSP 6 Score: 50.8322 bits (120), Expect = 3.180e-6 Identity = 37/143 (25.87%), Postives = 68/143 (47.55%), Query Frame = 3
Query: 150 DIAVILDSSSSI---TNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
D+ +LD S S+ G FQ IN+F+ F++ +++ + + EF F H + ++ AV K G + G+ L D+ S + RIL++++DG ++D+ V A +++ GV I+ + IG
Sbjct: 7 DVGFLLDGSGSVEFYAKGNFQRCKNFINKFVKSFMVSKDDSHFGLVLFSSDSNVEFKFDDHYDAASITAAVNA-TKYPGMGTYAGKGLTLAKDDLY-SAPVRSGVPRILIVMTDGISSDD--VAGPAKALRDMGVEIFALGIG 145
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO26384 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TC56]) HSP 1 Score: 93.2041 bits (230), Expect = 8.760e-20 Identity = 103/424 (24.29%), Postives = 189/424 (44.58%), Query Frame = 3
Query: 3267 DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAI--------LKFNRGNY---------IDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIG---------LTGKTSESDLKSVASPD----------------------KALFVDNFSNLQ-------RYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIG---PDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRIL--DFKTLKDYDTTFITRESC 4358
DL+FV+ S + T++ F + DFMK + V + T V + + FN Y +DS K D +++ + F G L R++ + N +I++ DGE D + E + +L++D ++ +G L SE + V + D + L +++ ++ Q +++ S+ C+ G E S +DL F LD S+SI P +Q +F+ IV+ I++ T VG+V ++ + F D++++ +++ A+ + G T GEAL+ K++L ++ + VPKV++++TDG S+ + E S +K G+ I +G+GD PS+L +++S + FK L D I R++C
Sbjct: 1 DLVFVIDGSNDLTADAFTKAKDFMKKVYNNFPVSESATHVGLALSGCTSKVMFNLIEYFTKPALDSAVDSARKPDPYEVTGAVDKIKYPGGSTFTGAGL---RMV-----KSNVPHILIVMTDGESHD--DVAEPSRVLREDNTIIYAVGEGYYFKLKQLNEMASEPKINHVFTADFGQMNTIVEDIKDKACSGKKIRRQLLLNHLNDQQSDVHSPHNHDSCDHSQFCEYQAGLT-ENSRLAVDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSFFTQLMFNFDKYSDSASIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSDKV--EAPSKALKDEGVVIFAVGVGDQIDPSELNVMASDSKSDHVFKAKFKEL-DRLVDLIKRKAC 410 HSP 2 Score: 82.0333 bits (201), Expect = 3.979e-16 Identity = 62/183 (33.88%), Postives = 98/183 (53.55%), Query Frame = 3
Query: 2697 LDVIVTIDKSTSLG---PENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNV--KTFTDLEKFNAKTISNEAC 3227
+D+ +D STS+ P NFQ+ +F+ IV++ + +RT +G+ ++ L D ++A + K IN + +G T G A+ A LF VPKVL+VLTDG ++D E+P A+K + G+ I +VGVG QI ELN +AS + +V F +L++ I +AC
Sbjct: 232 VDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSFFTQLMFNFDKYSDSASIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSDKVEAPS-KALKDE--GVVIFAVGVGDQIDPSELNVMASDSKSDHVFKAKFKELDRL-VDLIKRKAC 410 HSP 3 Score: 76.6406 bits (187), Expect = 2.272e-14 Identity = 100/431 (23.20%), Postives = 175/431 (40.60%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGI--------FTFNSEVYLNVP-IDSNFENA------DMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFN--AKTISNEAC---------------------QIPQKPTIC----------------KAAGDLIFVM-GSSA--NKTSERFKQMSDFMKSLTKLTVVDKENTRVAI--------LKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKA--LFVDNFSNLQRYNAYISSELC 3785
D++ ID S L + F DF+ ++ N + + T +G+ FN Y P +DS ++A ++ ++KI + G+T G + + +VP +L+V+TDG +D+ E ++ + I +VG G K+LN++AS + +V T D + N + I ++AC P C + A DL FV+ GS++ N F+ + +FM ++ K + E T V + L FN Y DS + +I G G+AL A+ + ++ K +I++ DG +D+ E +A LK +G+ +F +G+ + S+L +AS K+ +F F L R I + C
Sbjct: 1 DLVFVIDGSNDLTADAFTKAKDFMKKVYNNFPVSESATHVGLALSGCTSKVMFNLIEYFTKPALDSAVDSARKPDPYEVTGAVDKIKYPGGSTFTGAGLRMV--------KSNVPHILIVMTDGESHDDVAEPSRV----LREDNTIIYAVGEGYYFKLKQLNEMASEPKINHVFT-ADFGQMNTIVEDIKDKACSGKKIRRQLLLNHLNDQQSDVHSPHNHDSCDHSQFCEYQAGLTENSRLAVDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSFFTQLMFNFDKYSDSA------SIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSDKVEAPSKA--LKDEGVVIFAVGVGDQIDPSELNVMASDSKSDHVFKAKFKELDRLVDLIKRKAC 410 HSP 4 Score: 56.225 bits (134), Expect = 6.254e-8 Identity = 41/155 (26.45%), Postives = 77/155 (49.68%), Query Frame = 3
Query: 999 LDLIFVLDASSSL--ALYWDQEL--AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSS 1451
+DL FVLD S+S+ A + +L F N++KS +I + + VG+++YS ++ + I K ++ + +G T AL+ + + K +I++TDG + K E ++ LK G+ + +G+G + +LN M+S
Sbjct: 232 VDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSFFTQLMFNFDKYSDSASIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSD---KVEAPSKALKDEGVVIFAVGVGDQIDPSELNVMAS 383
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Match: EDO40688 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6N8]) HSP 1 Score: 83.9593 bits (206), Expect = 1.283e-18 Identity = 57/155 (36.77%), Postives = 87/155 (56.13%), Query Frame = 3
Query: 2697 LDVIVTIDKSTSLG---PENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIAS 3149
+D+ +D STS+ P NFQ+ +F+ IV++ + +RT +G+ ++S L D ++A + K IN + +G T G A+ A LF VPKVL+VLTDG ++D E+P A+K + G+ I +VGVG QI ELN +AS
Sbjct: 2 VDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSSFTQLKFNFDKYSDSASIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSDKVEAPS-KALKDE--GVVIFAVGVGDQIDPSELNVMAS 153 HSP 2 Score: 74.3294 bits (181), Expect = 3.384e-15 Identity = 49/154 (31.82%), Postives = 92/154 (59.74%), Query Frame = 3
Query: 3828 LDLMFALDSSSSIG---PDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSS 4280
+DL F LD S+SI P +Q +F+ IV+ I++ T VG+V +++ + +F D++++ +++ A+ + G T GEAL+ K++L ++ + VPKV++++TDG S+ + E S +K G+ I +G+GD PS+L +++S
Sbjct: 2 VDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSSFTQLKFNFDKYSDSASIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSDKV--EAPSKALKDEGVVIFAVGVGDQIDPSELNVMAS 153 HSP 3 Score: 56.6102 bits (135), Expect = 4.501e-9 Identity = 41/155 (26.45%), Postives = 77/155 (49.68%), Query Frame = 3
Query: 999 LDLIFVLDASSSL--ALYWDQEL--AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSS 1451
+DL FVLD S+S+ A + +L F N++KS +I + + VG+++YS ++ + I K ++ + +G T AL+ + + K +I++TDG + K E ++ LK G+ + +G+G + +LN M+S
Sbjct: 2 VDLAFVLDGSTSINNADPGNFQLLKNFMINIVKSFKISSERTHVGLVLYSSFTQLKFNFDKYSDSASIVKAINTTDYPKGGTRTGEALKMAKSQLFGASMRSVPKVLIVLTDGRSSD---KVEAPSKALKDEGVVIFAVGVGDQIDPSELNVMAS 153
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: COL6A6 (collagen type VI alpha 6 chain [Source:HGNC Symbol;Acc:HGNC:27023]) HSP 1 Score: 237.269 bits (604), Expect = 1.532e-62 Identity = 276/1110 (24.86%), Postives = 504/1110 (45.41%), Query Frame = 3
Query: 1002 DLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALR-RSGEEFI----SKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMA------LFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKT-----------TPAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF--KIDYRAD-VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFK-EDYKNFES---SIEDMTTSTNGVFIGKALTSARVLFKSDTRKNA----TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKA--LFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL---RECKNELLNSTRKE-VPKVIVLVTDGVS 4163
D++F++D S S+ + Q F + ++ S + ++G+ YSD L + QN ++ +++++L G T L E F+ S+ G + +++TDG + +AEKLK+ GI + IG+ +++ QL +++ + Y + D S + LF + D+ + + DI +VD SS + + + LF + + ++G +K R L +++E E +D +L +E L + G T KA++ ++ + S+ ++ V++T+G S + V+ + A L+E GV + I A+ EL +++ P + F+ + F E L K KV C + AIK + C + DI F++D S + + + ++ +Q+ I R+ ++ Y+D EF L+ ++ A++ ++ G G + AL +F D R V + +V+ TD +++ S AE L+ GV I +G+ + E K + + + ++ K+++ IC A + C D++ ID S S+ P++++ DF+ ++ + ++ +G+ F+S + S F DM + IN + + G T G A+ + Y + GR + + LVV+TDG A D P A++ K G+KI ++GV + + L S Y+ + F L+ + ++ + C PQ+ D+IF++ S + + E FK M FM S+ T V + TR +L ++ N FK +K + +I + + + GKAL F ++ A + +++I DG+ TDRN E + L+ G+ +F+IG+ G + + L+ +A DK+ FVDNF+ L+ IS ELC+ T C +Q DL+F +D S SI + +F +V+ I +VG+ F++ + EF L+QF+E + + + G T+IG AL RE S R E + + +VL+TDG S
Sbjct: 22 DIVFLVDGSWSIGRPNFQQVRHFLSTLVSSFDVSPDHVRIGLAQYSDDSRTEFLLRTFQNNRDVLQYIARLPYKGGGTMTGKGLDFLLQEHFVEAAGSRASSGVTQIAVVITDGKSQDEV---KSQAEKLKQRGIILYAIGIKNAILT-QLKEIADEQNIYTVSDFSAPSQGTDRFLFVVS--GSDSYLFFSAECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVE--EPAQQLREQGVIVFGI-----ANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIK-EGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKS--PAEKLRSKGVTIYTIGVKEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSA---DGEVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQDEVRGPA-EALREK--GVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLES-LLALQLCD-PQRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYS--NDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEPSEALRSKGVNIFSIGVKG-ANMTQLEIMAGQDKSKVFFVDNFAALEGLYKNISKELCN---STKPVCEKQQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGGGTNIGLALKSIREYFETSRGSRRSEGISQNLVLITDGES 1101 HSP 2 Score: 181.8 bits (460), Expect = 1.617e-45 Identity = 232/978 (23.72%), Postives = 439/978 (44.89%), Query Frame = 3
Query: 1002 DLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNAL-----RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTT-------DIAQDACQIP------KPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK-----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP-FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF--KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GRED--VPKVLVVLTDG----ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVI 3794
D++F++D SSS+ + +++ F V+ L I +VG+ YSD L + + + + +L G T A+ + EE S+ R+ + +++TDG + +P A++L++ G VIV G+ Q+ E + ++ +++ +I + A + K + + + DI ++D S + ++ + + F FI+ F +G R +V +A+ +E D ++ V +++ GG T +AL S + K+K+S+ +V++T+G S + V++ A L+ GV I TIG+ +AD++EL+ +S P F DF + +K + C + C +GD++F++D S + ++ ++ M + I R + ++ +S EF L F+K D+ AI ++ G G AL Q F + R + + +V+ TD Q + AE L+ GVKI +G+ + E S + + + + + +CD Q +C ++ D+I +D STS+ ENF+ F+ +V + ++ TR G+ ++++ + ++ ++ K I+ + G+T G A+ + + GR + VP++L+V+TDG N++ E + A+++K G+ I S+GV G +++ E+ + + V F LE K IS E C KP K DL+F++ S + + + M +F L K + ++ RV + +F+ + + + F ED + E +E MT + G IG AL S R F+ S + ++ +++I DGE D E + A+ L+ GI++F IG+ L+ +P + V NF +L+ + LC V+
Sbjct: 217 DIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVEEP---AQQLREQG--VIVFGIANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMG-SLFDKAKDSRGHKVPEYLVVITDGKSSDEVKS--PAEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSA----DGEVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDG--QAQDEVRGPAEALREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLESLLALQLCDP----QRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEPSE--ALRSK--GVNIFSIGVKGANMTQLEIMAGQDKSKVFFVDNFAALEGLY-KNISKELCN-STKPVCEKQQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILE-MTQTGGGTNIGLALKSIREYFETSRGSRRSEGISQNLVLITDGESQDDVE--DPADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNFGSLENIKQKV---LCVVM 1163 HSP 3 Score: 172.94 bits (437), Expect = 8.392e-43 Identity = 199/794 (25.06%), Postives = 368/794 (46.35%), Query Frame = 3
Query: 2127 GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RAD--VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMIC--DAFKQRQDECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAK-------TISNEACQIPQKPTICKAA------GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKN-ATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNST--RKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFI-----TRESCKAGKKA 4376
DI+F+VD S + +R + T + S ++S RI + YSD++ TEF L RD+ I +L G G L+ Q ++ RA V +I V+ TD +Q + S+AE LK G+ + +G+ KN+ + +A +++ + + + + ++ ++ D++ EC M + D++ +D S+S+G +NF+ F+ +V LD+ D+ R+G+ ++ E Y + +F+ + + I ++P+ G T G AI FT + R+ VP++ VV+TDG ++D+ E Q +L +I G+ +E EL IAS + VK+F +L K I +A + + + K D+ F++ S + FK M +F+ + + + R+ ++K+ ++ G + D +N E+++ + G G+ALT LF D+R + + +++I DG+ +D E A L+ G+ ++TIG+ + + +L+ ++ P + FV++F L N + D+ + C DL+F +DSS SI P ++ DF++ ++ I N VGV+ F++ + EF L F K M+ A+ + G T G+AL ++ R + + +V+VTDG + + E+ +++ G+KI IG+ D+ + L+I S Y DF LKD ++ + CK ++A
Sbjct: 21 ADIVFLVDGSWSIGRPNFQQVRHFLSTLVSSFDVSPDHVRIGLAQYSDDSRTEFLLRTFQNNRDVLQYIARLPYKGGGTMTGKGLDFLLQEHFVEAAGSRASSGVTQIAVVITDG--KSQDEVKSQAEKLKQRGIILYAIGI-KNAILTQLKEIADEQNIYTVSDFSAPSQGTDRFLFVVSGSDSYLFFSAECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVEEPAQ------QLREQGVIVFGIA---NETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSD--EVKSPAEKLRSKGVTIYTIGVK-EADKKELEEISGDPKRMFFVNDFDAL---NPIKDKVITDICSADGEVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQDEVRGPAEA--LREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLESLLALQLCDPQRDCKKTERA 794 HSP 4 Score: 172.94 bits (437), Expect = 8.681e-43 Identity = 213/933 (22.83%), Postives = 415/933 (44.48%), Query Frame = 3
Query: 1563 KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIG--------LGDKADREELKILSTFPNPFLRQD---FXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALN-------------RARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSE-AEILKGDGVKIIF--------VGLSKNSPYN---FYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGV-------FIGKALTSARVLFKS--DTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECS-TKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKE--VPKVIVLVTDGVST 4166
K DI +VD S + + F + F++ + R L +++++ E + D+L+ + L + GG T K L+ + ++++ S+ ++ V++T+G S + V++ A L++ G+ + IG L + AD + + +S F P D F S C ++ DI+F+VD S+ + ++ +R + + L+I R+ + YSDE EF L + F + AI++L + A++ RARQ V +I V+ TD + D + E A+ L+ GV I+F + ++ + P ++ A Q + + ++ ++ D+ ID S S+ P +F+ ++F+ +Q + R+G+ + L +++ + +++ + I G T G A+ LF K ++ VP+ LVV+TDG ++D +SP A K + G+ I ++GV ++ +KEL +I+ + + V F L K I++ C + +CK +GDL+F++ SS + + +++M DFMK + + + + V +L+F+ + D ++F S EDM + NG+ G ALT+ F S R + + ++++ DG+ D E A L++ G+K++ IG+ + L+ S D+ +F L+ + ++ +LCD ++C T++ D++F +D S+SI + +++ F+ +V + N T+ GV+ ++ D + F L+QF + ++ A+ + G T G+AL+ C N R E VP++++++TDG +T
Sbjct: 20 KADIVFLVDGSWSIGRPNFQQVRHFLSTLVSSFDVSPDHVRIGLAQYSDDSRTEFLLRTFQNNRDVLQYIARLPYKGGGTMTGKGLDFLLQEHFVEAAGSRASSGVTQIAVVITDGKSQDEVKS--QAEKLKQRGIILYAIGIKNAILTQLKEIADEQNIYTVSDFSAPSQGTDRFLFVVSGSDSYLFFSAEC---------AMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQ--------RVPQIAVVITDG---DSSDDVEEPAQQLREQGV-IVFGIANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRAL-TFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKSP---AEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITD-ICSADGE--VCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEF----DLRSFFSK-EDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQD--EVRGPAEALREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLESLLALQLCD----PQRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDAT 910 HSP 5 Score: 117.857 bits (294), Expect = 6.462e-26 Identity = 123/534 (23.03%), Postives = 247/534 (46.25%), Query Frame = 3
Query: 2682 CTMK-KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLF---------TKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFT----DLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLF-----KSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS--PDKALF-VDNFSNLQRYNAYISSELC-DVIK----------GTNKEC-STKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALR---ECKNELLNSTRKEVPKVIVLVTDGVST 4166
CT + K D++ +D S S+G NFQ F++ +V + D+ D RIG+ ++ + + + N D+ + I ++P+ G T G + D+L ++ V ++ VV+TDG + + + A K K GI + ++G+ I +L +IA Y V F+ ++F +++ D++F++ S++ + F+++ F++ + + + RV + +++ Y + K+ D + ++IE + T GKA++ + S R+ + ++I DG+ +D E E A L++ G+ VF G +E++L +AS P + +F V +F+ L + LC ++I+ + C T++ D+ F +D S SI P ++ ++F+ ++ I + ++GVV + EFGL+ + + + +AV+ I G T+ G AL ++ +S +VP+ +V++TDG S+
Sbjct: 15 CTQEVKADIVFLVDGSWSIGRPNFQQVRHFLSTLVSSFDVSPDHVRIGLAQYSDDSRTEFLLRTFQNNRDVLQYIARLPYKGGGTMTGKGL----DFLLQEHFVEAAGSRASSGVTQIAVVITDGKSQ--DEVKSQAEKLKQRGIILYAIGIKNAIL-TQLKEIADEQNIYTVSDFSAPSQGTDRFLFVVSGSDSYLFFSAECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVE--EPAQQLREQGVIVF-----GIANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSS 534 HSP 6 Score: 91.6633 bits (226), Expect = 6.243e-18 Identity = 96/386 (24.87%), Postives = 185/386 (47.93%), Query Frame = 3
Query: 957 IEISTPDQNCNN-QQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK--GREGAK--KTVILVTDG--ADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--------TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD--ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDF 2039
+++ P ++C ++ D+IF++D S+S++L + F +++ + ++ GVL+YS+ +M L + + E+ K +S L+ G T+ AL+ + F ++ GR + + ++++TDG D + ++P +E L+ G+ + IG+ + + QL M+ +D SK+ D +I+++ C KP +C + D+ +VD S +Q ++ F ++ F +G R L F++ E +D + + + + GG T I AL + R ++ S+ S+R +VL+T+G S + V+ A DL+ G++I IG+GD D E L+I T F Q+F
Sbjct: 779 LQLCDPQRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEP---SEALRSKGVNIFSIGVKGANMT-QLEIMAG-------QDKSKVFFVDNFAALEGLYKNISKELCNSTKP--VCEKQQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGGGTNIGLALK-SIREYFETSRGSRRSEGISQNLVLITDGESQDDVE--DPADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNF 1148 HSP 7 Score: 68.5514 bits (166), Expect = 6.068e-11 Identity = 51/180 (28.33%), Postives = 88/180 (48.89%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALN--STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
DI ++D S SI +F+ + I +F+ F I P R+ + Y D + EF + + L AVR ++ GG + GRAL +L+D + R ++ L++++DG ++DE VK A K++ KGVTIYT+ + L K + +F + + +++ IC
Sbjct: 420 DIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTET-GRALTFMGSLFDKAKDSR-GHKVPEYLVVITDGKSSDE--VKSPAEKLRSKGVTIYTIGVKEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDIC 595 HSP 8 Score: 53.9138 bits (128), Expect = 1.846e-6 Identity = 39/142 (27.46%), Postives = 73/142 (51.41%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAV-RLHRKVGGDSSVLGRALNSTLYDIVPE---MRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVA 563
DI ++D SSSI F+ + + + +S I P+ RV Y D EFL H + +L A+ RL + G + G+A++ L E R + +I ++++DG ++D+ V++ A +++E+GV ++ +A
Sbjct: 217 DIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTET--GKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDD--VEEPAQQLREQGVIVFGIA 354 HSP 9 Score: 51.6026 bits (122), Expect = 8.341e-6 Identity = 41/143 (28.67%), Postives = 73/143 (51.05%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLF-TKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEM---RPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAI 566
DI ++D S SI FQ ++ +S F + P++ R+ Y D +TEFL T + L + RL K ++ G+ L+ L + E R +S + +I ++++DG + DE VK A K++++G+ +Y + I
Sbjct: 22 DIVFLVDGSWSIGRPNFQQVRHFLSTLVSSFDVSPDHVRIGLAQYSDDSRTEFLLRTFQNNRDVLQYIARLPYKG--GGTMTGKGLDFLLQEHFVEAAGSRASSGVTQIAVVITDGKSQDE--VKSQAEKLKQRGIILYAIGI 160
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: COL6A6 (collagen type VI alpha 6 chain [Source:HGNC Symbol;Acc:HGNC:27023]) HSP 1 Score: 224.942 bits (572), Expect = 7.126e-59 Identity = 281/1137 (24.71%), Postives = 512/1137 (45.03%), Query Frame = 3
Query: 924 FETKRTTTSNYIE---ISTPDQNCN-NQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEI----QKFVSKLELLEGATFISNALRRSGEEFI---SKGREG-AKKTVILVTDGADNSPTMKPEVEAEKL-KKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFAN--EAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFP---NPFLRQDFXXXXXXXXXXXXXXCKT-----------TPAIKPQTC--SSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF--KIDYRAD-VAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFK-EDYKNFES---SIEDMTTSTNGVFIGKALTSARVLFKSDTRKNA----TKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKA--LFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL---RECKNELLNSTRKE-VPKVIVLVTDGVS 4163
++ +T TS+ ++ +S DQ ++ IFVL S + Q N + L + +++G+ Y ++++ L K EI ++F L + AL + F + GR + ++V G D+ ++ +A +L K GI V+ + LG S+ +++ +++ Y I + I Q A ++ DI ++D S + + +F + ++G N+ R +V + + EA+V ++L ++ L +VGG T+ ALN +S + K S+ ++ V++T+G S + V+ +AA L+ SGV + ++G+ DKA+ EL +++ P + F+ + F E L K KV C + AIK + C + DI F++D S + + + ++ +Q+ I R+ ++ Y+D EF L+ ++ A++ ++ G G + AL +F D R V + +V+ TD +++ S AE L+ GV I +G+ + E K + + + ++ K+++ IC A + C D++ ID S S+ P++++ DF+ ++ + ++ +G+ F+S + S F DM + IN + + G T G A+ + Y + GR + + LVV+TDG A D P A++ K G+KI ++GV + + L S Y+ + F L+ + ++ + C PQ+ D+IF++ S + + E FK M FM S+ T V + TR +L ++ N FK +K + +I + + + GKAL F ++ A + +++I DG+ TDRN E + L+ G+ +F+IG+ G + + L+ +A DK+ FVDNF+ L+ IS ELC+ T C +Q DL+F +D S SI + +F +V+ I +VG+ F++ + EF L+QF+E + + + G T+IG AL RE S R E + + +VL+TDG S
Sbjct: 187 YQALQTLTSSLMQTICVSMEDQRLALAERFADIFVLVDSGISQTEFQQVRLLLNRLTNQLNLGASTNRLGLAQYGKVVKVEFLLNKHNTKEEILDSLKRFRISRPLPSDPRNLGAALEFARANFFTVEAGGRAAVGSRQFLVVLSGKDSDDSV---YKASRLIKSAGITVVGMSLGASMT--EMSVVATPPYVYQIPVATAAPTLKGI-IGQQAAKVA----------DIVFVIDQSGSIGTANFQLVRMFVHSIVSGLDVGQNRVRVGVVVYNDKPEALVYLNTFKNKQNLLNFIQILPYVGGGTKTGAALNFTLQSVFTKESGSRTNTGVQQVAVVITDGESQDSVR--EAAASLRRSGVIVYSVGV-DKANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIK-EGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKS--PAEKLRSKGVTIYTIGVKEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSA---DGEVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQDEVRGPA-EALREK--GVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLES-LLALQLCD-PQRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYS--NDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEPSEALRSKGVNIFSIGVKG-ANMTQLEIMAGQDKSKVFFVDNFAALEGLYKNISKELCN---STKPVCEKQQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGGGTNIGLALKSIREYFETSRGSRRSEGISQNLVLITDGES 1287 HSP 2 Score: 196.438 bits (498), Expect = 5.124e-50 Identity = 261/1121 (23.28%), Postives = 517/1121 (46.12%), Query Frame = 3
Query: 1002 DLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNAL-----RRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD-QLNKMSSTETY-YPIEDMSKMALFDTTDIAQDACQIPKPKRICSAK--LDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEF--------THIYDDILKKVESLKHVGGFTR-IDKALNLASRSFYL------KSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQ-VFKIDYRA----DVAKIVVLFTDKLPTNQKDSLSEAEI-LKGDGVKIIFVGLSKNSPYNFYE--NLAXXXXXXXXXXXXXXXXXXXXXXXMIC-DAFKQR----------QDECTM-KKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGRED----VPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE-FYNVKTFTDLEKFNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGV-------FIGKALTSARVLFKS--DTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECS-TKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKN--ELLNSTRKE--VPKVIVLVTDGVST 4166
D++F++D SSS+ + +++ F V+ L I +VG+ YSD L + + + + +L G T A+ + EE S+ R+ + +++TDG + +P A++L++ G+ V IG+G + + + QL + + + IE+ + T+ + Q C + +R+ A+ DI ++VD S + E+ L R Q LG + R L + VV+ EF I D LK+ + + R + AL A +F+ + S++ +V+L+ S + V +A+ ++ +G+ + + LG A E+ +++T P++ Q V + T I Q + DI+FV+D+S + +R + + + L++ + R+ ++ Y+D+ L+ K+++ I+ L G G ALN Q VF + + V ++ V+ TD +DS+ EA L+ GV + VG+ K + + + K+ +++ ++ K +++ ++C + +Q ++ C ++ D+ ID S S+ P +F+ ++F+ +Q + R+G+ + L +++ + +++ + I G T G A+ LF K ++ VP+ LVV+TDG ++D +SP A K + G+ I ++GV ++ +KEL +I+ + + V F L K I++ C + +CK +GDL+F++ SS + + +++M DFMK + + + + V +L+F+ + D ++F S EDM + NG+ G ALT+ F S R + + ++++ DG+ D E A L++ G+K++ IG+ + L+ S D+ +F L+ + ++ +LCD ++C T++ D++F +D S+SI + +++ F+ +V + N T+ GV+ ++ D + F L+QF + ++ A+ + G T G+AL+ C N R E VP++++++TDG +T
Sbjct: 23 DIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVEEP---AQQLREQGVIVFGIGVGQANLGELQLIANRPAQRFLFTIENYQALQTL-TSSLMQTICVSMEDQRLALAERFADIFVLVD--SGISQTEFQQVRLLLNRLTNQLNLGASTNRLGLAQYGK--VVKVEFLLNKHNTKEEILDS-LKRFRISRPLPSDPRNLGAALEFARANFFTVEAGGRAAVGSRQFLVVLSGKDSDDSV--YKASRLIKSAGITVVGMSLG--ASMTEMSVVAT--PPYVYQ----------IPVATAAPTLKGIIGQQAAKVADIVFVIDQSGSIGTANFQLVRMFVHSIVSGLDVGQNRVRVGVVVYNDKPEALVYLNTFKNKQNLLNFIQILPYVGGGTKTGAALNFTLQSVFTKESGSRTNTGVQQVAVVITDG---ESQDSVREAAASLRRSGVIVYSVGVDKANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRAL-TFMGSLFDKAKDSRGHKVPEYLVVITDGKSSDEVKSP---AEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITD-ICSADGE--VCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEF----DLRSFFSK-EDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQD--EVRGPAEALREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLESLLALQLCD----PQRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDAT 1096 HSP 3 Score: 195.667 bits (496), Expect = 8.327e-50 Identity = 242/1005 (24.08%), Postives = 454/1005 (45.17%), Query Frame = 3
Query: 1569 DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP---FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSN----GNGGDLNTALNRARQVF---KIDYRADVA--KIVVLFTDKLPTNQKDSLSEA-EILKGDGVKIIFVGLSKN---------SPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSE----VYLNVPIDSNFEN-ADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK-----GREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSG---EFYNVKTFTDLEKFNAK-------TISNEACQIPQKPTICKAA------GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKE--DYKNFESSIEDMTTSTNGVFIGKALTSARVLF--KSDTRKN-ATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNS--TRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFI-----TRESCKAGKKA 4376
DI +VD SS + + + LF + + ++G +K R L +++E E +D +L +E L + G T KA++ ++ + S+ ++ V++T+G S + V+ + A L+E GV + IG+G +A+ EL++++ P F ++++ ++L ++ C + + DI +VD T + +R L+ LN+ +T+R+ + Y EF L++ TK +I ++K+ R + + +L AL AR F + RA V + +V+ + K + DS+ +A ++K G+ ++ + L + PY + +A+ K + K D++ ID+S S+G NFQ+ FV+ IV LD+ ++R R+G+ +N + VYLN F+N ++ I +P++ G T G A+ +FTK V +V VV+TDG + +S + +A + G+ + SVGV + +E EL IAS + VK+F +L K I +A + + + K D+ F++ S + FK M +F+ + + + R+ ++K+ ++ G + D +N E+++ + G G+ALT LF D+R + + +++I DG+ +D E A L+ G+ ++TIG+ + + +L+ ++ P + FV++F L + +++C + C DL+F +DSS SI P ++ DF++ ++ I N VGV+ F++ + EF L F K M+ A+ + G T G+AL ++ R + + +V+VTDG + + E+ +++ G+KI IG+ D+ + L+I S Y DF LKD ++ + CK ++A
Sbjct: 23 DIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVE--EPAQQLREQGVIVFGIGVG-QANLGELQLIANRPAQRFLFTIENYQALQTLTSSLMQTICVSMEDQRLALAERFADIFVLVDSGISQTE--FQQVRLLLNRLTNQLNLGASTNRLGLAQYGKVVKVEFLLNKHNTKEEILDSLKRFRISRPLPSDPRNLGAALEFARANFFTVEAGGRAAVGSRQFLVVLSGK---DSDDSVYKASRLIKSAGITVVGMSLGASMTEMSVVATPPYVYQIPVATA-----------------------APTLKGIIGQQAAKVADIVFVIDQSGSIGTANFQLVRMFVHSIVSGLDVGQNRVRVGVVVYNDKPEALVYLN-----TFKNKQNLLNFIQILPYVGGGTKTGAALNFTLQSVFTKESGSRTNTGVQQVAVVITDGESQ--DSVREAAASLRRSGVIVYSVGV-DKANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMGSLFDKAKDSRGHKVPEYLVVITDGKSSD--EVKSPAEKLRSKGVTIYTIGVK-EADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDICSA---DGEVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDGQAQDEVRGPAEA--LREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLESLLALQLCDPQRDCKKTERA 980 HSP 4 Score: 185.652 bits (470), Expect = 1.135e-46 Identity = 232/979 (23.70%), Postives = 442/979 (45.15%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQEL-AFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRS-----GEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD----QLNKMSSTETYYPIEDMSKMALFDTT-------DIAQDACQIP------KPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSK-----RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNP-FLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSS-NGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVF--KIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECT-MKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTK--GRED--VPKVLVVLTDG----ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN---RGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELC 3785
D++FV+D S S+ Q + F ++++ L + +VGV++Y+D E + L + +NK + F+ L + G T AL + +E S+ G ++ +++TDG + A L++ G VIV +G+ + Q+ E + ++ +++ +I + A + K + + + DI ++D S + ++ + + F FI+ F +G R +V +A+ +E D ++ V +++ GG T +AL S + K+K+S+ +V++T+G S + V++ A L+ GV I TIG+ +AD++EL+ +S P F DF + +K + C + + C +GD++F++D S + ++ ++ M + I R + ++ +S EF L F+K D+ AI ++ G G AL Q F + R + + +V+ TD Q + AE L+ GVKI +G+ + E S + + + + + +CD Q +C ++ D+I +D STS+ ENF+ F+ +V + ++ TR G+ ++++ + ++ ++ K I+ + G+T G A+ + + GR + VP++L+V+TDG N++ E + A+++K G+ I S+GV G +++ E+ + + V F LE K IS E C KP K DL+F++ S + + + M +F L K + ++ RV + +F+ + + + F ED + E +E MT + G IG AL S R F+ S + ++ +++I DGE D E + A+ L+ GI++F IG+ L+ +P + V NF +L+ + +C
Sbjct: 399 DIVFVIDQSGSIGTANFQLVRMFVHSIVSGLDVGQNRVRVGVVVYNDKPEALVYLNTFKNKQNLLNFIQILPYVGGGTKTGAALNFTLQSVFTKESGSRTNTGVQQVAVVITDGESQDSVREA---AASLRRSG--VIVYSVGVDKANETELIQIASHPPKEHVFIVKSFAELTPLKVKLQKVLCYNIIRQAVTVAGRSSAIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTETGRALTFMG-SLFDKAKDSRGHKVPEYLVVITDGKSSDEVKS--PAEKLRSKGVTIYTIGV-KEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDIC----SADGEVCKDLSGDLVFLIDSSGSIYPQDYEKMKDFMKFVISRSFIGRNEVHVGVLQFSSSPQPEFDLRSFFSKEDMLQAINGMQQLGGGTLTGDALTAVSQYFDSALGGRPHLQQRLVVVTDG--QAQDEVRGPAEALREKGVKIYAIGVVDANTTQLLEISGSHDRMYSGRDFDALKDLESLLALQLCDP----QRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEPSE--ALRSK--GVNIFSIGVKGANMTQLEIMAGQDKSKVFFVDNFAALEGL-YKNISKELCN-STKPVCEKQQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILE-MTQTGGGTNIGLALKSIREYFETSRGSRRSEGISQNLVLITDGESQDDVE--DPADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNFGSLENIKQKVVDTIC 1349 HSP 5 Score: 126.716 bits (317), Expect = 9.754e-29 Identity = 121/523 (23.14%), Postives = 240/523 (45.89%), Query Frame = 3
Query: 2679 ECTMKKL-DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFT-----KGREDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQ-QISEKELNDIASSGEF-YNVKTFTDLEKFNAKTISNEACQIP-QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIED--------MTTSTNGVFIGKALTSARVLF---KSDTRKN-ATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELL-----NSTRKEVPKVIVLVTDGVS 4163
EC M + D++ +D S+S+G +NF+ F+ +V LD+ D+ R+G+ ++ E Y + +F+ + + I ++P+ G T G AI FT + R+ VP++ VV+TDG ++D+ E Q + + G+ + +GVGQ + E +L + F + ++ + L+ + + + Q+ + + D+ ++ S ++T F+Q+ + LT + R+ + ++ G + F + N + I D ++ +G AL AR F ++ R ++ ++++ G+ +D + ++ + L+K GI V +G++ S +++ VA+P Y +++ +KG + + K D++F +D S SIG +Q FV IV +D+ N +VGVV +N L+ F K +++ ++ + + G T G AL + + T V +V V++TDG S
Sbjct: 15 ECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDDVEEPAQ----QLREQGVIVFGIGVGQANLGELQLIANRPAQRFLFTIENYQALQTLTSSLMQTICVSMEDQRLALAERFADIFVLVDSGISQT--EFQQVRLLLNRLTNQLNLGASTNRLGLAQY--GKVVKVEFLLNKHNTKEEILDSLKRFRISRPLPSDPRNLGAALEFARANFFTVEAGGRAAVGSRQFLVVLSGKDSD-DSVYKASRLIKSAGITV--VGMSLGASMTEMSVVATPPYV-----------YQIPVATA-APTLKGIIGQQAAKVADIVFVIDQSGSIGTANFQLVRMFVHSIVSGLDVGQNRVRVGVVVYNDKPEALVYLNTFKNKQNLLNFIQILPYVGGGTKTGAALNFTLQSVFTKESGSRTNTGVQQVAVVITDGES 514 HSP 6 Score: 102.064 bits (253), Expect = 3.605e-21 Identity = 108/426 (25.35%), Postives = 205/426 (48.12%), Query Frame = 3
Query: 957 IEISTPDQNCNN-QQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISK--GREGAK--KTVILVTDG--ADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT--------TDIAQDACQIPKPKRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDD--ILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL------VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKST 2159
+++ P ++C ++ D+IF++D S+S++L + F +++ + ++ GVL+YS+ +M L + + E+ K +S L+ G T+ AL+ + F ++ GR + + ++++TDG D + ++P +E L+ G+ + IG+ + + QL M+ +D SK+ D +I+++ C KP +C + D+ +VD S +Q ++ F ++ F +G R L F++ E +D + + + + GG T I AL + R ++ S+ S+R +VL+T+G S + V+ A DL+ G++I IG+GD D E L+I T F Q+F +++ +K V CK+ P+ P +C+ DI D ST
Sbjct: 965 LQLCDPQRDCKKTERADIIFLVDGSTSISLENFKSMQKFMLSMVNQTTVGENLTRFGVLLYSNDPKMMFKLNQFKTRQEVLKAISGLKPPSGDTYTGKALKFCLQFFSAENGGRAELQVPQILMVITDGDATDRNNLVEP---SEALRSKGVNIFSIGVKGANMT-QLEIMAG-------QDKSKVFFVDNFAALEGLYKNISKELCNSTKP--VCEKQQADLVFLVDQSGSIQTGDYDLMKNFTTELVKSFSIGQKLVRVGLAQFSSSFQHEFYLNQFSEDTEVSEHILEMTQTGGGTNIGLALK-SIREYFETSRGSRRSEGISQNLVLITDGESQDDVE--DPADDLRALGIEIFAIGIGDVHDLELLQITGTPQRLFTVQNFGSLENIKQKVVDTICKSKPSGDPSSCTI--DIAMGFDIST 1372 HSP 7 Score: 68.1662 bits (165), Expect = 7.543e-11 Identity = 53/192 (27.60%), Postives = 94/192 (48.96%), Query Frame = 3
Query: 117 ALDQRCIG-KPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALN--STLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVIC 683
A+ + C+ + DI ++D S SI +F+ + I +F+ F I P R+ + Y D + EF + + L AVR ++ GG + GRAL +L+D + R ++ L++++DG ++DE VK A K++ KGVTIYT+ + L K + +F + + +++ IC
Sbjct: 594 AIKEGCLQTEEADIFFLIDHSRSIYPSDFKDMINFIIEFIQTFRIGPQYVRMGVVKYADLPELEFGLETYMDAENLETAVRTIQQRGGGTET-GRALTFMGSLFDKAKDSR-GHKVPEYLVVITDGKSSDE--VKSPAEKLRSKGVTIYTIGVKEADKKELEEISGDPKRMFFVNDFDALNPIKDKVITDIC 781 HSP 8 Score: 63.1586 bits (152), Expect = 2.520e-9 Identity = 35/125 (28.00%), Postives = 72/125 (57.60%), Query Frame = 3
Query: 3810 ECSTKQL-DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEAL-----RECKNELLNSTRKEVPKVIVLVTDGVST 4166
EC+ + D++F +D SSSIG D ++ F+ +V +DI + +VG+ ++ + +EF L + ++++A++R+ + G T+ G+A+ + E + R+ VP++ V++TDG S+
Sbjct: 15 ECAMATVADIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTETGKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSS 139 HSP 9 Score: 57.7658 bits (138), Expect = 1.159e-7 Identity = 47/185 (25.41%), Postives = 90/185 (48.65%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAV-RLHRKVGGDSSVLGRALNSTLYDIVPE---MRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMF--PEFQTMRGDSENLVSVICV 686
DI ++D SSSI F+ + + + +S I P+ RV Y D EFL H + +L A+ RL + G + G+A++ L E R + +I ++++DG ++D+ V++ A +++E+GV ++ + +G L L + +F +Q ++ + +L+ ICV
Sbjct: 23 DIVFLVDGSSSIGIDNFEEVRLFLREVVSGLDIGPDKVRVGLAQYSDEPYQEFLLKDHFDASSLLAAIERLPYRTGQTET--GKAISFLLSQYFTEEAGSRARQRVPQIAVVITDGDSSDD--VEEPAQQLREQGVIVFGIGVGQANLGELQLIANRPAQRFLFTIENYQALQTLTSSLMQTICV 203
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: COL6A3 (collagen type VI alpha 3 chain [Source:NCBI gene;Acc:101161016]) HSP 1 Score: 120.168 bits (300), Expect = 1.202e-26 Identity = 232/1042 (22.26%), Postives = 455/1042 (43.67%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGA--------TFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKD-QLNKMSSTETY-YPIEDMSKMA---------LFDTTD-----IAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE---EEFTHIYDDILKKVESLKHVGGFTR-IDKALNLASRSFYLKSKNSK------RLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPF-LRQDFXXXXXXXXXXXXXXCKTT--------------PAIKPQTCSSN-GDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRA-----RQVFKIDYRADVAKIVVLFTDK-LPTNQKDSLSEAEI-LKGDGVKIIFVGLS----------KNSPYNFY--ENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYL----FTKGREDVPKV----LVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFY-------NVKTFTDLEKFNAKTISNEACQIPQKPTICKAA---GDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE---SSIEDMTTSTNG-VFIGKALTSARVLFKSDTR-----KNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALF-VDNFSNLQRYNAYISSELCDVIKGTNKECSTKQL 3830
D++F++D S+ + + F + V++SL + K ++GV YSD + + L K + + +++ L G F+ + R E S+ EG + +I+V+ G+ + + LK+ RV+ + +G I QL +SS T+ + ++D+ + L D +D +A+D I P + K D+ ++D +++ + F + +E+ ++G+NK R S+V +++E +E EF+ + + +++ L GG R +AL SR+ + +S S+ + +++L +G S + V A+ L+ G+ IG D AD EL+ +S F + +D+++ K K T+ P I P + D++F++D +T +T + IR LM ++ L+I +R+S++ YSDE+ EF L+E TK ++ I +L S G G NT RA R +++ + + + V F L D +S + LK G+ + +G + ++P FY N + + + I + + D + K D+I ID S S GP F DF+ +IVQ L+++ D+ R+G+ + V + ++S+ A + I ++ I G ++ + +A DY+ F P V LV++T G D+ + Q+ L +I S+ +G + +L IAS+ + + N++T L++F A+ +P T + D++F++ S N F+ + F+ S+ D+ + ++++ ++ + D+ F Y N E ++IE + + G A+ + + + R + + ++++ G D + T A L++ G++V+ +G +++L+ +AS + NF + + L + +KG K C T+ L
Sbjct: 383 DIVFLVDGSNGVGREFPIIQDFISKVVESLDVGEKRIRIGVAQYSDFAQSYINLNDHATKQSVLNGIREIKRLGGTQRNLGVALAFVRQNMLRP--EKGSREEEGVPQFLIIVSSGSSTDDFIG---QVTDLKRS--RVVTLNIGTRDISSPQLRIVSSLPTFAFTVDDLPGLYTVQAPLVNILTDLSDEYIKSLARDLAGI-IPALVGGIKKDVVFLIDGTTKAS-SNFPAIRNFMRSLVEKLDIGLNKARVSVVQYSDEPKLEFLLNEFS-TKNQVQERIMRLTSKGGSRRNTGRALEWVSRNIFQRSAGSRIEEGVPQFLIVLISGKSSDDVSRSSAS--LKRLGIAPVPIGSAD-ADSNELRQISFNQELFYVVKDYEQFAEEQSKFFKTITSTSTGDITRSQAFPSVFPGITPAPAGGDKKDVVFLIDGTT-STRSKFPSIRILMRILVEKLDIGLDKARVSVVQYSDESKLEFPLNEFSTKNEVQERIMRLTSKG-GTRRNTG--RALEWVSRNIYQRSAGSRIEEGVPQFLIVILGGKSSDDVSRSSASLKRLGIASVPIGSADADSNELKQISSNPEVFYIVNNFEQFARNQPTFMESLTSTSTGDITRAVAPPYVP-PDLLDIGKKDIIFLIDGSASTGPTGFAHVRDFLLKIVQQLNVQPDQVRVGVVQYADRVKTDFSLNSHNNKAAVINAIQRLRNIGGRSS---ELPDAIDYIMKNEFQPSAGARPTVATQHLVIITAGRSTKDVNQYGQL------LKSTQINSIAIG----DTQLIQIASTPKDFIPIVSLPNLQTQEVLQQFIARLAGLTPVTLPDDETPSGKSSRQADIVFLVDGSINLGRSNFEAVMQFINSMIDNLYDDESSLQISLAQYA-ADVTDAFFLNTYDNKENIVTAIEQVQYRGGPRINTGAAIQHIQNVHFTKERGSRMDQGVPQILMVVTGGASADDSRTASLA--LQRKGVRVYAVG--AGNIQTELEGIASESTTVARSSNFQAISDLVELLMPALTEELKG-QKLCVTESL 1387 HSP 2 Score: 79.7221 bits (195), Expect = 2.606e-14 Identity = 119/541 (22.00%), Postives = 236/541 (43.62%), Query Frame = 3
Query: 2691 KKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKE-----INKIPFIEG---NTAIGWAIGNATDYLFTKGR-----EDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASS----GEFYNVKTFTDLEKFNAKTISNE-----ACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFN--RGNYI---DSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFK---SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQRYNAYISSELCDV----IKGTNKECS--------TKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGA-TDIGEALRECKNELLNST-----RKEVPKVIVLVTDGVST 4166
K+ D++ +D S +LG NF+ + F+N I+ NL + +I + + ++V D+ F N KE I ++ + G NT G AI + + FTK R + VP++ +V+T GA+ + + +++ + G+++ +VG G + EL IAS N + +DL + ++ E C C D++F++ S N F + DF+ + + V ++ R+ + +++ +YI D K+ N I+ + + + + A +L S + + +II+ G TD + + LK+ + IG T S L+ V+S P A VD+ L A + + L D+ IK ++ + + D++F +D ++ ++ +F+ +V +DI N +V VV ++ + + EF L++F+ K + + R+ G+ + G AL + + + VP+ ++++ G S+
Sbjct: 180 KQADIVFLVDGSINLGRSNFEAAMQFINGIIDNLYDDESSLQISLAQYAADV-----TDAFFLNTYDNKENIVTAIEQVQYRGGPRINT--GAAIQHIQNVHFTKERGSRMDQGVPQIFMVVTGGAS--ADDSRTASLALQRKGVRVYAVGAGN--IQTELEGIASESTTVARSSNFQAISDLVEQLMPALTEELKGQKLCVTESLTPRCYPRKDIVFLVDGS-NGVGREFPIIQDFISKVVESLDVGEKRIRIGVAQYSDFAQSYINLNDHATKQSVLNGIREIKRLGGTQRNLGVALAFVRQNMLRPEKGSREEEGVPQFLIIVSSGSSTD--DFIGQVTDLKRSRVVTLNIG-TRDISSPQLRIVSSLPTFAFTVDDLPGLYTVQAPLVNILTDLSDEYIKSLARDLAGIIPALVGGIKKDVVFLIDGTTKASSNFPAIR-NFMRSLVEKLDIGLNKARVSVVQYSDEPKLEFLLNEFSTKNQVQERIMRLTSKGGSRRNTGRALEWVSRNIFQRSAGSRIEEGVPQFLIVLISGKSS 704 HSP 3 Score: 77.7962 bits (190), Expect = 9.938e-14 Identity = 112/494 (22.67%), Postives = 219/494 (44.33%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTA-IGWAIGNATDYLFTKG-----REDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEK-ELNDIASSGE-FYNVKTFTDL----EKFNAKTISNEACQIPQK-------PTICKA-AG----DLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVF---IGKALT-SARVLFK----SDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDKALFVDNFSNLQR-----YNAYISSELCDVIKGTNKECSTKQL------DLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRI 4043
K DV+ ID +T NF +F+ +V+ LD+ ++ R+ + ++ E L ++ +Q+ I ++ G+ G A+ + +F + E VP+ L+VL G + S +S A L + I V +G ++ EL I+ + E FY VK + KF S I + P I A AG D++F++ + + T +F + M+ L + + + RV++++++ + ++ E E M ++ G G+AL +R +++ S + + +I+I+ G+ +D + + LK+ GI IG + ++LK ++S P+ V+NF R + S+ D+ + L D++F +D S+S GP + DF+ +IV+ +++ + +VGVV + + +F L+ K A+I+A++R+
Sbjct: 586 KKDVVFLIDGTTKAS-SNFPAIRNFMRSLVEKLDIGLNKARVSVVQYSDEPKLEFLLNEFSTKNQVQERIMRLTSKGGSRRNTGRALEWVSRNIFQRSAGSRIEEGVPQFLIVLISGKS----SDDVSRSSASLKRLGIAPVPIGSADADSNELRQISFNQELFYVVKDYEQFAEEQSKFFKTITSTSTGDITRSQAFPSVFPGITPAPAGGDKKDVVFLIDGTTS-TRSKFPSIRILMRILVEKLDIGLDKARVSVVQYSDESKLEFPLNEFSTKNEVQERIMRLTSKGGTRRNTGRALEWVSRNIYQRSAGSRIEEGVPQFLIVILGGKSSD--DVSRSSASLKRLGIASVPIG-SADADSNELKQISSNPEVFYIVNNFEQFARNQPTFMESLTSTSTGDITRAVAPPYVPPDLLDIGKKDIIFLIDGSASTGPTGFAHVRDFLLKIVQQLNVQPDQVRVGVVQYADRVKTDFSLNSHNNKAAVINAIQRL 1070
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: si:ch1073-291c23.1 (vitrin [Source:NCBI gene;Acc:101168147]) HSP 1 Score: 108.997 bits (271), Expect = 1.506e-23 Identity = 81/335 (24.18%), Postives = 159/335 (47.46%), Query Frame = 3
Query: 3231 IPQKPTI-------CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFK--EDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP---DKA---------LFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
+P+ P + CK DL F+M S + RFK DF+ + ++ V + I+++ + + + ++++E + +G+AL+ + SD R A ++++DG TD+ E E + L ++ GI +F + + G ES+ +++ DKA L V+++ L++ + +CD + + D+ F +D SSS+G ++T L+FV + R+ +I+ T+VG V + + R EF Q+ K +++A+KRI + G T G A+ +L + ++ K+++++TDG S
Sbjct: 375 VPRAPEVVSQGDPNCKV--DLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGWPTDKVE--EASRLARESGINIFFVTIEG-PDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRS 704 HSP 2 Score: 81.2629 bits (199), Expect = 5.195e-15 Identity = 88/387 (22.74%), Postives = 179/387 (46.25%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKE--LND------IASSGEFYN--VKTFTDLEKFNAKTISNEACQIPQ--KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDK--ALFVDNFSNLQRYNAYISSELC 3785
K+D+ +D S S+G F+I+ DF+ + Q +++ +GI + E + + + + +++ + KI G + +G A+ F+ R P V VVL DG + + E+ ++ A+ GI I V + G SE++ + D + + F++ V ++ L K + + C + C A D+ FV+ S++ + F+ + +F+ ++T+ + +TRV +++ ++ F + E ++I+ + + G G A+T A S ++ N K +I+I DG D + A + + G+ ++IG+ ++ +L+ +A+ PDK + FVD F NL ++ I +C
Sbjct: 390 KVDLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGWPTDKVEEASRL----ARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRK-AVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRSYD--DVRAPALAVHRQGVIAYSIGIAW-AAQDELEYIATDPDKEHSFFVDEFDNLYKFVPKIIHNIC 768 HSP 3 Score: 80.8777 bits (198), Expect = 7.245e-15 Identity = 90/386 (23.32%), Postives = 170/386 (44.04%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFV---GLSKNSPYNFYEN--------LAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE----FYNVKTFTDLEKFNAKTISNEACQ 3230
D+ F++D S + + + Q +N+ + II Y DE TE L + R++ A++++ G + AL+ + + D R + V+ D PT++ + S + + G+ I FV G ++ N E+ + S+ + + + K + + +CD + + + D+ ID S+S+G NF+ L+FV + + ++ TR+G + E L A++ I +I + G T+ G AI A + LF+K + + K+++V+TDG + D +P ++ + G+ S+G+ ++ EL IA+ + F+ V F +L KF K I N CQ
Sbjct: 392 DLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGWPTDKVEEASR--LARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRSYDDVRAPALAVHRQ---GVIAYSIGIAWA-AQDELEYIATDPDKEHSFF-VDEFDNLYKFVPKIIHN-ICQ 769 HSP 4 Score: 75.485 bits (184), Expect = 3.301e-13 Identity = 85/379 (22.43%), Postives = 165/379 (43.54%), Query Frame = 3
Query: 963 ISTPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGL-------GLSVIKDQL--NKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-----------DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN 2018
+S D NC ++DL F++D S S+ + + F V + + + + +G++ Y D + L + + E++ V K+ G + + AL +++ S R GA +++ DG PT K E + ++ GI + + + ++++D + T ++ + S AL + A Q P KR+C DIA ++D SS V + L F +FE+ TR V + E +E F ++L ++ + + G T A+ A+ + KSK N +++++++T+G S + V+ A + GV +IG+ A ++EL+ ++T P+
Sbjct: 382 VSQGDPNC---KVDLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGW---PTDKVEEASRLARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFAL-------RKAVQ-PLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRSYDDVRA--PALAVHRQGVIAYSIGIA-WAAQDELEYIATDPD 743 HSP 5 Score: 53.5286 bits (127), Expect = 1.916e-6 Identity = 38/141 (26.95%), Postives = 70/141 (49.65%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTS-SPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
DIA ++D SSS+ G F++ L + F I +TRV + Y + EF F ++ + LN R++ GG S+ G A+ + + +P +I+++++DG + D++ LA + +GV Y++ I
Sbjct: 594 DIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTST--GAAITYAAEKLFSKSKPNKR--KIMIVITDGRSYDDVRAPALA--VHRQGVIAYSIGIA 728
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Match: si:ch1073-291c23.1 (vitrin [Source:NCBI gene;Acc:101168147]) HSP 1 Score: 108.997 bits (271), Expect = 1.592e-23 Identity = 81/335 (24.18%), Postives = 159/335 (47.46%), Query Frame = 3
Query: 3231 IPQKPTI-------CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFK--EDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDT---RKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASP---DKA---------LFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
+P+ P + CK DL F+M S + RFK DF+ + ++ V + I+++ + + + ++++E + +G+AL+ + SD R A ++++DG TD+ E E + L ++ GI +F + + G ES+ +++ DKA L V+++ L++ + +CD + + D+ F +D SSS+G ++T L+FV + R+ +I+ T+VG V + + R EF Q+ K +++A+KRI + G T G A+ +L + ++ K+++++TDG S
Sbjct: 377 VPRAPEVVSQGDPNCKV--DLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGWPTDKVE--EASRLARESGINIFFVTIEG-PDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRS 706 HSP 2 Score: 82.0333 bits (201), Expect = 3.312e-15 Identity = 88/387 (22.74%), Postives = 179/387 (46.25%), Query Frame = 3
Query: 2694 KLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKG---REDVPKVLVVLTDG--ANDIGESPQISAVKAKLGGIKIISVGV-GQQISEKE--LND------IASSGEFYN--VKTFTDLEKFNAKTISNEACQIPQ--KPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFE--SSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVAS-PDK--ALFVDNFSNLQRYNAYISSELC 3785
K+D+ +D S S+G F+I+ DF+ + Q +++ +GI + E + + + + +++ + KI G + +G A+ F+ R P V VVL DG + + E+ ++ A+ GI I V + G SE++ + D + + F++ V ++ L K + + C + C A D+ FV+ S++ + F+ + +F+ ++T+ + +TRV +++ ++ F + E ++I+ + + G G A+T A S ++ N K +I+I DG D + A + + G+ ++IG+ ++ +L+ +A+ PDK + FVD F NL ++ I +C
Sbjct: 392 KVDLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGWPTDKVEEASRL----ARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRK-AVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRSYD--DVRAPALAVHRQGVIAYSIGIAW-AAQDELEYIATDPDKEHSFFVDEFDNLYKFVPKIIHNIC 770 HSP 3 Score: 80.8777 bits (198), Expect = 6.388e-15 Identity = 89/385 (23.12%), Postives = 169/385 (43.90%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDY---RADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFV---GLSKNSPYNFYEN--------LAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDG-ANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGE---FYNVKTFTDLEKFNAKTISNEACQ 3230
D+ F++D S + + + Q +N+ + II Y DE TE L + R++ A++++ G + AL+ + + D R + V+ D PT++ + S + + G+ I FV G ++ N E+ + S+ + + + K + + +CD + + + D+ ID S+S+G NF+ L+FV + + ++ TR+G + E L A++ I +I + G T+ G AI A + LF+K + + K+++V+TDG + D +P ++ + G+ S+G+ ++ EL IA+ + + V F +L KF K I N CQ
Sbjct: 394 DLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGWPTDKVEEASR--LARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFALRKAVQPLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRSYDDVRAPALAVHRQ---GVIAYSIGIAWA-AQDELEYIATDPDKEHSFFVDEFDNLYKFVPKIIHN-ICQ 771 HSP 4 Score: 75.8702 bits (185), Expect = 2.413e-13 Identity = 85/379 (22.43%), Postives = 165/379 (43.54%), Query Frame = 3
Query: 963 ISTPDQNCNNQQLDLIFVLDASSSLA-LYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKG---REGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGL-------GLSVIKDQL--NKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKL-----------DIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYD--DILKKVESLKHVGGFTRIDKALNLASRSFYLKSK-NSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPN 2018
+S D NC ++DL F++D S S+ + + F V + + + + +G++ Y D + L + + E++ V K+ G + + AL +++ S R GA +++ DG PT K E + ++ GI + + + ++++D + T ++ + S AL + A Q P KR+C DIA ++D SS V + L F +FE+ TR V + E +E F ++L ++ + + G T A+ A+ + KSK N +++++++T+G S + V+ A + GV +IG+ A ++EL+ ++T P+
Sbjct: 384 VSQGDPNC---KVDLAFLMDGSWSIGKRRFKIQKDFLAEVAQVINVGVAGPMMGIIQYGDEPVTEISLRTYSSSREVKAAVEKIVQKGGLSHVGRALSYINKQYFSDANGNRGGAPNVAVVLVDGW---PTDKVEEASRLARESGINIFFVTIEGPDESERQNLVEDNFVDKAVCRTNNFFSLPVNSWFAL-------RKAVQ-PLVKRVCDTDRLVCSKTCLNANDIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTSTGAAITYAAEKLFSKSKPNKRKIMIVITDGRSYDDVRA--PALAVHRQGVIAYSIGIA-WAAQDELEYIATDPD 745 HSP 5 Score: 53.9138 bits (128), Expect = 1.538e-6 Identity = 38/141 (26.95%), Postives = 70/141 (49.65%), Query Frame = 3
Query: 150 DIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTS-SPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
DIA ++D SSS+ G F++ L + F I +TRV + Y + EF F ++ + LN R++ GG S+ G A+ + + +P +I+++++DG + D++ LA + +GV Y++ I
Sbjct: 596 DIAFVIDGSSSVGTGNFRTVLEFVANVTREFEISDTDTRVGAVQYTYEQRLEFAFGQYNHKAELLNAIKRINYWSGGTST--GAAITYAAEKLFSKSKPNKR--KIMIVITDGRSYDDVRAPALA--VHRQGVIAYSIGIA 730
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000046768.1 (SMESG000046768.1) HSP 1 Score: 2695.61 bits (6986), Expect = 0.000e+0 Identity = 1421/1440 (98.68%), Postives = 1427/1440 (99.10%), Query Frame = 3
Query: 57 MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGXXXXXXXXXXXXXKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVRNXXXXXXXXXXXXIIRTTIPDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIPFETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKKA 4376
MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGSNLNIPLLLSISSKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVRNTT + + T I T PDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIP ETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTD+AQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALN+ASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDI TIGLGDKADREELKILSTFPNPFLRQDFKEEKSLLEKSVKVSCKTTPAIKPQTCSSNGDI+FVVDKSTDNTNEIWDGIRSLMLTTLQSLNISRTTSRISIIDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLASKKSSIKIENINKIDKNKEQIKNMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISN+ACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTLIKKLGIKIIVIGIGDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKKA
Sbjct: 1 MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIGSNLNIPLLLSISSKKNVVMFPEFQTMRGDSENLVSVICVVKYTKPPSRETISIPDITSSSATVRNTTVTSKQTETTIIRTTI-PDKTTSTGKKTDIAYTSQSSSIQKTSVKQTTKSIPVETKRTTTSNYIEISTPDQNCNNQQLDLIFVLDASSSLALYWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDLAQDACQIPKPKRICSAKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNIASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIVTIGLGDKADREELKILSTFPNPFLRQDFKEEKSLLEKSVKVSCKTTPAIKPQTCSSNGDIVFVVDKSTDNTNEIWDGIRSLMLTTLQSLNISRTTSRISIIDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLASKKSSIKIENINKIDKNKEQIKNMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNDACQIPQKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTLIKKLGIKIIVIGIGDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKAGKKA 1439
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000043380.1 (SMESG000043380.1) HSP 1 Score: 223.016 bits (567), Expect = 2.303e-61 Identity = 173/573 (30.19%), Postives = 297/573 (51.83%), Query Frame = 3
Query: 2679 ECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGAND-IGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIPQKPTI--CKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVF-IGKALTSARV---LFKSDTRKNATKTVIIIIDGEITDRNETFEE----ANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITRESCKA 4364
+C+ ++++ + +D S+S+ P++F++ F+ ++ + + D +R G+ +F+ + L + ++ + +K I F+ G TA+G AI A + + R +PKV++++TDG N+ + Q+ ++ K G+ II +G+G +IS +EL +IAS G +Y + T+ L KT+ C+ + I C+ GD+ FV+ S KQ+ L +L + + + RVAI+ N G + F S E G F +G ++S V ++ R + K V+++ T ++E FE A L+ G V T+ + S L +++ K + +F+ + I+ +C +K EC K+LD++F LD SSSIGP+ W +L FV RIV+ MD+ + T+VG V FN++A F LD++ K +I A++ + F +G T IG+AL +++ S R V++LVTDG S G PE E+ I+ G+ II IGI + S QL +S+S +Y I DF +L +T+ESC+A
Sbjct: 30 DCSKNRIEMAIVLDASSSIEPDHFKLAKQFIVDVIGDFVVGPDHSRFGMISFSDDAELLFTLTTHKTIEETKKAILDAKFLTGATAMGKAIQLAGTGILQESRTGIPKVILLITDGKNNKFPNAAQMGKLQ-KEAGVTIIGIGIGSEISPQELTEIASPGLYYQLDTYPSLIGL-VKTLKTSFCKKLENIQIDTCETEGDISFVIESQQKAGDLHKKQIELITHVLNRLEISEGKQ-RVAIVVENEGILVP------LNKFSSRSEYTQYLKKGDFKVGSKISSGLVESGRLLAENRTDIPKLVVLMR----TQKSENFENDVKAALALRNQGANVLTVAIGLNVDVSQLNELSTTRKTVIFKDFNFQVSFVNEIAENICQNLKKVYVECIAKKLDVVFVLDCSSSIGPENWMNQLQFVSRIVQMMDVGKDSTRVGSVYFNSEAYVGFSLDKYGTKDEVIKAIRNLPFSEGGTAIGDALNLAHDQMKASLRNNTVPVMILVTDGQSNMGRKPESEALEIRNDGVHIICIGITNEISKDQLIRISTSNRYYHINDFDSLNSVSVEAMTKESCQA 589 HSP 2 Score: 179.489 bits (454), Expect = 9.074e-47 Identity = 155/572 (27.10%), Postives = 289/572 (50.52%), Query Frame = 3
Query: 1554 CSA-KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFT----HIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNS-KRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQD-FXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQ-IPQKP 3245
CS ++++A+++D SS ++ + F I F +G + +RF ++SF+++A E FT ++ K + K + G T + KA+ LA +S+ ++++L+T+G + + Q +E+GV I IG+G + +EL +++ P + + D + L++ CK I+ TC + GDI FV++ S ++ L+ L L IS R++I+ ++ F+ R Y + G +++ L + ++ + R D+ K+VVL + N ++ + A L+ G ++ V + N + L++ + ++ ++ N +I IC K+ EC KKLDV+ +D S+S+GPEN+ +L FV+RIVQ +D+ KD TR+G FNSE Y+ +D ++ K I +PF EG TAIG A+ A D + R + V++++TDG +++G P+ A++ + G+ II +G+ +IS+ +L I++S +Y++ F L + + ++ E+CQ + KP
Sbjct: 31 CSKNRIEMAIVLDASSSIEPDHFKLAKQFIVDVIGDFVVGPDHSRFGMISFSDDA--ELLFTLTTHKTIEETKKAILDAKFLTGATAMGKAIQLAGTGILQESRTGIPKVILLITDGKNNKFPNAAQMGKLQKEAGVTIIGIGIGSEISPQELTEIAS-PGLYYQLDTYPSLIGLVKTLKTSFCKKLENIQIDTCETEGDISFVIE-SQQKAGDLHKKQIELITHVLNRLEISEGKQRVAIVVENEGILVPLN---KFSSRSEYTQYLKKGDFKVGSKISSGLVESGRLLA-ENRTDIPKLVVLMRTQKSENFENDVKAALALRNQGANVLTVAIGLNVDVSQLNELSTTRKTVIFKDFNFQVSFVNEIAENICQNLKKVYVECIAKKLDVVFVLDCSSSIGPENWMNQLQFVSRIVQMMDVGKDSTRVGSVYFNSEAYVGFSLDKYGTKDEVIKAIRNLPFSEGGTAIGDALNLAHDQMKASLRNNTVPVMILVTDGQSNMGRKPESEALEIRNDGVHIICIGITNEISKDQLIRISTSNRYYHINDFDSLNSVSVEAMTKESCQAVNNKP 594 HSP 3 Score: 116.316 bits (290), Expect = 3.467e-26 Identity = 132/543 (24.31%), Postives = 254/543 (46.78%), Query Frame = 3
Query: 951 NYIEISTPDQNCNNQQLDLIFVLDASSSLAL-YWDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKK-MGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDM-SKMALFDTTDIAQDACQIPKPKRI--CSAKLDIALIVDCSSQV-QLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVE--EEFTHI--YDDILKKVESLKHVGGF---TRIDKALNLASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILSTFPNPFLRQDFXXXXXXXXXXXXXXCKTTPAIKPQTCSSNGDIIFVVDKSTDNTNEIWDGIRSLMLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLS 2540
N +E+ D C+ ++++ VLDASSS+ ++ F +VI + S+ G++ +SD E+ L + + E +K + + L GAT + A++ +G + + R G K ++L+TDG +N + KL+K G+ +I IG+G + +L +++S YY ++ S + L T + C+ + +I C + DI+ +++ + LH+ Q+ + + E+ K R ++V NE ++ +F+ Y LKK G F ++I L + R + +LVVL+ S ++AA L+ G ++ T+ +G D +L LST + +DF + S + + + C+ + + + D++FV+D S+ E W + +Q +++ + ++R+ + ++ E F LD+ TK ++ AI+ L + G + ALN A K R + +++L TD + SEA ++ DGV II +G++
Sbjct: 22 NVVEVDNYD--CSKNRIEMAIVLDASSSIEPDHFKLAKQFIVDVIGDFVVGPDHSRFGMISFSDDAELLFTLTTHKTIEETKKAILDAKFLTGATAMGKAIQLAGTGILQESRTGIPKVILLITDGKNNK--FPNAAQMGKLQKEAGVTIIGIGIGSEISPQELTEIASPGLYYQLDTYPSLIGLVKT--LKTSFCKKLENIQIDTCETEGDISFVIESQQKAGDLHK--KQIELITHVLNRLEISEGKQRVAIV-VENEGILVPLNKFSSRSEYTQYLKK-------GDFKVGSKISSGLVESGRLLAENRTDIPKLVVLMRTQKSENFENDVKAALALRNQGANVLTVAIGLNVDVSQLNELSTTRKTVIFKDFNFQVSFVNEIAENICQNLKKVYVECIAKKLDVVFVLDCSSSIGPENWMNQLQFVSRIVQMMDVGKDSTRVGSVYFNSEAYVGFSLDKYGTKDEVIKAIRNLPFSEGGTAIGDALNLAHDQMKASLRNNTVPVMILVTDGQSNMGRKPESEALEIRNDGVHIICIGIT 548 HSP 4 Score: 94.3597 bits (233), Expect = 2.987e-19 Identity = 58/194 (29.90%), Postives = 101/194 (52.06%), Query Frame = 3
Query: 984 CNNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQI--PKPKRIC 1556
C ++LD++FVLD SSS+ W +L F + +++ + + ++VG + ++ + L K E+ K + L EG T I +AL + ++ + R +ILVTDG N KPE EA +++ G+ +I IG+ + KDQL ++S++ YY I D + + +++CQ KPK+ C
Sbjct: 406 CIAKKLDVVFVLDCSSSIGPENWMNQLQFVSRIVQMMDVGKDSTRVGSVYFNSEAYVGFSLDKYGTKDEVIKAIRNLPFSEGGTAIGDALNLAHDQMKASLRNNTVPVMILVTDGQSNMGR-KPESEALEIRNDGVHIICIGITNEISKDQLIRISTSNRYYHINDFDSLNSVSVEAMTKESCQAVNNKPKKSC 598 HSP 5 Score: 74.7146 bits (182), Expect = 3.839e-13 Identity = 55/182 (30.22%), Postives = 101/182 (55.49%), Query Frame = 3
Query: 3786 DVIKGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFD----DGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTL 4319
+V++ N +CS ++++ LD+SSSI PD+++ F+ ++ D + + ++ G+++F+ DA F L + I K+ I D GAT +G+A++ +L +R +PKVI+L+TDG + + L K+ G+ II IGIG SP +L ++S G +Y++ + +L
Sbjct: 22 NVVEVDNYDCSKNRIEMAIVLDASSSIEPDHFKLAKQFIVDVIGDFVVGPDHSRFGMISFSDDAELLFTLTT----HKTIEETKKAILDAKFLTGATAMGKAIQLAGTGILQESRTGIPKVILLITDGKNNKFPNAAQMGKLQKEAGVTIIGIGIGSEISPQELTEIASPGLYYQLDTYPSL 199
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000024982.1 (SMESG000024982.1) HSP 1 Score: 125.561 bits (314), Expect = 3.340e-30 Identity = 94/330 (28.48%), Postives = 171/330 (51.82%), Query Frame = 3
Query: 3195 FNAKTISNEACQIPQKPTICK-AAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENT--RVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTN---GVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVI-KGTNKECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVS 4163
FN KT ++ QI C D++F++ S + + + F+ F K + +T++ NT R+A +KF I F + Y + + D+T + N IGKAL RK K V++ DG + A+ LK+ +++ TIG+ + ++L +ASP +L V++F+ L + + + S +C + G K C+ +Q DL+ LDSS+SI + ++ +F+ + + + ++ +V +++F+ + R +FG + + MI+ VK +I GAT IG+ALRE ++L++ R VP I+++TDGV+
Sbjct: 17 FNVKT-DDQNDQITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDM--MTIIKNSNTENRIAFIKFGDDAEIVFEF-QTYTKLKEMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVN 342 HSP 2 Score: 123.635 bits (309), Expect = 1.779e-29 Identity = 94/364 (25.82%), Postives = 175/364 (48.08%), Query Frame = 3
Query: 2682 CTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEACQIP----QKPTICKAAGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYK--NFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQR 3755
CT DV+ +D S S+ ++F++ F ++ + RI F + + + + +M ++ + +T IG A+ ++ R++V K++V+ TDG N++ +P + A K K ++I+++G+G I+ EL +AS G V++FT L K ++ +S+ C IP +KP + DL+ ++ SS + + E ++Q +F+ + E RVA+L F+ D GF + YK + + ++ + IGKAL S R N ++II DG E AN LK G + ++G+ + + +LK ++S DK L V+ + +L++
Sbjct: 35 CTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKEMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSH-ICPIPDPGLEKPCTLEQQ-DLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKK 396 HSP 3 Score: 116.701 bits (291), Expect = 3.405e-27 Identity = 93/344 (27.03%), Postives = 184/344 (53.49%), Query Frame = 3
Query: 1002 DLIFVLDASSSLALYWDQELA--FTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDTTDIAQDACQIPKP--KRICS-AKLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIY--DDILKKVESLKHVGGFTRIDKAL-NLASRSFYLKSKNSKRLVVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILST 2009
D++F+LD S S++ D ELA FT +++ ++ +++ + + D E+ + E+ V+ L +T I AL + +E R+ +K V+L TDG +N P V A+KLK+ ++++ IG+G + ++L +++S +E + + L + + C IP P ++ C+ + D+ +I+D S+ + ++ F +F+ FELG K R +++SF++E + FT Y DD++ KV+SL GG T I KAL + S+ + N ++++T+G++ + ++ A L+ G +I ++G+G++ + +ELK++S+
Sbjct: 41 DVLFILDGSQSIS-KDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKEMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYP-APYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGL-LKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISS 381 HSP 4 Score: 106.301 bits (264), Expect = 9.376e-24 Identity = 92/360 (25.56%), Postives = 186/360 (51.67%), Query Frame = 3
Query: 2130 DIIFVVDKSTDNTNEIWDGIRSL---MLTTLQSLNXXXXXXXXXXXDYSDETTTEFRLDEAFTK-RDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQ-RQDECTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEK 3194
D++F++D S + + ++ + M+T +++ N + I D + E EF + +TK +++ + + R+ + + ALN + R +V K+VVLFTD A+ LK V+I+ +G+ + N LAS S+++E+ + K + Q+ + IC + CT+++ D++V +D S S+ E+++ +F+ + + N +L ++ R+ + +F+ E+ + +++ DM ++ + G T IG A+ L ++ R +VP ++++TDG N++ P A + K+ G IIS+G+G++I+ KEL I+S + V+T+ DL+K
Sbjct: 41 DVLFILDGSQSISKDDFELAKKFTKDMMTIIKNSNTENRIAFIKFGDDA-EIVFEF---QTYTKLKEMLLDVTNTRNLYSSTGIGKALNLTLKEVYPTMRKEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIGSDININELTRLASPGLSLQVESFTGLLKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFPRPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVETYGDLKK 396 HSP 5 Score: 67.781 bits (164), Expect = 2.995e-11 Identity = 43/151 (28.48%), Postives = 81/151 (53.64%), Query Frame = 3
Query: 117 ALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
L++ C + +D+ VILDSS+SI+ +++ + +FLS F + P RV+ + + D ++ +F FT + S + V+ K GG + + G+AL ++ + RP + +LI++DG N + A +++ KG I ++ IG
Sbjct: 221 GLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGI-GKALREVDSKLISQQRPNVPFN--ILIITDGVNNMFPRPLEYANRLKMKGANIISLGIG 368 HSP 6 Score: 61.6178 bits (148), Expect = 2.316e-9 Identity = 46/155 (29.68%), Postives = 82/155 (52.90%), Query Frame = 3
Query: 111 ILALDQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNT--RVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
I +L C RD+ ILD S SI+ +F+ + ++ +I +NT R++F+ +GD + F F +T + V R + S+ +G+ALN TL ++ P MR E+++++++ +DG+N + A K++E V I T+ IG
Sbjct: 28 ITSLTAACTFIIRDVLFILDGSQSISKDDFELAKKFTKDMMT--IIKNSNTENRIAFIKFGDDAEIVFEFQTYTKLKEMLLDVTNTRNLYS-STGIGKALNLTLKEVYPTMR--KEVEKLVVLFTDGTNNMYPAPYVYADKLKEANVQILTIGIG 177 HSP 7 Score: 56.225 bits (134), Expect = 1.149e-7 Identity = 47/188 (25.00%), Postives = 90/188 (47.87%), Query Frame = 3
Query: 924 FETKRTTTSNYIEISTP--DQNCNNQQLDLIFVLDASSSLALY-WDQELAFTNNVIKSLQIDIKFSQVGVLIYSDILEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNS-PTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIE 1475
+T+ S+ I P ++ C +Q DL+ +LD+S+S++ ++Q F + + ++ + ++V +L +SD + ++ V L GAT I ALR + IS+ R ++++TDG +N P +P A +LK G +I +G+G + +L +SS + +E
Sbjct: 204 LKTQSQVVSHICPIPDPGLEKPCTLEQQDLVVILDSSNSISEEDYEQGKNFLVKFLSNFELGPEKNRVAMLSFSDEIRFDFGFTDYYKSDDMINKVKSLIKFGGATGIGKALREVDSKLISQQRPNVPFNILIITDGVNNMFP--RPLEYANRLKMKGANIISLGIGEEINLKELKMISSEDKILTVE 389
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000046770.1 (SMESG000046770.1) HSP 1 Score: 98.5969 bits (244), Expect = 1.239e-21 Identity = 84/318 (26.42%), Postives = 150/318 (47.17%), Query Frame = 3
Query: 2262 YSDETTTEFRLDEAFTKRDIYVAIKQLRSNGNGGDLNTALNRARQVFKIDYRADVAKIVVLFTDKLPTNQKDSLSEAEILKGDGVKIIFVGLSKNSPYNFYENLAXXXXXXXXXXXXXXXXXXXXXXXMICDAFKQRQDE-CTMKKLDVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTI 3212
+ D+ T F D+ D+ +K +R++ + AL + R D + V+LFTD A+ LK V+I+ VG+ NLAS +I + + K+ +N +I + +C +E C +++ D++V +D S S+ +F+I FV ++ + TR+G+ +F+ I+ + D K+I + + T +G A+ + R +P +++LTDG N++ P A K K G IIS+G+G+ I+ KEL DI+S+ + V ++ L+K N KT+
Sbjct: 19 FGDDATLIFNFDKFKNLNDMVRQVKNVRNDNGATAIGKALKLTYENLVPQMRKDAQRYVILFTDGTNNVFPAPYIYADHLKDASVRILTVGIGSEINKKELINLASPNMAITVADFQKLFENLMKIIHHVCVIVDPIPNEPCKVEQQDLVVLMDSSKSISGPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPR----IEFDLHLVDRNKKIKHLRKLGSATGLGLALREVQFKFWPHRRHGIPFNILILTDGYNNVFPRPHEIASKLKREGANIISLGIGRHINLKELTDISSNDKILTVNSYERLQK-NMKTV 331 HSP 2 Score: 98.2117 bits (243), Expect = 1.608e-21 Identity = 84/320 (26.25%), Postives = 150/320 (46.88%), Query Frame = 3
Query: 2814 RIGIFTFNSEVYLNVPIDSNFENA-DMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEAC----QIPQKPTICKA-AGDLIFVMGSSANKTSERFKQMSDFMKSLTKLTVVDKENTRVAILKFNRGNYIDSGFKEDYKNFESSIEDMTTSTNGVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQR 3755
RI F + L D F+N DM +++ + G TAIG A+ + L + R+D + +++ TDG N++ +P I A K ++I++VG+G +I++KEL ++AS V F L + N I + C IP +P CK DL+ +M SS + + F+ F+ L + + TRV ++ F+ I+ F + I+ + + +G AL + F R ++I+ DG E A+ LK++G + ++G+ + +L ++S DK L V+++ LQ+
Sbjct: 13 RISFIVFGDDATLIFNFDK-FKNLNDMVRQVKNVRNDNGATAIGKALKLTYENLVPQMRKDAQRYVILFTDGTNNVFPAPYIYADHLKDASVRILTVGIGSEINKKELINLASPNMAITVADFQKLFE-NLMKIIHHVCVIVDPIPNEP--CKVEQQDLVVLMDSSKSISGPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIE--FDLHLVDRNKKIKHLRKLGSATGLGLALREVQFKFWPHRRHGIPFNILILTDGYNNVFPRPHEIASKLKREGANIISLGIGRHINLKELTDISSNDKILTVNSYERLQK 326 HSP 3 Score: 94.7449 bits (234), Expect = 2.360e-20 Identity = 73/268 (27.24%), Postives = 134/268 (50.00%), Query Frame = 3
Query: 1224 GATFISNALRRSGEEFISKGREGAKKTVILVTDGADNS-PTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTETYYPIEDMSKMALFDT-TDIAQDACQI--PKPKRICSA-KLDIALIVDCSSQVQLHEWVNQLLFAKRFIEQFELGINKTRFSLVSFANEAVVEEEFTHIYDDILKKVESLKHVGGFTRIDKALNLASRSFYLKSKNSKRL-VVLLTNGLSMERVQTIQAAYDLQESGVDIATIGLGDKADREELKILST 2009
GAT I AL+ + E + + R+ A++ VIL TDG +N P P + A+ LK +R++ +G+G + K +L ++S + D K LF+ I C I P P C + D+ +++D S + ++ F I +F++G TR LVSF++ +E + H+ D KK++ L+ +G T + AL F+ ++ +++LT+G + + + A L+ G +I ++G+G + +EL +S+
Sbjct: 50 GATAIGKALKLTYENLVPQMRKDAQRYVILFTDGTNNVFPA--PYIYADHLKDASVRILTVGIGSEINKKELINLASPNMAITVADFQK--LFENLMKIIHHVCVIVDPIPNEPCKVEQQDLVVLMDSSKSISGPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIEFDL-HLVDRN-KKIKHLRKLGSATGLGLALREVQFKFWPHRRHGIPFNILILTDGYNNVFPRPHEIASKLKREGANIISLGIGRHINLKELTDISS 311 HSP 4 Score: 93.5893 bits (231), Expect = 5.144e-20 Identity = 69/246 (28.05%), Postives = 120/246 (48.78%), Query Frame = 3
Query: 3435 EDYKNFESSIEDMTTSTN---GVFIGKALTSARVLFKSDTRKNATKTVIIIIDGEITDRNETFEEANLLKKDGIKVFTIGLTGKTSESDLKSVASPDKALFVDNFSNLQRYNAYISSELCDVIKG-TNKECSTKQLDLMFALDSSSSI-GPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDG 4157
+ +KN + + N IGKAL RK+A + VI+ DG + A+ LK +++ T+G+ + ++ +L ++ASP+ A+ V +F L I +C ++ N+ C +Q DL+ +DSS SI GPD+ + FV ++ I T+VG+V+F+ R EF L ++ I ++++ AT +G ALRE + + R +P I+++TDG
Sbjct: 30 DKFKNLNDMVRQVKNVRNDNGATAIGKALKLTYENLVPQMRKDAQRYVILFTDGTNNVFPAPYIYADHLKDASVRILTVGIGSEINKKELINLASPNMAITVADFQKLFENLMKIIHHVCVIVDPIPNEPCKVEQQDLVVLMDSSKSISGPDF-EIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIEFDL-HLVDRNKKIKHLRKL---GSATGLGLALREVQFKFWPHRRHGIPFNILILTDG 270 HSP 5 Score: 61.2326 bits (147), Expect = 2.179e-9 Identity = 48/166 (28.92%), Postives = 85/166 (51.20%), Query Frame = 3
Query: 972 PDQNCNNQQLDLIFVLDASSSLALYWDQELA--FTNNVIKSLQIDIKFSQVGVLIYSDI--LEMSLPLGSKQNKTEIQKFVSKLELLEGATFISNALRRSGEEFISKGREGAKKTVILVTDGADNSPTMKPEVEAEKLKKMGIRVIVIGLGLSVIKDQLNKMSSTE 1457
P++ C +Q DL+ ++D+S S++ D E+ F ++I +I K ++VG++ +SD +E L L + K + L L AT + ALR +F R G ++++TDG +N +P A KLK+ G +I +G+G + +L +SS +
Sbjct: 156 PNEPCKVEQQDLVVLMDSSKSIS-GPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIEFDLHLVDRNKK------IKHLRKLGSATGLGLALREVQFKFWPHRRHGIPFNILILTDGYNNV-FPRPHEIASKLKREGANIISLGIGRHINLKELTDISSND 313 HSP 6 Score: 56.6102 bits (135), Expect = 7.515e-8 Identity = 42/149 (28.19%), Postives = 73/149 (48.99%), Query Frame = 3
Query: 123 DQRCIGKPRDIAVILDSSSSITNGEFQSSLIAINQFLSFFLIMPNNTRVSFMGYGDTLKTEFLFTKHTSSPTLNFAVRLHRKVGGDSSVLGRALNSTLYDIVPEMRPTSEIDRILLILSDGSNTDELSVKDLATKIQEKGVTIYTVAIG 569
++ C + +D+ V++DSS SI+ +F+ + +S F I P TRV + + D + EF H N ++ RK+G + LG AL + P R + +LIL+DG N ++A+K++ +G I ++ IG
Sbjct: 157 NEPCKVEQQDLVVLMDSSKSISGPDFEIGKRFVAHLISKFKIGPKATRVGLVSFSDHPRIEFDL--HLVD--RNKKIKHLRKLGSATG-LGLALREVQFKFWPHRRHGIPFN--ILILTDGYNNVFPRPHEIASKLKREGANIISLGIG 298
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Match: SMESG000033673.1 (SMESG000033673.1) HSP 1 Score: 88.1965 bits (217), Expect = 5.153e-17 Identity = 56/176 (31.82%), Postives = 92/176 (52.27%), Query Frame = 3
Query: 2700 DVIVTIDKSTSLGPENFQIELDFVNRIVQNLDLRKDRTRIGIFTFNSEVYLNVPIDSNFENADMQKEINKIPFIEGNTAIGWAIGNATDYLFTKGREDVPKVLVVLTDGANDIGESPQISAVKAKLGGIKIISVGVGQQISEKELNDIASSGEFYNVKTFTDLEKFNAKTISNEAC 3227
+VI +D STS+GP+NF E DF +IV L + + TR+ + T+NS L +D + D+ +EI K F EG T +G A+ + +K R KV+++++DG + G P + A + K +I++G+ ++ E + I+ FY +K F L K TI + C
Sbjct: 47 EVIFILDGSTSVGPDNFNKEKDFATQIVNRLVIGNNLTRVALITYNSVPQLVFNVDKYNNSKDVNREIMKAEFTEGITVVGDALNMVLTDIESKTRRSTGKVIILISDGKRNGGMDPILVAKQLKALSYMLITIGLDKETDENSMIAISDKKNFYLIKDFDSLPKI-VSTIVQKTC 221 HSP 2 Score: 82.4185 bits (202), Expect = 2.658e-15 Identity = 55/181 (30.39%), Postives = 98/181 (54.14%), Query Frame = 3
Query: 3807 KECSTKQLDLMFALDSSSSIGPDYWQTELDFVERIVRDMDINANFTKVGVVTFNTDARKEFGLDQFTEKYAMISAVKRIIFDDGATDIGEALRECKNELLNSTRKEVPKVIVLVTDGVSTGGLSPELESTXXXXXXXXXXXXXXXDSTSPSQLKILSSSGTFYRILDFKTLKDYDTTFITR 4349
K+C +++F LD S+S+GPD + E DF +IV + I N T+V ++T+N+ + F +D++ + + + F +G T +G+AL ++ + TR+ KVI+L++DG GG+ P L + +K L +I IG+ T + + +S FY I DF +L +T + +
Sbjct: 39 KKCIKTAGEVIFILDGSTSVGPDNFNKEKDFATQIVNRLVIGNNLTRVALITYNSVPQLVFNVDKYNNSKDVNREIMKAEFTEGITVVGDALNMVLTDIESKTRRSTGKVIILISDGKRNGGMDPILVAKQLKALSYMLITIGLDKETDENSMIAISDKKNFYLIKDFDSLPKIVSTIVQK 219 The following BLAST results are available for this feature:
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Collagen alpha-6(VI) chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30018052 ID=SMED30018052|Name=Collagen alpha-6(VI) chain|organism=Schmidtea mediterranea sexual|type=transcript|length=4466bpback to top protein sequence of SMED30018052-orf-1 >SMED30018052-orf-1 ID=SMED30018052-orf-1|Name=SMED30018052-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1441bp MILIWSCLIVVACINFEVILALDQRCIGKPRDIAVILDSSSSITNGEFQSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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